LOCUS       DAZPJP010000001        75816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPJP010000001 DAZPJP010000000
VERSION     DAZPJP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.001"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(118..1368)
                     /gene="icd"
                     /locus_tag="ACHU9J_000001"
     CDS             complement(118..1368)
                     /gene="icd"
                     /locus_tag="ACHU9J_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1903673.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1540..2193
                     /gene="rluE"
                     /locus_tag="ACHU9J_000002"
     CDS             1540..2193
                     /gene="rluE"
                     /locus_tag="ACHU9J_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1903674.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2203..2664
                     /gene="nudJ"
                     /locus_tag="ACHU9J_000003"
     CDS             2203..2664
                     /gene="nudJ"
                     /locus_tag="ACHU9J_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1903675.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2718..3824
                     /gene="mnmA"
                     /locus_tag="ACHU9J_000004"
                     /gene_synonym="trmU"
     CDS             2718..3824
                     /gene="mnmA"
                     /locus_tag="ACHU9J_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1903676.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3860..4501
                     /gene="hflD"
                     /locus_tag="ACHU9J_000005"
     CDS             3860..4501
                     /gene="hflD"
                     /locus_tag="ACHU9J_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1903677.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4505..5875
                     /gene="purB"
                     /locus_tag="ACHU9J_000006"
     CDS             4505..5875
                     /gene="purB"
                     /locus_tag="ACHU9J_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1903678.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6043..6714
                     /gene="phoP"
                     /locus_tag="ACHU9J_000007"
     CDS             6043..6714
                     /gene="phoP"
                     /locus_tag="ACHU9J_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1903679.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6714..8174
                     /gene="phoQ"
                     /locus_tag="ACHU9J_000008"
     CDS             6714..8174
                     /gene="phoQ"
                     /locus_tag="ACHU9J_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1903680.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8250..9371
                     /gene="roxA"
                     /locus_tag="ACHU9J_000009"
                     /gene_synonym="ycfD"
     CDS             8250..9371
                     /gene="roxA"
                     /locus_tag="ACHU9J_000009"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1903681.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9420..10646)
                     /gene="pepT"
                     /locus_tag="ACHU9J_000010"
     CDS             complement(9420..10646)
                     /gene="pepT"
                     /locus_tag="ACHU9J_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1903682.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10896..12032
                     /gene="potA"
                     /locus_tag="ACHU9J_000011"
     CDS             10896..12032
                     /gene="potA"
                     /locus_tag="ACHU9J_000011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1903683.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12016..12873
                     /gene="potB"
                     /locus_tag="ACHU9J_000012"
     CDS             12016..12873
                     /gene="potB"
                     /locus_tag="ACHU9J_000012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1903684.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12870..13664
                     /gene="potC"
                     /locus_tag="ACHU9J_000013"
     CDS             12870..13664
                     /gene="potC"
                     /locus_tag="ACHU9J_000013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1903685.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13661..14707
                     /gene="potD"
                     /locus_tag="ACHU9J_000014"
     CDS             13661..14707
                     /gene="potD"
                     /locus_tag="ACHU9J_000014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1903686.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14863..15684)
                     /gene="cobB"
                     /locus_tag="ACHU9J_000015"
     CDS             complement(14863..15684)
                     /gene="cobB"
                     /locus_tag="ACHU9J_000015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1903687.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15700..16611)
                     /gene="nagK"
                     /locus_tag="ACHU9J_000016"
     CDS             complement(15700..16611)
                     /gene="nagK"
                     /locus_tag="ACHU9J_000016"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1903688.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16640..17884)
                     /gene="lolE"
                     /locus_tag="ACHU9J_000017"
     CDS             complement(16640..17884)
                     /gene="lolE"
                     /locus_tag="ACHU9J_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1903689.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17884..18585)
                     /gene="lolD"
                     /locus_tag="ACHU9J_000018"
     CDS             complement(17884..18585)
                     /gene="lolD"
                     /locus_tag="ACHU9J_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1903690.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18578..19777)
                     /gene="lolC"
                     /locus_tag="ACHU9J_000019"
     CDS             complement(18578..19777)
                     /gene="lolC"
                     /locus_tag="ACHU9J_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1903691.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20039..21112
                     /locus_tag="ACHU9J_000020"
     CDS             20039..21112
                     /locus_tag="ACHU9J_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1903692.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21240..24686
                     /gene="mfd"
                     /locus_tag="ACHU9J_000021"
     CDS             21240..24686
                     /gene="mfd"
                     /locus_tag="ACHU9J_000021"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1903693.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24830..25786
                     /gene="ldtC"
                     /locus_tag="ACHU9J_000022"
                     /pseudo
     CDS             24830..25786
                     /gene="ldtC"
                     /locus_tag="ACHU9J_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25869..26126)
                     /gene="bhsA"
                     /locus_tag="ACHU9J_000023"
                     /gene_synonym="ycfR"
     CDS             complement(25869..26126)
                     /gene="bhsA"
                     /locus_tag="ACHU9J_000023"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1903694.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26367..26999
                     /gene="comR"
                     /locus_tag="ACHU9J_000024"
     CDS             26367..26999
                     /gene="comR"
                     /locus_tag="ACHU9J_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1903695.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27061..27600)
                     /locus_tag="ACHU9J_000025"
     CDS             complement(27061..27600)
                     /locus_tag="ACHU9J_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1903696.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(27827..29131)
                     /gene="ndh"
                     /locus_tag="ACHU9J_000026"
     CDS             complement(27827..29131)
                     /gene="ndh"
                     /locus_tag="ACHU9J_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1903697.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29539..30081)
                     /gene="ycfP"
                     /locus_tag="ACHU9J_000027"
     CDS             complement(29539..30081)
                     /gene="ycfP"
                     /locus_tag="ACHU9J_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1903698.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30104..31129)
                     /gene="nagZ"
                     /locus_tag="ACHU9J_000028"
     CDS             complement(30104..31129)
                     /gene="nagZ"
                     /locus_tag="ACHU9J_000028"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1903699.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31140..31964)
                     /gene="thiK"
                     /locus_tag="ACHU9J_000029"
     CDS             complement(31140..31964)
                     /gene="thiK"
                     /locus_tag="ACHU9J_000029"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1903700.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31945..32586)
                     /gene="lpoB"
                     /locus_tag="ACHU9J_000030"
     CDS             complement(31945..32586)
                     /gene="lpoB"
                     /locus_tag="ACHU9J_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1903701.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32600..32977)
                     /locus_tag="ACHU9J_000031"
     CDS             complement(32600..32977)
                     /locus_tag="ACHU9J_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1903702.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32980..33339)
                     /gene="hinT"
                     /locus_tag="ACHU9J_000032"
     CDS             complement(32980..33339)
                     /gene="hinT"
                     /locus_tag="ACHU9J_000032"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1903703.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            33673..35862
                     /gene="fhuE"
                     /locus_tag="ACHU9J_000033"
     CDS             33673..35862
                     /gene="fhuE"
                     /locus_tag="ACHU9J_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1903704.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35922..37355)
                     /gene="ptsG"
                     /locus_tag="ACHU9J_000034"
     CDS             complement(35922..37355)
                     /gene="ptsG"
                     /locus_tag="ACHU9J_000034"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1903705.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(37650..38447)
                     /locus_tag="ACHU9J_000035"
     CDS             complement(37650..38447)
                     /locus_tag="ACHU9J_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1903706.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38458..39462)
                     /gene="holB"
                     /locus_tag="ACHU9J_000036"
     CDS             complement(38458..39462)
                     /gene="holB"
                     /locus_tag="ACHU9J_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1903707.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39459..40100)
                     /gene="tmk"
                     /locus_tag="ACHU9J_000037"
     CDS             complement(39459..40100)
                     /gene="tmk"
                     /locus_tag="ACHU9J_000037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1903708.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40090..41112)
                     /gene="yceG"
                     /locus_tag="ACHU9J_000038"
     CDS             complement(40090..41112)
                     /gene="yceG"
                     /locus_tag="ACHU9J_000038"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1903709.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41115..41924)
                     /gene="pabC"
                     /locus_tag="ACHU9J_000039"
     CDS             complement(41115..41924)
                     /gene="pabC"
                     /locus_tag="ACHU9J_000039"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1903710.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(42044..43285)
                     /gene="fabF"
                     /locus_tag="ACHU9J_000040"
     CDS             complement(42044..43285)
                     /gene="fabF"
                     /locus_tag="ACHU9J_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1903711.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43373..43609)
                     /gene="acpP"
                     /locus_tag="ACHU9J_000041"
     CDS             complement(43373..43609)
                     /gene="acpP"
                     /locus_tag="ACHU9J_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1903712.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(43820..44554)
                     /gene="fabG"
                     /locus_tag="ACHU9J_000042"
     CDS             complement(43820..44554)
                     /gene="fabG"
                     /locus_tag="ACHU9J_000042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1903713.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44567..45496)
                     /gene="fabD"
                     /locus_tag="ACHU9J_000043"
     CDS             complement(44567..45496)
                     /gene="fabD"
                     /locus_tag="ACHU9J_000043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1903714.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45512..46465)
                     /gene="fabH"
                     /locus_tag="ACHU9J_000044"
     CDS             complement(45512..46465)
                     /gene="fabH"
                     /locus_tag="ACHU9J_000044"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1903715.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIDKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46533..47603)
                     /gene="plsX"
                     /locus_tag="ACHU9J_000045"
     CDS             complement(46533..47603)
                     /gene="plsX"
                     /locus_tag="ACHU9J_000045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1903716.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(47684..47857)
                     /gene="rpmF"
                     /locus_tag="ACHU9J_000046"
     CDS             complement(47684..47857)
                     /gene="rpmF"
                     /locus_tag="ACHU9J_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1903717.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(47909..48430)
                     /gene="yceD"
                     /locus_tag="ACHU9J_000047"
     CDS             complement(47909..48430)
                     /gene="yceD"
                     /locus_tag="ACHU9J_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1903718.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48629..49213
                     /gene="yceF"
                     /locus_tag="ACHU9J_000048"
     CDS             48629..49213
                     /gene="yceF"
                     /locus_tag="ACHU9J_000048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1903719.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49325..50284)
                     /gene="rluC"
                     /locus_tag="ACHU9J_000049"
     CDS             complement(49325..50284)
                     /gene="rluC"
                     /locus_tag="ACHU9J_000049"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1903720.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            50857..54042
                     /gene="rne"
                     /locus_tag="ACHU9J_000050"
     CDS             50857..54042
                     /gene="rne"
                     /locus_tag="ACHU9J_000050"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1903721.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54237..55190)
                     /gene="flgL"
                     /locus_tag="ACHU9J_000051"
     CDS             complement(54237..55190)
                     /gene="flgL"
                     /locus_tag="ACHU9J_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1903722.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55202..56845)
                     /gene="flgK"
                     /locus_tag="ACHU9J_000052"
     CDS             complement(55202..56845)
                     /gene="flgK"
                     /locus_tag="ACHU9J_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1903723.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(56911..57852)
                     /gene="flgJ"
                     /locus_tag="ACHU9J_000053"
     CDS             complement(56911..57852)
                     /gene="flgJ"
                     /locus_tag="ACHU9J_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1903724.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(57852..58949)
                     /gene="flgI"
                     /locus_tag="ACHU9J_000054"
     CDS             complement(57852..58949)
                     /gene="flgI"
                     /locus_tag="ACHU9J_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1903725.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(58961..59659)
                     /gene="flgH"
                     /locus_tag="ACHU9J_000055"
     CDS             complement(58961..59659)
                     /gene="flgH"
                     /locus_tag="ACHU9J_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1903726.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(59712..60484)
                     /gene="flgG"
                     /locus_tag="ACHU9J_000056"
                     /pseudo
     CDS             complement(59712..60484)
                     /gene="flgG"
                     /locus_tag="ACHU9J_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(60656..61411)
                     /gene="flgF"
                     /locus_tag="ACHU9J_000057"
     CDS             complement(60656..61411)
                     /gene="flgF"
                     /locus_tag="ACHU9J_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1903727.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61431..62636)
                     /gene="flgE"
                     /locus_tag="ACHU9J_000058"
     CDS             complement(61431..62636)
                     /gene="flgE"
                     /locus_tag="ACHU9J_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1903728.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(62661..63356)
                     /gene="flgD"
                     /locus_tag="ACHU9J_000059"
     CDS             complement(62661..63356)
                     /gene="flgD"
                     /locus_tag="ACHU9J_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1903729.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63368..63772)
                     /gene="flgC"
                     /locus_tag="ACHU9J_000060"
     CDS             complement(63368..63772)
                     /gene="flgC"
                     /locus_tag="ACHU9J_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1903730.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(63776..64192)
                     /gene="flgB"
                     /locus_tag="ACHU9J_000061"
     CDS             complement(63776..64192)
                     /gene="flgB"
                     /locus_tag="ACHU9J_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1903731.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64347..65006
                     /gene="flgA"
                     /locus_tag="ACHU9J_000062"
     CDS             64347..65006
                     /gene="flgA"
                     /locus_tag="ACHU9J_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1903732.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65082..65375
                     /gene="flgM"
                     /locus_tag="ACHU9J_000063"
     CDS             65082..65375
                     /gene="flgM"
                     /locus_tag="ACHU9J_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1903733.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65380..65796
                     /gene="flgN"
                     /locus_tag="ACHU9J_000064"
     CDS             65380..65796
                     /gene="flgN"
                     /locus_tag="ACHU9J_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1903734.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(65836..67371)
                     /gene="murJ"
                     /locus_tag="ACHU9J_000065"
     CDS             complement(65836..67371)
                     /gene="murJ"
                     /locus_tag="ACHU9J_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1903735.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67481..68404)
                     /locus_tag="ACHU9J_000066"
     CDS             complement(67481..68404)
                     /locus_tag="ACHU9J_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1903736.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68406..69053)
                     /locus_tag="ACHU9J_000067"
     CDS             complement(68406..69053)
                     /locus_tag="ACHU9J_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1903737.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69064..69648)
                     /gene="rimJ"
                     /locus_tag="ACHU9J_000068"
     CDS             complement(69064..69648)
                     /gene="rimJ"
                     /locus_tag="ACHU9J_000068"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1903738.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            69884..71092
                     /gene="mdtH"
                     /locus_tag="ACHU9J_000069"
     CDS             69884..71092
                     /gene="mdtH"
                     /locus_tag="ACHU9J_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1903739.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71156..71802
                     /gene="grxB"
                     /locus_tag="ACHU9J_000070"
                     /pseudo
     CDS             71156..71802
                     /gene="grxB"
                     /locus_tag="ACHU9J_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
     gene            71936..72496
                     /locus_tag="ACHU9J_000071"
     CDS             71936..72496
                     /locus_tag="ACHU9J_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1903740.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72602..73648
                     /gene="pyrC"
                     /locus_tag="ACHU9J_000072"
     CDS             72602..73648
                     /gene="pyrC"
                     /locus_tag="ACHU9J_000072"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1903741.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            73722..73967
                     /gene="dinI"
                     /locus_tag="ACHU9J_000073"
     CDS             73722..73967
                     /gene="dinI"
                     /locus_tag="ACHU9J_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1903742.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74257..74511
                     /gene="bssS"
                     /locus_tag="ACHU9J_000074"
     CDS             74257..74511
                     /gene="bssS"
                     /locus_tag="ACHU9J_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1903743.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74626..75744
                     /gene="solA"
                     /locus_tag="ACHU9J_000075"
     CDS             74626..75744
                     /gene="solA"
                     /locus_tag="ACHU9J_000075"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1903744.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18427 a        20169 c        18968 g        18252 t
ORIGIN      
        1 tatgggctca agtaatgggc ttcatataaa tatgctcgat atgctattaa taatatccac
       61 ataaataaaa caacgggcgt gttatacgcc cgtttcaata tttaactcat ggagagatta
      121 catgttttcg atgatcgctt caccaaactc tgaacatttc agcagcttag cgccttccat
      181 cagacgttca aagtcataag ttacggtttt cgcgttgata gcaccttcca tacctttaac
      241 aattaaatca gccgcttcag tccaccccat atggcgcagc atcatctcag cggagaggat
      301 gatagagcct ggatttactt tgtcctgacc ggcatatttc ggcgcagtac cgtgggtggc
      361 ttcaaacagg gcgcattcgt caccgatgtt tgcgccaggg gcgataccga taccgccaac
      421 ctgcgctgcc agggcgtcag aaatgtagtc accgttcagg ttcatacagg cgataacatc
      481 atattcagcc ggacgcagca ggatctgttg caggaatgca tcagcaatca cgtctttaat
      541 gacgatctct ttgccggtgt tcgggttttt aactttcagc cacgggccgc cgtcgatcag
      601 ttcaccgcca aactcttcac gcgccagctg gtagccccag tctttaaacg cgccttcggt
      661 gaacttcatg atgttgcctt tgtgcaccag agtcacagag tcacgatcgt tagcaattgc
      721 gtattcgatc gctgcacgaa ccagacgttt ggtgccttct tccgaacacg gcttaatacc
      781 gataccacaa tgttccggga agcgaatttt cttcaccccc atctcttcac gcaggaattt
      841 aatcactttc tcggcgtcgg cagagtctgc tttccattcg atacccgcat aaatgtcttc
      901 cgagttttca cggaagataa ccatatcggt cagttcaggg tgtttaaccg ggcttggagt
      961 gccctgatag taacgtaccg gacgcaggca gatgtagaga tccagttcct ggcgcagggc
     1021 aacgttcaga gagcgaatac cgccaccaac aggagtggtc agcggacctt taatggcaac
     1081 gcgatattca cgaatcagat caagggtttc agcaggcagc cagacatcct gaccataaac
     1141 ctgtgtggat ttttcaccgg tgtaaatttc catccaggag attttacgct cgcctttata
     1201 ggctttctcg actgcagcgt cgaccacttt cagcatggct ggggttacat ctacaccgat
     1261 tccatcacct tcaatgtaag ggataatcgg attttcagga acgttgagtt tgccgttttg
     1321 cagggtgatc ttcttgcctt gtgccggaac aactacttta ctttccattc acctctcctt
     1381 cgagcgctac tggtttgctc gtctgccacc acgttgcata tgcgtttgcg tcctgcgata
     1441 cggatgcttt agagcaattt tttgttaatg atttgtaatt ggcttgtcaa tgctacccta
     1501 ttgtttgccg tcatgaaaga tgcgcgttat taggctataa tgcggcaatt cataatctct
     1561 gaaaatacca tgcaaaaaac ttcttttaga aatcaccagg ttaagcgatt cagctcgcaa
     1621 cgttctacta ggcgtaaacc tgaaaaccag cccacgcgtg tgatcctgtt caataaaccc
     1681 tacgatgttc ttccgcagtt caccgatgaa gccggacgca aaacattaaa agaattcatc
     1741 ccggttcagg gcgtttatgc agcaggtcgt cttgaccgcg atagcgaagg gttactggtg
     1801 ctgaccaata acggcgcgtt gcaggcgcgt ttaacccagc cgggcaaacg caccgggaaa
     1861 atctattatg tgcaggtgga aggtattccc acacaagacg cacttgaagc cttgcgcaat
     1921 ggcgtaacct taaatgatgg ccctaccctg cccgccggcg cggagctggt tgacgaacca
     1981 gcgtggttat ggccgcggaa tccaccaatt cgtgaacgca aaagtattcc caccagctgg
     2041 ctgaagatca ccttatatga aggacgtaat cgccaggtgc gccgcatgac cgcccatgtt
     2101 ggtttcccca cgctgcgact gattcgttat gcgatgggtg attacacttt ggataacctt
     2161 gccaatggcg aatggcgaga agcaacagaa taaggatgca caatgtttaa accgcacgtt
     2221 accgttgctt gcgtagtgca cgcagagggt aaatttttag tcgttgaaga gacgattaat
     2281 ggtaaagcgt tatggaacca acctgccggg catctggaag ccgatgaaac cttagtggaa
     2341 gctgccgccc gtgagctatg ggaagaaacc ggcatcagcg cgcaaccgca acactttatt
     2401 cgtatgcatc agtggattgc gccagataaa acgccgtttt tgcgtttcct ctttgccatt
     2461 gagcttgagc aaatatgccc gacccagcct catgacagcg atatcgactg ctgtcgttgg
     2521 gtcagcgccg aagaaatttt aaaggcgtca aatcttcgtt cgccgctggt ggcggaaagt
     2581 atccgttgtt atcaaagtgg gcaacgctat ccgctggaga tgattggcga ttttaactgg
     2641 ccttttacaa agggtgtcat ctaaaaggct gcgtgataga atacgccgcc ttgaagttca
     2701 atgtcgtgag tgatccaatg tctgaaaccg caaaaaaagt aatcgtcggc atgtccggcg
     2761 gtgtcgattc ctccgtttct gcctggctgt tgcaacaaca gggatatcag gttgaaggcc
     2821 tgtttatgaa gaactgggaa gaagacgacg gtgaggaata ttgcacagcg gcagcggatc
     2881 tggctgatgc ccaggctgtc tgcgacaagc tcggcattga actgcacacc gttaactttg
     2941 ctgccgagta ctgggacaat gtcttcgaac tgttccttgc cgaatataaa gccggtcgca
     3001 cgccgaatcc ggatattctg tgcaacaaag agatcaaatt taaagctttc ctcgaatttg
     3061 ccgccgaaga tttaggtgcc gattatatcg ctaccggtca ttacgtacgt cgggccgatg
     3121 tcgatggcaa gagccgcctg ctgcgtggtc tggacagcaa taaagaccag agctacttcc
     3181 tttatacgct cagccatgag cagattgcgc aaagcctgtt cccggtcggc gaactggaaa
     3241 aaccgcaggt gcgtaagatt gctgaagatc ttggtctggt caccgcgaag aaaaaagact
     3301 ctaccggcat ttgcttcatt ggcgaacgta aattccgcga gttcctgggc cgttatctcc
     3361 cggggcaacc gggcaaaatc attaccgtcg atggcgatga aattggtgag caccaggggc
     3421 tgatgtatca cactctcggt cagcgtaaag gtctgggtat cggtggcacc aaagaaggca
     3481 ccgaagaacc ttggtatgtg gtggacaaag acgtcgaaaa caacattctg gttgtcgctc
     3541 agggccatga acacccgcgg ctgatgtctg tcgggttgat tgcccagcag ttgcactggg
     3601 tcgatcgcga accattcacc ggcactatgc gttgcacggt aaaaacccgc tatcgccaga
     3661 ccgatatccc ttgcaccgtc aaggcgctgg acgatgatcg cattgaagtg attttcgatg
     3721 aaccggttgc cgccgtgacg ccgggccagt ctgccgtctt ctataacggt gaagtgtgcc
     3781 tcggtggcgg tattattgag cagcgtctgc cgctgccggt ctgattatta tctttactta
     3841 aaacaaggaa gcagtgaacg tggcaaagaa ttactatgac atcaccctcg ccctggccgg
     3901 tatttgtcag tcggcacgcc tggtgcaaca actcgctcac caggggcatt gtgatgccga
     3961 tgcgctacac gtctcactca acagtattat tgatatgaac cccagctcga cgctggcggt
     4021 ttttggcggt agcgaagcca acctgcgcgt cgggctggaa accctgctcg gcgtgctcaa
     4081 tgccagcagt cgccagggct taaacgccga attaacccgc tacacactca gcttgatggt
     4141 gcttgagcgc aaactctcct cagcgaaagg cgcgctcgac actctgggca accggatcaa
     4201 cggcctgcaa cgccagctcg aacacttcga tttacagtcc gaaacgctga tgagcgcgat
     4261 ggctgctatc tatgttgatg tgatcagccc gcttggccca cgcattcagg tcaccggttc
     4321 ccctgctgta ctgcaaagcc cacaagtgca ggcgaaagtt cgcgcaaccc tgctggcagg
     4381 cattcgcgcc gccgtgctct ggcaccaggt cggcggcgga cgtctgcaac tgatgttttc
     4441 tcgtaatcgc ctgaccactc aggcaaaaca aattcttgct catttaaccc cggagttgtg
     4501 atctatggaa ttatcctcac tgaccgccgt ttcccctgtc gatggacgct acggcgataa
     4561 agtcagcgcg ctgcgcggga ttttcagcga atatggtttg ctgaaattcc gtgtacaagt
     4621 tgaagtacgt tggctgcaaa aactggccgc gcacgcagcg atcaaggaag ttcctgcttt
     4681 tgctgccgac gcaatcggtt accttgatgc aattgtcgcc aatttcagcg aagaagatgc
     4741 cgcacgcatc aaaaccatcg agcgtaccac taaccacgac gttaaagcgg ttgagtattt
     4801 cctgaaagaa aaagtggcgg agatcccgga actgcacgcg gtttctgaat tcatccactt
     4861 tgcctgtact tcggaagata tcaataacct ctcccacgca ttaatgctga aaaccgcgcg
     4921 tgatgaagtg atcctgccgt actggcgtca actgattgat ggcattaaag atctcgccgt
     4981 tcagtaccgc gatatcccgc tgctgtctcg tacccacggt cagccagcca cgccgtcaac
     5041 catcggtaaa gagatggcaa acgtcgccta ccgtatggag cgccagtacc gccagcttaa
     5101 ccaggtggag atcctcggca aaatcaacgg cgcggtcggt aactataacg cccacatcgc
     5161 cgcttacccg gaagttgact ggcatcagtt cagcgaagag ttcgtcacct cgctgggtat
     5221 tcaatggaac ccgtacacta cccagattga accgcacgac tacatcgccg agctgtttga
     5281 ttgcgttgca cgcttcaaca ccattctgat cgactttgat cgtgacgtct ggggttatat
     5341 cgcccttaac cacttcaaac agaaaaccat tgctggtgag attggttctt ccaccatgcc
     5401 gcataaagtt aacccgatcg acttcgagaa ctccgaaggg aatctgggcc tttccaacgc
     5461 ggtattacag catctggcaa gcaaactgcc ggtttcccgc tggcagcgtg acctgaccga
     5521 ctccaccgtg ctgcgtaacc tcggcgtggg tatcggttat gcgctgattg cgtatcaatc
     5581 caccctgaaa ggcgtgagca aactggaagt gaaccgtgac catctgctgg atgagctgga
     5641 tcacaactgg gaagtgctgg ctgagccaat ccagacagtt atgcgtcgct atggcatcga
     5701 aaaaccgtac gagaagctga aagagctgac tcgtggtaag cgcgttgacg ccgaaggcat
     5761 gaagcagttt atcgacggtc tggcgttgcc agaagaagag aaagcccgcc tgaaagcgat
     5821 gacgccggca aactacattg gtcgcgccat caccatggtt gatgagctga aataaactcg
     5881 tatcagtgcc ggatggcgat gctgtccggc ctgcttatta agattatccg ctttttattt
     5941 tttcacttta cctcccctcc ccgctggttt atttaatgtt tacccccata accacataat
     6001 cgcgttacac tattttaata attaagacag ggagaaataa aaatgcgcgt actggttgtt
     6061 gaagacaatg cgttgttacg tcaccacctt aaagttcaga ttcaggatgc tggtcatcag
     6121 gtcgatgatg cagaagatgc caaagaagcc gattattatc tcaatgaaca tttaccggat
     6181 attgcgattg tcgatctcgg attgccagac gaggacggtc tgtcactgat tcgccgctgg
     6241 cgtagcaatg atgtttcact gccgattctg gtattaaccg cccgtgaaag ctggcaggac
     6301 aaagtcgaag tattaagtgc tggtgctgat gattacgtga ctaaaccatt tcatattgaa
     6361 gaggtgatgg cgcgaatgca ggcattaatg cggcgtaata gcggtctggc ttcacaggtc
     6421 atttcgctac caccgtttca ggttgatctc tctcgccgtg aattatctat taatgacgaa
     6481 gtgatcaaac tgaccgcgtt cgaatacacc attatggaaa cgttgatacg caataatggc
     6541 aaagtggtga gcaaagattc gttaatgctc caactctatc cggatgcgga gctgcgggaa
     6601 agccatacca ttgatgtact gatgggacgt ctgcgcaaaa aaattcaggc acaatatccg
     6661 caagaagtga ttactaccgt tcgcggccag ggctatttgt tcgaattgcg ctgatgaaaa
     6721 aattactgcg tctttttttc ccgctctcgc tgcgggtacg ttttctgttg gcaacggctg
     6781 cggtagtact ggtgctttcg cttgcctacg gaatggtcgc gctgatcggt tatagcgtca
     6841 gtttcgataa aactacgttt cggctgttac gtggcgagag caatctgttc tatacccttg
     6901 cgaagtggga aaacaataag ttgcatgtcg agttacccga aaatatcgac aagcaaagcc
     6961 ccaccatgac gctaatttat gatgagaacg ggcagctttt atgggcgcaa cgtgacgtgc
     7021 cctggctgat gaagatgatc cagcctgact ggctgaaatc gaatggtttt catgaaattg
     7081 aagcggatgt taacgatacc agcctcttgc tgagtggaga tcattcgata cagcaacagt
     7141 tgcaggaagt gcgggaagat gatgacgacg cggagatgac ccactcggtg gcagtaaacg
     7201 tctacccggc aacatcgcgg atgccaaaat taaccattgt ggtggtggat accattccgg
     7261 tggagctaaa aagttcctat atggtctgga gctggtttat ctatgtgctc tcagccaatc
     7321 tgctgttagt gatcccgctg ctgtgggtcg ccgcctggtg gagtttacgc cccatcgaag
     7381 ccctggcaaa agaagtccgc gaactggaag aacataaccg cgaattgctc aatccagcca
     7441 caacgcgaga actgaccagt ctggtacgaa acctgaaccg attgttaaaa agtgaacgcg
     7501 aacgttacga caaataccgt acgacgctca ccgacctgac ccatagtctg aaaacgccac
     7561 tggcggtgct gcaaagtacg ctgcgttctc tgcgtagtga aaagatgagc gtcagtgatg
     7621 ctgagccggt aatgctggag caaatcagcc gcatttcaca gcaaattggc tactacctgc
     7681 atcgtgccag tatgcgcggc gggacattgc tcagccgcga gctgcatccg gtcgccccac
     7741 tgctggacaa tctcacctca gcgctgaaca aagtgtatca acgcaaaggg gtcaatatct
     7801 ctctcgatat ttcgccagag atcagctttg tcggtgagca gaacgatttt gtcgaggtga
     7861 tgggcaacgt gctggataat gcctgtaaat attgcctcga gtttgtcgaa atttctgcaa
     7921 ggcaaaccga cgagcatctc tatattgtgg tcgaggatga tggccccggt attccattaa
     7981 gcaagcgaga ggtcattttc gaccgtggtc aacgggttga tactttacgc cctgggcaag
     8041 gtgtagggct ggcggtagcc cgcgaaatca ccgagcaata tgagggtaaa atcgtcgccg
     8101 gagagagcat gctgggcggt gcgcggatgg aggtgatttt tggtcgccag cattctgcgc
     8161 cgaaagatga ataaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
     8221 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
     8281 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
     8341 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
     8401 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
     8461 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtgaac cactggcatg
     8521 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
     8581 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
     8641 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
     8701 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
     8761 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
     8821 cgctggaaaa tgcgatgaac tattccgtgg gctttcgcgc gccaaatacg cgggaactga
     8881 ttagtggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
     8941 ctgatgttcc acctcgcgct catcctgcgg atgttctgcc tcaagagatg gataaactgc
     9001 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
     9061 ttatatccca gtcacgtcat gaactggata tcgcgccgcc agaaccgcct tatcagccag
     9121 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
     9181 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
     9241 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
     9301 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
     9361 tcgaagggta agtttggatt taagccggat gcggcatcac atgccgcatc ccttttcgct
     9421 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
     9481 ccttccagag tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaagttc
     9541 gggcacggta atcccataaa cgacaactgc gcgccgtcgg taccaccgcg gatcggtttc
     9601 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
     9661 tgctcaacca ctttctctcg catattgtag taactgtctt caatcaccag ttcaatgtag
     9721 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
     9781 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
     9841 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
     9901 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
     9961 cctttcgccg tgcccggatg aacattgtta ccgacaattt tgatattgac cgatgcggcg
    10021 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    10081 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    10141 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    10201 atgatttctg caatacctgc tttgtcatcg gcacctaaca aggttttacc atcggtggta
    10261 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    10321 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    10381 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    10441 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    10501 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    10561 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga tacgtagttc
    10621 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    10681 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    10741 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    10801 ttcaagcggt ttgccgtaga attaccgccc tcattaagag tcaccaaggt ggttaaccac
    10861 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    10921 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    10981 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    11041 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    11101 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    11161 gctatgtgaa cactgttttc caaagctacg cacttttccc ccacatgacc gtgttcgaaa
    11221 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    11281 tggaagccct gcggatggtg caactggaaa ccttcgctca acgtaaaccg catcagctct
    11341 ctggtggtca acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt
    11401 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    11461 agctgaaagc gttacagcgt aagcttggta ttaccttcgt ctttgtcact cacgatcagg
    11521 aagaagcgct caccatgtca gacaggattg tggtgatgcg cgagggtcgt attgagcaag
    11581 atggtacacc gcgcgaaatt tacgaagagc cgaaaaacct gtttgttgcc ggcttcattg
    11641 gcgaaatcaa tatgtttaac gccactgtca tcgaacgtct cgacgagcag cgcgtacgcg
    11701 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    11761 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    11821 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    11881 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    11941 accctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    12001 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    12061 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    12121 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cgctggataa ctacacgcgt
    12181 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    12241 accctcgcct gcctggtgct gggctacccg tttgcctggt ttctggcgaa gctgccacac
    12301 aaggtgcgtc cgctgctgct gtttctgctg attgttccct tctggaccaa ctcactgatt
    12361 cgcatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    12421 tggctgggcg ttatcgacac accaattcgt atcatgttca cgcccagtgc ggtgattatc
    12481 ggtctggttt acattctgct accgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    12541 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    12601 atccgtatca ttattccact gacgatgccg ggaattattg ccggatgtct gctggtgatg
    12661 ctgccagcga tgggcctgtt ctatgtatcc gacctgatgg gcggtgcgaa aaacctgctg
    12721 atcggtaacg tcatcaaggt ccagttcctc aatattcgtg actggccgtt tggtgcagcc
    12781 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    12841 cgtctgctga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    12901 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    12961 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    13021 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    13081 cgacatttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    13141 gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat gatgtcacca gatatcgtga
    13201 tggcgatttc tctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    13261 tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt ggtgacggtg tattcgcgcc
    13321 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agtgaattta
    13381 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    13441 taagctttac cctgtcgatg gacgatgtgg tagtttcttc gttcgtcacc ggaccaagtt
    13501 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    13561 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    13621 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    13681 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    13741 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    13801 caaagaaacc ggtattaagg ctatctattc gacttacgag tcgaacgaaa ccatgtacgc
    13861 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    13921 cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    13981 cagcaatctc gatccagaca tgctcaacaa gccgtttgac ccgaataacg actactccat
    14041 tccgtatatc tggggtgcga cggcgattgg cgttaacggt gatgcggtgg atcctaaatc
    14101 tgtcaccagt tgggcagatc tgtggaagcc agagtacaaa ggtagcctgc tgctgaccga
    14161 cgatgcccgt gaagtgttcc agatggcgct gcgcaaactg ggctactccg gtaacaccac
    14221 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    14281 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    14341 gatctggaac ggctctgctt tcgttgcacg ccaggcgggt acgccaattg acgtggtgtg
    14401 gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    14461 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    14521 tgccgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagcccgga
    14581 agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg gtgaatggca
    14641 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    14701 acgttaattc agcaaacggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    14761 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtcccctt
    14821 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc ccttcagcaa
    14881 cttttcaaca aactcaggca ccacctggct tgccgggccg taatatttct cggcaaattc
    14941 attaccgacc tgacttggtt cgagattcag ttccacggtg tgcgcgccat gcagtttcgc
    15001 ttcgtgaaca aacccagccg ccggataaac atgcccggaa gtaccaatgg caatgaaaat
    15061 atcagccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    15121 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    15181 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    15241 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    15301 cgttaccagc aaaaagcgat cgccgagggc gtcttgcagt ttagccagcg caagatgcgc
    15361 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    15421 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    15481 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    15541 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    15601 aaaaatacgc tgacgcaaac gctcgcgcag gcggcgttta tttttacgaa aacgacttaa
    15661 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    15721 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
    15781 agctacaggt aagagatgac gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa
    15841 attcgataag ccaccaccaa tgacgaccag gtcagagtca acgatggtca ggatatttcc
    15901 cagacaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    15961 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgcca
    16021 gagccacgca aagccccgac ctgatagata attttcgata caaccaattt gaccacagcc
    16081 gcagcggcgt aacgggaaat ccagccccat catggttaac gcatcaaccg gcagacgcat
    16141 atggccaaac tcgccggtaa tgtagctttt accggtaatc ggtttaccgt tgaaaatcag
    16201 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    16261 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    16321 atcaagacgt gcgctcaggt cggcacgcag cggtttaccg ctggcagcgg ggacattggc
    16381 ggcatacagc gtaccatctt ctgtttccgg cattcccgga ataccgatgc caacagaacc
    16441 tttacagcca aaacgttggt cagcttcggc taccagctcg catactgcat ctaaaaatgc
    16501 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    16561 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    16621 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    16681 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    16741 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaatag atatcgctgg
    16801 agaggaactg atgaccgatc agcttttcaa tccgctcaat aatcggggta agttgcagtg
    16861 aaacaactac gccgataatc acaccacaca ggctaccgaa cagccctgcc agcaatccat
    16921 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    16981 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    17041 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    17101 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    17161 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    17221 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    17281 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    17341 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    17401 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    17461 taaaattgcg ccaggcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    17521 gttgcgggtt aacgcccttc acctgaattg cgcgcagatt ggtaccactt tctaccagtc
    17581 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttttgc acgttatcca
    17641 gtgcttcctg ccagttagtc catggctgat ttaccgcctc aatttcaccg tgcggtacca
    17701 cagcaagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    17761 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    17821 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    17881 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    17941 ttggcggctc atacgtttcg ccagttgcag gtcgtgagta accaccagga aggcggtgcc
    18001 ctgcaagcga ttcaattccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    18061 gttaccggta ggctcatccg caagtaccag acgcggatta ttgaccagcg cacgggcaat
    18121 cgccacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    18181 cagccccacc gcttttaaca tctccagcgc acggctgttg atttcagcgg gctttttctt
    18241 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    18301 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ccgaagacag
    18361 tttgctcatc ggttgaccgt taaagatcac atcgccggag gttggtgtat ccagcccgcc
    18421 cagcaggtgc agcaaggtac ttttaccgga accagagcta ccgacgatcg ccatcatttc
    18481 accttcgccg acgctgaaac tgacattgtg cagtacatcg gtttgcacac tgccttcctg
    18541 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    18601 cgggttgagt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    18661 tcgccaccag cgcaataaca atgacctgta aaggttcgat agcgaccggc agcgccgcgc
    18721 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    18781 caagcgccgc tccgaggatc gcaccgataa tcccggcgct ggccccttgc accataaaga
    18841 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    18901 tctccattac catcagccct agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca
    18961 ggctcagcag caagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    19021 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    19081 caactttcag cggctcatcc agccacaaac gccagccggt aatattgcct gccggataac
    19141 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    19201 tggcggcgaa agtgccaatc acattgaaca ggcgctggct tggaatacgc cccatcggcg
    19261 tgaactggct ggcagatggt accatcacgc ggatttgatc accgcgatta acgcctagct
    19321 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    19381 tcacattgac cagatacggc gtaagtggat ctttttgcgc cgggtcgata ccgagcatca
    19441 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    19501 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    19561 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    19621 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    19681 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt
    19741 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    19801 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    19861 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca
    19921 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    19981 tcgggtcata acccgcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat
    20041 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    20101 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    20161 agtcctgagc aaatgctgga tctatttcaa tctttacctt gcaccctttc gtatgccggt
    20221 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    20281 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    20341 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    20401 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    20461 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    20521 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    20581 tcccacgccg tggtggggaa tgaacagtgt gatccgcaat ttgccttatt acagccttgg
    20641 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    20701 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    20761 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    20821 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    20881 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    20941 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    21001 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    21061 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc
    21121 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    21181 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    21241 tgcctgaaca atatcgttat acactgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    21301 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    21361 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    21421 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    21481 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    21541 tgcagcgtgg cgtactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    21601 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    21661 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    21721 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    21781 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    21841 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    21901 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    21961 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgccgggatc gagtactggc
    22021 agccattgtt cttcagcgaa ccactgccgc cgctgttcag ttatttccct gccaatacct
    22081 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    22141 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    22201 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa
    22261 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    22321 tggccgttca ggcacaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    22381 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    22441 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    22501 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc
    22561 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    22621 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    22681 agccggtggt ccatctggag cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag
    22741 cgggcggcat tactggcgag tatttgatgc tcacctatgc caacgacgcc aaactgtatg
    22801 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    22861 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    22921 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    22981 tcgcgtttaa acacgatcgt gagcagtatc agttgttctg cgacagcttc ccgtttgaaa
    23041 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    23101 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    23161 ccgctttcct ggcagtagat aaccacaagc aggtggcggt gttggtgcct accacccttc
    23221 tcgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    23281 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    23341 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    23401 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    23461 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    23521 gcacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    23581 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    23641 cgatcctgcg tgaaattttg cgcggggggc aggtttatta tctctacaat gatgtggaaa
    23701 acatccagaa agctgccgaa cggctggcag aactggtgcc agaagcgcgg atcgccatcg
    23761 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    23821 gtttcaacgt gctggtttgt acaaccatta tcgaaaccgg gatcgacatc ccgacagcca
    23881 acaccattat cattgaacgc gcggatcact tcggtctggc gcagctgcac cagttacgcg
    23941 gtcgcgtcgg acgttcgcat catcaggcat atgcatggtt gctgacaccg catccaaaag
    24001 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    24061 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    24121 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    24181 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    24241 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatt cctgacgtga
    24301 atacgcgttt gtcgttctat aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    24361 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    24421 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    24481 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    24541 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    24601 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    24661 aactggaaga gaacgcgatc gcttaatggt aaatcggggg cgtttctgcg cccccatctg
    24721 caacatttac aaattctttg cacttccctg cactatccga cactgtcacc atccataatt
    24781 caggcttatc tgtttattac aataacctta tatttattat tgatttttgg tgatgatcaa
    24841 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctaccggc
    24901 aaaagccaac acctagccgc tgccgccagc gagcagtcgt ctggttggcg aaaacaaatt
    24961 tcatgtggtg gaaaatgacg gtggttctct ggaagccatc gccaaaaaat ataacgtcgg
    25021 ctttctcgct ctgttacagg ctaaccccgg cgttgatcct tacgtaccgc gcgcgggcag
    25081 cgtgttaacg atcccgttgc aaaccctact tccagacgcg ccgcgcgaag gcattgtgat
    25141 caacattgcg gagctgcgtc tctattacta cccgccgggt aaaaattcgg taaccgtgta
    25201 tccaataggt attggtcagt taggtggtga cacgctgaca ccgacaatgg tgaccaccgt
    25261 ttcagacaaa cgtgcaaacc caacctggac gccaacggca aacatccgcg cccgttataa
    25321 agcacaggga attgagttgc ctacggtagt gccggctgga ccggataacc caatgggcca
    25381 tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg cttcacggta cgaacgccga
    25441 tttcggcatt ggcatgcggg taagttctgg ctgtattcgt ctgcgggatg acgatatcaa
    25501 aacactcttt agccaggtca ccccaggcac caaagtgaat atcatcaaca ctccgataaa
    25561 agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta catcagccgc tgtcagagaa
    25621 gattgatgac gatccgcagc tgctgccaat tacgctgaat agcgcaatgc aatcatttaa
    25681 agatgcagca caaactgacg ctgaagtgat gcaacatgtg atggatgtcc gttccgggat
    25741 gccggtggat gtccgccgtc atcaagtgag cccacaaact ctgtaaatcc tggataaaaa
    25801 aaatccccgc gacacggcat gtcgcgggga aaaaatcagc agtagcaggc attaatgagg
    25861 gttaatgctt atttataaat tactgctgtt ccatggaggg tattcggacc ggttacagaa
    25921 gtaatacgga aagattttgc gcccatctca tccgcttttt gcgccagctg ctcttccagc
    25981 gatcccagat ttgtccccgc gttagcactg atagtaccga ctttttgttg gccttctggc
    26041 gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca tggagcttaa aatcgccgca
    26101 gcgatgaggg tttttacgtt tttcataata gtggccttat gcagatgaat gacgtcgcaa
    26161 gaaattactt aacgatcgat aactaaatga tagatgtgat ctggatcaca tacaagatat
    26221 tttttataac aatcattaat taatttaaaa atctttaaat ttcagttaaa tagaaagcaa
    26281 ttatttttat gggatctgga ctggtgaacg gaggtgatta tttttataat aacctttcat
    26341 tcacagtcac caggttcaac ggcatcatgg caactgactc aacacaatgt gtaaaaaaaa
    26401 gccgtggccg cccaaaagtg ttcgacaggg atgccgcgct tgataaggcc atgaaattgt
    26461 tctggcaaca cggttatgaa gcgacttctc ttgcggacct cgtcgaagcg accggagcca
    26521 aagcgcccac gctatacgcg gaatttacca acaaagaggg gttatttcgt gccgttctcg
    26581 accgctatat cgatcgtttt gccgctaagc atgaagcaca gctgttttgt gaagagaaaa
    26641 gcgtggagtc tgcgctggct gactattttg ctgccatcgc caactgcttt accagcaaag
    26701 acaccccggc aggctgcttc atgatcaaca actgcaccac cctctcccca gattcaggag
    26761 atatcgccaa tacgttgaaa tcacgccatg cgatgcaaga gcgcactttg cagcagtttt
    26821 tatgtcaacg acaagcgcgc ggggaaatcc cgacccactg tgacgtgaca catctggcag
    26881 aattccttaa ttgtattatt caggggatgt cgatcagcgc acgcgaaggt gcatcgctgg
    26941 aaaaactgat gcagattgcc ggaacgactt tgcgtttatg gcccgaactg gtgaaataag
    27001 tcgcaaaaaa agcccctcag cctgagcgaa tgaggggcca aattgcagag tacagccttg
    27061 ttatactttg ttattcaaaa tcagttgccc gttactatct agcgggatct gcgtacccgg
    27121 atcgcggtcc atgcggattt tgccctgctg atcgccaatc ttataggtca catcataacc
    27181 gagcattttt tctgacttgt catacaccgt tttacaacgc tgttgcgtag tcgtgtaagt
    27241 atcgctttcc tggagagagc cctggatctg gttaccggca tatccaccac ccagcgcccc
    27301 cacaacagtg gcgacatctt taccacgacc accaccaaac tgatgcccta tcacgccgcc
    27361 agcaacagcg ccgagcaccg acccggtaat gcgattttca tcctgcaccg gtcgacgatg
    27421 ggtcactgtg acgttgcgac actcctgacg cggtgtttta accgtttcct tgattggggt
    27481 tgcagaaaca acctgagcgt attgcgggcc ccgttcaaac acgttcagac tggccactgc
    27541 cgctacgccc agcgcagctg cgacaccaat cccgataccc gccaacattg atttattcac
    27601 gggaacacct ccttctttgc agttatgcca atcatgcaag cgaaggagaa aagcagctat
    27661 cagacagaag atgaaaagcg cgggaatggc tggaaatact taaggattcg gagaagtctg
    27721 aaggaatgcc tgatgcgacg cttaacgcgt cttatcaggc ctaccgctcg taccatttgt
    27781 ccagggcgga tgaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca
    27841 aacgcggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt
    27901 aaccatgcag cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc
    27961 gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctaccg acagtggaga
    28021 agttcgacag cgataccagc gaaccatgat ctttatactg ataatttttc agcggtttac
    28081 cgttcatctg cgccagaata ttgttcattg cgcaggtcgc catctggtgt gcagcctgag
    28141 cacgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt
    28201 aaatgtctgg atcgcgggtg gtttgcagcg tcggttccac caccagctgg ttgatacggt
    28261 tggtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccaca
    28321 ccatcagatc agcctcaata tattcaccat ctttagtgtg caggccgcct tcatcagcac
    28381 tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg
    28441 cagcagagat acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta
    28501 cgttcagggc ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcat
    28561 tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca
    28621 ctttaccatt cgcgcccagg ttggcggagt atttcaggaa cagattcagc atctcctggt
    28681 ggaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg acacctggcg
    28741 tattgaaatc gttagaggtg ctacccagcg ccattaccag ggtgtcatag gcgattttac
    28801 gttccggaac cagcagttca cctttctcat cgcgcagttc tgcaatagtg attgttttcg
    28861 cttcacgatc aatatcaatg acggaaccca gctggaactg gaaaccatga ttgcgcgcat
    28921 gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca
    28981 gcagcggttt ccacagatgg ctgtggttac gatcgaccag cgtaattttg gcttttttct
    29041 tgcgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga
    29101 caatcacaat ttttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt
    29161 gttaattaat ggttataaac atagctctta attaacaaca ggttacagca atgaaaatgt
    29221 tcttcgctat tgagaataac atgaatggtg cattggtcat accaaaattg atgttaatca
    29281 agttttgttg ctgaaaagat aggcgacttt accgggtgag atcaaaaaac agagtttatt
    29341 gtttattatt tgagcaagtc cggaagggtg atatagggtg acgcgtatat cgagggcgtt
    29401 tggtcgtcct gataagatgc gcagcatcgc atcaggcatt gatgctcgac tgtcggatgc
    29461 ggcgtaaacg ccttatccga cttacagaat tcggagcgca tcaggtaccg gcaaggttac
    29521 ctgatgcgac gagggcattt acccgagggt tttgaacgcc ttaatgcgct gcaaatgcgg
    29581 ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt aataatgatg
    29641 caactcttca gatgtccgct ggctgttaag cgcttcatca ttacgcgaca aaatcaccag
    29701 gcaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa tatcggcata
    29761 ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat tagggttgaa
    29821 gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc cgcccagacc
    29881 aacgccgcaa atcagcggac gctcgtcgac gttcagttgc aacattttgt ccacttcttt
    29941 gagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca agcgtacatc
    30001 cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt tagagtcaaa
    30061 accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga cctgctttct
    30121 cttcctgcca gcgttcatgt aactgattca gacgggtgct gatcgccttc cagcgagccg
    30181 agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta acacgttctg
    30241 ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta ttgcagacca
    30301 ggatcatatc gcaacctgcg tccagtgatg cctgcccgcg ttcggcataa ctgcccataa
    30361 tcgcggcacc ttccatcgat aaatcgtcag agaaaattac accgtcaaaa cacagttcct
    30421 gacgcaaaac ggttttcagc cagtaggaag aaccgctcgc cggacgcgga tcaacatcac
    30481 tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt aaggaactga
    30541 agaccgacat atctttagcg cgaatctccg cttgtggacg tggatcgcac ggtgtttctt
    30601 tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc gttttcattc
    30661 cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt tgtggatcgg
    30721 catgataaga acgctcgcca atcgccgcgc tgatatgccc gacatccagc actggcgcaa
    30781 agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc gcctcttgcg
    30841 ccagtttgcc gccctcttcc attcccaaca gcgcagcaaa ggattgtgct gccggtaagc
    30901 gggtaaaacc ttcgcgaaaa cgctgcacgc gtccaccttc ctgatctacc gccaccacca
    30961 gatgattgcg cgatgctgcg cggatctggc gcaccagttc acgtaactgg gcaggatcat
    31021 gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgccagt atttcacgct
    31081 cttccgcgtc cagttcgtaa ccttcgacat ccaacattac tggacccaca ctgctctcct
    31141 tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa ttgttgatcg
    31201 ccgatttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag ccagggaaac
    31261 catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt ggcatagtca
    31321 ttgaccaatt gccggtgctg ttcagtattt tccacccaca ccgccgccag ttccagcgcg
    31381 atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc gctatgcact
    31441 aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg tggttcccgc
    31501 gctttgcgca gacgttttaa cattcgcagc caacccacag tccgccgcgc cggatcgctt
    31561 tgctgccagt acagttccag taagggcaac agcgttattc gccagccaaa acgtggttgt
    31621 tgatgaagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata cgtttttacc
    31681 gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg cttcggtgca
    31741 atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga ctgcggcgca
    31801 tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat gagaaaacta
    31861 ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa aaagcgcgac
    31921 agcaattcgt cgcgcgtgat gggattattg ctgcgaaacg gcacctttac ctgaccagat
    31981 aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt taacgttgcc
    32041 agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc ctatggcttt
    32101 actacgggta cctaaactgt cctgcggcga caaacctaac tgttgcttag ccatcgacag
    32161 ctgctgggcg gaaaccaggg taaatttccc gttattagcc agcgcatttc gcagcgtttc
    32221 ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat caaccagcag
    32281 gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg gctgcattgc
    32341 gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgtttgat cttcgtgctc
    32401 aattgggcct ggctgctgcg ggatcgtcgg caccgagggc actgtgggga caggctgttg
    32461 tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag gttcacgttg
    32521 ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc gactcatttt
    32581 tgtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc gcccccagaa
    32641 aattggcgct gccgtacagc gttaccgccg aatgtgcggg aatgctaacg ctgcgtggcc
    32701 tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg
    32761 taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg
    32821 tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca
    32881 gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca
    32941 acgccagaga caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag
    33001 cattggtccc agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc
    33061 gccatggcgg ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc
    33121 agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac
    33181 gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg
    33241 cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc
    33301 acgacgaata attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata
    33361 gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta
    33421 aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta
    33481 tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg
    33541 tttacacgac aaatatgaat gcgcatattt ctcatttgca tttacaaaca aaattattcg
    33601 cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac aacataaacc
    33661 aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca agccatcacc
    33721 aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc cgcttttgct
    33781 gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc agatgatggc
    33841 gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat gactcaactt
    33901 gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca gcagttacaa
    33961 acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc ggattccgat
    34021 cgtgctcttt attattcccg tggattccag atcgataact atatggttga tggtatcccc
    34081 acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc actctttgaa
    34141 cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa tccatctgcg
    34201 gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga tgtctcggcg
    34261 gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc actcaccgaa
    34321 gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc atggctggac
    34381 cgctacaaca gtgaaaagac cttcttctcg ggcattgtcg atgctgattt aggcgatctt
    34441 acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc aacctggggc
    34501 ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgtgc acgcagtacc
    34561 gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac cctgaagcag
    34621 cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt cgaatttgac
    34681 agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct ggttgggcca
    34741 tacagtaatt atggacctgg ctttgattat gtcggcggca ccggttggaa cagtggcaaa
    34801 cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt tggtcgtcag
    34861 cacaatctag tgtttggtgg cagttacagc aaacaaaaca atcgttactt cagttcatgg
    34921 gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa tttcccacaa
    34981 accgactggt caccacagag cctggcacag gacgatacca cacatatgaa atcgttatat
    35041 gctgctactc gagtcaccct tgccgatccg ctgcatctga tcctcggcgc acgttatacc
    35101 aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac gccttacgct
    35161 ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac ctctattttc
    35221 cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac cggtaacaac
    35281 tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac gttagccatc
    35341 ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc cggcagcaac
    35401 ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga atttgaactc
    35461 aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta tattgcagag
    35521 gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa aatgttcacc
    35581 agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa ctggcaaaat
    35641 cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca aggtagctac
    35701 gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt acaggggaac
    35761 gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat cgtctacggc
    35821 gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc tgtaaaaaag
    35881 gcagccatct ggcggcctta gtctccccaa cgtcttacgg attagtggtt acggatgtac
    35941 tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg aacaccagaa
    36001 ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc cactttagac
    36061 acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt aatgttttct
    36121 ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt tgcttttgca
    36181 tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat cagcacgcgg
    36241 aagatggtgt agtaaacaat cgcataaccg ataccgacga tcgggaacag ccacagtttg
    36301 ctgctgttac cagacagaac gataaagtcg atcagaccgt gcgagaacga cgtaccgtca
    36361 cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc gtggatgatg
    36421 tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat accggtcagg
    36481 aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt ttctggttta
    36541 gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa caggaagcca
    36601 ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc gccgtggaaa
    36661 acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac gttccagatg
    36721 tggtgcagac caaacggcac caggcaacgt tcgatgaaac cgtaaatgcc aaacgcaact
    36781 accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc aatcggcggc
    36841 caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat gatcggcaca
    36901 aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta gaaacggtta
    36961 aacatgtacg ctgcgatcgc accggagata atccctccga gtacgccagt atccgccagg
    37021 tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac cacggccatg
    37081 gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc atcgttattg
    37141 gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa gacggaaccg
    37201 cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt cgcggaaccg
    37261 acgcccagca gaatacctgc gataggcagt acggataccg gcagcatcag cgatttaccg
    37321 accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc tgagtatggg
    37381 tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt acagggcatc
    37441 taagcgccct ttatttatta cacagagtaa aataattcag tgccgatttg tttgacggct
    37501 atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac atatgtcaca
    37561 atatttcaaa cgtggttatt caccgcccaa ttttcggcgg tgaactttac tcgttttagc
    37621 cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg ggaagcgtcg
    37681 atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc aacggcaaca
    37741 cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg attctctttt
    37801 cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa ccgatccagg
    37861 gggacataac gcgcagcatc tcgaagttgc tccgcattac ggaaggtcac aatgccggaa
    37921 aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc ctctgtaaaa
    37981 cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc cagcgtatcg
    38041 gcgcgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc aatctggata
    38101 tgatggatga aggactcttg ctgacgaact ttagtttccg gtgtgtaata ataatccagc
    38161 ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa atcttctaca
    38221 tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac gttgtcacgt
    38281 tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac tgccagacaa
    38341 aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt atgcaaagat
    38401 tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat gtctctctta
    38461 aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac gcagtaaaag
    38521 atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt cacgaatgtg
    38581 gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg ccagttcgac
    38641 gacaaggccc ggcacatcaa cattggtcac ctgcgcagca ccatgatggc gttttagcgc
    38701 atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat gattaagggc
    38761 cgccagcagc gaataccagt cgcccgatgg cacgctatat gccaatgcct gacacaatgt
    38821 ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag gcgaaccggc
    38881 gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc gtgaaagcca
    38941 ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac gactacgtaa
    39001 tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt ctgctggtgg
    39061 ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg cagcatcggt
    39121 tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt cggtgacctc
    39181 acgtactgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg tgtagtaatc
    39241 gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac ttttgtggcc
    39301 ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat catcgcccat
    39361 gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat agctggctac
    39421 cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa ctccttcacc
    39481 cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg ggtggcatca
    39541 atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg ggtgcgatta
    39601 aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg cgcgcgtttt
    39661 aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa atccccgaga
    39721 acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc gccctgatac
    39781 gcctgagtgg agagatcgtg gcgatcgcca atcacccagg tgccgttagc cagagctggt
    39841 ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac ttcggcttta
    39901 tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct taacttttcg
    39961 gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc cagttgctca
    40021 agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag cccctcaatg
    40081 acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct gcacagactt
    40141 gttatggctg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg ccacaaaata
    40201 gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc ccggcgtagc
    40261 tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg gcgtttccag
    40321 gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca cggttgggtc
    40381 ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa cctgatcgcg
    40441 ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca gctgattttt
    40501 atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg ctttcaccat
    40561 tttcttgtgc gctcgcttga gtaacgcgac atcggtggta ttggcggtat acatccaggt
    40621 gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg cctgcgctac
    40681 ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac gcaattgctt
    40741 gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg cttctttacc
    40801 gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc ggtaagtccc
    40861 ggctttaaag tgagaaagat ccggttcgat acgcagcaac cattgaaaaa cccgtgggcg
    40921 attgatgatc ttatcggcat aaagctgttc accaagcgcc agacgtccgg tccctggctt
    40981 cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat ggcgaacctt
    41041 ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga taatcaataa
    41101 cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc atataacgtt
    41161 gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat taacgcatta
    41221 caaataacca tctcatctgc ctgcaacgcc tcttccagag aggcttgcac ttcgacaagc
    41281 tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc gttaacacct
    41341 gcctgatcca gacgcggcgt ataaactaca ttgcccttcc gccagaacaa attagccgca
    41401 cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc gtttgtctgc
    41461 tcaagatgag agcggatcaa tacttgctca agccgattaa gatgtttaat acctgcaaga
    41521 tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc gttacgcaaa
    41581 cggtcgtaat gtgcaggata aaccgtaacg gagagaatcc gcgttgccgg tccactgttc
    41641 aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag cacaccattt
    41701 tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa gtcacagaaa
    41761 atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa caaactgact
    41821 ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt tgcccgatcg
    41881 cttactgcca gcgattcctg cttatgaccg ttaattaaga acataccggc tccttatcag
    41941 agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa gctaagaaaa
    42001 aaggcccgca agcggacctt ttatatgggt gggaaatgac aacttagatc tttttaaaga
    42061 tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat tccattccgc
    42121 tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc gggttatcca
    42181 ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag atagattcta
    42241 ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt accaacacac
    42301 ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta tcgccagccg
    42361 gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg cctgcatcac
    42421 gcagagcatt tgccatcgcc agagctgcgc ctgcgccatt ttctggcggt gacgtcatat
    42481 gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc gcaccgcgtt
    42541 ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc agtacgaagc
    42601 catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg cgggtagata
    42661 atgcacgtgc cgcgccaaaa ccaccaacac ccagcggcgt actggctttc tctgcgccac
    42721 ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatgg ccaatgttgt
    42781 gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg ccatacatga
    42841 tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat gggctgatct
    42901 tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg aggccgccaa
    42961 tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttata tcaaggccag
    43021 aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca tccatcttgc
    43081 gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag ccagcaaatt
    43141 tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc tgaccggcaa
    43201 gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg cccagtccgg
    43261 tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa tgattctagt
    43321 gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct
    43381 ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt
    43441 ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag
    43501 aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc
    43561 ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt
    43621 tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc
    43681 aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat
    43741 aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc gcaacatgag
    43801 cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac
    43861 ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg
    43921 cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca
    43981 gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc
    44041 gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag
    44101 cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac
    44161 gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg
    44221 aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat
    44281 cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt
    44341 tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt
    44401 tggcacctaa ataatcgctg atcgcctgag cgccattttc actggtcgca gtgccaataa
    44461 ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac
    44521 cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcaagcg
    44581 ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt
    44641 tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg
    44701 ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg
    44761 cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa
    44821 ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt
    44881 tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagagcacgt tttgcgcccg
    44941 cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct
    45001 gtcccggaga gttaaagttt accggagaaa cgacctgacc tgctgcagct tcttcacacg
    45061 ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcgccc gtaccttccg
    45121 gtacggcttc ttgcatgagc ttgccgcgca tctcaaccag acgcaccgca tcagcgaaat
    45181 caatcacacc agcgcaaacc agcgcggaat attcccccag gctgtgaccg gccatcattg
    45241 ccggtgcttt accgccctgc tgctgccata cgcggtacag cgcaacagag gcagtcaaca
    45301 gcgctggctg ggtttgccag gttttattca gttcttcagc tggcccctgc tgggtcagcg
    45361 cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat
    45421 agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa
    45481 atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg gagccccagg
    45541 tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc
    45601 gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca
    45661 gcgtcaccac gacattgtcc atagacatac cgagtttttt cgccgttgca ctgataatac
    45721 gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga ttatttgccg
    45781 ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc
    45841 ccgccatcgt cagatgaatt gaattctctg gattcacacg gtcagcatta ggcagcgtca
    45901 gcaactcacc atagctaccg tcggcatgca gatgggtgga gatgattccc ggctcttcag
    45961 aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatcgg
    46021 ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc
    46081 cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg caggctgctg
    46141 caacgtcaaa tgccgggcaa cctttaatgc ccagcatgct ttgaatctga caagctgcgc
    46201 tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttat
    46261 caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt
    46321 cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag
    46381 aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc
    46441 cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac
    46501 ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg
    46561 tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga
    46621 acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg
    46681 gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac
    46741 tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat
    46801 aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac
    46861 atcctgacaa caccttccat cgtctttaat gtgacatttc ctgtaaagcc gtcacaaacc
    46921 agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata
    46981 gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc ctttacttct
    47041 tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc
    47101 agaactgagc ccataatggc aaattgcacc aacattgtac tgtcacaatc gacgttggcc
    47161 cctaagtcaa ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc
    47221 ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg
    47281 gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccacg
    47341 cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc actggcgata
    47401 actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt
    47461 aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc
    47521 agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct
    47581 aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg
    47641 cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct
    47701 tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag
    47761 ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac
    47821 gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt
    47881 ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa
    47941 atgggtttgg cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca
    48001 cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt
    48061 caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt
    48121 ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga
    48181 cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat
    48241 cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat
    48301 cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac
    48361 cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta
    48421 ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag
    48481 cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt
    48541 cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat
    48601 caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc
    48661 atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag caccagaggt
    48721 cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga
    48781 aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt
    48841 atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cctgtctgca
    48901 attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc
    48961 ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctgag
    49021 cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt
    49081 taagagtgaa ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac
    49141 gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc
    49201 gctgatggga tagttgtttg cgctggatgc ggttacgtcg catccggcaa tcggtgcagg
    49261 cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct
    49321 tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg
    49381 cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca
    49441 ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac
    49501 cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat
    49561 gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca
    49621 ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc
    49681 tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac
    49741 cgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag
    49801 aacgcaacgc cgagcgtttt ttcgctatca gcaacacacc tgaagtgtcc cggtcgagac
    49861 gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc
    49921 cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga
    49981 tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg
    50041 aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg
    50101 cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttcg cctttacgca
    50161 aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga
    50221 tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt
    50281 tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat
    50341 atttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca
    50401 atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct
    50461 tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa
    50521 caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta
    50581 agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg
    50641 gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc
    50701 atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg
    50761 cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag
    50821 cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca
    50881 actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtcttta tgacctggat
    50941 atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc
    51001 attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc
    51061 ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc
    51121 aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag
    51181 cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt
    51241 ctgatgccaa ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt
    51301 accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg
    51361 cgcaccgctg gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg
    51421 aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat
    51481 caggagagca acgtaatcgt tcgcgcattc cgcgattact tacgtcagga catcggcgaa
    51541 atccttatcg ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc tgcattaggt
    51601 cgcccggatt tcagcagcaa aatcaaactg tataccggcg agatcccgct gttcagccac
    51661 taccagattg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccgtccggt
    51721 ggttcgattg ttatcgacag caccgaagcg ttaacggcca tcgacatcaa ctccgcacgc
    51781 gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca ccaacctcga agctgccgat
    51841 gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc
    51901 gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt
    51961 caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc
    52021 cgccagcgcc tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc
    52081 ggtaccggta ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa
    52141 gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct
    52201 tcttatctgc tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt
    52261 gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc
    52321 gtgcgcaaag gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa
    52381 gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca
    52441 ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa ccccagctga acctgccgcg
    52501 actgtcgtag ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct
    52561 gggctgttaa gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc
    52621 aaaccgaccg agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt
    52681 cgcaaacctc gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa
    52741 cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag
    52801 cagactgccg agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc
    52861 accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt
    52921 caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa
    52981 caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa
    53041 gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact
    53101 gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc
    53161 ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac
    53221 cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt
    53281 ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg
    53341 accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt
    53401 gtacgtccgc aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag
    53461 ccgatggtga ctggggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcaccggtt
    53521 gttgaagaag tggctgaagt tgtagaagcc cccgttcaga ttgccgaacc gcaaccggaa
    53581 gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcag taactgaaca gccgcaggtg
    53641 attaccgagt ctgatgttgc cgtagcccag gaagttgcag aacacgcaga accggtggtt
    53701 gaaccgcagg aagagacggc agatattgaa gaagttgccg aaactgctga ggttgtggtt
    53761 gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt cgcagcagaa
    53821 gttgaaacgg tagctgcggt tgaacctgag gtcaccgttg agcataacca cgctacggtg
    53881 ccgatgacgc gcgctccggc accggaatat gttccggatg caccgcgtca cagtgactgg
    53941 cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac ggcaacacat
    54001 catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt aataattagc tcaagtaatc
    54061 aagccctggt aactgccagg gcttttttat ttcatctttg aatcaacata ttaaaattca
    54121 tcgaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc cgccccggcg
    54181 atcccagagc aggcagacaa aacataccca gtttcatgat atgttttaaa gcgaaattat
    54241 ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata cgatgcctgc
    54301 aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac atcaaccaga
    54361 tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc cagcgactcc
    54421 agttcgttca gctgcgtgcc taattccgcg cgaacggtca gcacattgtt cagcgagttt
    54481 ttcagtccgc ggttggtttt atctaacgcc gcagcggcgg tttctttatc cgcttcgcta
    54541 tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa aagattggtt
    54601 tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc gaaaattttg
    54661 tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt aatactttct
    54721 gttccgccga cataatcacc gttagcctca ctaaacggcg cagtctctgt tttataaccg
    54781 gcaaaaatgt agcgcccatt accgtcagtg gtgttcgcca gattcagcaa ctggtcacga
    54841 agcccctgaa tatccgtagc cagcgaggcc cggtcatcgt cactcaaggt gccattgctg
    54901 gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct aagtacactc
    54961 tcttccagtg acactttttg agtggcgaaa gtacgcgcca gcgtgtactg gctgttttgc
    55021 gcctgtgcct gggagagaac aaccgcttgt gatgcagcaa tgggatcatc tgaagggtta
    55081 acgactcgct tacccgtcga catctgttcg ccgtacttca tccattctgc ctgagaattg
    55141 gtgataccac gcatgttttg ctggtacatc atctgggtac tgaaacgcat tttatctccc
    55201 cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat
    55261 tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg agattgacac
    55321 cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc gtggtgctac
    55381 tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca taagcgtcgt
    55441 taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc agggcctgac
    55501 cgttgcggtt atcgctatca cccgcatctt tttcgctcgc catcgctatt ttcgcttcgt
    55561 cggtgattaa tacatccatg ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt
    55621 cgttaacggc aggcgttcct gtaaacgtca actccagacc atcaaatgcc actttaccgt
    55681 tggcatccgg cgtcaccgta aaagtggtat tgctggcaag gcgggtgacc tgccactgat
    55741 tattatcgaa cgagattttg taatctgtcg ccagtaccac ggaggcatca gttaccgtgg
    55801 caccgatcgc aacgtcacct ttgtttttcg tgttttgcag aaccgcgggc ttaccgatag
    55861 taaagaaatc ttcaccagca tcgccgttag catcaaatcc ggctttgtgt tgggtgttga
    55921 aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg tccagctcct
    55981 gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc tccgggatct
    56041 caatattgcc tgccgtccca tcaacataag cgacagtcgt acgagaaggg tcagcgctgg
    56101 aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa ccattggcca
    56161 ccgtgatgtt ataagtgccg ccatcctgaa cgctgacttc tacaccaaca atctggttta
    56221 attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc cctgccccca
    56281 cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat ttgtttagcg taattgttga
    56341 tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg cgcagatatt
    56401 gatcggtggt tttaaactga ttcaccaacc cttctgattt cccaatcagc gcctggcgcg
    56461 ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct
    56521 gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac atctgctcat
    56581 agcgggcggt caggccgcta ctttgcgtct gcgcagcacg taactggttg gtaataaacg
    56641 catcatactc acgctgcaca ccagaaacgt agacgccatt gccaacccag ccgccagcgc
    56701 ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg gcaacgttat
    56761 agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc agtccgctca
    56821 tggcgttatt gatcaagctg gacatggagg ttccttataa gccttcaatt acagagtatt
    56881 atcggcagcg acccgccgga cttgagttat tcagaacaga ttatcaatgt tcatgctgta
    56941 ggttttgctc accttgtcgc ttatcgattt catctgctgg atcatgttgg tgagtttacg
    57001 ggcatagtga ggatcggtag cataccccgc gtcctgtagg gcctgcgccc cctgctccgc
    57061 actcgcggct gtcgtcacgg cggcgtagcg cgggttacgt gttaacagcc caacgtaatc
    57121 cgacaatgct tccagatacg agctgtagac ccgaaatttc gcttttactt tcttcgcttc
    57181 gccattttca tattcagtcg tggtgatttc agtaactgga cctttccagt tgccagaggc
    57241 tttgacgcca aacaggttat agctcggctc gccgttttcg cggcggattt gccgttgccc
    57301 ccaaccagac tccagcgccg cctgagcgag gatcaaatga tgtggcacac cgctttgctg
    57361 gcttgccagt tgggcgggca gcgagagttg cgcgaggaat gctttactgt cacccggcag
    57421 cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg
    57481 ataacgcacc acagtttcga gcgggaattt catcggtgct gctggcgtgg actcctctgg
    57541 caatggttgt tctggcgtca tctgtttaac catcatctct gcaagcccca gacctttgcc
    57601 cgccgtcatc tgttgggcaa tctgctggtc atacatactg gtatacaggc gagtgtgctc
    57661 gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac
    57721 gaacatccct tccacctgac gggccaccgg acggatattt gccgccggat cttcgcccgc
    57781 tttcgccttt agttcgttga gtgattgcgc atcccaggcc gcacttgcca gtagtttgct
    57841 gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact ttgcattgat
    57901 tgcagtatgg acatcagatc catcggcgta gcgcccagcg cattgagcgc gcgcaccacg
    57961 ttattgaggc tggcgctgga acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg
    58021 atctgcgttt gtggagtaac cacagtctgt ccaccaccaa acggtgtatc tggctggctg
    58081 acattggcct gacgattgac tgttactgag agatttccct gcgctaccgc gcagctgtcg
    58141 agggtcactt cgcgattcat caccaccgaa ccggtgcgcg agttaatcac tactttagcg
    58201 tcctgcgggg tgacattaac ctgcatattc tggatatctg caaggaaacg gacctgggaa
    58261 ctgttgccac ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca
    58321 tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg
    58381 ttaagttgca aattaagggt attcccgacg ccaaactggc tgggcaattc acgttcaata
    58441 accgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact gctaccgcca
    58501 gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata cacctgactg
    58561 tcaacgccct taagcggcgt catcaacaac gtacctccac gcaagctttt ggcatttccc
    58621 atggaagaaa ccaccacatc gatggtttgc ccctgacgtc caaacggagg aagtgactct
    58681 gtcaccatta ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg aacggtaatt
    58741 cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg
    58801 tcaccggtgc catccagccc caccaccaga ccatagccaa tcagtgagtt ttgccttacc
    58861 ccctgaacac tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt aaccagtaga
    58921 agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc gacaggttaa
    58981 ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt
    59041 attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg ctgatagtgc
    59101 gtggattaac cacgccagag aagcgaataa attcggtacc ctgattaatg gcaatctgtt
    59161 tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg
    59221 tgccgctaaa ggtattgctg gcattggccc cgccctttcc gttgaacgtg ttaccaccgg
    59281 aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc ggcacagtat
    59341 caaagccaaa attagtttta ccgtcacggc tggcattcgc agaggagctt ttgctggcgc
    59401 tgacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt cgacgatctt
    59461 caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata gaaccgttgg
    59521 cgacgggcgt cggaccggga accggttgtg cactggtcgc cccctgcacc agcggcgtgg
    59581 agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg gcataagtat
    59641 gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca gtaaaccggt gaacctgcca
    59701 ccggttaagc cttagagttg cgtcagtttt tgcagcatct gatcggtggt ggacaccgct
    59761 ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc ttccgccacg
    59821 ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt caggcccggc
    59881 gtgctttcgt tcggtacacc agaggattgc gtttcggtgt agaggttttc gccaatgctc
    59941 tccagcccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg aaccggagct
    60001 gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat acttaacgca
    60061 ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagctgcccg ttctgatcca
    60121 cctggaaaga gccgtcacgg gtataggctg atgaaccatc tggcaacatc acctggaaaa
    60181 agccctgccc tttaatcgcg acatctttgc tgttgttggt ctgcgacagg tttccctggc
    60241 tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc gatttgtaat ccggagggta
    60301 aggtggtttg ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt
    60361 caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa
    60421 tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga
    60481 tcataaaatg tcctgtagtg gattagtgac aaagtgcata tgtcttcttg ccggatgcgg
    60541 cgtaaacgcc ttatccggcc tacaagtgct cgcaaaatcg ataaattgcg attgccccca
    60601 tagacccgat aaacttgcgc atcgggcaac ttatacttgt catgtatcct ttcaattaac
    60661 tcatcgacag cagttggttg gcacggcctg cgttatcatc gacgctgctg atcaccttca
    60721 tctgcatttc aaaacgccgc gcgctggcaa tcatgtcgct cattgccgca acggcattga
    60781 cgttactgcc ttccagaacc cccgacatca cacgcaaggt tggatctgcc tgcagtaccg
    60841 gccctcgcgt ggcctgggtt tctgcgctta aacgaaaaat gccgtcgtca ccgcgctgca
    60901 cttcgctgcc cgtggctttc accagtttca gacgccctac tggcgcaact gtatttgccg
    60961 gatcgcccgg attgagcgcc gagattgtgc catcggcagc aatagtgatt tccgctcctt
    61021 ccggcacagc aattggcccc gcctcgccta tcacctgatg cccctgaatt gtcagttgcc
    61081 cggtgggatc aacctgaatg ctgccattac gcgtataccc ttcgctgccg tcagcggtct
    61141 gcacggccag ccagccatcc tgctgcaacg cgacgtccag cgggcgcgag gtgtaatcca
    61201 ttttgccggg cgtcatatct gcgcccggcg ttgacgccgt gaccaacgtg cgcgtgggca
    61261 gagaaagccc ttccactggc accgcgcgta aagcattcaa ctgcgcgcga aaaccgggcg
    61321 ttgaggcatt ggccagatta ctggcggtta ccgcctgttg attcagtgtc tggctggctg
    61381 ctcccatcgc ggtataaatt gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag
    61441 ttaaccagcg tgttgaggat ctggtcctga gttttgatgg tctgggcgtt agactgatag
    61501 ttacgctggg caacgatcat attgaccagt tctttactga gatcgacatt ggacgcttcc
    61561 aacgcaccgt tggtcagggt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg
    61621 ccagaagatt gcgtcgcaga ccagacgttg tcgccttcgg atgccagacc ttcgttgttg
    61681 gcaaagttcg ccagtacaat ctgccccagc agttgtgttt gttcgttgga atagttgccg
    61741 acaaccgtac cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccattc
    61801 tgggtggttg ccacaatatt gttagcgccg gtattttgct gcatggagtt gaggaagctc
    61861 aggctaaacg tggcgggatc tgcgccgtta attgcgccag tggtaatatt ctctgttgga
    61921 tttgaggtca gaacgccatt ggcattaaac accagcttca ttgcaggctc ggctgtacct
    61981 gttggatcac tgctatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaag
    62041 tagacgctca tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg
    62101 ttatagctat ccgcattgct ggcgctgaat ggcgtaacag taggaagcgg atcactggaa
    62161 ttcaggttga tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg
    62221 atcgaaatat tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg
    62281 taacccgtca gctgtaaacc ttgcatattc accaggttac ggttttcatc cagcttaaat
    62341 tgtccgttac ggctgtagaa caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc
    62401 tggctgatag caacgtccag acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc
    62461 tgagtgatac cggcaacttt tacccccagt cccactttcg aaccggcaaa catatcggca
    62521 aaagaggccg tgcctgattt aaagccgtag gtggcggagt tggcgatatt gttgccaata
    62581 acatcgaggt tggtggcggc agcgtttaat ccgctaaccg cttgagaaaa ggccatgact
    62641 gactcctgaa atgtgaaggc ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg
    62701 taagtgccga gatccagcgt attaccgctg ttgccgcgga tcacaccctg caccagagca
    62761 aactgcagcg gctgggcaac cagttgtgta ccaccgttac tggcgctaat cgctacattg
    62821 taagaaccgt tcggcgcagt gctgccatca gtcaacgtac cgtcccaggt gaaactgtgg
    62881 actccggcgg tcagttcacc aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg
    62941 atggtggccg taactttgtc tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg
    63001 gtcacagccc cttcttcact gccggttccc gcaagaacag tggtgccggg gatcatcacg
    63061 ccgtgaccga tcaggttact ggcctgtaac gactggctgt tatcaatctg tccggaaata
    63121 gatccgagcg tggtattgag tttttcaatc ccgctgaccg tgctgatttg tgccaattgc
    63181 gacgtcagct cgttgttttc cattggattg gtcgggtcct ggtttttcag ctgcgccacc
    63241 aacaaagtca gaaaactgct ttgtaagtct gcggcgttgc tgcccgtgag cgaactactg
    63301 ctggtggtac tgacgccggt atttgtcgga tcggtggtgg ttaccgcaat ggacatagct
    63361 ttctccttta ttgaccgagc gtaagggttt tcagcatcat gcttttcacc atgttgagca
    63421 cttcaacatt ggcctgatag ctgcgtgacg ccgacatggt gttaaccatc tccccgacga
    63481 catcaacgtt cggcattttt acgtagccct ttgcatccgc cagcggatta cccggttcat
    63541 aaaccagttt gtccggggcc tgactttcta taacatcggc aacctttacg ccgcctgtcg
    63601 cagcacctgg tgcagcgtta acctggaata ccacctgttt tgcccgatat ggctgtccat
    63661 cgggaccggt cacgctatca gcattcgcca gattactggc cgccacgttc aagcgctggg
    63721 actgggcagt taacgccgac ccggcgatat caaaaatatt cagcagtgcc atccgttaat
    63781 ttccgctctg taaaacgttc atcatgcctt tgatttgccc gctcaacgcg ctaaggctca
    63841 tctggtattg caggctgtta tcggcaaact gggtgcgttc gcgatccata tcgacggtat
    63901 taccgtcaag cgaaggctgg tccggaatac ggtattgcag ttctgcggta ggaggcgtca
    63961 gcgcctgcgc cggaatgtgt tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat
    64021 cccgtccacg ttgcatgact tttttaagtt cactggcaaa atcgatatcg cgcgcctgat
    64081 aaccaggggt atcggcattg gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca
    64141 gattgagcgc ctcttgttga aaacgtaagg cggcgtcgag cttatcgagc atatctcctc
    64201 cgcaggtatc aaaattctgc catcagctta aatgccttta cgcgcgcgtt atcggcggaa
    64261 taaacgcaaa atgggtcgct atttatgccg ttgatgacca ttgcggacag gtacaattca
    64321 cgttgtagaa atggctgggg gcgaaaatgc tggcaataaa acgtagcgtg gcgatcatct
    64381 cgatactatt cactccgtta agtgcggcga gtaatctcac atcgcaattg caaacctttt
    64441 ttagcgccca gctcgcgggg gtaagtgatg aggttcgtgt ttctattcgt acagcgccca
    64501 atctactacc gccgtgcgag cagccattgc tttcgatgag caataattcc cgcctgtggg
    64561 gcaatgtgaa tgtgttggca cgctgcggta acgacaaacg atatttacag gttaatgtac
    64621 aggccacagg aaattatgtg gttgccgcga tgcccattgt gcggggagga aagctggaag
    64681 ctggcaatgt caaactaaaa cgcggacggc aggataccct gccaccgcgt acggtgctgg
    64741 atatcaatca acttgttgat gccgttagcc tgcgcgatct atcacccgat caacctatcc
    64801 agttaaccca gtttcgccag gcatggcggg taaaagcggg acaacgcgtc aacgtgatcg
    64861 ccagcggtaa cgggtttagc gccaacgcag aaggtcaggc gctgaacaat gcagccgtca
    64921 cacagaatgc gcgggtgcgc atggtatcgg gactggtggt cagcggcgtt gttgatgcag
    64981 atgggaatat tcttataaac cagtaagctg ttaaagatta cccgtccctt gccgataaat
    65041 aagcaacaca tgataaaagc gccctcaatg aggaataaac catgagtatt gatcgcactt
    65101 cgcctctgaa gcccgtaagc accgttcaac cacgcgaaac cactgacgcg tcggtaacga
    65161 acacccgggc ggcaaaaaca acagcctcca ccagcaccag tgtgacgtta agcgacgcgc
    65221 aagcaaaact gatgcaaccc ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa
    65281 aactggcgat tcgtaacggt gaactaaaaa tggacaccgg caaaattgcc gatgcgctga
    65341 tcaacgaagc gcagcaagac ttgcagagta actgatcgta tgacacgtct tgcagagatc
    65401 ctcgaccaga tgtccgctgt gcttaacgat ctcaaaacgg taatggatca agaacagcaa
    65461 catctctcta tggggcagat caacggcagc cagttgcaat ggattacaga acaaaaaagc
    65521 tcactgctgg cgacgctgga ttacctcgaa cagttacgca ggaaagaacc caatacagca
    65581 aatagcgttg atattagtca acgctggcag gaaattactg tgaaaacgca gcaactacgc
    65641 caaatgaatc aacataacgg ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg
    65701 ctggaaatgt tgaaaccgca tcaggaaccg acgctatatg gggcgaacgg tcagacctca
    65761 acaacccatc gcggcggtaa aaagatttcg atctgaatat tatctccggc ctgcactgca
    65821 ggccggaatg cattgttaca ccgtccggcg ggcaaattct ttaactttga agcccagtac
    65881 cgccagtgca gcgaagtacg cggcaatccc cgccagcacg accgccatta aacgcagtaa
    65941 gcgccaaggc atggtaccca atgaccactc cggcatgata tgtaacatac ctaaaagcac
    66001 gccagacatc accagtaccg ccaccaccag acgcaacaga aacgccatcc agccgggttg
    66061 cggagtaaag attttctgct tacgcaactg ccagtaaagc agcgaagcat tcagacacgc
    66121 cgccagacca atagaaagtg acagcccggc atgtttcaac ggaccaataa acgccaggtt
    66181 catcaattgc gttaaaatca gcgtaacgat ggcaattttc actggcgttt taatgtcctg
    66241 gcgggaataa aagccaggag ccaacacttt cactacaatc aggccaatca aacccaccga
    66301 gtaggcaatt aacgcccgct gggtcatcag cgcatcaaac gcggtaaatt taccgtactg
    66361 gaacagcgaa acggttaacg gacccgaaag aatgcccaac gcaaccgcac tcggcagcgc
    66421 caacaggaaa caaagacgca acccccagtc catcaaacgg ttgtattcat catgattgcc
    66481 actggcaaaa cttttcgaca gcgacggcag caaaatggtg ccaagcgcca cgcccagcac
    66541 accggaggga aactccatta agcggtcggc gtaatacatc caagacaccg aaccggaagc
    66601 aagaaacgag gcaaaaatgg tgttgatgat taaggagatc tggctcacag agacgccaag
    66661 gatcgccggt cccatctgtt tcaccacgcg cattgctccg gcatcgtgga agttaatgcg
    66721 cggcaggacc agcatgccga tcttcttcag gtgcggtagc tgatacacca gctgcaggat
    66781 gccgccgacc gtaacagccc acgccagcgc cagcaccggt gggttaaagt acggtgcggc
    66841 aaacagcgcg aaaccaatca tgctgatgtt aagcagtgtt ggcgcaaacg ccggaatcga
    66901 gaagcggttc cacgtattca gaatcgctcc caccagcgac gccagggaga tcagcaagat
    66961 atagggaaag gtaatcttta gtagctggct ggtcagggca aatttgtcag ctgtgtcagc
    67021 gaagcctggc gcggtcacca tgatcaccca cggtgcggcg agcatgccag cgaccgtcac
    67081 aaccgccagc gcaagtgtca gcaggccaga aacataagag acaaagaccc gcgtggcgtc
    67141 ttcaccctgc ttacttttat attccgccag aatcggtaca aatgcctggg aaaatgcccc
    67201 ttcggcaaag atacggcgta acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt
    67261 tgccatccct gcgccaaaga ttctggcgac aattgcgtct cgtgcgaagc caagcacacg
    67321 cgaaaacatg gtcatcgagc tgacggcggc cagcgatttt aataaattca tcggtgttct
    67381 aatccagacc cacgggcaaa acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac
    67441 ctggattatg gtgaattgct accgccagat gttacagggt ttattcactc atcgcatcgc
    67501 gccagatctt gtcaacgata cgttgcgcca gcactgcctg ttcgccggcg gtttgcagaa
    67561 ctgtctgatt ttgcacacat tcaatgaagt gccgcgcaca gccgacaaat ccacgttgct
    67621 caagagtgct ctgccaacca ggaatcggtt tatgcactac gccctgcccg cgctcctcac
    67681 gccattcgcg catatccgta atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt
    67741 cacgctgact tccggcacgg cgatgcatac aggtggtgat ctgcaaagga tcggccgaaa
    67801 aatggtgctc ggcaaacagc atttcgccag cgtcgttagt cagtagcgta ccgccatcca
    67861 gagaggcttt gccgcccgac aaccacagcg cggtatccac cacatgcaga taatcatcca
    67921 gcaacgtgaa ataaagatcg tgtggcccga cactattgct acgatgttta tccattctta
    67981 gcgaagctgc ggtagcgagt tgcgttttta actcaccgta gagtggtgcg aaacgacggt
    68041 taaaaccgac catcaacgtc agttttttcc gcgccgccag ttccaccagc cgttcagcat
    68101 cgcgcagatt ttctgccagc ggtttatcga cacagacatg tacacccgca ttgagtaacg
    68161 tactgaccac gtcaaagtgg ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg
    68221 cggcaaggct ggataacgaa tcggcataag gaatgcgcca gctttcacaa attggcaggg
    68281 ctttcgcgcg cgtaggcgac caggctcctt gtaacatcca gtcagacgct gccgccagca
    68341 ccggtaacca cgctttttgc gcaatgccgc ctaatcccac aacgccgata cgtaattttt
    68401 tcactttaat ctcccagatg ggccagcaac gaatcaagac gctgtttcag ttctgccact
    68461 tcgatttcca gggcttcgac gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc
    68521 gttaccgccg gctgatcctc tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt
    68581 ttacccggtt cgcgggccag acgcaccaca aaaggaccat cttcgcgatt tgccagttgt
    68641 tccagcgtcg actccacttc cgccatatcg ctgaattcat acattcgcgc ggcgcggctg
    68701 cgcagttcac ccggcgtctg ggcaccacgc aataacaacg tggtgattaa cgccacttcc
    68761 gctgcgctca gtttcagatc gccaaattct gaattacaaa aacgttgttc atatttggtg
    68821 acccgattac caaagccact caccgtgcgc agataatggc gtttgaccag attatccagt
    68881 tgctcctgca cttcagattc actcagattc atgaccggtt cacggttcgt tttctgatta
    68941 caggccgtga ctacaccatt gactgagagc gggtattgct ccggcgtggt cacctgcttt
    69001 tccagcaagc aaccaatcac gcgagcttca agggcagtaa gttgatattt catgagattc
    69061 tccttagcgg ccgggcgtcc agtctggggt agttaatgcc gtcagtacgt gatcgcgcca
    69121 ttgtccatca atcaacagat agtctttcgc atagccttct ttttcaaaac ccagtcgcgc
    69181 cagtaaatca ccgctgcgtt tattgtgcgg catataatta gccataatgc gatgaatatg
    69241 ttgggtgcgc tgcatataac gaatggctgc ggtcagggct tcaaacatga gtcctttgcc
    69301 ctgccatttt tgcccaatcg aataaccgag atagcaggca tgaaaagagc cacgaacaac
    69361 attggaaaaa ttggcaacgc caataatctc tttttcgtcc gggtcgaata agccaaagta
    69421 gaaagctgaa ccttgtttat gaaattcgtt aatcatcccc agcctggcct gccagcctga
    69481 tggataacag tggctttcgt cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc
    69541 tgcgtaataa tccgcaagac gccaggcatc acgatcatgc accagacgca cgaccagtcg
    69601 gtctgtggtt aagcgcactt ttggcacgtt actgcgatag ccaaacattc tatacctact
    69661 ccttcccgta acgtctttaa tacgctgtaa gtattactat acctgcgcaa ccagaccctg
    69721 tgaaaagtcc aaaatgccat tttctgcatt attcacatat ttgataaaaa ctctctatca
    69781 aagcgacgtt ttgataaaaa aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa
    69841 tgcagaatag aaagtgctca tcttttattt cccgggaggg gaaatgtccc gcgtatcgca
    69901 ggcgaggaac ctgggtaaat atttcctgct catcgataat atgctggtcg tgctggggtt
    69961 ctttgttgtc ttcccgctga tctctatccg cttcgttgat caaatgggct gggccgccgt
    70021 catggtcggt attgctctcg gtctacgcca gtttattcag caaggtctgg gtattttcgg
    70081 cggcgcaatt gccgaccgct ttggtgccaa accgatgatt gttaccggta tgctgatgcg
    70141 cgccgccgga ttcgccacaa tgggtatcgc ccacgaaccg tggctattgt ggttttcatg
    70201 cctgctctcg ggactcggcg gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa
    70261 actaatccgt ccacaacaac gttgtcgttt tttctcgctg ttgatgatgc aggacagtgc
    70321 cggtgcggtc attggcgcat tgttggggag ctggctgttg caatacgact ttcgcctggt
    70381 ctgcgccaca ggggccgttt tgtttgtgct gtgtgcggcg ttcaatgcgt ggttgttacc
    70441 ggcatggaaa ctctccaccg tacgcacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg
    70501 tgacaagcgt tttgtcacct atgtcctgac gctggcgggt tactacatgc tggctgtaca
    70561 agtgatgctg atgctgccaa ttatggtcaa cgacgtggct ggcgcgccct ctgccgttaa
    70621 atggatgtat gccattgaag cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg
    70681 gagtgaaaag cattttcgtc tggaacaccg gttgatggct gggctgttga taatgtcatt
    70741 aagcatgatg ccggtgggca tggtcagcgg tctgcaacaa cttttcaccc tgatttgtct
    70801 gttttatatc gggtcgatca ttgccgagcc tgcgcgtgaa accttaagtg ctttgctggc
    70861 ggacgcaaga gctcgcggca gctatatggg gtttagccgt ctgggtctgg cgattggcgg
    70921 cgctattggt tatatcggtg gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc
    70981 agagcttccg tggatgatgc tgggaattat tggcatcttc actttccttg cgctgggttg
    71041 gcagtttagt cagaaacgcg caacgcgtcg tttgctggaa cgcgacgcct gatttgatac
    71101 tcccccctga ctcctccata ctgaaagtga actgacagcc aaatggagga gtcacgtgaa
    71161 gctatacatt tacgatcact gcccttactg cctcaaagcc cgcatgattt tcggcctgaa
    71221 aaatatcccc gtcgaattac atgttctgct caacgacgac gcagaaacac ccacccggat
    71281 ggtcggtcaa aaacaggttc ccattctgca aaaagatgac agccgctata tgccagaaag
    71341 catggatatc gttcactatg tcgataaact cgacggcaaa ccgttactga ccggcaaacg
    71401 ttcccctgcc attgaagagt ggctgcgcaa ggtcaatggc tacgccaaca aactgctgtt
    71461 gccgcgtttt gccaaatcgg catttgatga gttttctact cccgccgcgc gcaaatattt
    71521 cgtcgacaag aaagaggcca gcgcgggtaa ttttgccgac ctgctggccc actctgacgg
    71581 tctgattaag aatatcagcg atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa
    71641 cgccgtgaat ggcgaacttt cggaagatga tattcagcta tttccgcact gcgtaatctg
    71701 acgctggtag ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg
    71761 aaacagacac aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgcggca
    71821 gcctgtattc gcgggttgcc gccgttttca gttttttctg ctggcgagtt tatcgctggt
    71881 gacacatgct gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa
    71941 caagttttta tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact
    72001 cacccaatac accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt
    72061 ctcaaaagat atcggtttac ccggcgtggc tgacgcccat attgttctga caaacttgat
    72121 cagccaaatt ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga
    72181 catgaactcc ctgttcggta gtcagaaagc gaccatgaaa ctgaagctga aagcgctgcc
    72241 cgtgtttgat aaagaaaaag gtgcgatctt cctgaaagag atggaagtgg tcgatgcgac
    72301 ggtacaaccg gaaaaaatgc aaacggtgat gcaaacgttg cttccctatt tgaaccaggc
    72361 attacgcaat tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagtcaggg
    72421 cgaagcaatg gcgaaaaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat
    72481 tccgtttacg gattaatgac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa
    72541 acgtttccgc ttatcctttg tgtccggcaa aaacattcct tcagccggag catagagatt
    72601 aatgactgca ccatcccagg tattaaagat ccgccgccca gacgactggc accttcacct
    72661 ccgcgatggc gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc
    72721 tatcgtaatg cccaatctgg ctccgcccgt gaccacagtt gaggctgccg tggcgtatcg
    72781 ccaacgtatt cttgacgccg tacctgccgg gcacgatttc accccattga taacctgtta
    72841 tttaacagat tcgctggatc ctaatgagct ggagcgcgga tttaatgaag gcgtgttcac
    72901 cgctgcaaaa ctttacccgg caaacgcaac cactaactcc agccacggcg tgacgtcaat
    72961 tgacgcaatc atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca
    73021 tggtgaagtg acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag
    73081 cgtgatggaa cctctgcgcc agcgcctgac tgcgctgaaa gtcgtttttg agcacatcac
    73141 caccaaagat gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac
    73201 tccgcagcat ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct
    73261 gtattgttta cccatcctca aacgcaatat tcaccaacag gcattgcgtg aactggtcgc
    73321 cagcggtttt aatcgagtat tcctcggtac ggattctgcg ccacatgcac gtcatcgcaa
    73381 agagagcagt tgcggctgcg cgggctgctt caacgcccca accgcgctgg gcagttacgc
    73441 taccgtcttt gaagagatga atgctctgca gcactttgaa gcattctgtt ctgtaaacgg
    73501 cccgcagttc tatgggttgc cggtcaacga cacattcatc gaactggtac gtgaagagca
    73561 acaggttgct gaaagcatcg cactgactga tgacaccctg gtgccattcc tcgccgggga
    73621 aacggtacgc tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt
    73681 taacctgtat aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca
    73741 tagcgaaaac ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc
    73801 gccgtattca gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag
    73861 cgaataattt atcggttatt ggcgcaacaa aagaagataa acagcgcatt agcgaaattc
    73921 tccaggaaac gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt
    73981 ttgtttttgc cgggtcgccc cggctttttt attttactta cgaattttga gtacaaaatt
    74041 tctacacttc ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt
    74101 tattgttcat ataaatctta aaagcagaaa tttatctgtt ccccccttta aaaattcggt
    74161 gaataccctt acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga
    74221 ataacgttgt ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt
    74281 cagactcatc cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg
    74341 ttgcgtttgc actaccagac cccaaataag tccgagcagg aagggactga agttggtcaa
    74401 acgctctggt taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc
    74461 gccaaaattg aatccggtga tttccaggta aacgagtatc ggcgtcatta acatgacatg
    74521 accatccacc caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca
    74581 ttcagttaat tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc
    74641 atcattattg gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgcgccggt
    74701 ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc
    74761 gatacgcgat taattcgcca tgcttatggt gaaggcgaaa agtatgtccc gctggtcctc
    74821 cgcgcgcaaa cgctgtggga tgaactttcc cgccacaacg aagatgatcc catttttgta
    74881 cgctctggtg tcattaacct cggcccggct gactccgcat ttctcgccaa cgtcgcccac
    74941 agcgccgaac agtggcaact caacgttgaa aagctcgatg cgcaagggat tatggcccgc
    75001 tggccagaaa tacgcgtccc ggacaactac atcggcttat ttgagactga ttccggtttt
    75061 ttgcgcagcg aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg
    75121 caactgttca actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa
    75181 acggttgacg gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa
    75241 gacctgctcc cggagcagcc tgtccagccc gtacgtaaag tatttgcctg gtatcaggcc
    75301 gatggccgct atagcgtgaa gaataaattc ccggcgttta ccggtgaact gcccaatggc
    75361 gatcaatatt atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga
    75421 ggccaggtta tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt
    75481 tcggaagcct tcccgttctt gcgcaatgta ttgccgggta tcggttgctg cctgtacggc
    75541 gctgcctgca cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc
    75601 cacgataata cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt
    75661 ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca
    75721 ttcaggcttt cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac
    75781 aggagtattt aatgcgtcgg ctgttgcact atctca
//
LOCUS       DAZPJP010000002        66916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPJP010000002 DAZPJP010000000
VERSION     DAZPJP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.002"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..581
                     /locus_tag="ACHU9J_000076"
                     /pseudo
     CDS             1..581
                     /locus_tag="ACHU9J_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            684..1186
                     /locus_tag="ACHU9J_000077"
                     /pseudo
     CDS             684..1186
                     /locus_tag="ACHU9J_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1253..2710)
                     /gene="nuoN"
                     /locus_tag="ACHU9J_000078"
     CDS             complement(1253..2710)
                     /gene="nuoN"
                     /locus_tag="ACHU9J_000078"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1903745.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2717..4246)
                     /gene="nuoM"
                     /locus_tag="ACHU9J_000079"
     CDS             complement(2717..4246)
                     /gene="nuoM"
                     /locus_tag="ACHU9J_000079"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1903746.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4410..6251)
                     /gene="nuoL"
                     /locus_tag="ACHU9J_000080"
     CDS             complement(4410..6251)
                     /gene="nuoL"
                     /locus_tag="ACHU9J_000080"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1903747.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6248..6550)
                     /gene="nuoK"
                     /locus_tag="ACHU9J_000081"
     CDS             complement(6248..6550)
                     /gene="nuoK"
                     /locus_tag="ACHU9J_000081"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1903748.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6547..7101)
                     /gene="nuoJ"
                     /locus_tag="ACHU9J_000082"
     CDS             complement(6547..7101)
                     /gene="nuoJ"
                     /locus_tag="ACHU9J_000082"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1903749.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7113..7655)
                     /gene="nuoI"
                     /locus_tag="ACHU9J_000083"
     CDS             complement(7113..7655)
                     /gene="nuoI"
                     /locus_tag="ACHU9J_000083"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1903750.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7670..8647)
                     /gene="nuoH"
                     /locus_tag="ACHU9J_000084"
     CDS             complement(7670..8647)
                     /gene="nuoH"
                     /locus_tag="ACHU9J_000084"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1903751.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8644..11370)
                     /gene="nuoG"
                     /locus_tag="ACHU9J_000085"
     CDS             complement(8644..11370)
                     /gene="nuoG"
                     /locus_tag="ACHU9J_000085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1903752.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11423..12760)
                     /gene="nuoF"
                     /locus_tag="ACHU9J_000086"
     CDS             complement(11423..12760)
                     /gene="nuoF"
                     /locus_tag="ACHU9J_000086"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1903753.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12757..13257)
                     /gene="nuoE"
                     /locus_tag="ACHU9J_000087"
     CDS             complement(12757..13257)
                     /gene="nuoE"
                     /locus_tag="ACHU9J_000087"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1903754.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13260..15062)
                     /gene="nuoC"
                     /locus_tag="ACHU9J_000088"
     CDS             complement(13260..15062)
                     /gene="nuoC"
                     /locus_tag="ACHU9J_000088"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1903755.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15156..15818)
                     /gene="nuoB"
                     /locus_tag="ACHU9J_000089"
     CDS             complement(15156..15818)
                     /gene="nuoB"
                     /locus_tag="ACHU9J_000089"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1903756.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15834..16277)
                     /gene="nuoA"
                     /locus_tag="ACHU9J_000090"
     CDS             complement(15834..16277)
                     /gene="nuoA"
                     /locus_tag="ACHU9J_000090"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1903757.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16905..17843)
                     /gene="lrhA"
                     /locus_tag="ACHU9J_000091"
     CDS             complement(16905..17843)
                     /gene="lrhA"
                     /locus_tag="ACHU9J_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1903758.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18768..19985
                     /gene="alaA"
                     /locus_tag="ACHU9J_000092"
     CDS             18768..19985
                     /gene="alaA"
                     /locus_tag="ACHU9J_000092"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1903759.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20069..20668
                     /gene="yfbR"
                     /locus_tag="ACHU9J_000093"
     CDS             20069..20668
                     /gene="yfbR"
                     /locus_tag="ACHU9J_000093"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1903760.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20727..22559)
                     /locus_tag="ACHU9J_000094"
     CDS             complement(20727..22559)
                     /locus_tag="ACHU9J_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1903761.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22646..23294)
                     /gene="hxpA"
                     /locus_tag="ACHU9J_000095"
                     /pseudo
     CDS             complement(22646..23294)
                     /gene="hxpA"
                     /locus_tag="ACHU9J_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23305..23799)
                     /locus_tag="ACHU9J_000096"
     CDS             complement(23305..23799)
                     /locus_tag="ACHU9J_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1903762.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23882..24337)
                     /gene="yfbV"
                     /locus_tag="ACHU9J_000097"
     CDS             complement(23882..24337)
                     /gene="yfbV"
                     /locus_tag="ACHU9J_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1903763.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24675..25877
                     /gene="ackA"
                     /locus_tag="ACHU9J_000098"
     CDS             24675..25877
                     /gene="ackA"
                     /locus_tag="ACHU9J_000098"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1903764.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25952..28096
                     /gene="pta"
                     /locus_tag="ACHU9J_000099"
     CDS             25952..28096
                     /gene="pta"
                     /locus_tag="ACHU9J_000099"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1903765.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28286..29806
                     /gene="yfcC"
                     /locus_tag="ACHU9J_000100"
     CDS             28286..29806
                     /gene="yfcC"
                     /locus_tag="ACHU9J_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1903766.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29839..30381)
                     /gene="yfcD"
                     /locus_tag="ACHU9J_000101"
     CDS             complement(29839..30381)
                     /gene="yfcD"
                     /locus_tag="ACHU9J_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1903767.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30439..30990)
                     /gene="yfcE"
                     /locus_tag="ACHU9J_000102"
     CDS             complement(30439..30990)
                     /gene="yfcE"
                     /locus_tag="ACHU9J_000102"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1903768.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31046..31690)
                     /gene="yfcF"
                     /locus_tag="ACHU9J_000103"
     CDS             complement(31046..31690)
                     /gene="yfcF"
                     /locus_tag="ACHU9J_000103"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1903769.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31826..32473
                     /gene="yfcG"
                     /locus_tag="ACHU9J_000104"
     CDS             31826..32473
                     /gene="yfcG"
                     /locus_tag="ACHU9J_000104"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1903770.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32530..32892
                     /gene="folX"
                     /locus_tag="ACHU9J_000105"
     CDS             32530..32892
                     /gene="folX"
                     /locus_tag="ACHU9J_000105"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1903771.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32913..33806
                     /locus_tag="ACHU9J_000106"
     CDS             32913..33806
                     /locus_tag="ACHU9J_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1903772.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33854..34744)
                     /gene="rpnB"
                     /locus_tag="ACHU9J_000107"
     CDS             complement(33854..34744)
                     /gene="rpnB"
                     /locus_tag="ACHU9J_000107"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1903773.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34941..35714)
                     /gene="hisP"
                     /locus_tag="ACHU9J_000108"
     CDS             complement(34941..35714)
                     /gene="hisP"
                     /locus_tag="ACHU9J_000108"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1903774.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35722..36438)
                     /locus_tag="ACHU9J_000109"
     CDS             complement(35722..36438)
                     /locus_tag="ACHU9J_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1903775.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36435..37121)
                     /gene="hisQ"
                     /locus_tag="ACHU9J_000110"
     CDS             complement(36435..37121)
                     /gene="hisQ"
                     /locus_tag="ACHU9J_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1903776.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37211..37993)
                     /gene="hisJ"
                     /locus_tag="ACHU9J_000111"
     CDS             complement(37211..37993)
                     /gene="hisJ"
                     /locus_tag="ACHU9J_000111"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1903777.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38214..38996)
                     /gene="argT"
                     /locus_tag="ACHU9J_000112"
     CDS             complement(38214..38996)
                     /gene="argT"
                     /locus_tag="ACHU9J_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1903778.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39262..39831)
                     /gene="ubiX"
                     /locus_tag="ACHU9J_000113"
     CDS             complement(39262..39831)
                     /gene="ubiX"
                     /locus_tag="ACHU9J_000113"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1903779.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39926..41443)
                     /gene="purF"
                     /locus_tag="ACHU9J_000114"
     CDS             complement(39926..41443)
                     /gene="purF"
                     /locus_tag="ACHU9J_000114"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1903780.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41480..41968)
                     /gene="cvpA"
                     /locus_tag="ACHU9J_000115"
     CDS             complement(41480..41968)
                     /gene="cvpA"
                     /locus_tag="ACHU9J_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1903781.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42227..42889)
                     /gene="dedD"
                     /locus_tag="ACHU9J_000116"
     CDS             complement(42227..42889)
                     /gene="dedD"
                     /locus_tag="ACHU9J_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1903782.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42879..44147)
                     /gene="folC"
                     /locus_tag="ACHU9J_000117"
     CDS             complement(42879..44147)
                     /gene="folC"
                     /locus_tag="ACHU9J_000117"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1903783.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44217..45131)
                     /gene="accD"
                     /locus_tag="ACHU9J_000118"
     CDS             complement(44217..45131)
                     /gene="accD"
                     /locus_tag="ACHU9J_000118"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1903784.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45287..45946)
                     /locus_tag="ACHU9J_000119"
     CDS             complement(45287..45946)
                     /locus_tag="ACHU9J_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1903785.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46029..46841)
                     /gene="truA"
                     /locus_tag="ACHU9J_000120"
     CDS             complement(46029..46841)
                     /gene="truA"
                     /locus_tag="ACHU9J_000120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1903786.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46841..47854)
                     /locus_tag="ACHU9J_000121"
     CDS             complement(46841..47854)
                     /locus_tag="ACHU9J_000121"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1903787.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47920..49056)
                     /gene="pdxB"
                     /locus_tag="ACHU9J_000122"
     CDS             complement(47920..49056)
                     /gene="pdxB"
                     /locus_tag="ACHU9J_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1903788.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49155..50150
                     /gene="flk"
                     /locus_tag="ACHU9J_000123"
     CDS             49155..50150
                     /gene="flk"
                     /locus_tag="ACHU9J_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1903789.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50147..51325)
                     /locus_tag="ACHU9J_000124"
     CDS             complement(50147..51325)
                     /locus_tag="ACHU9J_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1903790.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51600..52820)
                     /gene="fabB"
                     /locus_tag="ACHU9J_000125"
     CDS             complement(51600..52820)
                     /gene="fabB"
                     /locus_tag="ACHU9J_000125"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1903791.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52979..54985
                     /gene="mnmC"
                     /locus_tag="ACHU9J_000126"
     CDS             52979..54985
                     /gene="mnmC"
                     /locus_tag="ACHU9J_000126"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1903792.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55106..55384)
                     /locus_tag="ACHU9J_000127"
     CDS             complement(55106..55384)
                     /locus_tag="ACHU9J_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1903793.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55418..55966)
                     /gene="epmC"
                     /locus_tag="ACHU9J_000128"
     CDS             complement(55418..55966)
                     /gene="epmC"
                     /locus_tag="ACHU9J_000128"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1903794.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55966..56775)
                     /locus_tag="ACHU9J_000129"
     CDS             complement(55966..56775)
                     /locus_tag="ACHU9J_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1903795.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56775..57599)
                     /gene="mepA"
                     /locus_tag="ACHU9J_000130"
     CDS             complement(56775..57599)
                     /gene="mepA"
                     /locus_tag="ACHU9J_000130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1903796.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57603..58688)
                     /gene="aroC"
                     /locus_tag="ACHU9J_000131"
     CDS             complement(57603..58688)
                     /gene="aroC"
                     /locus_tag="ACHU9J_000131"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1903797.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58723..59655)
                     /gene="prmB"
                     /locus_tag="ACHU9J_000132"
     CDS             complement(58723..59655)
                     /gene="prmB"
                     /locus_tag="ACHU9J_000132"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1903798.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59821..60372
                     /gene="smrB"
                     /locus_tag="ACHU9J_000133"
     CDS             59821..60372
                     /gene="smrB"
                     /locus_tag="ACHU9J_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1903799.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60494..61366)
                     /locus_tag="ACHU9J_000134"
     CDS             complement(60494..61366)
                     /locus_tag="ACHU9J_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1903800.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61353..61877)
                     /locus_tag="ACHU9J_000135"
     CDS             complement(61353..61877)
                     /locus_tag="ACHU9J_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1903801.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61874..62344)
                     /locus_tag="ACHU9J_000136"
     CDS             complement(61874..62344)
                     /locus_tag="ACHU9J_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1903802.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62341..62889)
                     /locus_tag="ACHU9J_000137"
     CDS             complement(62341..62889)
                     /locus_tag="ACHU9J_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1903803.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62864..63615)
                     /locus_tag="ACHU9J_000138"
                     /pseudo
     CDS             complement(62864..63615)
                     /locus_tag="ACHU9J_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63635..66277)
                     /gene="yfcU"
                     /locus_tag="ACHU9J_000139"
                     /pseudo
     CDS             complement(63635..66277)
                     /gene="yfcU"
                     /locus_tag="ACHU9J_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66359..>66916)
                     /locus_tag="ACHU9J_000140"
     CDS             complement(66359..>66916)
                     /locus_tag="ACHU9J_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1903804.1"
                     /translation="KFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIV
                     PDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSG
                     NYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKA
                     WLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16640 a        18159 c        16694 g        15423 t
ORIGIN      
        1 ttatttttac caaccctggg tcactgcatt atcgccaacg ccccgacacg tcagtcctga
       61 gcaattagaa aaaacagttc gttatcttac acaaactgtg catccacgta atgccgacaa
      121 tattgataat ctgaataggt ccgtagagta tataaaagag ttctttgtca gtagcggtgc
      181 cagagttacc gcgcagagcg tccccgattg gctattacga ctctgcgcct ggtagccagg
      241 attaccctta cccgcaatgt cctggcttta tcccgatcgg ggggacttta ttgcctggtc
      301 ggcagaatac aggatatcaa cgccgttcgt caggtaaaaa cggcattgtt atcatcccgg
      361 gatttatctg tttattctat gaatgcccca tggtttattc ccggtattga tttctctgac
      421 cacctgaatt attggcaaca cgatattccc gccgtaatga ttactgacac cgctttttat
      481 cgtaataaac aataccactt gcccggtgat acagcagaca gattgaatta tcagaaaatg
      541 gctcagatgg tgttataacc ttgttacaca acagtaaata ataaaaattt ctcgaggcgg
      601 gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga tgcgaaacat
      661 aaaattcaat ggaggaaaaa taaatgggaa tgattggtta ttttgcagaa attgactcag
      721 aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat atacatgaca
      781 cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta cactttgggc
      841 tgacaggcac ccctgctttt gatcccgcga agaatgatcc gctgagtcgt gccgtgcttg
      901 gtgaacacag cctcgaagat ggcatagatg gcttcttggg attgacctgg aatcaggagt
      961 tagccgcgac aatcgaccga ctggaatcac tggatcgaag tgagctgcgt aaacagtttt
     1021 ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca gaagagttag
     1081 aggggcaact ctttgcttcg atcatgctga tatggaaaag ctgattagcg cgtatcgacg
     1141 tatgctgcga caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac
     1201 caaaaaaaac cgccgaacat gtcggcggct ttctgactta caaactgata gcttacatca
     1261 gcggcattgc taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta
     1321 ccaacagtgc agagatcagc accacgatac cgcctgcgct gtactgccag tttgacggtg
     1381 catcgcgacc cggttgttcc ggggcgtgca ggtacaggct caccgccacg cgcaggtagt
     1441 agtaaaggcc gcttgccgaa ccgacaacca cggcgcccac cagccaccac aagtgtgcct
     1501 ggacaccgac cgccagcacg tagaacttac caataaagcc cagcgtcatc ggaataccgg
     1561 ccagagacag catcatcacc gtcatcaccg ccgcgaggat cgggcgatgc cagaacagtc
     1621 cgcggtaaga gtacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga
     1681 ccacgccgaa cgcgccgagg ctgctaaaca gataaccggc caggtaaacc cctaccgctt
     1741 ccatcgacat ctcgccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag
     1801 agatagatga gtaaccgagc agacgtttga tattggtctg gctcagcgcc atcaggttac
     1861 cgaagatgat ggaggcaaag gcgataatcg ccagcaccac gcgaatcgct tcgctgtcac
     1921 ccatcggtgc gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg
     1981 tcgccaggaa agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt
     2041 ggaacggcac cagagagagt ttgaagccga ggccaacaat catcaggccg aaacctgcca
     2101 gcaacagcgg ctcgttgagc ataccgtcgc caaggttttt acccaacgcg acaaacgaca
     2161 ggtcgccaga ctgcgcatac accagcgcca taccaaacag caggaaagaa gacgctgcgg
     2221 cagaaaggat ggtgtatttg atactggctt ccagtgaacg tttctggcgg aaagcgtaac
     2281 cgaccaggcc aaacagcggc aaagagatca gttcgatacc gaggaacaga gacgccagat
     2341 ggttggcatt cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact
     2401 cgtccttgtt gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc
     2461 tcgccaacaa taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg
     2521 taacgtccat agcgcctgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc
     2581 caataaccga gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca
     2641 caaccaccac cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag
     2701 ttattgtcat ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga
     2761 tattgccaat cgcggagtgc gaggtatcca gaatcggctg cggatagaag ccctgcagta
     2821 ccagcagcac caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt
     2881 cctggctggc aatctcgctt ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg
     2941 aataaacaga tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct
     3001 ggaagctgcc gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa
     3061 gcgttgccac cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac
     3121 cgcccatcat gcgcatgtcg cgggtatgga tacgttcata aagctgacca caaagaataa
     3181 acagacccgc cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca
     3241 actggctgcc ggtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg
     3301 cgatcagacg tttgatatcg gtctgggcga aggccatcca cgcaccgtag aagatgccga
     3361 taacacccag ccacatagca attggcgcga actctgccga cgcgttcggg aacagcggca
     3421 gggagaaacg cagcaaaccg taagcggcag ttttcagcaa gatccccgcg aggtcaacgg
     3481 aaccggcggt cggagcctgg gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg
     3541 gcattttgac tgcgaaggcg atgaagaagc ccagcattaa caggtattcc gcaccactgg
     3601 acattggcgt attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt
     3661 gaacaaaaac cagcgccagg atggcaatca acatcaccag accactcgcc tgggtgtaaa
     3721 tgaagaactt ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca
     3781 gtgcgatcag gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca
     3841 tgtcgatggc aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga
     3901 agaagccctg atatttttcg atctctttcc acgaacatag taccgccagc acaccgagca
     3961 gaccggtcag cacgaccatc agcagcgaca gcccgtcaat ggcgagatga atagagatac
     4021 ccaaacgcgg gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt
     4081 gcgtcagtga ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca
     4141 tggtgatcag cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca
     4201 ggaagccgcc aataaaggga attaatatta gccagggtag taacatggcg atctttattc
     4261 cttgtaaaag tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt
     4321 cggactgggg gtagtgtcgg atgcaccgcg aaggcggctt atccgaccta cgttcttata
     4381 tgcccagggg caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc
     4441 acgaccgcac cgatgctcat ggatgccaca taccagcgca gatagccgtt ctcacttaac
     4501 agcagacctt tacctgcaaa gcgggaaagg acagccggaa tgttcatcat tgagttcagc
     4561 ggatcgcgtt tcagcaacca ggcaataccc aggaacggcc tgacgaacac tttgtcgtac
     4621 agccagtcaa agccccaggc gttgtaccac caggtactca gcaggcggcc cggcgcactg
     4681 ttggcgatgg aggtcaccag agtacgttta cccagccaca gccaagctgc cagcagaatg
     4741 ccgacaatcg cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt
     4801 tccgtcgttt gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg
     4861 gaaaggatca gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg
     4921 tgaatttgtt cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc
     4981 ataaacgcac cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc
     5041 gcgaggatct catccttact gaagaagccc gcagtgacca gcggcagtgc cgacagtgct
     5101 gcgccgccca ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc
     5161 ttgaagatgt tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc
     5221 agcgctttaa agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg
     5281 ccaagcgcga ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg
     5341 atgtcggtct gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaatg
     5401 cccaccagat gcagaatttc cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg
     5461 tagacacccg cggttaccat ggttgcggcg tggatcagcg cggagacagg cgtcgggccc
     5521 gccatcgcgt cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg
     5581 cccagcagca tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt
     5641 gccagttcca ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt
     5701 gcgaaagcga ggaaaacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg
     5761 ccattcttcg gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct
     5821 tcccagccga ggtacatcag cagcaggttg tcggcaagca ccagaactac catgctggcg
     5881 atgaacaggt tggtgtaggc gaagaagcga gagtagccct cttcaccgcg catgtaccag
     5941 gaggcgtaca tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac
     6001 aggccgtcca gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac
     6061 agcggctggc tgtatgcctg ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt
     6121 accagcgccg ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag
     6181 cgcccacggg agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca
     6241 agcatgttca tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt
     6301 gaagttgcag cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga
     6361 gaatgtacat cacctgaccg tcggtctgac cccagtagct tccggcgaca acaaaggcca
     6421 gcgcggaggc gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga
     6481 taaccagacc ggttaagcca agaatgaata agattgccgc gaggatcagt ccatgttgta
     6541 aggggatcat gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag
     6601 cacttcaccc gcacgctctt cacgaccgac gtggaaggcc acaaccagac ctgcgagcag
     6661 cagcatagaa gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc
     6721 tttagcactg attggcgtac cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac
     6781 aatcaccacc agcatgatgg ccgacaaaat tgccggacca atccacacct gcggtttcag
     6841 ccactggcgt tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac
     6901 gaacagcacc ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc
     6961 gcccagtgag aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc
     7021 gtgtaccgga ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca
     7081 gatataaaaa gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata
     7141 ggcttggctt cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc
     7201 cggtagaagt tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttttcg
     7261 taaaccagat cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc
     7321 gtggtcggac aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg
     7381 aaaaattccg gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg
     7441 accgggcagg ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg
     7501 cgggtcagaa cgatacgacc acgataacgg ggcggcagat agatcggctc ttccgggtac
     7561 attcgcgttt cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg
     7621 gtgccgaaac ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct
     7681 gccagagaat gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt
     7741 tccagccgaa ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa
     7801 tgaacatcat cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg
     7861 ggccttgcca gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga
     7921 tgtactcacc cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat
     7981 ccgccagttc ctgctcggct tccggctggt caaacgggtg acggtgacat accgccacgc
     8041 ccgcgatggc aaaggtaata aaaccaaaga attgcgggat aacgttccac acatgcgcct
     8101 ggctgttgac gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg
     8161 aaagcccgag gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca
     8221 acgagtattt gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg
     8281 ccatcatcag gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga
     8341 ctggcacaat cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa
     8401 agatgacgcg atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt
     8461 ccgcaaccag ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga
     8521 acagacccag caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca
     8581 gcaggatcac caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc
     8641 aactcattgt tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat
     8701 accggacata cccatcggca agcccacctg ccctgccgtc agcccttcag cgatttcaac
     8761 cggcagcgtg accgtattgc catcgtaact aaaggagacg tgtgtacctg cgttcacacc
     8821 taacttcgcg gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa
     8881 gaccggagca cgctgtgaca attcatcgct gccaaacagg tggtaatacg gcgcgatacg
     8941 ccatttcccg tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt
     9001 ttcgctggtt tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc
     9061 gtcctggaat ttgttccacg cctgcgggga gttccagccc ggcgcccagg caaacggcac
     9121 ttgcgaacgg tgcgcagtcg gctggttgtt accttccatc gagaaggtga acatggtgtc
     9181 aatatcctgc ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc
     9241 gctgtaacgg tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg
     9301 cgcagcatct ttgatacctg ccagttccgg gattttcgcc acaacagcgt caatcacatg
     9361 gtcgagctgc gtccagtcca cttcacggct cagcagggtg ctgtgcagcg agtgtaacca
     9421 gcgccagctt tccagcatga cagttttgct gtcgtaataa gcaggatcgt aaacctggaa
     9481 gaaacgttgg gcgcggcctt cgttgttgat caccgtaccg tcgctttcag caaaactggc
     9541 agcagaaagt accagatggg cgttttccat aatcgctgtg cgttgatgat caaccaccat
     9601 caccagcggc gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag
     9661 atcgttttcc agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg tcaacgcttc
     9721 ttcaagcgaa ccgccaccca taatgcccag ccccatgctg ttgacggaac gggcaatcat
     9781 ggtgatacca acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg
     9841 aatcacctct atgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc
     9901 cagtgcctgc acgatgacgt cgattttgct ttgcagctca ggttcgatac cgtcaaccgc
     9961 tggcgcagag ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac
    10021 cggtgcgcgg taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac
    10081 aaacagcgga tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc
    10141 cactttctgt gctgccgcca tttcgcgcgc tttacctttc acagcctgac gcactgccag
    10201 cgcgacgcgc gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga
    10261 ttcgatttcg cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag
    10321 ttgcagacgt tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag
    10381 ttcacgcagc gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt
    10441 cttcgactga cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat
    10501 gaaatcatcg ccacgacgct gtactggctg acgcggacga tccttcaggt tgacgtaacc
    10561 gtaaccgaaa cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt
    10621 ttcgatacga cgcagttcgc cgtaacgttc accggggctg atgttacagc cgatggaaca
    10681 ttgctggcag atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg
    10741 cgttttgtcg gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct
    10801 ttccagcgtg ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc
    10861 cagatctgta ccgtcagcgt aatctttgta gtaacgcaca cagcggtagc aggcgatgca
    10921 gcggttcatt tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt
    10981 gaaacggtag cgacggaagc tgtgtccggt catcacagtc atatcctgaa gatggcagtt
    11041 accgccttct tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac
    11101 actttcacgg aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc
    11161 cggtgtcata caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt
    11221 caccgcacac tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg
    11281 aatatcaagg cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta
    11341 ttctttgccg tctacatgaa ttgtagccat tagcatgctt ccagttttct cggtcaggga
    11401 ccgagcgtta atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat
    11461 taatcaaatg ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat
    11521 atttgatggc gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac
    11581 ccgggcctaa gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tggccttcgc
    11641 cacgctccag cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac
    11701 accagccgca ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt
    11761 tgatctcatg gtcaaccgcc atcgccagcg ccgtacccag acggctgccc gctttaccga
    11821 tactttcgaa ttccatcggc agatcaaggt gcgcttcggt caggaagtca gtccccgcgc
    11881 cgcctggctg ccaggcttta aatttcagac catcacgcat accaccggcg taatcttcga
    11941 ggatctcgcg tgcggtggtg ccgaacggca gttcccacag tcccggattt ttcacccgac
    12001 cggagaagcc catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact
    12061 ccacgccgtt agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg
    12121 tcggtttgcc ccatacgccg gaggttgccg ggaagggggg tttcgagcgt gggttagcac
    12181 gacgcccttc cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc
    12241 cggtatggac gaacagttcg aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc
    12301 ccgcttcagt ggcttcggca atggcgcggc gcagatttac tgccgcttcg atatattcgc
    12361 cacgcaggaa gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt
    12421 ccaccagcag gtgcggcagt tgctccatca acaggcggtc tttataggtg cccggctcca
    12481 tttcatcggc gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc
    12541 tccacttcag gccagtcgaa aagcccgctc cgccgcgccc tttcagacca gcgtctttta
    12601 cctgattaac gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac
    12661 cgtttttgct gcggtattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg
    12721 tcagcggatg cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc
    12781 agggatcgct tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc
    12841 tttatcacag ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt
    12901 ttgccctggt ttgatgttca gctttttctc gagcgccgcc tgaataccct gatacccgtt
    12961 gatatgacag accacgctgt cgcaataacg gatcacatgg cgaccaaccg gctggcggaa
    13021 gatctgactg tagaacgttg ccacaccttc gacgtcgctt gccggaatac ccagcacatc
    13081 ggcgatcgcg tggatcgcac catccggcac ccagccacgc tgcttctgaa cgattttcag
    13141 cgcttcaatg gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc
    13201 acgctctgcc gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat
    13261 tagcggtcca catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac
    13321 accaggctgc cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg
    13381 atacgagtgc gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc
    13441 ttggtcgcct caatcatctg gaaagattca ttggcaggca tcaccggccc ccacgacact
    13501 tgcaggaagt gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc
    13561 gtggtcagcg ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca
    13621 agaatgcgca gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca
    13681 gaaacaccac caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc
    13741 gccttacgca cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac
    13801 tccagcgcct ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta
    13861 ttttgcagcg ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca
    13921 cgcagcaggc gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac
    13981 gccgggtgca tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg
    14041 gtaaaggcga agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac
    14101 agcaggtggc tgttgatacg gaacagttcg gagagcataa cgcgaatgac gttaacgcga
    14161 tccggcacgg tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg
    14221 cagccgccga ggtattcaat acggtcggta tacggaatgt agctgtgcca ggactggcgc
    14281 tcgcccattt tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc
    14341 tcttcgccgt cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg gtggttcgga
    14401 ccgaggttga ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc
    14461 ggtttgaagg tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc
    14521 gagaattcgg tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc
    14581 ggcatcatga tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag
    14641 gtttcacgct cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg
    14701 tcgttttctg ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga
    14761 tggtagaaaa cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg
    14821 tccatgccgt gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg
    14881 ccaacttcca gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc
    14941 tgaacagtaa aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca
    15001 agatgatcac gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc
    15061 attgcaaatg gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat
    15121 aagcgaagaa aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg
    15181 ttggttacgg caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga
    15241 taaacgccct gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc
    15301 tgcaacagca tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac
    15361 acatcaaccg ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacatacca
    15421 ccagagttgg cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac
    15481 agacgctgaa taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca
    15541 gcctgacgcg gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg
    15601 gtaaacgaag tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt
    15661 gagtttttac gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta
    15721 acttcttgct ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc
    15781 tcaccgttgg gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt
    15841 tgacgattag cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc
    15901 gtccagtcca gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata
    15961 aaaattgcag cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg
    16021 aacagataca gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg
    16081 gcggacaggc gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt
    16141 ttcgacctcg cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac
    16201 aggccaatgg caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg
    16261 gatgttgaca tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg
    16321 gcagatggac gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag
    16381 aaattctttt atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa
    16441 ctttaacaaa agtttcttca cattagttta cataatatca acaccattag catttaatgc
    16501 cctttcacgc cagatccttg acgactccag gataattaga tgttgttgat cgtgtccgtt
    16561 gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa tttgtgtcga ttcctccccc
    16621 caaaagagag tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag
    16681 caacaaattt acgtattttt aacatcattg tagcaggtga tttttttcag gcgattattt
    16741 gtgcgttcgg gacgtgaatc tctggtgatt gaaaaatgaa cagttttgta cgttctgcac
    16801 tatgcgagga aggattttta ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc
    16861 tcgtttttac actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt
    16921 tcaatcaaca atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta
    16981 tggtagctct ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca
    17041 tagcacagca aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca
    17101 cgcaggtccg ggctcatcat ttcaatcggc cttgccgtca cgccaagacc ggctttcact
    17161 gccgcacgaa cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct
    17221 ttattaagcg tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga
    17281 gggatcggct cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag
    17341 gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact
    17401 tcctgggatt ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct
    17461 aatttcggat aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat
    17521 tcatctgaag caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag
    17581 gcctcatcat taaaacgcag tattttcctg gcgtaaccaa gaagttgaat gccatgttca
    17641 gttaacagtt tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga
    17701 cgctgcattt gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca
    17761 aaagtgttca gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt
    17821 atcggacgat ttgcacttat catatcatat attatcactt actggcggct catactgagc
    17881 tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct ccctggcagt
    17941 ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa aagcgactca
    18001 cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg atcgcatatc
    18061 tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa atgtaaatgt
    18121 gagtccttgt tccactctcg tgcagcatcg ctggtcatac gcgaacacgt acaaacagca
    18181 atggtgaggc ggcatcaaga gcagggatcc gtcatttatg cgagcatttt accccaaaaa
    18241 ccttttattt ataagggtca ttgcgaatta tctgatgcaa agttatgtta tgttaggcaa
    18301 agtaatcttc tattattaat aagcacatca aaaccttttt gaatattaaa taataattaa
    18361 ttagcatcat cctcattcat taattccgct taacaatagt ttcacacctt gcccccattg
    18421 cgacacctcg gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc
    18481 gtttcattag tgaattctta aatgaggcat tttcacacaa ttatcttaca gataaaaaaa
    18541 ccagacttgc aattaagaat cagaacaatt cactatataa cattgcatgc aaagcatata
    18601 cacctcatta ttttgtcatt attaagttat taacagcaca atcgagcctt cccctctggc
    18661 aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg
    18721 acttccacgg cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg
    18781 aaaaatccag caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag
    18841 caaaacgcct ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc
    18901 cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg
    18961 ctcaagggta ttgcgattcc aaagggcttt actccgcgcg taaagccatc atgcagcact
    19021 accaggctcg tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat
    19081 cggagcttat cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc
    19141 ctgcaccaga ttacccactc tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc
    19201 attatctttg cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa
    19261 ttacgcctcg tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat
    19321 attccaaaga gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct
    19381 tcgccgatga aatttatgac aaaattctct acgacgatgc tgagcatcac tcaattgcgc
    19441 cactggcacc tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg
    19501 caggcttccg tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca
    19561 tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg
    19621 ccattcagac cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc
    19681 gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct
    19741 gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca
    19801 ttcacgacga tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc
    19861 aagggacggc attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg
    19921 tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc
    19981 tgtaatctta atttcactgc cggagattgc atccggcagc gttatcccgc cacaatgacc
    20041 tgatgatgtc atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc
    20101 ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac gtgcggacgg aaaatgtgtc
    20161 cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcgaaa
    20221 atttggcggt aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc
    20281 agaagtgctc accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca
    20341 ggagtacaag gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga
    20401 gctgcgggat atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc
    20461 gctggtgaaa caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc
    20521 cggaaataat gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg
    20581 cagccaggag atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga
    20641 tgagattagc caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa
    20701 gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca
    20761 caaaccgcca tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg
    20821 taatttcccg gacctaaaac cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg
    20881 gcggatgctg ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg
    20941 gcagcagcca gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc
    21001 ccaataaccg ccgacaagac aaacaaacac cccagcatca tatgtggccc gtaaccaccg
    21061 ccaatgtcca tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca
    21121 gcaaacggca tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg
    21181 ctttcagcat ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca
    21241 ggattaggaa tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca
    21301 tggggtgcct ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct
    21361 acgaagtcgc ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg
    21421 atgatatcgc ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg
    21481 cgctttagcc ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat
    21541 ttaccaatca gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc
    21601 tggtcagaaa aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat
    21661 tgccggagat cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat
    21721 tcagaaaccc cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg
    21781 atgacgttag cgccataacg ctcacgtaat ttgagatcat ccagccgttg accaatcatt
    21841 ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata
    21901 agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc
    21961 agcatgaaac gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata
    22021 ggtgttacgc taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact
    22081 accaggttcg gcggcgtcgc caccagcgtc atcatgccgc ttatcagccc ggcaaaactc
    22141 aacggcatca tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg
    22201 ggaataaaga tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc
    22261 agcatcaaca aaaccaacat tttgatttca ctgttgcccg caactttgac cagccatgtt
    22321 cccattacgg tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca
    22381 atcaggacaa cgttaggatc agaaaagccg gaaaatactt cggggactgt cagcgttccg
    22441 cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc
    22501 gcaaacaaga caatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca
    22561 acttatcctt gttaattgag ggagatgtct tgattctgcc ataaaaaagc cccgacgaga
    22621 cggggctaaa tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg
    22681 tcacagtaat ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat
    22741 ctgccggagc gttaaccgca atgacatgac aacccgccgc caggccagaa agcacgccag
    22801 cggagcatct tccaccacca cacactcctg cggcgcaagc cccagcagct gcgcgcctaa
    22861 cagatacgca tcaggttctg gttttccgcg cttcactcgc tcagcggtta caaacacctc
    22921 tggtgcggga agcccagcta ttttatggcg cgctcgcgct accggcatgg agccagaagt
    22981 cacaatggcc cacggaatac ctgctttatt caaatgactg agtaaggcga ttgcccccgg
    23041 aagcgggtaa taccttcggt ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg
    23101 gcaatatcag cctcggattt gcccgccata aaatggcgca gagaggtgat cgcctgttta
    23161 ccgtgaatga aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc
    23221 cacgcccgtt ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa
    23281 cctttgcacc gcacgcgggc ctcctcaggc attgataatt tgattaattt cgttggcgct
    23341 caaatggtac tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca
    23401 cattggcgtc tgggcgttaa aaccgtgagt tccggcgtca aaatgggtat agcgcccttc
    23461 cacattaacc ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc
    23521 gaggaaggtg acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac
    23581 atgcaacgca tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag
    23641 ctcgccaaac tcgcgatcca attcacgcat ctgtaatccg taacctcgct caataattgt
    23701 ttgcaggcga cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg
    23761 attagacaaa ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct
    23821 actacggtga aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca
    23881 attacaaatc atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag
    23941 ctaatgcctg gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca
    24001 atttaccgcg aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac
    24061 gcttgcccag ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa
    24121 ctgccggccc aagctgaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg
    24181 gcggcataaa acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg
    24241 gggcaaggcg tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc
    24301 taaaaaaatt aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata
    24361 aagattcaaa aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc
    24421 ttccataccc actatcaggt atcctttagc agcctgaagg cctaagtagt acatattcat
    24481 tgagtcgtca aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga
    24541 ctcaagatat ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat
    24601 gtcggtgtca tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa
    24661 ttataggtac ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac
    24721 tgaaatttgc catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat
    24781 gtttccacct gcctgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg
    24841 ctttaggtgc aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg
    24901 cacaaaaacc agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg
    24961 gcgaaaagta taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg
    25021 cagcttcttt tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga
    25081 aatctttccc acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga
    25141 ctatgccgga agagtcttac ctctacgccc tgccttacaa cctgtacaaa gagcacggca
    25201 tccgtcgtta cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa
    25261 tgctgaacaa accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt
    25321 ccgtttctgc tatccgcaac ggtaaatgcg ttgacacctc tatgggcctg accccgctgg
    25381 aaggtctggt catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc
    25441 acgacaccct gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc
    25501 tgctgggtct gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga
    25561 aagaagacgc gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg
    25621 cctacactgc gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg
    25681 aaaatgccgc gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag
    25741 ttgatcatga acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag
    25801 gtacccgtcc tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga
    25861 gccgcctgac tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg
    25921 ccaaatcggc ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac
    25981 cggaaccagc gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa
    26041 aggcgttcgt ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc
    26101 cgatcagact acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct
    26161 gaaaatgagc tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga
    26221 gatcgtcgca aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct
    26281 ggtcccgaca cgtaagcacc agtttgccca gtcgctgaac tacgaaatcg ctaaaacgct
    26341 gaatgcggaa atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga
    26401 gcgtatcgaa ctgacccgca acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt
    26461 tatcgttaac aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc
    26521 cgagattttc gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca
    26581 agaatccagc cctctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac
    26641 tcgtgcgatc gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa
    26701 tactcgccgc gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca
    26761 cttccgtgcc ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc
    26821 ttgcctggca gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga
    26881 aatggacgcg cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt
    26941 tatggtgaac accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt
    27001 tccggttgac gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa
    27061 cgctgactgg atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc
    27121 tgcgttccgt tatcagctga ctgaacttgc gcgcaaagcg ggcaaacgta tcgtactgcc
    27181 ggaaggtgac gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc
    27241 aacttgcgta ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt
    27301 agaactgggt gcagggattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg
    27361 tcgtctggtc gaactgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct
    27421 ggaagacaac gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt
    27481 ttccggtgct gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac
    27541 tgcaccgggc agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta
    27601 cgtttacggt gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc
    27661 gattcagtcc gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc
    27721 ctactccacc ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg
    27781 tctggcgcag gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc
    27841 ggtaatggct gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac
    27901 cgtgtttatc ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc
    27961 tgccgacctg atctccatcg ggccgatgct gcagggtatg cgcaagccgg ttaacgacct
    28021 gtcccgtggc gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc
    28081 tgcacagcag cagtaacctc gtcatcatcc gcagctttgc gctgcggata tctgaaccgg
    28141 aaataatcac tatttccggt tttttattct cttaatctgc attaatcctt tctgattatc
    28201 ttgcttaact gcgctgcatc aatgaattgc gccatttcac tttacatact taccactttg
    28261 ttttgtgcaa gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag
    28321 atgggcaatg cccgatacgt tggtgattat cttttttgtt gctattttaa ccagccttgc
    28381 cacctgggta gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg
    28441 tcaaacaaaa acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc
    28501 aggcgaacct gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct
    28561 gatgaacttc ccgtttgaag gattaacctc aggatcgaaa tacgggacag ctgttggcat
    28621 catcatgttt atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat
    28681 tgataacggt atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc
    28741 tgcgctgttt attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt
    28801 cgcctttgcc attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac
    28861 cgtcctggtg acctatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt
    28921 ttgtgtggtc gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg
    28981 catcgtggtg tgggttatcg ccactatgat tggcctgatc tttaccatgg tgtacgcctc
    29041 acgagtgaaa aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga
    29101 aaagcaggcg gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt
    29161 cctgaccgcc gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc
    29221 agaaattgcc agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt
    29281 ccgccttaac ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat
    29341 gatgatcgcc cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggtcggtaa
    29401 tggtgaagcg ggtgatgcca gcgtgttaaa taccatcctc aacagcattg ccaatgccat
    29461 tagcggtctg gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg cggtatttaa
    29521 tttcttcgtg acgtccggtt ctggtcaggc ggcgttaacc atgccgttac tggcaccgct
    29581 tggcgatctg gtcggtgtta accgtcaggt taccgtgctg gcattccagt ttggtgatgg
    29641 cttcagtcac atcatttacc caacctcagc ttcgttaatg gcaactctcg gtgtgtgcag
    29701 ggtggacttc cgtaactggc tgaaggtggg cgcgacccta cttggactgc tgtttattat
    29761 gtccagcgtc gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc
    29821 gcaattgcgg ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt
    29881 gcgcttcatc cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc
    29941 ggtgatttct tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa
    30001 gggaccgtga gagacgcagc tgaacaacgc gccccagaca cggcaatttt tatcttcgaa
    30061 atagaactgc ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc
    30121 gcgacgcgcg gattccagca gttgctcatc ggcctgaacc acaccgcctg cggtcgcatc
    30181 taacatgccg ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat
    30241 gccatcatgc acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc
    30301 ccggctggct tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact
    30361 tgccaaacga cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca
    30421 ttttgaattg tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt
    30481 gagtgcgatt acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc
    30541 tttcggaata ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac
    30601 tggcagatgg gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc
    30661 cgggccaaaa agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg
    30721 ccacggcgcg gttatcggga aatgcagcag catttgatcc acttcgctgt cgcagttgcc
    30781 gcgcacagcg ataaccttat gtgccacttc attaagccgt tcagcgactt tggctggcgc
    30841 gtaaccctcc ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag
    30901 ccactgggca ccgctttggg caaacaactc cagaacacgt tccgtcgccg gtaacgaccc
    30961 atgaatgtcc gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct
    31021 atgatactgg attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac
    31081 gctggacaga cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct
    31141 catcgccatg tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc
    31201 attcaccaaa taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt
    31261 tctctgcact ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa
    31321 catccgtcgg acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatcc
    31381 gtcgtgcacg cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg
    31441 caaatcgatc ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga
    31501 tttgtaataa cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc ttcaaatgtt
    31561 caccgctgtc gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca
    31621 cccaggcgga taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg
    31681 gtttactcat tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt
    31741 gtcactgtca cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc
    31801 agatgattaa acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg
    31861 tcacaaaatt acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga
    31921 cctggggaaa ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat
    31981 tccggcaatt gttgatcatt ctcctgccga tggcggcgaa ccgctaagcc tctttgagtc
    32041 tggtgccatt ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg
    32101 tgagcgcgcc gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct
    32161 tgggcaaaat catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg
    32221 ttatcaggtt gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc
    32281 ctggctggga ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc
    32341 ctggactcgc cagcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg
    32401 gatccgttcg cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg
    32461 ttcggatagt taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga
    32521 gaacctgcaa tggcacaacc tgccgctatt attcgtataa agaaccttcg tttacgtacg
    32581 tttatcggaa ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg
    32641 atccactacc ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat
    32701 cgcaccgtaa cgaaaaacat tattcagcat gtagagaata accgtttctc tttgttggaa
    32761 aaattaactc aggatgtgct cgatatcgca cgtgaacatc actgggtgac gtatgctgaa
    32821 gtggagatcg ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc
    32881 tggcagcgtt aaccgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg
    32941 gattaattgg tcgccatttg attccgcgtt tgctggagct gggccatcaa atcacggtag
    33001 tgacgcgtaa cccacagaaa gccagttccg ttctcggccc tcgggtgacg ctatggcaag
    33061 ggctagccga tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac
    33121 cgattgctga taaacgctgg actcacgagc aaaaagagcg tctctgccaa agccgctgga
    33181 atatcacgca aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca
    33241 tttccggctc ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg
    33301 aaccgccgca taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc
    33361 gggcgcaaag tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg
    33421 acggcggtat tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg
    33481 gttccggtcg gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct
    33541 ggctgctgga taacgacctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca
    33601 atgaacaatt tgcccatgcg ctcggtcaag cgctgcatcg cccggccatt ttgcgcgtcc
    33661 ccgcaaccgc cattcggctg ttaatgggcg aatcttcaat actggtatta ggtggacaac
    33721 gcgcgctgcc taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag
    33781 aggcgctggc ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc
    33841 cggtgttaca ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca
    33901 tcataactaa ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt
    33961 cttcgtgttt gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt
    34021 tctccttttc ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg
    34081 catcccctgt ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc
    34141 ctgtaactag cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat
    34201 gtttctgaat taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca
    34261 ccacggtaat atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct
    34321 cggcaaattc atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa
    34381 acagcatcgg gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg
    34441 ccgcaatgga ataacgcatc atgcgaaaag ccattaattc ttccagctta ctttggtgct
    34501 ctatcactac ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg
    34561 aataatattg ccgcaggtct tcatcaataa aactgtttga ttctagttta agcgtcgtta
    34621 aatcacacag tttgcgcagc ggcgcgggaa gatgaatatc aataaaatcc cgcgcggtgt
    34681 ctggatggcg taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg
    34741 tcatggcgtt ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc
    34801 caatgcagca ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg
    34861 caacttttac atttcagcgg aagctgactc gcaaattccc catgaaaata ctccggcagc
    34921 gttagccacc ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg
    34981 ctttgcgggt tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg
    35041 aggaaaatga cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc
    35101 atggttttcc cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca
    35161 ggatcgagcg ccgaggtagg ttcatcaaac agtaaaactt ccggttccat cgccagcgcc
    35221 cgcgcgatag aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc
    35281 gcacgttcgt ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc
    35341 ttgctcaggc ccaacacctg aatcggcgct tccatgacgt tttccagcac cgtcatatgg
    35401 ctccagagat tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga
    35461 tttttatcgg cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca
    35521 ttgaccacga tcgacccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta
    35581 cttttccccg atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt
    35641 gagacccctt tcagcacttc atgttcgccg tagcgtttgt gcaaatcgat aacgtttaat
    35701 ttattctcgg acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg
    35761 tttttccgct ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc
    35821 ggcaatgccg aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt
    35881 cagcagatcc ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac
    35941 ttcgttgctg tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcaacgata
    36001 cattttaaaa gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga
    36061 acggattgcc ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag
    36121 cacggtacag ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat
    36181 ctcaagcgtg tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg
    36241 aaaaatatag gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc
    36301 aatcgccaga aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag
    36361 agtgatcgcc acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc
    36421 atgtaagatt tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg
    36481 aacagcagca caccattgga aacagtggtg aaaaccaggt aaatcacgcc acagacgatg
    36541 gcgaaataga acggttccca ggtacttttg cctgccagtt gggtggcttt gaccacatct
    36601 tccaggccga gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca
    36661 atgcctggca gggcgtaacg catcatcgcc ggaaacatga tccgccgaaa cacttgccca
    36721 cgagtaaaac cgaacgccgt cgccgcctct atatgtcctt tcggcactgc cataaaagca
    36781 ccacggaacg tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg
    36841 accatcggat cgatatcaat ctgcccgacg cccatcgcct ccgtcaccgt gtttagcgca
    36901 atctgcaaac cgtagaaaat cagcagcatc aacactaagt ccggcacgcc acgaatcagc
    36961 gtggtgtacc cttcgaaaat cagccccgaa agccgatttt gcgagagctt accgccagcg
    37021 ccaattaaac cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg
    37081 agcgcaccct gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt
    37141 gtggtgaatt accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca
    37201 ccgatggcga ttagccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt
    37261 aagtaccgtc agcgcgcatt tcggcaaagg ctttgttcag tgcttcgcgc agttcgttat
    37321 cttctttacg caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg
    37381 ggccaccgaa tttgtaatct ttaccgacag gttgtttgag gaaaccttcg ctggcagcga
    37441 cctcatcctg gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct
    37501 gcccctgata cgagacgatt tcaatgcctt ttggtgccca atgttcatta ccgaacgtct
    37561 cctgggtggt gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct
    37621 gaatgtcaga atttttcgcc accaccaaac gagaatcggc agcgtacagt ttgtcggtga
    37681 aggctatttc ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct
    37741 tcttcgcttt taaggacggg attaacgcat ccagcggatt ttcgacaaac gtacattgcg
    37801 tattgatgcg tttgcataat tcctttgcca gatcgatatc gaagccaacc agttcgcctt
    37861 gtgaattttt tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg
    37921 gaatcgcagc aaatgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca
    37981 ccagtttttt cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg
    38041 ttacacttat cgtgccataa aattgaccat caaggcaaaa tattccgccc cgttagacgt
    38101 attatttttc attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa
    38161 cctggcgtgc accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca
    38221 ccgtagacat taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc
    38281 agctcgccaa gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct
    38341 acaccggtgc catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa
    38401 gctttaccag caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca
    38461 gcatccagac gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc
    38521 acatccacgc ctttgctacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc
    38581 agcacaccaa catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg
    38641 gcagcaatca aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga
    38701 cgtttatcgg taatggaaag cgacgaaata atggcgtcga tttttttcgc tttcagtgag
    38761 gggatcagcg cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac
    38821 atctcgttac cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag
    38881 aacggcgcat aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg
    38941 gaagccgctg cggagagacc gactaacaaa gacagagcga gaatcgactt cttcatacat
    39001 aaccctcaag tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct
    39061 ttcatgcagg tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa
    39121 taagacgttg cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc
    39181 gtttcgattg aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc
    39241 tgaaacaggg caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag
    39301 ggttattgca aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga
    39361 ttgcgggcga tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc
    39421 ctgagtcatt aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa
    39481 cgggcgacgc tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct
    39541 atggacaatg ccggaaaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg
    39601 gaaagaaccg gaagagatgc tggcggaaat atcgcgcgca tcgtgcgtga catcagctaa
    39661 tgcctgcact tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg
    39721 gctcatcacc agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac
    39781 gccataaatc gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg
    39841 ccctgtcaga cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc
    39901 cagaccggca ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttccactt
    39961 cgttctgacg ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt
    40021 agccctgatc aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct
    40081 ggatatccgg attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca
    40141 acccgtcagc accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt
    40201 ccgtggcgct cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag
    40261 aagcgaggta cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag
    40321 tggtgccacg gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc
    40381 ggttggcatt cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa
    40441 aggtgcggcc aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac
    40501 gagcaatttc cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat
    40561 ccagatcttc ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg
    40621 cgctgtaaac ggaaattttg tcgatgaacg agtccgggcg agcaaagtat acatactcaa
    40681 acaggcacgg attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt
    40741 cagtgatgta aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat
    40801 cgagcgctac gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt
    40861 ttcccagtac cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga
    40921 taatcatcgc cacacaggca tatgcgccgc ggattaagcg gtttgtggca gcaatggcag
    40981 cgaaaatatt gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctagcgaaga
    41041 tattaagcag aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca
    41101 gttttttacg caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat
    41161 acggggagtt aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt
    41221 aacgcacatg accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata
    41281 catcgctcac cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga
    41341 tgatgccggc ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact
    41401 ggttaaccgg cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt
    41461 aagccacatc tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat
    41521 caaaaaacca tctgatgata aaactgaact gcgggatcag ctgtgatttg ctccagtctt
    41581 cgcttttcga caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca
    41641 caccgcgcaa cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt
    41701 tctccaccaa ctggcctatc acgaagttca cgatagcacc aacaatcagg gtagcgataa
    41761 acagtaccgc gatggcaatt ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga
    41821 ctgacaggta agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg
    41881 ataacgcttc acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg
    41941 cgattatggc gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct
    42001 gttcggggcg cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccacgca
    42061 tcaataaaaa tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca
    42121 tcgtgcaatg cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg
    42181 cccaggcctg ataagacgcg ccagcgtcgc atcaggcaag actgtattaa ttcggcgtat
    42241 agcccattac cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca
    42301 gcttatcttt cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg
    42361 tggatggcga cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca
    42421 ctttatcggc atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg
    42481 ctttttcttc cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaaccttcg
    42541 gtttaggcgg ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa
    42601 actcggtgtt attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg
    42661 cccgcacctc ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg
    42721 gcatcatatc aggctcatcg cgatcgcccg cttttggaac cagcgggata gccgcaaact
    42781 catcctgata atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg
    42841 ccaccagcac gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc
    42901 gcgtcaatca cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg
    42961 tcttccgctt tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat
    43021 ttgccgttac ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt
    43081 ggcgcacaat accagtcatc aaccacgctt ttcaaccagg ccaaagttcc ggcaatatct
    43141 ttatcatgta gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata
    43201 cgcccggtga gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc
    43261 gactcgctca caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg
    43321 gcattttcac tgacttccag cccgctggca cgcagtgccg ccagcgctgt tgcggcattc
    43381 ggttgcggga caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc
    43441 cagtcatgat cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct
    43501 ttttcctgcg ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt
    43561 tttgcgctgc gaaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggtcccagc
    43621 cagtcggtat gatccagcgc aatgctggtt actacagcca catcggcatc gacaatattg
    43681 gttgcgtcca gacgaccgcc cagccctact tccagaatca ctacgtcaag ttgtgcctgt
    43741 ttgaacagcc acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg
    43801 cgtgccgatt caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcctgcccc
    43861 tgcacacgta cgcgctcggt ataacgcacc agatgcggtg aactgtagac gcccactttg
    43921 taccctgccg ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta
    43981 cccgcaacgg taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg
    44041 ctcacgcgct caaggccgag atcgatagtt ttactgtgca ggttttccag ataagaaagc
    44101 cacgaagcca gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat
    44161 tcgttacggt gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag
    44221 gcctcaggtt cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga
    44281 ttcggcgctg gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc
    44341 ggacgatgga cgatcatgtc gatggcgcct ttctcgatca ggaattcact gcgctggaat
    44401 cctggcggca gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccaatt
    44461 aacgctttcg gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg
    44521 cccatcgtcg ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc
    44581 agtgccgcag aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg
    44641 ccaccagagg cggagaagca gatcagcggg cagttatctt ccagcgcctg ctcaacggca
    44701 cgcacgaaac gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat
    44761 gccgcagcga caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt
    44821 tcgccggttt ctttctgcgc agaagccaga cggtctttat acttcttgga gtcacgaaac
    44881 ttcagcacat ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac
    44941 aggctatgca ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc
    45001 tcaagattac gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc
    45061 cacacccctt caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca
    45121 atccagctca ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg
    45181 gacgcataat gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca
    45241 gcccgaaact ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc
    45301 gtgccgcagc gcgtttgtga cgaataaatt cgaaaacccc aggcaatatt gaaacaaaaa
    45361 taatgccaac aatcatcagc ttaaggttgc tctgaatgaa ggggagcgtg ccgaagaaat
    45421 aacccgcgta ggtaaaaatc agtacccaca acagtgcgcc gatcacgtta taagcggcga
    45481 aatgacggta cgacatgtgg cccattcccg caacaaacgg cgcgaacgtt ctgacgatcg
    45541 gtacaaaacg ggcgagaata atcgttttgc cgccatgtct ctcgtaaaac tgatgagtct
    45601 tatcaagata actgcgacga aagattttcg aatccgggtt actgaacagt ttgtcaccaa
    45661 ataaccgtcc aatggtatag tttactgcat caccgacaat agcggcaatc aacataagca
    45721 ccaccatcat atggacattg agatcgttag tttccagcga cgccaatgca ccagcaacaa
    45781 acagcagcga atcaccaggc aggaatggcg ttaccaccag ccccgtttcg cagaacagaa
    45841 tcaaaaacag aatggcataa acccagacgc cgtactccgc gaccagctct gccagatgca
    45901 cgtcaatgtg caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct
    45961 tgtactcgtc atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat
    46021 tattctcgtt agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg
    46081 tcagggtaat ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc
    46141 gttctgtcct ttgccgccag caactctgct atccatctct ccggctggtt gtgggcaccg
    46201 acttccatca ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg
    46261 atatctacca ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac
    46321 ggggttcggg actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag
    46381 cattgcgcag cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct
    46441 ttactcaata ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc
    46501 gtggcgctaa atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg
    46561 tcaccaggta aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt
    46621 gtttcgaaat gcaccacctg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag
    46681 aagacggtga tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca
    46741 ctgcggactt cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc
    46801 agcgcaattt tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca
    46861 ccagtttctc ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca
    46921 ccgaccagaa ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa
    46981 gatgcggcgt acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca
    47041 caatatcttc gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg
    47101 cttcaaagtt gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg
    47161 aaatcatcag cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt
    47221 cacgcacgct accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac
    47281 cgaagaaatc ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta
    47341 acgcatcgac cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac
    47401 gcgacaaacc gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac
    47461 tgtctggtac ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt
    47521 ccggcaccac cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca
    47581 ggcaacctga gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa
    47641 aaaatgccag ctgcgcctgc gtccagtcga attcagcggc atcctgcaca gtgattgttt
    47701 taccaccaaa gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc
    47761 caaccgggaa ctgacgttca gccagcgttt caagcagggc ttcgcccaca gcgccagttg
    47821 cgcccaggac ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag
    47881 ctccctgcca atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac
    47941 ggcgttaaaa cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata
    48001 cagagatgac cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg
    48061 tatcccggcg actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac
    48121 cagccttttc agcgtcggtt gatcgagcgg gccatgcagc gtaatgcgac caaactctgg
    48181 cgcgggcagt aatgtatcca gcgcaacgtg ctgttcatgc ccaataaact tgctataggc
    48241 ttcaaacact tgcgtagtac cgcgtgcttt accttccagg gtataacctg cgatatgcgg
    48301 cgtgccgata tccacttttg tcagcaactc cacgttaagt tccggttcgc cttcccagac
    48361 atccagcact acgcttaact tctggccttc attcaggcag gtcagcaacg cagtattatc
    48421 gacgactgcg ccacggcagg cgttaatcag aatcgctccc ggcttcaggc tacggatcag
    48481 tttttcatcc gccagatgca gcgttttgta cggcccatct ttgaagagtg gcgtatggaa
    48541 agtcagaata tccgcatgct gaactaattc atccagcgag cggaaatcac cctcatcccc
    48601 acggtcagcg cgaggcggat cgcaaagcaa tgttttaatc cctaacgctt ccagtcgcgc
    48661 ctgcaatcga cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga
    48721 aaatccatcg cgttcggcaa gcatcagtag tgaggagaaa acatattcca ccaccgcaat
    48781 cgcattacag ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc
    48841 gacatggtcg gtccccgcag tggcagtgcc aacaaattta atgggttttc ctgccagcaa
    48901 agattcattc actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc
    48961 gacggggatt ggacgcccgg gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg
    49021 ggcataaggc atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga
    49081 gagttaaccg gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg
    49141 tataaggata cgtaatgata caacctattt ccggccctcc tcctgggcaa ccaccaggtc
    49201 agggagataa cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt
    49261 cgctggaaag cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt
    49321 gggcgggtat caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact
    49381 tccctgccgc tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg
    49441 cccgccagct tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg
    49501 tcacggatta tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc
    49561 aattaaaaac cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc
    49621 agcgcgaggc gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg
    49681 tgaccaaact tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg
    49741 aactttccgg ggtgaacgat ggcgagttaa ttccagcgaa actgtttaac catctggtga
    49801 cctggttgca ggcgcgccag acgttaagcc agcaaaatac gccgacgctg gaatcactac
    49861 agatggcgct aaaacaacct ttagatgcca gtgaactggc agcgttatcg gcgtatatcc
    49921 agcaaaaata tggtctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc
    49981 ttaatcagct ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc
    50041 ttaatccttt tccaccgatg atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt
    50101 ggatactgtt agtcgcgatt atcctgatgc tggtctggct ggttcgttag ccccgacgaa
    50161 atgacagtat cgtgacaata attcccagca ccgcagagat cgccccggca agaaataccg
    50221 aagagtaacc aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga
    50281 tatcctgaaa cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt
    50341 taaccacctc cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg
    50401 cgcccgctaa tgcgacccac gcacctgggg cttgccagag cagcaacaag cccaccgttt
    50461 ctacaagcag agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa
    50521 acatgacgcg catcacgaca aatgcgccgc caaactcggt aagagtaaag cccgccatcg
    50581 cccatccttt gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc
    50641 cttgtagtgc cagccctaac cctggtttcc agataaaccc gacaacgctc cacagcgatg
    50701 gacgttctcc cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta
    50761 agggtaatac cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca
    50821 ggccaagcgg agcaccaaca gcgagggcac cgtaaatcgc cattccattc catgacatca
    50881 ctttgccaga gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta
    50941 actggctttc accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg
    51001 gtgcggagac aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta
    51061 acatcccctg aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcatagc
    51121 cacgcgtcag caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc
    51181 catagcccag ttcatgatga acaaacagcg ggataaccgg caacggcagc cctacggtca
    51241 tgtaggtgag aaaaaccgca aaagcgatgc ggaagagcga aaaattggca gaagatcgtg
    51301 tttcggtttg gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta
    51361 tccccgtctc atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg
    51421 tcttttcaga aggaaatcga caaagcggga agtttgcctg gaactggtgg cgattgtcaa
    51481 tgatgtgaaa aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc
    51541 gcgtaacgtc ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat
    51601 taatctttca gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta
    51661 gacataacag tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc
    51721 tgctcgtcca gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc
    51781 agagagtaga tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg
    51841 gttgcagaaa tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc
    51901 acgtcgccaa ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg
    51961 ccatgcatcg ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct
    52021 gcaccatcag aggttgcgcc gtagccaacg atttcagcat agatgtgagc accacgcgcc
    52081 agcgcgtgtt ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg
    52141 tcacggtgag cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac
    52201 agcgcaccca tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca
    52261 gcaaacacga tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt
    52321 gcggaagtcg cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg
    52381 gcgaggcagg cagaaacgcc ggatgccatc gctttggtga ccacatacgg gccaaccgct
    52441 ttcaggccgc gcgggccgcg cattgcgtca gcgccgaata cctggaaacg cggggaaccg
    52501 ccgccagaac ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagaaagg
    52561 cccgcatctg cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata
    52621 aagcgcacaa ctttgcggtc aatgaggcca gtggtatcca gttttacgtt gccccagacg
    52681 tggctacgca tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca
    52741 cgcagagatg ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca
    52801 gtaatcactg cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat
    52861 gcacaatagc gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt
    52921 gcgcagcctt acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt
    52981 gaaacactac tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc
    53041 ccgagatttt gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt
    53101 ttttctggga ggcaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt
    53161 ggtagcagag agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga
    53221 tcagtttcgc gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga
    53281 gaaatttccc ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc
    53341 tccgtgggca gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt
    53401 attgctcaat gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac
    53461 cagccaactg gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc
    53521 gccagctaaa aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc
    53581 gcgtccaggc ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca
    53641 ggaagccgga ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg
    53701 tggggtgatg gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg
    53761 cagcagcaaa cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct
    53821 ggcgttatta cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct
    53881 gggtgcttcc ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc
    53941 gctaaaccgc tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc
    54001 cgttaaattt gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca
    54061 gcataaaatc gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga
    54121 ggcaaatgcg gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc
    54181 gcaaggtggt tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca
    54241 gcagggtttg cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg
    54301 ttggttgttg aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa
    54361 cgggcatcaa atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg
    54421 gcaggtcagc catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta
    54481 tgacggttat ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta
    54541 tcatcgcggc agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg
    54601 gttgattgat tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga
    54661 ggcgcgctgc ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc
    54721 cgattatgag gcaacactcg tggaatatgc gtcgttggcg gagcagaaag atgaggcggt
    54781 aagcgcgccg gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg
    54841 ttctgccccg ctgtgtgccg agattctggc ggcgcagatg agcgacgaac cgattccgat
    54901 ggatgccagt acgctgacgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa
    54961 gggtaaagcg gttaaggcgg ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa
    55021 cgccttatcc gacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca
    55081 ggcatggtgc tccagacgcc gcaacttact gctgtgacgc ctgctgaaac aggttttccc
    55141 acatatcggt caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt
    55201 ccagactctg gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca
    55261 gttctgcgat ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat
    55321 cactggcatg gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga
    55381 tcatcttctt tcctcattgt aaaacgtggc accggcttca gttgagcgct tccggccacg
    55441 ggaactgttc cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc
    55501 gtgccggacg ctcgggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc
    55561 gttgaaacac aacacgatcc ggttcgaagt cgccttccag attgtcgcaa ctgacgttaa
    55621 acggatatcc cgccgccaca cagaacaacc agtcgagtgc ctgcggcttc acttcaacat
    55681 cttcaaactg gctttgcgtt tgggcatcac gcccatccgg gcaataccag tagccgaaat
    55741 caaccagctc acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc
    55801 tggcataaaa gccgtgggca aagacaattc ggttatacgg tacttccgca tcagcaggaa
    55861 gatagatcgg ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc
    55921 tattaaaaat ttcaatcaac tgctcatagt ggtgtgtact gttcattagt tcatccccaa
    55981 ccagtggagg atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga
    56041 gacgataaca atcatcgggc ggatcagctt ttgtcctttg ctcaacacca gccgtgaacc
    56101 catgcgcgcg ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt
    56161 gccgccgaga ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc
    56221 gtgagccgtg gctttggcga ggttgaatcc gcacagcgta acgaaggcca gcgcgtaaaa
    56281 cgatccggca gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa
    56341 cgcgaagggt aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag
    56401 caagaaatag aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc
    56461 ctgaacgtac tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt
    56521 gagtttctga tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat
    56581 agagccgccg caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc
    56641 agccatcaat gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc
    56701 cgccagcatg gcgacaaaaa agagaactcc cagcaacagc ggggaaacca taaacaggct
    56761 attaaacgtt tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac
    56821 ggaggcggtg tcttcttctc aggctttgtt gttcccggtt ttagaggtgc aaaccagctt
    56881 tgcagttctg ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga
    56941 ctatcggcag ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc
    57001 actttgcgca accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc
    57061 ggattaacaa aaatgcgcgt gacgtctttg tcctgggcag cgagtttgat caagctgaaa
    57121 atttctggct tccacagcgt ggagacaacg tgtttaccgt cgcgggaaac taagtccagt
    57181 gcttgcgggc gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata
    57241 tcgacatcca gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc
    57301 atccccatat cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga
    57361 cgctggataa acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg
    57421 acctgataat gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa
    57481 aaactgccta tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc
    57541 gctgccaggc tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt
    57601 ttttaccagc gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg
    57661 tgatccatta aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca
    57721 cagggatcgt gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg
    57781 cgccccggca cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc
    57841 ccgctgctga taccgccgag aatgccgccc gcatggttgc tctggaaacc gtctttggtg
    57901 atttcgtcgc ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc
    57961 acgcctttca ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca
    58021 aagaccggct cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg
    58081 atggagtcgc cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcc
    58141 gggtccgggc agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga
    58201 atgtcgccca tctgggtcaa gcagccgcgg atttcaatac caaatttctc ggcgagatat
    58261 tttttggcaa tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca
    58321 ccgccgcgat aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctggg
    58381 cggaaaacgt ctttaattgc gctgtaatcc tgagagcgct ggtcagtgtt ttcgatcaac
    58441 aagccaatgc tggtgccagt agtaacgcct tcaaaaacac cggagagaat tttgacctga
    58501 tccggctcgc ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg gtcgaggtca
    58561 tgttgcaggt ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg
    58621 agcgccagcc catgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt
    58681 ccagccatca cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc
    58741 gaaatattca cgggccgcca gcagctgctc tttggtgagc ataaacacac catcgccgcc
    58801 gttatcaaac tccagccagg tgaacggaac atccggatat tgttccataa gatgtaccat
    58861 gctgttgccg acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgtttcc
    58921 aagaatgcga cgcgtcagtt taaggccgtc ggtgccagat gccaggccca gttccggctc
    58981 gtgacggtat tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt
    59041 gacaatcaga tcgtactgca ctttcggcaa atcacggaac agatcggaac gaatcggaat
    59101 gacgttgtgg atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg
    59161 agagatatct accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat
    59221 gcagccgcta ccggtacaca tatctaaaat atgctgcggt tgcttgctaa tgagtccggc
    59281 aaatttattg ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac
    59341 gtaaaattca tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc
    59401 gttgacgcgg cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc
    59461 ggtgcgcata tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac
    59521 ggcttcatcc cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg
    59581 gcttaccgac cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac
    59641 gaaaatttta tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg
    59701 ccatgaagat gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa
    59761 acgatcgatt gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa
    59821 atgaaaaaga aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg
    59881 gggactcgca agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc
    59941 gaagtgccgg tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat
    60001 gagtttcagc cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc
    60061 cattttgagg cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta
    60121 cacggtctga cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc
    60181 cgcgaacatg tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa
    60241 caaacaccgc tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa
    60301 gagtacggcg gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg
    60361 gagttgccct gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac
    60421 aaatatgtga taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga
    60481 gcaatactac gaactacagt tcattcaata catactgaaa acgacacgtc gaatcaatcg
    60541 gttgcccact actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg
    60601 gatgacgagt tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta
    60661 acaattctga taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc
    60721 catcacagag tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc
    60781 ccgtggactg aattcgtaac gccttattat ggctgacata attttcactc caggagatat
    60841 cgtatgaagt gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc
    60901 ttccaaatcc gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat
    60961 catcagatgc aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta
    61021 tgccagggca atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg
    61081 aacccgaccc agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat
    61141 atttcccata tgtaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta
    61201 cagcaccggt taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca
    61261 taagtttatc tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta
    61321 ggctcgcgaa aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact
    61381 gtggcactaa agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta
    61441 aaatttaggc tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt
    61501 ggcaccgatg tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag
    61561 gagtcaaagg ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg
    61621 acattaaaca atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct
    61681 gactgcccaa ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac
    61741 tggctaccat taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa
    61801 ttatctcctg ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac
    61861 gtacgctttt tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct
    61921 ctggcagaac gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt
    61981 cattcaccat aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc
    62041 ctaaaccagt gatattgctg cttactgcag cattatcaaa atcgcttagt gtgccactcc
    62101 agctcaaggt catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg
    62161 gcacggtttg ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta
    62221 tttcaatggt ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa
    62281 aagagacgtt tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt
    62341 tcattggtaa tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt
    62401 accactctgt ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg
    62461 caaccatgag ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat
    62521 accaaaccca ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc
    62581 ctgcatctgt agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat
    62641 gggacgttta taatcaaccc cattgatctt attaatgaat acattaccaa actcaactgc
    62701 cgcaccattt accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac
    62761 tgacttactc gctgcaactg caaagccgct accaacacaa catatcagta acccagttac
    62821 cgcccagaca gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct
    62881 gctcttcatc gacctggcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc
    62941 catagtcgtt gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcag
    63001 aggttttcgg cgacatcact attggcgaga aaccggctgc aggattgcca tttttctgtg
    63061 tgctggcatt actgaaaatg acgtaatacg gcgtcgggtt attgacggta aattcctgac
    63121 cgttgcgggt taaagtaacc ttatattgcc aggggttttt catgctgacg ttctccagcg
    63181 ccttcggtcg ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt
    63241 tgtttgattt tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag
    63301 gcaatttgtt gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga
    63361 tgcgttgcac cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt
    63421 cgatccagct ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga
    63481 ttgatttgtc gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg
    63541 cgacaaacta gcagcgctaa gcagtaacgc tagcgtcgta gcacccaatt tggcagagca
    63601 aagtaaatca gacatggatc attccttaat cacgttattg gttagctgaa acagaaacag
    63661 ggggattggt gggtgtcacc tgttgcgtct gctcctgaat gacaggcttt atgtcatcag
    63721 gaacaaccgg cgcgacgttg cctttatgct ggcatggcag taacaagccg ttaaacagat
    63781 cggcaggcag cgggtccggc aggttgatat cgcaatgcgc aacaccactc cagaacacac
    63841 tcatgtgttc gccaggtttc accccagcca gataaacatt gccgtcatca tcgacaaggc
    63901 ccactgtctg ctcgttatca ttttttactt ctgcgccaaa cggaggatgg ctgccgtctt
    63961 gtaaacgcag caccgccatc gctttttgac cactgatgac ggcaaatttg cggtagccaa
    64021 tcgccccttc agttagcgtg gcttgtacca ccgactgggt tgcttcggcg ttttccggca
    64081 gtttgttgag gtcgatatac gcctgattgc ggtaatagtt attgacgtca gaaacgacgg
    64141 ctttaccaaa catattggta taaacagcag caccgttacc ttctaccgga acatcggcaa
    64201 cgccatcggc atcaatcaac aagcgtgtcc cgcccatatt ctgggtacgg tgaagtgcgc
    64261 caccgtgggc agtaagcgtt gcgccgccct gtaacgacaa gcccgcagag gtgtattgcc
    64321 cttcatggta gttcgcactg aggtcaacct gcgccagcga accatcatgg ctgtaatagc
    64381 cgtcaacgct ggtgtgtttg tcactggtgc cgacgttcaa ctgatagtga gtcgcgtcat
    64441 cgacacggct gaaataaccg acctgactgc tgtccgtccc actgccatag ttaccgttat
    64501 agctaacggt actgttgtca ccccacggca tactgagcga aatgtacatg cctttgtcgg
    64561 cctggttgtc atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc
    64621 ccatattgaa atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt
    64681 aggtatggcg ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat
    64741 aagtggcagt gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat
    64801 ccagatactc gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa
    64861 cgcggctgtt gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca
    64921 gcgaaccttt gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa
    64981 acgcgaccgc gccgaatgta gacaaatcgc gaccgacgcc aagcgccgca gactgatagt
    65041 tttcatctcc cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt
    65101 cagcaccaga aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca
    65161 tgatcttata gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt
    65221 catattcctg cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag
    65281 aatcaccaag atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac
    65341 gccccatctg gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg
    65401 cgtagccgcg cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat
    65461 agttaaaacc atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt
    65521 ttaatgatgg taacgcccgc taggcatagt agcgactcca ctcccatttt ttttgagttt
    65581 catcgccgct gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt
    65641 cagcacgcat acgccacggc cccaggttaa ccccgaccgt cccgttgccg ctgatctcgt
    65701 tactatcatc accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga
    65761 cgatcccgga gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt
    65821 cgaggtaagc ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt
    65881 ccatgccttc cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt
    65941 tcgccacgtc ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc
    66001 ttttgctcgc gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct
    66061 gtttatttag ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa
    66121 aacgcttcag atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa
    66181 tgtcgtcttc agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg
    66241 gaagaatccg taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt
    66301 cttgttcggt caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt
    66361 acaggtaagt aatctggaag gtggtatttg cttcgaaatt acccagatct ggcgcatcag
    66421 ctgcgccgac cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttattggtag
    66481 aagtgtcttt cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag
    66541 agtcaccgat cgccaggcta acattgttaa atgccgcaca ggtatcggta ttgaaaatgg
    66601 tgtaataatt gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt
    66661 cctgagtatc aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc
    66721 cgttattttt cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt
    66781 ccggaacgat agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt
    66841 tacccgcagc gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag
    66901 ctgttttaac aaactt
//
LOCUS       DAZPJP010000003        64587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPJP010000003 DAZPJP010000000
VERSION     DAZPJP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.003"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHU9J_000141"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHU9J_000141"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1903805.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHU9J_000142"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHU9J_000142"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1903806.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHU9J_000143"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHU9J_000143"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1903807.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHU9J_000144"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHU9J_000144"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1903808.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHU9J_000145"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHU9J_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1903809.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHU9J_000146"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHU9J_000146"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1903810.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHU9J_000147"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHU9J_000147"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1903811.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHU9J_000148"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHU9J_000148"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1903812.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHU9J_000149"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHU9J_000149"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1903813.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHU9J_000150"
     CDS             complement(12799..14139)
                     /locus_tag="ACHU9J_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1903814.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHU9J_000151"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHU9J_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1903815.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHU9J_000152"
     CDS             complement(15928..16377)
                     /locus_tag="ACHU9J_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1903816.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHU9J_000153"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHU9J_000153"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1903817.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHU9J_000154"
     CDS             complement(17177..17506)
                     /locus_tag="ACHU9J_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1903818.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHU9J_000155"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHU9J_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1903819.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHU9J_000156"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHU9J_000156"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1903820.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHU9J_000157"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHU9J_000157"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1903821.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHU9J_000158"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHU9J_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1903822.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHU9J_000159"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHU9J_000159"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1903823.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="ACHU9J_000160"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="ACHU9J_000160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1903824.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHU9J_000161"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHU9J_000161"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1903825.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHU9J_000162"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHU9J_000162"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1903826.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="ACHU9J_000163"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="ACHU9J_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1903827.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="ACHU9J_000164"
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="ACHU9J_000164"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1903828.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="ACHU9J_000165"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="ACHU9J_000165"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1903829.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="ACHU9J_000166"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="ACHU9J_000166"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1903830.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="ACHU9J_000167"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="ACHU9J_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1903831.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHU9J_000168"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHU9J_000168"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1903832.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="ACHU9J_000169"
     CDS             complement(34678..35073)
                     /locus_tag="ACHU9J_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1903833.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHU9J_000170"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHU9J_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1903834.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36360..36587)
                     /locus_tag="ACHU9J_000171"
     CDS             complement(36360..36587)
                     /locus_tag="ACHU9J_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1903835.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36779..37984
                     /gene="csdA"
                     /locus_tag="ACHU9J_000172"
     CDS             36779..37984
                     /gene="csdA"
                     /locus_tag="ACHU9J_000172"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1903836.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37984..38427
                     /gene="csdE"
                     /locus_tag="ACHU9J_000173"
     CDS             37984..38427
                     /gene="csdE"
                     /locus_tag="ACHU9J_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1903837.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHU9J_000174"
     CDS             complement(38478..39284)
                     /gene="tcdA"
                     /locus_tag="ACHU9J_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1903838.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHU9J_000175"
     CDS             complement(39361..40458)
                     /gene="mltA"
                     /locus_tag="ACHU9J_000175"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1903839.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40667..40743
                     /locus_tag="ACHU9J_000176"
     tRNA            40667..40743
                     /locus_tag="ACHU9J_000176"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40701..40703,aa:Met,seq:cat)
     gene            complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHU9J_000177"
     CDS             complement(40818..42071)
                     /gene="amiC"
                     /locus_tag="ACHU9J_000177"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1903840.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42303..43634
                     /gene="argA"
                     /locus_tag="ACHU9J_000178"
     CDS             42303..43634
                     /gene="argA"
                     /locus_tag="ACHU9J_000178"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1903841.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHU9J_000179"
     CDS             complement(43696..45522)
                     /gene="recD"
                     /locus_tag="ACHU9J_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1903842.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHU9J_000180"
     CDS             complement(45522..49064)
                     /gene="recB"
                     /locus_tag="ACHU9J_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1903843.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49057..51940)
                     /gene="ptrA"
                     /locus_tag="ACHU9J_000181"
                     /pseudo
     CDS             complement(49057..51940)
                     /gene="ptrA"
                     /locus_tag="ACHU9J_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
     gene            complement(52116..55484)
                     /gene="recC"
                     /locus_tag="ACHU9J_000182"
     CDS             complement(52116..55484)
                     /gene="recC"
                     /locus_tag="ACHU9J_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1903844.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55497..55820)
                     /locus_tag="ACHU9J_000183"
     CDS             complement(55497..55820)
                     /locus_tag="ACHU9J_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1903845.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55805..56212)
                     /locus_tag="ACHU9J_000184"
     CDS             complement(55805..56212)
                     /locus_tag="ACHU9J_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1903846.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56209..56772)
                     /locus_tag="ACHU9J_000185"
     CDS             complement(56209..56772)
                     /locus_tag="ACHU9J_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1903847.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56763..57233)
                     /locus_tag="ACHU9J_000186"
                     /pseudo
     CDS             complement(56763..57233)
                     /locus_tag="ACHU9J_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57418..58212)
                     /gene="thyA"
                     /locus_tag="ACHU9J_000187"
     CDS             complement(57418..58212)
                     /gene="thyA"
                     /locus_tag="ACHU9J_000187"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1903848.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58219..59094)
                     /gene="lgt"
                     /locus_tag="ACHU9J_000188"
     CDS             complement(58219..59094)
                     /gene="lgt"
                     /locus_tag="ACHU9J_000188"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1903849.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59245..61491)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000189"
     CDS             complement(59245..61491)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000189"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1903850.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61504..62034)
                     /gene="rppH"
                     /locus_tag="ACHU9J_000190"
     CDS             complement(61504..62034)
                     /gene="rppH"
                     /locus_tag="ACHU9J_000190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1903851.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62387..62533)
                     /gene="ygdT"
                     /locus_tag="ACHU9J_000191"
     CDS             complement(62387..62533)
                     /gene="ygdT"
                     /locus_tag="ACHU9J_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1903852.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62719..63408
                     /gene="mutH"
                     /locus_tag="ACHU9J_000192"
     CDS             62719..63408
                     /gene="mutH"
                     /locus_tag="ACHU9J_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1903853.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63477..64190
                     /locus_tag="ACHU9J_000193"
     CDS             63477..64190
                     /locus_tag="ACHU9J_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1903854.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64328..64546
                     /gene="ygdR"
                     /locus_tag="ACHU9J_000194"
     CDS             64328..64546
                     /gene="ygdR"
                     /locus_tag="ACHU9J_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1903855.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15656 a        17364 c        15824 g        15743 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcacc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 gcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcaagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaggcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg aaacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt caaatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacgcgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgccgcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagc gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccggg ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgtcacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 tcttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attctaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagccaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgggcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc actttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtta aattaattta acaattagat cacactgtgt
    36061 aacctattag tttttttaat ctgagccatt ataaattgtc cgttgagctt ctaccagcaa
    36121 atacctatag tggcggcact tcctgagccg gaacgaaaag ttttatcgga atgcgtgttc
    36181 tggtgaactt ttggcttacg gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga
    36241 ttcagaccat ggtagcaaag ctaccttttt tcacttcctg tacatttacc ctgtctgtcc
    36301 atagtgatta atgtagcacc gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat
    36361 tactgatcca gttcgaccat ctctttcacg tcagtacggt tgatctgctg tttgttgcca
    36421 ttagcgtctt tatacgaaat cataccggta tcgttatcag tctgtggttt gccgtcagag
    36481 acgatggtac gtccgtcatt ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg
    36541 gcaaaagtca gcatacaggc agaaataatt gcggcagtct ttttcatcat cttctcctta
    36601 aagcgattaa taaatcttaa tagtattgat taacaggcta aaattaacgc ctaacactat
    36661 tcagcatatg ttacttacgc ggcttcgcca ggatatccag ataattctga tggttagcac
    36721 tctccttgta tcaaagtgaa ttttgcgtca cgatcggtgc atcaagccga ggagtaccat
    36781 gaacgttttt aatcccgcgc agtttcgcgc ccagtttccc gcactacagg atgcgggcgt
    36841 ctatctcgat agcgccgcga ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca
    36901 gttttacagc ctgagcgccg gaaacgtcca tcgcagccag tttgccgaag cccaacgcct
    36961 gaccgcgcgt tatgaagctg cacgagagaa agtggcgcaa ttactgaatg caccggatga
    37021 taaaactatc gtctggacgc gcggcaccac tgaatccatc aacatggtgg cacaatgcta
    37081 tgcgcgtccg cgtctgcaac cgggcgatga gattattgtc agcgtggcag aacaccacgc
    37141 taacctcgtc ccctggctga tggtcgccca acaaactgga gccaaagtgg tgaaattgcc
    37201 gcttaatgcg cagcgactgc cggatgtcga tttgttgcca gaactgatta ctccccgtag
    37261 tcggattctg gcgttgggtc agatgtcgaa cgttactggc ggttgcccgg atctggcgcg
    37321 agcgattacc tttgctcatt cagccgggat ggtggtgatg gttgatggtg ctcagggggc
    37381 ggtgcatttc cccgcagatg ttcagcaact agatatcgat ttctatgctt tctctggtca
    37441 caaactgtat ggcccgacgg gtatcggcgt gctgtatggc aaatcagaac tgctggaagc
    37501 gatgtcgccc tggctgggtg gcggcaaaat gattcacgaa gtgagttttg acggcttcac
    37561 gactcaatct gcgccgtgga aactggaagc aggaacgcca aatgtggctg gtgtcatagg
    37621 attaagcgcg gcgctggaat ggctggcaga ttacgatatc aaccaggcag aaagttggag
    37681 tcgcagcctg gcgacgctgg cggaagatgc gctggcaaaa cgtcctggct tccgctcatt
    37741 ccgctgccag gattccagcc tgctggcctt tgattttgct ggcgttcatc acagcgatat
    37801 ggtgacgctg ctggcggagt acggtattgc cctgcgggcc gggcaacatt gcgctcagcc
    37861 gctactggca gaattaggcg taaccggcac actgcgcgcc tcttttgcgc catataatac
    37921 aaagagtgat gtggatgcgc tggtgaatgc cgttgaccgc gcgctggaat tattggtgga
    37981 ttaatgacga acccgcaatt cgccggacat ccgttcggca caaccgtaac cgcagaaacg
    38041 ttacgcaata ccttcgcacc gttgtcgcaa tgggaagata aatatcgcca gttgatcatg
    38101 ctggggaaac agcttccggc tttgccagac gagttaaaag cgcaggctaa agagattgcc
    38161 ggatgcgaaa accgcgtctg gctgggatat accgtgactg aaaacggtaa aatgcacttc
    38221 tttggcgata gcgaagggcg cattgtgcgc ggcctgctgg cggtgctgtt gactgccgtt
    38281 gaggggaaaa ccgccgccga attgcaggca cagtcgccgc tggcattgtt tgatgagctg
    38341 ggattgcgtg cgcaacttag cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg
    38401 attatcgctg cgacgaagca ggtttaagca tagaaccggg tcggataaga cgttcgcgtc
    38461 tcatccgacc tgattgttta accctgacgc gccgctttcg ccatcatctt cttcagcgca
    38521 tgagaaaccg caacaaaccc aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat
    38581 gcacaatcca tccgcttcgg cccttctgcc gtggctttca tcgcacacac agtaccgtct
    38641 gactgcgggt acaccagcgc ttcagtagaa aacacgcaat cgacgccgag cttaccttta
    38701 ctgtttttca ctacgccaaa atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga
    38761 tcctgaatcg ttttcgccag atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc
    38821 gcgccaccgg tcgtgaccag cgggatttta ttgcgccgac aataagcaat cagcgccgct
    38881 ttgggccgta cactatcaat ggcatcaatc acgtacgaat aacccacgct catatactgc
    38941 gctacgttat ccggcgtcac gaaatcatcc accaccgtta cacggcactc cgggttaatc
    39001 tggcgaatac gctccgccat gacttccgct tttgccagcc cgacgttatc gcgcagagcg
    39061 tgaatttgcc gattggtatt ggtgacgcac acatcatcca tatcgataag cgtgattgcg
    39121 ccaatccccg tgcgcgccag cgcttccgcc gcccaggaac cgacaccacc gataccaacc
    39181 acacaaatat gcgcgtcagc aaacagttgc aacgcttttt caccatacag acgcgctgtg
    39241 ccaccaaaac gctgacgcca tgcatcacta attaccacag acatagcacc tcagaattaa
    39301 aaagggtgag gttatccctc accctgtcgt atccgtaaaa acggtataca gaataacaca
    39361 tcagccgcta aagacgttac ctgcgcccgg ggcggttttc agcacccaga cacgtccata
    39421 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    39481 gtgttggcct ttgattgcac caccgacatc cagcgccacc atcagacgca gttcgtactg
    39541 accattaaat ttgccgttat tatccagcaa cggcacttct gccagcaagg tagtacctgg
    39601 cggaataatg gaacgatcag aggcaactga cgcgcgacca accagcggca ccgcacttgc
    39661 ccctttcacc ggtgcaaaag attgcggttt aaagaagacg aaagacgggt tctgttccag
    39721 cagctcgcga acctcggctt cactgtgtgt ttcgccccag tgacgaatcg cctgcatcga
    39781 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    39841 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    39901 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    39961 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    40021 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    40081 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    40141 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    40201 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    40261 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    40321 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    40381 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    40441 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    40501 acaaaccgcc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    40561 ataggcttta gtgatttgtt tttgttcaaa atcatgccag atccgtgatc ggggtaaaaa
    40621 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggaggcg gggtggagca
    40681 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    40741 ccaattattg aacaccctaa cgggtgtttt tttgtttcta ctctcccata aaaaaagcgc
    40801 cattcagcgc ctttttatca tccccttctc gccagcgttg ccccatcggc aaaatacgct
    40861 ttaatccccg caagaataga ctccgcaact tcctgctgga aagtcgccgt tttcagttta
    40921 cgctcttcct caacgttact gataaacgcc gtttcgacca gaatggaggg aatatctggt
    40981 gcctttagta cggcaaaccc ggcctgttca acttgatttt tatgcagctt gttgatttta
    41041 ccgagcttat tcagcactgc tttaccaaac ttaaggctgt cagcaatggt cagcgactgc
    41101 accatatcga acatggtgtg gtcgacatag cggtcaccgc ttttgctcac gccaccaatc
    41161 aagtccgagg cgttctgggt ttgtgccaga tattttgccg cagtactggt tgcacctttg
    41221 gttgagagcg caaacaccga ggaaccgctc ggctggcgac tggtaaaggc gtcggcatgg
    41281 atagagacaa acaggtcagc acgctgtttc tgggcttttg ctacgcgcac ttgcaacgga
    41341 atgaagatgt cttcattgcg cgtcatgtac accttcatat tgccctcttt ctcgatcaga
    41401 gagcgcaaac ggcgagctat ttgcaatacc acatcttttt cgcgcgtttt gtatttcccc
    41461 accgcaccgg agtcttcgcc accgtggcca gggtcaagca taatgacaat cggacgatcg
    41521 cgccctgctt tacctggttg tggaccactt tgcgctggcg gcacctgctt ttcgaggtcg
    41581 cctttgttgt aatcctccag cagcgccagc agcgggtcct gcatatcctg tgcattggcc
    41641 ggatagaggt ccatcaccag acgctcttta aacccggcga ccggcgcaag ggcaaacagc
    41701 tgcggtttta cgttttgctt taattcaaaa accatccgta cggtttgcgg gtcaaattgc
    41761 ccgacgcgcg ccgacttgat gaacgggtcg tctgcacgaa tttgtgccgc catccccttg
    41821 agtaccgagt tcaggtttac atcttcgata tccaccacca cgcgttcagg gttactcaac
    41881 gcgaactgct tatatttcag ctgacgattt gattctaccg tcacgcgggt gtagctggac
    41941 gcaggccaga cgcgcaccgc cacgacctgg ctaaccgcag ccaggctgac ctgacttacg
    42001 ctcaatagcc acatagcgcc cgcgccttgc agtaaacgac gacggctgat tgcagtgttg
    42061 gatcctgaca tgcctctccc gagcaaaaga aatctaagct gtgtaacaag taaacgacta
    42121 atttgaccgg aaactttagc gaaagacgca taatctgtca tctaataaac ggtaaacatt
    42181 ctttttatat tcacggcatt actgataaaa aagtcgctct cgcataaaat ttacacttgc
    42241 accctgcgaa aaaacagaat aaaaatacaa taatttcgaa taatcatgca aaggggtgtg
    42301 ccgtggtaaa ggaacgtaaa accgagctgg tcgagggatt ccgccattcg gttccctata
    42361 tcaataccca ccggggaaaa acgtttgtca tcatgctcgg cggtgaagcc attgagcatg
    42421 agaatttctc cagtatcgtt aatgatatcg ggttgttgca cagcctcggc atccgtctgg
    42481 tggtggtcta tggcgcacgt ccgcagatcg acgcaaatct ggctgcgcat caccacgaac
    42541 cgctgtatca caagaatata cgtgtgaccg acgccaaaac actggaactg gtgaagcagg
    42601 ctgcgggaac attgcaactg gatattactg ctcgcctgtc gatgagtctc aataacacgc
    42661 cgctgcaggg cgcgcatatc aacgtcgtca gtggcaattt tattattgcc cagccgctgg
    42721 gcgtcgatga cggcgtggat tactgccata gcgggcgtat ccggcggatt gatgaagacg
    42781 cgatccgtcg tcaactggac agcggtgcaa tagtgctaat ggggccggtc gctgtttcag
    42841 tcactggcga gagctttaat ctgacctcgg aagagattgc cactcaactg gccatcaaac
    42901 tgaaagctga gaagatgatt ggtttttgct cttcacaggg cgtcactaat gacgacggtg
    42961 atattgtctc agaacttttc cctaacgaag cgcaagcgcg ggtagaagcc caggaagaga
    43021 aaggcgatta caactccggt acggtgcgct ttttgcgtgg cgcagtgaaa gcctgccgca
    43081 gcggcgtgcg tcgctgtcat ttaatcagtt atcaggaaga tggcgcgctg ttgcaagagt
    43141 tgttctcacg cgacggtatc ggtacgcaga ttgtgatgga aagcgccgag cagattcgtc
    43201 gcgcaacaat caacgatatt ggcggtattc tggagttgat tcgcccgctg gagcagcaag
    43261 gtattctggt gcgccgttct cgcgagcagc tggagatgga aatcgacaaa ttcaccatta
    43321 ttcagctcga taacacgact attgcctgcg ccgcgctcta tccgttcccg gaagagaaga
    43381 ttggggaaat ggcctgtgtg gcagttcacc cggattaccg cagttcatca cggggcgagg
    43441 ttctgctgga acgcattgcc gctcaggcga agcagagcgg cttaagcaaa ttgtttgtgc
    43501 tgaccacgcg cagtattcac tggttccagg aacgtggatt taccccggtg gatattgatt
    43561 tactgcccga gagcaaaaag cagttgtaca actaccagcg taaatccaaa gtgttgatgg
    43621 cggatttagg gtaagcgatg aaaatcgccg gatgcggcag gcgtaacact cgtgcgtcgc
    43681 atccggcaat tacgtttatc cccgcgaact aaacaacgcc gccagaccac tgcgccgctc
    43741 agtacgagtg gcgattgccg cacttaatat acgctcatcg gcatacagcg acagacggcg
    43801 acgcgcgcgg gtcaccgcgg tgtataccag ctctcgcgtt actatcggcg tgcgttggct
    43861 cggtaaaatc aacgccgcat ggtcgaactc cgatccctgc gatttatgta ccgtcatcgc
    43921 ccacgtagtt tcgtgctctg gcaagcgact cggttgcaca gacttaatat tgccgtccgg
    43981 catcgcaaac cagacgcgcg tcccctgccc gcgatccagc gcaataccga tatcgccatt
    44041 aaacaaccca agcgcactgt cattacgggc aatcatcacc ggtcgacctt cgtaccaacg
    44101 agagtgcgaa tgacgatgaa ttttgcgctt ctgttgcata aactgctcaa ttcgctcatt
    44161 cagtccagcc acgccaaatg gcccttcccg cagggcgcac aaaagttggt actcattgaa
    44221 cgcctgaatg attaaatccg gctcggcacg cgcttgcagc agatccagat aacgtccata
    44281 acccgcaaga gcttcctcaa gcatcgcaat ataatcttcg ccgctctgta aaagccgttt
    44341 ttcaatatca gtaaaatcct gctgaaaaac ggttttcact gccgttttat caccacggtt
    44401 gatcgccgca gctaactgac caatgccaga atcgctgccg aaacgatagc ttttttgcag
    44461 caggcagaga ctgtcgcgca aagatgccgc ttctgtgccg gttcctgccg gaacgtgagt
    44521 tcccgtcagg cggcttagct gcccggcacg ctcggcggta aagccggcat tggcataagc
    44581 gcagatatcg cccagcacag ccccagcctc aaccgaggcc agttgatcac gatcgccgag
    44641 aaagatcact cgcgcatgat cgggcaaggc gtcgatcagt ctcgacatca taggcagatc
    44701 gatcattgac gcttcatcta ccaccagcac atcaagatgc agcgggttac cggcatgatg
    44761 acgtaaacgc tggctacccg gctgcgcgcc cagcaatcgg tgcaaagtgc tggcatcttc
    44821 cggaatgcgt ttcttttgtt catcggtcag cggtaactgt cgcaaagcct tgccgagaga
    44881 ttcggttaag cgcgcggcag ctttacccgt tggtgcagcc agacggatac ggcagcgttc
    44941 gccgtcggcc atttgaatta acgctgccag caacttcgcc acggtggtcg ttttaccggt
    45001 gcctgggccg ccggaaatca ccgagatccg ccgcgtcagc gccactgctg ccgccacttt
    45061 ttgccagtta atttcatcgc ttactggaaa aagtttgtcc agggtttgcg ccagtagagc
    45121 ttcatcaacc tcaatggcat gattcacctc attgaagaag cgcgctaccg tccgctcgtt
    45181 acaccacatg cgattcaaat aaagacgatc gccacagagg atcatcggcg tgggttcatc
    45241 tcccctgctg accgcttgag aagccagcaa gcattcttcc caattttgta gctcaccgat
    45301 ttcactgaca caggtcgcca acagcggatg cgacgcctcg ttattttcca gtcgtgaaag
    45361 cggcaaacag acgtgtccct ctccggcatc atgacttaac agtgccgccg cgagggtgac
    45421 ggcaggatgt tcatctcccg ccacggtcag ggcaaattgc acatccagcg ggcgtagctg
    45481 tttgtgctcc acagcttcca gtaattgctt ttgcaatttc attacgcctc ctccagggtc
    45541 ataccggcaa acatctcatc catcagggca atcaacccgg cgttgggtcg ggttgtgtaa
    45601 atcccttgtt gcggatgttc tttatcaacg ccacgcagga acagatagat aacgccgcca
    45661 aagtgacgat catagtcgta atcagcaatg cgatggcgca gataacgatg cagcgccagg
    45721 gtataaagct gatattgcag atcatagcgg tgtgcctgca tcgctgctgc catagcctgt
    45781 tgggtgtaag ccgaactgtc ttcacccagc cagttggatt tatagtcgag caggtaataa
    45841 cgcccttcgt ggcggaatac caggtcgata aagcctttta acatgccgcg tacctgcatg
    45901 aactccagcg gcgggcagcc agcggatagc gggtcaaact ggcgaattag tgcatcaagc
    45961 tggctggcga tgagcggttc gctaatcggc aggtaaaact ccatctccac ctgtttatcg
    46021 cggtcggaaa gctgactcag gctaacaccc gtttcattga gaggtgcctg gaggacagcc
    46081 gtgatccact cggtcaacac cggttcccac tgcgattcaa agccgccgag ttccagtttt
    46141 tcctgtaccc agttcgggtc aaccggctgg gtaaaatcga ggtcttcaaa caaactgtgc
    46201 aagaatgtcc ccggtgacgc accgcgcggg aactgatgcg gtgttaacgt cggttcttca
    46261 acgacgctga ccacgcctgc ggcatcaaca tccagccgtg gcatcagatc ctgagcgata
    46321 ccgtgaccac gctgctgcaa gccggagtag ctggtgacac gccagttatc gccgggcaat
    46381 cgttgtagtg tcctcgcatt cagttctgca gttaaagcat cattaacctg ccagggctgg
    46441 ttatcaccag tttgtgccgt ttgccaggca atatcatcat cgcataacgc ttcaatacag
    46501 gtgcgaagcc ctgccgcatc ttgcggttcc cctttttgca gcaaacgccc gagcgcactt
    46561 tggtggacgt cggtgtcacc ttttttatcg ccacgacggc gcaccagcgg tgcaacgccg
    46621 agactgcaat gccaaaccga acgcgtcagc gccacgtaaa gcaaacgcag atcttccgcc
    46681 agacgttcga cctccgcgag gtcgacgctt tctggcgcag cattaagatc caaaactgcc
    46741 tcaaacgagt ggcgatcgtg ataaaacgcc tgatcctgga cgcggaaatt ggtaataaat
    46801 ggcaaccaga ccagcggata ttccagccct ttcgatttat ggatcgtgac aatctgcacc
    46861 agatgtttat cactttcgag gcgcatttgt tggctggagg cattactgtc tggctcgagg
    46921 atatgttgcg ataaccagcg taccagcgca tgttcacttt ccagctgcgt tccggcttct
    46981 tgaagcagtt cgctgatatg caagatatcg gtaagacgcc gctcaccgcc tgccgttgcc
    47041 agcaagtttt ctgcaatgtt acgcgccgac atcagcgccc gcagcatcgg cataacgcca
    47101 cgtttgcgcc agatttgccg ataaccatcg aactcttcga ctaccgcatc ccacgcatgt
    47161 tcgtcattat tcagcgtttc aatatccagc gcattcagcc ccatcattga cgttgccagc
    47221 gcactgcgca gggtgttctc acgttcgggc gtcatcaccg cctgcaacag ccaaagcatt
    47281 tcctgcgctt ccagagtttc aaaaacacta tcgcggttcg aaaggtaaac ggaagggatt
    47341 tccagcaacg ttaaggcatc gcgcacctgg gcggcctcct ggcggctgcg caccagcacg
    47401 ctgatgtccg aagcacgcac cggacgcgcg tcgtcgccgt tcatcagcaa cgcttcgccc
    47461 cgctgtccgg cttgtagcca gtcgcggatt tgcgcagcac atacctgcgc catggtactt
    47521 tgatagtcgc caacgccgca gctttcgcct tccatcagcc acattttcat cgcaggctgc
    47581 gtttcacctt taaagacaaa acgtaacgcc tgatttttcc cggctgattt cactggaata
    47641 aacggtattt cgcgaaacat gaacgcgtca tcagtctggc tgaaaagctt gttcacactg
    47701 ttcaccattc caggtgcgga gcgccagttg gtgtctaaag tgtagtgggc gtgaacttca
    47761 ctacgcgcct tcatataagt gaagatatcc gcaccccgga atgcatatat ggcctgcttc
    47821 gggtcgccaa tcagcaacaa tgcggtttct ggctgatggt gccagatacg gcgaaaaatt
    47881 cggtactgct gggggtcggt atcctgaaat tcatcgatca ttgccaccgg gaatcgcgta
    47941 cggatcgccg ctgccaacac ctcgccgctt tcgctacgca gcgcggaatc gagccgactt
    48001 aacatgtcat caaaacccaa ttcgccacgg cggcgttttt cacgcgctac tgtttcgcgg
    48061 atctcagcca atgcgcgggt gataaccaga tcacggatcg acaatggttc tgcaagcagt
    48121 tgatcgatcg cctcaaacag tggatgtcgc ggggtttccc ccccggcttt cgtgcgatct
    48181 tctaagaaac gctgggagaa tttttccagc gactccggca actgataact gtttgtctct
    48241 tcttctgccc aggcgctgat cttgtcgatc catttagcct gattgctacg gttaaacttg
    48301 cgtcgatcaa taccagaaga ttcgatcagc gcatccagtt cacccactgc gtcgcgccac
    48361 tgctgtttta ccgtatcaat acgcgccaca atttgcgcgt gacgggaagc tagcgtttca
    48421 tcatcgggcg gcggtgcttt gataaccggc gcttcgcctt gcagataacg attaatatcg
    48481 cgcagcaacg cctgcggccc tttccaggtt tcaaagacga cctgggctat ttcacgcggc
    48541 agcgggtagc agtggcgacg ccagaaatcg gcgcaggcct ggtagcgtag cagagactca
    48601 tcttcaatca gctgctgctc aaacagcatg ccggattcaa aggcattcag gttgagcatg
    48661 cgctggcaaa agccgtgaat agtaaagact gccgcttcat ccatctgccg ttcggctaac
    48721 aacaaccact gcgcggcttg cgctttatcg tcgatctctt ccagcaggtg ttcgtacaat
    48781 ggattgtcgg tggtttcacg cagacaggcg atgcgcaact cgtggatatt gctacggata
    48841 cgaccgcgca attctgccgt ggcagcctcg gtaaaggtga ccaccagcag ttcttcaacg
    48901 gtcagcgggc ggggaaaggc ggcggaaccg cctagtccaa gtaacaggcg caaataaagc
    48961 gccgcaatcg taaaggtttt gcctgtgccg gcagaggctt caatcaggcg ctcgccctgt
    49021 aagggcaagc gcaaaggatc tagtgtctcg gcgacatcac tcattctttt cactcatcag
    49081 gggcattgtt tgctgcaacg cgctgacgtt ctcccacact ttccagcctt caggatgcac
    49141 atattcggct ttcccgtttt ggctgccgga aatctgcgac aggatagcca tgccttgcgg
    49201 ctcgactacc gcctgatgga agaaatcggc cagtttttgc ggcgtcagca gtttaatctg
    49261 ggccacgatt ttatcacgcg aatcgaagcg catattgccg cgatcgaaat ctttacttaa
    49321 cttcgatttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
    49381 tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt gggaaaaacg
    49441 ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca
    49501 tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt
    49561 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
    49621 ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
    49681 tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt
    49741 ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
    49801 ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
    49861 aggcgtcgcg ataagccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt
    49921 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa
    49981 acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
    50041 gctgatcttc cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg
    50101 gcagacgctg ggggtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
    50161 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
    50221 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
    50281 aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca
    50341 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa
    50401 tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg
    50461 cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg
    50521 catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg
    50581 cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg
    50641 caatattgac tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc
    50701 attcgacgta atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat
    50761 tcgccagttc atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc
    50821 taaaaattgc cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag
    50881 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    50941 caactaatcc ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca
    51001 gataggtaat caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa
    51061 actcaacgcg cagaacttta cgcggcattg ccgggacgta atgaatgata atgccctttt
    51121 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    51181 gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa
    51241 ccgccttcat cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    51301 ccggattacc aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaacgg
    51361 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac
    51421 gcgccatggt taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga
    51481 gcaaaggttc agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct
    51541 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    51601 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    51661 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    51721 cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    51781 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    51841 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    51901 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tctcggacct cataatcaac
    51961 ttaattttct gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt
    52021 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    52081 gtaagcggat agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc
    52141 ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc
    52201 cgccagagcc tttgatacca gatatcatca ccttcgccac gcaccatcat gttgccttcg
    52261 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    52321 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    52381 aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    52441 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    52501 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    52561 gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac
    52621 cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg
    52681 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa
    52741 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt
    52801 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    52861 agcccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt acggaagttc
    52921 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    52981 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    53041 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    53101 tgatagtttt gtggatcaaa cggcattcgg gtatggaggc aagtaagatg cgcttttacc
    53161 cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg
    53221 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    53281 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    53341 agatagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    53401 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    53461 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg
    53521 agaaaacgct ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt
    53581 gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    53641 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgctg
    53701 agcatatcgc gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga
    53761 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    53821 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    53881 tagcccagca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    53941 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    54001 tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    54061 tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg
    54121 ataaacgctt ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    54181 aggtaacgat ccgcaggtgc gctaccaaac acagcccgaa taaacggact gtagctgtcg
    54241 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc
    54301 gccagcaggc gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    54361 gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg
    54421 atgttcacac cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc
    54481 agcaggttat ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca
    54541 gaaaggagat aaatgtagtc gcgcccaagc ttaccccatg aagccagcag cgggttgccg
    54601 acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctatc acgaaatagc
    54661 ggcaattcgc gatcttcaaa actgtgtcga cgctgacggg tcagcagttt cgccagataa
    54721 gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    54781 tcaatatgtt tacccagcgc ctgtagcgcc tggagataaa caggcggtaa cgcggaaata
    54841 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    54901 tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgcccgag ttgatgggta
    54961 tattccacca gcgccttcca caacggggct tgccaggcct gtgcttctcc cagcccttca
    55021 accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag atactggtca
    55081 aacaggtccg ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc
    55141 agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag cagagtcatc
    55201 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatttc cggtaacacc
    55261 cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata
    55321 ctaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga
    55381 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    55441 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    55501 tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc
    55561 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    55621 gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg
    55681 tactggtttc tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg
    55741 acaatcatca ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga
    55801 ttcttcaggg aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc
    55861 gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    55921 cataaccggc aaccaataag gcttcattat ctgccagtaa acgcaaacaa acccgggcat
    55981 cggttccagc gtactgcgag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    56041 cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt tctatgctca
    56101 cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa
    56161 ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa
    56221 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    56281 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    56341 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    56401 cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc
    56461 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    56521 gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc
    56581 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag
    56641 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag
    56701 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    56761 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    56821 accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta
    56881 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttagac ttcgggccag
    56941 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc
    57001 acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag
    57061 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc
    57121 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    57181 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    57241 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    57301 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    57361 cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta
    57421 gatagccacc ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc
    57481 gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg
    57541 cggttcgcgg cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtatc
    57601 gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac
    57661 cagtaacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg
    57721 ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg
    57781 tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcggga
    57841 atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc
    57901 tggcgttggc caggcgcgcc actgtttacc atacactggt ccgaggtcgc cgttttcatc
    57961 ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc
    58021 ctgcagaaac cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt tagttgtcac
    58081 cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc
    58141 ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa
    58201 atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac
    58261 catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca tgctgatgta
    58321 ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg
    58381 aaacgcgccg tagccaatca ggaacaagcc agagacagct cccattgggc gcggtttacg
    58441 aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagct cgtaaagctg
    58501 tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt
    58561 ttgcagcagc aaaatatctt ctgtacggga gcctgggaac agcatggcaa acgggaagtt
    58621 cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag
    58681 accaaacgga atgagtggtg cgataaaatc agaaacctgg aagaaggaac gtttagtacg
    58741 gcgggcaaag ataatcatca ccacgataac gccaatcagg ccgccgtgga aagacatgcc
    58801 gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa
    58861 cagaacataa ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat agagtaagtt
    58921 ttcaacttca tttttggtcc agccgctgcc cggacgattc gcccgtcgtg ttgccagcca
    58981 cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc
    59041 tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca
    59101 ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt
    59161 caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt
    59221 taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg
    59281 acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact
    59341 acgctgcgca agattttctg cttcggcata atcaatgcgc cgcagcaggt attttacccg
    59401 cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac
    59461 gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc
    59521 ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt
    59581 cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc
    59641 aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac
    59701 ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc
    59761 acgtccggca cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat
    59821 cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg
    59881 gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg
    59941 attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg
    60001 caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc
    60061 ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat
    60121 gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat
    60181 gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag
    60241 acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc
    60301 cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac
    60361 cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc
    60421 agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag
    60481 ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc
    60541 gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa
    60601 atcgccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt
    60661 ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa
    60721 ttcgcgacgc agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt
    60781 ttctttttgt gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc
    60841 ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata
    60901 cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg
    60961 tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg
    61021 cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact
    61081 ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg
    61141 ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta
    61201 tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac
    61261 caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag
    61321 cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca
    61381 gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag
    61441 gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt
    61501 gacttaacct cttttacgtc gataagcaga tgtgttttgt ggttttggcg tattttcctg
    61561 cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa
    61621 tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg
    61681 tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca
    61741 tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa
    61801 tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc
    61861 tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc
    61921 gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg
    61981 gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact
    62041 acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaagaag acggtaaacc
    62101 actctctgca gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt
    62161 tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt
    62221 gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac
    62281 attttaaaat attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt
    62341 aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt
    62401 atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt
    62461 cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt
    62521 ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat
    62581 tgcagtgttg cgcaactcga ttaccggcag cctaaaaagc aatataaatc agagaattga
    62641 acaacacatg tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt
    62701 taaccaaggt atcatgacat gtcccaacct cgcccactcc tctctcctcc cgaaactgaa
    62761 gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca
    62821 cttgccgggc tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta
    62881 ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg
    62941 ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc
    63001 gtttgtgttg ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc
    63061 cacaagctca aacgcgtact gtggataccg gttgaaggtg agcgcagcat cccgctggcg
    63121 cagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta
    63181 cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc
    63241 gctcgtcacg gggagtattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc
    63301 gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag
    63361 aagaatttca ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga
    63421 cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt
    63481 tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg
    63541 agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca aagctcccca
    63601 cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg
    63661 cgctgctggc atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca
    63721 gtcaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga
    63781 aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg
    63841 tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg
    63901 actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg
    63961 tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc
    64021 atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtcggcttt accctgattc
    64081 tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta
    64141 ttgcggttga aagcctcaac ctgattcgca acaaaaagaa cccgctctga tactccgtac
    64201 gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct
    64261 ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga
    64321 taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc
    64381 cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg
    64441 aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc
    64501 gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc
    64561 atccggctgt aatgccgatc agttaag
//
LOCUS       DAZPJP010000004        62825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPJP010000004 DAZPJP010000000
VERSION     DAZPJP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.004"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            203..652
                     /gene="alaE"
                     /locus_tag="ACHU9J_000195"
     CDS             203..652
                     /gene="alaE"
                     /locus_tag="ACHU9J_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1903856.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(689..1033)
                     /locus_tag="ACHU9J_000196"
     CDS             complement(689..1033)
                     /locus_tag="ACHU9J_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1903857.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1185..1514
                     /locus_tag="ACHU9J_000197"
     CDS             1185..1514
                     /locus_tag="ACHU9J_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1903858.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1762..2007
                     /gene="nrdH"
                     /locus_tag="ACHU9J_000198"
     CDS             1762..2007
                     /gene="nrdH"
                     /locus_tag="ACHU9J_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1903859.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2004..2414
                     /gene="nrdI"
                     /locus_tag="ACHU9J_000199"
                     /pseudo
     CDS             2004..2414
                     /gene="nrdI"
                     /locus_tag="ACHU9J_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2387..4531
                     /gene="nrdE"
                     /locus_tag="ACHU9J_000200"
     CDS             2387..4531
                     /gene="nrdE"
                     /locus_tag="ACHU9J_000200"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1903860.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4541..5500
                     /gene="nrdF"
                     /locus_tag="ACHU9J_000201"
     CDS             4541..5500
                     /gene="nrdF"
                     /locus_tag="ACHU9J_000201"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1903861.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5856..7058
                     /gene="proV"
                     /locus_tag="ACHU9J_000202"
     CDS             5856..7058
                     /gene="proV"
                     /locus_tag="ACHU9J_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1903862.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7051..8115
                     /gene="proW"
                     /locus_tag="ACHU9J_000203"
     CDS             7051..8115
                     /gene="proW"
                     /locus_tag="ACHU9J_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1903863.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8173..9165
                     /gene="proX"
                     /locus_tag="ACHU9J_000204"
     CDS             8173..9165
                     /gene="proX"
                     /locus_tag="ACHU9J_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1903864.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9456..10640
                     /locus_tag="ACHU9J_000205"
     CDS             9456..10640
                     /locus_tag="ACHU9J_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1903865.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10764..11501
                     /gene="ygaZ"
                     /locus_tag="ACHU9J_000206"
     CDS             10764..11501
                     /gene="ygaZ"
                     /locus_tag="ACHU9J_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1903866.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11491..11826
                     /gene="ygaH"
                     /locus_tag="ACHU9J_000207"
     CDS             11491..11826
                     /gene="ygaH"
                     /locus_tag="ACHU9J_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1903867.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11917..12447
                     /gene="emrR"
                     /locus_tag="ACHU9J_000208"
     CDS             11917..12447
                     /gene="emrR"
                     /locus_tag="ACHU9J_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1903868.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12574..13746
                     /gene="emrA"
                     /locus_tag="ACHU9J_000209"
     CDS             12574..13746
                     /gene="emrA"
                     /locus_tag="ACHU9J_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1903869.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13763..15301
                     /gene="emrB"
                     /locus_tag="ACHU9J_000210"
     CDS             13763..15301
                     /gene="emrB"
                     /locus_tag="ACHU9J_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1903870.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15365..15880)
                     /gene="luxS"
                     /locus_tag="ACHU9J_000211"
     CDS             complement(15365..15880)
                     /gene="luxS"
                     /locus_tag="ACHU9J_000211"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1903871.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16030..17586)
                     /gene="gshA"
                     /locus_tag="ACHU9J_000212"
     CDS             complement(16030..17586)
                     /gene="gshA"
                     /locus_tag="ACHU9J_000212"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1903872.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17659..18087)
                     /locus_tag="ACHU9J_000213"
     CDS             complement(17659..18087)
                     /locus_tag="ACHU9J_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1903873.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18084..18650)
                     /gene="yqaB"
                     /locus_tag="ACHU9J_000214"
     CDS             complement(18084..18650)
                     /gene="yqaB"
                     /locus_tag="ACHU9J_000214"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1903874.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSIEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18933..19009)
                     /locus_tag="ACHU9J_000215"
     tRNA            complement(18933..19009)
                     /locus_tag="ACHU9J_000215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18973..18975),aa:Arg,seq:acg)
     gene            complement(19208..19284)
                     /locus_tag="ACHU9J_000216"
     tRNA            complement(19208..19284)
                     /locus_tag="ACHU9J_000216"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19248..19250),aa:Arg,seq:acg)
     gene            complement(19348..19424)
                     /locus_tag="ACHU9J_000217"
     tRNA            complement(19348..19424)
                     /locus_tag="ACHU9J_000217"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19388..19390),aa:Arg,seq:acg)
     gene            complement(19428..19520)
                     /locus_tag="ACHU9J_000218"
     tRNA            complement(19428..19520)
                     /locus_tag="ACHU9J_000218"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19484..19486),aa:Ser,seq:gct)
     gene            complement(19836..20021)
                     /gene="csrA"
                     /locus_tag="ACHU9J_000219"
     CDS             complement(19836..20021)
                     /gene="csrA"
                     /locus_tag="ACHU9J_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1903875.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20256..22886)
                     /gene="alaS"
                     /locus_tag="ACHU9J_000220"
     CDS             complement(20256..22886)
                     /gene="alaS"
                     /locus_tag="ACHU9J_000220"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1903876.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23014..23514)
                     /gene="recX"
                     /locus_tag="ACHU9J_000221"
     CDS             complement(23014..23514)
                     /gene="recX"
                     /locus_tag="ACHU9J_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1903877.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23742..24803)
                     /gene="recA"
                     /locus_tag="ACHU9J_000222"
     CDS             complement(23742..24803)
                     /gene="recA"
                     /locus_tag="ACHU9J_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1903878.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(24883..25380)
                     /gene="pncC"
                     /locus_tag="ACHU9J_000223"
     CDS             complement(24883..25380)
                     /gene="pncC"
                     /locus_tag="ACHU9J_000223"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1903879.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25525..26610)
                     /gene="mltB"
                     /locus_tag="ACHU9J_000224"
     CDS             complement(25525..26610)
                     /gene="mltB"
                     /locus_tag="ACHU9J_000224"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1903880.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            26866..27429
                     /gene="srlA"
                     /locus_tag="ACHU9J_000225"
                     /gene_synonym="gutA"
     CDS             26866..27429
                     /gene="srlA"
                     /locus_tag="ACHU9J_000225"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1903881.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27426..28385
                     /gene="srlE"
                     /locus_tag="ACHU9J_000226"
                     /gene_synonym="gutE"
     CDS             27426..28385
                     /gene="srlE"
                     /locus_tag="ACHU9J_000226"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1903882.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            28396..28767
                     /gene="srlB"
                     /locus_tag="ACHU9J_000227"
                     /gene_synonym="gutB"
     CDS             28396..28767
                     /gene="srlB"
                     /locus_tag="ACHU9J_000227"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1903883.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28771..29539
                     /gene="srlD"
                     /locus_tag="ACHU9J_000228"
                     /pseudo
     CDS             28771..29539
                     /gene="srlD"
                     /locus_tag="ACHU9J_000228"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            29645..30004
                     /gene="gutM"
                     /locus_tag="ACHU9J_000229"
     CDS             29645..30004
                     /gene="gutM"
                     /locus_tag="ACHU9J_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1903884.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30071..30844
                     /gene="srlR"
                     /locus_tag="ACHU9J_000230"
                     /gene_synonym="gutR"
     CDS             30071..30844
                     /gene="srlR"
                     /locus_tag="ACHU9J_000230"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1903885.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            30837..31802
                     /gene="gutQ"
                     /locus_tag="ACHU9J_000231"
     CDS             30837..31802
                     /gene="gutQ"
                     /locus_tag="ACHU9J_000231"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1903886.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31799..33313)
                     /gene="norR"
                     /locus_tag="ACHU9J_000232"
     CDS             complement(31799..33313)
                     /gene="norR"
                     /locus_tag="ACHU9J_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1903887.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            33500..34939
                     /gene="norV"
                     /locus_tag="ACHU9J_000233"
     CDS             33500..34939
                     /gene="norV"
                     /locus_tag="ACHU9J_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1903888.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAKEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            34936..36069
                     /gene="norW"
                     /locus_tag="ACHU9J_000234"
     CDS             34936..36069
                     /gene="norW"
                     /locus_tag="ACHU9J_000234"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1903889.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36197..38449)
                     /gene="hypF"
                     /locus_tag="ACHU9J_000235"
     CDS             complement(36197..38449)
                     /gene="hypF"
                     /locus_tag="ACHU9J_000235"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1903890.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38602..39129)
                     /gene="hydN"
                     /locus_tag="ACHU9J_000236"
     CDS             complement(38602..39129)
                     /gene="hydN"
                     /locus_tag="ACHU9J_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1903891.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39277..40287)
                     /gene="ascG"
                     /locus_tag="ACHU9J_000237"
     CDS             complement(39277..40287)
                     /gene="ascG"
                     /locus_tag="ACHU9J_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1903892.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            40547..42004
                     /gene="ascF"
                     /locus_tag="ACHU9J_000238"
     CDS             40547..42004
                     /gene="ascF"
                     /locus_tag="ACHU9J_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1903893.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWICS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            42013..43437
                     /gene="ascB"
                     /locus_tag="ACHU9J_000239"
     CDS             42013..43437
                     /gene="ascB"
                     /locus_tag="ACHU9J_000239"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1903894.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43551..43970)
                     /locus_tag="ACHU9J_000240"
     CDS             complement(43551..43970)
                     /locus_tag="ACHU9J_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1903895.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(43967..44278)
                     /locus_tag="ACHU9J_000241"
     CDS             complement(43967..44278)
                     /locus_tag="ACHU9J_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR1903896.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(44440..45213)
                     /locus_tag="ACHU9J_000242"
     CDS             complement(44440..45213)
                     /locus_tag="ACHU9J_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1903897.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(45238..45708)
                     /gene="hycI"
                     /locus_tag="ACHU9J_000243"
     CDS             complement(45238..45708)
                     /gene="hycI"
                     /locus_tag="ACHU9J_000243"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1903898.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45701..46111)
                     /gene="hycH"
                     /locus_tag="ACHU9J_000244"
     CDS             complement(45701..46111)
                     /gene="hycH"
                     /locus_tag="ACHU9J_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1903899.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46108..46875)
                     /gene="hycG"
                     /locus_tag="ACHU9J_000245"
     CDS             complement(46108..46875)
                     /gene="hycG"
                     /locus_tag="ACHU9J_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1903900.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(46875..47417)
                     /gene="hycF"
                     /locus_tag="ACHU9J_000246"
     CDS             complement(46875..47417)
                     /gene="hycF"
                     /locus_tag="ACHU9J_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1903901.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47427..49124)
                     /gene="hycE"
                     /locus_tag="ACHU9J_000247"
     CDS             complement(47427..49124)
                     /gene="hycE"
                     /locus_tag="ACHU9J_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1903902.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(49142..50065)
                     /gene="hycD"
                     /locus_tag="ACHU9J_000248"
     CDS             complement(49142..50065)
                     /gene="hycD"
                     /locus_tag="ACHU9J_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1903903.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(50068..51894)
                     /gene="hycC"
                     /locus_tag="ACHU9J_000249"
     CDS             complement(50068..51894)
                     /gene="hycC"
                     /locus_tag="ACHU9J_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1903904.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(51891..52502)
                     /gene="hycB"
                     /locus_tag="ACHU9J_000250"
     CDS             complement(51891..52502)
                     /gene="hycB"
                     /locus_tag="ACHU9J_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1903905.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52627..53088)
                     /gene="hycA"
                     /locus_tag="ACHU9J_000251"
     CDS             complement(52627..53088)
                     /gene="hycA"
                     /locus_tag="ACHU9J_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1903906.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53300..53650
                     /gene="hypA"
                     /locus_tag="ACHU9J_000252"
     CDS             53300..53650
                     /gene="hypA"
                     /locus_tag="ACHU9J_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1903907.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53654..54526
                     /gene="hypB"
                     /locus_tag="ACHU9J_000253"
     CDS             53654..54526
                     /gene="hypB"
                     /locus_tag="ACHU9J_000253"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1903908.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54517..54789
                     /gene="hypC"
                     /locus_tag="ACHU9J_000254"
     CDS             54517..54789
                     /gene="hypC"
                     /locus_tag="ACHU9J_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1903909.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            54789..55910
                     /gene="hypD"
                     /locus_tag="ACHU9J_000255"
     CDS             54789..55910
                     /gene="hypD"
                     /locus_tag="ACHU9J_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1903910.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            55907..56917
                     /gene="hypE"
                     /locus_tag="ACHU9J_000256"
     CDS             55907..56917
                     /gene="hypE"
                     /locus_tag="ACHU9J_000256"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1903911.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            56991..59068
                     /gene="flhA"
                     /locus_tag="ACHU9J_000257"
                     /pseudo
     CDS             56991..59068
                     /gene="flhA"
                     /locus_tag="ACHU9J_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(59105..59458)
                     /locus_tag="ACHU9J_000258"
     CDS             complement(59105..59458)
                     /locus_tag="ACHU9J_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1903912.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(59535..59669)
                     /locus_tag="ACHU9J_000259"
     CDS             complement(59535..59669)
                     /locus_tag="ACHU9J_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1903913.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            59745..62306
                     /gene="mutS"
                     /locus_tag="ACHU9J_000260"
     CDS             59745..62306
                     /gene="mutS"
                     /locus_tag="ACHU9J_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1903914.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62412..62825
                     /locus_tag="ACHU9J_000261"
                     /pseudo
     CDS             62412..62825
                     /locus_tag="ACHU9J_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        14885 a        16946 c        16222 g        14772 t
ORIGIN      
        1 atcggcatgt aaaaatccac aatgtacaaa aaacgagccg ttacggaata ttttatctac
       61 aaaaactgac taaataaaaa tttttcacta attgaatagt catagccagc gatatacgct
      121 atgcgaaaat gcagatggca atgagatcca ctgctttcat ctccattaac atcccattac
      181 gcttttatta aggagcatta gcatgttctc accgcagtca cgcttgcgtc atgcagttgc
      241 agatacgttc gcgatggttg tttactgttc tgtcgtgaac atgtgtattg aagttttcct
      301 ctccggaatg agcttcgaac agtcttttta ttccagattg gtagcgattc cggtgaacat
      361 cttaattgca tggccatacg gtatgtaccg cgatctattt atgcgcgcgg cacgcaaagt
      421 tagcccgtcg ggctggataa aaaatctggc tgatatcctg gcttatgtga cgttccagtc
      481 accggtgtat gtggcgatct tgttagtggt gggcgcagac tggcatcaga ttatggcggc
      541 ggtcagttca aacatcgttg tttcgatgtt gatgggggcg gtttatggct acttcctcga
      601 ttattgccgc cgactgttta aagtcagcca ttaccagcag gtaaaagcct gactcttctt
      661 ttcgcgactg gcatcgccag tcgcgtcttt aactggcttc gccaaataaa ccattcaaat
      721 aacgttcaag cgcaacacgc gaactgaagc catgcgggat accgtaatgc tcgtgtcgct
      781 cgcctgctaa atagagcgga aattgttgat aatgatcgca agttttaata tcggaagcgg
      841 gttcagccag tgtcgaacta cgttctttta atgttggatg aattaccagt gcggtacgtc
      901 ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa ccataagctt
      961 tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg
     1021 gtcgtaaata catatttgat cctcgttatt atcaaccgcg ggcctacctt acctgattgc
     1081 gcattcacaa cgctttcaga aaagtccata aagccgcgat gaacctcagt taagaaaatt
     1141 atggtctaca ctgaaaatta catcgaattc taatggggga tcatatgttt aaccgaccga
     1201 accgcaacga cgttgatgat ggcgtgcagg atatccagaa tgatgtcaat caattagctg
     1261 acagcctcga atctgtattg aagtcctggg gcagcgacgc caaaggggaa gctgaagccg
     1321 cacgcagtaa agctcaggca ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc
     1381 gcgtccagca agccgcacgc gatgccgttg gctgcgcgga ttcttttgtt cgtgaaagac
     1441 cctggtgtag cgtgggtaca gcagctgcgg taggtatttt tatcggcgca ctgttaagca
     1501 tgcgcaaatc gtagtgcaaa aatgataata aatacgcgtc tttaaccccg aagcctgtct
     1561 tcggggtttc tttttgcctg gtgaatcaca aaaatccccc tcccccgtca cgctcatatc
     1621 tagggtaatt tcgatcacta tttgctatat attgtgtggt tgaatctttt ttcaactaca
     1681 tctagtatct ctgtatcaac agagagacaa cccgacgcgt atcatcgcgc cgtatcttca
     1741 ttttaaacgg aaatacgaat catgcgcata actatttaca ctcgtaacga ttgcgttcag
     1801 tgccacgcca ccaaacgggc gatggaaaac cggggctttg attttgaaat gattaatgtc
     1861 gatcgcgttc ctgaagcagc ggaagcgttg cgtgctcagg gctttcgtca gttgccggta
     1921 gtgattgctg gcgatcttag ctggtctggt ttccgtccgg acatgattaa ccgtctgcat
     1981 ccagcgccac acgcggccag tgcatgagcc agctcgtcta cttctccagc agctccgaaa
     2041 acacgcagcg ttttatcgaa cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc
     2101 gggaacggat tcaggtagac gagccttaca tcctgatcgt gccctcttac ggcggcggcg
     2161 gtacggctgg cgcggtgcca cgataggtaa ttcgcttttt aaacgacgag cacaaccggg
     2221 cgttgcttcg cggcgttatt gcttctggta atcgcaactt tggtgaggcg tatggccgcg
     2281 ccggagatgt gattgcccgg aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg
     2341 gtacgcaaag cgatatcgaa aacgttcgta aaggagtaac cgaattttgg caacgacaac
     2401 cgcagaatgc ctgacgcagg aaacgatgga ttaccacgcg ctgaatgcga tgcttaacct
     2461 ctacgatagc gcaggtcgca ttcagttcga taaagaccgc caggccgttg acgcctttat
     2521 ggcgacgcat gtgcgtccga acagtgtgac cttcagtagc cagcagcagc gcctgaactg
     2581 gctggtcaac gaaggttact atgatgaaag cgttcttaat cgctactctc gcgactttgt
     2641 cattacgctg tttgcccacg cacacaccag cggttttcgt ttccagacat tcctcggggc
     2701 atggaagttt tacaccagct atacgttgaa gacattcgac ggtaaacgtt atctggaaga
     2761 ttttgccgat cgagtcacga tggtggcgct gacgctggca caaggcgatg cgacgctggc
     2821 gttgcaactg accgatgaaa tgctgtcagg acgctttcag ccagccacgc cgacattcct
     2881 caactgcggt aagcagcagc gcggcgaact ggtttcctgt tttctgctgc gtattgaaga
     2941 caatatggag tcgattggcc gggcggtaaa ttccgcactg caactgtcga aacgcggcgg
     3001 cggcgtggca tttttgctgt cgaatctgcg agaagcgggc gcgccaatta aacgtattga
     3061 aaatcaatct tctggcgtaa ttccggtgat gaaaatgctg gaagacgcat tttcctatgc
     3121 caaccaactc ggcgcgcgtc agggggcagg tgcagtctat ttacatgctc atcatcccga
     3181 tattctgcgt tttctcgata caaaacggga aaatgccgac gaaaaaatcc gcattaaaac
     3241 actgtcgctg ggcgtggtga tcccggatat cactttccat ctggcaaaag agaatgcgca
     3301 gatggcgctg ttttcgccct atgacgttga gcgagtttat ggcaagccgt ttgccgatat
     3361 cgccatcagc gaacactatg acgaactggt tgccgatgaa cgcatccgca aaaaatacct
     3421 caacgcccgt gatttcttcc agcggctggc agaaatccag tttgagtccg gctatcccta
     3481 catcatgtat gaagacacgg taaaccgtgc taaccctgtc gccgggcgca taaatatgag
     3541 taatctctgc tcagagattt tgcaggtcaa ctgtgcctca gagtatgacg agaatctcga
     3601 ctatgcccgc acaggccatg atatctcctg caatttaggt tcgttgaata ttgcgcacac
     3661 catggattcc cccgattttg cccgcacggt agagactgcc gtgcgcggtt taacggcggt
     3721 gtcagatatg agtcatatcc gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcaca
     3781 cgccatcgga ctggggcaga tgaatttaca cggctatctg gcgcgagaag gcatcgctta
     3841 tggttcgccg gaagcactgg atttcaccaa tctctatttc tatgccatca cctggcacgc
     3901 actgcgtacc tcgatgttgc tggcgcgcga acgcggtgaa accttcgccg ggttcaaaca
     3961 gtcacgttat gccagtggtg aatattttag ccaatatctg caaggtaact ggcagccgaa
     4021 aacggcgaaa gttggcgaac tgtttgcccg cagcggtatt acgttaccta cccgtgagat
     4081 gtgggcgcaa ctgcgcgacg acgtgatgcg ctacggcatt tacaaccaga atcttcaggc
     4141 ggtgccgcca accggttcta tctcttatat caaccatgct acgtcgagta ttcatccgat
     4201 tgtggcgaaa gtagagatac gcaaagaggg caaaacagga cgcgtttact accctgcccc
     4261 gtttatgact aacgaaaatc tggcgctgta tcaggatgct tacgaaatcg gcgcagaaaa
     4321 gatcatcgac acctacgcgg aagcgactcg ccatgtcgat cagggtttgt cgctgacgct
     4381 gtttttcccc gataccgcca ccactcgcga tatcaacaaa gcgcagattt acgcctggcg
     4441 caaaggtatc aaaacgctct attacattcg cctgcgtcag atggcgctgg aaggcactga
     4501 aattgaaggc tgcgtctcct gtgcacttta aggaatatct atgaaactct cacgtatcag
     4561 cgccatcaac tggaacaaga tatctgacga taaagatctg gaggtgtgga atcgcctgac
     4621 cagcaatttc tggctaccag aaaaggtgcc gctgtcgaac gatattcctg cctggcagac
     4681 attaactgtc gtagaacaac aactgacgat gcgcgttttt actggcctga cgctgctcga
     4741 cacgctgcaa aatgttatcg gcgcgccttc gctgatgccc gatgcactca cgcctcatga
     4801 agaagcggta ttatcgaata tcagctttat ggaagcggtt catgcccgct cttacagttc
     4861 gattttctcg acgctgtgcc agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga
     4921 aaacgcaccg ttgcagcgaa aagctcagat tattcagcaa cattatcgcg gtgatgatcc
     4981 gctgaaaaag aaaatcgcca gtgtgtttct tgaatctttt ttgttctatt ccggtttctg
     5041 gctgccgatg tatttttcca gccgcggaaa gctgaccaat accgcagacc tgatccgtct
     5101 gattatccgt gatgaagcag tccacggtta ctacataggc tataaatatc agaaaaacat
     5161 ggaaaagata tctctgggac aacgtgaaga gttgaagagt ttcgccttcg atttgttgct
     5221 ggaactctac gacaacgagc tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc
     5281 tgacgatgtg aaagcgtttc tctgttacaa cgccaataag gctttgatga atctgggcta
     5341 cgaaccttta tttcccgcag aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc
     5401 gccgaatgcc gatgaaaatc acgatttctt ttccggttca ggctcctctt atgtgatggg
     5461 gaaagcggtt gaaacagaag atgaagactg gaatttctga gggtgttatt ttcaaaaata
     5521 tcactacccg cgacagggaa ataattcccg ccaaatagct ttttatcacg caaatatttt
     5581 gtggtgatct acactgatac tctgttgcat tattcgcctg aaaccacaat attcaggcgt
     5641 tttttcgcta tctttgacaa aaaatatcaa cttttctcga tttgctctca gcccttatat
     5701 cacgggaaat tccggcgatt tgctcgcatc aatattcatg ccacatttgc catcaggggt
     5761 tgcctcagat tctcagtatg ttagggtaga aaaaagtaac tatttccatt gggtaatata
     5821 tcgacataaa caaataacag gaatctttct attgcatggc aattaaatta gaaattaaaa
     5881 acctttataa aatatttggt gagcatccac agcgagcatt caaatatatc gaacaaggac
     5941 tttcaaaaga acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc
     6001 tggccattga agaaggcgag atatttgtca tcatgggatt atccggctcg ggtaaatcca
     6061 caatggtacg ccttctcaat cgcctgattg aacccacccg cgggcaagtg ctgattgatg
     6121 gtgtggatat tgccaaaata tccgatgcca aactccgtga ggtgcgcaga aaaaagattg
     6181 cgatggtctt ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt
     6241 tcggtatgga attggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac
     6301 tgcgtcaggt cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga
     6361 tgcgtcaacg tgtgggatta gcccgcgcgt tagcgattaa tccggatatt ttattaatgg
     6421 acgaagcctt ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa
     6481 aattacaggc gaaacatcag cgcaccattg tctttatttc ccacgatctc gatgaagcca
     6541 tgcgtattgg cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac
     6601 cggatgaaat tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg
     6661 atattagtca ggtattcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc
     6721 gtaaaacccc tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgcg
     6781 aatatggcta cgttatcgaa cgcggtaata agtttgtcgg cgcagtctcc atcgattcgc
     6841 ttaaaaccgc gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag
     6901 cagtcgatgc acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg
     6961 cggtgcccgt ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc
     7021 tgcgcgcttt agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc
     7081 acgccagcgg cggacagtgc cgcgcaatcc gcagacgcct ggggtacacc gacgactgca
     7141 ccgactgacg gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat
     7201 tttaatattc tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa
     7261 gggatcgact gggttgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt
     7321 gattatatcc tcaacagttt ccagcaattg ctgctgggta tgccagcgcc agtggcgatt
     7381 atcgttttcg ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg
     7441 gtttcgctga ttgccatcgg cgcaatcggt gcctggtcgc aggcaatggt gactctggcg
     7501 ctggtgttaa ccgccctgct gttctgtatc gtcatcggtt tgccgttggg gatatggctg
     7561 gcgagaagtc cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg
     7621 ccagcgtttg tttatctggt accaatcgtc atgctgtttg gtatcggtaa cgtgccgggc
     7681 gtagtggtga cgatcatctt tgctctgccg ccgattatcc gtctgacgat tctggggatt
     7741 aaccaggttc cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag
     7801 atgctgttca aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag
     7861 acgctgatgc tggctctttc tatggtggtc atcgcctcga tgattgccgt cggcgggctg
     7921 ggtcagatgg tacttcgcgg tatcggtcgt ctggatatgg gccttgccac cgttggcggc
     7981 gtcgggattg tgatcctcgc cattatcctc gaccgcctga cgcaggccgt tgggcgcgac
     8041 tcacgcagtc gcggcaaccg tcgctggtac accactggcc ctgtcggtct gctgacccgc
     8101 ccattcatta agtaactctg cacttgcccg gtaacgccgg gcattatcac cctgccaaaa
     8161 aaaggaataa caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct
     8221 acacaaactt ttgcagccga tctgccgggc aaaggcatta ctgttaatcc agttcagagc
     8281 accatcactg aagaaacctt ccagacgctg ctggtcagtc gtgcactgga gaaattaggt
     8341 tataccgtca ataaacccag cgaagtggat tacaacgttg gctacacctc acttgcttcc
     8401 ggcgatgcaa ccttcaccgc cgtgaactgg acgccgctgc atgacaacat gtacgaagct
     8461 gccggtggcg ataagaaatt ttattgtgaa ggggtattcg ttaacggcgc ggcacagggt
     8521 tacctgatcg ataagaaaac cgccgaccag tacaaaatca ccaacatcgc acaactgaaa
     8581 gatccgaaga tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttaaccggc
     8641 tgtaacccag gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg
     8701 accaacaccg tgacgcataa tcaggggaac tacgcagcga tgatggccga caccatcagt
     8761 cgctacaaag agggcaaacc ggtgttttat tacacctgga cgccgtactg ggtgagtaat
     8821 gagctgaagc cagggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc
     8881 gataaaaacg ccgataccaa actgccgaat ggtgcgaatt atggcttccc ggtcagcacc
     8941 atgcatatcg ttgccaacaa agcctgggcc gagaaaaacc cggcagcagc gaaacttttt
     9001 gccattatgc agttgccagt ggcagatatt aacgcccaga acgccattat gcatgacggc
     9061 aaagcctcag aaggcgatat tcagggccat gttgatggtt ggatcaaagc ccaccagcag
     9121 cagttcgatg gctgggtgaa tgaggcgctg gcagcgcaga agtaattttt attcgggcgg
     9181 ataagtcgtt tttgcctcat ccgccattct gtgcacaatg cctgatgcga cgctggcgcg
     9241 tcttatcatg cctacaaaat acactcattc cgtatggcgg ataaggcgtt ttcgccgcat
     9301 ccgccgttct gtgcacaatg cctgatgcga cgctggcgcg tcttatcatg cctacaaacc
     9361 acatcgcaca tttcaacaag ctatctttcg cccccattca tcaacatccg ctattattga
     9421 tttccagctt aatcatcacc tgatgaacaa aaataatgac taaacctaat catgagctta
     9481 gcccggcgct gatcgtgctg atgtctatcg ccaccggtca ggcggtagcc agtaactatt
     9541 acgcccagcc attgctcgac accatcgcgc gtaacttttc cctttccgcc agttcggcag
     9601 gctttattgt taccgccgcg cagttgggct atgcagcagg tctgctgttt cttgttcccc
     9661 tcggtgatat gtttgaacgc cgccgcctga ttgtctcgat gaccttactg gcggcaggcg
     9721 gcatgttaat cactgccagc agtcagtcgc tggcgatgat gatcctcggt acggcattaa
     9781 ccggtttatt ctcagtcgtg gcacaaattc tggttccgct ggcagcgacg ctggcttcac
     9841 cggataaacg cggcaaagtg gttggcacca ttatgagcgg tctgctgttg gggatcttgc
     9901 tggcacgaac cgttgccggg ttactggcaa acctcggcgg ctggcgcacc gtcttttggg
     9961 ttgcctcggt gttaatggca ctgatggcgc tagcattatg gcgtggtctg ccacaaatga
    10021 aatcagaaac ccacctcaac tacccacagt tgttaggttc cgttttcagc atgtttatca
    10081 gcgataagat cctccgcacc cgcgcattgc tgggctgcct gacctttgcc aacttcagca
    10141 ttctctggac ctcaatggcc tttttgcttg ccgctccacc ttttaactac agcgatggtg
    10201 tcattggtct gtttggactt gcgggagctg ccggagcgtt gggcgctcgt ccggcgggcg
    10261 gttttgccga taagggtaaa tcgcaccaca ccacaacttt cggtctgctg ctgctattac
    10321 tttcatggct ggcgatctgg tttggtcaca cttccgtact ggcgctgatt atcggcatcc
    10381 tggtgctgga tctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    10441 tacaccctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    10501 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    10561 gtctggctgg cgcgacgatt gccctagtta acttactggt ctggtggcga ggttttcatc
    10621 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    10681 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    10741 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    10801 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    10861 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    10921 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    10981 ggagtagttt gtgggttgct gcactgaccg tcatggcaat ggatgttcgc catgtgttgt
    11041 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctgt
    11101 gggcgtttgg cctgacggat gaggtttttg ccgccgcaac agcaaaactg gtacgcaata
    11161 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    11221 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    11281 ttgaagcagc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    11341 tccagcgcaa acaatctctt tgcgttaccg cagcgttagt tggtgccctt gcaggcgtaa
    11401 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    11461 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    11521 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    11581 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    11641 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    11701 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    11761 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    11821 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    11881 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    11941 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    12001 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    12061 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    12121 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    12181 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtga aagcgataac
    12241 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    12301 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    12361 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    12421 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    12481 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    12541 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    12601 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    12661 ctctttataa ttattgccgt agcgataggg atttattgtt ttttggtact gcgtcatttc
    12721 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    12781 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgacgtgctg
    12841 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    12901 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    12961 gaggtgcaga aaatcgccct cgcaaaagca caaagcgact acaaccgccg tgtgccgctg
    13021 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    13081 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    13141 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    13201 tggctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ctcccgccgc
    13261 gcggtacagc ctggggcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    13321 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgtatcggt
    13381 cagccggtca ctatcaccac ggatatttac ggcgatgatg tgaaatacac cggtaaagtg
    13441 gttggtctgg atatgggcac aggtagcgca ttctcactgc ttccggcgca aaatgcgacc
    13501 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    13561 ctggagcaat acccgctgcg cattggtttg tccacactgg tgagcgtcaa taccactaac
    13621 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    13681 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    13741 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    13801 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctggact ccaccattgc
    13861 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc agggaacgtg
    13921 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    13981 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    14041 gtgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    14101 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    14161 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    14221 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    14281 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaactctgc gcggacgtga
    14341 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    14401 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactggtttt catcacagga
    14461 aattatcatc cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    14521 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    14581 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    14641 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    14701 agggattatt ccggtgatcc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    14761 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    14821 tacctttgaa ccaggtatgg attttggcgc gtcggcctgg ccgcagttta tccaggggtt
    14881 tgcggtggcc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    14941 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    15001 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    15061 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    15121 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    15181 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    15241 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggtggcggcg gtgcgcacta
    15301 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    15361 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    15421 tgatgcgtac gtcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    15481 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    15541 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    15601 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    15661 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    15721 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    15781 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    15841 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    15901 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    15961 tttgttatca tcatccctga attcagagat gaaattttgg ccactcacga gtggcctttt
    16021 tcttttctgt caggtgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    16081 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    16141 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    16201 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    16261 attatcgaag caggcaacca gttcatcaca cactttctga tacgcgtcgc cgccgttaat
    16321 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    16381 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    16441 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    16501 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    16561 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    16621 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    16681 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    16741 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    16801 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    16861 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    16921 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    16981 cggagatgca ccaaacagat aaggaatgac ccaaccgaaa cgatagtaat tgcggataac
    17041 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    17101 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    17161 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    17221 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    17281 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    17341 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    17401 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    17461 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    17521 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    17581 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    17641 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    17701 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attaccggtc cccacgagat
    17761 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    17821 tagcgtgact gcaccatagc gtttcagcca gccgatagct ttctcttgcc agcgcgatgt
    17881 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    17941 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    18001 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    18061 cgagaaaagc gataacgctt cactcacagc aagcgaacgt ccacagcgtc catacctgcg
    18121 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    18181 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    18241 tgatcggcag cgacgacggc gtcaaaataa tggcgtaatc ccaggtgcgc cagcaatgcc
    18301 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    18361 ttcaccacat caacaagagg aagcggttcg atgctatcca gcagcatact tcttactgct
    18421 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    18481 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    18541 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc
    18601 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    18661 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    18721 ggagaagaaa attcaggaaa tggataaagg agttatttag aaatgagata tttttgaagg
    18781 aaattttttg gagaagatgg tgcatccggg aggattactc gccttgcggc tcgcccttcg
    18841 ggccgttgct ggcgcaacgt tatccttcct ggtgcctgcg gttaataccg ctatctcttc
    18901 gataccttca ttgctgaagg ttacggagaa tatggtgcat ccgggaggat tcgaacctcc
    18961 gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaaac
    19021 ttattctact gctgatattg ataccgctac taaagccata tcaattaaga gatggtgcat
    19081 ccgggaggat tactcgcctt gcggctcgcc cttcgggccg ttgctaaagc aacgttatca
    19141 tccctggtgc ctgcggttaa ttccgctacc tcttcgatac cttcattgct gaagatttcg
    19201 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    19261 tctatccagc tgagctacgg atgcatcggg aaaatttatt ttactgctga tattgatacc
    19321 gctactaaag ccatatcaag taagagatgg tgcatccggg aggattcgaa cctccgaccg
    19381 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
    19441 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
    19501 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
    19561 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
    19621 ccacactttt atcgtttgca cacttcacgc tcaattagtc tgtaaaaacg tcaaaaagag
    19681 tgttttatct acagaaaaac ggaggtctgg cagatagcag taatgcaaaa aaatggagac
    19741 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
    19801 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
    19861 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
    19921 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
    19981 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
    20041 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
    20101 caatgacaaa cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt
    20161 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
    20221 gtgaagcgag tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag
    20281 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
    20341 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
    20401 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
    20461 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
    20521 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
    20581 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
    20641 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc
    20701 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
    20761 gcatgaacgg tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca
    20821 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
    20881 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca
    20941 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
    21001 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
    21061 atctcttccg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
    21121 accagtgaac ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc
    21181 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
    21241 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
    21301 tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct
    21361 ttatcaccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
    21421 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
    21481 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc
    21541 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc
    21601 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
    21661 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc
    21721 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
    21781 cgggcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
    21841 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
    21901 gtttctttcg cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt
    21961 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
    22021 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact
    22081 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
    22141 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc
    22201 ggttccatcg tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca
    22261 atgtagcggt cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
    22321 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
    22381 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
    22441 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
    22501 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
    22561 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
    22621 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
    22681 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
    22741 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
    22801 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
    22861 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
    22921 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
    22981 tttcttgagt gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
    23041 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
    23101 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcaccagtgc
    23161 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
    23221 ctgattcagt tcctgacgaa tacgtgcagg tccataacct ttgcggctac ggctggcgat
    23281 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
    23341 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
    23401 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
    23461 cgcacgatcc aacaggcgag catatgccgg gcgacgggat gttgattctg tcatggcata
    23521 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca
    23581 gatttaatag tcaatgagtg tgcagccaat tcgttagagg atcgaggtga gagtaatttt
    23641 tcacgccagt gttggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
    23701 cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt
    23761 tctgctacgc cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc
    23821 agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag
    23881 gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct
    23941 gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata
    24001 ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc
    24061 accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata
    24121 cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtggtt
    24181 tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc
    24241 gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc
    24301 gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc
    24361 gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt
    24421 tccagtgcct gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc
    24481 agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta
    24541 ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc
    24601 ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc
    24661 agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc
    24721 atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc
    24781 tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca
    24841 atatgttctg ttgaagcaat tatactgtat actcatacag tatcaagtgt tttgtagaaa
    24901 ttgttgccac aaggtctgca atgcatacgc agtagcctga cgacgcaccg catcacggtc
    24961 gccgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca
    25021 gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga
    25081 cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg
    25141 ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt
    25201 ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc
    25261 ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc
    25321 acgggctttc agcgcctgcc caacctgttc gcttaactgc atcagttcac tgtcagtcat
    25381 cacaacctca gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg
    25441 gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag
    25501 ctcacgcccc cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa
    25561 ctgccagacc gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt
    25621 cggcagaccg taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg
    25681 ttgatggttg cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct
    25741 gtacctggtt ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac
    25801 ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacactac cgatcgcatc
    25861 aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta
    25921 agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag
    25981 cggatcgtcc tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata
    26041 ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt
    26101 ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg
    26161 tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac
    26221 accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacgcagcc atgcgccgtt
    26281 cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac
    26341 cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg
    26401 tttgttcacc attctgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc
    26461 catctgcatc acattgtgct gaggctcaag caggaagccg ccagacggcg ttccggtggt
    26521 cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg
    26581 aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg
    26641 gcaagtatgg taaagcatca cgccccgcac aaggaagcgg tagtcactgc ccgatacgga
    26701 ctttacataa ctcaactcat tcccctcgct atccttttat tcaaactttc aaattaaaat
    26761 atttatcttt cattttgcga tcaaaataac acttttaaat ctttcaatct gattagatta
    26821 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
    26881 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
    26941 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
    27001 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
    27061 tcccgttacc tgctgctacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
    27121 agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc agcctcttat
    27181 agctgccact caatgaatgg cctctttccc catatcaacc ctggcgaact gtttgtttat
    27241 cttggcattg ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagc
    27301 tatctgctgg ttggtctggt caccaacttc tttcgcggct gggtgaccga tctgaccacc
    27361 gccatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca
    27421 acatcatgac gcgtattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc
    27481 tggaagccac gccgggcaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg
    27541 ttgacaaact ggcacaactt actggctggc aggctattga cggatttaaa gaaggtgaac
    27601 ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca
    27661 tctatccgaa acgacgtatt cccaccatta atatccactc gacgggcaag tccagtccgc
    27721 tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg
    27781 tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac accagcaaga
    27841 aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggtatg ggttctgccg
    27901 ttgccgtgtt gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc
    27961 cgtttatggc gttcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact
    28021 ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc
    28081 tcatctgctc cttcccgctg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg
    28141 ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt
    28201 taccggcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt
    28261 cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc
    28321 actttttaac cggcgcaccg actgtactga tcgcctggtt tgtctccggt tttatctatc
    28381 aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt
    28441 gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac
    28501 ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt
    28561 ttgcaattta cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa
    28621 gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa
    28681 tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt
    28741 ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt
    28801 gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg
    28861 gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat
    28921 ggtgaaggaa cagcgtatag ttttggtgct gacgccacca gcgagcaaag cgtcctggcg
    28981 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta cagcgccgga
    29041 atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg tgaatctggt
    29101 gggttatttc ctgtgtgccc gtgaattttc tcgtttgatg atccgcgacg ggattcaggg
    29161 tcgcattatt cagatcaact cgaaatccgg caaagtgggc agcaaacaca actctggcta
    29221 cagcgcagcg aaatttggtg gcgtcgggct gactcaatca ctggcgctgg atctggcgga
    29281 gtacggcatt acggtgcatt cactgatgct cggtaacctg ctgaaatcgc ctatgttcca
    29341 gtcactgtta ccccaatacg cgaccaagct gggtatcaaa ccggatcaag tcgagcagta
    29401 ttacatcgac aaagtaccgc tcaaacgcgg ctgcgattat caggatgtgc tgaatatgct
    29461 gctgctctat gccagtccta aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg
    29521 cggtcaggtg atgttctaat caacagcgga gatccgttaa ggatctccgt gagactaata
    29581 gaatgcctga tgcgctacgc tcatcaggca tacaggactt ccgccactac attaaggaaa
    29641 agttatggta tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc aactggcctt
    29701 aggcggctgg caaatttctc gttttaaccg tgccttcgac acactatgcc agcaagggcg
    29761 ggttggcgtg ggccgttcca gcgggcgctt taaaccacgg gtcgtggtcg ccatcgccct
    29821 ggacgatcag cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg tcttcgcacg
    29881 accgcaaaaa attcccgcaa ttaccggtat gcatgcgggt gatttacagc ccgatgtgat
    29941 ctttccccat gatccactat cacagaatgc tctatcattg gcgcttaaac tgaaacgtgg
    30001 ataatttcgt tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc taaatcaggt
    30061 aatcacgccc atgaaacctc gtcagcgtca ggccgccatt ctggagtatc tgcaaaagca
    30121 gggtaaatgc tcggttgaag aattggcgca atactttgac accacaggca caaccattcg
    30181 caaagatctg gtcattctgg aacatgcagg aaccgtcatt cgtacttatg gcggagtggt
    30241 gttgaataaa gaggaatccg atccgcctat cgatcataaa acactcatca acacccacaa
    30301 gaaagagctg atagcagaag ctgccgttag ttttattcat gatggcgatt cgatcattct
    30361 tgatgctggc agtaccgttt tgcagatggt tcccctgctc tcgcgcttta ataacatcac
    30421 ggtgatgacc aacagtctgc atatcgtcaa tgcgctatct gaactggata acgaacaaac
    30481 tatcctgatg ccaggcggaa cgtttcgtaa aaaatcggcc tcatttcacg gacagctggc
    30541 agagaatgcc ttcgaacatt tcagtttcga taaattgttt atgggcaccg acggcatcga
    30601 tctcaatgcg ggcgtaacca cctttaacga ggtttatacc gtcagtaagg caatgtgcaa
    30661 tgccgcgcgc gaagtgattt tgatggcgga ctcatcaaag tttggccgta aaagccccaa
    30721 cgtagtttgc agtcttgaaa gcgtcgataa gctgattacc gacgcaggta tcgatccggc
    30781 gtttcgtcag gcgctggaag agaaagggat cgatgtgatc ataaccggag agagcaatga
    30841 gtgaagcact actgaacgcg ggacgtcaga cgttaatgct ggagttgcag gaagcaagcc
    30901 gtttaccgga acgtctgggc gatgattttg ttcgcgccgc caatatcatc ctgcactgcg
    30961 aaggcaaagt ggtggtttcg ggaattggca aatcgggcca cattggtaag aaaatcgccg
    31021 caacacttgc cagtaccggc acatcggctt tttttgtcca tccggcagaa gcactgcacg
    31081 gcgatctggg gatgattgaa agccgcgatg tgatgctgtt tatctcttac tccggtggcg
    31141 cgaaggaact ggatctgatt attccgcgtc tggaagataa atctatcgcg ctgctggcga
    31201 tgaccggcaa accgacgtca ccgctgggcc tggcggcaaa agcggtgctg gatatctccg
    31261 tagaacgcga agcctgcccg atgcaccttg cgccgacctc cagcaccgtc aataccctga
    31321 tgatgggtga cgcgctggcg atggcggtca tgcaggcgcg cggatttaat gaagaagatt
    31381 ttgcccgctc ccacccagcc ggggcactgg gcgctcgctt gctgaataaa gtgcatcatc
    31441 tgatgcgccg tgacgatgcc atcccacagg tggcgttaac cgccagcgtg atggatgcga
    31501 tgctggaact cagccgcacc ggtctggggc tggtggcggt atgtgacgct caacaacagg
    31561 tacaaggcgt ctttaccgac ggcgatttac gtcgctggct ggtaggcggc ggcgcactca
    31621 ccacgccagt taatgaagcg atgacgacag gcggcaccac gttacaggcg caaagtcgcg
    31681 ccatcgacgc caaagagatc ctgatgaagc gcaaaatcac tgccgcaccg gtggtggatg
    31741 aaaacggcaa actcaccggc gcaattaacc tgcaggattt ctatcaggcc gggattattt
    31801 aatccttcat tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtcg gtttccagca
    31861 tccgcgcgca ggcagcccag ttatgatgat tttgtgccag tgcctggcga atagtttcac
    31921 gctggaacgc ttctgtcgct tcacgcaggt tttgcttaac aacgggcacc gccgccgctt
    31981 ctggcggcgg caacgtcacc tcaggaaaag caaaatgttg cgcctcaaga atcacttcat
    32041 cgccgttgcg ggtggctctc gccagcacta ccgcccgatg aatagcatgt tccagttcgc
    32101 gcacgttccc cggaaaacga tagtgttgca gtaaatttcg cgctccggca cttaatacca
    32161 cgcgggagag ccccagccgc aaacgacact gctcgcagaa ataccccgcc agcagaatga
    32221 catcatcgcc ccgctcacgc agcggcggca ccgaaagtgg aaacacgctc aggcgatgaa
    32281 acaagtcagc gcgaaatcgc cctgccatca cctcttcgcg taagtcgcgg ttagtcgccg
    32341 ccagcacgcg cacatcgacc cgcaaactac ggtcatcgcc aacgcgctga atatcgccat
    32401 actgcaacac cctcagcagc ttggcctgca atgccaacga caactcgccg atctcatcca
    32461 gaaacagagt gccgttatcc gccatttcaa acttcccgct gcggttactg atagcgccag
    32521 taaacgctcc tttcacatgc ccgaacaact cactttccgc cacactttcc ggcagtgcag
    32581 cacagttgag atagaccagc ggattcaccg cccgtggcga ggcttcatga atcgctttcg
    32641 ccaccagctc cttaccggtt cccgtctcac cgctgatcag gacgttgaga tcggacgccg
    32701 ccacaatctc aatctctttt ttcagttgcg tcatgccagg ggacaagcca atcatctgcg
    32761 tctgtttcac cgcttcaaac ggcgtggcat cgcctggcat catattctgg ctttccagtt
    32821 gttcaatcag caacgcattg cttaacgctc ccgccgccag cgcagcaatc agccgtagct
    32881 cttcgtcgct gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc agtgcgccga
    32941 tcaggttttg cccggcaaac aatggcagac caacgcaggc gtgaaccttc agactctcct
    33001 gcccaggaat caaaccgtca tagggatcgg gcaattcgct gtctgcggga aagcgcacca
    33061 catccccggc gcgggcaatc gcttccagcc gtggatgccc ttccagcgca aagcgtctac
    33121 cgagtacatc ctttgccaga ccgtcgatgg caagcggaat aaactgccgc gaatcgtaac
    33181 gtagcaacgc agacgcatcg cactccagca cctgacgtag cgtggtgatc aggcgctgaa
    33241 aacgatcctg atgaccaatc ccacgctgca attcgatggc gatattcgcc agcacatcaa
    33301 cggaaaaact catctttgcc tcactgtcaa tttgactata gatattgtca tatcgaccat
    33361 ttgattgata gtcattttga ctactcatta atgagcataa ttttatttat agagtaaaaa
    33421 caatcagata aaaaactggc acgtaatctg caattagcaa gacatctttt tagaacacgc
    33481 tgaataaatt gaggttgcta tgtctattgt ggtgaaaaat aacattcatt gggttggtca
    33541 acgtgactgg gaagtgcgtg attttcacgg tacggaatat aaaacgctac gcggcagcag
    33601 ttacaacagc tacctcatcc gcgaagaaaa aaacgtgctg atcgacaccg tcgaccataa
    33661 attcagccgc gaatttgtgc agaacctgcg taatgaaatc gatctggcgg atatcgatta
    33721 catcgtgatt aaccatgcaa aagaggacca cgctggggcg ctgaccgaac tgatggcgca
    33781 aattcccgat acgccgattt actgtactgc caacgctatc gactcgataa atggtcatca
    33841 tcatcatccg gagtggaatt ttaatgtggt gaaaactggt gacacgctgg atatcggcaa
    33901 cggcaaacag ctcatttttg tcgaaacgcc aatgctgcac tggccggaca gcatgatgac
    33961 ttacctgaca ggcgacgcgg tgctgttcag taacgatgct ttcggtcagc actactgcga
    34021 cgagcatctg ttcaacgatg aagtggatca gacggagctt ttcgagcagt gccagcgtta
    34081 ctacgccaat atcctgacgc cgttcagccg cctggtaaca ccgaaaatta ccgagatcct
    34141 gggctttaac ttaccagtcg atatgatagc gacctctcac ggcgtggtat ggcgcgataa
    34201 cccgacgcaa attgtcgagc tgtacctgaa atgggcggct gattatcagg aagacagaat
    34261 caccattttc tacgacacca tgtcgaataa cacccgcatg atggctgacg ctatcgccca
    34321 ggggattgcg gaaaccgacc cacgcgtggc ggtgaaaatt ttcaacgtcg cccgaagcga
    34381 taaaaacgaa atcctgacca atgtcttccg ctcaaaaggc gtgctggtcg gcacttcgac
    34441 gatgaataac gtgatgatgc cgaaaatcgc cggtctggtg gaggagatga ccggattacg
    34501 cttccgtaac aaacgcgcca gtgctttcgg ctctcacggc tggagcggcg gtgcggtgga
    34561 tcgtctttcc acgcgcctgc aggatgcggg tttcgaaatg tcgcttagcc tgaaagccaa
    34621 atggcgacca gaccaggacg ctctggagtt atgccgtgaa cacggtcgcg aaatcgcccg
    34681 tcagtgggcg ctcgcgccgc tgccgcagag cacggtgaat acggtggtta aagaagaaac
    34741 ctctgccacc acgaagtccg acctcggccc acggatgcag tgcagcgtct gccagtggat
    34801 ttacgatccg gcaaaaggcg agccaatgca ggacgttgcg ccaggaacgc cgtggagtga
    34861 agttccggat aacttcctct gcccggaatg ctctctcggc aaagacgtct ttgacgaact
    34921 ggcatcggag gcaaaatgag caacggcatt gtgatcattg gttcgggctt cgccgcccgc
    34981 caactggtga aaaatattcg caaacaggac gccagtattc cattaaccct gatcgccgcc
    35041 gacagcatgg atgagtacaa caaaccggac ctcagccatg ttatcagcca ggggcaacgt
    35101 gccgacgacc ttacccgcca gacggcgggt gaatttgccg ggcagtttaa tctgcacctg
    35161 tttccgcaca cctgggtgac ggatatcgat gccgaagccc atgtggtgaa aagccagaat
    35221 aatcagtggc aatacgacaa gctggtgctg gcaactggtg ccagtgcctt tgtcccgcca
    35281 gtgcctgggc gtgagttaat gctgacgtta aatagtcagc aagagtatcg cgcctgtgaa
    35341 acgcaactgc gggatgcccg acgcgtgttg attgtcggcg gtggtttgat tggtagcgaa
    35401 ctggcgatgg atttttgtcg tgcaggcaaa gcggtcacgc taatcgacaa cgctgccagt
    35461 attctggcgt cattaatgcc accagaagta agcagccgct tgcagcatcg gttgacggag
    35521 atgggcgttc atctgctgtt gaaatctcag ttgcaggggc tggaaaaaac ggattctggc
    35581 attctggcaa cgctggacca ccagcgtagt atcgaagtgg atgcggtaat tgccgccacc
    35641 ggactgcgcc cggaaacagc cctggcacga cgcgccgggc tgacgattaa tcgcggcgtt
    35701 tgcgtcgata gttatctgca aaccagtaat gacgatattt atgcgctggg cgattgcgcg
    35761 gaaatcaacg gtcaggtact gccattcctg cagccgattc aacttagcgc gatggtgctg
    35821 gcaaaaaatc ttctcggcaa taacacgccg ctgaaactac cggcgatgct ggtgaaaatc
    35881 aaaacgccgg aattaccgct gcatctggca ggcgaaaccc agcgtcagga tttacgctgg
    35941 caaattaata ccgaacgcca gggaatggtg gctcgcggtg ttgacgatgc tgaccagctt
    36001 cgcgcctttg tggtcagtga ggatcggatg aaagaggcat ttggattgtt gaaaacattg
    36061 ccgatgtagg tgggctactg tgcctaaaat gtcggatgcg acgctggcgc gtcttatccg
    36121 acctacgggg acgcatgtgt aggccggata aggcgtttac gccgcatccg gcaatggtgt
    36181 ccaaatgcaa cacgttttat ccgttctgga cttcacccgc taaccaacgc gccgcagcaa
    36241 taaccccctg ccccagagac aaaccgccat cacccgccgg taaactctgt ggaaagagca
    36301 atgtgaaatc agcgagataa tgcgccagac gtgcacgcag caaacggtta tgaataaccc
    36361 cgccgctaaa taccagcgta gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg
    36421 caaaaccctg cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc ggtgcctgcc
    36481 agttcagcca ctgctgccag aaagcggcga gatccagttg attgtccacc agcggcatcg
    36541 tcaccggatg cgtcactccg tggcatgagg ctgcgagcgc ctccagagca caagccgctt
    36601 caccttcata acttaacgtg gctggcgcac agcccagtgc cgccgccact gcatcgaaca
    36661 accgcccaca cgatgacgcc agcggcgcgt taattccacg ctcaatggcc cgcgccagca
    36721 cgctccagtt ttgctgttgc acacttgctg tttcagggta attctgccac tccggcacaa
    36781 agcgcaggca ctgcgccagc aggtttcgcc acggctgctt cgctgccaaa tcgccacccg
    36841 gaagcgccac tgcaggcaag ccgcccaggt gctcacattc gcgatagttc acccgcaggc
    36901 actcgccgcc ccacaaagcg ccgttctccc ccataccgat accgtcgagc gtcaaagcaa
    36961 tgacatcacc gccatccaac ggccactgat gctctgccag acacgccgct gcatgggcat
    37021 gatgatgcag taccgtttgc gtcggcagat tcatttcacg cgcccactgg ctggagacat
    37081 agcccggatg cgcgtcatgc acaacgtatt gcggggtaaa atcgtagatg ttttgcatca
    37141 ggcgtaacgc ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg cccagatgct
    37201 gactcaacac cgcttgttca ccgcgcacca ggcagaaggt atttttcaga tccgcgccga
    37261 gacacagcac aggcggaaca tttttaaagc ccggaggcaa agccagcgca tccggcacat
    37321 acccccgcga acggcgcagc atttcgccgc tttcgcgcac caccgaatca tccatccgct
    37381 gcacgatgtc gcggttatgt atcaagaatc cgtcggcaat gccctgcaaa tccgccagcg
    37441 cctgttcgtt gctgatagct ggtggtttac cgctcaggtt gccggaggtc atcaccagcg
    37501 ggcattgcag ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac attaccccga
    37561 cttcgttaag gtcaggggcg atatcatcac aaagctcagg aacgtatttt ttatccacca
    37621 gcacaatcgg cgcggcgggc gtggtaagca actggcgcgc agcgtctggt aaaccgtcag
    37681 ccactggcaa catgaccgcc agcggtttcg ccgggcgatg tttgcgcgcc cgaagtgtcg
    37741 ccaccgcgtt actgttacgt gcatcgcagg caagatgaaa tccgccaatc cctttgatgg
    37801 cgacaatgtt gcccattttt aactgtgcga tagccgcctg taatgccgcc tcttgttctg
    37861 catgttcacc atgacttacc cattcaagat gtgggccaca ctccgggcag gccaccggct
    37921 gggcgtggaa gcgacgatcg agcgggtcac ggtactcttt gtcacaagcc ggacacaacg
    37981 gaaacgccgc catcacggta aacgggcggt cgtaaggcat ggcgcgaata atggtgaaac
    38041 gcgggccgca gtgggtacag ttgataaacg gataacgata acgccgttcg cctggggtat
    38101 tcatttcggc aaggcaagca gggcaagtag cggcatccgg gacaatttgc gtattcatgg
    38161 cacctcccgc gctctggcgg atggtgaact cagtgggcag ttgtgaccag atgtacggct
    38221 cacgctcgac gctatcaata cgcgccagcg gcgggcagtg ctgatgcaat tgaacaagta
    38281 acgtttccgg gtcttcccgc agccggactt ctacgccatc gccgtcatta cagacatcgc
    38341 cgtgaagatt taattgctgt gccagctgcc agacaaacgg acgaaaaccg acgccctgca
    38401 ctttgccacg aatacgcagt tggacaccgc aagatgtgtt ttttgccatt gagttattcc
    38461 cgccatcatg aattgcgtaa cccgtcctgc cggacacgac tgcgtcgtat ccggcagtct
    38521 caggtcggcg ataccgccag ctccgtattc tacgaatatt tccgggaatt cctttgatgc
    38581 cagaacagtt ctgtaagatt tttagaacat cagcgccgta cggcggcgtt tttctgcact
    38641 cagttgttca agtttattac gatcgacaca aatcagcgca tgagtcgggc aagccgccat
    38701 acacgccggg ccgtcttcac gatggttgca caggtcacat ttattggctt cggctttgtc
    38761 agcccgtaca ttcagccccg cgccgctgtt gcgaatcacc ggacgtacca ccacttccat
    38821 cgcgccatac gggcaagcca caacgcaggt tttgcaacca atgcaacgtt cctgcatcac
    38881 atgaacaaac cctttatcac ggctgatagc accattcggg cagacgttag cgcacggtgc
    38941 atcttcacac tgacggcaaa ctgtcgccgt ggaaatgttc acacctttaa tgacatggat
    39001 acgcggtaaa aatgtttccg gggtcagcga tgcacagtcc tgattttcct gatgagaaac
    39061 cacgcacgcc acttcacagg tacggcaacc aatacattta ctcgcgtcag caatgatgaa
    39121 acggttcatc aaattctcca gcaatgacag ttaatgcgcc gatacattca caaatcatgc
    39181 cagttttaat tcactgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc
    39241 atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaagag caatgagtga
    39301 gtcgcggcgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag
    39361 cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa tttttacgct
    39421 ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga taactgacac
    39481 ctgctctggc accgctacgc cgcgctcgtg caacgctttc atcgcaccta tcgccatatc
    39541 gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc
    39601 ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat
    39661 accatgctgc gccagcgcgt ctttataccc agcaagacgt tcaatgctgg tgggggaatc
    39721 catcgagccg gtaaggaaag caatctcctg atgtccggcg tttatcaact ctgccacggc
    39781 attaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg
    39841 gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga tctcatccac
    39901 gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat
    39961 cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact gccgcccttt
    40021 ctcttccgcc attcgcgcgg catgaaagag taattcacta aaataaatgc cgtggtaaag
    40081 cgtgttggtc actaccaacc ccagcgtctg agtactcttc gccgacagat tgcgcgccag
    40141 caagtttggg cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg
    40201 gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg
    40261 cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt
    40321 gtactgcacc gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg
    40381 gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttagtga caactattca
    40441 tgattttgtg aaaccggttt cctaattccg tttcagcatc ggcatttttc cgttacgtcg
    40501 actgataaca actacatcta ccctactgat aacaggataa aatccgatgg ccaaaaatta
    40561 tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca tctcggcggt
    40621 cacgcactgt atgacgcgtt tgcgcgttgt tatcaaagat gaccagctta tcgacagccc
    40681 gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt
    40741 gattatcggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga
    40801 tatgcagccc gcacagcccg tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg
    40861 gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc
    40921 gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc
    40981 gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctgatggt
    41041 cgcggcatct gccgccatca aatttaaaac caatatgtcg ctggcgattg ccatcgcggg
    41101 tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg gtgaacatgt
    41161 cgaatttgcc ctgattccgg tcaccgcggt gaaatacacc tacacagtga tcccggcgct
    41221 ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta caccggcggt
    41281 gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaatcct
    41341 gctgattggc ccgattggta tctggatctg tagcgccatt tcggcgctgg tttacaccat
    41401 tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc ctctgctggt
    41461 aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg ccgaaaccgg
    41521 caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc
    41581 actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc
    41641 ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg cgatccgcct
    41701 gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg ttgccggtat
    41761 ggcggggctt gccagccact caatggcagc gccagggtta tttaccagcg tgcaattctt
    41821 cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt
    41881 gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg aggaagcggc
    41941 tgccgaggcg cgaaagcatc agagcgtaca accgaccgtc gccaaagaag taagtcttaa
    42001 ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc
    42061 aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaagggc tgaccactgt cgatatgatc
    42121 ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc
    42181 gatgacgagt tttatcccag ccatgaggcg acggattttt atcatcgtta taaagaagat
    42241 atcgccctga tggcagagat gggattcaag gttttccgta cctcaattgc ctggagccgt
    42301 ctctttccgc agggcgatga aatcacgccc aatcagcagg gcattgcttt ttatcgttct
    42361 gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat
    42421 gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt
    42481 ttcagccgct acgcccggac ctgctttgaa gcatttgatg gtctggtgaa atactggctt
    42541 accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg tctggtgttt
    42601 gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc
    42661 agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg
    42721 ctggcgggcg gtaacttcta cccttacagt tgcaagccgg aagatgtctg ggcggcgctg
    42781 gagaaagacc gggaaaacct gttttttatc gatgtgcagg cgcggggcac gtatccggct
    42841 tactctgccc gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc gggcgatgat
    42901 gaaatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc gcgctgcgcc
    42961 tcggcggaga tgaacgccaa caatagcagt gcggcgaacg tagtgaaatc gctgcgtaac
    43021 ccgtatctgc aagtgagcga ctggggctgg ggtattgatc cactcggtct gcgtatcacc
    43081 atgaacatga tgtacgaccg ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc
    43141 gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat cagctattta
    43201 cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac
    43261 accacctggg gctgtattga tttagtctcc gcctctacgg gtgaaatgag caaacgctac
    43321 ggttttgtct acgtagaccg tgacgacgca ggcaacggca cgctgacgcg cacacgtaag
    43381 aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gggaagattt agagtaggta
    43441 acagtgccgg atgcggcgtg aacgccttat ccggcctaca tgtcgcacga accaggaagg
    43501 cccgataagc gcagcgcatc gggcaatctg gggtttggca tcagtcaaaa tcaaagaaac
    43561 cattccggct taacgccaaa tcgggctgaa agtgccttaa tgtgggatat cgtcagggat
    43621 cgttgtccag acaaaatctg actgaccagg gatttagagc cgatttcgtt cttcaaatcc
    43681 gcataggtca gattatgttg atcaatcagc gtgcgaagta acgccacgcc aacaggcatt
    43741 gccgcaacgg ctttgtcgaa ctctgcaaac tcttggttgt tgttttcata ttctgcaatg
    43801 cggctcgcca gaaagtcgat aagtggattg gtgtcatctt cttcaatcaa atattcgacc
    43861 agttccagtg cttcacgata atcatcttct gagtcactac ctcccagaaa ggggactgca
    43921 ttgaccagtt ctcttgttgc tttaacagcg cgtgcagcat tagcagtcat tctttgttct
    43981 ctctgtagta acgtgtcagt ttgtcatact cggcgtgatt agttatgtgc ttcacaaaga
    44041 accgcttatt aacaaagttt atgtaggcaa tcacccttag gttgttccca cctacatcca
    44101 gaacgtacca tttgttgcga tatttaaaat tgtcgaggtt gggaaacgcc gttcgcatct
    44161 cttctggcgt agaaaaatcc gtttccttaa taacccgata caatgcctga agagccagag
    44221 cgtcgttagg atatttacgc gcagactctt caaatggcgc tttagaaatg atgtgcattt
    44281 ttaagtttta acttccttat agtaagtaaa cagccacatc tgcgagttct atccttatgt
    44341 gttcggaaac tatagctcat caaatccaag ttcacaatat gtaaacactt gagcgcgata
    44401 taattacccc caaaatcctc atagccactc tgcaaaaagt cagttctggt tcaccccatc
    44461 aagaacatcc ccctttatca agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc
    44521 gcgcccggcg atgggtttga ggacgagttt acggttgggg gtataagtaa aacgcgtgag
    44581 cgcgggatag cttaacttgc caaaccagcc gtgaacacgc tgccatttgg cgtcttttac
    44641 ctgatacaga ctggttatcc agtaaccgga atcaaactgc atatccagca attgcgtgtg
    44701 accattgcct tgcaaatcca gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc
    44761 cctgggttca aagacacagg gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc
    44821 ataaggagcc agaccattac cggtacttgg taaccagata accagatcct gaatgccgtt
    44881 acgatcaaga tcggcgcgat agagttgccc tttgcccgct atgtttgcca gcatacccac
    44941 aggcacagac caggatttac cggcgcgatc tttaccgttc aattgccatt cactatcatc
    45001 atttggcatc ttttcaagcg tcacgccagc aacgcccact tttctaaaat gccaggtttg
    45061 ctcggcatca taaggcatgg tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct
    45121 gtcactcatt ggcagatatt cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa
    45181 taaaaaagcg cacatccctg ttctgatatt catttcgctg aattccctga tggagaatta
    45241 ttcttcttcc accgctaact gcgcgaagcc gccattccct tcccagcctt ccagtcgttg
    45301 ataaacggtt tctaccgcat ctttaatcgg ctgagtcatc gggtagtaaa agccgacgat
    45361 atccggctgg atgccgagga aaatcacttc gccaatgtct tctttcaact ggtcgataag
    45421 atagttcagc ggcatgttat gggtagtcat cataaacatc tcggcgatat catccgggtc
    45481 gatgatgcgg atttcgccgg ggtttagccc catatcagta gcgtcaacaa tcagtaaccg
    45541 cgtagggcgc agttcacgga tggcgacgat gtcgttttcc ggtgcactac cgccatcaat
    45601 caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg ggccagcgcc
    45661 atcatcgccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc agtctcctca
    45721 ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac agcattgtac
    45781 tccattcctt ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg ttggccagca
    45841 tcggcacatg gttaatgtcg atgacgatct caccatattt cggcacgcct tccattttgc
    45901 ggcgggcttc actgcccgcc tccagagtgg caatccatgc cagatattca tcccacgggc
    45961 aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc gccaggctgt
    46021 aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata aatttacggc
    46081 tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcaa caacatgatt
    46141 cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc agcgtgcaac
    46201 ctgttcttcg ccctgcccta actgtgtcag gaagtcatcg gcaatctgac gaccgtaacg
    46261 ataacccgcc aggcggcgtg cttcgcgatc cactttcaca cgcagcggct gcaccatatc
    46321 gccatgcagg atttccgcct gttgttcatc ctgctcaccc gggccacggg cgtgaatttt
    46381 ctgctccagc aggccgagcg ccattgcaaa gccgtacagc gtggcggcag gcgttggcgg
    46441 gcaaccagga atgtagacat ctaccgggac gattttatcc gtaccgcccc acacgcagta
    46501 gagatcgtgg aaaataccac cgctgttacc gcaggcaccg taggagatac aaattttcgg
    46561 gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg tgaccgcgcc
    46621 ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc cgaagcgttc
    46681 tgcatcaaac agcggcgaaa gcgtggcgaa aatttcgatt tcgcagccgt tgcagccgcc
    46741 gcagtccacg cgataaacgt aggcagaacg tttgattttt ttcagtaacg acgccttcat
    46801 gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt cacgggggcc
    46861 taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat acggtcggac
    46921 ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc gcggtggttt
    46981 tccgcgcggc tgtcgccgtt gtgcttaagc agcgcaatgg cgtagtcgat ctctttctgg
    47041 acggcgaaag gacgattgca gacgcggcag ttgcacagcg cgaagcggga ctgttgcagg
    47101 aagtcttctt tcttccacac tgccagttcg tactcttgcg acagtttgat cgccgccgtc
    47161 gggcaaactt cttcgcagcg tccacagaag atgcagcgcc cgagattaaa ctcccacgcc
    47221 agctcaccgg tggcgaggtc agtttcaacc gttaaggcgt ttgacgggca ggcattgacg
    47281 caggccgcac agccgatgca ctgctgcggg ttctgctccg gcttaccacg gaagttttta
    47341 tcaaccgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt gatgactttt
    47401 ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg ctcaatgctg
    47461 taacgctcga gttctttgta cggcaccact ttgctctttt tcttacgcac atcgaccacg
    47521 gtcatgcggt cggtacagga gtagcaaggg tccaagctac cgataatcag cggcgcatcg
    47581 gaaacggtgt taccgcgcag catgtagcgc agggtcggcc agttcgcgta ggtcgcggca
    47641 cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg gatatcgtcg
    47701 ccgcgcggcg cttcggcaaa gcccagcgcg aaacggtgcg gaatgtaggt aaagccttcc
    47761 accatcagcg gaccgcccgg caggttatcc agaccgtagt cgatcatgtt cagcgcggta
    47821 taaacttcgt tgatacgcac tttcagacgg gaaataacgt cgcagccctg ctcgctgtgg
    47881 acttccattg gcagcaggcc ataaccgaca aacgggtgat cggcgcgagt atcgcgggcg
    47941 tgaccgctgg cgcgaaccat cgggccaacg ttactgaaat cgcgggccat ttccgggtcc
    48001 agacgaccaa tgccgacggt gcgctgttcc atgttcggcg tactcagcag cacatccacc
    48061 agctcctgca cttcacgacg catctgttgc gccagctggc gggtctggat catgtcatct
    48121 ttcaacagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt acgcgcaccg
    48181 gtgaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg cataaagccg
    48241 gagtcaaagc cggtaaagtg gcaggcgaga ccgaggttga gcagatgcga gtgcaggcgt
    48301 tccacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg aatacccatc
    48361 gcgttttcta ccgacgtggt gtaggcggtg ctgtgggcaa agccgcagat cccgcacaca
    48421 cggtcagaga ggaaggtcac ttcgttgtaa cccatacggg tttccgccag tttttccatg
    48481 ccgcgatgaa cgtagaacag acggtagtcg gcgtcgataa tgttttcgcc atcgacgaac
    48541 agacggaagt ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg cacgacgttg
    48601 tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt ggtcggtgcc
    48661 ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc cggcagcacc
    48721 agacggcgtt catccggcag accaaccgga atcaaaccgt acatatcgcg cacttcgcgc
    48781 tcgccccaca ccgccgccgg aacgcgcggc gtcacggacg gatactccgg tttgttggca
    48841 tcaacttcga cgcgaaccgt tacccagcac ttagtcccct tctccatcga cagcacgtaa
    48901 taaacggcgt aatgaccatt cagtttgcgt tcgtcgttac caaaaagcac cgacaaccag
    48961 cccccctgct tgtagtagag aaactccacc acttccggca gatagttcac ctttacggtg
    49021 acagtcagct gatctttggt ctgccaggcg tggtccagca cgacgcccgg aaatgcctca
    49081 ttcagcgcgg cgagataatg ttgacctaat ttttcttcag acatgctcaa actctcttta
    49141 atcacgccgc cagcaaggag acgaacgcta aaaatgcaaa gccaaaccca gtccaggtaa
    49201 tgcgcggagt aatatcaaga cgcagacggg ccatgctgtt ttcgaacagc gcgataacca
    49261 ggacgccgac gaccagtttg acgatggcaa tcaccagcgc cagcagcagc ccaccggcgg
    49321 tgaaggtttc catctgtccc cacgggataa atacgccgac gaacatctgc agcaccacca
    49381 gctgtttcag gctgataccc catttcatga cgccaaagcc gctgccgctg tattcagaga
    49441 gcgggccttc ctgcagctcc tgctcggctt ccgccaggtc gaacggcagt ttgcccattt
    49501 cgataaaggt ggcgaacgca caggcacaaa gcgccagtac cagcgggatg ctctggctca
    49561 gcggccagtg ataaacggtg tcggtgatgt tgctgatgtt ggtggaaccg gcaacctgtg
    49621 cggcaaccca cagaccaagc agcagcatcg gttcgaccag cacgccaagc atcgcttcac
    49681 ggctcgcgcc gatagcggta aacgggctac cggtatccag accagaaatg gcaaagaaga
    49741 aacgcgcgat ggcaaagaga tacagtaagg tgattaaatc acccagttgc ggcagcggag
    49801 aaccgacggt caccaccggc agcgcagtag cgatagtcag catgacgccc accatcacat
    49861 acggcgtcag gcggaacacc cagccggagg catccgggcc aacgctctgg cggcccagca
    49921 gtttgaaaat gtcacgatac tcctgcaaca cacccggccc acgacggtta tgcagacggg
    49981 cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac accagcgcct
    50041 gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa tcaccaccag
    50101 taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt cgcactgcca
    50161 gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg gcgcaaacgc
    50221 ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt catggtcgta
    50281 gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt tgcaaatcgc
    50341 cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca ttggttgaga
    50401 aacggtggtg ttagcaggct ccagaggcag aggtacagca gcagagagca tcggcagcag
    50461 ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta cgcttacgct
    50521 catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg cgccgaggaa
    50581 cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa ttgccagacc
    50641 cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccgctattgc tcagtttgaa
    50701 gaaggattga tagataaccc attccccggc aaacccgttc agtggcggca gcgcagccat
    50761 tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc caataccacc
    50821 gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc cgaggaacag
    50881 tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca ggccaagagc
    50941 aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc ccagcaggat
    51001 aatgccgata ttttccaggg tgtggtaagc cagcaggcgc tggatgttgt gttccatcaa
    51061 cgcatacaga ccaccgacga acgcggtgat catgccgagc accagcagcg cgatccccca
    51121 ccacagcggt gcattaccgc ccagcagtga cagggttaac atgcccagca ggccaatttt
    51181 catgactacc gtagaaaaca acgcggcagc tggcgcagag gcgttagcat gtgcctgcgg
    51241 cacccagccg tgcagcggaa taatcccggc cagcaggcca aagccaatca caccgagcag
    51301 ccagatatcg gaaccgagcg gcagctgttg catacgcata tccagtaggc acagatccag
    51361 cgtgccataa cgctgccaca gcagccagca agcaatcacc agcagcagag tgccaagacg
    51421 ccccagcgca aaccacagtt tgccctcttt gctgttgctg gtgaggaaca ccgcacacaa
    51481 ggccatgact tcggccatta ccacgaacat gccgaggttg ctggcgatga cggcgcagac
    51541 ggcggcagcc atcaacatat tgatctgcaa gccgttgcac ttcacctgcg cgtggcgatg
    51601 ccagtcaatg ttgtagaggc tgacaaacag accgcacaga ccgagcgtaa tcagccaaat
    51661 cgcgttaagc ggagagattt gcacatcgta gcttaccagc gacagcgcac cgctcacgcc
    51721 aaccgcgcca gtcagtacag tgaagcccgc ggctgccgta tacagactac caaccgcgcc
    51781 gccaattcca gctatccagc cacttaacgc tttttgaaaa gaaaagagaa atgccagaac
    51841 agcggcggcg acaaaccatg ccacgccgct attgatcagg gaaattgcgc tcatttagcc
    51901 tctccacttt gagcctgctg aaacaaggtg agatcgccaa agtccgtgtt aaaggtcagc
    51961 tcacgcttac gtttgctgac gcgggcgata tcggtgttat ccaccagatg cagggcttta
    52021 gtcgggcaca tccgcacgca ggccggacct tgttcatcaa aactgcaaag gtcacatttg
    52081 acggcgatcg cgcgaatacc cggcacccag tcaagcagcg tactgatacg cgccggagca
    52141 ggcggtgccg gtggcgcttt cggggtattg gcgtttgccg gaatatccag cggacggctg
    52201 ccggaaaatt caattgcgcc aaacgggcag gcgatgccgc acagtttgca gcttacgcac
    52261 agactttcat tcaactgcac ggccccatca atgcgggtga tggcgttaac cgggcaaacc
    52321 accgcacagg gtgcatcttc acagtggtga cagagctgcg gcgcagattc tttttcattc
    52381 agcatcactc tcaggcgcgg cattgattgc aggccgtgct ggcgatgcgt ctctgaacag
    52441 gcggcctcac aagtgtggca gccgatacag agcgtggagt caacaattac aaaacgattc
    52501 accaggcatt cctcaggtga ttgtcatttt tgacgaaaac atgccgatga aatgtcattt
    52561 tcgacactca tcgacacgcc catccccaaa caggcgtaac gcctgcaaaa cgggcaaagc
    52621 ctcagctcat gctgccgggc tttgtccctt taccagttgg cttaaattca ccggcacatt
    52681 gttttcaata gcggtgtata agctgtcgta aacgccagcg attttttcga gatagtgttc
    52741 cagaacctgt tcgcgatcgc ggatgttgac cttgccatca atcatgccgt caatgctcag
    52801 ttgcgcctgc gcaatccgct gtttgtcttc gccatctttt gtttcgacgt aatactcgat
    52861 ggtgtggctg ttaaagccat ccgccagggt gacgtaaata cgaaaatgtt caaaaaggac
    52921 gaaacagagt tctttgtccg gcgcgcagct catggcgtga tgcaggcgcg ctttcgataa
    52981 cgtgatcccc tgaaccagcg aattgcagta gatgtgccac tggtcctgta ggcgacgatg
    53041 ccgctgtgcg atgtaatcgg ctttctcgct tatttcccaa atagtcattg tcaggttacc
    53101 cgtttaacag agatgccagc tttaagcatt ttctatgcca acttttaatt tattgttatt
    53161 aaatagattt ttaagctaaa gatgaatttc gtcgccgtgt cgacgtgtca tttcgacatc
    53221 atcgacatta ttcaccgcag ggataatcaa cactggcaca attattgctt gtagctggca
    53281 atagttaatg ggaggcgata tgcacgaaat aaccctctgc caacgggcac tggaattgat
    53341 cgaacagcag gccgcaaaac acggcgcaaa acgcgtaact ggggtctggc tcaaaattgg
    53401 cgcattttct tgtgtcgaaa ccagctctct tgccttttgt tttgatctgg tttgccgcgg
    53461 cagcgtggcg gaaggttgta aactgcacct cgaagaacaa gaggccgaat gctggtgtga
    53521 aacatgccaa cagtatgtga cgctactgac ccagcgcgtc cgccgctgtc cacagtgtca
    53581 tggtgacatg ctacagattg tggcagacga cggtttacag attcggcgga tagaaataga
    53641 ccaggagtga gcgatgtgta caacatgcgg ttgcggtgaa ggcaacctgt atatcgaggg
    53701 tgatgaacat aaccctcatt ccgcgtttcg tagcgcgcca tttgccccgg cggcacgccc
    53761 gaagatgaaa atcaccggca ttaaagcgcc tgaatttacc cccagccaga ctgaagaagg
    53821 cgacctgcat tacggtcatg gcgaagcggg cactcacgcg ccgggcatga gccagcgtcg
    53881 gatgctggaa gtcgaaattg acgtgctgga caaaaataac cgtctggctg aacgcaaccg
    53941 cgcgcgcttt gctgcccgca agcaactggt gctcaacctg gtttccagcc ctggttccgg
    54001 taaaaccacc ctgctgacgg aaaccctaat gcgcctgaaa gacagcgttc cgtgcgcggt
    54061 tattgaaggt gaccagcaaa ccgtgaacga tgccgcacgc attcgcgcta ccggcacacc
    54121 agcgattcag gtgaacaccg gtaaaggctg ccatcttgac gcacagatga ttgccgacgc
    54181 cgcaccgcgt ctgccactgg acgataacgg tattctgttt atcgaaaacg ttggcaacct
    54241 cgtatgcccg gccagcttcg atctcggtga aaaacacaaa gtggcggtgc tttccgttac
    54301 cgaaggtgaa gacaaaccgc tgaaatatcc gcatatgttt gccgccgcct cgctgatgct
    54361 gctcaacaaa gttgacctgt tgccgtatct caactttgac gttgagaagt gcatcgcctg
    54421 cgcccgcgaa gtcaatccag aaattgaaat catccttatt tccgccacca gcggcgaagg
    54481 gatggaccag tggctgaact ggctggagac acagcgatgt gcataggcgt tcccggccag
    54541 atccgcacca ttgacggcaa ccaggcaaaa gtcgacgtct gcggcattca gcgcgatgtc
    54601 gatttaacgt tagtcggcag ctgcgatgaa aacggtcagc cgcgcgtggg ccagtgggta
    54661 ctggtacacg ttggctttgc catgagcgta attaatgaag ccgaagcacg cgacactctc
    54721 gacgccttac aaaacatgtt tgacgttgag ccggatgtcg gcgcgctgtt gtatggcgag
    54781 gaaaaataat gcgttttgtt gatgaatatc gcgcgccgga acaggtgatg cagttaattg
    54841 agcatctgcg cgaacgtgct tcacatctct cttacaccgc cgaacgccct ctgcggatta
    54901 tggaagtgtg cggtggtcat acccacgcca tttttaaatt cggcctcgac cagttactgc
    54961 ctgaaaacgt tgagtttatc cacggtccgg gttgcccggt gtgcgtactg ccgatgggta
    55021 gaatcgacac ctgcgtggag attgccagcc atccggaagt catcttctgt acctttggcg
    55081 acgccatgcg cgtgccgggg aaacagggat cgctgttgca ggcaaaagca cgcggtgccg
    55141 atgtgcgcat cgtttactcg ccgatggatg cgttgaaact ggcgcaggag aatccaaccc
    55201 gtaaagtcgt attcttcggc ttaggttttg aaaccaccat gccgaccacc gccatcaccc
    55261 tgcaacaggc gaaagcccgc gatgtgcaga atttttactt cttctgccag catattacgc
    55321 ttatcccgac gctgcgcagt ttgctggaac agccggataa cggtatcgac gcgttcctcg
    55381 cgccgggcca cgttagtatg gttatcggca ctgatgccta taattttatc gccagcgatt
    55441 ttcatcgtcc gctggtggtc gctggtttcg aaccccttga tctactgcaa ggcgtggtca
    55501 tgctggtgga gcagaaaata gcggcccaca gcaaggtaga gaatcagtat cgtcgggtgg
    55561 taccggatgc cggtaacctg ctggcgcaac aggcaattgc tgatgtgttc tgtgtcaacg
    55621 gcgacagcga atggcgcggc ttaggcgtga ttgaatcttc tggcgtgcac ctgacgccgg
    55681 attatcaacg attcgatgcc gaagcacatt tccgcccagc accgcagcag gtctgcgatg
    55741 acccgcgcgc gcgttgtggt gaggtattaa cgggcaaatg taagccgcat caatgcccgc
    55801 tgtttggtaa cacctgtaat cctcaaaccg catttggtgc actgatggtc tcctccgaag
    55861 gagcgtgcgc cgcgtggtat cagtatcgtc agcaggagaa tgaagcgtga ataatatcca
    55921 actcgcccac ggcagcggcg gccaggcgat gcagcaatta atcaacagcc tgtttatgga
    55981 agcctttgcc aacccgtggc tggcagagca ggaagaccag gcacgtcttg atctggcgca
    56041 gctggtagca gaaggcgacc gtctggcgtt ctccaccgac agttacgtca ttgacccgct
    56101 gttctttcct ggcggtaata tcggcaagct ggcgatttgc ggcacagcca atgacgttgc
    56161 ggtcagtggc gctattccgc gctatctctc ctgtggcttt atcctcgaag aaggattgcc
    56221 gatggagaca ctgaaagccg tagtgaccag catggcagaa accgcgcgcg cggcaggcat
    56281 tgccatcgtt actggcgata ctaaagtggt gcagcgcggc gcggcagata aactgtttat
    56341 caacaccgct ggcatgggcg caattccggc gaatattcac tggggcgcac agacgctaac
    56401 cgcaggcgat gtattactgg ttagcggcac gctcggggac cacggggcga ctatccttaa
    56461 cctgcgtgag cagctggggc tggatggcga actggtcagc gactgcgcgg tactgacgcc
    56521 gcttattcag acgctgcgtg acattcccgg cgtgaaagcg ctgcgtgatg ccacccgtgg
    56581 tggtgtgaac gcggtagttc atgagttcgc ggcagcctgc ggttgtggta ttgaaatttc
    56641 agaagtggca ctgccggtta aacctgccgt gcgcggcgtt tgcgaactgc tgggactgga
    56701 cgcccttaac tttgccaacg aaggcaaact ggtgatcgcc gttgaacgca acgcggcaga
    56761 gcaagtgctg gcagcgttac attcccatcc actggggaaa gacgcggcgc taattggtga
    56821 agtggtggaa cgtaaaggtg ttcgtcttgc cggtctgtat ggtgtgaaac gaaccctcga
    56881 tttaccacac gccgaacctc ttccgcgtat atgctaataa aattctaaat ctcctatagt
    56941 tagtcaatga ccttttgcac cgctttgcgg tgctttcctg gaagaacaaa atgtcatata
    57001 caccgatgag tgatctcgga cagcaagggt tgttcgacat cactcggaca ctattgcagc
    57061 agcccgatct ggcctcgctg tgtgaggctc tttcgcaact ggtaaagcgt tctgcgctcg
    57121 ccgacaacgc ggctattgtg ttgtggcaag cgcagactca acgtgcgtct tattacgcat
    57181 cgcgtgaaaa agacacccca ttaaatatga agacgaaact gttctggcac acggtccggt
    57241 acgccgcatt ttgtcgcgcc ctgatacgct gcattgcagt tacgaagaat tttgtgaaac
    57301 ctggccgcag ctggtcgcag gtgggctata ccccaaattt ggtcactatt gcctgatgcc
    57361 actggcggcg gaagggcata tttttggtgg ctgtgaattt attcgttatg acgatcgtcc
    57421 ctggagcgaa aaagagttca atcgtctgca aacatttacg cagatcgttt ctgtcgtcac
    57481 cgaacaaatc cagagccgcg tcgtcaacaa tgtcgactat gagttgttat gccgggagcg
    57541 cgataacttc cgcatcctgg ttgccattac caacgcggtg ctttcccgcc tggatatgga
    57601 cgaactggtc agcgaagtcg ccaaagaaat ccattactat ttcgacattg acgatatcag
    57661 tatcgtctta cgcagccacc gtaaaaacaa actcaacatc tactccactc actatcttga
    57721 taaacaacat cccgcccacg aacagagcga agtcgatgaa gccggaaccc tcaccgaacg
    57781 cgtgttcaaa agtaaagaga tgctgctgat caatctccac gagcgggacg atttagcccc
    57841 ctatgaacgc atgttgttcg acacctaggg caaccagatt caaaccttgt gcctgttacc
    57901 gctgatgtct ggcgacacca tgctgggcgt gctgaaactg gcgcaatgcg aagagaaagt
    57961 gtttaccact accaatctga atttgctgcg ccagattgcc gaacgtgtgg caatcgctgt
    58021 cgataacgcc ctcgcctatc aggaaatcca tcgtctgaaa gaacggctgg ttgatgaaaa
    58081 cctcgccctg accgagcagc tcaacaatgt tgatagtgaa tttggcgaga ttattggccg
    58141 cagcgaagcc atgtacagcg tgcttaaaca agttgaaatg gtggcgcaaa gtgacagtac
    58201 cgtgctgatc ctcggtgaaa ctggcacggg taaagagctg attgcccgcg cgatccataa
    58261 tctcagtggg cgtaataatc gccgcatggt caaaatgaac tgcgcggcga tgcctgccgg
    58321 attgctggaa agcgatctgt ttggtcatga gcgtggggct tttaccggtg ccagcgccca
    58381 gcgcattggt cgttttgaac tggcggataa aagctctctg ttcctcgacg aagtgggcga
    58441 tatgccactg gagttacagc cgaagttgct gcgtgtattg caggaacagg agtttgaacg
    58501 tctcggcagc aacaaaatca ttcagacgga cgtacgttta attgccgcga ctaaccgcga
    58561 tctgaaaaaa atggtcgccg accgagagtt ccatagcgat ctctattacc gcctgaacgt
    58621 attcccgatt cacctgccgc cactacgcga gcgtccggaa gatattccgc tgctggcgaa
    58681 agcctttacc ttcaaaattg cccgtcgtct ggggcgcaat atcgacagca ttcctgccga
    58741 gacgctgcgc accttgagca acatggagtg gccgggtaac gtacgcgaac tggaaaacgt
    58801 cattgagcgc gcggtattgc taacacgcgg taacgtgctg cagctgtcat tgccagatat
    58861 tgttttaccg gaacctgaaa cgccgcctgc cgcaacggtt gtcgcccagg agggcgaaga
    58921 tgaatatcag ttgattgtgc gcgtgctgaa agaaaccaac ggcgtggttg ccgggcctaa
    58981 aggcgctgcg caacgtctgg ggctgaaacg cacgaccctg ctgtcacgga tgaagcggct
    59041 gggaattgat aaatcggcat tgatttaact gcaaattgcc ggacagatct gcctgtccgg
    59101 catactattc acgaggtttt ttcggacgat atttttccgg cagttctggt accggacgct
    59161 tgtcatcgat gagatgacgc acggttaaga tcggatgacg ccacagcatt ctcggcccgg
    59221 cccaacgcat aatctgtttc atctcttcac gctttgcagg ctggtaacag tgcaccggac
    59281 actgcttaca ggctggtttc tcttcgccga acacacattt atccagccgc ttttgcgcgt
    59341 aaacaaacaa cgcctcgtaa tgctccggct ccgctgacgc ctgcgggcat ttcgcttgat
    59401 aaagatcgat cattttttta atcgtcagtt tttcacgaga gatacgcttg ccggacatgc
    59461 tgcctccata ctcttaaggt gtatttatat tacaacttaa tttgaaaggg gactatggct
    59521 ctgaaggagg aggtttattc aaccgacgca attttgcgta cttgcttcat aagcatcacg
    59581 caaaagctgc aaaacagcat ctttcccgga accagcatca agaactcgcc gttcgcttct
    59641 tcccctgaaa tgattaactc cggtatcatg tgcgccttat gtgattacaa cgaaaataaa
    59701 aaccatcaca ccccatttaa tatcagggaa ccggacataa ccccatgagt gcaatagaaa
    59761 atttcgacgc ccatacgccc atgatgcagc agtatctcag gctgaaagcc cagcatcccg
    59821 agatcctgct gttttaccgg atgggtgatt tttatgaact gttttatgac gacgcaaaac
    59881 gcgcgtcgca actgctggat atttcactga ccaaacgcgg tgcttcggcg ggagagccga
    59941 tcccgatggc ggggattccc taccatgcgg tggaaaacta tctcgccaaa ctggtgaatc
    60001 agggagagtc cgttgccatc tgcgaacaaa ttggcgatcc ggcgaccagc aaaggtccgg
    60061 ttgagcgcaa agttgtgcgt atcgttacgc caggcaccat cagcgatgaa gccctgttac
    60121 aggagcgtca ggacaacctg ctggcggcta tctggcagga cagcaaaggt ttcggctacg
    60181 cgacgctgga tatcagctcc gggcgttttc gcctgagcga accggccgac cgcgaaacga
    60241 tggcggcaga gctgcaacgc actaatccgg cggaactgct gtatgcagaa gatttcgccg
    60301 agatgtcgct gattgaaggc cgtcgcggcc tgcgccgtcg cccgctgtgg gagtttgaaa
    60361 tcgacaccgc gcgccagcag ttgaatctgc aatttggcac ccgcgatctg gtcggttttg
    60421 gcgtcgagaa cgcgccgcgc ggactttgtg ctgccggttg tctgttgcag tatgcgaaag
    60481 atacccaacg cacgaccctg ccgcatattc gttctatcac catggaacgt gagcaggaca
    60541 gcatcattat ggatgccgcg acacgtcgta acctggaaat tactcagaac ctggcgggcg
    60601 gtgcggaaaa tacgctggct tctgtgctcg actgcaccgt cacgccgatg ggtagtcgta
    60661 tgctgaaacg ctggctgcat atgccagtgc gcgatacccg cgtgttgctt gagcgccagc
    60721 aaactattgg cgcattgcag gatttcaccg ccgagttgca gccggtactg cgtcaggtcg
    60781 gcgacctgga acgtattctg gcacgtctgg ctttacgaac tgctcgccca cgcgatctgg
    60841 cccgtatgcg ccacgctttc cagcaactgc cggagctgcg tgcgcagtta gaaactgtcg
    60901 atagtgcacc ggtacaggcg ctacgtgaga agatgggcga gtttgccgag ctgcgcgatc
    60961 tgctggagcg agcaatcatc gacacaccgc cggtgctggt acgcgacggt ggtgttatcg
    61021 catcgggcta taacgaagag ctggatgagt ggcgcgcgct ggctgacggc gcgaccgatt
    61081 atctggagcg tctggaagtc cgcgagcgtg aacgtaccgg cctggacacg ctgaaagttg
    61141 gctttaatgc ggtgcacggc tactacattc aaatcagccg tgggcaaagc catctggcac
    61201 ccatcaacta catgcgtcgc cagacgctga aaaacgccga gcgctacatc attccagagc
    61261 taaaagagta cgaagataaa gttctcacct caaaaggcaa agcactggca ctggaaaaac
    61321 agctttatga agagttgttc gacctgctgt tgccgcatct ggaagcgttg caacagagcg
    61381 cgagcgcgct ggcggaactc gacgtgctgg tgaacctggc ggaacgggcc tataccctga
    61441 actacacctg cccgaccttc attgataaac cgggcattcg cattaccgaa ggtcgccatc
    61501 cggtagttga acaagtactg aatgagccat ttatcgctaa cccgctgaat ctgtcgccgc
    61561 agcgccgtat gttgatcatc accggtccga acatgggcgg taaaagtacc tatatgcgcc
    61621 agaccgcact gattgcgctg atggcctaca tcggcagcta tgtaccggca caaaaagtcg
    61681 agattggacc tatcgatcgc atctttaccc gcgtaggcgc ggcagatgac ctggcttccg
    61741 ggcgctcaac ctttatggtg gagatgactg aaaccgccaa tattttacat aacgccaccg
    61801 aatacagtct ggtgttaatg gatgagatcg ggcgtggaac gtccacctac gatggtctgt
    61861 cgctggcgtg ggcgtgcgca gaaaatctgg cgaataagat taaggcattg acgctgtttg
    61921 ccacccacta tttcgagctg acccagctac cggagaaaat ggaaggcgtc gccaacgtac
    61981 atctcgatgc gctggagcac ggcgacacta tcgcctttat gcacagcgtg caggacggcg
    62041 cggcgagcaa aagctacggt ctggcggttg ctgctctggc gggcgtaccg aaagaggtta
    62101 ttaagcgcgc acggcaaaaa ctgcgtgagc tggaaagcat ttcgccgaac gccgccgcga
    62161 cgcaagtgga tggtacgcag atgtctttgc tgtcagtgcc agaagaaact tcgcctgcgg
    62221 tcgaagcact ggaaaatctc gacccggatt cactgactcc gcgtcaggcg ctggagtgga
    62281 tttatcgctt gaagagtctg gtgtaataac aattcccgat agtcttttgc tatcgggaat
    62341 attaacgaca actgacgaat caaataaaaa taccctgtat aataggaaag cttattttac
    62401 agggtaaaac catgccatct acacgctatc aaaaaatcaa tgcccatcac tatcgccata
    62461 tatgggtcgt tggtgatatt catggtgaat atcagttatt acaatcccgt taacatcaac
    62521 tctcttttta ccctgaaacc gacttactta tttccacagg cgataatatt gatcgtgggc
    62581 cgaagagtct taacgtgctg cgcctgctaa accaaccctg gtttacgtcg gttaaaggca
    62641 accacgaagc gatggcgttg gatgcattcg aaactggcga tggaaatatg tggcttgcca
    62701 gcggtggtga ctggtttttc gatttaaatg attcagagca acaagaggca atagatctgt
    62761 tgctgaaatt ccatcacctt ccacatatta ttgaaatcat taatgacaac ataaaatatg
    62821 ccatc
//
LOCUS       DAZPJP010000005        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPJP010000005 DAZPJP010000000
VERSION     DAZPJP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.005"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHU9J_000262"
     CDS             <1..468
                     /locus_tag="ACHU9J_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1903915.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHU9J_000263"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHU9J_000263"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1903916.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHU9J_000264"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHU9J_000264"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1903917.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHU9J_000265"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHU9J_000265"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1903918.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHU9J_000266"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHU9J_000266"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1903919.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHU9J_000267"
     CDS             complement(4084..5313)
                     /locus_tag="ACHU9J_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1903920.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHU9J_000268"
     CDS             complement(5377..5817)
                     /locus_tag="ACHU9J_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1903921.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHU9J_000269"
     CDS             complement(5922..6692)
                     /locus_tag="ACHU9J_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1903922.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHU9J_000270"
     CDS             complement(6689..7615)
                     /locus_tag="ACHU9J_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1903923.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHU9J_000271"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHU9J_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1903924.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHU9J_000272"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHU9J_000272"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1903925.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHU9J_000273"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHU9J_000273"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1903926.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHU9J_000274"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHU9J_000274"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1903927.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHU9J_000275"
     CDS             13322..13669
                     /locus_tag="ACHU9J_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1903928.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHU9J_000276"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHU9J_000276"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1903929.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHU9J_000277"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHU9J_000277"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1903930.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHU9J_000278"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHU9J_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1903931.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHU9J_000279"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHU9J_000279"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1903932.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHU9J_000280"
     CDS             18978..20735
                     /locus_tag="ACHU9J_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1903933.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHU9J_000281"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHU9J_000281"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1903934.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHU9J_000282"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHU9J_000282"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1903935.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHU9J_000283"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHU9J_000283"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1903936.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHU9J_000284"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHU9J_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1903937.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHU9J_000285"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHU9J_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1903938.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHU9J_000286"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHU9J_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1903939.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHU9J_000287"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHU9J_000287"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1903940.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHU9J_000288"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHU9J_000288"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1903941.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHU9J_000289"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHU9J_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1903942.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHU9J_000290"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHU9J_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR1903943.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHU9J_000291"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHU9J_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1903944.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHU9J_000292"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHU9J_000292"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1903945.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHU9J_000293"
     CDS             complement(36854..36898)
                     /locus_tag="ACHU9J_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1903946.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHU9J_000294"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHU9J_000294"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1903947.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHU9J_000295"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHU9J_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1903948.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHU9J_000296"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHU9J_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1903949.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHU9J_000297"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHU9J_000297"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1903950.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHU9J_000298"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHU9J_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1903951.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHU9J_000299"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHU9J_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1903952.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHU9J_000300"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHU9J_000300"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1903953.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHU9J_000301"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHU9J_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1903954.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHU9J_000302"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHU9J_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1903955.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHU9J_000303"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHU9J_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1903956.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHU9J_000304"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHU9J_000304"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1903957.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHU9J_000305"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHU9J_000305"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1903958.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHU9J_000306"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHU9J_000306"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1903959.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHU9J_000307"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHU9J_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1903960.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHU9J_000308"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHU9J_000308"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1903961.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHU9J_000309"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHU9J_000309"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1903962.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHU9J_000310"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHU9J_000310"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1903963.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHU9J_000311"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHU9J_000311"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1903964.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHU9J_000312"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHU9J_000312"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1903965.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHU9J_000313"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHU9J_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1903966.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHU9J_000314"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHU9J_000314"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1903967.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHU9J_000315"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHU9J_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1903968.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14597 a        16851 c        15000 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgaca gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZPJP010000006        54244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPJP010000006 DAZPJP010000000
VERSION     DAZPJP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.006"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(396..482)
                     /locus_tag="ACHU9J_000316"
     tRNA            complement(396..482)
                     /locus_tag="ACHU9J_000316"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Leu,seq:gag)
     gene            complement(497..829)
                     /gene="secG"
                     /locus_tag="ACHU9J_000317"
     CDS             complement(497..829)
                     /gene="secG"
                     /locus_tag="ACHU9J_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1903969.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1057..2394)
                     /gene="glmM"
                     /locus_tag="ACHU9J_000318"
     CDS             complement(1057..2394)
                     /gene="glmM"
                     /locus_tag="ACHU9J_000318"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1903970.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2387..3235)
                     /gene="folP"
                     /locus_tag="ACHU9J_000319"
     CDS             complement(2387..3235)
                     /gene="folP"
                     /locus_tag="ACHU9J_000319"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1903971.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3325..5259)
                     /gene="ftsH"
                     /locus_tag="ACHU9J_000320"
     CDS             complement(3325..5259)
                     /gene="ftsH"
                     /locus_tag="ACHU9J_000320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1903972.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5359..5988)
                     /gene="rlmE"
                     /locus_tag="ACHU9J_000321"
     CDS             complement(5359..5988)
                     /gene="rlmE"
                     /locus_tag="ACHU9J_000321"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1903973.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6114..6407
                     /gene="yhbY"
                     /locus_tag="ACHU9J_000322"
     CDS             6114..6407
                     /gene="yhbY"
                     /locus_tag="ACHU9J_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1903974.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6563..7039)
                     /gene="greA"
                     /locus_tag="ACHU9J_000323"
     CDS             complement(6563..7039)
                     /gene="greA"
                     /locus_tag="ACHU9J_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1903975.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7287..8720
                     /gene="dacB"
                     /locus_tag="ACHU9J_000324"
     CDS             7287..8720
                     /gene="dacB"
                     /locus_tag="ACHU9J_000324"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1903976.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            8775..8882
                     /locus_tag="ACHU9J_000325"
                     /pseudo
     CDS             8775..8882
                     /locus_tag="ACHU9J_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8906..10078)
                     /gene="cgtA"
                     /locus_tag="ACHU9J_000326"
     CDS             complement(8906..10078)
                     /gene="cgtA"
                     /locus_tag="ACHU9J_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1903977.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     KPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10094..11059)
                     /locus_tag="ACHU9J_000327"
     CDS             complement(10094..11059)
                     /locus_tag="ACHU9J_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1903978.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11186..11443)
                     /gene="rpmA"
                     /locus_tag="ACHU9J_000328"
     CDS             complement(11186..11443)
                     /gene="rpmA"
                     /locus_tag="ACHU9J_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1903979.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11464..11775)
                     /gene="rplU"
                     /locus_tag="ACHU9J_000329"
     CDS             complement(11464..11775)
                     /gene="rplU"
                     /locus_tag="ACHU9J_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1903980.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12034..13005
                     /gene="ispB"
                     /locus_tag="ACHU9J_000330"
     CDS             12034..13005
                     /gene="ispB"
                     /locus_tag="ACHU9J_000330"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1903981.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13233..13511
                     /gene="sfsB"
                     /locus_tag="ACHU9J_000331"
     CDS             13233..13511
                     /gene="sfsB"
                     /locus_tag="ACHU9J_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1903982.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13559..14818)
                     /gene="murA"
                     /locus_tag="ACHU9J_000332"
     CDS             complement(13559..14818)
                     /gene="murA"
                     /locus_tag="ACHU9J_000332"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1903983.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14873..15127)
                     /gene="ibaG"
                     /locus_tag="ACHU9J_000333"
     CDS             complement(14873..15127)
                     /gene="ibaG"
                     /locus_tag="ACHU9J_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1903984.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15287..15580)
                     /gene="mlaB"
                     /locus_tag="ACHU9J_000334"
     CDS             complement(15287..15580)
                     /gene="mlaB"
                     /locus_tag="ACHU9J_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1903985.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15580..16215)
                     /gene="mlaC"
                     /locus_tag="ACHU9J_000335"
     CDS             complement(15580..16215)
                     /gene="mlaC"
                     /locus_tag="ACHU9J_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1903986.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16234..16785)
                     /gene="mlaD"
                     /locus_tag="ACHU9J_000336"
     CDS             complement(16234..16785)
                     /gene="mlaD"
                     /locus_tag="ACHU9J_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1903987.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16790..17572)
                     /gene="mlaE"
                     /locus_tag="ACHU9J_000337"
     CDS             complement(16790..17572)
                     /gene="mlaE"
                     /locus_tag="ACHU9J_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1903988.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17580..18389)
                     /gene="mlaF"
                     /locus_tag="ACHU9J_000338"
     CDS             complement(17580..18389)
                     /gene="mlaF"
                     /locus_tag="ACHU9J_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1903989.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18599..19576
                     /locus_tag="ACHU9J_000339"
     CDS             18599..19576
                     /locus_tag="ACHU9J_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1903990.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19590..20576
                     /gene="kdsD"
                     /locus_tag="ACHU9J_000340"
     CDS             19590..20576
                     /gene="kdsD"
                     /locus_tag="ACHU9J_000340"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1903991.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20597..21163
                     /gene="kdsC"
                     /locus_tag="ACHU9J_000341"
     CDS             20597..21163
                     /gene="kdsC"
                     /locus_tag="ACHU9J_000341"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1903992.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21160..21735
                     /gene="lptC"
                     /locus_tag="ACHU9J_000342"
     CDS             21160..21735
                     /gene="lptC"
                     /locus_tag="ACHU9J_000342"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1903993.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21704..22261
                     /gene="lptA"
                     /locus_tag="ACHU9J_000343"
     CDS             21704..22261
                     /gene="lptA"
                     /locus_tag="ACHU9J_000343"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1903994.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22268..22993
                     /gene="lptB"
                     /locus_tag="ACHU9J_000344"
     CDS             22268..22993
                     /gene="lptB"
                     /locus_tag="ACHU9J_000344"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1903995.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23041..24474
                     /gene="rpoN"
                     /locus_tag="ACHU9J_000345"
     CDS             23041..24474
                     /gene="rpoN"
                     /locus_tag="ACHU9J_000345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1903996.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24497..24784
                     /gene="hpf"
                     /locus_tag="ACHU9J_000346"
     CDS             24497..24784
                     /gene="hpf"
                     /locus_tag="ACHU9J_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1903997.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24902..25393
                     /gene="ptsN"
                     /locus_tag="ACHU9J_000347"
     CDS             24902..25393
                     /gene="ptsN"
                     /locus_tag="ACHU9J_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1903998.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25439..26293
                     /gene="rapZ"
                     /locus_tag="ACHU9J_000348"
     CDS             25439..26293
                     /gene="rapZ"
                     /locus_tag="ACHU9J_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1903999.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26290..26562
                     /gene="npr"
                     /locus_tag="ACHU9J_000349"
     CDS             26290..26562
                     /gene="npr"
                     /locus_tag="ACHU9J_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1904000.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26776..27408
                     /gene="yrbL"
                     /locus_tag="ACHU9J_000350"
     CDS             26776..27408
                     /gene="yrbL"
                     /locus_tag="ACHU9J_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1904001.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27405..28133)
                     /gene="mtgA"
                     /locus_tag="ACHU9J_000351"
     CDS             complement(27405..28133)
                     /gene="mtgA"
                     /locus_tag="ACHU9J_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1904002.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28130..28783)
                     /gene="elbB"
                     /locus_tag="ACHU9J_000352"
     CDS             complement(28130..28783)
                     /gene="elbB"
                     /locus_tag="ACHU9J_000352"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1904003.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29013..31349)
                     /gene="arcB"
                     /locus_tag="ACHU9J_000353"
     CDS             complement(29013..31349)
                     /gene="arcB"
                     /locus_tag="ACHU9J_000353"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1904004.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31445..32374)
                     /locus_tag="ACHU9J_000354"
     CDS             complement(31445..32374)
                     /locus_tag="ACHU9J_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1904005.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33049..37509
                     /gene="gltB"
                     /locus_tag="ACHU9J_000355"
     CDS             33049..37509
                     /gene="gltB"
                     /locus_tag="ACHU9J_000355"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1904006.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37522..38940
                     /gene="gltD"
                     /locus_tag="ACHU9J_000356"
     CDS             37522..38940
                     /gene="gltD"
                     /locus_tag="ACHU9J_000356"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1904007.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39124..39528
                     /locus_tag="ACHU9J_000357"
                     /pseudo
     CDS             39124..39528
                     /locus_tag="ACHU9J_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            39620..39925
                     /locus_tag="ACHU9J_000358"
     CDS             39620..39925
                     /locus_tag="ACHU9J_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1904008.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(40001..40465)
                     /gene="nanQ"
                     /locus_tag="ACHU9J_000359"
     CDS             complement(40001..40465)
                     /gene="nanQ"
                     /locus_tag="ACHU9J_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1904009.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40462..41337)
                     /gene="nanK"
                     /locus_tag="ACHU9J_000360"
     CDS             complement(40462..41337)
                     /gene="nanK"
                     /locus_tag="ACHU9J_000360"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1904010.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41334..42023)
                     /locus_tag="ACHU9J_000361"
     CDS             complement(41334..42023)
                     /locus_tag="ACHU9J_000361"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1904011.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42071..43561)
                     /gene="nanT"
                     /locus_tag="ACHU9J_000362"
     CDS             complement(42071..43561)
                     /gene="nanT"
                     /locus_tag="ACHU9J_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1904012.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43671..44564)
                     /gene="nanA"
                     /locus_tag="ACHU9J_000363"
     CDS             complement(43671..44564)
                     /gene="nanA"
                     /locus_tag="ACHU9J_000363"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1904013.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44686..45477)
                     /gene="nanR"
                     /locus_tag="ACHU9J_000364"
     CDS             complement(44686..45477)
                     /gene="nanR"
                     /locus_tag="ACHU9J_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1904014.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            45857..47179
                     /gene="dcuC"
                     /locus_tag="ACHU9J_000365"
     CDS             45857..47179
                     /gene="dcuC"
                     /locus_tag="ACHU9J_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1904015.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(47222..47719)
                     /gene="sspB"
                     /locus_tag="ACHU9J_000366"
     CDS             complement(47222..47719)
                     /gene="sspB"
                     /locus_tag="ACHU9J_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1904016.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47725..48363)
                     /gene="sspA"
                     /locus_tag="ACHU9J_000367"
     CDS             complement(47725..48363)
                     /gene="sspA"
                     /locus_tag="ACHU9J_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1904017.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(48758..49150)
                     /gene="rpsI"
                     /locus_tag="ACHU9J_000368"
     CDS             complement(48758..49150)
                     /gene="rpsI"
                     /locus_tag="ACHU9J_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1904018.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(49166..49594)
                     /gene="rplM"
                     /locus_tag="ACHU9J_000369"
     CDS             complement(49166..49594)
                     /gene="rplM"
                     /locus_tag="ACHU9J_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1904019.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(49813..50940)
                     /gene="zapE"
                     /locus_tag="ACHU9J_000370"
     CDS             complement(49813..50940)
                     /gene="zapE"
                     /locus_tag="ACHU9J_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1904020.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            51134..51532
                     /gene="zapG"
                     /locus_tag="ACHU9J_000371"
     CDS             51134..51532
                     /gene="zapG"
                     /locus_tag="ACHU9J_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1904021.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51686..53053
                     /gene="degQ"
                     /locus_tag="ACHU9J_000372"
     CDS             51686..53053
                     /gene="degQ"
                     /locus_tag="ACHU9J_000372"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1904022.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            53143..54210
                     /gene="degS"
                     /locus_tag="ACHU9J_000373"
     CDS             53143..54210
                     /gene="degS"
                     /locus_tag="ACHU9J_000373"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1904023.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        13340 a        14572 c        13530 g        12802 t
ORIGIN      
        1 atgtttttca actctgatcc atatcagacc gaaacactgt tagttttttc tttttaaatg
       61 atgcggccat tgcgttctca gtaattaaat aacattatca atgcgtctct aaacattacg
      121 cagaacgtag ccatcagtag ttatactgac aggagcagga tggtttatcg agaagtaaac
      181 atatccgtat tcgtaccgga actggagttg acacaataaa gtgccaatta tgtcagcaga
      241 agggaaaagt ttataactaa ggcgtaccgg caccatcgtt tcaaggtacc agctacgagt
      301 aaagcaactg gacgagatac agatacctga caaccattcc tcagaccagg accaaaacga
      361 aaaaagacgc ttttcagcgt ctcttttctg gaatttggta ccgaggacgg gacttgaacc
      421 cgtaagccct attgggcact accacctcaa ggtagcgtgt ctaccaattc caccacctcg
      481 gcacggatac tactttttag ttcgggatat cgctggtcgg cttagccgga gcagctggct
      541 gagtttgttc ggttttcgcc ggtgcactca gattttccca ttcgctacct ttattggttt
      601 tgttgctatt gatgttaccc agcaccagac tgatgatgaa gaataacgtt gccagcagcg
      661 ccgtcatgcg ggtcatgaag ttaccagaac cacttgaacc aaacagcgta gcggaagcgc
      721 ctgctccgaa ggaggctccc atatcagcgc ctttaccttg ctgcagcatg atcagaccaa
      781 caaggccaat tgccacaata aggaaaacta ccaaaagagc ttcgtacata atcaacctgt
      841 tccttgcgga gttgccgcgt accaatgctt caaccaataa agcgggagtt tttaatcttt
      901 cccactgaag tgggtgtgaa tactaaccaa agcgaatgac cttcgcaagg gcaattttat
      961 cgcattgtat caactgcgga aaaaaacagc aaaaccgatg tgttcgctga gaaaaaaggc
     1021 gacaatcgcc gcctttttag ccagttattt accgttttaa acggctttta ctgcatcggc
     1081 gatacggtgt gcaaattcag ttacctgcgc ttcgtcttca ccttctacca tcacgcgaat
     1141 taacggttcg gtgccggatt tacgcaacag cacacgtcca cggctaccca gcgccgcttc
     1201 aacttcggcg gtcacagctt taactgactc atgctcaagc ggatcgccgc tacctgcggt
     1261 gtaacgcacg ttaaccagaa tctgcgggaa cattttcatg ccgctgcaaa ggtcgtgcag
     1321 gctcatatgg ttacgtgcca tcgccgccag cacctgcaag ccagcaacga tgccgtcacc
     1381 ggtagtggtt ttatccagca ggatcacatg accggaattc tctgcaccga tacgccagcc
     1441 tttctcctgc attttttcca gtacgtagcg gtcacccact ttcgcgcgcg caaatggaat
     1501 tcctaactgt ttcaacgcca gctcaagccc catgttactc atcaatgtac ccacagcacc
     1561 accacgcagc tggccctgac gaagaccttc acgcgcgatg atatacatga tctgatcacc
     1621 atcgacttta ttgccttcat ggtcaaccat aatcacgcga tcgccgtcgc cgtcgaaggc
     1681 aataccgaga tccgcttttt cagccagcac acgagcctgg agcgcgcgaa cgtcggtagc
     1741 ccccacttcg gcattgatgt ttacaccgtt cggctcacaa ccgatagcga taacgttcgc
     1801 ccccagttca cgcagcacgt tcggcgcgat gtgataagtc gcaccgtttg cgcaatccac
     1861 cacaatcttc agttcgctga ggctaagttc gttcgggaac gtggctttgc aaaactcgat
     1921 atagcgaccc gcagcatcaa cgatacggct ggctttaccc agttctgccg aatcaacaca
     1981 gctgatctct ttttccattt ccgcttcgat ggcctcttct accgcatccg gtagtttggt
     2041 gccgtcgata gagaaaaatt taatgccatt atcgtagaac gggttatgcg atgcagatat
     2101 cacaattccg gcctctgcgc ggaaggtacg cgtcagataa gccacggccg gtgttggcat
     2161 cgggccagtg aagagtgcgg aaaggcccgc tgccgccaga cccgcttcca gtgctgactc
     2221 cagcatatag ccagaaatac gcgtgtcttt accaataatg atcttacggg agccgtggcg
     2281 cgccagcact ttacccgcgg cccaacccag cttaagcaca aaatcaggtg tgatcggcgc
     2341 atcccctaca cgaccacgaa tcccatcggt accgaaatat ttacgattac tcatagcgtt
     2401 tgttttcctt tgcagacaga gtggcttcca ccacccgcat cgcttctacg gtttctttga
     2461 cgtcatgaac acgaataatg tgcgcgcctt gcattgcggc aatgaccgca caggccagac
     2521 taccgctcag gcgctcggac ggccccacgt tcagcagctg cccaatcatc gattttcgtg
     2581 acatacctac caacagcggc aggttgaaat ggtgaaattc agccaggcgc gccagtaatg
     2641 aatagttatg ggagagattt ttaccgaaac cgaatccggg gtcgagcaac aatttctctt
     2701 ttgcgatacc cgcctgctcg caacgtgcta tttgctcaat aaagtagcga ttcacttctg
     2761 caaagacatc gtcatacttc ggagcttcct gcatggtttt tggatttccc tgcatatgca
     2821 tcagacaaac cggtaaaccg gtttctgcag ccgcctccag agcgccaggt tcggaaaggg
     2881 agcggatatc gttaataatg tgagcgccaa ctttcgctga ctcacggatg acttctggtt
     2941 tggatgtatc taccgagatc cagacttcga agcgttgagc aattgcctca accacaggaa
     3001 taacacgttg caactcttct tcaacgctaa cttccgccgc ccctgggcgc gtggactcgc
     3061 caccaacgtc aatgatcgtc gcgccagcgt tgatcatcag attcgcatgt ttcaccgcat
     3121 ctatcagcga gttatgcgtg ccaccatccg aaaaggaatc aggcgtgacg ttgaggatcc
     3181 ccattacgtg aggatggcta aggtccagtg aagtgccctg ggcaaagagt ttcatggtgt
     3241 tatccctggt atgaattgat aagaaaaaac cccggagcac gccccggggt tttcggtaca
     3301 aatacagtca tctgatgcgg gaacttactt gtcgcctaac tgctctgaca tggtgttacc
     3361 cgggttcggc gtacgcggtt catcaaccgg acgaggagcc tttggactac cattgtcgcc
     3421 agaattgtta gaagcgcctg gttcttccca gcccgctggc ggacgtacat cgcgacgtgc
     3481 catcaggtca tcaatctgcg gtgcgtcgat agtctcatat ttcatgagag catctttcat
     3541 cgcatgcaga atatccatat tgtcggtcag aagctgacgc gcacgattat agttacgctc
     3601 aatcagtgct ttcacttcct ggtcgatgat acgtgcagtt tcatcggaca tatgtttcgc
     3661 tttcgctacg ctacggccga ggaacacttc accttcttct tccgcgtaca gcagtggacc
     3721 caatttctca gagaagcccc actgagtcac catgttacgc gccaggttgg tcgcaacttt
     3781 aatatcgttg gacgcaccgg tagaaacatg ttccggcccg tagatgatct cttctgccag
     3841 acgaccaccg tacagcgtag aaatctggct ttccagtttc tgacggctgg cgctgattgc
     3901 gtcgccctca ggcaagaaga aagtcacacc cagcgcacga ccgcgtggga taatcgtcac
     3961 tttgtgcacc ggatcgtgtt ccggcaccag gcgaccgata atcgcatgac ccgcttcgtg
     4021 gtaagccgtc gattctttct gcgcttccgt catcaccatg gagcgacgtt ccgcacccat
     4081 catgatttta tctttcgctt tctcgaactc aaccatcgac acaacgcgtt tgttgccacg
     4141 agcagcgaac agtgccgctt cgtttaccag gttcgccagg tcagcaccgg agaaaccagg
     4201 agtaccacgg gcaatgattg ccgcgtcgat atcgggtgcc aatggtacgc gacgcatgtg
     4261 aactttcagg atctgctcac gaccgcgaac atctggcaag ccaaccacaa cctgacggtc
     4321 gaaacggcca ggacgcagca gggccgggtc aagaacgtcc ggacggttag tcgcggcgat
     4381 aacgatgata ccttcgttac cttcgaagcc atccatctca accagcatct ggttcagagt
     4441 ctgttcacgt tcatcgtgac caccgcccag accagcgcca cgctggcggc ctacggcgtc
     4501 gatttcatcg ataaagatga tgcacggtgc cgctttcttc gcctgttcga acatgtcacg
     4561 aacacgggat gcacccacac cgacgaacat ttctacgaag tcagaaccgg agatagtaaa
     4621 gaacggaact ttcgcttcgc ctgcaatcgc tttcgccagc agcgttttac cggtacccgg
     4681 aggaccgacc atcaggacgc ctttcgggat cttaccgccg agtttctgga agcggctcgg
     4741 ctcgcgcaga tattcaacca gttcagcaac ttcttctttt gcttcgtcgc agcccgcaac
     4801 gtcagcaaag gtcgttttga tctgatcttc cgtcagcatg cgcgctttgc tcttaccaaa
     4861 cgacatggca cctttgccac cgccgccctg catttgacgc atgaagaaga tccacacacc
     4921 aatcagcaac agcatcggga accaggagat gaagatagaa gccagcaggc ttggttcttc
     4981 aggcggttca ccgacaacct tgacgttctt ggtcaacagg ttatccagta atttcggatc
     5041 ctgaaccgga atgtaagtgg tataacggtt actatctttc ttggtaacgt tgatttcacg
     5101 tccgttgata cgcgcttcac gaacctggtc gttattgacc tcttgtagga aggtagagta
     5161 atccacctta cggccattag actcgctggg cccaaagctc tggaatactg acatcagcac
     5221 aacggcaatg accagccaga gtattaggtt tttcgccatg tcactcaagg gattaacctc
     5281 ttattacaac tgtgttaaaa acagcgtcag gatactctat atccagcatc tttcaaactt
     5341 tcgtctgaaa tctcccggtt agggtttacg cccggtcgct acaatataca cttcccgcga
     5401 acgtgcacga gaagagtccg gcttacgaac tttgaccttc gtaaacaggg agcgaatttc
     5461 ccttagatac tcatcgaaac cttcgccctg gaacaccttc actacaaaac tgccacctgg
     5521 cgctaataca tcacgacaca tttctagcgc cagttccacc agatacatgg cacgggggat
     5581 atccaccgcc ggtgttccgc tcatgtttgg tgccatatcg gacatgacaa cctggacttt
     5641 gctgtcgcca acgcgctcca gcagtgcttt catcaccagt tcatcacgaa aatcgccctg
     5701 aagaaagtcc acaccaacga taggatccat aggtaaaaga tcgcaagcga tgatgcggcc
     5761 tttgccgcca atttgggtga ccacatattg tgaccaacca cccggagcag caccaaggtc
     5821 gacaaccgtc attcccggtt taaagagttt gtcactttgc tgtatttcat caagtttaaa
     5881 ccaggcacgg gaacgtaacc cctttttctg tgcctgttga acatatttat cgctaaagtg
     5941 ttcctgaagc cagcggctgg agctggcaga acgcttctta cctgtcattt aactttccca
     6001 tggggataac tcatcgtaac caattgcgta aatttttacg cgcctatttg gtgatatatg
     6061 ggagatggcg gtagaatgac ccattttcaa tcccaacgta agcaaaaaat acgatgaatc
     6121 tgagtactaa acaaaaacag cacctgaaag gtctggcaca tccgctcaag ccagttgttc
     6181 tgcttggcag taatggtttg accgaagggg tgctggccga gattgaacaa gcgttagagc
     6241 accatgaact catcaaggtg aaaatcgcca ccgaagatcg cgaaacaaaa accttgatcg
     6301 tggaagctat cgtgcgcgaa accggcgcct gtaatgtaca ggtcatcggt aaaacgctgg
     6361 tgctttatcg cccaactaaa gaacgtaaaa tctcgctgcc acgctaagat tatcctaaag
     6421 ttacacacat ttgctgtgta aaacgagggg ttttccgcag gcaggagagc aaaatgccac
     6481 gctctgttcg ttgataaaag gccgcatagc ggcctttttc ctttctttac aatacatcaa
     6541 catcttgagt attgggtaat tcttacaggt attccacctt aattacttca aattctactt
     6601 cgccgcccgg cgttttgatg accacaacat catcttcttc tttgccgatc aggccacgag
     6661 caataggcga gtttacagaa atcaggtttt gtttaaagtc agcttcgtca tcgccaacga
     6721 tgcgataagt ctgttcttcg tcagaatcca gattcagcac cgttacggta gcaccaaaaa
     6781 taacgcgccc attgttaggc attttggtga catcaatcac ctgcgcgttt gacagcttgg
     6841 cttcgatgtc tttgatacgg ccttcgcaga agccctgctg ttcacgagct gcgtggtatt
     6901 cggcgttttc tttcaggtcg ccatgctcac gcgcttccgc gatagcagca atgatttcag
     6961 gacggcgcac agatttcaga aaatccagct cttcgcgtaa tttttcagcg ccgcgtaagg
     7021 tcatcggaat agcttgcatt tgttatacct cttgaatatt cctgatgggg caagtcttca
     7081 cccaccccgg ctgttcggcc tgcccggcat aactcctcac cgggaccaga agcaaaaaaa
     7141 taccgaccca ggtacaagtc ccaggtcagc tacaattcac attttgatag tcattttacc
     7201 ctgaagttcc cgaagggtca tcgtttactt tatagggcgt tgcgccgtag tatgacggct
     7261 cgattccagg ttgttagcgc gagattatgc gattttccag atttatcatc ggattgacca
     7321 gctgtatagc gttcagtgtt caggccgcaa atgttgatga gtacattact caactccccg
     7381 ctggtgccaa ccttgccctg atggtgcaaa aagtcggcgc gtcggccccc gctattgatt
     7441 accacagtca gcagatggcg ctgcctgcca gtacccagaa agtgattact gcgctggcgg
     7501 cgttgattca actcggcccc gattttcgtt ttaccacgac gcttgaaacc aaaggcaatg
     7561 tggaaaacgg cgtacttaag ggtgacttag tggcgcgatt tggtgccgat ccgacgttaa
     7621 aacgtcagga tattcgcaat atggtcgcga ctttgaaaaa atctggcgtc aaccaaatcg
     7681 atggcaatgt gttgatagat acctccattt tcgccagcca cgataaagcc cccggctggc
     7741 catggaatga catgacacaa tgctttagcg ctccgcctgc cgccgccata gttgaccgca
     7801 actgtttctc cgtctcgctc tacagtgccc aaaaacccgg tgatatggcc tttatacgcg
     7861 tggcatctta ttaccccgtt acgatgttca gccaggtacg caccctcccc cgtggttctg
     7921 ccgaagcgca atactgcgaa ctggatgtcg taccaggcga cctgaaccgc tttacgctga
     7981 cgggatgcct gccacaacgt tctgagccgc tcccgttggc ttttgccgtg caggatggag
     8041 ccagttatgc cggtgcaatt ctgaaagatg agttaaaaca ggcgggtatc acctggagcg
     8101 gaacactgct gcgccagact caggttaacg aacctggaac ggtagttgcc agtaaacagt
     8161 cggccccgct gcacgatctg cttaagatta tgctgaaaaa gtcggataac atgatcgccg
     8221 atacggtttt ccgcatgata ggccatgcgc gcttcaatgt acctggtaca tggcgggccg
     8281 ggtcggacgc tgtgcgtcag atcctgcgcc agcaagccgg tgtcgatatt ggaaacacca
     8341 ttattgccga tggttcaggg ctttcgcggc ataacctgat tgcccccgcc accatgatgc
     8401 aggtgctgca atacattgcc caacacgaca atgaacttaa ctttatctcc atgctgccgc
     8461 tggcgggcta tgacggctct ttgcagtacc gtgcaggtct gcatcaggcg ggcgtggatg
     8521 gaaaagtctc agcgaaaacc ggttcgttgc agggggtata taacctggcg ggattcatta
     8581 ccacagcgag cgggcaacga atggcgtttg tgcaatatct ttctggctat gcagtagaac
     8641 ctgcggatca gcgtaatcgc cgtattccgt tagtgcgttt tgaaagccgt ttgtataaag
     8701 atatttatca gaacaattag tcaaaaagaa accccggcac atggccgggg cttcagatta
     8761 ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc ggcctacaaa
     8821 atcgtgcaaa ttcaacatat tgcaattctc ttgtaggcct gataagcgta gcgcatcagg
     8881 ctgatttggc gtttatcatc agtgattaac gcttgtaaat gaactcaacg ccttcttcgt
     8941 cgtcttcgtc ccagtcgtca tcccagtctt catcatcctc ttcagcaatc tcttcaagct
     9001 gctggcgatg ataatcgtcc cacatgaatt cgactttctc tggctgtttc gcttcttcag
     9061 cctgcacgac cggtttttca aggataaagg tcatcacatc ccagcagaga tctttcacgc
     9121 ccagtccgct cgccgcagag atcagataat atttatcttc ccagcccagc gcttcagcaa
     9181 tcgctttcgc tttctcttca gcttctgcct tatccagcag atcgatctta ttgaacacta
     9241 accaacgcgg tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac
     9301 gcgcgttttc aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga
     9361 cgcggcaacg ttccaggtgc ttcaggaagc ggatacccag acctgcgcct tccgcagcgc
     9421 cttcaatcag tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca
     9481 cacccagact tggcaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg
     9541 ataccgcgcg aataaaggtc gatttacccg cgtttggcat ccccaacata ccgacgtcag
     9601 ccagcagcat cagctccagc agcagctcac gcttatcacc cggcgtaccg ttggttttct
     9661 gccgtggtgt acggttaacg gacgatttga aacgggtatt gcccagaccg tgccagccgc
     9721 ccttagcaac cagcagacgc tgaccgtgtt tagtcatatc gcccatggtt tcaccagtac
     9781 cctggtcgat tacacgcgta cctaccggca ctttaatcgt cacgtcttta ccgcgcttac
     9841 cggtacaatc gcggcttgcg ccgttctgac cgcgctctgc acggaaagat ttttcaaaac
     9901 gataatcgat aagcgtgttc aggttttcgt cggcttccat ccatacgtca ccaccatcac
     9961 caccgtcgcc gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc
    10021 aaccattacc gccatcacct gcaacgacca gaatcgatgc ttcatcaaca aacttcattt
    10081 tattctccat aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta
    10141 atccgcccca aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg
    10201 caccgagata acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg
    10261 ataaaagatc tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct
    10321 gcgctgcctg ccagcgagcc atcgcttccg ccagggcgcc atatcctacc aatgtattca
    10381 gtccgcaaaa aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggct
    10441 ttgccagagg gaagagcgca attgtacata aagtgtacag taaaaacagg atctgcggtg
    10501 aggccagccg acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgccg
    10561 caccgacccc aaagataact ccccaggtgt aatcggtgag ctttgtaaat atctcgacca
    10621 gactggtgtt aaaaaacatc accaggccgc tcaggagcat caatgcccct acaacctgag
    10681 tgctgcgcat tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga
    10741 gttgcccaat cacctgcgaa gcggtcggac tcaggtattg caaggatgag ctgaacagga
    10801 tgaagttccc aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac
    10861 gaaacacgcg taatggcggc aacctcttct tcaccgcaag aatggcacca aggccaatac
    10921 tcgccatcaa gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct
    10981 tcattgcgat tggcaacgcc ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa
    11041 tgcctgcctg ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca
    11101 ccatgcgcat ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt
    11161 catccgttac cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt
    11221 tcgggccttt aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac
    11281 gaccgcaacc tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc
    11341 tacccgccag aacggattcg ccaccgaaac gcttaacgcc caggcgttta gcttctgaat
    11401 cgcgaccgtt acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag
    11461 gtcttaggcg ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg
    11521 cttacgatag tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg
    11581 agcaacaact tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc
    11641 ttcaccgttt gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc
    11701 cagcttttcc aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg
    11761 gaaaaccgcg tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc
    11821 gctataaata ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc
    11881 tacagtcaat acacgaagaa aaaaaacaca acttgtacgg taacgtttat ctgtgccatt
    11941 ttttcagtac aatcacccta tattcctaac cataaaccct aagttgcctt tgttcacagt
    12001 aaggtaatcg gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat caatgagtta
    12061 accgcgcaag atatggcggg tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc
    12121 caactgatca atcagttagg ctattacatc gtcagcggcg gcggtaaacg tattcgtccg
    12181 atgattgctg tactggctgc acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt
    12241 gctgccctga tcgagtttat ccacacggcg actctgctac acgacgacgt tgtggatgaa
    12301 tcagatatgc gcaggggtaa agctaccgcc aacgccgcat ttggcaatgc cgccagcgtg
    12361 ctggtaggcg attttattta tacccgcgcg ttccagatga tgaccagcct cggttcactc
    12421 aaagtgctgg aagtcatgtc agaagctgta aacgtcatcg cagaaggtga agttctgcaa
    12481 ctgatgaacg ttaacgatcc ggacatcact gaagaaaact acatgcgcgt tatctatagc
    12541 aaaaccgcgc gtctgtttga ggctgccgcg cagtgttccg ggattctggc tggctgtacg
    12601 ccggaggagg agaaaggcct gcaggattat gggcgctatc tcggcactgc tttccagttg
    12661 atcgacgatt tactcgatta caatgccgat ggcgaacagt taggtaaaaa tgtcggcgac
    12721 gatctgaacg aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca tggcacacca
    12781 gaacaggcac agatgatccg taccgccatc gaacagggta acggtcgcca tcttctggaa
    12841 ccggttctgg aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca gcgtgccgag
    12901 gaagaagcag acaaagccat cgcagcatta caggtgctcc cggacacccc ttggcgagaa
    12961 gcactcatcg gcctcgcgca catcgctgtt caacgcgatc gttaatcccc tcccttcatc
    13021 ccgcgcagtg cgcgggatga ttccagtaaa ttccataaaa acactaattc agcgctataa
    13081 cacctgaaaa aaagggcgat gaatctttat atattctgaa tattcacact ctttacagga
    13141 actttttaga gcaataggcc atcaggagta tagtgatgcc cgacagaaga agtgttctga
    13201 atgaaagcga ctaacttaag gagtgaggaa agatggaaag taatttcatt gactggcatc
    13261 ccgctgacat cattgcgggt ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca
    13321 gaaatggttt gagttcctca acgctggcaa acgcattatc gcgcccatgg ccgaaaggag
    13381 agatgattat tgcgaaagcc ctgggaactg acccctgggt tatctggcca tcacgctacc
    13441 atgatccgca gacccatgag tttatcgaca gaacgcagtt gatgcgtagc tacactaaac
    13501 cgaaaaaatg agtggtctgg cggtagcccc gcgaacgggg ctgccagctc tcagacgatt
    13561 actcgccttt cacacgctca atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt
    13621 cgtagccacg atcgatgtga taaatacgat caaccaccgt cgtcccttcc gcaatacagc
    13681 cagccagcac caggcttgct gatgcacgca gatcggttgc cataacctgt gcgccagaaa
    13741 gtttttcaac accgtgacaa ataacggtat tgctttcgat ttcggcatgc gcgcccatac
    13801 ggctaagctc cggcacatgc ataaagcggt tttcaaagac cgtttcggtg atgaatccgg
    13861 tcccttctgc caccaggttc aacagcgtga actgggcctg catatcggtc gggaatgccg
    13921 gatgcggcgc ggtacgtacg ttaacagcct tcggacgttt gccatgcata tccaggctaa
    13981 tccagtcttc gccgacttcg atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt
    14041 cgagagtatc tggctgcgcg ttacggcaga taattttgcc gcgagaaatc gccgccgcca
    14101 ccaggaaagt accggtttcg atacgatccg gtagcacgcg atagacaccg ccgcctaaac
    14161 gttccacacc ttcgatgacg atacgatcgg tgccctgacc gctaattttc gcacccagcg
    14221 taatcaggaa gttcgcggta tcgacgattt ccggttcacg cgctgcgttt tcaataatcg
    14281 tggtaccttc cgccagggtt gcagcacaca tgatggtcac cgttgcgcca acgctgactt
    14341 tatccatcac gatatgtgcg cctttcagac gaccatcgac ggaagcttta acgtaacctt
    14401 cttccagttt gatggtcgcg cctaattgtt cgaggccaga aatgtgtaga tcaaccggac
    14461 gcgcaccgat cgtacaaccg ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca
    14521 gcggccccag tgcccagata gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga
    14581 atacattaac gtcgcgggca tcaatatgca cagaaccatt acgttctact ttcgcaccca
    14641 gctggcttag cagcttcatt gatgtatcga cgtctttcag ttttgggacg ttctggatct
    14701 ctaccggttc ttccgccagt agagcggcaa aaaggatagg cagagcagca tttttagcgc
    14761 cggaaattgt gacttcgccc tggagcttcg ttggcccctg aacacgaaat ttatccattt
    14821 agtttgttct cagttaacaa ttcatatccg ctaccggcga atcgcccata gctcaaaagc
    14881 cgttcagttt gcgatcgcgc gcccactccg caggggtata cgctttgatc gacacagcat
    14941 gaatgcggtt atccgcaata tattccatca gcgggccata gaccgtctgc tgttttttaa
    15001 cccgactcat gccgtcaaac aactcaccca cggcaataac ctgaaagtgg ctgccatcgc
    15061 cggaaacgtg gacttcctgg agggagagag cgttcatcaa cacgctctga atttcattat
    15121 tttccatggg atcttcaatc atcagttaat aaaccagcga aacatcttag agcaaagttg
    15181 cgctggcata aataagcaaa aagcctcgct gataaatcag acaaggctcg acttgcaggc
    15241 aggtttgccg gacaggcggt taacgccata tccggcctga aaaaatttaa cgaggcagaa
    15301 catcagcagg caaattatac aatttcgcga gggtatacac tttgtcgttt accccctgaa
    15361 gcgtcacgtt gttgccctgc tttttcgcca gatcgataag atggagcagc agtgccagtc
    15421 cacccgtatc cacgcgggac acacggctaa gatcgatgca ggtaatcccc ttcaccgctt
    15481 cctcacgcat ttcccaaagc ggtagcagaa cgtcctgatc cagctctccg gataacgcca
    15541 gcgtgtcacc cgtctgcatc cagctcagtg actcgctcat tattttttct cttccagagt
    15601 gattttctgt tgagaaatcg atttcagttg cgcagtcagg ccgtcgatac ctttggtacg
    15661 cagcagcgtt ccccactcgt tttgtttggt ggtgatcata ctgacgcctt cagcaatcat
    15721 gtcgtaagcc tgccagtttc ccgtctggga gtttttacgc cactggaagt ccagacgcac
    15781 cggcggacgg ccattcgggt caataatggt aacgcgaata ggcacaatgg ttttatcgcc
    15841 cagcggctgt tctggcgcaa tctgataggt ttgaccgtga tacatcgcca gcgcctgacc
    15901 gtaagcctgc ttcaggtact cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt
    15961 cgcactcttg taatactggc ccagcaccag cgcaccggcg tatttcacct gtacgtatgg
    16021 cagcagttcc tgatcaacaa tggtacgcag ataatccggg ttggcccgaa tttgcggttg
    16081 ctcattcttc aggcgatcga acgttttctg cgccgcctcg tccatcagct tataaggatt
    16141 ggtctggtct gcctcggttg ccgcactcag aggtgcaatc accagcaaag cgaccatcat
    16201 taaacgttta aacatgcgtc ggttctcctg aaattatttc gttgtaccca caggttcagt
    16261 ggtttcatta ttacctggcg cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc
    16321 tttactaccg taaaggaact gaccaatgag atcttccagc accatcgcag acttagtgtc
    16381 ctgaattgta tcgccatcct tcaggatagc agtccccagt tccgggtctt caaaaccgac
    16441 gtttaatgcc agatattgtt cccccagcag gccggaagta cgaatgctca gcgaactggt
    16501 atctggaatg tggttataac gttgttcaat ttccagcgtt acgcgcggca gataggtttt
    16561 cgggtccagc gtaatatccg ccacccgacc cacaacaacg ccaccaatac tgaccggaga
    16621 gcgcgctttc aggccgccaa tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt
    16681 acgtatggac gtcacgttcg ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc
    16741 taataaaaag atacccaccc aaatttcatt ttttttcgtt tgcatgaact caattcccaa
    16801 acatcaatgc ggtcagcaca aaatccagcc ccagaacagc cagagacgag tggacaacgg
    16861 tgcgagtggt tgcccggcta atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca
    16921 acgaaatcca cgtcaccgtg atggcgaaca ccacgctctt aatcagacag ttgaccagat
    16981 ccatacgcca gtcgacggca ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt
    17041 tccaactgac gccgaccaga gatccgcccc agatccccac ggcgacgaaa ataaccgtca
    17101 acagtggtaa tgaaataacc ccagcccaga aacggggaga aataacccga cgcagcggat
    17161 ccaccgccat catctccata ctggagagtt gctctgtagc gcgcatcagg ccgatttctg
    17221 cggttagcgc cgaaccagca cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt
    17281 cacgcagtag cgataacgcc accagcatac ccagactggt ttccgcacta taagtggtca
    17341 gaaccagata accttgcagc cccaacacca ttccgatgaa cacgccagaa accacaataa
    17401 tcagcatcga caggacgccg acattataga gctggcgcac cagcagcggc gcatgtttgc
    17461 gaaattccgg tttgccgacc agcgcattga ataacattaa cccggcccgc ccgaacgttc
    17521 tcagggtttt aatcccttta tgtccgagcg acgccagcgc atttaacagc atgagtggct
    17581 taactccctg gtaaaagatc agcgtgataa tcgccggcag gatagcggaa cggaacaggc
    17641 ccgtcagcta tcccgtccag aaactgacgt acgcgcggat caggattcgc ctgcaacgcc
    17701 tgggcactgc catgagcgac aatttttttg tccgccagga tccaggcgtg atccgcaata
    17761 cttaacactt ccggcacatc gtgagaaacc accacacaag tcacgcccag cgcgctgttc
    17821 agctcagaaa tcagcttcac cagtacgccc atggtgatgg gatcttgccc aacaaaaggt
    17881 tcatcaaaca tgatgagatc cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc
    17941 gccatcccac cggaaagttc agaaggcatt agtttagccg ctccacgcag ccccacggcc
    18001 tccagcttca tcatcaccgt actatgcaac aatggcgcgg gtagttgggt atgttcgcgc
    18061 agtggatagg cgacgttgtc aaatacgttc atatcagtga acaacgcccc ggactgaaat
    18121 aacatgctca tccgtttgcg cactgtatac aggcgcgaac gagacatcgc cggaatattc
    18181 tcaccatcga aaaggatctc accatgatct ggtgcgattt gcccgccaat cagacggagt
    18241 agcgtcgttt taccgatgcc cgatggcccc atgatcgccg tgatcttccc tcgcggcacg
    18301 gtcagggaaa tattatcgaa gatgcagcga ttgccacgcg taaaactgac atcgcgcata
    18361 tcgactaaat tcgccacaga ctgctccata attcaccctt cgtcttgcgt tgattttcta
    18421 agcatggcgc tcaatttaac cttgaaccca acatatttac agaatattac ccgccgtggt
    18481 tagcgaaagc tggcatttgt tttacttttt agccgcataa agtcaaaatt aagaatccgt
    18541 tacggctttc tgaaaatctt cagcggaccg acgagtatac ctgaagaaag gacgttagat
    18601 gcttttagct acggcactgt taattgttgg tttacttttg gtcgtttaca gtgccgaccg
    18661 cctggttttt gccgcgtcta ttctttgccg aacctttggc atcccgccgc tgatcatcgg
    18721 catgacggtg gtcagtattg gtacatcgtt accagaaatc atcgtctcgc ttgccgcgtc
    18781 tctgcacgaa caacgcgatt tagccgttgg tacagccctc ggctcaaaca ttatcaatat
    18841 tttgctgatc ctcggtctgg ctgcgctggt tcgtcctttt accgtccatt ccgatgttct
    18901 gcgtcgtgaa ttacccttaa tgttgttggt cagcgtagtg gccggttccg tactctatga
    18961 cggacaactt agtcgcagcg atggtatctt tctcctcttt ctggctgtgc tatggctgct
    19021 gttcattgtt aaacttgcac gtcaggctga acgtcagggg actgacagcc tgaccagaga
    19081 gcagcttgca gagctgccgc gtgacggcgg attgcccgtc gcgtttttat ggctcggcat
    19141 tgcgcttatc atcatgccag tggccacgcg gatggtggtt gataacgcca cggtgctggc
    19201 gaattacttt gccatcagcg agttgacgat gggtctgacg gcaattgcta tcggaaccag
    19261 cctgccggaa ctggcaaccg caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt
    19321 cggaaatatc attggcgcaa acatttttaa tattgtcatc gtgttgggtt tacccgcgct
    19381 gataacgcca ggagagattg agccattggc gtacagtcgt gactacagcg tgatgttgct
    19441 ggtgagcatt atttttgcgt tgctgtgctg gcggcgctcc ccgcaaccgg gccgtggtgt
    19501 aggggtatta ttaactggcg gatttatcgt atggctggcg atgttgtact ggttatcgcc
    19561 aatactcgtt gaataactgg aaacgcatta tgtcgcacgt agagttacaa ccgggttttg
    19621 actttcagca agcaggtaaa gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg
    19681 atcaatacat caatcagaat ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga
    19741 aagttgtcgt catggggatg gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt
    19801 ttgccagcac cggtacacct tcatttttcg tccatcctgg tgaagccgcg catggtgatt
    19861 taggcatggt caccccacag gatgtggtga ttgctatctc taactcaggt gaatccagcg
    19921 aaatcacggc cttaattcca gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg
    19981 gtcgcccgga gagcagcatg gcgcgcgccg cagatgtgca tctgtgtgtt aaagtagcga
    20041 aagaagcctg tccgttaggg ctggcaccga ccagcagcac caccgccacg ctggttatgg
    20101 gcgatgccct cgctgtcgcg ctgttaaaag cacgcggctt tactgctgaa gattttgcgc
    20161 tctcacaccc aggcggcgca ctgggtcgta aacttctgct gcgcgtaaac gatattatgc
    20221 atacgggcga tgagatcccg catgttaaga aaacggccag tctgcgtgac gcgttgctgg
    20281 aagttacccg caaaaatctt ggtatgactg tcatttgcga tgacaatatg atgattgaag
    20341 gcatctttac cgacggtgat ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt
    20401 taagtattgc cgatgtgatg acgccggggg gaatacgtgt gcgccctggc attctggccg
    20461 ttgaggcact gaacttaatg cagtcccgcc atatcacctc cgtgatggtt gccgatggcg
    20521 accatttact cggagtgtta catatgcatg atttactgcg tgcaggcgta gtgtaaagat
    20581 tcaaggataa acaacaatga gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt
    20641 cagcgccgac gttatagcaa aagcagagaa cattcgtctg ctgatcctcg atgtcgatgg
    20701 cgtactgtca gatggcctga tttatatggg caataatggc gaagagctga aagcgttcaa
    20761 tgttcgtgac ggttatggca ttcgttgtgc gctcacctct gatattgaag tcgctatcat
    20821 taccgggcga aaggctaaac tggtagaaga tcgttgtgcc acattgggga tcactcactt
    20881 gtatcagggg cagtcaaaca aactgatcgc ctttagcgat ctgctggaaa aactggcgat
    20941 tgccccggaa aatgtggctt atgtcggcga tgatctcatc gactggccgg taatggaaaa
    21001 agtgggttta agcgtcgccg tggccgatgc gcatccactg ttgatcccgc gcgccgatta
    21061 cgtgacgcgc attgctggcg gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct
    21121 ggcgcagggc aaactggatg aagccaaagg gcaatcgata tgagtaaagc cagacgttgg
    21181 gttatcattg tgctatcact ggcggttctg gtgatgatcg gcattgatat ggccgaaaaa
    21241 gacgataccg cccaggtggt cgtcaacaac aatgatccca cctataaaag cgagcatacg
    21301 gacacgctcg tctataaccc agaaggggca ctaagctatc gattgattgc tcaacacgtt
    21361 gaatattatt ccgatcaggc cgtttcgtgg tttacgcagc cggtacttac cacgtttgat
    21421 aaggataaaa tcccgacatg gtccgtaaaa gcagataaag ccaagctgac caatgaccgg
    21481 atgctctatt tatatggaca cgttgaagtc aacgcacttg tgccagactc tcaacttcgc
    21541 agaatcacga cggataacgc gcagatcaat ctggtgacgc aggatgttac ctctgaagac
    21601 ctcgtcacgt tatacggaac aacatttaac tccagcggtc tgaaaatgcg cggcaactta
    21661 cgcagcaaga acgccgagct gattgaaaag gttagaacat cctatgaaat tcaaaacaaa
    21721 caaactcagc cttaatcttg tgcttgccag ctcacttctg gccgccagca ttccggcatt
    21781 tgccgtaacc ggagacactg atcagccgat ccacattgaa tcggaccagc aatctcttga
    21841 tatgcaaggc aacgtggtta cctttaccgg taatgtcatc gtcacccagg gcaccatcaa
    21901 aattaatgcc gacaaagtgg tcgttacccg tccgggcggc gaacaaggta aagaagtgat
    21961 tgacggctac ggtaaaccgg caacgttcta ccagatgcag gacaacggta aacccgttga
    22021 aggtcacgct tcccagatgc actacgaact ggcaaaagat tttgtcgttc tgacgggtaa
    22081 tgcttatctg cagcaggtcg atagcaacat taagggcgat aagatcactt acctggtaaa
    22141 agagcagaaa atgcaggctt tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc
    22201 gtcgcagctg caggacaaaa acaacaaagg ccagaccccg gcacagaaga agggtaatta
    22261 attcgttatg gcaacattaa ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt
    22321 ggtagaagac gtcagcctga ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa
    22381 cggtgccggt aagaccacca ctttctacat ggttgtaggc attgtgccgc gcgatgcggg
    22441 caacatcatt attgatgatg acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg
    22501 cggtatcggc tatctgccac aggaagcctc cattttccgt cgcctcagcg tttacgataa
    22561 cctgatggcg gtactgcaaa ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc
    22621 gaacgagctg atggaagagt ttcacattga gcacctgcgt gacagcatgg ggcagtcact
    22681 ctccgggggt gaacgtcgcc gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt
    22741 tattctgctc gacgaaccgt ttgccggggt tgacccgatc tcggttatcg acattaaacg
    22801 catcattgag cacctgcgcg acagcggcct gggcgtgctg atcactgacc acaacgtgcg
    22861 tgaaacactg gcggtttgtg aacgcgctta tatcgtcagt caggggcatt tgatcgccca
    22921 cggcacgcct acagaaatct tacaagacga acacgttaag cgtgtatacc ttggggaaga
    22981 cttcagactc tgatagggta gaagtttgcg acgttttagc aggagagtac gattctgaac
    23041 atgaagcaag gtttgcaact caggcttagc caacaactgg cgatgacgcc acagctccaa
    23101 caggcaattc gtctgttgca gttgtcgacg ctggaacttc agcaggagct acagcaggcg
    23161 ctggagagta atccgctgct tgagcaaatc gacactcatg aagaaatcga cacccgcgaa
    23221 acgcaagaca gtgaaacgct ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa
    23281 gagctgccgc tcgatgccag ttgggacacc atttacaccg ctggtacacc atccggcacc
    23341 agcggtgact acattgacga tgagctgccg gtctatcagg gcgaaacgac gcagaccttg
    23401 caggattacc tgatgtggca ggtcgagctg acaccgtttt ccgacactga ccgcgctatt
    23461 gctacctcta tcgtcgatgc cgttgatgac atcggttatc tgactgtccc gctggaagat
    23521 attctcgaaa gtatgggcga tgaagagatc gacatcgacg aggttgaagc cgtccttaag
    23581 cggatccaac ggtttgatcc ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta
    23641 atccaactct cccaattcga taaaaccacg ccgtggctgg aagaggccag actgatcatt
    23701 agcgatcatc tcgatctgtt agccaatcac gacttccgca ctttaatgcg cgtcacgcgt
    23761 ctgaaagaag atgtgctgaa agaagccgtc aatctgatcc agtcgctcga tccgcgcccc
    23821 gggcagtcga tccagactgg cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag
    23881 cataacggtc actggacggt agaactcaac agtgacagca ttccgcgtct gcaaatcaac
    23941 cagcactacg cctcgatgtg caataacgcg cgcaacgatg gtgacagcca gtttatccgc
    24001 agcaatctgc aggatgccaa atggttgatc aagagtctgg aaagccgtaa cgatacgcta
    24061 ctgcgcgtga gtcgctgtat cgttgaacag cagcaagcct tctttgagca aggtgaagaa
    24121 tatatgaaac cgatggtact ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg
    24181 atatctcgcg tgaccacgca aaaatacctg catagtccac gaggcatttt tgaactgaag
    24241 tatttctttt ccagtcacat caataccgag ggcggcggcg aagcttcctc cacggcgatt
    24301 cgtgcgctgg tgaagaaatt aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc
    24361 aagttaacct ctttgctgtc ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag
    24421 taccgagagt ctttatccat tccgccgtca aaccagcgta aacagctcgt ttgacccaac
    24481 cgataaggaa gacactatgc agctcaacat taccggaaat aacgtcgaga tcaccgaggc
    24541 actgcgcgaa tttgttacag ccaaatttgc caaacttgag caatattttg accgaatcaa
    24601 ccaggtctat gttgttctga aagtggagaa agtcacccac acctcagatg caacactgca
    24661 tgtaaacggt ggtgaaattc atgccagcgc ggaaggtcag gacatgtacg ctgccattga
    24721 tggcttaatt gataagctgg cacgccagtt gaccaaacat aaagataaac tgaaacaaca
    24781 ctaattgtcc gggcaattag catgtgcatg gcggcctgtt gtgcggcaca acgggccatt
    24841 tgtacggtta atgctccgag cctgttccac tgtttgagtg ggcaggttct taggtgaaat
    24901 tatgacaaat aatgatacaa ctctacagct tagcagtgtt cttaacaggg aatgtacgcg
    24961 aagccgcgtc cactgtcaga gcaaaaaacg cgccctggaa atcatcagcg agctggcggc
    25021 gaaacaactt agcctgccgc ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat
    25081 gggcagtacc ggtatcggca atggtattgc cattccgcat ggcaaactgg aagaagatac
    25141 tctgcgcgcc gttggcgttt tcgttcagct cgaaacgcct atagctttcg atgccatcga
    25201 caaccaaccg gtggacttac tttttgccct gctggtgtcg gcagaccaaa ctaaaacgca
    25261 cctgcatact ctgtcgctgg tggcgaaacg tctggcggac aaaaccatct gccgccgttt
    25321 acgcgcagcc cagagcgatg aagagctgta tcaaatcatt acggataccg aaggtactcc
    25381 ggatgaagcg tagttattcg gtaatgtctc ttttagacgt tgtgaggaga aacagtacat
    25441 ggtactgatg atcgtcagcg gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct
    25501 ggaagatatg ggtttttact gcgtggataa ccttcccgta gtgttgttac ccgatctggc
    25561 tcgaaccctg gccgatcgcg agatttctgc cgccgtcagc attgatgttc gtaatatgcc
    25621 ggagtcacca gaaatattcg aacaggcgat gagtaacctg cctgacgctt tctcaccgca
    25681 actcctgttc ctggatgccg accgtaatac cttaattcgt cgttacagtg acacgcgccg
    25741 actgcatccg ctttccagca aaaacctgtc gctggaaagt gctatcgaca aagaaagcga
    25801 tttgctggag cctctgcgtt cgcgagcgga tctgattgtc gatacctcag aaatgtccgt
    25861 tcacgagctg gctgaaatgc tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac
    25921 catggtcttt gagtctttcg gcttcaaaca cggtatccct atcgatgcag attacgtctt
    25981 tgacgtgcgc ttcttgccga acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct
    26041 tgataaacct gtcgccgcgt tcctcgaccg ccacacagaa gtacacaatt ttatctacca
    26101 gacgcgaagc tatcttgagc tatggttacc tatgctggaa accaacaacc gtagctactt
    26161 gacggtcgcc attggttgta ccggcgggaa gcaccgttcg gtgtatattg cagagcaact
    26221 ggcagactac ttccgctcgc gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa
    26281 acgtaaacca tgaccgtcaa gcaaactgtt gaaatcacaa acaagctggg catgcatgcc
    26341 cggcctgcaa tgaagctgtt tgaattaatg cagggttttg acgctgaagt gctgttacgt
    26401 aatgacgaag gcaccgaggc cgaagccaac agcgttattg cgctgctgat gttggattct
    26461 gccaaaggac ggcagattga agttgaagcg accggcccac aggaagagga agcactggcc
    26521 gccgttatcg ccctctttaa ctctggtttt gatgaagatt aatcttcatc actttgacat
    26581 acaactccct tcaaactccc cccgacaata agaaaatcac gtactgaaat cgttctcaat
    26641 caacgtcatt tgtacatttt gtgcgctttt cactttcaga agaaccttaa gaaaacctta
    26701 agaggcattg tttaggtttt gtttaagtta atcgaccata ctggagatcg tcagaaaata
    26761 tttccaggag atggcatgat tcgcttatct gaacaaagtc ccctgggcac cggaagacat
    26821 cggaaatgtt atgcgcatcc ggaagatgcc caacgctgta tcaagattgt ctaccatcgt
    26881 ggcgatggtg gcgataaaga gatccgccgt gagttaaagt actacgcgca tcttggtcgc
    26941 cggttaaaag actggagtgg aataccgcgc tatcacggta ccgttgagac ggattgcgga
    27001 accggttatg tctacgatgt gattgcagat tttgacggca aactctccat tacgctgacc
    27061 gaatttgctg aacaatgccg ttacgaagaa gacattgcac aactgcgcca gctactgaaa
    27121 cagctgaagc gttatttgca ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat
    27181 atcctctgtc accgcatcag tgagtcagag gttatcccgg tcgtctgcga caacattggt
    27241 gaaagcacat tgattccact ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag
    27301 cggttatgga aacgatttat cgcccaaccg gcgctggcta ttgccctgca gaaagatctg
    27361 caacctcgtg agagcaaaac gctggccctc acttcgcgcg aagcttaatc cagctggtgt
    27421 tgttgcataa acggctcacc gcctaactga tacatctgcc gtaaaatcca cgcctgacgg
    27481 ctacgcacgt agcccgatgg cgaggagact ttgaaacgaa gtggattagg taatacagct
    27541 gccagtaatg cagcttccga ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt
    27601 gccgcagctt cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc
    27661 aggatacgct ttttgctcca gactgtttct atccccagcg ttaatccagc ttccagccct
    27721 tttcgtaccc agctacgccc atcccataaa aagagatttt tggccgtctg ctgagaaatc
    27781 gttgaagcac cgcgaatacg gttttcattg cgctcgttgt gcgccagggc tttctcaatg
    27841 gaagcgacat caaagcccca gtgctcagga aatttctgat cttctgcggc aatcaccgcc
    27901 agtcccatcc acggcgagat ttgatccata ctgacccagt cagaatgtgc cacgtaacga
    27961 aaattgccat gcagccaggc gctgacctgt cgctcgacca ttaccgctga gaagggaaca
    28021 ggcgcaacac taaacaacgc gatgcccccg ccccagaaaa cggcgaggac aaccattaac
    28081 agcaaaagaa aacggcgaac gaaactaaac accgttaagc ggcttttact cattcagcca
    28141 gaaccagcac gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca atgttctgcg
    28201 ccagcatata tgctggggtg gtgacaatct tattgtcttc atcaaccacg atatcatcga
    28261 caggacaggg cacatgctcc gcgcccatct cttccagcac ttctgcggta tcgatatcag
    28321 taccgatggt caaacgcagc gggaaatcga aaattttcgg cagcatcgcc ggggcaatac
    28381 acataaaacc aagcggtttt ccggcctgat gcatcgcttg tgccagcgcc tttaattcac
    28441 ggtcaacggt gcattcgcta ccaagagtgg caaaattgct taaattcttc gccgcgccaa
    28501 acccccccgg cacaatcaac gcatccagtt cagcggcatc ggcctgggcc agaggacgga
    28561 tttcaccgcg cgttattcgt gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt
    28621 cgccagttaa atggttgata acatcaacct gctgcttatc cggtgcaaag cagaccgcct
    28681 gcgcaccgct gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt
    28741 catagacgcc gcatccgctc agaattacgc caattttctt cattgtgatc atcctttcgc
    28801 aattgactga aacacattaa tttttttaat aaaaatggta cgcatcacac atttaactga
    28861 ttcatgtaac aaatcattta agttttgcta tcttaactgc gtgcggcctg aaaaacagtg
    28921 ctgtgccctt gtaactcatc ataataattt acggcgcagc caagatttcc ctggtgttgg
    28981 cgcagtattc gcgcaccccg gtctagccgg ggtcattttt tagtggcttt tgccacccac
    29041 gctttcagca cttctacgtc gtgacgccac tcttctttca tctcttcaat ccattcaccg
    29101 acgttatctt cccaggcagg aaggtcagga gactgaattt gctgacccag ctgttgcaga
    29161 tggcgtaacc ccactgaccc cgccgcacct ttaattttat gtccttcctc aacaatgcct
    29221 tttttatcct gcgccgtcag attcgactcc agcacgctga catagcccgg catcatcttc
    29281 tcaaacaccg ctaacccgtc ggtgatcagc ttcggtccta caagttcgag atactgttcc
    29341 agcatgggaa tatcgagcaa tgcttctgat ttactgttct cttctgtcgt caccgtactc
    29401 tcctcatcat cctgggtatc ccagaatttc ttgatcatcg cggttagcgc cggaacagaa
    29461 agcggcttac tcagcacatc atccattcca gcattgaggt actcttgttt gtctttcagc
    29521 acgttagcgg ttaaggccac cagcggcggt aaatcctctc gcggataacg cttcgtcagt
    29581 tcacgagaga tatccagccc ggtcatatct ggcaactgaa tatccagtaa caccaggtcg
    29641 tattcgcccg gtttaaacat ctctagcgcc gccttgccgg tcatggcgac atcaacgctg
    29701 ttacctaatt tttccagcac agaacgcgca acaatcacgt tcagttcaat gtcttccacc
    29761 agcagcacat tcagcgccgg taaaggcata tcgtcttcat caaacgcatc atcgacctct
    29821 tctgctaccg acggtgcgtg gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg
    29881 gtaaccgtaa tatcgccgcc catatttttc gccagacgac gagaaacggc cagaccaata
    29941 ccggtgccgg tggcaggttt accgccatga ctgtctttca cctggtaata catggcgaaa
    30001 attttatcca gctcatcctg cggaatgccg ataccagagt cttccacttc aaaatgcagc
    30061 atatcgcctt catcgtagcg cacgcgcacg gtaacctggc cttgctgggt gaatttgacg
    30121 gcgttactga tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt aatgacctga
    30181 tgcggtaatg gcagcgtcgg ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat
    30241 gcggagagat tttccagatc ggcaaggaag ctggtgaaat caaccggttg attatcaagc
    30301 tggaccttgc gccgttccat cttatccatg tcgataatat cgttaaagat attccccagc
    30361 gtgacggccg aaacatggat ggtcttgaga tatttttcct gctcggcggt gagttcggta
    30421 tccagcagaa tgcggctcag accgacgata ccgttcagcg gtgtacgcaa ttcgtgactg
    30481 atggtggaga taaacgtcgt tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc
    30541 ttacgctcgg taatgtctcg accaaagccc atcaaaccgt gacgtttacc cacgcggtcg
    30601 tagtacggca ctttacggat ttcaaagcag gctttgcgcc cgtccgggta atccagccac
    30661 tgttcatagg tcagtgacac attatgacgg aacacttttt catcggtttc aatgactttt
    30721 gcggcggctt ccggtgagta aacatcagca ggtttcaggt gaaccagttg tttttcgctt
    30781 tttccggtca gcagctccat cgcgcggtta cagccggaaa actctttatc ttcgttacga
    30841 taaaaaacca ggtcgggtga agcatcaagg aaggaacgta agaatgagga ttgctgctcg
    30901 agctgaattt gtgtctcttc gcgctctttg atttcaattt tcagttggcc gaaggtttcc
    30961 tgcagttctg cttccgcttt ttcacgaacg gcgatttcct gatttagctg ggcaatatta
    31021 tcttttaact gaacgttgag gctcaaatcg cgctcgcgca tctcctccag tttttgcacc
    31081 agccgtgaca gacgttgtcg tgactcctcc agttgctcga cgaccaccga tagaaagtag
    31141 accgcccacg gcgtaatcaa caaaccaaag aagatagaac gaataacatc aatgctttcg
    31201 acctgaccat gcagcaccat ggttaccgcc atttgtacca caatggcaag aacgacgagg
    31261 gccagcgcca gcaacattga gaagcgcacc agacctaact tcatcatcag gtcaacataa
    31321 tactgcgcca gcagacgaat ttgcttcatt agggaattcc ttcacgacaa cctgccccaa
    31381 taatacccaa ttctgacagt tacgttataa attgtgtgag aaatgcggaa ttcccctcaa
    31441 cgacctactc cgttggaggt agccacggac gtcccagcgc cgatccctgt acgccatgtt
    31501 cattcagata gcgatccagc tcgaccattc ccgtccatcg attttcgcac cacagaggag
    31561 caagcagcgt cgggcgacgg gcgctggcgg aaatgcggtg gtagatcact tccggtggcg
    31621 tatggcgaat catttctccg gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac
    31681 gccccgcttc ccaggctttc gccataatgc tgcctttcac aatatgcaac ggatgtagct
    31741 ttataccgtc aacaccggtt tccactaccc gctccagcgt ttgcagacat tcagccagcc
    31801 cttcgcctgg cagaccgaca attaaatgcg agcacacctt cagcccgcgc tggcgtgcca
    31861 gctgcgtcgt gcgctgataa caggcgaaat catgaccgcg attaatgcga tgtagcgttt
    31921 tgtcgtgcac ggtttgcagc cccagctcca gccacacttc gtagccctgg tctttatatt
    31981 cgcaaagcag atccagcacc gcatccggca cgcagtccgg gcgcgtgccg acacacaaac
    32041 cgacaatatt ggcctggctc accgcctgtt gatacatcga acgcagcacc tgaacttcgg
    32101 caaaggtgct ggtatacgcc tggaaatagg cgaggtaacg tttcgcgcga ttaattaaat
    32161 tcgcctgatg cgccagctgt tcggcaatgg aacgatgctg ctgcgcttcg tcggcaaacg
    32221 aggcaacatt acagaaggtg cagccgccgc gcccgatggt gccgtcgcga ttcgggcaac
    32281 tgaaaccgcc gtgcagcgtc agcttatgca ccttttgccc ataacgacgg gtgagatcac
    32341 caccaaacat attgactaat ttctgtaact gcataatctg atagaccgcg ccttgaaaag
    32401 aggccaaagc ctgccatttt tagctcaatt cggcgatgac ctggatcaat cgtcccggcc
    32461 cgcttttatc aactgcataa tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa
    32521 tcacttttgc ttatcttgtg tcagattttt ttatctcctt atggatttta ggcaaaaaca
    32581 gtagcatgaa acgtcattac caattaatgc agtataaaat gctggttttc tcgtcaattc
    32641 aaggcaggat aagggttaac acgcctttat gacagtcagg aattgactgt ttctctaacg
    32701 acttcccttt tagccttaaa gataaaatct attttaattt cagccattta ataaagaatt
    32761 ttgcgctaaa gcacatttct gtaccaataa gcttgccatt tgacctgtat cagctttccc
    32821 gataagttgg aaatccgctg gaagctttct ggatgagcag cctgctcatc atatttatgc
    32881 agtaattgag atcccctctt caccgtatta accgatgcga aaaggacaac aagggggcga
    32941 atgcgaggcg cgcgtatgac acgcaacccc cgtcgccacg ctctttctgt acccgtgcgc
    33001 agcggttcgg tagtggggtt cccgcagagc ctgggggagg ttcacgatat gttgtacgat
    33061 aaatcccttg agagggataa ctgtggtttc ggcctgatcg cccacataga aggcgaacct
    33121 agccacaagg tagtgcgtac tgcaatacac gcactggccc gcatgcagca ccgtggcgcg
    33181 attctcgccg atggtaaaac cggtgacggt tgcggcttgc tgttacaaaa accggatcgc
    33241 ttttttcgca tcgttgcgca ggagcgcggc tggcgtttag cgaaaaacta cgccgtcggg
    33301 atgctattcc tgaataaaga tcctgaactc gccgctgccg cacgccgcat cgttgaagaa
    33361 gaactgcaac gcgaaacctt gtcgattgtg ggctggcgtg atgtccccac taacgaaggc
    33421 gtactgggtg aaatcgccct ctcctctctg ccccgcattg agcaaatttt tgtgaacgcc
    33481 ccggcaggct ggcgtccgcg cgatatggag cgccgtctgt ttatcgcccg ccgccgcatt
    33541 gaaaagcgtc tcgaagccga caaagacttc tacgtctgta gcctatcgaa tctggtgaac
    33601 atctataaag gtctgtgtat gccggcggat ctgccgcgct tttatctgga tcttgcggac
    33661 ctgcgtctgg aatcggccat ttgcctgttc caccagcgct tctccactaa caccgtaccg
    33721 cgctggccgc tggcgcaacc gttccgctat ctggcgcata acggtgaaat caacaccatc
    33781 actggtaacc gccaatgggc gcgtgcgcgt acctataaat tccagacgcc gcttatccct
    33841 gatctgcacg acgccgcacc gttcgtcaac gaaaccggct ctgactccag ttcgatggat
    33901 aacatgctgg aactcctgct ggcaggcggg atggatatca tccgcgccat gcgtctgtta
    33961 gtgccacccg cctggcagaa caacccggat atggacccgg aactgcgtgc cttctttgac
    34021 tttaactcca tgcatatgga gccgtgggat ggcccggcgg gcatcgtgat gtccgacggt
    34081 cgttttgccg cctgtaacct cgaccgtaac ggtctgcgtc cggcgcgcta cgtcatcacc
    34141 aaagataagc tcatcacctg cgcctctgaa gtcggtatct gggattacca gcctgacgaa
    34201 gtggtcgaaa aaggccgcgt cgggccaggc gaactgatgg ttatcgacac ccgcagtggg
    34261 catattctgc actcggcaga aaccgatgac gatctgaaaa gccgccatcc atataaagag
    34321 tggatggaga aaaacgtccg ccgactggta ccgtttgaag atctgcccga tgaagaagtg
    34381 ggtagccgcg aactggacga cgacacgctt gccagctacc agaaacagtt taactacagc
    34441 gcggaagagc tggactccgt aattcgcgta ctgggcgaaa acggtcagga agcggtcggt
    34501 tcgatgggcg atgatacccc attcgccgtg ctctccagtc agccgcgcat tatttacgac
    34561 tacttccgcc agcagtttgc ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg
    34621 catgttatgt cgctcgccac cagtatcggt cgtgaaatga acgtcttttg cgaagcagag
    34681 ggccaggcgc accgtttaag ctttaaatcg ccgattctgc tctactccga tttcaaacag
    34741 ctcacgacga tgaaagagga gcactaccgc gcagatacgc tggatatcac ctttgacgtc
    34801 actaaaacca cgctcgaagc gacagtcaaa gagctgtgcg acaaagccga aaaaatggta
    34861 cgtagcggca ccgtgctgct ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg
    34921 gttccagccc cgatggcggt tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt
    34981 tgcgatgcca acatcatcgt cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg
    35041 ttgctgggct tcggcgcgac ggctatttat ccataccttg cctatgaaac gctgggccgc
    35101 ctggtagaca cccatgcgat tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac
    35161 ggcatcaaca aaggcttgta caaaatcatg tccaaaatgg gcatctccac catcgcctct
    35221 taccgctgct cgaaactgtt tgaagcggtc ggtctacacg atgatgtagt gggcctgtgc
    35281 ttccaggggg cggtcagccg cattggtggg gcaagctttg aagacttcca gcaggatctg
    35341 ctgaatctgt cgaaacgtgc ctggctggcg cgtaagccta tcagccaggg cggcctgctg
    35401 aaatacgtcc acggcggcga ataccacgcc tacaacccgg acgtggtgcg cacgctgcaa
    35461 caagcggtac aaagcggcga gtacagcgac tatcaggaat acgcgaagct ggttaatgag
    35521 cgtccggcaa ccacgctgcg cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac
    35581 attgctgatg ttgaaccggc aagcgaactg tttaaacgct tcgataccgc tgcgatgtct
    35641 atcggcgcgt taagcccgga agcccacgag gcgctggcgg aagcgatgaa cagcatcggc
    35701 ggtaactcaa actccggtga aggcggtgaa gatccggcgc gctacggcac caacaaagtg
    35761 tcgcgcatca agcaggtggc ttccggtcgc tttggggtta ctccggcgta tctggtcaat
    35821 gccgacgtca ttcagattaa agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg
    35881 ccgggtgata aagtcactcc ttacatcgcc aaactgcgct attcggtgcc cggagtgacg
    35941 ctgatctccc cgccgccgca ccacgatatc tactctatcg aggacttagc gcagctcatt
    36001 ttcgacctca agcaggttaa cccgaaagcg atgatctccg tgaagctggt ttccgaaccg
    36061 ggcgtaggca ccatcgcgac tggcgtggca aaagcttatg ccgatttaat caccatcgca
    36121 ggctatgacg gtggcaccgg cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg
    36181 tgggagctgg ggcttgttga aacccagcag gcgctggttg ccaatggtct gcgtcataag
    36241 atccgtttgc aggtcgatgg cggcctgaaa accggtgttg atatcatcaa agcggcgatt
    36301 ctcggcgcag aaagcttcgg cttcggcact ggcccaatgg tcgcgctcgg ctgtaaatat
    36361 ctgcgtattt gccacctgaa caactgcgca acgggtgtgg caactcagga tgacaaactg
    36421 cgtaagaatc actatcacgg cctgccgttc aaggtgacga attactttga gtttatcgcc
    36481 cgtgaaaccc gcgagctgat ggcacagctg ggggtaacgc ggctggtgga tctgattggt
    36541 cgcaccgacc tgctgaaaga gctggacggt ttcaccgcca aacagcagaa actggcgctg
    36601 tcgaagctgc tggagaccgc cgaaccgcat ccaggtaagg cactctactg caccgaaaac
    36661 aacccgccgt ttgataacgg cctgctgaac gcgcagttgc tgcaacaggc gaaaccgttt
    36721 gtcgatgaac gccagagcaa aaccttctgg ttcgatatcc gcaataccga tcgctccgtg
    36781 ggcgcgtcgc tttcaggcta tatcgcccag acgcacggcg atcaggggct ggcagccgat
    36841 cctatcaaag cgtacttcaa cggcaccgca ggccagagtt tcggtgtatg gaacgcgggc
    36901 ggcgtggaac tgtacctgac cggggatgcc aacgactatg tcggtaaagg catggcgggc
    36961 ggcttaatcg ccattcgtcc tccggttggt tccgccttcc gcagccatga agcaagtatt
    37021 atcggcaaca cctgcctgta tggcgcgacc ggtggtcgtc tgtatgccgc aggccgcgcg
    37081 ggtgaacgtt tcggcgtgcg gaactccggt gctatcaccg tggtagaagg cattggcgac
    37141 aacggctgtg aatatatgac gggcggtatt gtctgtattc tgggtaaaac cggcgttaac
    37201 ttcggtgcgg gcatgaccgg cggcttcgct tacgttctcg atgaaagcgg tgatttccgc
    37261 aaacgcgtta acccggaact ggtcgaggtc ttaagcgttg acgatctggc gatccatgaa
    37321 gagcatctgc gcggtcttat caccgagcat gttcagcata ctggctctca gcgcggtgaa
    37381 gagattctgg cgaactggtc aaccttcgcc actaaatttg cgctggttaa accgaagtcc
    37441 agtgatgtaa aagcactgct gggtcatcgt agtcgtagcg cagcagagtt gcgcgtgcag
    37501 gcgcagtaag gggtagcaac aatgagtcag aatgtttatc aatttatcga cctgcagcgc
    37561 gttgatccgc caaagaaacc gctgaagatc cgcaaaattg agtttgttga aatttacgag
    37621 ccgttttccg aaggccaggc caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca
    37681 tactgcgagt ggaaatgtcc ggtacacaac tacatcccga actggctgaa gctcgccaac
    37741 gaggggcgta tttttgaagc ggcggaactg tcgcaccaga ccaacaccct gccggaagtt
    37801 tgcggacgag tctgcccgca agaccgtctg tgcgaaggtt cctgcactct gaacgatgag
    37861 tttggcgcgg tgaccatcgg caacattgag cgttatatca atgataaagc gttcgaaatg
    37921 ggctggcgtc cggatatgtc cggcgtgaag caaaccggta aaaaagtggc gattatcggc
    37981 gcaggcccgg caggtctggc gtgtgcggat gtcctgacgc gtaacggcgt aaaagccgtt
    38041 gtcttcgacc gtcatccaga aattggcggg ctgctgacct tcggtattcc ggccttcaag
    38101 ctggaaaaag aggtaatgac gcgtcgccgt gaaatcttca ccggcatggg tattgaattc
    38161 aaactcaata ccgaagtggg ccgcgacgtg cagctggacg atctgctgag tgattacgat
    38221 gccgtcttcc ttggcgtcgg gacttatcag tcaatgcgcg gcgggctgga aaacgaagac
    38281 gccgatggcg tgtacgcagc gctgccgttc cttatcgcca acaccaaaca gttaatgggc
    38341 tttggtgaaa cccgcgacga accgttcgtc agcatggaag gcaaacgcgt ggtggtcctt
    38401 ggcggtggcg acactgcgat ggactgcgtg cgtacgtccg tgcgccaggg agcgaagcac
    38461 gttacctgtg cctatcgtcg tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa
    38521 aacgcgcggg aagaaggcgt agagttcaaa ttcaacgtcc agccactggg gattgaagtg
    38581 aacggtaacg gcaaagtcag cggcgtaaaa atggtacgta ccgaaatggg cgaaccggat
    38641 gccaaaggcc gtcgccgcgc ggagatcgtt gcaggttccg aacatatcgt tccggcagat
    38701 gcagtgatca tggcgtttgg tttccgtcca cacaacatgg aatggctggc aaaacacagc
    38761 gtcgagctgg attcacaagg ccgcatcatc gccccggaag gcagcgacaa cgccttccag
    38821 accagcaacc cgaaaatctt tgctggcggc gatatcgtcc gtggttccga tctggtggta
    38881 accgctattg ccgaaggtcg taaggcggca gacggtatta tgaactggct ggaagtttaa
    38941 gctctgtccc agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc ctttctttct
    39001 ggaaagcgcc tcgcataatc tgaagaaaac caatgtaata cgctcatctg gattgttacc
    39061 ggtagatgcc cggcgtatag ctgattccat tattcaacgc agcgccatat aaagggacag
    39121 ataatggaat cgctctcaga gagaacctca acaggctacc agcaaatcca cgacggtatt
    39181 attcacctgg tcgatagcgc ccggacggaa acggtacgta gcgttaacgc gctgatgacc
    39241 gcgacatact gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg cgaggcaagg
    39301 gctgcgtatg gtgcgcagct aatcaagcga ctatcaaagg atttaagtct aaggtataag
    39361 cgtggtttct ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt ttttcaacat
    39421 gttgaaattc gccagacagt gtctggcgaa ttaacaccat tgggaattcc gcagacacca
    39481 tctgcagaat tcccatcggc aaagatttgg cagacgctgt ctgccaaatt gagaggctgt
    39541 ttgatatttc cacagctttt gtattgcatc tcctattttc ggtttgtata ttcatgcaaa
    39601 taaacgaagg aaagttgccg tgatttacat tgaacagctt gaattaatcc ataaaagtgg
    39661 tgatgtgttg tatccagtaa aaataaccag aaaatcatca ggaaaaactg ccttccacct
    39721 tgttccattc ggcttaaata agactcacga ccttctagag gtggaagatg cttcagaagc
    39781 aattcggctg gtgattgatg aacgtcattc cattcgctgc agtacactta cagcaactat
    39841 tacgaataaa aagggcaagc gaattaagcg gacaggtata tacagtatca agggtgttaa
    39901 cataaaagaa tacaacgtca ggtaatggca cagagcggtc cgtcagttca ggtttggctc
    39961 tgtgccaaag atgggtcagg tcacacctga tctaataaat ttaagccatt aaatcagcct
    40021 taaccttcac caccaccttt ttaatctcac ccggctcgcc aacaacgcat cccggcttat
    40081 gcggctctcc tagcataaac acggcgaaca ttcccggttt caggatgatg gcttgctcgt
    40141 tttcaatggt gctgcaaagc tggtaatcat cctcatggtg gaactcttca cactgacgcg
    40201 cagtgcctgc catgccaaac agaatccgtt cttcaccgtt taataacagc tggatatcaa
    40261 tgtattgctc gtgcagctcc gcttttttct caacgggcga ttgagtgtta aacgtcatga
    40321 cattcataaa aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc
    40381 tggcagctaa tgccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta
    40441 atgaccgtac ttcacccatc atcataattt ttctccctgg gccaacagcg ctgccccaag
    40501 taaacctgca tcatggcggt agtgcgccgc cagtaaatca acatgaaata ccgctggctc
    40561 ctgcgccaga tacgtttcca ccaacgccag atacccttct gccagaccaa cgctgccacc
    40621 gaccaccacg cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgccagcac
    40681 atgtgcggag cggtgaatca gttgctgcgc ctgctcgtcg ccctgcccgg cgcgtgtgaa
    40741 aatagttctc gcatccgcgc ccatgagttc accttgcgct gccgctgcaa taccgcgacc
    40801 agaagcaatc gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc
    40861 ggcaagcgta tgcccgatat gccccgccag accgccaggg ccggtaagca gtttaccgcc
    40921 gctcactaca ccgccgccga cgccagtgga aacggtgata aacaccatct cggttacatc
    40981 gccttccagc gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt
    41041 cggcaaattg gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccgag
    41101 attatgcgga ttcagcgcca gcaaactgcc gtcgcgaata attccggttg aagcgatggc
    41161 aacccgctgc gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc
    41221 ttctggtgtc tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc
    41281 gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagtg tggtcatagc
    41341 accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca
    41401 gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg
    41461 ttgtaacgcc cttcggcaat cacccgacat ccggcttcgc tcaacgtttt caccagcgcc
    41521 agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt
    41581 tcggcgccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc
    41641 agtaaaccgt gatgatgtat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg
    41701 tcggtgccgt caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc gacatcttca
    41761 atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc
    41821 ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca
    41881 accgcgcccg cctgctctgc cgctaatgcc atggctgcga cgatttcggg tttatcgagc
    41941 gggctgtcag gaatcggctg gcaggagaca atcaggccac cgttagcagc gattttttga
    42001 tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg
    42061 ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg
    42121 caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc
    42181 aacgctgaac gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcaggc
    42241 tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga
    42301 tgattggggc cagtgcgcca cccaatgcgc caacgttgta ggtaaagccc aggcccgctg
    42361 cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt
    42421 gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa
    42481 tcgcaaatac cggaataatc agcagctgcg aggccagcag gctacaaacg tacgctttgc
    42541 gggttcccag ccagtcaccg aggaagcctc ctacgcagca tcccacggcc gcgccaaagc
    42601 cactaaagaa cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat
    42661 acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca
    42721 tcagcattac gcccgttggc cagcgtttgc ctgtactctg caccataaag ctgataaaga
    42781 ttgcggcgca taacagccca agaacagcga cgatcgcggc attttgcagg ttaccggcga
    42841 agcagaacca cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt
    42901 caccacggta gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt
    42961 tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa
    43021 tgccgataaa gaacagcgca cgccaacccc agaccggaac caccaggcta tagacctgag
    43081 cggcaacgac ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca
    43141 gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc
    43201 ccatgccgat gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg
    43261 ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc
    43321 ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga
    43381 tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca
    43441 gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg
    43501 cgcgccattg tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca
    43561 ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca
    43621 gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc
    43681 tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt
    43741 ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc
    43801 aggccgcgga atacgccagt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc
    43861 agtttctgcg cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc
    43921 cagcccatga tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa
    43981 gcgaagattt cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt
    44041 tccatctgat agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc
    44101 agggtgttga tctgatccag ggtcagtttt accccactca gggctggaat gttgtacaca
    44161 accatcggca aaccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca
    44221 aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta
    44281 gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg ggcgatgagt
    44341 ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg ctcggaaagg
    44401 ctttgtacaa aggcctcgcc ggtcgaacca cccacgtata aaccgtcgat gccctgctga
    44461 atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg ttggtcaaaa
    44521 ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg
    44581 atacttgtct gataaacgat atacctttat acctgttata ccagatcaat taagcaacac
    44641 cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt
    44701 ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc
    44761 acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa tatgctgttg
    44821 atagctaacg ttgttatgct cgtgcagagc ctgatcagcc acggttggac gtgcggcgat
    44881 cagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccag ggatctccgc
    44941 cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg
    45001 actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg catgttcagc
    45061 cgcatagcgc accagactgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc
    45121 accgggatga gaaaggaaat ctttcgccat gccggaaaac tcaccgatga tagtgtccgc
    45181 agaaggacgc gagacgcgag cgcgttcgcc gttgtttatt tgcaccagac ctttgcgttt
    45241 taacgctgcc agcgcttcgc gcacagaagg acgcccgacg ttaaagaacg ccatcagttc
    45301 gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag
    45361 ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac gcaagttgcg
    45421 accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct
    45481 gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga aagtagggga
    45541 aaatggcagg tttttctctt tgtgcctcat cattaccata attaacggaa taattaacta
    45601 ttgcgaaaaa ttaatgtaac gcagataaac acatcccgat tgaattattc ataagactat
    45661 tcacaagcat tatgaatatt atgaatgtgt tcttacaaaa taatcataag cgcatatttt
    45721 ttaatgaaaa atcacatcac ctacaattaa aaacacgaca tccgcaccat aaatagactt
    45781 gcaaaaaata taacatcatt gttttcaatc tgccgtttat gggattgacc gttttctttt
    45841 gacacggagt tcaacaatgt tcggcataat tatatctgtc atcgtattaa ttacgatggg
    45901 ctatttgatc ctgaaaaact acaaacctca ggtggtgctg gctgccgcag gtgtcttcct
    45961 gatgatgtgc ggagtctggt tagggttcgg tggtgtactc gatcccgcca aaagcagcgg
    46021 ctacttgatc gtcgacattt ataatgaaat cctgcgcatg ctgtccaacc gcattgccgg
    46081 attggggctg tcgattatgg cggtgggcgg ttatgcccgc tacatggagc gcataggagc
    46141 cagtcgcgcg atggtgagct tgctaagccg cccgttaaaa ctcattcgct cgccgtatat
    46201 tattctgtcg gcaacttacg tcatcggcca aatcatggcg cagtttatta ccagcgcctc
    46261 cggtctgggt atgttgctga tggtcacctt atttccgacg ctggtgagtc tgggagtaag
    46321 tcgcctctct gcggtggcgg ttatcgcaac cacgatgtcc attgagtggg ggattctgga
    46381 aacgaactcc atttttgcag cccaggtcgc gggaatgaaa attgccacat acttcttcca
    46441 ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc actttttcgt
    46501 gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac gaacaggcag agcaaaaaac
    46561 tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt taatcctgat
    46621 gctcggctcg ctgttcctcg cccacgtcgg gctgatgaag tcagaactgc atctggtggt
    46681 ggtgatgtta ctaagtttga cggtgacgat gtttgttgag ttcttccgca agcataactt
    46741 gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc atgggtacgc agtttgccaa
    46801 cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa ggcttaacga cgattggcac
    46861 tgttgatgcg gttatcaggg gtgcggagca ttctggtctg ggcggtattg gcgtgatgat
    46921 tattatggcg ctagtcattg ccatttgtgc cattgtgatg ggctctggca atgcgccgtt
    46981 tatgtcattt gccagtctta ttccgaatat cgcagccgga ctacatgtac cagcggttgt
    47041 aatgattatg ccgatgcatt ttgccacgac gctagcgcgc gcggtttcgc cgattactgc
    47101 ggtggtgaag cggacagcga tccccatggc agtcggtttc gtggtgaata tgattgccac
    47161 aatcacgcta ttttattaag tcattaaaaa gacaaaacag gccgcctggg cctgttttgt
    47221 attacttcac aacacgtaat gccggtcgac caccgcgtgg tggctgcgga ggttcatcgt
    47281 caggatgagt gtcatcatcg tgatctggct tgtcgccatc aataaccgac ataacggttt
    47341 cgttgtctgc cgatgcctct tcatcattca tgatgctggt atcttcatcg taggcagctt
    47401 caggctcaaa catcgtgcct gcgccatttt cacgggcgta gatagccagc acggcagcca
    47461 gcggcacaga aacctgacgc ggaatgccac caaagcgcgc gttaaagcgc acctcatcat
    47521 tcgccagttc cagattgccg acagcacgcg gcgcaatgtt gagtacgatt tgcccgtcac
    47581 gcgcatattc cataggaacc tgcacgccag ggagcgtcac atccaccacc aggtgcggcg
    47641 tgagctggtt atccagcaac cactcataga atgcacgcag cagataggga cgacgtggtg
    47701 ttagctgtga caaatccata cagattaact ccggcccaga cgcatttcac gttctgcttc
    47761 agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg gtcatatagc ctttcagctc
    47821 tttcgcaccc gggccgctga gctcgatacc cagttgcggc agacgccaca gcagcggagc
    47881 aagatagcaa tcgaccaggc tgaactcatc gctcaggaag tacggcttct gaccgaagac
    47941 cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt gcggcatctg cttcagaagc
    48001 tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct ttttcgatgc gatgcatgta
    48061 cagacggctt tcaccgcgag ctaccgggta aacaggcatc agtggcggat gcgggaaacg
    48121 ctcatccaga tattccataa tgatgcgaga ttcccacagg gtcagctcac gatccaccag
    48181 ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc tgaggcggat tgtccttttc
    48241 cacgtgttcg atctcgaaac ttacaccttt ctcagccagc acaatgcgga cctgatggct
    48301 atagatgtca gtaggaccgg aaaacagcgt cattaccgaa cgtttgttgg cagcgacagc
    48361 catgaaaacc tccaggtata gtcagaattt ttactgctac cagccaccag gtggccagtc
    48421 agaagttgtg ttacccaata aggaacgact ctctttgttc gaaaatcaaa caaaaaatga
    48481 gcaatacccg acatttgggc agaaaattgg atgatagttt accagatttt gcgaccattg
    48541 tggtgagtgg atgccggaaa tggggaaaaa gagatgcgct ttagtctgaa atagttgact
    48601 tagaccctta ttggcgatgt ggtttttgtt ttgcctgtct ttcagacggc agcaaaaagc
    48661 aacttcccat tttttacgct gattcagaat ttagccataa aaaaacccgc cgaagcgggt
    48721 tttttcgtaa attgttttct gccggagcag aagccaatta acgtttggag aactgcggac
    48781 gacgacgtgc tttacgcaga ccgactttct tacgttcaac ctgacgagcg tcacgagtaa
    48841 cgaagccagc tttacgcagt tcagaacgca gggactcgtc gtattccatc agagcgcggg
    48901 tgataccgtg acggatcgca ccagcctgac cagagatacc accaccttta acggtgatgt
    48961 acaggtccag tttctcaacc atgtcgacca gttccagcgg ctgacgaact accatgcggg
    49021 cagtttcacg accgaagtac tgttccagag aacgttggtt gattacgatt ttaccgttgc
    49081 ccggtttgat gaaaacgcga gctgcggaat ttttgcggcg accagtgccg tagtattgat
    49141 tttcagccat tgcctataat cccgattaga tgtcaagaac ttgcggttgc tgtgccgcgt
    49201 ggttgtgctc gttacccgcg taaactttca gtttacggaa catagcacga cccagcgggc
    49261 cttttggcaa catgccttta accgcgattt caatcacacg ctcaggacgg cgagcaatca
    49321 tctcttcaaa gctcacttgt ttgataccac cgatgtggcc ggtgtggtga tagtacactt
    49381 tgtcagtacg cttgttgccg gttacagcaa ctttgtcagc gttcagaacg atgatgtaat
    49441 caccggtatc tacgtgcgga gtgtattccg ctttgtgctt accgcgtagg cgacgagcca
    49501 gttcagtagc cagacggccc agagttttac cggtcgcgtc aacaacatac cagtcgcgtt
    49561 ttacggtttc tggtttagct gtaaaagttt tcattaaaag cttacccaat aaatagttac
    49621 acgttggtga acacccaaac gtcttcaatt gttgaggttc acacgacaaa gtccggcaaa
    49681 cctacccctt cgaatagcct atgccagcac acaaaaagtt ttgggaaaaa aactttcttg
    49741 taacgtgggg tcgcaggatt atagagaagt cggggtcaaa gatcgacccc tttttgtgat
    49801 ttgtgacagg ttttaacccg ccaaatgctc gcgcttcaga tactcttcgc tttgcatctc
    49861 ttgcagacgt gacaggcaac gctggaactc aaacttcagc cgatcgccct gataaatttc
    49921 atacagcggc acttctgcac tcaccactaa tttgacatgg cgctcgtaaa actcatccac
    49981 cagcgcaata aagcgccgcg cttcgctctc catcaaccgc gtcataactg gtacatcaaa
    50041 caacatgacc gtatgaaaga gacgtgagag cgcaatatag tcatgctgac tgcgggcgtc
    50101 gacgcacagc gtagtaaaag agaccgccag cgtctggttc tcgacgccca ttgtcgctaa
    50161 tggccgatgg ttgatttcta acgtcggtga attttctcgt ttcccccccg ccagcgccaa
    50221 ccatagttta tccatttgcg cccgggtttc atcgtggagt ggcgaaagcc acagatgcgc
    50281 ctgagtgagt gtacgcagtc gataatcaac accagcgtcc acgttcatta catcacaatg
    50341 ctgtttaatg gcatcgattg caggcagaaa acgcgcacgt tgcaagccat ttcgataaag
    50401 ttcgtccggc ggaatatttg acgtcgctac cagggtaata ccgcgggcga acaaggcttt
    50461 catcagaccg ccaagtagca tggcatcggt aatatcagaa acaaaaaatt cgtcaaaaca
    50521 gagcacgtca gtttcggctt taaagcgatc ggcaataatt tccagcggat cggtctgccc
    50581 ctgtaaggca gttagctctt catgcacccg cagcataaaa cggtgaaagt gcaggcgctg
    50641 tttccgctct cccggcaggc tttgatagaa aaggtccatc agccaggttt tcccgcgtcc
    50701 tacaccgccc cacatatata agccacgcac tggcgtatgc tttgtgtctt cgcgtttacc
    50761 ccacagctta ccgacccgcg ccattagccc actcgtcctg ggggctggtg gcgtgctatt
    50821 gatgagttcc tgataaataa tttccaggcg gctgacggcc tctttttgaa cgtcgtcggg
    50881 ttgatggctg ccttcattga gcgccttcag gtattgcgat gttggggtaa cgctttgcat
    50941 gatcttattg ttattccttg aataatcggt gcgccgttgt tcacggttga cgaaaaaaag
    51001 gccgttctac actacgcgat atgcagtcgg gattccactt ctgtggaatt aacggttata
    51061 gtggcataat cagccgcagg catggagcct gaagccaaca ccctacggaa acaaaagaca
    51121 acgggagatg ttcatgacct gggaatatgc gctaattggg ttagtcgtcg gcatcattat
    51181 tggtgctgtg gccatgcgtt ttggtaatcg taaactacgc cagcaacagg cgttgcagta
    51241 cgaactggaa aagaataaag ctgaactgga cgagtatcgc gaagagctgg ttagccactt
    51301 tgcccgcagc gcggaattac tggataccat ggcgcacgac tatcgccagc tgtatcagca
    51361 catggcgaaa agctccagca gcctgctgcc ggaactgtct gctgaagcaa acccgttccg
    51421 taatcgtctg gcagagtctg aagccagcaa cgatcaggca ccggtgcaga tgcctcgcga
    51481 ctattctgaa ggcgcatccg gcctgctgcg tactggcgcg aagcgcgact aatttatttt
    51541 tcgggcgcag ccattgcgcc ctcctcttct ctccctcccc gactatcatt taatctggtg
    51601 tctcattgtt agccgtctga aaattcaata acatcaaact gttttgaatc tcttttctta
    51661 tcattcaggt acgagagcag gaataatgaa aaaacaaccc cagctgttga gtgcattagc
    51721 gttaagtgtc gggttaactc tctcggcgtc atttcaggcc gtcgcgtcga ttccaggcca
    51781 ggttgccgat caggcccctc tccccagtct ggctccaatg ctggaaaaag tgcttccggc
    51841 agtggtgagc gtacgggtgg aaggaacggc cagtcaggga cagaaaatcc cggaagaatt
    51901 caaaaagttt tttggtgatg atttaccgga gcaacctgca caacccttcg aaggtttagg
    51961 ctccggtgtc atcatcaacg ccagtaaagg ctatgtgctg accaacaacc atgtgattaa
    52021 tcaggcacag aaaatcagta ttcagctcaa tgatgggcgc gagtttgatg caaaactgat
    52081 tggtagcgat gaccagagcg atatcgccct gttacaaatt caaaacccga gcaaattaac
    52141 gcaaatcgct attgccgact ccgataaatt gcgcgtcggt gattttgccg tagcggtcgg
    52201 taacccattt ggccttgggc aaaccgccac ctctggcatt gtttccgcat taggacgcag
    52261 cgggttgaat cttgaaggtc tggaaaactt tatccagaca gatgcttcca ttaaccgcgg
    52321 taactccggc ggtgcactgt taaaccttaa cggtgagtta attggcatca acactgcaat
    52381 ccttgcgcct ggcggcggga gcgtcgggat tggatttgcc atccccagta atatggcgcg
    52441 aacactggcg cagcagctta tcgactttgg tgaaatcaaa cgcggtttgt taggcatcaa
    52501 aggcaccgag atgagtgccg atatcgccaa agccttcaac cttgacgtgc agcgtggcgc
    52561 gtttgtcagc gaagtgttgc caggttctgg ctcggcgaaa gcgggcgtca aagcgggcga
    52621 tattattacc agcctcaacg gcaaaccgct gaatagcttt gctgagttgc gctctcgtat
    52681 cgcgaccacc gagccgggca caaaagtgaa acttggcctg ctgcgtaacg gcaaaccact
    52741 ggaagtagaa gtgacgctcg ataccagcac ctcttcgtcg gccagcgctg aaatgatcgc
    52801 gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta aaagatggcg gcaaaggtat
    52861 taagatcgat gaagttgtca aaggaagccc agctgctcag gctggcttgc aaaaagacga
    52921 tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt gctgaaatgc gtaaagtgct
    52981 ggcggcaaaa tcggccatca tcgccctgca aattgtacgc ggcaatgaaa gcatctatct
    53041 gctgatgcgt taatgtcgta aaccgggcat caggcttacg tgtgatgtcc ggttaactcg
    53101 tggtatgctg ctgccgttcc cttttttaat gacgcctcca tcatgtttgt gaagctctta
    53161 cgttccgttg cgattggatt aattgtcggc gctattctgc tggttgccat gccttcgctg
    53221 cgcagcctta acccgctttc cactccgcaa tttgacagta ccgatgagac gcctgccagc
    53281 tataatctgg cggttcgccg cgccgcgcca gcggtggtta acgtttacaa ccgtggtttg
    53341 aacaccaact ctcacaacca gcttgagatc cgcaccctgg gatccggtgt aatcatggat
    53401 caacgcggtt atatcatcac caataaacac gtcatcaacg acgccgatca gatcatcgtc
    53461 gccttacagg atggtcgtgt atttgaagca ttgctggtgg gatctgactc tctaaccgat
    53521 ctggcggtac ttaaaattaa tgccactggc ggtttaccta ccattccaat caatgcacgt
    53581 cgcgtaccgc atattggcga cgtggtactg gcgatcggta acccgtacaa cctcgggcag
    53641 accattaccc aggggattat tagtgccacg ggtcgaattg gtctgaaccc gaccgggcgg
    53701 caaaacttcc tgcaaaccga tgcttccatt aaccacggta actctggcgg cgcgctggtg
    53761 aactcgctgg gcgaactgat gggcattaac acgctgtcgt ttgataagag taacgatggc
    53821 gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt tagcaaccaa aattatggat
    53881 aagctgatcc gcgatggtcg cgtgatccgc ggctacattg gcattggcgg acgtgagatc
    53941 gcaccgctgc acgcacaggg cggtggtata gatcaactgc aagggatcgt ggttaatgaa
    54001 gtgtcacctg acggcccggc ggcgaatgcg ggtattcagg tcaacgatct gattatttcg
    54061 gtggataaca aaccggccat ctctgctctg gagacgatgg atcaggtggc ggaaattcgc
    54121 cctggttcgg tgatcccggt tgtagtgatg cgtgatgata agcagttaac gctgcaggtc
    54181 accattcagg aatatccggc aaccaattaa gtcgagtgct caaaacaaaa aaccggagtc
    54241 tgtg
//
LOCUS       DAZPJP010000007        49271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPJP010000007 DAZPJP010000000
VERSION     DAZPJP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.007"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            251..1345
                     /gene="gcvT"
                     /locus_tag="ACHU9J_000374"
     CDS             251..1345
                     /gene="gcvT"
                     /locus_tag="ACHU9J_000374"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1904024.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHU9J_000375"
     CDS             1369..1758
                     /gene="gcvH"
                     /locus_tag="ACHU9J_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1904025.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHU9J_000376"
     CDS             1877..4750
                     /gene="gcvP"
                     /locus_tag="ACHU9J_000376"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1904026.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5016..5759
                     /locus_tag="ACHU9J_000377"
     CDS             5016..5759
                     /locus_tag="ACHU9J_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1904027.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5816..7249)
                     /gene="bglA"
                     /locus_tag="ACHU9J_000378"
     CDS             complement(5816..7249)
                     /gene="bglA"
                     /locus_tag="ACHU9J_000378"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1904028.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7294..7605
                     /gene="yqfB"
                     /locus_tag="ACHU9J_000379"
     CDS             7294..7605
                     /gene="yqfB"
                     /locus_tag="ACHU9J_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1904029.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7769..8428
                     /locus_tag="ACHU9J_000380"
     CDS             7769..8428
                     /locus_tag="ACHU9J_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1904030.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHU9J_000381"
     CDS             complement(8624..9604)
                     /gene="ygfZ"
                     /locus_tag="ACHU9J_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1904031.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHU9J_000382"
     CDS             9847..10113
                     /gene="sdhE"
                     /locus_tag="ACHU9J_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1904032.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10094..10501
                     /locus_tag="ACHU9J_000383"
     CDS             10094..10501
                     /locus_tag="ACHU9J_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1904033.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHU9J_000384"
     CDS             complement(10541..11062)
                     /gene="fldB"
                     /locus_tag="ACHU9J_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1904034.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11174..12070
                     /gene="xerD"
                     /locus_tag="ACHU9J_000385"
     CDS             11174..12070
                     /gene="xerD"
                     /locus_tag="ACHU9J_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1904035.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHU9J_000386"
     CDS             12095..12805
                     /gene="dsbC"
                     /locus_tag="ACHU9J_000386"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1904036.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12811..14544
                     /gene="recJ"
                     /locus_tag="ACHU9J_000387"
     CDS             12811..14544
                     /gene="recJ"
                     /locus_tag="ACHU9J_000387"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1904037.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14635..15733
                     /gene="prfB"
                     /locus_tag="ACHU9J_000388"
     CDS             join(14635..14709,14711..15733)
                     /gene="prfB"
                     /locus_tag="ACHU9J_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1904038.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15743..17260
                     /gene="lysS"
                     /locus_tag="ACHU9J_000389"
     CDS             15743..17260
                     /gene="lysS"
                     /locus_tag="ACHU9J_000389"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1904039.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHU9J_000390"
     CDS             complement(17303..17851)
                     /gene="idi"
                     /locus_tag="ACHU9J_000390"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1904040.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17906..17977
                     /locus_tag="ACHU9J_000391"
     CDS             17906..17977
                     /locus_tag="ACHU9J_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904041.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHU9J_000392"
     CDS             complement(17974..18099)
                     /gene="yqfG"
                     /locus_tag="ACHU9J_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1904042.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18101..19169)
                     /locus_tag="ACHU9J_000393"
                     /pseudo
     CDS             complement(18101..19169)
                     /locus_tag="ACHU9J_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19605..21524
                     /gene="ygfT"
                     /locus_tag="ACHU9J_000394"
     CDS             19605..21524
                     /gene="ygfT"
                     /locus_tag="ACHU9J_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR1904043.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21524..22012
                     /locus_tag="ACHU9J_000395"
     CDS             21524..22012
                     /locus_tag="ACHU9J_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1904044.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22081..22431)
                     /gene="ghxQ"
                     /locus_tag="ACHU9J_000396"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22081..22431)
                     /gene="ghxQ"
                     /locus_tag="ACHU9J_000396"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22467..22781)
                     /locus_tag="ACHU9J_000397"
     CDS             complement(22467..22781)
                     /locus_tag="ACHU9J_000397"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1904045.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22714..25653)
                     /locus_tag="ACHU9J_000398"
     CDS             complement(22714..25653)
                     /locus_tag="ACHU9J_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR1904046.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25650..26429)
                     /gene="ygfM"
                     /locus_tag="ACHU9J_000399"
     CDS             complement(25650..26429)
                     /gene="ygfM"
                     /locus_tag="ACHU9J_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR1904047.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26480..27808)
                     /gene="ssnA"
                     /locus_tag="ACHU9J_000400"
     CDS             complement(26480..27808)
                     /gene="ssnA"
                     /locus_tag="ACHU9J_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR1904048.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27811..30909)
                     /gene="ygfK"
                     /locus_tag="ACHU9J_000401"
     CDS             complement(27811..30909)
                     /gene="ygfK"
                     /locus_tag="ACHU9J_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR1904049.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31231..31809)
                     /gene="mocA"
                     /locus_tag="ACHU9J_000402"
     CDS             complement(31231..31809)
                     /gene="mocA"
                     /locus_tag="ACHU9J_000402"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1904050.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31912..32682
                     /gene="yqeC"
                     /locus_tag="ACHU9J_000403"
     CDS             31912..32682
                     /gene="yqeC"
                     /locus_tag="ACHU9J_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1904051.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32730..34355
                     /gene="yqeB"
                     /locus_tag="ACHU9J_000404"
     CDS             32730..34355
                     /gene="yqeB"
                     /locus_tag="ACHU9J_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1904052.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34396..35328)
                     /gene="arcC"
                     /locus_tag="ACHU9J_000405"
     CDS             complement(34396..35328)
                     /gene="arcC"
                     /locus_tag="ACHU9J_000405"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1904053.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35376..36761)
                     /gene="hyuA"
                     /locus_tag="ACHU9J_000406"
     CDS             complement(35376..36761)
                     /gene="hyuA"
                     /locus_tag="ACHU9J_000406"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR1904054.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36814..38025)
                     /locus_tag="ACHU9J_000407"
     CDS             complement(36814..38025)
                     /locus_tag="ACHU9J_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1904055.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38083..39279)
                     /gene="dpaL"
                     /locus_tag="ACHU9J_000408"
     CDS             complement(38083..39279)
                     /gene="dpaL"
                     /locus_tag="ACHU9J_000408"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1904056.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39337..40524)
                     /gene="ygeW"
                     /locus_tag="ACHU9J_000409"
     CDS             complement(39337..40524)
                     /gene="ygeW"
                     /locus_tag="ACHU9J_000409"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1904057.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41003..42781
                     /locus_tag="ACHU9J_000410"
     CDS             41003..42781
                     /locus_tag="ACHU9J_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR1904058.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42821..43300)
                     /gene="xdhC"
                     /locus_tag="ACHU9J_000411"
     CDS             complement(42821..43300)
                     /gene="xdhC"
                     /locus_tag="ACHU9J_000411"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1904059.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43297..44175)
                     /gene="xdhB"
                     /locus_tag="ACHU9J_000412"
     CDS             complement(43297..44175)
                     /gene="xdhB"
                     /locus_tag="ACHU9J_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1904060.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44186..46483)
                     /gene="xdhA"
                     /locus_tag="ACHU9J_000413"
     CDS             complement(44186..46483)
                     /gene="xdhA"
                     /locus_tag="ACHU9J_000413"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1904061.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46898..47653
                     /gene="actS"
                     /locus_tag="ACHU9J_000414"
     CDS             46898..47653
                     /gene="actS"
                     /locus_tag="ACHU9J_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1904062.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47732..47801
                     /locus_tag="ACHU9J_000415"
     tRNA            47732..47801
                     /locus_tag="ACHU9J_000415"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47764..47766,aa:Gly,seq:ccc)
     gene            48006..>49271
                     /locus_tag="ACHU9J_000416"
     CDS             48006..>49271
                     /locus_tag="ACHU9J_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR1904063.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
BASE COUNT        11956 a        12166 c        13002 g        12147 t
ORIGIN      
        1 ctaatggcat catctttata tagaaactgg tgtgtttttt ggttttttat tctgtcgcga
       61 ttttggcgtt ttttaaccat aagctaatgt gatgatcaat tttaccttat ggttaacagt
      121 ctgtttcggt ggtaagttca ggcaaaagag aacgattgcg ttggggaccg ggagtggctc
      181 cgatgctggg tttcgtggtg ataatttcac catgaaaagt tgtcagcccc gcttattcaa
      241 tgaggacaag atggcacaac agactccttt gtacgaacaa cacacgcttt gcggcgctcg
      301 catggtggat ttccacggct ggatgatgcc gctgcattac ggttcgcaaa tcgacgaaca
      361 tcatgcggta cgtaccgatg ccggaatgtt tgatgtgtca catatgacca tcgtcgatct
      421 ccgcggcagc cgcacccggg agtttctgcg ttatctgctg gcgaacgatg tggcgaagct
      481 caccaaaagc ggcaaagccc tttactcggg gatgttgaat gcctctggcg gtgtgataga
      541 tgacctcatc gtctactact ttactgaaga tttcttccgc ctcgttgtta actccgccac
      601 ccgcgaaaaa gacctctcct ggattaccca acacgctgaa cctttcggca tcgaaattac
      661 cgttcgtgat gacctttcca tgattgccgt gcaagggccg aatgcgcagg caaaagctgc
      721 cacactgttt aatgacgccc agcgtcaggc ggtggaaggg atgaaaccgt tctttggcgt
      781 gcaggcgggc gatctgttta ttgccaccac tggttatacc ggtgaagcgg gctatgaaat
      841 tgcgctgccc aatgaaaaag cggccgattt ctggcgtgcg ctggtggaag cgggtgttaa
      901 gccatgtggc ttgggcgcgc gtgacacgct gcgtctggaa gcgggcatga atctttatgg
      961 tcaggagatg gacgaaacta tttctccttt agccgccaac atgggctgga ctatcgcctg
     1021 ggaaccggca gatcgtgact ttatcggtcg tgaagccctg gaagtgcagc gtgagcatgg
     1081 tacagaaaaa ctggttggtc tggtgatgac cgaaaaaggc gtgctgcgta atgaactgcc
     1141 ggtacgcttt accgatgcgc agggcaacca gcatgaaggc attatcacca gcggtacttt
     1201 ctccccgacg ctgggttaca gcattgcgct ggcgcgcgtg ctggaaggta ttggcgaaac
     1261 ggcgattgtg caaattcgca accgtgaaat gtcggttaaa gtgacaaaac ctgtttttgt
     1321 gcgtaacggc aaagccgtcg cgtgatttac ttttttggag attgattgat gagcaacgta
     1381 ccagcagaac tgaaatacag caaagaacac gaatggctgc gtaaagaagc cgacggcact
     1441 tacaccgttg gtattaccga acatgctcag gagctgttag gcgatatggt gtttgttgac
     1501 ctgccggaag tgggcgcaac ggttagcgcg ggcgatgact gcgcggttgc cgaatcagta
     1561 aaagcggcgt cagacattta tgcgccagta agcggtgaaa tcgtggcggt aaacgacgca
     1621 ctgagcgatt ccccggaact ggtgaacagc gaaccgtatg caggtggctg gatctttaaa
     1681 atcaaagcca gcgatgaaag cgaactggaa tcactgctgg atgcgaccgc atacgaagca
     1741 ttgttagaag acgagtaacg gctttattcc tcttctgcgg gagaggatca gggtgaggaa
     1801 aatttatgcc tcaccctcac tctcttcgta aggagagagg ttcacaattc actgcacgtt
     1861 tcaggaacca tcgctcatga cacagacgtt aagccagctt gaaaacagcg gcgcttttat
     1921 tgaacgccat atcggaccgg acgccgcgca acagcaagaa atgctgaatg ccgttggcgc
     1981 acaatcgtta aacgcgctga ccggccagat tgtaccgaaa gatattcagc ttgcgacccc
     2041 gccgcaggtt ggcgcaccgg cgaccgaata cgccgcactg gcagaactca aggctattgc
     2101 cagtcgcaat aaacgcttca cgtcttacat cggcatgggt tacaccgccg tgcagctacc
     2161 gccggttatc ctgcgtaaca tgctggaaaa tccgggctgg tataccgcgt acactccgta
     2221 tcaacctgaa gtctcccagg gccgccttga agcactgctc aacttccagc aggtaacgct
     2281 ggatttgact ggactggata tggcctctgc ttctcttctg gacgaggcca ccgctgccgc
     2341 cgaagcaatg gcgatggcga aacgcgtcag caaactgaaa aatgccaacc gcttcttcgt
     2401 ggcttccgat gtgcatccgc aaacgctgga tgtggtccgt actcgtgccg aaacctttgg
     2461 ttttgaagtg attgtcgatg acgcgcaaaa agtgctcgac catcaggacg tcttcggcgt
     2521 gctgttacag caggtaggca ctaccggtga aattcacgac tacactgcgc ttattagcga
     2581 actgaaatca cgcaaaattg tggtcagcgt tgccgccgat attatggcgc tggtgctgtt
     2641 aactgcgccg ggtaaacagg gcgcggatat tgtttttggt tcggcgcaac gcttcggcgt
     2701 gccgatgggc tacggtggcc cacacgcggc attctttgcg gcgaaagatg aatacaaacg
     2761 ctcaatgccg ggccgtatta tcggtgtatc gaaagatgca gctggcaata ccgcgctgcg
     2821 catggcgatg cagactcgcg agcaacatat tcgtcgtgag aaagcgaact ccaacatttg
     2881 tacttcccag gtactgctgg caaacatcgc cagcctgtat gccgtttatc acggcccggt
     2941 tggcctgaaa cgtatcgcta accgcattca ccgtctgacc gatattctgg cggcgggcct
     3001 gcaacaaaaa ggtctgaaac tgcgccatgc gcactatttc gacaccttgt gtgtggaagt
     3061 ggccgacaaa gcgggcgtac tgacgcgtgc cgaagcggct gaaatcaacc tgcgtagcga
     3121 tatcctgaac gccgtgggga tcacccttga tgaaaccacc acgcgtgaaa acgtaatgca
     3181 gcttttcagc gtgctgctgg gcgataacca cgggctggag atcgacacgc tggacaaaga
     3241 cgtggctcac gacagccgct ctatccagcc tgcgatgctg cgcgacgacg aaatcctcac
     3301 ccatccggtg tttaatcgct accacagcga aaccgaaatg atgcgctata tgcactcgct
     3361 ggagcgtaaa gatctggcgc tgaatcaggc gatgatcccg ctgggttcct gcaccatgaa
     3421 actgaacgcc gccgccgaga tgatcccaat cacctggccg gaatttgccg aactgcaccc
     3481 gttttgcccg ccggaacagg ctgaaggtta tcagcagatg attgcgcagc tggctgactg
     3541 gctggtgaaa ctgaccggtt acgacgccgt ttgtatgcag ccgaactccg gcgcacaggg
     3601 cgaatacgcg ggcctgctgg cgattcgtca ttatcatgaa agccgcaacg aagggcatcg
     3661 cgatatctgc ctgatcccgg cttctgcgca cggaactaac cccgcttctg cacatatggc
     3721 aggaatgcag gtggtggttg tggcgtgtga taaaaacggc aacatcgatc tgactgatct
     3781 gcgcgcgaaa gcggaacagg cgggcgataa cctctcctgt atcatggtga cttatccttc
     3841 tacccacggc gtgtatgaag aaacgatccg tgaagtgtgt gaagtcgtgc atcagttcgg
     3901 cggtcaggtt taccttgatg gcgcgaacat gaacgcccag gttggcatca cctcgccggg
     3961 ctttattggc gcggacgttt cgcacctcaa cctgcataaa actttctgca ttccgcacgg
     4021 cggtggtggt ccgggtatgg gaccgatcgg tgtgaaagcg catctggcac cgtttgtacc
     4081 gggtcatagc gtggtgcaaa tcgaaggcat gttaacccgt cagggcgcag tttctgcggc
     4141 accgttcggt agcgcctcta tcctgccaat cagctggatg tacatccgca tgatgggcgc
     4201 agaagggctg aaaaaagcaa gccaggtggc aatcctcaac gccaactata ttgccagccg
     4261 cctgcaggat gccttcccgg tgctgtatac cggtcgcgac ggtcgcgtgg cgcacgaatg
     4321 tattctcgat attcgcccgc tgaaagaaga aaccggcatc agcgagctgg atattgccaa
     4381 gcgcctgatc gactacggtt tccacgcgcc gacgatgtcg ttcccggtgg cgggtacgct
     4441 gatggttgaa ccgactgaat ctgaaagcaa agtggaactg gatcgcttta tcgacgcgat
     4501 gctggctatc cgcgcagaaa ttgaccaggt gaaagccgct gtctggccgc tggaagataa
     4561 cccgctggtg aacgcgccgc acattcagag cgaactggtc gccgagtggg cgcatccgta
     4621 cagccgtgaa gttgcggtat tcccggcagg cgtggcagac aaatactggc cgacggtgaa
     4681 acgtctggat gatgtttacg gcgaccgtaa cctgttctgc tcctgcgtac cgattagcga
     4741 ataccagtaa ttcactgatt cgactatttt ctaaaggcgc ttcggcgcct ttttagtcag
     4801 atgacaaagt acaaaagtgc tcagacagtc ccctcgcccc tttggggaga gggttagggt
     4861 gaggggaaca ggccggcact ggcgcgaata tttaccctca ccccggccct ctccctgaaa
     4921 gggcgagggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc aatctcaaag
     4981 atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt gtgaccgatg
     5041 gcagtcgcgg catcgggcgg gcaactgcat tactgttggc gaaagaagag tatacggtgg
     5101 cggttaatta tcaacaaaat ctccatgcgg cgcaggaagt ggtgaactta atcacgcaag
     5161 ccggtggcaa ggcattcgtg ctccaggcgg atatcagcga cgaaaatcag gtcatcgcaa
     5221 tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat aacgccggga
     5281 tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt
     5341 ccaccaacat gacgggatat tttctctgct gccgcgaggc ggtaaaacgc atggcgctta
     5401 aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca cggttgggtt
     5461 cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg ttaaccaccg
     5521 gactatcgct ggaagtcgcc gcgcaggggg tccgcgttaa ctgcgtgcgg ccagggttta
     5581 tttttaccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc gttaagtcga
     5641 acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt gtctggctac
     5701 taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc gggaaataaa
     5761 acagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca tcgacttaaa
     5821 gcttctcgcc gttgctggta atcacctctt tgtaccagtt aaagctcttc ttacgtgaac
     5881 gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg tagcgtttgc
     5941 tgtactgccc ggtggtgaac gatacgcagt cgatgcagcc ccacggtgtg tagcccatca
     6001 gatccacgcc atcgtaagtc accgcttttt tcatctcttc gatatgggcg cgcaggtagt
     6061 caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa gcgccaaaac
     6121 cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa agggcatagc
     6181 gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac gggttcggta
     6241 cgctgccttc gaaaccagag atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg
     6301 tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca tcgagatcgc
     6361 cgtcttccat tttgatgtta aatccgcgac gctcccactc gttcaacaca taggacgggt
     6421 aatagccgcg tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga
     6481 acatcacatc gtccgggtta cagcagtagg gatagagcgg caccatcgcc agcatacagc
     6541 cgactttcat ctccgggtta atgcgacgcg cagctttcac cgccagggcg ctggcgacaa
     6601 actggtgatg cagcacctga tacatcgtct cttccgggtt ctcatgctcg gtataaacca
     6661 cgccggagca gcagtaaccg aacagcggtg cacgccagtt acgctggttg ttaatttcgt
     6721 tgaaggtcat ccagtatttg actttatgct tatagcgttc aaacaccact tccgcgaaac
     6781 ggacaaagaa atcaaccact ttacggttgg tccagctacc gtattgctgc accagatgca
     6841 gcggcatttc aaagtgggag agggtgatca ccggttcgat gttgtatttc agcagttcat
     6901 cgaacatatc atcgtagaac ttcagccctt cttcgtttgg ctgagcttca tcgccttttg
     6961 gaaaaatgcg cgtccaggct atggaagtcc gaaaacattt gaagcccatt tcggcaaata
     7021 gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt ggatagtatt
     7081 tgcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca ccggtcagaa
     7141 cgtcacaaat gctcggccct ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg
     7201 caactgcgcc gccccataag aaatctttct gtaaggtgag ttttttcact atcataggct
     7261 ccagatgagt caattcttac aggaaatagt accatgcagc caaacgacat cacttttttt
     7321 caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg cgacgagtct
     7381 gaatcgcact tcaaaacggg tgatgtgctt cgtgtcgggc gttttgaaga tgacggttat
     7441 ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacgct gacggaaaaa
     7501 catgcagagc aggaaaatat gaccctgact gaactgataa aggtcattgc cgacatctat
     7561 cccggtcaga cacagtttta tgtgattgaa tttaaatgtc tttaaattga actcacgcct
     7621 gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc aaatcagaat
     7681 ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag aggtatacat
     7741 gtgcgtgtac gtttctccgg agtaagttat ggttcagaag cccctcatta agcagggata
     7801 ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggttgg tgtttggtat
     7861 cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg ccacagcgat
     7921 aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt cgacgctcta
     7981 tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc attgcgctat
     8041 ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg attctccgtt
     8101 agcgcgcggg ttgatgattg ttatctggag cctggcattg ctgggtattc tgtttaaact
     8161 gaccatcgcg caccgattca aaattttatc tctggtgacc tatctggcga tgggctggct
     8221 gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg ttaccttatt
     8281 ggcggtaggc ggcgtggttt attcgctcgg agtgattttc tatgtctgca aacgcattcc
     8341 ctacaatcat gccatctggc acggcttcgt gctcggcggc agtgtgtgcc actttctggc
     8401 gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg aaaagacgcg
     8461 tcagcgtcgc atcaggcatc ggagcacaac tgccggattc ggcgtgaacg ccttatccga
     8521 tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga taagacgcgt
     8581 cagcgtcgca tcaggcgata aatgcctgat gcgataaaga gatttactct tcgagcgaat
     8641 acggcagcgg ctcaatatgc aatgtattcg catcgtcgcg tacgcggaag atgctatccg
     8701 gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc agttttaccg
     8761 cagccagcac cgtaccggta cgacgccagt tctcgcccat tttcagctct aagtcttcac
     8821 cagcttccgg cagtcggctg gcgctacctg ccagcaacca gagcgcacgt ttattggcac
     8881 cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc ttaaagctga
     8941 taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg ttggcggcat
     9001 caatcaccgg gaaacccgct tcaatgttta atgccagcca ctgttggcta ttgttcagtt
     9061 ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg gttacgatca
     9121 ggaaacgttc tgccgggtat tcaaaccata gcaaagtggt cgcgccttct ttaactacct
     9181 gtttttcttt cgaaggcagt tcactaaaga tatttgccag cgcggcgcgc gcctgaaaac
     9241 cggcaacacc aagcagcaca tgctcgtcgt ctggcgcgat ggtcacttta gagaacaccg
     9301 catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca atccacgcaa
     9361 agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttgcctttg gcgtcgcaat
     9421 gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc acctgaccct
     9481 gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca tcaagcgtca
     9541 tcagcgtcag cggtaaacgg gcagaagccg taggctgacg gggaggaaaa ggtgtaaaag
     9601 ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa aagagctatg
     9661 aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac ttccaaaaaa
     9721 atgaagccat tgaattgttg tgaattaatt tcgtgatcgc gatggtgttt actgatattc
     9781 ccgtaaaaga aggcgaaaaa cacgttacaa tagcggggta ctggattttc gtgagacagg
     9841 aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg tggtatgcgc
     9901 gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt aagcgatgac
     9961 gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt taactggctg
    10021 atgaatcacg gtaaaccggc cgatgcagaa ctggaaatga tggtccgact catccagaca
    10081 cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc gcgcacagtg
    10141 gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc cctggccact
    10201 cagttacacc ccgttatgga tggtgttact ttcgctggtg gtgtttgatt gcgttcgcag
    10261 ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg ggcgtttgcg
    10321 ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga gcggcatgat
    10381 gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag ccgacagcat
    10441 ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga cgcaaagata
    10501 aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa tgctctgcca
    10561 tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg tcatactggt
    10621 tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc ggtttcgggc
    10681 tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct ttggtcgaga
    10741 gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc agttgatcgc
    10801 caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc tgatcccaga
    10861 cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc agaatgagca
    10921 catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag gtcaccagtt
    10981 ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag gtgctggaac
    11041 cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc ttttgcagct
    11101 agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa atgagtgacc
    11161 ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg atgctctgtg
    11221 gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc tgtcaatgat
    11281 ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg acgatttgca
    11341 ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag cgcgtttgct
    11401 gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg aagacgatcc
    11461 cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt taagcgaagc
    11521 gcaggtcgaa cgtttattac aggcgccatt aattgatcag ccactggagc tacgcgataa
    11581 agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg tcgggctgac
    11641 aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag gcaacaaaga
    11701 gcgcctggta ccgttaggtg aagaggcggt ttactggctg gaaacctatc tggaacatgg
    11761 gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc agcgtgcgca
    11821 gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc tggcgggtat
    11881 cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc atttattaaa
    11941 tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc tttccaccac
    12001 gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac agcatcaccc
    12061 gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta tgttgtttac
    12121 tttgttagcg gcgttttcag gttttgctca ggctgatgac gcggcaattc aacaaacgtt
    12181 agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg gcatgaagac
    12241 agttctgact aacagcggcg tgttgtacat caccgatgat ggtaaacata tcattcaggg
    12301 gccaatgtat gacgttagcg gcacggctcc ggtcaatgtc accaataaga tgctgttaaa
    12361 gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgctgcagg aaaaacacgt
    12421 catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg agcaaatggc
    12481 agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc aggggctgga
    12541 cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa acaaagcgtt
    12601 tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg atattgccga
    12661 ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg tgctgagcaa
    12721 tggcacactt gttccgggtt accagccgcc gaaagagatg aaagaattcc tcgacgaaca
    12781 ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact tcgtcgccgt
    12841 gcagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg ccgtttatac
    12901 gccagccggg gcgtgcgcag tgcgcaagaa ctggaacgca gtgttaaagg gatgctgccc
    12961 tggcagcaac tgagcggcgt cgaaaaggcc gttgagatcc tttacaacgc ctttcgcgaa
    13021 ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gtgccaccag cacggctcta
    13081 agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt tccaaaccgt
    13141 ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc ccgtggtgcg
    13201 cagttaattg tcacggtgga taacggtatt tcctcccatg cgggggttga acacgctcgc
    13261 tcgttgggca ttccggttat tgttaccgat caccatttgc cgggcgaaac attacccgcg
    13321 gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa atcactggca
    13381 ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg cgatcagggc
    13441 tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga tctggtagcg
    13501 ctggggacag tggcggacgt cgtaccgctg gacgctaata atcgcattct gacctggcag
    13561 gggatgagtc gcatccgtgc cggaaagtgc cgtccgggga ttaaagcgct gcttgaagtg
    13621 gcaaaccgtg atgcacaaaa actcgccgcc agcgatttag gttttgcgct ggggccacgt
    13681 ctcaatgctg ccggacgact ggacgatatg tccgtcggtg tggcgctctt gctgtgcgac
    13741 aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca gacgcgaaaa
    13801 gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact ggagcgcagc
    13861 cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca gggggttgtc
    13921 ggtattctgg cttcccgtat taaagagcgt tttcaccgtc cggttatcgc ctttgcgccc
    13981 gcgggtgatg gtacgctgaa aggttctggt cgctccattc aggggctgca tatgcgtgat
    14041 gcgctggagc gattagacac actctaccct ggcatgatgc tgaagtttgg cggtcatgcg
    14101 atggcggcgg gtttgtcgct ggaagcggat aaattcgaac tctttcaaca acggtttggc
    14161 gaactggtta ctgagtggct ggacccttcg ctattgcaag gcgaagtggt gtcagacggt
    14221 ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga tgctggcccg
    14281 tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccggctgct gcaacagcgg
    14341 ctggtgggcg aacgtcattt gaaagtcatg gtcgaaccgg tcggcggcgg tccgctgctg
    14401 gatggtattg cttttaatgt cgataccgcc ctctggccgg ataacggcgt gcgcgaagtg
    14461 caactggctt acaagctcga tatcaacgag tttcgcggca accgcagcct gcaaattatc
    14521 atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg tggattgggt
    14581 acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca gaccatgttt
    14641 gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga cgttcttagg
    14701 gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg ccgagctgga
    14761 acagccggat gtctggaatg aacccgaacg cgcacaggcg ctgggtaaag agcgttcctc
    14821 cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag atgtttctgg
    14881 tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag ccgttgctga
    14941 actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt tctctggcga
    15001 atatgacagc gccgactgct acctcgatat tcaggcgggg tctggcggta cggaagcaca
    15061 ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc gtggtttcaa
    15121 aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg tgacgatcaa
    15181 aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc accgcctggt
    15241 gcgtaagagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct ctgcgtttgt
    15301 ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc tgcgcattga
    15361 cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat ctgcggtgcg
    15421 tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt cccagcacaa
    15481 gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg agatgcagaa
    15541 gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct ggggcagcca
    15601 gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgtaccg gggtagaaac
    15661 tcgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag caagtttgaa
    15721 agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg acgcggtagt
    15781 cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc gcgagcaggg
    15841 gattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc acgcagaatt
    15901 cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg ttgctggccg
    15961 catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg atgtgggtgg
    16021 ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca acgagcagtt
    16081 caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca aaaccaaaac
    16141 cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac tgcgtccgct
    16201 gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc gttatctgga
    16261 tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga tcctctctgg
    16321 tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga tgatgcaggt
    16381 gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc tggatctcga
    16441 catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg gtggcttcga
    16501 gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac gtcataaccc
    16561 agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc tgatcgagct
    16621 gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg aagtgaccta
    16681 cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc gtgaagcgat
    16741 caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact ctgcgaaagc
    16801 gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc gtatcgttac
    16861 cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca ttactgaata
    16921 tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa tcacagaccg
    16981 ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc tgaatgacgc
    17041 ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag gtgacgacga
    17101 agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac cgccgacagc
    17161 aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc ataccatccg
    17221 cgacgttatt ctgttcccgg cgatgcgtcc ggtaaaataa gcattacgtt atgctcacaa
    17281 ccccggcaaa tgtcggggtt ttttaattgt gctgcgcgaa agcagacaac aattttcttg
    17341 cttcactatt ggctgcctgc atcaccatcc acggactgaa cgcccacggt gtggcatcaa
    17401 taccgtgtaa tacatctgct aaatcacacc attgataatc catcacttca tcatcgttga
    17461 tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt tccacaatgc
    17521 cattcggatc ggtggcgcga tagcgaaagt caggatagat agattcagga ggcgtaattt
    17581 ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgttgctt tctcccagtt
    17641 gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg ctcagtgcgc
    17701 ggcgggtaac taataattgt cctttggcat taaacagcca actggagaac gcgagatgta
    17761 agcgggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga actccctgtg
    17821 cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc tgatcactca
    17881 tcattataga taattgaaga gtgaaatgat taaccaagtg agcgtttatc gacaaccgcc
    17941 cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca tatatttgaa
    18001 gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga gagtaaaaag
    18061 caacagcaga ctgaatataa cgcgcattaa aaaattcata ttattctcca tgctcatttt
    18121 tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa tctttatcag
    18181 agacggattc cttcaccagg tcagcatgat gctggtagcc attaaagaag acattcagca
    18241 caacggcgct aagggttgcg agcataatgc cgctatgcag caacggttgc agtacggccg
    18301 gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg agactgatcg
    18361 ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga attcgaatcc
    18421 ctgtcgccag taccatgccg aacatcacca gcccagcaca aattgcggaa tggaggcgac
    18481 cagcaccgcc atttttggca ccatgccaaa caggattaat ataattcccg aagaaataca
    18541 cacccagcgg ctataaatcc tttcgcatag cgagtaatga gcaagataaa aattaagaca
    18601 gcaaaggaga tacctaaata aacgggatta ccatattgcg gattcccttt acctccggcg
    18661 gcccagtcaa tacccacctg aatgatgcta agcccaatag aagtaataac cacaccggta
    18721 accagtggcg ggaataaagg catcaagcga ccaataaatg gcgctaataa tgtggtgata
    18781 aaacccgcgg cgatagtggc accaaatatc cccagcaggc cgatatccgg gttcatccca
    18841 atggctatca ttggtgttac agcagcaaag gtcaccgaca taatcaccgg caggcggatc
    18901 cccataaagc ggccgatacc gatacattgc aataatgtga cgatcccgca gcaaaagaga
    18961 tccgagctaa tgagcatcgc aatagcatct tttgagaggc ccagtcggtc accaatcata
    19021 agaggaacag cgactgcacc tgcgtacatg acaaggacgt gttgcagacc gagtatgatc
    19081 agctttcctg gtgataatat gcggtcaacc tcatcagttg ggcggtcttg cccagaagat
    19141 gagggaagtt gggaatctat ggcgctcatc agtgtttctc ctttcgcccg ccagagtaaa
    19201 aagcaaccgt ttacgttaat gaaactgtct aaacacatgc tcgtggagtg ccagtttttg
    19261 cgaggaaggg gagttagaga caggaattgc ggaggcaatc acataatcgt gcttaaacca
    19321 cgaaaatgac aagagaacct gacaatggaa ttatctgtaa ttttctcata ttgatatttc
    19381 attaatcatt ctggcatgtc gcactctagc atttaatcgt ttttatctgg atagcgctct
    19441 tttgatcggc aatgttgaaa taatgaaagc ggcatagatt gaggggcaat aatttgaact
    19501 cagtcgcaaa attataatta aaacggtcat gctacctaat atatgcccgt ttattgcatc
    19561 gcgaaatttg agaagacata actcattaaa tgaaagggat gcaaatgaat aagtttatcg
    19621 ctgctgaagc tgcggaatgt ataggctgtc atgcttgtga aattgcctgt gcggtggcac
    19681 acaatcaaga aaactggccg ctgagtcaca gtgactttcg accgcgtatc cacgttgtag
    19741 ggaaaggcca ggctgcgaat ccggtggcct gccatcactg caacaatgcc ccttgtgtta
    19801 cggcttgtcc ggttaatgct ctgactttcc agtccgatag cgtacaactg gacgagcaaa
    19861 aatgtattgg ctgtaaaaga tgcgcaatcg cttgcccctt tggcgtcgtt gagatggtcg
    19921 atacgattgc gcagaaatgc gacctttgta accagcgcag ttccggcacg caagcctgta
    19981 ttgaagtctg cccaacacag gcgttacgac tgatggacga taaagggtta cagtggataa
    20041 aggtggctcg ccagcgcaaa acggcagcag gaaaagcgtc atcagacgct cagccatctc
    20101 gcagtgcagc gttgctcccc gttaactcgc gtaaaggcgc agataaaatt tcagcgagtg
    20161 aacggaaaac ccactttggc gaaatctatt gcgggctgga tccactgcaa gcgacttatg
    20221 agagtgaccg ctgtgtttat tgtgccgaaa aagctaactg caactggcat tgcccgctgc
    20281 ataacgctat tccggattac atccgtctgg tacaggaagg aaagattatt gaagcggcag
    20341 aactttgcca ccagaccagt tccttaccag aaatctgcgg cagggtatgc ccacaggacc
    20401 gtctttgtga aggcgcatgt actttgaaag atcactctgg cgcagtctct atcggtaatc
    20461 tggaacgcta catcaccgat accgcgctgg cgatgggctg gcgtcccgat gtcagcaaag
    20521 ttgttccccg tatcgaaaaa gtggcggtga ttggcgctgg gccagcaggg ttagggtgtg
    20581 ctgatattct ggcgcgcgcg ggagttcagg ttgatgtctt tgatcgccat ccagaaattg
    20641 gcggtatgct gacttttggc attcctcctt tcaaactcga taaaacggta ttaagccagc
    20701 ggcgagagat attcaccgca atgggaatcg actttcatct taactgtgaa attggccgcg
    20761 atattacctt tagcgattta acttctgaat atgatgcagt tttcatcggc gtggggactt
    20821 acgggatgat gcgagcagat ctgccgcatg aagatgcgcc cggtgtcatt caggctctac
    20881 cgttcctgac tgcccatacc cgccagctca tgggattgcc ggagtctgaa gagtatccgc
    20941 tgacggacgt ggaaggtaag cgagtcgtgg tattgggcgg tggcgatacg acaatggatt
    21001 gtttgcggac ttccattcgc ctcaatgctg ccagcgtgac ctgcgcgtat cgtcgtgatg
    21061 aagtcagtat gccgggctcg cgcaaagagg tggttaatgc gcgcgaggaa ggtgtcgagt
    21121 ttcagttcaa tgttcaaccg caatatatcg cttgtgacga agatgggcgt ttaactgcgg
    21181 tgggcctgat tcgtaccgcc atgggtgagc cggggccgga tggtcgccgt cgtcctcgtc
    21241 cggtagcggg ttcagagttt gaattgcccg ccgatgttct gattatggcc tttggtttcc
    21301 aggcgcatgc catgccgtgg ttgcagggca gcggaattaa actcgataaa tggggcctga
    21361 ttcaaaccgg tgacgtcggg tatttaccta cccagacgca tctgaaaaaa gtctttgctg
    21421 gtggtgatgc agttcatggc gcggatctgg ttgtcactgc aatggccgca ggaaggcagg
    21481 cggcgcgcga tatgttaact ctgtttgata cgaaggcatc gtgatgaaat cgttaattat
    21541 cgttaatccg gctgactgta ttggctgccg tacttgtgaa gtagcctgtg tggtcgctca
    21601 cccttcagaa caggagttga atgccgatgt ctttttaccc cggctgaagg tacagcgact
    21661 ggacagcatc agtgcgccgg tgatgtgcca tcagtgtgaa aacgcccctt gtgttggcgc
    21721 ttgccccgtg ggggcgctga cgatgggcga gcaggtggtg cagaccaatt ctgcccgttg
    21781 tattggctgt cagagttgcg tcagtgcgtg cccgtttgga atgatcacca ttcagtcgtt
    21841 gccgggggat acccgacaac aaattgtgaa atgcgatctc tgtgaacagc gggaagaggg
    21901 gccagcctgt gttgaatctt gcccgacaca ggcgctgcag ttgcttactg aaagagaact
    21961 caggcgagtc cgccagcagc gtattgttgc cagcggtgag aatcctctgt gaaagaaatc
    22021 tccgcgctga aaggcgcgga gaatctatta gattgcccaa ccacccgcgt aaaatgcgac
    22081 cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga ctgaatgcgg taaatgccgt
    22141 caaggaaatc gcacaaccaa tcgccattgg caaattggcc tacaacccca tcagcaacga
    22201 gccgaagccc gcgaccagac aggtggcaac aaacacagct gcgggaggaa aacctgcttt
    22261 gcccagcatt cccggaacga cgataacgga ataaaccatg gccagaaagg tcgttaagcc
    22321 agccagtact tcctgacgaa cggtactgcc acgagcggta attttaaaat aattatccag
    22381 cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg tctccagaca taatgatgtc
    22441 ctcacgagga tgttaaagtt ttattattag ttgcgttcgt acaccagacg accatcaacg
    22501 taggtgcggt agatcgaacg gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact
    22561 aaagaaacag agttgtcata gcgcagctgc tgtagcggag tggcggtggg ttccatcacc
    22621 acgaaatcag cctctttgcc aggtaaaaag ttgccaatca aatcgtcaag gcccagagat
    22681 ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg ccgagaggcg atagccttgt
    22741 aattgcaaca ctttgtaggc ttcgttcagc gtttgcagca tgttgaaagt tgttccggca
    22801 ccgatatccg ttcccatgcc cactttgact ttcttctgcc cagatgccgc atgcatcgtg
    22861 aattgcggta gcaatcgccg gagctgcgcc atttacaccg atttccgaga tcgatttcgc
    22921 cccgaacggg ccgactttat cgtcgctcgg caccagcaca gcgcggaaat cacgcggaat
    22981 gtcaccaatt ttcggtgcgc cgtaactgcg taaatcacgc gttaacgggt gaccttcggc
    23041 gtcataaatg atctcttcgc tcatactgtg gccgatagct cgcagggtag caccgtagat
    23101 ttgtcccaat gctaattccg ggttgaccgg tgtaccgcag tccagcaagg cgtagaattt
    23161 atccaggcgg atttcacccg tacgcgtgtt gacggcaact tcagcgaagt ttgcgccata
    23221 cgggaaggcg aaatcaggcg tgatataact gccagttccc accagtgaac caaagccgat
    23281 gccggtttcg cctttatggg caatatcccc gaaactaact tcgcctttct tgccgcgcac
    23341 gacgcccggc gttgccagtt gaacatctgc cactgcctca cctaacattt gcgcgccgtg
    23401 gaacagaatt ttctcccgta gattttccgc tgccaaacgc gccgcgttac ccgagaagca
    23461 agtaccggac gaggcatatg cgcctttatc aaacaacgca tgatcggtat caccggagat
    23521 aacatgcacg tcctgcggtg ggcagtgcag cacttctgct gccagtttcg tcactacagt
    23581 atccagacca gtaccaatat ccgcaccgcc agaatgaacg ataaaggtgc cgtccgattc
    23641 cagtttgatc atgcagttag cctgatcgat atccgggata cccgatttct gcatgataat
    23701 ggcgacgccg cgaccgatgt gccagtcacc attttgcggt ttcggtgacg accattggat
    23761 catctcgcga ccctgacgca ggatctcttc cagtgcacag ctggcggcgg aaggaacgga
    23821 ggtcggcgct ttaccttcac cgattgcacc gagaattttc agctcttgcc cttcgtgtac
    23881 ccggttacgt tcgataattt ccagttggtc gatctgtaac tgttcagcca gttccgctaa
    23941 tgccatggtg atagcgaagt tacctttcgg tgcgccataa ccctgataag caccatttgg
    24001 gcaaatgttg ctgtagtagg tggtgacctg gaaatcgacg ttatcgcacg gatataacgg
    24061 cagcgacagc gccggtccgt tacacggtac ggtgagtgag tggttgccgt aagggccagt
    24121 gttggcgcgg aaatccatct tcactgccgt caggcgacca tcttttttcg ctcccagttt
    24181 gacggtgact ttcgcgacgt gacgagaggt gttagcaata aactcttctt cacgggtgta
    24241 gcggaatagt accggacgcc cggtcacgca ggttgcccag gcgcacactt cttccagcag
    24301 gatgtcctgt ttggaaccaa aaccgccgcc aactcgctct ttaatgacat gaactttatg
    24361 ctgtttcatg cccacgaggc gcgcgacctg gcggcgtaag tgccatggta cctgggtgga
    24421 ggcgtggata accagacgat cgccgtccat ccgggtaaag cagatatgtg tttcagtcgg
    24481 gcactgctgt gcctgcgttg agttataggt tcgctcaatg atcacatcgg catcggcaaa
    24541 gcctttgtcc atatcgccaa tatgaccatg aatactggcg gcgatatttt tgcgagggcg
    24601 agaaccgatc gggaagttga tgatcatatg ctcgccgcgc tgggctgcat ggctgttatc
    24661 gtcttccaga gtatctggcg caccagcaac atacaccacc ggttcatcgt gcacgacagg
    24721 cgcatcttcc gccattgctt cgtcgatcga cattaccggc ttaagcactt catattcaac
    24781 gtcgatgagc ttcagtgctt cgagcgcaat ttcttcactt tctgcgacga ccgcagcaac
    24841 acgatcgccg acgtgacgca ttttcttgcc gaacatacgg cggtcaagcg gtgacggttc
    24901 cggtgcgctc tgaccccccg gggtatagta gatatcaggg caattcaggt gagtaataac
    24961 gtgaacgacg cccggtaagg cttcagcttt gctgacatcc agatgagtaa tcagtgcgtg
    25021 agcgtgtggg ctacgtaaca ttttaatgac gcaggcgtca gccgttacgc agtcttcaac
    25081 atagcagggt ttcgcctgca ccattttcgc ggcatcagtt ttaggataat gcttgccaat
    25141 gacgtcaagg tcgtcacgga aagtcggagc gatatcaatg gtggcctgcg gattattttt
    25201 acgtgcaacc gccagttcaa tgacctgata gtattgttgc cagcctgcat cgcggctgaa
    25261 caaaccagaa agcgcgtcgt cgatctcttc gcgggtaggt gcggcgatgc gatcgagaag
    25321 atcggtgata atcagagctg cagctggatc gttataacca gactgcacca cgccaacatc
    25381 aaccattgcc tgttgaacca gacttaactc gttccattta cccagagatt ctgcggtacg
    25441 aatatctgcc ttctctaact gtgcggcaat aagcaaggac gcgttaacga tattaccgtt
    25501 aaagattatt gcgtcagaac cggcaaaccc gaagccatca tcactgttgc gtacagagtg
    25561 cattcccatg ttaaacaaca gcttttgcac gttttcgcct ggattaacgg ttagctcctg
    25621 aggcgcgcca tttaaagtaa agtggatgat catacggctt cctccccagc ctgttggcag
    25681 tcggcataca ggtcggctac cagaactccc gtgatatagc gtttataggc cacgctgccc
    25741 cgcaaatctt cctgcgggaa aatggcgttg gcgacagcct gttcaagtgc attgccttcc
    25801 agattttgtt tttcgacatc gtgcagacgc agtgctttac tggcgacgcc atccagcgca
    25861 attcgcatac cgtcgtggtc tgtcattgca acggctgccg tcacgacggt taaacctgcc
    25921 tgagaacggc taattttgcg ggtcgcacag gtgcgatacg gatctttaat gataatttcg
    25981 gttagcaggc gatcgcatgg gcaggccagg tagtcctcga ttgacagcgt ttcgccgttg
    26041 ccaaaaacca gttcagcatc cagtgctagc aggacgggaa gcagcactga ctcttcctgc
    26101 cgggcggcga tttcaccacc aatggtcgac tgattacgaa catggcgtga gtaaacaaaa
    26161 ccgagggctt cacgcagtgc tgcaggaata aagcgcgcat cacgcagtgg ctgcaagcga
    26221 gacattgcac caatccgcag tgcaccgtta tcccagtcaa cccagtccag ttccagatcc
    26281 tgtaaggaaa tggcaatctt tttatcggta cgggttggtg tagcattgag tttgctgccc
    26341 ccggcgaacc agacggcttc atcctggtag cggcgcttca gttccagcgc ctgttcgaca
    26401 gagtcgggcc tgaaaaattg ttcaatcatc ttctttcctt atttaaccct cttccctgcg
    26461 ggaagagggc atctgtcatt tatgccagcg catccatccg acgccacata ctggcagcgg
    26521 cttttctggc ttgtgcataa atggaatcgc aatcgaagtt aaactgacgg tcttcataga
    26581 ccatcacacc attgaccatc acgctgtgaa cgctgcctga gcccataccg aaagcgatat
    26641 gcccggcgat attgtctgcc agcagcggcg tcggcgagtt gtaatcgcaa atggtcaaat
    26701 cagctttgta accggcttcc agaagcccaa atttcgcgcc aaagttgcgg ctcatcagtt
    26761 cgttaccgtt agtcagggct ttggcaaaac tgtcaggcca cagcggacca cccgcatcgc
    26821 gatgtttaaa gaaggcaaat ttcatctctt caaacatgtc cgaaccaatg ccgtccgttc
    26881 ccaatgccag gttgcggatg tcgctaaggt gatggttgta gccgacatgg ttgttcatgt
    26941 ttgaacgggc gttatgcacc aggaacgcat cgcgctgatt gagtagggcg atgtcatctt
    27001 tcgacaagta cagcccatga gcgaccagcg ttttgctgtc gatgaggtcg aattgcgcca
    27061 gtcgtgccag caggtctttg ccgtaccagt ggtgactgta ggaaacgtcg taaaggtctt
    27121 ccgcagcgtg aatatgcaaa ccacggcctg tggctttcac ggcttcacgc agcatctcca
    27181 gaccggcatc cggcacggta aacggcgcgt gagcaccgat atgtgcttcc accagatacg
    27241 gctcgcttgt cgctttcttc gcctcatcaa tcaaacgggc gaaacggatg ttttcttcta
    27301 caccttcctg caactctttg atgccgttgt tacggtcagt agtttcaaaa caggtcatcg
    27361 cgcgcaggcc aacttttaaa aatgcgtcgc gcaatgtgga gagcgacccg ccgatatacg
    27421 ccggcgaggc atggtgatcg ataaccgatg tacatccgct cttaatcgct tccagggaac
    27481 aaatcagtcc gctgtaatag agcgactctt catcaagggc gcgatcgagc cgccaccaga
    27541 gatttttcag cgttgagatg aaatccgggc aaggggcgat gtttgccata attccgcggg
    27601 aaagctccga gtaaaaatgg ttgtgcgagc agacaattcc cggcatcaca atccgaccat
    27661 gcatctcttt gtagctggca tcggggtagc gttgcgtcag ggcatcgccg atagcgacaa
    27721 tcacgtcatt ttcgatagcg atatcaacgc cttcctgcac tttcgccggg tgtaactgca
    27781 ctgcggtgac attcttcaga atcaacatga ttacacctcc acgcggccca gcagataatg
    27841 atgatgctgg tggacatggc tgatgatgcg gcacatatcg ttcagctccg gtggtacgtt
    27901 gttaaactga ccttcgctgt cgatgtttaa cacccagctt tggttattca gacgtactcg
    27961 tacccggcaa tcttccacaa ggaagcctgg ggtgctgctg ttatcaaagt cttgcgccag
    28021 gctgaagacg gtgattttgt cttcgtatgg tttaccgttc cacggacaga actgagcgca
    28081 gttgccgcat tcgttacagt aagcgtcgag gtgcagcgtc tggaatctat tctggaagcc
    28141 agggactgca atggagacgt tggcgcggtt cgggcagaca tccacacact tgttgcaaac
    28201 gtagttacat tcgaggcagc gagcggcttc ctgggcgaca aacgcgtcac gatcgtcact
    28261 gttcaccagc gtgatagaga tatcgccttt acgttgatag atttccgctg gattgacgtt
    28321 gttccagtat ttatcgttct ggtgggaacg gatattttcc cgactaagga tggcatcggt
    28381 cgcccgacgc gcggttccga cagcagcgac aatggaggat ggtccgcgct gtacgtcgcc
    28441 gatcataaag acgtcagtca gacgagtttc gccattatgg tcgacgtctg gccagccgtt
    28501 tttgtccagc ggcacgccca tcgcattcag ggcttcagta tcctgctgtt caccaatggc
    28561 ggtgatcagg ctgtctacat gcagtgttac tgtttcgttg gtttcaaccg gacggcgacg
    28621 acctttctca tccggttcgc caagcgacat aacgcgcaag gttaaggtgc catcagcatc
    28681 gaaacgttcc ggattattca ggaaacggaa ctctacgccg tcgtgcaacg cttcttcata
    28741 ctcttcgcgc catgcgggca tctcttgtag tgaacgacgg taaacgatcg ttgctttttc
    28801 tacgcctgga acgcgtaacg ccgcacgagc gcagtccatt gcggtgttac ccgccccgac
    28861 aacgaccaca tgtttgccca gcttgagcgc tgtacccttg ttgtattcac gcaggaaggg
    28921 gagtgatttc cagacatttt ggttgtcgcc cgccagtttc acaccgctat ttttatcagt
    28981 gccggtggca atcagaacat agtggaagcc ctgatttttt aactgttcaa cggttaaatc
    29041 gggtgagcag ccatactcaa atttcacgcc gtgagcggca acaaaatcga tatcgtgctg
    29101 aattaactct gcaggaatac ggaactgagg aatgatattt ttcaccacgc cgccagcatt
    29161 ggcttcgcgt tcaaacagcg taaccggatg gcccgctctg gcaaggaagt aacctgctgc
    29221 cagacccgcc ggacctgcac caatcacggc aaccggatgg cgtgaaccag aaccggcggg
    29281 tttgtgccag cgttgcttat attcatccca acctttttcc agcgcgactt ttttcagttc
    29341 gcggatattc agcgcactgt cgtaatccag gcgggtacag ttgtattggc actggtgatc
    29401 gcaaatatga ccggtaatgg caggtagggc gttgcgttgg tagatgagtt ccagcgcgtc
    29461 ggcatagcgg tgttcgccaa gcagacggat gtattccgga atatcttgct tgatagcgca
    29521 ggcagtaaca cagggggcaa cgtagcaatc ggtcagcggc aggtcttctg ccacttcaat
    29581 acgctcttct ggcttccagt gtttctgggt gtattccatg gttaacgcgt ctgctgccag
    29641 tctgttcagt cgttcgacgt caacatggtc aagtccccag gcgtcggagc cttccagctc
    29701 gcgcatgcag gcacttaagc gcagatagcc gccaggtttc agcaggtcgg ttgccatagt
    29761 aatagggcga atacctgtat caaaaatatc gcggatagtc agctgactgg caccaccgga
    29821 ataagaaatg ggcagtttgc cgtcaaaggc gcgagagaga actgctgcaa cattgatgga
    29881 gagcgggaac agcgcacggc ctgacatata catctcttca ccaggcagtg cgcctttatt
    29941 gttgatggtg ccgagagtgt tagtcagttt tacgccaaag ccgagtgatt tttcttttgc
    30001 cagtaccatc aggcgctcca gcatttccag tgcttgcgtc agcttgaggt cgtgatcaaa
    30061 tgactcttct tttaagccta tgtaaccgaa accgcagaca tcgaggatct cacgaacacg
    30121 cgcgtacccc agtaaggtcg ggttaagttt gacaaaggtg ttgagccctt tttcttccag
    30181 catgtagcgg caaatggctt cgatttcatg cggaggacag ccgtgcatgg tggagagggt
    30241 gacgccctgc accatactgg tggggatgcg agcgggtaag gcttgcaagt tttcgcgttt
    30301 ttcctgcaat ccgtggcggg ctaaaaatgc gtcatcctgg agtaatttat tcagcgtatc
    30361 gcgatattga gcgaatttcg gatggtcaga tgcgtccatc atattgtcga tgaactgttg
    30421 catcggcggt tgcttaatac cttcgaggtt gtaaccgacg ctcatattaa agatgaacga
    30481 tttaccggaa tcagaaggct gtaacatcgc ttcgagaagg tgcagggcaa accaggcttt
    30541 gaggtattca tcccaggctt taagcaaggt aaattcggta gaccattcgg tgttaaagca
    30601 ttcgtcttcg gcatcaatac agggcttttc cagctccagg cggtcaagaa tttggacggt
    30661 ttttagttcg atgaagcgtc cgccagtcag ccaggaagtg acaatatttt gcgcgagctg
    30721 cgtgtgcggg ccagcggcag ggccgacggg agtggcacag gtttcaccga agacgctaac
    30781 agttttacct tttacaggtg agtaaaactg ttgctcggga ataccaaaga ttgagcgttg
    30841 ttgttggtat tcatcaaata tgcgcgtcaa aagttcctca aacggaatgg gacgcataat
    30901 atcccccata accctctcct tagcttatac agatatcatt gagtgaccgg tgttgagaaa
    30961 tccggtggtt tagggggata gagcaacaat cgcaccactc ttgccaggtt tggcgggaaa
    31021 tataaaaaaa tgtgaggtgg ctcgaaactt aaaggctttt tgtgaatgaa ctcacaggtg
    31081 ccaaatgaga attaaaatca ctcctgaaaa ttattattgt cagcccattt atcacttttt
    31141 ctcttttctc aaaatcagtg attccttatc attatgataa agagtgctgg agagattaaa
    31201 tatttgaacg caatcgttta actacatttt ctaaatttta gtataccttt tttgcgctgt
    31261 aataaagtcg tccggagtat caatatctaa aattatttct gcattttcta tttcgacgga
    31321 ataatgatct cccataagga gtgcctgacg catattggtg acattgggtc gctggattgc
    31381 ctgcatcagg catggttttg acactaaaat cggatggccg gggatgccat tatggagtgg
    31441 cagtattgcg ccatcatttc gtaacgacca gatttttcta aaaatatcga tggtgagggt
    31501 tggcatgtca ccgtgggtga gaaaacaatg ttctgtttgc actgcgggta ctgcggcctt
    31561 gacggatgtt agtaaaccct gcgcataatc tgggttgtga ataatagtga tattgctctg
    31621 gttcgcataa cgttcgtgca gttcattacc acgatagccg gtgactaaaa taattcggct
    31681 acaaaactgc aacgcatttt tgatacttgt atcaagaatt gttccctgtt cccaggggaa
    31741 catcattttc cattgcccca ttcttgatga taatccggca gcggtaatta tacagtcgat
    31801 ggctgacata gtggttccat tatttatagg ggctaataac gcctgataat tgcgttactt
    31861 cctgatggat taagttgggg ataatttatc ccagagaggt cataaagact cgtgaaaagt
    31921 atagttgacc catcggcatt agtcattgat ttaggtgcgc agaaacgtcc cactgtgatt
    31981 tctgttgttg gtgctggggg gaaaaccagc ttgctttttt ggctggcaga actgctccag
    32041 gctagcggca ggcgtgtgtt aatcacgacg acgacacata tgtttatgcc aacgtctcac
    32101 tggcccgtgg ttttctgtcg tgatcccgcc atgcttcctc atgcgtctct tacatccccc
    32161 atttcatttt gttttcacag ctggaaagcg aaccagggaa aagtgcaggg atttacgcca
    32221 gaagcgattg atgcactggt gcaacgacca gagtgtgacg taattctcat tgaagcagat
    32281 ggctcgcgtg gaatgccgtt aaaagcgcct gatgagcacg aaccttgcat acccaaaagc
    32341 agttgctgcg tgattgctgt gatgggagga catactttgg gcgcgaaagt gagcacagaa
    32401 aatgtccatc gctggtcgca gtttgctgat attaccgggt taacacctga tgcaaccttg
    32461 caactgagcg atctcgttgc gctggttcgc caccctcagg gggcgtttaa aaacgtaccg
    32521 caaggttgtc ggcgagtctg gttcattaac cgtttttctc aatgtgagaa tgcgattgca
    32581 caaagcgagc tccttcaacc gctgcaacaa cacgacgtag aggcaatctg gctgggcgat
    32641 atacaagaac atcctgcaat cgcgcgcaga tttgtgaatt agcggcccgg gtattccggg
    32701 ttactgaaag gatatccatt gaggttggta tgaatatttt cacagaggct gcaaaactcg
    32761 aagagcaaaa ttgtccgttt gcgatggcgc aaattgttga tagccgaggc tcgactcccc
    32821 gccattctgc acaaatgtta gtgcgcgccg atggttctat cgtcggtaca attggtggcg
    32881 gaatggttga gtggaaggtg attgaagagt cacttcaggc attgcaggaa cgtaagccgc
    32941 gattattcca tggacgtatg gctcgtaacg gtgcggatgc tgtcgggtca gattgtggag
    33001 gtgcaatgtc agtatttatc agcgtccatg gtatgcgtcc acgtctggtg ttgattggcg
    33061 cggggcatgt caaccgggcg atagcccaga gtgcggcgct attaggattt gatattgccg
    33121 ttgccgatat ttatcgcgaa agcctcaatc ctgaactatt cccaccatca accacgcttc
    33181 tccatgctga gtcgtttggt gcggcagtgg aagaactgga tattcgccct gataattttg
    33241 tcctgattgc cacgaataat caggatcgtg aagccctcga caaactcatt gaacagccca
    33301 ttgcatggtt ggggttgctg gcaagtcgcc gcaaggttca gcttttcctg cgtcaattgc
    33361 gtgagaaagg cgtggctgaa gaacatattg cccgtttaca tgcgcccgtt ggttacaaca
    33421 taggtgcgga aacgccgcag gagatcgcca tcagcgtgct ggcagaaata ttacgggtga
    33481 aaaataacgc gccgggtggg ctgatgatga aaccttctca tccttccgga caccagctgg
    33541 tggtgattcg cggtgcggga gatatcgcca gtggtgtggc gctacgtctg tatcatgcgg
    33601 gttttaaagt gatcatgctg gaagtggaaa aaccgacagt gattcgttgt accgtggcgt
    33661 ttgcccaggc cgtgttcgat ggcgaaatga cggtcgaagg cgtcactgct cgcctggcaa
    33721 ccagctctgc ggaagcgatg aaacttaccg aacgcggatt catccctgtg atggtagatc
    33781 ccgcctgttc attgcttgat gaactgaaac cgctttgcgt ggtggacgct attctggcga
    33841 aacagaattt gggaacgcgg gcagatatgg caccagtaac aatcgcgctt gggccgggct
    33901 ttactgcagg gaaggattgt catgcggtaa ttgaaacaaa tcgcgggcac tggctcggtc
    33961 aggtgattta ctctggttgt gcgcaggaga ataccgatgt tcctggcaat attatggggc
    34021 ataccacccg acgggtgatc cgtgctcctg ctgcaggcat tatgcgatcc aacgtgaaat
    34081 taggcgatct ggtgaaagag ggcgatgtga ttgcctggat tggtgagcat gaaattaaag
    34141 caccgttgac ggggatggtg cgtggcttgt tgaacgacgg cctggcagtg gttggtggtt
    34201 ttaaaattgg tgatatcgat cctcgtggtg aaaccgctga tttcaccagc gtttctgata
    34261 aagcccgggc gattggcggc ggcgtacttg aggcgttaat gatgttgatg catcagggcg
    34321 tgaaagcgac aaaagaagtg ctggaagtgg cttaaatgaa aatggccgga tggtattacc
    34381 cacccggcct taaaattact gacaaatcac cgttccggtt ttgccttcaa tcccttcttt
    34441 cgctttgctc aatacggtaa tcagtgcttc acgacctgcg cgagagcgag caaacgaggc
    34501 ggcagcttcc actttaggca gcatagaacc tttcgcaaaa tgaccttctt cgataaagcg
    34561 ttcagcatca cttaatgaca ggcgatcgag ccactgttca ttctctttac cgaagttaat
    34621 ggccactttt tctaccgcag tcagaatgat cagcatatcg gcatcaatca tttctgctaa
    34681 acgggcgctg gcccagtctt tatcgataac cgcgctggca ccacgcagat ggttaccttc
    34741 acgaataaca gggataccac caccgccaac ggcgatcacc acctggcctg catctaccag
    34801 agctttaacc gtttcttttt caatgatatc aaccggtttt ggcgaggcaa caacacggcg
    34861 atagccgcga ccagcatctt ctttcagagt gtaaccctgt tttgtcagtt gctctgcttc
    34921 ttgctcggta aagaacgaac cgatcggctt ggtcgggttg aggaatgccg gatcgttagc
    34981 gtcgacttcg acctgagtaa ccagcgttgc tacaggttta ttaatgcccc gagaaagcag
    35041 ttcttctctt aacgcgtttt gtagatcgta accaatataa ccctggctta aggcaacaca
    35101 gacagacatc ggcagcattg gcgagtgtgc ttcagttttt gcggcagctt caaaggcttg
    35161 gttaatcatg cccacctgcg gcccgttacc atgagtgacg ataacttcat gtccttgggc
    35221 aattaaatca acaattgcct gagaagtaat ttttaccgct ttcatttgtc cggccaggtc
    35281 atcgcccagc gcattcccgc ccagggcgag aacaattttc ttactcattt ttcctcactc
    35341 atcattcttg attgtgtata gatccctgac agtggttaga gcacgggagg gacaaacggt
    35401 ttgcgtcgca ggaaacgacc tcgcccggct ttgcctgtaa aggtgccgtc acagaaaatc
    35461 gtttcaccac gggataaggt tctgacaatc gcgccctgac aggtaaaacc ctcccatggc
    35521 gagtagtcgg cgttgtcgtg gagatggcga tgctgaattt gttggctctg acgtgggtcg
    35581 ataatcacca cgtcgccatc ggaaccgggc gctaataatc ctttttgcgg ccacaggcca
    35641 aacagcctgg cgggcattgc gctggttaat tcaacaaagc gttccggtgt tatacgtccc
    35701 gtcatcacgc cactggaaaa cagtaactgc atgcggtttt ccacaccggg taagccattt
    35761 gggcagcgac tgaaatcgcc tttagaaatt tgcaggcgtt gagccatcga gaaggtgcag
    35821 tgatcggtcg ccaccacgtc aatcgcacca tcgctgatgc cacaccacag tttgtcctgc
    35881 tcgcgtacgt tacgcagcgg tgggctaaga atgaacttca tgccatcttc tgtatcgtaa
    35941 ctgcgttcgt ccaacaggag atattgtggg caggtttcaa cccagactgg ctggtgattc
    36001 gcacgggcaa gacgcagata atccagacct aagccgttag acaggtgcac gatatagagc
    36061 ggggcgttac cggcaatttg tgccaggtta atcatgcggg cgatggcttc cgcttcgcat
    36121 tccagagggc gactcaaggc atgatagcgc ggcgcggtta accctgcggc gataaattcc
    36181 gcccgcttgc tggcgatagc cgcatcattt tccgggtgca cggtggtcag cgcgccggat
    36241 tcatgcaagc ggcgtaatgc ctgcaaaacc tcgtcatcgt tgagtttgta ttgataggtt
    36301 aaatagagtt taaaactgct cagtccttcc tcgaccatca tcgggatttc gtcgaggatt
    36361 gcgtgattaa tgtgctggat cacaccgtga aagctgtaat cgatgaccgc tttatgggcg
    36421 gcataaccac gataaacctc cagttgatgg cgtaaccgac agccgtttgg gccaaatccc
    36481 atatggtcaa taatggttgt tgtaccgcca cacgcagctg cgcgggtacc ggtaaaaaaa
    36541 tcatcacaac tgcgcgcgat gccgacatca atattgaaat gcgtatggac atccacgccg
    36601 ccagggaaaa cgtaacagcc agtggcatca atttcttcat acgggagctg cggcgaaata
    36661 ttgttgccca actggcgaac aatcccgctt tcaatcagca aatcctgttt ggcttgtcca
    36721 tctgcgttaa cgacagtgcc gtttttgatc aatacgcgca tagcaaactc caaaaaccct
    36781 ccggcgaacc ggaggggaat aagtgaagag taattactct gttgccagcc agcttaacgg
    36841 gattgcagcg tacatcgcgg cacaggtcac caggtgagat ttccaggttt tttcgttagg
    36901 tgcatgcgct tcaggttctt tacccgggcc aaagccgatc accggaatgc cgtgacggcc
    36961 catgatagat acgccgttag ttgagaaggt ccacttatca acaaccggcg ctttgccaaa
    37021 cagcccttcg taggcattca ccagtgcttt aacggtgaag tgatcttctt ccactttcca
    37081 ggtcgggaag tagcattcgg ttgggtaaac caggccagtc caggacggac ggtcgtagtt
    37141 gtacatagaa acaaccgcgt tagctttctg tactgcaggc agggcgcgga tttcgtccag
    37201 cgcgccttcc caggtttcgc cccaggtcag acggcggtca atagagacgg cgcagctatc
    37261 tgctacagcg caacggcttg gggatgtgaa gaagatttca gaaacggtga gggtgccttt
    37321 gccgaggaat tcgtcataac ccagacgttg ggagagttct tgtaattcgc caagaatcgg
    37381 acccattttg aaaatggcgt tgtcaccgcg ttctggcgca gaaccgtggc agctaacacc
    37441 ctgaacatca atacgaattt ccatacgacc gcgctgacca cggtatatct ggcagtcggt
    37501 tggttcggta ctgaccacaa attccgggcg aatgccggat tgttcaataa tgtactgcca
    37561 gcacagaccg tcgcagtctt cttcctgcac agtaccggta accagcaggg tatattcatc
    37621 ttccagaccg aggtctttaa tgattttacc ggcataaacc atagatgcca tgccgccttc
    37681 ctggtcggaa gtaccgcgac caccgattag ctcatcagtt tccatgcctt cgtacggatc
    37741 gaagtcccag tttttgatgt tgccaatgcc gacggtatcg atatgagcgt ccattgccac
    37801 cagacgcggg ccgtggccga tataaccgag aacgttgccc atcgggtcga tttcaacttt
    37861 atcgaagccg actttttcca tctcttcttt aatacgatgt actacgcgtt tttcgtcgca
    37921 gctttcactg ggaatagcaa ccatgtcgcg caggaagcga gtcatgtccg cctggtaatc
    37981 ttttgctttt tcaagaatca gtttgaatgg aatattctta gccattattt ttctccagat
    38041 tttttactcc ccgttttgca gggagtagtt ccaatagggt gattaaggtg ctacagcgtg
    38101 tttgccttcc cagacaactt cgcggtagtg cttcacgtcg gtgtcgcctt cagtgctgat
    38161 aaccagcact acggcatctt tgtccagcgc cagtttttcc atcaggcttt gatgttgcgg
    38221 gtgataatga accgctgcga gaacgcccaa accgacagcg ccggattcac cggagatgat
    38281 gcgcgggtcg ttgccgtacg gattacccag cacgcgcata cctaatgcgg caacgctgtc
    38341 ctggcaggag atgaattggg tggcgcagtt acgtaggatt tcccagccca gcgggttagg
    38401 ttcgccacag gccaggcccg ccatgatggt ggccatatca ccgccaacgt tgacgatgtc
    38461 gcctttgacg ccggagcgat aaatacagtc agctttgtca ggttcaacaa taatgctgtg
    38521 cagattttgc gggctgtaga cgtcgaccag ataacccagc acaccaccgg ccattgctcc
    38581 gacaccggct tgcagcagaa cgtgcgtcgg ggttacgccc atttcacgca tttgctcgac
    38641 ggcttcatct gccagggttg cgtagccttg catgatccag gttgggattt tggtgtaacc
    38701 ttcccatgcc gtgtcctgta ccacttccca gccgtgctgc tgcgcgtgtt gcatggtcag
    38761 gcgaacggta tcgtcatagt tcatatccgt gacgatgcac tcggcaccga ggttcagaat
    38821 ggcgtcaacg cgttcctgag cagaaccttt cggcatgtaa atcaccgcat tctgtccgag
    38881 ttgctgtgct gcccacgcca caccgcgccc gtggttgccg tcggtggtgg tcgcgaaagt
    38941 cattttttcg ccgatggcat ttttcaggtg ctcaaatgac agcgtttcga tatcaagatg
    39001 atatttttca cacaataatt gagcgatggc gtacgcaccg ccaagcattt tgaaggcgtt
    39061 cagaccgaat cgttttgatt cgtctttgac gagaattttc ttcacaccaa aaaggtttgc
    39121 gagatcgtcc agcgcacaaa gcggtgttgg gcgataacca gctattttct ggtggaactg
    39181 gcgcgccagt ttggcctggc tttgcgaaaa gagcggtgat gtttcgccgt tgaaaaattt
    39241 gttatcggcg atatcaatct tcaatgagaa aacggacata tcctatcctt ttggcctctg
    39301 gaaatagagg gccgaccagc agatcggccc gaggggttat ttcacgcgtt cttgcgcttc
    39361 tttcagcagt tgctcgagca gtgcacctgg tttggcgtat ttacgggaca ggatcatcgc
    39421 ggcgatgata taaggcttcc agctggcttc tttgtaggta gcgatacggt atttttcgaa
    39481 tacgccttca gtcacttcac cttctttgca ggatacgccg ctgatatcag ctggcaggca
    39541 gtgcatgtac agggcttcgc catcacgggt cagttccatc atctcttcag tacaatgcca
    39601 gtctttgtgt tgcgcgttct gtgccagaca ctgtttttcc agtgctttta agccttcgtg
    39661 gtcgttcgca cgcagcaatt cagtacgctc ttccatcact ttgtaaggtg cccatgactt
    39721 cggataaacg atgtctgcgt ctttgaaggc ttcttccatg ctggtgacct gacggaagct
    39781 accaccggag gctttagcat tgtttttcgc cacttcaacc acatccggga tcaggtcgta
    39841 gccttccgga tgggccaagg tgacatccat accgaagcga gtcatcagac cgatgatgcc
    39901 ttgtggtaca gagagcggtt tgccatagct tggagagtag gcccaggtca tggcgatttt
    39961 tttacctttc aggttttcca gtgaaccaaa gtgttcacgt aaccacgcga ggtcagccat
    40021 tgactgagtc gggtggtcaa tatcgcattg caggttcact aaagccggac gctgtggcag
    40081 tacaccctgc ttgtaaccgt catcaagtgc agcgccaact tcacgcatat aggcgttgcc
    40141 tgcgcccaga tacatatcgt cgcgaatacc aatagcgtcg gcgcagaagg agatcatatt
    40201 ggcggtttca cgcacggttt cgccgtgagc gatttgtgat ttgccttcgt cgagatcttg
    40261 ttgtgcaagg ccgagcaggt taagcgcgga agcataagag aagcgggtac gggtggagtt
    40321 gtcgcggaat acggaaatac ctaatccact attaaagact ttggttgaga tgttttcagc
    40381 acgcagtgct tttaatgctg cggcaacgtc cagtacttgt ttcagttcat ctggcgtctg
    40441 ttcccacgtt aacaaaaaat ctttctcgtg aaggtgagag gtcagcgaat tgatatcctt
    40501 aatcagctca ttaacagttt tcatcataca gtcccggtag tggaatgaca ggcaaagtgg
    40561 tttatttcac tgtgacttgc caaagtgatt ggtgttttca tagctatacc tgggactaac
    40621 aataagtgtg ccagaattat ccgcgcggct gcaaaagact aatttgacgg ataatgtgtg
    40681 gggttgttca caaattgatg ccagcttctg gttgtttgta aaatacgaaa acgtttggct
    40741 ttttttatcg cgttatttat ttgctgctaa gtatcagaaa taagataaag gggcaggcta
    40801 tctctcttca gtttgataaa ctcaccgccg taattctcat attgatatag aaatgtgata
    40861 tctcttccat aattgcatta gaaaatgata aaaatcttat gtttttatca aagccaattt
    40921 cgcctgagtt aatatcatca tgatctgtga accatcaacg tcttcaatga tcgactttta
    40981 agtaagtctg gaaggtaaag ctatggagct tgctactacg cagtcagtat tgatgcaaat
    41041 tcaaccgaca attcagcgtt ttgccagaat gcttgccagc gttttgcagt ttgaggttga
    41101 gatcgttgat gaaaacttgt gtcgcgtcgc cggaacgggc gcgtatggga agtttcttgg
    41161 tcgccagttg agcggcaact cacgcctgct ccgccacgtc ctggaaacga aaactgaaaa
    41221 agttgtgaca cagtctcgct tcgatcccct ttgcgaaggt tgcgatagta aagaaaattg
    41281 ccgcgaaaaa gcatttctgg gtacgcctgt cattttacag gatcgttgtg ttggggtgat
    41341 aagtttgatt gccgttaccc acgagcaaca agagcatatc agtgataatt tacgcgaatt
    41401 ttctgattat gttcgccata tatccaccat ttttgtttcg aaacttctgg aggatcaggg
    41461 gccaggagat aacatcagta aaatattcgc gaccatgatc gataatatgg atcagggcgt
    41521 attagttgtt gatgatgaaa gtcgggttca gtttgttaat cagactgcct taaaaacact
    41581 tggtgttgta caaaataata ttattgggaa acctatccgt ttcagaccat taacatttga
    41641 gagtaatttt actcatggac atatgcagca tattgtttcg tgggacgata aaagtgaatt
    41701 aatcattggt caattgcata acattcaggg ccgacaatta tttttaatgg catttcacca
    41761 atcgcatacc agtttttctg tagcaaatgc acctgatgaa ccacatattg aacaattggt
    41821 tggcgagtgc cgtgttatgc ggcaattaaa acgactcatt agccgtattg cacccagccc
    41881 atccagcgtt atggtggttg gtgaaagcgg cacgggtaaa gaagtcgtcg cccgagcaat
    41941 ccataagttg agcggaagac ggaataaacc ctttattgct atcaactgtg ccgcgattcc
    42001 ggagcagctt ctggaaagcg aactgttcgg ttatgttaaa ggcgcattta ctagtgcttc
    42061 tgccaacggt aaaacagggt tgattcaggc ggcgaatacg ggcacgctgt ttctcgatga
    42121 aataggtgat atgccattaa tgttgcaggc taaattactg cgcgctattg aggcgcgtga
    42181 aattctgccg attggtgcca gtagcccaat acaagtcgac attcgcatca tttctgcaac
    42241 taatcagaat ttggcccagt tcattgccga aggtaaattc cgcgaagatc tcttctaccg
    42301 acttaatgtt atcccgataa ctctgccacc gctgcgtgaa cgtcaggaag atattgaact
    42361 attggtgcat tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat
    42421 tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt
    42481 aagcaatttg atggaatatc tggttaacgt ggttccttca ggtgaagtta tcgacagcac
    42541 gctattgccg ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggt
    42601 cagtgaggcg cacctgtcac tcgatgatgc gggcggcacg gcgctggagg agatggaaaa
    42661 gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag aagcaagttg ctgatgaact
    42721 gggcatcggc attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata
    42781 acctcagtga atccggcctg aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat
    42841 ccagaactgt atttacaatc atctgatacc ccgtgcagcg acaaagattt cccgccagtc
    42901 cgcgacgaat ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc attgccgtgg
    42961 tagccataat caggccaggc gtacaaaacc cgcactgcac tgcgccggat ttcgcataag
    43021 cctgctgaac atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg cggatctcct
    43081 ttccttcagc ccaggcggca aggtataagc aactgtctat tgctgtgccg tcgaccaaca
    43141 ccgtacaggc accacattca cccacgcagc acccttgttt gacacttagc agtccttgtt
    43201 cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt
    43261 taatggtgca ttcgatggta attgtttcgc tgtgattcat tgcaattttc cccccgccgc
    43321 ggcgacggct tcgctaatca cttttttggt catcgtctgg atgagatgca gacgaaactc
    43381 tttactggcc cgccatgaag aacgcggggc gacatcttgc aggacagatt cgctgatagc
    43441 ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg
    43501 aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat tgccgttatc
    43561 cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc gcatggcata
    43621 tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag
    43681 gatttcgtca tgctcaagag acactttgcc cggcccggtg tgaaagccat taatcgggac
    43741 gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg tcataaatta gcgttggcgt
    43801 ggcagaatct gcgctggtgg caccgttgca aatatttcca ccgtaggtag cgacgttacg
    43861 gatctgcggt ccagcaatgg acgtggccgc agcacataac gccgggagat gacgttgagt
    43921 tataggagct tctattagct gggtaaatgt cgttgcagag ccgatacgta gcgagccatc
    43981 ttccgccagc gtaattcccc gcagctccgc cagattatga atatcaacaa tatggcgata
    44041 acggtcattg tgatggtgga gctgaatcag tacgtcagtg ccatcggcga gcagtttggc
    44101 ctgcgggttg tcagccagca ggttgatggc atcggcaagg gttgctgcgc gatggtaaga
    44161 agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc cagatggaac tcttcatata
    44221 accgttttgg cgtcagcggc agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt
    44281 tacgaatagc agcggcaaca ggaattattg gtggctcacc cagtgactta tgtccgtatg
    44341 cggattgcgg ctcattgatt tcgacgaacg cgctttccag ttgtggcaga tccggcatgg
    44401 tcggcatttt gtaatccagc agattggggt tacggaccac gccgctttta gcatcgatga
    44461 tcatctcttc aaatagcgcc cagccaatgc ccattcccat tccgccgtgt acctgacctt
    44521 ctgccagcag tggattaaga atatgccctg aatcatgaac gttgaggatg cggttgatgg
    44581 tgactttgca cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca aacgccgatg
    44641 ggttagtggt ggttttgatg gagctttcag cagagagctg cccgccgcgt tcagggtggt
    44701 agaaagcgtc catcgccaaa tcttttaacg acattaacgg ctcttccggt cgttcaacca
    44761 gcacgatatg gccttttatc agggtcagat tcatcgctga ctgatgtagc atgactgcgg
    44821 cgtgagcgat gattttctct tttaataata gtgccgcact gcgcagcgca ggcgcggcaa
    44881 catagctctg gcgtgaggca aatgcgccgg gagcgaacgg cgtaacgtcg gtatcttgag
    44941 ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc atttgcgaga
    45001 agacggtgtc ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg atggttccat
    45061 cctgattcat cagaaggcgc gcgcctgcta tttctacgcc gacaggccag gtgttagagg
    45121 tatagctaaa acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt
    45181 ctgcacggcg tttttcccat tcaaagattt tccggccttt ttcaagacac tccggcaacc
    45241 ctgcgctgta aatacgtttg cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc
    45301 gtaaacgaat ttcaacagga tcaataccta acgctgtcgc ggcgtcatca agcatagact
    45361 caacggcaaa tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt
    45421 tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg taagcgactt
    45481 tattcctccc cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg gtgttagaca
    45541 gaacatccag actataacct ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa
    45601 tggtaaaagc gtggcgggta cgggttgcga ggaaacactc ttcacggcta agggaaactt
    45661 tcaccggaat gccgccaagc ttgctggtca ggaatgctgc cattggctct tccagtacat
    45721 cctgtttatt accaaaaccg ccaccgacaa acggtttgat gactcgtacg catgaccagg
    45781 gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg gtgctggaaa
    45841 cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt tccatgtgac
    45901 aatgttgaat aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg
    45961 tttgttggac attacccgtc gacatcgtgc tttgtttcag taaattgcca ccgttatgga
    46021 ttggtgcagc gtcttctgcc agcgccgctt ctggcgtggt gataacgggt aattcttgcc
    46081 actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga
    46141 tggcaacggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga tcggcggtat
    46201 cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga atatcaggca
    46261 catcttccca ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt tcatcattaa
    46321 tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcataa cacatgcccg
    46381 ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg gcatcgacgc
    46441 gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt
    46501 gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg
    46561 acgataagcg ttaggggagg taggaagtgt gatgacgtta aaaaaacaca gatatgatac
    46621 gaaacaggac tctgaatagg ggcagtgata cgtatcaata tgagtggcga taaaatcaat
    46681 gtgataaatt gggattatta cgcatcagga tgggaaatag ggtaaaaagt caggaagttc
    46741 gatacctcta cagcgaatat tttgctgatg atacttccct ttgccaaatg aaattaacct
    46801 ctttctctat taccacgttt ttccagaagc aagagattgg gtatcaagag gctggctgct
    46861 atgataaggc gtcttgtttt ttaagcgagg aaagattttg agtgcgggac gcctgaataa
    46921 aaaatctctg ggtatcgtga tgttgttatc ggttggactg cttttggcgg gctgttcggg
    46981 tagcaaatca tccgatacag gaacgtattc cggctccgtt tacaccgtga aacgggggga
    47041 tacgctatat cgtatttcgc gcaccacggg aaccagcgta aaagaactgg cgcgactgaa
    47101 cggcatttcc cccccttaca ccattgaagt tggtcagaaa ctaaaactgg gtggggcgaa
    47161 aagtagcagt agtacacgta aatcaaccgc caaatcaacg accaaaaccg catcggttac
    47221 accgtcatca gcggtaccga aatcatcctg gccgccagta gggcaacgtt gttggttatg
    47281 gccaacgaca gggaaagtta tcatgccgta ttcgacagca gatggcggca ataaagggat
    47341 tgatatctca gctccacggg gtacacctat ttacgccgcg ggtgcaggaa aggtggtgta
    47401 tgtgggcaac cagctgcgtg gctacggtaa tctcatcatg attaaacaca gtgaagatta
    47461 cattacggct tacgcccata atgacacgat gctggtaaat aatgggcaaa gcgtgaaggc
    47521 tgggcaaaaa atcgccacta tggggagcac ggatgcggca tctgttcgcc tgcatttcca
    47581 gattcgttac cgtgcaacgg caattgatcc gctacgttac ttgccgccgc agggcagcaa
    47641 gccaaaatgc tgatggcgaa ttaatcagca gtcagcagcg cggcacttgc taaggagagc
    47701 gtaaggttta taatgcctta cgcatctcga agcgggcgta gttcaatggt agaacgagag
    47761 cttcccaagc tctatacgag ggttcgattc ccttcgccgc ttttgctgta tctgattttc
    47821 tatattatct taattcaatc gcttcagact tttcgcaatc gccgtaatat ttccccattt
    47881 tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg taacaaccca ggtaaaacac
    47941 cttattttca agttgttaaa taagtggtta tttacgcttt taacaaacga ttgatggaat
    48001 attttatgaa agggaaatct gcattaactc ttctgctcgc tggtatattt tcgtgcggca
    48061 catgccaggc gacaggggca gaagtgacga gtgaatctgt atttaacatt cttaattcca
    48121 cgggggcagc aacagataaa agttatttat cgttaaatcc agataagtac cctaattatc
    48181 gtcttctgat tcactcagcc aaattaaaaa atgaaataaa gagccactat accaaagacg
    48241 aaattcaagg gttgttaaca ttaacagaaa acacacgaaa gttaacgttg acggagaaac
    48301 cctggggaac atttattctt gcttctactt tcgaagatga caaaacagcg gcagaaaccc
    48361 actatgatgc tgtctggctg agggatagtt tatggggcta tatggcatta gtatcagatc
    48421 agggaaatag tgtagccgca aaaaaggttc tgctcaccct gtgggattat atgtctacgc
    48481 tcgaccagat taagcggatg caggacgtca ttagcaaccc caaacgactg gatggagttc
    48541 ccgggcaaat gaatgctgtg catatccgat ttgacagcaa ctcccccgtg atggcagatg
    48601 tgcaggaaga aggcaagccg cagctgtgga accataaaca aaatgatgcg ttaggtcttt
    48661 atcttgatct ccttatacaa gcaatcgaca ctggtacaat taacgctgaa gattggcaaa
    48721 aaggtgaccg attaaaatcg gttgcgctgc tgattgctta tctggataaa gccaatttct
    48781 atgttatgga agattccgga gcatgggaag aagatgcccg attaaacaca tcttccgttg
    48841 cactggtgac ctctggactg gaacgtttgt cgaacttact ctcgaagaaa gactctgttt
    48901 ttgtctctga tttactacgc gaagctaaag ccaatgaact ggatgaacct ctttcaacaa
    48961 cgaggttgaa ccacctcata gataaaggtt atgaaagaat aacgttacag cttgatttag
    49021 ggggagaatc cccaggttat ctggaaaaag ataaacatta cagagaagcc gatgcggctt
    49081 tacttaatgt tatttatcct gcaaatcttg caaagataaa caccagaaga aaagagcagg
    49141 ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat aaaaagatac gagaaggata
    49201 attatcagtc ggcaaatttt tggttcaatg atattaaaac cgataccgat caaaattcac
    49261 atgcgaaaag a
//
LOCUS       DAZPJP010000008        46693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPJP010000008 DAZPJP010000000
VERSION     DAZPJP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.008"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHU9J_000417"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHU9J_000417"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1904064.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHU9J_000418"
     CDS             973..1341
                     /locus_tag="ACHU9J_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1904065.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHU9J_000419"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHU9J_000419"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1904066.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHU9J_000420"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHU9J_000420"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1904067.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHU9J_000421"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHU9J_000421"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1904068.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHU9J_000422"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHU9J_000422"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1904069.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHU9J_000423"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHU9J_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1904070.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHU9J_000424"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHU9J_000424"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1904071.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHU9J_000425"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHU9J_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1904072.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHU9J_000426"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHU9J_000426"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1904073.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHU9J_000427"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHU9J_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHU9J_000428"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHU9J_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR1904074.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHU9J_000429"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHU9J_000429"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1904075.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHU9J_000430"
     CDS             complement(12998..13573)
                     /locus_tag="ACHU9J_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1904076.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHU9J_000431"
     CDS             complement(13634..14083)
                     /locus_tag="ACHU9J_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1904077.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHU9J_000432"
     CDS             complement(14070..14495)
                     /locus_tag="ACHU9J_000432"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1904078.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHU9J_000433"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHU9J_000433"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1904079.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHU9J_000434"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHU9J_000434"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1904080.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHU9J_000435"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHU9J_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1904081.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHU9J_000436"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHU9J_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1904082.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHU9J_000437"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHU9J_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1904083.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHU9J_000438"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHU9J_000438"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1904084.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHU9J_000439"
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHU9J_000439"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR1904085.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHU9J_000440"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHU9J_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1904086.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHU9J_000441"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHU9J_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR1904087.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKTGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHU9J_000442"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHU9J_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR1904088.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHU9J_000443"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHU9J_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1904089.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHU9J_000444"
     CDS             complement(26015..27418)
                     /locus_tag="ACHU9J_000444"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1904090.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHU9J_000445"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHU9J_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1904091.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHU9J_000446"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHU9J_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1904092.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHU9J_000447"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHU9J_000447"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1904093.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHU9J_000448"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHU9J_000448"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1904094.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHU9J_000449"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHU9J_000449"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1904095.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHU9J_000450"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHU9J_000450"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1904096.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHU9J_000451"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHU9J_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1904097.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHU9J_000452"
     CDS             complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHU9J_000452"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1904098.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34334..35284
                     /gene="tal"
                     /locus_tag="ACHU9J_000453"
     CDS             34334..35284
                     /gene="tal"
                     /locus_tag="ACHU9J_000453"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1904099.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35304..37307
                     /gene="tkt"
                     /locus_tag="ACHU9J_000454"
     CDS             35304..37307
                     /gene="tkt"
                     /locus_tag="ACHU9J_000454"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1904100.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37403..38446)
                     /locus_tag="ACHU9J_000455"
     CDS             complement(37403..38446)
                     /locus_tag="ACHU9J_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1904101.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHU9J_000456"
                     /pseudo
     CDS             complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHU9J_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHU9J_000457"
     CDS             complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHU9J_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1904102.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41400..43100
                     /gene="narQ"
                     /locus_tag="ACHU9J_000458"
     CDS             41400..43100
                     /gene="narQ"
                     /locus_tag="ACHU9J_000458"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1904103.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43264..46377
                     /gene="acrD"
                     /locus_tag="ACHU9J_000459"
     CDS             43264..46377
                     /gene="acrD"
                     /locus_tag="ACHU9J_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1904104.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHU9J_000460"
     CDS             complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHU9J_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1904105.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10675 a        13144 c        12105 g        10769 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgagtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgccag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcgtca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctggtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accggtttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acgcagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt tattattcgc
    31681 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
    31741 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
    31801 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
    31861 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
    31921 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
    31981 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgcc gcatcgccgt
    32041 gcatttcacc atcaatcatc agttctggcg cacgttccct gaccagttcc agcgcctgac
    32101 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
    32161 cgcgcggttc aataccgaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
    32221 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
    32281 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
    32341 ttttcaccac gctaaaatgt tcatgataat cacccaccgt accgcaaatc attgcatcgg
    32401 cttcgccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
    32461 gcgcctgttc ctgagtgacg ccgcgacgct tcatgatctg gaagtattcg gtccagtact
    32521 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
    32581 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
    32641 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
    32701 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
    32761 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
    32821 gacgcgtcgc cacgcccgac tccatcgccg ctttcgcgac cgcaggagcg atcttaacga
    32881 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
    32941 cgccatacgc tgaagccacc acttcgctct gttccgcatg ggccagttct gcaatcgcgc
    33001 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
    33061 ggaagatgaa cgggaagcaa aggacgttgt tgacctggtt tggatagtca gaacgaccgg
    33121 tgcagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
    33181 gattcgccag cgccagaatc attggtgccc gagccatttt cttcaccatt tcctgggtca
    33241 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
    33301 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccatg tttggctcac
    33361 ggccttgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
    33421 gcgctaccag cagattcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
    33481 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
    33541 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
    33601 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
    33661 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
    33721 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
    33781 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
    33841 ccgccaccag gttaccgcgg gcggtatatt tgtaggcttt taacgggtct ttttcgattt
    33901 caagacaagg tgcggcaacg ccgggtgagt aggccagcgc cagatcgcgc tgcgttgcca
    33961 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
    34021 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
    34081 gtgtgtgggc aacaccccct gcccacaacg tggcgcacat tattaccttg ccggagtcta
    34141 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
    34201 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
    34261 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
    34321 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
    34381 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
    34441 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
    34501 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
    34561 ggcggtcaat ttcggtgctg aaatcctcaa aatcgtaccc ggtcgcgtgt caacagaagt
    34621 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
    34681 cttgtatcag caacaaggcg ttgagaaatc acgcattctg atcaagctgg cttcgacctg
    34741 ggaaggaatt cgcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct
    34801 gctgttttct tttgcacagg cacgggcctg tgcggaagca ggcgtttttc tgatttcgcc
    34861 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
    34921 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
    34981 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
    35041 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
    35101 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
    35161 cgaagcggag ttccgttggg agcacaatca gtatgcgatg gcggtagaaa aactgtctga
    35221 aggcattcgt ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
    35281 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
    35341 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
    35401 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaacct
    35461 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
    35521 tgctgcatct gaccggttac gacctgccgc tggaagagct gaagaacttt cgtcaactgc
    35581 attcgaaaac cccaggccac ccggagattg gttatacgcc aggcgttgaa accaccaccg
    35641 gtccacttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
    35701 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
    35761 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
    35821 gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
    35881 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
    35941 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
    36001 tgaaagataa gccgtcgctg attatctgtc gtacggtgat tggctttgga tcgccgaata
    36061 aagcaggtaa ggaagaggcg cacggcgcac cgctggggga agaagaagtg gcgctggcac
    36121 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagatatt tatcacgcct
    36181 gggatgcccg cgaaaaaggc gaaaaagcgc agcagaggtg gaatgagaag tttgccgcct
    36241 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg tcggatgagc ggtggtttac
    36301 cgaaggactg ggagaaaacg actcagaagt atatcaatga gttgcaggcg aatccggcga
    36361 aaatcgccac ccgtaaggct tcgcaaaata cgcttaacgc ttatggcccc ttgctaccgg
    36421 agctgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
    36481 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
    36541 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
    36601 tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
    36661 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
    36721 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
    36781 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
    36841 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
    36901 aagagattac tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
    36961 tgattgccac cggttcagag atggaaatca ccctgcaagc tgcggagaaa ttagcaggag
    37021 aaggtcgcaa tgttcgcgtg gtttccctgc cctcgaccga tattttcgac gcccaggatg
    37081 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
    37141 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
    37201 gttatgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
    37261 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatag tgatcgccgg
    37321 atgctgattg ccggatgcga cgctgacgcg tcttatccgg cctacagcgt cttatccggc
    37381 ctacatgtcc ccgccatttt gtttaacggg tgatccacaa cgtgggccag gcgtctggcc
    37441 catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt
    37501 caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca
    37561 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
    37621 gcccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg
    37681 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagctg atcatcatca
    37741 gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
    37801 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg
    37861 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
    37921 cgctgagcgg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
    37981 gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg gtctgattgc
    38041 cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg gtgatggttg
    38101 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca
    38161 acggctcacc atctaacagc agccgtggcg ctatctctcc ttcagacgca tccggcgact
    38221 tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca
    38281 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag ttttggttat
    38341 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctag tgccgcccac accaaagacg
    38401 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
    38461 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
    38521 agatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt ttcagtccat
    38581 taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
    38641 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
    38701 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
    38761 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
    38821 cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt
    38881 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
    38941 agcgacacgg aactgacgaa tcagaaccac ggtctttttc ttcgcgttgt acaggaggat
    39001 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt agccatcttt
    39061 gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
    39121 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat
    39181 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
    39241 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
    39301 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
    39361 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
    39421 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
    39481 gcatgacaaa ttcactgccc tcaatcggta caagtcgccg gcgcccctgg gcatccggct
    39541 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa
    39601 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
    39661 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
    39721 cgccgtggcg cagcgcggta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
    39781 cgacgttgag tccggcggta ttgataaacg gctcttccgg tagctcttcg agtcccatca
    39841 cctgtttagt gttggcgatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
    39901 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
    39961 gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
    40021 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
    40081 aaggaatgcc gaaagtgagc aagccaccaa tttctggatg gcgatcgtac accgtcaccg
    40141 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggaccc gcaccgataa
    40201 tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa cctttcgcca
    40261 acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc
    40321 gaatagtaca ggcaccttca cacaaacggt cctgcggaca aacgcgtccg gtaatttccg
    40381 gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
    40441 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
    40501 cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg atcggcacgg aatggcagat
    40561 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
    40621 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac
    40681 tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc
    40741 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg
    40801 caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc
    40861 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg
    40921 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
    40981 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
    41041 tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
    41101 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
    41161 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
    41221 accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg gcaatgttca
    41281 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
    41341 tgactaaatt gcgttttagc gaactggaac attaatgatt ttttgtggag aagacgcgtg
    41401 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
    41461 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
    41521 aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc
    41581 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcat
    41641 cggtattaac caatctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    41701 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    41761 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    41821 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    41881 ttttcacgct ggtctttttt actttgcgcc gtatacgcca tcaggtggtt gccccgctga
    41941 atcagctggt taccgccagt caacgtattg aacacgggca gttcgactcg ccgccgctgg
    42001 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag atgtcgagcg
    42061 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    42121 acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc gcaggcgctg aacacaagcc
    42181 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcggctg
    42241 aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa ccaaacccgg
    42301 aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
    42361 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg tcgatgctgg
    42421 gacgcggttt gtactttaat caggcgcaaa agcattttca gcagttattg ttgatggaag
    42481 aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt tcttacttac
    42541 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
    42601 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
    42661 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
    42721 atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg ccaaccctgg
    42781 cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gtgctgaatg
    42841 cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg ccggacggca
    42901 atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa gaacccgaag
    42961 gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctgggtggg acgctgactt
    43021 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctct gcggagggtg
    43081 aggaaagtca gttgatgtaa tgcctcctac tgaccaaaga atacttgcac ttaaggttca
    43141 gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc
    43201 ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg aggtcctctt
    43261 ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg
    43321 tgtctgacag ggaccctggc gattttttca ttgcccgttg aacaataccc cgatctcgcg
    43381 ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac
    43441 accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat gtatatgtca
    43501 tctcagagca gtggcatcgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat
    43561 ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg
    43621 caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa tattctgacc
    43681 attgctttcg tctctaccga tggttcgatg gataaacagg atattgccga ttatgttgcc
    43741 agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt
    43801 tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg
    43861 gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt
    43921 ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg
    43981 ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcaaga cggctcagag
    44041 gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc
    44101 cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg cgcaaacgaa
    44161 atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta cttcccgcac
    44221 ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac
    44281 gtggtgaaaa cgctgctgga agctatcgcc ctggttttcc tcgttatgta tctgttcctg
    44341 caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc
    44401 ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggta
    44461 ctggcgatcg gtctgctggt ggatgacgca atcgtggtgg tggaaaacgt cgaacgtatt
    44521 atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag
    44581 ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtaccaat ggccttcttc
    44641 ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg
    44701 ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacact acttaagcca
    44761 ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa ccagatgttt
    44821 aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg cagcctgcgc
    44881 tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg
    44941 acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca attgcccagc
    45001 ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcacc
    45061 catgaaaaag acaacatcat gtcggtgttt gccaccgttg gttctggccc tgggggtaac
    45121 gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag
    45181 accggcacct cgtttgccat tatcgagcgt gcaacaaagg cgtttaacaa gattaaagaa
    45241 gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc tgcgggtttt
    45301 gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc agcacgtaac
    45361 cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc
    45421 gacgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt
    45481 gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac
    45541 tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg
    45601 ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc
    45661 tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc gtctggaacg ctataacggt
    45721 tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg
    45781 gatattatgg aatcgttagt gaagcagctg ccaaacggct ttggtctgga gtggacggcg
    45841 atgtcgtatc aggagcgact ttccggcgcg caggctccgg cgctgtacgc catttccttg
    45901 ctggtggtat tcctgtgtct ggctgcgttg tatgaaagct ggtcggtgcc gttctcggta
    45961 atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg caacctggat gcgcgggctg
    46021 gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac
    46081 gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa
    46141 gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt
    46201 atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg
    46261 gtgggtactg gcgtaatggg cgggatgatt tcagccacta ttctggctat ttacttcgtg
    46321 ccgctgttct ttgtgctggt gcgccgccgc ttcccgctaa agccgcgccc ggaataagca
    46381 ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc
    46441 agcgggttct tgctgtttat actttgtctc aggaattact tacgaagcat aacttcgata
    46501 aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt
    46561 attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc
    46621 tccgggggga caaatcttat gtaaatacta tggtcctgcg gtgatgattt gtatgtgata
    46681 cacagcaaca ttt
//
LOCUS       DAZPJP010000009        46014 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPJP010000009 DAZPJP010000000
VERSION     DAZPJP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.009"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..715)
                     /locus_tag="ACHU9J_000461"
     CDS             complement(<1..715)
                     /locus_tag="ACHU9J_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1904106.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="ACHU9J_000462"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="ACHU9J_000462"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1904107.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="ACHU9J_000463"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="ACHU9J_000463"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1904108.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="ACHU9J_000464"
     CDS             complement(3649..4704)
                     /locus_tag="ACHU9J_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1904109.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="ACHU9J_000465"
     CDS             complement(4714..5502)
                     /locus_tag="ACHU9J_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1904110.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="ACHU9J_000466"
     CDS             complement(5520..6572)
                     /locus_tag="ACHU9J_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1904111.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7445)
                     /locus_tag="ACHU9J_000467"
     CDS             complement(6603..7445)
                     /locus_tag="ACHU9J_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1904112.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7432..8313)
                     /locus_tag="ACHU9J_000468"
     CDS             complement(7432..8313)
                     /locus_tag="ACHU9J_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1904113.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8334..9644)
                     /locus_tag="ACHU9J_000469"
     CDS             complement(8334..9644)
                     /locus_tag="ACHU9J_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1904114.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9658..11189)
                     /locus_tag="ACHU9J_000470"
                     /pseudo
     CDS             complement(9658..11189)
                     /locus_tag="ACHU9J_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="ACHU9J_000471"
     CDS             complement(11401..11715)
                     /gene="pspE"
                     /locus_tag="ACHU9J_000471"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1904115.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="ACHU9J_000472"
     CDS             complement(11790..12011)
                     /gene="pspD"
                     /locus_tag="ACHU9J_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1904116.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="ACHU9J_000473"
     CDS             complement(12020..12379)
                     /gene="pspC"
                     /locus_tag="ACHU9J_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1904117.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="ACHU9J_000474"
     CDS             complement(12379..12603)
                     /gene="pspB"
                     /locus_tag="ACHU9J_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1904118.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="ACHU9J_000475"
     CDS             complement(12657..13325)
                     /gene="pspA"
                     /locus_tag="ACHU9J_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1904119.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13492..14469
                     /gene="pspF"
                     /locus_tag="ACHU9J_000476"
     CDS             13492..14469
                     /gene="pspF"
                     /locus_tag="ACHU9J_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1904120.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="ACHU9J_000477"
                     /pseudo
     CDS             complement(14587..15852)
                     /gene="puuE"
                     /locus_tag="ACHU9J_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="ACHU9J_000478"
     CDS             complement(15890..17170)
                     /gene="puuB"
                     /locus_tag="ACHU9J_000478"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1904121.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="ACHU9J_000479"
     CDS             complement(17172..18662)
                     /gene="puuC"
                     /locus_tag="ACHU9J_000479"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1904122.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="ACHU9J_000480"
     CDS             complement(18812..19369)
                     /gene="puuR"
                     /locus_tag="ACHU9J_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1904123.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="ACHU9J_000481"
     CDS             complement(19396..20160)
                     /gene="puuD"
                     /locus_tag="ACHU9J_000481"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1904124.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20372..21733
                     /gene="puuA"
                     /locus_tag="ACHU9J_000482"
     CDS             20372..21733
                     /gene="puuA"
                     /locus_tag="ACHU9J_000482"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1904125.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21882..22046
                     /locus_tag="ACHU9J_000483"
     CDS             21882..22046
                     /locus_tag="ACHU9J_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904126.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22036..23421
                     /gene="puuP"
                     /locus_tag="ACHU9J_000484"
     CDS             22036..23421
                     /gene="puuP"
                     /locus_tag="ACHU9J_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1904127.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23555..23800
                     /locus_tag="ACHU9J_000485"
     CDS             23555..23800
                     /locus_tag="ACHU9J_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1904128.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24113..25756
                     /gene="sapA"
                     /locus_tag="ACHU9J_000486"
     CDS             24113..25756
                     /gene="sapA"
                     /locus_tag="ACHU9J_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1904129.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGRATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25753..26718
                     /gene="sapB"
                     /locus_tag="ACHU9J_000487"
     CDS             25753..26718
                     /gene="sapB"
                     /locus_tag="ACHU9J_000487"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1904130.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26705..27595
                     /gene="sapC"
                     /locus_tag="ACHU9J_000488"
     CDS             26705..27595
                     /gene="sapC"
                     /locus_tag="ACHU9J_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1904131.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27595..28587
                     /gene="sapD"
                     /locus_tag="ACHU9J_000489"
     CDS             27595..28587
                     /gene="sapD"
                     /locus_tag="ACHU9J_000489"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1904132.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28589..29395
                     /gene="sapF"
                     /locus_tag="ACHU9J_000490"
     CDS             28589..29395
                     /gene="sapF"
                     /locus_tag="ACHU9J_000490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1904133.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29463..29816
                     /locus_tag="ACHU9J_000491"
     CDS             29463..29816
                     /locus_tag="ACHU9J_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1904134.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30184..30972
                     /gene="fabI"
                     /locus_tag="ACHU9J_000492"
     CDS             30184..30972
                     /gene="fabI"
                     /locus_tag="ACHU9J_000492"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1904135.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31117..32243
                     /locus_tag="ACHU9J_000493"
                     /pseudo
     CDS             31117..32243
                     /locus_tag="ACHU9J_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32311..34245
                     /gene="rnb"
                     /locus_tag="ACHU9J_000494"
     CDS             32311..34245
                     /gene="rnb"
                     /locus_tag="ACHU9J_000494"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1904136.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34323..34426)
                     /locus_tag="ACHU9J_000495"
                     /pseudo
     CDS             complement(34323..34426)
                     /locus_tag="ACHU9J_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34480..36470
                     /gene="pdeR"
                     /locus_tag="ACHU9J_000496"
                     /pseudo
     CDS             34480..36470
                     /gene="pdeR"
                     /locus_tag="ACHU9J_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36452..36580)
                     /gene="ymiD"
                     /locus_tag="ACHU9J_000497"
     CDS             complement(36452..36580)
                     /gene="ymiD"
                     /locus_tag="ACHU9J_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1904137.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36617..36790
                     /gene="yciZ"
                     /locus_tag="ACHU9J_000498"
     CDS             36617..36790
                     /gene="yciZ"
                     /locus_tag="ACHU9J_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1904138.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36880..37629
                     /locus_tag="ACHU9J_000499"
     CDS             36880..37629
                     /locus_tag="ACHU9J_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1904139.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37898..38116
                     /gene="osmB"
                     /locus_tag="ACHU9J_000500"
     CDS             37898..38116
                     /gene="osmB"
                     /locus_tag="ACHU9J_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1904140.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="ACHU9J_000501"
     CDS             complement(38242..38568)
                     /gene="yciH"
                     /locus_tag="ACHU9J_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1904141.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="ACHU9J_000502"
     CDS             complement(38568..39305)
                     /gene="pyrF"
                     /locus_tag="ACHU9J_000502"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1904142.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="ACHU9J_000503"
     CDS             complement(39498..40667)
                     /gene="lapB"
                     /locus_tag="ACHU9J_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1904143.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="ACHU9J_000504"
     CDS             complement(40674..40982)
                     /gene="lapA"
                     /locus_tag="ACHU9J_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1904144.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="ACHU9J_000505"
     CDS             complement(41131..41895)
                     /gene="pgpB"
                     /locus_tag="ACHU9J_000505"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1904145.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42065..42655
                     /gene="ribA"
                     /locus_tag="ACHU9J_000506"
     CDS             42065..42655
                     /gene="ribA"
                     /locus_tag="ACHU9J_000506"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1904146.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="ACHU9J_000507"
     CDS             complement(42719..45394)
                     /gene="acnA"
                     /locus_tag="ACHU9J_000507"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1904147.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45558..45653)
                     /gene="ymiC"
                     /locus_tag="ACHU9J_000508"
     CDS             complement(45558..45653)
                     /gene="ymiC"
                     /locus_tag="ACHU9J_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1904148.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45767..45934)
                     /gene="yciX"
                     /locus_tag="ACHU9J_000509"
     CDS             complement(45767..45934)
                     /gene="yciX"
                     /locus_tag="ACHU9J_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1904149.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45937..>46014)
                     /locus_tag="ACHU9J_000510"
     CDS             complement(45937..>46014)
                     /locus_tag="ACHU9J_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1904150.1"
                     /translation="AWLAVFLGSALFWVVVALLIWKVWG"
BASE COUNT        10877 a        12177 c        11596 g        11364 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca gacagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat taccccttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc gcttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca acccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtgtcgt aaatgcccag cgcggtgatg attttgaaca gcggcaccac cagcaaaatg
     7081 ccagagaaca tatacaccgt gtaaaagctg gcgttgatgg tcatccgacc tttaaagcgc
     7141 aggcgggaaa gcgcgtaagc cccaagaatg ccgaggaata ccgccaccac ggatgaaacc
     7201 acagacacca ccagactgtt acggaagtag tcgacaaacg ggaaaatcat cgggttaaaa
     7261 atgtcgacgt aatgctccag cgtccactgc tgcggcagca gcgtaggatg cagtgagatc
     7321 gcctctttcg cgcccttgaa cgaggtcatc agcatcacaa aaaatggaaa cagcgtgatg
     7381 atgagaaaca gcgccagccc gcagtaaaaa ccgatgcgac tgagtgtgcg tttatttgtt
     7441 gccattgagg ttcacccgtt tcctggtcag cagaatgaca gcgaaaataa tgatgaagag
     7501 caccaccgaa atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg ttttgtagag
     7561 atagacgccg agaatatcga ccttggtggt cagcagataa acatcagcaa acatgtagaa
     7621 catccagata gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg cgggcagcgt
     7681 gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgccgctt cgtacagcga
     7741 tttgtcgatg gtctgcaaaa tcgccagaaa cgagataaag gcatacggga agtagcgcca
     7801 gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc acagcggtgc
     7861 ctgctcatag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt agccgttgtt
     7921 gaacatgtat ttccaggcaa acaccagcga aatggacggc gttacgtagg agaggatcac
     7981 cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg aaaaacatcg ccactgccag
     8041 cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca tccacagcga
     8101 gtgccagaag ccgggatcgg agaggatacg cacatagtta ctcaccccaa caaaggttga
     8161 ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg acaccattgg
     8221 ccaggccacc agaccaccca gcagtaataa gctgggcgcg agaagcagca gcgcaaaggg
     8281 catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg ggtttagtgc
     8341 tgttcgatca gttcatccag ctttttctgg ctcgcttgca gcgtgccagg gagatcggct
     8401 ttacccacgg tgacgttatg caccatggag ctcaccacgc cagaacccgt cacatcaccc
     8461 atgcgggtaa agtttttatc ccctactgcg ccaaagacct gaatatttgg cagttcaccg
     8521 attagctgat tcggtagttc acccagcgcc ttaataacgt cgttgtcttt ccaggtggcg
     8581 gtagtgacaa ccgctttatt caccggcagc gcagcacctg gcgacatcat cacccagtcg
     8641 gcaatgttgt ctgcctgctc cataaaggtg acaaattttt ctgctgcttc cgtctcttcg
     8701 gccttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
     8761 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaattac agccggaagg
     8821 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
     8881 atgatgtcgt tagaacccgg catgtcgtta gaacccggca tagtgttggc agcaaggtcg
     8941 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
     9001 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
     9061 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
     9121 gcaacgtcca gtagctgttg ccagtttttc ggctcctcca gccccgcttt tgccagcaca
     9181 tctttgcgat accagatgcc gccaatccag gcgctgacgg gaacaccggt ccaggcactc
     9241 ccatcttcgg tgcgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg
     9301 ctgatgaccg tggcaaccgt cttgcgatcg ataagctgct ctttatccat aactttggcg
     9361 tagtcatggc tggtttcgat cacttccggc agcgagccgc tacgtgaaag ggtgatgact
     9421 ttggtgttat aagcgtcctc ttccacgggc acttgtttaa cggtgatacc tgggttttct
     9481 ttttcaaaac gggcaatcaa tttgctgata actgcctgac gctcctgctc caccgaagaa
     9541 tgcataaatt cgatggatac atgggtttta ttttcttctt tacagcctga aatcagggcg
     9601 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
     9661 tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccaata gatcaacttc
     9721 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
     9781 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
     9841 tgaattaatc gtatcgatgg taaaattatt atccggatga aactcgttat ggctgcgacg
     9901 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
     9961 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    10021 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    10081 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    10141 ttcatcgcta ctctcacggc ggcttaacgc atccatatgt gagaggcgag gaagttaaac
    10201 caggtggtgt tgctggaagg tagtgtcagg ttttgcgccc acgcacaaag cgcctcaacg
    10261 ttctgttttt gcaccgcatg cagcaccagt ggcggcagcg agaactggta caccatatgt
    10321 gcttcgtcat cgcctgcgcc aaagtaagca atgttgtctt tatgcggaac attggtctcg
    10381 gtaatgatca ctgtacctgg cgcaacgtta tcaataatcg accgtaacag tttgataatc
    10441 agatgtgttt tttccagatg gatgcagctt gttcccggct ctttccacat aaagccaacg
    10501 gcatccaagc ggacatactc agcacctttc gcaaggtaac agagtaaaac atccaccatc
    10561 gccagcaaca cttcagggct acggtaattc aggtcaattt gatcgtcact aaaggtggtc
    10621 cataaatggc gcgttgaatg atcgcgcatc tggaatggcg ttaataacgg taacgcacgc
    10681 gggcgagtga cggcgctgag atcggtttgc ggatcaaccg caataaaaaa atcttcaaaa
    10741 cctggctgct gttgtaaata gtttttaaac cattcacttt ttgccgacat atggttgcag
    10801 acaaaatcaa acattaaatg actgcattca ccgagttgct gaatatcctg ccactccccc
    10861 gcttcactgg cgacctgatg ataatcaatt accgaaaacc catcatcaga tgaacatgga
    10921 taaaaaggta ataaatgaac atgtgaaaaa atgctttgca gccattgatg gtaaaactga
    10981 ttaaatgtgg gtaatggttt taaatcattg ctgtgaaatt gatcggcata ggtaattaac
    11041 acgacatcac tttcatccca gtgtttttta cgtcgctgtg taattaatcg tttcgcactc
    11101 tcaagacgcg ttacaagttt ctgtaaatga gttgcggtaa atgttccacc gtagatttcg
    11161 tccaggtaat ccgtaatttt ctgtttcatg ttgccttttt gtggtagcgg tcccatgaaa
    11221 cgagagacta ctccgcagaa aataaactgt caattgagca caagggtttt agtcacgcag
    11281 gaaatcattt tttaccaggg aaaaagcgta tttgtgagct gacgcaaata atctatgggg
    11341 atgtgaataa ggtgtgacag aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt
    11401 ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt
    11461 atatcccatc tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata
    11521 cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt
    11581 caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacgaac
    11641 atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa
    11701 gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct
    11761 gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta
    11821 gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga
    11881 gcaactgatt ttatcgccca gcccgctaca cccgccgggc catagcgcag tgcggtaaga
    11941 agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag
    12001 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
    12061 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
    12121 cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
    12181 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
    12241 agaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg
    12301 caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat
    12361 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga
    12421 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg
    12481 ctgctgctca ctttgcgaca attcatcgcg accagaacga ttgctgtaat gcagccataa
    12541 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct
    12601 cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat
    12661 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg
    12721 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
    12781 gcttccgctt ccatctggtc aattcgacgt tcgaaagatt cgaaacgagc cattgcttca
    12841 tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga
    12901 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgcca
    12961 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagggac
    13021 ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc
    13081 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
    13141 tcaatacggc gagtcagctg tttcttttct gccaacgcac gcgccgaagt agaacgtact
    13201 tcaaccagtg tatcttccat ctcctggatc atcagacgca ccagtttctg tggatcttcc
    13261 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
    13321 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca
    13381 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
    13441 aacatgccag gatgaattag cgaattacac taacaagtgg cgaatttcat catggccgaa
    13501 tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
    13561 catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag
    13621 ctgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt
    13681 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg
    13741 gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg
    13801 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg
    13861 attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg
    13921 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggcac ttttcgcgct
    13981 gacctgctcg accgactggc ttttgatgtt gtacaactgc caccactgcg cgagcgcgaa
    14041 agcgacataa tgttgatggc agaacacttt gccatccaga tgtgtcggga aatcaagctg
    14101 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg
    14161 ggaaatattc gtgaattgaa aaacgcggtg gaacgttcag tgtatcgcca cggcaccagc
    14221 gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc tgaagacgct
    14281 atcgccgttt cagaaaccac ctcgcttccg acgctgccgc tggatttacg tgagtttcaa
    14341 atgcagcagg aaaaagagtt gctgcaactc agtttgcaaa aggggaaata taaccagaag
    14401 cgcgcagctg aattgctggg gttaacctac catcagttcc gcgcgttgtt gaaaaaacac
    14461 cagatttagc gaagttgggc aatgcgattc gccggatgcg gcgtgaacgc tttatccggc
    14521 atacataatc aacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt
    14581 gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc
    14641 cgggatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag
    14701 gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc
    14761 gcccgtttgc ggatcgttaa actctgccgc aatcatcgcc cccaggccgc gtaccgcagc
    14821 aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg
    14881 attcgcgcgt tcgcagagtg attctttgtt gataatattg agcaccgcgt gcgcggcagc
    14941 caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat
    15001 aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat
    15061 cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc
    15121 aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg
    15181 aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc
    15241 gaaaataatg gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc
    15301 gtcgagggag tcctatgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac
    15361 cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat
    15421 ggtcatatat gtacgaccgt ggaagccacc gctaaacgca atcacgccag ggcgtccggt
    15481 atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcaccggtag tgaagaacgc
    15541 ggttttggcc tgcccgctca ccggggcaag ggcgttgatt ttctccgcca gggtgacgta
    15601 gctttcatac ggcacaatct gatacgcggt gtgggtaaac tgttgcagtt gctgctccac
    15661 cgccgcgacc agatcagggt ggcgatgtcc ggtattcagc accgcaatgc ctgcggcgaa
    15721 atcgatgtac tcgttgccct caacatcctt cagagtagcg ttttcagccg actgggcgaa
    15781 gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc
    15841 attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca
    15901 gacgatcgcg caggctgtac caggctgcgc ccatcgcggt aaacggtact cgcagcgtgc
    15961 gcccgccggg gaacgggtaa tacggcagat tggcgaaggc atcgaaacgt tcggcgtcgc
    16021 cgcgcagcag ttcggcaatc aaacgtccgg cgagatgagt acaggtgacg ccgtggccgc
    16081 tgtaaccctg catgtaatag atgttggtat caaggcgacc aaactgcggc atgcgcgaca
    16141 gggtcagcag gaagttgccc gtccagcggt agtcgatttt gacgcccttc agttgcggga
    16201 aggttttcag cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt
    16261 agaccacgcc gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca
    16321 gataattaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg
    16381 acagccgttc agtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg
    16441 gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct
    16501 gacctttggc agtgcgcacc accgctggcg tggtgtgctg gatctgcgtc acggcagaaa
    16561 gctcatacac gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta
    16621 gcgggtgaat atgcccaccg ctgtgatcca acagtgcgcc agtgtagcga tcgctggcga
    16681 cctcgcggcg aatggcgtta gcgtccagca gctccagctg tttattgccg tagcgttccc
    16741 agttctcttt ctgctcttcg agtgtggcga gctgtttatc attcatcgcc acaaacaggc
    16801 cgccggggcg gtagtcgcag tcaatctgat agcgtttgat acgttcgcgg atgatctcgc
    16861 caccctcgaa catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga
    16921 tcacgtcgat gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc
    16981 caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca
    17041 gcgcggagga gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact
    17101 cattcagtgt gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat
    17161 gttcggtcat ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca
    17221 agggcatgca gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg
    17281 gtcatatcgc cgtcgttgta gttattgacg aagacggaac cggctttcag gcggcggctc
    17341 atacggtgcg cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg
    17401 tcgttggcaa gctgtagcgc ctggtcttct gatgtgaaac gcgtgaccac cagcaccgga
    17461 ccgaaaatct cttcgcggct taaggacgca ttcgggtcaa catccacaaa gatggtcggg
    17521 ccgatggcgg cagccagctc agcgttacga ccatccagca gcagttgccc tttgctttcg
    17581 ccttcacgaa taaagctatg gaccgagtcg gcgtgggcgc aatcgattaa ggtgcccatg
    17641 gtggttacgg tatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg
    17701 gctaagaatt catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc
    17761 tggccctggt tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga
    17821 caatcggcga aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac gcgtttcatg
    17881 ttgctatcac ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg
    17941 atggcatcga tatcgttatg acgcgaaagc gcctgcccgg cttcatgacc ataaccgctc
    18001 actacgttca gcacgccatc cggcagcccc gcttcttctt ttgccagccc ggcgagacga
    18061 atcgcactga gtggtgattt ttcagacggt tttagaatca cgctgtttcc cgccgccagc
    18121 gccgggccga gtttccagca agtcagcagc agcgggaagt tccacggcac gatggcggca
    18181 atcacgccga ccggttcacg cacgatcatc gccagctcat ggctactggt ggtcgccact
    18241 tcgccataca ctttgtcgat cgcttcggcg taccagcgaa tggcgcgcgc cgcgccggga
    18301 atatcatcac gcagactgtg acgaatcggt ttgccggtgt cgagagtttc cagcagtgcc
    18361 agctcttcgg cgtgggcttc cattaaatcg gcgagtttat tcagtaccgc tttacgttta
    18421 gccggagaag agagtgacca gtcgccgcgt tcaaatacgc cgcgtgctgc gctcaccgca
    18481 tggtcgatat cgacgctctt gccgcgggca attttcgcca gcggtgcctg ggtgaccgga
    18541 tcaacggttt caaaggtttc attttccgcc gcagcagtat attcaccgtt aataaataag
    18601 cggttttcaa tggcgagact taacgcttta tcctgccagt aagccagatg atgaaaattc
    18661 attatgactc ctgtttcacg tctatcagat atatgcagtt tgtgaacgtc agggcgcaga
    18721 gatccccggg ccgcatgccc gtttatttct ggcgacattc tttatggatt gccgggcgca
    18781 ttgcgcttac ccggcttaca gaacaaaaag attaaaacgt ggtgggcgta tgggcgctga
    18841 taattcggca aatacctgcc gacgtattac tgaaactgtg cgggatgccg gtattaatgg
    18901 cataactttg ccccgcgacg aggtggtaat cctgaccatt aatcgtcaga acaatttcac
    18961 cttccagtac tgtgcctatt tcctcaccct gatgcttaat tctttcccca gtggttgtgc
    19021 ccggctggta cgtttcaaag atcatcgcca gcgtgcgatt cgggttaccg ttatgaacca
    19081 gcttcattga cacaccctga ctgcccatct caattaagtc gtcctgatta atgacgacct
    19141 gcggctcatc aggtttttcc ggctcggaaa agaattccga gagtgacaga ccatacacct
    19201 tcagcagctt ttgcagcgta ctgatggcag ggctgacttt atcttgttct atcgtactga
    19261 tagcactgtg agtcagcccg gagagttcgg cggcacgacg ttgtgaaagc ccctgctgct
    19321 ggcggatttc cgacaagcgt tttcctggcg ccagtccctc atcactcata tttgcatttc
    19381 cttaaactgt agtggtcaga gtcgctgttt ttcagcgata tggtgctgac aagcggtgat
    19441 aaagccctcg aacaatatac gcgaaagcgc gtactcgcta ctgttccatt ccgggtgcca
    19501 ctgtacgccc agcgcaaaag gatgattgat gacgctaacc gcctcaacta agccatccgg
    19561 cgagcgagct tcaacacgca accgtgggct aacgaccttc gccccttgtc catgtagaga
    19621 gtttacccaa aagttgctac attcaggtaa caacgcagac agtaatcccc cctcttcaac
    19681 ctgaacttca tgcgacggtg catattgctg ttccaccggc agttcgggat cttcccgatg
    19741 ttccagcaat tcaggctgtt cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac
    19801 cagttcttgt aaaccccggc agatggcgaa aatggggatg cgcctttcga gtgcggcatt
    19861 aattatcgcc atgctcagaa gatcacgccc gggatcggcg tcaggctcat cgccgttttc
    19921 accatatagg tgcggctgca cattgctggg actaccagga agataaatgc catcgagttt
    19981 cggcaaaagt tgttcaagta acgacggttc cgccagcgca tgtggtagcg caattggcaa
    20041 gccgcctgcg tggatgatgg cattcaggta cttttcttgc agagtctggg tcgcatgacc
    20101 cttaagcctg ttcctgcaca ttacgacacc gataaccgga ttgttcatta tattttccat
    20161 gctcaatctc acaaagtgga ctaaattatc gccattactg cgctttaaat gacctttatg
    20221 ttcaatattt tttcaatcta gcagtgggtt ttcatttttg caaactcaat ttaacatttg
    20281 acaaacattt agtttgcata cagattcgaa tggtggtcat tatattttac gctttgataa
    20341 cgagcggaaa acaaaccaaa ggcgaagaat catggaaacc aatatcgttg aagtagagaa
    20401 ctttgttcag cagtcagaag agaggcgggg tagcgccttt acgcaggaag tgaaacgcta
    20461 cctggagcgc tacccgaata cgcaatatgt tgatgtttta cttactgatt tgaatggttg
    20521 cttccgtgga aaacgtatcc ctgtttcaag cctgaagaag ctcgagaaag ggtgttattt
    20581 cccggcctcg gtatttgcaa tggatattct gggcaacgta gtagaagaag caggtctggg
    20641 tcaggaaatg ggcgagccgg atcgtacctg tgttcctgtt ctcggttcct taactccttc
    20701 tgccgccgat ccagagttta tcggtcagat gctcctgacc atggtcgatg aagatggcgc
    20761 tccctttgac gttgagccgc ggaacgttct taaccgcctg tggcagcagc tgcgccagcg
    20821 cgacgctgaa gggaatctgc aaccgccctg cgcgcctggc accgatgacc gcaatacaca
    20881 aagccaggtt tactccgttg ataacctcaa ccacttcgcc gacgtgctca atgatattga
    20941 tgaactggcg cagttgcagc tgattccggc agatggcgcg gtcgctgagg cctcgccggg
    21001 tcagtttgaa atcaaccttt accatactga taacgtgctg gaagcctgcg atgatgcgct
    21061 ggcactaaaa cgcctggtgc gtctgatggc aggaaagcat aagatgcacg ccacttttat
    21121 ggcgaagccg tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa
    21181 taatcgtggc gagaatgtgc tttctgacac agaaggcgaa gattcgccgc tgctgaaaaa
    21241 gatgctcgcc gggatgattg acctgatgcc gtcgtcgatg gcgttgctgg caccaaacgt
    21301 gaactcgtat cgccgcttcc agccgggaat gtatgtgccg acgcaggcgt cgtggggcca
    21361 taacaaccgc accgtcgccc tgcgtattcc gtgcggcgac cgtcataatc accgcgtgga
    21421 atatcgcgtg gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg
    21481 tattttgcat ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gcaacgggct
    21541 ggaacaggaa ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga
    21601 gaatgatcac ctgcgccgct atttaggcaa acggttttgc catgtgtatc acgcctgtaa
    21661 aaatgatgag ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt
    21721 gaaaaacgcg taattcccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga
    21781 catttgctgg taatccagac atttcccgga tgtcgcgtta tggagcgcag gcggcaacgg
    21841 gcttccgtta acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc
    21901 cgcatggcga gggaggctgc gtatgcgccc ctcctgctac tgactggact ccgccccctt
    21961 tgcttccctt gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca
    22021 ggggaggcag caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa
    22081 acccgtctgc gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc
    22141 acgccgatga ccgtatttga tacttttggt attgtctccg gcattagcga cggtcacgtt
    22201 ccggcgtcct atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa
    22261 ctggttcgcc agtttccgga ggccggttcg gcctatacct acgcgcaaaa gtcgattaac
    22321 ccgcacgtcg gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg
    22381 atcaacgttt tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg
    22441 gtgtgggtgg taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg
    22501 gtcgctaact tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc
    22561 ttcctggtgg ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag
    22621 ccgtttatca gcgagaacgc gcacctgatc ccgattatta ccggtgcgac gattgtctgt
    22681 ttctcgttcc tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca
    22741 cgcgtgatcc cgaaagccat cttcctgacg gcggtatacg gcggcgttat ctttatcgcg
    22801 gcgtcgttct ttatgcagct gttctttccc gatatcagcc gctttaaaga cccggacgcc
    22861 gcactgcctg aaattgcgct gtacgtcggc ggcaagctct ttcagtcgat tttcctctgc
    22921 accacgtttg tgaacacatt agcgtctggc ctggcttcac atgccagcgt gtcgcgtctg
    22981 ctgtatgtga tggggcgtga caatgtgttt ccggagcgcg tgtttggcta tgtgcaccca
    23041 aaatggcgga ctccggcact caacgtcatt atggtcggga ttgtcgcgct gtcggcgctg
    23101 ttctttgatt tagtcaccgc gacagcactg attaacttcg gtgctctggt ggcgtttacc
    23161 ttcgtgaatc tgtcggtgtt taatcatttc tggcggcgta aaggaatgaa taaaagctgg
    23221 aaggatcact tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg
    23281 tgggttaacc tagagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc
    23341 gcatatttgt ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga
    23401 acgccggtga gcgaaacgta atagccggat gcgcggcgca ttatcctgcc tgagggtaac
    23461 gcgccgcgcc attaactata aaaagatgta tgacccggat cacgagtgct atcatccgcc
    23521 cttatgctta gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata
    23581 ttttgatatt gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg
    23641 cacaccgctg gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat
    23701 cagcgaagaa gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga
    23761 tgagatcgca gagtttttga atcaatgggg taatgagtaa ttatttaatt gcagacgatc
    23821 ggtcagtatc gttgtccgga taaagcgttc acgccgcatc cggctaacaa tgacacgcac
    23881 tgcagcgtgc tttaatgggt ttctcacgat cgtaccgctc tgctcctcgc taagttgttc
    23941 tgtgagttat agttatagag aaatcacggg tacgaatgtt gaacagatgg cgacgccacc
    24001 tgttcaacga ccaaccacac ggccctcatt acgacccctg aacacaaacg gtaatggcaa
    24061 atgtcattaa atattagtaa gatagcctct ttacataaaa tcccttaata ttatgcgcca
    24121 ggtattatcg tctcttttgg tgattgctgg acttgtgagt ggtcaggcaa tcgccgcgcc
    24181 tgaatccccc ccgcatgctg atatccgcga cagcggtttt gtctattgcg tcagcgggca
    24241 agtcaacacc tttaacccat ccaaagcgag cagtgggtta attgtcgata cccttgccgc
    24301 ccagttttat gatcgactgc tggatgtcga tccctatacc tatcgcctga tgccggaact
    24361 tgccgaaagc tgggaagtac tcgacaacgg cgcgacctat cgcttccacc tgcgtcgcga
    24421 tgttccgttt caaaaaaccg actggtttac tcccactcgt aaaatgaatg ccgacgatgt
    24481 ggtgtttacc ttccagcgaa tttttgaccg caacaacccg tggcataacg tcaacggcag
    24541 caacttcccc tacttcgaca gcctgcaatt tgccgataac gtgaaaagcg tccgcaaact
    24601 ggataatcat accgttgagt tccgactcgc ccagccggat gcttcttttt tgtggcacct
    24661 cgcaacccat tatgcttcgg tcatgtcggc agaatatgcc cggaagttag agaaagaaga
    24721 tcgccaggag caactcgacc gtcaaccggt cggcaccggt ccgtatcagt tgtcggaata
    24781 ccgcgccggg caatttattc gcctacaacg tcatgatgac ttctggcgcg gtaaaccgtt
    24841 aatgccgcag gtagtggtgg atttaggctc cggcggcacc ggacgtctgt cgaaactcct
    24901 gaccggggaa tgcgacgttc tggcctggcc tgctgccagc cagctatcca ttttgcgtga
    24961 cgacccgcgc ttgcgtttaa cgctgcgtcc tgggatgaac gtcgcctatc tggcatttaa
    25021 caccgccaaa ccgccgctaa ataatcccgc tgtccgccat gcactggcac tggcgattaa
    25081 taaccagcgc ctgatgcaat ccatctatta tggtacggct gaaacggcgg cctctatttt
    25141 accgcgcgcc tcgtgggcct atgacaacga ggctaaaatt actgaataca atccggcgaa
    25201 atcgcgcgaa cagttgaagg cgttggggct ggaaaactta acgctgaaac tgtgggtgcc
    25261 cacacgttcg caggcgtgga accccagtcc actgaaaact gccgaactga ttcaggcgga
    25321 tatggcgcag gttggcgtaa aagtggtgat tgtgccggta gaaggtcgct ttcaggaggc
    25381 gcggttgatg gatatgagcc atgatctgac gttatccggt cgggcgacgg acagtaacga
    25441 cccggacagt ttcttccgtc ctttactgag ctgcgcggca attcattcac agaccaacct
    25501 cgcccactgg tgcgatccga aattcgacag cgtgttgcgt aaggcgctct cctcgcagca
    25561 gctggcggcg cgtattgaag cctatgacga agcgcagagt attctggcgc aggaattacc
    25621 tattctgccg ctggcgtcgt cattgcgttt gcaggcctat cggtacgata tcaaaggtct
    25681 ggtacttagc ccgtttggta acgcctcctt tgctggggtg tatcgcgaga aacaggatga
    25741 ggtgaaaaaa ccatgattat cttcacctta cgccgcattt tgttattgat tgtcaccttg
    25801 ttcctgctga ccttcgttgg cttcagcttg agctatttca cccctcatgc gccgttacaa
    25861 ggcgcgtcac tgtggaatgc ctgggtgttc tggtttaacg gcctgatcca ctgggatttt
    25921 ggtgtttcca gcattaacgg tcaaccgatt gccgaacagt taaaagaggt cttcccggcg
    25981 acgatggaac tgtgcatcct cgccttcggt tttgcgctga ttgttggcat ccccgtgggg
    26041 atgattgccg ggattacgcg ccataagtgg caggataact tgatcaacgc catcgcctta
    26101 ctgggctttt caatcccggt gttctggctg gcgcttctgt tgacgctgtt ttgttcactc
    26161 acgctaggtt ggttgcccgt ttcagggcgt ttcgatctgc tctacgaagt gaaaccgatt
    26221 accggttttg cgttgattga tgcctggctt tcggactcac catggcggga tgaaatgatc
    26281 atgagcgcaa tccgccatat gatattgccc gtgatcaccc tgtcggttgc gccaacaact
    26341 gaagtgatcc gcctgatgcg tatcagcact atcgaagtgt atgaccagaa ctatgtaaaa
    26401 gcggcggcga cccgcggttt gtcgcgcttt actattttgc gtcgccacgt tctgcataac
    26461 gcgttaccgc cggttattcc tcgcctgggc ttacagtttt ccaccatgct gacgctggcg
    26521 atgatcaccg aaatggtttt tagctggccg ggtttagggc gctggttaat taacgccatc
    26581 cgccagcagg attacgcagc catttccgcc ggagtgatgg tgtgtggctc actggtcatt
    26641 attgttaacg tgatttctga tattttgggt gccatggcta accctctgaa acataaggaa
    26701 tggtatgcct tacgatagcg tatacagcga aaagcgcccg ccgggcacgc tgcgtaccgc
    26761 ctggcgcaaa ttttatagtg acgcctctgc gatggtcggc ctgtacggct gcgcggggct
    26821 ggctgtactg tgtatttttg gcggctggtt tgcgccttac ggcatcgacc agcaatttct
    26881 cggttatcaa ttattgccgc catcatggtc gcgctatggc gaagtttctt tcttcctggg
    26941 gactgacgac ctcggacgcg atgttttaag ccgtttactg agcggagctg cgccaaccgt
    27001 gggtggcgca tttgtggtga cgcttgccgc gacgatctgt ggcctggtac tgggcacctt
    27061 cgccggggcg acacacggcc tccgctcagc ggtgcttaac catattctgg ataccttact
    27121 ggcgatccct tcgctgctgt tggcaattat cgttgtagcg tttgccggac cgagtttgtc
    27181 ccacgctatg tttgctgtct ggctggcatt gctgccgcgt atggtacgtt cgatttacag
    27241 catggtgcat gacgaactgg aaaaagagta cgttatcgcc gcccgtctgg atggcgcatc
    27301 aacgctgaat attctctggt ttgctgtgat gccaaacatc accgccgggc tggtcaccga
    27361 gatcacccgc gcactgtcga tggcaattct cgatatcgcc gcgctgggct ttctcgatct
    27421 cggcgcacaa ctcccctcgc ctgaatgggg agcgatgctc ggtgatgcgc tggaactgat
    27481 ttatgtcgcc ccgtggactg tcatgctgcc aggtgcggca attatgatta gcgtgttgtt
    27541 ggttaacctc cttggtgatg gcgttcgtcg tgcgattatt gcgggggtgg aataatgcca
    27601 ttactcgata ttcgtaacct gaccattgaa tttaaaaccg gtgatgagtg ggttaaagcc
    27661 gtcgaccggg taagtatgac cttaaccgaa ggtgaaatcc gcggtcttgt tggtgaatcc
    27721 ggttccggca agagtttgat tgcgaaagcg atttgtgggg tgaataaaga taactggcgt
    27781 gttactgctg accgtatgcg ttttgatgat atcgatttgc tgcgtctctc cgcacgcgaa
    27841 cggcgcaaac tggttggcca taacgtgtcg atgattttcc aggaaccgca gtcgtgtctt
    27901 gacccttcag aacgtgtggg tcgccagttg atgcaaaaca tcccagcctg gacctataaa
    27961 ggccgttggt ggcagcgttt tggctggcgc aaacgccgtg ccattgaact gctgcaccgc
    28021 gtggggatta aagatcacaa agatgcgatg cgcagttttc cctatgagtt gaccgaaggt
    28081 gaatgtcaga aagtgatgat agccattgca ctggcgaatc aaccgcgtct gctgattgct
    28141 gacgaaccga ccaactcaat ggagccaaca acccaggcgc aaatctttcg cctgctgacg
    28201 cgtctcaacc aaaacagcaa taccactatt ttgcttatca gccatgactt acaaatgctt
    28261 agccaatggg cggataaaat taacgtgctt tactgcggtc aaacagtgga aaccgcgccg
    28321 agtaaggagt tggtgacgat gccacatcat ccttataccc aggcgctgat ccgcgcgata
    28381 ccagacttcg gcagcgcgat gccgcataaa agtcgcctca atacgctgcc cggcgctatc
    28441 ccactgctgg aacagttacc gattggttgt cgtctggggc cacgttgccc gtatgcacaa
    28501 cgagaatgca ttgtgacgcc acgtttgacg ggggcgaaaa atcatctcta tgcctgtcat
    28561 ttcccgctga acatggagaa agagtgagat gatcgaaacg ctgcttgaag tgcgtaattt
    28621 aagtaaaacg ttccgctacc ggaccggctg gtttcgtcgt cagaccgtag aagcggtaaa
    28681 acccttgagc tttacgctac gtgaaggcca gacactggcg attattggcg agaatggttc
    28741 gggtaaatcc acgctggcaa aaatgctggc gggaatgata gagcctacca gcggcgagtt
    28801 attgattgac gatcatccac tgcattttgg cgattattcc ttccgcagtc agcgtattcg
    28861 catgattttt caggatcctt cgacctcgtt gaatccccgt caacgtatct cgcaaattct
    28921 ggattttcca ctgcgcctga acaccgacct ggaaccggaa cagcgtcgca aacagattat
    28981 tgaaacgatg cgtatggttg ggctgctacc ggatcacgtc agttactatc cgcatatgtt
    29041 ggcacccgga caaaaacagc gtctgggtct ggcgcgcgcg ctaatattgc gcccaaaagt
    29101 cattattgcc gatgaagccc tcgcttcact ggatatgtcg atgcgttcgc agttgattaa
    29161 tctgatgctg gagttacagg aaaaacaggg catttcgtat atttatgtta cccagcatat
    29221 cggaatgatg aagcacatta gcgaccaggt gctggtgatg catcagggcg aggttgtcga
    29281 gcgaggcagc accgcggatg tgctggcatc gccgctgcat gaactcacca aacggctgat
    29341 tgccgggcac tttggcgaag cattgacagc ggatgcgtgg cgtaaagatc gctaattttc
    29401 tgaatcctcc ttccagatta tccacctcat tctgacaaga gattaaatct tgtcagaatg
    29461 aggtgatgga taaaataaaa tcaaatgcgc gtgatttaag acgcaatctc actttgcagg
    29521 aacgaaagct ctggcgatat cttcgcagcc gacgttttgg tgatttcaaa tttcgccgtc
    29581 aacatccggt ggggagctac attctcgatt ttgcttgctg ctcggcgcgc gtagtcgttg
    29641 agctggatgg tgggcagcat gatttggcag ttgcctatga taccaggcgc actagctggc
    29701 ttgagtcgca gggctggacc gtgctgcgtt tctggaataa cgagattgat tgtaatgagg
    29761 aggcggtgcc ggagattatt ctgcaggaac tgaaccgccg atcaccctca ccctgaaagg
    29821 gcgagggggc cgaccgagcc gaatagctgt tgtggtgaaa acatggagac agtgctggag
    29881 aatattcggc aaggtctgaa ccatcccagc catcgccata aaagggttag gggctgtatg
    29941 agcctgtttg ttgctggggt aacaatattt gcacaatatg gtcccctcgc ccctctgggg
    30001 agagggttag ggtaagggga aaagcgccca ccctgccgca gcctgctccg gtcggacctg
    30061 gcaactatag ctactcacag ccaggttgat tataataacc gtttatctgt tcgtactgtt
    30121 tactaaaacg acgaatcgcc tgattttcag gcacaacaag catcaacaat aaggattaaa
    30181 gctatgggtt ttctttccgg taagcgcatt ctggtaaccg gtgttgccag caaactatcc
    30241 atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag ctgaactggc attcacctac
    30301 cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg ctcaattggg ttctgacatc
    30361 gttctgcagt gcgatgttgc agaagatgcc agcatcgaca ccatgttcgc tgaactgggg
    30421 aaagtttggc cgaaatttga cggtttcgta cactctattg gttttgcacc tggcgatcag
    30481 ctggatggtg actatgttaa cgccgttacc cgtgaaggct tcaaaattgc ccacgacatc
    30541 agctcctaca gcttcgttgc aatggcaaaa gcttgccgct ccatgctgaa tccgggttct
    30601 gccctgctga ccctttccta ccttggcgct gagcgcgcta tcccgaacta caacgttatg
    30661 ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata tggcgaacgc gatgggtccg
    30721 gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc gtactctggc ggcttccggt
    30781 atcaaagact tccgcaaaat gctggctcat tgcgaagccg ttaccccgat tcgccgtacc
    30841 gttactattg aagatgtggg taactccgcg gcattcctgt gctccgatct ctctgccggt
    30901 atctccggtg aagtggtcca cgttgacggc ggtttcagca tcgctgcaat gaacgaactc
    30961 gaattgaaat aatcattctg ttggtaaata ttgggcggcg ttctgccgcc cgttatctct
    31021 gttatacctt tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt
    31081 caggataacg atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc
    31141 aaaagccact ggtatcatga aacgcaatcc agtactgcgg agtatgacgt tctgcctctg
    31201 gtcccggaag ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagaa
    31261 acgctggctc ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggaattgttc
    31321 cctgaccagc ttaccgtgac ccgttcacag accttcaccg cttatgaacg cttgtctacg
    31381 gccctgacgg ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga
    31441 cttacgccgc tccccgggcc tgattcctcc agggaaagca atcataggtt ggcacaaatc
    31501 acgcaatatg cccgccaact ggctagctcg ccttctatta tcgacaaccg atcgcgccag
    31561 catctgaatg acgtcggtct tactgcctgg gactgtgtga tcattaacca aatcattggt
    31621 tttattggct ttcaggcgcg gaccattgcg acatttcagg cttatctcgg gcacccggta
    31681 cgctggttac ccggtctgga aatacaaaac tacgccgacg cgtcactgtt tgctgatgaa
    31741 tcaatacgct ggcgaagcag ctatgaggtg gaaaaactac ctgaagagta cacaaaaagt
    31801 tcaacagcag aactttgcca actggccgaa acactctctc tccaccctat ttcactttcc
    31861 cttctcgaaa ggttgttaaa cagtacacgg gttaatacac agccggataa taagcttgcg
    31921 gcgttgttat gcgcgcggat aaatggcagt cctgcttgtt ttgccgcctg tatggattca
    31981 tcaaatgaat ataaaaaatc agcccccttc tgcgcaaggg cgaaaatgaa attaaccaat
    32041 gggctgaccg tcattctgtt gagcacgcta ccgttcaggc gatacaatgg ctgacccgag
    32101 cacccgatcg ctttagcgcc gcccagttca gccctttact cgaacacgaa aaatcatcaa
    32161 cgcagattat taatctgctg gtatggagcg ggctgtgtgg ctggataaat cgcttaaaaa
    32221 tcgcgttggg tgagacatat taaccttgcc gcgtcagaca gattcgcgta aaactgtcag
    32281 ccgctctaat ggccaccaaa atagacaatt atgtttcagg acaacccgct gctagcgcag
    32341 cttaaacagc aactgcattc ccagacgcca cgcgctgaag gggtggtaaa agccacagaa
    32401 aaaggctttg gcttcctgga agtcgacgcg caaaaaagtt atttcattcc gccgccgcag
    32461 atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga tccacagtga aaaagaacgt
    32521 gaatccgcag agccagaaga actggttgaa ccgttcctga ctcgtttcgt gggtaaagtt
    32581 cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc cactcttaaa agacgccatt
    32641 ccttgccgcg cagcccgtgg cctgaaccac gagtttaaag aaggcgactg ggcggttgcc
    32701 gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct atgctgaact gacacaatac
    32761 atcacttttg gtgacgacca ctttgtaccg tggtgggtta cccttgcgcg ccataatctg
    32821 gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg atgaaggtct ggttcgtgaa
    32881 gatctgaccg cgctggattt tgtcaccatc gacagtgcca gcacagaaga tatggatgac
    32941 gcccttttcg ctaaggcgtt gccggatgac aaacttcagc tgattgtggc gattgccgat
    33001 ccaaccgcgt ggattgctga aggcagcaag ctggacaaag ccgcgaaaat tcgcgcattc
    33061 accaactatc tgcctggctt caacatccct atgcttcctc gcgagctttc tgacgatctc
    33121 tgctcactgc gcgccaatga agtccgcccg gtactggcat gccgcatgac gctctccgct
    33181 gatggcacca ttgaagataa tatcgaattc tttgccgcca ccatcgaatc caaagcgaag
    33241 ctggtgtatg accaggtttc tgactggctg gaaaataccg gtgactggca gcctgaaagt
    33301 gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt gccaacgccg cggcgagtgg
    33361 cgtcataacc acgcactggt gtttaaagat cgcccggatt accgctttat tctcggtgaa
    33421 aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca ttgccaaccg tatcgtcgaa
    33481 gaagcgatga ttgccgctaa catttgtgcg gcccgcgttc tgcgcgataa gctcggtttt
    33541 ggcatctata acgtgcatat gggctttgat ccggcgaatg ccgacgcgct ggcagcgttg
    33601 ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc tcacgctgga cggtttctgc
    33661 aaactgcgtc gtgaactgga tgcgcaacca actggtttcc tcgacagccg cattcgtcgc
    33721 ttccagtcat ttgctgaaat tagcactgaa cccggtcctc actttggcct cggtctggaa
    33781 gcatacgcca cctggacttc gccgatccgt aaatatggcg acatgatcaa ccaccgtctg
    33841 ctgaaagcgg ttatcaaagg cgaaactgcg acgcgtccgc aggatgaaat cactgtccaa
    33901 atggccgaac gtcgccgtct caaccggatg gcagaacgtg atgttggcga ctggttatac
    33961 gcacgcttcc tgaaagataa agccgggacc ggcacccgtt tcgcggcgga aattgtcgat
    34021 atcagccgtg gcggcatgcg tgtgcgtttg gttgataacg gcgctatcgc ctttattccg
    34081 gcacctttct tacacgctgt gcgcgatgaa ctggtttgca gccaggaaaa cggcactgta
    34141 caaattaaag gtgaaacggt ttacaaagta actgacgtta ttgacgtcac cattgccgaa
    34201 gtccgcatgg aaacccgcgg cattattgcg cgcccggtcg cgtaatctcc tttcacggcc
    34261 cattcctcat ggatgggccg tttatttccc cgctctacct tcgttatatc caggcagatt
    34321 ttttaactga ctttcgtttg aaaactggcg tttttccaaa actggattac aaataaatat
    34381 ataaaaaaac aatgcgttta gccgccgtta atttcatgta agtcatgttg ttccttttat
    34441 ttaaccgcgg actccgcagt taaccggagg atatgcatca tgaaaaccgt tagggagtcc
    34501 acaacgttgt acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc
    34561 gatgttttgc gcttttctac caccgaaacc acagaacctg atcgtacttt gcagttatct
    34621 gccgaacagg ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt
    34681 ctgaccgtcg atgagagcga tctttctgtg catctggtag gacgaaaaat caataaacgg
    34741 gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg cagttgctcg tgatttatca
    34801 cacgggcttt cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc
    34861 gacagccggg ggaatatcca acgcttcaat cggttatgtg aagattacac tgggttgaaa
    34921 gaacacgacg tcattggcca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg
    34981 gcatccaggc gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg
    35041 tggataccaa cacgtaaagg ccagcggctg tttctgtttc gcaataaaat aaatttgtcc
    35101 acagcggcag tggcaaaaac gagatttttt taatctgttc cggtaccgac attaccgaag
    35161 agcgccgcgc tcaggagcga ctgcgtattc tggcaaatac cgacagtatc accggactgc
    35221 cgaatcgtaa cgcaatgcag gagttaatcg atcacgctat taatcaggca gataacaata
    35281 aagttggggt tgtgtatctt gatttggata attttaaaaa ggtcaacgac gcctatgggc
    35341 atttgtttgg tgaccagtta ttacgcgacg tgtcattggc tattttaagc tgtctcgaac
    35401 atgaccaggt gttggcgcgt ccaggtggcg atgagtttct ggtactggca tccaatacct
    35461 cacaaagcgc gctggaagca atggcatcac gaattttgac ccgcttacgc ctcccctttc
    35521 gtattggttt aattgaagtt tataccagct gttcagtagg tatttcgctc tctcccgaac
    35581 atggttcgga cagcgcgact attattcgtc acgccgacac agcaatgtac acagcgaagg
    35641 aaggcggacg aggacaattt tgcgttttta ctccagaaat gaatcagcgg gtatttgaat
    35701 atctctggct ggataccaac ttgcgtaaag cactggaaaa cgatcagttg gttattcact
    35761 atcaaccgaa aatcacctgg cgtggcgaag tgcgcagtct ggaagcacta gtacgttggc
    35821 agtcacctga acgtgggttg attccaccgt tggacttcat ttcctacgcc gaagagtcag
    35881 ggctaattgt gcctttaggc cgttgggtga ttctcgatgt cgtacgccag gtggcaaagt
    35941 ggcgggataa aggcataaac ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg
    36001 atcaaaccat tttcaccgcc ctgaaacagg ttctccagga actcaatttt gaatactgcc
    36061 ctatagatgt tgaactgaca gagagttgtc tgattgagaa tgatgaactg gcactgtctg
    36121 ttattcaaca atttagccaa ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct
    36181 actcttcact ttcgcaactg gcgcgctttc cgatcgatgc catcaaactt gaccaggttt
    36241 ttgttcgaga tattcacaaa caacctgtct cgcagtcact ggtccgggcg atcgtcgctg
    36301 tggcccaggc attgaatctt caggtgatcg ccgaaggtgt agagagtgca aaggaagatg
    36361 cttttttaac caagaacggg atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc
    36421 ccgccgtcgc cttcgaacgc tggtataaac gctatctgaa gcgcgcataa gtagatgaat
    36481 atatatactt agcgctatat cccgtcatta tatatcgtat tcctggatga taattagccg
    36541 ttgctgttaa acacttctct tttagcggca atttttgcat tattcatttt ccacttttgc
    36601 tataaggatg tccttgatgt ctgaatttga tgcccagcgc gttgccgaac gtattgatat
    36661 tgtgttagat atccttgtgg caggcgatta ccactctgcg atccataatc tcgagatact
    36721 taaagcagaa ctcttgcgcc aggttgctga atctacgcca gacatcccga aggcaccgtg
    36781 ggagatttaa caatcgtgcc ttcaggtttt cctatttgag gatgcccggc gcgtcagtat
    36841 ttttttattt agtattataa cgttataaga attacagcga tgaattcccg acaacaaact
    36901 attctacaga tggtcattga ccagggtcag gttagcgtaa ccgatctggc aaaagccact
    36961 ggagtttctg aagttaccat tcgccaggat ctcaacaccc tcgaaaaact gagttacctc
    37021 cgccgtgcac atggctttgc agtttcgctt gatagtgatg acgtcgaaac ccgtatgatg
    37081 agcaactata cgctgaagcg tgaactcgcc gagtttgccg cgtcactggt tcaaccgggc
    37141 gaaaccatct ttatcgaaaa tggcagcagc aatgccctgc tcgcccggac tctgggggag
    37201 cagaagaaaa atgtcactat catcacggtc agcagctaca tcgcgcattt gctgaaagac
    37261 gcgccttgtg aagtcatttt gctcggtggc gtgtaccaga aaaaaagcga aagtatggtt
    37321 ggcccgttga cacgccagtg catccaacag gtgcatttca gcaaagcatt tattggtatt
    37381 gatggctggc aacctgaaac tggattcacc ggtcgcgata tgatgcgtac cgatgtggtc
    37441 aatgccgtgc tggaaaaaga gtgcgaagcg atagtcctga ctgacagctc gaaatttggt
    37501 gctgtacatt catactccat cggtcccgtt gagcgattca atcgcgtgat taccgattcg
    37561 aaaatacgcg ccagcgatct gatgcatctt gagcacagta aactcaccgt tcacgtcgtt
    37621 gacatttaac catttccctt ctctacggat gatttgcagt ttggcaaatc atccgctcta
    37681 agatgattcc tggttgataa ttaagactat ttacctgtta ttaacacgct taagatataa
    37741 aattattatc agcgatataa caggaagtca ttatcacctg cgtgatataa ccctgcgcgc
    37801 gagcagattt cacggaataa tttcaccagg cttattctta gctattatag ttatagagag
    37861 cttacttccg tgaatcataa attcaggaga gagtattatg tttgtaacga gcaaaaaaat
    37921 gaccgcggct gttctggcaa ttactttggc aatgtctctg agtgcctgtt ctaactggtc
    37981 taaacgggac cgcaacaccg caatcggcgc gggtgcaggg gcattaggcg gtgcagtact
    38041 gaccgatggc agtacgttgg gtacattagg tggtgcagct gtcggtggtg ttattggtca
    38101 ccaggtcggt aaataatcgt aaataaatgt cgattatcga ctcgcaatta taatacgctt
    38161 gattctgttc agtacgaaat aactgaataa tacgcacagt aaaatattcg aaagccacgg
    38221 atatatccgt ggctttttat gttaaccgcc tgcgagtttt accttcatcc ctttcgcttc
    38281 aagtagtgat tttaataaat cacgcttatc gccctggatt tcaataactc catctttaac
    38341 tgctccgccg cagccgcatt ttttcttcag ttccgctgca agttttgtca gttcggcatc
    38401 atcgagatcg acaccggtaa tcaggcaaac gcccttacct ttacgtccac tggtctgacg
    38461 ctgaatacgc accacaccgt cgcctttagg gcgtacaggg gccgctttgg gctcatcaat
    38521 acgtccggtt tccgttgagt agaccagacg gctgttggaa tcactcatca tgcactccgc
    38581 tgtaaagagg cgttgatcgc tttcagcgtc tgcgctggat ctaccgattg cgttaccggg
    38641 cgaccaatca ccatataatc aacaccagcc gacaacgcct gttctggcgt cataatgcgg
    38701 cgctggtcac cagcttcact cccctgcgga cgaatgcccg gcgtaaccag tttgaactcc
    38761 tgaccgaata cctgtttaaa gcgcacagct tcctgagcag aacacaccac accatcaagg
    38821 ccacattttt gcgtcagtgc cgccagacgt tctgcataat ctgcaggtga cagtgtcatg
    38881 ccaagatcga ccaggtcgct ggcttccatg ctggtcaaca ctgtcacagc aatcaaaagc
    38941 ggtgcatctt tgccaaacgg aaccagtgcc tcacgcgctg cggtcatcat acgcgcccca
    39001 ccagaggcat gaacattcac catccacacg cctaagtcag ctgcagcagc gacagcgtgc
    39061 gctgcagtgt taggaatatc gtggaatttc aggtcaagaa agatatcaaa accacgctgt
    39121 tgaagttcgc gcacaaactg tggcccaaac aatgtaaaca tctctttgcc gaccttcaga
    39181 cgacaatcgc gtgggtcgat cttgtcgaca aaggacagcg cgtcatcacg attatgataa
    39241 tcaagggcaa caaccacagg agaattcgta acagcgcggg aagaagatga agcagttaac
    39301 gtcatgacca gaccttcttg atgatgggca ccggaagtgg cgcgaaacag gtaaacggcg
    39361 agcattctac ccgtggaaat aaaaaattaa cagttgcgat ttttcccctg gcaggctttt
    39421 tctgctccgt cgttgccgga gcacaatgga acatcaataa ctagtatgtt gtaactaaag
    39481 tcggattttt ttaaaaatta caggccatca agaccgcgaa tcggtttaat ggttgaccag
    39541 gcccgacaag acggacaatg ccagtagagg gtgtatgcgg taaaaccaca tttctggcag
    39601 cgataacgag gcttactacg taccttttcg ccaaccatgt cacgcagcac catcaggctc
    39661 tctttggcac gcccttcttc cgcttcattt aagtggtaat ccattaactt atggaacaca
    39721 cgcatggtcg gatgacgctg aagctggcgc gtaatataga cctgtgcggc ctcactaccg
    39781 tcgcgcgctt cgatgatatc agcaagcatc aattcagcat cggcaccggt gttctcttcc
    39841 accgcgcgtt gcaggaattc tgcccattcg gcagttttac ccaactgctg atagcaggtt
    39901 tgcaacattt ccagcgtttc gctgaccagt tctctgtcct gggatatcac gcgttgcaaa
    39961 ctttcgacgg ctttggcgta ttctcctttc gccataaaca cgcgtcccat catgatggat
    40021 acgcgggcgc tgtttttatc tgccgccgcc ccttttttta gcaaggtcat ggcacgatcg
    40081 agatcgtcgc tggccatatg ctgcagggct aactcacagt agaaatgggc aatttcgacg
    40141 cgctgtttat ctttacccag cttcaccagg cgttcggcaa catcaattgc tttctgccac
    40201 tcgctggtag cctggtagat ttgtagcaac tgttgcagcg cgccaatgcg gaagtcagtt
    40261 tcatcggtca gctgattgaa catgtcttcc gcgcggtcgt ataacccggc ggccatgtaa
    40321 tcacgcccca gttgttgaat cgccaacaga cgctgttcat aggtcagtga ggcgctttcc
    40381 attagggtct gatggatgcg aatagcgcga tcaacttcgc cacgcgaacg gaacaggttt
    40441 ccgagcgtaa ggtgggcttc aacggtgcct gtatcctctt taagcatatc gagaaacagg
    40501 tctaccgctt tatcctgttg attactaagc aggaagttaa cccccgctac gtaatcacgc
    40561 gacaagcggt tggcttcatc ttgcttgttt tgttgcgcac ttctgcggcc catataccag
    40621 ccataggcag cggctacagg caaaagcaga aacaacaact ccagcataga aagttattcc
    40681 ttcgccgctg acgagtgcgg cactacagcc acgtcagtcg cgggtgaaag ctggttttcc
    40741 agtcgcttta ttttacgttc agcgcgcgcc agggaaacac gaactcgcag ccagaacagg
    40801 ccacaaatca accaaccgat agcaaccccc gcagcaaaca ataccgccag caatgtggaa
    40861 atacggtact ccccttgcgc taacagataa ttaaacgtca cctgttgatc gttctgcgca
    40921 cccaacgtga ccgaaatcac gaagatcgct aacaccagta agaaaatgag taaatatttc
    40981 acattacttc ccgttatgtg gttaaagcga ataaattatg gtcaacttac cgcaatagcc
    41041 ctttaaagta ccattttcgc gccgtctgcg aaatggcatc cacgcgcatt gttgatgatt
    41101 taagggctaa attcggaaaa tcaaccagcg ttaactttct tgttctcgtt gcgctatttc
    41161 gcgattttct tccgcaggtg gtgttaatgg cccacaaatt cgttgcgcaa gccacgttgc
    41221 caccgccacc agcgcccacg aaatcaacgt agctactacc agatcgcgtg gccaatgcat
    41281 cccgagcagc aggcggcttc ccatgactcc cgttgcccag accagcaaga tagcaatggt
    41341 taacgttcgc cgacgcggcc acagcaaacc aacggccagc agtgcccaac tggcagcaaa
    41401 catcgtgtga ccggaaggaa aggcaaaccc cgtctctttc tgccagtgtg aacgcaaata
    41461 ttgtgggata tttttctctt cagccaactg ttctttcact agatttccgc gttctgctcg
    41521 ctttaaagtg tagaactcat caaccggaat atgatgtgtt ttttccagcc agataacaaa
    41581 aggtcgtggt tcctggactt tgtctttgat ccaggattta acgccttgtc ccacaatgat
    41641 tacggccgcc agaatggcaa ataatacaaa ggcagcctta atgcgaaaac gcagacacca
    41701 gagaaaccag ccgaataaaa tcaaatgtgt aatgacgccc cagggctggg tgacagtttc
    41761 agtaacccaa aaagccgctt ttagtagcca actttgttct ccaggttgcc aacgccagcc
    41821 agaaatccat acggctactg gcatgacaag caatagtgca gctcccactg cggtacgtct
    41881 ggcaatcgaa cgcatggcct ctcctttttg ataagtccca caatcataac tgaaaacgcc
    41941 agttccagga aaaattgaca gatttgtgcc attccgtgaa cgatcgacgc gtcgtgatta
    42001 ggtgaacccc ttctcgttat ggcaaaataa gccaatacag aaccagcatt atctggagaa
    42061 tttcatgcag cttaaacgtg tggcagaagc caaactgcca accccatggg gcgatttcct
    42121 gatggtggga tttgaagaac tggcaaccgg acacgatcat gtcgcgctag tctatggcga
    42181 tatttccggg cataccccgg tacttgcgcg cgtccattcc gaatgtctga ccggtgacgc
    42241 cctgttcagc ttgcgctgcg attgtggctt ccagctcgaa gcggcattga cgcaaattgc
    42301 cgaggaaggc cgtggtattt tgctgtatca ccgtcaggaa ggtcgtaaca ttggtctgct
    42361 gaataaaatc cgcgcttacg cactgcagga tcaaggttac gataccgtag aggctaacca
    42421 tcagttaggc ttcgccgctg atgagcgcga cttcactctt tgcgctgata tgttcaaact
    42481 ccttggcgtc aatgaagtcc gcttgttaac caataacccg aaaaaagtcg aaattctgac
    42541 cgaagcaggg attaatattg ttgaacgcgt accattgatt gtaggtcgta accccaataa
    42601 cgaacattat ctcaatacca aagccgagaa aatgggtcat ttgctgaaca aataaccctc
    42661 tcgcattgtg gaattaattt gcttgccgga agcaaaataa ccggcaagca aatatttgtt
    42721 acttcagcat attacgaatg acataatgca aaatgccgtc gttctggtag taggtcaact
    42781 ccgtcgcggt gtcgatacga caacggcagg gtacgacctc ctggctacca tccgcgcgcg
    42841 taagcgtcac cggaaccgtc gcaccgggtt gtagattttg cagatcgcca atatcaatct
    42901 tctcttcccc ggttagtcct aacgttttac gcgttacgcc ttgcggaaat tccagcggca
    42961 ggatgcccat gccaattaaa ttcgaacggt gaattcgttc aaacgattcg gcaatcacca
    43021 cacgaattcc aagcagacga ggaccttttg ccgcccagtc acggcttgag cctgaaccat
    43081 actctttccc ggcaatcacc gccagcggcg tttgttcctg cttatagcgc atcgcagcat
    43141 cataaataga gactacgtcg ctgtcaggta aatgccgcgt catcccccct tcaacgccag
    43201 gcaccatttc attacggatg cgaatattgg cgaaggtgcc gcgcatcatc acttcatggt
    43261 taccacgccg cgaaccgtag gagttaaagt cttttcgctc aacaccccga ccttgtagat
    43321 atcgacccgc tgggctgtcg ggcttaatac tgcccgccgg agagatatgg tcagtggtga
    43381 ctgaatcccc cagcattgcg aggatccgcg caccgtgaat atcttccact ggtgctggtg
    43441 ttgcctgcat ttcatcaaag aaaggcgata agcgaatata ggttgagtcc tcctgccaac
    43501 cgtaggtatc ggatcgtgtg acgttaattt ccttccactc tgctgtgcct tcaaaaactt
    43561 ctgcgtactc tttgcggaac atttctgtgg agacttgttc taccgcacgg gcaatttctt
    43621 gtgccgatgg ccagatatct ttcagataaa ccggatcgcc tttacgatca tgaccaatag
    43681 gctcagaagc caggttgata ttcatatttc ccgccagcgc ataggcaacc accagcggcg
    43741 gcgaggccag ccagttggtt ttaaccagcg gatggatacg gccttcaaag ttacggttgc
    43801 cggacagcac tgcaccgacg gttaaatcgc cttttttgat tgccgtttcg ataggatcgg
    43861 gcagcggccc agagttacca atacaggtag tacaaccgta tcccacaagg ttaaacccca
    43921 gttcgtcgag aaacggtgtc agtttcgctt ttgccagata gtcagaaaca actttcgagc
    43981 ctggtgccag cgacgctttg acccacggtt gccgcttgag gccaagaatt acggcttttt
    44041 tcgccagcaa gcctgcagcc atcagcacac ttgggttaga ggtgttggtg cacgaggtta
    44101 tcgcagcaat gaccacagcg ccatcaggta actgatactg atgtccgttc ataacataat
    44161 cgaccggctg gcgatcttta tgcgtggcat tcacttccag ttcgttactg gcggcaaata
    44221 cttttggtac atcgggcagt gcaacgcgat cctgtgggcg ttttggccct gccaggctcg
    44281 cttcaacgtc attcatatcc agttctaacg tactggtaaa aatcggttca tcgcccgggt
    44341 tacgccacat gccctgcgct ttggcatatt tttcgaccaa ctcaacctga tcttcgctgc
    44401 gcccgcttaa gcgcatgtaa tcgagggtta cagcatcgat tgggaagaag ccacaggtgg
    44461 caccatattc tggcgacata ttggcaatgg tggcgcgatc cgccagcggt agtgaatcca
    44521 gaccatcacc ataaaattcg acgaatttct ccaccacgcc atgtttgcgc agcatttggg
    44581 taacagtgag aaccaggtct gtggcggtaa taccttcacg taatttaccg gtaagtttaa
    44641 agcccactac gtccgggata agcatggaaa ccggctggcc taacattgcg gcttctgctt
    44701 cgatcccacc cacgccccac cccagcacgc caaggccgtt gatcatagtg gtgtgcgagt
    44761 cagtaccaac gagtgtatcc ggataagcaa tccattcacc gtcctgcaat tcactccaca
    44821 ctgctttgcc gagatattcg aggttaacct gatggcaaat gcctgtgcct ggcggcacga
    44881 cgctaaaccg actgaacgct tgctttcccc atttcaggaa cacataacgt tcgtggttgc
    44941 gctccatttc caggcgtacg ttttcttcaa atgcctcatc atcaccaaaa cgatcgacgg
    45001 tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg gttaaccttt gcagtatcgc
    45061 cgccgaggcg tttaaccgct tcgcgcattg ccgccagatc aacaacggca ggtacgccgg
    45121 taaagtcctg catcaacacc cttgccgggc ggtaggcaat ttcacggtca gcatgggcat
    45181 ttttcagcca tcctgccagc gcgtggatat cctcttcggt aaccgagtta ccatcctgcc
    45241 agcgcagcag gttttcgagc aaaactttga gtgacttggg tagacgggtg atatcgccca
    45301 gtgatttagc agcaagcggc aggctgtagt agtggtaagt tttatctttg gcctgcaacg
    45361 tgtccttact ggcttctcgt agggttgacg acatagctcc tccttaatga cagggttgcg
    45421 aagccctgat tctcttcagg gtgaatatta aagataacac aaacatcgcg taacgatttg
    45481 ataacaaccc aaattgataa aagaggagaa accttgtggc aaagcaaatg acaaccccgc
    45541 cgcagcgggg tcaaggatca gtgagtaatg atccagagga gttcggccca gaagagcatc
    45601 gacagagaaa acgcgcccat ccaggaccaa tatttcatat ttgtgttgat cataggctac
    45661 acccttaacg gttattattt tgtgttgaga taactcaagc tgttattgct gcggtgtatt
    45721 aaaagccctt taattcatta aaaatgggta ataagaatga tgataattat tgtttcggct
    45781 gatcatcttc agcaaacagt tctataaagg cttgctgctg cgatgttagt tgccagtcgt
    45841 ctggaataac gacggaacat tcccgtttcg tttctgtttc attgtcacta tgctggcata
    45901 atggtgacga ctccagttgc tcctgaccga ccatagttat ccccacactt tccaaatcag
    45961 cagtgcgaca accacccaga aaagtgcaga accaagaaaa accgccagcc aggc
//
LOCUS       DAZPJP010000010        45130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPJP010000010 DAZPJP010000000
VERSION     DAZPJP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.010"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHU9J_000511"
     CDS             complement(<1..264)
                     /locus_tag="ACHU9J_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1904151.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHU9J_000512"
     CDS             complement(424..636)
                     /gene="rpmE"
                     /locus_tag="ACHU9J_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1904152.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            839..3037
                     /gene="priA"
                     /locus_tag="ACHU9J_000513"
     CDS             839..3037
                     /gene="priA"
                     /locus_tag="ACHU9J_000513"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1904153.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRHGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3193..4218
                     /gene="cytR"
                     /locus_tag="ACHU9J_000514"
     CDS             3193..4218
                     /gene="cytR"
                     /locus_tag="ACHU9J_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1904154.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHU9J_000515"
     CDS             4310..5269
                     /gene="ftsN"
                     /locus_tag="ACHU9J_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1904155.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5362..5892
                     /gene="hslV"
                     /locus_tag="ACHU9J_000516"
     CDS             5362..5892
                     /gene="hslV"
                     /locus_tag="ACHU9J_000516"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1904156.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5902..7233
                     /gene="hslU"
                     /locus_tag="ACHU9J_000517"
     CDS             5902..7233
                     /gene="hslU"
                     /locus_tag="ACHU9J_000517"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1904157.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7300..8226
                     /gene="menA"
                     /locus_tag="ACHU9J_000518"
     CDS             7300..8226
                     /gene="menA"
                     /locus_tag="ACHU9J_000518"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1904158.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8319..8804
                     /gene="rraA"
                     /locus_tag="ACHU9J_000519"
     CDS             8319..8804
                     /gene="rraA"
                     /locus_tag="ACHU9J_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1904159.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHU9J_000520"
     CDS             complement(8889..9134)
                     /gene="zapB"
                     /locus_tag="ACHU9J_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1904160.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9560..10405
                     /gene="glpF"
                     /locus_tag="ACHU9J_000521"
     CDS             9560..10405
                     /gene="glpF"
                     /locus_tag="ACHU9J_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1904161.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10428..11939
                     /gene="glpK"
                     /locus_tag="ACHU9J_000522"
     CDS             10428..11939
                     /gene="glpK"
                     /locus_tag="ACHU9J_000522"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1904162.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12069..13079
                     /gene="glpX"
                     /locus_tag="ACHU9J_000523"
     CDS             12069..13079
                     /gene="glpX"
                     /locus_tag="ACHU9J_000523"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1904163.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13176..13922
                     /gene="fpr"
                     /locus_tag="ACHU9J_000524"
     CDS             13176..13922
                     /gene="fpr"
                     /locus_tag="ACHU9J_000524"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1904164.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHU9J_000525"
     CDS             complement(13927..14355)
                     /gene="uspD"
                     /locus_tag="ACHU9J_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1904165.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14382..14681)
                     /locus_tag="ACHU9J_000526"
     CDS             complement(14382..14681)
                     /locus_tag="ACHU9J_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1904166.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14893..15333)
                     /locus_tag="ACHU9J_000527"
     CDS             complement(14893..15333)
                     /locus_tag="ACHU9J_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1904167.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15434..16033
                     /locus_tag="ACHU9J_000528"
     CDS             15434..16033
                     /locus_tag="ACHU9J_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1904168.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHU9J_000529"
     CDS             16141..16908
                     /gene="tpiA"
                     /locus_tag="ACHU9J_000529"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1904169.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHU9J_000530"
     CDS             complement(16963..17718)
                     /gene="cdh"
                     /locus_tag="ACHU9J_000530"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1904170.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHU9J_000531"
     CDS             complement(17825..18814)
                     /gene="sbp"
                     /locus_tag="ACHU9J_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1904171.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHU9J_000532"
     CDS             complement(19134..20096)
                     /gene="pfkA"
                     /locus_tag="ACHU9J_000532"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1904172.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHU9J_000533"
     CDS             complement(20277..21179)
                     /gene="fieF"
                     /locus_tag="ACHU9J_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1904173.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHU9J_000534"
     CDS             complement(21414..21632)
                     /gene="ogrK"
                     /locus_tag="ACHU9J_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR1904174.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21714..22877)
                     /locus_tag="ACHU9J_000535"
     CDS             complement(21714..22877)
                     /locus_tag="ACHU9J_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1904175.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22877..23356)
                     /locus_tag="ACHU9J_000536"
     CDS             complement(22877..23356)
                     /locus_tag="ACHU9J_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904176.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23371..25818)
                     /locus_tag="ACHU9J_000537"
     CDS             complement(23371..25818)
                     /locus_tag="ACHU9J_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1904177.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25811..25966)
                     /locus_tag="ACHU9J_000538"
     CDS             complement(25811..25966)
                     /locus_tag="ACHU9J_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1904178.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25963..26238)
                     /locus_tag="ACHU9J_000539"
     CDS             complement(25963..26238)
                     /locus_tag="ACHU9J_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1904179.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26295..26813)
                     /locus_tag="ACHU9J_000540"
     CDS             complement(26295..26813)
                     /locus_tag="ACHU9J_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1904180.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26826..28016)
                     /locus_tag="ACHU9J_000541"
     CDS             complement(26826..28016)
                     /locus_tag="ACHU9J_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR1904181.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28427..29845
                     /locus_tag="ACHU9J_000542"
     CDS             28427..29845
                     /locus_tag="ACHU9J_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904182.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29960..30487)
                     /locus_tag="ACHU9J_000543"
     CDS             complement(29960..30487)
                     /locus_tag="ACHU9J_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1904183.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30491..32809)
                     /locus_tag="ACHU9J_000544"
     CDS             complement(30491..32809)
                     /locus_tag="ACHU9J_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1904184.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32820..33350)
                     /locus_tag="ACHU9J_000545"
     CDS             complement(32820..33350)
                     /locus_tag="ACHU9J_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1904185.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33343..34251)
                     /locus_tag="ACHU9J_000546"
     CDS             complement(33343..34251)
                     /locus_tag="ACHU9J_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1904186.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34256..34603)
                     /locus_tag="ACHU9J_000547"
     CDS             complement(34256..34603)
                     /locus_tag="ACHU9J_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1904187.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34600..35235)
                     /locus_tag="ACHU9J_000548"
     CDS             complement(34600..35235)
                     /locus_tag="ACHU9J_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1904188.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35302..35754)
                     /locus_tag="ACHU9J_000549"
     CDS             complement(35302..35754)
                     /locus_tag="ACHU9J_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1904189.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35747..36214)
                     /locus_tag="ACHU9J_000550"
     CDS             complement(35747..36214)
                     /locus_tag="ACHU9J_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904190.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHU9J_000551"
     CDS             complement(36177..36422)
                     /gene="lysC"
                     /locus_tag="ACHU9J_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR1904191.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHU9J_000552"
     CDS             complement(36322..36747)
                     /gene="lysB"
                     /locus_tag="ACHU9J_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR1904192.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36735..37160)
                     /locus_tag="ACHU9J_000553"
     CDS             complement(36735..37160)
                     /locus_tag="ACHU9J_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR1904193.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37175..37672)
                     /locus_tag="ACHU9J_000554"
     CDS             complement(37175..37672)
                     /locus_tag="ACHU9J_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR1904194.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37672..37953)
                     /locus_tag="ACHU9J_000555"
     CDS             complement(37672..37953)
                     /locus_tag="ACHU9J_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1904195.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37957..38160)
                     /locus_tag="ACHU9J_000556"
     CDS             complement(37957..38160)
                     /locus_tag="ACHU9J_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1904196.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38160..38669)
                     /locus_tag="ACHU9J_000557"
     CDS             complement(38160..38669)
                     /locus_tag="ACHU9J_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1904197.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38769..39512)
                     /locus_tag="ACHU9J_000558"
     CDS             complement(38769..39512)
                     /locus_tag="ACHU9J_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR1904198.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39516..40588)
                     /locus_tag="ACHU9J_000559"
                     /pseudo
     CDS             complement(39516..40588)
                     /locus_tag="ACHU9J_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40647..41501)
                     /locus_tag="ACHU9J_000560"
     CDS             complement(40647..41501)
                     /locus_tag="ACHU9J_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1904199.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41675..43447
                     /locus_tag="ACHU9J_000561"
     CDS             41675..43447
                     /locus_tag="ACHU9J_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR1904200.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43447..44481
                     /locus_tag="ACHU9J_000562"
     CDS             43447..44481
                     /locus_tag="ACHU9J_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1904201.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44853..>45130
                     /locus_tag="ACHU9J_000563"
     CDS             44853..>45130
                     /locus_tag="ACHU9J_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1904202.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIES"
BASE COUNT        10520 a        12228 c        11669 g        10713 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttc gcaccgagca gcggattgac
      121 cggatggccg gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctggacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgccgcc ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc
      301 ctagcgccag tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt
      361 tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg
      421 gtcttatttg ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt
      481 agcaacatca cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt
      541 caggtcatga ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt
      601 aatttcttcg tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc
      661 gtcgctcttc cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg
      721 atcatttcgt acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc
      781 gcactcttct acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat
      841 gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc
      901 agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga
      961 gcgcatcggg gttgtggtat cagttagcga tgtcagtgag ctgccgctta acgagctgaa
     1021 agcggtagtt gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcgattact
     1081 gctctgggcg gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc
     1141 gattttactg cgccatgggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac
     1201 tgaacaaggc caggcggtgg atctgaacag cctgaaacgc tcacccaagc aacaacaggc
     1261 gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag gttgccacgc tcgaatttaa
     1321 tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc
     1381 agagtttagc gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac
     1441 cgaacaggcc accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct
     1501 gctggcgggc gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt
     1561 gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac
     1621 tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa gttctgcatt ccggtctgaa
     1681 cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat ggcgaggcgg cgattgtgat
     1741 cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgacga
     1801 agagcacgac agttcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc
     1861 ggtgtatcgt gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct
     1921 ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc
     1981 ggggaatgcg cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc
     2041 aggtctggct ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt
     2101 tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta ctgtgccacg actgcggctg
     2161 gattgccgaa tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt
     2221 gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc
     2281 cacgcacctg gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct
     2341 gttccctgat gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct
     2401 ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat
     2461 gctggcgaaa ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg
     2521 cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc gctcagcttt acacccaggt
     2581 cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc
     2641 ggaacatcct ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc
     2701 gctggctgag cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc
     2761 ggaagatcat aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct
     2821 ctccagcccg ctggcagacg acaaattgtg ggttctcggt ccggttccgg cactggcacc
     2881 taaacgtggc ggtcgctggc gctggcagat attgttgcag cacccttccc gcgtgcgctt
     2941 gcaacacatc attaacggta cgctggcact catcaataca ataccggatt cccgtaaggt
     3001 gaaatgggtg ctggatgttg atccgattga gggttaaacc gctcacgatg cgaggcggat
     3061 cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta
     3121 tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg
     3181 cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc
     3241 ctcaaggcaa aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc
     3301 tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag
     3361 cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat
     3421 atctgcgatc ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac
     3481 ggatatctgg tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc
     3541 gatttgatca tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt
     3601 gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca
     3661 ccggaactgg agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta
     3721 aattatctat atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg
     3781 ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg
     3841 gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg
     3901 cagcagctgc ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg
     3961 gcgctcggcg cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc
     4021 ataatcggtt ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc
     4081 gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag
     4141 gggcaacacg ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca
     4201 acacgcgcgt taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg
     4261 cttaagtaac atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg
     4321 agattatgta cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa
     4381 aaagcaacga aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgtgct
     4441 tgtgaccttt atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac
     4501 gctgcaaagc cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg
     4561 ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc
     4621 cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca
     4681 aatgcaggct gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca
     4741 gacgccggaa cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga
     4801 gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg
     4861 tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca
     4921 gcagccgtac caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca
     4981 gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga
     5041 cgaacgccgc tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt
     5101 acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa
     5161 tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt
     5221 gaagatggcg ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa
     5281 tcccccccat ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat
     5341 tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg
     5401 gtcatcgctg gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa
     5461 aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat
     5521 gcttttacgc tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc
     5581 aaagccgccg ttgagctggc aaaagactgg cgtaccgatc gcatgctgcg caaacttgaa
     5641 gcactgctgg cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg
     5701 gtgcagccag aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg
     5761 gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg aaattgctga aaaggcgttg
     5821 gatattgcag gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc
     5881 tacaaagcgt aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg
     5941 gataagcaca tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac
     6001 cgctggcgtc gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc
     6061 ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg
     6121 gcgaatgcgc cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctatgtcggt
     6181 aaggaagtgg attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc
     6241 caggctatcg agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac
     6301 gtgctgatcc cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc
     6361 gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc
     6421 gagatcgatc ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa
     6481 gagatgacca gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg
     6541 cgtaagctga aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg
     6601 gtgaacccgg aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg
     6661 tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct
     6721 cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa
     6781 cacgggatgg tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg
     6841 aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc caatccgcgt tgaactgcag
     6901 gcactgacca ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg
     6961 cagtacaaag cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt
     7021 aagcgcatcg cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt
     7081 cgtttacaca ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta
     7141 agcggtcaaa atatcactat tgacgcagat tatgtgagca aacatctgga tgcgttggtg
     7201 gcagatgaag atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt
     7261 tgatggggct gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc
     7321 cgaactcagg cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca
     7381 attatcgtcg ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg
     7441 ctggtactaa ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc
     7501 gatgcggtaa aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa
     7561 ggggtcatta cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt
     7621 ctctccgggc tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc
     7681 ctgattcttg gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct
     7741 tatggttata tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagcgtc
     7801 atggggagct ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc
     7861 gcctgtggcc tgctggcaac ggcagtactg aacattaata acctgcgtga tatcaatagc
     7921 gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt
     7981 tatcatgcct gcctgctgat gggctctctg gtatgtctgg cgctgtttaa tctcttttcg
     8041 ctgcatagcc tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc
     8101 cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc
     8161 aagggagcgt tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca
     8221 gcataactga caaatatcaa ttaacaattg atgattttgc caacagccta catagcgcga
     8281 tatactgaaa attctcgcag caactgaatg ttaagcctat gaaatacgat acttccgagc
     8341 tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg
     8401 gacgggcgtc gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc
     8461 tgtacgatct gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt
     8521 ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg
     8581 aaggtctggt tatttacggc gcggtgcgtc aggtagatga cctagaagag ttggatatcg
     8641 gcatccaggc gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg
     8701 atgtccgcgt caattttggc ggtgtaacct tcttctccgg cgaccatctt tatgccgaca
     8761 ataccgggat tattctttca gaagatccgc tggatattga atgataagaa aggcaccgca
     8821 aggtgccttt tttctgcgtt acctgttggc ctacacagta aagaaattac gcgaaagatg
     8881 aagcgtaatc agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg
     8941 ttctgctgtt ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca
     9001 ttttgaactt cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc
     9061 aacagagtga tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct
     9121 aatgacattg tcatacctct cctgaattgc aaggcgttga tggataaaaa atcctcgtcc
     9181 cgattaccgg tgacgcctta ataaatacga gcgcacttta gttagctccg attgtatgaa
     9241 gccgcgccat cgctgtccag cggcacgcct tgcagattac ggtttgccac acttttcatc
     9301 cttctcctgg tgacataatc cacatcaatc gaaaatgtta ataaatttgt tgcgcgaatg
     9361 atctaacaaa catgcatcat gtacaatcag atggaataaa ttgcgcgata acgctcattt
     9421 tatgacgaag cacacacatt ttaagttcga tatttctcgt ttttgctcgt taacgataag
     9481 tttacagcat gcctacaagc atcgtggagg tccgtgactt tcacgcatac aacaaacatt
     9541 aactcttcag gatccgatta tgagtcaaac atcaaccttg aaaggccagt gcattgctga
     9601 attcctcggt accgggttgt tgattttttt cggtgtgggt tgcgttgcag cactaaaagt
     9661 cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt tggggactgg gggtggcaat
     9721 ggccatctac ctgaccgcag gggtttccgg cgcgcatctt aatcccgctg ttaccattgc
     9781 attgtggctg tttgcctgtt tcgacaagcg caaagttatt ccttttatcg tttcacaagt
     9841 tgccggcgct ttctgtgctg cggctttagt ttacgggctt tactacaatt tatttttcga
     9901 cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa agtgttgatc tggcaggcac
     9961 tttctctact taccctaatc ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt
    10021 gattaccgct attctgatgg ggctgatcct ggcgttaacg gacgatggca acggtgtacc
    10081 acgcggccct ttggctccct tgctgattgg tctactgatt gcggtcattg gcgcatctat
    10141 gggcccattg acgggttttg ccatgaaccc agcgcgtgac ttcggtccga aagtctttgc
    10201 ctggctggcg ggctggggca atgtcgcctt taccggcggc agagacattc cttacttcct
    10261 ggtgccgctt tttggcccta tcgttggcgc gattgtaggt gcatttgcct accgcaaact
    10321 gattggtcgc catttgcctt gcgatatctg tgttgtggaa gaaaaggaga ccacaactcc
    10381 ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca attaaacatg actgaaaaaa
    10441 aatatatcgt tgcgctcgac cagggcacca ccagctcccg cgcggtcgta atggatcacg
    10501 atgccaatat cattagcgtg tcgcagcgcg aatttgagca aatctaccca aaaccaggct
    10561 gggtagaaca cgacccaatg gaaatctggg ccacccaaag ctccacgctg gtagaagtgc
    10621 tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat cggtattacg aaccagcgtg
    10681 aaaccactat tgtctgggaa aaagaaaccg gcaagcctat ctataacgcc attgtctggc
    10741 agtgccgtcg taccgcagaa atctgcgagc atttaaaacg tgacggttta gaagattata
    10801 tccgcagcaa taccggtctg gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga
    10861 tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg tggtgaattg ctgtttggta
    10921 cggttgatac gtggcttatc tggaaaatga ctcagggccg tgtccatgtg accgattaca
    10981 ccaacgcctc tcgtaccatg ttgttcaaca tccataccct ggactgggac gacaaaatgc
    11041 tggaagtgct ggatattccg cgcgagatgc tgccagaagt gcgtcgttct tccgaagtgt
    11101 acggtcagac taacattggc ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg
    11161 gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca
    11221 cctatggcac tggctgcttt atgctgatga acaccggcga gaaagcggtg aaatcagaaa
    11281 acggcctgct gaccaccatc gcctgcggcc cgactggcga agtgaactat gcgctggaag
    11341 gtgcggtgtt tatggcaggc gcatccattc agtggctgcg cgatgagatg aagttgatta
    11401 acgacgccta cgattccgaa tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg
    11461 tggttccggc ctttaccggg ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga
    11521 ttttcggtct gactcgtggg gtgaacgcta accacattat acgcgcgacg ctggagtcta
    11581 ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc cgactctggt atccgtctgc
    11641 acgccctgcg cgtggatggt ggcgcagtag caaacaattt cctgatgcag ttccagtccg
    11701 atattctcgg cacccgcgtt gagcgcccgg aagttcgcga agtgactgca ttgggcgcgg
    11761 cctatcttgc tggtctggcg gtcggtttct ggcagaacct cgacgagctg caagagaaag
    11821 cggtgattga gcgcgagttc cgtccaggca tcgaaaccac tgagcgtaat taccgttacg
    11881 caggctggaa aaaagcggtt aaacgcgcga tggcgtggga agaacacgac gtaatgtaaa
    11941 tgccggatga agcgtttatg ccgcatccgg tagtcccgaa acgtgcgggg gcaaccccgc
    12001 acacatcaat aatccctccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
    12061 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tctgcggcgc
    12121 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
    12181 acgccatgcg tattatgctc aaccaggtca atattgacgg caccatcgtc attggtgaag
    12241 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
    12301 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggccagg
    12361 ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat gcgccggata
    12421 tgtatatgga gaagctgatc gtcggaccgg gagccaaagg caccattgat ttgaacctgc
    12481 cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg caaaccgttg agcgaactga
    12541 cggtaacaat tctggctaaa ccacgccacg atgccgttat cgctgaaatg caacaactcg
    12601 gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc acctgtatgc
    12661 cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc gtagtttctg
    12721 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
    12781 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
    12841 cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc agcgataacg
    12901 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
    12961 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc attcgccgca
    13021 ttcagtctat ccactatctg gatcgcaagg acccggaaat gcaggtgcac atcctctgat
    13081 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
    13141 aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
    13201 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccccgtgc
    13261 ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc gaacgcgtcc
    13321 agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
    13381 ccgtccccga tggcaaatta agcccacgac tggcggcact gaaaccaggc gatgaagtgc
    13441 aggtggttag cgaagcagct ggcttctttg ttctggagga agtaccagat tgcgaaacgc
    13501 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggca
    13561 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
    13621 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa ctgcgcattc
    13681 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata ccggcattaa
    13741 ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
    13801 tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg aaagagaccc
    13861 ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag cattactggt
    13921 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct tattgatcat
    13981 cccgcgatat gccggcatca aacggttgat aaatgagtgg tgatgaccac agacaaggag
    14041 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
    14101 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
    14161 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
    14221 ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
    14281 ctccagggct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
    14341 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
    14401 gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc atcaagctca
    14461 acttgcccct ccagatcctt aatcttatag gccacgctgg ccccttcatc tgcagaggat
    14521 gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc gcgggtggca
    14581 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
    14641 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
    14701 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
    14761 tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc agaaggattt
    14821 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
    14881 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
    14941 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
    15001 caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac ccggtaaaat
    15061 cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc atgcgccgga
    15121 acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa gcaagcacac
    15181 aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg agaaaagcac
    15241 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
    15301 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
    15361 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
    15421 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
    15481 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
    15541 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
    15601 gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
    15661 tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag cgctggagcg
    15721 cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg ggccagagca
    15781 aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca cactcacccc
    15841 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctactaacgg cggggaaaaa
    15901 caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg acaacggcga
    15961 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
    16021 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
    16081 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
    16141 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
    16201 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
    16261 gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag ccacatcatg
    16321 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacttctgcc
    16381 gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga acgtcgtact
    16441 taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcagggc
    16501 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
    16561 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
    16621 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
    16681 gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt tgacgctaac
    16741 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
    16801 ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct gaaagctgac
    16861 gctttcgcag tgatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
    16921 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
    16981 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
    17041 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
    17101 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
    17161 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
    17221 caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca catcaggacg
    17281 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
    17341 caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa aatcacgcgc
    17401 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    17461 accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa gatcttttaa
    17521 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    17581 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    17641 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    17701 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    17761 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctggg
    17821 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    17881 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    17941 tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    18001 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttggtgcctt
    18061 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    18121 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    18181 cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg gagccgcgcg
    18241 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    18301 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag ttccagcgtg
    18361 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta atcagatcgt
    18421 tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg gtggaagtgt
    18481 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    18541 caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    18601 ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc acgttatcac
    18661 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    18721 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg gttgccgcca
    18781 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    18841 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    18901 aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc atattgctga
    18961 caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc tacaaaagtt
    19021 tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc atcaggcatt
    19081 tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa tcattaatac
    19141 agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt ttcgatagcg
    19201 tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg accgccgtaa
    19261 cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat acggtcgtaa
    19321 ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt ttcacgaccg
    19381 gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc ggtaatcgcc
    19441 acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac caggtcttca
    19501 cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat ggccgcagcc
    19561 aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg ctggtgagaa
    19621 gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc agtgaagaaa
    19681 ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag accgatgcac
    19741 gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc gccgataacc
    19801 accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg gatgttctcg
    19861 tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt gatcatgtca
    19921 gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag atagccgtca
    19981 taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc gcgaattgcg
    20041 gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt aatcatgact
    20101 acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt gcacatcgaa
    20161 gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg attcaggtca
    20221 ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta tactgattat
    20281 gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg atggataatt
    20341 acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc caccatatgt
    20401 gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa gcgggtcggc
    20461 cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga agtcacgata
    20521 tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga ctgtaccgcc
    20581 tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa tgcaaacaga
    20641 gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat tgcgccgttc
    20701 atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct ttgcgtccgg
    20761 cgcaccaccc accgctgaaa cgagacaagg ataatcgtac aaattagcgc cacaattgtc
    20821 acgataaccc cgacgcctgg atctgtcatc ggtgttggag ataccagatg ttgaataccc
    20881 gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag cgccgcgagg
    20941 gactcagctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag ggaatatcgc
    21001 accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac cagcgcggcg
    21061 agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa tagcagcgaa
    21121 gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga ttgattcata
    21181 aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc aatgcattaa
    21241 gcagcaggca aattgaggat aaaaaaaccc ccacatcatg tgggggaaga cagggatggt
    21301 gtcacaaaaa agtacctaac ctgatgatat aaatggattt atgtcaaccg ctgtccacat
    21361 agcgtccaca tcgaccataa ataagcccct caactgaggg gctatttttt tgatcacatc
    21421 cacataattt gctgccctga cggcaacggg tgcggcctta cggcgtggac ttctcccggc
    21481 ttcacgatgt atcgctgtac cgactcataa gtgatgaacg tggcgctgca attcacgttc
    21541 tggcactggt gataacgctc ttttgtcgtg tcagtgatat agcgacttgt acgcgcatgt
    21601 gcggcatgct ggcataaagg acaatgaaac atcgcgagca cctcttccgg ttttgttgat
    21661 ggtgccattt tagttaattt atccttataa aacaaacaga taaataaacc acgtcactca
    21721 tcatcttctg tttcgtactc cacatcagaa agccttacct caagctccaa ggacgtcgtg
    21781 aagccgctat tattgagaca atgtgtcacc ttagtgatgg tccagtcctg ctcgtctatg
    21841 acgcgcttaa agcctgacac tttaaccggt gtttccgtgt aaatatctgc acgaccggta
    21901 gccaggctga tggagaactc cgctacaccc cgttgcagtt tatcccactt cgcctgagcg
    21961 gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca gggcaaaaac gttgtcagct
    22021 tcaccggcca tgtattcacc ttcgcgcgct tccggtactt ttggcgcttt cttctgcgtg
    22081 accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt tttctttctt tttgcgtttc
    22141 agttttacct tctgcttttg cggcttcggg tctttggtgt gtaaccactt tgccgttacg
    22201 ccggtgtaag ctccacggtc agcaatcgca aaatgatgac ggtcgccgtc gctgcgggtg
    22261 atggtaatct gcgggatttt tttaccgctg gccgtcaccc cctgccccgc tttgagaaac
    22321 agcagttttc ccatttttac cgacacctcg ccaccgttgc gttcagcaag acgggtcagg
    22381 aatttcgcat cagactcctg cgactggtcg atgtgtggaa tttttattcc ggccagtgac
    22441 ggagcgacac tggcttccag cctgttacgg gaggctatcg cctcaacaat cgcaccgagc
    22501 gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg tcccgcgaaa atctgcactc
    22561 cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt caacctcatc aacggtaaat
    22621 ttccctttgc ataccagggc aaaacctttc cagccgatat acaccgtcag gacagcgcca
    22681 cgaaccggca gcccgacctg cccgtcggca tcgttcagtt caatatcaag ctggtcagcc
    22741 tcaaagcccc ggttatccgt cagggtcatg ctcatcagac ggtcgctgat attgccggta
    22801 atatccctgc tgtcgagcat cagcatgtaa tccggcgtca gcgtactgcc tgcatcaaat
    22861 gtcagcgcat ccagcattat cccgcccccg tcatacccgt gaatttagtc gccatactgc
    22921 cagccttacc gatgagcgat tccgcctgtt taccgatatc gccataaagc gcggccagtg
    22981 actcatcaac gcgggtgagc gacagcgtaa aatcaatttt ccgtggtgtg ccgtctgcaa
    23041 agaaaatact ccctgtttca ctcaccttgc tgatgacata catgccgtaa atcatgccgg
    23101 tgccatccag caacggccac gcccggcctt cctctgccat cagtctgagc gtggtcatcg
    23161 tcagctttcc gccagtcagt tcgggataaa gcacaccggc aagtgtgatg ttctcctcac
    23221 ctacaccgag aaactgatag gcatcccgtt taccgatacg ggaatttgac ggccagcgat
    23281 aatctgattc acgctgcatg gtctggtgtg gcagcgtctg gcgcataaaa acaaacatac
    23341 ctaacgcgag catcattttt cgtcacctcc ttaaccgtca tgcatcatgc tggcacgggc
    23401 gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc tgtaactggc ggtcaagctg
    23461 tgtccccggt gcagtaccac ccgtcaggct gatgtgatat tcgtttttac tctggtccac
    23521 ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc tgatagcctg cataagagct
    23581 ggtcgccgga atataaccac cggtgccata cgtggcggca tgagttctgg cggcggtctg
    23641 gtcaagtgtg tctgactctt tgttgatgac cccgagcttt tccagtaccc agtcaatgcc
    23701 gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc gcgtcagcca gtgcctgccc
    23761 gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc tgggtggctt tgaccggggc
    23821 aatcaggttt ttaaaccact gccacgcagc ctgtaacttt tcgcccagcc agtcaaacac
    23881 cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat gccgctttca gcccttcaac
    23941 cacaccgcca aagaatgcgc tgacaggctc ccagtattta cggataagca acgccccggc
    24001 gacaatggcg gccaccacag ccacaaccgg ccagctaatc gccccgatgg ccgtcataac
    24061 ggcactacca accgtcgtga agattgcccc cattgcgcct gctgccgcga tgatggcatt
    24121 gatgccggtg ataaccggcc aggctacaag accaattgca ccgatgatgc cagtcagcgc
    24181 cagcgcgcca ccggcaatga ggccaatggt tgacgccagt gatttgtttt tctgtaccca
    24241 gccgtcgagt tttaacacat actttgtggc cgtctgcgtg agcttacgta gcgcgccttc
    24301 ctgctggtca aacaggtctg tccccaccgc ctcataagcg gactgaaact ccttaaagtc
    24361 accgccgagg ttgtcctgca tgatatttac cagctctgcg gtcttcccgt ctgaggcttt
    24421 aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg gcagtcatca gcacagcggc
    24481 ggctgagctg gcctcctccc cgaaaatggt tttcatgtat tcggcctgct gggcagtacc
    24541 gagccggttt ttctcaaagc tggcctgcat ttctttcaga atggtaaata ctggtcgggt
    24601 gtttccctta ctgtctgagg ttttcacgcc aagctctttg agtgcatccc atgcttttcc
    24661 agtcggtgcc tgcaggcgac ttaacacggc acggcttccc gtccccgcca ttgagcctgt
    24721 gatttttgca tcatgcagcg ccccgaccat tgcggcggtt tcttcaatgc tgacaccggc
    24781 attttttgcc actggtgcgg cataggtcag cgcatcgctc atgccgtcaa aatcagcggc
    24841 ggttttgttc atcgtcatgg agagaacatc cccgatatga gcgaccttat cgtttgaaag
    24901 ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt tcttccattg tgcggcggtt
    24961 cgccagcgcc atattcagcg tgaccggcgt tgccgcctga atggcatcaa catccccacc
    25021 cgctttcgca atgataatct gtgcaccggc cgcatcatcc gctgaggcgg cggtgttgtc
    25081 accgagctgg cgcgcctgct tgcggagtgc agccatttcg gcggagtctt ttgccacacc
    25141 gagcacagcc tgcaattctg agtttttctg cgcaaactca taacctggca tcagcagctt
    25201 aactccggct atcgttcccg cagcagcaat ccccacaccg gcagcgccca ctgaggccat
    25261 atttccggcc agttcctttc cggcctgata acgctgtttg actgcgttaa gttttgcctg
    25321 ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta agctgggcgg tggtttcact
    25381 gatacggttt ttcagtccct gctcatcatg tgcaagattg cgggtattaa ttcccacagc
    25441 ggccagttcc cgctgctggc gtttaacgga atctgtcagg cggttatatt tcgcctgtaa
    25501 gtcctccgcc gcacgctttg cggattccag cactttcgcc tgagcacggg tcgggcgttc
    25561 ggtgttttta aactgtgtgg caagggcttc ggcctcctgc cgtgcctttt caagtgcatg
    25621 accagtcacg gcgagctgtg cactggtctt gcggaatccc tcaatacggg atgcgtgacc
    25681 gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc cccgacagca acttactcgc
    25741 tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca gccctgagca atacctgtaa
    25801 ttttacattg ttactcattc gtgtttccgc ttcgcctgag cgccttttcg cgccatgtga
    25861 tgagttcggt caggctcatg ggatacagtt ctgatggcgg ccagtgaaat atcactgcca
    25921 catccgccat caggtcatcg accgacagat ttttcggaag cgtcactgca ccgagttcgg
    25981 cgacaaaaaa ccgaccacct taccggccag cgccacaagg tcaggcagtt ccagcgcggc
    26041 gacttcctgc tcggtcagca tcggtgccgt catgcgcggc agcaccttaa tcagtgcatc
    26101 gacttctgag tttgcgaccg cagccagact gacaccgcgc agcgtccccg cactgggttt
    26161 catcagcgtg acctgttcga taacctgctc accacgcttg accggattgt ccagggtaat
    26221 cacattttct ttgttcatgg ttttctcact tctgaatcgg ggttaaccgg tcagccaggc
    26281 tgaccggatg aaaattacag gccgatattg cggcggtgtt gctccagccg gtcgacgccg
    26341 ttcaccttct caatcatgtt gatggtgtcg atttcgacca gctccttacc gtccatcgtc
    26401 agccggaaat aggtgcagac cacggagatt ttcgactcgg tgtcttctcc ctgtttaccc
    26461 tcgccggtgt cgatttcttt ctgacggcca cgcatgacca cctctacggc caccgtttcg
    26521 ccggtatcgt cacgctggta agagcctgca aaacgaatcg gcacggcatc cacaccggtt
    26581 gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc cgagcgacca ctccattgac
    26641 agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc cgttcatccc cgcaccgcga
    26701 tagttttcga gtttacgggt cagttttggc agcgtgacgg actttgcaac gccctgatag
    26761 ctgtagccgt tcagaaagac gttcattaac ttgagtttgc gcggcattgc catcggtcag
    26821 gctccttaat tgctgttaac cgaggtgacc agatttgcca ggtatttatc ggtaatacgc
    26881 tggcgcaggg tcaggttttc aagaggaggc accggggtat agtcgtagtc gatatacagt
    26941 tttccggcct tgagggtttc cgcatcgttg gattcttcgc tgaaccagca ggtcgcatcc
    27001 acgatatagc cgtttgtttt cagctcacgg aatttggcat tgatgccgtc aacgatgtcg
    27061 cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca tgtgcgcctc agccatcgtg
    27121 tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga ggaacagcgg gtcatcagag
    27181 caggtacggt taccccagaa gcggaaaccg tcacggcgaa tcagcgttgt gacgcctgac
    27241 tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat cccagaatac ggacgcgctg
    27301 atgccggtaa caccgttcac cccgacgttg gacagcgttt tatgccagcc ctgctcctgg
    27361 tcgattttag cgcgcagacc cagcgcacgg gcggtggcat acgcggtggc ggtggtactg
    27421 gcgaccgtat cccatgcgag gaaatccggc cagatgacca tcagctcacg ctggctgaaa
    27481 ttctgacggt aggctttcac ctcggaaacg gtcttacagc cccatgcgct gatatacccg
    27541 aaagcgcgca gcttctgaca gactgatgcc agtgcaacag ccacctcttt ggtgtccagt
    27601 cccggcacgc cgagaatacg cggtttaaca ccggttaccg actccgccgc cagcagggct
    27661 ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga tgatattgga aacggtctgc
    27721 gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca cacgcacgac aacggtgacc
    27781 ggttttgact ggtcggcgat ggcctgcaac gatgccgcca gcgtgccttt tttaccggcc
    27841 tttgcaattg cgctctgcac attggtaatc agcaccggtt tattgagggg gaaggtttcc
    27901 gcatccgcat cgctggccgt gcagaccatg ccgacaatgg cagtggatac ggtggaaatg
    27961 acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt gatgatagtc actcatccgt
    28021 ttaactccgt ggttaatggg tgcaactatt ttctgttggg cagtgcatga gacgctattt
    28081 gacctggctg gtcagtggat gaaacaacag ataaagaaaa tgcgggcaat tcgcccgcat
    28141 gtcctgattt gtacacactc attttccgac tgacagttta catagcccaa acgctatcaa
    28201 atctgacagc ctgctatgag cgaaaagcgt taaatctcac gatatctgct accgggaaaa
    28261 atttcgatta tcttcgatgg gaactttgat aatgtagtaa atgccatttt cagcgatcac
    28321 ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac ttcactgaaa taaggttgct
    28381 agtatttttg gagcaaccat taatgttttt tatttaagga atgtaaatga catacgcact
    28441 gattgataat tcaactctaa ctgcagtcca aagaatcgag gggaaagtaa aaacaagatc
    28501 gaaggactca gtagatacag acataatagc atttgagaac tatatacagt ctattttgtt
    28561 ctatgatcac gttatcgcgg ttgatgatta tattccagag taccgtggga gtaggatttc
    28621 aagtttcaga gagatattat ttcttgataa agcacaatat gggttggatg aaattgaaag
    28681 tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt cgtggtggaa catttgttaa
    28741 tgacgatttt aaaaaactaa ttgaacttct tcagacacat atcatatgta catgggacat
    28801 tagttcaagt gtttatcatc taacactgaa gaacttatct gatggaggta aggagtttga
    28861 aaaatatggt aacattgctg ctgccatttt ttcagaactg cgtgatgctg ctgagtctgg
    28921 taatagaaca aatggcgatg ttgaacttct cgatcgattt ggcaacccta ttcgcaaggg
    28981 gtacaaagtt cccggagcaa aatgggggga tggcacatct gctggtgaag catcagatgc
    29041 aatcaaagca ttcgtggctt cgcttgtatg gctggctaat cgggcagtat tttattctct
    29101 cacagctaag taccttcaag cagatacttt tttatatcca ttacgtcagg cgtatcaaca
    29161 gacatattta agccaaaact gtcagtatgg ttttgactat gctaaacata ttgtagaaaa
    29221 cttttccact tcgttaagtc atgatctcat tgatattcat tctggtggta aagctgtagc
    29281 aacagcaact tcgcttcctt tattttcggc atggctcgca aaagaaactg gtgatccaag
    29341 ttgtattgtt gaagctgctt ataacataag ggacaacaac gaatttgtaa gggcccgtga
    29401 gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa ttgcctcaag ctaataagtc
    29461 agtaagagca ttgcttactg aagtatcgaa agaatcagat tatattcgaa ctaagtatgg
    29521 gctcaagact agacaaggtg tacctgtaac taagcttgta catgtataca atactgttgc
    29581 ggctattaat tcccttccga aactaccgac ctttaatttt aaagttaaag ttcctgattt
    29641 cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc tatcgaaatt tgactaatga
    29701 tctttctact gtgtggtcgt taggagaagc tcgagatatt cttggaagta aagttgtgat
    29761 agataatcat gcaagaattt acagtccgaa gcatgaagaa ccgcgtttca aaaactctca
    29821 ttccccatat aagagcccga tgtagcttaa gaaaaagaca cgcccacctt gtgcgtctac
    29881 ttctggcaca gagcggactc tcagattagg ctttactctg tgccatagat atgtaagctc
    29941 acaccagagc tcatacaact tattgcggca tttccggcca ttcaggattt gcaggatcca
    30001 cacgactgac cagaacactg tagcgttccc atgcctccag tcgactgcgc tcctcatctg
    30061 ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat cacggattca gcatctgcaa
    30121 gaagtctggc tttccgattt tctgcctgct gctgcaattc ctctgccgta taaattcgtt
    30181 taatcactgt acagtcctta aacatccagt tccctgaaat gtccgcccgt cggttagcag
    30241 taatatccgg cacttcaaca acacttaatc catccggtct gatagctgtc acatcctttt
    30301 ccacatagcg gataatatta tctttgtcgt acgctatttt tatcgtgtca tcagcaaaat
    30361 acttttgttc ttcgtaccag ttcttaccat cttctgaaaa aaaccagaca acatcaaagt
    30421 cctttgtcaa ttgatattgt tcaaccgttt ttggattacc tgccgttata tttatcaaat
    30481 gctgcataaa ttatacctgt gccacgttat accaagtccc gttaatgtat ttctgcaccg
    30541 gtctgtaata tacgccacca atgttatcgg cagagtttga gccggtatcc tgaacaataa
    30601 tgccggaata tacacacccg gacggtgcct gatgtgtcca tgtcatacca ttgttcgcag
    30661 gtttgtatgt ggcagcacca ccaagccgga tatcccggac atagcgtgaa tcaaagttgc
    30721 catagttaga tggtgatacc tgcccgttaa cagcaaaagt gatgctgtta tctgtatttc
    30781 tctgactgta aaaatgccag cctgcatcat cacctaattc agccaccaca ggacgacttg
    30841 agtttcccca caaattgaat gcggcttcct tcgtggatgt attgctgctg ctgaccgtga
    30901 actttttccc gctaccggca cgtactttgg tacttgagac aatatcacct gtaacactca
    30961 ggccatgccc cattgacact ccgccattgg cgttattgat agtcagcggt cttaagccgt
    31021 tccatgtccc aaatttatcg ccagaggccg tcagcattaa atatgtgctg ccgccatcat
    31081 tcctgataaa gaatccataa ttgccataag caatgcgcag accattagca ctgagtgatg
    31141 taatttcacc tcttgaacgg agaccataag cggagctgag tgataattct tcctgagcgt
    31201 cattgtttcc tgtcgcccag cgaattaccc cgccctgaac tgtttcatgc cagatagtat
    31261 ctccttctcc accacgaaac tttctgagat attttttgcc gcctctggtg cctgaacata
    31321 aggccgtaga catataggca ttctggcttc cgccatcctg attaatcgtt ccggtcattg
    31381 agtcgccctg acgattccag tcacgacgcc agccggggga gtagccgtcc ccatgattta
    31441 tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga tgttgttggc gtggtcactc
    31501 ggatagtgat tgcagattta gttcccatga cctcgacgac acaaccagcc aggtggatat
    31561 caccacatcc ggtatccgta atgattttgt tatttgcata tgaccaggag cctttgcaca
    31621 tccagtacgg atgattaaat gcaccacggg aatccagcca ttcaataaac tgagcggttg
    31681 tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc acgacaggca ccgatatttt
    31741 tcgtgaaggt atcctttccc ggaatatccg caccgttctg atctttctga agacgttttt
    31801 cagcattgtc ataggcagac ttcactgctt ttggcgttgc ggccagcgtt tcagaatcgc
    31861 tgttgatggc actacttagc tggacaagac cttttcgcgc tgtggtggca tcctgtgcag
    31921 tgtatttccc gttagcaagg tcatacgctg tcttaaccgc ctttggtgtt gccgcaagcg
    31981 tttcagaatc gctgttggtg gcgctactga gctggacaag accttttcgc gctgtagtgg
    32041 cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc tgtcttaacc gcctttggcg
    32101 ttgccgcaag cgtttcagac gtgctgttgg tggcactact gagctggaca agaccttttc
    32161 gagctgtggt agcgtcctgt gcggtatatt tcccgttagc aaggtcatag gcggccttta
    32221 ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt ggtcgcactg cttaactgag
    32281 taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg tgactgttcg tgctctgcaa
    32341 ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt ggtgtcaatg gtcagctcca
    32401 ccgaggccac actgctgacg atgatgacca tgcgacaggt ctgcgaacgc cctgagcctt
    32461 cggcaagagc tggcttataa ctttcggcca tgttcgccac ggcaattaac gttcccgcat
    32521 catcgtacag gccaagctca cgcatccaga aaccgcccac ctccggcgga ataaccagct
    32581 ctgcgataat ataattactg tttcgtttgt cctggctgat tttgttcagc gtatgtcgcc
    32641 agacttcgtg gataagcccg gtctgtccgg catccgggac aggcaattta ccaccgccat
    32701 ccccgacggc catcgtggta atgttgacct tccgccctcc cggtgcggtt gccgctgcca
    32761 gctttgctgc accggcagtg gtgataacgg ttctgaattt tgtgctcatt attcctcact
    32821 tatccggggt aaaccgtaat tacatcgccg tcgtaagcca caccaccggc gaacaggtag
    32881 ccgggaatgt cccgggtaat gttcaggcca ataagatgac ggcttgcagg tttggcatca
    32941 gcaataagcc gttccatttc ctgatacatt gcctctgtga tgccgctttc cagtacacca
    33001 atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc accactccgt cacgttgatg
    33061 agatagccga gcggctccac cacacgccgg attgcgccga tagtgccttt atgacagtgg
    33121 atgaaatagg catcgcggat aacggcgcgt tttgtcgctt ccggccactt ttcatcccac
    33181 ctgtcgaccg aaaacgccca cgccagccac ggcagcagat ttgccggaca ggtatccggg
    33241 ttccacagtt cacgaatcct gaccggcgtt ttttcaattt ccgcacaggc ttttgcggcg
    33301 gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg aatcactcat ccgagcctcc
    33361 ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta ctgttgagca cgatgtcggc
    33421 cagcggtgca gtcagttcga cacgctgcac gccttccaca tgcaaagcgg cataaatggc
    33481 agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg atgtacgctt ccagtttttt
    33541 cacggcagca gcgcgtatgg gttcgctttc gggaccaggg taaaggtaaa gcgtggcgtt
    33601 tatctggtat tcaacgatgg cggcagactg cacggtcacg cggtcggcca ccggcctgac
    33661 gtcctcacca ttaagggcgt tacgcaccac cgccagcagg tcttcggatg cgacaccgtt
    33721 attttcacgt gacagcacag agatggtgac gcaggccgga gacggactgg ttacagaaat
    33781 atccgcgaca cgcccgtcgg cactgcgacc atgatactga taggccccca ccgacccggc
    33841 gacgcttaaa ccttcaaacg cctgctgaat acgcagacga taatccgtgt cagattccat
    33901 cactgccggt gtcggcggga gggtcgaatc atctgccggg gtgataatca ggcgcgtggt
    33961 gttgtaattg gcaccaatca catcaaggtc attaccggcg gcacaggcca gcattaccgc
    34021 ccgtgcggcc tcattcacac gctgacgcca gataagctca cgataagcat tttcctccag
    34081 cagtttgacg agaggctcgg attccagcgt cagggtacgg gcgaccgcct cctgctggtc
    34141 ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca agaatggttt caaagtccag
    34201 ctcctcgacc acatccggtg cgggtagctg gttcaggtcg ataatcggca tggtttcaac
    34261 tcacagggat ggttaacgaa agtggctggc cggtgtcgtt gtgctggccg gttaacgtga
    34321 ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga tgacagggtg acgcgggctt
    34381 cccatttcag cacggccatg taacaggcga ccttaatctg caactcaagc gccggggtct
    34441 gcggctggtc aatcattgac gccagcaacg agccgtaatc acgacgcatc acccgtgagc
    34501 cgaccggcgt gcgcaggata tcgccgatac tctggctgat atgctcaagg tcagagacag
    34561 tcaggccatc actgcgattc attccgagat aacgcgctgt catagaggac tcccggttgt
    34621 gccgccgctg tcgccggggt gtttatgggt atgcagtacc ttcccgtttg atgagagttc
    34681 accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc ttctgcactt ccagcgtgcc
    34741 agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc agagtgacgc gggttgatgc
    34801 tttcaccgtg accaccggca ccgtggcagt aacagaatca gaagccgtca cgctggccgt
    34861 tttaattccg cttaccgtga gtgcactggt ttcaggttca tactcaatca ccgccccgtc
    34921 agggaaacgg atatgcaggg catccgccga cgcagacggc gcggggttat caccggaata
    34981 aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct acggccagaa tcagcacctg
    35041 ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg gcgcgatggg tcagccactg
    35101 aagccagtcg gtgcacatgc cgccggtctg cacacggcag cgaccggcat taaggtcggt
    35161 ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg cgcgcgagtt cctgaatatt
    35221 tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat accggcggca atgtgacgat
    35281 gaggtgtcag gaatggcaca actaacggtc gaggtgagcc aggataatct cttcaatcat
    35341 ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc ggataatgaa ttttcttacc
    35401 gtctttccgg gtttcttccg acagaccgaa ctgatgcaca ctggctattt tcggtgactt
    35461 cccgccgtaa aactccattg atgcctgttc agggctggcg cggatatgca aaaaacgact
    35521 ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg gtcttttttc tggcgctctg
    35581 ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc tgtgccatca cccgacgctg
    35641 ctgactctga cgcagacgtt tcgccagttc ggcgctcagt cgccgacgcc ctgacggtga
    35701 cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc ttaaactcat tcatcccact
    35761 tactcaccag ttcgccattg atataaagct ccatcgggcg ggtgaccggc tccggcggcg
    35821 gaggttccgg gatattcttc acatgcagcg cgccgtccac ctcactgacc agcgtgcgct
    35881 cggtcagcat caggctgatg ctgatatcaa agctgctgtc attgttgatg tctgcataaa
    35941 acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat gtcgggctga ttttcccgca
    36001 gccacgccag caccggcacg atgagcaggt caaaatcacc ggtaaagtcg gtcacaatca
    36061 cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc cagtgtggag gcaatactcc
    36121 cgttatccac gaatatccgc agcatctcgg ggcttgtttt cagcaccgtg acggcatcag
    36181 tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt cgtcctgaca gtgtttaatc
    36241 atttttacct ggctggcaca gcgtgccagc gcgttctcaa gctgccggat atcagcactt
    36301 aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc aaaggctcac tttcgggcag
    36361 gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc tggtgcaacc ggcgcacagc
    36421 atcaggcagg tcagcaccgt accagcggcg aaaatcttcg ttttcattaa gtaacctcgt
    36481 gatggttttc tcgcgctgag cttcacgctt cgcggcgttc tccagttcct gacgcagtgc
    36541 cacctgcgcc agctcgtttt tgtctgccct ggtgagggca acatgaagct ggtttttcag
    36601 catggtgatg gtcgtctgct gttcactggc gacgttgttc gccctgtcca gcaaggcgcg
    36661 caggctggca tttttgtgtt tcaccagaaa cagaccggcc accgccagcg ataacaacac
    36721 aaccagcaca atcatcagtt ttgacatggt tcccgcccct caagacgctg acggcaggcc
    36781 gtacgtatca gccggaaaaa cagcgatgcc acgagataaa tcagtgcggt aaaaatccac
    36841 ccggcagcga ccagcgagat aaacgtcgcc accatcacca tcagagccgc tgcccgtctg
    36901 cgccacggca ccggctgcaa aaacagcgac gtgacaatct tcacggccag cgattccggc
    36961 ggcagctccc gcccgtagcg ttccagcaca tactccgcgg catacacgcc gacaccaccg
    37021 gcaatcacac agataaccgt cgccagaatc gcccatgcag cgacaaaact gacggccacg
    37081 ctctgcgggt aaatcaggga cagtgccagc atcagcgcca gcgacacgtt cagcatcagt
    37141 gaaagggata atttcttcat ggtgtttact ccgtttaagc cggtacgccg ccagcggtgc
    37201 gccagacggt gaccagtttt tccagtgaat gctcacgctg accgtaaccg gcacccggca
    37261 gggacgccca gatattgcga cagcgtgaaa tggcgcgctc aatgcgtccc gcccggatgt
    37321 catccagtgc accgcgttcg cggatcaact gaatggcgag cctgtcctgt gacaacggac
    37381 tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca gaacaggtaa agctgctgat
    37441 agcgaccgga ggccgtggat ttttcaccgc gacggttaaa caccttcgcc ggtcggccat
    37501 gtgcgaacgg gtggtcactg tagtcggtga agatttccgg cttcccgtcc agtccggtga
    37561 ctatcacgtc atagccccgg tttttcgtca gcggatgatt cgccgtccct tcggacacgg
    37621 ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct aattaccggc attacggttt
    37681 ccccctgccc ttaaagcggc gctgaatggc aatctcaatc acctgataac cggcgatacc
    37741 cagcatggag ccgatgccgc acaccgcagg cagtgacagg tcaggaaact gcaccagaac
    37801 aacaccggca accatcgaga caaaaccacc gagcaacatg cgcccgataa acagacgcgg
    37861 agtgatgggt tcaccaccgg caagcacctt gccgacaaca atcagcaccc caatcatgaa
    37921 aagcgacagg acgctttttt cttctgctgt catgcgttac tcccacagat tgacagtttc
    37981 agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg gtgccgtgtg gcagcaccgc
    38041 ccccagttca gccagtcccg gatttgcggc gagcacggcc tcgaccacgc cctcagtgcg
    38101 cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc tgtagcgcaa aggtcttcat
    38161 cagatttgac tcacgatgca gcgcggcttg tcctggatac gcgccaccgc ccagcgcata
    38221 tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg ccttcttgtc gcccttggca
    38281 ctggcatcca cgccgcggta acgctcataa agcgacgcgg tcgccatcgc acacacagcg
    38341 cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt cgtccgccgg gacgtcagcc
    38401 agacgcgaaa aaccggcagc aattttctgt tcgcggtact cgtacagctc cgcattcgtc
    38461 tccgccatgc ctgacttgat ggcctcacgc agacgggcgg gggcgacggt ctgctcaagg
    38521 cgcatacgtt cccggacgcg cttcgggtca atatcgggaa aaaagaacgt gtttttaatc
    38581 accggctcgt cgcctgccgg ttgcgggata accaccgtac cctcaccgga cacgggagcc
    38641 tcctttcgcg gaataatcag cgtcatcatg actacctctg aaaagtcggg cggtggacgc
    38701 cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg ccgccctggc gcggggcgca
    38761 ttcggttgtt aactggcttt ctttttcggg cgtccacgtt ttgccggtgt cacgctccgg
    38821 gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct ccggcttcgg tttcagctcc
    38881 cgctccagtc gttcaatctc ttttttgacg cctgcctgac agtcgagctg tgtcgcacgt
    38941 tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac gcagaaacag accggtgatt
    39001 ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg tggcggtcag ttcgagggtg
    39061 tccgtcagca ggcggggatc cacagactca ccggcagcgt gagcgcgcat ggccgcaagc
    39121 gccacctcct cggtgaacat gtacggcggg gtgcggcggt gtttacccgg catggtcaga
    39181 ccgtacttca gggcataacg ggcaatctcc agcgcaccgg caatatcgcc ggtatccaga
    39241 cgccacagca tgaccgtcat cagaatgtca tcctgtgcac ctttgccctg ctccagcacg
    39301 ccgttcaccc acggcaacca gaacggcagc agttcgcgtt ttttcgcggc cttcagctct
    39361 tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca gcttgaccag catctgctca
    39421 tagacagttg catgtcgcag cggggcggct tcccgctgcg cggtcatcgc tgccgagacc
    39481 cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg ctcccggctc tgcggtcgct
    39541 ttagccggtg tggagaaatc accgacctta attttttcca ccagacaacc ggcggcgtag
    39601 tcctccacca cgtaatcaat gttcattgac tcgtagttct ccacgcggtc gagtttcggg
    39661 ttttccacaa tcacgcggcg atggctgtca tccatgtagt agatggacag gttttccagc
    39721 ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc gtaccgccgg caggttaccg
    39781 atgcgtttct ggctgatgat gacgtcagcg gccagcatct cgctgttatc ctgctccttg
    39841 ttgacgatag ggaaatactt gtccgccagt agctgacgcc ccacaatcac cacaaggtca
    39901 gggtcttcct gataccaagg ttcaatcagg ttgttggtcg catccatcac cagtgcatcg
    39961 aggcttacat aatcaccgcc cttacccacg cggataacct cagaggtggt gcggccttcc
    40021 tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat tcggtatttc tgcagccagc
    40081 cgaccgccac atcctgcagc atcggattac tgttgcggtc agaggtttcg gcacgcttca
    40141 cgccgttaaa accggccatg atgaaatcaa gggactggcg tttgataatg gcgttacgga
    40201 tacggagctg gaaatcctga taacgcgccc acaggtcaag cgttttgtag cggatataaa
    40261 aatcgaagtt aacctggtcg cattcgtact tgtttgacgc cagcttcgag aagtccttcg
    40321 gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc gatggagccg gtgacaccga
    40381 tgccaatttt ttcccctttc atttcactga ccggcacaat gttgatgcgg gtcagaaagt
    40441 cagaggactc ctgcatggtg ttcatcaggg tctgggtgac cgacggttca acggtgaatt
    40501 ttttcgacac atcaccggcg tcgatgccgt tcagctcggc aacacgggac aggtaagcat
    40561 taaatttaaa gcgggtttcc tggcgcatag tttttcctga aattaagggt taatcgtgaa
    40621 ggttttcccg gactgactga cgccggtcag cagttcgtca tcagggcgtc accgccacca
    40681 ccggtggcct tgctgcggcg ctgctgggtc agactttcgg tgtggtcgag actgtttttc
    40741 aggcgggtga atgcctggct ggtttcatcc gccctgtcag tcacctcctg cttaagtgca
    40801 gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg cgctcagttt ttcctgcaca
    40861 tgttcagcaa cagcggtcac cgcttcatgc acgtcattca gacgggcatc atcgctggcc
    40921 tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg cggtgaacac ggtttcaggc
    40981 aggtcttcaa attccagctc aacaggcgtt gccactgaaa tcaggttttc agggcttaat
    41041 ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga attccaggta ttccgtgccg
    41101 aggcttgccg ggtcatcggt gacggccaga cccaccagat aacatttgcc ggtgttggca
    41161 aagttcggct gaatttccat tgaggtgtag accttctgcg cggccttgtt catcgcgata
    41221 aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt tgcctttcag cgccgaatca
    41281 tcgtcaatct tttcggcctt cagttcggcc acatcgccat aacgcttaaa aataccgtca
    41341 ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac catagacacg cgggtcaaag
    41401 gtttcggcca tttcctgaat atcctgcgca ctgatgacac gcccgtcaca ggtgtcaccc
    41461 tcaacgccga tacgaaagaa ttttgagact ttttttgcca ttgtcaggag tcctgaatag
    41521 tgattagagg agtcacatgt cggcatcagt ttcccgacga tgcgcatcct ccgccatcag
    41581 tcccggatgg tttatcactg acacaacagc acattagcga atcgcggggc gcgactcagt
    41641 agccttgccg tgtattcatc acggcgaggt attcatgacc atcaccacag acaccactct
    41701 tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg caggggtttt ccgtgccgca
    41761 gattgccgcc atgttgcaga tgaaacgccc gacggtgcag agctggaaac agcgcgacgg
    41821 ctgggacagt gttgccccca tcagccgtgt cgaaatgagt ctggaagcgc ggctgaccca
    41881 gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag gaaattgacc tgctcggacg
    41941 ccagattgaa cgactggcac gggtaaaccg ctacagccag accggcaacg aggcagacct
    42001 taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc aaaccgaaaa agaatttttt
    42061 cagtgacgag gccatcgaaa agctggagca gattttcttt gagcagtctt tcgactatca
    42121 gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc gatatcctga aatcccgcca
    42181 gattggcgcg acgttttatt tttcccgcga ggcgctgctg cgcgccctga aaaccggcca
    42241 taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat gtgttccgtg aatacatcat
    42301 cgcctttgct cgtctggttg acgttgacct gaccggtgac ccgattgtcc tgggcaataa
    42361 cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc gcacagagcc ataacggcga
    42421 cctgtacgtc gacgagattt tctggatccc gaattttcag gtactgcgta aggtggcatc
    42481 aggtatggcc tcacagagtc acctgcgctc gacctatttc tccaccccgt ccacgctggc
    42541 gcacgacgcc tacccgttct ggtcgggtga actgttcaac cggggacgcg ccagcgccgc
    42601 cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc ggtggtcttc tctgtgcgga
    42661 cggccagtgg cggcagattg tcaccattga ggacgccctg aaaggcggct gcacattgtt
    42721 cgacattgag cagctcaaac gcgaaaacag cgccgacgat tttaaaaacc tgttcatgtg
    42781 tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag gagctgcaac gctgcatggt
    42841 cgacacgctg gaagaatggg aagactacgc gccgtttgcc gcaaatccgt tcggctcccg
    42901 cccggtatgg attggttacg acccgtcaca ccgtggcgac agcgccggat gcgtggtgct
    42961 ggcaccgccg gtggtggccg gtggcaaatt cagaatactt gagcgtcacc agtggaaagg
    43021 catggacttt gccacccagg cggagtccat ccgcaaactc accgaaaaat acaacgtcga
    43081 atacatcggt attgatgcca ccggcctcgg tgtcggcgtg ttccagctcg tgcgctcgtt
    43141 ctatcccgcc gcgcgcgata tccgctacac gccggaaatg aaaaccgcaa tggtgctcaa
    43201 ggccaaagac gtcatccgcc gtggctgtct ggaatatgac gtcagcgcca ccgacatcac
    43261 cagctcgttc atggctatcc gcaagaccat gaccagcagc ggacgcagtg ccacctatga
    43321 ggccagccgc agcgaggaag ccagccacgc cgacctcgcc tgggcaacca tgcacgccct
    43381 gttaaatgag ccactcaccg ccggtatcag cactccgctg acatccacca ttctggagtt
    43441 ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg cggcaaaaac aatcaccgcc
    43501 agcgccccga aaatggaagc attcaccttt ggtgagccgg tgccggtact cgaccgccgt
    43561 gacattctgg attacgtcga gtgcatcagt aacggcagat ggtatgagcc gccggtcagc
    43621 tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc acagctcacc gatttacgtc
    43681 aaacgtaata ttctggcttc aacgtttatc ccgcacccgt ggctttccca gcaggatttc
    43741 agccgttttg tgctggattt tctggtgttc ggtaatgcgt ttctggaaaa gcgttacagc
    43801 accaccggta aggtcatcag actggaaacc tcaccggcca aatatacccg tcgtggcgtg
    43861 gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc cgacagcctt cgcgcccggc
    43921 tccgtgtttc acctgctgga gcctgatatt aatcaggagc tgtacggcct gccggaatat
    43981 ctcagcgccc ttaactctgc atggctgaat gagtcggcca cgctgttccg ccgcaagtat
    44041 tacgaaaacg gcgcacatgc cggatacatc atgtacgtca ccgatgccgt gcaggatcgc
    44101 aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga aaggccgcaa taactttaaa
    44161 aatctgtttc tctatgcccc acaggggaaa gccgacggca ttaaaattat cccgctcagt
    44221 gaagtggcaa cgaaggacga tttttttaat atcaaaaaag ccagcgccgc tgacctgctg
    44281 gacgcgcacc gcatcccctt tcagttgatg gggggcaagc cggagaacgt cgggtcgctg
    44341 ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg agcttatccc gttacaggac
    44401 aggatccgcg agataaacgg ctggctcggt caggaggtca tccgctttaa aaactactca
    44461 ctggacactg acaacggctg aacatcgccg cctgcgggcg gcttttttac accccgccat
    44521 cacgccctca cacgctcatc accgcacaaa aaatcccgca gacacaccaa cgcccaacgg
    44581 gcagactaag cgccgtcacg acgcgctgag acgctggaaa ataaaatcag caccaccgcc
    44641 agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg gggcggtttt aatgcagttg
    44701 catgaacact ccaagagcac gccagcgcta gtgataacta acaataaaag cgacttctga
    44761 cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc tatgcggtat gctatccgct
    44821 tcaattttta acacttgtta agggaactag acatggacat tattgattta aaatctcaac
    44881 tagaaaatga agataatgcc aagaaaagag cagagttgct tgctcagcta gaggtgataa
    44941 agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga tgtggatttt gagaccaaag
    45001 aatttaccgt tgagttatta gttaataagt atcataacgg attagaggat gatactaacg
    45061 aactctttgt tcctgattat caaagggatt ttgtttggag tgaaaaaaga caatcaaaat
    45121 tgatagagtc
//
LOCUS       DAZPJP010000011        44313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPJP010000011 DAZPJP010000000
VERSION     DAZPJP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.011"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHU9J_000564"
     CDS             complement(<1..559)
                     /locus_tag="ACHU9J_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1904203.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHU9J_000565"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHU9J_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1904204.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHU9J_000566"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHU9J_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1904205.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHU9J_000567"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHU9J_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1904206.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHU9J_000568"
     CDS             complement(4001..6322)
                     /locus_tag="ACHU9J_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1904207.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHU9J_000569"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHU9J_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1904208.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHU9J_000570"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHU9J_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1904209.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHU9J_000571"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHU9J_000571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1904210.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHU9J_000572"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHU9J_000572"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1904211.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHU9J_000573"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHU9J_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHU9J_000574"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHU9J_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1904212.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHU9J_000575"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHU9J_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1904213.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHU9J_000576"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHU9J_000576"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1904214.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHU9J_000577"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHU9J_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1904215.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHU9J_000578"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHU9J_000578"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1904216.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHU9J_000579"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHU9J_000579"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1904217.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHU9J_000580"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHU9J_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1904218.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHU9J_000581"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHU9J_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1904219.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHU9J_000582"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHU9J_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1904220.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHU9J_000583"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHU9J_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1904221.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHU9J_000584"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHU9J_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1904222.1"
                     /translation="MKQWIAALLLMLIPAVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHU9J_000585"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHU9J_000585"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1904223.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHU9J_000586"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHU9J_000586"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1904224.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHU9J_000587"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHU9J_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1904225.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHU9J_000588"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHU9J_000588"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1904226.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHU9J_000589"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHU9J_000589"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1904227.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHU9J_000590"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHU9J_000590"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1904228.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHU9J_000591"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHU9J_000591"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1904229.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHU9J_000592"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHU9J_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1904230.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHU9J_000593"
     CDS             32202..32564
                     /locus_tag="ACHU9J_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1904231.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHU9J_000594"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHU9J_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHU9J_000595"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHU9J_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHU9J_000596"
     CDS             35034..35912
                     /locus_tag="ACHU9J_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1904232.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHU9J_000597"
     CDS             35923..36276
                     /locus_tag="ACHU9J_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1904233.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHU9J_000598"
     CDS             36288..37592
                     /locus_tag="ACHU9J_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1904234.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHU9J_000599"
     CDS             37604..38689
                     /locus_tag="ACHU9J_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1904235.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHU9J_000600"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHU9J_000600"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1904236.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHU9J_000601"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHU9J_000601"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1904237.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALCLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHU9J_000602"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHU9J_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHU9J_000603"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHU9J_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1904238.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHU9J_000604"
     CDS             complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHU9J_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1904239.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44010..44177)
                     /locus_tag="ACHU9J_000605"
     CDS             complement(44010..44177)
                     /locus_tag="ACHU9J_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1904240.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        10351 a        11226 c        12120 g        10616 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccgccgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatag ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaggc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacacagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggt gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt cccatagcga tacctttttc ttgtccaata
    43741 cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat gatgaatata
    43801 acaacatatg atgttatgta atgtggagga agatcacaaa atttcgcaca ggatcgcgct
    43861 gtggctaatg gatgtagtta tcaaattgaa tttaaagtga aaatattttt acgggcgggg
    43921 gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc gggcaattaa
    43981 accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca accgccaatg
    44041 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    44101 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac cagtgcaact
    44161 ttaataaatt tgttcattta cattccctat gtattatata ttaattaata ttcattgatt
    44221 atatgaatat aagcagactg ctcgttaaga acgatacagg aatatttaat tggcgtcagt
    44281 gagcgaatga cttaattttg taagtcgctg taa
//
LOCUS       DAZPJP010000012        43892 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPJP010000012 DAZPJP010000000
VERSION     DAZPJP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.012"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHU9J_000606"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHU9J_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1904241.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="ACHU9J_000607"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="ACHU9J_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1904242.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3005..3086)
                     /locus_tag="ACHU9J_000608"
                     /pseudo
     CDS             complement(3005..3086)
                     /locus_tag="ACHU9J_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHU9J_000609"
     CDS             complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHU9J_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1904243.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHU9J_000610"
     CDS             complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHU9J_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1904244.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHU9J_000611"
     CDS             complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHU9J_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1904245.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNSDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4175..4669)
                     /locus_tag="ACHU9J_000612"
     CDS             complement(4175..4669)
                     /locus_tag="ACHU9J_000612"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1904246.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPVLEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHU9J_000613"
     CDS             complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHU9J_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1904247.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHU9J_000614"
     CDS             complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHU9J_000614"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1904248.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHU9J_000615"
     CDS             complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHU9J_000615"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1904249.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHU9J_000616"
     CDS             complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHU9J_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1904250.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHU9J_000617"
     CDS             complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHU9J_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1904251.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHU9J_000618"
     CDS             complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHU9J_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1904252.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11283..12323
                     /gene="gpr"
                     /locus_tag="ACHU9J_000619"
     CDS             11283..12323
                     /gene="gpr"
                     /locus_tag="ACHU9J_000619"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1904253.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12363..12857)
                     /locus_tag="ACHU9J_000620"
     CDS             complement(12363..12857)
                     /locus_tag="ACHU9J_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1904254.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13048..13932
                     /gene="yghA"
                     /locus_tag="ACHU9J_000621"
     CDS             13048..13932
                     /gene="yghA"
                     /locus_tag="ACHU9J_000621"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1904255.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14092..14517)
                     /gene="exbD"
                     /locus_tag="ACHU9J_000622"
     CDS             complement(14092..14517)
                     /gene="exbD"
                     /locus_tag="ACHU9J_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1904256.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14524..15258)
                     /gene="exbB"
                     /locus_tag="ACHU9J_000623"
     CDS             complement(14524..15258)
                     /gene="exbB"
                     /locus_tag="ACHU9J_000623"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1904257.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15510..16697
                     /gene="metC"
                     /locus_tag="ACHU9J_000624"
     CDS             15510..16697
                     /gene="metC"
                     /locus_tag="ACHU9J_000624"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1904258.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16837..17496
                     /gene="yghB"
                     /locus_tag="ACHU9J_000625"
     CDS             16837..17496
                     /gene="yghB"
                     /locus_tag="ACHU9J_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1904259.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17536..18435)
                     /gene="yqhC"
                     /locus_tag="ACHU9J_000626"
     CDS             complement(17536..18435)
                     /gene="yqhC"
                     /locus_tag="ACHU9J_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1904260.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18629..19792
                     /gene="yqhD"
                     /locus_tag="ACHU9J_000627"
     CDS             18629..19792
                     /gene="yqhD"
                     /locus_tag="ACHU9J_000627"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1904261.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19897..20724
                     /gene="dkgA"
                     /locus_tag="ACHU9J_000628"
     CDS             19897..20724
                     /gene="dkgA"
                     /locus_tag="ACHU9J_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1904262.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20924..21850
                     /locus_tag="ACHU9J_000629"
     CDS             20924..21850
                     /locus_tag="ACHU9J_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1904263.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21901..22158
                     /gene="yqhH"
                     /locus_tag="ACHU9J_000630"
     CDS             21901..22158
                     /gene="yqhH"
                     /locus_tag="ACHU9J_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1904264.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22201..24420)
                     /locus_tag="ACHU9J_000631"
     CDS             complement(22201..24420)
                     /locus_tag="ACHU9J_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1904265.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24531..25943)
                     /gene="ftsP"
                     /locus_tag="ACHU9J_000632"
     CDS             complement(24531..25943)
                     /gene="ftsP"
                     /locus_tag="ACHU9J_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1904266.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26018..26755)
                     /gene="plsC"
                     /locus_tag="ACHU9J_000633"
                     /gene_synonym="parF"
     CDS             complement(26018..26755)
                     /gene="plsC"
                     /locus_tag="ACHU9J_000633"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1904267.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26988..29246)
                     /gene="parC"
                     /locus_tag="ACHU9J_000634"
     CDS             complement(26988..29246)
                     /gene="parC"
                     /locus_tag="ACHU9J_000634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1904268.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29792..30274)
                     /locus_tag="ACHU9J_000635"
     CDS             complement(29792..30274)
                     /locus_tag="ACHU9J_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1904269.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30327..30719)
                     /gene="ygiW"
                     /locus_tag="ACHU9J_000636"
     CDS             complement(30327..30719)
                     /gene="ygiW"
                     /locus_tag="ACHU9J_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1904270.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30871..31530
                     /gene="qseB"
                     /locus_tag="ACHU9J_000637"
     CDS             30871..31530
                     /gene="qseB"
                     /locus_tag="ACHU9J_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1904271.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31527..32876
                     /gene="qseC"
                     /locus_tag="ACHU9J_000638"
     CDS             31527..32876
                     /gene="qseC"
                     /locus_tag="ACHU9J_000638"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1904272.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32986..33567
                     /gene="mdaB"
                     /locus_tag="ACHU9J_000639"
     CDS             32986..33567
                     /gene="mdaB"
                     /locus_tag="ACHU9J_000639"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1904273.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33598..33912
                     /locus_tag="ACHU9J_000640"
     CDS             33598..33912
                     /locus_tag="ACHU9J_000640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1904274.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33960..35852)
                     /gene="parE"
                     /locus_tag="ACHU9J_000641"
     CDS             complement(33960..35852)
                     /gene="parE"
                     /locus_tag="ACHU9J_000641"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1904275.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35881..36462)
                     /gene="yqiA"
                     /locus_tag="ACHU9J_000642"
     CDS             complement(35881..36462)
                     /gene="yqiA"
                     /locus_tag="ACHU9J_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1904276.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36462..37289)
                     /gene="cpdA"
                     /locus_tag="ACHU9J_000643"
     CDS             complement(36462..37289)
                     /gene="cpdA"
                     /locus_tag="ACHU9J_000643"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1904277.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37314..37736)
                     /locus_tag="ACHU9J_000644"
     CDS             complement(37314..37736)
                     /locus_tag="ACHU9J_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1904278.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37737..38366)
                     /gene="nudF"
                     /locus_tag="ACHU9J_000645"
     CDS             complement(37737..38366)
                     /gene="nudF"
                     /locus_tag="ACHU9J_000645"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1904279.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38571..40052
                     /gene="tolC"
                     /locus_tag="ACHU9J_000646"
     CDS             38571..40052
                     /gene="tolC"
                     /locus_tag="ACHU9J_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1904280.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40200..40871
                     /locus_tag="ACHU9J_000647"
     CDS             40200..40871
                     /locus_tag="ACHU9J_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1904281.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40877..42037
                     /locus_tag="ACHU9J_000648"
     CDS             40877..42037
                     /locus_tag="ACHU9J_000648"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1904282.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42075..42863)
                     /gene="ygiD"
                     /locus_tag="ACHU9J_000649"
     CDS             complement(42075..42863)
                     /gene="ygiD"
                     /locus_tag="ACHU9J_000649"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1904283.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43006..43779
                     /gene="zupT"
                     /locus_tag="ACHU9J_000650"
     CDS             43006..43779
                     /gene="zupT"
                     /locus_tag="ACHU9J_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1904284.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
BASE COUNT        10752 a        11803 c        11084 g        10253 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
       61 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc ccacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac agcgaccggc
      301 aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac tagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccaa
      661 tccacgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca attcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtactcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
      961 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     1141 aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttcag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     2461 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatttg
     2521 gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta cgccatcaac
     2641 cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aatgtggtgt taggcggcgt gtatgatgcc gcagagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     3061 acgctgccgc gtcttatcag gcctacgaaa gctaatcatc acgcgctggt aatgtcgtaa
     3121 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3181 gcaaatccga cgtgtaccag cacccactgg cccagtagat cggcagggtt accttcacaa
     3241 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3301 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattat taactccggt
     3361 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
     3421 ccgtgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3481 cagcaccaag cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
     3541 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
     3601 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg
     3661 ataatttcaa cggcgctctg gcaaagagac aactcatgca ttttcgccac tcctgcgacc
     3721 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgaa ttcgtgactg gcagcgaaag
     3781 gatcattcgt gcgcagtcat ccgtcaggcg ttggccctct tcaatcgaca tgctgtgcga
     3841 aagcggcgac atcagcgaac aggagagata ttgcgaaaca ccgtccagtt cgccaacggt
     3901 aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg
     3961 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
     4021 ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttgaaggatg
     4081 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
     4141 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga
     4201 tggcttccac gcctgattcg cgcagcgcag ccagaacctg ctcaagcaca ggttcaatca
     4261 ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag cgattccggg atcacgccga
     4321 ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat acgtcggcca
     4381 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
     4441 ggatcatcat cgttcccggc gtgttctttt tcgacacaat ggcatccgca ataatcagat
     4501 gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct
     4561 caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acaccgatgg
     4621 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
     4681 cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg
     4741 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcag caaccggtgt
     4801 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
     4861 ccaggttgat ggaacaactg cctggtagtt actgataata ccgtctttaa taaccatcca
     4921 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
     4981 cggaatgttc ggtttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
     5041 actgtattga ttttgcagga tatcctgcaa ttcgcagcag tgaacggtac gaccaataat
     5101 acggcccagc gtggagtgca gctgcgccac ttccagcgtg ttgccagtca gtttctgata
     5161 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
     5221 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gtgatttcac
     5281 ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca
     5341 cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
     5401 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
     5461 aatgtagccg cccgggaaca ggaagctgcc gtttttactg tcggtcggga attccggcac
     5521 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcaat
     5581 aactgcggta tcaaccttat aaacctgttc aacaaagtcg ctcagcttgt cgatgaaaga
     5641 cttgatgtac atcaggcgct caaggttcag cacgcccaaa ccgtcgaggt tgatcgggtt
     5701 cgcgacacca cctaccgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
     5761 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag
     5821 gttcacttcc ggcggcagtt tcatcgccgg gtgacccccg tagccattgg cgaaaatacc
     5881 caactgaccg ctggcaacca gatctttgat cttgttctga actttagtga actcttccgg
     5941 actgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc
     6001 ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat
     6061 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
     6121 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
     6181 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
     6241 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
     6301 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
     6361 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
     6421 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
     6481 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
     6541 gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac
     6601 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag
     6661 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
     6721 gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat cgccagcaac
     6781 acactgatgg tgttggtcag cttgacgaac agactctttt catccggacc gttgccacgc
     6841 agacccgcct gcaccagcga accttcaaag atgacaatcg agaagcccat gatgaacgcc
     6901 gtcagcagcg agaacagcgg caacatttca tagctctgcc acaacggatg caccttgtag
     6961 cccgccgaga tcatcagtga ccccattgaa gactggtgca tggtcggcaa cagcgcgcca
     7021 agtgcaatga tgaagaacat aaccttgttc agacgcttga gagaaacctt ccagcccaga
     7081 cgttcaaaca gtgccggagc aaactccagt gccatcacgc cgatatagat ggtcatacag
     7141 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
     7201 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac
     7261 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
     7321 accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccaatcag caggtcaaac
     7381 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
     7441 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
     7501 ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat cgtctcctcc
     7561 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
     7621 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
     7681 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
     7741 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
     7801 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
     7861 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac cgaaaatcac
     7921 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
     7981 acctttctgg ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
     8041 cttcggcacg ttgtacggac aggcgaccat gcagtaacgg caaccggtgc acacgtcttt
     8101 gtcgtaatgg acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga
     8161 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc
     8221 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
     8281 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggaa
     8341 gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta
     8401 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
     8461 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
     8521 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
     8581 accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa accaatagcg
     8641 cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc
     8701 tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc ttcttcgtta
     8761 cagccatagc atgggtgacc aatcgctacc ggccacacgc cgccaacatc gcagaattgc
     8821 agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag
     8881 ccttcacggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
     8941 cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca
     9001 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc
     9061 gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac ttcttgcagg
     9121 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc agagcaggaa
     9181 ccgatagcga taatcgctgc tgcgccttct gccgctttgc ggatgtgatc cacaatcggc
     9241 tcaccagcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccacaacc
     9301 aacacatact gccctttgta cttctcaagt gcgttatgtt tgttctcttc gacctgatga
     9361 ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag cacgaggttt
     9421 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
     9481 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg
     9541 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
     9601 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
     9661 gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
     9721 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga
     9781 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
     9841 cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
     9901 aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg
     9961 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
    10021 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    10081 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgctata
    10141 tatcgccata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca
    10201 tgcttgttta agacgtaatc cagtaagaga taaacctatc aggagagata tttatcgaac
    10261 catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    10321 tcattatgga atccgtgatt aacccccgga tagatatacg cctcataaac cttattattg
    10381 gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc cagttccgcg
    10441 aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca
    10501 taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca
    10561 ccgccatagc aaaatccggt aatacccact ttgcctgccg cttgtggata gcgttgcata
    10621 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc
    10681 tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaagcc gtcaggtgcc
    10741 agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt
    10801 ccacgattct catgcaccac caccacggct ggcgttttgc cgctcatttt tgcgggtttc
    10861 accagataac cccgtacctc gccgtgacca tttggcgaag gatacatgat gtactcagca
    10921 acaatctccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa
    10981 tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
    11041 tcacgtttcg aaattttccc gtgagcgtaa tagtcgtagt aatccaacaa ctcttgtggg
    11101 aaatctttgg cggttaaacg cggcatcgtt gcactcctca gttggtgttt ttttaagcaa
    11161 agcataagca cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac
    11221 gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg
    11281 tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcaataccgc tattgcggaa
    11341 aaagcggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta
    11401 acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttgggc attacgcact
    11461 ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac tttggtcgcc
    11521 tgctgcggga ggattttgcc gcttatcgcg atgaactgat tatctctacc aaggctggct
    11581 acgatatgtg gcccggccct tacggctctg gcggttcacg taaatacctg ctcgccagcc
    11641 tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac tctcatcgcg
    11701 tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg
    11761 gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg
    11821 agttgctgca cgagtggaaa attccgcttt taattcatca accttcgtac aatttactga
    11881 accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgta
    11941 ttgcctttac tcctctggct cagggattgc tgaccggaaa atatctcaac ggcattccgc
    12001 aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg aaaatgctta
    12061 ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag cgtggacaat
    12121 caatggcgca aatggcgtta agctggttgc tgaaagatga tcgcgtgacg tcggtattga
    12181 ttggtgccag ccgcgcggag caactagagg agaacgtgca ggcgctgaat aatctgacat
    12241 ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggcgag ctgaatctgt
    12301 ggcaggcgtc gtccgataaa tgacctgtta ataacgggcg cgcgaaccgc gcccgaatca
    12361 gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc agaaagcaca
    12421 aacgtcagat ggataatgac gtaccacatc agtttgttat ccgggacatt tttcgcatcc
    12481 ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt
    12541 ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt ctcggagata
    12601 tccagctgtg agacgaaatt ctcataaccg gaaaacatca ccatcaccag caaaccgcca
    12661 accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag
    12721 aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac
    12781 gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt
    12841 tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa
    12901 ttgagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt cgcataattc
    12961 tgaggtttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag
    13021 gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aagacccgac cacgcagtat
    13081 tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc gaagatgaca
    13141 ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa
    13201 gcactggtga cagggggcga ttccggaata ggtcgcgctg ccgccatcgc ttacgcgcgt
    13261 gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc tcaggatgtg
    13321 aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga tttaagcgat
    13381 gagaaattcg cccgttcgct ggttcacgaa gcgcacaagg cgttaggcgg gctggatatt
    13441 atggcgctgg tcgccgggaa acaggttgcc attccggata ttgcagacct caccagcgaa
    13501 cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt tctggctaac ccaggaagcg
    13561 atccccctgc taccgaaagg tgcaagtatc atcaccactt cgtcaatcca ggcataccag
    13621 ccaagtccgc atttactgga ctatgcggct acgaaggcgg cgattctgaa ctacagccgt
    13681 ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc gccaggccct
    13741 atctggacgg cactgcaaat ttccggcgga caaacgcagg ataagatccc gcagtttggt
    13801 cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac tggcccctgt atatgtttat
    13861 ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggcgtgtg cggcggcgag
    13921 catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattg tgaattcact
    13981 cttacctgat gtggcgaaaa cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt
    14041 tgcaatagat tttgtaggcc tgatgagcgt agcgcatcag gcaattctac tttacttcgc
    14101 tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat gcagcgtatc
    14161 cattaccttc atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga agatggtggt
    14221 gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat cggtgaccgg
    14281 atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg gtttttccgg
    14341 gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta ccgtcgctaa
    14401 cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa acggcgtcac
    14461 gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg
    14521 acattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc
    14581 cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc
    14641 tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag gaatcgctgc
    14701 cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa cgactgccag
    14761 gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc agaccgtacc
    14821 aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt taccgcggcc
    14881 catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac gttctttaat
    14941 accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt cattgagcaa
    15001 atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct ggtttaagga
    15061 acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat tgaagaactc
    15121 tacgctctta ctgaagaaga ttgcccaagt gactacggag gccaaaataa gcccaatcat
    15181 cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa ggtccgtctg
    15241 catcaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct gcgcataata
    15301 atatcaaaat gacgtcgaat tgatagtcgt tctcattact atttgcatac tgccgtacct
    15361 ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa
    15421 ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct agtttagaca
    15481 tccagacgta taaaaacagg aatcccgaga tggcggacaa aaagcttgat actcaactgg
    15541 tgaatgcagg acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg attcagcgcg
    15601 cttcttcgct ggtctttgac agtgtggaag ccaaaaagca cgcgacgcgc aatcgcgcca
    15661 atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttctcctta caacaagcga
    15721 tgtgtgaact ggaaggtggc gcaggctgcg tgctatttcc ctgcggggcg gcagcggttg
    15781 ctaattccat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct
    15841 atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt
    15901 ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac actaaaatcg
    15961 tgtttctgga atcgccaggc tccatcacca tggaagtcca cgacgttccg gcgattgttg
    16021 ccgccgtacg cagtgtggtg ccggatgcca tcattatgat cgacaacacc tgggcagccg
    16081 gtgtgctgtt taaggcgctg gattttggca tcgatgtttc tattcaagcc gccaccaaat
    16141 atctggttgg gcattcagat gcgatgattg gcactgccgt gtgcaatgcc cgttgctggg
    16201 agcagctacg ggaaaacgcc tatctgatgg gccagatggt cgatgccgat accgcctata
    16261 taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc
    16321 tgaaagtggc tgaatggctg gcagaacatc cgcaagtagc gcgagttaac caccctgctc
    16381 tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc agcgggctat
    16441 tttcctttgt gcttaagaaa aaactcagta atgaagagct ggcgaactat ctggataact
    16501 tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc ctggcgaatc
    16561 aaccagaaca tatcgccgcc attcgcccac aaggcgagat cgattttagc gggaccttga
    16621 ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg gacgccggtt
    16681 ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt aattgtgaaa
    16741 agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag tacaataggc
    16801 agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca agatatcatc
    16861 gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt
    16921 tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc ctcatttttg
    16981 ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt tatggatttt
    17041 ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct aagttatatt
    17101 caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca gcttcctgct
    17161 aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc gctgctggct
    17221 ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat ttccggtctg
    17281 ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt cagcgtggta
    17341 accagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga agatcaggta
    17401 atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg
    17461 tttgtagtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt cccggctgct
    17521 gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat cttcccccgg
    17581 cgtcacaccg aagtaacgtt taaactcacg gctaaattgc aatgcgcttt catagtcgac
    17641 gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc gcgccttatg
    17701 cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact taaaattatg
    17761 atggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc tcaggttttc
    17821 ggtgtattta ttctcaatcc gtttcagcac gcggctaatc agactgaagt gagtctggcg
    17881 actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt acagaatttc
    17941 gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca cgtcgagtaa
    18001 ccgctccgcc gcgcataaaa tctcttctga taacgtggcg gagttaatcc cgctggctgc
    18061 catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct gtaactgcaa
    18121 aatatcgaca ttgagacgca atcctgccag cggcacctct gacgtggcat aggtttcgca
    18181 ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac gaaacacgcg
    18241 ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag gctcgtacat
    18301 caccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg gcaacagttc
    18361 tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca agcggcaaat
    18421 ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg attgtgcgca
    18481 attttgtagc atttctccag cactctggag aaataggcaa gacattggca gaaatgagca
    18541 ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt tctctgccct
    18601 catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca ccccaacccg
    18661 cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc acgatgctcg
    18721 cgtattgatt acctacggcg gcggcagcgt gaaaaaaatc ggcgttctcg atcaagttct
    18781 ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa acccggctta
    18841 tgaaacgctg atgaacgccg tgaaactggt tcgcgaacag aaagtgactt tcctgctggc
    18901 ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg ctaactatcc
    18961 ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta aaagcgccat
    19021 cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg caggcgcggt
    19081 gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg ttcagccggt
    19141 atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg tggctaacgg
    19201 cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg ttgatgccaa
    19261 aattcaggac cgtttcgcag aaggcatttt gctgacgcta atcgaagatg gtccgaaagc
    19321 cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc
    19381 gctgaacggt ttgattggcg ctggcgtacc gcaggactgg gcaacgcata tgctgggcca
    19441 cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc
    19501 actgtggaat gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg ctgaacgcgt
    19561 ctggaacatc actgaaggtt ccgacgatga gcgtattgac gccgcgattg ccgcaacccg
    19621 caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc tggacggcag
    19681 ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac tgggcgaaaa
    19741 tcatgacatt acgttggatg tcagccgccg tatatacgaa gccgcccgct aagcttttta
    19801 cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac acctcaccgg
    19861 agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac cgttattaag
    19921 ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc aagtaatgag
    19981 gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc
    20041 gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc agtcaacaga
    20101 gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc
    20161 ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt aatgcactgg
    20221 cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa
    20281 gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct gcaacgcctg
    20341 attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcatcc gctgatgcaa
    20401 caacgccagc tacacgcctg gaacgcgaca cacaaaatcc agaccgaatc ctggagccca
    20461 ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct ggcagataaa
    20521 tacggcaaag ccccggcgca gattgttatc cgctggcatc tggatagcgg cctggtggtg
    20581 atcccgaaat cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg ggatttccgt
    20641 ctcgacaaag acgaactcgg cgaaattgca aaactcgatc agggcaagcg tctcggtccc
    20701 gatcctgacc agttcggcgg ctaacatgca aattctcccg gtgacggtaa tgttccgcta
    20761 ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgttac tcttccttgt
    20821 tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta aaagaacaac
    20881 tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa taatacttct
    20941 gtttttagca gccctggcaa gttttaccgt acacgcgcag cctccttcac agaccgtaga
    21001 acaaacagtc cggcagattt atcagaacta taaatcagat gccagtacac cttatttcgg
    21061 tgaaaccgga gagcgggcga taacttcagc gcgtattcaa caggcgctta ccctgaacga
    21121 caatcttacg ctgccgggca atattggctg gctggattat gatccggttt gtgattgtca
    21181 ggattttggc gatctggtgc tagaaagcgt tgcgataacc caaactgacg ccgatcatgc
    21241 cgatgccgtt gtgcgctttc gtatctttaa agatgataaa gaaaagacca cgcagacact
    21301 gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag
    21361 cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctttag cttcgttgca
    21421 aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg attatagctg
    21481 gccgtggacg tgggtggttt ccgactctta ccgccaggcg gttaatgcct tctataaaac
    21541 caccttcaag acggccaata atcccgatga agatatgcaa atagaacggc aatttattta
    21601 cgacaatccg atctgttttg gcgaagagtc gctattttca cgcgttgatg aaattcgagt
    21661 cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga ccaatggcaa
    21721 caacgaagag caagaactgg ttttacagcg gcgcgaaggc aagtgggaaa tcgctgattt
    21781 tatccgcccg aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt
    21841 aaagcaatga gctgaattaa ataacaatta gccggaacaa taaataaaag ggaacactat
    21901 atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc
    21961 gtcaatgagc aaaaggtcaa tcaactggcg agcaatgtac aaacattaaa tgccaaaatc
    22021 gcccggcttg agcaggatat aaaagcacta cgcccacaaa tctatgctgc caaatccgaa
    22081 gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct gcgctgcttg
    22141 cgtatgtacg cagaatgata aaaaaatccc cggcagcatg tcagttgccg gggatttttt
    22201 ttaacgtcca accgccgctt tagggcgttt cttcgcacca gcattcaccg gacgagattg
    22261 cgtagacgac gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca tcggcgtatg
    22321 tttagtcaac gccggacggg tattgcggtt ctggcgacga gcttcacgca tctcttcaat
    22381 ggttggcgca ggcactaagc aatcgcgacg gctgccaatc agatgctttt tgcccatcgc
    22441 ttccagcgcc gggcggatta acggccagtt tgccggatcg tggtaacgca acaacgcttt
    22501 atgcaaacga cgctgtttgt cgcccttcgg tacgaagacg tcttcgctct tataaccaat
    22561 cttcgccagc ggttttttcc cggtgtaata catggtggtt gagttcgcca gcggcgatgg
    22621 gtagaagttc tgtacctggt cgagacggaa acgatgcttt ttcagccaca gcgccaaatt
    22681 caccatatct tcatcacgcg taccggggtg cgcagagatg aaatacggga tcagatactg
    22741 ctctttacct gcctgtttcg agtaggtatc gaacagctct ttaaagcggt catagctgcc
    22801 catgcccggc ttcatcatct tcgacaacgg cccttcttcg gtatgttccg gagcaatctt
    22861 cagataaccg ccgacgtgat gggtcgccaa ctctttgata tagcgcggat cttccacggc
    22921 gatgtcataa cgtacgcctg aggcgatgag gattttttta atgcctttca gatcacgcgc
    22981 acggcgatag aggttgatcg tcggttcgtg gttagtgtcc atgtgcggac aaatatccgg
    23041 ataaacgcac gacaaacggc gacaagtttg ttcagcgcgt ggcgatttgc agcgcaacat
    23101 atacatgttg gcagttggcc caccgagatc ggaaatcacg cccgtaaaac ctggaacggt
    23161 gtcgcggatc gcttcgatct cattaatgat cgaatcttcc gaacggctct gaataatgcg
    23221 cccttcgtgc tcggtaatag aacagaaaga acagccgcca aagcagccgc gcataatgtt
    23281 gaccgaaaaa cggatcattt cgtaagccgg aatacgggca ttgccatagg ccggatgtgg
    23341 cacgcgcttg tacggcagcg caaaaacgct gtccatctct tcggtagaaa gcgggatagc
    23401 aggcgggttg atccacacat agcggtcacc gtgtttttgc atcaatgcgc ggacgcagcc
    23461 tgggttggtt tcgtggtgca gaatacgcga agcatgggcg tacagcactt tatcgccctt
    23521 cactttctcg aaagaaggca gcaacacgta ggttttttcc cacggtttcg ggcgcggtgg
    23581 ctgcacggtt acggctttgg cttcctgctt tttcggtgcc accggtttgt tatccgcgca
    23641 cggcaaatct tcaccatacg gatgcgggat tgggtcgatt tttccagggg tatcaagacg
    23701 ggtggaatcc acgccgctcc agccaggcag cgcctctttc acgataatcg cggtattacg
    23761 cacatcgcgg atttcactaa tcggctcgcc catcgccaga cggtgcgcca cctccaccag
    23821 cggacgctca ccgttaccaa acatcagcat gtcggctttc gaatccacca gcacggaacg
    23881 gcgcacggta tcggaccagt aatcataatg tgcggtacgg cgcagactag cctcaatacc
    23941 gccgaggatc accggtacat ctttccacgc ctctttacaa cgctgggtat aaaccagtgt
    24001 ggcgcgatcc gggcgcttac ccgcgacgtt atccggcgtg taggcatcgt catgacgtaa
    24061 acggcgatcg gcggtataac ggttgatcat cgaatccatg ttgccagcag taacaccgaa
    24121 aaacagattc ggtttaccca gacgcataaa gtcgtctttg ctgctccagt ccggctgggc
    24181 gatgatcccg acgcgaaagc cctgtgcttc cagcatacga ccgcaaatcg ccatcccgaa
    24241 gcttgggtga tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc
    24301 aagttgatcc atctcttcac gagacatcgg caaaaacggt gccggtccaa aacaggcggc
    24361 ccagtactgc ggccaggaga acaggtcgcg atccggttgg atcagggaga tagagctcat
    24421 tttgcttcca gaaatgataa aaaaataatc aaaggccggg gattataagc cggaacgaaa
    24481 gagaaatcga aaagtattcc atactcgccc tcctcgggcg agtatgaaga ttacggtacc
    24541 ggattgacta acagttgccc aatcgagcca cggtccgcca tttccagcgt ctgactgttg
    24601 aagtagaacg ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt
    24661 ccgtcaaccc aaacggtatc tttccagcct ctgtcttccg ggaacggcat cgcaccattc
    24721 acgttacgga tctggaacat cacgccttca atatggaacg cttgcggctc gtccgcgcgt
    24781 accgtccagc gttcccacgt tccttgctgc gcggtgacat caatacggtt gacgtcccac
    24841 agctgtccat taatacccgg gtcatcaccc agactgatat cgcgactgcg aatcggcgaa
    24901 ccggccatga tttcagttgg cagcaagcgc atcggaagac tgtctgtgac cagcggcaga
    24961 aggccggttg ggcgcagcgt tagcaccagg gtagaaacca gaatactgga tggctcaaag
    25021 aagccgcgaa tacgatcaac aatgctcgcc gcttcgccac aggtgatcga cacttcatcg
    25081 ccgttgctca tatccaccag aatctcgcgg cgctcgcccg gtgccagcga aagttgcttc
    25141 accgatacag gagcaggcag gaatccctga tcgccagaaa tcacatgtaa cgggcgacca
    25201 tcgctcatct gtagttgata gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc
    25261 cagccacgcg agacttcaac gtacgggctt tgtacaccgt taaccagcag cgtatcacca
    25321 acaaagccgc cgcttcccgg ttcgttgtat tctggcgtac caaagttatc cagccgttta
    25381 tcctggataa tgaccggaaa atcatccaca tcataatggt tggggatagg cagcgacttg
    25441 ctgacttcat cttccaccag ccacattccg gcaaggccgt tatagacctg ctgagccgtg
    25501 cggttgggag tattggcgtg ataccacaga gtagctgcgt tctggcgaat aggcagtacg
    25561 ggtgcccagt cagcgtttgg cgacatcatc cgtgccggac cgcccatcag cgggccaggt
    25621 acctgtagcc cggccaccgt cattgagaca ttttctgtca ggcggttgct gtaaataagc
    25681 ttaacatcgt cgcccttcca gacgcggata gtcggcccca ggtaacgacc attgattccc
    25741 cagaccgacg cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac agtcataaac
    25801 agcggttgcc cacggcgaga ttcaagtagc ggcggaacgg gtagcggttg ctgttgcccg
    25861 gctgcgctgg ccttcagggg aacagcgcct gcacaaagtg caatccccga tgcctgaatg
    25921 aactgacgcc gactgagtga catatttgct ccatgtaaaa ctggctaaag taataccgtg
    25981 caggaaagtg ttccccgcaa aaatacagat tgccgactta aacttttccg gcggcttcgc
    26041 gttctgcgac ttctttatcg agctcggcga ttttttgttc cattatcgaa cgacaatggg
    26101 cagccagctc acgaacctga tctttgccat actgactgac gtcaattggc ggcagcattt
    26161 cgacaatcac cagaccgttg tgcagtcgat taagattaat cttattcgaa gttgtagaga
    26221 cgcacacggg aataatcggg acgcccgccg caattgccgc gtgaaatgct ccagtcttga
    26281 acggtagcag gccgcgacca cggctgcggg ttccttccgg gaacatccag atggaaatac
    26341 ggcgtttttt gaaatgattc acgacttccg caatggtgcc gtgagcttta gtgcgattgt
    26401 ttctgtcgat caataagttg ccggttaacc agtacaactg cccgaagaag gggatccaca
    26461 gcaagctctt tttacctacc gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca
    26521 tgtcatagtt gttctggtgg ttagcgatat agatagcatt gccgtagctt tcagcatctg
    26581 caggtttgcg gcactcaact ttcaggccga acagcggcgc aagacggcca aacatatgcc
    26641 caaaggtggc cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg gagccgaata
    26701 cacagactaa gatgctgtaa atcacggtaa taataagacg aaagatatat agcataacac
    26761 cctctgaagg tctgagagac tggcattgta cgtcacctga aatcaggtta gggcattgtt
    26821 aaaagccgct ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa caacggtttc
    26881 aagggaattt ttatcgtttc cgggggttga atacgctgcc ggatgacgac ttaacgtttc
    26941 atccggcgtt ccttgcaagc gggaggaaac aggccctccc cggcatatta ctcttcgcta
    27001 tcaccgttgc tggcacggcg aggagagtcg atctcaacac gatcgatacg ctgcaaaccg
    27061 cgcatcaacg taccgcggcg tccacgttca ccagtgactt tctgtaactc ttccgggcgc
    27121 agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg cagaacgtac
    27181 aattgcgcca gaccatcttc tccacgcgcg gcttctgccg atggaatgtt gataatcttg
    27241 ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa catacggcct
    27301 gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg cggcataaca
    27361 tgggcatttt ccggtaaggt gatcaaagcc ttacctgcac ggttacgcgc caccagatcg
    27421 ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt ctgatcgtcg
    27481 ctttccatca gcatatggtc aacggtcgcc ccaggcggca acgttaattt gccggtgagt
    27541 ggttcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata gctacgaccg
    27601 gtggaatcaa caaacactac cgattggttg ctcttacctt tcaccgccgc tttgaagcta
    27661 tcacccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc gctgcgtacc
    27721 cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc gtgctcgctc
    27781 atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gatcgtcacc gtaggcttgc
    27841 gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga agccaaaatg
    27901 ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat cttcatctct
    27961 tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg ggtttccgta
    28021 aggccaaacc gcgacatcag cgccggtttc ggttcatctt cgttacgaat gatctcaatc
    28081 acttcgtcga tgttgagaaa cgccaccagc aaaccttcca ggatatgcag gcgcttgagg
    28141 actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcgacggaa caccagccat
    28201 tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc gatcatattg
    28261 aggttaatgc gatagctctt ttccagatcg gtggtagcga agaggtggtt catcacctga
    28321 tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt ctcgtggtca
    28381 gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg cgcagcaatt
    28441 tgctccagta cacgcgcacc tgaaacctga tgcggtaatg cgctgataac caccgcgcca
    28501 tcttctttct tccacaccgc gcgcatacgc actgaaccac gtccgttctc gtagatttta
    28561 cggatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg cccctgcacg
    28621 atatccagca gctgatcgag cgtggttttc ggctggtcga ttaatgcgat tgccgcctga
    28681 gctacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc aataccggtg
    28741 gtgccgttaa gcaaaatgtt tggcagacga gcaggcagca ttttcggctc ctgcaaagtg
    28801 ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa tagcagctcg
    28861 gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt cggatcgtcc
    28921 ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa cggctgcgcc
    28981 atcaggacca tcgcttcata acaggcgcta tcgccgtgcg gatggtattt acccagtacg
    29041 tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc cagttcagac
    29101 atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa cggcaacgca
    29161 cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa ttcatgtagc
    29221 gcaaggcgct ctgccatatc gctcattaat tctgattcct caactgattc gccagccttc
    29281 aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac aggagatcag
    29341 cgtgaatagc gccgccggac gtgctccgac ggcgataaag gcatcaatgt gccttgatat
    29401 acaactcttt gctgtagact tgatcggcat agagggcagc acaaggttgc gcccatgcca
    29461 caccactcgg catgggggtc cgctccgggc gattcgagaa gttgaggtta tgcgagtcca
    29521 gggaagccta aagcatcaaa gtagtcgact gtaagtatat ggttcagccc agtttagctg
    29581 tgtctccacc agtgccccgc gcctcctgcc ataagtgcgg cttgctcatg ggtacccccg
    29641 agacggctta cggcgcaccg gataataaat ttcgcgtata catggtattc cttaccggag
    29701 aaattataag cccaacaaca atacgcctgt ggcattgttc gctcaaactt atcgcgagtg
    29761 atttggctca cactccggta aagattcccc atcacgctaa cggcacataa acatccgttt
    29821 ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta gtgaaacaga atcggtgctt
    29881 tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat aatgccccat atcgtgtgtg
    29941 aaatatcgtc cagctcgcca acgtggcggg ccacggcata acgtccaccg gtaagttcac
    30001 cattgctcac accataacga ttatcgggaa tcggttcgct aacgctgccg cagatatcaa
    30061 agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca ggcgacgcca aaagtctggc
    30121 tttggttaac aggagaaagc cccgtctctt tacgccacat gataaatttc gccacactgt
    30181 agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc cacagggatt gatgggaaat
    30241 cgataatgtt tacatccagt gtcaggtttg tcattttgcc tccctgcgaa attattcccg
    30301 ggaacgcgtc ccgggagcgg taacaattac ggatttactt tgcggatctg tttgacgtca
    30361 atttcaacag aattccagtc tttatcgact tcaccctgaa tctcaaccgt atctttcggc
    30421 gtcaccgtca caccgttcca gcgtttgtgg tcgatatcaa cattgatagt accgctggca
    30481 tctttaaaca cgtagagatc gtcagagatg cgttcaacga tattgccgcg cagggttacc
    30541 caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg ttacgctgcc gttcggcccc
    30601 tggaatcctc cggcctgact ttgcgttgcc gatgggccag aaaaaccgcc ctgctctgct
    30661 gccatcaccg gtgcactgca cagggccatt actgcgatta ctgctgcgaa ttttttcatg
    30721 tttattactc cctttaatgt ctgtttccga gcatttaaca agatagtcct taacaacttc
    30781 ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg ttacaacacg gtttactggc
    30841 agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt tactgataga agatgacatg
    30901 ctgattggcg acggcatcaa aactggcctt agtaaaatgg gttttagtgt cgactggttt
    30961 acacaaggtc gtcagggaaa agaggcgctt tatagcgcac cttatgatgc ggtgatcctg
    31021 gatttaacct taccaggcat ggatggtcgc gatattttgc gcgaatggcg agagaaaggt
    31081 cagcgtgagc cggtactgat cctgaccgcg cgtgatgcgt tagcggaacg tgtagaaggg
    31141 ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg cgttgataga agtcgccgcc
    31201 aggctggagg ctctgatgcg ccgaaccaac ggccaggcca gcaacgagct gcgccacggt
    31261 aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg ctggcgaacc cttaacactg
    31321 aaaccaaaag aatttgccct gctggaatta ctgatgcgta acgctggtcg ggtactgccg
    31381 cgcaaactga ttgaagagaa actgtatacc tgggacgaag aggtcaccag taatgccgtt
    31441 gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg attttattcg taccgtgcat
    31501 ggtattggct acacattagg tgagaaatga aatttaccca acgtcttagt ctgcgcgtca
    31561 ggctgacgct aatcttttta attctggcct cggtgacctg gctgctttcc agctttgtcg
    31621 cctggaaaca aacaacggat aacgtcgatg aattgttcga cacccaactg atgctgtttg
    31681 ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc ggcggatcgc atggcacaga
    31741 cgccaaataa attaaaacac ggtcatgttg atgacgatgc gctgaccttt gccatcttta
    31801 cccacgacgg cagaatggtc cttaatgatg gcgataacgg agaagatatt ccctatagct
    31861 atcaacggga aggttttgct gacgggcaac tggtcggtga agacgatcct tggcgttttg
    31921 tctggatgac ctcacctgat ggcaaatatc gcatcgttgt tggccaggaa tgggaatacc
    31981 gtgaagacat ggcgctggcg attgttgccg ggcaattgat cccgtggctg gtcgcactgc
    32041 cgattatgtt aatcatcatg atggtactac tgggtcgtga actcgcgccg ctgaacaaac
    32101 tggcgctggc actacgtatg cgtgaccctg actcggaaaa accactaaac gcgactggcg
    32161 tacccagcga agtgcgacca ctggttgagt cgctaaatca actgttcgcc cgcacacatg
    32221 cgatgatggt tcgagaacga cgctttacct ccgatgcagc tcacgaacta cgtagcccgt
    32281 taacagcgct gaaagtgcaa accgaagttg cgcagctctc tgacgatgat ccgcaggcgc
    32341 ggaaaaaagc actgctccaa ttacattccg ggatcgatcg cgctactcgt ctggttgatc
    32401 aactgctcac gctatcgcgg ctggactcac tggataacct tcaggacgtc gcggagatcc
    32461 cgcttgaaga tctcctgcaa tcgtcggtga tggatattta ccacacggcg cagcaggcga
    32521 aaattgacgt gcgactgaca ctcaatgccc acagcatcaa acgcactgga caaccgctat
    32581 tgctaagttt gttggtgcga aaccttctgg ataacgccgt gcgctacagt ccacagggca
    32641 gcgtggtgga cgtcacgctg aatgccgata atttcatcgt gagggataac ggccccggtg
    32701 tgacaccaga ggcactggcg cgaattggcg aacgcttcta tcgccccccc ggacaaaccg
    32761 ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaaattg catggcatga
    32821 atgttgaatt tgggaatgcg gaacaaggtg gatttgaggc gaaggtgagc tggtaagggt
    32881 aagacttttg ctaaattcgc aaaagacttt gcacattttg ctaattttac cgtaccgctc
    32941 tgtgacgtac tatagtcgac aaacgtctca ccttgaggtt aaaaaatgag caacaccctg
    33001 attatcaacg gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc
    33061 gaagtcgcgg atggcacact gcgcgacctc gggcatgatg ttcgcatcgt tcgcgccgac
    33121 agcgactacg atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg
    33181 cagatgccag gctggtggat gggcgcgccg tggacagtga aaaaatacat tgatgatgta
    33241 ttcaccgaag gtcacgggac gctgtatgcc agcgatggcc gtacccgcaa agatccgtca
    33301 aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg
    33361 aacgcaccaa tggaagcctt caccgaaaaa gatcaattct tccacggcgt tggcgtcgac
    33421 ggtgtgtatc tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca
    33481 tttatcgcta atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc
    33541 aagcatcttg tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg
    33601 cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg
    33661 gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg
    33721 ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg
    33781 atgattgagc agtgggaaag catcgcgcac cttgaagcgc atctgcaaac cccgcacatg
    33841 aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga tgaatattcg tattctgcag
    33901 ccagggattt aatcctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt
    33961 taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag
    34021 cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg
    34081 atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc
    34141 gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt
    34201 aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg
    34261 agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg
    34321 cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc
    34381 gcatccgcga ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga
    34441 tcgataccga tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa
    34501 gaaacttccc aggtgttaag gatcttacct ttcagcggca tgatcgcctg atattcacga
    34561 tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacgaggaa cagttcggta
    34621 cggttaaggt cctgcgcggt acaatcagcc agtttgccag gcagcgccgg gccgctggtc
    34681 agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct ggaaatcgcc
    34741 atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat aaaggcatct
    34801 ttcaccacgc cagaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttcgtctgc
    34861 ccggcaaact gcggatcctg cattttcacc gacagaacat aggcgcagcg atcccagata
    34921 tcttccgccg acagcttcac gccgcgcggc agaatgttgc gatattcaca gaactcacgc
    34981 atcgcgtcca acaggccctg acgcaggcca ttaacatggg taccgccctg catcgttggg
    35041 atcaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc
    35101 cagtccacag cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc
    35161 ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc
    35221 tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtaccgct
    35281 ttggctttca gcacatgcgt caggcgtgaa acagaaaatc gcgggctgtc aaagaaggtt
    35341 tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc
    35401 tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg
    35461 cgcacgttaa cttcaacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc
    35521 aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa
    35581 agaatcaatt caaccgccgg gacaccctct tccgggtgaa tatccaccgg catcccgcgc
    35641 ccatcgtcaa taacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg
    35701 tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg
    35761 cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc
    35821 tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg
    35881 tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt
    35941 gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc
    36001 ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc
    36061 ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata
    36121 ttgctgcccg gtgtatgggt tctcgttctg accgagatag tccgtcagca gttcaaacgg
    36181 gcgcaccgcc gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc
    36241 gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac
    36301 aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat
    36361 ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg
    36421 cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg
    36481 gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca
    36541 gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa gttggaacag
    36601 tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg
    36661 agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc
    36721 agcacggtat ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc
    36781 gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt
    36841 ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc
    36901 tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt cgccggggag
    36961 atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc
    37021 cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag
    37081 gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctgatgtgga
    37141 cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagagcttcg
    37201 tgcttttgtg caaacaggtg agtgtcggta atttgtaaaa tcctgactct ggcctcacca
    37261 gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg atgctagtaa
    37321 accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactga
    37381 ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct
    37441 ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga
    37501 cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc
    37561 accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc
    37621 tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa
    37681 aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat
    37741 gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac
    37801 gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca
    37861 tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg
    37921 tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa
    37981 cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg
    38041 gcaacatctt ccacactttc accctcttct atcatcccgg caaccatctc cagtagccag
    38101 ggggtttcac tggtgtcgta tgcggcaatc cgaatctgct caatcagcac aacttcatcg
    38161 cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt
    38221 acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa
    38281 aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta
    38341 acgggcaggt tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg
    38401 tgaatttcag cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta
    38461 acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat
    38521 aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat
    38581 tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga
    38641 acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg
    38701 ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc
    38761 taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact
    38821 ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc
    38881 gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc
    38941 agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg
    39001 ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc
    39061 aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcgcagtacg
    39121 ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc
    39181 tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc gaaaacttta
    39241 aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt
    39301 cgctgttaca ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg
    39361 atggtcactt accgacgctg gatttaacgg cttctaccgg gatttctgac acctcttata
    39421 gcggttcgaa aacccgtggt gccgctggta cccagtatga cgacagcaat atgggccaga
    39481 acaaagtggg cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg
    39541 tgaaacaggc ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta
    39601 gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcatctatc agtagcatta
    39661 acgcctacaa acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct
    39721 actcggtcgg tacgcgtacc attgttgatg tgttggatgc aaccaccacg ctgtacaacg
    39781 ctaagcaaga gctggcaaat gcgcgttata actacctgat taaccagttg aatattaagt
    39841 cagctctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac
    39901 cggtttccac taatccggaa aacgttgcac cgcaaacgcc ggaacagaat gctattgctg
    39961 atggttatgc gcctgatagc ccggcaccag tcgttcagca aacatccgca cgcactacca
    40021 ccagtaacgg tcataaccct ttccgtaact gacgacgacg acggggcttc ggccccgtct
    40081 gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca
    40141 atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa
    40201 tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc
    40261 tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg
    40321 atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa
    40381 gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga
    40441 aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac
    40501 cagcccaggc tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt
    40561 tctggatgcc gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat
    40621 ttgcacagca gccgctgttc tcctcgaaaa acccagccag cccggcttac ggtaaatata
    40681 ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga
    40741 agacagccat ggcaccaaaa ccggcgacca ctactaccgt tacccgtggc ggttttggtg
    40801 aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct tctcgttcaa
    40861 tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgcgaaaa
    40921 agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga
    40981 tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact
    41041 gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa
    41101 attccgcatt ccaaaacgca cctggagttt tgtgcgtcag tcatggctga ctcaccagcc
    41161 atcgctttat tcgcgtcttg atctggcatg ggatggcact ggcgaaccta aactgctgga
    41221 aaataacgcc gatacaccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct
    41281 ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca
    41341 agaaaagcta atcgatcgct ttgtcgagtt gcgtgaacag tatggcttcc agttgctgca
    41401 tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg
    41461 cgcgacggaa gctgaaatcg ctaccgagtt cctctacatt gacgatatcg ggttaggtga
    41521 aaaaggtcag ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc
    41581 atgggaattt atgttgcgtg agatgttctc caccaagctg gaggatgcag gcgtacgctg
    41641 gctggaaccg gcgtggaaga gcattatctc caacaaagcg cttctaccgc tactgtggga
    41701 gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca
    41761 aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat
    41821 tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt
    41881 tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct
    41941 ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcgttga tcacccagga
    42001 tatgtctcgg ttttatccac atatttttat tgagtaatca atgatgccgg atggcattcg
    42061 ccatccggta attgttaacc tatctgcacc gacagcatac tcaggctgcc catttctata
    42121 ccatcgaccg gaatggtaat tggctcctgc ccatcccacg cacctaagac atacaacaac
    42181 ggcagatagt gctccggcgt tgggttcgat aacgcgccac cttcatggtc aaggtaattc
    42241 accagaggat gctgctctac cgccccttgc cacgtcagat tcgctttcac atactcatta
    42301 aacgacatcg cccaaggata tggtgaacta tcaccgtgcc acttcactgt acgcaggtta
    42361 tgcaccacgt tgccactggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg
    42421 cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc
    42481 gggatatcgg cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc
    42541 caggcttctt tatccagcgt caccgggacc gacgctaaca gctcaaccag atgctgtgcc
    42601 agcacaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca
    42661 aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag
    42721 tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag
    42781 ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat
    42841 gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc
    42901 tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt
    42961 attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc
    43021 attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg
    43081 caaaaaccct cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg
    43141 ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg
    43201 ttgggttatg gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg
    43261 ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc
    43321 aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt
    43381 gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc
    43441 gcgttgcaca atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg
    43501 tctaaacgca ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt
    43561 gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg
    43621 gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa
    43681 gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga
    43741 ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc
    43801 agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc
    43861 ttctctcgta ctgtttaagt atttgttccg cg
//
LOCUS       DAZPJP010000013        43213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPJP010000013 DAZPJP010000000
VERSION     DAZPJP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.013"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..515
                     /locus_tag="ACHU9J_000651"
     CDS             <1..515
                     /locus_tag="ACHU9J_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1904285.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            531..1070
                     /locus_tag="ACHU9J_000652"
     CDS             531..1070
                     /locus_tag="ACHU9J_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1904286.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1165..2742)
                     /gene="guaA"
                     /locus_tag="ACHU9J_000653"
     CDS             complement(1165..2742)
                     /gene="guaA"
                     /locus_tag="ACHU9J_000653"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1904287.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2811..4277)
                     /gene="guaB"
                     /locus_tag="ACHU9J_000654"
     CDS             complement(2811..4277)
                     /gene="guaB"
                     /locus_tag="ACHU9J_000654"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1904288.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4439..5809
                     /gene="xseA"
                     /locus_tag="ACHU9J_000655"
     CDS             4439..5809
                     /gene="xseA"
                     /locus_tag="ACHU9J_000655"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1904289.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5806..6021)
                     /locus_tag="ACHU9J_000656"
     CDS             complement(5806..6021)
                     /locus_tag="ACHU9J_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1904290.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6090..7562)
                     /gene="der"
                     /locus_tag="ACHU9J_000657"
     CDS             complement(6090..7562)
                     /gene="der"
                     /locus_tag="ACHU9J_000657"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1904291.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7680..8858)
                     /gene="bamB"
                     /locus_tag="ACHU9J_000658"
     CDS             complement(7680..8858)
                     /gene="bamB"
                     /locus_tag="ACHU9J_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1904292.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8869..9489)
                     /gene="yfgM"
                     /locus_tag="ACHU9J_000659"
     CDS             complement(8869..9489)
                     /gene="yfgM"
                     /locus_tag="ACHU9J_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1904293.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9507..10781)
                     /gene="hisS"
                     /locus_tag="ACHU9J_000660"
     CDS             complement(9507..10781)
                     /gene="hisS"
                     /locus_tag="ACHU9J_000660"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1904294.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10892..12010)
                     /gene="ispG"
                     /locus_tag="ACHU9J_000661"
                     /gene_synonym="gcpE"
     CDS             complement(10892..12010)
                     /gene="ispG"
                     /locus_tag="ACHU9J_000661"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1904295.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12037..13050)
                     /gene="rodZ"
                     /locus_tag="ACHU9J_000662"
     CDS             complement(12037..13050)
                     /gene="rodZ"
                     /locus_tag="ACHU9J_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1904296.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13335..14489)
                     /locus_tag="ACHU9J_000663"
     CDS             complement(13335..14489)
                     /locus_tag="ACHU9J_000663"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1904297.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14639..15070)
                     /gene="ndk"
                     /locus_tag="ACHU9J_000664"
     CDS             complement(14639..15070)
                     /gene="ndk"
                     /locus_tag="ACHU9J_000664"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1904298.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15219..17531)
                     /gene="pbpC"
                     /locus_tag="ACHU9J_000665"
     CDS             complement(15219..17531)
                     /gene="pbpC"
                     /locus_tag="ACHU9J_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1904299.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17532..22493)
                     /gene="yfhM"
                     /locus_tag="ACHU9J_000666"
     CDS             complement(17532..22493)
                     /gene="yfhM"
                     /locus_tag="ACHU9J_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1904300.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22700..23545
                     /gene="sseA"
                     /locus_tag="ACHU9J_000667"
     CDS             22700..23545
                     /gene="sseA"
                     /locus_tag="ACHU9J_000667"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1904301.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23881..24006
                     /gene="timP"
                     /locus_tag="ACHU9J_000668"
     CDS             23881..24006
                     /gene="timP"
                     /locus_tag="ACHU9J_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1904302.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24038..24814)
                     /gene="sseB"
                     /locus_tag="ACHU9J_000669"
     CDS             complement(24038..24814)
                     /gene="sseB"
                     /locus_tag="ACHU9J_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1904303.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24956..26239)
                     /gene="pepB"
                     /locus_tag="ACHU9J_000670"
     CDS             complement(24956..26239)
                     /gene="pepB"
                     /locus_tag="ACHU9J_000670"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1904304.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26417..26617)
                     /gene="iscX"
                     /locus_tag="ACHU9J_000671"
     CDS             complement(26417..26617)
                     /gene="iscX"
                     /locus_tag="ACHU9J_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1904305.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26629..26964)
                     /gene="fdx"
                     /locus_tag="ACHU9J_000672"
     CDS             complement(26629..26964)
                     /gene="fdx"
                     /locus_tag="ACHU9J_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1904306.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26966..28816)
                     /gene="hscA"
                     /locus_tag="ACHU9J_000673"
     CDS             complement(26966..28816)
                     /gene="hscA"
                     /locus_tag="ACHU9J_000673"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1904307.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28833..29348)
                     /gene="hscB"
                     /locus_tag="ACHU9J_000674"
     CDS             complement(28833..29348)
                     /gene="hscB"
                     /locus_tag="ACHU9J_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1904308.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29444..29767)
                     /gene="iscA"
                     /locus_tag="ACHU9J_000675"
     CDS             complement(29444..29767)
                     /gene="iscA"
                     /locus_tag="ACHU9J_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1904309.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29784..30170)
                     /gene="iscU"
                     /locus_tag="ACHU9J_000676"
     CDS             complement(29784..30170)
                     /gene="iscU"
                     /locus_tag="ACHU9J_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1904310.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30198..31412)
                     /gene="iscS"
                     /locus_tag="ACHU9J_000677"
     CDS             complement(30198..31412)
                     /gene="iscS"
                     /locus_tag="ACHU9J_000677"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1904311.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31653..32141)
                     /gene="iscR"
                     /locus_tag="ACHU9J_000678"
     CDS             complement(31653..32141)
                     /gene="iscR"
                     /locus_tag="ACHU9J_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1904312.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32442..33179)
                     /gene="trmJ"
                     /locus_tag="ACHU9J_000679"
     CDS             complement(32442..33179)
                     /gene="trmJ"
                     /locus_tag="ACHU9J_000679"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1904313.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33298..34101
                     /gene="suhB"
                     /locus_tag="ACHU9J_000680"
     CDS             33298..34101
                     /gene="suhB"
                     /locus_tag="ACHU9J_000680"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1904314.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34246..35100
                     /locus_tag="ACHU9J_000681"
     CDS             34246..35100
                     /locus_tag="ACHU9J_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1904315.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35291..36571
                     /gene="csiE"
                     /locus_tag="ACHU9J_000682"
     CDS             35291..36571
                     /gene="csiE"
                     /locus_tag="ACHU9J_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1904316.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36563..37702)
                     /locus_tag="ACHU9J_000683"
     CDS             complement(36563..37702)
                     /locus_tag="ACHU9J_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1904317.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37862..38752)
                     /gene="hcaR"
                     /locus_tag="ACHU9J_000684"
     CDS             complement(37862..38752)
                     /gene="hcaR"
                     /locus_tag="ACHU9J_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1904318.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38888..40249
                     /gene="hcaE"
                     /locus_tag="ACHU9J_000685"
     CDS             38888..40249
                     /gene="hcaE"
                     /locus_tag="ACHU9J_000685"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1904319.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40246..40764
                     /gene="hcaF"
                     /locus_tag="ACHU9J_000686"
     CDS             40246..40764
                     /gene="hcaF"
                     /locus_tag="ACHU9J_000686"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1904320.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40764..41084
                     /locus_tag="ACHU9J_000687"
     CDS             40764..41084
                     /locus_tag="ACHU9J_000687"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1904321.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41081..41893
                     /gene="hcaB"
                     /locus_tag="ACHU9J_000688"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41081..41893
                     /gene="hcaB"
                     /locus_tag="ACHU9J_000688"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1904322.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41903..43105
                     /gene="hcaD"
                     /locus_tag="ACHU9J_000689"
     CDS             41903..43105
                     /gene="hcaD"
                     /locus_tag="ACHU9J_000689"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1904323.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10248 a        11944 c        10852 g        10169 t
ORIGIN      
        1 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
       61 gccagtcaaa tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      121 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      181 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      241 gtgttatcgg ccacaacgtc ggttctggca gcaattccgg aacgacggca ggggcagttg
      301 gcggcggagc tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag
      361 gtgtttcttt aacatataag gaaggcacca aagtgtatac ctccacccag gtgggtaaag
      421 agtgccagtt tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta
      481 ttcagccaaa tactaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      541 tttttctttg cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg
      601 atcaactctc tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc
      661 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      721 atgcccaggc cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      781 aggataaact ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg
      841 agctacgcag cttagagatt cagaaacaaa aactgacgct ggcgaaagaa gaagcccgcg
      901 ttaagcgaga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
      961 tggtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1021 aaagcgtggg caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttag
     1081 taacttcaag cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1141 tcattcatag tgcagggatt aaaatcattc ccactcaatg gtagctggcg gcttgccgct
     1201 gatgtcatac accacgcggg aaataccgtt cacttcattg ataatgcggt tggaaacgcg
     1261 accgaggaaa tcgtacggca gacgcgccca gtgtgcggtc ataaagtcga tggtttcgac
     1321 agcacgcaga gagacaaccc agtcatactt acgaccatcg cccattacgc caacggaacg
     1381 taccggcagg aacacagtga acgcctggct gactttgtcg tacaggtccg ctttacgcag
     1441 ttcttcaatg aagatggcgt cagcacggcg cagcaggtca cagtactctt tcttcacttc
     1501 acccagcaca cgaacgccaa ggcctggtcc cgggaatggg tgacggtaca acatgtcgta
     1561 cggcaggccc agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg
     1621 ttcaaccagg cccatcttca tctctttcgg caggccgccc acgttgtggt gagatttgat
     1681 gacgtgtgct ttaccggttg cagacgccgc agattcgata acgtcagggt agatggtgcc
     1741 ctgcgccagc cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa
     1801 aacgcgaccg atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacag
     1861 gaagcgatct tctgccggaa cgtgaacaat gttcagacca aagtgatcgc caaacatatc
     1921 cagaacctgc tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag
     1981 gtttttaccg atagcgcggt gcagtagcat tgcggttacg gaggaatcca cgccaccaga
     2041 gaggccgagg atgactttat cgtcgcctac ctgctcacgg atgcgggcta cagcatcgtc
     2101 gataattttc gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc
     2161 cagcatgcgc ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa
     2221 gcgtttttct tcgttagcca taatggcaaa cgggcagctt tcggtgctgg ctacggtgac
     2281 gaagtcggac gggatagcgg taactttatc gccgtggctc atccagacat cgagcagcgg
     2341 tttaccgtct gcggtcagcg catcttcgat accgcgaacc agtgcactgt cgtttacgac
     2401 ttcaacctgc gcgtagccaa attcacgttc gttagaggct tcaacgtgac cgcccaactg
     2461 cattgccatg gtctgcatgc catagcaaac gccgaatacc ggtacgcctg cttcaaagac
     2521 atactgcggc gcacgcgggc tgttttcttc agtagtactt tccgggccgc cggaaagaat
     2581 aatgccgctt ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc
     2641 gcagtaaaca cccagctcac gcacgcggcg cgcaaccagt tgagtgtact gagaaccgaa
     2701 gtccagaatg aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc
     2761 aagtgaaaca gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc
     2821 agacggtagt tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaatg
     2881 cccgcaccgc tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag
     2941 ccggtcagac ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg
     3001 cgacctttat aagctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgtta
     3061 tcgctctgga aataacggtc agaggaacct ttggacatcg cgcccaggga acccatacca
     3121 cggtaagatt tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta
     3181 cccgccagca tggaacctac catcaccgcg cttgcgccag cggcgatagc tttggcgatg
     3241 tcgccggaga agcgaatacc accatctgcg ataaccggaa taccggtgcc ttccagggct
     3301 tcaactgcgt cagcaacggc agtgatctgc ggaacaccta cgccagtaac gatacgagtc
     3361 gtacagatag aaccagggcc gataccgact ttaaccgcgc tgcaaccagc ttctgccaaa
     3421 gcgcgtgcac ctgcagctgt tgccacgttg ccgccgatga tttgcagatc cggatattta
     3481 gcacgggttt cacggatacg ctgcaaaacg ccttctgagt gaccgtggga ggagtcgatc
     3541 agcagaacgt caacgccagc ggcaaccagc gcgtcaacac gctcttcgtt acccgcacct
     3601 gcgccaactg cagcaccaac acgcagacgg ccttgctcgt ctttacaggc gttcggttta
     3661 cgttccgctt tctggaagtc tttcacggtg atcatgccga tcaggtggaa ttcgtcatca
     3721 accaccagcg ctttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct
     3781 tcaccttcac gcacggtgac cagacgctct ttcggcgtca tgtaaacgct aaccggctgg
     3841 ttcaggtcgg taacaaaacg cacgtcacga ccggtgataa tacccaccag ttcgttttct
     3901 tcggtaacga ccggatagcc cgcaaaaccg ttacgctcgg tcagttcttt cacttcgcgc
     3961 agcgtcgtgg ttggcagaac agtctgcgga tcagtcacca caccagattc gtgttttttc
     4021 acacggcgaa cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata
     4081 ccgccttcct gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg
     4141 gaaagcatag ggatattcag acgaatagtt ttcgtcagct gggtgctgag gtcagcagta
     4201 ttcggcagaa cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct
     4261 ttagcgatac gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc
     4321 attatacaga gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc
     4381 gaagggcggg ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat
     4441 gttaccttct caatcccctg caatttttac cgttagtcgc ctgaatcaaa cggttcgtct
     4501 gctgcttgag catgagatgg gacaggtttg gatcagcggc gaaatctcta atttcacaca
     4561 accggcttcc ggtcactggt actttacact caaagacgac accgcccagg tacgctgcgc
     4621 gatgttccgc aacagcaacc gccgggtgac cttccgtcca cagcatggac aacaagtttt
     4681 agttcgcgcc aatattacgc tctacgagcc gcgcggtgac taccagataa tcgttgagag
     4741 tatgcagccg gccggtgaag ggctgctgca acagaagtac gaacagctca aagcgaagtt
     4801 gcaggctgaa ggtttgttcg atcagcaata caaaaaacca cttccctccc ctgcgcattg
     4861 cgttggtgtg atcacctcaa aaaccggtgc tgcgctacat gatattttgc atgtgttaaa
     4921 acgtcgcgat ccttctctac cggtgatcat ctaccccacc gccgttcagg gcgatgacgc
     4981 accggggcaa attgttcgcg ccattgaact ggcgaatcag cgcaatgagt gcgacgtgtt
     5041 gatcgttggg cgcggcggcg gttcgctgga agatttatgg agttttaacg acgaacgcgt
     5101 agcgcgggcg atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc atgagacgga
     5161 tgtgaccatt gccgattttg ttgccgatct gcgtgcgcca acgccgtctg ccgccgctga
     5221 agtagtgagc cgtaatcagc aagagttact gcgccaggtg caatcgaccc gtcaacggct
     5281 ggagatggcg atggattatt atctcgccaa ccgcacgcgt cgctttacgc agatccatca
     5341 ccgattacag caacagcatc cgcagctccg gctggcacgc cagcaaacca tgcttgagcg
     5401 cctgcaaaag cggatgagct ttgcgctgga aaatcaactt aagcgtaccg ggcaacagca
     5461 gcagcggtta acacagcggc tgaatcagca aaatccacag ccgaagattc atcgcgcgca
     5521 aacgcgcatt cagcaactgg aatatcgttt agcagaaacc ctgcgcgtac agcttagcgc
     5581 cacgcgtgaa cgtttcggta atgcagtaac gcacctcgaa gccgtaagcc cactgtcaac
     5641 gctggcgcgt ggatacagcg ttactactgc tactgacggc aatgtactga aaaaagtgaa
     5701 gcaagttaaa gcgggtgaaa tgctaaccac acgtctggaa gacggctgga tagaaagtga
     5761 agttaaaaac atccagccag taaaaaaatc gcgtaaaaag gtgcattaag ccagcacgaa
     5821 ttcaacgcgt tttttcgata tcaagccatg cccgtgctga cagaagtaat ctactgcacc
     5881 gcaggctttt aacacctgca acggttgatg gcagtctgga caaagagctt tcatttcaat
     5941 aatttccccg caggatgggc aacgcgcatg gccgttatcc tgatcaagca catgctgaca
     6001 ttgtggacaa tgcagttcca tatgaattcc tccggtagcc atccctctac attcatagag
     6061 ggaatggcag ataaaatact tacggataat tatttatttt tcttgatgtg cttcatcaga
     6121 cgcttacgtt tacgcatctg ggttggcgtc agggtgttac gcttgttcgc atacgggttt
     6181 tccccttctt tgaactgaat acgaatcggc gatcccatta cgtccagcga tttgcggaag
     6241 tagttcatca agtagcgctt gtaggaatca ggcaggtctt tcacctgatt accgtgaatc
     6301 accacaatcg gcgggttata accaccagcg tgggcatatt tcagcttcac acgacgaccg
     6361 cgtaccagcg gcggttggtg atcttcaaca gccatcgtca tgatgcgcgt cagcatagag
     6421 gtccccacac gacgggtgga gctgtcatac gcttcacgta ctgattcaaa caagttacca
     6481 acaccactgc cgtgcaaggc agagataaag tgcacacgag caaaatcgat aaagcccaga
     6541 cggaagtcca gcgtttcttt cacctgctct ttcacttcct ggctcaggcc atcccactta
     6601 ttcaccacaa tgacaagtga gcgcccacta ttgagaataa agcccagcag cgagagatcc
     6661 tgatcggaaa taccttcgcg cgcatcaatc actaacatca ccacgttggt atcttcaatg
     6721 gcctgcaacg ttttgattac ggagaatttc tctacagcat cggtgatttt gccgcgttta
     6781 cgtacgccag cggtgtcaat gagcacatac tcacgtccat cgcgttccat tggaatgtag
     6841 atactgtcac gcgtcgtgcc aggcatgtca taaacaacaa cgcgctcttc accaagaata
     6901 cggttagtga gtgtagactt acctacgttc ggacgaccca caatcgccag tttgatcggc
     6961 agactttgcg ggtcgaagtc gtcttcctct tcttcttcgc cgttctcttc cgcttcaaat
     7021 tgcgcccagt attcagcgtc ttcgtcgact tcctcttgcg gtgcgagatc ttccatccac
     7081 ggcagcagca cacgctccag cagacttaat acgccacgac cgtgagatgc ggcgatcggg
     7141 tagatttcac ctaaaccaag cgagtagaaa tcaaccactg cctgatcggg atccagaccg
     7201 tcagttttgt ttgccaccag gaaggtcggt ttttcacgag agcgcagatg tttggcaatc
     7261 gcttcatctg ccggcatcaa gcccgcgcgc gcatccacca taaacagtac gacgtccgct
     7321 tcttcaatcg ccagcagcga ctgttccgcc atgcgggttt ctacaccgtc ttctgtgcca
     7381 tcaatcccgc cggtatcaat acagataaac tcacggcctt caatttccgc acgaccgtac
     7441 ttacggtcac gagtcagacc cgggaaatcc gcaaccagcg catctcgggt gcgagttaga
     7501 cggttaaata acgtggattt tcctacgtta gggcgcccga caagcgcgac cacaggtacc
     7561 atgtttaaag cctcattttt ataaatcatc agacaacgca cgctatattc gcgtcgttgt
     7621 taaaaacagg aaaacggccc ctgtccagga gccgttttca aagtgaacga cagagacgat
     7681 taacgtgtaa tagagtacac ggttccgtct tttgcctgga tcagcagttt gccgtcagcg
     7741 gcaaccggtt cagtctggaa accggaacta tcaacttttt gctgggcaac gaaacgacca
     7801 tcttcgacgt taatccagtg cagataacct tcactgtcac ctaccaccag gttgccatta
     7861 tacagcaccg gcgaagtcag caggcgatgc agcagatcgc tttgtgtcca cagcgtaacg
     7921 ccgccatcaa tggtcaacgc catcacccgg tcattttgat cgaccagata gatgcgattg
     7981 ccgtcgacga tgaaatcatt caccgaaccc agttcgcgtt tccacataat ctgaccactg
     8041 cgcagatcaa gcgccgtcag gttaccatta taggccagag cgaaaacaac gccgttaaca
     8101 acgacgggag tcgtgtcaac atcgctcaga cggtcaattt cggtagaacc ggtcgcctgg
     8161 gaaatacgct gctgccaaat catctggccc tgttccatca gcactgcgct gacgcgacca
     8221 ttatcgcccc ccacgacggc cgcaccaaaa gccgttgccg gcgcagactc gccacgcaaa
     8281 gagagcgaag gcatatcgag gttaactgtc catttgacag cgccgtcagc ttcgttcagc
     8341 gcttgtaact gaccgttact agtgtggatt aacaccagac cgtcgctgac caccgggcga
     8401 gaaagtgctt cacccgcgac tttagtttgc catgccacag taccatcgct ggtattcagc
     8461 gcgtaaacct gcgccttttc gctgccaatg tagacatgcc caccagacac ggtcacaccg
     8521 ccagaaagta atgcaggctc tttagagaac cagccatctt tctcggccag gctgacagac
     8581 cagatttctt tgccatcatc cgcattcagc gcttttacca aaccagcgcg gtccgctgca
     8641 tagacaacgt tgtccgccag tgccggatga agattggaat agaagttgcc aatgccgcta
     8701 ccaacggaag tgctccacgc cgtggtcggc gtaaactggt tttcaacggt tggcaatggg
     8761 gacatcttta ccacatcttc ttcgctgtta aacagcgaac agccgcttaa aagggtaacg
     8821 gaaagcagtc ctggcagcag taatttacgc aattgcatcg ggtccctctc agatggacaa
     8881 attattaatt ttcatctgca tcatttcgct cagtgccgga gtaacatcgc ttttcacgcc
     8941 tgcttcccat gcactacgcg caccttgctt atcacctttg ctcaacaatg ctttaccacg
     9001 caggtcggca acaatggcag cccacccttc acctttgatg gtatcaaggg ttttcagcgc
     9061 ggcatcagcc tgcttgagct gtacctgaac gcgagcaaga cgcagattta tcacggcttt
     9121 gagattttca tcactcgtgt ctgccagccc ctgttgtaac tgggcggcag ctttctctag
     9181 ttcatttttg tcaacaaatt gctgcgcaag ttccaaagaa gccagcgcac cataagtatt
     9241 tttattttca gcagcaaatt tttccgccgc cgggatgcta tccggtttgc cttcgctcac
     9301 tgcggtaacc gcattttgat aggcaagaga agcggagcgt gcagaatcaa cctgatggct
     9361 gttccagtag cgccagccaa tcagtgcgcc aacgcccaaa atcaccccaa cagccagtgc
     9421 tttgccattt tcagcaaaaa agcgtttaac cgcttctacc tggtcgtttt cgttctcgta
     9481 aatttccacg ctgtccttct ccttccttaa cccagtaacg tgcgcaaatg cgcggctacg
     9541 ctatcctgcg caactgccgt ttgctcacca gagcgcaaat ccttcactac tgctgtgccg
     9601 ttagccactt cagactcacc cagcaccaca gcaacgcggg caccccattt atcagcacgg
     9661 gcaaactgtt tcttaaagtt gccgccgccg tggttggtca tcaatttcac gcccggtaat
     9721 tcatcacgca gacgctcagc taatgccata gccgcagatt gtgtatcagc acctgaagcc
     9781 accaggtata tatcgacaac aggatcggct ttaaattccg gattaacggc ctgtactaac
     9841 aatacaagac gttcgaggcc catcgcaaaa ccgactgccg gtgttgcacg accgcccagt
     9901 tgttccacca gaccgtcata acgaccgcct gcacacacgg tgccctggga gccgaggctg
     9961 ttagtcaccc actcgaaaac ggtacggtta tagtaatcca gaccacgcac cagacgctgg
    10021 tttacggtgt aagcgatccc cgcgctctca agcagtttgc acagaccggc aaaatgctca
    10081 cgagattcct cgtccagata atcacctaat gccggagcgt cgttgagaag cgcctgcact
    10141 tccggatttt ttgaatccag cacgcgcagc gggttagtgt acatgcggcg tttgcagtct
    10201 tcgtccagct tttctttatg ctgctcaagg aatgccacca gcgcatcgcg gtaattggcg
    10261 cgtgcttcca gcgaaccaat agagttcagc tcaagagtta cgtgttcgaa gatacccagc
    10321 gcacgccacc agcgggcagt gagcataatc agttcagcgt cgatatccgg accttgcaga
    10381 ccgaaaactt cgcagcccaa ctgatggaac tgacgataac gccctttctg cggacgctcg
    10441 tgacggaaca tcggcccgat ataccacaga cgctgttcct gattgtacag aagaccatgc
    10501 tcgatgccgg cgcgtacaca gcccgccgtc ccttcagggc gcagagtcag gctgtcgcca
    10561 ttgcgatcct caaaggtgta catctctttt tcaaccacgt cggtgacttc accaatcgca
    10621 cgtttgaata gcggggtctg ctctacaatc ggcaagcgga tttcactgta accgtagctg
    10681 ccgagcacgt ttttcagtgt gccttcaatg cgctgccaga tggccgtttc gccaggcagg
    10741 taatcgttca tgccgcgaat ggcttgaatg ttttttgcca cgtttattct ctttctgaat
    10801 ataaaaatga accctcaacg cttccctcaa tgtttcggga gccatgcggg ttcaatcata
    10861 cacgggaagc gaggcgcttc ccatcacgtt attatttttc aacctgctga acgtcaattc
    10921 gacgcgcttc gtccagctga ctggctttcg cacgaatgcg tgcttccagc tggtcgatca
    10981 tatcgttgtt gtccagacgg tctttgcgca cgccatcttc atagaggccg cttttcttgt
    11041 tgccgccggt gacgccgagt gtagaaacca gcgcctcacc tgggccattc accacgcagc
    11101 cgataatcga aacgtccatc ggagtgatga tatcttccag gcgttgctcc agcgcgttaa
    11161 ccgtaccgat aacatcaaat tcctgacgcg aacaggtcgg gcaggcgatg aagttaatcc
    11221 ctcgcgaacg gatacgcagc gatttcaaaa tatcgaaacc gactttgatc tcttcgaccg
    11281 gatcggccgc cagcgatacg cgcagcgtgt cgccgatgcc ttccgacagc agcagaccta
    11341 aaccaatggc ggattttact gccccgctgc gtgcaccacc ggcttcggtg atccccagat
    11401 gcagcggctg atcgatctgt tttgccagca aacgataaga ctcaacagcg aggaagacgt
    11461 cagacgcttt cacgctgact ttgaactgat cgaagttcag gcgatcgaga tgatcaacat
    11521 gacgcatggc ggattccagc aacgcctgcg gcgtcggttc gccatacttt tcttgcagat
    11581 ctttttccag cgatccggcg ttaacgccaa tacggatcgg aatgttttta tcgcgcgcac
    11641 agtcaaccac catgcgaata cgctcttcat taccgatatt gccagggtta atacgcagac
    11701 aatcgacgcc gtattccgct actttcagcg caatgcgata gtcgaagtgg atgtcagcca
    11761 ccagcggcac gttaacccgc tgtttgatga gtttgaacgc ttctgccgcg tccatcgtcg
    11821 gtacggagac acggacgata tcagcgccaa cgcgttccag cgccttgatt tgattgaccg
    11881 ttgcttcgac atctgtcgta cgcgtattgg tcatggactg tacggcgatg ggagcaccat
    11941 cgccaatcgg cacattccca acgtaaatac gtgttgattt tctacgttga attggagcct
    12001 ggttatgcat gaaaaatctc ccgcgttacc cgtctgttac tgcgccggtg attgttcggc
    12061 attgagggtc agacgcgcaa cctggttagt tctgataaaa cgactcagat cgacaggttt
    12121 cccttgatac tggatctgta ctgcggctgg cgcaccaatt ttcagtttat acggtgcctg
    12181 gccggttaaa ttcaaattac cgtctttacg ctgcatcccg ctaaacaatt ttttaccggt
    12241 agcatcagtg acctccagcc agcaatcggc agtaaagttc atcaccagcg cattcggatc
    12301 agccgccggc gtggtcacgc cagcctgatc ggttggcaac ggcgcagcac catctggcgt
    12361 tgttgctgcc gttggtaccg gggtcgcggc ggtatcaaca tttgcctgcg aaggcgaaac
    12421 aaccgcattc tgttgcggat caacagctgg tgctggcgca gttacggcag gtgtttgcgt
    12481 gttggttgcg gtagtatcca cagacgctgg cggcgtggac gttgtagccg ggtctgtagt
    12541 tgtcgacgta tttaacggga cactctgccc ctgctcgcta ttactgctca gttctgccga
    12601 agattgatcg gccatagtgg tgatctcttc ctgctgagct ttgtggtctt gccaccacca
    12661 ggcaccgctc aggccgataa ccacaaacaa caccagccag gtgaaggtca tcagccagcc
    12721 atcacgtttt ttgcggcgtt taccgaggga aaaactctgc attggcgcaa cttttgcagc
    12781 ccgtagtgga gcctgctttt ccagccctgg cagcagttct tcttctggaa tatgtaccag
    12841 acgcgcataa gagcggatat atccgcgcag gaatgttgaa gcaagatcgg cgggtgcctt
    12901 atcttcttca atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg caacggcctg
    12961 ctgactaagt cctagttgtt cgcgagcatt acgcaggcga gcgccggtag taagtgcttc
    13021 attttggtcg tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa tgagaattga
    13081 gatgccggtg aaccatcatg ctcacccaca ccgcgaaaca tccgttaagt taaccgttat
    13141 catacagtat aagaccgtct gtgtgctcgt gacaaagccc acattcacta aggctaactt
    13201 actgttgcat cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta atattgacca
    13261 gacaattgca acttaatact tcattcataa aacattacgc cacggtacat aaagtaaccg
    13321 tggcgtaatg gctatcagac cgctttaatg tcgatggctt caccctgcat ccgtttacgc
    13381 agggtacgtt tcgtacggtc gataacatcg cccgccaact gaccacaggc agcatcaata
    13441 tcatcaccac gagttttacg cacaatagtg gtgaaaccgt agctcatcag cacttttgag
    13501 aaacggtcga tacggctgtt cgagctgcgt ccatacggcg cacccgggaa cgggttccac
    13561 gggatcaggt tgatcttaca cggcgtatct ttcagcagtt ccgccagttg gtgcgcgtgt
    13621 tcagtgccgt cgttaacgtg gtcaagcatc acgtattcaa tagtgactcg gccctgattg
    13681 gcgttggatt tctccagata acggcgcacc gcagcaagga acgtttcgat attgtacttt
    13741 ttgttgatcg gcacaatttc gtcacgaatt tcgtcgttcg gcgcgtgcag ggaaattgcc
    13801 agtgcaacgt cgatcatatc gcccagttta tccagcgccg gaactacacc ggaagtagaa
    13861 agcgtgacgc gacgtttaga caggccaaaa ccgaaatcat caagcatgat ttccatcgcc
    13921 ggaacgacgt tgttcaggtt gagcagcggc tcgcccatgc ccatcatcac tacgttagtg
    13981 attggccgct gaccggtgac ttttgctgcg ccgacgattt tcgccgcccg ccacacctgg
    14041 ccgataattt ccgacacccg caggttgcgg ttaaagccct gctgggcggt ggaacagaat
    14101 ttacactcca gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg gtcgtcttcc
    14161 gggatataca ccgtttcgac gcgctgatcg ccaacggcga tcgcccattt aatggtgccg
    14221 tcggatgaac gctgttcttc aaccacttcc ggtgcgcgga tctccgccac ctctttcagt
    14281 ttgccgcgca acactttgtt gatgtcggtc atctcatcaa agttgtcgca gcaatagtga
    14341 tacatccact tcatcacctg atcggcgcgg aagggttttt cacctaaatc tttaaaaaac
    14401 tcccgcatct gctgacggtt gagatccagc aggttgattt ttccatcttt cgtggtgacg
    14461 ttttcaggtg tgactaattg ttcagacata tgctattccg gcctcgttat tacacgttat
    14521 ggcccctgga gggttgaaaa aagaaacgcc ccggtgagcg gctgctcgtc cgggggcgct
    14581 gcattgtaca aattctggcg cacggatgcc acgtttgcac gcggcattta cgaaattatt
    14641 aacgggtgcg cgggcacact tcgccttcgc cgaagaaata agcgatttcg cgagcggcag
    14701 attcgacgga atcagaaccg tgggtaccgt tttcggtcag gctgtcagcg taatcagcgc
    14761 gcagagtacc ggccagtgcg tttgccggat tggtcgcgcc cagcagatcg cggtgacgct
    14821 gaacggcgtt ttcaccttcc agcacggaaa ccacgatcgg gccagaggtc atgaattcaa
    14881 ccagaccatc aaagaacggt tttccatcgt gttcagcata aaagccacga gcctgttcaa
    14941 cggtcaggtg cagcattttg gtgccgacaa ttttgaaccc tgcagcttca aagcgcgcaa
    15001 agatattacc aatgacgttt tttgctaccg cgttcggttt gatgatggaa aaagtacgtt
    15061 caatagccat ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc gtacgagatg
    15121 gcgcggatta taatgagcaa cagggccgtt gactattgat gaaggtaaca tttttttaaa
    15181 ataaaatgag ttttagcaac aatcagggac aaaacagact attgcatgac aaatttcact
    15241 gtcgcgattt gccccacgtc atccatcacc agcaattgat aatcaccttt atccgtcaaa
    15301 tgcagggtca cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt
    15361 tcacctgccc ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt
    15421 ttaataatcg cgccatcgcg aacgcctgtc agctgcagag ggagctgggc atcgtgaccg
    15481 tatggcgggc aaattgtcga ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc
    15541 agccagggtt ccagcggtag cggccagaca tttaccattt cttgcttcgc ctgcgggcag
    15601 tcagcggcga cgcgctgccc tttgtcatcc agccagatgg ggaagcgaat gccattaatc
    15661 ccttcctgct ctgacagtaa cagcgttggc ggctggctgc catccagcag ccaggttgcc
    15721 aggcggcggc gacagttacc gtcaccttcc ggcaaagact gtccgcccgg ccagcagata
    15781 acgccacgag tgactgagtt cgggcgcggg tcttccggca gattcgcact gcgcgacagt
    15841 aagatattat tgacctgatt caacaatggt acggcactgg caaagccaaa ctgaccaaca
    15901 acgggcgtgc cgtcaggtct gccagtccag atcccaatga catagcgagc gttaacccca
    15961 atcgcccagg cgtcacgata gccatagctg gtgcccgttt tccatgccag tggggcgacg
    16021 cgcggcaagg cactatccgg caagggttgc gcttcatcag ccataatccg gcgaatgatc
    16081 cacgccgccc ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc
    16141 aatttgcctg ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat atcttccagt
    16201 tttgcaccag cgccgccgag aatgagtgaa agattcggcg cagcgccgtt gggcaaatat
    16261 aacggcaatc caacattgcg taactttgcc gcaaaccgtt tcggtccata ggcttccagc
    16321 acctgcacag caggtaagtt cagcgagcgc accagcgcct cgctcatgct gatcgggcca
    16381 tgaaaaccgc tatcaaagtt acctggtcga taatcaccgg tgcgccgggg gacgtcttgc
    16441 agcagtgatg ccgggtggat caagccttca tccagcgcca gaccataaac aaacggtttg
    16501 agcaccgatc ctggcgatcg gatcgcattg accatatcaa catgaccaaa gcgtgaatca
    16561 tcgttgagat caaccgatcc cacccagccg cgaacacgca tatcggtatg atcaaccacg
    16621 atcatcgcca gtgagctgcg cggtggcaac cgccctttcc agttttgcgc cagttcttcc
    16681 agacgtcgtt gaagaccggc atccagcgta gtaacgattt tgtcgctttt gcttttaccg
    16741 agcatcatgc gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc cagccagatg
    16801 ggttcttccc ttgactcttt tacctgctca cgggaccaca caccttgcgc agccatccgt
    16861 tcgagcactt tattacgcgc ggcttcggca cgctccggcc aacgatccgg gcgaagacgg
    16921 ctgggcgctt gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa attcgcaggc
    16981 gactttccga gataagccca acttgccgca ccgatcccct gcaacgtacc gccaaacgga
    17041 gcgcggttaa gatacaaggt cagaatttca cgcttagaca gatgccattc cagttgcaac
    17101 gcgcgccaga gctggcgaat tttgccgcca aatgttttgg ggtgaggatc aagcagacga
    17161 gcaacctgca tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt gagatcttgc
    17221 catgctgcgc gcgccaccga gaatggattc acccccggat gcttccagaa ccagcgatct
    17281 tcataattga tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt taccggataa
    17341 cgccagatgc cgtcagcatc ggcgaagcgc cagagcggcg taccatcctg cgccacgacc
    17401 actcgtgcgg gattgacttc atgcagcggt agaggccaga gtttatctgc tccccacgct
    17461 gcaagaaaaa ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg tttggttaac
    17521 aagcgaggca tttacggtcg gacaatcagc agatcttcag ccgcgccggt cgcccgccat
    17581 tggggaacat acattgattc caccatcggt tgcggtacct gatacgttcc cggcgtcacc
    17641 gcccgcgcca gatacaccag cgttaccggt tggtattcat caacggcaac cgccgccaca
    17701 aagcgatcgt cacggaactc aatgtgctta atactcgcct gctgcatctg gttcagtaag
    17761 ttctgcactt cgccaccact ttgctccagg ctggcgctac cgttcgccag attctggttt
    17821 tccagttcca gacccacagg cagcagatcc acgactaacg catccggcac gctgttactg
    17881 gcttttacct gcaaccacac cagcaccaga tcgccgctac gtaacgagtc cagcgatttg
    17941 ctcttaccat cagtaccaag aatatgacgc tcgatttgca gcacattgtt cgccggtaaa
    18001 ggtgcggatt gcggataacc gctggcatcc atacgcaacc ataacggctg atcgccactg
    18061 ttgctcacct gcaaggtgac aagttgatcg ctattcagat tgctgttttg cgctttctcg
    18121 cctgtcagcg gctcagctga gaaagaggtt tgcgcctgcc atttacccgg taaatcctga
    18181 atcgtacggg cagccaggaa caacgcgtta ctttcctgcg tcgatagcca gcgttcacca
    18241 aacgcctgct gcgaaagtgt gttcagcaaa gtgtactgct catcgggtag cagtttattt
    18301 tcttccagca aggagagcat taacgcgttg tcgcgcagtg gactaccgta atcacccagc
    18361 catatccgct catcactatt tctcggcgtt ttcagcgcca gcgcaatcgc ttcttcacca
    18421 cgcgtcgcat cacccatggt tttcagcgca atgccaagtt gcagcagcgg taaaccagaa
    18481 gcggcatctg cacgatgctc ccagatttca cgcagcgcac ccagtggtgc tttttgctga
    18541 cgggccagca ccagcgcagc gtaagactga acggcgaatt tactggcttt gagattatcc
    18601 gcgtacggga tcgacatcat gcccggatct tgtaaatagc gcagcagacg ctcattaccc
    18661 cggttaatgg cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac caggaaatcc
    18721 atcacgtaag ccgtcagcca gtactcttcg tcaccgtttt tatcccacag cgcaaagccg
    18781 ccgttatcac gttgcatttg cagcaaacgg gaaatgccga tatcgaccga tgcacggcgt
    18841 ttctcatcac tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt ggtataaagt
    18901 gacgggaaca ggccgctggc ggtttgctca agacagccgt acggatacgc ttttaactct
    18961 ttgatataac gtgcaatgtt cagcggtggt ttgccgctca acaacaattg cccttccagc
    19021 gtaacaggcg agaagttttg caatccatcc gccggaatcg cccatgtctc accaggctgt
    19081 aacgccgtac cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc gattttccac
    19141 tgcttatgct gatcggcaac ggtttcaccc ggtaacgcta acccgctaat ggtggcctga
    19201 atttctccat cgccataacc cggcaatgct cgcaccggga taaacagcgt agtacgcaca
    19261 cctggcgcta attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa accactggcg
    19321 gtcagggcaa cgttcagttt ttgcggttta tcggtaagat tagtgatatc cagcgtcaga
    19381 cgcgaggtat cgccactcgc cataaagcgc ggcatgttca gttcagcaat caccggtgcg
    19441 gcaactatca ctttgctttc gttgctaccg aaatcatctg ccgtccaggc ttgcgccatg
    19501 acgcgcaatt caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc gccctgttcg
    19561 ttgagcgtta ccgacagcgc ctgctgcgcg acaatattga cgtgattgac cggcggttta
    19621 ccaccacgtt tcagctcatc accatcgcca ccgaaacgca gagctgccag acgcccctga
    19681 ccttcaataa cctgaccgta aatatcgtaa atgtctgcgc catagcgttt ctgaccaaag
    19741 aacgcctgcc acggatctgg cgtaacgtag tcagtaatat tcagcacacc actatcaacg
    19801 gcagacacca gcacattcac ctgtttaggc ttctcgccat ttttagtgct ggctttaatt
    19861 ttcacggtta atggttgatt gggacgcatt tttgctggtg tttccagcgc cagatcgagg
    19921 cgacggtttt catcaccaag cggcagatgc aacacaccaa ccgcgcgttt tggcgtcgcg
    19981 gagcgagatt tatcgccagg acgcaccacc agcgtactca aataaagatc atgacgattc
    20041 caagttttat cgaccggaat cgtcagatcc agcccttgag ccggaacatc aatctcttgc
    20101 caccacagcg gcccttcact ggactcgacc atcgcataac ctttacccgc cgttggcgcg
    20161 gcaatatgca acttaatggt atcgccgggg cgataactgg ctttatctag tttcagcgtg
    20221 acacggtcgg gtcgcactgc gccgctcccg tcgctgttgt cctgccagct atagccagcc
    20281 caaaaacgca cgctactgac cgcttcattc ggcgctttga cttccagacg ataagcgccc
    20341 cactctaccg ggaaactgac cttaccggtt tcatccgcct gaagatccag agtttgttca
    20401 ttttcgatca gatctttttg atcaaactgt gactgccagc cttcatcttc tgaccagttc
    20461 cagtagtaat cgcggcgttc gcgaatcagg cgcacctgca agcccgacac ggcttttttc
    20521 acgccttgcg catcgctata aacgatgtca aaagcggcgt tactgccttc atcaacaatg
    20581 ggctgtttca ccgtgctgtc agtgcgataa tcgtaaaccg atttcaaggc gaactgcgga
    20641 cggagccccg gcaatgcatc ggcaggccag atagcctgct cagcgcggcg cgtcaccggg
    20701 cgaccgcccg attccagcaa actgccctgg aaaataacct gcaatgggga atgcgtttcc
    20761 ttccactggc tttctgtaga aacttcgccg cgccctttat catccagcgt caactgaact
    20821 tcatccagcg tgcgggaaag gttttcggca gctatatcgc cgaattcaaa accaggtaag
    20881 gctgacacag cctcacgcag tgggcgcagg aaaagttgcc cttgcaaagt attaccatta
    20941 gcaggcgcac cgtacaggta gtatcccacc acggagaatt tcacttcatc attcggcgta
    21001 agcggggttt tctcaccggt cagattcagc gccatacgct ctggcataaa atcttcgacg
    21061 tggaaatccc acatccggta ctggttatcg cccgtgttgg cgcgaatatg ccacataccg
    21121 gttgccgcat tgctatcgag tggccaggta aagtggtaga ggccattctc cggctgacta
    21181 acgacgttcc tgagtacctg cccgtcaggt ttaatcacgt ctaacttgat gggttgatcg
    21241 ggcaatgctt taccgtctgc atcgcgcagt aaaccattga ggattaccgt ttcacctggg
    21301 cgataaagat cgcgtgggcc aaacatgaaa aactgtttgc tatagcctgg cgcgccagca
    21361 atgttaaatt ctgctaagtc cagcgccgga agttttaaat cgagtagcgt tgtctgaccg
    21421 tctttacgtg ccagcagtaa tgccgcattt ttatcatttt ccagctgcac atgcccctga
    21481 gcgtcactgg ttgcctgagt cagagtctgt cctttctcat ttaataaaga gacttcaatt
    21541 ccttgctggg ccgcgccgtt ttccagactt tgggtaaaga tatccagacg attgtgataa
    21601 cggtgagctg aaacgccgat atcacttaac gtaaacagcg tcgcgggatt actgtaatcg
    21661 taacgtccag cctgattcat cacagccaga tacacgcccg cctgctgaag cggtttgata
    21721 tcgcccagcg gcagcaataa tttttcacgg gtgttacgcg caggattgag atcaaaccgt
    21781 ccggtgtaga ccagatccgc catctgcagc agtttgtctg actgccagtt cgccagcgaa
    21841 ttgcggtatt cccattggct aatgaatgct ggcagagatt ctggcttaac gcggaagaag
    21901 ttaacatcga cattattaac gttgagcgcc attaccggca gcccttcaac gactttgcca
    21961 ggcagcagcg aaccacggct ggcaaaaccg acgctgggtt ggatgtcgcg ggtagttata
    22021 gttttttcgt aatctttact gaaggttgcg ttgttgagtg ctttgacctc cttgccaata
    22081 gtaacgatca aatcacgttt cggttcgagg tggcgtaaac gtagctcttt aagattatct
    22141 gacagctccc aggcaccatc cactttgccg ctttttttat cgacgacatg aataacgcgt
    22201 gagaaatcct gatccgggtc gagagggatg gagaacgtca gcaccagcgt ggctgcacca
    22261 tcaagttgga cttcagagag atccagcaat gtcagcacct taccggcact ctgttgggcc
    22321 agtttttgtc tctccggcac ggagagtttt gctgaactcg cgttttctga agaggccgct
    22381 ttagtaactt cagaaggcgc atcttttttc accgctgttg gcgcgttatc gttgttgtcg
    22441 caccctgcca gcgccagcat tagcatgcag gcggctacgc gtaacttttt catttttcat
    22501 ccctgccaca atggcccgtt agcaacgtcg aataattatt atgcgtgaga atttacgtta
    22561 tgtaattcag tatcacgccc atacaatccg aattttagtg agaattgtgg ctcagacagc
    22621 caaatcagcc tctttcgctt gtcgcttttc actaaacatc cgacaattta gcctaacccc
    22681 ggcaaaaatg gagatgccta tgtccacgac atggtttgta ggagccgact ggctcgccga
    22741 acatattgat gacccggaaa ttcagattat cgatgcccgc atggcgtcgc ctggacagga
    22801 agatcgtaac gttgctcagg agtatctgaa tggacatatt cccggcgcag tgttttttga
    22861 tatcgaagcg ctttctgatc acacttcccc gcttccgcac atgctgccgc gcccggaaac
    22921 gttcgccgtg gcgatgcgtg aattaggcgt taaccaggat aagcacctga ttgtctatga
    22981 cgaaggtaat cttttctcag ccccacgagc atggtggatg ctgcgcacct ttggtgtaga
    23041 gaaagtgtcg attctggggg gtggacttgc aggctggcag cgcgatgatc tgctgttaga
    23101 agaaggtgca gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc ctgaagccgt
    23161 ggtgaaagta accgatgtat tattggcaag ccatgaaaat acggcgcaaa ttattgatgc
    23221 ccgcccggct gcacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg
    23281 acatattccc ggagcactga atgttccgtg gacggaactg gtgcgcgaag gcgaactaaa
    23341 aacgaccgat gaactggatg cgatattttt tggtcgcggc gtcagctacg acaaaccaat
    23401 tatcgtcagc tgcggctctg gtgtaacggc agccgtggtt ttgttagcac tcgcgacgct
    23461 ggatgtgcca aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cgcgggcaga
    23521 tttaccggtt gagccagtga aataagtatt ttacaggcaa taaaaaaccg ccgaatttgg
    23581 cggtttttta ttgctggtct ggttcgcggc ctttccagca ggttgtatta ccgtagtaat
    23641 gcaagcgcgt ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga
    23701 gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc
    23761 gggcgctggc ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt ctcagcgctt
    23821 gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca
    23881 atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt ttcccaagta
    23941 aataataacc ggagtctctc cggcgacaat ttactggtgg ttaacaacct tcagagcagc
    24001 aagtaagccc gaatgccgcc ctttagggcg gcatatttta gattatccgg ttttgtttaa
    24061 agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cacaatatgt tcggtaataa
    24121 agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt tcatcgccag
    24181 gtaatgtatc ggtcgctaca cttcccgtcg cctgaataat ttcttcgata tcaccatcgg
    24241 cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa
    24301 aagcacgctt caccggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg
    24361 ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc
    24421 tgctcagcgg atttccctct tcaccaatca acaaactgat ttcacgcggc ataaattctt
    24481 taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc tcaaacaatg
    24541 tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agcgcttcta
    24601 acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa
    24661 gcgcactatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg
    24721 attccagtag agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct gctttttcca
    24781 gcaggtcttc aagttcgttt tttgtttcgg acataagaac cacaattcat tcaacgttcg
    24841 gggcgcaaat gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga
    24901 atttgcagaa tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg
    24961 ccgttaacag attagctatc gtgcgcacac caagtcccgt agcgcccgca gaccactgtt
    25021 caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt
    25081 tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc
    25141 ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca
    25201 gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagcaaa cgaccggcaa
    25261 gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc
    25321 cggtgagggt cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac
    25381 catcggcaag caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt
    25441 tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac
    25501 agcagaggaa cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat gccagcgccc
    25561 cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt
    25621 tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacactg
    25681 gcgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg
    25741 aaccgcgtcc gacggtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg
    25801 tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg
    25861 ccagttgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga
    25921 tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca
    25981 ctttacgcgt gcctttcggg gctttgtaac cttgccagaa tgcccagcag cgatccgcat
    26041 cccagccttc accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgcgccg
    26101 cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat
    26161 taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa
    26221 tcttcatcgc ttctgtcatt ttagttatcc ttctttgtaa aagggccgcc agttggcagc
    26281 cccaggattt ttccgctaaa tgattcgcgt tgcagaaagg cggcaagtga gtgaagcccc
    26341 aggagcatag ataactatgt gactggggtg aacgagcgca gccaacgcat ctgcggcgtg
    26401 aagcatgacg cggaaattat tcggcctcgt ccagccagac taacaaaatc gcttcgagga
    26461 ttttctcgtt ggatgcctgc gggtcgtcgt cgaaatcttc cagatcgcaa atccactgat
    26521 gcatatcggt gaatcgaacc gttttcggat caagatcagg atacgcatcg tacagtgctt
    26581 cgccaatttc gcggctatcg gtccacttaa gtcccatact aacctctgtt aatgctcacg
    26641 cgcatggttg atagtgtaac gcgggatttc gactactaaa tcttcgtcgg taacgcgtgc
    26701 ctggcagctt aaacggcttt cgggctccag tccccaggct ttgtccagca tgtcgtcttc
    26761 ctgctctgag ctttctggca gcgagtcaaa accttcacga acgatgcagt ggcaggtggt
    26821 gcaagcacag gatttttcac aggcgtgttc aatctcgata ccgttacgca gagctgcgtc
    26881 gagaatggtt tcaccgctat tagcttccag aacagcgcca tcaggacaga gatcctgatg
    26941 aggcaaaata acaatctttg gcatattaaa cctcgtccac ggaatggcct ttcagcgcac
    27001 gacgaaccga ctggtccatg cggcgagcgg cgaaatcctg ggtttgtttg tctacgtttt
    27061 taatcgcttg ttcgatggcg tcaacgtcat cgccctgcgc cacttcactc aggtgagcgg
    27121 cagcatcgtc aatgacctga cgttctgcgg cgcttaacag cgcggcatca gcagccagcg
    27181 cgccgtgcag actttccagc acacgcgccg cttctacttt ttgttctgcc agcattcggg
    27241 cttttacgtc ctgctcggca tagctcattg agtctttgat catcgaagcg atttcgctat
    27301 cggtcagacc gtaagacggt ttgacctgaa tagacgcctc aacgccggtg gatttctcca
    27361 tcgccgtcac gctcaaaaga ccgtcggcat cgacctggaa cgtcacgcga atatgcgcac
    27421 caccagccgg tagcgccgga ataccacgca gcgcaaaacg cgccagtgag cggcagtcct
    27481 gcaccagttc gcgctcaccc tgcattacat ggatagacat cgccgtctga ccatctttaa
    27541 aggtggtgaa atcctgagcg cgggccaccg gaatagtggt attacgcgga atcactttct
    27601 ccaccaggcc gcccatcgtt tcgaggccca gcgacagtgg gatcacatca agcagcagca
    27661 tttcgctgtc tggcttgtta cccaccagaa tatccgcctg aatcgccgcg ccaatagcga
    27721 cgactttatc cgggtcgatg gaagtcagcg gtggacgacc gaaaaattcg cctacccgtt
    27781 cacgcaccag cggcacgcga gtagaaccgc ccaccatcac cacttccagc acttcgtcag
    27841 cttctacacc cgcgtcttta agcgcgcgac gacaagccag taaggttcgt tttaccagtg
    27901 gcgcgatcag ttcattgaat tgttcacggc tgatttcgcc ctgccagccc gcaacgttaa
    27961 cggtcacgga gtccgcatcg ctcagcgcga ttttggctgc aatggcggca tccagcagtt
    28021 cacgctgaac gcggttatcg ctacgatcag gaatgcccgc ctgctcgcga atgtaatccg
    28081 ccagcagatg gtcgaaatca tcgccgccga gcgcggaatc accgccggtt gccagcactt
    28141 caaacacgcc gcgacttaag cgcagaatgg aaatatcaaa cgtcccgcca ccgaggtcat
    28201 aaacagcgat cacgccttcc tgaccggaat ccagcccgta ggcgatagcc gcagcggtcg
    28261 gttcgttaag taagcgcaga acatgcaacc ccgccagacg cgccgcgtct ttggtgccct
    28321 gacgctgggc atcgtcaaag tacgccggaa cggtgataac tacaccatcc agctcgcctg
    28381 ccagggcttc agttgcccgc gccgccagtg ctttgaggat gtccgcagaa acgcgcaccg
    28441 ggttcagcag ccccgccgcc gtttcaatca tcggcaggcc gttttcgctg gcctggaatt
    28501 gataaggcag atgcggatag cgttgctgga tatcagccag cgagcgcccc atcaggcgtt
    28561 taacagaact aatggtgttg gcggtgtcaa gcgctgcgtt agtacgcgcg tcataaccca
    28621 ccgaatgccc ttgctgttga tagtgaacaa cagatggcag caggtgacgg ccttcatggt
    28681 cggctaacgt ttcggcctga ccgctgcgca ctgtcgccac cagcgagttg gttgtgccca
    28741 ggtcaatacc agccgccaga cgacgctgat gcggcgcagc actcaaacca ggttcactaa
    28801 tttgtaataa ggccatgttt agcttccaga aattaaaaat cgagcagttt ttcttcgagt
    28861 tgttcggcac tgctccgcag tttatcgaga aaacgcagct tacgcacggt atccgccgcc
    28921 gcgtcccacg cctcgttgtc taactgttca accatcaact gatggcgggt atcaaacatc
    28981 tttttcacac gtttgataaa gctttccagc cgcgcttcat ctttcgcctg ttcgatctcg
    29041 tccagctctt cgcgcagctc caactgttcc atcaggaacg cggtgtcgcg cacagtatgc
    29101 tgctcgctgg cgagatcaaa accgtgtaaa gaaagcaaat attccgcgcg cattaacgga
    29161 tgacgcagcg tttgccaggc ctggttaatg gttgcagatt gctgtacggc ggcgagttgt
    29221 tccgcctggc ttccgctggc gaatttatca ggatgatact gacgttgtag atcctgaaaa
    29281 cgcaggctca gcgcctgggt atcgagttga taacgggcag gcaagccaaa gaaggtgaag
    29341 taatccataa cattctcagg gctgcgatcc gccgcgataa cggcgaatca ggtaaaacaa
    29401 accccacgcg caggcgacca cggtggggtt atcggtatgc gcatcaaacg tggaagcttt
    29461 cgccgcaacc acactcatct ttgacgttcg ggttggtgaa tttaaaccct tcgttcaggc
    29521 cttcttttac gaagtccagc tgcgtaccgt ccagaaattg caggcttttg ccatcgacca
    29581 cgactttcac gcctttgtct tcaaacacga tgtcttccgg cgtcggttcg tcaacaaatt
    29641 ccagtacata agccatacct gaacatccgg aggttctcac gcccagacgc aggccaaacc
    29701 ctttgccgcg gttagccaga aaggtattta ctcgcgctgc tgcactgtcg ctcagtgtaa
    29761 tcgacataac caaacctcaa ctcttatttt gcttcacgtt tgcttttata gtccgcaatg
    29821 gcggctttga tcgcgtcttc tgccagaata gaacagtgaa ttttcaccgg cggcagttca
    29881 agttcttcag caatatcggt atttttgatc gcctgcgctt cgtcgagaga cttccctttc
    29941 acccattcgg tgaccaggga gctggaagcg atagcagaac cgcagccgta agttttaaaa
    30001 cgcgcgtctt caatgatacc ttcatcgttg actttaatct gcaacttcat cacgtcgcca
    30061 caggccggtg cccccaccat gccgctgccg acgttctcgt cgttgttgtc aaaggaaccc
    30121 acgttacgcg gattctcgta atggtcgata actttttcgc tgtaagccat tataaattct
    30181 cctgattccg ataccgatta atgatgagcc cattcgatgc tgttcagatc cacgccctgc
    30241 ttgtacattt cccacagcgg agaaaggtca cgcagacgac cgatggattt acgaactaac
    30301 tcgatggtgt agtcgatctc ttcttcagta gtaaaacgac ctaaagagaa acggatagag
    30361 ctatgtgcca gctcgtcgtt cagccccagc gcgcgcagca cgtaggacgg ttcgaggctt
    30421 gctgacgtac aggcggaacc tgaagaaact gcgaggtctt tcagcgccat aatcagcgac
    30481 tcaccttcaa cgtagttgaa gctgacgttg agaatgttcg gcgcaccgtg ttccaggtca
    30541 ccgttcaggt aaacttcttc gatatctttg atgccgttcc acagacggtt acgcaggccg
    30601 cgcagacgtt ccatctcggt cgccatctct tcttttgcga tgcgataggc ctcgcccatt
    30661 ccgacgatct ggtgaacagg cagagtgccg gaacgcatac cgcgctcgtg accgccgccg
    30721 tgcatttgcg cttcgatgcg tacgcgcggt ttacgacgta catacagcgc accgatacct
    30781 ttcgggccat agattttgtg accggagaaa gacatcaggt caactttcaa ctggctcagg
    30841 tcgataggca gtttgcccac gctctgggtt gcatcaacgt gatagataat gccacgagca
    30901 cggcacattt cgccgatagc cgcgatatcc tgcaccacgc cgatttcgtt atttacgtgc
    30961 atgatggaaa caaggatggt gtcatcacgc atcgctgctt caagttcttt cagatcgata
    31021 atgccgttac gctgcggtgc caggtaggtg acttcaaaac cttcgcgctc cagctgacgg
    31081 caggtatcca gtaccgcttt gtgttcggtt ttgctggtga tgatgtgctt gccttttttc
    31141 tgataaaagt tggctgcacc tttgatcgcc aggttgtcag attcggttgc accagaggta
    31201 aagacgattt cacgcggatc agcgccgacc agatcggcaa tctgattacg ggcgatatct
    31261 accgcttctt cagcctgcca gccgaaacgg tgagaacggg aggccgggtt accaaaggtt
    31321 ccgtccatcg tcataaactg catcattttc tcggcaacac gcgggtccac cggcgtggtt
    31381 gcggagtagt cgagataaat cggtaatttc attgctctat aaactccgta catcactcaa
    31441 tgcaaggaac caggctaccg gctggatgta cgaccgtgtt tacggagcgc gatacgcccc
    31501 ggcctgattc taatatcttg ttatctttaa cctaactgtt tcgggtttca gcaagttgac
    31561 aaacttacga atctccggga gcttacataa gtaagtgacc ggagtgagta tgtgcagtca
    31621 acgcagatgc aacacgaaac aggacggtta aattaagcgc gtaacttaac gtcgatcgcg
    31681 tcttgtatgc gggtgcgtgg cgcgtcgtga gtatgctgac gaccagacac atccagcact
    31741 tcctggttat taaccagttc gcctaaagta atgttgttga gaaaaccggt gagacggtcg
    31801 ctcaaatcac gccacagcgc gtgggtcagg catttatcgc cgccctggca accgccttta
    31861 ccctgacaac gggtagcatc tacagattcg tcaacggcgc taatcacttc gccaacggcg
    31921 atgctgctgg catctttgcc taacagataa ccaccgcctg gtccacgtac gctggaaacc
    31981 agaccatttt tacgcagacg ggaaaacagt tgttccagat aagaaaggga aattccctga
    32041 cgttcggaaa tatcagccaa cggtaccggg cccgcttcag agttgagcgc aacgtcaagc
    32101 attgcggtca cggcatagcg ccctttagat gtcagtctca tgtcttactt cacctcaaac
    32161 tcgcccctgc ccggggtttt ttattgtaaa agtgggggta ttgcatagca gggtcaagtc
    32221 tgacattccc gagtaaattg gtcaactatt tacttgactg atttagtcgg gtatttaacc
    32281 ttcagtgcca tttttttatc gtggcgtttg tacctgttgt ccttccagac catgggcggg
    32341 ttgagggcaa ttattcgcat tagcgctggc ctcttcccct caccctaacc ctctccccga
    32401 aggggcgagg ggaccgtccg tgcatttatc tggcttagcc ctcaggcctt attaccttta
    32461 ttctgctgct ctatagaagc cagaatcccg cgcaggatat tcaattcctg gctttccggg
    32521 cgcgcacggg taaacagacg gcgcaattta ttcatcacct gccccggatg gttttcacgg
    32581 ataaaaccgg ttgccagcag cgtttgttcc agatgaccat aaaaacgctc cagatcatcg
    32641 accagcggat acggcgtctc ttcatgctcg acctgctcgc cattttcctg agttgccagc
    32701 caggccatac gcacttcata ggcaataacc tgaaccgcca ttgccaagtt cagcgaactg
    32761 tattccgggt ttgccgcaat cgcgacatga taatggcatt tctgcaactc ttcattggtc
    32821 aagccgacgc gctcacgacc aaacaccagc gccaccgggg tatttgccgc ttcagcgacg
    32881 cttttcagac cgcattcgcg cgggtcgagc atcggccacg gcagcgtgcg ggagcgtgcg
    32941 ctggtgccca ccaccaggct acaaccggct aacgcttcgt cgagcgtatc gacgatgtga
    33001 gcattaccaa tcacatcgct ggcccctgct gccagggcaa tcgcctggga gtcgggtttc
    33061 accagtggat taaccagcca cagattggtt aatcccattg ttttcatggc acgggcaaca
    33121 gaacccatat tgccggtgtg tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt
    33181 gtctttcttc atctaaagat tattcacgca tcttatcata aaacgaagac agatgccgat
    33241 ctcgctgcta tactctgcgc cgttttcccg ttctttaaca tccagtgaga gagaccgatg
    33301 catccgatgc tgaacatcgc cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa
    33361 aactatgaaa ccccggacgc tgtagaagcg agccagaaag gcagtaacga tttcgtgacc
    33421 aacgtagata aagctgccga agcggtgatt atcgacacga ttcgtaaatc ttactcacag
    33481 cacaccatca tcaccgaaga aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg
    33541 gttatcgatc cactggatgg cactaccaac tttatcaaac gtctgccgca cttcgcggta
    33601 tctatcgccg ttcgtatcaa aggccgcacc gaagttgctg tggtatacga tcctatgcgt
    33661 aacgaactgt tcaccgccac tcgcggtcag ggcgcacagc tgaacggcta ccgtctgcgc
    33721 ggtagcaccg ctcgcgatct cgacggtacc attctggcga ccggcttccc gttcaaagca
    33781 aaacagtacg ccactaccta catcaacatc gtcggcaaac tgttcaacga atgtgcagac
    33841 ttccgtcgta ccggttctgc ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac
    33901 ggtttctttg aaatcggtct gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt
    33961 gaagcaggcg gcatcgtcag cgacttcacc ggtggtcata actacatgct gaccggtaac
    34021 atcgttgctg gtaacccgcg cgttgtgaaa gccatgctgg cgaacatgcg tgacgagtta
    34081 agcgacgctc tgaagcgtta atgactcagg cgggtgatgt cactcacccg ccctcgcctt
    34141 tcaggcgcta ttctgaaata cttcctcacc gctttacttt ctttcgtcac tctcccacca
    34201 tctttccccg attaatggat gataaagaag tcacaaggaa aggttatggc gctgccagtg
    34261 aacaaacgcg ttctcaaaat tctgtttatt ctctttgttg ttgccttctg cgtttattta
    34321 gtgccgcgcg ttgccatcaa cttcttctat tatcctgacg ataaaattta cggtcccgac
    34381 ccctggtcgg cggaatccgt cgaatttacg gctaaggacg gtactcgtct gcaaggctgg
    34441 tttattcctt cttcgacggg ccctgctgac aatgccatcg caaccatcat tcatgctcac
    34501 ggcaatgccg gaaatatgtc cgcccactgg ccgctggtca gttggttacc cgagcgtaat
    34561 ttcaacgttt ttatgtttga ttatcgcggg tttggtaaat caaaaggcac gccgtcccag
    34621 gccggattgc tggacgatac gcaaagtgcc atcaatgtgg tgcgccatcg cagtgatgta
    34681 aacccacaac ggctggtgct gttcgggcag agtattggcg gggcgaatat tcttgctgtt
    34741 attggtcagg atgatcgtga aggcatacgt gcggtgatcc tcgactccac atttgcctct
    34801 tatgcaacca tcgccaatca gatgatcccc ggcagtggct acttacttga tgagagttac
    34861 agcggcgaaa attatatcgc cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa
    34921 gctgatcacg ttatcccatg gcagcacagc gaaaagttgt atagcctggc aaaagagaca
    34981 aaacggttga tcctaatccc ggatggcgaa cacattgatg ctttttccga tcgtcacggc
    35041 gatgtttatc gcgaacagat ggtggacttt atccttagcg cgttgaatcc gcagaactaa
    35101 cccatgatcg ctagcacgat aatcattcac aaaaccacct taagacatgc taatccactg
    35161 gtcagaacag tttaagatga gaaaaattct gtgacgcttg ccaacatttc tgatgattag
    35221 cattcccttc gccatctccc tgagcaaact ttagctattc ttatcaatta tgcttatggg
    35281 agatctacag atgatgccta cgcttgctcc accatctgtc ctttcggctc cccagcgccg
    35341 ctgccagatc ttgctgacgc tctttcagcc ggggttgacc gccaccatgg caaccttcag
    35401 cgagcttaat ggtgtggatg atgatattgc cagtcttgat atcagcgaaa caggacggga
    35461 gatcctgcgc tatcatcaac tcacactgac gactggttat gacggtagct accgggttga
    35521 aggtacagtg cttaaccaac gtttgtgttt atttcactgg ctacgacgtg gtttccgtct
    35581 gtgtccgtca tttattacca gccaattcac ccccgccctg aagagtgaac tgaagcggcg
    35641 cggaattgcg cgtaactttt acgacgatac caatctacaa gcgttagtga atctctgctc
    35701 ccgacggctg caaaaacgct ttgaaacgcg cgatattcat ttcctgtgtc tgtatctgca
    35761 atattgtttg ctgcaacacc acgctggaat tacgcctcag tttaatccgc tccaacgtcg
    35821 ctgggcggaa tcctgccttg aatttcaggt agcgcaggaa attggacgcc actggcagcg
    35881 tcgggcgctc cagcctgtac cgcctgatga gccactgttt atggcattac ttttttccat
    35941 gttgcgggtt cccgatccat tgcgtgatgc gcatcagcga gacagacaat tgcgtcagtc
    36001 tatcaaacgt ctggtaaacc attttcgtga gctgggaaat gttcgttttt atgatgaaca
    36061 ggggttatgc gatcagcttt atacccacct cgcccaggcg ttaaatcgca gtttgtttgc
    36121 catcggtatt gataataccc tgccggaaga gttcgccaga ctgtacccac gcctggtgcg
    36181 caccacccgc gcggcgctgg ccggatttga aagtgaatac ggcgtccatc tttctgatga
    36241 ggaaagcggt ctggtcgcgg tgattttcgg tgcctggcta atgcaggaaa acgatctgca
    36301 tgaaaaacag attattctac tgaccgggaa tgatagcgag cgagaagcgc agattgagca
    36361 gcagttacgc gaactaacgt tactgccgct caacattaag catatgtcgg taaaggcatt
    36421 tttgcagaca ggcgctccgc gtggcgcggc actgattatt gcgccttata ccatgccatt
    36481 accgctcttt tcaccaccgc tgatctatac ggacctgacg ctgacaacac atcaacagga
    36541 gcagatccgc aaaatgctcg aatcagcatg agggaacaac tttcgggcgc aaaaacattg
    36601 ccggaagcgc caccagcgcc attacccaga acacgccgtg gcccagatat tgatacagga
    36661 aaccggcgaa aacggtcatg atagcgatac tgccgcccat cgcgacggca gagtaaaccg
    36721 cctgtaaacg gatgacttcg ctaccctggc gaacagcaat atagcgcatg gcggccaggt
    36781 ggcagaccgt gaaggtgccg caatgcaaaa tttgcaccac tatcaaccac ggcaacgccg
    36841 tagttgctcc cataatgccc cagcgcacta cgccgcaaat cgccgagatc aacagcatat
    36901 cgcgagcact acaacggcgg aaaagtttat tactcagcgc aaagataatg acttccgcca
    36961 ccacgcccag cgaccacaaa taccccaccg ccgaggccga gtagccagct gcctgccagt
    37021 aaatggcgct aaaaccgtaa taggccgaat gtgccccctg caataaacaa acgcaggcca
    37081 gaaagcgcca gttctggcga accagcgcca accacgcaga ccaaccggtg ctctcctgct
    37141 ggcgacttgc cccttgtggc tgaatcgtcg gacggatgag aaagccgagc agcatggatg
    37201 ccacgcccaa cgtcaacagc gcgaggatca cccgataatc aaacatagtg accagtttgc
    37261 ccgtcagcgc cgagccaatg acaaacgcca ccgagcccca cagtcgcact ttgccgtaat
    37321 caagcgggaa ctgcttttgc cacgtattcg ccagtgcatc ggtcaacggt accagcggtg
    37381 agaaaaagag gttaaagcca atcatcacca gcatcagcca cgctacgtgc gcccccgccc
    37441 agaaggcgac agcaaagaga agtgtcagca gtgccagcac gcgcaaggcg gaaatcaggc
    37501 gggaaggatc gctgacgcgg ggcgcgatga gcaaactccc gagaaaacgg gcaaccagac
    37561 ctgcccccaa taacaggccg atggtttctg gcgttaaacc aatcccttta agccagacgc
    37621 tccagaaagg tagaaaaatg ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc
    37681 agcgcgtgga ttgcaaaacc atgagtccct cccgtcagga ggcgttagtc tggggttaat
    37741 cgaatgttgg tgcaagttga aaaataagaa tattcgagcc tgcttcccga tgtctgtaac
    37801 gccagttcac aagcactaaa aaactgcgat ccgcgacgca tgttgcaaaa ataatgcgta
    37861 attatgccgt tacgcttgcc aaacgttcct gcacaatggc gatgaaatcg cgtaatgccg
    37921 gcttcatctc ccctttcttc cacgccatca gtaaagcaat ggaaggcaca tttccggcaa
    37981 tgggacgaaa aacaacctgc ccggtattaa aattattcat ataaccgggt atcaaagtga
    38041 cgcccagccc catgcccacc agattcatgg tcaccagaat attcgttgcc acctggacga
    38101 tatttggctg gctgttttct tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg
    38161 aatacgccgg atcggtactg acgaaattca cgccatccag ttgcgcggcg gtgatctctt
    38221 tttcatgtgc taaagggtga tcaaccggta acaccaccac taatggttcg tcaaaaagct
    38281 ccagataatc aatctccggg ctataaacag gatggcgcat taagccgacg tcgagttcac
    38341 cacggcgaat tttttcctcc tgttgcgtgg tgattaaact caccagctca atcaaggtgt
    38401 ctggctgtct gagacgaaac attggtaata cttttggcaa taaattcact tccgccgatg
    38461 gcacaaagcc aatggttaat tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg
    38521 cattttccgc ttgttcgagg attgccagcg cgtcctggag aaaacattct cccgccgccg
    38581 tcaacgcgac tttgcgctta tccctcacca gtaacggaac accgacacag ttttcaagat
    38641 cgcggatctg gctgcttaac gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa
    38701 agttcagtgc ctgcgccact gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc
    38761 ccttgttatc gaaaaaacgt ctcagctggt agaaattaac tatttcacat attagcaacc
    38821 aaccagcaac atccttatga cacaaaaata gaaggtcaat acatcttatc tttcaggatt
    38881 aaaaaatatg accacaccct cagatttaaa catttaccaa ctgattgata cccaaaatgg
    38941 tcgggtcact ccgcgtattt ataccgaccc agatatttac caactggagc ttgaacgtat
    39001 tttcggccgt tgctggttat ttctcgccca cgaaagccag atcccaaaac ctggtgattt
    39061 ctttaacacc tacatggggg aagatgcggt tgtcgtagtg cgtcagaaag acggcagcat
    39121 taaggcgttt ctcaaccaat gccgccaccg ggccatgcgt gtgagttatg cagattgcgg
    39181 caacactcgc gcctttacct gtccgtatca cggctggtct tatggcatta acggcgagtt
    39241 gatcgatgta ccgctggaac ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg
    39301 actaaacgaa gttccttgtg tggagagtta taaagggctg atttttggca actgggatac
    39361 cagcgcaccg ggcctgcgtg attacctggg tgacattgcc tggtatctgg atggcatgct
    39421 ggatcgtcgc gaaggcggca ccgaaattgt cggcggcgta caaaagtggg tgatcaactg
    39481 taactggaaa ttcccggcag agcagttcgc cagtgaccag tatcatgctc tgttcagcca
    39541 tgcttctgcc gttcaggtat taggggcgaa agatgatggc agcgataagc gcctcggtga
    39601 tggacaaacc gcccgcccgg tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga
    39661 cggtcacggt agcggtttct tctttactga aaaaccggat gctaatgtct gggtcgatgg
    39721 cgcagtttca agctattacc gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt
    39781 tcgcgccctg cgcctggcgg gtcataacaa tattttcccc acgctttcat ggctcaacgg
    39841 cactgccacg ctccgcgtct ggcatccgcg cggccctgat caagttgaag tgtgggcgtt
    39901 ctgtattact gacaaagccg cctccgatga agttaaagcc gcttttgaaa acagcgccac
    39961 tcgtgctttt ggtcctgctg gttttctcga gcaggatgac tcggagaact ggtgtgaaat
    40021 ccagaaattg cttaaaggcc accgcgcccg caacagcaaa ctgtgtctgg aaatggggct
    40081 tggtcaggaa aagcgccgcg acgacggcat tcctggcatt actaactata tcttttcaga
    40141 aactgccgct cgtggaatgt accaacgctg ggccgatctt ctgagtagcg aaagctggca
    40201 ggaagtgctc gataaaaccg ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt
    40261 tcactagagt tacatcaccg cattagccag tttctctttc acgaagccag cttactggac
    40321 gactggaaat ttcgtgactg gctggagcag ctcgacaaag agattcgtta caccatgcgc
    40381 accacagtta acgcgcaaac acgcgaccgc cgcaaaggcg tccagccacc gacaacctgg
    40441 atttttaatg acaccaaaga ccagctggag cggcgaatcg cccgtctgga aacgggcatg
    40501 gcctgggcag aagagccgcc gtcacgcacc cgtcacttaa tcagcaactg ccagataagc
    40561 gaaaccgaca tcccaaacgt atttgctgtg cgggtaaatt atctgcttta tcgggcacaa
    40621 aaagagcgcg atgaaacatt ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa
    40681 gatgacaact ggcgcttgct ggaacgggat atcgtcctgg atcaagcggt aatcacttcc
    40741 cataacctga gtgtactgtt ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc
    40801 cggagggtga ggctctccgg atcgatacct cgcccgtcat cgccctgttc aacgttggcg
    40861 gcgagtttta tgccattaac gatcgttgca gccatggtaa tgcgtcgatg tcagaagggt
    40921 atctggaaga tgacgccacg gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa
    40981 cggggaaagc gttatgcctg cccgccaccg atccgctcac cacttatcca gtacacgttg
    41041 aaggtggtga cattttcatc gacttaccgg aggcgcagcc atgagcgatc tgcataacga
    41101 gtccattttt attaccggcg gcggatcggg attagggctg gcgctggtcg agcgatttat
    41161 cgaagaaggc gcgcaagttg ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg
    41221 tcagcgattt ggcgaacata ttctggcggt ggaaggtaac gtgacctgtt atgccgatta
    41281 tcaacgcgcg gtcgatcaga tcctgactcg ttccggcaag ctggattgtt ttatcggcaa
    41341 tgcaggcatc tgggatcaca atgcctcact ggttaatact cccgcagaga cgctcgaaac
    41401 cggcttccac gagctgttta acgtcaacgt actcggttac ctgctgggcg caaaagcctg
    41461 cgctccggcg ttaatcgcca gtgaaggcag catgattttc acactgtcaa atgccgccag
    41521 gtatcctggc ggcggtggcc cgctgtacac cgccagtaaa catgccgcaa ccggacttat
    41581 tcgccaactg gcttatgaac tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg
    41641 tatggccagc gacctgcgcg gcccacaggc gctcgggcaa agtgaaacct cgataatgca
    41701 gtctctgacg ccggagaaaa ttgccgccat tttaccgctg caatttttcc cgcaaccggc
    41761 ggattttacg gggccgtatg tgatgttggc atcgcggcgc aataatcgcg cattaagcgg
    41821 tgtgatgatc aacgctgatg cgggtttagc gattcgcggc attcgccacg tagcggctgg
    41881 gctggatctt taaggaagca cgatgaaaga aaaaacgatc attattgtcg gtggcgggca
    41941 agcggcggca atggctgcgg cctcgctacg ccagcaaggg ttcaccggtg agctgcatct
    42001 gttttccgat gagcgacatc ttccttatga acgacctcca ctctcgaaat ccatgttgct
    42061 ggaagattcc ccgcagttac agcaggtgtt acccgctaac tggtggcagg aaaacaatgt
    42121 tcatctgcat tccggtgtaa ccatcaaaac gctgggtcgc gacacacgag agttagtgtt
    42181 aaccaacggc gaaagctggc actgggatca gctttttata gcaatcggcg cggcagctcg
    42241 accgctgccg ttgcttgatg cactgggaga acgctgcttt accctacgcc atgccggtga
    42301 tgccgccaga ctgcgagaag ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg
    42361 aactattggt ctggaactgg ctgccagcgc cacgcagcgc agatgtaagg tgacagtgat
    42421 tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt
    42481 acagcgccac cagcaggctg gtgtgcgcat tctgctcaat aatgccattg aacatgtggt
    42541 cgatggtgaa aaagtagaac tgacgctgca aagtggggaa acgcttcagg ctgatgtggt
    42601 gatttacggt attggtatca gcgccaacga gcaactggct cgcgaggcca accttgatac
    42661 tgccaatggc attgtcattg atgaggcttg ccgcacctgc gatcccgcga tctttgccgg
    42721 tggcgatgtg gcaatcactc gtcttgataa tggtgcacta caccgctgcg aaagctggga
    42781 aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg ttagggctac cgctaccgct
    42841 actgccgccg ccgtggttct ggagcgatca gtacagtgat aacttacagt ttattggcga
    42901 tatgcgtggc gatgactggc tttgtcgtgg caacccggaa actcagaagg cgatttggtt
    42961 taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg aatcaggggc gtgagattcg
    43021 cccaattcgc aaatggatcc agagcggcaa aacgtttgat gcgaaactgc tgatagatga
    43081 gaacatcgcg cttaaatcac tgtaaccagg ataattagcg aatatctcaa cgcctggggc
    43141 gtggcgaggt gcaagagtgt gtattacgtt taaatcacat tatcttgcaa agggattggt
    43201 tatgaacaca cta
//
LOCUS       DAZPJP010000014        42132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPJP010000014 DAZPJP010000000
VERSION     DAZPJP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.014"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHU9J_000690"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHU9J_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1904324.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHU9J_000691"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHU9J_000691"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1904325.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHU9J_000692"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHU9J_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1904326.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHU9J_000693"
     CDS             4216..4620
                     /locus_tag="ACHU9J_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1904327.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHU9J_000694"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHU9J_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1904328.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHU9J_000695"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHU9J_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1904329.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHU9J_000696"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHU9J_000696"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1904330.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHU9J_000697"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHU9J_000697"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1904331.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9471
                     /locus_tag="ACHU9J_000698"
                     /pseudo
     CDS             8043..9471
                     /locus_tag="ACHU9J_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9541..10317
                     /gene="yaaA"
                     /locus_tag="ACHU9J_000699"
     CDS             9541..10317
                     /gene="yaaA"
                     /locus_tag="ACHU9J_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1904332.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10470..10766)
                     /locus_tag="ACHU9J_000700"
     CDS             complement(10470..10766)
                     /locus_tag="ACHU9J_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1904333.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10980..12266)
                     /gene="thrC"
                     /locus_tag="ACHU9J_000701"
     CDS             complement(10980..12266)
                     /gene="thrC"
                     /locus_tag="ACHU9J_000701"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1904334.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12267..13199)
                     /gene="thrB"
                     /locus_tag="ACHU9J_000702"
     CDS             complement(12267..13199)
                     /gene="thrB"
                     /locus_tag="ACHU9J_000702"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1904335.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13201..15663)
                     /gene="thrA"
                     /locus_tag="ACHU9J_000703"
     CDS             complement(13201..15663)
                     /gene="thrA"
                     /locus_tag="ACHU9J_000703"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1904336.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15690..15809)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15745..15810)
                     /gene="thrL"
                     /locus_tag="ACHU9J_000704"
     CDS             complement(15745..15810)
                     /gene="thrL"
                     /locus_tag="ACHU9J_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1904337.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16024..16710)
                     /locus_tag="ACHU9J_000705"
     CDS             complement(16024..16710)
                     /locus_tag="ACHU9J_000705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1904338.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17110..17250)
                     /gene="yjjY"
                     /locus_tag="ACHU9J_000706"
     CDS             complement(17110..17250)
                     /gene="yjjY"
                     /locus_tag="ACHU9J_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1904339.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17346..18062
                     /gene="arcA"
                     /locus_tag="ACHU9J_000707"
     CDS             17346..18062
                     /gene="arcA"
                     /locus_tag="ACHU9J_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1904340.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18122..19474)
                     /gene="creD"
                     /locus_tag="ACHU9J_000708"
     CDS             complement(18122..19474)
                     /gene="creD"
                     /locus_tag="ACHU9J_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1904341.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19532..20956)
                     /gene="creC"
                     /locus_tag="ACHU9J_000709"
     CDS             complement(19532..20956)
                     /gene="creC"
                     /locus_tag="ACHU9J_000709"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1904342.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20956..21645)
                     /gene="creB"
                     /locus_tag="ACHU9J_000710"
     CDS             complement(20956..21645)
                     /gene="creB"
                     /locus_tag="ACHU9J_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1904343.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21658..22131)
                     /gene="creA"
                     /locus_tag="ACHU9J_000711"
     CDS             complement(21658..22131)
                     /gene="creA"
                     /locus_tag="ACHU9J_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1904344.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22342..23211
                     /gene="robA"
                     /locus_tag="ACHU9J_000712"
     CDS             22342..23211
                     /gene="robA"
                     /locus_tag="ACHU9J_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1904345.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23208..23855)
                     /gene="gpmB"
                     /locus_tag="ACHU9J_000713"
     CDS             complement(23208..23855)
                     /gene="gpmB"
                     /locus_tag="ACHU9J_000713"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1904346.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23907..24428
                     /gene="yjjX"
                     /locus_tag="ACHU9J_000714"
     CDS             23907..24428
                     /gene="yjjX"
                     /locus_tag="ACHU9J_000714"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1904347.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24513..24839)
                     /gene="trpR"
                     /locus_tag="ACHU9J_000715"
     CDS             complement(24513..24839)
                     /gene="trpR"
                     /locus_tag="ACHU9J_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1904348.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24929..26866)
                     /gene="sltY"
                     /locus_tag="ACHU9J_000716"
     CDS             complement(24929..26866)
                     /gene="sltY"
                     /locus_tag="ACHU9J_000716"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1904349.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27077..28744
                     /gene="ettA"
                     /locus_tag="ACHU9J_000717"
     CDS             27077..28744
                     /gene="ettA"
                     /locus_tag="ACHU9J_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1904350.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28905..29012)
                     /gene="nadS"
                     /locus_tag="ACHU9J_000718"
                     /pseudo
     CDS             complement(28905..29012)
                     /gene="nadS"
                     /locus_tag="ACHU9J_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29051..30283)
                     /gene="nadR"
                     /locus_tag="ACHU9J_000719"
     CDS             complement(29051..30283)
                     /gene="nadR"
                     /locus_tag="ACHU9J_000719"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1904351.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30304..31686)
                     /gene="radA"
                     /locus_tag="ACHU9J_000720"
     CDS             complement(30304..31686)
                     /gene="radA"
                     /locus_tag="ACHU9J_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1904352.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31735..32703)
                     /gene="serB"
                     /locus_tag="ACHU9J_000721"
     CDS             complement(31735..32703)
                     /gene="serB"
                     /locus_tag="ACHU9J_000721"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1904353.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32809..33453
                     /locus_tag="ACHU9J_000722"
     CDS             32809..33453
                     /locus_tag="ACHU9J_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1904354.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33481..34497
                     /gene="lplA"
                     /locus_tag="ACHU9J_000723"
     CDS             33481..34497
                     /gene="lplA"
                     /locus_tag="ACHU9J_000723"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1904355.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34529..34792
                     /locus_tag="ACHU9J_000724"
                     /pseudo
     CDS             34529..34792
                     /locus_tag="ACHU9J_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34953..35672)
                     /gene="deoD"
                     /locus_tag="ACHU9J_000725"
     CDS             complement(34953..35672)
                     /gene="deoD"
                     /locus_tag="ACHU9J_000725"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1904356.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35752..36975)
                     /gene="deoB"
                     /locus_tag="ACHU9J_000726"
     CDS             complement(35752..36975)
                     /gene="deoB"
                     /locus_tag="ACHU9J_000726"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1904357.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37027..38349)
                     /gene="deoA"
                     /locus_tag="ACHU9J_000727"
     CDS             complement(37027..38349)
                     /gene="deoA"
                     /locus_tag="ACHU9J_000727"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1904358.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38476..39255)
                     /gene="deoC"
                     /locus_tag="ACHU9J_000728"
     CDS             complement(38476..39255)
                     /gene="deoC"
                     /locus_tag="ACHU9J_000728"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1904359.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39513..41063
                     /locus_tag="ACHU9J_000729"
     CDS             39513..41063
                     /locus_tag="ACHU9J_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1904360.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41035..41898
                     /locus_tag="ACHU9J_000730"
     CDS             41035..41898
                     /locus_tag="ACHU9J_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1904361.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9945 a        11409 c        10589 g        10189 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcaatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gtgccgcgtt
     8341 tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca caactttata aagaacgtga
     8401 cgtcaatgga cagtttcgtg gaggaccggc atggtatatg gcgcgcgggc tggggatgcg
     8461 ttggatgggc gttctgttcg ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg
     8521 agttcaggcg aacgccgttg cccgagccct gagttttctt ttgattttcc cccgctggtg
     8581 acaggcatta ttctcgctgt ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc
     8641 gcccggctca tgcaggggtt tgtcccgttg atggcgataa tctgggtgct gaccagcatg
     8701 gtgatttgcg taataaatat cgggcaactt ccccacgtca tttggtctat ttttgagagt
     8761 gcttttggct ggcaggaagc ggctggcggc gcggcgggat ataccttaag ccaggcgatt
     8821 actaacggtt ttcagcgcag tatgttttcc aatgaggcgg gaatgggttc gacgccaaac
     8881 gcggcagcgg cagcggcgtc ctggcctccg catccggcag cgcaagggat tgtccagatg
     8941 attggcattt ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg
     9001 gcgggtaacg acacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg
     9061 cgggtgctaa tgggttcctg gggtgctgag tttgttaccc tcgtggttat tctgtttgcc
     9121 ttcagctcca tcgttgccaa ctacatttat gccgaaaaca atctcttctt tttacgcctg
     9181 aacaacccta aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc
     9241 accttgctaa gtcttccgct gatgtggcaa ctggcagata tcataatggc ctgcatggct
     9301 attaccaatt tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat
     9361 tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat
     9421 atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta
     9481 tcgatcaact cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat
     9541 atgctgattc ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg
     9601 cgctatacgc tgccggagct gttagacaat tcccagcact tgatccatga agcgcggaaa
     9661 ctgacgcctc cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac
     9721 gccgctcgct ttcatgactg gcagccagat ttcacgccgg agaatgcccg ccaggctatt
     9781 ttggcgttta aaggtgatgt ctacaccggc ttgcaggccg aaactttcag cgaagacgat
     9841 ttcgattttg cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg
     9901 ctcgatttaa tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg
     9961 aaagatctgt atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca
    10021 gcacaaggcg ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag
    10081 ccgaagaaat tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa
    10141 tttaagataa tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt
    10201 gaaaatcggc tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt
    10261 gatgaagctt cctccagcaa tggcgaactg gtgtttaaac gctacgagca gcgttaagtt
    10321 tgcagtcaat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc
    10381 aatatattgc agaattcacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga
    10441 atgccggatt gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg
    10501 ccgtgatgat catgaggagc tttcttatgg tggcgcggcg gtggcggcgg tccgtgtggg
    10561 tgccagcgat tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga
    10621 cctccatccc agtaatagcc acgattatca cgatcgccta tctgtaattt tactgacggg
    10681 actaacgtaa tttccgcagc ctgtgctgcc atgggagcga ccagaaccag ggaaagtgcg
    10741 agtacgatag attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc
    10801 gatatccgga gattacgcga tgagaacgac ggttgttatt ctccgcaatc ctaatctcta
    10861 agttaccgca tttattgaga atttctcctt tttccctgtc gtttttctcc ataatttctt
    10921 cataaaaaag ccgggctgca taaaagcaaa cccggcctga ttgagataat gaatagattt
    10981 tactgatgat tcatcatcaa tttacgcaac gcagcaaaat cggcgggcag attatgtgaa
    11041 agcaagggta aatcagcacg ttctgccagc tcttttggca gatccaacgt ttcaccgaga
    11101 atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat
    11161 tcgcctggat tcaactggtc acacagcgca cgatacgcta cggcagcgtg cggctccgag
    11221 gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct
    11281 gcataaccca gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc
    11341 cagttgttcg gctgactcac gtccatcgcg ttggataacg tcgcctgagt cgctttgggt
    11401 gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt cgcagcaata
    11461 aaacgtttca ccggcagacc gagtgacttc gccagcagac ccgccgtcaa atcgccgaag
    11521 tttccgcttg gcaccgagac aaccagctgg ttgcgcgctt cctgcggcag ctgcgcaact
    11581 gcttcaaagt agtagcaaat ctgcgccagc aaacggctga tgttaatcga gttagccgag
    11641 tttaacccca gcgccacttt cagttcttca tcatcaaacg cctgcttcac cagcgcctga
    11701 caggcatcga aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa cgtacagaac
    11761 agtttttctt gcagtggact gattttgcct cgtggataga ggataaccac tttcacattc
    11821 ggcaaaccgt agaaagcatg agccactgcc gctccggtat caccggaggt cgcggtcaga
    11881 atggtcactg gcttatcgcc cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg
    11941 aaatctttaa atgccagcgt tggcccgtgg aacaattcca gacaaccgac atcgctttca
    12001 acattggcga ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc
    12061 tgcgggattt catcaccaat aaacgccgag aggatcttcg cactgcgggt gacaaaatcc
    12121 agcttcagca tctcatcaat ttcagtcagg ctgaattccg gcaggtcatg cggaaaaaac
    12181 agcccctgat ttttgcccaa cccctgggtt acggcttgcg caaagctgac ctgctcgttg
    12241 tgatctttca gattgtagag tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc
    12301 agccggcaaa tatgaacaaa accttcctga ttttgcaggt agttcttacc caaccagtcg
    12361 gcaacgcgct gggcggtatc cggcttgtca catagagcga acaaggtcgg gccggagccg
    12421 gagataccgc tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca
    12481 ggcagtaacc gttcacggta gggttcagcg ataacatctt tcatcagctt cgcggcaagc
    12541 tcaggctgac gggaatagca ggcgtgaatg aagccagcca gatgtcgccc gtgcgcaatg
    12601 caatcctggc ggcgatactg cgccggtaaa atagcccggg cttctgccgt cgagacttta
    12661 attcccggat acgccagcac ccacagccac tcatcaaacc ctggcacttg ctggctgatg
    12721 atgtcgtttt cttcgatcat caactgcata ccaccaagaa aacacggtgc cacgttgtcg
    12781 taatgaatgc tgccggagat acgtccttcc agctcgccca tcaaagccag caaacgggtg
    12841 tcattaagtg gcttgccgca gtgttcattc atcgccatca gcgccgcgac caccgaacag
    12901 gcgctggagc ctaagcccga gccgatcggc atattctttt ccagagtcat cgccactgga
    12961 atttgcttgc ccagctccag gcaaaaacgc tcccagcact gataaacgat attttcccgt
    13021 ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc
    13081 gcctcaaccg tgactacatc tccgagcaat gcaccatcaa cgggtgtcac cgccgccccg
    13141 agcacatcaa acccgacgct catattggca ctggaagccg gggcataaac tttaaccatg
    13201 tcagactcct aacttccatg agagggtacg tagcagatcg gcaaagacac cagcagcggt
    13261 aacgtcattg cccgcaccat atccgcgcag caccaacggc agcggctgat aatagtggct
    13321 ataaaaggcc agggcgtttt cgccattttt cactttgaac agcggatcat tcccatccac
    13381 ttcggcaatc ttcacgcggc agacaccatc ttcatcaata ttgccaacat agcgcaaaac
    13441 ttttccttca tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag
    13501 attcgccata aaagcggcaa catcaccctc ggcgttaaac tctgcgggca gcacaggttc
    13561 aatttcaata tccgccagct ccagttcacg tcccgtttca cgagcgagaa tcaatagttt
    13621 acgcgccaca tccataccag aaagatcatc tcgcgggtcc ggttcggtat aacccatttc
    13681 ccgcgccagc gtggtcgcct cggagaaact catgccttcg tctaacttgc cgaagatata
    13741 agaaagcgaa cctgaaagaa tgccggagaa cttcaccaat tcatcacctg cattgagcag
    13801 attttgcagg ttctcaataa ccggtaatcc agccccaacg ttggtgtcat agaggaattt
    13861 acgccgcgat ttttccgccg cataacgcaa ctgatgatag taatccatcg acgaggtgtt
    13921 ggcctttttg ttcggcgtga caacgtggaa accttcgcgc aggaagtcgg catattgatc
    13981 cgccactgcc tggctggagg tgcagtcaac aatgaccggg ttcagcagat gatattcttt
    14041 cacgaggcga attaagcgcc cgagattaaa cggctctttg gcttgtgcaa gttcttcctg
    14101 ccagttttcc agattaaggc catgcacact agtgagcaga gccttcgagt tggcaacgcc
    14161 gcagacacgt aagtcgatat gtttattctt cagccagctt tgctgacgct tcagttgctc
    14221 cagcagcgca ccgccaacgc caccgacgcc aatcacaaac acttcgataa cctgatcggt
    14281 attgaacagc atctgatgag taacgcgcac gccagtggtc gcatcatcgt tatttaccac
    14341 gacagagatt gagcgttcag aagatccctg agcaatggcg acaatgttga tattggcgcg
    14401 ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac catcacctac
    14461 caccgagata atggccagcc gttccatcac cgccagcggc tccagtaagc cttctttcag
    14521 ttccaggtag aactcttcct gcattgcccg ttcagcccgc acacagtcgc tttgcggaac
    14581 gcagaaactg atactgtatt cggaagatga ttgcgtaatc agcaccacgg aaatacgggc
    14641 gcgtgacatc gcggcaaaga cgcgcgccgc catgccgacc attcctttca tccccgggcc
    14701 ggaaacgctg aacattgcca tgttattcag attggaaatg cccttgaccg gtaattcgcc
    14761 ttcatcacga ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg tatttttaat
    14821 caggcaaggg atctggaact gggcgatggg ggtaatggtg cgggggtgaa gaactttagc
    14881 gccgaagtag gaaagctcca tcgcttcctg gtaggacatc gacttcaaca acctcgcatc
    14941 gggcacctga cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca
    15001 atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt tgcgtccaag
    15061 taccaccagt tcgccttttt cattaccggc ggtgaagcct gccatcagca ccatgtgatc
    15121 agccggaata cgacttgccg caatacggcg ggtggactca gcaatatcga cggtagattc
    15181 gaggtaatgc cccactgcca gtagtttttc gaccggatcg ataacggtaa cgttgtgacc
    15241 acgcgcttct aacacgccgg ccataatggc gatcgacatt ttctcgccac ggcaaatcag
    15301 cgcagcgtta atgctatccg ggcactgccc taacaaacta atgccatgca ggacatgttt
    15361 tatttgggca aattcctggt cgacgaaagt tttcaattgc gccagcggga atcccggctg
    15421 ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc tgatattggg
    15481 taaagcatcc tggccgctaa tggttttttc aatcatcgcc accaggtggt tggtgatttt
    15541 ggcgggggca gagaggacgg tggccacctg cccctgcctg gcattgcttt ccagaatatc
    15601 ggcaacccgc agaaaacgtt ctgcatttgc cactgatgta ccgccgaact tcaacactcg
    15661 catggttgtt acctcgttac ctttggtcga aaaaaaaagc ccgcactgtc aggtgcgggc
    15721 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
    15781 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
    15841 tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
    15901 tttactcatg gcaggtaacc agttcagaag ctgctatcag acactctttt tttaatccac
    15961 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
    16021 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
    16081 tcgttgctct aaaagcccca ggcgttgctg taaccagtcg accagtttta tgtcatctgc
    16141 cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg
    16201 gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat gttgctaatt gatagcaata
    16261 gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt
    16321 aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
    16381 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcggcacca gttctactgg
    16441 cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac
    16501 atcgtgtaac gattcagcca gtgttgggaa aactttaata ttatcaataa catcaccaga
    16561 tccatgtgcg acccagcggg tggctggctc caggtgtgcc tgactatcga caatccgcag
    16621 ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
    16681 gggtgcgacc aggataatcg ttatacgcat attgccactc ttcttgacca aataaccgcg
    16741 aacagggtga tcactgtcaa cttattacgc ggtgcgaatt tacaaattct taacgtaagt
    16801 cgcagaaaaa gccctttact tagcttaaaa aaggctaaac tatttcctga ctgtactaac
    16861 ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
    16921 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
    16981 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
    17041 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
    17101 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
    17161 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
    17221 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
    17281 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
    17341 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
    17401 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
    17461 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
    17521 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
    17581 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
    17641 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
    17701 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
    17761 tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt
    17821 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
    17881 ttcagtcccg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc
    17941 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
    18001 cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat ctggaagatt
    18061 aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt atttttcaac
    18121 cttatttcca gatacgtaat tcatcgtccg ttgtaacttc tttactggct ttcattttcg
    18181 gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca
    18241 ccaccagcac actggttccc aacaacagcg cgctatcgac agagttgagc agtccccaca
    18301 tcactccatc cagcaacaac agcgcgaggg taaacaacat gctattgcgc cagcctttca
    18361 ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg attatccatg
    18421 ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg
    18481 aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa
    18541 caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc gcccggtccg
    18601 ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgagg aagttttccc
    18661 agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt
    18721 gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggcaaa aaatcaccta
    18781 aaaaactggg atgcggccag ttgctggtta aggtcatttc gctattacgc ccggcaggca
    18841 ccacagaaag atcgccggta ccgcttaaat tcagggccat attcagcttc aggttctgct
    18901 tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg
    18961 tgccgggttc aatggtcagc gccgttccgt taacttcagg cgctttcacc acaccaatac
    19021 cacgcgcatc cccgacgcta atcacaataa atggcttgcc aaaggtgata tttggcgcgt
    19081 cgagttcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga
    19141 cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca
    19201 atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt
    19261 cctgtaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaaca agtttttgcg
    19321 gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga
    19381 caatcacctg ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaggg
    19441 tggtcatttt ccagaacagg gaggatttca acatagtctt ctcctttgca atggcagcag
    19501 gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg
    19561 aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg
    19621 cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca
    19681 aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat
    19741 ccagcacctt aagcgtgacg tgttcctgat ccacttcggc gcttagcgtt atacgaccgc
    19801 tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctga tccagtaacg
    19861 ccggttcagc agcaacatta acctcggtgg gcgtaacatg tagagtgatg tttttttctg
    19921 ccaactgcac ggtgcgcgct tcgctgacgc ggcggaataa tgccgccaca tcaacagcag
    19981 tcaaaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tccaccagtg
    20041 cctgcattcg cgcattttgc gtcagaatgt tgtcagtaaa acgagccact acttcaggcg
    20101 gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct
    20161 catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca
    20221 tactttccag cgcctgcgcg agtttacgca gctcgctact accgagatcg ggaagaggaa
    20281 cgggcttatt gtcagtgacg gaatcagcat agcgagtgag ccgggcaatg gagcggttga
    20341 tccaccaaac catgcctgcg ccaatcacca gtgcgatccc caacaaaatg gcgctggccc
    20401 ataaaattcg ccgctcgctg cgtttaatga ccggagccat cgccgcgttc ggtttgccta
    20461 cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg
    20521 aactttcggg atcggcagga ttttgcaacg tgctacgcgc accatactga ccacgcaacg
    20581 ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga
    20641 acaatacttt gccctgcgca tcggtcatat agacatgata ttcattgcgc actttgttaa
    20701 tgccaccgat attggcgcga aacgggcgat gttgtagctg attaaacgcc tgcgccagtt
    20761 gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg
    20821 cggtatcgat taacgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa
    20881 aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcagcaacc
    20941 gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat
    21001 cggagaaaga tcggggttga tggcgcgcag cttggcacgc aacgttttaa tgtgggtatc
    21061 gacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatta gttgctggcg
    21121 ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt
    21181 cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg
    21241 accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg
    21301 caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat
    21361 ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa
    21421 cgccggatgg agcgccagta actggcggca caattcaaag ccgctgatat caggcaaacc
    21481 aacatcgaga atcatgacgt cgggaacctg ctggcgagct ttatccagca ccggcaagcc
    21541 tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc
    21601 tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgcataaatc gcctctgtta
    21661 ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac
    21721 tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac
    21781 ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt
    21841 gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag aaatagccgc
    21901 atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt tcgcccggct
    21961 cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg
    22021 atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg catgagccaa
    22081 tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc
    22141 atttttatct attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac
    22201 aaaatctcaa tacttttatt tccgtcaagc cctaaaacat actctactaa gaaaaaaaca
    22261 ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg
    22321 ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg
    22381 ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat
    22441 tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat
    22501 attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt
    22561 ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag
    22621 aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt
    22681 attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt tgtcaccctg
    22741 gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct
    22801 gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc
    22861 attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa
    22921 caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg
    22981 gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga
    23041 accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat gctcaacctg
    23101 acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc caaagcggga
    23161 gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta acgctgcagc
    23221 tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac gacccagcca
    23281 gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt
    23341 aagcggcgct ctgcccatgc tggtaatccg agaatcgtac tcaccagaca tcccagtgca
    23401 ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt
    23461 gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccgtca
    23521 acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata
    23581 tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtca
    23641 cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg
    23701 ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca
    23761 cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc
    23821 gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa
    23881 cgttaatttt ctgagtaatg ctgattatgc accaagttat ctgtgcgacc accaatcccg
    23941 ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata
    24001 ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt gaggaaacgc
    24061 gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt
    24121 gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg gtgattgaaa
    24181 acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg gtgattctgc
    24241 agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg
    24301 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
    24361 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc gtgtatcagg
    24421 cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg atgcctgatg
    24481 cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc
    24541 agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc
    24601 gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc acgcaacagc
    24661 tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg
    24721 tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt
    24781 aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata
    24841 atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac tcgctaaaga
    24901 gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggcgctca
    24961 tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc agcacgttct
    25021 tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac
    25081 gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg
    25141 aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa
    25201 tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga
    25261 acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta
    25321 ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc
    25381 tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga
    25441 atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc
    25501 accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct
    25561 tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc
    25621 gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat
    25681 caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg tagaaaccac
    25741 gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca
    25801 ataaatccac ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg
    25861 ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc acacggcgtt
    25921 caatcaacga cgtggattgc gagcgcataa tgacgtcatc gcgccatttc gcctgctcgt
    25981 cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatctgat
    26041 cttcattaag ctgctgcacc tgggcaagcg acgggatcat cagccgtgca ttttcagcat
    26101 cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa tcggtcgctc
    26161 cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg
    26221 aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccagtgt
    26281 tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg
    26341 acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc
    26401 acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt
    26461 agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac
    26521 gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag
    26581 gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcagat
    26641 catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca
    26701 tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt tgcgcgtaac
    26761 ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga
    26821 caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta
    26881 agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg
    26941 ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg
    27001 ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    27061 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    27121 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    27181 tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcgggcat
    27241 tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc
    27301 gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    27361 tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga
    27421 tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca
    27481 cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac gtctgccgga
    27541 ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag cgttgtgccg
    27601 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    27661 cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg tggtggcgat
    27721 tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg
    27781 tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct
    27841 ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg
    27901 ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga
    27961 agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc
    28021 tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggtga
    28081 tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg tcgggatcat
    28141 cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc aggaacagcc
    28201 ggacagcggc accatcactt taggtgaaac ggtgaaactg gcgtcggttg atcagttccg
    28261 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
    28321 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    28381 ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc gtctgcatct
    28441 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    28501 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
    28561 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
    28621 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaagaa
    28681 acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc gtattgcgaa
    28741 gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa accaagaaat
    28801 ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg
    28861 agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaacagc cttagtatcc
    28921 gcaatttcgt agcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc
    28981 tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc tttgagccgt
    29041 ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga
    29101 aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca
    29161 tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac
    29221 catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat
    29281 caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg
    29341 tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact gcaaaatcaa
    29401 tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat gcgatctcat
    29461 caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga
    29521 agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca ccaaggatcg
    29581 ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg
    29641 ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagca
    29701 ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga
    29761 tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat
    29821 cccagccgtg cggatacggc tccatgccct cttcgttgaa agcatgaatg cgaatatttt
    29881 tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg ggctgctgcg
    29941 acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat
    30001 gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagcg
    30061 ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat tcaagcccca
    30121 aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta
    30181 actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc
    30241 cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc
    30301 tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg
    30361 catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc
    30421 gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga tctcccctgc
    30481 cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga
    30541 aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca cgccgccgac
    30601 cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc
    30661 cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg gattcgccat
    30721 catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac
    30781 caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg
    30841 gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc
    30901 gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat caccatccag
    30961 aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg agccatcttt
    31021 ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc gcgtcagata
    31081 agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat ccgccatatg
    31141 catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg caatcaggca
    31201 gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg
    31261 atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca gcgttttcat
    31321 ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg cacctgggtt
    31381 accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc ggtcgaactc
    31441 tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac
    31501 ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg
    31561 cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc
    31621 ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt
    31681 tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga gcaattactt
    31741 ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt
    31801 gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgctgc
    31861 tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg ccagcgggat
    31921 ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt ctacgatgtc
    31981 gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc
    32041 ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc cggaggcaat
    32101 cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag
    32161 cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac
    32221 acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc
    32281 cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat
    32341 ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc
    32401 cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg cggtgagtga
    32461 acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc
    32521 cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag
    32581 cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga
    32641 aagaggcaga cccggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg
    32701 cattaaggct cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta
    32761 tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca
    32821 aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg
    32881 gcactgatgc agggggcgtc atggtttagt caaaaccacc agcgacagcg taatccacag
    32941 ctggaagaac tggcccgcac cctggcgcgt caggtgacgc tgaacgttgc accgctgatg
    33001 cgtaccgact caccagatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa
    33061 agccgcatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc ccgttcaggc
    33121 gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcagg cggctatttt
    33181 aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac cgctcggcta tctgcgcctg
    33241 acactcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt
    33301 ttacgcctga tgttgctgct ctcactggca atcggcgtag tgctgacccg cacgctgcta
    33361 cagggtaaac gcacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg
    33421 ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt
    33481 atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg
    33541 gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat
    33601 gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg
    33661 atggaagaag ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac
    33721 gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc
    33781 tctacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga
    33841 cgtaacgatc tggtggtgaa aacagtcgaa ggcgaccgca aagtctcagg ctcggcctat
    33901 cgcgaaacca aagatcgtgg cttccaccac ggcactttgc tgctcaatgc cgaccttagc
    33961 cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca
    34021 gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt
    34081 tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc
    34141 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgtcagagt
    34201 agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    34261 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    34321 gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    34381 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    34441 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    34501 gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat
    34561 tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    34621 attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc taaaatagat
    34681 cgaaaagacg gaacggctta gctcaaacat caaaagcaaa atgggagaaa tttatggttg
    34741 tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat gaatagattc
    34801 gtgaatagat acttgaatgt attttatttt ttatccatat gaaaaataat agtaaatcta
    34861 taaatcagtt gctattgatg aatagatact aaaaagccgg agcagtcccc cactccggct
    34921 taacacgaca atcgcctttc agcgaaacac aattactctt tatcgcccag cagaacggat
    34981 tccagtgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc
    35041 tcgtgagtgc ggatgtggtc agacacagtg cagatggtca gggctttcgc gccaaattct
    35101 gcagcgacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc
    35161 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttacca
    35221 acgcgagcat caatgcccag tgctttagcc gcgtctaccg cgttacgcac catgtcaaag
    35281 tcagcgatag cggcaaagtc atgatcttta aaacggatgc ggttaacttt ggaatcggtg
    35341 caggcaccca taccgataac gacgtcgcgc agttttacgt gcggcagaac tgcgccacag
    35401 gaacccacgc ggataatttt cttcacgccg aaatcggtga tcagttcttt ggtgtagatg
    35461 gagcaggacg ggatacccat accgtgaccc attacggaaa ttttgcggcc tttgtaagta
    35521 ccggtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
    35581 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcgaaatcg
    35641 cccatttctg cattaatgtg tggggtagcc attgttttat ccttatcaac atgttttcac
    35701 cctctccctg agggagaggg ccggggtgag ggcatcaagc cgcacccatt atcagaacat
    35761 ggctttgcca tattccatat cagaagtacc aaaatatttt gccagagtct ggccgatatc
    35821 cgcgaaggtt tcacggtgac ccagtgagcc cggttttact ttcgggccgt aaaccagtac
    35881 cggaatgtgt tcacgcgtgt ggtcagtacc ggtccaggtc ggatcacaac cgtggtcagc
    35941 agtgaggatc aggatgtcgt catcgcgcag cagagacatc agctccggca gacggcggtc
    36001 gaacagctcc agacccacag cataaccggc gacgtcgcga cggtggcccc aggaagagtc
    36061 gaagtcaacg aagttggtga agacgatggt gttatcaccc gcttctttca tctctttgat
    36121 ggtggcgtca aacagcgcgt ccaggccagt tgctttcact ttcttggtga taccgcagtt
    36181 ggcgtagatg tctgcaattt taccgacaga aaccacctga ccgtgttttt catcaaccag
    36241 tttctgcagc acggtcggtg ctggcggctc aacagccagg tcgtgacggt taccggtacg
    36301 ctggaagtta ccggctttgt caccgataaa cggacgagcg ataacacgac cgatgttgta
    36361 gccgccgttg gtcagctctt cacgggcgat ttcgcacagt tcgtagagtt tatccaggcc
    36421 aaaggtttct tcgtggcagg caatctggaa cacagagtca gcggaggtat agaaaatcgg
    36481 cttgccggtt ttcatgtgct cttcgcccag ctgatccaga atgaccgtac cggaagagtg
    36541 gcagttaccg aggtaacccg gcagattagc gcgttcaacc agtttatcca gcagctcttg
    36601 cgggaagctg ttttcgtgat cggagaaata tccccactca aatagaaccg ggacaccggc
    36661 gatttcccag tgaccagacg gggtatcttt accggatgac atttcgtgcg cccatgcgta
    36721 cgcgccgata acttcagcgt tgccgtccat tcccgccgga atgaaaccgg tagaaccttc
    36781 gtgtgctttc gccagcccca gacgggtcag atttggcaga ttgagcggac ctttacgacc
    36841 gttatcagct tcgcctttgg cacaagcttc tgcgatatga cccagggtgt cagccccgac
    36901 gtcaccaaag cgttctgcat cttctgtagc gccgatgccg aatgagtcca gcaccataat
    36961 aaatgcacgt ttcatatgtt ctccgtactc agtgcttcaa atttaaatga tcagatcagt
    37021 ataccgttat tcgctgatac ggcgatagac agttggtgtg ctttccggtg ctttatcggc
    37081 aagtttaatt gccgctttca ccgctttcgc cgcttcctgc cagctgtttt cgtctttcgc
    37141 gtggataacc gccagcggac gctgaccgtc tacctggtcg cccaaacgcg ccatatcagt
    37201 aaagccgacg ctgtaatcaa tggtgtcaga tgcctgacga cgaccgccgc ccattgcaac
    37261 cactgccatc cccagcgcgc gggtatccat ttcactgaca aaaccttcgg tatcagcata
    37321 gactgctttc gtcagcatcg ctgtcggcag atacttcgcg tagttctcaa cgaagtcggt
    37381 tgggcctttt tgtgccgcta ccatacgacc aaagacttct gctgctttac cgttgtccag
    37441 cactgcttgc aatttcgcgc gcgcttcggc gtcatctttc gccagtttgc cggagataag
    37501 catctccacg cacagcgcca tcgtgacatc aaacagacgc gggttacgat actcacccgt
    37561 caggaactgc accgcttcac gaacttcaac cgcgttacct gcactggagg ccagtacctg
    37621 attcatgtcg gtgagcagcg cggtggtgcg cacgccagcg ccgttagcca cgccaacaat
    37681 cgcttcggca agggcttcag agagttcgta ggtcggcata aacgcgccgc tacccacttt
    37741 cacgtccatc accagcgcgt ccagaccttc cgcaagtttc ttcgccagaa tagaggcggt
    37801 gatcagcggg atggagtcca cggttgcggt aatatcacgg gtcgcgtaga aacgtttatc
    37861 cgccggagcc agtgagctgg tctgaccgat aatcgccacg ccgacgtctt taataatttc
    37921 gcggaaacgg ttgtcatccg ggaaaatgtc gaagccaggg atggattcca gtttgtcgag
    37981 cgtaccgcca gtatgaccga ggccgcgacc agagatcatc ggaatatagc cgccgcaggc
    38041 tgcgaccatc ggccccaaca tcagcgaagt cacatcgccg acgccaccag tggaatgttt
    38101 atcaacaatc gggccattca gatgcaggct tttccagtcg agaacggttc ctgaatctcg
    38161 catcgccatg gtcagcgaga cacgctcagg cattgtcata tcgtggaaga aaatggtcat
    38221 cgcgagggcg gcaatctgcc cttcggagat agtgttgtcg cgaatgccgt tgataaagaa
    38281 acgaatttct tcatcgctca gtgcatgacc atcacgtttt ttacgaataa tttcttgtgc
    38341 gagaaacaag gtaacctccc gaggaaaaga gtatgaggcg gcttttgccg gatgcgtcta
    38401 cgccttatcc ggcctacggg tagatacgtc tcgtaggcct gataagcgca gcgcatcagg
    38461 cgtaaagcat cttacttagt agctgctggc gctcttaccg tcaccgtggc ccagcgcttt
    38521 caacaggctt gccagcaggc tggaagcacc aaagcggtag tgacgcgcat ctgcccagtc
    38581 agcaccgaac agttcatctg cgatggcgag atatttctgc gcatcttccg cagtacgcac
    38641 gccgcccgct ggtttgaaac caacggtttt ttctacgccc atatcacgga tcacttccat
    38701 catgatgcgc gcgctttccg gcgtcgcgtt cacagccact ttaccggtag aggttttgat
    38761 gaagtccgca cccgctttga tggagatttc agacgcttta cggatcagcg cttcgtcttt
    38821 cagttcgccg gtttcgatga tcactttcag cagtacattc gctgccgcgc aagcctcttt
    38881 acaggctttc accaggtcaa aaccaacctg ctcgttaccc gccatcagcg cgcggtacgg
    38941 gaacacaacg tcaacttcat cagcaccgta ggcgattgcc gcacgggttt ctgccagcgc
    39001 gatttcgatg tcatcgttac cgtgtgggaa gttggttacc gtagcgattc ggatttccgg
    39061 ggtgccctgc tctttcagcg ttttgcgagc aatcgggata aagcgaggat agatacagat
    39121 agcggcggta ttgccgaccg gggttttggc ctgatgacac agagcaatta ctttctcgtc
    39181 ggtgtcgtcg tcattcaggg tggtcaggtc catcaatttc agtgcacgca ggctgcttgc
    39241 tttcagatca gtcatttcat tctcctggct tgtcgccaat aaaattcacc ttgcgagttt
    39301 gttagtattc taacatctac tccgcaacac acttcgatat acatcacaat taaggaaatc
    39361 tacttacaag tttgcatcac tgtaatgcga tctggttcaa ataattcact ttcaaatgaa
    39421 tgcgtcagtg gtggcaaacg catcaggatc aaagtgaaca tcacgaaact tcttacaatg
    39481 gcgcatcacc ttcggcataa aaggaaaaga agatgcccac ttctcatgaa aatgcactgc
    39541 aacaacgttg ccagcaaatt gtcaccagcc cagtgcttag cccggagcag aagcgccatt
    39601 ttctggcact ggaagcagaa aacaatctgc cttatccgca gcttcctgcc gaagcccgcc
    39661 gcgcgctgga tgagggtgta atctgcgata tgtttgaagg tcatgcgccg tacaaaccgc
    39721 gctatgtctt acccgattac gcccgttttc tggcgaacgg ttccgaatgg ctggagctgg
    39781 aaggcgcgaa agatcttgat gacgcactct ctctgctgac cattctttac caccacgtac
    39841 cgtcggtcac atcgatgccg gtctacctgg ggcaactgga tgcgttgttg caaccatatg
    39901 ttagaattct aacacaagac gagatcgatg ttcgaataaa acgtttctgg cgttacctcg
    39961 acagaaccct gccagacgcc tttatgcacg ccaatatcgg cccgtctgat tcgcccatta
    40021 cccgcgcgat cttacgtgca gacgcagagt tgaagcaggt ttcaccgaac ctgaccttta
    40081 tctacgatcc tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg
    40141 aatgtagcaa accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg
    40201 gctacgggat tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg
    40261 tacgccttaa cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc
    40321 gcactctacc gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc
    40381 tctatcaaca atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc
    40441 ctgaacgttt tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt
    40501 gtaagaaaga agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt
    40561 atcgcatcag cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc
    40621 aaaaacgcgc catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg
    40681 gcgcgcgttt gccgtatggt gatgagccag atccgatcac ccatctgcaa actgtcgccc
    40741 cgcatcatgc ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca
    40801 aacgtaatcc gcaggcgctg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg
    40861 aatttactgc caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt
    40921 tgtcggattt agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg
    40981 aagaagccgc acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac
    41041 agcagatgcg ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt
    41101 cgtctggctc tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg
    41161 acgatgggac gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg
    41221 cagattgttg acggcaaagt ggtgtggaac gctgtggttt gcgagcagtg tgatacctgc
    41281 ctgaagaggt gtccgcaaca tgccacgcca atggcgcaat ccatgagcgt ggacgaagtg
    41341 cttagccatg tccgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa
    41401 gccacgaccc aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa
    41461 ctgcgccacc tcacctgcct ggtggacagt aacggcatgt tgagcgaaac cggctgggaa
    41521 aaattactcc cggtgtgtga cggcgcaatg ctcgatctca aagcgtgggg gagcgaatgt
    41581 catcaacact taaccggacg cgataatcag cagattaagc gcagcatctg tttgcttgca
    41641 gagcgcggca agctggcgga actgcgtttg ctggtaattc cagaccaggt ggattatttg
    41701 catcacatcg atgagctggc gacgtttatc aagagacttg gcgatgttcc ggttcgcctg
    41761 aatgcgtttc atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg
    41821 gaagacgttg agccgttggc tgatgcgtta aaggtgcgcg gggtgagccg gttgatattt
    41881 ccggcgctct atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggca
    41941 tgataagacg cgacaagcgt cgcatcaggc attatgcgcc gactgccgga tgcggcgtga
    42001 acgccttatc cggcctacgg ttcggcacag acttgtaggc atgataagac gcgtcagcgt
    42061 cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc cggcctacgg
    42121 ttcggcacag ac
//
LOCUS       DAZPJP010000015        40129 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPJP010000015 DAZPJP010000000
VERSION     DAZPJP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.015"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHU9J_000731"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHU9J_000731"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1904362.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHU9J_000732"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHU9J_000732"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1904363.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHU9J_000733"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHU9J_000733"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1904364.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHU9J_000734"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHU9J_000734"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1904365.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHU9J_000735"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHU9J_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1904366.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHU9J_000736"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHU9J_000736"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1904367.1"
                     /translation="MKSCRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHU9J_000737"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHU9J_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1904368.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHU9J_000738"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHU9J_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1904369.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHU9J_000739"
     CDS             6537..6773
                     /locus_tag="ACHU9J_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1904370.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHU9J_000740"
     CDS             6776..7633
                     /locus_tag="ACHU9J_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1904371.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHU9J_000741"
     CDS             7654..9645
                     /locus_tag="ACHU9J_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1904372.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHU9J_000742"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHU9J_000742"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1904373.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHU9J_000743"
     CDS             complement(10248..11144)
                     /locus_tag="ACHU9J_000743"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1904374.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHU9J_000744"
     CDS             complement(11193..11432)
                     /locus_tag="ACHU9J_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1904375.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHU9J_000745"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHU9J_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1904376.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHU9J_000746"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHU9J_000746"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1904377.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHU9J_000747"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHU9J_000747"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1904378.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHU9J_000748"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHU9J_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1904379.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHU9J_000749"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHU9J_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHU9J_000750"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHU9J_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1904380.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHU9J_000751"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHU9J_000751"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1904381.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHU9J_000752"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHU9J_000752"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1904382.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHU9J_000753"
     CDS             complement(21570..22739)
                     /locus_tag="ACHU9J_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1904383.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHU9J_000754"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHU9J_000754"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1904384.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHU9J_000755"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHU9J_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1904385.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHU9J_000756"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHU9J_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1904386.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHU9J_000757"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHU9J_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1904387.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHU9J_000758"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHU9J_000758"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1904388.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHU9J_000759"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHU9J_000759"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1904389.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHU9J_000760"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHU9J_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1904390.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHU9J_000761"
     CDS             complement(30320..31576)
                     /locus_tag="ACHU9J_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1904391.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHU9J_000762"
     tRNA            31884..31960
                     /locus_tag="ACHU9J_000762"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHU9J_000763"
     tRNA            31965..32041
                     /locus_tag="ACHU9J_000763"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHU9J_000764"
     CDS             32149..32454
                     /locus_tag="ACHU9J_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1904392.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHU9J_000765"
                     /pseudo
     CDS             32580..34184
                     /locus_tag="ACHU9J_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHU9J_000766"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHU9J_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1904393.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEGGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHU9J_000767"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHU9J_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1904394.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHU9J_000768"
     CDS             complement(35794..36462)
                     /locus_tag="ACHU9J_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1904395.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHU9J_000769"
     CDS             complement(36526..37164)
                     /locus_tag="ACHU9J_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1904396.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHU9J_000770"
     CDS             complement(37177..39279)
                     /locus_tag="ACHU9J_000770"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1904397.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHU9J_000771"
     CDS             complement(39300..39926)
                     /locus_tag="ACHU9J_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1904398.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9629 a        10546 c         9889 g        10065 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcacgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ctaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatcctccct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtgatt attctgagcc cttaacatt
//
LOCUS       DAZPJP010000016        39861 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPJP010000016 DAZPJP010000000
VERSION     DAZPJP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.016"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(99..774)
                     /locus_tag="ACHU9J_000772"
                     /pseudo
     CDS             complement(99..774)
                     /locus_tag="ACHU9J_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(919..1527)
                     /locus_tag="ACHU9J_000773"
     CDS             complement(919..1527)
                     /locus_tag="ACHU9J_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1904399.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1711..2028)
                     /gene="metJ"
                     /locus_tag="ACHU9J_000774"
     CDS             complement(1711..2028)
                     /gene="metJ"
                     /locus_tag="ACHU9J_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1904400.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2305..3465
                     /gene="metB"
                     /locus_tag="ACHU9J_000775"
     CDS             2305..3465
                     /gene="metB"
                     /locus_tag="ACHU9J_000775"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1904401.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3468..5900
                     /gene="metL"
                     /locus_tag="ACHU9J_000776"
     CDS             3468..5900
                     /gene="metL"
                     /locus_tag="ACHU9J_000776"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1904402.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6039..6173)
                     /locus_tag="ACHU9J_000777"
     CDS             complement(6039..6173)
                     /locus_tag="ACHU9J_000777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904403.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6249..7139
                     /gene="metF"
                     /locus_tag="ACHU9J_000778"
     CDS             6249..7139
                     /gene="metF"
                     /locus_tag="ACHU9J_000778"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1904404.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7468..9648
                     /gene="katG"
                     /locus_tag="ACHU9J_000779"
     CDS             7468..9648
                     /gene="katG"
                     /locus_tag="ACHU9J_000779"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1904405.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9742..10647
                     /gene="yijE"
                     /locus_tag="ACHU9J_000780"
     CDS             9742..10647
                     /gene="yijE"
                     /locus_tag="ACHU9J_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1904406.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10674..11291)
                     /locus_tag="ACHU9J_000781"
     CDS             complement(10674..11291)
                     /locus_tag="ACHU9J_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1904407.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11565..12668)
                     /gene="gldA"
                     /locus_tag="ACHU9J_000782"
     CDS             complement(11565..12668)
                     /gene="gldA"
                     /locus_tag="ACHU9J_000782"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1904408.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12679..13341)
                     /gene="fsa"
                     /locus_tag="ACHU9J_000783"
     CDS             complement(12679..13341)
                     /gene="fsa"
                     /locus_tag="ACHU9J_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1904409.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13353..15854)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000784"
     CDS             complement(13353..15854)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000784"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1904410.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16163..17242
                     /locus_tag="ACHU9J_000785"
     CDS             16163..17242
                     /locus_tag="ACHU9J_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1904411.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17257..17577
                     /locus_tag="ACHU9J_000786"
                     /pseudo
     CDS             17257..17577
                     /locus_tag="ACHU9J_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17628..19924
                     /locus_tag="ACHU9J_000787"
                     /pseudo
     CDS             17628..19924
                     /locus_tag="ACHU9J_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19890..20768
                     /locus_tag="ACHU9J_000788"
     CDS             19890..20768
                     /locus_tag="ACHU9J_000788"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1904412.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20770..21111
                     /locus_tag="ACHU9J_000789"
     CDS             20770..21111
                     /locus_tag="ACHU9J_000789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1904413.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21098..21949)
                     /locus_tag="ACHU9J_000790"
     CDS             complement(21098..21949)
                     /locus_tag="ACHU9J_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1904414.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22164..23897)
                     /locus_tag="ACHU9J_000791"
     CDS             complement(22164..23897)
                     /locus_tag="ACHU9J_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1904415.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24080..26731)
                     /gene="ppc"
                     /locus_tag="ACHU9J_000792"
     CDS             complement(24080..26731)
                     /gene="ppc"
                     /locus_tag="ACHU9J_000792"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1904416.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27083..28234)
                     /gene="argE"
                     /locus_tag="ACHU9J_000793"
     CDS             complement(27083..28234)
                     /gene="argE"
                     /locus_tag="ACHU9J_000793"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1904417.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28388..29392
                     /gene="argC"
                     /locus_tag="ACHU9J_000794"
     CDS             28388..29392
                     /gene="argC"
                     /locus_tag="ACHU9J_000794"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1904418.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29400..30176
                     /gene="argB"
                     /locus_tag="ACHU9J_000795"
     CDS             29400..30176
                     /gene="argB"
                     /locus_tag="ACHU9J_000795"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1904419.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30237..31610
                     /gene="argH"
                     /locus_tag="ACHU9J_000796"
     CDS             30237..31610
                     /gene="argH"
                     /locus_tag="ACHU9J_000796"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1904420.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALTGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31877..32794
                     /gene="oxyR"
                     /locus_tag="ACHU9J_000797"
     CDS             31877..32794
                     /gene="oxyR"
                     /locus_tag="ACHU9J_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1904421.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32777..34177)
                     /gene="sthA"
                     /locus_tag="ACHU9J_000798"
     CDS             complement(32777..34177)
                     /gene="sthA"
                     /locus_tag="ACHU9J_000798"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1904422.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34498..35148
                     /gene="fabR"
                     /locus_tag="ACHU9J_000799"
     CDS             34498..35148
                     /gene="fabR"
                     /locus_tag="ACHU9J_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1904423.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35148..35507
                     /locus_tag="ACHU9J_000800"
     CDS             35148..35507
                     /locus_tag="ACHU9J_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1904424.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35547..36647)
                     /gene="trmA"
                     /locus_tag="ACHU9J_000801"
     CDS             complement(35547..36647)
                     /gene="trmA"
                     /locus_tag="ACHU9J_000801"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1904425.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36761..36951
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37016..38860
                     /gene="btuB"
                     /locus_tag="ACHU9J_000802"
     CDS             37016..38860
                     /gene="btuB"
                     /locus_tag="ACHU9J_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1904426.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38805..39662
                     /gene="murI"
                     /locus_tag="ACHU9J_000803"
     CDS             38805..39662
                     /gene="murI"
                     /locus_tag="ACHU9J_000803"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1904427.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9089 a        10030 c        10998 g         9744 t
ORIGIN      
        1 agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt aatcatccaa ggaaagctta
       61 tcagaagggt gatgactcca attagtagaa catatatttt cgataaaagc accgatgact
      121 ttttcatgaa gttctacaga acgcgagaaa cagatggttc tgcgggccag acgtttgatt
      181 cgggttctca gggtcagatt gttacgctca atgtgctgag taaaaatctt accagtcaga
      241 tgcttctcct tcggtaactc tctagcgtaa ctaccccatt cgtcagtggt caacatgccg
      301 atggtaaaag ggtaagcagc gcccgcaatt cacgacaggt ttcgtcagtc cgggggccaa
      361 aagtgtaggc cagaacacca ccagttttcg tgttgtacac gtaccagagc cagtgctgtc
      421 gcgctttact gccgacaaag ctccattgct gactaagttt gcagatgagt gccacatcag
      481 catgagcgac cggggatgac gttattcttc gtggcgagag ttttttaaag ttcggatgac
      541 tgtattaatg ccgattttca gcgtacgggc agtatcgcga accccggcac cgttgaaagt
      601 catttcagcg atttggtctt taactcccgg tttacggggc tcgtaacagt aactgagctg
      661 aaatacccgg tgacagtcac ggccgcggaa tcgctcatgt ctttttgggt tctgcccgtg
      721 acggtaaacc tgaacggact ggcaacgggg gcaatgaagg ttaactgtga ccatgaaaca
      781 accttaaaag cgagtattat atccttaatt caactaattg gaggcatcac cgtttaccgg
      841 agcgacagct tgcggaaagg tcgtgcaact acaaagcaac aaacagacta cggcgggaat
      901 tatttttttc atcatccatt accacgaaaa cagcggccac tctttggcga ctgtggtgag
      961 ttgtggcgcg tcgaaaacgg cttgtggtga aactaccgtt tcctcaagcg gaatattttt
     1021 gccgtagcgt tctttgagct tagcctggac cagcgggttg ctgagatcga actctttcaa
     1081 cttctgctgc tcgcccacgc gcattcccag cgcgttctgg taaaccttcc acaccacttc
     1141 cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcatacggtt taccgagata
     1201 acgttttgcc gtttgcgcca gtttttgctg ttgttcaaca ctcagtccgc cttcaacgcg
     1261 gcgaacaacg tatttgccct tttcaccatg ggcgatccac tgcttgagcg gggtgtattt
     1321 caccgggccg accgcttcaa aaacgtaggg ctttttgttg cgtatcacca gcataccggt
     1381 gtggctataa tcggaatggg tcgccagttg gatcgcttta ctttgcgatg agcgagagat
     1441 ctgaaagatg atgtcgccgg tttgtggctg ccaggcaaag gcaggtacag aaaccagcag
     1501 gctgaggatc agcagcctgt ttttcatagt taaacgtcca tgtataaaaa gcggtgggtc
     1561 gcagacaacg tgctcgttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa
     1621 gttcgtgcaa attcaataaa ttgcaatatg acgtaggcct gataagcgta gcgcatcagg
     1681 cgattccact ccgcgccgct cttttttgct ttagtattcc cacgtctccg ggttaatccc
     1741 catctcacgc atgatctctt ttgccgcttc cgggatttcg tcgctgcgct ctttacgcag
     1801 atcggcatca tccggcaaag gttgcccggt aaaggcatgc agaaacgctt cgcacagcag
     1861 ctcgctgttg gtagcgtgac gcaggttgtt cacctgacga cgcgtgcgtt catcggtgag
     1921 gatttttaac accttaagag gaatggaaac cgtaatcttt ttgacttgtt cactcttctt
     1981 gccgtgctca gcgtatgggc tgatatattc gccgctccat tcagccatga gatacttaat
     2041 cctcttcgtc aataaattga gaccagacca cacaatttat gtgggtactg accgtaaacc
     2101 cgcatagttt accgtacagg cgttaccgtg acatcgtgta atgcacctgt cggcgtgata
     2161 atgcatataa ttttaacggc tatttgggat ttgctcaatc tatacgcaaa gaagtttaga
     2221 tgtccagatg tattgacgtc cattaacaca atgtttactc tggtgcctga catttcaccg
     2281 acaaagccca ggaaacttca tcacatgacg cgtaaacagg ccaccatcgc agtgcgtagc
     2341 gggttaaatg acgacgaaca gtatggttgc gttgtccctc ccatccatct ttccagcacc
     2401 tataacttta ccggatttaa tgaaccgcgc gcgcatgatt actcgcgtcg cggcaaccca
     2461 acgcgcgatg tggttcagcg tgcgctggca gaactggaag gcggtgctgg cgcagtactt
     2521 actaataccg gcatgtccgc gattcacctg gtaacgaccg tctttttgaa acctggcgat
     2581 ctgctggttg cgccgcacga ctgctacggc ggtagctatc gcctgttcga cagtctggcg
     2641 aaacgcggtt gctatcgcgt gttgtttgtt gatcaaggcg atgaacaggc attacgggca
     2701 gcgctggcag aaaaacccaa actggtactg gtagaaagcc caagtaatcc attgttacgc
     2761 gtcgtggata ttgcgaaaat ctgccatctg gcaagggaag tcggggcggt gagcgtggtg
     2821 gataacacct tcttaagccc ggcattacaa aatccgctgg cattaggtgc cgatctggtg
     2881 ttgcattcat gcacgaaata tctgaacggt cactctgacg tggtggctgg cgtggtgatt
     2941 gctaaagacc cggacgttgt cactgaactt gcctggtggg caaacaatat tggcgtgacg
     3001 ggcggcgcgt ttgacagcta tctgctgcta cgcgggttgc gaacgctggt gccgcgtatg
     3061 gagctggcgc agcgcaacgc gcaggcgatt gtgaaatatc tgcaaaccca gccgttggtg
     3121 aaaaaactgt atcacccgtc gctgccggaa aatcaggggc atgaaattgc cgcgcgtcag
     3181 caaaaaggct ttggcgcaat gttgagtttt gaactggatg gcgatgagca gacgctgcgt
     3241 cgtttcctga gcgggctgtc gttgtttacg ctggcggaat cattaggggg agtggaaagt
     3301 ttaatctctc acgccgcaac catgacacat gcaggcatgg caccagaagc gcgtgctgcc
     3361 gtcgggatct ccgagacgct gctgcgtatc tccaccggta ttgaagatgg cgaagattta
     3421 attgccgacc tggaaaatgg cttccgggct gcaaacaagg ggtaaaaatg agtgtgattg
     3481 cgcaggcagg ggcgaaaggt cgtcagctgc ataaatttgg tggcagtagt ctggctgatg
     3541 tgaagtgtta tttgcgtgtc gcgggcatta tggcggagta ctctcagcct gacgatatga
     3601 tggtggtttc cgccgccggt agcaccacta accagttgat taactggttg aaactaagcc
     3661 agaccgatcg tctctctgcg catcaggttc aacaaacgct gcgtcgctat cagtgcgatc
     3721 tgattagcgg tctgctaccc gctgaagaag ccgatagcct cattagcgct tttgtcagcg
     3781 atctggaacg cctggcggcg ctgctcgaca gcggtattaa cgacgcagtg tatgcggaag
     3841 tggtgggcca cggggaagta tggtcggcac gcctgatgtc tgcggtactt aatcaacaag
     3901 ggctgccagc ggcctggctt gatgcccgcg agtttttacg cgctgaacgc gccgcacaac
     3961 cgcaggttga tgaagggctt tcttacccgt tgctgcaaca gctactggtg caacatccgg
     4021 gcaaacgtct ggtggtgacc gggtttatca gccgtaacaa cgccggtgaa acggtgctgc
     4081 tggggcgtaa cggttccgac tattccgcga cacaaatcgg tgcgctggcg ggtgtttctc
     4141 gtgtaaccat ctggagcgac gtcgccgggg tatacagtgc cgacccgcgt aaagtgaaag
     4201 atgcctgtct gctgccgttg ctgcgtctgg atgaagccag cgaactggct cgtctggcgg
     4261 ctcccgttct tcacgcccgt actttacagc cggtttctgg cagcgaaatc gacctgcaac
     4321 tgcgctgtag ctacacgccg gatcaaggtt ccacgcgcat tgaacgcgtg ctggcctccg
     4381 gtactggtgc gcgtattgtc accagccacg atgatgtctg tttgattgag tttcaggtgc
     4441 ccgccagtca ggatttcaaa ctggcgcata aagagatcga ccaaatcctg aaacgcgcgc
     4501 aggtacgccc gctggcggtt ggcgtacata acgatcgcca gttgctgcaa ttttgctaca
     4561 cctcagaagt ggccgacagt gcgctgaaaa tcctcgacga agcgggatta cctggcgaac
     4621 tgcgcctgcg tcaggggctg gcgctggtgg cgatggtcgg tgcaggcgtc acccgtaacc
     4681 cgctgcattg ccaccgcttc tggcagcaac tgaaaggcca gccggtcgaa tttacctggc
     4741 agtccgatga cggcatcagc ctggtggcag tactgcgcac cggcccgacc gaaagcctga
     4801 ttcaggggct gcatcagtcc gtcttccgcg cagaaaaacg catcggcctg gtattgttcg
     4861 gtaagggcaa tatcggttcc cgttggctgg aactgttcgc ccgtgagcag agcacgcttt
     4921 cggcacgtac cgactttgaa tttgtgctgg caggcgtggt ggacagccgc cgcagtctgt
     4981 tgagctatga cgggctggac gccagccgcg cgttagcctt cttcaacgat gaagcggttg
     5041 agcaggatga agagtcgttg ttcctgtgga tgcgcgccca tccgtatgat gatttagtgg
     5101 tgctggacgt taccgccagc cagcagcttg ctgatcagta tcttgatttc gccagccacg
     5161 gtttccacgt catcagcgcc aacaaactgg cgggagccag cgacagcaat aaatatcgcc
     5221 agatccacga cgccttcgaa aaaaccgggc gtcactggct gtacaatgcc accgtcggtg
     5281 cgggcttacc gatcaaccac accgtgcgcg atctgatcga cagcggcgat actattttgt
     5341 cgatcagcgg gatcttctcc ggcacgctct cctggttgtt cctgcaattc gacggtagcg
     5401 tgccgtttac cgagctggtg gatcaggcgt ggcagcaggg cttaaccgaa cctgacccgc
     5461 gtgacgatct ctctggcaaa gacgtgatgc gcaagctggt gattctggcg cgtgaagcag
     5521 gttacaacat cgaaccggat caggtacgcg tggagtcgct ggtgcctgcc cattgcgaag
     5581 gcggcagcat cgaccatttc tttgaaaatg gcgatgaact taacgagcag atggtgcaac
     5641 ggctggaagc ggcccgcgaa atggggctgg tgctgcgcta cgtggcgcgt ttcgatgcca
     5701 atggcaaagc gcgtgtgggc gtggaagcgg tgcgtgaaga ccatccgctg gcatcactgc
     5761 tgccgtgcga taacgtcttt gccatcgaaa gccgctggta tcgcgataat ccgctggtga
     5821 tccgtgggcc tggcgctggg cgcgacgtca ccgccggggc gattcagtcg gatatcaacc
     5881 ggctggcaca gttgttgtaa tttcagaaat ttaataatgc ccggtactca tgttttcggg
     5941 tttatggttt ctaatgaaat atattgaatt atcataggat taggccggat taagcgttta
     6001 cgacgaatcc ggcaagaagc aataagtaca tggttagttt atatttgcag tccggtttgc
     6061 tttgcatacc ggattttctt tttcttacca tcctgaagtt ttttcatctt ccctgatttt
     6121 tcctcaccat cattggtcat ttttcggttg acgcccttcg gcttttcctt catctttaca
     6181 tctggacgtc taaacggata gatgtgcaca acacaacata taactacaag cgattgatga
     6241 ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag agcctggcag
     6301 aagtccaggg gcagattaac gtttcgttcg agtttttccc gccgcgtacc agtgaaatgg
     6361 agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag tttgtatcgg
     6421 tgacctatgg cgcgaactcc ggcgagcgcg accgtacgca cagcattatt aaaggcatta
     6481 aagatcgcac tggtctggaa gcggcaccgc atcttacttg cattgatgcg acgcccgacg
     6541 agttgcgcac cattgcacgc gactactgga ataacggtat tcgtcatatc gtggcgctgc
     6601 gtggcgatct gccgccggga agtggtaagc cagaaatgta tgcttctgac ctggtgacgc
     6661 tattaaaaga agtggcagat ttcgatatct ccgtggcggc gtatccggaa gttcacccgg
     6721 aagcaaaaag cgctcaggcg gatttgctta atctgaaacg caaagtcgat gccggagcca
     6781 accgcgcgat tactcagttc ttcttcgatg tcgaaagcta cctgcgtttt cgtgaccgct
     6841 gtgtatcggc gggcattgat gtggaaatta ttccgggaat tttgccggta tctaacttta
     6901 aacaggcgaa gaaatttgcc gatatgacca acgtgcgtat tccggcgtgg atggcgcaaa
     6961 tgttcgacgg cctggatgat gatgccgaaa cccgcaaact ggtgggcgcg aatattgcca
     7021 tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc tatacgctta
     7081 accgcgctga aatgagttac gcgatttgcc atacgctggg tgttcgacct ggtttataaa
     7141 tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag cacaaaatgc
     7201 tgcctcgaaa tgagggcggg aaaataaggt tgtcagcctt gttttctccc tcattacttg
     7261 aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg acataatcaa
     7321 aaaagcttaa ttacgatcaa tttgacctac atctctttaa ccaacaatat gtaagatctc
     7381 aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt gtatcgtaac
     7441 ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca taacacgaca
     7501 gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcggg ggcgggcaca
     7561 accactcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca acattctaat
     7621 cgttctaacc cgctgggtga ggactttgac taccgcaaag aattcagcaa attagattac
     7681 tacggcctga aaaaagatct gaaagccctg ctgacagaat ctcagccgtg gtggccagcc
     7741 gactggggca gctacgccgg tctgtttatt cgtatggcct ggcacggcgc aggtacttac
     7801 cgttcaatcg atggacgcgg cggcgcgggt cgtggtcagc aacgttttgc accgctgaac
     7861 tcctggccgg ataacgtaag cctcgataaa gcgcgtcgcc tgttgtggcc aatcaaacag
     7921 aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcaggtaa cgtggcgctg
     7981 gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt ctgggaaccg
     8041 gatctggatg ttaactgggg tgatgaaaaa gcctggctga ctcaccgtca tccggaagcg
     8101 ctggcgaaag caccgctggg tgcaaccgag atgggcctga tttacgttaa cccggaaggc
     8161 ccggatcaca gcggcgaacc gctttctgcg gcagcagcta tccgcgcgac cttcggcaac
     8221 atgggcatga acgacgaaga aaccgtggcg ctgattgcgg gtggtcatac gctgggtaaa
     8281 acccacggtg ccggtccgac atcaaacgta ggtcctgatc cagaagctgc accgattgaa
     8341 gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga tgccattacc
     8401 tctggtctgg aagttgtctg gacccagacg ccgacccagt ggagcaacta tttcttcgaa
     8461 aacctgttca aatatgagtg ggtacagacc cgcagtccgg ctggcgcaat ccagttcgaa
     8521 gcggtagacg caccggaaat tatcccggat ccattcgatc cgtcgaagaa acgtaaaccg
     8581 acgatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa gatctctcgt
     8641 cgtttcctca acgatccgca ggcgttcaac gaagcctttg cccgtgcctg gttcaaactg
     8701 acgcacaggg atatggggcc gaaatctcgc tacatcgggc cggaagtgcc gaaagaagat
     8761 ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca ggacattatc
     8821 gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagcgggt atcggtggcc
     8881 tgggcatctg cttctacctt ccgtggtggc gacaaacgcg gtggtgccaa cggtgcgcgt
     8941 ctggcattaa tgccgcagcg cgactgggat gtgaacgccg cagccgttcg tgctctgcct
     9001 gttctggaga aaatccagaa agagtctggt aaagcctcgc tggcggatat catcgtgctg
     9061 gctggtgtgg ttggtgttga gaaagccgca agcgccgcag gtttgagcat tcatgtaccg
     9121 tttgcgccgg gccgcgttga tgcgcgtcag gatcagactg acattgagat gttcgagctg
     9181 ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggatgt ttccaccacc
     9241 gagtcactgc tgatcgacaa agcccagcaa ctgaccctga ccgcgccgga aatgaccgcg
     9301 ctggtgggcg ggatgcgcgt actgggtgcc aacttcgatg gcagcaaaaa cggcgtcttc
     9361 actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga tatgcgttac
     9421 gagtggaaag cgaccgacga atcgaaagag ctgtttgaag gccgtgaccg cgaaaccggc
     9481 gaagtgaaat acactgccag ccgtgcggat ctggtatttg gttctaactc tgtcctgcgt
     9541 gcggtggctg aagtttacgc cagcagcgat gctcacgaga agtttgttaa agacttcgtg
     9601 gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc tgaccttctt
     9661 cagcgactgc ctttcaggca gtcgctgaag tttctttacc ggcgtatagt gtccacagga
     9721 aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact ggcaatcagt
     9781 ggcctggttg tgctcacact tatctggagt tatagctgga ttttcatgaa gcaagtcacc
     9841 agctacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc tctcgtttta
     9901 ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga caccgtttaa atacacctta
     9961 gccattgccc tgttacaaac ctgcgggatg gttggcctgg cgcagtgggc gctggtcagc
    10021 ggcggagcgg ggaaggtggc gatcctaagc tataccatgc cgttctgggt ggtgattttc
    10081 gccgcgttgt ttctcggtga acgcctgcga cgtgggcaat atttcgcgat actgattgcc
    10141 gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc gatgaaaagt
    10201 gccatgctgg caatcctctc cggcgtcagt tggggggcga gcgcgattgt tgctaaacgt
    10261 ctgtatgccc gtcatccgcg cgtggattta ttgtcgttaa catcctggca gatgctctat
    10321 gcggcgctgg tgatgagtgt ggtcgcttta ctggtgccgc aacgtgaaat tgactggcag
    10381 cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacggcact ggcgtggagc
    10441 ttatggttgt ttgtattgaa aaacttgcct gccagtattg ccagcttaag cacactggcc
    10501 gttcccgttt gcggcgtact cttttcctgg tggctgctcg gcgagaatcc gggggccgtt
    10561 gaaggtagcg gtattgtgct gattgtgatg gcactggcgc tggtgagccg taagaaaaaa
    10621 gaagccgtta gtgtaaaaag gatctgaatt ttttcttcat gtggggcgat cgcttattta
    10681 acaaaataac gataatgccc caccatgcgc cagctaaaca gcacatcttc ttcctgcgca
    10741 cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc atctgaaacc
    10801 accccaatat gtgccagccc gttatctaat cgccaggaga caatatcgcc cgcttgatag
    10861 tcactggggt tcttgctgat ggggcgtgtt ttatcgtggt gggtaaacca ggtttccaga
    10921 ttaggcaccc gacggtgatc gatgttgctg tccgggcgct ttaactgcca tttttgcggg
    10981 tactcggcaa aattcttcgc catatcttcg tgcaccagtt tctgcaaatc gaccttctgg
    11041 ctgcgcaatg cacggatcac cacatcggaa cataccccgc gtttttgcgg gacatcaccg
    11101 ccaggatagg taagctgcat atacgccgga tcgtaaaata gcgtgctgcc gatttgctgt
    11161 ctggcaccgt ctgcgatggc aaggttggta ttggcctgga tttgtaccac ggttggtgga
    11221 acggcgggag attttaagga gtggctggta aatgccgtta gcaggctgag cagcgccagt
    11281 gaagctttca tctggttcgt ccgtgaaaat aagagtcaca gattcgacct tccgggataa
    11341 cgattgttct gtagagaaaa tgtccagaag cggataaaaa actcctgaac aacgtgggag
    11401 tttcagatta ctggcacaga gtgccggatg cggcgtgaac gccttatctg tcctaaaaaa
    11461 caagaaaatt caatatattg cagagattgc gtaggcctga taagcgtagc gcatcaggca
    11521 attttgcgtt caaactcccg gacaagccgg gagtttggag taggttattc ccactcttgc
    11581 aggaaacgct gaccgtactg gtcggctacc agcagagcgg cgtaaacctg atctggcgtc
    11641 gcgccgccag gcatgttgtg gatggtttca cattctgcac atgccgcttc agccacaatt
    11701 cgcattttcg ccgggacatc ttctttaata tccagttgag cgagagttat tggcaaacct
    11761 accgcatggc taagtgcagc tacggtttcg atttcctcaa ccggtgcgtt ttccagaacc
    11821 agctgcgtca gcgtaccaaa tgccactttt tcaccgtgat aatagtgatg cgcgtccggg
    11881 atagcggtca ggccgttatg cactgcgtgc gccgcagcca gaccaccact ttcaaaacca
    11941 acaccgctca aataggtgtt cgcttcaatc acgcgctcca gcgccggagt cactacatgc
    12001 tgttcggcag caagcatcgc tttttcgcct tcttccagca gggtgttgta gcacagttca
    12061 gccagtgcca gcgcagcctg ggtgcacttg ccgcccgcca tggtggtcgc gccgctacga
    12121 gagcaggcac gcgcttcaaa ccaggttgcc agcgcatcgc cgatacccgc cgctaacaga
    12181 cgtgcaggtg cgccagcgac gattttggtg tcgacaatga ccatattcgg gttatttggc
    12241 aacagcagat agcggtcaaa ctcaccctca tcggtgtaga taacagacaa tgcgctgcac
    12301 ggtgcatcgg tagaggcgat agtcggtgcg atcgctaccg gaacacccat gaaatgtgcc
    12361 agtgctttgg ctgtatcgag ggtttttccg ccaccgatac cgagaattgc gccacactgc
    12421 gcagtctccg cgatgccacg cagacggtcg atctcatttt gcgaacatcc accgccaaac
    12481 ggcgcaattt ctactaccag tccagcatct ttaaagcttt tctcgacagt ggattgagca
    12541 aaacctaaaa caaatttgtc acccaccact aaccagcgtt ctgccagcgg cttcaggtat
    12601 tcgcccagac gattaatcac atcagcgccc tggatgtatt tacccggtga ttgaataatg
    12661 cggtccataa ttgctccttt agagatgagt agtgccaaat gcggcattcc agtcgtgttc
    12721 gaacttctct atagctgact ctaccgcagg ggtgttgagc atttgttgcg ctacatctaa
    12781 gggcagggtg atggattcac atcctgccag taaacagtcc agcgcctgac gcggcgtttt
    12841 aaagctggct gccagcacca tgctttctgg cgcgtgcatt tctaacagcg cttgcagctc
    12901 ctgaaccgta cgaatgccgt ctccgccctg ggcatctacg cggttaacat acggagcaac
    12961 gtattttgcc cctgccagcg cggccagtaa cccttgtgca gcactgtaca ctgcggtgcc
    13021 aagcgtagta ataccctctt ttttcagtat tttaattgct gccagacctt cggaagtcac
    13081 cgggattttc accacaatac ccggaatagc gtcgcgcagg cgcttcgctt cttccaccat
    13141 cccctgcgcg tcgcggctca tggtctgagc aaacagaatg ccctcatcac caatcgcttt
    13201 ttgcagacgc ggcagcactt cccatatgga ctccttgctg gcagcgataa tgctcgggtt
    13261 agttgtcacc ccggcaatgg ggaatatgcg tgccagacgt tcgacttctg cgacgttagc
    13321 ggtgtccaga tacagttcca tgatgtttcc ttttacagtt ccagttcatg ttgcagcagg
    13381 ctggcgatag cgtcggcaga ggcggcgtta accagcgcgt tacggaattc ttcgtgcatg
    13441 atgcggcgag cgaggcgcga gaaaatgcgc atgtgctgat cgcccgcagc gtgtttgttc
    13501 agggttaaca tgatgatgaa ttgcgcttca tcatcacccc agcgcaccgg cgcttgcaga
    13561 cgcgcaacgc tgatggtgga ttgctcaatg tgttctgatt tgctgtgtgg aatggcaaaa
    13621 ctaaagccca gaccggtaga gaaaacggcc tcgcgcgccc acaagtcggc ttccagttta
    13681 cgcggatagc ggcagcgccc tgccagcagc aggttatcgg tcatgccttt gagcacttct
    13741 tctttgctgc gccagtcgct ttccagtgtg atgcactcgg cggtgaccag cggtgcgtct
    13801 tgttgggtca tgcggaattg cgccagcagg tgctccactt ccagcgaagt acggcaggcc
    13861 attgcctggt tgagcaactt gcggcactca cggctatcaa gttgcgccat ccgcgctttc
    13921 gccgccggaa ttgatggtgc gctcatgctg agttcatcca gccctaagcc gaccagcaac
    13981 ggcagcacgg aacctttcgc tcccagctca ccgcacagac caatccattt accctggcga
    14041 tgcaccgcct gcacggcgta atcgagcgcc cgcaagaatg ccggattcag gctgttgtag
    14101 tgacgagtaa ccttagcgtt atcgcgatcc actgccagca gatattgcgt caggtcatta
    14161 ctaccaatac taaagaaatc aatctcttcg cagcattgat cgatgatgaa catcaccgac
    14221 ggcacttcca gcataatgcc gagctggatc ttctcatcaa acggaatgtg ttcgttacgt
    14281 agttgctgtt tggcttctgc cagtttttct ttcacccata agatctcttc cattgaggag
    14341 atcatcggga tcatgatttt caggctgccg tgagcggagg cgcgcaggat cgagcgtagt
    14401 tgtgtggtaa ataacgacgc gtattcttca taaatacgca cggcgcgata accgaggaac
    14461 gggtttgcct ctgcgggaat gtttagataa tcaacgggtt tgtcgccgcc aatgtccata
    14521 gtgcgcacaa taatgctgcg tccgttggca gactccagcg cctgacaaaa aatgttgtac
    14581 agctcgcttt cgcccggtgc actggtgcga tccatataga gcatttcagt gcggaacaaa
    14641 ccaacgcctt ccgcaccatt gccgaatgcg gcctgcgctt ccacggagtg agcgatgtta
    14701 gcggcaattt caatgcggat accgtcagcg gtacgggctt gttgggtcag ccagacacgc
    14761 tgttgctcac gcagggcgtc ctgtacgcgg gcttcttgct gataataacg agctacggct
    14821 tctcctggct caaccacaat cgccccggcg ttgccgtcga tataaatcgt ttgatgctgc
    14881 cacggagtga gggcatcaat atccacacca accagcgtcg gaatgttgaa cgaacgggca
    14941 aggatcaccg tatgtgaggt ggtgccgcca cttttcagca acagaccttt gaggtgattt
    15001 ttatccagtt cgaggaactg gctgggggtc agttcatcag ccatacaaat ggcggactgc
    15061 gtcagtttgc ccggtgccgg gaagcgttgc tcaccgtaga tttgctggag taactggaag
    15121 cagacgtcgc gtacgtccag ggcacgttct tgcaggtagc tgctgctgga acgggcgaac
    15181 tcttcgcaaa agtgattcgc actggcgaca atcgcttcgg cgcagcttaa tccctcgctg
    15241 acgcctgcca gtaagtgctg acgtaaagat gtatcgccag ccagggatcg gtgagcttcc
    15301 agtatcgcgc tggtagcacc gtcgctatcc agcagacgaa actcaatgtt tttcagtacc
    15361 agcgtcaggc cgttttccag tgtggattgc tcggcgtcaa cgtctttggc tgcgggaaga
    15421 ttactcagcg cattgagatc taaagaagag atcggcgtca gaatgccgcc tgcactaccg
    15481 ctgcacacgg tgcgggcgcg gataatttgc ggattcagat tggtcagtga aaccggcagt
    15541 ggttccagtt cgtcagattt aacttccgcc agcggcgcat cgcagtgggg gaattcatcg
    15601 cgcagccatt ggcttaaacg ctggtgcgct tcctgttcgt cggccccgga aatcagtaac
    15661 tggcagttat cgcccgccag cgtatcggtg ccaatcagcg caagggcgct tttggcgttg
    15721 cccttgcggt cagtgtgcag gttatgccac tcaatttgtg atgaaaaagt attacacagc
    15781 gtttcaacgt ggcttgccgg acgcgcatgt acgccgttag gtagctcaca aataaattcc
    15841 acaatcaggg ccattgcctc tctcccggta cggttttctg tttacaggat aagagcgcac
    15901 ggcaacggcc tgccatgtga caaatctgcc aaaagctgga caaatgtaat gtaaccgtca
    15961 atttgcgacg cgtctcacaa gacgccgttt tgcggcatgc ttccggttta tcgcaagtta
    16021 tgaggcggat cgcatttttg tactgatatt acaaaagtcc agtaaatggc ctttttatcc
    16081 actgtttgct ccgcctgcga ttgcctattg tttcctccat caaaaatatg gacatgggct
    16141 ttcgcaagcc cgggagcagc ttatgaatga gttggtgcag atcctgaaaa atacccgtca
    16201 gcatttaatg acgggcgttt cacacatgat tcccttcgtg gtatcgggcg gtattttgct
    16261 ggcggtttcc gtcatgttgt atggcaaagg cgcagtgccg gatgccgtag ccgatccaaa
    16321 tctgaaaaaa ctgtttgata tcggcgttgc gggcttgacg ctgatggtgc ctttcctcgc
    16381 cgcttacatt ggttattcca ttgcagaacg ttctgcgctg gctccatgcg ctatcggtac
    16441 ctgggttggt aacagctttg gtgcgggctt ctttggtgca ctgatcgccg ggattatcgg
    16501 cggcatcgtg gtgcattacc tgaagaaaat tccggtgcat aaagttctgc gctcggtgat
    16561 gcctatcttc attattccta tcgtcggcac actgattacc gcaggcatca tgatgtgggg
    16621 cttgggcgag cctgtagggg cgttgaccaa cagcctgact cagtggcttc aggggatgca
    16681 gcagggcagc attgttatgc tggcggtgat catgggtctg atgctggcgt tcgatatggg
    16741 cggtccggtt aacaaagtgg cctatgcctt catgctgatt tgcgttgctc agggtgttta
    16801 taccgtggtg gccatcgctg ccgtgggtat ttgtatcccg ccgctgggga tggggctggc
    16861 gacgctgatt ggtcgtaaaa atttctccgc agaagagcgc gaaaccggca aagcggcact
    16921 ggtgatgggc tgtgttggcg ttactgaagg ggcgattcct ttcgccgctg ccgatccgct
    16981 gcgtgtcatt ccttccatca tggtcggttc agtttgtggt gcagtaactg cggcgctggt
    17041 cggtgcgcag tgctatgcag gctggggtgg tctgattgtg ctgccggtgg ttgaaggcaa
    17101 gctgggttat atcgcagcag tggctgtcgg agcagtggtg acggctgttt gtgtgaacgt
    17161 gctgaaaagt ctggcgcgta aaaatgggtc ttcgactgat gaaaaagaag acgacctgga
    17221 tttggatttt gaaattaatt aattgaggaa cgagccatga cgaaaattat tgcagtaacc
    17281 gcatgtcctt caggtgttgc ccatacctat atggcagcag aggcgctgga aagtgccgcg
    17341 aaagcgaaag gctgggaagt gaaagtagaa acctaggggt caattggtct ggaaaatgaa
    17401 ctgactgcgg aagatgtggc gagcgctgat atggtgattc tgaccaaaga tatcggcatc
    17461 aagtttgaag agcgttttgc tggcaagacc atcgtgcgcg tcaacatcag cgatgcagta
    17521 aaacgtgctg acgccattat gagcaaaatt gaagctcacc tggcgcaaac cgcttaagtt
    17581 cttctcccgc tcgcaagggc gggttcgctt tcccacagga gttcctcatg acgaatcgta
    17641 tctctcgcct caaaactgca ctgtttgcca atacccgtga aatctcgctg gagcgggcgc
    17701 tgctttatac cgccagccat cggcaaaccg aaggcgaacc ggtgatattg cgccgggcga
    17761 aagcaacagc gtatatcctt gaacatgttg aaatttcgat tcgtgatgaa gaactgattg
    17821 ccggtaaccg caccgtaaaa ccgcgcgccg ggattatgtc gccggaaatg gacccttact
    17881 ggctgctgaa agagctggat caattcccga cgcgtccgca ggaccgcttt gctatcagcg
    17941 aagaagataa acgtatctac cgtgaagagt tgttcccgta ctgggaaaaa cgttcgatga
    18001 aagatttcat caacgggcag atgacagatg aagtaaaagc cgcgaccaac acgcagattt
    18061 tcagcatcaa ccagacggat aaaggccagg ggcacattat tattgattac ccacgcctgt
    18121 tgaatcacgg tctgggtgag ctggtggcac agatgcagca acattgtcag caacagccgg
    18181 agaatcactt ttatcaggcc gcgttgttac tgctggaagc ctcgcagaaa cacattttgc
    18241 gttacgccga actggcggaa acgatggcgg caaactgcac agatgcacag cgtcgcgaag
    18301 aactgctgac tattgcggag atctctcgcc ataacgccga acataagccg cagacgttct
    18361 ggcaggcgtg ccagttattc tggtacatga acatcattct gcaatacgaa tccaacgcca
    18421 gttcgctgtc tttagggcgc ttcgaccagt atatgttgcc gttctatcag gcatctttaa
    18481 cccagggcga agatccggcg ttcctgaaag aactgctcga atctttatgg gtgaaatgca
    18541 acgacatcgt gctgttgcgc tccaccagca gcgcgcgtta tttcgcaggt ttcccgaccg
    18601 gctataccgc actgctcggc gggttaaccg agaacggacg tagcgcggtg aacgtgcttt
    18661 cgttcctttg ccttgacgcc tatcaaagcg tgcaattacc gcaaccgaac ctcggcgtgc
    18721 gcactaacgc cttgatcgac acgccgttcc tgatgaaaac cgccgaaacc attcgcctcg
    18781 gcaccggtat tccgcaaatc tttaacgatg aagtggtggt accagcgttc ctcaaccgtg
    18841 gcgtttcgct ggaagatgcg cgcgactatt ccgtagtggg ctgtgtggaa ttatctattc
    18901 ccggcagaac ctacggcttg catgacatcg cgatgtttaa tctgctgaaa gtgatggaaa
    18961 tctgcctgca tgaaaatgaa ggcaatgctg cgctgactta tgaaggttta ctggagcaga
    19021 tccgcgccaa gatcagccac tacatcaccc tgatggttga gggcagcaat atttgtgata
    19081 tcggccatcg cgactgggca cctgtaccgc tgctctcgtc ttttatcagc gattgtctgg
    19141 aaaaaggtcg cgatattacc gatggcggcg cgcgttataa cttctccggc gtacagggga
    19201 tcggtatcgc caacctgagc gattctctcc atgcgttgaa agggatggtt tttgagcaac
    19261 agcgtttaag ttttgacgaa ttgctgtcgg tattaaaagc caactttgca acgccagaag
    19321 gcgaaaaagt ccgtgctcgc ttaattaacc gcttcgagaa atacggtaac gatatcgacg
    19381 aggtggataa catcagcgcc gaactgttac gccactactg caaagaagtg gaaaaatacc
    19441 agaacccgcg cgcggttact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    19501 gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac tggcagacgg
    19561 cggcttgtca cctatgctgg gccaggacgc acaagggcca acagcggtac tgaagtcagt
    19621 cagtaagctc gataatacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    19681 ggcgaccctg gaaggtgaag cgggattacg caaactggcc gacttcttac gggcgtttac
    19741 ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    19801 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    19861 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    19921 gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt
    19981 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    20041 gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta cgcagagagg
    20101 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggggcgt
    20161 ttgaacggat tggtcgcgat atcagccttg acgctctgga acgggaagtg atgaaagatg
    20221 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    20281 aggcggagtt tgctacccgt tttttacagc gactgcggct gtggggtgtg tcatgcgcca
    20341 ttgaaactgc cggagacgcg ccagcaagca agctgttacc gctggcgaaa ttgtgcgatg
    20401 aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    20461 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc
    20521 cgcgtttacc gttgatccct ggtttcacgc tcagcaggga gaatatgcag caggcgctgg
    20581 atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg
    20641 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    20701 cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    20761 gaggttaaaa tggcatacct ggtggcagta accgcctgcg taagcggcgt ggcgcatact
    20821 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    20881 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg
    20941 gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc
    21001 cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg
    21061 gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    21121 ctgtcagttg gctgtggtac tgccggcgat attccgacgg cgagcgttcg gtgtttttac
    21181 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    21241 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    21301 attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagagatag tttggtgaaa
    21361 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    21421 aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt tggctgccga
    21481 gcaaatcgcg gcagtggcta agcaggctgg ctacgataag ccttgccgtt tgctgctcct
    21541 gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctata cgtgggccgc
    21601 gtcgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    21661 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt ggtagcgggt gttttccatt
    21721 gtgggaaatt ccagccacca gcggcaacgt acagcacatc gccgggaact aacgttgctc
    21781 cagcgccggt atcaacaaac tcaccttcca gcacaatttc cagccgtgga aaatcgacct
    21841 gatacgcaag atcgggcacc gggccgttac tgctggcaaa gtaaatctgg cgcagggaca
    21901 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    21961 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    22021 gcctgagggg agttcgatgt aaacattgcg gcagttgtat tgccggatgt ggcgtaaacg
    22081 ccttatccag cctaccgccg cgatctgtag gccggataag acgcgtcaag cgtcgcatcc
    22141 ggcaaatagc gcctggctga taattactga ttacctacct ggtcgccata cggcagagtg
    22201 tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt agtttctttg
    22261 aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa gcccgccaaa
    22321 tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc ctgtgagaaa
    22381 tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg aatggtgtac
    22441 atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg cgtgtcgtaa
    22501 acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc cgctttaaag
    22561 tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc ataatcgtta
    22621 tatgattcca gctcttctgc gcttaatcct ggcggaacat gatcggtatt gccatcaaac
    22681 ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag cagatgaacg
    22741 atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt cagcacgttg
    22801 gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg cttgtcggtc
    22861 tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg gttggtgatc
    22921 cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg cgtcagatac
    22981 agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc aatggtgtac
    23041 ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt atgcagcgca
    23101 tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc gcgctgggtc
    23161 gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc tttgaaatta
    23221 gccagtggcg gcaaggcatt attttcattc agtaactttg ttagcgagtt tagttgctga
    23281 cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat acgcgaggcc
    23341 aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat ggcgatcgga
    23401 tgcagaatca agccataaag cagggcaaaa gagacaacat aacgccacgg ctttggaata
    23461 tagaccgggc gcaggcgtgt ccacagcaga actgccaccg ccgtataggc cagcgcgata
    23521 agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt ggtgttggtt
    23581 tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac gtagtagcat
    23641 agcgccgcca gagaggccgc ccatagcacc acgccgatta ctgcggcaat aattttaatc
    23701 cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag cgagtcgcga
    23761 atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag agtagagaaa
    23821 aaccaaaagt agagcagtgc ccaacccagg gctttccagc taaaaagggg tttagcctgg
    23881 acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga atgtaacgac
    23941 aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag acagaaatat
    24001 attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca aaagcacgag
    24061 ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc ccggcaatag
    24121 tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt tctgcctggc
    24181 gggagcggtg cagcaactcg gcctgcaata cgttcagcgg gtcggtgtaa atattccgta
    24241 gctgaataga ctctgcaatc cacggcagat cggccatcag atgggaatcg ttggcaatcg
    24301 ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct aacggccaca
    24361 gtgctttgtc taccagacgt tgatcatagt attccgccaa ccacaggtct gctttggcga
    24421 agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg cacatggctt
    24481 ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct gcacccagcc
    24541 aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg gcgcgcagcg
    24601 actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc agtttgccca
    24661 attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt tcacgtacgt
    24721 agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga cgccagctct
    24781 ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg taaagcgaca
    24841 ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg ccctgttcag
    24901 tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc gcatgggcag
    24961 gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc tcaatacccg
    25021 ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag gaagctgcca
    25081 tcacgcccgc atcttttgct gagtcggaat agccaatcat caccatctgt ttgccctgaa
    25141 tcaggccgcg ataccagtcg atattgagca gctgggtcat gacatcgttg gcgttgttca
    25201 ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata cccgcttctt
    25261 tcagcagcag gtggacagcc agtacgtcgg acggcgtttt cgccatcgag atcacgtagg
    25321 cggcaatgga gccttgcggc gcttcggcaa tcacctggca ggtatcgagc acttcgcgcg
    25381 tttcggcgct tggttgccag ttgcgcggca gaagcggacg tttggagttc agttcgcgga
    25441 tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta gtcgccgata ccgaggtagc
    25501 gggtcagctc gcccagcgct tcggtatggc gggtgctctc ctgacggata tcaatacgga
    25561 ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg ccgttggcga
    25621 taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt tcccacagtt
    25681 cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc aggcgcgctt
    25741 ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga tagcgatacg
    25801 gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct tcaaccatcg
    25861 acagttcaga aaccagaacc tgaatatctt tcaggaacag atcggtggct ttccagcgac
    25921 tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg tcgcggtcac
    25981 cgcccatcca cgaagtaaaa cggaccggaa caaattcgac gggcagtttg tagccgaggt
    26041 tctcttccag ttgttcgttc agttcgcgca agtaattcgg tacgccttgc cacaggctgt
    26101 tttctactac ggcaaagccc catttggctt catctaccgg gcttggacgc agcttacgga
    26161 tttcatcagt atgccatgac tgggcgatta actggcgcag gcgacgcatc agctggttgt
    26221 gttcgtagtc agcgatatct ttgttatcga gctgttttaa gcaggcgttc acttccacca
    26281 ttttgtggat cagtgtacga cgggtaattt cggttgggtg agccgtgagg accagttcca
    26341 gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc tggtttttca
    26401 gtttgcgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct ttcggcgaaa
    26461 tgctgtggta ttgctcggcg gtgttggcta ggttcaggaa ctgactaaac gcacgcgcaa
    26521 cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc tggcggttag
    26581 catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg cgttcaagaa
    26641 tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc atactgacat
    26701 tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat cttatcgttt
    26761 gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt gcttttcgtc
    26821 gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg cttcagcaaa
    26881 cgaataaata gcaggaattt acgccattaa attcacgacg ctttaaataa gcgtaactta
    26941 tggaaatgtt aaaaaatcgc catacataac accaaaggtg taggtcggat aagatgcgca
    27001 agcatcgcat ccgacattat tgcggcaccg gagtttggca acggtgccgg atgcggcgcg
    27061 agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc tggataatca
    27121 actcacgggt gggtttaata aaccgcgtct ccagatattc atcaggctga tgagcttgat
    27181 tgatggaacc cggaccgagc accagcgtcg ggcataacgt ttgaataaac ggcgcttcgg
    27241 tacagtaatt caccacttcg gtttttgctc cgagcaattt ctcaaccact tcaaccagtt
    27301 gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg accgtcagac
    27361 gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca ttaagttcat
    27421 tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa ctcacagcaa gcgcaaatac
    27481 ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat ggcacggtaa
    27541 atgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata tgcccgatgg
    27601 cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag tgccccgact
    27661 ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc ggttgtagtg
    27721 acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg gcaaaataac
    27781 gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc ggttttttca
    27841 gtttcgtgac gtcgacatcg cgtagcgtat caaggataaa cgcaaaaaag cctttcatgt
    27901 cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac ggatcgcgcg
    27961 tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc cgccagcaac aagccgccag
    28021 ccccctgtcc gatacttgcc agcatattga atttgttgcg agttcctggc acaggctgca
    28081 cttccacatt gaagcctaaa tctttaaacc agtccgccag cagagtgatt aaatctgcat
    28141 tgctttgatc gagtgcctct tccgtggcgc ttattgaagg tgtggcaatc agagcacggt
    28201 aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg gtcatgatag
    28261 tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg taataaaacc
    28321 caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa gaaggtgaat
    28381 agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg cagagctagt
    28441 gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt cagcgcaaag
    28501 caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca tcgttgagct
    28561 gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg tagtgtttct
    28621 cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag cgggctgcgt
    28681 ggtgttcgac ctttccggtg cgtttcgtgt taacgacgcc accttctatg aaaaatatta
    28741 cggctttacc catcaatacc cggaactgct ggaacaggca gcctacggtc tggcggagtg
    28801 gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccaggctgtt atccgacggc
    28861 ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca atcagtggcc
    28921 ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt aaggcagcga tttcaaatag
    28981 tttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc aaccagagat
    29041 cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt tcccgcgcgg
    29101 cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaggcgc aagtcgcgca
    29161 agtgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca aaggcgttcc
    29221 ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg ttcagggcga
    29281 gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg cacaagcggt
    29341 acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt aagggtgcaa
    29401 tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa gaggcgctgg
    29461 aacgtctgtt tagcacactg gtgaattatc gtgagtcaca tcagcgtccg ctggtgattg
    29521 tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg ccggtgaaaa
    29581 agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc ggagcactgg
    29641 cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt gcggccgtag
    29701 gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa gagttaggtc
    29761 atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg gagaacggtt
    29821 atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg aacgtcaatg
    29881 ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt ttgctctccg
    29941 acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc gccgcgaaag
    30001 cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa gtgaacgcgg
    30061 cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcctcctgg cgtcatgcgg
    30121 agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta gcttaagttt
    30181 tgttggccgg aggcgcagct ttccggcatt gaatttcaaa ataaggaaac agagttatgg
    30241 cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa ttcaacgact
    30301 cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg gcctggtcca
    30361 aagccctggt cacggtaggc gtgttaaccg cagaagagca ggcgcaactg gaagaggcgc
    30421 tgaacgtgct gctggaagat gttcgcgcca ggccacaaca aatccttgaa agcgacgccg
    30481 aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag ttaggcaaaa
    30541 agctgcatac cgggcgtagt cgtaacgatc aggtagcgac tgacctgaaa ctgtggtgca
    30601 aagataccgt tagcgagtta ctgacggcta accgacagct gcaatcggcg ctggtggaaa
    30661 ccgcacaaaa caatcaggac gcggtcatgc caggttacac tcacctgcaa cgcgcccagc
    30721 cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt gatgaaagcc
    30781 gtttgcagga tgcgcttaag cgtctggatg tcagcccatt aggctgtggc gcgctgacgg
    30841 gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt gcttcggcga
    30901 cccgtaacag tctggacagc gtttctgacc gtgaccatgt gttggaactg ctgtcggctg
    30961 ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc tttaacaccg
    31021 gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc cggttcatca ttaatgccgc
    31081 agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa atgcggtcgg gtgcagggtg
    31141 cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac aaagatatgc
    31201 aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc ctgcatatgg
    31261 cggcgctggt gctggacggc attcaggtga aacgtccacg ttgccaggaa gcggctcagc
    31321 agggttacgc caacgccacc gaactggcgg attacctggt ggcgaaaggc gtacctttcc
    31381 gcgaggcgca ccatatcgtg ggtgaagcgg tggtggaagc cattcgtcag ggcaaaccgc
    31441 tggaagatct gccgctcgac gagttgcaga aattcagccc ggtgattggc gaagatgtct
    31501 atccgatttt gtcgctgcaa tcgtgcctcg acaagcgtgc ggcaaaaggc ggcgtctcac
    31561 cgcagcaggt ggcccaagcg attgcttttg cgcaggcgcg gttggggtaa gaacatttat
    31621 atgtacaaat ttgagcctgg cttatcgccg ggctttttta tggcaaaaaa aagcggatcc
    31681 tggagatccg caaaagttca cgttggcttt agttattcga gttgagaaac tctcgaaacg
    31741 ggcagtgact tcaagggtta aaagaggtgc cgctccgttt ctgtgagcaa ttatcagtca
    31801 gaatgcttga tagggataat cgttcattgc tattctacct atcgccatga actatcgtgg
    31861 cgatggagga tggataatga atattcgtga tcttgagtac ctggtggcat tggctgaaca
    31921 ccgccatttt cggcgtgcgg cagattcctg ccacgttagc cagccgacgc ttagcgggca
    31981 aattcgtaag ctggaagatg agctgggcgt gatgttgctg gagcggacca gccgtaaagt
    32041 gttgttcacc caggcgggaa tgctgctggt ggatcaggcg cgtaccgtgc tgcgtgaggt
    32101 gaaagtcctt aaagagatgg caagccagca gggcgagacg atgtccggac cgctgcatat
    32161 tggtttgatt cccacggttg gaccgtacct gctaccgcat attatcccga tgctgcacca
    32221 gacctttcca aagctggaaa tgtatctgca tgaagcacag acccaccagt tactggcgca
    32281 actggacagt ggcaaactcg attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt
    32341 cattgaagtg ccgttgtttg atgagccaat gttgctggct atctatgaag atcacccgtg
    32401 ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg gaaaaactgc tgatgctgga
    32461 agatggtcac tgtttgcgcg atcaggcaat gggtttctgt tttgaagccg gggcggatga
    32521 agatacacac ttccgcgcga ccagcctgga gacgctgcgc aacatggtgg cggcaggtag
    32581 cgggatcact ttactgccag cgctggctgt gccgccggag cgcaaacgcg atggggttgt
    32641 ttatctgccg tgcattaagc cggaaccacg ccgcactatt ggcctggttt atcgtcctgg
    32701 ctcaccgctg cgcagccgct atgagcagct ggcagaggcc atccgagcaa gaatggatgg
    32761 ctatttcgat aaagtgttaa aacaggcggt ttaaaccgtt taacgcagct acccgatagg
    32821 cttccgccat ggtcgggtag ttaaaggtgg tgttgacgaa gtactcaata gtgttgccgc
    32881 cacctttctg ttccataatc gcctgaccga tatgaataat ttcggcagcg cgctcgccaa
    32941 agcagtgaat acccagaatc tcttttgttt cccgatggaa caaaattttc agcgtgccca
    33001 cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa ctgggcgcgg cccacttcat
    33061 atggcacttt catcgcggtc agctgctgtt cggttttgcc cacagagctg atttccggga
    33121 tggtgtaaat gccggtaggg atatcttcaa tcagatgcgc ggtggcttcg ccttttacca
    33181 gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga tgccaggctc ggataaccaa
    33241 tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg atacatgctg ttgaccttca
    33301 gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa cgccagcgaa tcggtattac
    33361 cggtacgacc gttggcatag agcaggcagt cagctttcag ttttttaccc gacttcagat
    33421 gcatgatcac accgtcgtca cagccttcga tcttctcgta ctcttcgttg tgacgaatca
    33481 ctacgccact gttccagaag tgataggaga gagaatctga catctcttga tcgagaaatg
    33541 ccagcaggcg atcgcgggtg ttgatcagat ccacttttac atccataccg cggaagatcg
    33601 acgcatattc acagccgatc actccagcac catagataag tacatggcgc ggttcgtggt
    33661 gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg ggtgaaatca acatctgttg
    33721 gatgatatgg acgagagccg caggcaataa caaatttttc agcggttagt gtttcgacgc
    33781 tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac aaagcgagcg ttcccctgca
    33841 atatttcaca gtgattacgt tcgtaaaatc cctgacgcat gcgcgtttgt tgattaatca
    33901 cgttatcggc atggttaagg atatcggcaa aagaagagcg gagcagtcgc gaatggtcgc
    33961 tgtatagtgg gttttgattg aattctataa tgcggctgac ggcgtgacgg agagctttcg
    34021 acgggatggt gccccagtgg gtgcaaccgc cgccaacatt ttgataacgc tcgataactg
    34081 cgacgcgcac accttgctta accaggccca ttgcagcgcc ttcgccgccg gggccggaac
    34141 ctattactat ggcatcgtaa tcgtaggaat gtggcatggt agggcttacc tgttcttata
    34201 cataaaagca acagaatggt aacattctat cgcgggtaag ccaattgatc cccgtcattt
    34261 atctggctat atcctgagcg gcctttgctt tgtctgtttc ttacttttgc cctgacgttt
    34321 tattggattt ttatcgacga tactctccgt ttaagcggca ggtttccgct gtacgtaaaa
    34381 gaaccggcca gtaaatatgt tttattgcgt taccgttcat tcacaatact ggagcaatcc
    34441 agtatgttca ttctctggta tagtgccagc agtacttttg gcaaggattc agacatcgtg
    34501 atgggcataa gagcgcaaca aaaagaaaaa acccgccgtt cgctggtgga agccgcattt
    34561 agccaattaa gtgctgaacg cagcttcgcc agcctgagtt tgcgtgaagt ggcgcgtgaa
    34621 gcgggcattg ctcccacctc tttttatcgg catttccgcg acgtagacga actgggtctg
    34681 accatggttg atgagagcgg tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt
    34741 atcgccaaag gcgggagtgt gatccgcacc tcggtctcca catttatgga gttcatcggt
    34801 aataatccta acgccttccg gttattattg cgggaacgct ccggcacctc cgctgcgttt
    34861 cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg aacttgcgga ctatctggaa
    34921 ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt
    34981 gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg aacaacgtcg gcaattagaa
    35041 gagcgactgg tactgcaact gcgaatgatt tcgaaagggg cttattactg gtatcgccgt
    35101 gaacaagaga aaaccgcaat tattccggga aatgtgaagg acgagtaatg aaacaagcaa
    35161 atcaagatag aggtacgctg ctgctggcgt tagttgctgg cttatcgatt aatggtactt
    35221 tcgcagcgct gtttagctcc attgtgccat tttctgtatt cccgattatt tccctggtgc
    35281 tgacggttta ctgcctgcat caacgttatc ttaatcgcac tatgccggta ggcttgccgg
    35341 gtctggcagc tgcctgtttt attctcggcg tactgctgta cagcacggta gttcgtgcgg
    35401 aatatccgga tatcggctct aacttcttcc cggcagtact ctccgtcatt atggtgttct
    35461 ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc tgagtaatcg gtattatgcc
    35521 ggatgctggc gcatccggca tgggttttac ttcgcggtca gtaatacgcc gcactccata
    35581 tggtgcgtgt aggggaactg atcaaacaga gccagacgtt cgaccttgtg cgtctggctt
    35641 aatgtttcca gattcttgca taacgtttcc gggttacagg agatgtacaa aatacacgga
    35701 tacgcctgca ccattttctc ggtttcactg tccagaccgc tgcgcggtgg gtcgacaaaa
    35761 atggtttcgc actgataact ctttaagtcg atcccttgca ggcggttaaa ctcgcgcaca
    35821 ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa taatttgtac gttatcaata
    35881 tggttagctg cgatgttgta ttgcgcagca gcaaccgacg gcttagcgat ttcggtggct
    35941 aatacccgat caaaattgcg cgccagcgct aatgaaaagt taccgttgcc gcagtacagc
    36001 tccagtaaat cgccttttga gcctttggtt acgtccagcg cccattccag catctgaata
    36061 ttcatcgccg cgttcggctg ggtaaagctg ttttctacct gacggtagat catctctttc
    36121 cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca gctcgatttt ggttttcgtt
    36181 gcccgaccaa tcagatgcac attcagattc tgcgcgcgca gtgcatcgcg cagggcctcc
    36241 gcttcctgac gccactcatc atccagcttc ttatggtata gcagggaaac caccgcctga
    36301 ttactcagcg tggtgaggta atcaatctgg aacaacttgt ggcgcagaac gggattatta
    36361 cgcacacccg caatcatcgc cgtcatcaac tggttgataa gttcactggc ggcggggaag
    36421 ctatccacgc ggatacggct tttggtttgt tgatcgaaaa tgatgtgata caggtcatcg
    36481 ccatcgtgcc agatgcggaa ctccgcacgc atccggtaat gactgaccgg cgagcgaaac
    36541 acttccggaa ccaggtcaga aaacggtgcc atcatacttt gcaaacgtac cactttttcg
    36601 gctaactgcg cttcatactg ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa
    36661 aaattaaagt acgcggcgat tgtagggatt gctcatcaga tgtccagatc ttgatgagtt
    36721 cctatttgtg agctacgtct ggacagtaac ttgttacaac ctgtagcatc cacttgtcgg
    36781 tcctgtgagt taatagggaa tccagtgcga atctggagct gacgcgcagc ggtaaggaaa
    36841 ggtgcgatga ttgcgttatg cggacactgc catccggtgg gaagtcatca tctcttagta
    36901 tcttagatac ccctccaagc ccgaagacct gccggccaac gtcgcatctg gttctcatca
    36961 tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat tgtggatgct ttacaatgat
    37021 taaaaaagct tcgctgctga cggcgtgttc tgtcacagcc ttttccgctt gggcacagga
    37081 taccagcccg gatactctcg tcgttactgc taaccgtttt gaacagccgc gcagcactgt
    37141 gcttgcacca accaccgttg tgacccgtca ggatatcgac cgctggcagt cgacctcggt
    37201 caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc caaaacggcg gttcaggtca
    37261 gctctcatct atttttattc gcggtacaaa tgccagtcat gtgttggtgt taattgatgg
    37321 cgtacgcctg aatctggcgg gggtgagtgg ttctgccgac cttagccagt tccctattgc
    37381 gcttgtccag cgtgttgaat atatccgtgg gccgcgctcc gctgtttatg gttccgatgc
    37441 aataggcggg gtggtgaata tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc
    37501 agggtgggga agcaatagtt atcagaacta tgatgtctct acgcagcaac aactggggga
    37561 taagacacgg gtaacgctgt tgggcgatta tgcccatact catggttatg atgttgttgc
    37621 ctatggtaat acaggaacgc aagcgcagac agataacgat ggttttttaa gtaaaacgct
    37681 ttatggcgcg ctggagcata actttactga tgcctggagc ggctttgtgc gcggctatgg
    37741 ctatgataac cgtaccaatt atgacgcgta ttattctccc ggttcaccgt tgctcgatac
    37801 ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc tataacggcg aactgattaa
    37861 atcacaactc attaccagct atagccatag caaagattac aactacgatc cccattatgg
    37921 tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa
    37981 caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc gactggcaga aacagactac
    38041 gacgccgggt acaggttatg ttgaggatgg atatgatcaa cgtaataccg gcatctatct
    38101 gaccgggctg caacaagtcg gcgattttac ctttgaaggc gcagcacgca gtgacgataa
    38161 ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc ggttgggaat tcatcgaagg
    38221 ttatcgcttc attgcttcct acgggacatc ttataaggca ccaaatctgg ggcaactgta
    38281 tggcttctac ggaaatccga atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt
    38341 tgaaggctta accgctgggg tgaactggcg tatttccgga tatcgtaacg atgtcagtga
    38401 cttgatcgat tatgatgatc acaccctgaa atattacaac gaagggaaag cgcggattaa
    38461 gggcgtcgag gcgaccgcca attttgatac cggaccactg acgcatactg tgagttatga
    38521 ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg ttacgccgtg ctaaacagca
    38581 ggtgaaatac cagctcgact ggcagttgta tgacttcgac tggggtatta cttatcagta
    38641 tttaggcact cgctatgata aggattactc atcttatcct tatcaaaccg ttaaaatggg
    38701 cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc acctctcacc tgacagttcg
    38761 tggtaaaata gccaacctgt tcgacaaaga ttatgagaca gtctatggct accaaactgc
    38821 aggacgggaa tacaccttgt ctggcagcta caccttctga accacgtccc accgtgctgg
    38881 tgtttgactc cggcgtcggt gggttgtcgg tctatgacga gatccggcat ctcttaccgg
    38941 atctccatta catttatgct ttcgataacg tcgctttccc gtatggcgaa aaaagcgaag
    39001 cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt gcaagaacgt tatccccttg
    39061 cgctggctgt ggtcgcttgc aacactgcca gtaccgtttc acttcctgca ttacgcgaaa
    39121 agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg
    39181 caaatggcat tgtcggatta ctggcaaccc gcggaacagt taaacgttct tatactcatg
    39241 agctgatcgc gcgtttcgct aatgaatgcc agatagaaat gctgggctcg gcagagatgg
    39301 ttgagttggc tgaagcgaag ctacatggcg aagatgtttc tctggatgca ctaaaacgta
    39361 tcctacgccc gtggttaaga atgaaagagc cgccagatac cgttgtattg ggttgcaccc
    39421 atttccctct actacaagaa gaactgttac aagtgctgcc agagggaacc cggctggtgg
    39481 attctggcgc agcgattgct cgccgaacgg cctggttgtt agaacatgaa gccccggatg
    39541 caaaatctgc cgatgcgaat attgcctttt gtatggcaat gacgccagaa gctgaacaat
    39601 tattgcccgt tttacagcgt tacggcttcg aaacgctcga aaaactggca gttttaggct
    39661 gatttggttg aatgttgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga
    39721 ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca
    39781 gcggggttct cctgagaatc tcaacagaga aaagcaaaaa aaatgcttga ctctgtagcg
    39841 ggaaagcgta ttatgcacac c
//
LOCUS       DAZPJP010000017        39408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPJP010000017 DAZPJP010000000
VERSION     DAZPJP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.017"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHU9J_000804"
     CDS             <1..266
                     /locus_tag="ACHU9J_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1904428.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHU9J_000805"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHU9J_000805"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1904429.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHU9J_000806"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHU9J_000806"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1904430.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHU9J_000807"
     CDS             complement(1570..3270)
                     /locus_tag="ACHU9J_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1904431.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHU9J_000808"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHU9J_000808"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1904432.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4339..4797
                     /gene="decR"
                     /locus_tag="ACHU9J_000809"
                     /pseudo
     CDS             4339..4797
                     /gene="decR"
                     /locus_tag="ACHU9J_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4827..6599
                     /locus_tag="ACHU9J_000810"
     CDS             4827..6599
                     /locus_tag="ACHU9J_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1904433.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6592..8373
                     /locus_tag="ACHU9J_000811"
     CDS             6592..8373
                     /locus_tag="ACHU9J_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1904434.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSVLRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8554..8892
                     /gene="glnK"
                     /locus_tag="ACHU9J_000812"
     CDS             8554..8892
                     /gene="glnK"
                     /locus_tag="ACHU9J_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1904435.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8922..10208
                     /gene="amtB"
                     /locus_tag="ACHU9J_000813"
     CDS             8922..10208
                     /gene="amtB"
                     /locus_tag="ACHU9J_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1904436.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10257..11117)
                     /gene="tesB"
                     /locus_tag="ACHU9J_000814"
     CDS             complement(10257..11117)
                     /gene="tesB"
                     /locus_tag="ACHU9J_000814"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1904437.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11335..11907
                     /locus_tag="ACHU9J_000815"
     CDS             11335..11907
                     /locus_tag="ACHU9J_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1904438.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11938..12249)
                     /locus_tag="ACHU9J_000816"
     CDS             complement(11938..12249)
                     /locus_tag="ACHU9J_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1904439.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12412..12508
                     /gene="ffs"
                     /locus_tag="ACHU9J_000817"
     ncRNA           12412..12508
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHU9J_000817"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12628..12981
                     /locus_tag="ACHU9J_000818"
     CDS             12628..12981
                     /locus_tag="ACHU9J_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1904440.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13023..14571)
                     /gene="pdeB"
                     /locus_tag="ACHU9J_000819"
                     /pseudo
     CDS             complement(13023..14571)
                     /gene="pdeB"
                     /locus_tag="ACHU9J_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14735..15205)
                     /locus_tag="ACHU9J_000820"
     CDS             complement(14735..15205)
                     /locus_tag="ACHU9J_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1904441.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15321..15872)
                     /gene="maa"
                     /locus_tag="ACHU9J_000821"
     CDS             complement(15321..15872)
                     /gene="maa"
                     /locus_tag="ACHU9J_000821"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1904442.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16043..16261)
                     /locus_tag="ACHU9J_000822"
     CDS             complement(16043..16261)
                     /locus_tag="ACHU9J_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1904443.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16287..16661)
                     /gene="tomB"
                     /locus_tag="ACHU9J_000823"
     CDS             complement(16287..16661)
                     /gene="tomB"
                     /locus_tag="ACHU9J_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1904444.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17207..20356)
                     /gene="acrB"
                     /locus_tag="ACHU9J_000824"
     CDS             complement(17207..20356)
                     /gene="acrB"
                     /locus_tag="ACHU9J_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1904445.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20379..21572)
                     /gene="acrA"
                     /locus_tag="ACHU9J_000825"
     CDS             complement(20379..21572)
                     /gene="acrA"
                     /locus_tag="ACHU9J_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1904446.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21714..22361
                     /gene="acrR"
                     /locus_tag="ACHU9J_000826"
     CDS             21714..22361
                     /gene="acrR"
                     /locus_tag="ACHU9J_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1904447.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22489..25851
                     /gene="mscK"
                     /locus_tag="ACHU9J_000827"
     CDS             22489..25851
                     /gene="mscK"
                     /locus_tag="ACHU9J_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1904448.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25912..26010
                     /locus_tag="ACHU9J_000828"
                     /pseudo
     CDS             25912..26010
                     /locus_tag="ACHU9J_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26063..26224)
                     /gene="rsmS"
                     /locus_tag="ACHU9J_000829"
     CDS             complement(26063..26224)
                     /gene="rsmS"
                     /locus_tag="ACHU9J_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1904449.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26238..26765)
                     /gene="priC"
                     /locus_tag="ACHU9J_000830"
     CDS             complement(26238..26765)
                     /gene="priC"
                     /locus_tag="ACHU9J_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1904450.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26835..27212
                     /locus_tag="ACHU9J_000831"
     CDS             26835..27212
                     /locus_tag="ACHU9J_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1904451.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27365..27916
                     /gene="apt"
                     /locus_tag="ACHU9J_000832"
     CDS             27365..27916
                     /gene="apt"
                     /locus_tag="ACHU9J_000832"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1904452.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28045..29970
                     /gene="dnaX"
                     /locus_tag="ACHU9J_000833"
     CDS             28045..29970
                     /gene="dnaX"
                     /locus_tag="ACHU9J_000833"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1904453.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29303..29367
                     /gene="dnaX"
                     /locus_tag="ACHU9J_000833"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30023..30352
                     /locus_tag="ACHU9J_000834"
     CDS             30023..30352
                     /locus_tag="ACHU9J_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1904454.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30352..30957
                     /gene="recR"
                     /locus_tag="ACHU9J_000835"
     CDS             30352..30957
                     /gene="recR"
                     /locus_tag="ACHU9J_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1904455.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31067..32941
                     /gene="htpG"
                     /locus_tag="ACHU9J_000836"
     CDS             31067..32941
                     /gene="htpG"
                     /locus_tag="ACHU9J_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1904456.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33122..33766
                     /gene="adk"
                     /locus_tag="ACHU9J_000837"
     CDS             33122..33766
                     /gene="adk"
                     /locus_tag="ACHU9J_000837"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1904457.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33898..34860
                     /gene="hemH"
                     /locus_tag="ACHU9J_000838"
     CDS             33898..34860
                     /gene="hemH"
                     /locus_tag="ACHU9J_000838"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1904458.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34857..35816)
                     /gene="aes"
                     /locus_tag="ACHU9J_000839"
     CDS             complement(34857..35816)
                     /gene="aes"
                     /locus_tag="ACHU9J_000839"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1904459.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35968..37272
                     /gene="gsk"
                     /locus_tag="ACHU9J_000840"
     CDS             35968..37272
                     /gene="gsk"
                     /locus_tag="ACHU9J_000840"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1904460.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37405..39081)
                     /gene="ybaL"
                     /locus_tag="ACHU9J_000841"
     CDS             complement(37405..39081)
                     /gene="ybaL"
                     /locus_tag="ACHU9J_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1904461.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39319..>39408)
                     /locus_tag="ACHU9J_000842"
     CDS             complement(39319..>39408)
                     /locus_tag="ACHU9J_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904462.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9525 a        10049 c        10419 g         9415 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 attctccgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
     4381 caggattgca ccctctcttt gtaggcactg gctgaagccg ttaatctgac aaccacccct
     4441 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
     4501 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
     4561 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
     4621 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
     4681 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
     4741 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
     4801 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
     4861 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
     4921 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
     4981 ctaccgggct gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
     5041 tcctgcgtta tgtctggcgc gtattgctgt ttggcgcgtc ttaccaactg gctgttgaac
     5101 tgcgtgaaga ttattaccgt cagctaagcc ggcagcaccc ggagttttac ctgcgtcatc
     5161 gcacgggtga tctcatggct cgtgcgacca atgacgtcga ttgcgtcgtg tttgccgccg
     5221 gagaaggggt gctgacgctg gtggattcac tggtaatggg ctgcgctgtg ttgattatga
     5281 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
     5341 cgatcatgat taagcgcaac ggcgatgctt tgcatgaacg ctttaagctg gcacaggcgg
     5401 cgttttccag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
     5461 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
     5521 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
     5581 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
     5641 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
     5701 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
     5761 gtgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
     5821 gtggcgaact tgatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
     5881 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
     5941 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtt agcgaggggg
     6001 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
     6061 tggcggtggt cagccagacg ccattccttt tttctgacac cgtggcaaat aacatcgcgc
     6121 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
     6181 atgacgatat tttgcgttta ccgcaaggtt acgatacaga ggtgggcgag cgcggcgtga
     6241 tgctttccgg cgggcaaaaa cagcgtatct ctattgctcg tgcgttatta gtcaacgcgg
     6301 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
     6361 tgcataacct gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt
     6421 cggcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
     6481 gcaatcatga tgtgctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
     6541 aactggaggc ggcgctcgac gacgctccgg aaaatcacga ggaggccgtc gatgcgtagt
     6601 tttagccaac tgtggccgac tctcaagcgc ctgttagcgt acggttcacc gtggcgtaaa
     6661 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcgg cggcagaagt cagtgggccg
     6721 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaagtg
     6781 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
     6841 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
     6901 gtgatggatg ctgcgttacg ccagccatta agcgagtttg atacccaacc cgtcgggcag
     6961 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
     7021 gcaactgtcc tgcgcagtgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
     7081 gactggcgaa tggcgctggt ggcgataatg attttcccgg tagtgctggt ggtaatggtg
     7141 atatatcaac gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
     7201 aacgacggct ttaacgaaat catcaatggc atgagcgtca tccagcagtt ccgtcagcag
     7261 gcgcgatttg gcgaacgtat gggtgaggcc agtcgttcac actatatggc gaggatgcaa
     7321 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
     7381 ctttgtggat tgttgatgct gtttggcttc tccgccagcg gcaccattga agtgggcgtg
     7441 ctgtatgcct ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
     7501 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
     7561 ggaccgcgcc agcaatatgg caatgatgat tacccgttac agagtggcac catcgaagtc
     7621 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
     7681 gtgccttcga gtaactttgt ggcgctggtc gggcataccg gcagtggtaa aagtaccctc
     7741 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
     7801 ccattaagtt cgctaagtca cagcgtgctg cgccagggcg tggcaatggt gcagcaagat
     7861 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcggga tatctccgaa
     7921 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg tagcatgagc
     7981 gacggtattt acacgccgct gggcgagcag gggaataatc tctcagttgg gcaaaagcaa
     8041 ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa tcctgatcct tgatgaggca
     8101 accgccagca ttgactccgg gactgaacag gcgattcaac atgctctggc ggcggtgcgt
     8161 gaacatacca cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc
     8221 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggct
     8281 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
     8341 agcgtgcgtg aagaggaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
     8401 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaaactt cttaacttcc
     8461 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
     8521 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
     8581 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
     8641 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
     8701 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
     8761 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
     8821 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
     8881 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
     8941 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
     9001 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
     9061 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
     9121 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
     9181 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa
     9241 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
     9301 gctcgtttgc ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct
     9361 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
     9421 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
     9481 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
     9541 gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta
     9601 ctgccattct ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
     9661 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
     9721 ggatcttcgg tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg
     9781 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt
     9841 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct
     9901 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
     9961 gctgtatcat gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag
    10021 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    10081 ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg
    10141 tgccggaaga acaggagcga gaagggctgg acgtcaacag ccacggcgag aatgcctata
    10201 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    10261 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagcacgcc gtcctgagta
    10321 taaaactcac cgcgcacaaa gccgcgtgcg ctggacgccg aggtgctctc cacgctatac
    10381 agcagccatt cattcaaatt aaacgggcga tgaaaccaca tggaatggtc aatggtggca
    10441 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    10501 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    10561 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    10621 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttcagcact
    10681 ggcggcagca ggtgcgccag cgattgggcg atttgtgttt ccgaaggcag gccatcaggc
    10741 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttcaggtgc ctggaaagag
    10801 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    10861 ctgttaccgt cacgcagcgt ttcgacatca taaataatcg gcttcttact atcgccaggg
    10921 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt cagggacggt ctcttttgca
    10981 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    11041 ctctggccgc gaaagagtcc ttcctcaatt ttttccagat ttaacaatgt cagtaaattt
    11101 tttagcgcct gactcatata actctccagt aactaagctg ccgcagcaag ccaaagtgag
    11161 ttgagtataa cgcaaatttg ccactggtcc gatgggtgca atagtctgaa ttacgggcta
    11221 attacaggca gaaatgcgtg atgtgtgcca cacttgttga cgttactatt ttgttaacca
    11281 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa gtgaatgaaa
    11341 ctcgtgcaca tggccagttg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    11401 agcgcggata ttcagacgcc agcaccggcg gcaaatacgt ctatttcagc aacacaacaa
    11461 cctgctatcc agcaaccgaa tgtctccggt accgtttgga tccgtcagaa agtcgcactg
    11521 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcgccgtca
    11581 aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt
    11641 ctgcctttta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    11701 accgtgaatg acaaactggt atttattacc gataccgttc agccggtgat caacaagggc
    11761 ggaactaaag ccgacctgac attggtgccg atgcagcaaa ccgccgtgcc ggttcaggcc
    11821 agcggtggtg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    11881 gcagttccag ctcctacgca atattaagcg acgttaaccc tctccgcccg gagagggtta
    11941 gtagttccag cgataacgct gcaagtcgat ttgcccgctt cccgatacca tcacaccttc
    12001 tgccagtaat gcctgtcgct gacgctgtaa atccggtccg gttagcgaaa ttgtgccgtg
    12061 gcgattaacc acccggtgcc agggtaaggt gctgccttca gggagacgct ttaacacacc
    12121 gcccacctgg cgcgcggcgc ggggcgatcc cgccagtttc gccacatcac cgtaagtggt
    12181 gacatagcct tcgggaatag cggcgacgat ttgccagacg cgttggggaa atgaatcttc
    12241 tttttccatc ttttcttcct gaggtaattt ttcagcataa tctggaaaaa cgcccgagtg
    12301 aagtcgcatt gcgcaagaaa ccagcatctg gcacgcgatg ggttgcaatt agccggggca
    12361 gcagtgataa tgcgcctgcg cgttggttct caacgctctc aatgggggct ctgttggttc
    12421 tcccgcaacg ctactctgtt taccaggtca ggtccggaag gaagcagcca aggcagatga
    12481 cgcgtgtgcc gggatgtagc tggcagggcc cccacccatt tctgcctccc accgtttcgt
    12541 caaaaaatcc caacatagct aaactttaac cacaactgac gtcgcaagaa ttgtctggct
    12601 gcgcagtacg cttcggaggt atgtctgatg aagtatgttg atggttttgt ggttgccgtt
    12661 cctgccgata aaaaggatgc ctatcgggaa atggccgcta aggctgcgcc attgtttaaa
    12721 gagtttggcg cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg
    12781 accgattttc gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt
    12841 gaataccctt caaaagaggt ccgcgacgct gctaatcaaa agatgatgtc ggacccacgg
    12901 atgaaagagt tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggcggattc
    12961 gagtcaatca tcgacgaata ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa
    13021 attcacaaat gttgctcggc ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag
    13081 agccagccct gaccgtagtg cacgccatgc tggcgtagcc actcttcttg tttactggtt
    13141 tcgattccct ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc
    13201 gtgacatttt tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca
    13261 tccagatcct gtaaataact taaacttgaa tatcccgtac caaaatcatc aagataaatt
    13321 tcatggcccg cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac
    13381 tcgcgttcag tgagttcaag cgcgatctgt ctgggattaa cctgattgat tgatcatttc
    13441 acgcagcaat tgcgggattt tttccgaggt gagcacggtg gattcaagat tgatcgaaat
    13501 atgctgctgt ggatgctgac gcagccagtc gcccatatct tcaaaggcgc ttcttataat
    13561 cagtagcgtc aatggctcag aaaggcccgt ttgctgtgcc agcggaataa aactatctgg
    13621 tgacaaccaa ctaccgtctg tctgcggcca gcgcgccagt gcctcagcac cgacaatttt
    13681 gccattggct aaggagacaa tcggctgata gtgcacgcaa atatcacgat tttcgatagc
    13741 atcctgcagc cgatgatgcg gtgactggat acggcgcaaa atacgcagca caaacatcgc
    13801 tgccagcagg ccaatcacca acccgagcgg taaccaaata aagacttgcc gatgccagcc
    13861 tttctgcaac attttcgttg aagcccaggt gatgatcgaa atattcatct ccgggaaggg
    13921 caggatatcg taaatgattc cattattttc gatatgctca ccgggtgttt tttgtagcct
    13981 ggtaataatt ccctgagcaa tttcatcgct gctggttatg acaacgttat gggcattgcc
    14041 aataatggcg gcatcaattt gccatgagct atagggaatg acatcaataa aggaagcggg
    14101 gtcgatcatg acgacataat gtgccgttcc catggcgacc atgtaacgga taatgcctaa
    14161 atcgttatgc gatgttaacc agacacgata accatctttc gaaattttac ctggctcggg
    14221 gaaggtatcg ggcggacttt catgctccag agacgaacac tggggaacgt tgttatcgat
    14281 ataaaccact tcctgaatat agcgataact gtaagatacc cgacgcattt ccatgagatg
    14341 ggcttcgcta caggcagcgc cttgccatct ttccagctct tgcagcgcat ctttcccttg
    14401 tgtcgccacc ttattggccc gaatagcgac gcgggaggaa taggtatcca gctcttcaat
    14461 aaacgatgtt tctacctgct gatgggccag ccagatgctt aagccgacag gcagcaatac
    14521 tgaaagaatc agtactcccg aaataaggcc gaccagatgt cgtgttctca ccagcatatc
    14581 cttattaaca cagtgtaggg aaagtatatc tgattgtgga aaaaggctgg caggagataa
    14641 ggaggtagag ctggtatttc atgaagtgat gggtggaaac gatcattgtc atttgaccaa
    14701 gcgcagagct ttacgcctgg tatgtaaccc taacttaaga tgttgattcg gcgcgagcga
    14761 gggtaaaaat atcgtaaaaa gacagttcac ctttacggac gatcattttt tgcagttgtt
    14821 ccttatgaac atccgcgaca cgcggagagt tcaagatggc ttcaaccagc gcggcaggta
    14881 caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg gaaatccagc acgtcgatat
    14941 cttcatagcg gtagcgtggg tagacatcga aaaagaagaa accattaagc aggataaata
    15001 atacaaccag tagccagaca gagcctgaca gcgtttcgga ctgcaacatc accgccaggg
    15061 tggcaaaaaa agcaacgtac ataccgataa acagccccgg atgtttacgg ataaaactga
    15121 tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt cagagactca atcgtttcgg
    15181 tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg tatctgcaac tttccaggga
    15241 acaccctatt ttaacgtggg aatagtagag gggaagtaac agatttacca caattttgca
    15301 taacagttgc gaaaaaccga ttacaatttt tttattattc tggctggatt accgcccacg
    15361 acaacgttgt ccgggacatc ttttgtgaca actgcacctg aggctaccac gacgttatca
    15421 ccaatggtca caccagggtt aatgaccgcg cgtccgccaa tccagacgtt attaccgatg
    15481 gtgacgggtt tccccagttc agcaccgcta ttacgtgcta cagggtcgat gggatgtgtt
    15541 gccgtgtaga tatgaacgcc tggtgccaac atacagttat caccgatgcg aatagggcag
    15601 acatcaagca tcacgcaatc gaagttggcg aaaaaattat taccgagaaa aatgttatag
    15661 ccatagtcac agcgaaacgt tggctcaata taagcctctg tcacctgacc gaatagatca
    15721 gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg aatgattgta tcggtgaata
    15781 agctgacgag cgcgcaggcg atcgcgagat aacgtctcat ctgccgagcg atacaactca
    15841 ccagcaatca tcttttcttt ttctgtgctc attactgacc tccggttgct gaaaatgcgg
    15901 caacacagta agacgaaaag tcagtgaaga gaacgttccc attacatctg tgtgataaag
    15961 atcacatagg gtatgtcttc atggtgaaaa gtataaaatt cttaataaac agccggttat
    16021 agctccgaaa gcgaattatt tattagcgaa taaatttcca tactgaggaa gggatcttgt
    16081 cgtatagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag taaaataccg
    16141 ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc agcgtgtcaa
    16201 ttgtctggca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt ttttcggaca
    16261 tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt ctctttcgtc
    16321 gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg aagatcctgc
    16381 atattaatac cataactact gaacaacata aaggtgtcat ccagatattc gtcgatctgc
    16441 tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa ggtcgcaata
    16501 tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt aacccagcca
    16561 tgattgcttt cttcaaggtt tgcaaggcag tcatgataca gggtttcaca gagaaactta
    16621 agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc ccttcttagc
    16681 ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga cttgccctct
    16741 cgtttgttag cttaactata agccactctt tgcaggtttt catcgcatta ttacgaaaaa
    16801 ttacaattag gaaggaatcg ggacaggact cactttttca tactagcact aacccgcagc
    16861 aggtaaaagc agtctgctcg aactgttcat aacatttcag gattatcgcc agatgaaatt
    16921 ggtgacacta ataccaggat tgctctgaat atgacgtaat aaccgaggaa tgaataaaga
    16981 attaccgcaa ataattaaga atagcctcta aatgattatg gattattaaa tctattagcg
    17041 ctacttaata taaattaact aacaatcagt attttttatg aattttatcc gtggttaata
    17101 ctggttttcg tatgagatcc tgagttggtg gttcaattac tccttaatgt tcgtaggtta
    17161 tgcataaaaa aggccgcttg cgcggcctta gtgattacac gttgtatcaa tgatgatcga
    17221 cagtatggct gtgctcgata tcttcattct tgcggctaaa gcggcggcga accaccacaa
    17281 agaataccgg aacgaagaag attgccagta ccgttgcggt caccatcccg cccattacac
    17341 cggtacctac tgcgttctgc gcgccggaac cagcaccagt actgataacc agcggcataa
    17401 cgccgaggat aaacgccagc gaggtcatca ggatcggacg taaacgcatc cgcactgcat
    17461 caagcgtcgc ttcaatcaga cctttacctt ctttatccat caagtctttg gcgaattcga
    17521 cgataagtat cgcgttcttc gccgacaacc caatggttgt gagcaggcct acctggaagt
    17581 aaacgtcatt ggtcaggcca cggaaggtgg cagccagcaa cgcaccgata acccccagcg
    17641 gaacgaccag cataacggag aacggaatcg accagctctc gtacagcgcc gccagacaca
    17701 ggaatacgac aatcaacgaa atcgcgtaca gtgaaggtgc ctggttgccg gagagacgtt
    17761 cctgatagga catccccgtc cagtcatagc caacaccggt aggcagtttg ctcgccagtt
    17821 gttccatcag ctccattgct tcaccggtac ttttacccgg tgccgcctgg cctaagattt
    17881 ccatggatgg caggccgttg taacgttcca gacgcggcga accgtactcc caacgagaag
    17941 aggagaacgc cgagaatggc accatctgac catcagcagc acgaacatac cagtcgccga
    18001 tatcatccgg cagcatacgg tatttcgctt ctgacatgac gtaaactttc ttcacacgac
    18061 cgcggtcgat aaagtcgttc acatagctac cgccccatgc agcgcccaga gaggtgttaa
    18121 tgtcgttgat agaaacaccc agcgcctgcg ctttttcctg gtcgatatca atcttaaact
    18181 gcggggtatc ttccagaccg tttggacgta cgctggtcag catatcaggg tgcttcgctg
    18241 cttctgcaag cagctggtta cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt
    18301 caatcagctc aaagtcaaag ccggttgcag tacccagttc cacgattgcg ggcaggttaa
    18361 aggcgaaaac catcgcatct ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg
    18421 cttcaacttt gttttcttcg cccggacgat cggcccagtc cttcaaggaa acgaacgcaa
    18481 taccggtatt ctgaccacgt cccgcaaagc cgaagccgtt aacggcgaac accgactcaa
    18541 cgttgttctt ttctttggtc agatagtaat gcgttacctc attgagcact ttctgcgtac
    18601 gttcctgcgt tgcacctgct ggcagctgaa ccatggtcat aaatacgccc tggtcctcat
    18661 ctggcaagaa ggagcttggc agacgcacga acagataggc catgccgacc acgatgatca
    18721 gatacagcac caggtaacgc cccgtactgc gcagaatacc gcctacgctg tcggtgtagt
    18781 ggtgcgtgct cttctcgaac atgcggttaa accagccgaa gaagcctttt ttaccttccc
    18841 cgtgatcgcc tttggcaatc ggtttcagca tggtggcaca aagagccgga gtcaggatca
    18901 acgctaccag taccgacagc gccattgctg aaacaatggt aatagagaac tgacgataga
    18961 tagcaccagt agaaccgcca aagaaggcca tcggtacgaa taccgccgac agtaccatcg
    19021 cgataccgac cagagcgccc tgaatctgcc ccatcgactt acgggtagct tcttttggcg
    19081 gcaaaccttc ttccgccata acacgctcca cgttttctac cacaacgata gcgtcatcca
    19141 ccaacaagcc gatggcgagc accatcccga acattgttag cgtgtttatc gagaagccaa
    19201 aggcggcaag gacggcaaag gtcccgagca ataccaccgg tacggcaatg gtcggaatca
    19261 acgtcgcgcg gaagttctgc aggaacagat acataaccag gaacacgagg atgatcgctt
    19321 cgaccagcgt tttaaccact tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg
    19381 ggtaaacaat tttcagaccc gacgggaaga acggttccat cttcgccagt tcagcacgga
    19441 ttgccgcagc ggtatccagc gcgtttgcac cggtcgccag cttgatcccc agaccggaag
    19501 ccggttggcc gttaaactct gcgatgatgt cgtagttctc accacccagc tcaatcttcg
    19561 ccacatcacg cagcagtacg cgggaaccat cctgattcac tttcagcagg attttgccga
    19621 actcttcagt agaggtcaga cgcgtctgag caataataga ggcgttaagc tgttggcctt
    19681 tcaccggcgg cgtaccaccg agctgacccg ccgcaacctg ggcgttctgc gctttgatgg
    19741 cggtaatgac atcaaccggc gttagctgga atttgttcag ctcattcggg ttcatccaga
    19801 tacgcatcgc gtactgtgaa ccgaacaact gaacatcacc cacgcccgac gtacggctga
    19861 tggcatcttt catattcgcc gctacgtagt cagagatatc ctcctgcgtc atggtgccat
    19921 cggtgttgat aacgccgaca accatcagga agctgctgga tgatttctca acgctcaccc
    19981 cttgctgctg aacttcttgc ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct
    20041 gaacctgcgc gatatccgca tcagtaccag actcaaaggt cagggtgatc tgcacggtac
    20101 ccgtggagtc actgttagag gacatgtaca tcaggttatc gataccgttc atattctgtt
    20161 cgataacctg tgtcaccgtg tcctgcactg ttttcgcatc agcgccgggg taggaggcgg
    20221 agatcgttac tgccggcggt gcaatcgtag gatattgcgc caccggcagt ttgaggatcg
    20281 ccagcccccc tgccaacatg atgataatgg cgatcaccca cgcaaaaatc gggcgatcga
    20341 taaagaaatt aggcatgtct taacggctcc tgtttaagtt aagacttgga ctgttcaggc
    20401 tgagcaccgc ttgcggcttg ctggttatta tcagcggtaa cttcttgtgc ttttacctgg
    20461 acaccaggac gcactttctg cagcccactt attactacgc gatcgcctgc tttcagacct
    20521 tctgtcacca gccacttatc gccaatagcc tggcttgcaa cgatcggacg ggtttccact
    20581 ttgtcatccg cgccaactac cagtacggtg gcatcaccac gcggcgtacg ggttacgccc
    20641 tgttgcggga ctaaaatagc gtttggatta agcccttctt ccagacgtgc gcgcacgaac
    20701 atacccggca gcagagtgtg atccgggttc gggaagatag cgcgtagggt gatagaccca
    20761 gtggtctgat caacggtaac gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg
    20821 ccgtcactgg tgatcagcga cactttggct ttgccgttct cttgtttcag cgtgccattc
    20881 gccagttcct gtttcaggcg caggaagtcg ttgctggact gggtcacatc aaggtagatc
    20941 ggatcaagtt gctgcacggt tgccagcgca gtcgcctgac cgttctgtac caatgcgcct
    21001 tccgtcacgt tcgacttacc aatgcgaccg ctaatcggag aggtgacttt ggtgtaagcc
    21061 agattgatcc gcgcagtttc aacggcagct ttcgccgcag ttaccgcagc attcgcctgt
    21121 tgcgcatcag ccagagcctg atcgtactct tgcttactga tgtactgagt accgagcagt
    21181 ttctgataac gattcaccgt caattgcgcg atattggctg cagcctgggc tttcgccaga
    21241 tcacctttcg cactgtcgta tgtcgcctga taggtcgcag gatcaatctg atagagagag
    21301 acaccagctt cgatgtcgct accttctttg aaattacgct tcaggataat cccgctaact
    21361 tgaggacgaa cttctgcgat ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc
    21421 tgcagaggtt cagttttgac tgttactacg ccaacggcgg gcatctgctg gccaccttgt
    21481 tgggcctgtt tgtcgtcaca tcctgttagg gctaagctgc ctgagagcat cagaacgacc
    21541 gccagaggcg taaaccctct gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa
    21601 ttggtcaatg gtcaaaagtt aataaaccca ttgctgcgtt tatattatcg tcgtgctatg
    21661 gtacatacat tcacaaatgt atgtaaatct aacgcctgta aattcacgaa catatggcac
    21721 gaaaaaccaa acaagaagcg caagaaacgc gccaacacat cctcgatgtg gctctacgtc
    21781 ttttctcaca gcagggggta tcatccacct cgctgggcga gattgcaaaa gcagctggcg
    21841 ttacgcgcgg tgcaatctac tggcatttta aagacaagtc ggatttgttc agtgagatct
    21901 gggaactgtc agaatccaat attggtgaac tagagcttga gtatcaggca aaattccctg
    21961 gcgatccact ctcagtatta agagagatat taattcatgt tcttgaatcc acggtgacag
    22021 aagaacgacg tcgattattg atggagatta tattccacaa atgcgaattt gtcggagaaa
    22081 tggctgttgt gcaacaggca caacgtaatc tctgtctgga aagttatgac cgtatagaac
    22141 aaacgttaaa acattgtatt gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg
    22201 cagcaattat tatgcgcggc tatatttccg gcctgatgga aaactggctc tttgccccgc
    22261 aatcttttga tcttaaaaaa gaagcccgcg attacgtcgc catcttactg gagatgtatc
    22321 tcctgtgccc cacgcttcgt aatcctgcca ctaacgaata accctgaatc tgactccagg
    22381 atttttcctg gacattttcg tcgttgctat tctggttcac tgcgtcgtga tattcttgcg
    22441 gtttgacttt ttcaggtcgt tcttcaggtt cagaaacctt cattcatcat gactatgttc
    22501 cagtattaca aacgatcacg gcattttgtt ttttcagcat ttattgcttt tgtttttgtc
    22561 ttgttatgcc agaacacggc gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa
    22621 gcggacctgc aggcgcaact tgactcacta aataaacaaa aagatctttc tgctcaggac
    22681 aaactggtgc agcaggatct gacagataca ttagccaccc tcgataaaat cgatcgcata
    22741 aaagaagaga cagttcagct acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag
    22801 gcgaccgcgg cgttaacagc acttagcgat gtcgataacg acgaagaaac gcgcaaaatt
    22861 ctgagtacgc tttcgttgcg ccagctggaa actcgcgttg cccaggcgct ggacgatttg
    22921 caaaacgcac aaaacgatct ggcgtcttat aacagccagc tggtttcgtt acagacgcag
    22981 cccgaacgcg tgcaaaatgc gatgtataac gcttcgcagc agctgcaaca aattcgcagt
    23041 cgtctggatg ggactgatgt cggcgagact gccttacgtc ccagccagaa agtgttaatg
    23101 caggctcagc aggcgttgct gaatgcggag attgaccagc agcgtaaaag cctggaaggg
    23161 aacaccgtct tgcaggatac cttgcaaaag caacgtgatt acgtgacggc gaacagcgcc
    23221 cgtctggagc accagttgca gctgttgcaa gaagcggtaa acagcaagcg cctgacttta
    23281 actgaaaaaa cggcgcagga agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat
    23341 ccgctggtga aacaggaact ggaaattaac cagcagttaa gtcagcgtct gattaccgcg
    23401 actgaaaacg gtaatcagtt gatgcagcaa aacattaaag tcaaaaactg gctggagcgg
    23461 gcgctgcaat cggaacgcaa tattaaagag cagattgccg tcctgaaggg cagcctgctg
    23521 ttgtctcgta tcctttacca gcaacaacaa acgctgccct cggcggatga actggaaaac
    23581 atgaccaacc gcatcgcgga tttgcgtctc gaacagtttg aagttaacca gcagcgtgat
    23641 gcactcttcc agagcgatgc gttcgtcaac aaactggaag aaggtcacac caacgaagtc
    23701 aacagcgaag ttcacgatgc gttattgcaa gtggttgata tgcgtcgcga attgctggat
    23761 caactcaaca aacagttggg taaccagctg atgatggcca ttaacctgca aatcaaccag
    23821 cagcagttaa tgagcgtatc gaaaaacctg aaatccatcc tgactcagca aatcttttgg
    23881 gtgaacagta accgtccaat ggactgggac tggatcaaag cgttcccgca aagcctgaaa
    23941 gatgaattta agtcgatgaa aatcacggtg aactgggaaa aagcctggcc tgccgttttt
    24001 atcgctttcc tcgccggttt gccgctgctg ttgattgccg ggctgatcca ctggcgtctg
    24061 ggctggctga aagcgtatca acagaaactg gcttccgctg tgggttccct gcgtaacgat
    24121 agccagctca acacaccaaa agcgatcctt atcgacctga tccgcgcgct gccggtgtgc
    24181 ctgattattc tcgcggttgg cctgattctg ttgaccatgc agctcaacat cagcgaactg
    24241 ctatggtcgt tcagtaaaaa actggcgatt ttctggctgg tgtttggcct gtgctggaag
    24301 gtactggaga aaaatggcgt tgccgtgcgt cacttcggca tgccggagca gcagaccagc
    24361 cactggcgtc ggcaaattgt ccgcatcagt ctcgcgttgc tgcctatcca tttctggtct
    24421 gtggtggcag aactttcccc gctgcatctg atggatgatg tgctggggca ggcgatgatt
    24481 ttcttcaacc tgctgctgat tgccttcctg gtatggccaa tgtgccgcga aagctggcgt
    24541 gataaagagt cgcacaccat gcgactggtc accattaccg tgctgtcgat tatcccgatt
    24601 gcattgatgg tgctgactgc cacgggttat ttctacacca cgctgcgcct ctccgggcgt
    24661 tggattgaaa ccgtttatct ggtgatcatc tggaacttgc tctaccagac ggtattgcgc
    24721 ggcttaagcg tagcggcgcg gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat
    24781 ctggtgaaag agggcgcaga aggtgccgaa ccgccggaag aacccaccat tgcactggaa
    24841 caggttaacc agcagacgct gcgtattacc atgttgctga tgtttgcgct gttcggtgtc
    24901 atgttctggg caatttggtc cgatttgatc accgtgttca gctatctcga cagcatcacg
    24961 ctctggcatt acaacggcac tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc
    25021 agtctgttgt ttgcgattat cgcctcaatg gtggcctggg cgttgattcg caacctgcct
    25081 ggtttactgg aagtgctggt gctctcgcga ctgaatatgc gtcagggcgc gtcgtatgcc
    25141 attactacca tccttaacta catcattatt gccgttggtg cgatgacggt gttcggatcg
    25201 ctgggcgtct cttgggataa actccagtgg ctggccgcag cattatccgt aggtcttggt
    25261 tttggtttac aagaaatttt cggtaacttc gtctccggtt tgatcattct gttcgaacgt
    25321 ccggtgcgta ttggcgatac ggtaaccatt ggtagcttct cggggacggt aagtaagatc
    25381 cgtattcgtg cgacaacgat taccgatttc gatcgcaaag aagtgatcat cccgaacaaa
    25441 gcgtttgtta ccgagcgtct gatcaactgg tcgttgactg acactactac gcgtctggtg
    25501 atccgtctcg gcgtggccta tggctccgat ctggaaaaag tgcgtaaagt gttactgaag
    25561 gcggcgactg agcacccaag ggtgatgcac gaaccaatgc cggaagtctt ctttacggcg
    25621 tttggtgcca gcacgttgga tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt
    25681 agtcgtactg tcgatgagct gaaccgtact atcgatcagc tgtgccgtga aaacgacatc
    25741 aacattgcct ttaaccagct tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg
    25801 gaagtcaaac gcgactacaa aggcgatgac ccgacgccag cggtagggta ataaccaaag
    25861 ggggcgacat ttagttgccc cgagattact gacaaagtgc gcgttgttca tgccggatgc
    25921 ggcgtgaccg ccttatccgg cctacgaaac cgcaagaatt caatgtattg caggagcggt
    25981 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgcccacaat cttaggggac
    26041 atttagcggc cccatttatt tctcactttt ccgcctcatc atcgcgcgtt aatttctttt
    26101 cataatcgcg ttttacaata tccagcgcgc gcagcacggt gcgggctggg atctgatttt
    26161 cttccagcag cacaatcaaa tcgacagcca gtttgacatc gtcaggggca ttttcaagtg
    26221 acatactctc tccattgcta gcgggttaag cgcgctagcc tgttttcgat tttttccagc
    26281 gcatggcggc agcgtgccag gcgcgcttca taggcttcca cttcacgatg cagcgtttgc
    26341 tgttccacga gatcggtcac ccgcgccagg cgggctctgc gttcggcaac catctcacgt
    26401 agccgccgct caaaatcctg atgctgaata cgtttacgct gccagcgggc aattttcggt
    26461 ggcgcactgt cccactcgcg caatgaccag gcggaggctt cacgcgcgat ggcttccagt
    26521 tgtgccgcca gatgttccgc cagccaggcc acttgcggca gctgttgctg ctcaaccgca
    26581 tgacaaagcg cagccagatt atcgcccgcc tcgtcgagac aagcctgtag tgttgtcgca
    26641 cgagtctgaa aaagatgcct gtcgaaacga gcacttagcg tggcgaactg tgacaccggg
    26701 gcacaacgct gacgcagcgt agcgagctgt ccttccagtt tttccagcag cagggcggtt
    26761 ttcacgataa gacctcaatg aaaatgataa ttgttatgct aaagtagcca ctctgtaagc
    26821 tgacaacact caatatgcaa cgaatcattt taatcatcat tggctggctg gcggtagtgc
    26881 tgggtacgct gggcgtggta ttaccggtat taccgacgac gccgtttatc ctgctggcgg
    26941 cctggtgctt tgcccgttct tccccgcgct ttcacgcctg gttgctgtac cgctcatggt
    27001 ttggcagcta tctacgtttc tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc
    27061 gggcgatttt gcttattttg ctcacgtttg ccatttctct gtggttcgtc cagatgccat
    27121 gggtgcgcat catgttgctg gtaattctcg cctgtttgct tttctatatg tggcgaattc
    27181 cggtgattga tgaaaagcaa gaaaagcact gaagcacaac aatcgcagtt gcaattattg
    27241 cgtacagcca gtacattctg gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca
    27301 actgttactt ttgatacgtt taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac
    27361 acttatgacc gcgactgcac agcagcttga gtatctcaaa aatagcatca aaagcattca
    27421 ggactaccca aaacccggca ttcttttccg cgatgtcacc agcttactgg aagacccgaa
    27481 agcttacgct ctcagcatcg acttgctggt tgagcgttac aaaaatgcgg gcattaccaa
    27541 agttgtcggc accgaagcgc gtggcttctt gtttggcgct ccggtagctc tgggtctggg
    27601 cgttggcttt gtaccggtcc gtaaaccggg caaactgccg cgtgaaacca tcagtgaaac
    27661 ttacgacctg gaatacggca ccgatcagct ggagatccac gttgatgcca tcaaaccggg
    27721 cgacaaagtt ctggtggtgg acgatctgct ggcaaccggc ggcactatcg aagcgaccgt
    27781 taaactgatc cgtcgtctgg gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt
    27841 cgatctcggc ggcgaacagc gtctcgaaaa acagggcatt accagctaca gccttgtccc
    27901 gttcccgggc cattaattat cgccagtctt gtgctgccca cgctacggac agcacaagat
    27961 gtgcattcag cctcgccgtt ctgacggggc tgtgttagca ttaccccttc gtgaatccac
    28021 cttccagcgt ttcagagcct gccaatgagt tatcaggtct tagcccgaaa atggcgccca
    28081 caaacctttg ctgacgtcgt cggccaggaa catgtgctga ccgcactggc gaacggcttg
    28141 tcgttagggc gtattcatca tgcttatctt ttttccggca cccgtggcgt cggaaaaacc
    28201 tctatcgccc gactgctggc gaaggggcta aactgcgaaa ccggcattac cgcgacgccg
    28261 tgcggcgtgt gcgataactg tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa
    28321 atcgacgccg cctcgcgcac caaagttgaa gatacccgcg acctgctgga taacgtccag
    28381 tacgctccgg cgcgtggtcg tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg
    28441 cgccacagct ttaacgcact gttaaaaacc cttgaagagc cgccggagca cgttaagttt
    28501 ctgctggcga cgaccgatcc acagaaattg ccggtgacga ttttgtcgcg ctgtttgcaa
    28561 tttcatctca aggcgctgga tgtcgagcaa attcgccatc aacttgagca catcctcaac
    28621 gaagaacata tcgctcacga gccgcgggcg ttgcaattgc tggcgcgcgc cgctgaaggc
    28681 agcctgcgag atgccttaag tctgaccgac caggcgattg ccagcggtga cggccaggtt
    28741 tcaacccagg cagtcagtgc gatgctgggt acgcttgatg acgatcaggc gctgtcgctg
    28801 gttgaagcga tggtcgaggc caacggcgag cgcgtaatgg cgctgattaa tgaagccgct
    28861 gcccgtggta tcgagtggga agcgttgctg gtggaaatgc tcggcctgtt gcatcgtatt
    28921 gcgatggtac aactttcgcc tgctgcactt ggcaacgaca tggccgccat tgagctgcgg
    28981 atgcgtgaac tggcgcgcac cataccgccg acggatattc agctttacta tcagacgctg
    29041 ttgattggtc gcaaagaatt accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg
    29101 ctgctgcgcg cgctggcgtt ccatccgcgt atgccgctgc ctgagccaga agtgccacgc
    29161 cagtcctttg cacccgtcgt gccaacggca gtaatgacgc caacccaggt gccgccgcaa
    29221 tcagcgccgc agcaggcacc gactgtaccg ctcccggaaa ccaccagcca ggtgctggcg
    29281 gcgcgccagc agttgcagtg cgtgcaggga gcaaccaaag caaaaaagag tgaatcggca
    29341 gccgctaccc gcgcgcggcc ggtgaataac gctgcgctgg aaagactggc ttcggtcacc
    29401 gatcgcgttc aggcgcgtcc ggtgccatcg gcgctggaaa aagcgtcagc caaaaaagaa
    29461 gcgtatcgct ggaaggcgac cactccggtg atgcagcaaa aagaagtggt cgccacgccg
    29521 aaggcgctga aaaaagcgct ggaacatgaa aaaacgccgg aactggcggc gaagctagcg
    29581 gcagaagcca ttgagcgcga cccgtgggcg gcacaggtga gccaactttc gctaccaaaa
    29641 ctggtcgaac aggtggcgtt aaatgcctgg aaagaggaga gcgacaacgc agtatgtctg
    29701 catttgcgct cctctcagcg gcatttgaac aaccgcggtg cacagcaaaa actggctgaa
    29761 gcgttgagca tgttaaaagg ttcaacggtt gaactgacta tcgttgaaga tgataatccc
    29821 gcggtgcgta cgccgctgga gtggcgtcag gcgatatacg aagaaaaact tgcgcaggcg
    29881 cgcgagtcca ttattgcgga taataatatt cagaccctgc gtcggttttt cgatgcggag
    29941 ctggatgaag aaagtatccg ccccatttga tcgtaagcac agcttacgtt cgtcatcctt
    30001 aacgtgattg agagagaaac ctatgtttgg taaaggcggt ctgggtaacc tgatgaagca
    30061 agcccagcag atgcaagaaa aaatgcagaa aatgcaggaa gagatcgcgc agctggaagt
    30121 caccggcgaa tctggcgcag gtctggtaaa agtgaccatc aacggtgcac acaactgccg
    30181 tcgcgtagag atcgacccga gcctgctgga agacgacaaa gagatgctgg aagacctggt
    30241 ggctgcagca ttcaacgacg cagcacgtcg tattgaagaa acgcagaaag aaaaaatggc
    30301 ctctgtatcc tccggaatgc agctgccgcc tggctttaag atgccgttct gatgcaaacc
    30361 agcccgctgt taacacagct tatggaagca ctgcgctgtc tgccgggcgt tggcccgaag
    30421 tcggcgcagc gtatggcgtt cacgctgctt cagcgcgatc gtagcggcgg gatgcgtttg
    30481 gcgcaggcgc tcacccgggc gatgtcggaa atcggccact gcgccgattg ccgcactttc
    30541 accgaacagg aagtctgtaa catctgttcg aatccgcgtc gtcaggaaaa cggtcaaatc
    30601 tgtgtggtgg agagtccggc ggacatctac gccattgagc agacggggca gttttctggt
    30661 cgttattttg tgttgatggg gcatctgtca ccgctggacg gcatcggtcc ggatgatatc
    30721 gggcttgatc gtctggaaca gcgtctggca gaggaaaaaa tcactgaagt gatcctcgcc
    30781 accaatccca cggttgaagg tgaagctacc gctaactaca ttgccgagct ttgcgcgcaa
    30841 tatgacgtgg aagccagccg aatcgctcat ggcgtaccgg ttggcggcga actggaaatg
    30901 gtcgatggca ccacgctgtc acactccctt gccgggcgtc ataagattcg tttttaagca
    30961 aacgagagca ggatcacctg ctctcgcttg aaattattct cccttgtccc catctctccc
    31021 tcatcctgtt tttaacctta aaatggcatt attgaggtag acctacatga aaggacaaga
    31081 aactcgtggt tttcagtcag aagtgaaaca gcttctgcac ctgatgatcc attctctcta
    31141 ttccaataaa gaaatcttcc tgcgtgagct tatctctaac gcctccgatg cggcggacaa
    31201 gctgcgtttc cgtgcgctat ctaacccgga cctgtacgaa ggtgatggcg aactgcgcgt
    31261 tcgtgtctct ttcgataaag acaagcgcac gctgaccatc tctgataacg gcgtggggat
    31321 gacccgcgac gaagtgattg accatctggg gactatcgct aaatccggta ccaaatcatt
    31381 cctcgaatcc ctgggttctg accaggcgaa agacagccag ctgatcggtc agtttggtgt
    31441 tggtttctac tctgcgttta tcgtggccga caaagtgacc gtgcgtactc gcgcggcagg
    31501 cgaaaaacca gaaaacggcg tcttctggga atcggctggc gaaggtgaat ataccgttgc
    31561 cgacatcacc aaagaagatc gtggtactga aatcaccctg catctgcgtg aaggcgaaga
    31621 cgagttcctc gatgactggc gcgtgcgttc catcatcagc aaatactccg accatatcgc
    31681 gctgccggta gagatcgaaa aacgcgaaga gaaagacggc gaaaccgtta tctcctggga
    31741 gaaaatcaac aaagcgcagg cgctgtggac tcgtaacaag tcggaaatca ccgatgaaga
    31801 gtacaaagag ttctacaaac acatcgccca cgactttaat gatccgctga cctggagcca
    31861 caaccgcgtt gaaggtaagc aggagtacac cagcctgctg tacattccgt cccaggctcc
    31921 gtgggatatg tggaaccgtg atcataaaca cggcctgaaa ctgtacgtcc agcgtgtgtt
    31981 catcatggac gacgcagaac agttcatgcc gaactatctg cgcttcgtgc gtggtctgat
    32041 tgactccagc gatctgccgc tgaacgtttc ccgtgaaatc ctccaggaca gcacggtaac
    32101 gcgtaacctg cgcaatgcgc tgaccaagcg tgtgctgcaa atgctggaaa aactggcgaa
    32161 agacgacgcg gaaaaatacc agaccttctg gcaacagttt ggcctggtac tgaaagaagg
    32221 tccggcggaa gatttcgcta accaggaagc gatcgccaaa ctgctgcgtt ttgcttctac
    32281 ccataccgat tcttctgcgc agaccgtatc tctggaagac tacgtttccc gcatgaaaga
    32341 agggcaggag aaaatctact acatcaccgc agacagctat gcggcagcga agagcagccc
    32401 gcacctggaa ctgctgcgta agaaaggcat cgaagttctg ctgctttccg accgcatcga
    32461 tgagtggatg atgaactatc tgactgagtt cgacggtaaa ccgttccagt cggtgtctaa
    32521 agttgacgag tcgctggaaa aactggctga cgaagttgat gagagcgcga aagaagcgga
    32581 gaaagcactg actccgttca tcgaccgtgt gaaagccctg ctcggcgagc gcgtgaaaga
    32641 tgtccgtctg actcaccgtc tgaccgatac gccagcgatc gtttcgaccg acgcggacga
    32701 aatgagcact cagatggcga aactgttcgc tgcggcgggc cagaaagtgc cagaagtgaa
    32761 atacatcttc gaactgaacc cggatcacgt actggtgaaa cgtgcggcag atactgaaga
    32821 tgaagcgaag ttcagcgagt gggtagaact gctgctggat caggcgctgc tggcagaacg
    32881 cggcacgctg gaagatccga acctgtttat tcgtcgtatg aaccagctgc tggtttcctg
    32941 atgtcatgcc ggatgacctt tgtgtcatcc ggcatttttc ttttcatcat ctgcactttc
    33001 cgcaaattat ctcgccatta accgtttcag cctcaggtgc ctttcttgag gcaatcgcct
    33061 gttggtggta tcgtttatcg ctttttcaaa aaaatcgaca cattttaagg ggattttcgc
    33121 aatgcgtatc attctgcttg gcgctccggg cgcggggaaa gggactcagg ctcagttcat
    33181 catggagaaa tatggtattc cgcaaatctc cactggcgat atgctgcgtg ctgcggtcaa
    33241 atctggctcc gagctgggta aacaagcaaa agacattatg gatgctggca aactggtcac
    33301 cgacgaactg gtgatcgcgc tggttaaaga gcgcattgct caggaagact gccgtaatgg
    33361 tttcctgttg gacggcttcc cgcgtaccat tccgcaggca gacgcgatga aagaagcggg
    33421 catcaatgtt gattacgttc tggaattcga cgtaccggac gaactgattg ttgatcgtat
    33481 cgtaggccgc cgcgttcatg cgccgtctgg tcgtgtttat cacgttaaat tcaatccgcc
    33541 gaaagtagaa ggcaaagacg acgttaccgg tgaagaactg actacccgta aagacgatca
    33601 ggaagaaacc gtgcgtaaac gtctggttga ataccatcag atgactgcac cgctgatcgg
    33661 ctactacttc aaagaagcgg aagcgggtaa caccaaatac gcgaaagttg acggcaccaa
    33721 gtcggttgct gaagttcgcg ctgatctgga aaaaatcctc ggctaatcaa gccgcagcag
    33781 atctgctttc gggcaggtct gctcatccac ctcaggcaat acctctcaca gcaattagtt
    33841 cttcttcctc acttttccgc tacaattatc aacaagttga atcgataaga ggcggtaatg
    33901 cgtcagacta aaatcggtat cctgctggca aacctgggta cgcccgatac ccccacacct
    33961 gaagcggtaa aacgctatct gaaacaattt ttaagcgaca gacgcgtggt tgatacctca
    34021 cggttgttat ggtggccatt gctgcgcggc gttattttgc cgctgcgctc gccgcgtgtg
    34081 gcgaagctgt acgcctcggt ctggatggaa ggtggctcgc cgctgatggt ttacagccgc
    34141 cagcaacagc aggcgctggc acaacgttta ccggagacgc ccgtagcgct gggaatgagc
    34201 tacggctcgc catcactgga aagcgccgtc gatgaactcc tggcagagca tgtagatcat
    34261 attgtggtgc tgccgcttta tccgcaattc tcctgttcta cggtcggtgc ggtatgggat
    34321 gaactggcac gcattctggc gcgcaaacgt agcattccgg ggatatcgtt tattcgtgat
    34381 tacgccgata accacgatta cattaatgca ctggcgaaca gcgtacgcgc ttcttttgcc
    34441 aaacatggcg aaccggatct gctgctgctc tcttatcatg gcattcccca gcgttatgca
    34501 gatgaaggcg atgattaccc gcaacgttgc cgcacaacga ctcgcgaact ggcttccgca
    34561 ctggggatgg caccggaaaa agtgatgatg acctttcagt cgcgctttgg ccgggaaccc
    34621 tggctgatgc cttataccga cgaaacgctg aaaatgctcg gagaaaaagg cgtaggtcat
    34681 atacaggtga tgtgcccggg ctttgctgcg gattgtctgg agacgctgga agagattgcc
    34741 gagcaaaacc gtgaggtctt cctcggtgcg ggcgggaaaa aatatgaata tattccagcg
    34801 cttaatgcca cgccggaaca tatcgaaatg atggctaatc ttgttgccgc gtatcgctaa
    34861 agctgagcgg taaagaactg agcgccgtcg cgaagagcct cgtcggcggt tttcatcatc
    34921 cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga gtttgaactc acagggctgc
    34981 tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat ccagcagcgg atcgaactcc
    35041 gccccggcaa taaaacaggg cggaacttcg cgagtgagat cattattaaa cagacagtaa
    35101 tacggtgact cgcggtccgc gtcgttgctt aaatacgcct cttcatacat ctgcaaatcc
    35161 tgttgcgtta agccatccca gacaccgccc aacagacgac gagtcacgga atcccgtaat
    35221 ccgtaaagcc cataccacag caaaacaccc gcaattttac cgcaatcgat ctgtttatca
    35281 cgcaaccaca acgcactggc gagcgccagc atggcacctg cggaatcacc ggcaaagcca
    35341 atgcgggaca tattgatttg ataatcctcc gcctgctggt ggaaataaca acaagcagcc
    35401 acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg aaagggtgta atcaatacca
    35461 atcaccgtac attggctgta gcttgccagc aggcgcatga tgcgatcgtg ggtatcgaga
    35521 ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg tcgccgggct atctggctgc
    35581 ggacaaaaga gacgtgtttc cacctgccca tattttgttg gaaccatgta agctctggtt
    35641 gccatttctg gagcgcccgc attccagaat cggcgctcaa gcgtgtaata ctgtcgttgc
    35701 tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg gctgaagagt attcacaacg
    35761 gtcttcattt cagcagaaat aaggtccaga acaggtagtt tgttttccgg cttcatacaa
    35821 aaactccttt caaattacgt cattgtaagg aaccactgcc atgaaaatgc gatcccgcct
    35881 gctgatattg aaactggctg cgtctcgcgc gctcccgtca gattgtgtta acattcgccg
    35941 ctcagttaac cacccgtaaa aacaaccatg aaatttcccg gtaaacgtaa atccaaacat
    36001 tacttccctg taaatgcacg cgatccgctg cttcagcaat tccagccaga aaacgaaacc
    36061 agcgccgcct gggtcgtagg tattgaccag acgctggtcg atattgaagc gaaagtggat
    36121 gatgaattca ttgagcgtta tggattaagc gccgggcatt cactggtgat tgaggatgac
    36181 gtagccgaag cgctttatca ggagctaaaa cagaaaaacc tgattaccca tcagtttgcg
    36241 ggtggcacca ttggtaacac catgcacaac tactcggtgc tcgcggacga ccgttcggtg
    36301 ctgctgggcg tcatgtgcag caatattgaa attggcagtt atgcctatcg ttacctgtgt
    36361 aacacttcca gccgtaccga tcttaactat ctacaaggcg tggatggccc gattggtcgt
    36421 tgctttacgc tgattggcga gtccggggaa cgtacctttg ctatcagtcc aggccacatg
    36481 aaccagctgc gggctgaaag cattccggaa gatgtgattg ccggagcctc ggcactggtt
    36541 ctcacctctt atctggtgcg ttgcaaaccg ggtgaaccca tgccggaagc aaccatgaaa
    36601 gccattgagt acgcgaagaa atataacgta ccggtggtgc tgacgctggg caccaagttt
    36661 gtcattgccg agaatccgca gtggtggcag caattcctca aagatcacgt ctctatcctt
    36721 gcgatgaacg aagatgaagc cgaagcgttg accggagaaa gcgatccgtt gttggcatct
    36781 gacaaggcgc tggactgggt agatctggtg ctgtgcaccg ccgggccaat cggcttgtat
    36841 atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc agcatccgct gctgccgggc
    36901 gctatagcgg aattcaacca gtatgagttt agccgcgcca tgcgccacaa ggattgccag
    36961 aatccgctgc gtgtctattc gcacattgcg ccgtacatgg gcgggccgga aaaaatcatg
    37021 aacactaatg gagcggggga tggcgcattg gcagcgttgc tgcatgacat taccgccaac
    37081 agctaccatc gtagcaacgt accaaactcc agcaaacata aattcacctg gttaacttat
    37141 tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga gctatcaggt actgaaccag
    37201 cattcacctc gtttaacgcg cggcttgccg gagcgtgaag acagcctgga agagtcttac
    37261 tgggatcgtt aagttatcgt cggttcgtag gccagataag gcgttcacgc cgcatctggc
    37321 atttggctct cgatgcctga tgcgacgctg gcgcgtctta tcatgcctac atatttttca
    37381 tattttacat ccggcaacca ccgtttaccc cgtcaccact tcacccgccg gtggcgtttc
    37441 cagcagttcc agcatagtac gggcaatttc acgttcaccc atcactacct gattcgcgcc
    37501 acgttcggtg atatacgtca cttcatcgtc ataatgggcg cgggcgataa tctcaatatc
    37561 cgggtttttc gcgcgggcag acgccacaat ctcacccgct tcataaccgt tggggatcgt
    37621 caggatcagc cattttgcac attccagatg cgccagttgc ataatttctt cgttcgctgc
    37681 attgcccaat acagcgcgga ccccacgctc tcgcagctca tcaacacggg ttcgtgacgt
    37741 ctcaatcacc accagcggaa tatcagaggc gagcaatttc tcccccagca ggctgcctac
    37801 acgaccgtaa cccaccaata gcgcatggtt gcaaatatcc actgggatct gtttctcttc
    37861 ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg gtcttcgcca gatatttctc
    37921 cagtagtgcg aacagtaccg ggttgagcat aatcgacagg atcgcccctg ccagtaccag
    37981 gttttgtccg gcctgcggca gtaaattcaa tgccattccc agtcccgcca ggataaacgc
    38041 gaactcacca atctgcgcca ggctggcggc gatggttaat gccgtacgtt gggagtgacc
    38101 aaacagacgg acaaggaaca gggccgcgac cgatttacca aacagaataa ttgccagcgt
    38161 cgccagcact gccagcggtt gctgaatcag aattaacgga tcaaacaaca tcccgacgga
    38221 gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc gtatcgtggg cggcacggtg
    38281 actcagttca gactcgttca gtaccatccc ggcaaagaac gcgccaagtg cgaaggagac
    38341 gtcaaacaac tccaccgcac caaaggcaat ccctaacgcc agcgccagca ccgacagggt
    38401 aaatagctcg cgagaaccgg ttgccgcgct gcgtgccata atccacggca ccagacggcg
    38461 acctaccagc atcataatgg cgataaatgc gatcactttg ccgatggtga tccccatatc
    38521 gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc attcctgcca ctgcgggcag
    38581 caacaccagc gtcagaacca ttaccaggtc ttccacaatc aaccaaccga tggcgatttg
    38641 cccacgctga ctgtcaatta attgccgttc ttcaagtgcg cgcagtaaca ccacggtact
    38701 ggcggtggaa agacataaac cgaacacgat accggtcatt aacgaccagc ccagcacggc
    38761 agagagcgcc atacccagca gcgtcgccac ggctatctgg gcgatcgcac cgggaatggc
    38821 gatggccttt accgccatca aatccttcag cgaaaagtgc aaaccgacgc caaacatcaa
    38881 cagaatgacg cccagttcag ccagttccgg cgcaagcttg gtatcggcaa caaagcccgg
    38941 agtgaatggt cctgccagca cacccgctaa cagatatccc accagaggag aaatacgtag
    39001 tttattggcc agcatgccga ggataaaggc gagcacaagg ccgccaacaa tggtggtgat
    39061 aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt ggttattgtc catttttggc
    39121 cgggaaaacc aaaattacag gtaatagttt atgacaattt cattgatgat gttcatgaat
    39181 aattattgaa ttttgcagaa aaatggaatt agctgcaaaa aaagcacgga tcggaaaaca
    39241 gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg
    39301 cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata tggtcaacat
    39361 ccccaatagt ggcaggaaag cacagatttt atagactaac tcgatgct
//
LOCUS       DAZPJP010000018        38832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPJP010000018 DAZPJP010000000
VERSION     DAZPJP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.018"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            25..1074
                     /locus_tag="ACHU9J_000843"
     CDS             25..1074
                     /locus_tag="ACHU9J_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1904463.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1148..2005
                     /gene="nfo"
                     /locus_tag="ACHU9J_000844"
     CDS             1148..2005
                     /gene="nfo"
                     /locus_tag="ACHU9J_000844"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1904464.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2009..3097
                     /locus_tag="ACHU9J_000845"
     CDS             2009..3097
                     /locus_tag="ACHU9J_000845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1904465.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3153..4403)
                     /locus_tag="ACHU9J_000846"
     CDS             complement(3153..4403)
                     /locus_tag="ACHU9J_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1904466.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4503..5444)
                     /gene="rihB"
                     /locus_tag="ACHU9J_000847"
     CDS             complement(4503..5444)
                     /gene="rihB"
                     /locus_tag="ACHU9J_000847"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1904467.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5574..6272
                     /locus_tag="ACHU9J_000848"
     CDS             5574..6272
                     /locus_tag="ACHU9J_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR1904468.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6339..7589)
                     /locus_tag="ACHU9J_000849"
     CDS             complement(6339..7589)
                     /locus_tag="ACHU9J_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1904469.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7683..8621)
                     /gene="psuG"
                     /locus_tag="ACHU9J_000850"
     CDS             complement(7683..8621)
                     /gene="psuG"
                     /locus_tag="ACHU9J_000850"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1904470.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8609..9550)
                     /locus_tag="ACHU9J_000851"
     CDS             complement(8609..9550)
                     /locus_tag="ACHU9J_000851"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1904471.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10008..11699)
                     /gene="fruA"
                     /locus_tag="ACHU9J_000852"
     CDS             complement(10008..11699)
                     /gene="fruA"
                     /locus_tag="ACHU9J_000852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1904472.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11716..12654)
                     /gene="fruK"
                     /locus_tag="ACHU9J_000853"
     CDS             complement(11716..12654)
                     /gene="fruK"
                     /locus_tag="ACHU9J_000853"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1904473.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VANVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12654..13784)
                     /gene="fruB"
                     /locus_tag="ACHU9J_000854"
     CDS             complement(12654..13784)
                     /gene="fruB"
                     /locus_tag="ACHU9J_000854"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1904474.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14152..15333
                     /gene="setB"
                     /locus_tag="ACHU9J_000855"
     CDS             14152..15333
                     /gene="setB"
                     /locus_tag="ACHU9J_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1904475.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15330..15584)
                     /locus_tag="ACHU9J_000856"
     CDS             complement(15330..15584)
                     /locus_tag="ACHU9J_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1904476.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15739..16311
                     /gene="yeiP"
                     /locus_tag="ACHU9J_000857"
     CDS             15739..16311
                     /gene="yeiP"
                     /locus_tag="ACHU9J_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1904477.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16534..18000
                     /locus_tag="ACHU9J_000858"
     CDS             16534..18000
                     /locus_tag="ACHU9J_000858"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1904478.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18118..19104
                     /gene="yeiR"
                     /locus_tag="ACHU9J_000859"
     CDS             18118..19104
                     /gene="yeiR"
                     /locus_tag="ACHU9J_000859"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1904479.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19143..19856
                     /gene="lpxT"
                     /locus_tag="ACHU9J_000860"
     CDS             19143..19856
                     /gene="lpxT"
                     /locus_tag="ACHU9J_000860"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1904480.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20268..20834
                     /gene="mepS"
                     /locus_tag="ACHU9J_000861"
     CDS             20268..20834
                     /gene="mepS"
                     /locus_tag="ACHU9J_000861"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1904481.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21015..22571
                     /locus_tag="ACHU9J_000862"
     CDS             21015..22571
                     /locus_tag="ACHU9J_000862"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1904482.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22653..24467
                     /locus_tag="ACHU9J_000863"
     CDS             22653..24467
                     /locus_tag="ACHU9J_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1904483.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24468..25562
                     /locus_tag="ACHU9J_000864"
     CDS             24468..25562
                     /locus_tag="ACHU9J_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1904484.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25562..26587
                     /locus_tag="ACHU9J_000865"
     CDS             25562..26587
                     /locus_tag="ACHU9J_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1904485.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26589..28178
                     /gene="yejF"
                     /locus_tag="ACHU9J_000866"
     CDS             26589..28178
                     /gene="yejF"
                     /locus_tag="ACHU9J_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1904486.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28182..28526)
                     /locus_tag="ACHU9J_000867"
     CDS             complement(28182..28526)
                     /locus_tag="ACHU9J_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1904487.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28859..29916)
                     /locus_tag="ACHU9J_000868"
                     /pseudo
     CDS             complement(28859..29916)
                     /locus_tag="ACHU9J_000868"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(30007..30702)
                     /gene="rsuA"
                     /locus_tag="ACHU9J_000869"
     CDS             complement(30007..30702)
                     /gene="rsuA"
                     /locus_tag="ACHU9J_000869"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1904488.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30852..32612
                     /locus_tag="ACHU9J_000870"
     CDS             30852..32612
                     /locus_tag="ACHU9J_000870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1904489.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32737..33021
                     /gene="rplY"
                     /locus_tag="ACHU9J_000871"
     CDS             32737..33021
                     /gene="rplY"
                     /locus_tag="ACHU9J_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1904490.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33160..34167)
                     /gene="yejK"
                     /locus_tag="ACHU9J_000872"
     CDS             complement(33160..34167)
                     /gene="yejK"
                     /locus_tag="ACHU9J_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1904491.1"
                     /translation="MGLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34349..34576
                     /locus_tag="ACHU9J_000873"
     CDS             34349..34576
                     /locus_tag="ACHU9J_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1904492.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34596..36356
                     /gene="yejM"
                     /locus_tag="ACHU9J_000874"
     CDS             34596..36356
                     /gene="yejM"
                     /locus_tag="ACHU9J_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1904493.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36431..36507
                     /locus_tag="ACHU9J_000875"
     tRNA            36431..36507
                     /locus_tag="ACHU9J_000875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36465..36467,aa:Pro,seq:ggg)
     gene            complement(36610..38832)
                     /gene="yejO"
                     /locus_tag="ACHU9J_000876"
                     /pseudo
     CDS             complement(36610..38832)
                     /gene="yejO"
                     /locus_tag="ACHU9J_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         9545 a        10127 c         9422 g         9738 t
ORIGIN      
        1 tatgcccggt taaaggagag ggttatgacg aatatcacct tacagaaaca acatcgtaca
       61 ctgtggcatt ttattccggg gttagccctg agtgcagtta tcaccggggt cgccctgtgg
      121 ggtggttcca ttcccgcggt tgcgggtgcc gggtttagtg ccctcaccct cgcaatcttg
      181 ttggggatgg ttttaggcaa caccatctat ccgcacatct ggaaaagctg tgacggtggt
      241 gtgctgtttg ccaagcaata tttattacgt ctgggtatca ttctttatgg cttccgtctg
      301 acgttctcgc aaattgccga tgtcggtatc agtgggatca tcattgacgt gttgacgctg
      361 tccagtacct tcctgctggc ttgcttcctg gggcagaaag tgtttggtct ggataagcac
      421 accagctggt tgatcggtgc cggtagcagt atctgtggtg ctgccgcggt actggcgact
      481 gagtcggtag tgaaagcgga agccagtaaa gtaaccgtgg ctgttgcaac cgttgttatc
      541 ttcgggaccg tcgcgatttt cctctacccg gcgatatatc cgctgatgtc tcaatggttt
      601 agtccggaaa ctttcggtat ctacattggt tctactgtgc acgaagtggc gcaggtggtg
      661 gcggcaggtc atgccatcag cccggatgcg gaaaacgcag cagttatttc caaaatgctg
      721 cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg cgcgtgttaa acagctgtct
      781 ggggcgaaca gcggcgagaa aagcaaaatc actattccgt ggtttgccat cttgttcatc
      841 gtcgttgcca tctttaactc gttccacctg ttaccgcaga gcgtggtgaa catgctggta
      901 acgctggata ccttcctgct ggcaatggcg atggcggcgc tgggtctgac cactcacgtc
      961 agcgcgctga aaaaagctgg ggcgaaacct ctgctgatgg cactggtact gtttgcctgg
     1021 ctgattgttg gtggtggtgc gattaactat gtgattcaaa gcgtcatcgc ataaaccact
     1081 acatcttgct cctgttaacc cgctatcatt accgttttcc tccagcgggt ttaacaggag
     1141 tcctcgcatg aaatacattg gagcgcacgt tagtgctgct ggcggtctgg caaatgccgc
     1201 aattcgcgcc gccgaaatcg acgcaaccgc gtttgccttg ttcaccaaaa accaacgtca
     1261 gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa ttcaaagccg cctgtgaaaa
     1321 atatcactac acatcggcgc aaattcttcc ccacgacagt tatctgatta acctcggaca
     1381 tccggtcact gaagctctgg aaaaatcgcg cgatgccttt atagatgaaa tgcagcgttg
     1441 cgaacaactg gggctttctt tgctcaactt ccaccctggc agccatctga tgcagatttc
     1501 agaagaggat tgccttgcgc gtattgccga atccatcaac attgcgctgg ataaaactca
     1561 aggtgtaaca gcggtgattg aaaacaccgc cggtcagggc agtaacttag ggtttaaatt
     1621 cgaacatctc gcggcgatta tcgacggcgt ggaagataaa tcccgcgtcg gcgtctgcat
     1681 tgatacctgc catgctttcg ctgccgggta tgatttgcgt actccagccg aatgcgaaaa
     1741 aacgttcgct gattttgccc gtattgtcgg ctttaagtat ctgcgcggga tgcaccttaa
     1801 cgatgcgaaa agtacctttg gcagccgcgt tgaccgccat catagcctcg gtgaaggcaa
     1861 tatcggtcat gatgcgttcc gctggatcat gcaagacgac cgtttcgacg gcattccgct
     1921 gatccttgaa accatcaacc cggatatctg ggcagaagag atcgcctggc tgaaagcgca
     1981 acaaactgaa aaagcggtgg cctgatcgat gaacgacagg gaaaagcaga ttcttaaaat
     2041 tttacggcgt aacccgctga ttcaacagaa tgaaatcgcc gatattctgc aaatcagccg
     2101 ctcacgtgtt gccgctcata ttatggattt gatgcgcaag ggtttgatta aaggcaaagg
     2161 atatatcctt accgagcagg attactgtgt ggttgtgggt gcaatcaata tggatattcg
     2221 cggaatggcg gatatccgtt acccgcaagc agcctctcat ccaggttctg ttcactgttc
     2281 cgcaggcggc gtcggacgta atatcgcgca taacctggct ctgcttggtc gggatgtgca
     2341 tttaatatca gctattggca acgattttta cggcgagaca ttactggaag aaacgcgccg
     2401 cgcgggcgtg aacgtttcaa actgcattcg tctgcatgga cacagcactg caacttatct
     2461 cgctatcgct aataagcagg aagaaactat cctggcaatc aatgacacac atattctgca
     2521 acagctaacg cctcaactgc tcaatacttc acgggacttg attcgccatg ctggtgtggt
     2581 gctggctgat tgtaatctca cacctgaagc cctggagtgg gtctttacca ttgctgatga
     2641 aattccgatg tttgtcgata ccgtttccga atttaaagcg aacaaagtaa aaaactggta
     2701 cagccggatc cacactctga aaccaacaca aaatgaactg gaaattttgt gggggcaacc
     2761 gattaaagac gacaacgatc gcatcagagc tgtaaattcg ctgcatcaac aaggtgttaa
     2821 acgcattttt gtttatctga aagatgaatc tgtattttgc agcgataagg atggagaaca
     2881 atttttgctg accgcgccag cgcatacgac agtagacagt tttggtgctg acgatggttt
     2941 tatggcgggc ctggtatata gctttcttga aggatacagt ttccgcgaca gcgcccgttt
     3001 tgcggtagcc tgcgcggcaa tttcgcgcgc cagcggcagc ttaaacaacc ctaccctgtc
     3061 tgccgataac gcgctttcat tagtgccaat ggtgtaacaa tgttgccgga ttcgacgctt
     3121 gaccgcatct tatccggctc ataaattgcg cactacgcta aaccaataaa gaatcctgca
     3181 atagtagcac tcatcaggtt agaaagtgtc gccgccgcca gcgcgcgtaa accaagttgg
     3241 gcgatttccg gcgcacggtg tggcgcaacc gcagaaaacg cccccaccac caccccgata
     3301 gaaccaaagt tagcgaaacc gcacaacgcg aaagaaataa tcgccacggt tttagcatcc
     3361 agagtgccac ccgtttgcag atatggcgag aaattgagat aagcgacaaa ttcattgatc
     3421 gccagcttct gcccaatcaa actcccggca agatttgcat cactccagtc aacccccatc
     3481 acccacgcca atggggccaa caggtaacct aaaatcgact ccagcgaagc atgttcaaaa
     3541 ccaaaccagc cgccaacacc gccgataata ccgttaatca acgcaattat tgcaacaaat
     3601 gccataacaa ctgtcgctac accggcggcg attttcagcc cggtcattgc ccctgtcgca
     3661 gcggcttcaa taatgctttt tggcggtgtt tcggtgaaag agaggttatt aaaagaaacc
     3721 tgtgaagatt ccgtagccgg gcttaacagg cgggcaaaca agatcccgcc agggatcgcc
     3781 attaacgatg ccgccagcaa atattccaca ggtacgccca gtgcggcgta accaatcatt
     3841 gtcgaaccag caatcgaggc catgccacta caaatcgctg taaataattc attgcgattc
     3901 agacgatcga taaagggttt gacgatcgcc ggaatttcgt tttgcccgag gaaaatggtg
     3961 gtgaccgcga cgaatgactc gatcttgctg atatttaaag ctttctggaa gataccgccg
     4021 agaatgcgaa ttaagatccc catcacaccg atgtagtaga gaatactcac cagtgcagtg
     4081 acgaagataa ttgccggtaa taccctgaaa ccaaagataa atcctgcgcc atcaaataag
     4141 gtatccattt tcggtccgac cagagaaccg aagataaatg cgctacctgc gtcgctgtac
     4201 gccatcactt tgtggacgcc aaaagcgact ttttctgcga cccaacgccc tggcggtaac
     4261 caaagcataa tcccgccaat cacgacctgt aacactaacg ccgcgccaac ggtacgcagg
     4321 ctgatcttct tcttgtttac tgacagtaaa aacgcaattg ccagcagtac caccattccc
     4381 agaacacttc tcatgacatc catagtgatt atctcttcat gtcattttgg gcgacggctt
     4441 tctgcccatc agaaatagcc gggcctgcat cttgcgatac tgtcccggct tgttatcaac
     4501 acttaatggg tttttatgta gccgcgtacg cactcttcga ctaatcccca gaaccagtcg
     4561 gtatcaatag tgatgccgac tttagtattg gcgggcttgc caagaacgcc cagctcgtcg
     4621 cagacggtac gcccatagca agggccactg ttgacgtcca cttcgacgta catctcctgg
     4681 gttttaatgc catcagggtt aatcagataa ccgatgcagg tggcgtcgtg caccgggccg
     4741 ccagccaggc cgtagttttc gaactgcgtt ttgagagtga agttcatgat gtcgctgaac
     4801 atctctccgg cagggccgcc tgccctttcc attcgtgcaa ttacgtccgg ggtgcaaacg
     4861 gtctggttgg taagatcgag gcccatcatc actaatggaa cgccggaggt gaacaccacg
     4921 cgtgcggctt ccgggtcggc aaagatattg aactcggcag atggggtgaa gttgcctgta
     4981 ccgtaagcgc cgcccattag cacaatttca cggattttgg gcaggattgc gggttgcata
     5041 cgcattgcca ccgcgatatt tgaaagcgga ccaaccggca ccagagtgat atcgccatcg
     5101 cttgccataa gggtatcaat gatgtatttc accgcgtggg tactttctgc ctggcgggtc
     5161 agcggctcga ataccgggcc atccagtccg gtttcaccgt gaatattatc ggcaacgatt
     5221 tgttgacgca taatgggttg cggcatcccc gcataaaccg gaacattaat ctccagtttc
     5281 tggcaaacat tcaggccatt aattaatgtt ttatcaagcg tctgattacc cgcaacaatg
     5341 gtgataccta ataaatcaat cgccggatgt ttcgccgcca tcattatagc aatagcatca
     5401 tcatgacccg gatcacaatc cagaataatt tttctctttt ccattgttta tttcctctgt
     5461 ttccagttgc gttatttttt ctacagcaac gaaatttcga tcgggccgat gattgaatct
     5521 taacaacagc gtacgaatgc taaatatgag aaatctcata gcggataaac atcgtgaaag
     5581 aaatccacaa taatgatctt aagcagcaat tgatgagtga atccgcgttt aaggattgct
     5641 ttttaacgga tgtttcagcc gatacgcggc tgtttcattt tttagcgcgt gactacattg
     5701 tgcaggaagg gcaacagccg tcctggctgt tttacctgac gcgaggccgc gccaggcttt
     5761 acgccacgct agctaatggt cgcgtgtcgc tgatcgattt ctttgccgcc ccctgtttta
     5821 ttggcgagat tgagttaatc gacaaagacc atgaaccgcg tgcggtgcag gctattgaag
     5881 agtgttggtg ccttgcgctc cctatgaaac attaccgccc gctgttatta aacgacacgc
     5941 tatttttacg aaaactctgc gtcaccttaa gtcataaaaa ttatcgtaat attgtttctt
     6001 taactcagaa tcaatcattt ccgttagtga atcgcctggc agcatttata ttactctcgc
     6061 aggaaggtga tctttatcac gaaaagcata cgcaagcggc agagtattta ggcgtttctt
     6121 atcgacatct tttatatgtt ctcgcgcaat tcattcacga cggtttatta attaaaagca
     6181 agaaagggta tctaattaaa aacagaaagc agttgtcagg actggcgctg gagatggacc
     6241 cggagaataa attctccggg atgatgcagt aaaaattatt ggcaatagca cgattgccgg
     6301 atgcaacgct taataaggcg tacatccggc aatgaaactc aagctaaacc aataaagaac
     6361 ccggcaatgg tcgcgctcat caggttggaa agcgtcgccg ccgccagcgc ccgtaaacca
     6421 agctgggcga tttccggcgc gcgttttggc gaaatagccg aaaacgctcc aacgacaaca
     6481 ccgatagaac caaagtttgc aaacccacag agggcgaacg agataatggc aatggtcttg
     6541 acatccagcg tgccgctcgt ttgcagatag ggcgagaaat tgagataagc aacaaactca
     6601 ttaatcgcca gtttctgccc aatcaaactt ccggcaagat ttgcatcact ccagtccacc
     6661 cccataatcc atgccaacgg ggccagcaca tagccgaaaa tgctttccag agagacattg
     6721 gcaaaaccaa accagccgcc aactccacca ataatgccgt tgatcagcgc aatgatcgct
     6781 acaaatgcca ttaccaccgt cgccacaccg gcggcgattt tcagcccggt cattgcaccg
     6841 ttcgccgcag cttcaataat gctttttggc ggtgtttcgc taaacgacag attttcaaac
     6901 gtaacttgcg aaggttccgt cgccggacta agaatacgtg caaacaggat accgccagga
     6961 atagccatca gcgaagcggc taacaaatag tcaatcggca cgcccattcc ggcatagcca
     7021 atcatcatcg aacccgcaat agacgccatg ccgctacaaa tagccgtaaa tagctcgttg
     7081 cgattcatgc gatcaataaa aggtttaacg atcgccggga tctcattttg cccgaggaaa
     7141 atagtggtga ctgcgacaaa agattcgatt ttgctgatgt tgagggcttt ctgaaaaata
     7201 ctgccaagaa tacgaatcag cagccccatt acgccaatgt agtacagcaa gctgataagc
     7261 gcggtaacga aaataatcgc cgggagtacg cgaaaggcga agataaagcc cgcaccgtca
     7321 aacagaacat ccattttggg accgaccagc gaaccaaaaa taaaggcact tcctgcgtca
     7381 ctgtaggaca tcactttatg gacgcctaat gccgcctgct ctactgccca ttttcccggg
     7441 ggaaaataga gcatgatgcc accaatggca atttgcagca gcagcgcagc gccaaccgtg
     7501 cgcaagctga tgctcttttt atttaccgac aacacaaagg caattgccag caataccacc
     7561 atccccgcaa cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg
     7621 cgtcagcgcc aggttttggt atgtttgatg agtacgggcg acggctttct gcccgccaga
     7681 aattaaccca cgagacgctg atattctttg gcaatttcgc tcgccagaat ggcgttatta
     7741 aagaccagtt gaatattgga tttcaggcta tcaccgccgg ttagctccgc aacacgcgcc
     7801 agcaggaacg gtgtactctc tttcccaata acaccctgtt cttcggcttc agcaacggct
     7861 tgatcaattg ctgcattgat ggtttcttct ggcatcgcaa actgttccgg gatcgggttc
     7921 gctaccacca ggccgccgtt caaaccggtt tgccacttca ctgccatcgc gcgagcaatt
     7981 tcgcgggcac tgtcaagacg aatgctgact tcaaatgggc tggttcggca gaaaaacgca
     8041 ggcagcgctt ttgtctgata accaattaac ggtacaccga aggtttctaa atattctgtt
     8101 gttaatccta aatcgagaat agattttgcc cccgcacaaa caacagtgac attggtattt
     8161 gccagttcct gtaaatcggc agaaatatcg aaggtatgtt ccgctccgcg atgcacacca
     8221 ccaatacctc cggttgcaaa aactttaatc ccagccagtg ctgcaataat catggttgat
     8281 gcaacggtag tcgcgccatt tttccctgca gcaacgacaa aaggtaaatc acgacgacta
     8341 actttcgtca cgttatgtcc ttcacggccc agcaattcga tttcttcttt acttaagccc
     8401 actttcatca caccgccaat aatggcgata gtggctggta ctgcgccctg tttacgaatt
     8461 gtttcttcta cttcaattgc ggtttgggca ttttgtggga atggcattcc gtgagaaatt
     8521 atggtcgatt ccagcgcaac aaccggtttt ttatttttta aagcatcctg tacttcagcg
     8581 gatatttgta ataattcagg ggacagagtt aattcagaca ttctgtgttc tccactaacg
     8641 atctaacatt ggcaattgat aattcagggt tgttggtata ttcacaggca agtgccattg
     8701 acgaacatcc ctgcgcaaat cgaacagaat cgataaacgg cataccgtct acccaacagg
     8761 aggccagccc cgccatcatg gcgtcgcctg ccccagtaac attgatgaca ttcgttttaa
     8821 taggggccga ccacccactt tcaccgttga tatcactgta gtaaacgcca tcccctccca
     8881 tgctcagcac caagcggttc aggccgtgtt gatgaaacca ggcggcgacc tttgcgacat
     8941 cttcacgtcc ggaaagtgca attccgctca acgtttcggc ttcaagacgg ttaggtttta
     9001 acgtatggat tttgctcaga tgatcgcgga ttttgacgca tttccatgcc gataccggat
     9061 caataaatac aggcgtttcc ccgctatttt ccagaaccca gaccagcgcc tcttcactaa
     9121 ggttgcaatc cgcgacaata acctttgcac cgcgaataaa ctcctgatgc tgggcaagaa
     9181 attctgcaga aatacagtca ctaatactca tatcatttat cgcgacaagc atttctcctg
     9241 tattatcgag cagtgataaa tagctggatg tattctcacc cggaacaatt aagcatttat
     9301 cgacataaac accggattga ttagtttgcg ccagtaatga ttgcccataa aaatcaccac
     9361 ctactgcact taaaagccag gcatttttcc ccagtaacgc cagattatga gcgatattcc
     9421 ttcccacgcc acctggggta aatttaattt ttccgggatt cgaatccgca taattcaatg
     9481 gatgatgtga atacccagcc acatcaatat tcgccgaacc aataattact atgtagtcct
     9541 tttcgcgcat agcacgcccc ctggcaataa attgccaacg aagaaattgg gtaaaatagc
     9601 cattacagtc aataagacta ttagagcacg tgttcagatt taaacacatg ttcataataa
     9661 gcatatttaa cgtaatgtaa atcgatcaga aaaaacgaaa tcggccagat atgaaggcgg
     9721 tcacaaagtt tgtctattta tgcagcaaca aaagagaaag ttgtgatgtg taacacacaa
     9781 tatggcaggc aagaaaaagg gcagagatgt ttctgccctc cagattgttg acaacgcgcg
     9841 ctttgttcat cctggatgtg gcgtaaacgc cttatccgac ctacaaaagc gcaaaaaatt
     9901 caatatattg cagggttcat gtaggtctga taagcatagc gcatcaggca attttgcgtt
     9961 tgtcatcatc ggggcagaga tgtctcatcc ccgataatga agataaatta tgcgtttttt
    10021 tctactattt gtgtttccgg gcgtttcagg aaggcatagg ccaaacccgc caccagcgta
    10081 ccggcaataa ttgctaccag gtaacccaat accggcgtaa tagcgccagg gatcagcaga
    10141 acaaacagac caccgtgtgg tgccatcagt ttcgcaccaa tcgccattga gattgcgcca
    10201 gtcagcgccc caccaacgat acagcacggt agcacacgca tcggatcacg agcagcaaac
    10261 ggaattgcac cttccgaaat aaagcacaga ccaagtacca gagcggcttt gccaccttcc
    10321 tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg ccagacccat tgccagcggt
    10381 ggcaccatac ctgccgccat aatcgccgcc atcgggccat aggtttgagt actcagcaga
    10441 cccacaccga atgcgtacgc tgctttgttt accggaccgc ccatgtcagt acacatcatg
    10501 ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc ccatggtctg cagccagtga
    10561 gtcaacccat cgagaatgcc agcaaccggt ttaccgatta ggtagatcat cgccagaccg
    10621 accaccagac tggaaattag cggaatgatc aggatcggtt tcagcgcctc catactctgt
    10681 ggcagtttca attgcgtact gattaacttc gcaatgtaac cagccaggaa gccagcaatt
    10741 ataccgccaa taaagccaga accggtgctg accgccagca taccgccaat cagacccggt
    10801 gtgaggcccg gacgatcggc aatggagaag gcaatataac ctgccagtac cggcaccatc
    10861 agcgcaaagg ctgaaccacc accaatctgc atcagcgccg ctgccaacgt acccggctct
    10921 ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac acagaccacc tgcaacaacc
    10981 atcggcagca tgtaagagac gcccgtcagc aagtgacggt aagcgcctgc actctctttc
    11041 ttaccttcag tggtcgctgt ttgagctttg cccgctggtt catacggcgt tgcttcagca
    11101 accgctttat ccagttcctg cgcagttttc ttcagcgcca gaccggtaga ggtgcgatac
    11161 atcggtttac cagcaaattt cgccagatcc acttcgatat ctgccgccac aatcaccaga
    11221 tccgctgctg cgacttcttc aggggtgatt gcattgcccg cgccaacaga accacgggtt
    11281 tcaactttca cccaccagcc acgttttttc gcttcggttt caatggcttc agccgccata
    11341 aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa ctacgcgttt cggaccgctg
    11401 gcagcaaccg gtgctgtcgc agcgaccggc gcagtgtaag gtttcgcatg gcctttggct
    11461 tcactcagga acagctcagg gtgcgcaact gcccgggaaa tatcacccag ccagacattt
    11521 ttaccgttca gcgcgctgtc attcgggatg gaatcaccga gaacaatcgc catttctgcg
    11581 tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg ccgccgcgcc cagcagggtc
    11641 ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa taatcagcag cgttttcatt
    11701 atgcctctcc tgctgtcagt taaaaggttg taagtcgacg cgcgccatca ttgcggccaa
    11761 ctgcggacga tcggtaatac ccacattgct ttgacttacc gccagggctg caacagctgt
    11821 cgccagacgc agtgtgtgtt cactggattc acgcatcagc aagccataaa tcaggccacc
    11881 aaccatagaa tcccctgcgc caacggtgct tacgacatcg actgacggtg gtttggcgat
    11941 ccattcgccg gaggcattaa cccaaagcgc gccttcggca cccagtgaaa taacaacatg
    12001 cgcaatacct tgttcacgta gcgcatgtgc agcttcaatc acatctttca tttcaggcag
    12061 tttacggcct gcccagattt ccaactcgcg gcggttaggt ttcaccagcc acggtgccgc
    12121 tttcaaacct gctactaacg cttcacggct actatcaaag ataatgcaag gacactgact
    12181 acgcaggcga gtcatccagt cggtgaacgc ttccgggctg acgcctgacg gtaagcttcc
    12241 gctgacacag accatatcga actgaccgag ccagctcaga gagtcagtca caaagcgttc
    12301 ccagtcggcg agggtgactt caaaacccga gaagttgaag tcggtcactt cgccgtcttt
    12361 ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca acctggaaac ggttggcaat
    12421 gcccagctcg ctgaacagtt gctgaaaacc atcctgattg tctttaccca ggaagccgcc
    12481 aacggtgaca tcaattccca ggtcttttaa tacgttggcc acgttgatgc ctttacccgc
    12541 cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg cgttcaattt ccgggcagaa
    12601 accaacaagg tcataagccg gattaagggt gatcgtagca acacgtctgc tcattatgcg
    12661 ccctccccaa gaccagcagc gatagcgtcg ccaatggctt tcagcgcctg ttcagcatct
    12721 gcaccctggg cggtaaagcg taggcgatga cctttcttaa cgccaagtgc cacaactttc
    12781 atcagactac gtccgtttgc cggtttgccg gtgccatcaa ggtttgtcac ggtaatatca
    12841 ctgttaaatt gtttaatggt attgaccagc atggtacctg gacgagcatg caggccgtgt
    12901 tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg tcggcgcatc atcgctggtc
    12961 agcagcgcca gcaacgttgc cgcatccgct ttcagcaagc ggtcagcttt attgtcgagc
    13021 aataaatcag cgagacgctt aagaaccgcg atgggctgat cgtcattcat cgccacactc
    13081 accagcatgg ctgccgtttc gccgtccaca tcaaaagcat ttgccgcacg gcttaccgca
    13141 atcgcgctac gcagattgcc ttcggcgcta tcgctcagcc agataccctg tccgagattc
    13201 agcggttgtt cattgatggc tttggtgacg aaagtggcgt caactgcccc cacctctttc
    13261 agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc tggcgacgat atccagcgtc
    13321 agcatttcgt tgtcgagctt cagctgctca ctctgctttt cgcccatcag taatgcgcga
    13381 agttcttctg ctgttgttgc tgacttcagt tgttcagcaa cggaatcatc gctcagtacg
    13441 tgggtcagct ggcgtagcag gcccagatgt tcatccgagc tggcagcaat accgattgcc
    13501 acgtacgcta cctgaccgtc accccaggtg acgccttccg ggaactgaaa tacctgaacg
    13561 ccggttttca gcacctgatc gcgggtgtcg gtagtgccgt gtggaatagc aataccattg
    13621 ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc cattgacgta gccttctgct
    13681 acattaccgg cctgcaccag cgccgcagcg acctggcgaa tcgcctcttc tttgtctccg
    13741 gccttttcgc ccggatggat gtcctgtacg gataactgga acatagttct cctctcttgc
    13801 tgaattgaaa cgattcagcc tctatgagaa aaaaagcgcc aacctggctt agggttaaag
    13861 acaagatcgc gctgaaacgt ttcaagaaag cataatactt ctgtttcagc acgcaaggaa
    13921 agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca tttttcctaa
    13981 ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc agccaatcag
    14041 caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt ctgtttattt
    14101 tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgacctg catgcataac
    14161 tcccccgcag tctccagcgc gaaatcgttt gacctgacct cgacggcgtt tttaatcgtt
    14221 gcctttctca ccggtattgc gggtgctctg caaaccccga cactcagtat tttccttacc
    14281 gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag cgctgtcatt
    14341 gggattctgg taagtcagtt tctcgctggg cgctctgata agcgcggcga tcgcaaatcg
    14401 ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc ctggaatcgc
    14461 aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc gaccgctaac
    14521 ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga ggcggtgatg
    14581 ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc accgctggct
    14641 tatgcgttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc ggtagcgttt
    14701 attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga gcttccgctg
    14761 gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgctact gctgtttgtc
    14821 atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc gctatttatt
    14881 atcaacgaac tgcatcttcc cgagaaactg gccgatgtga tgatggggac cgccgccggg
    14941 ctggaaatcc cgacgatgtt gattgccgga tatttcgcca aacgtctggg taagcgtttc
    15001 ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct gatggcgcat
    15061 tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg cattctgggc
    15121 ggcatcggga tgctctattt tcaggatctg atgcccggtc aggcgggttc agccaccacg
    15181 ctctatacca acacttcgcg cgtgggctgg atcatcgcag gatcagtggc gggcatcgtc
    15241 gccgaaatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat tatcgccact
    15301 ctgttttgct tactgcggat taaagatgtt taaggcgcgg tcagcatttc cagatcgata
    15361 agccaggtca tggcctgctg acgcgagtta ccgcacattt cagcgctggc ttgtaagcct
    15421 gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg ttcatcaagc
    15481 tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg gctggaaatt
    15541 gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg ttctcttaag
    15601 gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag caaaagtgcg
    15661 aataccactt gcccgaaagg cccgtcgcga gtactttgtc gcgatatttt tgacattttc
    15721 gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat ggtactgaat
    15781 tacaacggca aactgctgct ggttaaggat attgatattc agtcgcccac tgcccgcggt
    15841 gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa agtagaagag
    15901 cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta cgttgacttc
    15961 tcctatgtcg atggcaacga atatgtcttt atggataaag aagactatac cccgtatacc
    16021 ttcaccaaag atcagattga agaagagttg ctgtttatgc cagagggcgg catgccggac
    16081 atgcaggtgc tgacctggga cggtcaactg ctggcgcttg agctgccgca gaccgttgat
    16141 ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat ctgccagcgc ccgtaacaaa
    16201 ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag cccgggcgaa
    16261 aaaattcgta tccatatcga agaacgccgt tatatggggc gtgctgacta acttcagccg
    16321 catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc aatcttctat
    16381 tttgcgcttc ctgaacacct tacgccctca attttcactc gttgattgat cgccctcaca
    16441 cttcatcgca ttaacaatcc agaccaattt caattgctgt catataactt tacactgtca
    16501 ttgttaattt atcgttacta agacgtgact cctatgaaga caattgcctc cgttacgctc
    16561 ccgcatcatg tacacgctcc acgctacgat cgccagcagt tgcaatcacg tatcgttcat
    16621 tttggctttg gcgcctttca ccgcgctcat caggcgttac tgaccgatcg tgtgctgaat
    16681 gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggtga tcaactgatg
    16741 agccagctcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc ggacggcaat
    16801 caggtgataa ttgtcggtgc cgttcacgaa tgccttaatg caaaactgga ttccttagcg
    16861 gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac gattaccgaa
    16921 aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagtaatcc gcggattatt
    16981 cacgatctac aaacccctga agaacctcac tccgcaccgg gtattctcgt cgaagcactg
    17041 aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga caatattccc
    17101 gacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaatg ttcgccagaa
    17161 ctcgccgggt ggataaaaga acacgtcagt tttccgggaa ccatggtcga ccgcattgtt
    17221 ccggctgcaa ccgacgaatc actggcagaa atcagccagc atctgggggt aaatgatccc
    17281 tgcgcgatta gctgcgaacc gtttatccag tgggtggttg aagataactt catcgctggg
    17341 cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc atgggaagag
    17401 atgaaactgc gtatgcttaa tggcagccac tcttttctcg cttatctggg ttacctctca
    17461 ggattcgtcc atatcagtga ttgcatgcag gatcgcgtat ttcgccatgc tgccagaaca
    17521 ttaatgctgg atgagcaagc gccgacactg caaattaaag atgtcgattt aacacaatat
    17581 gcggataagt taattgcacg ttttgctaat ccgtcgctga aacataagac ctggcaaatc
    17641 gcgatggatg gcagccagaa attaccgcaa cgtatgctgg caggtattcg catacatctg
    17701 gggcgtgaaa cggactggtc gttgctggca ttaggcgttg ctggctggat gcgttacgtc
    17761 agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag cgataaaatt
    17821 cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtaa ccgccctgct ttccctgcgt
    17881 gaagttttcg gtgatgatct gccagataac ccgcattttg tgcaggccat cgaacaagcc
    17941 tggcaacaaa tcgtacaatt cggcgcacat caggcgctat taaacaccct caaaatttaa
    18001 cgatttctgc ggttaaagcg gatgaagctc accttcgtcc gctctcccct tctcttttct
    18061 gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg agccgttgtg
    18121 accaggacca acctcatcac cggttttctc ggcagcggga aaaccacgtc gattcttcat
    18181 ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga atttggggaa
    18241 gtcggaattg atggtgcttt gctcgccgat agcggcgcat tgctgaaaga gatccccggc
    18301 ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac cttactgcgt
    18361 cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc gaaacagatc
    18421 ctcgatctgt taaccgcacc agtctatgaa ctgtggatag atctgcgcgc caccttgtgc
    18481 attctcgatc cacgcctgct gctggacgaa aaaagcgcca gcaatgaaaa cttccgtgac
    18541 cagctggctg ccgcagacat cattgtcgcc aataaatccg accgtgcgac gcccgaaagt
    18601 gagcaagcgc tacagcgttg gtggcagcaa aatggtggcg atcgacaatt aattcacagt
    18661 gagcatggga aagttgacgg tcatcttctg gatttgccgc gtcgcaattt agccgagttg
    18721 cccgccagcg ccgcgcattc tcatcagcat gtcgtgaaaa aagggttagc agcgttaagc
    18781 ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg ggcaaggata tcaggcctgc
    18841 ggctggatat tcgacgctga tacggtattc gacaccattg gcattctgga atgggcgcga
    18901 cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct ggtacgaatc
    18961 aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc ggacagccgt
    19021 attgagctga tttccagcag cgaagctgac tggaatgctt tgcagagcgc gctgttgaag
    19081 cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata attagagaaa
    19141 atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc gcgctgtttc
    19201 tttcctggta tattcccgtt aatcatggat tctggttgcc gattgatgcg gatatttttt
    19261 atttctttaa tcagaaactg gtcgaaagta aggccttttt gtggctggtt gcattgacca
    19321 acaatcgcgc tttcgacggt tgttctctgc tggcgatggg aatgttgatg ctgagtttct
    19381 ggctgaaaga aaacgcccct ggcagacgac gtatcgtgat tatgggtctg gtcatgctat
    19441 taactgcagt ggtattaaac cagctgggtc aggcattaat tcctgtaaaa cgggccagcc
    19501 caacattgac ttttaccgat attaaccgcg tcagcgaact gctctctgtt cccacgaaag
    19561 atgcctcacg agatagcttt cccggcgatc acggcatgat gctgcttatt ttttcggcat
    19621 tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt tttgtggttt
    19681 ttgcatttcc cagagtaatg attggcgcac actggtttac tgacatcatt gtcggttcga
    19741 tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt gatcgattaa
    19801 tcaccttttt tgacaaatca ctaccgggaa aaaacaaaca tttccaaaac aaataactca
    19861 cagtaattaa catcatcagg cttattttta tagtgaggat aatcctgatg atgcgcaccg
    19921 tgctttcatc catcgcgcgc aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt
    19981 tcactcaatc gtactttaat tgaccaatcc cgcttattaa ctttctgtat cactttttct
    20041 tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg
    20101 ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt
    20161 tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgccgc ttggcatttt
    20221 tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc
    20281 aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt
    20341 ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta
    20401 gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg
    20461 acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgt tatcgtctgg
    20521 gcggcagcac taaaaaaggt atcgattgtt ctggttttgt acaacgtaca ttccgtgagc
    20581 aatttggctt agaacttccg cgttcgactt acgaacagca ggaaatgggt aaatctgttt
    20641 cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca acgggacgcc
    20701 atgtcggtat ttatatcggc aacaatcagt ttgtccatgc ttccaccagc agtggtgtta
    20761 ttatttccag catgaatgaa ccgtactgga agaagcgtta caacgaagca cgccgggttc
    20821 tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg
    20881 aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc
    20941 gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg
    21001 aaattaacgg aatcatgttc atacgcgctc ccaattctgg acgtaagctc ctgcttacct
    21061 gcattgtagc aggcgtgatg attgcgatac tggttagctg ccttcagttt ttagtggcct
    21121 ggcataagca cgaagtcaaa tacgacacac tgattatcga cgtacaaaag tatctcgata
    21181 cctattttgc cgacctgaaa tccactactg accggcttca gccgctgacc ttagatacct
    21241 gccagcaggc taaccccgaa ctgaccgctc gcgcggcgtt tagcatgaat gtccgtacgt
    21301 ttgtgctggt gaaagataaa aaaacattct gttcatctgc gactggtgag atggacattc
    21361 cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg gcgatcttac
    21421 ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc
    21481 tgaaaaatag cggtgtgttt gccgctctga atctcaacct gacgccttct ctcttttata
    21541 gttcacggca ggaagattac gatggcgtcg ccctcattat tggcaatact gcgctatcta
    21601 ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc cgtgaaacta
    21661 aaattgcggg cattcctctg accgttcggc tttatgcgga tgactggaca tggaacgatg
    21721 tgtggtacgc atttttactg ggcggcatga gtggaactgt cgttggcctg ctctgctatt
    21781 acctgatgag cgtacgtatg cgccccggca gagaaatcat gaccgccatc aagcgcgaac
    21841 aattttacgt ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta acgggcctgg
    21901 aagtactgct acgctggcgg catcctgtcg cgggagaaat tcccccggat gccttcatta
    21961 actttgccga atcgcaaaag atgattgtgc cgctgactca gcacctgttt gagttaattg
    22021 cccgcgatgc cgcagaatta gaaaaagtgc tgccagtagg cgtcaaattt ggtattaaca
    22081 ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt
    22141 ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag
    22201 agcaagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa attgctattg
    22261 atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc
    22321 tgaaaattga ccgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac
    22381 ttgacgcggt gctgacgctg gcgaaacgcc tcaatatgct gacggttgct gaaggggtcg
    22441 aaacgccgga acaggcgcga tggctaagcg aacgcggcgt taatttcatg caaggctact
    22501 ggattagtcg ccctttaccg ctggacgatt ttgtccgctg gctgaagaaa ccgtatacgc
    22561 cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg
    22621 gctaatgatg agtaaaagga aatccattgc agatgattgt gcgcatactg ctgctgttta
    22681 tcgctctgtt cacctttggt gtgcaggcgc aggctatcaa ggaaagctat gcctttgccg
    22741 tgctgggcga accccggtac gcgtttaatt tcaaccattt tgattatgtg aaccccgccg
    22801 cgccaaaagg tgggcagata acgttgtcag ccctcggcac cttcgataat ttcaaccgct
    22861 atgcactgcg cggcaacccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga
    22921 cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca cgctatgctg
    22981 acgattattc ctgggtggag gtcgctatta atccgcgcgc ccgttttcat gatggttcgc
    23041 ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa ggcgtgccgc
    23101 aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcaccgtta accgtgcgca
    23161 ttgagttagc taaacccagc aaagaagata tgctgagtct gttttcgctg ccgatatttc
    23221 cagaaaagta ctggaaggat cacaaactta gcgacccgct cgccacgcct ccgcttgcca
    23281 gtggtccgta ccgcgttacg tcctggaaaa tggggcaaaa tattgtctat tcccgcgtga
    23341 aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc
    23401 gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcctttg
    23461 atttgcgtat ggaaaacgac gctaaaaact gggccacgcg ttataccggt aaaaatttcg
    23521 ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc
    23581 tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtccgggaa gcaatcactc
    23641 tcgcctttga ctttgaatgg atgaacaagg cgttgtttta caacgcctgg agtcgcacga
    23701 acagttattt tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg
    23761 tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacacaa atctaccagc
    23821 cgccggtatc taaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc
    23881 tcaacgaaac gggctgggtg ctgaagggtc agcaacgcgt taatgccaca acgggtcagc
    23941 cgctcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc
    24001 agcacagcct gcaacggctg ggtatcaata tggacattcg caaggtggat aactcgcaaa
    24061 tcactaaccg catgcgcagt cgcgactatg acatgatgcc gcgcctatgg cgggcgatgc
    24121 cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat tccacttata
    24181 atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc
    24241 agggaaataa agaaaaatta ctgccgttgg ggcgagcact ggatcgcgta ttaacgtgga
    24301 attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat
    24361 tctcccaacc cgctgtacgc cctgtttaca gcctgggtat cgatacctgg tggtatgacg
    24421 ttaataaagc ggccaaactg ccgtcagcca gacaacaggg agagtagatg ggcgcttacc
    24481 tgattcgccg tctgttgctg gtgatcccaa cgttatgggc tattatcact atcaactttt
    24541 tcatcgtgca aattgcgcct ggcggtccgg tcgatcaggc catcgccgcc attgagtttg
    24601 gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgtgccagc catgcgcaaa
    24661 cgggtgtcgg caatatcagc gacagtaatt accgtggcgg acgcggatta gacccagaag
    24721 tgatcgctga gatcactcat cgctacggtt tcgataagcc gatccacgaa cgttacttca
    24781 aaatgctctg ggactacatc cgctttgatt ttggtgatag cctgtttcgc agcgcctcgg
    24841 tgctgacgct gattaaagac agtctgccgg tttccatcac cctcggattg tggagcacgc
    24901 tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagtc
    24961 gctttgacgt ctggagtagc gcatttatca tcatcggcta cgccattccg gcctttttgt
    25021 ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg ttccctctac
    25081 gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc
    25141 tgtggcatat cacgctaccg gtgctggcga cagtgattgg tggctttgcg gcgctgacca
    25201 tgctgaccaa aaactcattc cttgatgaag tgcgcaagca atacgtggtg accgcgcgtg
    25261 cgaaaggggt aagtgagaaa aacattctct ggaaacatgt gttccgcaac gccatgctgc
    25321 tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga
    25381 ttgaggtgat gttttcactc aatggtcttg gcttactggg ctacgaagcg accgtctcgc
    25441 gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga
    25501 atattgtcag tgatatcagc tatacgctgg ttgatccgcg tatagatttt gagggacgtt
    25561 aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgtc ataaccgtcg
    25621 cggctactgg tcgttatgga ttttcctcgt tttgtttggt ttgagtttgt gttctgaact
    25681 tatcgctaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttgtt
    25741 gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga
    25801 cccgtggctg aaacaacggc tggaaaataa cggctgggta ctgtgggcac cgattcgctt
    25861 tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca
    25921 aaactggctg ggaacggatg ccaacggcgg cgatgtgctg gcacgcattc tctatggcac
    25981 gcggatctcg gttctgtttg gcctgatgct gactctctgt tccagcgtga tgggcgtgct
    26041 ggcgggggcg ctacaaggct attacggcgg taaagtcgat ctctggggac aacgctttat
    26101 tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca
    26161 gcctaacttc tggtggctgc tggcaattac tgtcttgttt ggctggatga gtctggtcgg
    26221 cgtggtgcgg gcggagtttt tacgtactcg taatttcgac tacattcgtg cggcacaggc
    26281 gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgcctaatg ccatggtcgc
    26341 gaccctcacc tttttaccgt ttattttatg tagttcgata accaccctga cctcgctcga
    26401 tttcctcggc ttcggtctac cgctcggttc accgtcactc ggtgaactgc tgttacaagg
    26461 gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt
    26521 attgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc
    26581 ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt gtcggtgggt tttcgccatc
    26641 agcaaaccgt acgtacagta gtcaatgatg tttcactaca gattgaggct ggcgaaacgc
    26701 tggcgctggt gggtgagtca ggttcaggca aaagcgttac cgcgctgtca attttacgcc
    26761 tgctcccttc cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc
    26821 tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc gccatgattt
    26881 ttcaggaacc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag
    26941 tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc
    27001 ttgatcgcgt cggtatccgc caggcggcaa aacggctgac agattatccg catcagctct
    27061 ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat
    27121 taattgccga tgaaccgacc accgcactgg acgtctctgt ccaggcgcag attttacagc
    27181 tgttgcgcga actgcaaggc gagctgaata tgggcatgct gtttattact cataacctca
    27241 gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc
    27301 aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca
    27361 acagtgaacc gtcaggcgat ccggtaccgt tgccagaacc tgcctcaacg ttgctggatg
    27421 ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc
    27481 ataatgtggt ggtgaaaaac atcagtttta cgctacgggc gggtgaaaca ctgggtttag
    27541 tgggcgagtc tggttccggg aaaagcacga cgggactggc gctgctgcga ctgattacct
    27601 ctcagggcag catcgtcttt gacggtcagc cactgcaaga tttaaatcgc cgccagctgt
    27661 tacctactcg tcatcgtatt caggtggtat ttcaggatcc aaactcctcg ctcaacccac
    27721 gactcaacgt tttgcagatt attgaggaag gcttacgggt tcaccagccg acgctttctg
    27781 ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa
    27841 cacgccaccg ttatccggcg gagttctctg gtggtcagcg acaacgtatt gcgattgcca
    27901 gggcattaat tcttaagccc tcactgatca tacttgatga accgacatca tcactcgaca
    27961 aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg
    28021 cctatttgtt tatcagccac gatttgcacg ttgtccgcgc gttatgtcat caggttatcg
    28081 tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccgcaccgc
    28141 agcaggagta tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt
    28201 gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg
    28261 tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact
    28321 tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg accatgcatt atcaccatcc
    28381 gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc
    28441 ggttcaacct tcgaacctgc gaaaccagca gaccagcgaa agttctgcgg tagacgatgg
    28501 acgattgaga gttgtaatga agtcacttat tttccccgga agcacattta cttcacaatt
    28561 tatttacatc aattttaaca catcatcaac aatccgtctt tatacagatc gtaaagattg
    28621 atgcctcgcc tgcatggcct ctatggctct gatttaagta atatctgcgg gcgtggtcac
    28681 gtttgcgggg ctatatgtac ccgtttctgc gatctaactc aaccttttta actacaatga
    28741 tgtgactttt tacataaatt gattttacat aaaataaaca tatatcgggg gaatgataga
    28801 tttgtggttg acatgcatca acaaaggaag ccttttagct tcctcgttgt gcaatagatc
    28861 accgtttttt cggtcgactg gcgtacagac agaagagaat ggagctggtt gcgcagaatg
    28921 caattgacca aatcatcggc catgcagagt taaaggtcgc aagagaaagc aatgcgccaa
    28981 caattgcccc tatgccaaaa cggaaggttc ctgccagcga agatgccgtt cccgccatat
    29041 ggggaaactc atcaagaatg accgccatcg cattggatga caccatagac acgcagccca
    29101 caaacgccgc aacgccaacc accagcgacc agaatcccag ccccagcagc gcactgatga
    29161 ccatccacgc tgccataata aattgtatcc acaaccccga gcggaacata tttaacgcgc
    29221 caatgcggcg gacgaagcgg ctgttaaaga tggtcatcac gaacagaaaa acaatgttta
    29281 gcgcaaagta ataaccaaag ttttccggtg caacgtggtt gatttcaatg tagacaaacg
    29341 gtccggcgct caggaacgag aacattccgg caaagctgaa accgctggcc agcatataac
    29401 tcaggacacg cttatgacgg aacagcgccg caaagttacc aatagtggta cgaatgtgaa
    29461 aaggctggcg aggatccaga agatgtaatg ccagctcagc cacaccagca cccagccgcc
    29521 aactatcggt gccatcagcg gtgcaatggt cgtcaccagc atgacaaacg acatcatccg
    29581 cgagaactct tctttcgggt aaatatcacg catcaaagcg ttgataacca cgctggccgc
    29641 agccgcagcc agcccgtgga agaaacgcat cacaatcagc tgatcgatgg tttgtgccaa
    29701 cgcacacgcc accgcggcgg cggcaaacac cagcgtaccg ccaagaacca ccggcttacg
    29761 cccgaagctg tctgccatcg gcccgtagat taactgcccc aacgcaaagc ccagaatata
    29821 agtactcagg gtcatctgcg tactgcccgc cggtacgcca aactgcgctg aaattaccgg
    29881 tagcgcgggc agatacatat caatcgacag cggcatcaac atggccagca ggccaaggat
    29941 aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca acgggctcct gaaagtcatt
    30001 gaaaagttag acgacgctgg caatttcttc ttcagttaac ggacgatatt caccgggggc
    30061 taaatcagca tccagcgtaa tgccgccaat acgttcacga tgcagctcaa ccacatggtt
    30121 acccacggcg gcgaacatgc gtttcacctg atgataacgc ccttcgctga tggtcagacg
    30181 aacctgcgtt ggggtaatca cttccagcac cgccggctta gtgagatctt tttcgttatg
    30241 cagctgcacg cctttagcaa attgttctgc cgtatcgtca gctacgggtg ataccagcgt
    30301 caccagatag gtcttctcgc aatgatggcg cggagaagta atgcggtgcg accactgacc
    30361 atcatctgtc attagtacca gaccggtggt atcgatatcc agccgccctg cggcgtgcag
    30421 tttccacgct accggttcat ctagaaaata gagcaccgtt gggtgatcag ggtcgtccgt
    30481 ggagcaaaca taaccctgag gcttattgag catgaagtaa cgtggaccgt gttgctgcgc
    30541 cagcgggttg ccatcgtaag cgacatcatg ttcaggaagc agtttgaacg ctgcattacg
    30601 gacgatttcg ccatcgacgg tgacacgatt gccgcggatt tcacgcccgg caatagcacg
    30661 gctaacgccg agttgctgtg cgataaattt atcaagtcgc atgtgtgtga ttttgcctgt
    30721 aaaaaacgga ggtcgggtaa tgacccgaat atctgaactg ttgtctgccc agtatagcgg
    30781 tctaataaca tccctcaagg gaaaaagatc cgtggcatac tattagcaga ataatctacc
    30841 tacgcgagac catgattttt acacttcgcc catatcagca agaagccgtg gatgccacgc
    30901 tcaaccattt tcgtcgtcat aaaacccctg ccgttatcgt gctgcccacc ggtgcaggta
    30961 aaagcctggt gatagcggaa ctggcacggc tggctcgtgg tcgcgtactg gtgctggcac
    31021 acgttaaaga actggtggcg caaaaccatg caaagtatca ggcgctgggg ctggaagccg
    31081 atatttttgc cgccgggctg aagcgtaaag agagccacgg taaagtggta tttggcagtg
    31141 tgcagtcggt cgcccgtaat attgatgcct ttcagggtga attttcgctg ttgattgtcg
    31201 atgaatgtca ccgtattggt gacgatgaag agagccagta tcagcaaatc ctcactcacc
    31261 tgacaaaagt gaatccccac ttacgcctgc tggggctgac tgccacgcct tttcgattgg
    31321 gcaaaggttg gatctaccag ttccattatc acggcatggt acgcagcgat gagaaagccc
    31381 ttttccgtga ctgcatttat gagctgcccc tgcgttatat gattaaacac agctatctga
    31441 cgccgccaga acgactggat atgccagtag tgcaatatga tttcagccgc ttgcaggcac
    31501 agagcaacgg gctgttcagc gaagccgatc tcaaccgtga gctgaaaaaa cagcaacgta
    31561 ttaccccgca catcatcagc cagattatgg agttcgccga aaagcgtaaa ggagtgatga
    31621 ttttcgccgc gacggtcgaa cacgcaaaag agattgtggg attactgcct gccgaagatg
    31681 cagcactgat tactggcgac acccccggcg ctgagcgcga tgtgttaatt gaaaatttta
    31741 aagcccagcg ttttcgctat ctggtcaacg tcgcggtact gaccaccgga tttgacgccc
    31801 cgcacgtcga tcttatcgcc attctgcgcc ctaccgaatc agtgagtctt taccaacaaa
    31861 ttgtcgggcg cggtctgcgt ctcgctccgg gcaagactga ttgtttaatt cttgattatg
    31921 cgggtaatcc tcacgatctc tacgcgccgg aagttggtac accgaaaggc aaaagtgaca
    31981 acgttccggt acaggttttc tgccctgcct gcggttttgc caacaccttt tgggggaaaa
    32041 cgaccgccga cgggacattg attgaacact ttggtcgccg ctgtcaggga tggtttgaag
    32101 atgacgacgg tcatcgcgaa cagtgtgact tccgtttccg ttttaaaaat tgcccgcaat
    32161 gtaacgcaga aaatgatatt gccgctcgcc gctgccgcga atgtgacacg gtactggttg
    32221 atccggatga tatgttaaaa gcggcgctac gactgaaaga tgcgctggta ttacgctgta
    32281 gcggcatgtc tttgcagcat gggcacgacg agaaaggcga atggttgaaa atcacctatt
    32341 acgatgaaga cggtgcggat gtgagtgagc gtttccgtct gcaaacacct gcccagcgta
    32401 ccgccttcga gcagcttttt atccgcccgc atacgcgcac accgggcatc ccgctgcgct
    32461 ggatcaccgc cgccgatatc ctcgcccagc aagccttatt gcgacccccg gattttgtcg
    32521 tcgcccgcat gaaaggccag tactggcagg tgcgtgaaaa agtgttcgat tacgaaggtc
    32581 gttttcgtcg ggcgcacgaa ttacgcggtt aatggtagtt ttgattgatg tataaggcga
    32641 ttgagtatag aatctcaccc gcttttgcat acgcaaagca gatcacttac ctgttgctgg
    32701 gtcgcctgta gcaggattaa tttaagagag aaagaaatgt ttactatcaa cgcagaagta
    32761 cgtaaagagc agggtaaggg tgcgagccgc cgcctgcgtg ccgctaacaa gttcccggca
    32821 atcatctacg gtggcaaaga agcgccgctg gctatcgagc tggatcacga caaagtcatg
    32881 aacatgcaag ctaaagctga attctacagc gaagttctga ccatcgttgt tgacggtaaa
    32941 gaaatcaaag ttaaagctca ggacgtacag cgtcacccgt acaaaccgaa gctgcagcac
    33001 atcgacttcg ttcgcgctta attgctgaat aagttgtaaa aaaccccgct ccggcggggt
    33061 tttttgtatc tgcagattat gcctgattac ggtattgcta ttttttgcag gccagataag
    33121 gcgttcacgc cgcatctggc attcagaaca caaaaccgat taattgccgc cagacgtccg
    33181 gcgctgcaat tggtcgcgca aattcggcgg cgtgccttta atggtcaggg tatcggtcgc
    33241 cggctcccag aagatgcgtt cacctaacag catcgcatca aagttaatag tcaagccacc
    33301 gccgctaccg gcaaacttcg tcaactgacg cagtgtgctc cggtctgccg ggaagctctc
    33361 ttccagttcg tagccttttt cagccgcaaa ctccgtgaag ctcacttcgc ttacgcctgc
    33421 cagctctttc gacagcgatt ccagttcgat ctcttcgcct gcctgcagct gctcattaca
    33481 gtagctataa acctgctggc gcacgttctg tcgctctgct ttatccagtt gtgcttcggc
    33541 agtgaaatca tcaaccgctt gcaacagccc acggttttgc gctttcgtgt tcaatccttc
    33601 gctggccccg aggaaatcca taaagaaatc cgccactttg cgccctactc gccctttcag
    33661 gaaagtgaga tagcgggtgg actctggatt ggtttcccat tcggttaaat cgatacgcgc
    33721 aacaatatcc gcatgattga tatcaagata atgggttggg ttgatgtcga gattttcgtt
    33781 aacacgcata ctgctcaggt tgctcagaac tgccaccagc agatactcca ccgccagata
    33841 acggtagtgg caaaacagca cgaatccacc gtcagcaaaa ggatatttcg ctaattcgtc
    33901 acgcaggcga ccagttgccg cgcggctaaa tgccagaaag tcctcttcgc cctgacgctg
    33961 taagcgcagc gtctgcgcca gttcactctc ttcgctaaac aagccgtaag ctttattttt
    34021 ggcgctatag acccgatgca gttctgccac catctcaacg acggtttctg tcggttccag
    34081 caatgaatcg cgcaacacca gctcaaggtt ctgctcatca cgcttgataa gctggtgcag
    34141 agcaatctgg ttgatatcca gacccatgat aaactctcct ttaagaccgg gcggtattca
    34201 accaccgccg gttaagcgac gcaataaaag atggcggtca ttttacagaa ggcagcgtaa
    34261 cgagaatgat ttaacgcata aaaaagcaga aaaaaaaccg ttgctacggt aatatgttgc
    34321 cctttcatca actaactgat ttcgatttat gccacaaatt tcccgctaca gtgatgaaca
    34381 ggttgaacaa ttgctcgcag agctgctcaa cgtactggaa aaacataagg ctccgaccga
    34441 cctttccctg atggtgttag gcaacatggt cactaacctt atcaacacca gcattgcccc
    34501 ggcccaacgc caggcaattg ccaactcttt tgcccgcgcc ttacagtcct ctatcaacga
    34561 agacaaagcg cactaaggga aacagataac aggttatggt aactcatcgt cagcgctacc
    34621 gtgaaaaagt ctcccagatg gtcagttggg ggcactggtt tgcactgttc aatattctgc
    34681 tttcgctcgt cattggcagc cgttacctgt ttatcgccga ctggccgaca acgcttgctg
    34741 gtcgcattta ttcctacgta agcattatcg gccatttcag cttcctggtg ttcgccacct
    34801 acttgctgat cctcttcccg ctgaccttta tcgtcggctc ccagaggctg atgaggtttt
    34861 tgtccgtcat tctggcaacg gcgggaatga cgctattact gatcgatagc gaagtcttta
    34921 ctcgtttcca tctccatctt aatcccatcg tctggcagtt ggttatcaac ccagacgaaa
    34981 atgagatggc gcgcgactgg cagctgatgt tcatcagcgt gccggttatt ttattgcttg
    35041 aactggtgtt tgcgacgtgg agctggcaaa agctgcgcag cctgacgcgt cgtcgacgct
    35101 tcgcgcgccc gctggccgca ttcttattta tcgcctttat cgcctcgcat gtggtgtata
    35161 tctgggccga tgccaacttc tatcgcccga tcaccatgca gcgcgctaac ctgccgcttt
    35221 cgtacccgat gacggcgcga cgttttcttg agaagcatgg tctgcttgat gcgcaggagt
    35281 atcaacgccg tcttattgag caaggtaatc cagacgccgt ttccgttcag tatccgttaa
    35341 gcgaactgcg ctatcgcgat atgggcaccg ggcagaatgt gctgttgatt actgtcgatg
    35401 gcctgaacta ctcacgcttc gagaagcaga tgcctgcgct ggcaggtttt gctgagcaaa
    35461 atatttcgtt cacgcgccat atgagctccg gcaacactac agacaacggc atctttggcc
    35521 tgttctatgg tatctcgccg agctatatgg acggcattct gtcgacccgt acgcctgcgg
    35581 cattaattac tgcgcttaat cagcaaggct atcagctggg gttattctca tcagatggct
    35641 ttaccagccc gctgtatcgc caggcattgt tgtcagattt ctcgatgccg agcgtacgca
    35701 cccaatccga cgagcagacc gccacgcagt ggatcaactg gctgggccac tacgcacaag
    35761 aagataaccg ctggttctcg tgggtttctt tcaatggcac taacattgac gacagcaatc
    35821 agcaggcatt tgcacggaaa tatagccggg cggcaggcaa tgtcgatgac cagatcaacc
    35881 gcgtgctcaa tgcactgcgt gattctggca aactggacaa tacggttgtg attatcactg
    35941 ccggtcgggg tattccgctg agcgaagacg aagaaacctt tgactggtcc cacggtcatc
    36001 tgcaggtgcc attagtgatt cactggccag gcacgccggc gcagcgtatt aatgcgctga
    36061 ctgatcatac cgatctgatg acgacgctga tgcaacgcct gctacatgtc agcacacctg
    36121 ccagcgaata ttcgcaaggt caggatttgt tcaaccctca acgccgtcat tactgggtta
    36181 ctgcagcgga taacgatacg ctggcaatta ccaccccgaa aaagacgctg gtgctgaaca
    36241 ataacggtaa ataccgcact tacaacttac gtggtgaaag agtgaaagat gaaaaaccac
    36301 agttaagttt gttattgcaa gtgctgacag acgagaagcg ttttatcgct aactgattaa
    36361 ttataaatca gttagcgaaa tatcttactt gcaatcggtg tggaaaacgg tagtattagc
    36421 agccacgagt cggcacgtag cgcagcctgg tagcgcaccg tcatggggtg tcgggggtcg
    36481 gaggttcaaa tcctctcgtg ccgaccaaaa atcccaagaa aaaaccaacc tttacggttg
    36541 gtttttttat atctgcaatt aatttgataa acagaccgtg acacatcaca gcctgtttat
    36601 tttctgttat cagaacgtcc agaccactcc cgcctgagta ttccacggcg attctacccc
    36661 tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa acgatgacct
    36721 tatcccagcc tgataaacgc cacgggttcc agccacatca ttattaaatt tgccatcgtc
    36781 attaactttc acatggttag aatcggccta ttcctgacgc acggcggctt tcagccaggg
    36841 ttccaacgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc ccgcttcagc
    36901 gcgtaatatc cgggtatttc ccacatccgc gcgcatgccg tttgataacg tgtagtcctg
    36961 accatctgtg gtaaagccgg taaaggcgag ataggggcta acactccaca atccgtcaac
    37021 ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat cgccaaacgc
    37081 tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa ctttcaccac
    37141 cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagccccca gggtgtagct
    37201 atcaatattg cctttgccgc cgcgatcaaa accaatatca gaatgagagt aatcaaagaa
    37261 caagccgcga attgtgctgc tttcttcacg ggagaaacgg ctatcgatac cgagcgtcag
    37321 gcccgtcaat gtttgctcaa agccagctcc cgcatcagtg gtcacgttgt tgcgggtgtt
    37381 aattgccgaa ctccacatcg ccgtatcgta attaacgcct tttacgctac caagacgctc
    37441 acgcacggtg tccagttctg catcaaatac cagcggttgt gctgctgcca tgttcagcac
    37501 atcagtggtt gaaggggtaa tttgcgcgcg attctctgcg aggctccagc tatggttgcc
    37561 attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg cattgcccaa
    37621 cgtgaatgca gcatcaccgc cgcccgtcgt taccagtgta aggctatctc ctgctgccgg
    37681 gctggccccc gtgtccgtca cgaatatttt gaaatcacct gttgcctgac cgatgacgtt
    37741 gagctgatcg ctctgatgat tagccatatc ggtacgcata taaaattgcc gtttcccgac
    37801 agggtattgg tggtcaacgt aatgaaattt tccgcacttg ccgtcacaga tgaacgagtc
    37861 actggctcag ccatcataat cacatttgcg ccggacatcg caaggttggc taagttgagg
    37921 cgagtgtttt ggttcgtcgt agtgatatcg gcaatgatgg tccatgcgct attttcgagc
    37981 gaaacgtccg cttggctggt atccacggca ccatgcgttc taaaagaagc gccctcagaa
    38041 accactacct tacccaccaa catagcgggt gcataattta ttttcggctt catgacctca
    38101 agaatgccat cattccctct gactgacacg ttaaccattg tgccagccca gacattagcg
    38161 cggccattgt tgataaccat attttcagag atggctttag aggaatacgt gatacttcca
    38221 ttctgatctg atcgaccgag atcatataca gcatttgcct gcactttcgt accagtatcc
    38281 tttcccagcg attgcatagt ggcatgctca tcaaggatag tgtcaatggc ctgagtgtcc
    38341 tccataacaa tgagcccgga gccatcatcc agttcataat tttttgacac accatccttt
    38401 atactaaatt ggcctttact gtttgtacca ctcacttcca gcgcattcgt tgagacaatt
    38461 aatttcccgc ctgctttttt atcaacgcca gttgcagtcc cgccatccat aacctccagt
    38521 aagccgccac tatccacagt ggtattataa gcgaagtcat tttcttcaac tcgcaaactt
    38581 ccgccgtttt ccaacagcat attgttagca ataccattct tgatttcgaa ctgaccgaga
    38641 cggtttgttc cgaatacctc catgacccgc gtggttgttt taatagcacc gcctgctttc
    38701 tgatctaccg caaatgctaa tcccccgtct ttaacctcca gtaatcctcc cttttcgata
    38761 gcggcaaaga cggcaatccc atctttttca acaattaagc gcccgccttt aatcgtggga
    38821 tcattcgccg cg
//
LOCUS       DAZPJP010000019        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPJP010000019 DAZPJP010000000
VERSION     DAZPJP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.019"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHU9J_000877"
     CDS             59..1423
                     /locus_tag="ACHU9J_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1904494.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHU9J_000878"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHU9J_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1904495.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHU9J_000879"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHU9J_000879"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1904496.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHU9J_000880"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHU9J_000880"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1904497.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHU9J_000881"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHU9J_000881"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1904498.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHU9J_000882"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHU9J_000882"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1904499.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHU9J_000883"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHU9J_000883"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1904500.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHU9J_000884"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHU9J_000884"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1904501.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHU9J_000885"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHU9J_000885"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1904502.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHU9J_000886"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHU9J_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1904503.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHU9J_000887"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHU9J_000887"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1904504.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHU9J_000888"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHU9J_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1904505.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHU9J_000889"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHU9J_000889"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1904506.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHU9J_000890"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHU9J_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1904507.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHU9J_000891"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHU9J_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1904508.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHU9J_000892"
     CDS             13749..14288
                     /locus_tag="ACHU9J_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1904509.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHU9J_000893"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHU9J_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1904510.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHU9J_000894"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHU9J_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1904511.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHU9J_000895"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHU9J_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1904512.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHU9J_000896"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHU9J_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1904513.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHU9J_000897"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHU9J_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1904514.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHU9J_000898"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHU9J_000898"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1904515.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHU9J_000899"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHU9J_000899"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1904516.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHU9J_000900"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHU9J_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1904517.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHU9J_000901"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHU9J_000901"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1904518.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHU9J_000902"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHU9J_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1904519.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHU9J_000903"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHU9J_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1904520.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHU9J_000904"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHU9J_000904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1904521.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHU9J_000905"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHU9J_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1904522.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHU9J_000906"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHU9J_000906"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1904523.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHU9J_000907"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHU9J_000907"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1904524.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHU9J_000908"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHU9J_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1904525.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHU9J_000909"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHU9J_000909"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1904526.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHU9J_000910"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHU9J_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1904527.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHU9J_000911"
     CDS             complement(37465..37920)
                     /locus_tag="ACHU9J_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904528.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMCSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHU9J_000912"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHU9J_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9467 a        10313 c         9733 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcacatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZPJP010000020        36768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPJP010000020 DAZPJP010000000
VERSION     DAZPJP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.020"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..134)
                     /gene="tabA"
                     /locus_tag="ACHU9J_000913"
     CDS             complement(<1..134)
                     /gene="tabA"
                     /locus_tag="ACHU9J_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1904529.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     "
     gene            complement(279..872)
                     /locus_tag="ACHU9J_000914"
     CDS             complement(279..872)
                     /locus_tag="ACHU9J_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1904530.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            943..1656
                     /gene="bdcA"
                     /locus_tag="ACHU9J_000915"
     CDS             943..1656
                     /gene="bdcA"
                     /locus_tag="ACHU9J_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1904531.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1787..2182
                     /locus_tag="ACHU9J_000916"
     CDS             1787..2182
                     /locus_tag="ACHU9J_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1904532.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2463..2597
                     /gene="pyrL"
                     /locus_tag="ACHU9J_000917"
     CDS             2463..2597
                     /gene="pyrL"
                     /locus_tag="ACHU9J_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1904533.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2601..3536
                     /gene="pyrB"
                     /locus_tag="ACHU9J_000918"
     CDS             2601..3536
                     /gene="pyrB"
                     /locus_tag="ACHU9J_000918"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1904534.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3549..4010
                     /gene="pyrI"
                     /locus_tag="ACHU9J_000919"
     CDS             3549..4010
                     /gene="pyrI"
                     /locus_tag="ACHU9J_000919"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1904535.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4083..4469
                     /gene="ridA"
                     /locus_tag="ACHU9J_000920"
     CDS             4083..4469
                     /gene="ridA"
                     /locus_tag="ACHU9J_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1904536.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4675..7371)
                     /gene="mgtA"
                     /locus_tag="ACHU9J_000921"
     CDS             complement(4675..7371)
                     /gene="mgtA"
                     /locus_tag="ACHU9J_000921"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1904537.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7512..7565)
                     /gene="mgtL"
                     /locus_tag="ACHU9J_000922"
     CDS             complement(7512..7565)
                     /gene="mgtL"
                     /locus_tag="ACHU9J_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1904538.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7750..8697
                     /gene="treR"
                     /locus_tag="ACHU9J_000923"
     CDS             7750..8697
                     /gene="treR"
                     /locus_tag="ACHU9J_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1904539.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8816..10237
                     /gene="treB"
                     /locus_tag="ACHU9J_000924"
     CDS             8816..10237
                     /gene="treB"
                     /locus_tag="ACHU9J_000924"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1904540.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10287..11942
                     /gene="treC"
                     /locus_tag="ACHU9J_000925"
     CDS             10287..11942
                     /gene="treC"
                     /locus_tag="ACHU9J_000925"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1904541.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12336..14474
                     /gene="nrdD"
                     /locus_tag="ACHU9J_000926"
     CDS             12336..14474
                     /gene="nrdD"
                     /locus_tag="ACHU9J_000926"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1904542.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14632..15096
                     /gene="nrdG"
                     /locus_tag="ACHU9J_000927"
     CDS             14632..15096
                     /gene="nrdG"
                     /locus_tag="ACHU9J_000927"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1904543.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15141..15527)
                     /gene="cybC"
                     /locus_tag="ACHU9J_000928"
     CDS             complement(15141..15527)
                     /gene="cybC"
                     /locus_tag="ACHU9J_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1904544.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15711..17063)
                     /gene="pmbA"
                     /locus_tag="ACHU9J_000929"
     CDS             complement(15711..17063)
                     /gene="pmbA"
                     /locus_tag="ACHU9J_000929"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1904545.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17157..17708
                     /gene="yjgA"
                     /locus_tag="ACHU9J_000930"
     CDS             17157..17708
                     /gene="yjgA"
                     /locus_tag="ACHU9J_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1904546.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17791..19164)
                     /gene="mpl"
                     /locus_tag="ACHU9J_000931"
     CDS             complement(17791..19164)
                     /gene="mpl"
                     /locus_tag="ACHU9J_000931"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1904547.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19340..20338
                     /gene="fbp"
                     /locus_tag="ACHU9J_000932"
     CDS             19340..20338
                     /gene="fbp"
                     /locus_tag="ACHU9J_000932"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1904548.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20371..21366)
                     /gene="yjfF"
                     /locus_tag="ACHU9J_000933"
     CDS             complement(20371..21366)
                     /gene="yjfF"
                     /locus_tag="ACHU9J_000933"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1904549.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21353..22375)
                     /gene="ytfT"
                     /locus_tag="ACHU9J_000934"
     CDS             complement(21353..22375)
                     /gene="ytfT"
                     /locus_tag="ACHU9J_000934"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1904550.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22389..23891)
                     /gene="ytfR"
                     /locus_tag="ACHU9J_000935"
     CDS             complement(22389..23891)
                     /gene="ytfR"
                     /locus_tag="ACHU9J_000935"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1904551.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24031..24987)
                     /gene="ytfQ"
                     /locus_tag="ACHU9J_000936"
     CDS             complement(24031..24987)
                     /gene="ytfQ"
                     /locus_tag="ACHU9J_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1904552.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25297..25827
                     /gene="ppa"
                     /locus_tag="ACHU9J_000937"
     CDS             25297..25827
                     /gene="ppa"
                     /locus_tag="ACHU9J_000937"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1904553.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26206..26547)
                     /locus_tag="ACHU9J_000938"
     CDS             complement(26206..26547)
                     /locus_tag="ACHU9J_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1904554.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26550..30329)
                     /gene="tamB"
                     /locus_tag="ACHU9J_000939"
     CDS             complement(26550..30329)
                     /gene="tamB"
                     /locus_tag="ACHU9J_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1904555.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30326..32059)
                     /gene="tamA"
                     /locus_tag="ACHU9J_000940"
     CDS             complement(30326..32059)
                     /gene="tamA"
                     /locus_tag="ACHU9J_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1904556.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32265..32903
                     /gene="msrA"
                     /locus_tag="ACHU9J_000941"
     CDS             32265..32903
                     /gene="msrA"
                     /locus_tag="ACHU9J_000941"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1904557.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33226..34569
                     /locus_tag="ACHU9J_000942"
     CDS             33226..34569
                     /locus_tag="ACHU9J_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1904558.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34666..34872)
                     /locus_tag="ACHU9J_000943"
     CDS             complement(34666..34872)
                     /locus_tag="ACHU9J_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1904559.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35197..35751
                     /locus_tag="ACHU9J_000944"
     CDS             35197..35751
                     /locus_tag="ACHU9J_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1904560.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35841..36752)
                     /gene="ytfI"
                     /locus_tag="ACHU9J_000945"
     CDS             complement(35841..36752)
                     /gene="ytfI"
                     /locus_tag="ACHU9J_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1904561.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8906 a         9852 c         9137 g         8873 t
ORIGIN      
        1 aacagacgat tgccttcgat atcgtgcttg ccctttggcg tttctgccgt aacgtgtgct
       61 ttgatatgct caatcgccag gcgtaactcc tggggtagcc acggctgaag attatgaata
      121 tttccgatga tcatcttttc cactccttaa ttaaaacaat gtttcatttc catcgttata
      181 cgaaaagatg atcaattccg ctagccttaa tgcgtgaatt attgtcttat cacaacttat
      241 cggcaggcgc gttcccgcct ttcgggtcaa ggcccgcatc actccttgag aatagttttt
      301 atcgcttccc ccgccagtgc agctgttgca cagagttgtt cgatggagtg cccctcccga
      361 gccttcgcag aaagccctga catcacggta ctcataaaat cagtcacaca ttgtgcgctt
      421 tgtggatgcc gcctggcgat atagtcataa atggccgttt ctgcggcgtg ataatattga
      481 acggcaatat cacgcgcttg tggatcatga ctatgaatgc cttcaagaac catacagcca
      541 gcgcagccgc cgttttggct atatctgcgc gccgcttctt ttaatacctc agccaggcac
      601 tcccctaccg gacgatcatc acgaagaata tcggcaagcg gaatagcttc cgtaccgaca
      661 tattcattga gtacacggct aaataaccca gccttactgc caaaagccgc gtagaggctc
      721 ggggggttaa taccaagata atcagtaact tcagcaacac tgacagcatc gaaacctttt
      781 tggtgaaaaa gcacttttgc cgcagagact gcctgctcag gagcgaaacg tctgggacga
      841 cctggaacgc gagattgttt tttagtgacc attacaaaac ttgttgacag aaagttaaaa
      901 cagttttgta atgcatgtta cataataaat caaggagtcc ttatgggcgc ttttacaggt
      961 aagacagttc tcatcctcgg tggcagtcgt ggtatcggtg ccgctatcgt acgtcgtttc
     1021 gtcaccgatg gggccaatgt acgattcacc tatgcggggt cgaaagatgc cgctgaacac
     1081 ctggcacaaa agactggagc gacagcagta ttcacagata gtgctgacag agacgctgtc
     1141 attgatgtcg ttcgtaagag cggcgcattg gatatcctgg tggtaaatgc aggtattggc
     1201 gtctttggcg atgccctgga attgaatgcc gacgatattg atcgcctttt caaaatcaat
     1261 attcatgctc cttatcatgc ctctgttgaa gccgcccggc agatgcccga aggcgggcgc
     1321 atcttaatca tcggctccgt gaatggcgat cgtatgcctg ttgcaggcat ggctgcttat
     1381 gccgccagta aatctgccct gcaaggcatg gcgcgcgagc tggcccgtga tttcggaccg
     1441 cgtgggatca ccatcaacgt cgtccagcca gggccaattg ataccgacgc taatcccgcc
     1501 aacgggccaa tgcgcgatat gttgcatagt tttatggcta tcaaaagaca tgggcaaccg
     1561 gaagaggtcg ctggtatggt cgcatggtta gcagggccag aagccagttt tgttaccggc
     1621 gcgatgcata ccattgatgg cgcgtttggc gcataaccga ctacgctcaa ttaagcccag
     1681 ccatttccca tgatgtctgg gttttgttta ctcacgtcgt ccgctaaaag cggctcctgg
     1741 taaatataaa tcttctgttc atgtcgtaaa tcttgaggat aaccaaatgg tagaaagaac
     1801 cgctgttttc cctgcaggcc gacattcact gtatgctgaa catcgttatt ctgcggctat
     1861 tcgttccggc gatttgctgt ttgtttccgg gcaagtagga agtcgagagg acggaacacc
     1921 agaacccgat tttcagcaac aagtcagact ggcatttgat aatttgcatg cgaccctggc
     1981 tgctgcggga tgcacttttg acgatatcat tgatgttacg agcttccata ccgatccaga
     2041 aaaccaattt gaagacatca tgacggtgaa aaatgaaata tttagcgccc caccttatcc
     2101 aaactggacg gcggtaggtg ttacatggct ggcaggcttt gattttgaaa ttaaagtgat
     2161 agcgcgcatc cctgagcagt aagcaatagt gttagccgtt cgttttcaca ctccgcccta
     2221 taagtcggat gaatggaata aaatgcatat ctgattgcgt gaaagtgaaa aaggaaaaag
     2281 caggaaatgt ctgcaattat tgataccgaa cgaccgttcc cctgcagaat cacaacaaat
     2341 aaaaatgcat gtattttgac ttataattca aataaaccgt ttgcgctgac aaaatattgc
     2401 atcaaatgct tgcaccgctt ctgacgatga gtataatgcc ggacaatttg ccgggaggat
     2461 gtatggttca gtgtgttcga cattttgtct taccgcgtct gaaaaaagac gctggcctgc
     2521 cgtttttctt cccgttgatc acccattccc agcccctcaa tcgaggggct tttttttgcc
     2581 caggcgtcag gagataaaag atggctaatc cgctatatca gaaacatatc atttccataa
     2641 acgaccttag tcgcgatgac cttaatctgg tgctggcgac agcggcgaaa ctgaaagcaa
     2701 acccgcaacc agagctgttg aagcacaaag tcattgccag ctgtttcttc gaagcctcta
     2761 cccgtacccg cctctctttc gaaacttcca tgcaccgcct gggtgccagc gtggtgggct
     2821 tctccgacag cgccaataca tcactgggta aaaagggcga aacgctggcc gataccattt
     2881 cggttatcag cacttacgtc gatgcgatag tgatgcgtca tccgcaggaa ggtgcggcgc
     2941 gcctggccac cgagttttcc ggcaatgtac cggtactgaa tgccggtgat ggctccaacc
     3001 aacatccgac gcaaaccttg ctggacttat tcactattca ggaaacccag gggcgtctgg
     3061 acaatctcca cgtcgcaatg gttggtgacc tgaaatatgg ccgcaccgtt cactccctga
     3121 ctcaggcgtt agcgaagttc gacggcaacc gtttttactt catcgcgccg gacgcgctgg
     3181 caatgccgca atacattctg gatatgcttg atgaaaaagg gatcgcctgg agtctgcaca
     3241 gctctattga agaagtgatg gcggaagtag acatcctgta catgacccgc gtgcaaaaag
     3301 agcgtctgga cccgtccgag tacgccaacg tgaaagcgca gtttgttctt cgcgccagcg
     3361 atctccacaa cgccaaagcc aatatgaaag tgctgcatcc gctgccgcgt gttgatgaga
     3421 ttgcgacgga tgttgataaa acgccacacg cctggtactt ccagcaggca ggcaacggga
     3481 ttttcgctcg ccaggcgtta ctggcactgg ttctgaatcg cgatctggta ctgtaagggg
     3541 aaatagagat gacacacgat aataaattgc aggttgaagc tattaaacgc ggcacggtaa
     3601 ttgaccatat ccccgcccag atcggtttta agctgttgag tctgttcaag ctgaccgaaa
     3661 cggatcagcg catcaccatt ggtctgaacc tgccttctgg cgagatgggc cgcaaagatc
     3721 tgatcaaaat cgaaaatacc tttttgagtg aagatcaggt agatcaactg gcattgtatg
     3781 cgccgcaagc cacggttaac cgtatcgaca actatgaagt ggtgggtaaa tcacgcccca
     3841 gtctgccgga gcgcatcgac aatgtgctgg tctgcccgaa cagcaactgt atcagccatg
     3901 ccgaaccggt ttcttccagc tttgccgtgc gaaaacgcgc caatgacatc gcgctcaaat
     3961 gcaaatactg tgaaaaagag ttttcccata atgtggtgct ggccaattaa ttgcggttgg
     4021 taataaaagt ctggctccct ataatgagcc agacttttta ccgctgtaat aaaggagaaa
     4081 tcatgagcaa aactatcgcg acggaaaatg caccggcagc tatcggtcct tacgtacagg
     4141 gcgttgatct gggcaatatg atcatcacct ccggtcagat cccggtaaat ccgaaaacgg
     4201 gcgaagtacc ggcagacgtc gctgcacagg cacgtcagtc gctggataac gtaaaagcga
     4261 tcgtcgaagc cgctggcctg aaagtgggcg acatcgttaa aaccaccgtg tttgtgaaag
     4321 atctgaacga cttcgcaacc gttaatgcta cctacgaagc cttcttcacc gaacacaatg
     4381 ccaccttccc ggcacgttct tgcgttgaag ttgcccgtct gccgaaagac gtgaaaattg
     4441 agatcgaagc gatcgctgtt cgtcgctaat cttgatggaa atccgggcta tcatgcccgg
     4501 attaagtctg atgacaaaca caaaatcgcc tgatgcgcta cgcttatcag gcctacgtga
     4561 ttcctgcaat ttattgaatt tgttggccgg ataaggcatt tacgccgcat ccggcatgaa
     4621 caaaactcac tttgtctgca atctgaatcc aggccaccat gcccagttga ttctttattg
     4681 ccagccgtaa cgacggctat aaaatccttt caccagttgg gttaacgtca tataccctgc
     4741 cagaatcgca accagccacg ggaaatagct taacggcagc gcctgcagtt gcagataact
     4801 ggccagcggt gaaaacggca atgcgatccc gacaatcatc acgatcacgg tcatgatcat
     4861 taacggccac gatgcacagc tctgaataaa cggcacacgg cgggtgcgga tcatatgcac
     4921 aatcagcgtt tgcgacagca agcccaccac aaaccatccc gactggaaca gcgtttgcgt
     4981 ttccggcgtg ttggcatgga atacccacca catcaggcaa aacgtcagaa tatcgaagat
     5041 cgagctgatc ggtccgaaga agatcataaa gcgccccaga tccgccggat tccaacgctg
     5101 cggcttttga atttgctcgt cgtcgacgtt atcaaacggg atcgccacct gtgacacatc
     5161 atacagcagg ttctgaataa gcaagtgtaa cggcaacatc ggcaggaagg gcaagaaagc
     5221 actcgccacc agcacgctga acacattacc gaagttggag ctcgccgtca ttttgatgta
     5281 tttcagcatg ttggcgaaag tgcgacgtcc ttcaataacc ccctcttcca gcaccatcag
     5341 gcttttttcc agcaggatga tatcagccgc ttcacgggca atatctaccg cgccgtccac
     5401 agaaatgccg atatccgcag cgcgtaaggc gggcgcatca ttaataccat cgcccataaa
     5461 gccaaccaca tgcccttcgc gcttcagtaa ggtcacaatg cgttctttat gcatcggcgt
     5521 cagacgagca aacagcgtgg tgcgctgtgc gagatttgcc agttcgtcgt cagatagcgt
     5581 ttcaatatca ctaccaatga ccacctctcc cgcgtccagc cccacttcat ggcacacttt
     5641 cgctgcaact aactcactgt cgccagtaag gatttttacg gtaattccac tcgcttttaa
     5701 tgccttcagt gccggagcag ttgtctcttt tggcggatca aggaaagcaa tatatccttc
     5761 gaggatcagg tcggattcat ccgcccgctg gtaatctcct tcacgcgctg gcaggtattt
     5821 cgtcgccacc gcaaccacgc gcagcccctg acgattcagc gtatcagtaa cccgcttaat
     5881 cttacgcagc atgatgtcat cgagcggcac aatctcgcca ttgtgacgca cctgcgaaca
     5941 cacattgagg atttcctgca atgcaccttt gcaaaccagc tgatggtgct cggtattttc
     6001 tgccactacc accgacatcc ggcgacgctc gaaatcaaac ggaatctcat caattttttg
     6061 ccaacggctg gccagagagc gcgctgactc ttcatccgta ccttcgagca ccgctgtatc
     6121 gagtaggttt ttaagtccgg tctgataatg actgttcaac cacgcgctat gcagcacgcg
     6181 ttcgctggtt ttaccggaga tatcggtatg attctccagc acaattttgt cctgtgtcag
     6241 ggtgccagtt ttatcagtgc acagaatatc cattgcgcca aagttctgaa tagcatccag
     6301 atgtttgacg atcactttct gtttcgacag ctttactgcg ccgcgcgcca gcgtcgaggt
     6361 aacaatcatc ggcaacattt ccggcgttaa gcctaccgcg accgaaagcg caaacagtgc
     6421 cgcttcccac cagtcgcctt tggtgtaacc gttgataagc aacaccaccg gagccatcac
     6481 cagcataaag cgaatcagca gcatactgac gcggctgatc ccctgctgaa aggcattcgg
     6541 ctcgctttcc tgctcactaa cacgccccgc cagttgacca aaccaggtgt tggctcctgt
     6601 agcaatcacc attgcttgtg ccgtgccgct caccacggtg gtgcccataa aacacagggt
     6661 gtcacactcc agcggattgc tgtgctccgg ctggcgagtg gtagcggctt tttccacggg
     6721 cagagactca ccggttaacg acgcctgagc gacgaacaga tcccgcgcct gcaagatacg
     6781 taaatctgcc gggatcatat cccccgccgc cagttttata atatcgccgg gcaccagctg
     6841 gtcgatcggg atctccagcc agccattttc gcctttgtcg ttaattacgc gcagcaccgt
     6901 cgcggtattg ctgaccatcg ctttcagggc atctgccgct ttagtggaac gtgcttcctg
     6961 aataaagttc agcaacgtag aaatagcgac cattagcgcg ataacgcccg cggcaaataa
     7021 atcttcagtg gcgtaagaaa tagcgccgag aatggtgagt aaaatattaa atgggttgcg
     7081 atagcagacc cataaatgta cccaccacgg cgacggttgt tgtgcgggta atttattttc
     7141 accatgttgt tcgcgggcag attccacttc cgcctgattt aacccttccg gatgggtgtc
     7201 gaacgttttc cacaattctt cttcgggcat caccgccatt ttcaggcaat gcgcacttaa
     7261 ggacggcggg accgccgtat tcactgtctg ctgcgcgcca ggcaatggat cgcgatgaac
     7321 cagacgggaa ggtaaatggc gaatgagccg ggtaaaaatt tctttaaaca taaggagtcc
     7381 ctccgcactg tctgaaacag tacagaaatg tcttcaggcg tggcgaagcc ttacctgaag
     7441 gtaataaagc tgagcaggga cgttaaaacg tcacggcctt acgcatccgg tagcgccgtg
     7501 aaaagacggg cttaccggaa aagttttaat ctccgtcgag ggagaggcgt gggatcaggt
     7561 tccatataac ctccggtaag tgaaaaaagc gccgcaaata ataaggagaa tccaataata
     7621 ttcgtagcat ttatggcgat ataatacttg ctggcaacta aaccaacgat aaaccagact
     7681 ttacccattg ctgaatgcac gggtaaagtt aggctcaaat aattaaacaa cacgttacag
     7741 gacaacagga tgcaaaatcg gctgactatc aaagacatcg cacgcttaag cggcgtgggg
     7801 aaatctacag tttcccgggt gctgaataac gaaagcggcg tgagccagcg cacccgcgag
     7861 cgtgttgaag cagtgatgaa tcagcatgga ttttcccctt cccgctctgc gcgcgctatg
     7921 cgtgggcaaa gcgataaagt ggtcgccatc attgttaccc gtctggattc gttgtcagaa
     7981 aatctcgccg ttcaaaccat gctgccagcg ttctatgaac aaggttacga cccaatcatg
     8041 atggaaagcc agttttcccc gcaattagtt gccgaacatt tgggggtgct gaaacggcgt
     8101 aatatcgacg gcgtagtgct gttcggtttt actggcataa cagaagaaat gttagcccac
     8161 tggcagtcat cgctggttct gctggcgcgt gacgcaaaag gctttgcttc ggtctgttat
     8221 gacgacgaag gggcaatcaa gatcctgatg caacggctgt atgaccaggg gcatcgtaat
     8281 atcagttatc tcggcgtgcc gcatagtgac gtgacaaccg gtaagcgacg tcacgaagcc
     8341 tacctggcgt tctgcaaagc gcataaactg catcccgttg ccgctctgcc agggcttgct
     8401 atgaagcaag gctatgagaa cgtggcaaaa gtgattacgc ctgaaactac tgcgttactg
     8461 tgcgcaaccg acacgctggc acttggcgca agtaaatacc tgcaagagca acgcatcgac
     8521 accttgcaac tggcgagcgt cggtaataca ccgttaatga aattcctcca tccggagatc
     8581 gtcaccgtag atcccggtta cgccgaagct ggacgccagg cggcctgcca attgatcgca
     8641 caggtaaccg ggcgcagcga accgcaacaa atcatcatcc ccgccaccct gtcctgatcg
     8701 tttcctgaac gataaattgt gatcttcgct gcatttcggg aacgttcccg tttttaaatt
     8761 tttccgcgca atatattctg cagccaacca aaaatgtcat ctgccatggg gctttatgat
     8821 gagcaaaata aaccaaacgg atatcgatcg gttgattgaa ctggtcggcg ggcgcggcaa
     8881 tattgcgacg gtgagccact gtattactcg cctgcgcttt gtcctcaacc aaccggccaa
     8941 tgccagaccg aaagaaattg agcaactccc catggtgaaa ggctgtttca ccaatgccgg
     9001 gcaatttcag gtggtgattg gcaccaacgt gggtgattac tatcaagcac taatagcgtc
     9061 aaccggacag gcgcaggttg ataaagagca ggtaaaaaaa gccgcccggc agaatatgaa
     9121 atggcatgag cagttgatct ctcatttcgc ggagatcttc ttcccgttgc tgcccgcgtt
     9181 gattagcggc ggtttgatcc tcggttttcg caatgtgatc ggcgatttgc ccatgagcaa
     9241 cggtcagacg ctggcgcaaa tgtacccttc cctgcaaacg atctacgatt ttctgtggtt
     9301 gatcggtgaa gcgattttct tctacctgcc ggtcgggatt tgctggtcag cggtgaaaaa
     9361 aatgggcggc acgccgatcc ttggtatcgt gcttggcgtg acactggttt ccccccagct
     9421 gatgaacgct tatctgctcg ggcagcagct gccggaagtg tgggactttg gcatgttcag
     9481 catcgccaaa gtgggctatc aggcgcaggt gatcccggca ctgttagccg ggctggcgct
     9541 gggcgttatt gaaactcgcc ttaaacgcat cgtaccggat tacctctatc tggtggtggt
     9601 gcccgtctgt tcgctgatcc tcgcggtgtt cctcgcccat gcgctgattg gtccgtttgg
     9661 tcgcatgatt ggcgatggcg ttgcctttgc ggtacgtcac ctgatgaccg gcagctttgc
     9721 tccgattggt gcggcattgt ttggcttcct gtacgcgccg ctggtgatca ccggcgtaca
     9781 ccagaccacc cttgctattg atttgcagat gattcaaagc atgggtggca cgccagtgtg
     9841 gccgctgatt gcgctgtcga atatcgctca gggctccgcc gtgataggca ttatcatttc
     9901 cagccgcaag cacaatgaac gcgagatctc cgtgcctgcc gctatctccg cctggcttgg
     9961 ggtcactgag cctgcaatgt acggcatcaa cctgaaatat cgcttcccga tgctgtgcgc
    10021 gatgattggt tctggtctgg caggattgct atgcggcctg aacggcgtta tggcgaatgg
    10081 tatcggcgta gggggcctgc cgggaattct ctcgattcaa ccgagctact ggcaggtatt
    10141 tgcgctggca atggctatcg ccatcatcat cccgattgta ctcacctcgt ttatctatca
    10201 gcggaaatac cgcttgggca cgctggacat tgtttaattt tcttcggggc gcaattgcgc
    10261 cccctcgcat ttgcaggaat aacgtaatga ctaatcttcc ccactggtgg caaaacggcg
    10321 ttatctacca gatttatcca aagagttttc aggacaccac gggtagcggc accggcgatt
    10381 tacgtggcgt tatccaacgc ctggactatc tgcataaact gggcgttgat gccatctggc
    10441 taaccccctt ttatgtctct ccccaggtcg ataacggtta cgatgtagcg aactatacgg
    10501 cgattgatcc cacctacggc acgctggacg attttgacga actggtgacg caggcaaaat
    10561 cgcgcgggat tcgtatcatt ctcgatatgg tgtttaacca tacctctacc caacatgcct
    10621 ggtttcgcga ggcgctgaac aaagaaagcc cttaccgcca gttttatatc tggcgcgatg
    10681 gcgaaccaga aacgccaccg aacaactggc gttcaaaatt tggcggtagt gcgtggcgct
    10741 ggcatgcgga aagcgaacag tactatttgc atctctttgc accagaacag gcggatctca
    10801 actgggagaa tccagcggta cgcgcagagc tgaaaaaagt ctgtgaattc tgggccgatc
    10861 gtggggtcga cgggttgcgc ctggatgtgg tgaatctgat ctccaaagac ccgcgtttcc
    10921 ctgaagacct ggatggcgac gggcgtcgct tctacaccga cgggccacga gcacacgagt
    10981 ttttgcacga gatgaaccgc gatgtgttta cgccacgcgg gttaatgacc gtaggtgaaa
    11041 tgtcctccac cagccttgag cattgccagc gatacgcggc actgacaggc agtgaattgt
    11101 cgatgacctt taattttcat cacctgaagg tcgattatcc cggtggtgaa aaatggacgc
    11161 tggctaaacc tgactttgtg gcgttgaaaa cattgttccg ccactggcaa caaggaatgc
    11221 acaacgtggc atggaatgcc ttgttctggt gtaaccacga tcagccgcgc attgtttctc
    11281 gctttggtga tgaaggtgaa taccgcgtgc ctgcggcaaa aatgctggcg atggtgctgc
    11341 atggcatgca gggaacgccg tatatctacc agggcgaaga gattggcatg accaacccgc
    11401 atttcacgcg cattactgac tatcgcgacg tggagagcct caatatgttt gccgagctgc
    11461 gcaacgatgg tcgtgatgcc gacgagttat tggcaatcct cgccagtaaa tcccgtgaca
    11521 acagccgcac gcccatgcaa tggagcaacg gcgataatgc cgggtttacg gctggcgaac
    11581 cgtggattgg cctgggcgat aactatcaac aaatcaacgt agaagccgcg ctggccgatg
    11641 actcctcggt gttttacacc taccaaaagt taatcgcact gcgtaagcaa gaagccatcc
    11701 tgacatgggg caattaccag gatctgctgc caaacagccc tgtattgtgg tgctatcgcc
    11761 gtgaatggaa ggggcaaacc ttgctggtca ttgccaacct tagccgtgag atccaaccct
    11821 ggcagccagg gcaaatgcgc ggcaactggc agcttgtgat gcataactac gaagaagcct
    11881 caccacaacc ctgtgccatg aatttacggc cttttgaggc tgtctggtgg ttacagaagt
    11941 aaatcttccc taagccccgg taatgccggt cattccgggg ttttgtctgt cagcatgttg
    12001 tttttgttga tttttcaacc agcaaattca ttaaaaaatt tacataacgc tgtagcgccc
    12061 gtcatccgta cgctctgctt tttactttga gctacatcaa aaaaagttaa aacatccttg
    12121 atgcaaagca ctatatatag actttaaaat gcgtcccaac ccaatatgtt gtattaatcg
    12181 actataattg ctactacagc tccccacgaa aaaggtgcgg cgttgtggat aagcggatgg
    12241 cgattgcgga aagcaccgga aaacgaaacg aaaaaaccgg aaaacgcctt tcccaatttc
    12301 tgtggataac ctgttcttaa aaatatggag cgatcatgac accgcatgtg atgaaacgag
    12361 acggctgcaa agtgccgttt aaatcagagc gcatcaaaga agcgattctg cgtgcagcta
    12421 aagcagcgga agtcgatgat gccgattatt gcgccactgt tgccgcggtt gtcagcgagc
    12481 agatgcaggg ccgcaaccag gtggatatca acgagatcca gaccgcagtt gaaaaccagc
    12541 tgatgtcggg tccatacaaa caactggctc gtgcttacat cgagtaccgt cacgatcgcg
    12601 acattgaacg tgaaaaacgc ggtcgcctga accaggagat ccgtggtctg gtcgagcaga
    12661 ccaacgcctc gttactcaac gaaaacgcca acaaagacag caaggtgatt ccaacccagc
    12721 gcgacctgct ggccgggatc gtggctaaac actatgcacg ccagcacctg ctgccgcgtg
    12781 acgtggtgca ggcacatgag cgtggcgata ttcactatca cgatctcgat tactcgccgt
    12841 tcttcccgat gttcaactgc atgttgatcg acctgaaagg catgctgacc cagggcttta
    12901 aaatggggaa cgccgagatt gaaccgccga agtcgatctc tacggcaacc gcggtaactg
    12961 cgcagattat tgctcaggtc gccagccata tttatggcgg caccaccatt aaccgtatcg
    13021 atgaagtgct ggcgccgttt gttaccgcca gctacaacaa acatcgcaag accgcagaag
    13081 agtggaacat cccggacgcc gaaggctacg ctaactctcg taccatcaaa gagtgctacg
    13141 atgccttcca gtcgctggag tacgaagtaa acaccctgca caccgccaac ggtcagacgc
    13201 cgtttgtaac ctttggtttt ggtctgggca ccagcaggga atcgcgcctg attcaggaat
    13261 cgatcctgcg taaccgtatc gcaggcctcg gtaaaaaccg taaaactgcg gtgttcccga
    13321 aactggtgtt tgcgattcgc gatggcctga accataaaaa aggcgatccg aactacgaca
    13381 tcaaacagct ggcgctggag tgcgcaagca agcgcatgta tccggatatc ctgaactacg
    13441 atcaggtagt gaaagtcacc ggttcgttta aaaccccgat gggctgccgc agcttcctcg
    13501 gcgtgtggga aaatgaaaac ggcgagcaga tccacgatgg tcgtaacaac ctcggcgtga
    13561 tcagcctgaa cctgccgcgt attgctctgg aagcaaaagg cgatgaagcc accttctgga
    13621 agctgctgga tgaacgtctg gtgctggcac gtaaggcact gatgacccgt atcgctcgtc
    13681 tcgaaggcgt gaaagcgcgc gtcgccccga tcctctatat ggaaggtgct tgtggcgtac
    13741 gtctgaatgc tgacgatgat gtttctgaaa tcttcaaaaa cggtcgtgcg tctatttcgc
    13801 tgggttacat cggcatccac gaaaccatta acgcgctgtt cggcggcgag catgtttacg
    13861 acaacgagca gcttcgcgcg aaaggtatcg ccattgttga acgtctgcgt caggcagtgg
    13921 atcagtggaa agaagaaacg ggttatggtt tcagtctcta cagcacgccg agtgaaaacc
    13981 tgtgcgaccg cttctgccgt ctcgatactg ctgagtttgg cgtggtgccg ggcgtgaccg
    14041 ataaaggtta ctacaccaac agtttccacc ttgatgtgga gaagaaggtg aacccgtacg
    14101 acaagatcga ctttgaagcg ccttacccgc cgctggcgaa cggtggtttc atttgctacg
    14161 gcgagtatcc aaacattcag cacaacctga aagcgctgga agatgtctgg gattacagct
    14221 atcagcatgt accgtattac ggcaccaata caccgattga tgagtgctac gagtgtggct
    14281 ttaccggtga gttcgagtgc accagcaaag gcttcacttg cccgaaatgt ggtaaccatg
    14341 acgcctcccg tgtgtcggta actcgccgcg tgtgcggata tttaggtagc ccggatgcgc
    14401 gtccgtttaa cgccggtaag caggaagaag ttaagcgccg cgttaaacat ttggggaatg
    14461 ggcagatagg ttaatcttca ctctgcgtta agcaaatgcc ggatgcggcg taaacgcctt
    14521 atccggccta catttggcaa cggctgtagg cctgataaga cgcgacagcg tcgcatcagg
    14581 cattgattac cggatgcagc ctacacgcgg actgataagc caggcgaaat aatgaattat
    14641 catcagtact atcctgtcga catcgtcaac ggccccggca ctcgctgcac cctgtttgtc
    14701 tccgggtgtg ttcatgaatg ccccggttgc tataacaaaa gcacatggcg ggtaaattct
    14761 ggtcagccat ttaccaaagc aatggaagac aagatcatta aagatctgaa tgacactcgt
    14821 atcaaacgcc aggggatttc cctctccggc ggcgatccgc tgcatccgca aaacgtgccg
    14881 gatattctga aactggtaaa acgcatccgc gccgagtgtc cgggtaaaga catctgggtg
    14941 tggacaggct ataaactcga cgaactcaac gctgcgcaaa tgcaggttgt tgatttgatt
    15001 aacgtgctgg tcgacggcaa atttgtgcag gatttaaaag acccttccct gatctggcgc
    15061 ggcagcagca atcaggtggt gcatcatttg cgatgataag ttccctgtta cctgagtgca
    15121 ggcaacaggg aaatgaggaa ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc
    15181 agttgctctg cagcagcctg cgcttctttt actttacctt catttgccag cttcagcgcg
    15241 tcgtcaatct gaccgaccag aatgtcgaaa ccgtggcgga aatctttcat ttccgggctg
    15301 tccggtgatt tatcttcgag cttcggcggc gttgcttttt gcgcatccag cgctgcggcg
    15361 cgcatcttcg ttaacgcgtc tttgacttgc gtcgcgttat cggctttttc gaccactttt
    15421 aaattgtcgt tgagggtttc catattgtct tcaagatcag cggcaaacga cgccgaactg
    15481 aataccaacg aggagactgc aagaatagct aacaggcttt tacgcattgc tcacttcctt
    15541 ttttatttac acttcatcct tcacactgcc tcatcgacag ctacgctcat tcgaccgggt
    15601 cacgtactac gagtacgctc cccggctctc attcactttc cgtctcgatg cagtgcgaat
    15661 gattttgtgt aattttttag tttatagcgc ggcaggtcgc gccagttttt ttactgtccg
    15721 gcgattttca tctccggcaa cagcacagaa ccacactgta tattactgcg tgtttcaata
    15781 tcgttaccga cggtgacaat attgcgccac atatctttta aattacctgc gatggtgatt
    15841 tcgctcaccg gatactgaat ttcgccattc tctacccaga agcccgcagc accacgggaa
    15901 taatcaccgg taatggcact cacgccctgg cccatcagct ccgtcaccac cagcccggtg
    15961 cccatctctt tgagcatttg ttcgaagctt agcccttgtc cggcaatccg ccagttgtga
    16021 ataccgcccg catgtccggt gcttttcagc ccaagttttc gcgccgaata gctggtcagc
    16081 agccactgag tcaggatgcc atctttaata atatcgcgac gctcggtgcg cacaccttcg
    16141 ctgtcgaatg gcgtcgacgc cagccctttc agcagatgcg gatgctcttc aatggtcagc
    16201 cagtccggca gaatttgttt acccagcgaa tccagcagga aggtagattt acgataaacc
    16261 gatccacccg ctatcgcccc caccagatgc ccaaaaagcc cagttgccac ttcattggca
    16321 aaaatgactg gcgctttcat ggtggagagt ttacgcggta acaggcgcga taaagtacgg
    16381 cgagcacagt cggccccaac ccactctggc gtttgcagat cgctcatcgc gcgcccaatg
    16441 gtgtaggcgt aatcacgctc catatcgcca ttttcttcgg caattacaca gctggagagc
    16501 gaatgacgcg ttgagcagta accctgcaac atgccgtggc tgttgccaaa aactttgaca
    16561 ccgtagtggc tgttaaagct gccaccttcg gtattggtga tacgtttatc cgcctgtaat
    16621 gctgcctgtt ctgcgcgggc cgccagttca atggcttcat ccggggaaac ttccgcaggg
    16681 tggaacaagt cgagatctgg tgcgtcaaag gccagcagct ctttgtctgc caggccggca
    16741 cagggatctg gcgaggtata acgggcaata tccagcgccg cctgtacagt acgggcaatg
    16801 gcctgcgggc ttaaatcggt ggatgatgcg ctacctttgc ggttctggtg ataaacagtg
    16861 atccccagcg cgccatcgct attgaattca acattctcca cttcaccata acgcgtgctt
    16921 acgctaatgc cggtggtctt gctgacggca acttccgcac cgtccgattt gcctgaggcc
    16981 aactccagcg cagtggaaac tgcttcttcc agaatcttgc gctgcgcttc aacttgagag
    17041 attactttca ttgcaagtgc cataatgtag agagtttctc tgaagtctaa cagagaaccg
    17101 tttttcagtg cgcatcttaa ctggtaacat tagcctcttt ttttaaggag cctgagatga
    17161 ctaagcagcc cgaagactgg ctcgacgacg ttcccggtga tgacatcgaa gacgaagacg
    17221 atgaaattat ctgggtcagt aaaagtgaaa ttaaacgtga tgccgaggag ctaaaacgcc
    17281 ttggcgcgga aattgttgat ctggggaaaa acgcgctgga taagatcccg ttagatgcgg
    17341 atctgcgcgc ggctattgag ctcgcccagc gtattaagat ggaaggtcgc cgccgccagt
    17401 tgcagctcat cggtaagatg ctgcgccagc gcgacgtaga gcctattcgt caggcgctgg
    17461 ataagctgaa aaaccgtcac aaccagcagg tggtgctgtt ccataaactg gaaaacctgc
    17521 gcgatcgtct gatcgatcaa ggtgatgacg ccatcgctga agtattgaat ctgtggcccg
    17581 atgccgatcg tcagcaactg cgtacactga tccgcaacgc gaagaaagag aaagaaggga
    17641 ataagccgcc gaaatccgca cgccagattt tccagtatct gcgcgaactg gcggagaatg
    17701 aagggtaatt ttcgttgttg atgtgagtat ggtttgccgg atgcggcgta aacgccttat
    17761 ccggcaatgc tatatcaggc tgaagccgaa ttactgcgca gcttccgcct tcttcgccag
    17821 accatccagc agtttctgat ggatcccacc aaaaccgccg ttgctcatca ccagaatatg
    17881 gtcgccaggc tgagcggttt tcaccaccat atctgccagc gtatccacat cgccactcca
    17941 gtgtgcaggc tgaacgcagg cttctgccac ttctgccacc tgccacggaa tatgcgccgg
    18001 ttgcagcagg aagacttcat cggcacgacc taatgaaggt gccagatcgt ctttgcagat
    18061 ccccattttc atggtattcg agcgcggttc cagcacagca ataatgcgcg ccgtaccacc
    18121 aactttgcca cgcagcgccg ccagcgttgc cagaatcgcc gtcgggtgat gggcaaaatc
    18181 gtcatatacc gtgacgccat tcgcttcacc acgcaactcc agacggcgac gagcattaat
    18241 aaacgaaccc agcgcgttag cggcatctgc cgacgctaca ccaacatggc gagccgctgc
    18301 aatcgccatc aggccattgt gcatattatg ttcgcctacc agcgaccatt tcacttcgcc
    18361 cactttttcg ccatccagca aaacttccca ttcggaagca tcggtggtca gctttttcgc
    18421 ctgccagtga ccctgctcac ccaccagctc ctgctcgctc cagcagccca tcgccatggt
    18481 ctgtttcagg ttgatgtcat tttccggcca gataatacgg ccctgccccg gaacgatacg
    18541 caccagatgg tggaactgtt tctggatcgc tttcaggtcg tcaaagatat cggcgtgatc
    18601 gaactcaagg ttgttgagga tcagcgtacg cgggcagtaa tggacaaatt tagagcgttt
    18661 gtcgaagaag gcgcagtcgt actcatccgc ttcgataaca aagaagtcgc tttcgcccag
    18721 acgcgccgaa acctcaaagt tccccggcac accgccgatc acaaatcccg gtttgtaacc
    18781 acactgttcc agaatccagg tcgccattcc cgcggtggtg gttttgccgt gtgtaccggc
    18841 aacggccagc acccagcggt cgcgcagcac aaaatcgtgc agccactgtg gacctgacat
    18901 ataagggatg tttttttcca gtaccgcttc cacacacgga tttccacggg tcatggcgtt
    18961 gccaataatc accagatccg gctgcggctc gagctggctg gcatcgtaac cctgaatcag
    19021 ctcaatgcct tgcttctcaa gtaaggtgct catcggcgga tacacattgg cgtccgaacc
    19081 cgttacttca tggcctaact ggcgcgccag catcgccaga ccgcccataa atgtgccaca
    19141 aattcctaaa atatgaatgc gcatacgtca ctatcctttt ttaatctgcc gctcatttta
    19201 cgcatatgtc gggcaagtga gaaacgcatt tcaggataat ccgcaatttg ctggcgcgat
    19261 tcacctgagc gcaacattga gattatttgt taagattgtt gcggtcgctt tactccataa
    19321 acattgcagg gaaagtttta tgaaaacgtt aggtgaattt attgtcgaaa agcagcacga
    19381 gttttctcat gctaccggtg agctcactgc tttgctgtcg gcaataaaac tgggcgccaa
    19441 gattatccat cgcgatatca acaaagcagg actggttgat atcctgggtg ccagcggtgc
    19501 tgagaacgcg cagggcgagg ttcagcaaaa actcgacttg ttcgctaatg aaaaactgaa
    19561 agccgcactg aaagcacgcg atatcgttgc ggggattgcc tctgaagaag aagatgagat
    19621 tgtcgtcttt gaaggctgtg aatacgcaaa atacgtggtg ctgatggacc ccctggatgg
    19681 ctcgtccaac atcgatgtta acgtctctgt cggtaccatt ttctccatct accgccgcgt
    19741 tacgcctgtt ggcacgccgg taacggaaga agatttcctc cagcctggta acaaacaggt
    19801 tgcggcaggt tacgtggtat acggctcctc taccatgctg gtttacacca ccggatgcgg
    19861 tgttcacgcc tttacttacg atccttcgct gggcgttttc tgcctgtgcc aggaacggat
    19921 gcgcttcccg gagaaaggca aaacctactc catcaacgaa ggaaactaca ttaagtttcc
    19981 gaacggggtg aagaagtaca ttaaattctg ccaggaagaa gataaatcca ccaatcgccc
    20041 gtatacctcg cgttatatcg gttcactggt tgcggatttc caccgtaacc tgctgaaagg
    20101 cggtatttat ctctacccaa gcaccgccag ccacccggac ggcaaactgc gtttgcttta
    20161 tgagtgcaac ccgatggcat tcctggcgga acaagcgggc ggtaaagcga gcgatggcaa
    20221 agagcgtatt ctggatatca tcccggaaac cctgcaccag cgccgttcgt tctttgtcgg
    20281 caacgaccat atggttgaag atgtcgaacg ctttatccgt gagttcccgg acgcgtaatc
    20341 tcctgcgcct ctccacacgg agaggcgttt ttattgctgc gcaatgttga ctcttgtcac
    20401 tggcgaactc tgacgattct cccacagcac cgttaatcca cgctgtaatg ctataaaaat
    20461 aaacaacaaa ataccgatgg cgattttcgt ccaccaggaa ctcagcgtgc catcaaagtt
    20521 tatgtaagtc tgaatcagtc cctgaatcgc cacgccaaaa agcgtcccta ataccgttcc
    20581 aacgccaccg ctcaaaagcg taccgccaat taccactgag gcgatagcat ccagctccac
    20641 acctacgccc gccagcgcat atccggcctg ggtataaatc gagaagacaa tccccgccag
    20701 cgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tggaaatccc
    20761 catcagattc gccgacgttg cgttgccgcc aatggcgtat acctgattac caaaacgggt
    20821 acgatgcgcg aggaatatgc cgataaccac caccgccaac atcagcagtc ccatcgcact
    20881 taagcgaccg ccgccaggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
    20941 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
    21001 cgcaagcgta atgatgaacg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc
    21061 gccgaaggca cagcccatca ccagcaccag cggaaacgcc agcagcggcg agaggccgaa
    21121 atcgccaatc acttttgcca gaaacacgcc ggtaaaggcg atcaccgaac cgacggagag
    21181 atcgatccca ccggagagga tcacaaaggt catgccaacg gcaatgatcc caagaaaggc
    21241 gttatcggtc aggatattgc agatcactct tgtggaagca aaaccaggaa actgcgtcag
    21301 gcagtagaga tagcccagca caaagacgcc gatggtgatc ataagcggca agttacgttt
    21361 tatcacggct acgcactcct ttaatcagac tgataaagcg ttgcgactga acaatcagca
    21421 cacaaagcac caccaccgct ttcaccacct ggttcatctc tggcggaaag cccgaaagca
    21481 aaattccggt attcattccc tgaataatca gcgccccaac tcccgaaagc agcaggttaa
    21541 agcgaccgcc catcagcgat ccaccgccaa tcaccaccgc gagaatggca tccagctcca
    21601 gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg
    21661 caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt cgtgtgttga
    21721 ctccggcatt ttttgccgcc cgaatgttga taccaacggc ttcgataaac atccccagcg
    21781 ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata atgaccggcg
    21841 ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag ttaaacgtga
    21901 cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg
    21961 tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcta
    22021 ggatgccggt gcccagagcg cttaacaaaa caatcggcag gctgaatccc gcgaccgtca
    22081 tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga tcgataccac
    22141 cggtggcgat caccagcgtc ataccaatcg ccagtaacgc aacgggagct gcacggttaa
    22201 gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc cagaaatgcg
    22261 gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc ggcatccccg
    22321 tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga ggcatcacac
    22381 tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg tgccagcggg
    22441 atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata gcccaccagc
    22501 tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag cgtttcaatc
    22561 aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttggctc atcaaggatc
    22621 agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg attgccaccg
    22681 gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag
    22741 cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc
    22801 tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc atcggttttc
    22861 ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga tcgcagggtt
    22921 tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact
    22981 tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat ctcgccgggg
    23041 cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca
    23101 acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcacgtgggt atccagctcg
    23161 cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct
    23221 acgaaactgc cgttgcgtaa gacagtgatc cgatcgctga cctgatagac ctgatcgaga
    23281 aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat
    23341 aatagctcca cttcctgggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg
    23401 gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgcgac tgaaaagcgg
    23461 ttaagcggtt cgcgcacgtc gaggaagaaa ccgtaagatg ccatcagttc ggtggcgcgc
    23521 ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc tatgaataga
    23581 ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt gccaataccg
    23641 agttgttgtg catgggcggt atttttcggt gagatagcct ggccttccag ccagatggtg
    23701 ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg
    23761 ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac
    23821 gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc ctggtgttgg
    23881 tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg
    23941 taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg
    24001 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
    24061 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
    24121 catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg ccatgttcgg
    24181 cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga tgtccggtac
    24241 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
    24301 ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc
    24361 gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc
    24421 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
    24481 tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
    24541 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
    24601 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
    24661 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc
    24721 aaccactgga gcgataaaga tcgcatccac cccttgtgca acgaaggaac gtaccgcttt
    24781 aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc
    24841 ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg
    24901 cgaaaatcct ccggttaatg gagctgccaa cgccatagac gacatggctg ccgagactgc
    24961 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
    25021 gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca
    25081 cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
    25141 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
    25201 aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatactcg
    25261 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
    25321 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
    25381 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
    25441 gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac
    25501 ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc
    25561 cgtccggttg gcgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg
    25621 gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct
    25681 gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
    25741 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct
    25801 tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc
    25861 ttcggttagt aagggttttt tatgcccgcg ataaataaac tctctattcc accatcatta
    25921 ttctcagcgg ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac cattttgctg
    25981 ttcatagcca cttgctggaa gttagccgac ctcactcata ctcaccgttc aaagcgtgcc
    26041 cccgttttac ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat
    26101 ggaaagcggc tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagat
    26161 acatcgcata aaacaacgcc acccgaaggt ggcgtgcttt tattattact tatccctgtc
    26221 taaccagtcg ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat aaacgtacat
    26281 ccatgcactc ccgtacggcg tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg
    26341 caaggcatca agttcggcca gcgtggcgtt gtcaatacga taaacttcac cgtgtaccgt
    26401 tccgttcccc ggaactgcgc ctggatagtg gcccaggcta tacaactggt agttatcgat
    26461 actgaaatcg cccagtaact gggcattggt catccagtga ctgttgcctt gtttgtggcg
    26521 taaactgccg tagacaaata ttcgcattgc taaaactcga actgatagag caaatccagt
    26581 gcctggtcta caccagacac ggcttccaga tatagcttag gcatcaggcg ataacgtaac
    26641 gtgagtgttg ctatagagtc aaatataccc acgccgtatt tcacttgcag acctggcaat
    26701 acatagccgc tgaccactac ctgggaggag tcgcctactc cctgggtgtc gagcgctaaa
    26761 ttgcttacgc caaacgtctc gccgatttta cccacaatct ggccactttg cgcaaccccc
    26821 agaccaatca gcatcgaggt cattgccgca ctgtcgctct gatcgctctc cagcccttgt
    26881 ccacgtagca aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc
    26941 gctttcggtt cgtccgccag accagtgacg cgaactccgg caattacgtc gtcttctgta
    27001 gcatccgggt tacgaatagc ttcaatatta agatacggtt gatccggcgg accagagaac
    27061 agtaactcac ccttacgcac aatcagatcc tgaccatagg catggaagcg cccttcaggg
    27121 atgttgatct gcccgttcag gcccagccct tgtttgtcct gtacgacgtt aagatcgccc
    27181 gtcagccgcg ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac gtggacaatc
    27241 aggttactgt taatcggaat cgacgccgtt ttcggctctt ccggttgcag gttatcgtta
    27301 agcatcacca catcgctgga gacgcctact gcgctttccg gcagatcgtg caccacgatg
    27361 cgcgcccacg ggacatccac gcgaccatcg agggtaaaca ggtttggtgt agcctcgaat
    27421 acaacatctg gcgatacatc cattcgtacc atcggcggca cggtgatccg cactttactg
    27481 cctttcgccg ttactcgtgc ccgccagttt tcaatttggc tccagtcggc atcaccgttc
    27541 aggtagattt caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg cataccgtta
    27601 aagttgaccg caagctggct cggctgcata tcaaacggca taaagttgcc gtcgatatcc
    27661 acacccgtaa cctgaagctg accaaacaac tgcgggcttt gcacatcacc acccagacgc
    27721 aagttggcac tcaccatccc cgctgctttt tccccacggg taaagatggg gtttatcatc
    27781 gcaaggttga agttacggat attgacgttg ccaccaagat tacggcggcc ttgcggatcg
    27841 gtcacctgca cctgtccatc aaactggccg ttattggtca ggcggatggt ccagcccaat
    27901 tcggcacggt tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc caccggcagc
    27961 gccgcatcgt tgacggtttg cgttacctgc acgttacgcc ccgaaagggt gatactgccc
    28021 tgcggcagcc cctctttcgt ggtgtcccag gcaacatccg ctttacccgt gaagataccg
    28081 ctggcctgag tggtttctgg cataaacggt ttcagcatgg cgaggtcgaa gcggttgaga
    28141 ttcaccaccg cacgcccttc ggcccccgca tcgatagttt gcggcacgca cagttccgca
    28201 ttcgggttaa gccagcagtg tggcccgatg ctgatttttt gctccttatt gcggtaatcc
    28261 agcgcaatat cgcgggtcag cgaccacggg ccgaccggcg tctggaaacg ggtattgcta
    28321 agagttccct tccagcgttc ttctttgcga tcaaaacttc ctgccagatt aagctgcccg
    28381 gagacaggct cgccctgaat ccgcaactgt agctcgtgct gcttttcgct gcctttggca
    28441 ttcagggtga cgaggttgat atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg
    28501 agtttcccgg cgatctgatc ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg
    28561 gaaagttcct gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc
    28621 accgtgccgc gtactttcac cagccctttc gctgtaccgc caagccccgg cagcgcgtta
    28681 tccagccccg gcgcgttgat ggtggcatca agattgagat cttttacccc cagctcgcct
    28741 ttcacttcgg cactgtttgg cccgagttcc agatgaagcc cagggatcat ccactgcata
    28801 taactgttgc ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac gttaccggtc
    28861 agcttcagtt ctggcacctc catctgccag gtgccgccgt acaggctacc gcgggtttta
    28921 atcaagccat tgagtttcga cggccactcc gggaactctt tggcggtgtt aatgccatta
    28981 agcgttagct caccgcgcca actaatggcc tgctgccagt cgagcaacgc cttgagttca
    29041 gttttccctt ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg ttcattacct
    29101 ttggcatcaa gggtaatggt ggctggcggg atctcctgtc ccttcactgc cgtacgcata
    29161 gagagcgtgt aatcggtcat tttgccggta agtttcagtt tcagatcatc cgcctgatac
    29221 tgcttctcac cagtgaacgg ccagtaaagc tgtttgctgt tcacttccac gttgagtggc
    29281 aatccggctt ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg accggaaagg
    29341 ttaacgccta tctccagctg ttcgcgcagc gcgccgccca ctttcagctt caccttttca
    29401 cctttcaacg gctccacgtt cagtgtgctg ttgaggatga tatccaccgg ccagttgtct
    29461 gacagctgcg ccgtgccgct ggcgttgacg atcccctggt tggaatcgat atccagggcg
    29521 tccagtttag tattgccgtc aatgctgctc actttcagca gcatggtgcg cacggtgatg
    29581 tccgtgtcgc ccgtcacgcg cagctgctcg cccttaaact cttcaatgtt caggttaagc
    29641 ggcaaatgca cgtcggtcat ttccggcaat accgggcgag aaaagagatc tttcagcgtt
    29701 tcgccgagcg gcttttcatc cggctgcgga ttttcaatct tcggttcgac gacctcttcc
    29761 tgcgccactt ccgccacttt cggcagagca atcagcaggc ctttcagcga cgtcggtttc
    29821 agggtcaggg ttttctcctg ccagttcagg ccggaggtga agtccatcac cgacaccgtg
    29881 gtgtcatcaa tcttgatgtt gacgttatcc agcgccaccc gtgtcagggt gatgggatac
    29941 ggcgtggaga gatccagcgg accgctatct tcctcttctt caacctgttc agaaggaggc
    30001 atttttttac tgtcgatgtt gacctgaatg tctttcagcg ccaggtcatt aatacaaacg
    30061 ctactgttcc acaggcactc aagaccgacc gccagatgca gattacccgc ttttaccgcc
    30121 acgcctggct gctcataacg aacgtcagac aaggtgagat cgcgccagcc gccggtgacg
    30181 ttgccaatat ccagtcctgg cacccagcga tccgccgctt taaatacaag atgcaggccg
    30241 ctggtggtgc ccaccagaaa cgccaccgat cccagcaaca gtaagataac gataaccacg
    30301 ccgaggctga tttttttcca taaactcata attctggccc cagaccgatg taaaactgta
    30361 acccgtgttc gtctttatcc gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg
    30421 gcgattccca gcgtacgccg cccccggtac cggttttaaa gtcgctgcgg cgaatatcgc
    30481 ttaccgcttc gccactatcg acaaacaccg cgccccacca ttttccggtc acgttgtact
    30541 ggtattccag cgatccggtt atcagcttcg aggccccttt caggtcaccg ttggcgtatt
    30601 tcggggcgat agatttatat ttgtagccgc gaatactacg atcgcctccg gcgaagaaac
    30661 gcagatccgg cggtactttg tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg
    30721 tcacaaaacg atggcgatcg tacagtgtgc ggatccagac gttctgcgcc tggaaaacgg
    30781 agaaatcgac atctgaaccc caggccgtgt tggagtagtc gatagagtag cgttgcgagt
    30841 cgccccaggt tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc accccaggat
    30901 aaaacagcat cgtggtgttg gtaatttcac cctgggtaaa gtggtcgagg ctccagcgca
    30961 ggttaatggc acgctgccag tcgctggaga gatcccagta gcgagaagcc accagcgtag
    31021 tggagtcaga ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc accaaataat
    31081 attgttccag tggattcttc agcagcggca ttttatagct gaagtcgagg atctgttccg
    31141 gcgcggaaat actggtactg gtggtcagac tgtgaccata agagttcatc cacggctttt
    31201 tccacgtcgc tttcacgcgc ggtcccacgt ccgtagagta accgaccccg gtttcgatgg
    31261 tgttttctgt tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt
    31321 tatcaaattg cggtgccact accaccgaat taaaccagcc ggtagcagaa agtcggcggt
    31381 tcagttccgc cagatctttc gattcatact catcgccctc tttaaacggc accagatttt
    31441 gcaggtattc atcgcggatt tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt
    31501 cgccactgtt ataatcaata tcccagaagg ctttatgcag gccgagcgca atgcccagct
    31561 gcgctttggt aaattcgcta tcgaaataac ctttacgcaa cgcaatactg gttaaggact
    31621 ttttgaaatt ttcataatcg ccctggttca gtaccgtacc aatagccggg cgagtatcga
    31681 gcaatttcaa atagtcttta tcggtccgcg cgccgccgcg caataccaca tcggtgccgc
    31741 caattaacac cggtacacct ggcgtgactt tggcgatcaa tacctgccgc cctttctttg
    31801 gcggtggacg gagatcaaat tcaatggtcg gctggtaata acccagcgct ttcagacctt
    31861 cacggatggc atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt
    31921 caatcgtaga aagctgcgca cgaacgttct tttccagctg tcccgataac ccctcgacct
    31981 gtagacggac gttcgcggcg acggcagatc cgcttaagca gagtaagctt acacagcata
    32041 actgtcggat atagcgcaca ttttctcctg aatatccttt tttcctaccc ctggaacgcc
    32101 gttaaacggc ttaacaaaaa tccagtaata tggattaaaa agcagactaa accccaaata
    32161 tttcttatgt tttactttag acctattcac ggtggttatt gtgtgcaaat acgcctcttg
    32221 ttacaacctt aaccccaatg accgattttc gggagagcga caccatgagt ttatttgata
    32281 aaaagcatct ggtttccccc gccgatgccc tgcctggacg taacaccccg atgcccgtag
    32341 ccacgctgca tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga atggagattg
    32401 ccatttttgc gatgggttgt ttctggggtg tggagcgtct gttctggcag ttacccggcg
    32461 tttacagcac cgccgcaggc tataccggcg gctatacgcc aaatccgact tatcgggaag
    32521 tgtgctccgg tgatacgggt catgccgaag cggtacgcat tgtttacgat ccttccgtca
    32581 tcagctatga gcagttgcta caggtatttt gggagaatca cgatcccgcc cagggaatgc
    32641 gtcagggcaa tgaccacggc acgcagtatc gttcagcgat ttatccgctg accccagaac
    32701 aggatgccgc agctcgcgcc agtctggaac gttttcaggc ggcgatgctt gccgccgatg
    32761 acgatcgtca catcaccacg gaaatcgcta acgccacacc gttttattat gccgaagatg
    32821 accaccagca atatctgcat aaaaacccat atggttactg tggaattggc ggaattggcg
    32881 tctgtctgcc gccggaagca tagcgttacg ggtacaaatg tagtttgttg ataaagtgct
    32941 ctttgtctat gcctgatgcg gcttaaacga cttaccaggc ctacaaaatc gtccaaattc
    33001 aatatattgc aaggactgcg taggcctgat cggcatagcg catcaggcag tattgcattt
    33061 atcagcagtc tgaattttaa ccctctggcg actttacagt accttacgct atactagcca
    33121 ctgaaaatgt cggttcactt tcttcgaatc gactttcaat gtgtatttca cacaaattaa
    33181 tcaactttcc cttccgagga tctggcctga aaggtcggat aagatatgtt aaacagtatt
    33241 ttagtcatac tctgcttgat cgctgtaagt gcgttcttct cgatgtccga gatctcgctt
    33301 gccgcctcac gcaaaatcaa acttaaactg ctggctgatg aaggcaatat aaatgcccaa
    33361 cgcgttctga atatgcagga aaatcccggc atgttcttta ccgtggtcca aatcggtctg
    33421 aacgcagtgg cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc agcttttcac
    33481 agcctgttct cccgctatat gtcggcagag ctctctgagc aactgagctt tattctctct
    33541 ttctcgttag tgactggcat gtttatcctg tttgcggatt taaccccgaa acgcatcggt
    33601 atgattgcgc cagaagcggt ggctttgcgt atcatcaacc cgatgcgctt ctgcctgtac
    33661 gtttgcaccc cactggtgtg gttcttcaac ggcctggcga acatgatctt ccgtattttc
    33721 aaactgccaa tggtacgtaa agatgacatc acttctgatg acatctacgc ggtagtggaa
    33781 gccggtgcgc tggcgggcgt gttacgtaaa caggaacacg agctgattga aaacgtcttt
    33841 gagctggaat cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt gatttggttt
    33901 gatctccacg aagatgagca aagcctgaag aataaggtgg cggaacatcc gcactctaag
    33961 ttcctcgtct gtaatgaaga tattgaccac atcatcggtt atgtcgattc taaagacctg
    34021 ctgaaccgcg tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac
    34081 acgctgattg tgccggatac gttaaccctt tcagaggcgt tggaaagttt taaaaccgca
    34141 ggtgaagact tcgcggtgat catgaacgag tacgcgctgg tggtggggat catcaccctc
    34201 aacgacgtga tgaccacgct gatgggcgat ctggtcggtc aggggctgga agagcagatt
    34261 gtcgcccgtg atgagaactc atggctgatt gacggcggca ctccgattga cgacgtcatg
    34321 cgcgtgctgg atattgacga attcccgcag tcgggcaact acgaaaccat cggcggcttt
    34381 atgatgttta tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac
    34441 aaatttgaag tggtggatat cgataactac cgcatcgacc agctgctggt gacccggatc
    34501 gacagcaagg ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc
    34561 gtcgcgtaac cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    34621 tagcactttt tactgcatag cactttttac tgcatagcac tttggttaag ccatctctgt
    34681 ttgcagacgc ataacctgac ggttaacttc ggacatcact gacaaatgca gcttatcctt
    34741 cacttttggg ataagaatct tacccttatc aaattcaaaa gcgccaacgt ccttgatgta
    34801 taaccgtcca cggaacagga tctttacgta cttcgccacc tgaagtgggt tgtaccgttg
    34861 gaaaattttc attgttatct cctgctgagt attacgcctg tgcggtgcca caatcggccc
    34921 aactattatg aggcgcaaat tttaatgctt agtgactata gactatccgg gcaatgtttc
    34981 caccgcgtat aacttttttt acctaaaggt tacaattatt cagaattatc tttttaccga
    35041 agcgcggtct tcagtataag cattcatttt tcatatgatt tgtgcgcttg accgcaaact
    35101 ggcatcacac ttgcgggaaa ttcgataaat agcacatatg attaaaactc agacccaagt
    35161 ggtcggatca cctgcatatc ataagaagga aacaccatga ccctacgcaa gattctggca
    35221 ctcacctgcc tgctgttgcc gatgatggct tccgcacatc agttcgaaac cggtcagcga
    35281 gtgccgccga ttggcattac cgatcggggc gagttggtgc ttgataaaga tcagtttagc
    35341 tacaaaacct ggaacagcgc gcagttagtg ggaaaagtgc gagtactgca acatattgct
    35401 ggtcgcacct ctgcaaaaga gaaaaacgcg acgctgattg aagcgattaa atcagcgaag
    35461 ttaccgcacg atcgttacca gaccaccact attgttaaca ccgacgacgc gattccgggt
    35521 tcagggatgt ttgtgcgcag cagtctggag agtaataaaa agctttatcc ctggtcgcag
    35581 tttattgtcg atagcaatgg cgtcgcgcgc ggtgcctggc agctggatga agaaagttcc
    35641 gctgtagtgg tgctggataa ggatggtcgc gtgcaatggg ccaaagacgg ggcgctcact
    35701 caggaagagg tgcagcaagt gatggacctg ctgcaaaagt tgctcaaata atggcaacaa
    35761 catagattct gcaaaaataa ttttccccta tcgttatcta tgggataggg gaattaagtc
    35821 tctgagttct tacgccgaac tcatactggt aacgctagtt gctctttttt aaacgcctca
    35881 atggtcgact tatatttctc gcaaatcttt tctttggtaa tattactatc tgcggccaga
    35941 ttcgcactta ctatcgcatc cacttgttcg gctaattcca cttgttggca acctgcatta
    36001 accagaaaat tataaataag ggagtaatct tcatcctcca aaacaacaga ggaaccatta
    36061 cgaaaacgtt catcccctcc atagatttta agcgcaaagc ccaaagattc tatgactatg
    36121 gcaaaattat catcaattat tttttcgtga atataccatc catcgcattc tttaattcca
    36181 cctcctggta tttggagttc aaatttaccc cgttttaatt tgcttactat atcctccaat
    36241 aaggcataac ctaaagacac gtcaccaatc gcctggtgaa tataaccttt aatccttttc
    36301 ggtatgtcag actgtttttt ctgcagaata tcacaggcat acaatcttct gttttgttct
    36361 aacggaaact cgcggttttt gaaatccatg aatgagttat actcatttat tgttatggga
    36421 atttcttcgt attcaaatcc actacctcgc aatgtcattg tatattcggc accgttatct
    36481 ttaaggctga aactgataag gaacttatcc atggcgttaa cagttccatt ttttgatgaa
    36541 agtacctgat aactattttt atcaatctga gttgcgcaac gaaagaactc cctcaaagtt
    36601 aacaggctat cataattcga aatccaatct tttttcttaa atgcatgctg gatattgtta
    36661 aaaaaattgt ttgaatgtgt ttttgtatac gatgacttta caaataactc caccgcacca
    36721 ataaaattat tgtgtctgat cctgggtaac atatgacaac cttatttt
//
LOCUS       DAZPJP010000021        36257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPJP010000021 DAZPJP010000000
VERSION     DAZPJP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.021"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            89..316
                     /locus_tag="ACHU9J_000946"
     CDS             89..316
                     /locus_tag="ACHU9J_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904562.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(393..635)
                     /locus_tag="ACHU9J_000947"
     CDS             complement(393..635)
                     /locus_tag="ACHU9J_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1904563.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            988..1908
                     /gene="lpxP"
                     /locus_tag="ACHU9J_000948"
     CDS             988..1908
                     /gene="lpxP"
                     /locus_tag="ACHU9J_000948"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1904564.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2264..2335
                     /gene="ypdK"
                     /locus_tag="ACHU9J_000949"
     CDS             2264..2335
                     /gene="ypdK"
                     /locus_tag="ACHU9J_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1904565.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2400..3638)
                     /gene="alaC"
                     /locus_tag="ACHU9J_000950"
     CDS             complement(2400..3638)
                     /gene="alaC"
                     /locus_tag="ACHU9J_000950"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1904566.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4015..5712
                     /gene="ypdA"
                     /locus_tag="ACHU9J_000951"
     CDS             4015..5712
                     /gene="ypdA"
                     /locus_tag="ACHU9J_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1904567.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5727..6461
                     /gene="ypdB"
                     /locus_tag="ACHU9J_000952"
     CDS             5727..6461
                     /gene="ypdB"
                     /locus_tag="ACHU9J_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1904568.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6474..7331
                     /locus_tag="ACHU9J_000953"
     CDS             6474..7331
                     /locus_tag="ACHU9J_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1904569.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7334..9829)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000954"
                     /pseudo
     CDS             complement(7334..9829)
                     /gene="ptsP"
                     /locus_tag="ACHU9J_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(9854..10891)
                     /gene="ypdE"
                     /locus_tag="ACHU9J_000955"
     CDS             complement(9854..10891)
                     /gene="ypdE"
                     /locus_tag="ACHU9J_000955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1904570.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10891..11976)
                     /gene="ypdF"
                     /locus_tag="ACHU9J_000956"
                     /pseudo
     CDS             complement(10891..11976)
                     /gene="ypdF"
                     /locus_tag="ACHU9J_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11991..13238)
                     /locus_tag="ACHU9J_000957"
     CDS             complement(11991..13238)
                     /locus_tag="ACHU9J_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR1904571.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13260..13586)
                     /locus_tag="ACHU9J_000958"
     CDS             complement(13260..13586)
                     /locus_tag="ACHU9J_000958"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1904572.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13806..14771)
                     /gene="glk"
                     /locus_tag="ACHU9J_000959"
     CDS             complement(13806..14771)
                     /gene="glk"
                     /locus_tag="ACHU9J_000959"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1904573.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14975..16231
                     /locus_tag="ACHU9J_000960"
     CDS             14975..16231
                     /locus_tag="ACHU9J_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1904574.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16346..16672
                     /gene="ypeC"
                     /locus_tag="ACHU9J_000961"
     CDS             16346..16672
                     /gene="ypeC"
                     /locus_tag="ACHU9J_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1904575.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16813..18051)
                     /locus_tag="ACHU9J_000962"
     CDS             complement(16813..18051)
                     /locus_tag="ACHU9J_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1904576.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18387..19589
                     /gene="nupC"
                     /locus_tag="ACHU9J_000963"
     CDS             18387..19589
                     /gene="nupC"
                     /locus_tag="ACHU9J_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1904577.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19639..21827)
                     /locus_tag="ACHU9J_000964"
                     /pseudo
     CDS             complement(19639..21827)
                     /locus_tag="ACHU9J_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(22036..22111)
                     /locus_tag="ACHU9J_000965"
     tRNA            complement(22036..22111)
                     /locus_tag="ACHU9J_000965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22076..22078),aa:Ala,seq:ggc)
     gene            22332..22691
                     /locus_tag="ACHU9J_000966"
     CDS             22332..22691
                     /locus_tag="ACHU9J_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1904578.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22693..23085
                     /locus_tag="ACHU9J_000967"
     CDS             22693..23085
                     /locus_tag="ACHU9J_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1904579.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23137..24552)
                     /gene="gltX"
                     /locus_tag="ACHU9J_000968"
     CDS             complement(23137..24552)
                     /gene="gltX"
                     /locus_tag="ACHU9J_000968"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1904580.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            24811..24886
                     /locus_tag="ACHU9J_000969"
     tRNA            24811..24886
                     /locus_tag="ACHU9J_000969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24844..24846,aa:Val,seq:tac)
     gene            24931..25006
                     /locus_tag="ACHU9J_000970"
     tRNA            24931..25006
                     /locus_tag="ACHU9J_000970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24964..24966,aa:Val,seq:tac)
     gene            25053..25128
                     /locus_tag="ACHU9J_000971"
     tRNA            25053..25128
                     /locus_tag="ACHU9J_000971"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25086..25088,aa:Val,seq:tac)
     gene            25133..25208
                     /locus_tag="ACHU9J_000972"
     tRNA            25133..25208
                     /locus_tag="ACHU9J_000972"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25166..25168,aa:Lys,seq:ttt)
     gene            25239..25364
                     /locus_tag="ACHU9J_000973"
     ncRNA           25239..25364
                     /ncRNA_class="other"
                     /locus_tag="ACHU9J_000973"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            25484..25825
                     /locus_tag="ACHU9J_000974"
     CDS             25484..25825
                     /locus_tag="ACHU9J_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1904581.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(25816..26742)
                     /locus_tag="ACHU9J_000975"
     CDS             complement(25816..26742)
                     /locus_tag="ACHU9J_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1904582.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            26832..27830
                     /locus_tag="ACHU9J_000976"
     CDS             26832..27830
                     /locus_tag="ACHU9J_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1904583.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(27827..28045)
                     /locus_tag="ACHU9J_000977"
     CDS             complement(27827..28045)
                     /locus_tag="ACHU9J_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1904584.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(28047..30062)
                     /gene="ligA"
                     /locus_tag="ACHU9J_000978"
     CDS             complement(28047..30062)
                     /gene="ligA"
                     /locus_tag="ACHU9J_000978"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1904585.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(30133..31119)
                     /gene="zipA"
                     /locus_tag="ACHU9J_000979"
     CDS             complement(30133..31119)
                     /gene="zipA"
                     /locus_tag="ACHU9J_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1904586.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            31349..32110
                     /gene="cysZ"
                     /locus_tag="ACHU9J_000980"
     CDS             31349..32110
                     /gene="cysZ"
                     /locus_tag="ACHU9J_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1904587.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLNVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            32295..33266
                     /gene="cysK"
                     /locus_tag="ACHU9J_000981"
     CDS             32295..33266
                     /gene="cysK"
                     /locus_tag="ACHU9J_000981"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1904588.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            33650..33907
                     /gene="ptsH"
                     /locus_tag="ACHU9J_000982"
     CDS             33650..33907
                     /gene="ptsH"
                     /locus_tag="ACHU9J_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1904589.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            33952..35679
                     /gene="ptsI"
                     /locus_tag="ACHU9J_000983"
     CDS             33952..35679
                     /gene="ptsI"
                     /locus_tag="ACHU9J_000983"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1904590.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            35720..36229
                     /gene="crr"
                     /locus_tag="ACHU9J_000984"
     CDS             35720..36229
                     /gene="crr"
                     /locus_tag="ACHU9J_000984"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1904591.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         8972 a         9511 c         8938 g         8836 t
ORIGIN      
        1 tcgtatggtt ctcagtgaac cattttcagg ataactttct atgaaagtaa acttaatact
       61 tttcagctta tttttattgg tctctattat ggcatgcaat gtttttgcat tttccatttc
      121 tggtggtgta agtgagagga gctataaaga gactgaaaaa acatcagcga tgacaaccac
      181 acactctaca aaacttcagc catcacaggc gattttgttt aagatgagag aagatgcgcc
      241 accattaaac ctcacagaag aaatcacgcc tacttatccg acaaaggcga attatcttat
      301 tcatcctgtg cgatagcgtc tgtcacatgg caggtgaaca atcatgtctg atgctgatgt
      361 gaacacctgc ccaaatgagc catcctgacc cactatcgcc tgccataccc caccataaag
      421 cgaatcatca agccacaagc cggggcaaga aacataaata tccccgtcac cagttcatta
      481 ccgccagtta accacatggt gaagtaataa atgagtgcag cgaggatcac catgccgaag
      541 aaagaagaga ccgccgatac cacgttcagt acctgaccga tgtagccgct tacgcagaca
      601 atacacaggg cgagaaacat ccataaataa atcatagctc aacctcctgc cgcgctaaaa
      661 aacctgtaac ttcagtcact ttaatagtaa gacaatcctt aacctctggc aatttttgct
      721 aagcatcaat gaaaacagca cgttaaatgc aacgctattg cgtgcggaaa attttttact
      781 ttgcacgatt aataatcagt agctgaaagc agtcagccag tgataaagta gagccaccgc
      841 attcatgctc ctcgtggtta tgtcctgact agtctttaca ctctttacag gaaccattgt
      901 cgtacatgat ggcccaacca attgagtgta ccgctgtcga catggaatct tctgcgatac
      961 aacaattcgt atctacagaa ggtaactatg tttccacaat gcaaattttc ccgcgagttt
     1021 ctacatcctc gctactggct cacatggttt gggcttggtg tactctggct ttgggtacag
     1081 cttccttatc ctgttctctg ctttctcggc acgcgtattg gcgcaatggc gcgaccattc
     1141 ctgaaacgtc gtgaatctat cgcccgtaaa aacctggaac tttgtttccc gcagcattct
     1201 gcggaagaac gcgagaagat gattgccgaa aactttcgtt cactcggcat ggcgctggta
     1261 gaaaccggca tggcatggtt ctggcccgac agtcgcgtac gtaaatggtt tgatgttgaa
     1321 gggttggata accctaaacg cgcacaaatg caaaatcgcg gcgtaatggt tgtcggcgtc
     1381 cattttatgt cgctggaact gggcggccgc gtgatgggac tgtgccaacc aatgatggct
     1441 acctatcgtc cacataataa tcagctgatg gaatgggtgc agacccgtgg gcgcatgcgc
     1501 tctaacaaag cgatgatcgg cagaaataat ctgcgcggca ttgtcggtgc actgaagaaa
     1561 ggtgaagcgg tatggtttgc tcccgatcag gattatggtc gtaaaggcag ctccttcgcg
     1621 ccgttctttg cggtggaaaa tgtcgccaca accaatggca cctatgttct ctcccgtctc
     1681 tctggcgcag ccatgttgac cgtaacgatg gtaagaaaag cggattacag cggatatcgt
     1741 ttgttcatca ccccagagat ggaaggctac ccgacagatg aaaatcaagc cgctgcctat
     1801 atgaacaaga ttatcgagaa agagatcatg cgcgcaccgg agcagtacct ctggatccac
     1861 cgtcgcttta aaacgcgccc ggtgggagaa tcgtcgttgt acatttaaca aactgctctt
     1921 actctttccc tcctcaaatc ggccataata acctcatggt ttttatggct tttttcttaa
     1981 ggttttttcc ttcgctgaat atctaattat taccgtcaga aattaaactg tcgccgatat
     2041 gagacacttg taagttacta aaagtaaata agaaaatgcc tcttgtctcg tttcagtttt
     2101 aggcgtacat taacgccgtc ttgagcagtt tagctcagaa ttctggggtg atgcccacgg
     2161 taaaatacag ggcttttaag gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat
     2221 gaagtcattc aggcgtaaca gtaattacgc ggagagatgt aaagtgaaat atttctttat
     2281 gggcatttct tttatggtca tcgtttgggc cggtactttt gccctgatga tctaaaagca
     2341 aactgtgcaa tgaaaaacag gagccatcag gctcctgttt gcatttctga cgacggtgtt
     2401 tattccgcgt tttcgtgaat atgtttgctg ctggcgggta gtaaaccgtc ggcacggaac
     2461 atcgctttaa tccctctgat ggcctgacga atacggtcgc ggttttcaat cagtgcaaag
     2521 cgaacatgcg tatcaccata gtcgccaaag ccaatccccg gcgagacaca aaccttcgct
     2581 tcgttaagga gcttcttggc aaattccagc gatcccatgg ccgcatatgg ttccgggatt
     2641 ttcgcccaga catacatcga agccttcggc atttcgacca tccagcccgc ttcatgcagc
     2701 cctttaacca atacatcacg gcggcgtttg tactgttcgg taatgtcgcg cacgcactgt
     2761 tgatcgccct cgagtgccgc aatcgccgcc acctgcaacg gcgtaaaagt gccgtaatcg
     2821 tgatagcttt tgattcgcgc cagggcactg accagcgttt tgttgccgac cataaagccg
     2881 atacgccagc ccgccatgtt gtagcttttc gacagcgtaa agaactcgac cgccacatcg
     2941 cgtgcacccg gcacctgcat aattgacggc gctttccagc catcgtagac gatatcggca
     3001 taggccaggt catggaccac cagcacatcg tagcgtttcg ccagcgccac aaccttttcg
     3061 aagaactcca gttccacgca ttgcgcggtt gggttcgacg ggaagccgag gatcatcatc
     3121 ttcggtttcg gataactttc acgaatggcg cgctccagtt cgttgaagaa atcgacacct
     3181 tccaccagcg gcactgagcg tacctgcgcc ccggcaatca ccgcgccata aatatggatc
     3241 gggtaacttg gattcggcac cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga
     3301 tgcgccaggc cctctttcga accaatagtg acgatggctt ctgattccgg gtcgatttca
     3361 acgtcgtagc gatcctgata ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt
     3421 gaagtggagt aaccatgcgt gtccgggcgc tgggccacag tacataattt ttcgacgata
     3481 tgcggcggag tcgcaccgtc cgggttaccc atgctgaaat cgataatatc ttcgccgcgc
     3541 cgacgcgcag ccattttcag ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca
     3601 atgcgcgtaa agcgacgttc agggcgagtg tcagccataa tttcctcaga ttaacgttag
     3661 cgcccggacc gtccgagcga cgctgccaca atggtggctg ttttgaaaat agcctgatta
     3721 atttctgtct gtccagcgtt ttagaacatt tttatcaatg gctttaaatg aggaactcca
     3781 cttcaaaaac aggaaatttt tctacgctaa caagaaaatt accaccaatt ttattaacat
     3841 tataatttca ataaatttac cagccagaag tgcattatca agagatacat tgctaataca
     3901 ccatttatct ttcaatcccc ttttaaattt atggtttttc ctcaaaaacg gcctgctggt
     3961 catcagcctt caggttacct atcatagagg tttaatcctt attcagagtc acccgtgcac
     4021 gaaatattca acatgctgct ggcggtcttc gatcgggcag cgttaatgct tatctgcctg
     4081 ttctttctca tccgtatccg cctgtttcgc gaactgttgc acaagtcggc gcactcccca
     4141 aaagaattgc tcgccgttac cgccattttc tcgctgttcg ccctgttcag cacctggtcc
     4201 ggcgttcccg tagaaggctc gctggtgaac gtacgtatta tcgcggtgat gtccgacggg
     4261 attctgtttg gcccgtgggt aggcatcatt accggcgtga ttgcgggtat tcaccggtat
     4321 ttaattgata tcggcggcgt gacggcgatc ccctgcttta tcaccagcat tctggcgggt
     4381 tgtatatcag gctggataaa cctgaaaatc cccaaagcac agcgctggcg cgtcggtatt
     4441 ctcggcggca tgttgtgtga gacgttgacc atgattctgg taattgtctg ggcaccaact
     4501 accgcgttgg ggatcgatat cgtctctaaa atcggcattc caatgatcct cggtagcgtc
     4561 tgtatcggct ttattgtgct tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc
     4621 gcgcggcagg ccaagctggc gctggatatc gccaacaaaa cgctaccgct gtttcgccat
     4681 gtcaatagcg agtcattacg caaggtctgc gaaattatcc gcgatgacat tcacgccgat
     4741 gcggtggcga ttactaatac cgatcatgtg ctggcctatg ttggcgtggg tgaacataac
     4801 tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa ttacggaaaa
     4861 atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc catgctggtg
     4921 atcccattgt gggagaaagg ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg
     4981 catcagatca cctcgtcatt acaggaaatg gcggtcggtc tgtcgcaaat catctccacg
     5041 caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc agagcttcgc
     5101 gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg ctctgaacgc tatttcatcg
     5161 tcaatccgtc tgaatccgga taccgctcgc cagttgatct ttaatctgtc gcgttatctg
     5221 cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga gctgtatcaa
     5281 attaaagact atattgccat tgagcaggcc cgctttggtg acaagctgac ggttatctat
     5341 gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc gttggtggag
     5401 aacgccattg tccacggtat tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt
     5461 gcagagtgcg gaaatcgggt acgcattgcg gtgcgagata ccgggcacgg catcgatcca
     5521 aaggtgattg agcgggtcga agcgaatgaa atgccgggca ataaaattgg cctgctgaat
     5581 gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg cctggagccg
     5641 gggacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc acaggctacg
     5701 ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac gaattcctgg
     5761 cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt gtcggcacct
     5821 ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc
     5881 tggatatcaa tattccgtcg ctggatggcg tgttgctggc gcaaaacatc agccagttcg
     5941 cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg
     6001 aactggaggc gtttgactac attctcaaac cgtatcagga gtcacgtatt accgggatgc
     6061 tgcaaaaact ggaagcggcc tggcaacaac agcagaccag cagtacgcct gccgcgacgg
     6121 taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc gtcacgccaa
     6181 ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg
     6241 aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg ccgtcgcatt
     6301 ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt
     6361 ggtttaataa cacctacatt ctgcgactga aagatctgga ttttgaagtg ccggtcagcc
     6421 gcagcaaagt gaaagaattt cgccagttaa tgcatcttta atcgggaact ttcatgaaag
     6481 caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc ctggtgctta
     6541 acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgtta ccggtgggca
     6601 gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc
     6661 tggaagcaaa gcagcagcga atggtggaag gagaaatgct gtttattccg gcacgcgcgg
     6721 ctaatttacc gatcaacaac aaaccggtga tgctgttaag cctggtgttc gcaccgacct
     6781 ggcttgggtt atcgttttac gatagccgca ccacgtcgtt gttgcatcct gctcgccaga
     6841 tccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc
     6901 ttagccgttc gccgctggag cagaatatca ttcagccact ggtgttaagt ttgctgcatc
     6961 tttgccgcaa cgtggtgaat atgccgccgg gtaattcgca accgcgcggc gattttctct
     7021 atcacagcat ttgtaactgg gttcaggata attatgccca gccgctcacc cgcgagagcg
     7081 tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct cagcatggaa
     7141 cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc
     7201 agaaatatca tctgtcaatt catgaagtgg cacagcgttg cggttttccg gatagcgact
     7261 atttttgtcg cgttttccgg cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt
     7321 ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata ctttgtgcat
     7381 cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc gccagctgcg
     7441 agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg atcaccagtt
     7501 caacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca atgctgatac
     7561 tggaatgacg gatccactga gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg
     7621 tggtaacaat ctcttcccgc tgccagacat cttcttccag ctcaaacgga tgttcagtgc
     7681 gcccgttaac gccgaggttg ccgcacagga actggatcgc ctgctcttta ttgctaaaat
     7741 cctgatcaac aaagatattc tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa
     7801 aggcggtgag taacgcttca atttcctgcg cactgcggca ttcacatgcc tgacgcgcca
     7861 gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc ggaatacgcg
     7921 ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga taacggcttt
     7981 caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca gtggtaacta
     8041 tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc gatacgcgcg
     8101 ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg atactgaaga
     8161 aatcgacctc atcggagaag tggtcgatga tgtagcacac cgacggcact tccaccatga
     8221 tcccaagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca acgatcgctt
     8281 tttggatctc gcctttcacc cataagatct gatcgaggct gtgaaccatc gggatcatca
     8341 actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg cggaacaggc
     8401 cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaacgggttc tcttcctggg
     8461 gaatattcag ataaggaatg cttttatcgc cgccgatgtc catcgtgcgg aagataatcg
     8521 gcttgtcgcc cgccgccagt agcacctgct ggtaggcttc aaattgctcc tgctcgtccg
     8581 gcgcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgaca ccttccgcgc
     8641 cgttggcaaa cgcgcctggc gcttccagag cggtgccgat attcgccgca atatcaatac
     8701 gcttgttatc acgggaatag gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt
     8761 tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc gggttaatcg
     8821 ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgg gcaatcgcat
     8881 ccagcggcaa gccactcagt accgggatcg ccgaggcacg ggccagaatc agtgtatgcg
     8941 aggtgcgccc ggttttctcc agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa
     9001 actggcttgg ggttaaatct tcagccacca gaatggtcgg tttttccagc accagattgt
     9061 tgcgcggctt cagttccggc taggtgatat gcagcaactg ttcgctgata tcgcgaatgt
     9121 cgctgacccg ctcgcgcaga taatcgctgg cagaagcaga tagtttggcg caaacctgct
     9181 ccatattgct gatgatcgcc gcccccagtc cctgatgctg ttctgtcatc aggcgacgga
     9241 tattgcctgc aaattcatca tcctgaatca gcgacaaatg agcgctgagg atagttttgc
     9301 tttcgccgtc acgctcacgc aattgctggt tcagttgctc ggcaagcgtt gccaggctgt
     9361 gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg tcgaggctgt
     9421 cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca ttgccgtaca
     9481 gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc agttctgcct
     9541 gcgtcggctg aacgctgtcg ctgtcgataa agcgcaccag gatgtactct tccagcacgc
     9601 gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca ttaaataagg
     9661 tgccagttcc aatcaacgcc agcgagcttt tagcatcggc ctttgcgttc tggcgatggt
     9721 tgataaaggt gatttcgctt tgccactggc tgcactgttc tttaagttcc cacgccggac
     9781 gagcatgtag accgttaggc aaaggacaga gaaattgaat cgttaacata accgctccgg
     9841 gttaatcagg agatcatctg aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt
     9901 gaataagggc agataaaagt tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg
     9961 ccgcgcaatg tccgtggcgg gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg
    10021 ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa catatccgcc tgcaacggaa
    10081 cgccaatttc tgccgacacg gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg
    10141 atttatcgct taacaccagc atcggcccgt taccaatctg acgatggtta gccgcgccat
    10201 aatcaaagtt tttcgcccag caggcggtat caagcacaat ggcgacgtcc ggcgacaccg
    10261 cgcgggtagc agtttgcccg ccgcgtaatc ccacctcttc gctggaactt gccaccagcc
    10321 acacttccgc aggcagttcg gcgtcgtgca gctcacgtag taacgtcacc agcagatagc
    10381 aaccgaggcg gtcatcaaag gctttcccca tcacccgctg gtgagggaga acctgaaaag
    10441 ttgtatcaaa ggtgacgcga tcgcctggac gaattcccgc ctgcatcact tcgtcatacg
    10501 agcgcgcacc aatgtccacg cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa
    10561 gcaggcctgg aattttgcac tcttcacggg tggtgatgcg caccggctgc agctggcggg
    10621 cagccatgcg cacgttgcca accggcagca catcaattgc cccttcgcgg gagatgctgc
    10681 gcaccataaa tcccacttcg tccatatgcg cacagatcat caccttcgga cctgtcgatt
    10741 cattgaggcg gatcagcacc gatcccagac catcaaatcg tacttctttt tgcaggcgat
    10801 ccgcttcttc cagtaggatc tgccgcactt cctgttccga ggaggcgatc gcatctgcct
    10861 cgctcaacgc ttttaatagc gataaatcca ttatgcctct cccgtgagca acactgtttt
    10921 cggcatggcg tagagcactt ctgcgccttg cggggtaacc agcacaacat cttcgatgcg
    10981 cacgccccct tgccctggca aataaatccc cggctccacg gtcagtaaca tgcctggctg
    11041 tagcgtcgtg gtatcccgcg gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc
    11101 atgaccggtg ttgtgaccaa aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc
    11161 ggcgtcgtca acctgttagc agcgtacgcc agggcgaatc gcggagattg ccgccagctg
    11221 tgcttgcagg acaatctgat agacgttaaa cagcgggtga gattcggcaa ttaccccttc
    11281 gccattcacc agcaaggtgc gcgtcatatc agagcagtac ccctgataga gcgcaccaaa
    11341 atcaagagtg acaaactcgc ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag
    11401 cgccccacgc cagccgctgg cgacaatggt gtcaaaagag gctttttctg cgccctgctg
    11461 gcgcataaac cactccagtt cagcggctat ctcgcgctcg ctcatccccg cctgaataaa
    11521 gcggcgaata tgctccgctt cgcgatcggc aatcccacag gcgaggcgga ttatctccac
    11581 ctcctctggc gttttgattt gccgcagcac atccggcgtg gcgctaacca gtttcgcatt
    11641 gagttcagac ttccagcggt gcgcggtttc ccaactcacc tgctggcctt caaaaccgag
    11701 cgtttgcaac tgctcatcgg caatgatttg attgacgata gtggtaagcg tgttcgtcgc
    11761 gtcaagcaaa tgcagctggt agccttgcgc acgggcttcc acctcaacga aatagcgcga
    11821 atctaccaga atgtgcgcac tttcacggct aataaccaca tagcctgatc cggtggagat
    11881 ccccagatgc ggctgtttgt tctgccgtga ggagagaagc actgcatcca gttgttgcgc
    11941 cttaagccag tcgcgcagcg aagcgagtaa tgtcatctgg attttccttc ttacaggcta
    12001 tcgattaaca atttaccttt acggaacatc atcagacgca ggaacaccac catcagcgca
    12061 gttatgactg cccccagcgc gatccccgcc atatagacgc ccaggttagt gaccagcggc
    12121 catgcccaaa tagcggattc cgggaaccat tgcactgcgc ccagccagac agcggcggtt
    12181 gagccgacaa tcgcgccgac catatacgac ggaatggcgg tgatggggct ttccagcgca
    12241 aacggaatcg caccctcact aatccccata aaggcaagga acatcgcggt cttaccctgc
    12301 ggataaagtt gagcgttgaa caggcgtttg ccggttaaac gacggtcgat aatggtcgcc
    12361 agacccagac ctatcggcgg aataacgata gcgatagaac gtgcagtgac cggtaatacg
    12421 tggtcagtgg taaagctgaa ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg
    12481 tcgattgctg tcgccgcggc gatccccatt gcgtacatca gcgcgccctt ctcacctgcg
    12541 gcagtcagca cggtacggat accgccgttg atccagccgc caaagggggt aatgacgtag
    12601 tacatcgcca gcatgacaaa aatggcggaa agaatcggta gcaaaaatgt ggttttgaac
    12661 gctaacagga aatccggtaa ctggattttc tggttcatcc acttcacaag gtaacccgcg
    12721 acgatagaaa taatcagcgc accaatgaag gtggacggca ccggcgaaga ggtcgcccat
    12781 tgcatagtgc tgggatcgaa gttcagcagt tgcgtcggct gggtagacat caagccgccg
    12841 ataaaaccag ccgggaacgc cagtttgcca ccgatagagt tcgccacaaa agcagcaaac
    12901 atcggaatgg caaaaccaaa caacacgccg ccgaaggact gcgacagcca ggcaaatttc
    12961 agtaaggaga ggtcgaaacc ggagaatttc ccgctattaa gagcatccat gatgccgatc
    13021 tcagcaggaa ttttaagcca gctataagca atcagctggc tgaaagcgag gatcacaccg
    13081 cccataatta aggtcggcac catgcgtgaa atccctaaca tcacatgctg cgggagttca
    13141 ccccagaagc tgcttttaga ggcctgcgga ttcttaactg ccgctgccgc accggatgcg
    13201 ccaggcacaa cggttgcact gcgtttttta atggccataa caattatccc tgtcgaatat
    13261 tactgttgtt cagaggcaat catctcttcg atttctttga tgatgcccgc agcgttttta
    13321 attgcgtcct gcaaagtgat ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc
    13381 ggggtaactg ccacggagtg gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg
    13441 cgattctgga taccgtccgc gccctgggtt tcaattttca cttcataacc tgcttctacc
    13501 gccgcttctt ccagcgcctg agcggccata aaggtgtgag ccaggcccat tgggcaggca
    13561 cataaggcaa ttagtttctt actcatcgtt aattcctcgt tcagtgatag ttgacgagtg
    13621 aaattatgaa cgtgcaaaac aaatcgccgt taccagacaa ataatgcatt tactggagaa
    13681 ttaatcccgc tgtacggaag tgtgacgcag gtcggccttg tgagggggac acaaagaaaa
    13741 attgaattaa ctgtatgatt taaaagatta tcgggagagt tacctcccga tataacagaa
    13801 aggatttaca gaatgtgccc taaggtctgg cgtaaatgtg caccggaacc gagaagaccc
    13861 ggattgtcat ggacgatgag atacaccgga atatcatgga catattcttt aaagcgccct
    13921 ttatcttcaa atgcggcacg gaaaccagag gctttgaaga actcaaggaa gcgcggcacg
    13981 ataccgccag caatataaac cccaccaaag gttcccagcg ttaatgccag attgccgcca
    14041 aaacggccca taatgacgca aaacagcgac aacgcacggc ggcaatcggt gcagctgtca
    14101 gccagcgcgc gttcggtaat atcttttggc ttgagatttt ctggcaggcg gttgtcggct
    14161 ttaacaattg cgcgatacaa attcaccagc ccaggaccag aaagcacgcg ttccgccgaa
    14221 acatgaccaa tttccgcacg caatatttcg aggataatgg cctcttcttc actattcggc
    14281 gcaaaatcaa cgtgaccgcc ttcgcccggc aagcttaccc aacgcttatc gacatggacc
    14341 agatgcgcaa ccccaagccc tgttccggca ccgtaaaccg caataggttt accttcgacc
    14401 ggttctgcgc caccaaactg aatcagatgc tcttttttca gcatcgggat cgccatcgat
    14461 acagcggtaa aatcgttaat aatttccaga tggctaaaac cgagattctt tttcatttcg
    14521 gcaattgaga acgcccaggt atggttggtc attgccaccc agtcaccggt aattgggcaa
    14581 gcgatggcaa tacagccgtc tttcacctcg accttatgtt cttcaagata aacgcgaatg
    14641 accgcttcga ggctggggta atcaagccct gaataggtct tagcctgcga gatttcacca
    14701 ctggcaatat cacacagagc aagacgtgcg ttggtgccgc ccacatcacc gactaatgca
    14761 tactttgtca ttcttcaact gctccgctaa agtcaaaata attctttctc acactgtaaa
    14821 tacctggggg cataacaaca acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt
    14881 cacacattta ctttatcgtt tcagcaccaa ttgcagcgat gcctttttgc aagctgggca
    14941 aactaagtat ctgaccccgc ataaggaata gaacatgctc catccgcgag ccagaaccat
    15001 gttgttatta tcgctccccg ccgtggcaat tgggattgcg tccagtctta ttctgattgt
    15061 ggtgatgaaa atcgcctcgg tattacagaa tttgctctgg caacgactgc cgggaactct
    15121 ggggatagcc caggattcac ctatctggat catcggtgta ttaacgctaa cgggtattgc
    15181 ggtggggttg gttatccgtt tcagccaggg tcatgccgga ccagaccccg cctgtgaacc
    15241 gctgatcggc gcaccagttc cgccctctgc gctaccagga cttatcgtag cattaattct
    15301 cggtcttgct ggcggcgtca gcctggggcc ggaacatccg atcatgaccg tcaatatcgc
    15361 ccttgccgtc gccattggcg ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat
    15421 tttagcctct gccggaacca tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat
    15481 attttcgcaa accttaaatg gcagtagtga agttccgcta tgggatcgtc tttttgcgcc
    15541 gttaatggcg gcagcagctg gtgcacttac taccggatta tttttccatc cccatttttc
    15601 actgcccatt gcgcattacg gacagatgga aatgaccgat attctcagcg gtgcaattgt
    15661 cgcggcgatt gccatcgcag cagggatggt cgccgtatgg tgcttaccac ggttgcacgc
    15721 gatgatgaat caaatgaaaa atccggtgct cgtgctgggt attggcggat ttattctcgg
    15781 aattctgggg gttattggtg gaccagtttc gctgtttaaa gggctggatg agatgcagca
    15841 gatggtggca aatcaggctt tcagcaccag cgattacttt ttgctggcgg taattaaact
    15901 tgccgccctg gtcgttgctg ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt
    15961 gtttgtcggc gtggcattag ggttgatgct gcatgagcac gttcccgccg ttccagcggc
    16021 aataaccgtt tcctgcgcta ttctcggcat cgtgctggtg gtaacacgcg atggctggtt
    16081 aagtcttttt atggcggcag tcgttgtacc caataccaca ttgctaccgc tgctctgtat
    16141 cgtcatgctt ccggcatggc tgttattagc aggtaagccg atgatgatgg tcaatcgtcc
    16201 gaagcaacag ccaccccacg ataatgttta gcaaaaaatg ctctccttta tgatttaaga
    16261 gttatggcga tgatacgggg ctttacctcc ccgtaatatt gcgttaacag gccgctgacg
    16321 gcttcgatcg tgaaggagaa taacaatgtt caggtcactg tttctggcgg ccgccctgat
    16381 ggcatttacc ccgcttgcag caaatgcagg tgaaatcacc ctactgccat cgatcaaatt
    16441 acaaattggc gatcgcgatc attacggtaa ttactgggac ggtggtcact ggcgagatcg
    16501 tgattactgg catcgcaatt atgaatggcg caaaaaccgc tggtggcgtc atgataatgg
    16561 ctatcatcgt ggctgggata agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg
    16621 cgatcgtgac gatcatcgcg gaaaaggccg cggacatggg caccgccatt aaacgcgtcc
    16681 tccaatggag cacaatgcct gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc
    16741 tctttaacgc gtagggcgga taaggcgttt acgccgcatc cgccaatagt gccagatgcg
    16801 acgctcattc aactacaatc ccagcgccgt ccccaccaac aaccagatat tcagcgccac
    16861 gaccagcact acaatcaccc agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc
    16921 catcaacttg ctgtcactgg tgaaaatcag cagtggaacc agcgccaggg cgataccaaa
    16981 acttaacagc acctgactca taaccagaat ccgtgtcgga tctaatccca tcagaatgac
    17041 aataaatgac ggcaacatgg tgactgtacg acgcacccac agcgggatat ggaagcgaat
    17101 gaatccctgc atcaccacct gccccgccag tgtccccacc accgttgagg acagtccggc
    17161 agcaaccaga cttaacccaa agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt
    17221 cagataagcc tcatcaagat cggcaacacc agtatgacca gaaaagtgga acgccgccgc
    17281 agctgtagcc atcatcgcca gattgacaaa accggcaatc gtcatggcga tagccacatc
    17341 ccatttggtg gcggaataac gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga
    17401 agagtgcaaa taaatcacat gcggcataat cgtcgcccct aacacgcctg ctgccaggaa
    17461 gaccgcctcc gaagtaggta aactcgggat caccattcct ttacccagct gcgccaggtt
    17521 aggctgggag aaaatcaact cgacaatgta agccgcggca acaaacaaca gtaacccgcc
    17581 aatcactttc tccagtggtt tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc
    17641 gatccccgtc agcaccgcgc cctgcaacaa cgagacacca agaatgagtt taaaaccgat
    17701 cgccgcacca ataaattccg ccaggtcggt tgccatcgca ataatttctg cctgaaccca
    17761 atagaaccac actacgggac gcggatagtg atcacgaatc tgctccgcca gattttttcc
    17821 ggtggcaatc cctagtttgg cagagaggat ctgaatcagc atcgccatca ggttggccca
    17881 aacgacaacc cacagtagct gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa
    17941 gttaccggga tcgatataac caatcgccgc aatgaacgca ggtcccatta atgcgagcct
    18001 catcttgcgc gccgcccgtc cgctgctact ctcaacgcga tagttcgtca tcttgtgcct
    18061 ctaaaacata gcctttgcta tgtttcatgc tatgataaac gagaatgatt atcaaattca
    18121 tttaaatgga ttgtggtgat ttctctgata gaccaggatt atgactacga aaagattgat
    18181 gacttgaatg ttatggttat gtttaatgtt agcacattta cataactttc agcttccata
    18241 cacaacatag cagaaatgta tgacagatca ctatttttga agcctgtcac aggacgtcat
    18301 tatagtgtgt gtcagatctc gttttcctta accatgttac atagaatgtg cacggaaatt
    18361 taacctgcct catatttgga gcaaatatgg accgcgtcct tcattttgta ctggcacttg
    18421 ccgttgttgc gattctcgca ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt
    18481 atgttattca actgcttgtt atcgaagtgt tactggcgtg gttcttcctg aactccgacg
    18541 ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga aaaactgctc agatttgcca
    18601 acgaagggac taacttcgtc tttggtagca tgaatgatca aggcctggca ttcttcttcc
    18661 tgaaagtgct gtgcccaatc gtctttatct ctgcactgat cggtattctc cagcacattc
    18721 gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg
    18781 gcaaactgga atcctttaac gccgtcagct ccctgattct gggtcagtct gaaaacttta
    18841 ttgcctataa agatatcctc ggcaaaatct cccgtaatcg tatgtacacc atggctgcca
    18901 cggcaatgtc caccgtgtcg atgtccatcg ttggtgcata catgaccatg ctggaaccga
    18961 aatacgtcgt tgctgcgctg gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga
    19021 tcaatcctta ccgtgttgat gccagtgaag aaaacatcca gatgtccaac ctgcacgaag
    19081 gtcagagctt cttcgaaatg ctgggtgaat acattctggc aggtttcaaa gttgccatta
    19141 tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg
    19201 ccaccgttac tggctggttt ggctacagca tctccttcca gggcatcctg ggctacatct
    19261 tctatccgat tgcatgggtg atgggtgttc cttccagtga agcactgcaa gtgggcagta
    19321 tcatggcgac caaactggtt tccaacgagt tcgttgcgat gatggatctg cagaaaattg
    19381 cttccacgct ctctccgcgt gctgaaggca tcatctctgt gttcctggtt tccttcgcta
    19441 acttctcttc aatcgggatt atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta
    19501 acgtggtttc tcgcttcggt ctgaaactgg tttacggctc taccctggtg agtgtgctgt
    19561 ctgcgtcaat cgcagcactg gtgctgtaaa accatacata aaaaagccgg ggatcattcc
    19621 ccggcttttt tacgcctgtt aatcagctaa tggctgcggg cgaccaatca aatacccttg
    19681 cagatattgc accccgagct tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc
    19741 cgcgaccaca ctcaatgact tcgctttcgc cagatcggta attgatctca caatcatcgc
    19801 atccagcgta ttcgtgacaa tatctttcac aaagacgcca tcaattttga tgatatcagc
    19861 ctgcaaacgc tttaaccgtt cgtagttggc atatccggtg ccaaagtcat caatcgcaat
    19921 ccggaaacca aacttatgca gctgctcgat gttgtacatg ctgctttctg cgttagaaaa
    19981 cgcctgctcc tcggtgatct caagaatgac cgcctgcggg gagatgtgat aacgcttaaa
    20041 cagacgaata atccgcccgg caatattctt ttgcagcagc gtgagcggca ttaaattgac
    20101 tgaaaagcgc ggtccttttt tgtcgcaagg gtgtgttgcc agccacttca acaaggattc
    20161 cagcacttgc aaatcaaaac gcgcgctaag gttaaactga gcaataaggg gcagaaactt
    20221 atccggggtc ataatgccgc cgtcatattt cagtcgcgcg aggatctcat cataaccttc
    20281 accctctttg ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt
    20341 gcgaatggta ttcagcaata gcacctgttt agtggtctgc ccggaaacca tctcctctct
    20401 gctatccagc gccagcacat gatgatgtgc gcaggattgc tccgccagcc agcttaactg
    20461 ccccaacaag ggttgcaggg tttcctgatt tccatcaaaa cgcccccagg cagcaccata
    20521 gcccatatcc agcccggtat tgttccagtg aatttgccga ctattcagga tgttaaccat
    20581 atgctggagt cgcccttccg tttccggccc gctcagcact aacagcagtt cactacccgg
    20641 caattgatat aacttttcgt tttcctgcat caacggcagc agcgtacggt aaattgatcg
    20701 gatacagtga acgcgcatca ttaagccgta atgacgactc ataaactcaa gattatcaat
    20761 gcgcaggcag caaaactctt gcccgcctct tgctccggcg cttgttccaa cgcacggaag
    20821 ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg attaagccat
    20881 tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga aatcagcacc
    20941 gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt ctggttgtaa
    21001 ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagctta
    21061 ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc aataaagtcg
    21121 ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag ccagcttaag
    21181 gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca caatatctgc
    21241 gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt gtaaataagc
    21301 acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa
    21361 aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat gctgcatttg
    21421 atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata tcggctggtc
    21481 aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc acacaacacg
    21541 acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg aaagttccac
    21601 agattggtgc aaaacatccc gcataaaacg ggaacgacac cgcgccagcc aaagataaac
    21661 aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact ggaatggata
    21721 atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag cgtaaacgct
    21781 agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc
    21841 tcactgatcg gggttttcag gcacacaaat aataagttta ttcgcatcgc gaagcaaaca
    21901 gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg gcactaaccc
    21961 ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc cgctttgcag
    22021 cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    22081 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    22141 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    22201 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    22261 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    22321 agggaattgt tatgttcaag gagaggatga caccagatga acttgccaga ctgaccggct
    22381 atagccgcca gaccattaat aaatgggtac gcaaggaggg ctggacaacg tcaccaaaac
    22441 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    22501 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    22561 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    22621 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    22681 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    22741 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
    22801 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    22861 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    22921 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    22981 atagataata tgacagacat tgagcaggga aaagtcgcgc aatttttgtc acgtgaagga
    23041 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    23101 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    23161 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    23221 ggtaacatcc agtgctggag actgccccgc cccggttacg gcgacacgca gcggcatacc
    23281 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    23341 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    23401 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    23461 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    23521 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    23581 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    23641 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    23701 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    23761 cagatagttc agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    23821 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    23881 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    23941 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    24001 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    24061 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    24121 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    24181 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    24241 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    24301 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    24361 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    24421 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    24481 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    24541 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    24601 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    24661 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    24721 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    24781 ctctccctat aatgcggctc cacacagcgg gggtgattag ctcagctggg agagcacctc
    24841 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    24901 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    24961 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaattt ctcgccagct
    25021 aaatttcttg taaaaatgtg aagtaccgaa gtgggtgatt agctcagctg ggagagcacc
    25081 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccact tcgggtcgtt
    25141 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    25201 acccaccaat gtaaaaaagc gccctaaagg cgcttttttg ctatctgcga tactcaaaga
    25261 ttcgaacctg cagcaggttt gagttgagcg cagcgaaaca acggagccgc tcgcggcgac
    25321 ggcccgaagg gcgagcgaag cgagtcatcc tgcacgaccc accaatgtaa aaaagcgccc
    25381 taaaggcgct tttttgctat ctgcgatttg taagattgat ttttcttctc aagttccttg
    25441 ttcttctgcc gatattacct cgtacagtga cgaggactga acaatggcca ctatctcttc
    25501 aacttcaatt ccttccatac aaacacagag tagtaacagg gcatcacaag gtagcgatgt
    25561 tgcttcaaaa atcgcgcgga taagtcagca aatcatcaaa ctcactcaac aaattaaaga
    25621 gattgtagac actagcggca gtgctgaaga caaacaaaaa caggccgaat tgattcaaca
    25681 gcaaatcacc ctactggaaa cacaactggc gcagttgcag agacagcagg cagaaaaggc
    25741 gcaggagaaa gagcagcgtt tgtctctgaa tgtatccctt ctaaatcctg ttgaaaatac
    25801 gactcatatc gatatctata tctgatacaa cggagcccct tcccaaccag ccattagcgc
    25861 attgccctgg tctcgtacca cgtcccataa cgcttcagcg gcggtggaaa gtgaacggtt
    25921 cttcctgcgc accagcatca actggcgttc aacaaccggc gtaatgcgct ttaccaccag
    25981 tgggctacct tcaggcaatg gtagcgccag tgcgggaaga atactgatgc cgatgcctgc
    26041 ggctaccatc gggaacagcg tcgccggatg tccaatctcc tgcacaatat tcgcctgaat
    26101 accattgcgc gccagcgctg cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa
    26161 caccaatttc gctccctgta atgcctgcca gggtacataa tcttccacgg ccaaagcgct
    26221 atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc
    26281 cactgggcct ggatcgatga cgatgcaaaa atcgacatct ccctggcgaa tactttccat
    26341 cacccactgc tgcggacgat cgtgcaggac aaactgaata tctggatagc ggcgatggct
    26401 ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc tggctggcag cgacccgaac
    26461 ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc
    26521 cagcagtcgc tcaagacgca aagccaactg ctgccctgca tctgtaagca ccacttcacg
    26581 cgtggttctg tccagcaggc gaacaccagt atgattttcc agctccttca cactgtgact
    26641 tactgccgac tggctcaagc cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc
    26701 tactgtgacg aaaaccttta attgttttaa agaataattc atctgttttc ttcatgaatg
    26761 gatgcaataa atcaatttta tttctcaaac tggaagaagc acaatagaac catcgatcat
    26821 ctggagtctt tatgaaactt tttcgtatcc tcgatccttt caccttaacc ctgatcacgg
    26881 tggtgttgct ggcctctttc tttccggcca gaggcgattt cgtccccttc tttgaaaatc
    26941 tgaccaccgc agctattgcc ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgagg
    27001 cgattattgc tggcggtggt cactggcgac tgcatttgtg ggtaatgtgc agcaccttcg
    27061 tgctgtttcc gattctgggt gtactgtttg cctggtggaa accggtaaat gtcgacccga
    27121 tgctctactc cggttttctc tacttgtgca ttctcccggc taccgtgcag tctgcaatcg
    27181 ccttcacgtc aatggcgggc ggtaacgtcg cggcggcggt ttgttctgcg tcagcatcca
    27241 gcctgctggg gattttcctt tcaccattgc tagttggtct ggtgatgaat gttcacggtg
    27301 cagggggcag ccttgagcag gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt
    27361 tggggcatct ttcccggccg tggattggtg actgggtgtc gcgcaataaa aaatggattg
    27421 cgaaaactga ccagacgtcc attctgttgg tggtttatac agcgttcagc gaagccgtcg
    27481 ttaatggtat ctggcataaa gttggctggg gatcattgct gtttatcgtg gtggtcagct
    27541 gcgttcttct ggctatcgtg attgtagtta acgtctttat ggcacgccga ctgggcttca
    27601 ataaggcaga tgaaattact atcgtctttt gtggttcgaa aaagagtctg gcaaatggca
    27661 tcccgatggc aaacattctg ttccccacat cggtgatcgg tatgatggtg ctgcccctga
    27721 tgattttcca tcagatccaa ttgatggtct gtgcggtgct ggcgcgtcga tacaaacgcc
    27781 agaccgaaca gttacaggcg cagcaggaaa gcagcgccga taaagcttaa agcggacgct
    27841 tcaggggctg gaccagttgc gtcagcccct cggttttaat cagcagcgtg atttgcatta
    27901 gctcaccgag cttccctgcc gggaactcat ctttgcgggc aaaccacagc agatactcct
    27961 ccggcaggtc gattaagcga cgccctttgt atttgccaaa cggcattatc gtattggcta
    28021 tttcaatcag ctgctctttt tccatctcag ctacccagca aacgcagcat ttccgcttcg
    28081 tcgatgactt caatgcccag ttcctgcgcc ttcgccagtt tagatcctgc agcttcaccc
    28141 gctatcacca gatcggtttt cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc
    28201 agtcgagctt tagcgtcatc acgcgacatc tggcttaagc tgcccgtaag caccacggtt
    28261 ttaccagcaa acgggctgtc aatctcttcc gcgttgataa cgatcggcgc aggccagtga
    28321 acaccttccg ccaacagctc gctgatgaca ttgcggttgc tttcttcggc aaagaagttg
    28381 tgaacgtggg atgcaacgac aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa
    28441 gcggcttcca gcacttccag cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg
    28501 ccgacttcac ggatgccaag tgcatagagg aagcgagcaa aggtggtttc tttcgctttt
    28561 tccagcgcgt taaccacgtt ttgtgccgat tttggcccca tacgctccag tccggtcagt
    28621 ttgcctgcgg tgagtttgaa cagatctgcc ggagtgtgga catattcttt ttcaaccagc
    28681 tgatcgatga ttttgtcgcc cattccgtca acatccatcg cacggcggga aacaaagtgt
    28741 ttcagcgact ctttacgctg cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct
    28801 tcaccttcca cacgctcaac gtcagaacca cataccggac aatgcgtcgg gaatacaacc
    28861 tcacgggtat cttccgggcg ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg
    28921 ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac caagacgttc gatttcatcc
    28981 gcattgtgta aggttgcgtt actcaccagc acgccagcaa catggacagg ttccagacgc
    29041 gcaacaggcg taatcgcgcc agtacgccca acctgaaact cgacgtcacg cacaaaggtc
    29101 atctgctcct gcgccgggaa tttaaacgct accgcccagc gcggggcacg cgcgacaaag
    29161 ccaagctgct cctgctgttc cagtgagttg accttaatca ccacgccgtc gatatcaaag
    29221 cccagcgtcg ggcggtcttc ttccactttg tgatagaacg ccagcacttc ttccgccgat
    29281 tcacaaagcg ttacccgatc gctgaccggc aacccccact ttttaaattg cagtaaacgg
    29341 ccaagatgag tatccggcag ctcgccacct tccagaacac caacgccata gcagaaaaaa
    29401 gtgagcggtc gcttcgctgt aatacgcgga tcaagctgac gcagtgaacc agctgccgca
    29461 ttacgtgggt tagcaaaaac tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg
    29521 aaccccgcct gcggcaggaa cacttcacca cgcacttcca gacgcgccgg gatattctct
    29581 ccgtgcagct tcagcggaat ggcgcgaata gtacgcacat tagacgtgat atcttccccg
    29641 gtggtgccat cgccacgggt cgcggcactg actaaaacgc cattttcata cagaatactg
    29701 acggcaaggc catccagctt cagctcacag caccaggtga ctttctcgtt gtttttcaga
    29761 cggtcctgca cacgtttgtt gaaagcaaga aagctttctt catcaaaaac gttatccagt
    29821 gacagcattg gtacttcatg gcgtatctgg ctgaaagccg ccagcggcgc agcacctaca
    29881 cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt
    29941 tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg gcgcatccat cacatgataa
    30001 agatattcat gatggcgaag cgtcgttcgc agttctgtca gttgttgttc gattgattcc
    30061 atatcgcacc atcaatgcta aaaacccccg acaagcgggg gttcgaagag gagttaattt
    30121 gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg
    30181 caatttctgc ggagtcatca tacggcgctg atcgtcaagc acgacaccgc ccacttcatc
    30241 ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc tgcagctcgt caccgtaaga
    30301 cggtacctgc ataaagatag tgacacccgg agtagtgaaa tccttcattt caggatcaaa
    30361 ggttcccggt ttcaccatat tcgccaggct gaataacgcc gggccgctgc catccgggct
    30421 aagatgacga tggtaaatat tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt
    30481 aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc gcgacgttca tgataatcac
    30541 cgcttctttg cgcttcggtt tatccataac tggagccggt tccgctacag gctcaggctg
    30601 tggtgccgct acgggttctg caggctggaa agcctgttgt gccggttgcg gtgctgaatg
    30661 cacaggctgc ggcgctggcg cgacctgtgg cgaaactggc tgctgcaacg gctgttcagg
    30721 ctgcggctga taggcaggct gctgcatcgg ctgcgctgga cgcggagcat gttgcggcgg
    30781 tacctgcgct tcaggcggct gctggatcgg ttgacgcggc tgcgcagacg cataaggcgg
    30841 ttggtactgg tgttgcggcg acggacgagc agcctcatgc tcctgagcgt tagccggggc
    30901 atgattcacg cggtgaacac gaacctcacc aacgccctca tcatcttcga catcctcgtc
    30961 ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat ggccgatcgc ggaacataga
    31021 agatcgttct ttacggctgg tccagaaacc atgtaccagt aaagcgatta tggcgatcgc
    31081 gccaacaatg attaatatca gacgcaaatc ctgcatcatt atattctctg ttgttctaac
    31141 accttgccac cacggcaaac atttactcac taagagtatt tgccgattac ctcaagtgca
    31201 agtgcactat taactttcac agcacaaaga tagatgaaat cgtgcttttt gctgtttttt
    31261 cgaacatatc ctaactgtcc attgcgcaat tacccggtaa aatacgcaga attttcctgg
    31321 gattggtcaa aaggagctca tcctgattat ggtttcatca ttcacatctg ccccacgcag
    31381 cggtttttac tattttgcgc aaggctggaa gctcgtctcg caacctggga ttcggcgttt
    31441 cgttatttta ccgctgctgg tcaatatttt gttgatgggg ggcgcattct ggtggctctt
    31501 tacacagctc aatgtctgga tcccgactct catgagttac gttccggact ggctacaatg
    31561 gctgagttat ctgttgtggc ctctggctgt catctctgtg ctgttagtgt ttggctattt
    31621 cttctccacg attgctaact ggattgccgc tccgtttaac ggtttattgg ctgaacaact
    31681 ggaagcacga ttgactggcg ctacaccgcc agataccggg attttcggta tcatgaaaga
    31741 tgtgccgcga atcatgaaac gcgaatggca aaaatttgcc tggtatctgc cgcgcgcaat
    31801 tgtattacta attctttact tcatccctgg tattgggcaa accgtcgcgc cggtactgtg
    31861 gttcctgttt agcgcctgga tgttagccat ccagtattgc gattacccct tcgataacca
    31921 caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca
    31981 gtttggtgct ttaaccagcc tgtttacgat gatcccgctg cttaatctgt tcatcatgcc
    32041 cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat
    32101 gtggcggtaa caatctaccg gttattttgt aaaccgtttg tgtgaaacag gggtggctta
    32161 tgccgcccct tattccatct tgcatgtcat tatttccctt ctgtatatag atatgctaaa
    32221 tccttacttc cgcatattct ctgagcgggt atgctacctg ttgtatccca atttcataca
    32281 gttaaggaca ggccatgagt aagatttttg aagataactc gctgactatc ggtcacacgc
    32341 cgctggttcg cctgaatcgc atcggtaacg gacgcattct ggcgaaggtg gaatctcgta
    32401 accccagctt cagcgttaag tgccgtatcg gtgccaacat gatttgggat gccgaaaagc
    32461 gcggcgtgct gaaaccaggc gttgaactgg ttgaaccgac cagcggtaat accgggattg
    32521 cactggccta tgtagctgcc gctcgcggtt acaaactcac cctgaccatg ccagaaacca
    32581 tgagtattga acgccgcaag ctgctgaaag cgttaggtgc aaacctggtg ctgacggaag
    32641 gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga aattgtcgcc agcaatccag
    32701 agaaatacct gctgctgcaa caattcagca atccggcaaa ccctgaaatt cacgaaaaaa
    32761 ccaccggtcc ggagatctgg gaagataccg acggtcaggt tgatgtattt attgctggcg
    32821 ttgggactgg cggtacgctg actggcgtca gccgctacat taaaggcacc aaaggcaaga
    32881 ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc agttatcgcc caggcgctgg
    32941 caggtgaaga gattaaacct ggcccgcata aaattcaggg tattggcgct ggttttatcc
    33001 cggctaacct cgatctcaag ctggtcgata aagtcattgg catcaccaat gaagaagcca
    33061 tttctaccgc ccgtcgtcta atggaagaag aaggtattct tgcaggtatc tcttctggag
    33121 cagctgttgc cgcggcgttg aaactacaag aagatgaaag ctttaccaat aagaatattg
    33181 tggttattct accatcatcg ggtgagcgtt atttaagcac cgcattgttt gccgatctct
    33241 tcactgagaa agaattgcaa cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct
    33301 ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc ctcctggcat tgattcagcc
    33361 tgtcggaact ggtatttaac cagactaatt attttgatgc gcgaaattaa tcgttacagg
    33421 aaaagccaaa gctgaatcga ttttatgatt tggttcaatt cttcctttaa cggcataatg
    33481 tttaatgacg tacgaaacgt cagcggtcaa cacccgccag caatggactg tattgcgctc
    33541 ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac aggctaaagt cgaaccgcca
    33601 ggctagactt tagttccaca acactaaacc tataagttgg ggaaatacaa tgttccagca
    33661 agaagttacc attaccgctc cgaacggtct gcacacccgc cctgctgccc agtttgtaaa
    33721 agaagctaag ggcttcactt ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc
    33781 gaaaagcctg tttaaactgc agactctggg cctgactcaa ggtaccgttg tgactatctc
    33841 cgcagaaggc gaagacgagc agaaagcggt tgaacatctg gttaaactga tggcggaact
    33901 cgagtaattt cccgggttct tttaaaaatc agtcacaagt aaggtagggt tatgatttca
    33961 ggcattttag catccccggg tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa
    34021 attgtcattg accggaaaaa aatttctgcc gaccaggttg atcaggaagt tgaacgtttt
    34081 ctgagcggtc gtgccaaggc atcagcccag ctggaaacga tcaaaacgaa agctggtgaa
    34141 acgttcggtg aagaaaaaga agccatcttt gaagggcata ttatgctgct cgaagatgag
    34201 gagctggagc aggaaatcat agccctgatt aaagataaac acatgacagc tgacgcagct
    34261 gctcatgaag ttatcgaagg tcaggcttct gccctggaag agctggatga tgaatacctg
    34321 aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc
    34381 ctgaagatta tcgacctgag cgccattcag gatgaagtca ttctggttgc cgctgacctg
    34441 acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc tgggtttcat caccgacgcg
    34501 ggtggccgta cttcccacac ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg
    34561 ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact atctgattct ggatgccgta
    34621 aataatcagg tttacgtcaa tccaaccaac gaagttattg ataaaatgcg cgctgttcag
    34681 gagcaagtgg cttctgaaaa agcagagctt gctaaactga aagatctgcc agctattacg
    34741 ctggacggtc accaggtaga agtatgcgct aacattggta cggttcgtga cgttgaaggt
    34801 gcagagcgta acggcgctga aggcgttggt ctgtatcgta ctgagttcct gttcatggac
    34861 cgcgacgcgc tgcccactga agaagaacag tttgctgctt acaaagcagt ggctgaagcg
    34921 tgtggctcgc aggcggttat cgttcgtacc atggacatcg gcggcgacaa agagctgcca
    34981 tacatgaact tcccgaaaga agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg
    35041 atggatcgta aagagatcct gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc
    35101 ggtaaattgc gcattatgtt cccgatgatc atctctgttg aagaagtgcg tgcactgcgc
    35161 aaagagatcg aaatctacaa acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca
    35221 attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa caattgcacg tcatttagcc
    35281 aaagaagttg atttctttag tatcggcacc aatgatttaa cgcagtacac tctggcagtt
    35341 gaccgtggta atgatatgat ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac
    35401 ttgatcaagc aagttattga tgcttctcat gctgaaggca aatggactgg catgtgtggt
    35461 gagcttgctg gcgatgaacg tgctacactt ctgttgctgg ggatgggtct ggacgaattc
    35521 tctatgagcg ccatttctat cccgcgcatt aagaagatta tccgtaacac gaacttcgaa
    35581 gatgcgaagg tgttagcaga gcaggctctt gctcaaccga caacggacga gttaatgacg
    35641 ctggttaaca agttcattga agaaaaaaca atctgctaat ccacgagatg cggcccaatt
    35701 tactgcttag gagaagatca tgggtttgtt cgataaactg aaatctctgg tttccgacga
    35761 caagaaggat accggaacta ttgagatcat tgctccgctc tctggcgaga tcgtcaatat
    35821 cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa
    35881 accaacgggt aacaaaatgg tcgcgcctgt agacggtacc attggtaaaa tctttgaaac
    35941 caatcacgcg ttctctatcg aatctgatag cggcgttgaa ctgttcgtcc acttcggtat
    36001 cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt
    36061 gaaagttggc gatactgtca ttgaatttga tctgccgctg ctggaagaga aagccaagtc
    36121 taccctgact ccggttgtta tctccaacat ggacgaaatc aaagaactga tcaaactgtc
    36181 cggtagcgta accgtgggtg aaaccccggt tatccgcatc aagaagtaat tcttgccgca
    36241 gtgaaaaatg gcgccca
//
LOCUS       DAZPJP010000022        36199 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPJP010000022 DAZPJP010000000
VERSION     DAZPJP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.022"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            271..606
                     /locus_tag="ACHU9J_000985"
     CDS             271..606
                     /locus_tag="ACHU9J_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1904592.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            764..1207
                     /gene="yjdN"
                     /locus_tag="ACHU9J_000986"
                     /pseudo
     CDS             764..1207
                     /gene="yjdN"
                     /locus_tag="ACHU9J_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1340..2128
                     /gene="phnC"
                     /locus_tag="ACHU9J_000987"
     CDS             1340..2128
                     /gene="phnC"
                     /locus_tag="ACHU9J_000987"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1904593.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2153..3169
                     /gene="phnD"
                     /locus_tag="ACHU9J_000988"
     CDS             2153..3169
                     /gene="phnD"
                     /locus_tag="ACHU9J_000988"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1904594.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3295..4074
                     /gene="phnE"
                     /locus_tag="ACHU9J_000989"
     CDS             3295..4074
                     /gene="phnE"
                     /locus_tag="ACHU9J_000989"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1904595.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLCKHFI"
     gene            4095..4820
                     /gene="phnF"
                     /locus_tag="ACHU9J_000990"
     CDS             4095..4820
                     /gene="phnF"
                     /locus_tag="ACHU9J_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1904596.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4821..5273
                     /gene="phnG"
                     /locus_tag="ACHU9J_000991"
     CDS             4821..5273
                     /gene="phnG"
                     /locus_tag="ACHU9J_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR1904597.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5270..5854
                     /gene="phnH"
                     /locus_tag="ACHU9J_000992"
     CDS             5270..5854
                     /gene="phnH"
                     /locus_tag="ACHU9J_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1904598.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5854..6918
                     /gene="phnI"
                     /locus_tag="ACHU9J_000993"
     CDS             5854..6918
                     /gene="phnI"
                     /locus_tag="ACHU9J_000993"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1904599.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6911..7756
                     /gene="phnJ"
                     /locus_tag="ACHU9J_000994"
     CDS             6911..7756
                     /gene="phnJ"
                     /locus_tag="ACHU9J_000994"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1904600.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7753..8511
                     /gene="phnK"
                     /locus_tag="ACHU9J_000995"
     CDS             7753..8511
                     /gene="phnK"
                     /locus_tag="ACHU9J_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1904601.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8622..9302
                     /gene="phnL"
                     /locus_tag="ACHU9J_000996"
     CDS             8622..9302
                     /gene="phnL"
                     /locus_tag="ACHU9J_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1904602.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9299..10435
                     /gene="phnM"
                     /locus_tag="ACHU9J_000997"
     CDS             9299..10435
                     /gene="phnM"
                     /locus_tag="ACHU9J_000997"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR1904603.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10435..10992
                     /gene="phnN"
                     /locus_tag="ACHU9J_000998"
     CDS             10435..10992
                     /gene="phnN"
                     /locus_tag="ACHU9J_000998"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1904604.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10979..11413
                     /gene="phnO"
                     /locus_tag="ACHU9J_000999"
     CDS             10979..11413
                     /gene="phnO"
                     /locus_tag="ACHU9J_000999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1904605.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11415..12173
                     /gene="phnP"
                     /locus_tag="ACHU9J_001000"
     CDS             11415..12173
                     /gene="phnP"
                     /locus_tag="ACHU9J_001000"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1904606.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12320..12649)
                     /gene="yjdP"
                     /locus_tag="ACHU9J_001001"
     CDS             complement(12320..12649)
                     /gene="yjdP"
                     /locus_tag="ACHU9J_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1904607.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12718..13035)
                     /locus_tag="ACHU9J_001002"
     CDS             complement(12718..13035)
                     /locus_tag="ACHU9J_001002"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1904608.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13288..15273
                     /gene="yjcS"
                     /locus_tag="ACHU9J_001003"
     CDS             13288..15273
                     /gene="yjcS"
                     /locus_tag="ACHU9J_001003"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1904609.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            15482..15757
                     /locus_tag="ACHU9J_001004"
     CDS             15482..15757
                     /locus_tag="ACHU9J_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1904610.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            15776..16807
                     /gene="mdtN"
                     /locus_tag="ACHU9J_001005"
     CDS             15776..16807
                     /gene="mdtN"
                     /locus_tag="ACHU9J_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1904611.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            16807..18858
                     /gene="mdtO"
                     /locus_tag="ACHU9J_001006"
     CDS             16807..18858
                     /gene="mdtO"
                     /locus_tag="ACHU9J_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1904612.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            18855..20321
                     /gene="mdtP"
                     /locus_tag="ACHU9J_001007"
                     /pseudo
     CDS             18855..20321
                     /gene="mdtP"
                     /locus_tag="ACHU9J_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            20519..22666
                     /gene="fdhF"
                     /locus_tag="ACHU9J_001008"
     CDS             20519..22666
                     /gene="fdhF"
                     /locus_tag="ACHU9J_001008"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20936..20938,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1904613.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            22760..23449
                     /gene="yjcO"
                     /locus_tag="ACHU9J_001009"
     CDS             22760..23449
                     /gene="yjcO"
                     /locus_tag="ACHU9J_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1904614.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(23528..24841)
                     /gene="gltP"
                     /locus_tag="ACHU9J_001010"
     CDS             complement(23528..24841)
                     /gene="gltP"
                     /locus_tag="ACHU9J_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1904615.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(25183..25779)
                     /gene="nrfG"
                     /locus_tag="ACHU9J_001011"
     CDS             complement(25183..25779)
                     /gene="nrfG"
                     /locus_tag="ACHU9J_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1904616.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(25776..26159)
                     /gene="nrfF"
                     /locus_tag="ACHU9J_001012"
     CDS             complement(25776..26159)
                     /gene="nrfF"
                     /locus_tag="ACHU9J_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1904617.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(26152..27870)
                     /locus_tag="ACHU9J_001013"
     CDS             complement(26152..27870)
                     /locus_tag="ACHU9J_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1904618.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(27890..28846)
                     /gene="nrfD"
                     /locus_tag="ACHU9J_001014"
     CDS             complement(27890..28846)
                     /gene="nrfD"
                     /locus_tag="ACHU9J_001014"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1904619.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(28843..29514)
                     /gene="nrfC"
                     /locus_tag="ACHU9J_001015"
     CDS             complement(28843..29514)
                     /gene="nrfC"
                     /locus_tag="ACHU9J_001015"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1904620.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(29511..30077)
                     /gene="nrfB"
                     /locus_tag="ACHU9J_001016"
     CDS             complement(29511..30077)
                     /gene="nrfB"
                     /locus_tag="ACHU9J_001016"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1904621.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(30122..31558)
                     /gene="nrfA"
                     /locus_tag="ACHU9J_001017"
     CDS             complement(30122..31558)
                     /gene="nrfA"
                     /locus_tag="ACHU9J_001017"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1904622.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            31952..33910
                     /gene="acs"
                     /locus_tag="ACHU9J_001018"
     CDS             31952..33910
                     /gene="acs"
                     /locus_tag="ACHU9J_001018"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR1904623.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            34110..34423
                     /locus_tag="ACHU9J_001019"
                     /pseudo
     CDS             34110..34423
                     /locus_tag="ACHU9J_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            34420..36069
                     /gene="actP"
                     /locus_tag="ACHU9J_001020"
     CDS             34420..36069
                     /gene="actP"
                     /locus_tag="ACHU9J_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1904624.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         8431 a        10203 c         9736 g         7829 t
ORIGIN      
        1 taacgtcttc tgatgcgttt aaaacgtcag aaagataaaa atatcatgtg aataaaaaaa
       61 agaacaagta gagcattaac attatcttaa ataataaata gaggcaaaaa gattattttc
      121 tttttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt acccccgaca
      181 aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt ttttggcgct
      241 tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc aaaatgcaac
      301 tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc ctacgaatgg
      361 aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc taacggcaat
      421 ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa aggtagctct
      481 tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg cgaccataac
      541 atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt tgtgaaaaag
      601 aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc cggcaacgat
      661 gccgactgcc tgatgcgacg cttgcgcgtc ttatcaggca gcctctcatc tgtataaatt
      721 tcgaactaca cttaattggc ttctcttaac tgaggtcacc atcatgccgt taagtcccta
      781 cctctctttt gccggtaact gtgccgacgc aattgcttat tatcaacgta cgttgggcgc
      841 tgaactgctc tataaaatca gcttcggcga aatgccaaaa tcagcgcagg acagcgccga
      901 gaactgccct tccggaatgc aatttcccga taccgccatc gcccacgcca atgtgcgcat
      961 tgccggaagc gacatcatga tgagcgatgc catgccgtca ggaaaagcca gctactccgg
     1021 ctttacgctg gtgctcgatt cgcaacaggt cgaagaagga aaacgctggt ttgacaatct
     1081 tgccgctaac ggaaaaatcg aaatggcctg gcaggaaact ttctaggcgc atggctttgg
     1141 caaagtcacc gataaatttg gcgtaccgtg gatgattaat gtcgtcaaac aacaaccaac
     1201 gcaataaccc gccgggaggc ccgccctccc gcactgtcat cgaattcccg ttaactcttc
     1261 atctgttagt cacttttaat taaccaaatc gtcacaataa tccgccacga tggagccact
     1321 tttttaggga ggctgcatca tgcaaacgat tatccgtgtc gagaagctcg ccaaaacctt
     1381 caatcagcat caggcgctgc atgcggttga tctgaacatt catcacggtg aaatggtggc
     1441 tctgcttggg ccgtcgggtt ccggcaaatc caccctttta cgtcacttaa gcggtttgat
     1501 taccggcgat aaatccgccg gcagccatat cgagctgctg ggccgcacag tccagcgcga
     1561 aggccgtctg gcgcgcgata tccgcaaaag ccgcgccaac accggctaca tcttccaaca
     1621 attcaacctg gtgaaccgcc tgagcgtact ggagaacgtg ctgattggcg cactcggcag
     1681 cacgccgttc tggcgcacct gttttagctg gtttacccgc gagcagaaac aacgcgcgtt
     1741 acaggcgctg acccgcgttg gcatggtgca ttttgcccat caacgcgttt ccaccctctc
     1801 cggcggacag cagcagcgtg tggcgattgc ccgcgcgctg atgcagcagg cgaaggtgat
     1861 tctggccgat gaacccatcg cctcgctgga cccggaatcc gccggcatcg tgatggacac
     1921 cctgcgcgac atcaatcaga acgacggcat caccgtggtc gtcacgctgc atcaggtgga
     1981 ttacgccctg cgctactgcg aacgcatcgt cgccctgcgc caggggcacg ttttctacga
     2041 cggcagcagc caacagtttg ataacgaacg ttttgaccat ctctaccgca gcattaatcg
     2101 catcgaagag aacgcgaaag ctgcctgaca tccccatcat tgaggaaaac gaatgaacgc
     2161 taagataatt gcctcgctgg ccttcaccag catgttcagc ctcagcaccc tgttaagccc
     2221 ggcacacgcc gaagagcagg aaaaggcgct gaatttcggc attatttcaa cggaatcaca
     2281 gcaaaacctg aaaccgcaat ggacgccatt cttacaggat atggagaaga agctgggcgt
     2341 gaaggtgaac gccttctttg ccccagacta cgcaggcatt atccagggaa tgcgcttcaa
     2401 taaagtggat atcgcctggt acggcaacct gtcggcaatg gaagcggtgg atcgcgccaa
     2461 cggccaggtc ttcgcccaga cggtcgcggc ggatggatcg ccaggttact ggagcgtgtt
     2521 gatcgtcaac aaagatagtc cgatcaacaa cctgaacgat ctgctggcga agcggaaaga
     2581 tctcaccttc ggcaatggcg atcctaactc cacctctggc ttcctcgtcc ccggttacta
     2641 cgtctttgcc aaaaacaata tctccgccag cgactttaag cgcaccgtca acgccgggca
     2701 tgaaaccaac gcgctggccg tcgccaacaa gcaggtggat gttgccacca acaacaccga
     2761 aaacctcgac aagctgaaaa cctccgcgcc agagaagctg aaagaactga aggtgatctg
     2821 gaagtcgccg ctgatcccag gcgatccgat cgtctggcgc aagaatctct ccgaaaccac
     2881 caaagacaag atctacgact tctttatgaa ctacggaaaa acgccggaag agaaagcggt
     2941 gctggaacgc ctgggctggg caccgtttcg cgcctccagc gacctgcaac tggtgccgat
     3001 tcgccagctc gcactgttta aagagattca gggcgtaaaa agcaataacg gactgaatga
     3061 gcaggacaag ctggcgaaaa ccaccgagat tcaggcgcag ctggatgacc tggaccgcct
     3121 gaacaacgcg ttaagcgcga tgagttcggt gagtaaagcg gtgcagtaat gtgctcgatc
     3181 ggtcccctct cccttgaggg agagggttag ggtgaggggg aaatgcgcac gctgatgccc
     3241 tcaccccggc cctctcccac agggagaggg agaaaacatt aaacggagcc aaacatgcaa
     3301 accatcacca tcgccccacc caagcgcagc tggttctcgc ttctgagctg ggccattgtt
     3361 ctcgccgtgt tggtcgtctc gtggcagggc gcggaaatgg ccccgcttac gctgatcaaa
     3421 gacggcggca acatggcgac cttcgccgcc gacttcttcc cgcccgattt cagccagtgg
     3481 caggattacc tcaccgaaat ggccgtcacg ctgcaaatcg ccgtctgggg caccgcgctg
     3541 gcagtggttc tctccatccc cttcggcctg atgagcgccg aaaacctggt gccgtggtgg
     3601 gtttaccagc ccgttcgccg cctgatggac gcctgccgcg ccattaacga aatggtcttc
     3661 gccatgctgt tcgtggtcgc cgtcggtctc ggaccgttcg ctggcgtgct ggcgctattt
     3721 attcacacca ccggcgtgct ctcgaaactg ctttccgaag cggtggaagc gattgaaccc
     3781 ggcccggtgg aaggcattcg cgccaccggc gccaacaagc tggaagagat cctttacggc
     3841 gtgctgccac aggtaatgcc gctgctgatc tcctactccc tctatcgctt cgaatccaac
     3901 gtccgctcgg cgaccgtcgt cggcatggtc ggtgcaggcg ggatcggcgt caccctgtgg
     3961 gaagcgattc gcggtttcca gttccaacaa acctgcgccc tgatggtgct tatcatcgtc
     4021 acggtcagcc tgctggattt cctctctcaa cggttgtgta agcactttat ctgataagtg
     4081 aggcattgat atctatgcac ttgtctacac atccgaccag ctacccaaca cgctatcagg
     4141 agatagccgc aaaacttgag caggagcttc gtcaacacta tcgctgcggc gactatcttc
     4201 ccgccgagca gcaactggcg gcgcgctttg aggtaaaccg ccacaccctg cgccgcgcca
     4261 tcgaccaact ggtggaaaaa ggctgggtac agcgccgtca gggcatcggc gtgctggtgc
     4321 tgatgcgccc gttcgattac ccgctcaacg cccaggcgcg ttttagccag aacctgctgg
     4381 accagggcag ccatcccacc agcgaaaaac tgctgtcggt attgcgcccc gcgtccggtc
     4441 acgttgctga cgcgctgggg attaccgagg gtgagaacgt tatccatttg cgcaccctgc
     4501 gtcgcgtcaa cggcgtcgcg ctctgtttaa tcgaccacta ctttgcggac ctcaccctct
     4561 ggccgacact gcaacgtttc gacagcggtt cgctgcacga ttttctgcgc gagcaaaccg
     4621 gaattgcgct gcgccgcagc cagacgcgga tcagcgcccg ccgcgcccag gccaaagagt
     4681 gccagcgcct tgaaatcccg aatatgtcgc cgctgctgtg cgtgcgcacc cttaaccacc
     4741 gtgacggtga aagcagcccg gcggagtact ccgtcagcct gacgcgcgcc gacatgattg
     4801 aattcactat ggagcactga atgcacgcag ataccgcgac ccgccagcac tggatgtccg
     4861 tactggcgca cagccaaccg gctgaactgg cagcccgcct gaacgcgctg aacatcaccg
     4921 ccgactatga agtgatccgc actgccgaaa ctggcctggt gcagattcag gcgcgaatgg
     4981 gcggtaccgg cgaacgcttt tttgccggcg acgccacgct gacccgtgcc gccgtacgcc
     5041 tgactgacgg caccctcggc tacagctggg tgctggggcg tgataaacag cacgccgaac
     5101 gctgcgcgct gattgatgcg ctgatgcagc aatctcgcca ctttcaaaac ttatcagaaa
     5161 cccttattgc cccgctggac gctgaccgta tggcacgcat tgccgcacgc caggccgaag
     5221 tgaacgccag ccgggtcgac ttctttacga tggttcgcgg agacaacgca tgaccctgga
     5281 aaccgctttt atgcttcccg tgcaggatgc ccagcacagt tttcgtcgcc tgttaaaggc
     5341 catgagcgag ccgggcgtga ttgtcgccct gtatcagctc aaacgcggct ggcagccgct
     5401 gaatatcgcc accaccagcg tgctgctgac gctggccgat aacgacacgc cggtgtggct
     5461 tgccacgcca ttaagtaacg atatcgtcaa tcagagcctg cgttttcata ccaacgcgcc
     5521 gctggtcagc cagccggaac aggcgacctt cgcggtgacg gatgaggcga tttccagcga
     5581 acagctcaac gccctttcca ccgacaccgc cgttgcgccg gaagcgggcg cgacgctgat
     5641 tttacaggtc gccagcctga gcggcggacg catgttgcgt ctcaccggcg cgggcattgc
     5701 cgaagaacga atgatcgctc cgcagctgcc agagtgcatt ctgcacgaac tcaccgagcg
     5761 cccgcacccg ttcccgctcg gcatcgacct aatcctgacc tgcggcgagc gcctgctggc
     5821 tatcccgcga accactcatg tggaggtgtg ctgatgtacg ttgccgtgaa agggggcgaa
     5881 aaggcgatcg acgccgccca cgccctgcaa gagagccgac gccggggcga taccgatttg
     5941 cccgaactga gcgtcgccca gattgaacag cagcttaacc tcgcggtgga tcgcgtgatg
     6001 acagaaggcg gcattgccga ccgcgaactg gcggcgctgg cgctgaaaca ggccagcggc
     6061 gataacgtcg aagcgatttt cctgctacgc gcctaccgca ccacgctgac gaaactggcg
     6121 gtaagcgagc cgctcgacac cacggagatg cgtctcgaac ggcgtatttc agcggtttat
     6181 aaagatattc ccggcggcca gctgcttggc ccaacctacg actacaccca tcgcctgctc
     6241 gatttcactc tgctggcaaa cggcgaagcg ccgacgctga ccaccgccga cagcgaacaa
     6301 cagccgtcgc cgcacgtttt cagcctgctg gcgcgtcagg ggctggcgaa gtttgaagag
     6361 gatagcggag cacagccgga tgacatcacc cgcacgccgc cggtttaccc ctgttcacgt
     6421 tcttcccgtt tgcagcagtt aatgcgcggc gacgaaggct atttgctggc gctggcttac
     6481 tccacccagc gcggttacgg gcgcaaccac ccgttcgcgg gcgagatccg cagtggttac
     6541 atcgacgtgt cgattgtgcc ggaagagctg ggatttgcgg taaacgtcgg cgaactgctg
     6601 atgaccgagt gtgaaatggt caacggtttt atcgacccgc cggatgagcc gccgcacttc
     6661 acgcgcggct acgggctggt attcggcatg agcgagcgca aagcgatggc aatggcgctg
     6721 gttgaccgtg cgttgcaagc cccggaatac ggcgagcacg cgacaggccc ggcgcaggat
     6781 gaagagttcg tgctggcgca tgccgacaac gtcgaagccg caggctttgt ctcgcacctc
     6841 aaactcccgc actacgtcga tttccaggcc gaactggagc tactcaaacg tctgcaacag
     6901 gagcagaacc atggctaatc tgagcggcta caactttgcc tacctcgacg agcagaccaa
     6961 acgcatgatc cgccgcgcca tcttaaaagc ggtggcgatc cccggttatc aggtaccgtt
     7021 tggcgggcgc gagatgccga tgccgtacgg ctggggaact ggtggtatcc agctcaccgc
     7081 cagcgtgatt ggcgaaagcg acgtgttgaa ggtgattgac cagggtgcgg atgacaccac
     7141 caacgccgtg tcgattcgca acttctttaa gcgcgtgacc ggggtaaaca ccactgaatg
     7201 tacggacgat gcgacgctta tccagacgcg tcaccgcatc cccgaaacgc cgctgaccga
     7261 agatcagatc attatcttcc aggtgccaat cccggaaccg ctgcgcttta tcgagccgcg
     7321 cgaaacggag acccgcacta tgcacgcgct ggaggagtac ggcgtgatgc aggtgaaact
     7381 gtatgaagat atcgcccgct tcggtcatat cgccaccacc tatgcttatc cggtgaaggt
     7441 gaacgggcgc tacgtgatgg acccgtcgcc gatcccgaaa ttcgataacc cgaaaatgga
     7501 catgatgccc gccctgcagc tgttcggcgc agggcgcgag aagcgcatct atgcggtgcc
     7561 gccgtttacc cgcgtggaaa gtctcgattt cgacgatcac ccgttcaccg ttcagcagtg
     7621 ggatgagcca tgcgccatct gcggatcgac ccacagctat cttgatgaag tggtgctgga
     7681 tgacgccgga aaccgcatgt ttgtctgctc cgataccgat tattgccgcc aacagagcga
     7741 ggcgaaaagc caatgaatca accgttactt tcggtcaata acctgaccca cctttacgcg
     7801 ccgggcaaag gctttagcga tgtctctttt gatttatggc cgggggaagt gctgggcatt
     7861 gtcggggaat ccggctccgg gaagaccacg ctgctgaagt cgatctccgc gcgtctgacg
     7921 ccgcagcagg gggagattca ctatgaaaac cgttccctct atgcaatgag cgaggccgac
     7981 cgccgtcgcc tgctgcgcac cgaatggggc gtggtgcatc agcatccgct cgacggcctg
     8041 cgccgccagg tgtcggcagg cggcaatatc ggcgagcggc tgatggcgac cggggcgcga
     8101 cattacggcg atattcgcgc taccgcgcag aagtggctgg aagaggtgga gattcccgcc
     8161 aaccgtatcg acgacctgcc gaccaccttt tccggcggta tgcagcagcg tttgcagatt
     8221 gcccgcaacc tggtgacgca tccgaagctg gtgtttatgg atgaaccgac cggcgggctg
     8281 gatgtgtcgg tgcaggcccg cctgctcgac ctgctgcgcg gactggtggt ggagctgaac
     8341 ctcgcggtag tgattgtcac ccacgattta ggcgtcgccc gcctgctggc ggaccgtttg
     8401 ctggtgatga agcaggggca agtggtggag agtgggttaa ccgaccgcgt gctcgacgac
     8461 ccgcatcatc cgtatacaca gctgctggtg tcatcggttt tgcagaattg agccggtgcc
     8521 ggatgcggcg taaacgcctt atccggccta caaatgcgct ccccgtaggt cggataagac
     8581 gcgtcagcgt cgcatccgac acccgaacca cgaggcgaaa aatgattaac gtacaaaacg
     8641 tcagtaaaac cttcatcctg caccagcaaa acggcgtgcg cctgcccgtc ctcaaccgcg
     8701 cctcgctcat cgtcaacgcg ggcgaatgcg tggtgctcca cggccattcc ggcagcggaa
     8761 aatcaaccct gctacgctcg ctgtacgcca actatctgcc cgacgaaggg caaatccaga
     8821 tcaaacacgg tgacgaatgg gtcgatctgg tcaccgcgcc agcgcgcaaa gtggtggaga
     8881 tccgcaaaac caccgttggc tgggtgagcc agtttctgcg cgtcatcccg cgtatttcgg
     8941 cgctggaagt ggtgatgcag ccgctactcg ataccggcgt accgcgtgaa gcctgcgccg
     9001 ctaaagccgc gcgtctgctc acccgcctga acgtgccgga acgcctgtgg catctggcgc
     9061 catcgacctt ctccggcggc gaacagcagc gcgtcaacat cgcccgcggc tttatcgtcg
     9121 actaccccat tctgctgctc gacgaaccta ccgcctcgct ggacgccaaa aacagcgccg
     9181 ccgtagtcga gctgattcac gaagccaaag cccgtggcgc ggccatcgtc ggcatcttcc
     9241 acgacgaagc cgtacgtaat gacgttgccg accgcctgca cccaatggaa gcctcttcat
     9301 gattatcaat aacgtcaaat tggtgctgga aaacgaagtg gtacacggtt cactggaggt
     9361 gcaggacggc gaaatccgcg cctttgccga aagccagagc cgcctgccgg aggcgatgga
     9421 cggcgaaggc ggctggctgc tgccggggct gattgagctg cataccgata atctggataa
     9481 attcttcacc ccgcgcccga aagttgactg gcctgcccac tcggcgatga gcagccacga
     9541 cgcgctgatg gtggcgagcg gcatcaccac cgtactggat gccgtggcaa ttggcgacgt
     9601 gcgcgacggc ggcgatcggc tggtgaatct ggagaagatg atcaacgcca tcgaagagac
     9661 gcagaaacgc ggcgtcaacc gcgccgagca ccgtctgcat ctgcgctgcg aactgccgca
     9721 tcacaccacg ctgccgctgt ttgaaaaact ggtgcagcgc gagccggtga cgctggtgtc
     9781 gctgatggac cactcgccgg gccagcgcca gttcgccaat cgcgagaagt atcgcgaata
     9841 ttatcagggc aaatactccc tcactgatgc gcagatgcag cagtacgaag aagagcaact
     9901 ggcgctcgcc gcacgctggt cgcagccgaa tcgcgaatcc atcgccgccc tgtgccgcgc
     9961 gcgaaaaatt gcgcttgcca gccacgatga cgccacccac gcccacgttg ctgaatctca
    10021 ccagcttggc agcgtgatcg ccgaatttcc caccacgttc gaagcggcgg aagcctcgcg
    10081 caagcatggc atgaacgtgc tgatgggcgc gccgaatatt gtgcgcggcg gctcgcactc
    10141 cggcaacgtg gcagccagtg aactggcgca gcttggcctg ctggatatcc tctcttccga
    10201 ctactacccc gccagcctgc tcgatgcggc atttcgcgtc gccgatgacg agagcaaccg
    10261 ctttacgctg ccgcaggcgg tgaagctggt gactaaaaat ccagcgcagg cgcttaatct
    10321 ccaggatcgc ggggtgattg gcgagggcaa acgcgcggac ttggtgctgg cgcatcgcaa
    10381 gggcaatcac attcatatcg accacgtctg gcgtcagggt aaaagggtgt tctgatgatg
    10441 ggaaaactga tttggttaat ggggccgtcc ggctccggga aagatagcct gctggcggaa
    10501 ctccgcctgc gggaacaaac tcagctactg gtggcgcatc gctacatcac gcgcgacgcc
    10561 agcgccggaa gcgaaaacca tatcgctctg agcgagcagg agttttttac ccgcgcgggg
    10621 cagaacctac tggccttaag ctggcacgct aacggtctgt attatggcgt cggcgtcgag
    10681 atcgacctct ggctgcacgc cggattcgac gtgctggtca acggctcacg cgcccatctg
    10741 ccgcaggcgc gggcgcgcta tcaatcggcg ctgctgcccg tctgtttaca ggtttcgccg
    10801 gagatcctcc gccagcgcct ggaaaaccgt ggccgtgaaa acgccagtga aattaacgcc
    10861 cgcctggcgc gcgccgcccg ctatactcca caggattgcc atacgctcaa caatgacggc
    10921 agcctgcgcc agtcggtcga cacgctgctg acgctgattc atcagaagga gaaacaccat
    10981 gcctgcttgt gagcttcgcc cggccacgca gtatgatacc gacgcggttt acgcgctgat
    11041 ttgtgagcta aaacaggcgg agtttgacca ccacgcattt cgcgtgggct ttagcgccaa
    11101 tctgcgcgac ccgaacatgc gctatcatct ggcgctgctg gatggcgagg ttgtcggcat
    11161 gatcggcctg catttgcagt ttcatctgca tcatgtcaac tggatcggcg aaattcagga
    11221 actggtagtg atgccgcagg cgcgcggcct gaacgtcggt agcaagttgc tggcgtgggc
    11281 agaagaagaa gcccgccagg ccggggccga aatgaccgag ctttcgacca acgtgaagcg
    11341 ccacgacgcg caccgtttct atctgcgaga aggctacgag cagagtcact tccgcttcac
    11401 caaggcgctg taacatgagt ctgaccctca cgctcaccgg caccggcggc gcacagggcg
    11461 ttccggcatg gggctgcgag tgcgcggcct gcgaccgagc gcggcgctcg ccgcagtatc
    11521 gccgccaacc gtgcagcggc gtagtgaagt ttaacgaggc gatcaccctg atcgacgccg
    11581 ggctgcacga tctcaccgat cgctggtctc ccggatcgtt ccagcagttt ttgctgacgc
    11641 attatcatat ggatcacgtc caggggctgt ttccgctgcg ctggggcgtg ggcgatccga
    11701 tcccgatcta cggcccgccg gatgaacagg gctgcgacga tctgtttaaa catcccgggc
    11761 tgcttgattt cagccacacg gtggaaccgt ttgtggtgtt tgatttgcag gggttgcagg
    11821 tcacgccgct gccgcttaac cactcaaaac tcactttcgg ctatttactg gaaacggcgc
    11881 acagccgggt ggcgtggctg tctgacaccg ctggcttgcc ggaaaaaacg ctgaaatttt
    11941 tacgcaataa tcagccgcag gtaatggtga tggattgcag tcacccgccg cgcgcggatg
    12001 caccgcgtaa tcactgtgat ttaaataccg tgcttgcgct gaatcaggtt atccgctcgc
    12061 cacgggtgat tctgacccat atcagccacc agtttgatgc gtggctgatg gaaaacgcac
    12121 taccgtcagg gtttgaggtg gggtttgatg ggatggagat tggggtggcg tgatgagagg
    12181 gaatgtgcgc gctggccccc tcaccctaac cctctcccca gaggggcgag gggaccgatt
    12241 gtgctcgata ttgaatattg cgctcgtttt ctccctctcc ccattggggt gaggggcgat
    12301 gcctgctcca tacccaacct catcgcccat actcatcttc cattctccgc tcttcatcct
    12361 ccagttgccg acgctcctga tcaagctggc gctggcgatc gtccagctgc ctgcggcgat
    12421 cttcaaactg gcggcggcgg tcgtcatatt gtctgcgccg atcgtcgctc acttcacgct
    12481 gccagccgtc gtcgcgcgaa tcttcatagt ctcgcccacg gtcagggtta taagcgtcat
    12541 taatcgcctg ctgaatatta ccaatagtat cgtcgataat atcagcctgg gccggaacgg
    12601 tggacagcgt gagcagggtg aataaaagaa atagcggaaa gcgtttcatt agccaacctc
    12661 aaaaagaaac tctatccaca ttagtcatta ctcatccatg caagtagtgg atgaatctca
    12721 atttctccgc tgctctattg ccgtaatcgc ctccacgcgt tgttgatgac gaccgccttc
    12781 gtactgtgcg cccagccacg catccacaat catttttgcc agttcgaggc caaccactcg
    12841 tgaaccaaaa gccagtgcaa cttcactggc ataatgcgga taatcagtgc gctctgacga
    12901 cctggtgtct ttatcaatca cctcactcat tgaccatgaa taaaaggctg gcacagcaac
    12961 gcagaatcct gcggtgccag cgacatgacc gcccattgct ccggtacgtt ttttaaaaag
    13021 aaacaccggt tgcattgata aattgtgctc agtatttgcc gaagtacaat atatataata
    13081 aatatataaa acacctcata ttatttacag agataataaa ttcgcgtaat tttccctctt
    13141 cagcacaaat gcctaataaa atggatcgtt cagctcacac ttccacttat taaagtcgca
    13201 aattcccaat tatgataacc ccagctttaa atgacgtatt ttgcatggat tacatattta
    13261 cgacataaat gataaatgga gatttacatg aataactctc ggttattccg tttgagcaga
    13321 attgttattg cgttaactgc cgccagcggc atgatggtaa ataccgctaa cgcgaaagag
    13381 gaagcgaaag ccgccactca atatacccaa caggttaatc agaattacgc caaatcatta
    13441 ccgtttagcg atcgtcagga ttttgacgac gctcagcgtg gatttatcgc tccgctgctg
    13501 gatgaaggta ttctgcgcga tgctaatggc aaaccatatt atcgcgggga agattataaa
    13561 tttgatatca atgcgcccgc gcccgaaacc gttaacccta gcctgtggcg tcagtcgcag
    13621 ttaaacggta tttccggcct gtttaaagtc accgacagaa tgtaccaggt tcgcggtcag
    13681 gatatctcca acatcacctt tatcgaaggt gataccggga ttattgtcat cgacccgctg
    13741 gtgacgccac cgagcgcaaa agccgccctt gacctttact tccaaaatcg cccgcaaaaa
    13801 ccgattgttg cggttattta tacccacagc cataccgacc actatggcgg cgtgaaaggc
    13861 attatctctg aagctgatgt gaaatccggt aaagtgcagg ttatcgcccc tgctggcttt
    13921 atggacgaag ccatcagcga aaacgtactg gcgggtaata ttatgagccg ccgtgcactt
    13981 tactcctacg gcctgctgct ggcgcataac cctcagggta acatcggcaa tggtcttggc
    14041 gtaacgctgg catcgggcta cccgagcatc atcgcaccga acaaaaccat caccaaaacc
    14101 ggtgagaaga tgattatcga cggcctggag tttgacttcc tgatgacccc aggtagcgaa
    14161 gcaccagccg aaatgcactt ctatattccg gccctgaaag cgctgtgtac cgccgagaac
    14221 gccacgcata ccctgcacaa cttctacact ctgcgcggcg cgaaaacccg cgacaccagc
    14281 aagtggaccg agtatctgaa cgaaacgctg gatatgtggg gtaacgacgc ggaagtcctg
    14341 tttatgccgc acacctggcc ggtctggggc aataagcata tcaatgatta tattggtaaa
    14401 tatcgcgata ctatcaagta cattcacgac cagaccctgc acctggcgaa ccagggctac
    14461 accatgaatg aaatcggcga catgattaaa ctgccgcctg cacttgccaa taactgggcc
    14521 agccgtggct attacggttc tgtcagccac aacgcccgcg cggtgtataa cttctatctt
    14581 ggctattacg acggtaaccc ggctaacctg catccgtatg gtcaggtgga gatgggtaaa
    14641 cgttacgtgc aggcgctggg cggttctgcc cgtgtcatca acctggcgca agaagcgaac
    14701 aagcaaggtg attaccgctg gtcggcagaa ctgctgaaac aggtgattgc cgccaacccg
    14761 ggtgaccagg tcgcgaagaa tctgcaagcg aataactttg aacagctggg ctatcaggcc
    14821 gagtccgcca cctggcgcgg tttctacctg accggcgcga aagagctgcg cgaaggggtg
    14881 cataagttca gccacggcac catcggttcc ccggacacca ttcgcgggat gtcggtcgaa
    14941 atgctgttcg actttatgtc cgttcgcctc gatagcgcga aagccgcggg taaaaatatc
    15001 agcctgaact tcaatatgag caatggcgat aacctcaacc tgacgctgaa cgatagcgtg
    15061 cttaactacc gtaaaacact gcaatcccaa gctgacgcct ctttctacat cagccgtgaa
    15121 gatctgcacg ccgtgctgac cgggcaagcc aaaatggcgg atctggtaaa agcgaagaaa
    15181 gccaaaatta ttggcaatgg cgcgaaactg gaagaaatta tcgcctgtct ggataatttc
    15241 gatttgtggg tgaatatcgt aaccccaaat taatgtcact cgcttttggg ggcgctgaag
    15301 cccccaaaag catttatttt aaaaatatat acagccaatt aataaataaa atatataagt
    15361 tatgaatgcg gtgactgaat aaagctattt ttattcatcg agaaaatagc catactgacg
    15421 ccttcaatgg atattgatat tcactacaga tattgttcgc agcagacagg atgtatttaa
    15481 tatgccaaca gttctctctc gcatggcgat gcaactcaaa aaaacagcct ggataattcc
    15541 cgtcttcatg gtttcgggat gctcattatc tccggcaatc ccggtgatcg gtgcttatta
    15601 tcccagctgg tttttctgcg ccattgccag ccttattttg atgctcatca cgaggcgagt
    15661 tattcagcgg gcaaatatca atctggcatt tgtcggaatt atttataccg ccctttttgc
    15721 tctctacgcc atgctgttct ggctggcatt tttctaatct atttgagacg cgctcatgga
    15781 aagtacgccg aaaaaagctc ctcgcagtaa attccctgct ctgttagtgg ttgcattggc
    15841 gctggttgcc cttgttttcg ttatctggcg cgtagacagt gcgccatcaa ctaatgacgc
    15901 ttacgcgtca gcagatacca ttgatgtggt gccggaagtc agcggccgca ttgtagaact
    15961 ggcggtcacc gacaaccagg cagtcaaaca gggcgatttg ctgttccgca ttgacccgcg
    16021 cccgtacgaa gccaatctgg cgaaagctga agcctccctc gcggcgctgg ataagcaaat
    16081 tatgctcacc cagcgtagcg ttgatgcgca acagtttggt gccgactcga ttaatgccac
    16141 ggtagaaaaa gcccgtgccg ccgcgaaaca ggccacagat acattacgcc gtaccgagcc
    16201 attactgaaa gagggttttg tctcagcgga agacgttgac cgtgcaagaa ctgcgcagcg
    16261 cgccgcagaa gccgatctta atgccgtatt gttacaggcg cagtcagccg ccagcgccgt
    16321 cagcggcgtc gatgcgctgg tggcgcaacg tgcggcggtc gaagcggata ttgccctgac
    16381 caaactgcat ctggaaatgg cgaccgttcg cgcgccgttt gatggccggg tcatttccct
    16441 caaaacctcc gtcgggcaat ttgcttctgc catgcgccct atttttaccc taatcgacac
    16501 tcgtcactgg tacgtgatcg ccaacttccg cgaaaccgat ctgaaaaata ttcgctcagg
    16561 tacacccgca acgattcgcc tgatgagtga cagcggcaaa accttcgagg gtaaagtgga
    16621 ttcgattggc tacggcgtgc taccggatga cggcggcctg gtgctgggcg gcctgccgaa
    16681 agtttctcgc tctattaact gggtccgcgt tgcccagcgt tttccggtca aaatcatggt
    16741 cgataaacct gacccggaaa tgttccgcat cggcgcttcg gcagtcgcta atcttgagcc
    16801 gcaataatga gcgcgctcaa ctccctgcca ttaccgatgg tcaggctgct ggcgttcttt
    16861 catgaagagt taagcgagcg gcgacccggc cgcgtgccgc agatcgtgca actctgggta
    16921 ggctgcctgc tggtgattct gatctcgatg acttttgaga tcccttttgt ggcgttatcg
    16981 ctggcagtgc tgttttacgg tattcagtcg aacgcgtttt acaccaaatt tgtggcgatc
    17041 ttgtttgtgg ttgccacggt gctggagatc ggcagcctgt ttttgatcta caaatggtca
    17101 tacggcgaac cgttgatccg attgatcatc gccgggccga tcctgatggg ccgcatgttt
    17161 ttgatgcgca cccatcggct gggactggtc tttttcgctg tcgccattgt cgccatttac
    17221 gggcaaacct tccccgccat gctcgactat ccggaagtgg tcgtgcgctt aacgctgtgg
    17281 tgtatcgttg ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt
    17341 cccagtcgtg ccatttcgca aatgcatcag gcgcttaatg atcggcttga tgatgccatt
    17401 agtcacctga cagacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg
    17461 gcgctgcaaa aactgaatgt cttttgtctc gcggacgatg ccaactggcg aactcaaagc
    17521 gcatggtggc aaagctgcgt ggcaacggta acctacattt actcgacgct gaatcgctac
    17581 gatcccacct cttttgctga ttctcaggca attattgaat ttcggcaaaa attagcctca
    17641 gaaatcaaca agctgcagca tgccgttgct gaaggtcagt gctggcaaag cgactggcgg
    17701 atcagtgaaa gtgaagcggt ggcggcacgg gaatgtaacc tggagaatat ctgccagacg
    17761 ttgttacaac tgggccagat gaacccgaat acgccgccaa cgcccgccgc caaaccgcca
    17821 tcaatggtcg ccgatgcttt taccaatcca gactatatac gctacgcggt aaaaacattg
    17881 ctcgcctgtt tgatctgtta caccttttac agcggcgtgg actgggaagg cattcacacc
    17941 tgtatgctga cctgcgtgat cgtctctaat ccaaatgtcg gttcgtcgta ccagaagatg
    18001 gtgctgcgtt ttggcggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg
    18061 gtcatgccct ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg
    18121 ttgggcgcat ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg
    18181 gtcaccttcg cgctcgccac gctcgaaaac gtttttggcc cagtgtacga cctggtggaa
    18241 attcgcgatc gcgccctggg tatcatcatt ggtaccgtgg tgtccgcggt gatttacacc
    18301 tttgtctggc ctgaaagtga agcgcgcacg ctgccgcaaa aactggctgg cgcgctgggt
    18361 atgttaagta aagtaatgcg gatcccacgc cagcaggaag tcacggctct gcgcacttat
    18421 ctgcaaattc gtatcggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc
    18481 gtggcgctgg agcgtcaact ggacagcgaa gaacgcgcct tactgattga acgttcgcaa
    18541 acggttattc gtcagggccg cgatatcctt cacgcctggg atgccacctg gaattcggcg
    18601 caggcgctgg ataacgcact acagccggac agagcaggtc agtttgccga cgccctggaa
    18661 aaatacgctg ccggtctggc aaccgcactc agccgttctc ctcaaataac gcttgaagag
    18721 acacccgcct cgcaggccat cctgcccacc ttattaaaac aggagcaaca cgtctgccag
    18781 cttttcgccc gcttgccaga ctggacagcc ccggcattaa cgcccgccac ggaacaggca
    18841 caaggagcca cgcaatgatc aatcgtcaac tttcacgtct gctgttgtgc agcattctcg
    18901 gcagcacgac gctgatttcc ggctgtgcct tggtacgcaa ggattctgcg cctcatcaac
    18961 agctcaaacc ggaacaaatc aaactggccg atgatattca tcttgccagc tccggctggc
    19021 cgcaggcgca gtggtggaaa caactcaatg acccgtagct ggatgcgctg atccaacgga
    19081 cgctaagtgg ttcacacacc ctcgccgaag cgaaactgcg ggaagaaaaa gcgcagtccc
    19141 aggccgattt gttagatgcc ggttcacaat tacaggtcgc agcgttaggg atgctcaacc
    19201 gccaacgcgt ctcggcgaac ggctttttaa gcccttatgc gatggatgcg cccgcactgg
    19261 gtatggacgg gccgtactat acggaagcca cagtaggttt gtttgccgga ctggatcttg
    19321 atttgtgggg tgtgcatcgc tcagcggttg ccgccgccat tggcgcgcat aatgccgcgc
    19381 tggcagaaac cgcagcagta gagctatcgc tgaccacggg cgtagcgcag ctttattaca
    19441 gtatgcaggc cagctatcag atgctcgatc tgttagaaca aactcgcgat gtgattgatt
    19501 acgcggtgaa agcgcaccag agtaaagtgg cgcacggtct ggaagcacaa gtgcctttcc
    19561 acggcgcgcg ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc aaagggcaaa
    19621 tcaccgaaac gcgagaatct ctgcgtgcat tgattggcgc gggggccagc gatatgccgg
    19681 agatcaaacc ggtggcatta ccacgagtcc agaccggcat tccggcgaca ctctcttatg
    19741 agttgctcgc cagacgcccg gatctgcaag ccatgcgctg gtatgttcag gcgtcattag
    19801 atcaggtgga ttccgcgcgg gcgctgttct acccgagctt tgatatcaaa gcgtttttcg
    19861 gccttgatgc catccacctg gataccttat tcaaaaaaac cagtcgccag ttcaacttca
    19921 tcccgggtct gaaattgccg ctgtttgacg gtggacggtt gaatgccaat ctcgaaggca
    19981 cgcgcgccgc cagcaacatg atgattgaac gttacaacca gtcagtactg aacgcggtgc
    20041 gtgacgttgc cgtcaacggc acgcgtctgc aaacgctcaa cgacgagcga gaaatgcagg
    20101 ctgaacgcgt ggaagcaacg cgcttcaccc agcgcgctgc cgaggccgcc tatcagcgcg
    20161 gcttaaccag ccgcttacag gccactgaag cccggttgcc agtacttgcc gaagagatgt
    20221 cattactgat gctggacagc cgccgggtga tccaaagcat tcagttgatg aaatcgctgg
    20281 gcggcgggta tcaggcggct cccgtcgtcg agaaaaaata aaatgtctgc cgcgtgatgg
    20341 ctgtcacgcg gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt tttcgtcaga
    20401 gttttgaata aatagtgccc gtaatatcag ggaatgaccc caaataaaat gtggcataaa
    20461 agatgcatac tgtagtcgag agcgcgtatg cgtgatttga ttaactggag cgagaccgat
    20521 gaaaaaagtc gtcacggttt gcccctattg cgcatcaggt tgcaaaatca acctggtcgt
    20581 cgataacggc aaaatcgtcc gggcggaggc agcgcagggg aaaaccaacc agggtaccct
    20641 gtgtctgaag ggttattatg gctgggattt cattaacgat acccagatcc tgaccccgcg
    20701 cctgaaaacc cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg tttcctggga
    20761 tgaggcactg aattacgttg ccgagcgcct gagcgccatc aaagagaagt acggtccgga
    20821 tgccatccag acgaccggct cctcgcgtgg tacgggtaac gaaaccaact atgtaatgca
    20881 aaaatttgcg cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc gtgtctgaca
    20941 cggcccatcg gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga gcaatgctat
    21001 taacgaaatt gataataccg atttagtgtt cgttttcggg tacaacccgg ctgattccca
    21061 cccaatcgtg gcgaatcacg taattaacgc taaacgtaac ggggcgaaaa ttatcgtctg
    21121 cgatccgcgc aaaattgaaa ccgcgcgcat tgctgacatg cacattgcac tgaaaaacgg
    21181 ctcgaacatc gcgctgttga atgcgatgga ccatgtcatt attgaagaaa atctgtacga
    21241 caaagcgttc gtcgcttccc gtacagaagg ctttgaagag tatcgtaaaa tcgttgaagg
    21301 ctacacgccg gagtcggttg aagatatcac cggcgtcagc gccagtgaga ttcgtcaggc
    21361 ggcacggatg tatgcccagg cgaaaagcgc cgccatcctg tggggcatgg gtgtaaccca
    21421 gttctaccag ggcgtggaaa ccgtgcgttc tctgaccagc ctcgcgatgc tgactggtaa
    21481 cctcggtaag ccgcatgcgg gtgtaaaccc ggttcgtggt cagaacaacg tgcagggtgc
    21541 ctgcgatatg ggcgcgctgc cggatacgta tccgggatac cagtacgtga aagatccggc
    21601 taaccgcgag aaattcgcca aagcctgggg cgtggaaagc ctgcctgctc ataccggtta
    21661 tcgcatcagc gagctgccgc accgcgcagc gcatggcgaa gtgcgtgccg cgtacattat
    21721 gggcgaagat ccgctgcaaa ctgacgcgga gctgtcggca gtacgtaaag cctttgaaga
    21781 tctggaactg gttatcgttc aggacatctt tatgaccaaa accgcgtcgg cggcggatgt
    21841 cattttgccg tcaacgtcgt ggggcgagca tgaaggcgtg tttactgcgg ctgaccgtgg
    21901 cttccagcgt ttcttcaaag cagttgaacc gaaatgggat ctgaaaacgg actggcaaat
    21961 catcagtgaa atcgccaccc gtatgggtta tccgatgcac tacaacaaca cccaggagat
    22021 ctgggatgag ttgcgtcatc tgtgcccgga tttctacggt gcgacttacg agaaaatggg
    22081 cgaactgggc ttcattcagt ggccttgccg cgatacttca gatgccgatc aggggacttc
    22141 ttatctgttt aaagagaagt ttgatacccc gaacggtctg gcgcagttct tcacctgcga
    22201 ctgggtagcg ccaatcgaca aactcaccga cgagtacccg atggtactgt caacggtgcg
    22261 tgaagttggt cactactctt gccgttcgat gaccggtaac tgtgcggcac tggcggcgct
    22321 ggctgatgaa cctggctacg cacaaatcaa taccgaagac gccaaacgtc tgggtattga
    22381 agatgaggca ttggtttggg tgcactcgcg taaaggcaaa attatcaccc gtgcgcaggt
    22441 cagcgatcgt ccgaacaaag gggcgattta catgacctac cagtggtgga ttggtgcctg
    22501 taacgagctg gttaccgaaa acttaagccc gattacgaaa acgccggaat acaaatactg
    22561 cgccgtgcgc gtcgagccga tcgccgatca gcgcgccgcc gagcagtacg tgattgacga
    22621 gtacaacaag ttgaaaactc gcctgcgcga agcggcactg gcgtaataca gtcctttcta
    22681 cagcctcctt tcggaggctg tttttttatc cattcgaact ctttatactg gttactccct
    22741 acccaatcgt attatcaaaa tgaaaaaaat tatcgcattg atgttgtttt tgacattctt
    22801 tgcccacgcc aacgactccg agcctggcag ccagtattta aaggcagcag aggccgggga
    22861 ccgacgcgca caatattttc ttgccgacag ctggtttagc tccggcgatt tgagcaaagc
    22921 cgaatattgg gcacagaaag ccgccgacag cggtgatgct gatgcctgcg cgctgctggc
    22981 gcagatcaaa atcaccaatc cggtcagtct ggactatcca caagcaaaag ttcttgcaga
    23041 gaaagcggcg caagcgggca gtaaagaagg tgaagtaacg ctggcgcata ttctggtaaa
    23101 tactcaagcg ggtaaaccgg attatccaaa ggctatttcg ctgttagaaa acgcctcgga
    23161 agatctggag aacgactctg ccgtcgatgc ccaaatgctg cttggtttga tttacgccaa
    23221 cggcgtgggc attaaggccg acgatgacaa ggcaacctgg tatttcaaac gcagctctgc
    23281 aatttcccga accggttatt ccgagtactg ggcgggaatg atgttcttaa acggtgaaga
    23341 aggttttatc gagaagaaca agcaaaaggc gctgcactgg ttgaacctga gctgtatgga
    23401 agggtttgat accgggtgtg aagagtttga aaaattaacc aacggttaag gtaggcctga
    23461 taagacgcgg caagcgtcgc atcaggcatt cacacattgc cgggtggata tccccggcaa
    23521 tcttcaatta ttgatccgca gttttatcaa atttgcccag cacttcacgc tcataagcca
    23581 gcgccttttt gcggtcaaat ttgtgttccc acttggcaat caccagcacc gccagcgcat
    23641 tacccaccac gttcagcgca gtacgcgcca tgtcgaggat acggtcaaca ccagcaataa
    23701 acgccagacc ttccagcgga atacctacgc tacccagcgt tgccagcagc accacaaacg
    23761 acacgcccgg cacgccagca atccctttcg aggtcaccat cagcgtcagc accagaatga
    23821 tttcctgcca gatggacagg tcaatgccat acagctgagc gatgaaaata gcggcaatac
    23881 tttgatacag cgtcgaacca tcaaggttaa aagagtaacc ggtcggcacc acgaagctgg
    23941 tgatcgacgc cggtgctccg taggcttcca tcttctcaat aattcgcggc agtacgcttt
    24001 cagagctggc agtggagtac gccagaatca gctcatcttt cagaatacga atcaggatcc
    24061 agacgcttaa cccgcacagg cgcgccacaa ttcccagcac taccagcgcg aagaacagaa
    24121 tggcgaaatg caccagcagc accagtttcg ccagtggcca cagagaagag aaaccaaagt
    24181 tagccaccgt caccgcaatc agcgcaaaca cacccaccgg tgcataacgc atcaccatgt
    24241 gagtcacttt aaacatggtt tcagagatgg agcggaacac ggtcaccagc agttcacgat
    24301 gcgtcgcggg cagggaagaa agccccagac caaacagcac cgagaaaaag atgatcggca
    24361 gcatttcgcc tttcgccatc gacgccacaa tgttcgtcgg caccagcgac aaaatcgtgc
    24421 ccataatgcc gtgggaactg ctttgtaccg cttccgtagt gctctgatat ttcgagatat
    24481 cgacggtcgc cagctgcgac atatccaccc cggcaccggg ctggaagacg ttcgccagag
    24541 tgatccccaa aatgatggcg acggtggtga tcacctcgaa gtaaataatg gttttcgcgc
    24601 caatacgccc gagctgtttg gcatcaccaa cacccgcgat acccaccacc agcgtggaga
    24661 tcacaatcgg cacaacaatc attttgatca gatggatgaa gatatcaccc gccggagaga
    24721 gcaaattgac gaccagccag tcgcggctgt cgctatggta gtgcaggtag cttcccagga
    24781 gaatgcccag caccatagca aacagaatct gccaggccag gctgaatttt atatttttca
    24841 tcagaataac ttcctcaatg gagcgccgtt ccccgttcag aaacgcgaga acgtgacata
    24901 ctgccagggc atgaattgtg ttgcgttggt ttgtagattt ttttaacgct tatgatcagt
    24961 atcatttgcg cggtatgttg cgcaaccctt taacaacaga gcttttcgct gcattaatgc
    25021 ataataacgc gagtttatgg caggattaat gaataaccat ttgttatgga aagattttta
    25081 tttgtttaat ctgcaataat actgattagt gattattttt ggcatatgtt cattcgatta
    25141 ttttttatgc agctcaatga cataatcttc atcaccagat gattaatccg atctccgctg
    25201 caacagtttt gccatattaa tcgactcgac cagctgtgtc cggttgatcc ggggtgagtt
    25261 gagatccatc actttttgcc atagttcgat ggcttgcgcg taattcgcct gcataaacgc
    25321 atcggaagcc agcagcatca gagcggtgat ttcgttagag tccagcgcga gggctttgtc
    25381 gatcattgcg caagtctggg cggtcatatg ctggctggcc tggtaataaa gcaccgtcgc
    25441 cagcgccgca tacagttcag cgttctcacc acgtagttgc aacgcctgac gatacgccag
    25501 cagcgaattg ctgtaatcgt tttgccacag atagtactcg cccagtaacg cccattgttc
    25561 gctgttttgt ggattagcgc ggattttatc ctgcaatgcc tgaagctgcg cttccggggt
    25621 ttgctggctg gcaaactgat gtagcggatc gcgctgacgc tgatactccg cacgtaccgc
    25681 ctgccatttt gggcttaaca gataactgcc gacacagagg aataccatca atatcgtgag
    25741 tgttgtcagc attttcaccg gtatttgagg ctgtttcatc gcttagccct cactcgccag
    25801 aggatcagtg ccatcagcaa taacaacacc actggcagcg cccacaacac cagcgtctga
    25861 cccgttaacg gtgggttata gcgaacaaaa tctccgtagc gttcggtcat ccagccaatg
    25921 atttcgactt cgttcttccc ctccgacacc atgctataaa cctgatggcg catactgacc
    25981 gccaccggcg cattggattc gagtaagttt tgattctggc actgcggaca acgtaactgg
    26041 ctggcaatat ttaacgcctg ttgctgttgt tgcggattgg cgaattgcca ggtgtctacg
    26101 acctgagcgc gcgcaaaaca ggtaaataac agcagtagcg tgagaagccc tttattcatc
    26161 gcgcttcttc ccccgccatc cgcttaacaa tgcgcccgca atcatcaaca atcctccacc
    26221 ccagatccag cgcacaccgc tttgtacata caaacgaaaa gcgtaacgat ccgctccggt
    26281 tttttcaccc atgaccgcat accagtcatg gatgccgttc cagcgaattg acggttccat
    26341 catttgctga cggcgtgctt cataaaaacg ccgctccggc gttaattcac caatgcgttg
    26401 ctgatggtca aacagcgcca ctatcgcttt ttcgctggtg taattgcctt tggcttgcag
    26461 atcgaggcgc tcaaaacgga aggtgtatcc tgccagcgtc acctgctgac ccggctgtaa
    26521 attcaggctg atctcctgac ggctgacgct ggaaaccacg atccctgcgg caaataacag
    26581 cacgcccgca tgagcgacca gcgccggaag ctgcgcgcgt ttgccagaga caaacgtcgc
    26641 cagcacaatc accaccagca tcaacagacc aaacggtaac gtcgcgcggt taaaatacgg
    26701 cgcgccaacg gagaggcgtc cccagcccag cagaccgtaa atcatcggat aaagcgttcc
    26761 caccagcacg atcagtagca ctgcgcaaaa cagcaacagc gtagcgagga ttaacatttc
    26821 ccgcgataac cccgaaaaac gcaccgccgg gccaccgtcc cgcgctcgcc agccatacag
    26881 agccaaagac gcaaggctaa tcagtgcaaa caggctgaac aacggcacgg cgcggacgtt
    26941 atccagtgcg aacgcatgaa ccgaaaccag aatgccagaa cggacaatta aggtgcccag
    27001 cagcgacagc atcagagtaa ctatcgccag caacagcgac cagtggcaaa aaatcccccg
    27061 ctggcgtgtc agggataaac tgtgcagcag cgcagtggca gaaagccagg gtaataaaga
    27121 ggcattttcc accggatccc agaaccacca gccgccccag ccgagttcgc agtaggccca
    27181 ccaggaaccg aggatgatcc ccgccgttaa tgcactccag ccaggtaacg cccagcgcca
    27241 gcaaattcgg gcgctggccg catcaaactc gccgcgcagt agactcgcca gcgccacgct
    27301 cgccgctacc atcaaaccgc catagccaag gtaaagcagc ggtggatgaa agataagacc
    27361 gggatgttgc agcatcggat tgagatcgcg gccttcgatt gctggtggaa atatccgcac
    27421 gaagggatcg gaccacagca ccacaaacag tagcagtgcg gcgagcatga gagataaaac
    27481 ggctaacgtc agcggaaata gcggatcggt ttgctgccga taatgccagg caaacagcgc
    27541 gctccaggcg gaaagcagca gcacccaaag cagcagcgaa ccttcatggc cgccccacac
    27601 cgctgccagc ttgaattccc acgacagcag gctatagcta tgttgcgcga cataaatgac
    27661 cgagaaatcg ctgatgagaa aacaatacgt cagtacgcca aaggcgagca gcaggagcgc
    27721 gaactgcgcc agaatgccga tgcaagtgag tcgcatcatg gcaggccagc gcaaccgcac
    27781 tcccgcaaag gccgtcaacg gggtcaacac gttgaccccg agacttaaca acaacgccag
    27841 aaaaccgact tcaggaagga atacgtccag aaaccacctc ttttctggct tacgccaccg
    27901 tcaactgtcc ggcgtagaga atgaaaaagc gcagcatcaa cacgccgacc agactggccc
    27961 cacacgccgc cagcacggca ggaatgccgg aactgcgatt gacccacggt ttgagcaaca
    28021 ttggcacaat cagccccagc ccggcgacac caagccagaa ccaccacgtc cagaaaccgc
    28081 cacctaatgc cgccaccagc gcacgaactt taccgtcatc gcccagcgcc agaccgacaa
    28141 aaaacgccac cagcaggaag atttcacccc ataccacggg gatttccatc cggtgaacaa
    28201 agtgcgcttc cgtggaatgc gggttactgc gttggcgtat cgccatggcg atcagcgcca
    28261 ccgccgcacc ggacgagatg ccggagaaga ggaacagcac cggcaggatc gggttattga
    28321 ggaacggata cgatttcagc gccgacagca gaaagccggt ataagccccc agcagcaccg
    28381 ccaacaccag catcagcgtt tccagtccgc gatgaacggg cgtcagcagg ctcagaacct
    28441 tttgcacgat ccccagcttc ggcaaccagc gttgttgcag ggcaagcaaa tcatgttcaa
    28501 agattttcgc cagccacagc accagcacca ccatgtagag ctgaaacagc atcaccccca
    28561 tcgacatcac cgaggtaaaa ctgtagtgga acatcagctt ccagaaggtc cacggtcttg
    28621 tcaggtggaa gacgaggatc aacagaccga ggatcaccgc gcccggcccg acaatcagcg
    28681 tggtgcgcag caacgtactg tctgcaccgc ccgcctgcgg gtagaagcga cgtaacagca
    28741 cggccagcgt caccagaccg gcagaaatac caatcaaaaa caggtagatg gcaatcggcc
    28801 agtcccacac cagcgattca aaatgaaatg cggaagtctg cgtcattggc tcacctcccc
    28861 gtatttaaac ggtacgcggt acagcttcgg tttggttccc agcgccagct tgtagcggta
    28921 agtaggcttc tggcgcagca gttgcgaaat ctcactgtta ggatcgtcca gattgccaaa
    28981 cgtcagcgcc ttcgtcgggc aagcctcaac acacgcgggc agcttaccgg cctgcaaatt
    29041 ggttttacgg cagaaatcgc atttgtccgc cgtcttcgtg accggatgga taaagcgcac
    29101 gcggtacgga caggcggcga tgcagtactg acaaccgacg cagagatcag gattaacatc
    29161 gacaatcccg ctagcggcat cacgaaaaga cgcaccggtc gggcagacgt caacgcacgg
    29221 cgcatgatcg cagtgctggc aagacttacg gaagaagcga tatttcacat caggaaattc
    29281 gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg ccttccggca ccttgttcac
    29341 ttcccgacaa gcatccatac aggcggtaca gccgatgcat aacgactcgt catgcaccat
    29401 gccgtaacgc accccattga tattcaacgt tttcgccacc acgcgccctg cggtgccgct
    29461 gacggctgcc agcacgccga cgccggtgag aaactggcga cgagaccagg tcatggctgc
    29521 tccttaagca acggaacaga cgccgggtta aagttcggat tggtgcgctg atcgctgtgg
    29581 caatcgacgc aaatcttaat ccgtcctttg tcacttaacg tctgcatcgt atcttgctgt
    29641 ggatgcaggg aatggcagct ggcgcacgcc actttggtta cgtggacatc gtgcggccag
    29701 aacgcttttt gcaactgttc cggcagatga caggacatac agacgctgtt ctgctctcca
    29761 accttgtaca tcggctcgtt aaagcgcatc acatctttca ccccttcgcg gtgttgcggt
    29821 gatggctggc cgtgacagtt ggtgcaggtg accggcagtt tgttattcgg gttgataacg
    29881 gaagcatgtt tgccatgcat accttcggta tctggtttat gacagtccag acaggcggca
    29941 tccgggttac gctgctgggt aacttcgtag cgatcatcag atgcctgcgc cgcaggggta
    30001 gcggtaattc cgttcaggct ccacaacagg cccgacgcca gcaccccggc agttaataac
    30061 gaacgtaata cgctcatatt cactccattg tgttttccgc cccttgcgag gcggaacggg
    30121 gttattggct taacagaccg tttttacgtg cctgttcttc ccactgcggg atcaccgttt
    30181 tgatgaagtc ctgcttctcg gccttgattt gttccatgtt caggccaatg gcctgctggg
    30241 ctttctcttt ggttgagata tccgggatct ggatttcatg ggtgatgcct ttggtcgcca
    30301 gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc catcgccgta ccgagcatcc
    30361 gtaaaccttc ttccggtgcg tgcatatgaa tgccgtggga agcgatcgcc agatcccagc
    30421 gccactgggc atgacggata tcgtcctgaa ttggcttcat ttcagcttcc gtcgcgcctg
    30481 catccagcgc tgctttcgct tcgaagtgag cgtgaaccag ttgatcttca accttgattt
    30541 tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt ttgcaaggca gctttgtcct
    30601 gggtatggca gttcgcacaa gtctgggcga agttatcaaa cggattacca attttatggt
    30661 cggtatagag tttgccttcg gcgttctgca cttttggcat atggcagtcg atacaggtca
    30721 cgttgttttt accgtgaatg cccgctgtcc aggtttcata ttccgggtgc tgcgctttca
    30781 gcattggcgt tttcgacagg gagttagtcc agtcagagaa ggcaattttg tcgtaatact
    30841 gctccatatt ttcgactttc atgccgtcat cccacgggaa tttaaccgct ttgtttttgc
    30901 cgtcgaagta atactccaca tggcactgac cgcaaaccat cgattgctgg tcgaaacgtc
    30961 cggctttctc aaaaggttta ccaatggctt ccatcgcgcg agccgcatac ggacgggaaa
    31021 gggttaactc cggtttgcct ttggcgaact ctggtgaggc ggtgttatgg cagtcggcac
    31081 aacctaagtt gttgacgatt tccgggccgc cgcgcgccca cttaccgtgg aagtagccat
    31141 cttcgccgtc tttctggatc agacgcgcca catccgggct tttacaactc cagcacgcca
    31201 tcggtagcgg accatcttca gcgtttttcg gcgcaccggt acgcagggtt tcacgcacat
    31261 cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc gcgcgagaag ggataccccg
    31321 cccacaggat caccagccgt ggatcttccg ccagggcgtc aacacgctct gactgttccg
    31381 aggtggcttt ccaggagaga tattgatcgg gatgctgcgg ggcaaaggtt tcattcttcg
    31441 cttccacagt tacaggtttt gcgggagctg ccgtttgttc agcgtgaaca gaagtgaaaa
    31501 agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt tatttttatc cttgtcatag
    31561 gggcttcatc cgaattgcgc cattgttaca atggcggttt ttattgtttt tcacgacagt
    31621 aaccgcacct acactgtcat gacattgctc gcccctatgt gtaacaaata accacactgt
    31681 gaatgttgtc tttaatcaat tgtaagtgca tgtaaaatac cactttagag ttagttagta
    31741 tcttcctctt tttcaacagc aatgcataac tgcatgttcc tcaaagaatt aatcaacttt
    31801 tgttgctgac cttcaaaaat taccctgccg tttatttgca caattctact tttgcgtgat
    31861 ctgtcgccca aatactaaac aaaactgcca atacccctac atttaacgct tatgccacat
    31921 attattaaca ccctacaagg agaacaaaag catgagccaa attcacaaac acaccattcc
    31981 tgccaacatc gcagaccgtt gcctgataaa ccctcagcag tacgaggcga tgtatcaaca
    32041 atctattaac gtacctgata ccttctgggg cgaacaggga aaaattcttg actggatcaa
    32101 accttaccag aaggtgaaaa acacctcctt tgcccccggt aatgtgtcca ttaaatggta
    32161 cgaggacggc acgctgaatc tggcggcaaa ctgccttgac cgccatctgc aagaaaacgg
    32221 cgatcgtacc gccatcatct gggaaggcga cgacgccagc cagagcaaac atatcagcta
    32281 taaagagctg caccgcgacg tctgccgctt cgccaatacc ctgctcgagc tgggcattaa
    32341 aaaaggtgat gtggtggcga tttatatgcc gatggtgccg gaagccgcgg ttgcgatgct
    32401 ggcctgcgcc cgcattggcg cggtgcattc ggtgattttc ggcggcttct cgccggaagc
    32461 cgttgccggg cgcattattg attccaactc acgactggtg atcacttccg acgaaggtgt
    32521 gcgtgccggg cgcagtattc cgctgaagaa aaacgttgat gacgcgctga aaaacccgaa
    32581 cgtcaccagc gtagagcatg tggtggtact gaagcgtact ggcgggaaaa ttgactggca
    32641 ggaagggcgc gacctgtggt ggcacgacct ggttgagcaa gcgagcgatc agcaccaggc
    32701 ggaaaagatg aacgccgaag atccgctgtt tattctctac acctccggtt ctaccggtaa
    32761 gccaaaaggt gtgctgcata ctaccggcgg ttatctggtg tacgcggcgc tgacctttaa
    32821 atatgtcttt gattatcatc cgggtgatat ctactggtgc accgccgatg tgggctgggt
    32881 gaccggacac agttacttgc tgtacggccc gctgacctgc ggtgcgacca cgctgatgtt
    32941 tgaaggcgta cccaactggc cgacgcctgc ccgtatggcg caggtggtgg acaagcatca
    33001 ggtcaatatt ctctataccg cacccacggc gatccgcgcg ctgatggcgg aaggcgataa
    33061 agcgatcgag ggtaccgacc gttcgtcgct gcgcattctc ggttccgtgg gcgagccaat
    33121 taacccggaa gcgtgggagt ggtactggaa aaaaatcggc aacgagaaat gtccggtggt
    33181 cgatacctgg tggcagaccg aaactggcgg tttcatgatc acgccgctgc ctggcgctac
    33241 cgagctgaaa gccggttcgg caacacgtcc gttcttcggc gtgcaaccgg cgctggtcga
    33301 taacgaaggt aacccgctgg aaggcgctac cgaaggcagc ctggtgatca ccgactcctg
    33361 gccgggtcag gcgcgtacgc tgtttggcga tcacgaacgt tttgaacaga cctacttctc
    33421 caccttcaaa aatatgtatt tcagcggcga cggcgcgcgt cgcgatgaag atggctatta
    33481 ctggatcacc gggcgtgtgg acgacgtgct gaacgtctcc ggtcaccgtc tggggacggc
    33541 agagattgag tcggcgctgg tggcgcatcc gaagattgcc gaagccgccg tagtaggtat
    33601 tccgcacaat attaaaggtc aggcgatcta cgcctacgtc acgcttaatc acggggagga
    33661 accgtcacca gaactgtacg cagaagtccg caactgggtg cgtaaagaga ttggcccgct
    33721 ggcgacgcca gacgtgctgc actggaccga ctccctgcct aaaacccgct ccggcaaaat
    33781 tatgcgccgt attctgcgca aaattgcggc gggcgatacc agcaacctgg gcgatacctc
    33841 gacgcttgcc gatcctggcg tagtcgagaa gctgcttgaa gagaagcagg ctatcgcgat
    33901 gccatcgtaa cccacaattg ccggatgcga gtcggtaacg gtttgtaggc ctgataagac
    33961 gcgacagcgt cgcatcaggc attgattgcc ggatgcggcg taaacgcctt atccggccta
    34021 cattcggcaa gggttacccg agcgttaacc ttctcccata agggagcggg aattaaaaca
    34081 atccctacat tacctctgga gaatctgtga tgaatggcac tatttatcag cggatagaag
    34141 acaatgcgca tttcagggag ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga
    34201 ttattatgct ggcagtttat atcggcttta ttttactgat cgccttcgcg cccggctggc
    34261 tggcacgccg ctgaatccga acaccagcgt cacacgcggt attccaattg gtgttggagt
    34321 gattgtgatc tcctttgttc tcaccggtat ctacatctgg cgggcgaacg gcgaattcga
    34381 ccgtcttaat aatgaagtcc tgcatgaggt acaagcatca tgaaaagagt tctgacggcg
    34441 cttgccgcca cactcccttt cgcagctaac gccgcggatg ctattagcgg ggccgtagag
    34501 cgccagccaa cgaactggca ggcgattatt atgttcctga ttttcgtcgt gtttacgctc
    34561 ggcattacct actgggcatc aaaactcgta cgttctcgta gcgactacta caccgcaggc
    34621 ggcaatatca ctggcttcca gaacgggctg gcgattgccg gggactatat gtccgccgcc
    34681 tcattcttgg ggatctccgc gctggtgttt acctccggct atgacggctt aatttactcg
    34741 ctgggcttcc tggtgggctg gccgatcatt ttgttcctga ttgccgaacg tctgcgtaac
    34801 ctggggcgct acacctttgc cgatgtggcc tcttaccgtc tgaaacaagg gccgattcgt
    34861 attctttcgg cctgtggttc tctggtggtg gtggcgcttt accttatcgc ccagatggtg
    34921 ggcgcaggta aactgatcga gctgctgttt ggccttaact atcacattgc ggtggtgctg
    34981 gtcggcgtgc tgatgatgat gtacgtcctg ttcggcggca tgctggcgac cacttgggta
    35041 cagattatca aagccgtgct gttgctgttc ggtgccagct ttatggcctt tatggtgatg
    35101 aaacacgtcg gctttagctt caacaatctg ttcagtgaag cgatggcggt acacccgaaa
    35161 ggtgtcgaca tcatgaagcc gggcgggctg gtgaaagatc cgatctccgc actctctctg
    35221 ggtctgggac tgatgtttgg tacggcgggc ttgccgcaca ttctgatgcg cttctttaca
    35281 gtcagcgatg cccgcgaagc acgtaagagc gtgttctacg ccaccgggtt tatgggctac
    35341 ttctatattc tgacctttat tatcggcttc ggcgcgatca tgctggttgg tgcgaatccg
    35401 gaatataaag acgcggcggg ccatctgatt ggtggtaaca acatggcggc cgttcacctg
    35461 gcgaatgcag tgggcggcaa cctgttcctc ggttttattt cagcggttgc tttcgccact
    35521 atcctcgcgg tggttgcggg tctgacgctg gcgggcgcat ccgcggtttc gcatgacttg
    35581 tacgctaacg tcttcaaaaa aggcgcgacc gaacgtgaag agctgcgggt atcaaaaatc
    35641 accgtactga tcctcggcgt gattgcgatt atcctcggcg tgctgtttga gaatcagaac
    35701 atcgccttta tggtggggct ggcgtttgcc atcgcggcga gctgtaactt cccgatcatt
    35761 ctgctttcta tgtactggtc gaaactgacc acgcgtggcg cgatgatggg tggctggctg
    35821 gggctgatta ccgcagtagt actgatgatc ctcggcccga cgatttgggt acagatcctt
    35881 ggtcacgaaa aagccatctt cccgtatgaa tacccggcgc tgttctctat caccgtggca
    35941 ttcctcggca tctggttctt ctcggcaacc gataactcag cggaaggggc gcgcgagcgt
    36001 gaactgttcc gcgcgcagtt tatccgctcc cagaccggct ttggcgttga gcaaggccgc
    36061 gcacattaat ctctcccctt ccccggtcgc ctgaccgggg aatactcttc ctctccagca
    36121 tgcatcacct tttcccaaaa tattaaacaa ataaactcat taaaaaatga gcgatttttg
    36181 acagtcgtag aaaatgata
//
LOCUS       DAZPJP010000023        34983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPJP010000023 DAZPJP010000000
VERSION     DAZPJP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.023"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHU9J_001021"
     CDS             complement(<1..815)
                     /locus_tag="ACHU9J_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1904625.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHU9J_001022"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHU9J_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHU9J_001023"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHU9J_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHU9J_001024"
     CDS             complement(1397..1873)
                     /locus_tag="ACHU9J_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1904626.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHU9J_001025"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHU9J_001025"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1904627.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHU9J_001026"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHU9J_001026"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1904628.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHU9J_001027"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHU9J_001027"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1904629.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHU9J_001028"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHU9J_001028"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1904630.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHU9J_001029"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHU9J_001029"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1904631.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHU9J_001030"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHU9J_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1904632.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHU9J_001031"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHU9J_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1904633.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHU9J_001032"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHU9J_001032"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1904634.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHU9J_001033"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHU9J_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1904635.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHU9J_001034"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHU9J_001034"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1904636.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHU9J_001035"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHU9J_001035"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1904637.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHU9J_001036"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHU9J_001036"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1904638.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHU9J_001037"
     CDS             13839..14543
                     /locus_tag="ACHU9J_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1904639.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14814..15461
                     /locus_tag="ACHU9J_001038"
     CDS             14814..15461
                     /locus_tag="ACHU9J_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1904640.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(15526..16237)
                     /locus_tag="ACHU9J_001039"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="ACHU9J_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHU9J_001040"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHU9J_001040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1904641.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="ACHU9J_001041"
     CDS             complement(17475..18236)
                     /locus_tag="ACHU9J_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1904642.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="ACHU9J_001042"
     CDS             18369..18779
                     /locus_tag="ACHU9J_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1904643.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="ACHU9J_001043"
     CDS             complement(18741..19847)
                     /locus_tag="ACHU9J_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1904644.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="ACHU9J_001044"
     CDS             complement(19858..21000)
                     /locus_tag="ACHU9J_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1904645.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHU9J_001045"
     CDS             complement(20993..22729)
                     /locus_tag="ACHU9J_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1904646.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHU9J_001046"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHU9J_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1904647.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHU9J_001047"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHU9J_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1904648.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHU9J_001048"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHU9J_001048"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1904649.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHU9J_001049"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHU9J_001049"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1904650.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHU9J_001050"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHU9J_001050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1904651.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHU9J_001051"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHU9J_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1904652.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHU9J_001052"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHU9J_001052"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1904653.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHU9J_001053"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHU9J_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1904654.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHU9J_001054"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHU9J_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1904655.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHU9J_001055"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHU9J_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1904656.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHU9J_001056"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHU9J_001056"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1904657.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34983)
                     /locus_tag="ACHU9J_001057"
                     /pseudo
     CDS             complement(32997..34983)
                     /locus_tag="ACHU9J_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8270 a         8978 c         9491 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 ccg
//
LOCUS       DAZPJP010000024        34602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPJP010000024 DAZPJP010000000
VERSION     DAZPJP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.024"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /locus_tag="ACHU9J_001058"
     CDS             complement(<1..459)
                     /locus_tag="ACHU9J_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1904658.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
     gene            complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHU9J_001059"
     CDS             complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHU9J_001059"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1904659.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHU9J_001060"
     CDS             complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHU9J_001060"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1904660.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHU9J_001061"
     CDS             complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHU9J_001061"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1904661.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(3073..3510)
                     /locus_tag="ACHU9J_001062"
     CDS             complement(3073..3510)
                     /locus_tag="ACHU9J_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR1904662.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            complement(3611..4111)
                     /locus_tag="ACHU9J_001063"
     CDS             complement(3611..4111)
                     /locus_tag="ACHU9J_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904663.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            complement(4114..5052)
                     /locus_tag="ACHU9J_001064"
     CDS             complement(4114..5052)
                     /locus_tag="ACHU9J_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1904664.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            complement(5141..5452)
                     /locus_tag="ACHU9J_001065"
     CDS             complement(5141..5452)
                     /locus_tag="ACHU9J_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1904665.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            5548..5826
                     /locus_tag="ACHU9J_001066"
     CDS             5548..5826
                     /locus_tag="ACHU9J_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1904666.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            5841..6179
                     /locus_tag="ACHU9J_001067"
     CDS             5841..6179
                     /locus_tag="ACHU9J_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR1904667.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            6190..6477
                     /locus_tag="ACHU9J_001068"
     CDS             6190..6477
                     /locus_tag="ACHU9J_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904668.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            6489..6731
                     /locus_tag="ACHU9J_001069"
     CDS             6489..6731
                     /locus_tag="ACHU9J_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR1904669.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            6728..6841
                     /locus_tag="ACHU9J_001070"
     CDS             6728..6841
                     /locus_tag="ACHU9J_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904670.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            6928..7131
                     /locus_tag="ACHU9J_001071"
     CDS             6928..7131
                     /locus_tag="ACHU9J_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904671.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            7128..7373
                     /locus_tag="ACHU9J_001072"
     CDS             7128..7373
                     /locus_tag="ACHU9J_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1904672.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            7399..7518
                     /locus_tag="ACHU9J_001073"
                     /pseudo
     CDS             7399..7518
                     /locus_tag="ACHU9J_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7515..7880
                     /locus_tag="ACHU9J_001074"
     CDS             7515..7880
                     /locus_tag="ACHU9J_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904673.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            7887..10709
                     /locus_tag="ACHU9J_001075"
     CDS             7887..10709
                     /locus_tag="ACHU9J_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1904674.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            10786..11745
                     /locus_tag="ACHU9J_001076"
     CDS             10786..11745
                     /locus_tag="ACHU9J_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1904675.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            11750..12061
                     /locus_tag="ACHU9J_001077"
     CDS             11750..12061
                     /locus_tag="ACHU9J_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1904676.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            12122..12646
                     /locus_tag="ACHU9J_001078"
     CDS             12122..12646
                     /locus_tag="ACHU9J_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904677.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            12643..12882
                     /locus_tag="ACHU9J_001079"
     CDS             12643..12882
                     /locus_tag="ACHU9J_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904678.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(13574..14620)
                     /locus_tag="ACHU9J_001080"
     CDS             complement(13574..14620)
                     /locus_tag="ACHU9J_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1904679.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(14620..16371)
                     /locus_tag="ACHU9J_001081"
     CDS             complement(14620..16371)
                     /locus_tag="ACHU9J_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1904680.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            16526..17362
                     /locus_tag="ACHU9J_001082"
     CDS             16526..17362
                     /locus_tag="ACHU9J_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1904681.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            17386..18438
                     /locus_tag="ACHU9J_001083"
     CDS             17386..18438
                     /locus_tag="ACHU9J_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFR1904682.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            18484..19284
                     /gene="gpM"
                     /locus_tag="ACHU9J_001084"
     CDS             18484..19284
                     /gene="gpM"
                     /locus_tag="ACHU9J_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR1904683.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            19386..19880
                     /locus_tag="ACHU9J_001085"
     CDS             19386..19880
                     /locus_tag="ACHU9J_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1904684.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            19880..20077
                     /locus_tag="ACHU9J_001086"
     CDS             19880..20077
                     /locus_tag="ACHU9J_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1904685.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            20071..20373
                     /locus_tag="ACHU9J_001087"
     CDS             20071..20373
                     /locus_tag="ACHU9J_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904686.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            20354..20902
                     /locus_tag="ACHU9J_001088"
     CDS             20354..20902
                     /locus_tag="ACHU9J_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1904687.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            20899..21372
                     /locus_tag="ACHU9J_001089"
     CDS             20899..21372
                     /locus_tag="ACHU9J_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904688.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            21444..21911
                     /locus_tag="ACHU9J_001090"
     CDS             21444..21911
                     /locus_tag="ACHU9J_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904689.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            21904..22539
                     /locus_tag="ACHU9J_001091"
     CDS             21904..22539
                     /locus_tag="ACHU9J_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1904690.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            22536..23117
                     /locus_tag="ACHU9J_001092"
     CDS             22536..23117
                     /locus_tag="ACHU9J_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1904691.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            23114..23464
                     /locus_tag="ACHU9J_001093"
     CDS             23114..23464
                     /locus_tag="ACHU9J_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1904692.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            23468..24364
                     /locus_tag="ACHU9J_001094"
     CDS             23468..24364
                     /locus_tag="ACHU9J_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1904693.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            24357..24887
                     /locus_tag="ACHU9J_001095"
     CDS             24357..24887
                     /locus_tag="ACHU9J_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1904694.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            24890..26860
                     /locus_tag="ACHU9J_001096"
     CDS             24890..26860
                     /locus_tag="ACHU9J_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904695.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNATNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNQASGALQKNQNGADIPGKDTFTKNIGACRAYSSWLNIGGDSQVWTTA
                     QFISWLESQGAFNHPYWMCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIR
                     VTTPSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAV
                     GWNWNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEAD
                     WSEFYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTG
                     SVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            26863..27396
                     /locus_tag="ACHU9J_001097"
     CDS             26863..27396
                     /locus_tag="ACHU9J_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1904696.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(27425..27952)
                     /locus_tag="ACHU9J_001098"
     CDS             complement(27425..27952)
                     /locus_tag="ACHU9J_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1904697.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(27954..28940)
                     /locus_tag="ACHU9J_001099"
     CDS             complement(27954..28940)
                     /locus_tag="ACHU9J_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904698.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTDSY
                     MWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGDIF
                     ANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNN
                     WQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            29045..29632
                     /locus_tag="ACHU9J_001100"
     CDS             29045..29632
                     /locus_tag="ACHU9J_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1904699.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            complement(29668..30156)
                     /locus_tag="ACHU9J_001101"
     CDS             complement(29668..30156)
                     /locus_tag="ACHU9J_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904700.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(30169..32976)
                     /locus_tag="ACHU9J_001102"
     CDS             complement(30169..32976)
                     /locus_tag="ACHU9J_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1904701.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(32963..33118)
                     /locus_tag="ACHU9J_001103"
     CDS             complement(32963..33118)
                     /locus_tag="ACHU9J_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1904702.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(33127..33492)
                     /locus_tag="ACHU9J_001104"
     CDS             complement(33127..33492)
                     /locus_tag="ACHU9J_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1904703.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            complement(33547..34059)
                     /locus_tag="ACHU9J_001105"
     CDS             complement(33547..34059)
                     /locus_tag="ACHU9J_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1904704.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            complement(34059..>34602)
                     /locus_tag="ACHU9J_001106"
     CDS             complement(34059..>34602)
                     /locus_tag="ACHU9J_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR1904705.1"
                     /translation="AYIDHEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNN
                     KEITTIIRRNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANV
                     KDVIGAIRAKLDSLVTAGKLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDME
                     LYQSFTDEFFGPAFAVLGGA"
BASE COUNT         8688 a         9441 c         8809 g         7664 t
ORIGIN      
        1 tcgtctcgcc tgccagaaat ttttctgcca atagacatta tcaagtgagg aacgcatcac
       61 tcccttgctg gtagaggcgt ggataaattg gttgttggta tcgtaaatac ctacatgcaa
      121 accattttgt ccggaacccg ttttgaaaaa gaccaggtca ccaggcagca actcgtcttt
      181 atcaatttgc gtgccgatag aggcttgttc tttggtttct cggggcagct gcaaatcgaa
      241 acgatcgcgc atcgttacaa ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc
      301 accataacga tacggcgtgc catgccagct ttgtagctgg tcgttcaaac cggcaataac
      361 ggtaatcgaa tcagaaagtc tggcatttgg cggcggtgct ttatggtggc tacacccggc
      421 cagaaacagt gctgtgatca aaataaggca gaaacgcatt ccgtacggtt cctctgtttt
      481 ttattcttgc attaatttag cgtcgtaatt acccgatttt caagatacta atgaaatcag
      541 atggtcgaaa tcagcattct gtgaccttcg atatccagac ggcgaaaatt catcccatag
      601 gcctgcgcca gatttggcgg cgtgagcacc tcttctctgc gtccactggc cagcattttt
      661 ccacctttta gcaaccacgc ccgatgcgca tgacgcaatg tgtggttgag atcgtgactg
      721 ctcatcacaa tcgccagtcc ttgctgacac agagcgctca gaattttgtc taacgcactt
      781 tgttgcgcaa catcaagact gttcatcggc tcatcaagaa gcagcaattg gcctgcggga
      841 ttggcttgtg gtgtgatttg cagcaccacc gcagcaagac gtacgcgttg ccattcaccg
      901 ccggaaagtt gattggtgct acgtccgagt ttgtcatcaa gagccagcgc ccctgcgaca
      961 tcattcagta gttcggtacg cgttttatcg tgctgatgca gtgtcagata gtgccagacc
     1021 ggcattgcaa acggcggcgt ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt
     1081 gttgcggacc atgcttccag tggttgcccc gcgaactgaa tacttccctt accgctggtc
     1141 attccggcca ttcgtgccag taaggtactc ttacccgcgc cattcggccc caccaggtgc
     1201 aggatctccc cagcccgaac ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca
     1261 tcctgtaact gcatcacaat agacattatt ttgccaacgc cagtttaatg ctttccatca
     1321 caatggggtc ttccggcgtc atatccgggg aaaaacgctg gatgaccaat ccgtccctgc
     1381 caactaaaaa tttctcaaaa ttccataaaa tatcatcagg gtacagcggt gcacgacctt
     1441 tgctgaccat acgggcatag aatccgctct cttccggcgc gactgcggtc ggcgctgcgg
     1501 caatcaattt ttgatacagc ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca
     1561 tcgggaacgt caccccccat gtggtggtac agtaagtttt aatctcttca tcgctgcccg
     1621 gttcttgttc cagaaactgg ttgcacggga atcccagcac cacaaaacct cgatcggccc
     1681 aggctttctg aatattctcc aactgctcat attgcggcgt taagccacac tttgaggcga
     1741 cattgacaat caacagcaca ttaccggcgt acttctccag cgtggtcact tcaccgtcga
     1801 tatctttcac tacggtcgtc agaatggaat cttgcatcgt ttctcctggg tgtggtcagt
     1861 aaaaatctta gcttttaatc atagaccgtc tttttgcggc taacgtcctg cttttaacaa
     1921 taaccagata aacaccggcg cacccaacgt tgcggtgacc acgccaatag gcagctctgc
     1981 ggcagctaat gccaggcgcg ctacaatatc ggccagcagc actgcgctcg cccctgccag
     2041 cgcgcagccg ggaagtaata cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg
     2101 gatcaccaga ccaataaagc cgatagcacc cgccagcgcc acactgacgc caaccatcca
     2161 gccggtcgct gccaccagca cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc
     2221 cgagatctcg ccaagtgcta acatattcat cggcctggac tgacaacaga tccacaacaa
     2281 cacggggatc aatgccagca tcagccagct ttgccgccag tctacgccgc caaaaccgcc
     2341 catcatccag tacatcagct gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt
     2401 cattagtgcg ctacagataa tccctaatgc aacgccagcc agcaataacc gactggtcga
     2461 aagatgacga cgggcgaaac gtaagagtat taaagtgatg ataagcgcgc cagcaatcgc
     2521 acacagccct agcgcccagt tggggagttg cccttgccca agcaataccg cggcgataag
     2581 ccccacgcct gcgccgttag agacgccaag tagtccaggt tctgccagag gattttcaaa
     2641 caacgcctgc attacagcgc cggatatagc cagcgccgca ccaaccagca atacagccag
     2701 cgtacgtggc aggcgaattt gccagacgaa cagttcgcca cgaggagtaa accagtcacc
     2761 tggcgaaatc cattgttcac cggcgcaaag gcttaagaga agcgccagca gcatcaaaac
     2821 tgacaggcat aataaccagc gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat
     2881 ggtatccgtt ctgctgaagt gtcatggcgt tgattttacg gtgactcttc gacagtgaaa
     2941 agaaaaaagg ccgcagagcg gccataaaca caaacaaaaa tcaataagtt aggtaattat
     3001 caaagactta cagacacaca aaaacacagc caaccacaac aaataacaga gatgtggtca
     3061 atttgtggat catcagccaa caaatttagc aacaaataca gcaaccgccg ctgtgaaaat
     3121 ggttgtgcac actggcacaa taatgccact ccaacgccac ttcgtattat catcaacctt
     3181 gtcaaaccgc cgattcatct catccataat cttgtcaaag cgtttatcca tcgcttcggt
     3241 aagctcaccc ttcaggctgt taatgtctga tttgagctgc tctttcaggt tagctgtatc
     3301 agatttaagc tcacctttca gattcgcaat atcgcattca agatcacctt tcagatgtgc
     3361 tatgtctgct ttgagttcac ctttcacctc accaacatcg gctttagttg agaggtttgt
     3421 agtgcgttca gcaagcgtcg caaggtcgtt tcttgttagc gataaatcac cttcgagccg
     3481 ctcaattcgc ctttcaagtt tatctgacat tccgcctcca ctactgccgc catcgccacc
     3541 atgataaagc acatcacgct cagaatgcga cacactacct gctgaattac cgaaaagatt
     3601 tcttgctaca ttacccatta tcaccatcct gcgctttgtc tttctccaac cacttcagca
     3661 ccgcagacgt acgataaagg ctcaggtaac cacaattcag gcaatgcagc gtgtaataag
     3721 ttttgcgcat atcaggtatg ctcttaacat cacccaaaaa agcataagat acataatgct
     3781 ttatgttctc tttaattaac tcactctgtt cctccagggg tagattgtta aaattttctg
     3841 gtttcttatt tttatcccag caaagatcca tactttctgg aacagataat cgcgacgatc
     3901 cacatgaagg gcagacactg gagaccccct tctcagacaa gaaacggaag aagatttctg
     3961 gcgtaatctc catcagccgc tctttatgtt ttttgacacg actcatcgac ttcttattga
     4021 tttcatcagc ttcttctttg catttatctt ctatatattt cgatagtaaa tagtcatata
     4081 actctttttt tgtaggtttt tttatttcca tagttacatt cctcccaagg gattaaacct
     4141 caccgcatcc tgcaagtaat ccggcgcaag atgggcataa atcatcgttg tctgaatctt
     4201 tgcgtgcccc agaattttct ggagcgtcag aatattgccg ccgttcatca tgaaatgact
     4261 ggcgaaggtg tggcgcagcg catgaacagc ctggccgtca ggaacatcag gtgcgaccgt
     4321 tttgatgaca tcgcgaacca atgaataatc cagcgtcgga aacaccagtt tcccgccccg
     4381 ttttttgatc ttttcaaaca ggctttcaga aataggaacg gtacggtttt tgctgttctt
     4441 cgtttttgaa aaagtgattc gacaatgaag aacacggcgc tgctccagtg ccgctacctc
     4501 gccccatcgc gccccggtcg acagaaggat ttcgacggcc agccgttcat cgggattttc
     4561 agccagtgca tccagcaact gaacacattc agacttactc agatatccca tttcacgctc
     4621 gttaaccttc attcctttaa ggccttgaac ggggttatcg ttaagaaaat ggccggatga
     4681 gatgagtgcg gtaaacatcg cgcttaacgc cccaatctct cgatttatgg tgctgggctg
     4741 tatcccctgc tctatcctgg acacacgtag ctcggtgagc atcgttgtat taagtttatg
     4801 cacgcacggg tcatccattg cctcactcaa gcgcagcaat ttaaggcgcg tgttatgccc
     4861 tgacttcatt agctggccgt ggtatttcca ccacaagtca ataagcactg acagcggacg
     4921 gcgatcaatg gagtttcctt tccactcatt gttatgctgt tgcgccagca cccaccgctc
     4981 atataaaact gcatccgatt tcgttttaaa ttttttgcga atgcgtttgc ctttacgccc
     5041 ctcaggacgc atgtcaagaa gataccctcc cggaattgat tttatgctca ttcgtgaagc
     5101 cccagcgtta caagaccacc atgcccccag cgttccatga ttagccgggc tgtgtgccag
     5161 tcttgcggga tttttgagaa gacgatgtgt tttctggccc atcaggggag agagaaggac
     5221 tgatctgccc agcagcctca tttgtttcgt tactcatcaa ccacgtagtg tattttttaa
     5281 attccgggtg cattgtgatt ttcagtaaaa cctccgttcc cacacctcga ataccagtct
     5341 cgtattgctt aaccgtacca accgcaatac ctattgaatc cgcgaacttt gcctgactaa
     5401 gcccctcaga ttctcgaatt gcttttagct tctttgatat ctctattgac atggtgtgca
     5461 cctgaatact atattggtta tcagttgagt accacttgac gctacaaaga gccacaaata
     5521 gcaccagatt gaataggtta tcacaccatg gcaaaagtcc ttaacacata cgaacaagcg
     5581 gattttgagc gtttggcggc gttctaccca taccgtaatg aacatgggct accggtactt
     5641 gaagaaagcc tagaggatta tgcgaaacgc acaaatcaaa gcattctcgc tgtaagaagg
     5701 caggctgata gatcggtaat tcccgttact caagaaggaa gaaacacaaa acgcaaagta
     5761 aatctcttcg caattttcct gaaaaccatc agaaacgcag agaaatacgt gcagatgaca
     5821 aaataacgag gtgtcatttt atgctgaagc aacgccgtaa tttttgtacc ggaacagaac
     5881 gccacgctaa ccgtttcact accagtgcat cacgcagcaa catccgctac agcctgagtg
     5941 atacacacgc aacgccggat ggctacccag taaaacaaat cggcgagcac gcctggctga
     6001 ttgagaaagc tggaatcgtg atccacaaat gcccacgcaa tccgtttacc ggaaaccgca
     6061 tttttgcact gagctgtggc gacaatcagt tcgggcagga tttcacatta tacgaagcac
     6121 ttcgcacggt tgatcgtctg cttcgcgggc aaagttttat taaacaggct gatttataac
     6181 aggtgcttta tgaccaaaga caatgcacaa ggtgtattta tccgttttat tgattttcgc
     6241 ggtgaactgt tattacgtgc atccgctatt gacggagtga ctccggcggg taaaaacgga
     6301 gccgacgaag ccacttacgt ttatctgaac ggcacgcgac tgcttgtgga acttccgtac
     6361 cagaccgtac gagaaatcat cagcgaagat gaaaaggcac gccaggttaa tggcgatgaa
     6421 ccctatatcg aaattatttg tatggattca gaagctgaaa tacagaaagc agattaaagg
     6481 gcgttgtgat gggcaaagaa tataaaactc tcattaacaa agcacttgag cgtttttatt
     6541 ttcgcttaag tgcatcaggc gctcatgctg aacgtgcggc ccgtgactca ttgaccagag
     6601 caatccgaag tctgtatgac gtggcttttt acgctgatga tctggatgca cttaacgaac
     6661 tttccgagct gatctgtgcc gcagaatgcg gggaacatat tgaaccgtat aagctgggaa
     6721 atatcgcatg agtatattta tttcatggct tgttctgatt atttcggtgg tctgcgccat
     6781 tgggattatg cgaattatta attcagtaaa aaagattgaa cgctttttca ctgaagaata
     6841 accgcgcaaa taagacccca ggttaaataa gaaaatgtga aaacaatccg cattcgcgga
     6901 ggtattcgca cacgccaagg aggcgtaatg gcaattaagc attttcctgt cgttcgtttc
     6961 acctccagag gacgtgaata cgaagttgac gaacgcctga ttaccacaat cgacaaacac
     7021 cgttcagaaa aggatgcaca tcatatctat ctcactgacg gcacttactt ctgcgccacc
     7081 aacgtggtgc aggtgaatct tatcagacag gtacaggagt cacgcagatg accattctgg
     7141 actacatcgc tgccaatccg ggttgtagca gtggagaaat cgccgcagca ctgaatacac
     7201 caaccacaac cattaatgcg gagctacgcc gtctctggcg cagcggttta gtcacaagaa
     7261 aagagcgcaa aacaggcggt cgcttttctt atcaggtaaa cctgatgccg tttgggtgta
     7321 gcaacccact aacccagatg ttcaaccagc tactgaggga aatcagagca tgagcacctc
     7381 caactgccgg aaaccacgtc gggcttcagc agctcatccg gcagcaaaac aaactccatt
     7441 aattcctgtt ccgggccttt cctgcacctt gcggcgggag gccttcgcac atctgtaaca
     7501 agaggattgc cgcaatgatt ctcgccaacg actttcttga atacctgctc aacacagagc
     7561 gtgatcttgc cgctcgcgtg cgtgatcgtt atgacatgta cctgaaatcc ctgcctgtac
     7621 cgcagctcgc tgacggaaag attgttattg atggtcgcta catgattgac agccacgagg
     7681 gaaattacag gctttaccgc attgaaggtg gcaccccgtc cgttattggc atttaccagc
     7741 gcccatcctc tgcaatcgtc gatgtgattg ccgacagcat ccgcatcaca catcgccatg
     7801 ccgacacaga agacaccgtg ctggaaattc agcggctggc tacagtctgc cgcgacaccc
     7861 tgaatggcat gacgaagtaa atcactatga cggcagagta catcagggac tggcaacaac
     7921 cgcgccacgc agtggggcgt gaaggaacgg ggatccccgc tcctgaatcc gcgctttcct
     7981 cctggctgga tgcccaccgg gcagagaacg agcgccgcca ggaaatggct gatgcggcgt
     8041 tctccgccac gccactgggc aacctgatta ataaaagcct ggacgcacag gaaaaacagg
     8101 acaaaaccat cacactggca ggagacgcca gaaaacaggc acgcggcgcg gtggatgaag
     8161 ccatggcctc gctgcgcctg ctgccgtcct atctgcgcga tccgcttatt cgccacctct
     8221 ccttcctgcg caaaaaacag gaagccgatc gccggaaagg caaaaagagc tggcaggcgg
     8281 aacgctatgc acgcggaacc ctgcgcaaaa tattcgaacg tctggatcgc actgacggac
     8341 actggctgac accgggttat cgctcccttg ccggacgtga acgcctggac gatttgcttt
     8401 acctgccgca actcaacaaa caccagatac agacgctggc caccatgacg gcggcgatgt
     8461 tcagcagcac cttcgaaaaa ctctgcgatg gttttggcgc gactgatggc gagctgacca
     8521 tggatgtaac gctgaaggcg tatcagatgc tggcccgcat ggcgttacac ttacacgcca
     8581 tgcctccgca ttatgacgca ctgacaacag acaaagaccg gaggcacgaa ccggacacag
     8641 aactgctgcc gggcgcaatc cttcgcctga cctgtgcgga atggtggaaa cgcaaactgt
     8701 ggctgttacg ttgcgagtgg agagaagaac aacttcgcgc cgcctgtctg gtttccagaa
     8761 aaacatcgcc ctatctgagc caggacgcgt taagcgagtt tcgcgcacag cgcgagaaaa
     8821 cacgcgattt cctgaaaagt ttcatgctgg aaaatgaaga cgggttcacg attgatctcg
     8881 agacggtgta ttacgcggga gtaagtaacc cggttcaccg taaggcagaa atgatggcca
     8941 ccatgaaggg gctggaactt ctggccgaag cccgtggcga caaagcggtg tttctgactg
     9001 tcacctgccc gtcaaaatac cacgccacaa cggagaacgg tcatccgaat cccaaatgga
     9061 acggggccac aatgcgcgac tccagcgatt acctggttaa cacgtttttt gcgacggtcc
     9121 gcaaaaaact gaaccgcgac ggcctgcgct ggtatggcat ccgcacggcg gagcctcacc
     9181 atgacggcac cgtgcactgg catatgatgg tctttgctca tccggaagaa atcgacacca
     9241 ttgtgtccca cacccgcgat attgccattc aggaagatcg tcacgagctg ggcgatgaca
     9301 taactccgcg ctttaaggcg gagtatgtag acggctcaaa aggcacgcca accagctaca
     9361 tcgccaccta catcgggaaa aacctggaca gccgcgccgt ggatggcatc gacccgaaaa
     9421 cgggcaagcc acgcgttgac cacgaaaccg gaaaatcaat ggccgagagc gtggaacgcg
     9481 ccatcggctg ggcgcgcctt caccgggtcc gccagttcca gttctttggc atcccctccc
     9541 gccaggtatg gcgtgaactg cgccgccttg ccagccagat ggcacgcaac ccggaaggcc
     9601 cgcaacggct gaaggatgac gcaatggatg cggttcttgc cgccgctgat gccggatgtt
     9661 ttgccaccta catagagaaa cagggcggcg tacttgttcc acgcaaagac tacctgattc
     9721 gcaccgccta cgacctcgcc gatgagctga acgattacgg cgaacagagc gtacagattt
     9781 acgggatctg gtcaccactc atcggggaat cctcccgtgt gtgcacgcat ccggataact
     9841 ggaagctggt aagacgcaaa ccggaagcgg aagacagcgc ccgcgaaaat ggttttgacc
     9901 ttcagggcgg ccctgccgcc ccttggactc gtggcaataa ctgtccccgt gtacaggaaa
     9961 cggacaacaa cgggacagaa cagccggaag aacggccagc accgtggccg cagctccctg
    10021 acggcgttga tgtggatgaa tggatgcgct cactgaaacg gcacgaacgc cgggcgctga
    10081 tgcgttcgct tcgtgacaaa caggcaaaaa acagcagtga tgaaatgcag agctggacac
    10141 agagccgcaa acagcagcgg cctttgcctg ataaccacga attactcgct aaagaatggc
    10201 gggagtctgc tgaatctctc ggcctgcata tcggtgaaca acagatgcag cacctgttac
    10261 ggggcggcag tctgtacgtt gacggcagca tcattgcacc gcagggattt gaaattgtac
    10321 gcaaaccgga tacccgcccg gacagccgaa tcacgcagct ctggcagcgc ctgagccgta
    10381 atcatggcgt aagcagcacg gagatccgcc ataacccggt cgccagctat ctgacacagc
    10441 tgggggcatc agaccctgaa gccgccgcac gcctggcatc cacacttcag caggaccaga
    10501 acaccatgaa aacacccgtt accgtgcttt ctgacatgct gcgcgccatc cgcgacgcag
    10561 agcacgcaca gagaatcagt gaaaccactg aacgcgcccg ccgcaaagca gacctgctgc
    10621 ggggtggcct gaccagtgga aacaaaaaac agacagaaac gggactcacg aatcccgtaa
    10681 atgagcaaaa aacgcgcagc gatatatgaa gcgcgcacaa aacaggcaaa aacgggattt
    10741 cagaatcccg taaacgatta attaatcaac ataaggaaaa gcgacatgaa aatttgtatc
    10801 gacgacggct ccaccaacat caagctggca tggactgaga acggcgaacg ccgcaacgcc
    10861 atcagcccga acagcttcaa gtcggaatgg tctgcgccgt tcggtggcat gcagcccgcg
    10921 aactacatgc ttgatggcgt gcgctatggt tttgatccgg tcagcgatcg ctttgtccag
    10981 acaaccgaca cgcaatacca gtacagcgat gtaaatgtca ttgccattca tcacgcgctg
    11041 gtcaaatcag gcatcacgcc acaggaggtg gatgtggttg tcaccctgcc actgagcgag
    11101 tatttcgaca caaacgcaca gccggacatg gccaacatca accgcaaaaa agcgaacgtt
    11161 atgcgcccgg tggagtacca gaacggcgaa gcattcacta tccgtaacgt gcgggttatg
    11221 cctgaatcca ttccggctgg ctttaaggca ctggctgaca tgagtccgtt tgaatccctg
    11281 ctgattgtgg atttgggcgg aaccacgctg gatgtggcaa aggttcaggg gcaactggca
    11341 ggtatcagcc aggtgttttg cgatccacac gtaggcgttt ctctgatggc cgatgccgta
    11401 ctgtcggtga tggccactaa cggtatgcgc accagtcacc acatcgccaa taccattatc
    11461 gaacatcgcc atgatgaagc ctggctgcgc cagcacatcc acaatgacgc gcattacgcc
    11521 agcctgatgg cggttattcg tgaaaaggaa gaaacactga aacaacgcgt gatccgcgcg
    11581 ctggcgggtt tttcgggtta cgggcgggtg atggttgtcg gtggaggggc ggagattgtg
    11641 gcacccgcta tccgcgaagc ctgcggagtt aatgcgactt tcatcgcgga cggggtgcca
    11701 cagtttgctc tggttaatgg gctgtacgca atggacaagg agtaaaccaa tgacgacacc
    11761 aaccagacgg ataagtttct atctgaagcc tgcggccgtc aagaacgaaa gcgaagcatg
    11821 cgcctggctg ggcagcctta caccagaagc ccgcaaaagc ggccaacgcg tggcttttct
    11881 ggccgggctg gcacttctga aaatgaatcc agcagaggct taccgactgg ctgcatgggc
    11941 tgacgatgag gcgttatcgg tgacacaaac caggacagaa cgccccgtgc cacagccagt
    12001 accaaccgca cagataacca gtcagatagc agggaatatc cgggcattat ttcctgaatg
    12061 acgaatttga cggcttttaa ttttatcggg gtgcactgtc gccccgcacg aggaaaacac
    12121 gatgaaaaaa tacgaataca tgctaatgaa agaagcactg cgggctggtc tggctccctg
    12181 tcctgtatcc gtagaagctg ttgttgggaa cacgattttc cgaacgctgc gaaatcgcgt
    12241 atccgtgaag atggttcacc aaccacccaa atctaccagc atgatctatc tgccgctgcc
    12301 cgaggctatc aaacttatgc gttcgctgaa atttgcgatt tggatgcaga cacaaatcaa
    12361 caaagcggtg caaaacacct tcgttaagag actaataccg gagcttgctg ccagatgtgc
    12421 aaaatgtacc agacccgcac ttcaatttga tctgtcattg attcaacaag ctacggaggc
    12481 agaactgtac cgcctgtcac ctatccgtga tgctcacacg gaagcatcag aaatgttccg
    12541 gctcatgttt caatttaatt ttgccgttcg acgcacacgc gttcaggtca atcgcatgat
    12601 gcaggagcag gaagagttaa cacagtacaa agggggcagc cagtgagtaa ttcagcacga
    12661 ctacagcttg gtttttcacc gctatcaaaa accatcgtat tggcaaaaat gcgtgactta
    12721 ggagacggaa caaaacgccg cgtaggaaat gatcgcggtc gcgatgtaac caacgaggcc
    12781 gctcaactcg tttggcatct agtcatggca gaaggcggtg aaattaattg ggagctggat
    12841 gatggttctc gtatgatttt gagggccgag aagagtaaat agctcaaggg ggattaatgc
    12901 taaatgatga tgaagaagag ctgcttatgc aggaatggtc gctcggtgat tatgataatg
    12961 gtgaaaatgg gtgcccgcat tgtggccgtt atcgtttatg tatctgccag aatggaaaac
    13021 acagatgtga aaagtgcaat tggtctccgg aacttaatga ctacgtgccg atcgagtggt
    13081 agaggtaagt cacactaggt gttttttagt tgattttata aatgcacaat agtgcacaaa
    13141 tttgcacaat ttttttgaac gactttttgc ccttccggcc cgcgtggcgg ctggatccgt
    13201 caaggatccg tgcatgcaca aaaaaacgcg ttttttctgc gcgcaggtga cgggggaaca
    13261 gcccgcgttt cagggggtaa atagcattcc ctgaacgatg tcgcagagat acaacagaat
    13321 ggctgtattt ctcacgctga gcgtgaaaaa gacgtgaggg cttttgattt gatggggtga
    13381 aaggtaaggc cgtcaaaatc gcactgagac ggcgagaata tgcagtcaac gaggtgggat
    13441 tgcgtaagag tctgactgtc gatgatggca atcagcagga aagcgtcgtg aaattatctg
    13501 actgatacag gagctggaga gtcggggcat aaatttttta tgccccggcg aagcagcaga
    13561 caagcgaagc gcgtcaggat gtgggctggg tgtctaacag tgcgtaaggg ttaaagcgga
    13621 tcacctcttc gccaagccag tcattgatgt gcttcatggc ctccatgacg ggcatcagct
    13681 cgttaattgc gtaaacccgc gcggccttct ccacatcacc aaacgcactt ttttcgcccg
    13741 gcatcgcccc catcagttgc ggcggaacgc ggtgcgcagc cagcacatca tcacgggatg
    13801 ccgccttaac attcatgaac tcatcctttg cggtgatctg ctggaacggc aaaatttgca
    13861 ccccctcttt gcccccgttg ggcgcatgaa tgagcacgtt tttaaacgca ccaccaccac
    13921 gtgccccctg tagcgtttct ttcagggagt ccatgctttc gcggtttacc tgcgctgcac
    13981 cgatgtagat gatgcacccg gcgtgggatc cgttgtcgta gtacagtttt ctgaacatgt
    14041 ccgccgaatg agaaaggctg gccgagagta atgcgccaag atattccggc atgccgtaga
    14101 tttcctggtt aatatccgga ttcatcaggt ggcacacttt gccaggacga aactgaaacg
    14161 catccttgcc atcctgcaca taccaccatg attcaagatc gcttccgcgt cgcatgtatt
    14221 tcgccagggc gtgccgtaat ttaagcggtt cgccgagcat attgctccga agctcaagga
    14281 atgcgttacc gaacacaaac cagtccagcg ccagcgccga gaaatcctgc cgggaaagca
    14341 gcgggtgcgg aatatagcag ccgagcaata cattgcgctt aaagtaaagc gcagactgat
    14401 gccaggacgt ttgccgggca gctcttgcca gaccgtacca gtccaccggg gtttcatacc
    14461 accgcccgtt atcagcacag tacatattgt ccagcaggtc atgcccggtc aggcggtaag
    14521 gaccatcaaa tgtgaatgca ctgagcgacg attctttcct gagcgcatca gcgagatcaa
    14581 tgcgtgaact catgcgcact tttttatttt ttctgctcat cagaactcca taaccgtgaa
    14641 acgctcgttt tctccttcgc cgccaatcgg ttcgttaatg acagcaagca tggttgccca
    14701 cgcaaggtcg ccgtggctga tccccctcgc gcggtccgtt tcgtaagtga taaaaccgcc
    14761 cggtgttttc accttacgca cggcgttaaa ggccgcaacc agctcgcgtt cggcgcgatc
    14821 gtattcccac cgcccggcac gcattatttg cagcattttc agtaccagcg accgttttga
    14881 tgacagcgtg aaggtgtaag ggatggcggc gggaaaaaac cgtttcacta tctgatagac
    14941 ggcatccccg tgcccgcccg tcgcatcaat gccgatgtgt tccacgttgt agcgatacgt
    15001 gaactcttca ataactctgg cctgctcttc aaattccagc ccctgaacgc gtcgcgtctc
    15061 caccgttctg aaacgaccac caggaacagc cggaggaacc accacggaca cagcgccgct
    15121 gtcgccgtta ccactgctgc cgtttgcgtc atacccaatc cataccggac gattccccat
    15181 cgggcggggg gcaaagggtt tccagtcttt ccagtcgtcg tatccgtcaa caccgcagcc
    15241 aatcaggata ttcaggttaa atgccgattc cccttcgcgg acaaactcac acatatagag
    15301 attgcggaac tcgtcttcgg tgttttcatc acgaatttca tcaatatcgg tgtgtttcca
    15361 gccgtgatta accacatctt ccagcgtgac aatttgccgc cacgtccggt cagggcagat
    15421 aagcccgtta tgcagcgttt tccagtccac agaaaaacgc tggcgtttat gcgcggcctt
    15481 tttctcgttc cagcggtcgc cgttccagta ggcgtatgcc tcgtgcgttt cggtggatgg
    15541 cgtggagaag caggtgcgcc gcagtccgct gagggttgcc atagcgccag ccaccttgcg
    15601 cagttcagca aagcgactga cccagaaaaa ttcatcaaaa taaaaattgc ccgtgtaaga
    15661 ttgcgccgtc gcagcagaag tgccgagaaa atgcatctct gcgccgttgg agaggatgat
    15721 tttatcgccc cctttcagct ccacatcgac ttcagccgcg gccttctgaa taatgctttt
    15781 aaactggaac gcctgacgac gcgacgcaga caaaaaaatc tggttacgct ggtaaggttg
    15841 tgccacatcg tcacgcagcg ccatcagcag tgcttcctgt gcaaaatacc aggtcgcccc
    15901 aatctgtcgg gatttcagga tcatcctgtt acgtatcccg gcttccctgc aaagcgtcag
    15961 ggagtcaaac cagccccgct gatgccactc cagcctgctg atgatttttt cccgcagtgc
    16021 ggcaatctgt tccggcgtga aatgattttt gagttttttc gcccggcctt tctttcctgt
    16081 ggccgtcgca tccggctggc catcatgcag ttttttaagc tgccgggtca gcaggtctat
    16141 ttccttaaag tcaccacctg ttttattctg tttttcagta agctggatga ggcgcgcatc
    16201 gatggactgc gtgacacgct gcacgggtgg cgtttcatcc cactggtcgc gttttttcca
    16261 cgcataaatc gtgttcgggt ttattcccat cagacgtgat atttctgcgg gcggataacc
    16321 ctgccagtaa agttgccgcg cacgctggcg cacaaaagcg tcctgaatca ttgctccccc
    16381 tgagtaatta caggaagatt acccgcgcgc gaaaccgttc tccttaaccc cctgttctgg
    16441 ccgttttctt acaacaaaag ccctttgtat cagcctgtta cgctttgcca tcatgactga
    16501 agaaccagtc agaggggcaa aaactatggc taatgaaaaa aagacatccc gcaaaaagtt
    16561 tcgcgtggct gtctccggat caactgttga tggccgtgaa atcagtccgg tgcatctgcg
    16621 tgaagccgcc gagaacttca acccggatgt ttacgctgcc cgcgtgaacg ttgagcacta
    16681 tctctcgcca tgcccgtcaa gcgaattttc cgcaatgggc gatgtcaccg cactgagtac
    16741 ggaagacatt acggaaggtc cgctggccgg acgtactgcg ctgtatgcag aaatcgaacc
    16801 gaccgagcgc atgaagcagc ttgtcgcgga cggcaagaaa atctattcca gtatcgaact
    16861 gcacccgcag ttctccgtta acgggcgcgc ctatctggtc gggctggcga tgaccgacac
    16921 cccggcaagc ctgggcactg agcgcctgaa attcacggca cagcaacgtc aggcggtgat
    16981 gacgttcaac agtatccagg gtgaagcacc gcttatctcc gaagccatcg agtctgaaat
    17041 catcgaaatg gcagaacaac gccaggaaga aggcacccag tggtttaacc gcgtaatggg
    17101 gattattggt cgtggccgca aagcggatga cgccagtttc tcccgtattc aggaagcggt
    17161 ggaaagcgtc gcaacgtcac aggccgacat tatcgaccgt tttaatgcgc tggaaacacg
    17221 ccatcagcag gacagccaga aaatcacttt actgaccacg gagctgacag cactgaagga
    17281 aaaactgcgc acgcaggacg gcgatccgca gaaccggttc accgcaacgg gcgcagcctc
    17341 cgaccagctg gctgacttct gataagacaa aggagcaaat tttttatgaa tctggtgatg
    17401 tcagatatta cccgcaacaa gctgggttgc tatatggcgc agcaggcgtc gcttaacaat
    17461 atcccggttt ctgcactggt atcgcgattt accgtggaac ctgcggtgca gcaacgtttt
    17521 gaaaacgcct caaaggatag caccgaactt acaaaaaaaa ttaacgtgat cggcgtgacc
    17581 gaccagaaag gcgaaaaaat cctcctggac accacaggac cgattgcacg cacaaatacc
    17641 agttatgacg gaacaaaacg ccgtaacccg aataacgtgg ttgatctgaa aaaccgcaaa
    17701 taccagtgcg aacaggtgaa ctacgacacg tttatttcgt atccgcagct tgatgcctgg
    17761 tcggcacaca gcgattttca gtcccgcatc agcgcacaga ttgcccgaca ggtggcactt
    17821 gaccgcatca tgatcggttt caacggcaca tctcacgcgg atgagtccaa cttcagcacc
    17881 aacaagctgc ttcaggacgt taacgtgggg tggctggagc acatcagaac caatgccagc
    17941 gagcgcgtaa tgaatgacgt gacgctgact tcccgcaaca tggacaacac cgtggcgcac
    18001 gcgggtaaat acgcgaaccc tgatgcactg gtacaggacg cgcgctcatc tctgctggat
    18061 gaatggcaca aggaagctga cgacctcgtg gtgattatgg ggcgcaacct gtttaactcg
    18121 ctgcgtctgc cagtgctgaa cagcatcagc ggccagaatc ccaatgcgga attacttgcc
    18181 gggcagctca tcctgtcatc gcgcaccatt ggcgggctgg gtgtgttcct tgcaccgttc
    18241 ttcccggatg caacgatgct gatcacctca ttcaacaacc tgtcgattta ctggcagaaa
    18301 ggttcaatgc gtcgtctgat gaaagacgaa ccggaataca accgcatcgc cacctaccag
    18361 tccatcaatg acgcttatgt cgttgaagac tatggcaagt gcgcgatggt cactggcctg
    18421 aagttcgccg acagctaatc aactcacggc gggcatcatg cccgcctgta acggagagaa
    18481 aaaatgatta ctcctgcaca gcaacactgg cagaacgtga tggcacagcg cgcaggccgg
    18541 gcgaatgaag gtgtggacca cgccgcgcgt accgcgcatg aagaggtgct gtatcgtctg
    18601 cgtctggcac aggcccggct taagggcgta caggccagaa gcgcgaaagc cgacatcaaa
    18661 aaagcgttgt tgccggactt ttccggctgg attgagggaa cgctggaggc tgacggcggg
    18721 caacaggacg aagtgattgc cacgctgatg gtgtgggcga ttgactgcgg cgatcttccg
    18781 cttgcgctgc gtattggtgc gtatgcggtc cgtcacaacc tcatcatgcc ggataacttt
    18841 ggccgtactg ctgccacagt gctgaccgaa gaaatctgca atccggtact gacgcaggcc
    18901 gggacggatg ccgacgcgga tttgtccgtc tttatcgaac cactggacac actttgggaa
    18961 attgtcgcca accaggatat gccggacgaa gtgcgcgcca aattatgcaa ggcgtgcgcc
    19021 tttgcccgcc gtggcctgag cgatgcggac agcatggcct catcactgaa gctgctgcgc
    19081 gaagcgatgc atctgaacca gaacgcaggt gtgaaacgcg agattgcaac cctttcccgc
    19141 gccctgaaaa aagccgattc cgcagccgca ccagaagacg ccagcgcaca gcaggcgcag
    19201 gacgaaagca gcgaaagtaa aaagacaacg cggaagcctg caacacgaaa aaccaccgcg
    19261 acgcagaagg cgaagcgcgg ttaacgactg accccgtcag cgggcggcgt gcgcggtgtt
    19321 ccggtttggc tccgtgaccg tttacaccgc gcacccaccg cccgattttt tcaggagtga
    19381 accccatgag tatggttgcc agaactgaac ccggacccgc agaggacgac atcaccgaca
    19441 ccgaagatgg cgatacccgt atttcagcgg gtgcattctg gccggatatt gtgctgcgcg
    19501 agctgcgtct ggcggtacga ctgccgggcc gcgtgaccac ctcccgcctg ctgcataccg
    19561 ccaccggggc cgtggcacac gttacccgcg agctggaagc atggcagcag gaacagcagg
    19621 cggctggcca tcagacgctg gccgatgttc cggcacctgt aattaacgga gaaagcgtca
    19681 atctctggca ctggcgcaat gctgtttata ccgccacacg cgccctgatt ctggagcgtt
    19741 accgcgatgc ggacacaacg gacaagggcg accgccgggc ggacgcactg gatatacaga
    19801 catcggattt gtggcgcgat gtgagctggg ccatctctga cattctgtgc cgcccgcgaa
    19861 tctttgcgga gttgtgctga tgaaagtgaa gacactggaa ggcgacaccg tggattcgct
    19921 ctgtttccgg tactacggca cgacgcaggg cgtcaccgaa aaggtgctgg atgccaaccc
    19981 cggactctgc cgacaggtat ttctggaagc cggacaggag gtggaaatgc cggagccgga
    20041 gaagaaaaaa caacgagaga tgatacagct atggtaacag gtttttttca gcgggtgagt
    20101 gactggatca ccttctgggc atcagtcaca gccaccacaa taggcgtgat gaccatcagt
    20161 gaaaaaatcg cccttgccgg gttgttactc ggttctctgt ctgcattaca tgcctggcga
    20221 catcgccgcc tgatggaaac cgccatgcag cgtcgcaatg aactcatcgc cagtatcctg
    20281 gcgcagtcag acgcacggcc acttcacgac agtgagcgtc aggctctgaa cattctgcgg
    20341 gataacgata cacatgataa agctgcccgt taaacgttac gccgtagccg ccattgtggc
    20401 gctggggttc tccattgccc ctcacaaact ccggaccagc ccggaagcgc aaatccgcat
    20461 tgccacatgg gaggactgtc gcgcaacgcc ttaccgggat attgccgggg taatgaccgt
    20521 ggggtgcggc tcgaccgggc atgtggaaaa tcgtctgtac accgaaacgg aagtcgccgg
    20581 gcgctgggtt aatgatatgc aacatgcaga aaactgcata aatcagaact tcagcggcaa
    20641 cgccatgcca cagtccgcgt ttgaggcaat gacagatgcc gcctttaatc ttggatgcag
    20701 aaatcttatg tggttcaggg ataagaaccg taactaccac cgcaccacta tctggaaaca
    20761 tgcacaggcg cacaactggc ctgacatgtg caacagactg actgacttcg tgaacagcgg
    20821 cggcgaacgc tcacagggac tggttaaccg tcgtactgat tttcgtgcat ggtgcttacg
    20881 tgacaccgga ggccagtcat gaaagtgcgt tacatcctga tgatggcact gaccgtcctc
    20941 acgctgctga ccataacact ctggcagaca ttcagactgg ggcaggaaga cggacacaac
    21001 cgttatacac aggcgcaact ggacgattac agccggaccc tgaaagaggc cggcaccatc
    21061 atcagcaccg catccggtgt actgcgtgac gtcatgacag cgcgccagaa ccgacaacag
    21121 gaaggggaaa tccgacgtga acagctacgc aacgatatca cgacagatgc atgtgcccgc
    21181 gctgttcctg acgctcgctt tactgagcgg ttgcaccagc acgcagaacg cgtccgcgcc
    21241 gccgccgtcg ttccgtttca tcccggaagg gctgaccgcc cccgtacttc ccccgccgtt
    21301 tccggtgacg ccatcatggg gaaacatcgg gatatggagc gacagcctca tggacgcact
    21361 ggaaacctgt aacgccgata agcgggccat tgaattactg gaacagcgca ggctgcaacg
    21421 actgaacaac gaggacaaca accatgctga aaactgattc cctgcgtgaa gccatgaccc
    21481 gttcatgccg gtggtgtcat gccaacccgg aaaaattcac cattttcgtg gagagcggca
    21541 acattgaaac gaccggagaa accccatcgt ttgtttaccg ctatcagata gtgatgtttg
    21601 tcatggatta cgccggagag ctggacgacc tcacgctgcc gctgctggcg tggttatccg
    21661 aaaatcagcc acagttgttg ctcaatccgg agcgtaatca ggacatcaaa ttttccgccg
    21721 ttatcaatga cgatgacagc gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc
    21781 gcatcacgcg cagcagtcag ggcacaccgc aggcagaaca cctgccggag ccaaaacccc
    21841 gcctgccctc ttccgaaggc gactggtcgc atgtattcca ggatgtgacg tggggggaaa
    21901 gcgatggata aggcattcac ccgcgtggat gaaacctttg aggctatccg cgacagcctg
    21961 aatcagcagg ccattaataa catcgccaga aagctggcac aggatttacg ccgcgcccag
    22021 caggcgcgta tccggtcaca gaaagcgccg gacgggaccg catggacacc acgcagacgc
    22081 cgcgtaaccc ggatacagga gcgcattcgc tttatctgga ataacgaagc acgcacgctg
    22141 aaaaactggc atcacgacac ggggaaatac gggcgaacca tcaccgggtg ggatgaggat
    22201 aaaaacaata tccgcacgtt ttaccgggat gacatcgacc gttttctgga aatacgcacc
    22261 cggcgcatca accaggacag caccaggcgc gtctccatgt tcgtaaaact gcgcaccgcc
    22321 cgctacctga aagcccgtgc agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc
    22381 gcacgtattg cacgcgttca tcagttcggt gagcgcgatc aggttgcgcc gggcattttc
    22441 accgattacc cggtacgtga gctgctgggt atcagccagg cagatgagcg cctgatttat
    22501 aacacggtgc tgggccggat tgcggaggct gtacgatgag cgcagaactc atgcgactgc
    22561 tgagcaatat catccgcacc gggatcatct ctgaagttga tgaggaatcc tggtgcgtgc
    22621 gcgttcgcag cggcgaactg gaaacaggct ggctgcgctg gaacaccacg cgcgcgggag
    22681 acttcaacgt gtggtttccg ccatcaccag gcgaacaggt ggcaattgcc tgcatcggcg
    22741 gcaacccgga aaccgccatg ataatcggca gcctgtggag tgatgccatt ccggcacccg
    22801 gcaaaagcct gaaagaaatc gtggtcagcg cgccggacgg cgcggtgttc cgctacgacg
    22861 cggacgcagg cgcactgagc gcaagcggca tgaaaacggc cactttgcag gcatccatca
    22921 gcgtgacact gaacacgcct gtcgtggaat gcacagacct tctgagaaca gcgacgcttg
    22981 acgtcacaaa agggggaacg atgagcggca atatcacgca cagcggcggc gatttcacct
    23041 caaacggcat cacagtgcat acgcataagc acggtggcgt taaaggtggc agcgattcga
    23101 caggaggccc gcagtgacaa cccgctacac aggaatgaac ccggacggga cgggaaacct
    23161 gaacgatatg gcgcacctga aacagtcagt cagggacatc ctgaccaccc cgctggcaag
    23221 ccgggttatg cgacgggaat atggcagcct tgtgcctgat ttgattgacg aacccatgaa
    23281 taacaccacg cgtctgcaat gcatgagtgc taccgtgatt gcactgacac gatgggaacc
    23341 acgcattgcc ctgaacgcca tcgacgttgt ctggaaggca ggaggccacg ccggggtgac
    23401 gctgtcgggc actgtcatgc agaccatgca gaatgttgaa ttaaccatca cgctgaggga
    23461 gtaaatcatg cctgccgttg acctttccca gttaccggaa cccgccatca tcgcggagcc
    23521 tgactttgag gcaattctgg ctgacacaaa ggccatgatg attgcagctt atcccgccga
    23581 acagcgtgaa gtcgtctccg ccgcactgga actggaatcg gaacccctga acgttatcgc
    23641 tcaaaccatg tcgtttcgtg aaatgctgtt acgccagcgg gtcaatgagg ccgcacgcgc
    23701 ctgcatgttg agccacagcg ccggaacaga cctggacaac ctcgcgggca atatgaacac
    23761 aaagcgcctg gttatcactc cggcaacgga taccaccgac gtggtgatgg agaacgacac
    23821 ctcgctgaga ctgcgggcgc agcgggcgta tgacggcctg agtgttgcag gcccgtcagg
    23881 cgcatacgag tattttgccc gcagcgccag cggtctggtg cgcgatgcgc gggccatcag
    23941 tccgtctccg gcaaatgtga cggtttccat cctgtccact gagggcgacg gcacagcaac
    24001 ggaggcgttg cttaataccg ttcgcgccgt tctgaatgca gaggataccc gcccggtggc
    24061 cgaccgcctg accgtacaga gtgccagaat cgtgacatgg cggctgaatg caaaactgta
    24121 cttttacccc ggtccggaat ccgaacctat tctggctgcg gctgaatcgt cgttcaggaa
    24181 gtggctggct gagcaggggc ttatcggtca ggacgtggcg ttgtccgcca ttgctgccgc
    24241 actgcatgtg cacggtgtgc aacgcgtgga gataatcgaa cccacacaga atatggccat
    24301 cagcgacata caggcggcgc gctgtgagtc attcaccatc agcgaaggtg ggcgtaatga
    24361 gtaattcact gttaccacca tcagccagca atttcatgcg ttgtgccgaa gccgtcggaa
    24421 cacgcattac agacattccg gcagacctca acacgctgtg gtcgccggac acctgcccgg
    24481 tgcatctgct gccttatctc gcctgggcgt tttccgttga ccgctgggat cgcaactggc
    24541 cggaagagac aaagcgacag gtgattcgtg atgcatggct gatacaccga cacaaaggaa
    24601 ccatcagcgc cctgcgacgg gccattgagc cgctgggata cctcattcgc gtgtctgagt
    24661 ggtgggagtt caacggcgaa cccggaacat tcaagattga tgttggcacg ctggacagtg
    24721 gcgtgacgga ggaaatgtat ctggaaatgg agcggttgat tgctgatgcc cgcccggtca
    24781 gccgccacat gacagggctg aatatcattc aggagattca gggagatatt ttcgcggcag
    24841 cagcaactta cgacggtgaa gtcattacca tttatccgga cgattaagca tgagtaccac
    24901 aacacgaaaa tttaaaacca ttatcaccga tacgggtgca aaaaaattag cgcaggcaac
    24961 cgcgccagat gggaaacctg tccgcctgac acatatggcc gtgggtgacg gcggcggtac
    25021 tttacccacg ccagacagta agcagacccg tcttgtgcat gaggtgtggc gacataccgt
    25081 taatcgcgtc accctggacg caacacatca gaaccacatt attgcggagc tggttattcc
    25141 tcctgaaacg ggcggattct ggatccggga aattggtgtg tttgatgagc acggcgattt
    25201 gatcgcggta ggcaatactg ccgaaagtta caagccaacc gttgccgaag gatccggacg
    25261 tgcacaaaca tttcgcacca ttctgaccgt atccagcact gccactgtgg cgcttaccgt
    25321 ggataacacc atggtgatgg ccacagtgaa ttacgtggat gacaaactga aagagcatga
    25381 acagtcacga cgtcacccgg acgcctcgct gaccgcaaaa ggccttgtcc agctcagcaa
    25441 cgccaccaac agcacgtctg aaacgctggc agcgacacca aaagccgtta aggcagtaat
    25501 ggatgaaacg aacaagaaag ccccattaaa cagcccggca ctgaccggaa cgccaacaac
    25561 accaactgcg cgacagggaa cgaataatac ccaaatcgca agcacggctt tcgttatggc
    25621 tgcgattgcc gcacttgtag actcttcgcc tgatgcactg aacacactga acgagctggc
    25681 ggcggcgctg ggcaacgacc cgaattttgc gaccaccatg actaacgcgc ttgcgggtaa
    25741 gcaaccgaaa gatgccactc tgacggcgct ggcggggctt gctactgcgg cagacaggtt
    25801 tccgtatttt acggggaatg atgtcgccag cctggcaacc ctgacaaaag tcgggcggga
    25861 tattcttgcg aaatcgaccg ttgccgccgt tatcgaatac ctcggtttac aggaaacggt
    25921 aaatcaggct tctggcgcat tacagaaaaa ccagaacggc gcagatattc cggggaaaga
    25981 taccttcacc aaaaatattg gtgcctgccg cgcatatagc tcatggctga atattggtgg
    26041 cgatagtcag gtctggacaa ccgcgcaatt tatttcgtgg ctggagagtc agggagcatt
    26101 taaccatcct tactggatgt gcaaaggctc atgggcttat gcaaataata aggtcattac
    26161 agatacaggt tgcggaaata tttgtcttgc aggtgctgtg gtggaagtta ttggcactcg
    26221 cggcgcaatg accatacgcg ttaccacgcc gagcacgtcc agcggtggcg gaattactaa
    26281 cgctcaattc acttatatta atcatggtga tgcttacgct cctggctggc gacgagacta
    26341 caacacgaaa aatcaacaac ctgcatttgc tttagggcaa acaggaagca ctgtcggaaa
    26401 tgataaagct gttggctgga actggaatag cggggtctat aacgcaaaca ttggtggcgc
    26461 atcgacatta atcctccact tcaatatgaa tacggggagc tgccctgctg tacagttccg
    26521 cgtgaattac aggaacggcg gtatttttta tcgttcagcg cgtgatggtt atggctttga
    26581 agctgactgg tcagagtttt acaccacaac ccgcaaaccc tctgcgggag atgttggtgc
    26641 atatacgcag gcagaatgta actcaaggtt tattacaggt attcgccttg gtggtctgtt
    26701 atctgttcag acatggaatg gtcccggatg gtctgacagg tcaggttatg ttgttaccgg
    26761 ttccgttaac tcaaacagag atgaactaat tgatacaact caggcaaggc caattcagta
    26821 ttgcattaat gggacgtggt ataacgcggg gagtatttaa cgatgatgca cttaaaaaac
    26881 atttctgctg gtaatcctga aacaaaagag caataccagc taacgaaaca atttaacatc
    26941 aaatggcttt atacagagga tgggaaaaac tggtatgagg aacaaaagaa ctttcagcct
    27001 gatacgttga aaatggtcta tgaccacaac ggcgttatta tttgtattga aaaggatgtt
    27061 tcagcaatta atccggaagg cgcaagcgtc gttgaggttc ctgatattac agcaaatcgc
    27121 agggctgata tttcaggtaa atggatgttc aaagatggcg tagtgataaa gcgaacttat
    27181 accggggaag agcagaggca acaagcggaa aatgaaaagc aaagcctgct acagctcgtc
    27241 agggataaaa cccagctatg ggactcacag ctacggctgg gcattatttc cgacgagaat
    27301 aaacaaaaat taaccgagtg gatgctctat gcgcagaagg tcgaatccac agacacctcc
    27361 agcctgccag taacgtttcc cgaacaacca gaatgaaaca aggcccgata tcgggcctta
    27421 atttttactc aggcttttgt ggccattctg gctttgccgt atccacacgg ctgaccagaa
    27481 cactgtagcg ttcccatgct tccagtcgtg tgcgttcctc gtccgtcgcc atattcagcc
    27541 tgacagcgcg ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt
    27601 gtgattcggc ctgttgttgc tgttcgtcag ccgtataaat ccgtttaacc acagctccgt
    27661 ccttaaacat ccacttaccg gaatcatcag cacgacgatt agctgtaata tcaggaacct
    27721 caacgacgct ataaccttca gggttaagcg tggaggcatc tttggtgatg gcgacaataa
    27781 tattatttgc atcgtaaaca atctttattg tgtctggctg aaagttcttc acttcctcat
    27841 accagttttt tccgtcctca gagtaaagcc agatgactcc gtgcttcttt gttaactcat
    27901 actgctccac tgttttagca ttacctgctt ttatgttctt taagtgcatc atattaaata
    27961 ctcgccacat tataccaagt gccatttata tacttttgca ctggcctgta atgaacgcct
    28021 gcaatattat cggcagagtt ttttccggta tcctgtacgt taatccccgt cagtacatag
    28081 cctgacgggc actgaaaatt ccatgtttgc cagttgttca ctccataata ttgctgtgaa
    28141 ccaagtcgaa catctttcac atatctggaa tcaaaattgc catagttgcc gggaataact
    28201 tgcgagccgc aaagccagtt cccgttatta tccatgtacg cctgaccatc ggtgccattg
    28261 gctgtccttg agttattaat catgtagatg ccaaattgct tatttcccag accgccaatc
    28321 ataaatttgc ggtcggcatg gtcctgacgg agcaaagcct gagcaccatc agtggatacc
    28381 gcattacgtc ccaaaataac attctggtca cgcatatgaa tccacatgcc ggtactactg
    28441 ttaattgcaa aacggtttgc aaatatatcc cctgtaacat caagaccatg ccccatgctt
    28501 atccggccag ttctgagatt aagcgtaaag gggcgtagtg gccctatatt accattttcc
    28561 ccttcattct ctcgtgtagg gatgatatgc aggcattctt cagaacgacg aaaaatggca
    28621 ccaaaagatg aattaaatat cctcagtgca ttgactgtcg atattttcac ttcactgctg
    28681 aaaagggctt caacaagaac agacaaagca ttccatttaa gattcatcag gtctttaggc
    28741 cgggtgccaa tgatgcggtg tctccatttg aaatattcat tgccgttgtt gcctgtttca
    28801 aaccacatgt aggaatcggt gtctgcatcc gaattatttt taaacccaat ctttgcccag
    28861 tcagtatttc gaatccaggc aaggattgag tcgttttcaa aagtaagtcc accggacaac
    28921 gtatcgccat ttttttgcac ggcgttacca gccttgttta ccgtttcctg taaaccgagg
    28981 ttttagataa tggccgtttc cggcctgcat ggcatgattt gcgcttttgg acgggagatt
    29041 cagcgtgctg attggctatg taagggtatc aacaaatgac cagaatacag acctgcaacg
    29101 aaacgctctt gtttgtgcag gatgtgaaca aatatttgaa gataaattaa gcgggacaaa
    29161 gacagaccga ccgggattaa aacgcgcttt aaagcgcctt caaaaaggtg acacgctggt
    29221 tgtctggaaa ctggatcgcc tcgggcgaag catgaaacat ctgatttctc tcgtcgggga
    29281 actacgggag cgaggaatta attttcgcag tctgaccgac agcatagata catcttctcc
    29341 aatggggcgt tttttcttcc acgtgatggg tgccctggct gaaatggaac gtgaattaat
    29401 tgttgaacgt acactggccg gactggcggc agcacgcgca cgggggcgca caggcggacg
    29461 tcgaccgaag ctgacaaaag aacagcatga gcaaatagca aggctgatca aaaacgggca
    29521 cgacagaaaa caactggcaa taatttacgg cattggtata tcgacgattt atcgttacca
    29581 ccccgcagga gaatcaatcg gaacaataga gaagagtcag gaaacaaaat aaccgctaat
    29641 ctgaccatta gcggtttttg tgttaaatca gaacaaccct ttaactgaac tggccgcgct
    29701 gttaagggat gatgttacct tatctttgaa gccggacagc atatcgctga acgatgagga
    29761 ttgcaggcgc tcccgcaaat cctcatcaca gcgttcaaga gtcagtgaaa attctatctt
    29821 tttcgcctta ccgtagcgat caaactcgga gcgggtcgta ttcgtttcgg tcaggacata
    29881 catgccgtaa atctgcccga cgccatcaat cagaggccag ggtcgtcctg tatacgcctg
    29941 cgtggtcagc agcgacagcg acacttcgcc acctgtaatt tcaggataaa gcacaccaga
    30001 aagaacgatg cgctcatcac ctgcaccgat atactgccag cttgccgaac ggttaacgcg
    30061 ttcatttttc acatgccgcc agcttttgtt ttgctgtaac tgctgatgcg gcaatgtacg
    30121 cagctcaaaa acaaacatgc cgtagatcat catcatggcc atgactcctc aatctttatc
    30181 gtaaaaactg ccacgcccgg cacgggcgcg ccgttccatt tctgccctga ccatttcacc
    30241 gaccagtttc gccagttcgc ggggattctg cgtaacaacg ttatgcagat gaacatgaat
    30301 ttcaccacca aatccggagg caacaggctc ccggttacgg gaagttacag gaactgatgc
    30361 cactggagag cgtatggcct ccgccaccgg gcgggagctg gccgcaacaa cagggaccag
    30421 cgccggaggc agcggagccg ggaccacggg tgtgatatta attgcggggg caggcttact
    30481 gacctgcgca atcttccgct cctgccactc cccacgaaca gcaagtgcgc ggggcaggtt
    30541 cttaaagaca atatcgccgg ggccaatgcg ttttttcgtc tcatcaacca gcttacctgt
    30601 gttatcagca attttgctga gtctgcgtag cgtcccggta ttgctgtctg tgagcggttt
    30661 gttgtctttg ggtttatcac ctccggtgcc attgccattt tccacaggct tcggcggatt
    30721 gattttcgcc aggtccccct gaagcaaggc aaccttgtcc tgaagaatgg ccgcacgctg
    30781 tgcgtcttcg attttcttgc gcgccctttc cgcttcatcc ggaaggacgc caagtttttc
    30841 aagtatccac gccagcgtat ccagtagcat ttttgcaggt gtcagaacaa gttgtaacgc
    30901 accgccaaga acgttaccga atatctcgcc agcactggca catttatcca gcgtttcctt
    30961 gctggactcc atcggtgaca gcagcgattt aaaccagtta aagacctgaa taatcccatc
    31021 gcgcacgaca tcaaaaacag ggccaaaccg ttcaaaggtt tcgcgcaacg gggtcagcct
    31081 ttccataatc ccgctgaaca ccccggcaaa aaatgccctg atgggatccc agtatttcca
    31141 gataagaacg gcagctccgg caagcgcagc cacgataaga ctaaccggac tgaacagcgc
    31201 cccgatagcg ccaccaagta aagaaacgga acccgtcacc attccccata atgcaggcag
    31261 aaccctgaca acattcattg attcggtcag gagagaaaaa ccgagacgca acgtagccag
    31321 cttcccgtga agcaccccaa taaccagaga caacgaacca accgttgctg ttgtcgccag
    31381 cagtgcgcct cccgctatca gtaactggcg cgtcagtgcc gggtgggcct gcgccagcgc
    31441 cgtcaccttt gagacaaccc gcgtgagcca ctgcgtgaca gaacgcagcg gaccgtcaac
    31501 cagatcactg atgcgaatac gcagaccttc ccatgcactg ccgagtgatt tcaggttgcc
    31561 atcaaggtta ttggccataa cctttgccgt gcgttctgcc tcaccgcgcg cgccttcaag
    31621 ttcttttctc agtttggcta agaagccgtc acctgccgca tcaacgaggc ccataaatga
    31681 tgtgaaagcc tcttctccgg caatgtcctt aaagaacgat acccggtcaa cctccccgta
    31741 tttgcgggtg gctttataga gatcggccag cacattctcc atcgggcgca ttttgccttt
    31801 ggcgtcagca actgacaccc ccagctcttt cagtgcttct gccgctgcct ttggcggtga
    31861 tgacagattg gccaggcttt tacgcaacgc tgtacctgca tcactccccc tgatacccat
    31921 cttcgccagc acgcccgcca tcgctgcggc ctgctccagc gatattccca gcttacccgc
    31981 caccggacct gcatatttca tggtttcgcc cagtgcgcga aggtcagtgt tggtacgggt
    32041 aaacgctgcg gtaagtgtgt caccgacccg gtccatctgg tcagcagaga ggccgaactg
    32101 cgtcaggata ttggaaccaa tatccgccgt ctcgccgagg tccataccgc cagccgttgc
    32161 catgctcagc acgccgggaa gcgcagcctg aatggcctgc ggtgtgaaac cagccattgc
    32221 aagaaatgcc tgtccactgg cggcatcgcc tgcggtgaac tgcgtttcag agccaagttt
    32281 taacgcctgc tcacgcagcg ccttaaactg cgggctgttt ttatcgattc gcgtcagtgc
    32341 ctgaacacgg gacatctctt tcccgaaccc gatagcgggc tgcaaaaaac gcccggcagc
    32401 atagccgccc gctgccgccg cacctgttgc cagcgcacca cctgttttca gttttcccgc
    32461 tgtttcctgc gcgcgtgaat accgctcacg cgcctgcgtt acacgcgcaa gcgcccgccg
    32521 ttcgcgttca agctggttgt tgtattgtcc ggtgcgtctg atggcctgct ggatggtgtt
    32581 atcgctgcct gtcagggaaa tgccgtggcg tttcagctct ccgccaagtt cccgcatttt
    32641 ctgaatttcc cgtgtgcgcg attcattcag gcgttcaagc cgggtgctta actgctgcat
    32701 cagcttttgt tgtttttcgc tgagcactgt acccgtgcgt tgtaactgat taagggcgtt
    32761 aagctggcgt cgtgctttca cgatgcccgc atccgcttta ctgacagcgt cacgggcgcg
    32821 ctcaaatgaa cgcgcctgac gctcgagatt tttgatcgcc ccctgcgttc gctggatgga
    32881 gtcaccaaac tgccccatca ggcggcgggc gttttcggca ggccgggtca gcctgtcaac
    32941 ggcgctgaaa gcgacccgga tatcaagagt cttcattgtc tgcattcccg ctgcgaagtg
    33001 ccgcccgctc acgccagcta accacttcgc cgggcgtcat catgaagatt tcggcgggcg
    33061 accagttaaa aatggcggca atatccgcca caaagtcttc tatgtgctca aagcacacaa
    33121 ccgtgatcag gcttccgtcg cctgttcgtt cttctcgcca gagtccgcac cgctcaaaaa
    33181 atttacggca accacacata actgaataaa gtcacgggat gccatttttt tgatcgtcac
    33241 ttcatccagt cgcggtgatg tcacgcgtga cagcagcgta aacatggatt ccgctttcaa
    33301 attcagcaca tcagacagcg acaaatctcg cagagatcca gcctgctcaa tagccccggt
    33361 gatctccaca tacgtgattt tttcgccgcc tcgctcaatt ggttgggtaa gttttacgcc
    33421 acgctcactg gtttctttca cagtgtcagc aacgaccgtg ttttcggtat cgatgttttt
    33481 cgtctctttc atcaggaaac tcctttcagt cagaggcgac gcactgcgcc gcctgcatat
    33541 tacttatcag ccaagcccga gcgcggaacg gatgcgatcg ggcacaatgt ccctgccgtc
    33601 cttccggtaa atgaagttca gcaggtcaat ctcccacaac gggcgatcgt taacactcag
    33661 cttgtagtag gtgtttttaa tggcgtaagt gtgtgatgtg gcttcgccct gtttggcttc
    33721 ccccatatca atttccgtca cacgtccgcg catttcgact tcatacaggt cgctttcagc
    33781 gtcggtgtag tattcacccg caaaacgcag cagcgtaccg tcaatcgtgc cgccatactt
    33841 aaggaacagc tcacgaactg cgccccccat gacaaagctc gcatcaagcg cggagtcatc
    33901 caggccgaga tcaatactta ccgctcccat catgccacca ccccggtagc tgtcggtttt
    33961 gcgcgtcagc ttaggcagag tgacggacgt caccttaccc acttcgtttt caccatccac
    34021 aaacagcgta aaaaagcgaa gatgttttgg tacagccatc aggcacctcc cagcaccgca
    34081 aatgcgggac caaagaattc atcagtaaac gactggtaaa gctccatgtc ttccagcggg
    34141 ggaacaggcg tatatttgta gcgaatacgc acgcgcccct gacgtaaatt cgtggtgccg
    34201 ttatccacca cgtcatacca gcacgacgcc ccaatcagtt tcccggcagt aaccagtgaa
    34261 tccagttttg ccctgatggc accgataaca tccttcacgt tcgcaggcgt cagtggactg
    34321 tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc ggttcgggta
    34381 tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt tgccccagaa gcggaacccg
    34441 ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc actgtcttcg
    34501 gcctgcaacg accagaacac atgcctggac atccccagca catttttaac cggaacgttg
    34561 gacagcgatt tgtgccagcc ctgctcatgg tcaatgtacg ca
//
LOCUS       DAZPJP010000025        34443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPJP010000025 DAZPJP010000000
VERSION     DAZPJP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.025"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..308
                     /locus_tag="ACHU9J_001107"
     CDS             <1..308
                     /locus_tag="ACHU9J_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Shufflon protein C"
                     /protein_id="HFR1904706.1"
                     /translation="PTCQSGTWRKSNSGSTVITGRIANGQQIPLPTGFSASQCSWSVS
                     NAENPQGWKPNYFAGSVATYDANRIVKCGFYDEYNFHKGTFRADLTGKCSYVVACQN"
     gene            881..2035
                     /locus_tag="ACHU9J_001108"
     CDS             881..2035
                     /locus_tag="ACHU9J_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1904707.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2186..3010
                     /gene="traE"
                     /locus_tag="ACHU9J_001109"
     CDS             2186..3010
                     /gene="traE"
                     /locus_tag="ACHU9J_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR1904708.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRRNTTMSLRRVTLRPGLAENLITGQILPVSLFNTSLLPW"
     gene            3096..4298
                     /locus_tag="ACHU9J_001110"
     CDS             3096..4298
                     /locus_tag="ACHU9J_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR1904709.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4358..4942
                     /locus_tag="ACHU9J_001111"
     CDS             4358..4942
                     /locus_tag="ACHU9J_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFR1904710.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEMN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPAYLDALIMHYDGTRLILDGKYNKEA"
     gene            5337..5795
                     /gene="traH"
                     /locus_tag="ACHU9J_001112"
     CDS             5337..5795
                     /gene="traH"
                     /locus_tag="ACHU9J_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFR1904711.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            5792..6610
                     /gene="traI"
                     /locus_tag="ACHU9J_001113"
     CDS             5792..6610
                     /gene="traI"
                     /locus_tag="ACHU9J_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFR1904712.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLISTEGGSR"
     gene            6607..7755
                     /gene="traJ"
                     /locus_tag="ACHU9J_001114"
     CDS             6607..7755
                     /gene="traJ"
                     /locus_tag="ACHU9J_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR1904713.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            7752..8042
                     /locus_tag="ACHU9J_001115"
     CDS             7752..8042
                     /locus_tag="ACHU9J_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR1904714.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            8057..8608
                     /locus_tag="ACHU9J_001116"
     CDS             8057..8608
                     /locus_tag="ACHU9J_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFR1904715.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQYLEHWQDRWNKGTDWRP"
     gene            8698..12465
                     /locus_tag="ACHU9J_001117"
     CDS             8698..12465
                     /locus_tag="ACHU9J_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR1904716.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINTQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNIQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYENYSAYQELMNNENPEHLQENSDMPSQPDTDHVQADESELQATTDTAE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHKPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTV
                     STVPASSPAEPGKTADAAQGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEMKTLRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            12483..12830
                     /locus_tag="ACHU9J_001118"
     CDS             12483..12830
                     /locus_tag="ACHU9J_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR1904717.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPRKDYNL"
     gene            12827..13519
                     /locus_tag="ACHU9J_001119"
     CDS             12827..13519
                     /locus_tag="ACHU9J_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR1904718.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTNQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            13530..14513
                     /gene="traN"
                     /locus_tag="ACHU9J_001120"
     CDS             13530..14513
                     /gene="traN"
                     /locus_tag="ACHU9J_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFR1904719.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            14516..15805
                     /gene="traO"
                     /locus_tag="ACHU9J_001121"
     CDS             14516..15805
                     /gene="traO"
                     /locus_tag="ACHU9J_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR1904720.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGVGYIAFPKISGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            15805..16509
                     /gene="traP"
                     /locus_tag="ACHU9J_001122"
     CDS             15805..16509
                     /gene="traP"
                     /locus_tag="ACHU9J_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFR1904721.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            16509..17036
                     /gene="traQ"
                     /locus_tag="ACHU9J_001123"
     CDS             16509..17036
                     /gene="traQ"
                     /locus_tag="ACHU9J_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR1904722.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            17087..17491
                     /gene="traR"
                     /locus_tag="ACHU9J_001124"
     CDS             17087..17491
                     /gene="traR"
                     /locus_tag="ACHU9J_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFR1904723.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            17555..17743
                     /gene="traS"
                     /locus_tag="ACHU9J_001125"
     CDS             17555..17743
                     /gene="traS"
                     /locus_tag="ACHU9J_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFR1904724.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            17727..18527
                     /gene="traT"
                     /locus_tag="ACHU9J_001126"
     CDS             17727..18527
                     /gene="traT"
                     /locus_tag="ACHU9J_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFR1904725.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            18617..21661
                     /gene="traU"
                     /locus_tag="ACHU9J_001127"
     CDS             18617..21661
                     /gene="traU"
                     /locus_tag="ACHU9J_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFR1904726.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPQERKDEAVGIILSND
                     PDYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            21661..22275
                     /gene="traV"
                     /locus_tag="ACHU9J_001128"
     CDS             21661..22275
                     /gene="traV"
                     /locus_tag="ACHU9J_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFR1904727.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            22242..23444
                     /gene="traW"
                     /locus_tag="ACHU9J_001129"
     CDS             22242..23444
                     /gene="traW"
                     /locus_tag="ACHU9J_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFR1904728.1"
                     /translation="MQRKTLLAALIAILNGTAYQAHAYSVTVVASRPVEQQVVPRMEA
                     IKGVLSDILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            23473..24057
                     /gene="traX"
                     /locus_tag="ACHU9J_001130"
     CDS             23473..24057
                     /gene="traX"
                     /locus_tag="ACHU9J_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFR1904729.1"
                     /translation="MNKLPENSDQTKNVKKSLSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            24154..26322
                     /locus_tag="ACHU9J_001131"
     CDS             24154..26322
                     /locus_tag="ACHU9J_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR1904730.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
     gene            26396..27046
                     /gene="excA"
                     /locus_tag="ACHU9J_001132"
     CDS             26396..27046
                     /gene="excA"
                     /locus_tag="ACHU9J_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR1904731.1"
                     /translation="MQMVKTINRYPAWWTLYYALRFIYFISIPVLSIFILFGVLSITS
                     SRYVTQEDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEG
                     LSFTQGVYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINI
                     PMVSIPSGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(27073..27327)
                     /locus_tag="ACHU9J_001133"
     CDS             complement(27073..27327)
                     /locus_tag="ACHU9J_001133"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFR1904732.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYRQRHKLSVVMNKSRLI"
     gene            27719..27895
                     /locus_tag="ACHU9J_001134"
     CDS             27719..27895
                     /locus_tag="ACHU9J_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904733.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(27960..28055)
                     /gene="dqlB"
                     /locus_tag="ACHU9J_001135"
     CDS             complement(27960..28055)
                     /gene="dqlB"
                     /locus_tag="ACHU9J_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR1904734.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELVD"
     gene            28554..28805
                     /locus_tag="ACHU9J_001136"
     CDS             28554..28805
                     /locus_tag="ACHU9J_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1904735.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(28877..29029)
                     /locus_tag="ACHU9J_001137"
     CDS             complement(28877..29029)
                     /locus_tag="ACHU9J_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1904736.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            29321..30529
                     /gene="trbA"
                     /locus_tag="ACHU9J_001138"
     CDS             29321..30529
                     /gene="trbA"
                     /locus_tag="ACHU9J_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR1904737.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            30548..31618
                     /gene="trbB"
                     /locus_tag="ACHU9J_001139"
     CDS             30548..31618
                     /gene="trbB"
                     /locus_tag="ACHU9J_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFR1904738.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGKTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYGDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVAEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            31611..33902
                     /gene="trbC"
                     /locus_tag="ACHU9J_001140"
     CDS             31611..33902
                     /gene="trbC"
                     /locus_tag="ACHU9J_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR1904739.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETNEENQISVRAERASINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(33975..>34443)
                     /locus_tag="ACHU9J_001141"
     CDS             complement(33975..>34443)
                     /locus_tag="ACHU9J_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFR1904740.1"
                     /translation="EERFQPISGRVVFRPVIYSEELFREYVREALHDYINRSSLNIKC
                     ARGILHASLIRLCSLYKRESFECENEVRGLIEVDEHTSPPYKLEKRSTDYGIAYYTKV
                     KFTCNNITAIREIVLGPLFEQSDEQLRLKLDDLGLKNIKIRRSICTYNDAVNL"
BASE COUNT         9413 a         8712 c         8239 g         8079 t
ORIGIN      
        1 ggcctacgtg ccaatccggt acttggcgta aaagcaattc tggtagtacg gtgataactg
       61 gtcgtatagc aaatggtcag caaataccgc tgccaacagg attttctgcc agtcagtgct
      121 catggagtgt gagcaatgca gaaaaccctc aaggctggaa gccgaattac tttgcgggat
      181 cagttgcaac ttatgatgca aatcgaattg ttaaatgtgg tttttatgat gaatacaatt
      241 tccataaagg gacatttaga gctgatttaa ccggaaaatg tagttatgtc gtcgcgtgcc
      301 agaactgaga ttaaattgct ggcgctgctg gcctacgtgc caatccggta cctggggcgc
      361 tcctaaaatt caattcacaa cgcagaccta caacatagct aaaaacactc gtaatttaag
      421 gctcggggtc catgcatatt gctcatggac ttacctgaat ggctcaccct ttggtggttt
      481 tcaacaggta tattccgacc aaaacaacgt ttggtatgtg agtaattatg cttggggaaa
      541 ttatgagtct ggtgggacca tatcagtcac atgcctcaat cttcctggtg ctggagctta
      601 attaaagggg caacagtagg catcgacatg ttcctcatca acaccatcac caataccgta
      661 catgcgtgtt ccagtcataa tgtatccagt cggacattga atcggcttgt aatagatcga
      721 accaccactc ttaccgccga taaagtgatt cattgctacg gatgatttgt gccaattaca
      781 tgctgagtaa ttaattttat taccccctga ccaccgaccg gattggcacg cttgcagaga
      841 atgattgttg gcattatttt ttgcctcttt tgaggttagc atgccgtctc cacgcatccg
      901 taaaatgtcc ctgtcacgcg cactggataa gtacctgaaa acagtttctg ttcacaagaa
      961 agggcatcaa caggagtttt accggagcaa tgttatcaag cgatatccca ttgcccttcg
     1021 gaatatggac gaaataacaa ccgttgatat tgctacatac agagacgttc gtttagcaga
     1081 aatcaacccc cgaacgggta aagctattac aggtaatact gtacgtcttg aactcgccct
     1141 tctgtcatct ctgttcaata ttgctcgtgt tgaatgggga acctgtcgta ctaacccggt
     1201 tgaactggtt cgcaagccga aagtatcctc cggacgagat cgccggctaa cgtcttcaga
     1261 agaacgtcgc ctttctcgct atttccgcga aaaaaatctg atgttgtatg tcattttcca
     1321 tcttgccctt gaaacagcca tgcggcaggg cgaaatactg gccttacgtt gggagcacat
     1381 tgatttgcgc catggtgtgg ctcatttacc tgaaaccaaa aacggtcact cacgggatgt
     1441 tcctctgtcc agacgtgccc gtaactttct tcagatgatg cccgttaatc tccacggcaa
     1501 tgtttttgat tacaccgcat ccggctttaa aaatgcctgg agaatagcca cacaacgact
     1561 tcgcatcgag gacctgcatt ttcacgatct acggcatgaa gcaataagcc gcttcttcga
     1621 actgggtagc ctgaatgtaa tggagattgc tgcaatatca ggacatcgtt ccatgaatat
     1681 gctgaaacgg tatactcatc ttcgtgcatg gcaactggtc agtaagcttg atgcccgccg
     1741 gcggcagaca caaaaagtgg cagcatggtt tgtgccgtat cctgcccata tcacgaccat
     1801 cgatgaagaa aatgggcaga aagcgcatcg tattgagatc ggtgattttg ataaccttca
     1861 cgtcactgcc acaacgaaag aggaagcagt tcaccgcgcc agtgaggttt tgttgcgtac
     1921 actggccatt gcagcacaga aaggcgaacg tgtcccatct cccggagcgt tacctgttaa
     1981 cgaccctgac tacattatga tttgccctct gaacccgggc agcaccccgc tgtaaacatc
     2041 attcaaaaca catacagaaa ggcgagttgc aacaactcgc caaacacatt cgatctgcat
     2101 gatcgaaatt attcgtttca ccagaaaaaa caccgaagat acaataacca catgagctga
     2161 aaagactttt tcagtcggag gaacaatgag actgaatacg accggtattg cggcccgaat
     2221 gatgctctcg ctggacaaaa aagcaatcgg cgaaaaactc atcaacggtc gtcagataaa
     2281 cccgacacct ctgcaggtcc gttacccaca aggggtaaga gaagttctgg ggataatgag
     2341 cgaacaatta gccatatcca ccgctgattt aacccgaatc ctgcttgagg atgctcttca
     2401 taatatgttt atgcctgcgg ataacaccac aggaaacata ataagcagaa ttgaatacat
     2461 catgctgtcg catgatataa atgcgccatt actggcaaca ttgctgtcgc catggaatat
     2521 ccgctccacg gttatacagg acccggccag acttgcagac tatctttcag ctgacgctct
     2581 cgaacatctt gctgaatgtt ttaatctcaa tccggactgg ctgaatggcc atgaaaacta
     2641 tccgatagcg ttgtccggtg aatggccaga tactgctgat aatttcagaa tgcttattaa
     2701 tgatagcagc aacactgaag ttattttctg gcatagtttc cccttcgcag gaaacacgaa
     2761 aagagaatat tgcggggtaa tattgagaca ggaaaaagaa atcaatggct ctgtaatata
     2821 tccggcccta tcattatcac caacacttct gaacgatgaa aaaagaaaat ggttaactga
     2881 atatacaacc aggcggaata caacaatgtc tttgcgacgc gtcacgttga gacctggcct
     2941 tgccgagaac cttattacag ggcaaatact acctgttagc ttatttaata cgtctttatt
     3001 accctggtaa ataccagata gtaccaaagt aaaaaattac ggcacatccg tgtcatagcg
     3061 ttgttttgtc taaattctga taaacaggaa attaaatgat gaagaaaaat catattaccc
     3121 gcaccatcat tgcatctgct gtattatttt cctttaacgc tgcagcagcg acgtcatatt
     3181 ttgaagcgcg taacgatgca atgggtggca ccggggtagc atcttcccat tatggtgtgg
     3241 cacctctggc aaacccggct ttactgacaa agcacaacag caatgatgat ttcagccttc
     3301 ttctgccgtc tgttggggca caagttgctg atcctgatga tgtatcaaat aaagctgatg
     3361 atgttaaaga tgactgggat ttgtttgaca gtgctgtaga taaccagcat ggcgtacaac
     3421 aggcagcggc aaatcttaaa catcgtctgc aggaattccg caatatcaat gcagatgctc
     3481 aggtaggtgt atcagctgta gcagccatgg caaatgacac tctgccgttt gcccttatgg
     3541 tcaagtctta cggaactgtc agcgtaaacg gaaaagtaaa cgatgcagac ctggattatc
     3601 tggacaaagt tgccaatgga acaattaccg acgtcgataa gaatgccctg acctcacgcg
     3661 cattcgggcg tgcagctgta attaccgacg tcggaatttc atttgccaaa gaactggaaa
     3721 cggccggaca aaaatggtct cttggcgtca caccaaaata tcagcgtgtg gacctgttta
     3781 actacaacgt aacggttcgt gattacgaca aagacgattt tgacggtgac aagtatcaca
     3841 acacgaaaaa cggattcaac gccgatattg gtgcttatac agacctgaat gacaactgga
     3901 ctgtcggtct tgttgcccag aacatcattc cacgcagtat tgatactaaa gtcgtcaatg
     3961 gctttaaaga aaccttcaaa gttcgtccac aggcaaccgc cggtgtatcc tggcataatg
     4021 acctgtttac cactgctctt gacgttgatc tgactcctgc cagtggcttc acttctgaca
     4081 gcaaacgtca gtttgcaagt gttggtgctg aatttaacgc atggaaatgg gcgcaattgc
     4141 gtgccggtta tcgtcagaac atggcatcaa actccggaag cgccttcacc gctggcgttg
     4201 gtatctctcc atttgacgtc gtacatatcg atgtttccgg cctggtagga actgaccacg
     4261 attacggtgc aatggcacaa cttcagttca ctttctgatt actgtccccg cccccggcac
     4321 taaacccggg ggtattttta tattcatttt aaataacatg atgaaaccac gttcttctta
     4381 ttcaaagaca gcattcattc ttttattttc tgttttcctg gtagctgcag tgacaaaagc
     4441 aaaaagctcg cttcccgata tcaccctgga acaggcaaaa gaaatgaatg ctgataacac
     4501 cgttattttc cttttccgcc atggagaacg ttgcgaccgt tcagatatgc cctgttattc
     4561 ggataagagc ggaatcacta ttaccggtac agagaaagca cagcaggaag gtattaagtt
     4621 cgcaacaatc ttcagtgaat atgatattta ctccagcaat gcggtaagaa ccatacaaac
     4681 tgcaaaattc ttctccggta aagaacccgt cgttatggac agtttatcag actgcaataa
     4741 tgatctttat aagacgcttg aatcaatcgc aagagaatct cataaaagga acatagtaat
     4801 aatgacacat aatcattgtt tatcatttct ggccagagat aggctcggca agaaattcaa
     4861 accggcgtac cttgatgccc tgatcatgca ttacgatggt acccggctca tacttgacgg
     4921 aaaatacaac aaagaggcat agaggatatt tgaatttaca taaattctga tatactcagt
     4981 aagatacaag agtgtacggg cacatgtgcc tgtctgaatt gcaggttatc cggacgaggg
     5041 ccgggaactg tttcagggac aagcgccggt acgttcgatt cataaatcgt cgagtcatcc
     5101 atcatggatc gattcggcgc ggagcgttaa agattacgct catattcgtt ttatgtctga
     5161 cgacacatca accagccagt atcaggaata atcaccttca gtaaactatg aggctattta
     5221 cctggccaca ctggcattta tttccggtaa cgcaagtata gccggaaaca aacatcatcc
     5281 tgtctgtacc ggagcaccgg tatatcccat tctgatttaa ctgctcagga attattatga
     5341 accgtattat acctgcgggg tgtcttgccg ccctttttct ggctggctgc aactcccctt
     5401 ctcagcatac tcctgttccg cgtaactacg gtgctgcctc tgatgtggcg gttacccgac
     5461 acactcagta cacattatgg gacaaaggtg tgttttccgg ccaacttccg gtttcaacgg
     5521 ggtctcatct tctgacagct aacagcgatc gcctttcctt tgactgggaa ggtgatgcga
     5581 ttgaattact gaacgaactg gccagagtca gggggatgca gtttaactac aatggtgtcc
     5641 gcctgccgtt accagtcaac cttcacgtcc gggatatgac gttttccaat acgttgagac
     5701 ttattgaagc ccagacagcc tggagagcaa caatccatca atatccgggc ttgttacagg
     5761 tcagcttcat gcagccggag aaccggaaaa aatgaaagca ctcacctctc tcattttgcc
     5821 agtaatactg cttactggtt gttcttcttc ccccaccatc tcgccaggtg gtgtcaatac
     5881 gccaccacct gacattaatg catggcttaa accacgaagt gaaaagccgg atagtctgtc
     5941 agaaacacgc tggaaaatgc tgaccgacgc aggtaaaacg cttggatttc gtggtggcaa
     6001 ggctcagcgt tcatgggagc tgattcaggc actcaacgcc cgggaaagca cacttaatgc
     6061 cttatacgat ttccggccac tgattagccc ggaaggctgg ctaccaccgg ttattgatga
     6121 agcacaggac gtagctcata tcacgccaga tcaaatccgg acctcttccc gggtctggac
     6181 catcattcgc cctgaacgct ttgtcagcaa tccaccgggc tggcgtgact ggcttctccg
     6241 ggggctttca acaacagcca ctccgggtac agaaggcagc gtcgttccag aagacagcgt
     6301 gcaacgaaaa gtgtgggaaa ccgcattacg gcagggatgg caggaaggaa gacaaaacgc
     6361 cgacctgacg cttgaagcca accagaaaac cctcacccgt gattaccggg gcatgatgct
     6421 gtactcactt ctctggcgtc agggcatgat cacccgtccg gatgtttctg atcaaatgca
     6481 aaccgttaca ggtgacggga aaaaactggt cactggagat cgtgttcgtc gccttaaaaa
     6541 tcatgcggaa ttcaatcttc agaaatctca ctggcgtccg cttataagca ctgaaggagg
     6601 ctcccgatga atattcctga tgagtttggt ctttttccgt ttcagcgttt cacggcagat
     6661 gaattacggc atttctttgt ctggtgtgcc gcacataaag tcagtgatgt ggatctgacc
     6721 ggtgggtcac ctgtatctgt cagtcgcttt ggccgacggg tgcgttgttc cagtgcaacg
     6781 ttaccaacca cacttatgtc ctccctgatt gatgaactgt tcggtcggga agtcattccc
     6841 cgcgttctgg caggcaatcc ggttgacagg acgatacaga ttaacgggga tgcatccggt
     6901 cgctatggtc ttaaacgcgg agaacgcatc cggttacgct ctcacctcat tcagggcaca
     6961 tctggtgcgg aggagaaagc aatatcaata accatgcgtg tcatcccgac agaaataccg
     7021 gacatcctct ccatgaacat tgaaccagac ctgcttgaag caatggtctg caaaagcggt
     7081 ctgggatttg tatgtggtga aaccgggtca ggaaaatcaa cgctgtgttc agcactgtac
     7141 cgttacatta tggacaattt ccccgacgcc aaaatcgtga catatgaaga cccggtggaa
     7201 tatatcctgg gaaatgaaaa cgatctgctc ccaccgcatc aggcagaaat cgggcgtgat
     7261 gtggtcagtt ttgctgccgg actgcgttct gctgtacgac gtaatcccga aattatcggt
     7321 gttggtgaga tcagggataa cgaaaccgcc gacgcagcgg tacaggcagg caacaccggg
     7381 cattactgcc tgagcaccat gcatacgaaa tctcctggtg aaacgctggc acgtcttctt
     7441 gggctgtttc cccctgtcat cagggattcg atggcctggg ctgtcctgtc actgttgcag
     7501 ttcattctgg ttcaggttct ggtcagaaca aatgacggtg gtcgtaaagc cgtccgggaa
     7561 tatatcgtca ttaacgatga gcttcgcgac aatctttccg gaatgccgca tgcagagtgg
     7621 gggcatcaca ttgatgccat aatccggcag gagaagcgcc gtatccggga ccagattctg
     7681 gaaatgtata tccggaatga agttgaccga agagaagcga ttctttttat cccgccaggg
     7741 gagttacggt catgaaaaga gaaggtttct ggcgatatgc aacaccgcca gtcaacatcc
     7801 agggcgtacc tctgccgttc accatgattt atctgtttat atgccccttt ccctccaaaa
     7861 caaccttctg gatttgcacc ggcatcattt tatttttcgt tattctcgac cgttcaggct
     7921 ggactgtcag gacgatctgt actcgcattt tcagcattct gagaggggcc atcgcctccg
     7981 gtcgtccatg gtggtatcgc caccatacgg aatcaccacg agactggact ggcctcgatt
     8041 aaattctgga gtctttatgt caggtaaaaa aactatcgtt actctgctcc gggtatcgct
     8101 actggcatgc ccgttactgt tcacgacacc atcgtttgca atgatcgaca cgccatccgt
     8161 gaaggtcggc ttttctcctg aaggaagcgc atccgctctg gtgcttgaca caatcaacag
     8221 tgcggaaagc tccatcagga tgatggcata ttccttcacc gacccggatg taatgcatgc
     8281 gctggcaaaa gcgaaaaaac gcggagtgga cgtccgtatt gttgttgatg acaaggggaa
     8341 tacgaaccgg gcaagtcagg aagcgatgaa atatatcaac ctgctggaca ttcctctccg
     8401 gactgtagat gccttcccca tccatcacga caaagtcatc attgttgacg gaaacacggt
     8461 tgaaactggc tcctataact tctctcgtgc tgctgcacgc aagaattcag agaatgtcgt
     8521 tgtgctcaag aatatgccgg atgtagccgc acaatatctt gaacactggc aggatcgctg
     8581 gaataagggt acagactgga gaccctgaca aacatttctc ccccgatttt ctatcccccc
     8641 agccaccccc ggcttccggc ggtggttttt ttccgtcttt tttcaggaga agcactcatg
     8701 ccgtctgtat cccgctaccg cacctggctt gccgtgcctg cagatgaaat agaagatctc
     8761 aaaaaagcac atcccccgat gaacgggcac acgcccgtca tctgggacaa ggagcacaaa
     8821 ctctggttcg cccgcccggg ggcggacctg tccaggctgg accgctggct gccccggcca
     8881 caggacgtct ccatgaacgg cagtgatccg gtcaccgagt ttgcccaggt tctggagaat
     8941 gccggactgg ttctgaaaga actgccggtc atggacggga aaatccaccg tgtcccgacc
     9001 gccgatgaca aaaaaggaca aaaaagtggc gcatatcgcg gattcctgga cggcagacct
     9061 gccggctggt acagggatta tcgcagcgcg gataattcgc cgatcacctg gaccttctcc
     9121 ggcggagaac agacagatcc ccgcgcaaga ctccacctga aagcgcattc aatgcaacgc
     9181 agggaggacg cggagcggga actgaaagca cagtacaacc gacaggccgc gtatgcccgc
     9241 cgctatatca acaaatggcc gcaggccaca gcacatgaat acctgacccg gaaaggcatt
     9301 caggctgcac cgggggtgcg ggtaaacaac aaaaatgaac tggttattcc cttcagtaac
     9361 cgtaacggcg cgatacgctc ttaccagcgt attccggtca ccggggggaa agatgcccgc
     9421 atcctgatag attcggagaa aaccggtaac tggtttgcgc tgggcacacc gcgcaacggt
     9481 cagccggtac tgtttgccga gggctatgcc actgcggcct ccctgcacga agccacgggc
     9541 cttccggtac tgatgacggt ggatggaggc aacatgattg ccgttgccga aaatgcccgg
     9601 caaaagtgga cgcaaagccc ctttatcttc tgtgccgaca acgatcatgc aatccgggtt
     9661 aacaaaggta tcgtcagtgc aacaaaagcc gcagaactga caggaggatc ggtcatcttt
     9721 cctgcgttca ccgatgccga aaaagcgcag gggctgaccg atttcaatga cctggacgca
     9781 agccggggac gggccgcttt tcagcatgtc atcaacaccc agttagaaca catcggggtt
     9841 tccaccccca acagtaacac cccggaaatg cgcgaggcac tggtgatagg caacctcgtt
     9901 tttacaccgg tccacactga ggaaaaaacc atgactccga cagaatatcc tgaaacatca
     9961 cccgatacag ggcacagcca tgaccaggga ccttctctgc cgtccgccac gcagcaggaa
    10021 caacctgctg cttccagcag taacattgct gatgaaacac agtcatttac cagtcacgcc
    10081 actgaaaata acggcaaaga tgagcggcac gctgataaca ttcaggcgca aacggaaagc
    10141 tctgtagttc aggactcccc tgcagaatca ccggcgtccc cggaaagtac aacggcgtct
    10201 gcccctgatg aacccgctca tccggcggaa cctccggaaa aagttgtctc ggtggccaca
    10261 gataaagact ggcgtgaatt tgaagccgaa ctgtcacaac cggaaaaaaa cgagtcacaa
    10321 caggaatccg gcacttcacc tgaaataccg gcccctgctg ccgcccccgc atcccctgaa
    10381 gacagtccct catctgaaac catgaatgaa tcagccgagc catcaccttc ccctgatgca
    10441 gtcaaagagg aaatgaccgt gatgacggag cctgatgaca aacaaagcac cggaatatcc
    10501 ccggatactc cccgggaaga ggcggtcaac catgtaactg aagacattcc ccttcagcca
    10561 gagccgttcc ttcctgagtc agatggcccc gaagaatacg aaaactacag cgcatatcag
    10621 gagctgatga acaatgaaaa tcctgagcat ctgcaggaaa acagtgatat gccgtcgcaa
    10681 ccggacacag accacgttca ggcagatgag tcagaacttc aggccacaac agatactgca
    10741 gaaccggctg tccgggatga acccactcca cagcaacctg ttcagagcac atcaccttct
    10801 gataatactt catcgtttct cgacaaagcg cgtggctttt ttacgcgtaa aaaaacggat
    10861 tcacaggcac atgagaacag tgatcccacg ccctcagcgg aaaccacgac gactccaccg
    10921 atacctgaca gtattgtcta cgccccggaa cgttctgatg caccgatatc gctcaacatg
    10981 gacgaaattc ttaaagagct cgactgggaa aaacgagcag acagaaccgt cctgtacaaa
    11041 ctggatggca aacctgcctt cattgaccgg gtaaaccggc tggaaatggt caacggtgcc
    11101 agcaatgacg atcgcagcgt tctggccgca cttgctgtgg ccaccaaatt ttatgggggc
    11161 gtcatagagc tgacgggcag tgatgccttt aagcaaaaag caatgcagtt gattatcgag
    11221 cacaacatcg atgtccggat gaaattgcca gatcaacggg ctgaactaga aaaattacgc
    11281 aaggaaatgg ctgtcagtaa ggatgctgtt gtcacccata agccaacacc ggaacttaac
    11341 cggaataccc ctgaacaacc agcagtccca gaccctgttc aggaaaaaga agccacacaa
    11401 tccccggcgt ccccagtagc ccctgaagca tcaaccgtca gcaccgtccc tgcatcatca
    11461 ccggctgaac ctggcaaaac ggcagatgcg gcacaaggtg aagaaccacc aggaaaactt
    11521 cgcccgggag agagcgtgac tgccgtactg cacaattttg gtcgtgctga atatgcaccc
    11581 ggcaaaggag agagtttctt tgttgagctt aaaaaccgca gtggcagcaa actctactgg
    11641 ggggagcaac tggaaagtct ggtcaaaaac catcagaaag gtgacgtggt gacactgaca
    11701 cttcagaacc gcgaacaatt catcctcccg ggagagcaaa aagcacgttt ccggaataag
    11761 tggtccatgg agtcagttac caacgggatc tcggtatcac atgataatcc ggataaaggt
    11821 cagcgtattc aggcgattcc ggtcgaaacc tttatgaaag tggctgcgca aatcagccag
    11881 gggtggcccg aagagatgaa aacgttacgt atgccagaaa acgtcggctc acatctgttt
    11941 atcggtgaag accgacatcc ggtctccgcg ccacagaacg caaatcaggt aacagaaata
    12001 accagcgcag cccctgacaa gctgacacct gttctgggat cagtggataa ggatacgcgt
    12061 gaactcaacc tgctacttgt ccagagtgcc gatgaacatc tgcagggtgt ggtccggctg
    12121 aacggcacat tgtatccggc actggccacc ccatcagccg acaacagtca gctggttatc
    12181 aatgcgctga ccgacaaagg tttacgcttt gcaggttatg gtgaagcggt aaaccacgat
    12241 gctgacagca cgaatcgccc cgcaccggag cttatgcagt ttcatctgaa aacccgggaa
    12301 gaaccgcttt ttgctgctgt ctacacgccg gaaaaacaac ctgatgcgtt gtaccggaac
    12361 ctgggcttcg agcagtcatg gcagcagtgg tccaacagcc aaaagccgga ggacaggcag
    12421 gaaaaaactc tgcatcagga tctctcccac agccccggcc gatgagccgg taaggaagcc
    12481 atatgaacat gcataagtct ctggttgccg tgctcggcct tatcctgatt tcatccacgg
    12541 caagtgctgc aacctgtcgt gcgggtagcg cggcacaggc cggtagtaat acaggatatg
    12601 agcgagcacg tgcagctgcg gatgcatggt cacaacgtga aaacgatgtt tcctcatccc
    12661 tgcagtcctg tctgtcacga atcaaaaaaa tcagcataaa cctcccccag ttccccagcc
    12721 ttgacgacat tctgtcgcag ctggaaaacc aggtctgcga cgcggtcgtg gacaaggtga
    12781 atgaaaagct gccggggaat attgatccca ggaaggacta caacctatga ccgaaaatac
    12841 cgagccatcc tcgccatcac atccggcacc tgacccggaa atactgaaga gcacaatcag
    12901 ggctatgaag cagtcagaac agcgggcaaa taatgtccct gcgctgataa aagcgctttt
    12961 atgtacgggc acatgtcttc ttatcagtat tactggtaac gccattcagt actggcactc
    13021 aacgaatgtg gagcgcgaat atttcgctac agacaatggt cgtctggtca ggctggcacc
    13081 aacaaatcag ccagcatgga gtcagaatga cgcaatggcc tttggcagcc aggcactggc
    13141 gaccgcattc aacctggatt ttgtgcacta tcgttcacag atttcctctc tgtctccgcg
    13201 tttttctgac gaaggttttg ccggatatgt gaatgcatta caggcatcta acattctgga
    13261 gacgatcaag aaagagaaga tgaatctgac cgccacgaca ggcgctggcg ttctggtacg
    13321 acagggacaa atgtccgacg gcgtatggtt ctggacattt cagtatcctg tccgtatgcg
    13381 tctggtcggc cagacaacca gcaagccaga acagtctttt gttttcgaaa tcaccatcca
    13441 gcgtgttgac cccagactga aaccatccgg tatggaaatc cggcagatga tctcccgtaa
    13501 cgccggaccg aactcctgag ataagtaata tgcaatcacg ttatttactg agcacactgc
    13561 tcctggtgtg cagcgccgcc acgtctgcag ataacgcagg atggcagaac gcacgcacac
    13621 cacaaacgac aaattctgcc agcgatcacg ttcagaacgc ctcccaaaac accggaggtg
    13681 ttccggcaac actggtaaaa ggtgagctgc ctgcccccgg gcaggccagc ccgctggtac
    13741 aggatgcggc aagacttgat tctgagctct ccgccgatga gattcgctca ctacgcagtc
    13801 tcatggcgga taacgagcgg gccatcaatg cgcccatcac cagtgttgta ccccgtatca
    13861 gctctctgac agttaatctg tcccccggtg ccagccttcc tcttgttcgc acagccatga
    13921 acaacctgag cgtagtgaca tttaccgata tcaacggttc accatggcca caatccgatc
    13981 ctccttataa cgcggcacca aaactctttg atgtgcagta caacgagaac atggtgacaa
    14041 tcacaccatt gcggccatgg gcatccggaa acatttctgt ctacctgaaa ggtctgtcgg
    14101 taccggtcat cctgaacgta accagtggcg aaacagatac gccgtcatcc agtcaggaaa
    14161 tggacagtcg tctggatctg cgaataccac ggcagggacc aaccagcccg gttgtctcca
    14221 tcccgacgga taaaatcgcc ctgcatgatg caacattaca agcatttctg gatggtattc
    14281 ctccccggga cccttcagtt aagaggctga aattcacagg taatgtcccg gatacaacga
    14341 tctggcaaca cggcgatgac ctactggttc gttcccgggc gatgttgcgc gatgaatttg
    14401 aacagacctt gtcctctgcc gacggaactc atctctggaa acttcccgtc accccactac
    14461 tgactttctc tgtgaatggc cagtccgttc acgtgacacc agaactggag taatcatgag
    14521 tgctgaacag gatgccggta aatccggaaa gaaactggct gccctacttg gactgggcag
    14581 catcatctta tttggcgtcg gctatatcgc gtttcccaaa atatcaggga gtaacagcga
    14641 tatgcagtca gccgtgaaca ttaacagtgc agcaagcggc ggaacgcgca gcgttaccga
    14701 aacgcctcac tatcgtgaac tgttaagagc tgataatgaa cgaggggccg cagctgcagc
    14761 ccgcaataat cagacgttca ttgccagtct tccacagggg cttgatattc cggacactcc
    14821 ggaaaagcaa caacagcctg cagcaaaacc ggaaaactat gctcatcgcc aggcttccgg
    14881 cacaccacag gaggatcgag cagccagtga aaaacgtatg gagcggctgc aaaagctgat
    14941 tgtaagaata aaagatcagc atcctgccgg tagtacacca accatagcaa cggctatgtg
    15001 gaataaatct ccggcagaaa caacagggca gaatggtacg cagcaattcg cattacagaa
    15061 tgccagcctg tccacgccgg tagcagaaaa agggatacaa ctgatacctg ctctgacccg
    15121 tataccagca tatattgata cggcagtgga ttctgataac ccgtcatcca aggttatcgc
    15181 aaccattccc gctggcccct gggcaggggc aacgctgttc agtccgggag tcaaactggt
    15241 gggtaacggc gtggagatcc atttcgaccg gatgagctgg aacggcatgg accttaaggt
    15301 caatgcatat gctcaacggg aagacaacct gatgtcatcc gtggccagca atgtgaatac
    15361 acgctggttt aaacatatca ttctgccgtc agtgttagga ggtgtgggca gtattggcac
    15421 actttataaa gatgccaaca cgcaggttat tcagggtaac tatggcacgg ttaccggacg
    15481 cgtgggaatg cccagtggtg aagctgttgc gggcgttatt gccggtggta tggcggaaag
    15541 aggaagtcag atattaaccc gccaggcaga aagtgagccc tataaacagg tagaagtgta
    15601 tcagcatgaa gtcgtatcaa ttctgtttgt tgacccggta atgacaaacg acgccaggtc
    15661 atcttccctt tccagtggca tttccccttc agttaatcgt acatcacaga cggaacaacg
    15721 ctcgcaggca cgaatgcaga cagccatgga gcaaagaaaa gctgtgatgc aacgtcggta
    15781 tgatgaacaa ccggagacac cataatgaaa cctgaaacag aaatagatga aaccggcagc
    15841 ttcggtgaac ccgaagagaa acccgcccct ttctggaaac gttccgtctg ggggatatct
    15901 gtagcaacat gggggctatg tgcagtcgta ttgctcgcag ccatatggta tctgttcctg
    15961 agagcaccat cggaaacagg catgcctccg tttaatgatg cggatgctgg cgtacagacc
    16021 tggcaaacca cacaagaatc atcaccttcc gttcagtcca ctgagaccat gactgtccgg
    16081 acaggagaag atatgagcca gcttgcccgg gatgtaaaaa cagagctgga taaccgcgat
    16141 gaaaaaattc aggcaacact gaatatgctt cacgacagca ttaacaagct gggcgaagct
    16201 ataaaaaagg atgaagaata cgcacaggaa acgcgccgtc agctggatga tatccggagc
    16261 cacctgaacg gaatgatgac acagaaaagc gtcaccgaat cctcttccac accgcatcca
    16321 gcaaagaaaa aaacgtcgtc tgtacttaac ggaatgaaaa tcatgtcgat ggaaactggc
    16381 atggcctgga ttcgctggca gggtagtacc tgggctgtca gagaaggtca gactctgggc
    16441 aatgtggtga tccagcggat tgatccgacg acccggacca tcatcaccag tgcagggacg
    16501 ttacgctgat gaacatggat gccctgaccg ccattgagaa ctttgccagc agtattttca
    16561 gtgccggaat ggatttcctg tttacctggg gagaattcat cggggtgatc agcatgatta
    16621 ccctgtttgc ccgcgcccgt tcagccggtc cggtaaaaat gtctcccgga aaatttattg
    16681 cggggatgtt aaccagctgc atgctcgtat cactcccggc aatgattaac gccggaggcg
    16741 tacaaatggg attcagggct gactcatttg gcccgattgc atatgtacaa cctcagacgt
    16801 ttggcgctgc tgctggcgca gcaaatgcag ttctgtcgct ggccaagctg gccggtgttg
    16861 gatttgtaat gaacgggata tccatctggc gcaaggccgg actcgatgga cataccgcac
    16921 tgagtgccag tgaatccgtc agtaagggaa acgtcaaatt tattgccggc gtcctgctgg
    16981 tctttatcga tcgtgtcctc aatgccctgc tggcaagcat tggaatcgtc ttctgatttt
    17041 tttcaccttc atataaacca ctgatatatc catttaagga gaaactatgt cacgtattcg
    17101 tgtcgtagcg gcccgtctgt acgccgcatt tatccacgca caactgtttg taatgtcatg
    17161 ccggagcaaa ctgactaagt tcctgctgct cattcccatg atgttgctgc ccaaagccgt
    17221 tcttgccgat ggagatcttg ccgatatggt tcgcaatgtc gaacaaggtg ccaaaacagc
    17281 acagtcatca tcactgacta ttgcgcagtt catcggcgta attctgtttc tcgggggcct
    17341 gattggcctg aaaaaagttg ggaaacaagg ggggatggga ctggctggtt gcattgtatc
    17401 tatcgtcatt ggggcgctac ttgtcgccgg acctgaaatg atgagtcgca gccagaaaca
    17461 gctggggatt tccacaattt ccattggcta atcttgtcca gggcactggc atttatccgg
    17521 tgcccttcct gttcggcaat acaaggaggc acatatgtct gcctggagcc cggagcacat
    17581 aacgttacga cgaacagaaa acggcaatgt gattgcccac gacagtcggg acgatcagga
    17641 attcattttt gcagaatgct gttccgatga aatcgcaaac atcattctgg aagccgtgaa
    17701 acgttacacc agtacggagc ctgctcatgc aacacagcat taaagacttg tggttgtatc
    17761 cctttccaga aattgatgtg gttcatacac aggagccact gttaccggaa ccggaattaa
    17821 caacgcccgg tcgatgtatc tgctgccggc aaaacgtcag gcaccgtttc agactggatg
    17881 actcatggcc actcagacag cttaccgaca caatcagtga cacacgagtt cgtcttaata
    17941 aggccacaga acatctggat aagttaaaaa agcgtggtga accagtcgca accggcgaaa
    18001 aggaaaagta caacacggca gtaaaagccg ctgaacgggc gcttgagcag gcccgtctgt
    18061 ctgccagacg tctttcactc cgccatgtac agaaagctga aatcaccagt acagagtcgt
    18121 tgtcagagaa agagcaggaa ctgtttcatg aagacggccc gccttacagc ttatgtgctt
    18181 tctgccacgc ctggcattca ctgaatggtt atgccgcagc acagggcgtt atggtgtggc
    18241 tacccgacct tcatccatcc acggtcgtgg cccttaaccg tcgttcatta caggaagttt
    18301 tcagtaatga taagttccgg gtccggcggg gacgggaagc gctctctgca ctgatgcaaa
    18361 acagactggc ggttgaggat aaattccgca gtttccgccc ggccgatttc gctgacgttt
    18421 ttcgtcgcta tccaccttcc ggtcgttcgc ccctgcgtga aaaaatgaac gggattgccc
    18481 tgattctgac accagattca ttcatcaaaa aagaatacgt tgattgattt ttcttcatgc
    18541 cactccagcg cggggtggca ttaaaaaaat caaactgcac aaacctccct cctgtaagtc
    18601 cggagttccc tcacctatga acataaaccg tttaatcttc actatcgaag actgtctgaa
    18661 cacgctatcc cgtttctctg tcgcaagcag ttttgttgag tattgcgatt tacgtactgt
    18721 aataggtctg gacagacagg atcgtgaacg tcgcccatgg ctgaattcgc cctatattgc
    18781 agccacaaag cgtggagaat atctctctgt ctttgaagtc agcggtgctt tcagggaaat
    18841 ggatgaagca tccgaccaga ctggcccagg ttcactggaa tcgctgatca ccagcatgag
    18901 tgacagcctg aatacggcat ataaaaacag cggtcataaa atcagcttcg tttttgagcg
    18961 tgatcctgac atggggaaag aagaaatcga agacatggtc gccccacaaa agcgctcact
    19021 ggctaataca ggtatccagt tacaggatgt ggtcgatgag aaagtcacaa cactgtcccc
    19081 atggctggtc cgggagcgtt gctggctggc gatctggagt ggacccgacc ttatcagcaa
    19141 cagcgatcgc accgcccatg atgagcttgt cagaagactg gctgaacggg taccgaaagc
    19201 acgctttgcc cagtccccct ggcagtggac cttatctgca ctgaaaatcc gccacgaagc
    19261 ttttcttgat aatgtggaac aggcactgag acattcatct gatggcctga ttctccggct
    19321 actggatata cacgaggtcg ggcgtgaaat acgtcgtcag acagaacggc acagcacacc
    19381 gcgtaactgg caaccccacc tgcctgaaga tgcccagcca gcgggatatc gctggactga
    19441 cgatgaatcc gtactacatg ctccttcatt gcatttgcag ttattcaaca ctcaggtgac
    19501 cacacagggc aacctggttc aggcgggtgg actctggcac ggaatggtat cgattacttt
    19561 acctccgcaa aatctgcaga cgtttaacga actggtccgt gccgtccccc gcgcagtccc
    19621 ctggcgcatc agaatggatc tgatgcccgg cgggatgaag gctcttaatc tgaagaaaac
    19681 gcttctgact tacagcagct tcatttctgc cgttcgtcca atgtatgaat ccgttatgac
    19741 cctggcggca acggatgaaa aagaacctgt ctgcataatg accatcatgg cctccacgtg
    19801 gggaaaaaca cgtgaaatat gcacacgaaa ccaggccatt ctgaaatcgg ccattgaagg
    19861 ctggggcgta tgtggcacca cgacaacatt tggcgatccc cgacgcgcct gggtgaatac
    19921 cattctcgcg gcatccggag gctccggccc ggttcccctg tatcccccgt tgtcacatgc
    19981 catctcgctt ttcccgctta acagggcagg ctctgtctgg cgaggcaaag ggaatctgat
    20041 gttacataca gaagacggtt ccgcatttga ggttggtctg gcgtcatccc aacaaaataa
    20101 acatacagaa ctggcaccag gcgatccggg actgggtaaa tcagttctga ttaatacgtt
    20161 gtcggaaata cagatatcat ctgctcagaa aaacctgccc ttcattgcct atatcgataa
    20221 gggatacagt gctcagggac tggttcagct tattcgcgac agtcttcctc aggaaagaaa
    20281 agacgaagct gtcggtatta ttctgagcaa tgatccggac tacacccgta acctttttga
    20341 cgtgatgtac ggcgctaaaa agcctattac gccggaaaag aatttcatgt catcggtgct
    20401 gtgtgctctc tgtgtcgata ccggtaccgg tcaaccctgt aaccccggag acacccgaca
    20461 aattatcaac cagcttatcg agctggcttt taaagagtac ggtgaaaata atccgcgact
    20521 ttatcgcgcc tcaacggaag aactggtgga ttcagcactg caggactcgg gactgtatga
    20581 aaaacatgat gcagcctggt gggccagatc aacctggttt gaagtccggg atatgctcca
    20641 caatgccggg tatatcatgg cggctcagcg ggcacattat caggcaatgc cccagcttcc
    20701 cgaagtgtcc tcgatgctgg gccataccag tctcagggat gttttcggta ctgttcagcg
    20761 tgatggttca aacgagcttc tgctggacta catccgccgc gccctggagc agggacataa
    20821 cgactacccc atgatatccg ggtatacccg gtttatgatc aacccggaaa cccgggtcat
    20881 cgctgtcgat ctgaataatg ttgccggtga caaaacgcct gcaggcaggc ttaaaaccgg
    20941 catcatgtac ctgcttgcag gtcagatcgc cggtggtgac ttcaccctgc cacaatacag
    21001 ggatgaagtg ttaaagcagc ttccacggga ataccatgaa atcgctctta aacgtattaa
    21061 ccaacttgat caggaggtta agacaaaagt ttatgatgaa ctgcataacg cccggggcat
    21121 tgacttcatc tgggaaaatc tggataccca ggagcgtgag caacgcaagt ttgctatccg
    21181 cacggttctg agtacgcagt atctgaggga ctacccggaa agcgttctta agtcagcaaa
    21241 tactctctgg ttactgcgct acaaacccga agatatcccc gtactgaggg ataacttcaa
    21301 cgtgcctgaa tttatgctga aacgcttcct gaaaatgccg gaaggcccgg caccagatgg
    21361 tagtggtgtt cctgttctgg gagtattcag ggtaaaaagt ggcacgcttg cgagaatact
    21421 caaatttacc gtcggtccac tggagctatg ggcgctgaac tcatcgccaa aagacagtgc
    21481 cctgcgtaaa acactgacaa ataagttagg ttcagttcgg gcacgtaaga ttctggccga
    21541 aaacttcccc agaggttctg caacgtcatt aattgagcac cgggcgggcc aacataacag
    21601 tgacaatgtt attgaagaac tggcctcgga gctcatccga aaacagggat acaatctctg
    21661 atgtgtacaa ttcatatcac cccacgctgc cggagacctg ccagagggca ggtatttctt
    21721 aaaaaacttg aggtaaaagg tgacagtttc tcattcactc tgccgtcacg tgtcttcagg
    21781 ttacatccgg cccccaatcc cagagtgata tacgcctcgg atgccagaac acagggaaag
    21841 gttacaggca tgcggctgtt gttgccgtcc agcacagatg agctgataac aacggcaata
    21901 tggcaggttt ctgtcccgga tgaagcacca ttcgacgttc agcatgtcat ccagtggata
    21961 ctcccttcac cgctggcttc gctgatgtcc gatgatacct ggttatggcc atccgttccc
    22021 ggaacgccgg cactgagtgc cggtgactgg cctgtaatgg atactgaccc ttccacctta
    22081 cggcgcgtca gacgtcgttc tttcagcatc atcgataacc ggaacaggaa aggttatatc
    22141 accaggcgat atcagtgttt cccactggcc ccactacctg aagacagaaa atacgacagc
    22201 cttcctgacc ttgtgctcca ccccgagcag gagacaataa aatgcaaaga aaaacattac
    22261 tcgcagccct tatagctatc ctgaatggaa cggcatacca ggctcatgct tattcagtca
    22321 ccgtcgtcgc cagccgtccc gttgaacagc aggtcgtccc cagaatggag gccatcaaag
    22381 gtgtgctgag tgacatactg agtacccaga cagccacggg taccgctatc aatcagaact
    22441 ctgaaaagct ggcaagtgtc attgcccaga acggacaggc gacacgtcag cagatgattt
    22501 tcagtaatga aactcagcgc ctcgaggagg cacggaaaag cttcaccgtg ccagactcca
    22561 tctgcagtga atccgcatca gggattgcaa cagaaagcaa aagtgcctca gcatccgcag
    22621 cctcaaaact cagtaaaggt ggcggtgtga gcaaccgtag catccgcgac cgtctggcca
    22681 gcgcagcaaa ctctcccgtg cgggaagctt atgatggcgc ggccatacac gccagttact
    22741 gtacagaagc agaatatgca cgttttggag gaaccgccgt ttgtccttct gtgggcgaaa
    22801 taccaggtgg agacagccag gtacgctcga tttaccacgg cgcaggtaca gcggacacac
    22861 ctgcggcact gacatgggat cagaagcaaa tagatgcagc gactgcttac atgaaaaaca
    22921 cttcccgccc ctctgccggt cgtgctctgg gtaaaggaga agtgaatacc cagagtgggc
    22981 gaacctacgt cggactccag aacgaataca acggcatcat tgattctgcg tcgaatcccc
    23041 aactgacgct gattgctgac agcacgccta atgaaagcac acgtaaagca ctggctgaaa
    23101 cactgcagtc agactcagcg gccgcttact tcgaccaggt ggcttcacca gaagcaaaag
    23161 cacggggata tatgagtacc cgtgagtttg aagcttttga ggctggccgg cgctacgcca
    23221 acacagcata cctggtggat ttacaggaaa tgcaggggga taacctgctc cgtgaactgg
    23281 taagaatcac tgcgcaaatg aactggcagc tgaacgatct gaaagaacag atccgccagg
    23341 gtaatgttat ctctggccag caactggctc tgactgcccg ccagtattat gaaaaacaac
    23401 tcggtagcct tgagaaaaca atcaatcagg caaacgcacg ttgaaagctg acgtcagaac
    23461 aggactgaaa aaatgaataa gttaccggaa aattcagacc agacgaaaaa cgtcaaaaaa
    23521 tcactatcca taacccgcat agccacccgg ggaattatta acatttttgt tcctctgatg
    23581 gagactcgcc tgataacgaa ttctgtacag catatgctgg aacaacaaaa acgcaggctg
    23641 gacagatttc gtcaggtaaa taataaaaag gcgcaactcc acctcacgtg ggaagaagct
    23701 ctgcaggcca gccatatgtc agtagacgat ctggatcgtc gtttccgccg gcgcagaaca
    23761 gtatggcgat tctgttgctg gagtctgctg gccatcgctt tgtttctcag tggtatgctc
    23821 tttgccgcct cttcattacc tcttacgact ctggtacgcg ccatcagcac tcttgtgttg
    23881 atactgtcag gcgtcgctct ttgtgcttcc agagcactga ttgttacata ccgcctctgg
    23941 cagttgcatg aacgaaaagt cagcgaaccc gagcagggga ctttccgtga ttttctgaac
    24001 gaccgcaatg gctggcgcaa tgccacatta attgccgtaa ccagtaaaca atactgatca
    24061 cgtccagatg ccagtaccac tggcatgtcg ccatccccct taattaaatc ctgtgtggcc
    24121 tttgcccata cgcaattcta ttccggagtt actgtgaaaa ttttacttcg cgcactctgt
    24181 gcaggccttg ctatatcctc actgccggca atggcttcag tgacctatca ggatattgtg
    24241 tctgccgcaa ccaatcctga cgatctgtcc cgtcaggcgc tggtcacgat attcggtgat
    24301 gtagtcacca atccactgtc aaccagcgcc cccactctga tcggaagcat gttcggggcg
    24361 ttcaacagta tcatcgccgt acttgccgtt gtctggttta tgttcattgg tatccgtcat
    24421 gttgtgcgtt cggggcatca gggacaggta ttcagcaccg gacgcgatat cgtcggcaca
    24481 ctgagtgtcg ttgcaggttt tctgatgatc gttccaaccg gaaatggctg gagtatcgct
    24541 cagctcatta tgttatgggg cgcatcgata atgggggttg ggtccgccaa tgtgatggtg
    24601 caacttgccg ctgataatat cgcaaatggt tattcaatga ctgtacagcc agttcaggca
    24661 tccacacgaa cagcagcgcg tggcattttt gaaatggaac tgtgtaagta tgcggttaac
    24721 gcaggactaa atgattttaa tcagacggtc aaatcctcga cttcactgat gacagaatca
    24781 gctaaaaccg ccagtggtaa ttacactgtc acggtatcca atggcagcgg tatctgtggt
    24841 tcagccagcc tgtcagtaga aggaaacgga actacagatc aatcgacgat tggaaaattc
    24901 tttaatccct tcagtaaaaa cgaatacagc ggtgttatct cagcacaacg tgcagccatg
    24961 gataatatga ttagcgacat ggacaacgct gccagtgagt ttgtgacaac atttctggaa
    25021 aaaagaaatt ctggcaacgg aacattgccg gatatcgaga cacggattca gagagcagcc
    25081 gacgagtatg aacgcgctgt acaaaaatcg ctacctattg ataacggaga acaatccagg
    25141 aaagaagctc tcaagtctta tctcacaacc tatggatggg tgacgcttgg cgcgtggtat
    25201 cagacctttg ccactgctaa ccagcgtctg gcagagctgg ctgacagagc tccagctgta
    25261 acttcaatgt catcacttgg ggaagttggc gatactgatt tatttagtgc tgttatgggg
    25321 gcatacaaga ctcaattaca gaactccaca tatactccaa cgataggaac gataacaact
    25381 caagatgaaa gtaatgcagc taatagcaca gacccacaaa gtgtggtcat gaaaagtata
    25441 gcgccaacca tattaaaatg gacaaaccaa gctgctactg aatggtcagg aacaggaaca
    25501 accagcaacc aagttaaccc attaattaag atgaaaaaca tcggcgatta tacattgggt
    25561 acaacagaaa ttctctggac aggatataca actgtcagag ttctcgcgac aatgggtgat
    25621 aactctattt tcggaaaggc tgttaatttg ttctctggat tacctaaagg atttgttgct
    25681 ctgctggatg ctgcagctcc cccaatatat ttcttgttgt tcctgttgtt ttgtgcgggt
    25741 ttttccttat caatttacct accatttata ccattcattt tctggatgac aggcattggg
    25801 aactggatag taagcgtatt aataggatgc accgccgggc ctctgtgggc agcaacacat
    25861 ctaggaacat cagaggacag aggaagtcga gcagcttacg gatatatata cttaattgac
    25921 agcatgataa gaccacctct catggtattc ggtttctttt ttgcctctgt cgccatcatt
    25981 gccgtcggca caatattaaa ctcactgttt ggcgcagcac tagttaacgt acaggtaaaa
    26041 tcattaacag ggatattcag ccttgccggg ttcctgctaa tttacgcccg tatgtgtaca
    26101 acgtctgtag ctgcaatatt tgctttacaa gcatatcttc ctgaccatgt tatcaatttc
    26161 ttgggtggta gagatggtgt aaatactctt ggcaacctca cctcatcagt aaaagatatt
    26221 tttgcaggtt ctaatagaaa tattagacac agccctggag ttagagagga cagactaaaa
    26281 aatgtcacaa aggatgataa taaagatgga ataaaaggat aattttaata aaacaacctt
    26341 gccataaaca ctcaatcgca ttaaaatatc gatgagtgtt tatggagggt ttattatgca
    26401 aatggtaaaa acaatcaata gatatcctgc ttggtggaca ttatattacg cccttagatt
    26461 tatatatttc ataagcatac cagtgttatc catttttatt cttttcggag tgctctccat
    26521 tacatcttca agatatgtga cgcaagaaga ttatatttat acttgcgtct gtctgttttt
    26581 actaattgcc cctgcaatat tgatgtattc ccgagcaagc tcaagaaaag acaaaataaa
    26641 aaaaattgtc gcggagataa aaaacaccgg tttttattca cctgacaaag aatatgaggg
    26701 actgtctttt actcagggcg tctattttgg cgtggatacc aaaaaaggca ctatgctgta
    26761 tgctcgtgct taccctggaa atataatgga tattatcggc tttgacattg acaacttcac
    26821 acgcactgtg accgacgcca agacactaga gatttataca aaatacataa atataccaat
    26881 ggtctcaatt ccgtcaggat gcatccatcc caaaatgatg gcggatacaa tgcatgcaat
    26941 ggcagaaaga ggatatgatt accctgtcga ctttcctcgc ctaattcagg agaagcgtaa
    27001 ggagtgggag cagattgcag gcatgcctgt agccgaagta ttttaagata agctccctcc
    27061 ataacagccg gttcaaatga gccggctttt gttcattaca acagataact tatgccgttg
    27121 ccgataaatt cgaccaagtg ctttaatcgc agcaattttt atttccatat catcactttg
    27181 actcaatcta tgtaattcac gagtaatgtt ctccgcctga caccggcctt cacctaatgc
    27241 atagatcgca gcaagtgtaa cgtcttttga tttactcttc atcatttcaa acaagaaagt
    27301 attagctatc gcaggaggca tgctcataat caatcctctt catgaaataa acaatggcac
    27361 tcacttacaa acaggagtcc actatcgcaa gcgactcaac ttagcaatat ctctcaattt
    27421 gaatgaaaaa accagatggc atgtgacagg gaaccgtccg catgatgtcc gtgaccattt
    27481 aaccccataa gctaaccttg tcacaaacgg gcgcagaaca gcctgccaga cagacaacaa
    27541 aacaaaagtc caagataaaa catgaatacc ggcatctcaa tgcgaagaaa taatctccgt
    27601 agaccccccc atgagaaaaa tatcatatgg gagaaaatcg caattatcac agccagcgca
    27661 ttcattattt tcgctgtgct ctggcattaa ttattttcaa cacaaaaagg aaaaaacaat
    27721 gaatctggta gacgcctttg tcaaaaaagt aatatccgag ccatatgaag aatatggaaa
    27781 atggtggata gatgttgaat acatttcatg gggtgttccc gggaaaacaa gactgatgtt
    27841 tgagtcaaag gagcaggcac tggaagttaa agaagggtac aaatttttaa cgtgagatct
    27901 ctttttgtta aagggcagga agcggtaact ccctgccctt agttacgttt ccaggcttat
    27961 cagtcgacaa gttcccggat cagttccaga acagcaatca gcgcgtttgc aacggcaact
    28021 actgccctga gaacgatgat taacagatta atcatccgct tctccatcac tggaggaaat
    28081 caccgttgct tactcttaca ttgcctgtca caacggcact ttcagtgtta gttgaaaaga
    28141 gacttcctcc tgccagagca ctgaacacag caccacctgt ttgttcagcc aggacttaaa
    28201 atcttgcatc catagtcccg atggatatca cggcagaagt gtcgccagac agagctaacc
    28261 tgcggattat aaggatggtt gctatcaaat gaacagctcg ctattataag aacgttagtt
    28321 gaaaagagat ttcctccggt cagggccacc cactctgtaa cggaacagta aaaaaaccga
    28381 cctcaccagt cggttttttt acatctgttg tttctcaaaa tccgcccgac atgcccgtca
    28441 caccgaggaa actcatgctc tgaatcagaa ccaatcatct caactttgcc gtctcctgaa
    28501 aaaactggca cattcagttc gataactacc cataaataca gagagaaaat ccgatgtcag
    28561 aaaagtggag taaagaattc gactcttgct ttcaggtggg gagcatagaa gaaattgccg
    28621 atgctctcga gaaacgcagc ggaagcgagg aaaatgcgtt agccatgctg gcaatgacag
    28681 ccttcacact catgacccga cgtggtatct gcgaaatgca gaacgtggcc cccgatggca
    28741 aaggcattcg gctggagcta atcggcttca gggaaaatga aacaataccg gatacgcttc
    28801 attgaagaag aaagggcgca atgaggtcag ccttcgcaac aaagcggcgg cagaaaacct
    28861 gccgccgtgt tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt
    28921 ttccctgctg gagccggagt ccgcaaagcg aatccctcac catccagaca aaacacagaa
    28981 tcgtcacaca gatgacgatt aacatcatta aaaacgttcg ctgtggcatc atagcccctc
    29041 cattgcctta cggcggttat cgggctacta taatgttgtc taggcattac agtagagccc
    29101 ttggttgatt gataaaaaaa tctccagggg ctttctgctg tataccgtcc acaaaacgct
    29161 ttcggcatac agcctcaagc gcccgcagct attctaacga ctttcgtatc tgcaacaatc
    29221 cttttatcgc acggctaatt cctctggtta gctcttatcg cctgcaaata aatttttaac
    29281 ctttccgttt gatttaaatt ccagaaatgg agactttgtc atgtcttaca atcgacagcc
    29341 tgtcgcagaa gatcctatgc agatatgggg cgctgttggt gtccttctta tcctgctgct
    29401 attcgtcata tggttattct tgccggaagt ggtttacgcc agctgcctga ttcttcatac
    29461 cttatggggg ctggtcgact gggggccatt tcataactat gcagcaccac gatacaacct
    29521 gcttgccatg accggtaaca acgccgccaa cataagctat agccagtggg ttaacgtcat
    29581 ggagcagacc ataggtattc tgtggatgta cctcttacct gtgacactct ggtgtttgtg
    29641 ggagtggtat caacatccgg gacaaagccg tttcacccgc agaccagtag atattacccg
    29701 actgccacac atctttgctt cgttgtcccc tgctatcgcc cctgtactgg cagatggtga
    29761 tccggaaaaa ctctttcatg gtggcaaacg acctgaacgg cgggttgctc tcaccccgga
    29821 agcatttgtc gaacagcata ctctcatcac caatatgcag ctcgatgttg cagctgcaag
    29881 acgatgtttc atggcccagt tgggtaaacc tttaacgtcc tggaaagaca tggcaccaca
    29941 tgaaaaagcg ctctttgcca tatttggcct tcagtatttc ctggatgatc gtaaggcagc
    30001 attaaagctc atggatacac tgaacctctc atgcagaatt aaaagcaaac gggacagcgg
    30061 gaaattctgt acccctgttt attcactggc caaatcagct ttccagagag tgattaaaag
    30121 caacggagca cagcaatggc tgaaacaaca tcgctacgtc cgcagtgggc tggtctggct
    30181 gtatgcacat gacctgcggc ttacgccacc caactggatt tggctgaaag gcgtggacag
    30241 aacgctgttt tatgcattac accgcgccaa taccacgaaa ggatttattg aaggcgctgg
    30301 tgtggtggcc gttgcacgtg cagaggccga agccatgcgt tttggccttc cctgccctga
    30361 accctgtgtt gatgaagcgg tggaaggctt acggcgggat atgctcagtc tcggtcttat
    30421 ctgggatgaa ccacaaccag atcgcgaccg gaaacgccgc atcctgacca actggtccct
    30481 caccgacgac atcctgcccc gcacacctgc tacagacaac gaattctgat tatccggaga
    30541 actgaaaatg acaatcgagt actttgcaac acgcattgca aaacatattt ccgtgcaggc
    30601 aatctggccg gatggtaaga cagaaattat tgccgtcctt ccacgggatg ccctgtcagg
    30661 gctggtaaca cgaaataatc aactgattca tatagccttt gacgccatgg gcacccgtaa
    30721 cagagaaacc gtcctgattg ataaacagga gcataacgct gaccagatac ttacagctat
    30781 tgattcctgc ctgcgccttg aatatctgat ggaacgccgc ttcagcagaa cacctttgtt
    30841 ccgtggcata atcgccagcg ttgtgctgtt cgtaatggca acgattggct tctctctgtt
    30901 ccgctatggt gatcgtgtat tctgggatga tacaacgcct gaagcagtac agacagcagg
    30961 tgagccacga ctgcttcccc ctcacctgaa tcacactgtg ccattaaatg aaggcattca
    31021 actcccggtt cccccgaaaa aagacgtcca ggtcgcggag aaaacaatca caagcaaaaa
    31081 tccggaagcc gcagctgcgc gacataatct ggctactgtt ctgaagcgga acgccgatcg
    31141 gggcatgttt accatcaacc tgtcatccgg tcatgaacga accctgttcg catttctcga
    31201 tcctgcctgt cctaactgca gacttctgga gccggcctta aaacgcctgg ccagcgactt
    31261 caatgtcgtt atctacccgg tttcagttat tggtggagag gaatccaccg accgtgtggc
    31321 accactgctg tgtgaaaaag atgctcagaa acgtgctgcc ggctggcacc gactgtattc
    31381 cgcagataac ggaatgatga cgccatcaga agaaacaacg cctgcagatg aaacatgcct
    31441 gaaagcagcc agagctgcga ttgatgttaa caatgtcgcc ttccgtaagt tcggatttgc
    31501 cggtacccca tgggtgctgt ccgacacggg ctggcatctg cctactggaa ttctgcagga
    31561 aactggcacg cttaatttat tcctgaaaac caccgacagc gagtctggcc atgagtgaac
    31621 atcgcgttaa ccccgagctt ttacaccgta cagcctgggg aaatcctgtc tggaatgcac
    31681 tccagagtct caatatctat ggcttttgcc tggtggcaag tcttgtggcc agttttatct
    31741 ggccacttgc attaccggca tgtctgttgt tcacactgat aacgatgctg gtatttagtc
    31801 tgcagcgatg gcgctgccct cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac
    31861 aggacagaat gattaaaaga agccttttca gcttctggcc aactctgttt cagtatgagg
    31921 tcatcctgga gtcaccggcc agcggcatat tttatgttgg ttatcaacgc gtcagggata
    31981 taggtcggga gttgtggctg agtatggacg atctgacccg ccatataatg tttttcgcca
    32041 ccaccggtgg tggtaaaacg gaaaccatct ttgcctgggc gattaacccg ctttgctggg
    32101 caagaggatt cacccttgtc gatggcaagg cacagaatga tacggcaaga acgatctggt
    32161 atctggccag acgctttggc cgtgaagacg atgtggaagt catcaacttc atgaacggag
    32221 gaaaatcccg cagtgaaatc attctgagtg gtgaaaaaac acgacctcag tcaaatacct
    32281 ggaatccctt ctgttacagc actgaggcat tcacagcgga gacaatgcag tcaatgttgc
    32341 cccagaacgt ccaggggggc gagtggcagt cccgggccat tgccatgaac aaggcactgg
    32401 tatttggtac taaattctgg tgtgtgcgcg aaggcaaaac aatgtcgctg cagatgctcc
    32461 gtgaacatat gactctcgaa gggatggcca aactttactg tcgtggcctc gatgatcagt
    32521 ggccggaaga agctatcgca ccgttacgta actaccttca ggacgttccc ggtttcgacc
    32581 tgtcgctggt caggacacca tcagcctgga cggaagagcc cagaaaacaa catgcttatc
    32641 tctccgggca gttttctgaa actttcagca cattcacgga agcattcggg gatatttttg
    32701 ctgaagattc cggtgatatc gatattcgtg acagcattca cagcgacaga attctgatgg
    32761 taatgatccc cgctctggat acatcagcgc atacaacgtc cgctctggga cgaatgttta
    32821 tcacccagaa aagtatgatt ctcgcccgtg atttgggcta ccgcctcgaa ggaactgatt
    32881 ccgatgcact ggaagtcaaa aaatataagg ggcgtttccc ttatctgtgt ttccttgatg
    32941 aagtgggggc atactataca gaccgtattg ctgtggaagc cactcaggtg cgttcactgg
    33001 attttgctct gattttgatg gcacaggacc aggaacgtat agaaggacag acaacagcca
    33061 cgaatacggc aacactgatg cagaacacgg ggacaaaatt tgccggcagg attgttagcg
    33121 agggtagtac ggccagaacg ctgaaaagcg ccgccggtga agaagccaga gcgagaatga
    33181 acaacctgca gcgtcaggac ggcatatttg gtgaatcatg gattgacagc ccacaaatca
    33241 gcatcctgat ggaaagcaag ataaatgttc aggagttgat tgaacttcat cctggcgagt
    33301 tcttttctat tttccgggga gaaaccgtac catcagcctc attcttcatc cctgacgatg
    33361 agaaaagctg cagcagcgat cccgttgtta taaacaggta tatcagcgtg gatgcgccac
    33421 gcctggaccg tctccgccgt ctggttcccc gtactgctca acgtcgaatc ccgtcaccgg
    33481 aaaatgtcag tgccatcatt ggcgtcctga ctgcaaaacc ctccagaaaa agacgaaaaa
    33541 ttcgcacaga gccgcacacg atcgtggata cattccagca acgcattgca ggacgtcagg
    33601 cggctatggc aatgctggaa gagtacgaca cggatatcaa cgcacgtgaa agtgccttat
    33661 gggaaacagc ggtgaatacg cttaaaacga ccacccggga agagcgacga attcgctata
    33721 tcacgctgaa cagaccagaa ctacctgaaa caaatgagga aaatcagata tcagtgagag
    33781 cagaaagagc cagtataaac ctgttaacgc ttccacagga caataaccac ccgacagggc
    33841 gtcctgtaaa cgggtttcat cataaaaaaa ccaaccgtcc ggactgggac ggaatgtact
    33901 gaactgtgac catacttcgg tcttcccgca aaaaacctat ttaaaaatgc tggtactaga
    33961 taccagcatt cagattacag attaaccgca tcattgtagg tacatatgga acgtctaatt
    34021 tttatattct ttaaccctag atcatctaat tttaaccgaa gctgttcatc agattgttca
    34081 aataaaggcc ccaacacaat ctctctaatt gccgtaatat tgttacaggt aaatttcacc
    34141 ttagtataat acgcaattcc gtaatcagta ctacgttttt caagtttgta tggtggggat
    34201 gtatgttcat ctacttcaat aagtcctcgg acctcatttt cacattcaaa actctctctc
    34261 ttatataatg aacatagtct gataagactt gcatgaagta tgccacgtgc acatttaata
    34321 tttaaacttg agcgattaat ataatcatgc aatgcttctc ttacatattc tcgaaacagt
    34381 tcttctgaat agataacagg acgaaaaaca actctaccac taataggttg aaaacgttct
    34441 tca
//
LOCUS       DAZPJP010000026        34131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPJP010000026 DAZPJP010000000
VERSION     DAZPJP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.026"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHU9J_001142"
     CDS             38..580
                     /locus_tag="ACHU9J_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1904741.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHU9J_001143"
     CDS             663..1364
                     /locus_tag="ACHU9J_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1904742.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHU9J_001144"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHU9J_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHU9J_001145"
     CDS             3979..5049
                     /locus_tag="ACHU9J_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1904743.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHU9J_001146"
     CDS             5061..5603
                     /locus_tag="ACHU9J_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1904744.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHU9J_001147"
     CDS             5611..6126
                     /locus_tag="ACHU9J_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1904745.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHU9J_001148"
     CDS             6092..6829
                     /locus_tag="ACHU9J_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1904746.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHU9J_001149"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHU9J_001149"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1904747.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHU9J_001150"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHU9J_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1904748.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHU9J_001151"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHU9J_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1904749.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHU9J_001152"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHU9J_001152"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1904750.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHU9J_001153"
     CDS             12136..14043
                     /locus_tag="ACHU9J_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1904751.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHU9J_001154"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHU9J_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1904752.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHU9J_001155"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHU9J_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1904753.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHU9J_001156"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHU9J_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1904754.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHU9J_001157"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHU9J_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1904755.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHU9J_001158"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHU9J_001158"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1904756.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHU9J_001159"
     CDS             complement(18711..20471)
                     /locus_tag="ACHU9J_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1904757.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHU9J_001160"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHU9J_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1904758.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHU9J_001161"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHU9J_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1904759.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHU9J_001162"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHU9J_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1904760.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHU9J_001163"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHU9J_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1904761.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHU9J_001164"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHU9J_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1904762.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHU9J_001165"
     CDS             complement(26072..26518)
                     /locus_tag="ACHU9J_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1904763.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHU9J_001166"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHU9J_001166"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1904764.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHU9J_001167"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHU9J_001167"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1904765.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHU9J_001168"
     CDS             complement(29281..29943)
                     /locus_tag="ACHU9J_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1904766.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHU9J_001169"
     CDS             30116..30529
                     /locus_tag="ACHU9J_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1904767.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHU9J_001170"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHU9J_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1904768.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHU9J_001171"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHU9J_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHU9J_001172"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHU9J_001172"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1904769.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHU9J_001173"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHU9J_001173"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1904770.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHU9J_001174"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHU9J_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1904771.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHU9J_001175"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHU9J_001175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8828 a         8131 c         8898 g         8274 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca a
//
LOCUS       DAZPJP010000027        32837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPJP010000027 DAZPJP010000000
VERSION     DAZPJP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.027"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHU9J_001176"
     CDS             267..1925
                     /locus_tag="ACHU9J_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1904772.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHU9J_001177"
     CDS             complement(1922..2878)
                     /locus_tag="ACHU9J_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1904773.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHU9J_001178"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHU9J_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1904774.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHU9J_001179"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHU9J_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1904775.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHU9J_001180"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHU9J_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1904776.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHU9J_001181"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHU9J_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1904777.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHU9J_001182"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHU9J_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1904778.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHU9J_001183"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHU9J_001183"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1904779.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHU9J_001184"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHU9J_001184"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHU9J_001185"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHU9J_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1904780.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHU9J_001186"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHU9J_001186"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1904781.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHU9J_001187"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHU9J_001187"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1904782.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHU9J_001188"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHU9J_001188"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1904783.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHU9J_001189"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHU9J_001189"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1904784.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16450..16944
                     /gene="lrp"
                     /locus_tag="ACHU9J_001190"
     CDS             16450..16944
                     /gene="lrp"
                     /locus_tag="ACHU9J_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1904785.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17079..21146
                     /gene="ftsK"
                     /locus_tag="ACHU9J_001191"
     CDS             17079..21146
                     /gene="ftsK"
                     /locus_tag="ACHU9J_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1904786.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21301..21912
                     /gene="lolA"
                     /locus_tag="ACHU9J_001192"
     CDS             21301..21912
                     /gene="lolA"
                     /locus_tag="ACHU9J_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1904787.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21923..23266
                     /gene="rarA"
                     /locus_tag="ACHU9J_001193"
                     /gene_synonym="mgsA"
     CDS             21923..23266
                     /gene="rarA"
                     /locus_tag="ACHU9J_001193"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1904788.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23357..24649
                     /gene="serS"
                     /locus_tag="ACHU9J_001194"
     CDS             23357..24649
                     /gene="serS"
                     /locus_tag="ACHU9J_001194"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1904789.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24888..27332
                     /gene="dmsA"
                     /locus_tag="ACHU9J_001195"
     CDS             24888..27332
                     /gene="dmsA"
                     /locus_tag="ACHU9J_001195"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR1904790.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27343..27960
                     /gene="dmsB"
                     /locus_tag="ACHU9J_001196"
     CDS             27343..27960
                     /gene="dmsB"
                     /locus_tag="ACHU9J_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1904791.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27962..28825
                     /gene="dmsC"
                     /locus_tag="ACHU9J_001197"
     CDS             27962..28825
                     /gene="dmsC"
                     /locus_tag="ACHU9J_001197"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1904792.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28861..29487)
                     /gene="ycaC"
                     /locus_tag="ACHU9J_001198"
     CDS             complement(28861..29487)
                     /gene="ycaC"
                     /locus_tag="ACHU9J_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1904793.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29802..30950
                     /locus_tag="ACHU9J_001199"
     CDS             29802..30950
                     /locus_tag="ACHU9J_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1904794.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31160..32590
                     /locus_tag="ACHU9J_001200"
     CDS             31160..32590
                     /locus_tag="ACHU9J_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1904795.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32591..>32837)
                     /locus_tag="ACHU9J_001201"
     CDS             complement(32591..>32837)
                     /locus_tag="ACHU9J_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1904796.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8032 c         8814 g         7934 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaacagta
    16201 ttcttatatg cgcataacca tgcatgtaaa taccatgttt accgtgctag tgaaatctac
    16261 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    16321 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    16381 tagcgactct gaacagtgat gtttcagggg cagacaggag tagggaagga atacagagag
    16441 acaataataa tggtagatag caagaagcgc cttggcaaag atctcgaccg tatcgatcgt
    16501 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    16561 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    16621 attcagggct atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc
    16681 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    16741 caaaaacttg aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg
    16801 aaaacacgcg tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt
    16861 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    16921 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    16981 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    17041 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    17101 acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga
    17161 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    17221 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    17281 gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    17341 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtccagcgac gaatacattg
    17401 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    17461 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    17521 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    17581 tctgcgtttg ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg
    17641 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    17701 atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa gatgaaaatc
    17761 acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta
    17821 aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct
    17881 ccggtaagcg gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg
    17941 accctgacga cgtcctattt tcgggcaatc gtgcaacgca gccagaatat gacgaatacg
    18001 atccattatt aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca
    18061 cggcggcaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    18121 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    18181 cgggagagcc ggttattgct cctgcaccgg aaggttaccc acagcagcca caatatgcgc
    18241 agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccacag cagccgtatt
    18301 atgcacctgc agctgaacaa cctgtgcaac aaccttacta cgctcctgcg gcggaacaac
    18361 ctgttcagca accgtattac gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc
    18421 aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact gagcaaactt
    18481 atcagcaacc agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc
    18541 ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccactttact
    18601 attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc
    18661 aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgctg aaagcacctt
    18721 ctgttgcagc agtacctcca gtagaaaccg ctgccgctgt ttctccgctg gcatctggcg
    18781 tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc
    18841 tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg cagttgccac
    18901 gaccgaaacg tatccgcgtg ccaactcgcc gtgaattggc gtcttacggt attaagctgc
    18961 cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca gcgcaatcag tacgattctg
    19021 gcgatcagta caacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt
    19081 tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtaaacg
    19141 cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg caaactcaac
    19201 aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg
    19261 aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgttg tttacgccaa
    19321 ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc gcagcagcag tatcagcagc
    19381 cacaacagcc ggttgcgcca cagcagcaat atcagcagcc gcaacagccg gttgcgccgc
    19441 agccacaata tcagcagccg caacaaccgg ttgcgccaca gcagcaatat cagcagccgc
    19501 aacaaccagt tgcgccgcag ccgcagtatc aacagccgca acaaccggtt gcgccgcagc
    19561 agcagtatca gcagccacaa cagccagttg cgccacaacc gcaggatacc ctgcttcatc
    19621 cgctgttgat gcgtaatggc gacagccgtc cgttgcataa accgacgacg ccgctgcctt
    19681 ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    19741 aacaaatggc tcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgttg
    19801 tcaattactc tccggggccg gttatcactc gctttgaatt gaacctggca ccgggcgtaa
    19861 aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg acggtggcgg
    19921 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta ccgaataaaa
    19981 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    20041 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    20101 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct gtcggtgtga
    20161 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    20221 tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcatctg ttaacggaag
    20281 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    20341 gtcgttataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    20401 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca
    20461 gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtggtg ttggttgacg
    20521 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg
    20581 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt ccatcggttg
    20641 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    20701 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctggggatgg
    20761 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    20821 ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    20881 atgttgatgg catcacttcc gacagtgaaa gcgaaggtgg cgcgggcggt ttcgatggcg
    20941 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    21001 cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    21061 tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    21121 tgctggctcc accgccgttt gactaattaa tgcattgccg gataaggcgc ggtagcgtcg
    21181 catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag ctgattatta
    21241 gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg aggaataatg
    21301 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg
    21361 gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc
    21421 ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca gggcgatctg
    21481 tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga aagcattctg
    21541 gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaact
    21601 tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc
    21661 gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
    21721 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag
    21781 tttagcgcgg tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat
    21841 ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat
    21901 caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataatacttt
    21961 tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg gccagcaaca
    22021 tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatc tgcattctat
    22081 gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga ttgcccgcta
    22141 tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag agattcgcga
    22201 ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga
    22261 cgaagtgcac cgattcaaca aaagccagca ggatgcattt ctgccacata ttgaagacgg
    22321 caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta attcggcact
    22381 gctttcccgt gcccgtgtct atctgttgaa atccctgggt acagaggata ttgagcaagt
    22441 actaactcag gcgatggaag acaagacccg tggttatggt ggtcaggata ttgttctgcc
    22501 agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa
    22561 tacgctggaa atgatggcgg atatggccga agtcaatgat agcggtaagc gggtcctgaa
    22621 gcctgaatta ttgaccgaaa tcgccggtga gcgaagcgcc cgctttgata acaaaggcga
    22681 tcgcttttac gatctcattt ccgcactgca taagtcggta cgtggtagcg cacccgatgc
    22741 ggcgctctac tggtatgcgc gaattattac cgctggtggc gatccgttat atgtcgcgcg
    22801 tcgttgcctg gcaattgctt ccgaagatgt cggcaatgcc gacccacggg cgatgcaggt
    22861 ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg aacgcgccat
    22921 tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt
    22981 taaagctgcg ctggcggatg ctcgcgaacg cccggattat gacgtgccgg ttcatttgcg
    23041 taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc gttacgctca
    23101 tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa tagcacaaac
    23161 acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa agctcgcctg
    23221 gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg
    23281 taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga
    23341 taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc
    23401 tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg ctcttgaaga
    23461 gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc
    23521 gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt
    23581 gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga
    23641 aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa
    23701 agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt ttgactttga
    23761 agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg cagctgcagt
    23821 taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc
    23881 actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt
    23941 tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg
    24001 cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat
    24061 cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg atgaagatga
    24121 tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg
    24181 tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat
    24241 cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt
    24301 cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg acatgggctt
    24361 tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca cctaccgtga
    24421 gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg cacgttgccg
    24481 cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt
    24541 tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc gtattgaagt
    24601 accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt
    24661 tctgaatcta aaaagcgcct gcgggcgctt tttttgtctc cctttgatac cgaacaataa
    24721 ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt
    24781 tcaaattatt cgatgtatac aagcctatat agcgaactgc tatagaaata attacacaat
    24841 acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa
    24901 tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga
    24961 tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg
    25021 ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag
    25081 ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg
    25141 tcgaaacgga caataccggc gatgacaatt acgacggcct gcaccaggtt cgcgcctgcc
    25201 tgcgtgggcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa tatccgatga
    25261 aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg
    25321 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag tctatctatc
    25381 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg ccgggaaata
    25441 ccctggtcgc gcggctgatg aactgctgcg gcggctatct gaaccattac ggcgactact
    25501 cctccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca gatggcaaca
    25561 gcccgtcgga tatcgaaaac agtaagctgg tagtgctgtt tggtaataac cctggcgaaa
    25621 cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    25681 cccgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    25741 ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg tacgtcatga
    25801 tcactgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tacgatgaga
    25861 aaaccctgcc agccagtgcg ccgaaaaatg gccactataa agcttatatt ctgggtgaag
    25921 ggccagatgg cgtggctaaa acaccggaat gggcctcaca aatcaccggt gttccggcag
    25981 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    26041 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    26101 cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt gaaggttcat
    26161 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    26221 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    26281 tacgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    26341 gcctggttaa ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga
    26401 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    26461 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcgctggat gcatcctgcg
    26521 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    26581 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    26641 aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    26701 ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    26761 aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac ccactgacta
    26821 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    26881 aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    26941 atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc
    27001 gcgttcactc aacttacggc aacgttgatg tgctgaaagc agcttgccgt caggaaatgt
    27061 ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct
    27121 ttaacgatcg tggtgaagtt catattgagg cgaaagtgac gccacgaatg atgccaggtg
    27181 tggtcgcact gggtgaaggt gcctggtatg acccggatgc aaaacgtgtc gataagggtg
    27241 gttgtattaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    27301 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    27361 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac
    27421 tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc tggcggcgac
    27481 tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct gtcgatttcg
    27541 tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa
    27601 cgtgaagatg gttttgttgt ggtcgatgaa gatgtgtgca ttggctgccg ctactgccat
    27661 atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat gaccaaatgc
    27721 gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga atcttgtccg
    27781 ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg cgatctggcg
    27841 gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat caaacccaat
    27901 gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa ggaggtgtga
    27961 gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg ggcaatgtgt
    28021 ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc gagcagaagc
    28081 ccagcagcgt gttatcgcct gcatgtttgg cttatgggtg ctgatgggca ttggctttat
    28141 cgcttctatg ctccatcttg gttcaccgat gcgcgctttt aactcgctca accgggtagg
    28201 ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgccg taggcggcat
    28261 cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc tttggctggt
    28321 ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat
    28381 tgataccgtt ccgacctggt acagcatctg gacgccgatg ggcttcttcc tgacgatgtt
    28441 catgggcggc ccgttactgg ggtacctgtt gctgagtctg gctggcgtcg atagctgggc
    28501 gatgcgtctg ctgccagcca tttccgtact ggcactggtg gtgagtggcg tggtgtcagt
    28561 gatgcagggc gcagagctgg cgactattca tagttctgtg cagcaggccg cagcgctggt
    28621 gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt
    28681 gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctactat ccgtctcatt
    28741 cattctgctt ctggcagggg aattaatcgg tcgcggcgta ttctatggtt tgcatatgac
    28801 cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc cggttctctt
    28861 ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat caggaatatg
    28921 gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacggt gcagctcgca
    28981 agccacgccg aaccatgtca tcaattgcgc acctgcttgc gacatgcgat cccatgccga
    29041 atgccgggta atttcattaa aggtccctga cgcgtcagtc acaacaaaca catcaaaccc
    29101 ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca caccggcaat
    29161 aattaactgt tttttacctg tcgctttgac tgcttttaca aaatcttcgt tatcccaggc
    29221 gttaatattg cccgggcgag caatgtaagg tgcatcgggg aattgtgctt tcagttctgg
    29281 aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg gtaagttgaa
    29341 atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg gttcgatatc
    29401 ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacagcag catcattttt
    29461 atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag tggtacgcct
    29521 gaaatcggtc agacgtgatg cattgctctg aaagcataga cgggaaatat gagtttgctg
    29581 tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat caataaaaac
    29641 aatcagatga gattaatcat ctcttcagta ttagcgttat gaatgagtaa tataaatcga
    29701 taataccgcc gttgcgatat gcgcgtattt tcacccattg acaatgtttt tggcggtggc
    29761 atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg tatacccggc
    29821 ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata
    29881 cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta ggcgttgtca
    29941 gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat gtcattaaac
    30001 gcattggttt taaccgcagc tattatctgg cctccttcat ttttgccgct ggctgtgccg
    30061 gccttggcct gatgattgga ttctggagtt ggttggcgtg gcgttttgtc gcgggcgtcg
    30121 gctgtgccat gatttgggta gtcgttgaga gcgcgctgat gtgcagtggg acgtcacgta
    30181 accgtgggcg tttgcttgct gcatacatga tggtttatta cgtgggaacg tttttaggcc
    30241 agttactggt cagcaaggtc tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt
    30301 tgacattggc agggatctta ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa
    30361 accatgattc gacgtcaatt acggcaatgc taaaactccg tcaggcgcgg cttggcgtga
    30421 atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg cctctgtacc
    30481 tcaatcacaa aggggtgagc aatgccagta ttggtttctg gatggcggta ctggtcagtg
    30541 cgggtatcct cggacaatgg ccgattggac gtctggcgga taagtttggt cgactgctgg
    30601 ttttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg
    30661 cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat ccggtggcga
    30721 tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac caagccttac
    30781 tgttgagcta tactgtggga agtctgcttg gcccgtcatt taccgctatg ctaatgcaga
    30841 atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc tatttgctga
    30901 tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa atgaattcaa
    30961 gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc tgacttgctt
    31021 tattccaaat tttattcgtt taaaaataaa atgtgtagca ggttataatt ttgcatttcg
    31081 ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg cccctataat
    31141 tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagctgc gatggtacaa
    31201 cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta ataactacgc
    31261 caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcgctct atttcacacc
    31321 ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg gcggggtcag
    31381 tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt ggacctactg
    31441 ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg cgctcggttg
    31501 ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt tacaggggtt
    31561 aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga aatcgctgaa
    31621 aatcgtcggt tctgtggcag ggattgctat gtttgttatg tcgctcctgt atgtggcgat
    31681 ggcggtaacc gcgcctgcaa ttactgaagt gcatattgcg accacaaaca ttacctggga
    31741 aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc tggttttcgc
    31801 ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc caggaaaaga
    31861 atttccaaaa gggatgttat gcctggcggt gatggttgcg gtttgtgcca ttctgggctc
    31921 gctggcgatg gggatgatgt ttgattcgcg taatatcccg gatgacttaa tgaccaacgg
    31981 tcagtattac gcctttcaga agctgggcga gtattacaac atgggtaata ctttaatggt
    32041 gatttacgcc attgcgaata ccctgggaca agtagcggcg ctggtattct cgattgatgc
    32101 cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca gtttatgtcg
    32161 taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtac tggtggcgat
    32221 tctgattatg ctgccgactc tcggcattgg tgatatgaac aatctctata aatggctgtt
    32281 gaaccttaat tcggtagtga tgccgctgcg ttatttatgg gtatttgttg catttattgc
    32341 agtcgttcgc ctggcgcaga aatataaacc agaatatgtc tttattcgta ataagcctct
    32401 ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgtt tgacggggat
    32461 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
    32521 aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa
    32581 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    32641 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttgggtaa tagatctgga
    32701 agcctggacg ctctgtgatc cagtcttcca gtacacattc taaacgaccg gtatcaaggt
    32761 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcatcca
    32821 gttcagcatc aacatca
//
LOCUS       DAZPJP010000028        31952 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPJP010000028 DAZPJP010000000
VERSION     DAZPJP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31952
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.028"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(138..1496)
                     /gene="plaP"
                     /locus_tag="ACHU9J_001202"
     CDS             complement(138..1496)
                     /gene="plaP"
                     /locus_tag="ACHU9J_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1904797.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1486..1548)
                     /gene="yoeI"
                     /locus_tag="ACHU9J_001203"
     CDS             complement(1486..1548)
                     /gene="yoeI"
                     /locus_tag="ACHU9J_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1904798.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1763..2692)
                     /locus_tag="ACHU9J_001204"
     CDS             complement(1763..2692)
                     /locus_tag="ACHU9J_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1904799.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2738..3562)
                     /locus_tag="ACHU9J_001205"
     CDS             complement(2738..3562)
                     /locus_tag="ACHU9J_001205"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1904800.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3645..3899)
                     /gene="yoeB"
                     /locus_tag="ACHU9J_001206"
     CDS             complement(3645..3899)
                     /gene="yoeB"
                     /locus_tag="ACHU9J_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1904801.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3896..4147)
                     /gene="yefM"
                     /locus_tag="ACHU9J_001207"
     CDS             complement(3896..4147)
                     /gene="yefM"
                     /locus_tag="ACHU9J_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1904802.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4430..4480
                     /gene="hisL"
                     /locus_tag="ACHU9J_001208"
     CDS             4430..4480
                     /gene="hisL"
                     /locus_tag="ACHU9J_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1904803.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4456..4579
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4626..5525
                     /gene="hisG"
                     /locus_tag="ACHU9J_001209"
     CDS             4626..5525
                     /gene="hisG"
                     /locus_tag="ACHU9J_001209"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1904804.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5531..6835
                     /gene="hisD"
                     /locus_tag="ACHU9J_001210"
     CDS             5531..6835
                     /gene="hisD"
                     /locus_tag="ACHU9J_001210"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1904805.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6832..7902
                     /gene="hisC"
                     /locus_tag="ACHU9J_001211"
     CDS             6832..7902
                     /gene="hisC"
                     /locus_tag="ACHU9J_001211"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1904806.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7902..8969
                     /gene="hisB"
                     /locus_tag="ACHU9J_001212"
     CDS             7902..8969
                     /gene="hisB"
                     /locus_tag="ACHU9J_001212"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1904807.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8969..9559
                     /gene="hisH"
                     /locus_tag="ACHU9J_001213"
     CDS             8969..9559
                     /gene="hisH"
                     /locus_tag="ACHU9J_001213"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1904808.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9559..10296
                     /gene="hisA"
                     /locus_tag="ACHU9J_001214"
     CDS             9559..10296
                     /gene="hisA"
                     /locus_tag="ACHU9J_001214"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1904809.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10278..11054
                     /gene="hisF"
                     /locus_tag="ACHU9J_001215"
     CDS             10278..11054
                     /gene="hisF"
                     /locus_tag="ACHU9J_001215"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1904810.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11048..11659
                     /gene="hisIE"
                     /locus_tag="ACHU9J_001216"
     CDS             11048..11659
                     /gene="hisIE"
                     /locus_tag="ACHU9J_001216"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1904811.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11756..12733)
                     /gene="wzzB"
                     /locus_tag="ACHU9J_001217"
     CDS             complement(11756..12733)
                     /gene="wzzB"
                     /locus_tag="ACHU9J_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1904812.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12879..14045)
                     /gene="ugd"
                     /locus_tag="ACHU9J_001218"
     CDS             complement(12879..14045)
                     /gene="ugd"
                     /locus_tag="ACHU9J_001218"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1904813.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILSSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14295..15701)
                     /gene="gndA"
                     /locus_tag="ACHU9J_001219"
     CDS             complement(14295..15701)
                     /gene="gndA"
                     /locus_tag="ACHU9J_001219"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1904814.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15865..17235)
                     /gene="cpsG"
                     /locus_tag="ACHU9J_001220"
                     /gene_synonym="manB"
     CDS             complement(15865..17235)
                     /gene="cpsG"
                     /locus_tag="ACHU9J_001220"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1904815.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(17340..18776)
                     /gene="cpsB"
                     /locus_tag="ACHU9J_001221"
     CDS             complement(17340..18776)
                     /gene="cpsB"
                     /locus_tag="ACHU9J_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1904816.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(18779..20002)
                     /gene="wcaI"
                     /locus_tag="ACHU9J_001222"
     CDS             complement(18779..20002)
                     /gene="wcaI"
                     /locus_tag="ACHU9J_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1904817.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(19999..20478)
                     /gene="gmm"
                     /locus_tag="ACHU9J_001223"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(19999..20478)
                     /gene="gmm"
                     /locus_tag="ACHU9J_001223"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1904818.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(20481..21446)
                     /gene="fcl"
                     /locus_tag="ACHU9J_001224"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(20481..21446)
                     /gene="fcl"
                     /locus_tag="ACHU9J_001224"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1904819.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(21449..22570)
                     /gene="gmd"
                     /locus_tag="ACHU9J_001225"
     CDS             complement(21449..22570)
                     /gene="gmd"
                     /locus_tag="ACHU9J_001225"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1904820.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(22596..23144)
                     /gene="wcaF"
                     /locus_tag="ACHU9J_001226"
     CDS             complement(22596..23144)
                     /gene="wcaF"
                     /locus_tag="ACHU9J_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1904821.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(23160..23906)
                     /gene="wcaE"
                     /locus_tag="ACHU9J_001227"
     CDS             complement(23160..23906)
                     /gene="wcaE"
                     /locus_tag="ACHU9J_001227"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1904822.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(23917..25134)
                     /gene="wcaD"
                     /locus_tag="ACHU9J_001228"
     CDS             complement(23917..25134)
                     /gene="wcaD"
                     /locus_tag="ACHU9J_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1904823.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(25109..26326)
                     /gene="wcaC"
                     /locus_tag="ACHU9J_001229"
     CDS             complement(25109..26326)
                     /gene="wcaC"
                     /locus_tag="ACHU9J_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1904824.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(26323..26811)
                     /gene="wcaB"
                     /locus_tag="ACHU9J_001230"
     CDS             complement(26323..26811)
                     /gene="wcaB"
                     /locus_tag="ACHU9J_001230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1904825.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(26814..27653)
                     /gene="wcaA"
                     /locus_tag="ACHU9J_001231"
     CDS             complement(26814..27653)
                     /gene="wcaA"
                     /locus_tag="ACHU9J_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1904826.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(27746..29908)
                     /gene="wzc"
                     /locus_tag="ACHU9J_001232"
     CDS             complement(27746..29908)
                     /gene="wzc"
                     /locus_tag="ACHU9J_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1904827.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(29911..30354)
                     /gene="wzb"
                     /locus_tag="ACHU9J_001233"
     CDS             complement(29911..30354)
                     /gene="wzb"
                     /locus_tag="ACHU9J_001233"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1904828.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(30360..31499)
                     /gene="wza"
                     /locus_tag="ACHU9J_001234"
     CDS             complement(30360..31499)
                     /gene="wza"
                     /locus_tag="ACHU9J_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1904829.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         7738 a         8433 c         8008 g         7773 t
ORIGIN      
        1 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
       61 tgaggatttc gataaaaaaa ataaaaaaac cggagccaaa tattcattcg actccggttg
      121 attattagaa aagatgatta tgcaacgtct tcgtactgcg gaactgggtt acggaagctc
      181 ttggtgacgc aagccaggta aatcagaccg atagcagccc aaatcagacc cagaaccatt
      241 gagctttctt ccaggttaac ccacagcgca ccaacggtca gcgcaccaca catcggcagg
      301 aacagatact ggaagtgatc tttcagcgtc ttattacgct tctcacggat ccagaactgc
      361 gagatgaccg acaggttaac gaaggtgaac gccaccaacg caccaaagtt aatcagcgct
      421 gtagccatta ccaggtcgaa gttgattgcc agcagggcaa tcgcgccaac caggatgatg
      481 ttcatcgccg gagtacgcca tttcgggtgt acataaccga agaagctttt cgggaatacg
      541 ccgtcacgac ccattacgta catcagacgc gctacgcctg catgcgccgc cataccggac
      601 gccagtacgg taatggtgga gaagatcagc gcgccaacct ggaacgcttt acccgcaacg
      661 tacagcatga tttcaggctg tgacgcatcc ggatctttaa agcgagagat atccgggaag
      721 tacagctgca ggaagtaagt tgcaaagatg aagatcatgc cgccaatcag cgcggtcagg
      781 aaaatcgcac gcgggatcac gcgctctgca tctttggttt cttccgacag gttgctgatg
      841 ccgtcaaagc cggtaaagga aaagcacagg attgtcgccc cggtaatcat cggaataaca
      901 tgcgcatcgc cagaccagaa cggacgagtg ctcgccagcg taccagcgcc ttcaccttca
      961 aatacgccat aaacaaccat gcccagaatc accgcgatca gcactacctg caacacgacg
     1021 attacagtgt tgaagttcgc tacggatttc agactacgca ggttaaaggc ggtcataaag
     1081 gccaccagcg ccaccacaaa catccacgat gggatggaag gcaccagagc ttcaaaatag
     1141 attttcgcca gcagaatgtt gatcatcggc gcgaacagat agtcgagcag agaagaccaa
     1201 cccaccataa agccgacagt cgggctaatg gatttctggg cgtaagtata tgcagagcca
     1261 gcagaaggat agcggcgaac cagcttcccg tagctcagag ccgtaaacag gatcgcaatc
     1321 aatgcgaacg cataggctgt cggcacatga ccatccgtga ggcctgaaac gataccaaaa
     1381 gtatcaaaca gcgtcatcgg ctgcatatag gcaagaccca tcattacaac cggaactaac
     1441 gtaagcgttt tacgcaattc cacgcgagag gtgtttggag taacgttatg cgacatggtc
     1501 attctccttt acggtgataa ccgtcgcgta agcaaaaaat tgccccattt ttttggattc
     1561 ctcagcgaca acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct
     1621 tggtatgaat gattggtttg aagcaaaaaa taaccgacgc tgatgaaacg tcggttttta
     1681 gtcatttttt gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga
     1741 agcttgtcat aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa
     1801 caccggcgat cgcactattt gctaaaattt catcccgcca ccagccgctg gcaagccctg
     1861 cggtctgttc gttccagcca atccagacgg gttcaaagag agtttgggcg accactttct
     1921 tctcgattaa cgcgccacta tcgagaaaac gttgcaccag ataacggggt aaatagccgc
     1981 agccgaggcc gctaatttgc agctccagct tggttttaaa atcgaaaacg gtaatggctt
     2041 cttgttcgtc gagaagttgt gaggccgtag aagcgcctgc ctgcgcagtg tcccccacca
     2101 caatcgcccg atatcgctta atgatacgac ggtttaacgg ctcttcttcc tgggctaacg
     2161 gatgatgagg cgcgaccgca aagacttgtt ccagatcgcc caaccgcgag aaaccaaact
     2221 cactggaaga tggtggctca tgcatcgcgc caacaataat atccgctcgc ccctgagtca
     2281 gggcatccca ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat
     2341 gttgataaaa agcttcaatc agtggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc
     2401 caatcaccag ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc
     2461 gcacggtatg cagaacttcc cgccccttct ctaataacat ttttccggta cgggtgaatt
     2521 tagcgcggtg accgctacga tcaagtaatt gaatattaag gtcgctttcc agcttgtgaa
     2581 cggtgtaact cagagcggac ggcgtcttgt agagtttagc cgacgccgcc gcaaaactgc
     2641 cttctttttc taaggcatcg aggatcatca acacatccag cagttgtttc atacacgccc
     2701 ccttgcagtg catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg
     2761 gatcgggata ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct
     2821 tacctttgcc gctgtccaga ctatttctga actgcggagg ttccagcccc agtaaacggg
     2881 ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt
     2941 gtccgccttt aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga
     3001 ctaaattaac accatgttca ccatcaggcg cggtttttcc ggcaaagaag cgtccgggat
     3061 gacgtcccgg tccgaccagc cccgcaagac gcagaatatc gaccgaagta ccgggtaaat
     3121 tgtgcagcca gtcttcgagt tcttctaata ctcgtccact gttggtcacc ggattacgcg
     3181 gggtggtttc tttcaccgtg ccttgcgcgt cgccatagac agacgttgag ctggtaaaaa
     3241 taatgcgagg aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat
     3301 agaactcatc gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat
     3361 ccatcagggc atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat
     3421 agctatcaat gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg
     3481 tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg catgcctaac caccctaacc
     3541 cgacaattgc gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac
     3601 gctagcgtat caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga
     3661 catgctgcaa tgagcagtga atcgtcggta accgcgtata ccagacggtg ctcctctgta
     3721 atgcgtcggg accagaaacc tgacaaatta tgtttcaggg gttctggctt ccccttacct
     3781 tcaaatggcg ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta
     3841 tctgtttcct gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
     3901 caataatgtc cttttctgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
     3961 tccgggcgtt agcgggggag cgcagtagat aagccgtctc ttccagcgag ttgtattctt
     4021 cgagtgacat cagaacacaa gcctctccat tctgacgagt aataaggatc ggggcatgat
     4081 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
     4141 tacgcatgtc aatctcctct tttgtacagt ttattgtaca atgatgggcg ttaattaact
     4201 atttattaat tagtttgtag atcaaggtat tgtcagtgag acgaaaatcc aggcttcgct
     4261 atttttggtg ccgtcagctt agaggacagt cctcttagcc ccctcctttc cccgctcatt
     4321 cattaaacaa atccattgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
     4381 tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta tgacacgcgt
     4441 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
     4501 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
     4561 cttccggggg cttttttatt gcgcggttga taacggttca gacaggttta aagaggaata
     4621 acaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
     4681 atgactcacg cgaattactg gcgcgctgtg gcattaaaat taatcttcac acccagcgcc
     4741 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gacgacattc
     4801 ccggtctggt aatggatggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
     4861 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ttgcgtcgtc
     4921 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggcc
     4981 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     5041 tcgaccagaa aggcatctct tttaaatcct gcttactgaa cggttctgtt gaagtcgccc
     5101 cgcgtgccgg actggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     5161 caaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctt atccagcgcg
     5221 atggtgaaat ggaagaatcc aaacagcagc tgatcgacaa actgctgacc cgtattcagg
     5281 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgtctgg
     5341 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     5401 accaacaacg cgtagcgatg cacatggtca gtagcgaaac cctgttctgg gaaacgatgg
     5461 aaaaactgaa agcgctgggt gccagttcaa ttctggtcct gccgattgag aagatgatgg
     5521 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     5581 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcactgttaa
     5641 cgatattctc gataacgtga aagcacgcgg tgatgacgcc ctgcgggaat acagcgcgaa
     5701 gtttgataaa accacggtta acgcgctgaa ggtgtctgct gaggagattg ccgccgctag
     5761 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaaa atattgaaac
     5821 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     5881 ccagcaggtc acgcgcccgg tcgcttcagt tgggttgtat attcctggcg gctccgcccc
     5941 gctcttctca acggtattaa tgctggcgac tccggcgcgt attgcgggct gtaaaaaagt
     6001 ggtgctgtgc tcaccgccgc cgattgccga tgagatcctt tatgcggcgc agctgtgcgg
     6061 tgtgaaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     6121 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     6181 gaaacgtcag gtgagccagc gtctggacgg tgcggctatc gatatgcccg caggcccgtc
     6241 ggaagtgttg gtcattgccg acagcggcgc aacgccggat ttcgtggctt ctgatttgct
     6301 ttctcaggct gaacacggcc cggactcaca ggtgatttta ctgacgcctg acgctgatat
     6361 ggcgcatcaa gttgccgaag ccgtcgaacg ccagttagca gaactgccgc gtgccgaaac
     6421 cgcccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     6481 agagatctcc aaccagtacg gcccggagca cctgatcatc cagacccgca acgcccgcga
     6541 tctggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     6601 ggcaggtgat tacgcctccg gcaccaacca cgttctgccg acttacggtt acaccgccac
     6661 ctgttccagc ctcgggctgg cagatttcca gaagcgtatg accgtgcagg aactgtcgaa
     6721 agaaggtttc tccgcgctgg cttcgaccat tgaaacattg gccgccgccg agcgcctgac
     6781 cgcccataaa aatgccgtta ctttgcgtgt taacgccctt aaggagcaag catgagcacc
     6841 gtgactatta ccgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     6901 cgtcgtctgg gcggtaatgg cgacgtctgg ctgaacgcca acgaataccc cactgccgtg
     6961 gagtttcagc ttactcagca aacgctcaac cgctacccgg aatgtcagcc gaaagcggtg
     7021 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagtcgtggc
     7081 gcggacgaag gtattgaact gctgattcgc gctttttgcg aaccgggtaa agacgccatc
     7141 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ccgaaacgat tggcgtcgag
     7201 tgccgcacag tgccgacgca ggacaactgg caactggact tacagggcat ttccgacaag
     7261 ctggacggcg taaaagtggt ctatgtttgc agccccaaca acccgacagg gcaactgatc
     7321 aatccgcagg attttcgcac tctgctggag ctaacccgcg gaaaagccat tgtggttgcc
     7381 gatgaagcct atatcgagtt ttgcccgcag gcatcgctgg ctggctggct gacggaatat
     7441 ccgcacctgg ctattttgcg cacactgtcg aaagcttttg ctctggcggg ccttcgttgc
     7501 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat cgccccctac
     7561 ccgctctcga cgccggttgc cgacattgcg gcccaggcgt taagcccgca gggaatcgtc
     7621 gctatgcgcg aacgagtgac gcaaattatt gcagaacgcg aatacctgat tgccgcactg
     7681 aaagagatcc cctgcgtaga gcaggttttc gactccgcaa ccaactacat tctggcgcgc
     7741 tttaaagcct ccagcgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     7801 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     7861 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     7921 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     7981 ttgacaaact cgcctttgaa ccaggcgtga tcccggaact gctgaagctg caaaaagcgg
     8041 gctacaaact ggtaatgatc accaatcagg atggtctggg aacacaaagt ttcccgcagg
     8101 cggatttcga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtacagt
     8161 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     8221 aagtaaaact ggtggaacgt tatctggctg agcaagcgat ggatcgcgct aacagttatg
     8281 tgattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     8341 gctacgaccg cgaaatcctg aactggccaa tgattggcga gcagctcact aaacgagacc
     8401 gttacgctca cgtagtgcgc aacaccaaag agacacagat tgacgttcag gtgtggctgg
     8461 atcgcgaagg tggcagcaag attaacaccg gtgttggctt ctttgatcac atgctggatc
     8521 agatcgccac ccacggtggt ttccgtatgg aaatcaacgt caaaggcgac ctctatatcg
     8581 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaatggctc
     8641 ttggcgacaa acgcggtatt tgccgctttg gttttgtgct accgatggac gaatgccttg
     8701 cccgctgcgc gctggatatc tctggtcgcc cgcacctgga atacaaagcc gagtttacct
     8761 accagcgcgt gggcgatctc agcaccgaga tgatcgagca cttcttccgt tcgctctcat
     8821 acaccatggg cgtgacgcta cacctgaaaa ccaaaggtaa aaacgatcac caccgtgtag
     8881 agagcctgtt caaagccttt ggtcgcaccc tgcgccaagc catccgcgtg gaaggcgaca
     8941 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ccggctgcgc
     9001 caatctgaac tcggtgaagt cagccattgc gcgccacggt tatgaaccaa aagtcagccg
     9061 tgacccggac gtggtgttgc tggccgataa actattttta cccggcgttg gcactgcgca
     9121 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     9181 accggtactg ggcatctgct tagggatgca actgctgggt cggcgcagcg aagagagcaa
     9241 cggcgtcgac ttgctgggca tcattgacga agacgtgccg aaaatgaccg actttggtct
     9301 gccgctgccg catatgggct ggaatcgcgt ttacccgcag gcaggcaacc gcctgtttca
     9361 ggggattgaa gacggcgcgt acttttactt tgttcacagt tacgcaatgc cggtcaatcc
     9421 gtggaccatt gcccaatgta attacggcga accgttcacc gcggcggtac aaaaagataa
     9481 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     9541 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     9601 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     9661 ttacaggatt acgccgcgca gggtgccgaa gtgctgcatc tggtggatct gaccggggca
     9721 aaagatccgg caaaacgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtt
     9781 ccggtgcagg ttggcggcgg tgtgcgtagc gaagaagacg tggcggcgtt actggaagcg
     9841 ggcgttgcgc gcgtggtggt tggctccacc gcggtgaaat caccagaaag ggtgaaaggc
     9901 tggtttgaac gtttcggtgc cgatgcctta gtgctggcgc tggatgtccg tattgacgag
     9961 caaggcaaca agcaggtggc ggtcagcggc tggcaagaga actcgggcgt ttcactggaa
    10021 caactggtgg aaacctatct gcctgtcggc ctgaaacatg tgctgtgtac cgatatctcg
    10081 cgcgacggca cgctggcagg ctctaacgtc tctttatatg aagaagtgtg cgccagatac
    10141 ccgcaggtgg catttcagtc ctccggcggt attggcgaca ttaacgatgt tgctgccctg
    10201 cgaggcactg gcgtgcgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
    10261 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc atgtctcgac gttcgtgatg
    10321 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat tattggcgat attgtaccgc
    10381 tggcaaaacg ctacgccgaa gaaggtgcag acgaactggt gttctacgat atcaccgctt
    10441 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
    10501 ttccgttctg cgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
    10561 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaacc ttaattactc
    10621 gcctggcgga tcgctttggc gtgcaatgca ttgtggtcgg cattgatacc tggtacgacg
    10681 gcgaaaccgg taaatatcat gtgaatcaat ataccggcga tgaaagccgc acccgcgtca
    10741 ctcagtggga aacactcgac tgggtacagg aagtgcaaaa acgtggagcc ggagaaatcg
    10801 tcctcaacat gatgaatcag gacggcgtgc gtaacggtta cgatctcgaa caactgaaaa
    10861 aagtgcgtga agtttgccac gtcccgctaa ttgcctccgg tggcgcgggc accatggaac
    10921 acttcctcga agccttccgc gatgccgacg ttgacggcgc gctggctgct tccgtattcc
    10981 acaaacaaat aatcaatatt ggtgaattaa aagcgtacct ggcaacacag ggcgtggaga
    11041 tcaggatatg ttaacagaac aacaacgccg cgaactggac tgggaaaaaa ccgacggact
    11101 aatgccggta attgtgcaac acgcggtatc cggcgaagtg ttaatgctgg gctatatgaa
    11161 cccggaagcc ttagacaaaa ccatcgaaag cggcaaagtc accttcttct cgcgcactaa
    11221 acagcgactg tggaccaaag gcgaaacgtc gggcaatttc ctcaacgtag taaatattgc
    11281 gccggactgc gacaacgaca cgctactggt actggcgaat cctatcggac caacctgcca
    11341 caaaggcacc agcagctgct tcggcgacac cgctcaccaa tggctgttcc tgtatcaact
    11401 ggaacaactg ctcgccgaac gcaaatctgc cgacccggaa acctcctata ccgccaaact
    11461 gtatgccagc ggcaccaaac gcattgcgca gaaagtgggc gaagaaggcg tagaaaccgc
    11521 actggcagcg acggtacatg accgctttga gctgaccaac gaggcatcgg acttgatgta
    11581 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacggtaa ttgagaacct
    11641 gcgtaaacgg catcagtgag ttgcagggta agcggatgcg atgttgttgt cgcatccggc
    11701 aaaaaaaaag ggcaaggtgt caccaccctg cccgttttct ttaaagctgc aaagattact
    11761 tcgcgttata attacgcaga gcgttacgcc ccagcacaat ccccgcgcca accatgccgc
    11821 ccagcagcac cgccagaatc aaggtaattg cctttttcgg gctatcgcga cgaataggta
    11881 acgtcggttt catcacataa cggtaagcat gaatatcaag atcatcaacc tttaagtttt
    11941 caatatccaa caggttttga cgtgtctgat agtagtttgg tgagaacacc aacggacggg
    12001 tcgcctcatg cttaatcatc gactccagcg cttcgctccc caaaaggaac aacgtatctt
    12061 gtgtcacatc ttcagtctgc tgaacctgtg gctttgccac ctgcgcctga ttcgcatact
    12121 gcaacgcttc ctgaatctga cggatacgca gatctttctg ctcctgcgcg actacttcct
    12181 gcgttcttaa agagtcctgc aagtttttcc gtcccagagc aatgttgtct ttgagatcct
    12241 tttctagctc ttgattcact ttatcatcaa cttgctgaat gtattgggcc aacttcattt
    12301 gtgctccctc tgcagtttgc ccaacataag aaacagtcaa tggtaattgc tggttcttaa
    12361 cagaaggttc gatggtaagt ttttctggtt cttcctgatt atccagcgtt tctgctaatg
    12421 cagagaaggc agaactgaag cgaccaatta acgtctcctg taaatccgat actttcggtg
    12481 cagcctgacc atagataaca ttcatggcat tgttatagcc agcaatttgc cccacgtcgg
    12541 gctgagtgat aattgctgtt gacgtccatt tctccttcgc tactgccaaa tatccaatag
    12601 ccagggcaat agccacaatg acggaaatga tgattgtcat cttgccacgc cacaactgca
    12661 ctagtaaatc aatcaaatca atctgttccg ggtcatggtt ttgcccagaa acattattat
    12721 tttctactct catcattacc ctaactgacg gaaatttcca agtccgcata gtttagcgat
    12781 aatcctagtg aatgctatag gataagtgat aaaaataatg aattattggg gaggtaacaa
    12841 aagacaatca gggcgtaaat agccctgata acaagatgtt agtcgctgcc aaagagatcg
    12901 cgggtgtata ccttatctgc cacatcttta agctcttctg ccattcggtt agagataatg
    12961 acgtcggctt gttgtttgaa gatggcgaga tcacgttcca gccgagagtt gaagaatgag
    13021 tcttctttca tcactggctc gtagatgatc acttcaacgc ctttcgcctt gatacgcttc
    13081 ataatcccct gaatagaaga cgcacggaag ttatctgaac cgctcttcat aatcagacga
    13141 taaataccca ccacttgcgg tttgcgtgac aaaatggcat cggcaataaa atccttacgc
    13201 gtgcggtttg catcgacaat tgccgagatc aggttattcg gcacagactg atagttcgcc
    13261 agtaactgct tggtatcttt cggcaagcaa taaccaccat aaccaaacga cgggttgttg
    13321 taatggttgc caatacgcgg atcgagacaa acgccttcga taatctggcg agtattcaga
    13381 cctaaacttt ctgcgtagct atccagttcg ttaaagtatg ccacgcgcat cgctaggtag
    13441 gtgtttgcaa aaagtttaat cgcttctgct tcggtcgaat cggtgaacag ggtcgggata
    13501 ttttgcttaa tcgcgccttc ctgtaacaac gcagcaaaac gttctgcgcg ttctgaacgc
    13561 tcaccgatga caatacgtga aggatggaga ttatcgtaaa gggctttacc ttcacggaga
    13621 aattccggag agaaaataat attttcagta cgatatttct tatgcatcgc ttcggtaaaa
    13681 ccaacgggaa ccgttgattt gatgaccata accgcataag gatttatctc aactacgtct
    13741 ttaattactg attctacact ggatgtattg aaataattag ttttaggatc atagtcggtt
    13801 ggagtggcga tgatgacata atcagcatcc cggtaggctt catttttatc taatgtggca
    13861 ttaaagtgta ttttatctga ttgcaaaaac tgctgaattt ccttatcaac aataggagat
    13921 atccgatcat tcagcatagc aacgcgtgac gataaaatat ctaatgccac aacctcatga
    13981 ttttgtgcga ttagaagccc gtttgacaag cctacatagc cagtaccgga aatggtgatt
    14041 ttcatttcat gctctcagaa ttaacttaac tgtgaatcat gatgttttta gcatcctgat
    14101 aagagctaaa tgttttaacg ctacgttatt agctggtcaa cgctacgaaa aacaattcag
    14161 attttcttta taagaatagc tgatatttat taagttaata ttaagcagtg aaatttagtc
    14221 tataagataa atggcaaaaa aaaagcccgg tgcaatatac gccgggcctc aattttactg
    14281 ttggttaaat cagattaatc cagccattca gtatggaaca caccttcttt atcaatgcgc
    14341 ttataagtat gtgcaccgaa atagtcacgc tgtgcctgga tcaggttcgc aggcagaaca
    14401 gcggcacggt agctatcgta ataggcaacc gcagcggcga aggtcggaac cgggataccg
    14461 ttctgtactg cataagcaac gacatcacgc agcgcctgct ggtagtcatc ggcaatttgc
    14521 ttgaagtacg gagccagcag caggttagcg atctgcggat tttcggcata tgcatcggtg
    14581 attttctgca ggaactgcgc acggatgatg cagccagcac ggaaaatctt cgcgatttcg
    14641 ccgtagttca gatcccagtt gtattcttca gacgcagcac gcagctgaga gaagccctga
    14701 gcgtaagaaa cgattttgcc cagatacagc gcacggcgaa ctttttcgat gaactcagcc
    14761 ttgtcgcctg ctgactgagc ttgcggacca gagagaactt tagatgcggc aacgcgctga
    14821 tctttcagag aagagatata acgtgcaaac acagactcgg taatcagcga cagcggttct
    14881 ccgagatcca gcgcgctctg gctggtccat ttaccggtac ctttgttagc cgcttcatcc
    14941 aggatcacat caaccaggta gttaccgtct tcatcttttt tagtgaagat atctttggtg
    15001 atgtcgatca ggtagctgct cagttcaccg ttattccact cggtaaaggt ctgtgccagt
    15061 tcttcgttag agagattcag gccgcctttc aggagagaat aggcttcagc aatcagttgc
    15121 atatcaccgt attcaatacc gttgtgaacc atcttcacat aatggcctgc gccatcagca
    15181 ccgatatagg taacgcacgg ttcgccatct tcagcaacgg cggcgatttt ggtcaggatc
    15241 ggcgcaacca gttcataggc ttctttctgg ccaccaggca tgatggacgg ccctttcagc
    15301 gccccctctt ctccgccgga aacaccggta ccgatgaagt taaagccttc ggcagaaagc
    15361 tcacggttac gacgaatggt gtcctggaag aaggtgttac caccatcgat gatgatgtcg
    15421 cctttatcga gatatggttt gagcgaatca atagcagcat ccgtgcctgc acctgctttc
    15481 accattaaca ggatgcgacg aggcgtttcc agagattcaa caaactcttt caccgtatag
    15541 taaggaacca gtttcttgcc tggattttcg gcaatcactt cttccgtctt ttcacgggaa
    15601 cggttgaaaa tagagacggt ataaccacgg ctttcgatgt tgagcgcaag gttgcgcccc
    15661 atcactgcca taccgactac gccgatctgt tgctttgaca ttgtttactc ctgtcagggg
    15721 gtggtcaccg ccgagtgtgc ggcttaaatg tggtatagat gttaactcac tcatagagat
    15781 ggtgaatagc attacctttg ctataccaca ctgaatatct caccttttcc gtcttttaaa
    15841 acttatttat atgaaattac tgaattactt gttcagtaac tcaagaataa gttttgtctt
    15901 ttcttccatc agcggcacat caccgcgaga ttccacattc aaccgcacca ccggttcggt
    15961 gttggaggtg cgcaggttaa agcgccagtc ggcaaaggtc atgctgatgc catcggtgcg
    16021 atccaccgcc agcgcctcgc ggctaaaatg ctgttccacg cggttaatcg cctcaacggg
    16081 ttgcgccagt ttgctgttga tctcaccgct tgccggaaac gctgccatcc ggtcgcgcac
    16141 cagttcgccc agcgtttttc ctttcaggca caccagttcg gcgaccagca gccacgggat
    16201 catgccgctg tcgcagtaag cgaaatcacg gaagtaatgg tgggcgctca tttcaccacc
    16261 gtagatggcg tcttccttgc gcatccgttc tttaataaag gcgtgtccgg ttttcgacat
    16321 caccggcgtg ccgcctgcgg cagtcaccac atcaacggtg ttccaggaga gacgcggatc
    16381 gtggatgatc ttcgcgccgg gatttttttc gaggaatgct tctgccagca ggccgacaat
    16441 gtagtagccc tcaataaact gccctttttc gtcaaacagg aagcagcggt caaaatcgcc
    16501 atcaaaagca atgcccatat ccgcaccgtg tttgatgacc gcattgcggg tgtcgtcgcg
    16561 gcattccggc agtagcgggt taggaatacc gttggggaaa ttgccgtccg gcgtgttgtg
    16621 cactttgatt aattccacgg gtgcgccaag ggctttaaag cgggcttcga tggcgtccac
    16681 caccggaccc gctgcgccat tcccggagtt aatcaccagc ttgagcggcg tgaggttttt
    16741 gacattgata taaccgaaca ggtgatcaac gtaagcgtca cgcagattga tttgctgata
    16801 gcgaccgcgt ttggtttcat caacgggagg aaagtcgttg gcttctgcca gacgctggac
    16861 atcgcgcagt ccggtatcac cgctgatcgg gcgagcccct tcgcgcacca gtttcatgcc
    16921 gttgtaatcc atcgggttat ggctggcggt aacttcaatg ccgccatcca cgccgagatg
    16981 gaacgtggcg aaatagatct cttcggtgcc ggacatgcca atatccagca catcgacgcc
    17041 cgcatcctgt aaacctctcg ccagcgccag ttttaaggtt tcgctggtga ggcggacatc
    17101 accgcctaac acaatggttt tcggtttgag aaattcgcca taggcgcgac caatgcgcca
    17161 ggcgatatct tcattcagtt cttcgcctaa tttcccgcga atatcatagg ctttaaagca
    17221 ggttaatttt ttcatgtcgt tacccttttt caggcaatag ttggccctga ccgaaacggc
    17281 caattttgtt tttgtcgtta tttacatctc ggtttcgcat tcattgcctg atgcgacgtt
    17341 tacacccgtc cgtagcgatc cgcgaaacgc accacatcat cctcttcgag ataagagccg
    17401 gagcgcactt caattaaatc gagcggaatt ttccccgggt tttccaggca atgcatcgcc
    17461 cccagcggaa tataaatgga ctcgttttca ccaagcagtt tgatatcacc atcaatggtg
    17521 acttttgccg ttcccgcgac aaccacccag tgttccgcgc ggtgatggtg catctgtacc
    17581 gacaagccct cgcccggttt cacggtgatg cgtttcacct ggtagcggtc gcccgcgtcg
    17641 atagagtcat atttgcccca cgggcgatag acttcgcgat gcacccgatg ctcatggcga
    17701 ccatcggctt tgatctgctc gaccactttt ttcacatcct gcaccgcgtt acggtcggca
    17761 atcagcaccg catctttggt ctgcaccact accagatctt tcaccccgac ggtggttacg
    17821 aggccagatt cggcgtacac atagctgttt tcagttttgt gattaatcac atcgccgtgg
    17881 caaacgttgc cctcggcggt gtgggcgcta atttcccata atgaggacca ggagccgaca
    17941 tcgctccagc ccgcatccat cggcaccaca acggcatctg ccgtacgttc catgaccgcg
    18001 taatccaccg actcttccgg gcaggcgaga aacgcttctt catccacacg aataaaatcg
    18061 agatccggat cgacggcgct catcgctttt tcacaggcat cgagaatatc cggacgatat
    18121 tttttcagtt cttcgagata gcgtccggcg cggaacagga acatgccgct gttccagtaa
    18181 tattcgccgc ttgccacata ggcctgggcg gtttccagat tcggtttttc gacaaactgc
    18241 gccacttcaa aggccaccgt atcctgctca cccgcagaca cttcaccgcg acgaatatag
    18301 ccataaccgg tttctggtag atccggcaca atgccgaagg tcaccagctt gcccgcttcg
    18361 gcatatggca tggcattacg cacggcggca cggaacgcgt cttcatcggc aatcacatga
    18421 tccgccgcca ataccagcat taacgggtcg ctctccgggc tatgacgttt tgccgccagc
    18481 gccgccagcg caatggcagg tgccgtgttt cgccctgccg gttcgagaat aatgttctcg
    18541 gtaagtttgt tcagttgacg cagctgttcc gcgacaataa agcggtgctg ctcattgcaa
    18601 atcaccaccg ggctttcgca ctccacgccg ttcaggcggc agatggtggt ttgcagcatg
    18661 gtgagatcgc ctttcaggca taaaaactgc ttggggtaaa gtacgcggga aagcggccat
    18721 aagcggctac cggagccacc tgccatcaca actggataga gtttcgactg cgccataatt
    18781 atccccgaat atcatttata aattgacgta acacgttctc tttatcgagt gtgcgttcgg
    18841 catattcacg tgccaccgtg ttgtgtttgg gcagcaggag cgcctgacgg atccccgcca
    18901 ccagcgcctc gaccgattcc ggttctacgc aaaccgcaat gcccggaaag gtttcgcaaa
    18961 gctgccccag ttctgtgtgg gcctctgcgg taatcaccgc gttaccgcct accgccagaa
    19021 tattggtcag tttcgacggc aatacggcat ctgccgcgcc gcgtttttgc accaccagat
    19081 ggcaatcgcc catcttcagc agtgcgggta aagcgtcata cgattgcagc gggaaaaatt
    19141 gcatgttgcg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg ccgccgccct
    19201 gcccgacaat ggcaaaaatc agcggttcat cgcgcagacg atcggcagct tcaataacgt
    19261 tttccagccc ctgcttttca ccaatattgc cggagtaaag aatgattttt ttgttatccg
    19321 gcaggtcaag ctggttacga agggcgtcaa catcggcatc tgcaacatgc tgaaaacggg
    19381 caatttccga ccagttgggg aagaagatga cgttttccgc cgccacgcct ttttcgatgg
    19441 ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc agtccgctac
    19501 gttcgaacgc cgttgccagc tgtgccactt tgctgccttt gccttttccg gcaaggccca
    19561 gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc gcaccagaaa
    19621 gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggcaccacg ccgataatgc
    19681 gatccggctt ccagcgacgt tgcgccatca gaggaaagaa actgctgacg gcaaaactgc
    19741 cgagatgtaa caggcgtttc agggtgctcg gctgttttgg cacgtacagc gggcagcgcc
    19801 acaccgtggc ggccccctct tctcgtttgt accgccaggc ggaatagttc tcgcccacct
    19861 gccactgcgg gtagtaaggc ggtgcggtaa taacccgcac ctcatgacct tgtgccgcca
    19921 gccattccac catctcgccg gtgtatttgc cgataccggt taactccggc gagtagttaa
    19981 tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag cgagaaaata
    20041 ggcgcggctg ttagcgtgaa catcgttact ggcgagcagc gcgtccggcg tcagccagcg
    20101 gtaatcgtca tgctgctcat ccggcagtat cagctcttct tcagcgactc tgaagcgaaa
    20161 accgagcacc acatagtgag tggtgaaatc cgtgccggag aagttatcgt cataaaagtg
    20221 ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca gttccgccat
    20281 cgtcagccgc tcgaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc ctcccggcac
    20341 aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca agcagaaact caccgcgact
    20401 gttctcgaca ataaagtcga gagagacaag cggggtggag cgcactaccg tggcaaagtc
    20461 ttcctgacgt aaaaacatca ttacccccga aagcggtctt gattctcaag gaaccactgg
    20521 taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag ctgatgcagg
    20581 cgcgtcacat ccagcagttt gcgcggcgta ccatccggtt tgctggcatc aaaaaccacc
    20641 cgacctttgt aacccaccac tttggcgatg gtttgcgcca gctcgcggat agtgcagtca
    20701 acgcccgtgc cgacgttaat gtgcgacagc atcggctggg tgttctccag ccagacttcg
    20761 tgcgccagct ccatgacatg aatgctcgcc gccgccatat catcgacgtg caggaattca
    20821 cgcatcggtg taccgctgcc ccataccacc acgtccggtg cattctgtgc cgtcgcctcg
    20881 tggaagcgac gcagcaatgc agggatcaca tgcgaattac tcgggtggaa gttgtcatgc
    20941 gggccataca ggttggtcgg catgactgag cggtaatcgc gtccgtactg gcggttgtat
    21001 gattcgcaca gtttgatccc ggcgattttg gcaatggcgt aaggctcgtt ggtcggctcc
    21061 agcgtgccct gcaataactc gctttctgcc atcggctgtt ttgccagttt cgggtagata
    21121 caggacgatc caagaaacag cagtttgttc acgtcgttct gatgcgcggc gtgaatgatg
    21181 ttgctctcaa tcatcatgtt ctggtagatg aaatccgccg gataggtgtt gttggcaaca
    21241 atgccgccca ctttcgccgc cgccagatag acctggtcaa tgcgttcgct ggcaaagaaa
    21301 tcatgcaccg cgcggctgtc cagcaggttc agctcgtcgc gggtgcgtaa taccagttcc
    21361 acatcaccgc gctgttcgag ctgccgcctg atggcagaac cgaccatccc acgatggcca
    21421 gcgataaaaa tgcgttgttt actcatgctt atgactccag cgcgatcgcc acgtcgtagc
    21481 cgtgagattt cagcagagag tgttttttcg ccgcttcgag gtcattagcc accatttcag
    21541 acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc gctttggtcg
    21601 ggtcgccgag cagcgtttca acttcagccg gacggaagta acgcggatca acggcgataa
    21661 tcacatcacc cggtttaacg cccggcgcgt catgcccggt gacggaaacc acaatgccct
    21721 tctcttcaac acccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc gctgccattt
    21781 ccacgaactg acgcacggag tactgaacgc cggtcgcgat aacgaaatct tccggctgtt
    21841 cctgttgcag catcatccac tgcattttta cgtagtcttt ggcgtggccc cagtcacgca
    21901 gggaatccat attgccgagg tacaggcacg actccagccc ctgggcgatg ttggcgattg
    21961 cgcgggtgat tttgcgggta acgaaggttt cgccgcggcg cggggattca tggttgaaga
    22021 gaattccgtt acaagcgtac atgccgtagg attcgcggta gttaacggtg atccagtagg
    22081 cgtacagttt ggcgaccgca tacggagatc gcgggtagaa cggcgtggtt tctttctgcg
    22141 gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa cgagttttct
    22201 tttccagacc gaggaagcgg atcgcctcca gcaggcgcag cgtacccatc gcgtcgacgt
    22261 cagcggtata ttctggtgac tcaaaagaga ccgcaacgtg gctcattgcg cccaggttgt
    22321 acacttcatc cggctgtact tcacgcaaaa tgcgcgtcag gttagaggta tcactcaggt
    22381 cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa atgtgatcca
    22441 cgcgctcggt gttgaatgac gatgcgcgac gcttaatacc atgcacctcg taaccttttt
    22501 ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg atgagagcga
    22561 cttttgacat gtcttattcc tctgtatttt tgaatttatt cagtttcaac gcgttcgcgt
    22621 atcaccactg cgggattccc ccggcaaacc acatttgccg gaagcgattt aaaaacactg
    22681 cttcgtgcac ccacgacggt gccgtcgccg attgtgacgc ctggggcaac aaagacatcg
    22741 gttgccagcc agcatttctc gccaatcaca ataggcgtgg cgttaatggt gaaatgttga
    22801 cttgcatggt cgtggctacc ggtgcataaa taactttttt gcgatatcac cgaatgtgcg
    22861 ccaatggtta tttcaccgag ggtatataaa ttgacgtcat cgccgaccca cgcgtaatca
    22921 cctaaggtta atttccacgg ataggtaatt tttactgacg gacgaataac tacgtttttt
    22981 cctatttttg ctccgaataa acgtaataaa aaagcccgcc agcgatacaa tacttgtggc
    23041 gaccaggcaa atattgttgc ctgtactgcc caccataatt gcactttaat agcgttgccg
    23101 ccccggaacc ctttcggcac cgagaaaccg cttaaatctt gcattggttt tccttatatt
    23161 cagactttgt tatataaggc tttcgtcttt gaggtagtac gttggcgtaa atgccaggat
    23221 aattcagccc aaaagccagg cacatgtaat atttgtcgtt ggactttttt cgcgtcagca
    23281 cacaattcca tattattggt ggtagatacc ccacccatgg aaaattcaga caccaggcca
    23341 ttgagttttt taaatgcata accggcttta tacattttgg ctgccagcgc gtagtcggaa
    23401 gaaactttat attccaggtc ataacgccat tttttcaagc cggatacagg gaaaaatatc
    23461 gcctgatgac tggcgggcag gctgtgataa atataccagc ccggtttggc gctacgttta
    23521 attttatgcc cgtcgccaaa atccagcagc gcatcgccgg tgatcatcat gttatctttt
    23581 tgcattttta acttacggac aaaatttgcg gcatcctgat gaaaaatatc gcccgaattg
    23641 agaaacaacg cgaacttgcc ttgtgccatc gtaatgcctt tgttcatggc gtcgtagata
    23701 ccgttatctg gctcgctgac aaagcgtagg ttatagatac cattgagatt ttccagatac
    23761 tcacgggtgc cgtcattgga accgccatcg acaacaatcc attcgaagct gatatcttcc
    23821 gcctgcgcca gatgcgccag cgaggcatgt gttttgacta tcccttcgag gttacgaaac
    23881 gcgacagtga ttatgctaag caacatgttc ttattcttac ctcgtaatat ttaacgcttt
    23941 tcgcaaaata aatggacaga cgattaaaaa tgcatattcc gggctaaata tcgaaccggt
    24001 aaaaaacagc gataccggag taaaaagata taattgcacg cgaaaattgc ggttatcacc
    24061 aaaagcgttg atcatcattt ttatcacttt ccccatgtac cacagcgata aaaacactgc
    24121 gaaccaggaa aaataaataa tcagcagata caaaccattg tctatggttt ttccgacatc
    24181 cgcaccgtta aatattccga atgatgcgac gtattcataa agtgagccaa atctgactac
    24241 accgtcaata tgggtcaaag aataaccgac catcaccagc ggaccgacaa tacgataata
    24301 cgatgacgat ccttccgtac ctaaatcgcc cagacgggtg gaaatataag gaaacgcgat
    24361 taccacacca accaggaata cagccagaga aatcaatgcc aacggtaact ttttcttaat
    24421 cgcctcttta ttcagatatt gaaatgccca ctccagcaaa taaaacagga taaaagtcat
    24481 aacccctgaa aacgatccgg ataatattat ccctgcgaga atcatagcat cggttttagg
    24541 cgttttgata ccaaactgtt tgatgctgag ccaaattgag attaatgcca gagcgaaaaa
    24601 tgccggttcg aaataaagtg ctgtcgtgcg cttgccgccg aatttaatga aattcagtac
    24661 atagctgttg ctgtaaatca gatatttcga aatcgactcc ataatactgc tgccaccggt
    24721 gaggataatt tgcgccatct ccaccgccgc cagcgccacc accagcccga tcaccagata
    24781 aaagaaacgt aatatcttgc gatggttgtg tggcgaaatc gttttaaagc gaatactcca
    24841 caccatgcca ataatgatca caatatagac aaacagcatg gttgaagtga cgtatttact
    24901 ggcatccagc gactgaccaa acagatagtt aaacgccgtg agtcccgcac caatccctaa
    24961 ggcaatcatc aattttttaa cgctgatgcg ttctaaaaac agcagtaaca agacaggtaa
    25021 aaaagtgacg atggtgatgg ggaaactttc gccaagctgg gcgattttga cgttaaccag
    25081 caggtagatc agcggcagca gcaggtagct acagattctg atagaagttg acatactcct
    25141 ccagcatctg ttgtccactg taggtggcgc ggctgcgttg gctgaactca gccagcgtgg
    25201 taccaaatat cgcctgcgcg atttccggtt tgcttaactg caccagttgc agcacgtctt
    25261 cttcgtcgac ggttttaccg ccggattttt gcaacacttc ccgtgccgcg tcgctatggg
    25321 tggcaatcac cggcacgcca attgataacg cctcacacaa aatcagcggg tagttatcga
    25381 cgcgagaact gaataccagc gcatccatct gattgagcgc gctcatcaac ttgcgcttgt
    25441 cagtttcaaa gccgtgatta accacgttgc cagcggtgaa cggcgagaac ttaccaaagg
    25501 tatgcagttc gattttgtcg cccagcgcca tcatctcacg caccagttgc tggttagtct
    25561 tgccgtcgta acgcaagtca tgcgccacca ccgcgatttt cggcttgccc tgggtttcgc
    25621 gcaccggagg caagtccgcc agaatcgctt cggttgccat atcaatacca ttattgataa
    25681 tccggcaacg ccctggaccg tacaggctat tgaaagcgtc agccacatgc tggctggggg
    25741 aaataaactg acagcccagc gccagcatct cacggaataa ctggcgtttg cccgccacca
    25801 gctggtgtgc gcgatcaatc ttcaccggcg gatagttatt taaggtcggg catttctggc
    25861 agcccgtttt ccagccttcg caaccgtcgg taaaggcgca gcgtccggta acactccagt
    25921 ggtcgtgcag cgtccagacc agagtgacgt ccggtttatg gtttttcact ttttcgcaaa
    25981 agcgcaccac gctcttaaga tttagccagt agctgtgcag cacatgaaaa tgcaggacca
    26041 ccggacccgg tgtgcgagta atggtgcgat ataactcatt gaaattgcca aacagatcgc
    26101 gattaaacag acgaaacagg gcaatattcg ccatcgcggt catccgcggc gtatgtttga
    26161 tgacctgcgg atagttctga tggctgacgc tctctttgcc gcctttgccg taaccgtaga
    26221 caaaatgtga cgccagcccc tgttgcagcg cacgctggtg gagatctaac gccacacctg
    26281 ctgccccgcc ttccgccagt cgcacattaa attgcagaat attcatttaa ttaccttcac
    26341 tcgcgctttt tctcccacca ccagcgcgtt gtccgggaca gagtcgagca ccacgctgcc
    26401 cgcacccacg gtgacgttgt taccaagcgt gatatcacca agaataatga cgttggcacc
    26461 gagttcgacg ccgttgccaa tgtgtggaca tgccatgtta tcggcaccac gattgccgat
    26521 agtgacgccg tggcgaatgg tgaaatcatc ccccgctacc acgtttttat tgatcactac
    26581 ggcgtaaccg tgatggatag taaagcggcg gccaatggtc gcggcggcct ggatttcata
    26641 accgaaaaag cattcggtga taatgcgata cagcaccagc agcggggccg cccacagatt
    26701 gttgaggacg ttctttttgc gccacaccga acaaaaatga gcaacacgat aggcaagaac
    26761 catgcagcac gggcgtaaac tccagctgtt ggcacgcaga tcttccagca tctttagcgc
    26821 ccccgaatac catcagccag acgtttgccg ttacgcaccg acagcagcgt cagcaaagtg
    26881 cgccaggtca tacgtttatt gcggatctgg tagagggtaa acagctgata ttttttgctt
    26941 gcacggtcaa atttatcttt gtgctttcgg taaaagtgga agtacccgga gaattttttc
    27001 ggtgacgagg tgatctgcat ttcaccgtga ttgatatgca ggatctgcgt cgcttcttcc
    27061 actttccacg gttcgccgta ctccaccacc atccgcagga agatgtcgta atcctgtgct
    27121 gctttcagtt cggtatcgaa caaacactct ttgaaacgcc atgcccaagt aaagacctga
    27181 ttgccaatga tattgcgttt atagaacagg cggcgtgagt acggcgattt gggatacagt
    27241 ggtaagcttg ccggttggga ataaacttct ccctggcata cgtagtcgtt agcgtacaaa
    27301 aaggcgtgag tgaccagttg ctgtttatgg gcgaggaaga cgctcagacg gttgggtgtc
    27361 cattcgtcat cgtcatcgat cccggtaatg tattcccctt gcgccagcat aatcgcctgg
    27421 ttacgtaccg cgcacgcccc gctgttaatg tcgttgtgaa tgtaggtgat acgcggatcg
    27481 ttgagggcag tgacgtactg ttgcagttgt tcccaggagg tggagcaatc atccacgatg
    27541 atcatctccc agttgctgta gtcctggcgc aggaccgatt ttatcgcccg aatcgccagt
    27601 tgttggcggt tccaggtcgg catatagatt gaaatcagcg gattctcttt cataggttgt
    27661 tctcccggct tatgagctgg tttgcttgcc ggatgcggcg tgagcgcctt atccggccta
    27721 cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga cttatattcg tattcgtaat
    27781 agccataatc ctgatacgcg ctggcgcggc ggaagatgga gttcagaatc acccctttca
    27841 ccggaatacc gttttgctca aagcggctca gactggtttc cacttctttc aatgtgttga
    27901 ccgcatagcg cgccaccatt aacgtggttc cagcgtgacg cccgacaatt gctgcatcgg
    27961 tcactgccag aatcggcggc gtatcaatca acaccaggtc gtagttttta ctcgcccagc
    28021 tcaccaattc ggcaaagcgt tcgctcatca acagttcaga aggatttggc ggcacctgac
    28081 cgcgcgggat caggtcaaat ttggcaatag aggtcggttt agctgcggta gtaatatcgc
    28141 cctggccaat cagaatttcc gacaggccat tgacgttatt ggtacccaac agctcgtggg
    28201 tgtagccttt gcgcatatcg cagtcgatca acaacacccg tttattggtc tgactgatca
    28261 ccgccgccag gttggcgcag acaaaggttt taccgattga cgggctcacc cctgtcatca
    28321 tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag actggtacgc agactgcgga
    28381 tggcttcaat cgccagatcg gttggattcc ccaccgccag tagctggctc tgtttatagc
    28441 gtttaatccc tttgatggtt ttgacgctat cgcgcgcttt ctgccattcc gacagcggga
    28501 tgctggcata gacgctgata ccatgttctt ccagcacctg cgggctttcg atgccgcgat
    28561 taaacaacga gcgcagcagc acacccacga tagagagcat caggccgagg ataattgcgc
    28621 cgaggataat cagtcctttc ttcggtttca gcacgccagg ctgggtgatt gccgggtcaa
    28681 caatgcgcac atcgccgacg gtgctggctt cggtgatttt cagctcctgc tctttattca
    28741 gcagttgcat atagacctgc tgaccagact cgacatcgcg ggtcaggcgc acaatctcct
    28801 gctgggtttt cggcatcgcc gttacgcgac cgttaagttt ggctttctct tcttccagcg
    28861 cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac tttggtgtac agcttggaga
    28921 tttccgcctc tttaaaggtc agttcgttca actgcgcgtc gatgttcacc atcgaatcga
    28981 gcaccgcttt cgcttccagc ggcagatcaa cagaatcttt atcctgacgg aaggcattca
    29041 gtttgttttc ggcaacatcg aggcggctac gtacttccgg taactgttgc gcgaggaaag
    29101 cgaggctttt cgacgcttcc gccgatttgc gctcaatatt ttgttcctga tagttacggg
    29161 cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc accggtataa gtcaggctca
    29221 gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct gttttgcagt tggttgatca
    29281 tccccaacgt ggagtatttg gtgacggtaa actcactgcc cgggcgggcg tgaatggctt
    29341 caaccatcag cgtgacgcct tcttttttca gcatctggcc cgcttgccca cgggcgctaa
    29401 agccgccatc actgctcagg gtgtagtttt tattgtccag cacattaagc gtaaacacct
    29461 ggcccgccat ctctttcggg cggttaaagg tagtcacttt caccgtctcg ttctggcgtc
    29521 ccatcagtcg ctcccagccc gccccgaaaa tcgggaacgt gtttttgctc actgcaatat
    29581 cgaggtcgag atcgtccacc gttttaccaa gcaccagacg cgaacgaatc aactggatct
    29641 cggcgtccga tgcaggcggt ttgttggcta atgccgaacc aatgtcctgc actaacgaat
    29701 tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact ataaatcggc gttgcgaaga
    29761 aggtgtaaac cacggcacag agggcaaata cagcggtgat gccaatcacc caccagcgcg
    29821 cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc atcactgccc gttaccggag
    29881 cggcatgttg ttttactttt tctgtcattc ttatacctgc tctgcgttca atgcctgcgc
    29941 ccactggcgg gcagaccgtt caagtaatgt gtacaccgct gcaaacgttt cccggctttt
    30001 gcgatacgga tcggggattt cacattcgtt atcccagtga ccaaacagca tcactttgcc
    30061 gcgcatctca ggtgccatct cgcataagcg ttcgatatgg cgcttttcca tggtcaaaat
    30121 caggtcatag ttgcgacaca ggcggcggct gatttgtcgt gcacagtggc cttccagaga
    30181 cagttgatgt tctgcggcga cgctgatagc ggtaggatca gcgcccttac cgaccagcgc
    30241 gccgagtcca gcggactcca ctttcagttc cgggtgataa cgttgcagta agcgttccgc
    30301 cgtcggggaa cggcaaatat tgccgacaca gacaactaat atgttgttaa acatgacgat
    30361 taccagttat gaatgtcgct ggctgtatcc gtcatgtaac ggacaccgct aatagttggc
    30421 aacaactgat tgatcagacg gttccagcgg gaaaccgggg cggtggtgac atacaccacg
    30481 tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg acgtggcatc ggacatatcc
    30541 agctggtaga tattggcaat cttgccatta cgcccgccct cgcctttcag cggacgaatg
    30601 acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac cttcagcatt acccagggct
    30661 tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc tctgtttctt cacttcaccc
    30721 atcacaaata ctttcagatc atcattacgc ggcacgtaga gaatatcgcc gggataaagc
    30781 agtcggttct gattgaggtc gccgttttgc atcagcgcct gcaaagaaat gcgttcttca
    30841 cgaccattgt gtgttagcac tacgttgcgc cagtcagcgg tgtcggtcag gccacctgcg
    30901 gcgttgatgg cgtcgagaat agtcagtggc acgttggtga tcgcctgttg accggattta
    30961 ttcacctgac cggagatata ggccttttgt gagcggaagg cggcgatatt aacgtccacc
    31021 tgcgggtcag cgatgtacgt cgctaagcgc ccggtaatat cactgcggat ttcagcgagc
    31081 gttttcccga ctacgtgaac cttgccgata tacgggtaaa acatagtgcc gtcaggctgt
    31141 acccagttgc cggtgtcgct ggagctgcgg tactgaccgg ctggcgtggt gagttccggg
    31201 tgatcccaga cggtgacatt cagaacgtcg cccggcccga cgcgatactg gtaattcgcg
    31261 atctcgcttt ccagcgtcat attggggcgc gctacattcg ggcgtgggcg taattggtca
    31321 atcaggcgcg gggtcagcgg ataaacattc accattttgt cgagatcgaa atcagcgtcc
    31381 tgctgtttga tgacgtcttt gcccatcgtc gacatattgc tgcccggaag tactgtgcaa
    31441 ccgcttatca aggttactga caccaataat ggcatcaatt tcattttgga tttcatcatt
    31501 gtttatttat cactttggca gagtaattat cctgtgcact attaatagca atatcgccat
    31561 gcacatttac cttgcagtta attgaataaa aatttaactg gcatcagtcc taaaaaaatt
    31621 gatttcatcc gcaggctatt gacagaataa ttcagactgg tctttcaggc atccagacac
    31681 gctaccgccc ctggcttttt agccaccaat acactgattt agcttaattt ttcaaaccct
    31741 ctctgcatac aatcgttgat gagaaagggt tattgcggaa attaacttcc gaatataagg
    31801 tgacattatg gtaattgaat attggctctc caataatgca ggaggaagtg ttacagctaa
    31861 cggaatagca ggcaaggtaa caattcggca attggctatt tttaagaatt atattaagtg
    31921 tcattcaata tggtttttag gagtttcttt ag
//
LOCUS       DAZPJP010000029        31118 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPJP010000029 DAZPJP010000000
VERSION     DAZPJP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.029"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            19..714
                     /gene="araD"
                     /locus_tag="ACHU9J_001235"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="ACHU9J_001235"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1904830.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="ACHU9J_001236"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="ACHU9J_001236"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1904831.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="ACHU9J_001237"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="ACHU9J_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1904832.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="ACHU9J_001238"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="ACHU9J_001238"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1904833.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHU9J_001239"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHU9J_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1904834.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="ACHU9J_001240"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="ACHU9J_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1904835.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="ACHU9J_001241"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="ACHU9J_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1904836.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHU9J_001242"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHU9J_001242"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1904837.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHU9J_001243"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHU9J_001243"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1904838.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="ACHU9J_001244"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="ACHU9J_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1904839.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="ACHU9J_001245"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="ACHU9J_001245"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1904840.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHU9J_001246"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHU9J_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1904841.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHU9J_001247"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHU9J_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1904842.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHU9J_001248"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHU9J_001248"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1904843.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="ACHU9J_001249"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="ACHU9J_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHU9J_001250"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHU9J_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1904844.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="ACHU9J_001251"
     CDS             complement(19713..20999)
                     /locus_tag="ACHU9J_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1904845.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="ACHU9J_001252"
     CDS             complement(21050..21991)
                     /locus_tag="ACHU9J_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1904846.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="ACHU9J_001253"
     CDS             complement(22006..22776)
                     /locus_tag="ACHU9J_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1904847.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23249..24763
                     /gene="caiT"
                     /locus_tag="ACHU9J_001254"
     CDS             23249..24763
                     /gene="caiT"
                     /locus_tag="ACHU9J_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1904848.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24794..25936
                     /gene="caiA"
                     /locus_tag="ACHU9J_001255"
     CDS             24794..25936
                     /gene="caiA"
                     /locus_tag="ACHU9J_001255"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1904849.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26065..27282
                     /gene="caiB"
                     /locus_tag="ACHU9J_001256"
                     /pseudo
     CDS             26065..27282
                     /gene="caiB"
                     /locus_tag="ACHU9J_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            27356..28909
                     /gene="caiC"
                     /locus_tag="ACHU9J_001257"
     CDS             27356..28909
                     /gene="caiC"
                     /locus_tag="ACHU9J_001257"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1904850.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGDTLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29018..29803
                     /gene="caiD"
                     /locus_tag="ACHU9J_001258"
     CDS             29018..29803
                     /gene="caiD"
                     /locus_tag="ACHU9J_001258"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1904851.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29809..30399
                     /gene="caiE"
                     /locus_tag="ACHU9J_001259"
     CDS             29809..30399
                     /gene="caiE"
                     /locus_tag="ACHU9J_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1904852.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHU9J_001260"
     CDS             complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHU9J_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1904853.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         7542 a         8377 c         8151 g         7048 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc tgaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagtccatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa aacatggcgt agaaaaaaga aattttcaat attgctttat
    22981 ggatcaccaa tattgaaagt cagatgctat ttttttactc acaacacaac ataacaaact
    23041 gattaataat caaaattaac gagaaaagcg ccctctattc acccgaaaaa aaatgtgata
    23101 acaatcacag aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    23161 aggttaataa taattatatc aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    23221 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    23281 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
    23341 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    23401 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    23461 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta ccgccagttg
    23521 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    23581 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggggcgaa
    23641 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    23701 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    23761 cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
    23821 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    23881 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    23941 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    24001 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    24061 ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    24121 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    24181 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg ctatccagat
    24241 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    24301 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct
    24361 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    24421 gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    24481 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    24541 gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg
    24601 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    24661 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    24721 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    24781 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    24841 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    24901 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    24961 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    25021 gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaacac
    25081 cttcctgcgc gaaggcacac aagagcagat cgacaaaatt atggctttcc gcggcaccgg
    25141 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    25201 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    25261 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    25321 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    25381 acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgacgtgga
    25441 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    25501 gttcgaccat gaacgtttcc tggtagccct caccaactac ggtacggcga tgtgcgcttt
    25561 tgaagatgcg gcgcgctacg ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt
    25621 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    25681 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    25741 gtgcaaatac ttctgcgcca atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct
    25801 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt
    25861 agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct
    25921 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc
    25981 tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca gtcatgcgct
    26041 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
    26101 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
    26161 ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctaggc cgacaccatt
    26221 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    26281 attttcaaag atgaaggccg cgaagcgttt ctgaaataaa tggaaaccac cgatatcttc
    26341 atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    26401 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    26461 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    26521 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    26581 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    26641 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    26701 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    26761 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
    26821 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    26881 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    26941 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    27001 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    27061 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    27121 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    27181 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    27241 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    27301 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    27361 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    27421 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    27481 gttaaatcag gagattaacc gcacgacaaa cctgttttat acgctgggga ttcgcaaagg
    27541 cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    27601 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aggaaagcgc
    27661 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
    27721 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    27781 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
    27841 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    27901 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    27961 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
    28021 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    28081 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
    28141 cgccaccgtt accgaatgta ttccgatgat gatccgtacc ttgatggcgc agccgccttc
    28201 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    28261 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    28321 ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
    28381 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    28441 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    28501 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    28561 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
    28621 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    28681 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    28741 catcaaagca tttgtggtgc tgaatgaagg tgacacattg agcgaagagg aatttttccg
    28801 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    28861 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
    28921 gggaactacc ctgccaacta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
    28981 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    29041 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
    29101 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    29161 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
    29221 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
    29281 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
    29341 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    29401 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
    29461 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    29521 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    29581 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    29641 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    29701 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
    29761 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    29821 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    29881 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    29941 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    30001 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
    30061 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
    30121 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
    30181 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    30241 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    30301 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
    30361 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcggcgtaaa
    30421 cgccttatcc aactctgctg gagagcaacc gtaggccgga taagatgcgc cagcatcgca
    30481 tccggcgata gctgtcggat gcggcataaa cgccttatcc aacccggctg gagagcaacc
    30541 gtaggctgga taagatgcgc cagcattgca tccagcaacc aggctgcgtg gtatcaacga
    30601 aatgacatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    30661 gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc
    30721 ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact tttaccagtc
    30781 gctggcactg gcatccccgt ccttccagct tattagggat catcttaact gcgcagctta
    30841 tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    30901 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    30961 cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    31021 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    31081 tggatatatc gatagtatgc aacttcaccg tcgataag
//
LOCUS       DAZPJP010000030        30078 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPJP010000030 DAZPJP010000000
VERSION     DAZPJP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.030"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHU9J_001261"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHU9J_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1904854.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHU9J_001262"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHU9J_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1904855.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHU9J_001263"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHU9J_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1904856.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHU9J_001264"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHU9J_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1904857.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHU9J_001265"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHU9J_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1904858.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHU9J_001266"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHU9J_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1904859.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHU9J_001267"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHU9J_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1904860.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHU9J_001268"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHU9J_001268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1904861.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHU9J_001269"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHU9J_001269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1904862.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHU9J_001270"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHU9J_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1904863.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHU9J_001271"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHU9J_001271"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1904864.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHU9J_001272"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHU9J_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1904865.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHU9J_001273"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHU9J_001273"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1904866.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHU9J_001274"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHU9J_001274"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1904867.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHU9J_001275"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHU9J_001275"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1904868.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHU9J_001276"
     CDS             complement(18516..18992)
                     /locus_tag="ACHU9J_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR1904869.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHU9J_001277"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHU9J_001277"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1904870.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHU9J_001278"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHU9J_001278"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1904871.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHU9J_001279"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHU9J_001279"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1904872.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHU9J_001280"
     CDS             21688..22278
                     /locus_tag="ACHU9J_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1904873.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHU9J_001281"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHU9J_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1904874.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHU9J_001282"
     CDS             22750..23445
                     /locus_tag="ACHU9J_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1904875.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHU9J_001283"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHU9J_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1904876.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHU9J_001284"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHU9J_001284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1904877.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHU9J_001285"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHU9J_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1904878.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHU9J_001286"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHU9J_001286"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1904879.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHU9J_001287"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHU9J_001287"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1904880.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7031 a         7852 c         8287 g         6908 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcatttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaaga
//
LOCUS       DAZPJP010000031        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPJP010000031 DAZPJP010000000
VERSION     DAZPJP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.031"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHU9J_001288"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHU9J_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR1904881.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHU9J_001289"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHU9J_001289"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1904882.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHU9J_001290"
                     /pseudo
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHU9J_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(3644..4030)
                     /locus_tag="ACHU9J_001291"
     CDS             complement(3644..4030)
                     /locus_tag="ACHU9J_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1904883.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHU9J_001292"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHU9J_001292"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1904884.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHU9J_001293"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHU9J_001293"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1904885.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHU9J_001294"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHU9J_001294"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1904886.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHU9J_001295"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHU9J_001295"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1904887.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHU9J_001296"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHU9J_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1904888.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHU9J_001297"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHU9J_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1904889.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHU9J_001298"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHU9J_001298"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1904890.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHU9J_001299"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHU9J_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1904891.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHU9J_001300"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHU9J_001300"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1904892.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHU9J_001301"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHU9J_001301"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1904893.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHU9J_001302"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHU9J_001302"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1904894.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHU9J_001303"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHU9J_001303"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1904895.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHU9J_001304"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHU9J_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1904896.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHU9J_001305"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHU9J_001305"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1904897.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHU9J_001306"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHU9J_001306"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1904898.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHU9J_001307"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHU9J_001307"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1904899.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHU9J_001308"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHU9J_001308"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1904900.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHU9J_001309"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHU9J_001309"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1904901.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHU9J_001310"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHU9J_001310"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1904902.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHU9J_001311"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHU9J_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1904903.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHU9J_001312"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHU9J_001312"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1904904.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7120 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 taaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg agaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPJP010000032        29079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPJP010000032 DAZPJP010000000
VERSION     DAZPJP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.032"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHU9J_001313"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHU9J_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1904905.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHU9J_001314"
     tRNA            1230..1305
                     /locus_tag="ACHU9J_001314"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHU9J_001315"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHU9J_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1904906.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHU9J_001316"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHU9J_001316"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1904907.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHU9J_001317"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHU9J_001317"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1904908.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHU9J_001318"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHU9J_001318"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1904909.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHU9J_001319"
     CDS             complement(4868..5305)
                     /locus_tag="ACHU9J_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1904910.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHU9J_001320"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHU9J_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHU9J_001321"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHU9J_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR1904911.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="ACHU9J_001322"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="ACHU9J_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR1904912.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="ACHU9J_001323"
                     /pseudo
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="ACHU9J_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="ACHU9J_001324"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="ACHU9J_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR1904913.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="ACHU9J_001325"
     CDS             10773..11441
                     /locus_tag="ACHU9J_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR1904914.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="ACHU9J_001326"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="ACHU9J_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR1904915.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="ACHU9J_001327"
     CDS             11984..13816
                     /locus_tag="ACHU9J_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR1904916.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="ACHU9J_001328"
     CDS             13806..14156
                     /locus_tag="ACHU9J_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR1904917.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHU9J_001329"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHU9J_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR1904918.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="ACHU9J_001330"
     CDS             14475..14897
                     /locus_tag="ACHU9J_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR1904919.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="ACHU9J_001331"
     CDS             14900..15532
                     /locus_tag="ACHU9J_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR1904920.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="ACHU9J_001332"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="ACHU9J_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR1904921.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="ACHU9J_001333"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="ACHU9J_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR1904922.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="ACHU9J_001334"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="ACHU9J_001334"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR1904923.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="ACHU9J_001335"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="ACHU9J_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="ACHU9J_001336"
     CDS             18711..19823
                     /locus_tag="ACHU9J_001336"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR1904924.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19820..21163
                     /locus_tag="ACHU9J_001337"
     CDS             19820..21163
                     /locus_tag="ACHU9J_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1904925.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21166..21720
                     /gene="pduT"
                     /locus_tag="ACHU9J_001338"
     CDS             21166..21720
                     /gene="pduT"
                     /locus_tag="ACHU9J_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR1904926.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21720..22070
                     /gene="pduU"
                     /locus_tag="ACHU9J_001339"
     CDS             21720..22070
                     /gene="pduU"
                     /locus_tag="ACHU9J_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR1904927.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22075..22518
                     /locus_tag="ACHU9J_001340"
     CDS             22075..22518
                     /locus_tag="ACHU9J_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1904928.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22619..22948)
                     /locus_tag="ACHU9J_001341"
     CDS             complement(22619..22948)
                     /locus_tag="ACHU9J_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1904929.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23120..24178)
                     /locus_tag="ACHU9J_001342"
     CDS             complement(23120..24178)
                     /locus_tag="ACHU9J_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1904930.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHU9J_001343"
     CDS             complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHU9J_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR1904931.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHU9J_001344"
     CDS             complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHU9J_001344"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR1904932.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHU9J_001345"
     CDS             26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHU9J_001345"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1904933.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHU9J_001346"
     CDS             complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHU9J_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1904934.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28054..>29079)
                     /gene="tsuA"
                     /locus_tag="ACHU9J_001347"
     CDS             complement(28054..>29079)
                     /gene="tsuA"
                     /locus_tag="ACHU9J_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1904935.1"
                     /translation="GALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVG
                     VFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIAL
                     FTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVMKE
                     LKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGI
                     TSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGL
                     GGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKAR
                     LATAAAN"
BASE COUNT         7668 a         7629 c         6932 g         6850 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaagcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcga ggagttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc
    20701 cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct gcgccagtgt
    20761 cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg
    20821 cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg caaattatcg
    20881 actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt atcaggatgc
    20941 gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat
    21001 cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt
    21061 aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg atggcatggt
    21121 cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc gaaagcggta
    21181 ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa
    21241 agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt tctgctgatg
    21301 ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt
    21361 gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct ttctgcaatc
    21421 agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc
    21481 gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat tcttgttcgc
    21541 gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg cgatatttca
    21601 gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg tttgttggtc
    21661 tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa
    21721 tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt aagcaggtca
    21781 cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg ggtttaccag
    21841 acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct atcatcgcat
    21901 gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg
    21961 ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag gttacccgta
    22021 cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa
    22081 cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt
    22141 ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat
    22201 acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt cagtgcctgc
    22261 gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc tttttcgcca
    22321 ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc agatttagcc
    22381 aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg
    22441 ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt gctgcataca
    22501 gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg gtgatacaca
    22561 gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt
    22621 acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc
    22681 gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac
    22741 ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc ttgatgtaat
    22801 cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt
    22861 cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt
    22921 cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga gagtcagatt
    22981 cgtttaagca accgctattc gttagggcca acattactca tagctgccgc agatgacaat
    23041 gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga
    23101 tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact
    23161 caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta
    23221 ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt
    23281 tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaagctca
    23341 gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga
    23401 aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat
    23461 tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg
    23521 cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt
    23581 ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact
    23641 cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac
    23701 cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg
    23761 tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca atcaataatc
    23821 cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc
    23881 agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga cctaaaatcg
    23941 aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta ccccagaacg
    24001 agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa
    24061 acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacta
    24121 tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc
    24181 agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca gaaggcgcgc
    24241 ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg agcgaaaaaa
    24301 ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg cgttaacaca
    24361 ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca
    24421 gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc
    24481 ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc
    24541 accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac
    24601 gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg tgtcgcagcg
    24661 taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc actccttcgg
    24721 cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct ttaccgtctg
    24781 ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct gcgtaatctc
    24841 gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc aatattaaaa
    24901 ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa
    24961 agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg ccgccttccc
    25021 cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt
    25081 caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg
    25141 tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt
    25201 ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt
    25261 tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc
    25321 ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca atgagacgac
    25381 gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt
    25441 tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt tgctgggtga
    25501 taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga
    25561 tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca
    25621 gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca
    25681 tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt
    25741 catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct
    25801 ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc
    25861 gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga
    25921 cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg
    25981 cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca tgaatctctg
    26041 gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga
    26101 acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata
    26161 gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac
    26221 taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt gtggcgctaa
    26281 agctgattag cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc
    26341 taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg
    26401 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat
    26461 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac tatttacccc
    26521 agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa
    26581 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
    26641 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
    26701 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttgctggatg
    26761 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
    26821 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg
    26881 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
    26941 gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
    27001 tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt ggggcatggc
    27061 gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc aatgccgtaa
    27121 ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc gacacattgc
    27181 gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
    27241 tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg
    27301 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt ctgcgtgaaa
    27361 atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt
    27421 cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat cgtgcagcaa
    27481 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg
    27541 ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
    27601 attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg cccgagtttt
    27661 tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat aacaaagaga
    27721 aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct
    27781 ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt gatactccac
    27841 gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc cgcccactgt
    27901 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
    27961 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
    28021 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
    28081 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
    28141 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
    28201 gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
    28261 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
    28321 aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
    28381 gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa
    28441 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
    28501 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cagttcggcg
    28561 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
    28621 cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag tctcagcaat
    28681 agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga
    28741 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
    28801 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
    28861 gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt cgtaagtcag
    28921 taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg caatcagcaa
    28981 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca
    29041 aaaacgccca cgctgcatga caaatcccag cagagcacc
//
LOCUS       DAZPJP010000033        28357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPJP010000033 DAZPJP010000000
VERSION     DAZPJP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.033"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1165
                     /locus_tag="ACHU9J_001348"
     CDS             <1..1165
                     /locus_tag="ACHU9J_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1904936.1"
                     /translation="KPIWNWKISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTR
                     PTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLD
                     TNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLL
                     KRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIM
                     ANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRA
                     ISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNA
                     RDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1355..1468
                     /gene="yhiY"
                     /locus_tag="ACHU9J_001349"
     CDS             1355..1468
                     /gene="yhiY"
                     /locus_tag="ACHU9J_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1904937.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1531..2598
                     /locus_tag="ACHU9J_001350"
     CDS             1531..2598
                     /locus_tag="ACHU9J_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1904938.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2595..5330
                     /gene="rbbA"
                     /locus_tag="ACHU9J_001351"
     CDS             2595..5330
                     /gene="rbbA"
                     /locus_tag="ACHU9J_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1904939.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5330..6454
                     /locus_tag="ACHU9J_001352"
     CDS             5330..6454
                     /locus_tag="ACHU9J_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1904940.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6527..6798
                     /locus_tag="ACHU9J_001353"
                     /pseudo
     CDS             6527..6798
                     /locus_tag="ACHU9J_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6795..7154
                     /locus_tag="ACHU9J_001354"
     CDS             6795..7154
                     /locus_tag="ACHU9J_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1904941.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7274..7675)
                     /gene="nikR"
                     /locus_tag="ACHU9J_001355"
     CDS             complement(7274..7675)
                     /gene="nikR"
                     /locus_tag="ACHU9J_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1904942.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7681..8487)
                     /gene="nikE"
                     /locus_tag="ACHU9J_001356"
     CDS             complement(7681..8487)
                     /gene="nikE"
                     /locus_tag="ACHU9J_001356"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1904943.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8484..9248)
                     /gene="nikD"
                     /locus_tag="ACHU9J_001357"
     CDS             complement(8484..9248)
                     /gene="nikD"
                     /locus_tag="ACHU9J_001357"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1904944.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(9248..10081)
                     /gene="nikC"
                     /locus_tag="ACHU9J_001358"
     CDS             complement(9248..10081)
                     /gene="nikC"
                     /locus_tag="ACHU9J_001358"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1904945.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10078..11022)
                     /gene="nikB"
                     /locus_tag="ACHU9J_001359"
     CDS             complement(10078..11022)
                     /gene="nikB"
                     /locus_tag="ACHU9J_001359"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1904946.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11022..12596)
                     /gene="nikA"
                     /locus_tag="ACHU9J_001360"
     CDS             complement(11022..12596)
                     /gene="nikA"
                     /locus_tag="ACHU9J_001360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1904947.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12707..13294)
                     /gene="acpT"
                     /locus_tag="ACHU9J_001361"
     CDS             complement(12707..13294)
                     /gene="acpT"
                     /locus_tag="ACHU9J_001361"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1904948.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13349..14398)
                     /locus_tag="ACHU9J_001362"
     CDS             complement(13349..14398)
                     /locus_tag="ACHU9J_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1904949.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14530..15747
                     /locus_tag="ACHU9J_001363"
     CDS             14530..15747
                     /locus_tag="ACHU9J_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1904950.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15751..16308)
                     /gene="dcrB"
                     /locus_tag="ACHU9J_001364"
     CDS             complement(15751..16308)
                     /gene="dcrB"
                     /locus_tag="ACHU9J_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1904951.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16381..17046)
                     /locus_tag="ACHU9J_001365"
     CDS             complement(16381..17046)
                     /locus_tag="ACHU9J_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1904952.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            17267..17512
                     /gene="tusA"
                     /locus_tag="ACHU9J_001366"
     CDS             17267..17512
                     /gene="tusA"
                     /locus_tag="ACHU9J_001366"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1904953.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17614..19812)
                     /gene="zntA"
                     /locus_tag="ACHU9J_001367"
     CDS             complement(17614..19812)
                     /gene="zntA"
                     /locus_tag="ACHU9J_001367"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1904954.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19886..20512)
                     /locus_tag="ACHU9J_001368"
     CDS             complement(19886..20512)
                     /locus_tag="ACHU9J_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1904955.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20653..21012
                     /locus_tag="ACHU9J_001369"
     CDS             20653..21012
                     /locus_tag="ACHU9J_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1904956.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21015..21284)
                     /locus_tag="ACHU9J_001370"
     CDS             complement(21015..21284)
                     /locus_tag="ACHU9J_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1904957.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21274..21870)
                     /gene="rsmD"
                     /locus_tag="ACHU9J_001371"
     CDS             complement(21274..21870)
                     /gene="rsmD"
                     /locus_tag="ACHU9J_001371"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1904958.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22020..23516
                     /gene="ftsY"
                     /locus_tag="ACHU9J_001372"
     CDS             22020..23516
                     /gene="ftsY"
                     /locus_tag="ACHU9J_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1904959.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23519..24187
                     /gene="ftsE"
                     /locus_tag="ACHU9J_001373"
     CDS             23519..24187
                     /gene="ftsE"
                     /locus_tag="ACHU9J_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1904960.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            24180..25238
                     /gene="ftsX"
                     /locus_tag="ACHU9J_001374"
     CDS             24180..25238
                     /gene="ftsX"
                     /locus_tag="ACHU9J_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1904961.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25483..26337
                     /gene="rpoH"
                     /locus_tag="ACHU9J_001375"
     CDS             25483..26337
                     /gene="rpoH"
                     /locus_tag="ACHU9J_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1904962.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26608..27711
                     /gene="livJ"
                     /locus_tag="ACHU9J_001376"
     CDS             26608..27711
                     /gene="livJ"
                     /locus_tag="ACHU9J_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1904963.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27910..28293)
                     /gene="panM"
                     /locus_tag="ACHU9J_001377"
     CDS             complement(27910..28293)
                     /gene="panM"
                     /locus_tag="ACHU9J_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1904964.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6760 a         7348 c         7837 g         6412 t
ORIGIN      
        1 caaacccatc tggaactgga aaatatcgca atacaatgta tttgatatta aattcgaaac
       61 aatgataaaa ccccagttcg ctgacagaac ctatttctcc aattgttcac ctgttgatcc
      121 cacaagtacc agaccgacct attttggcga taccgatggc tcagtcggtg cagttttgta
      181 cgcgcttttt gcaacgggac atttgggaat catggctgaa ggagaaaatt tccttagcca
      241 gttgcttaat atagaagatg aagttctcaa cgtattgcta agggagaact ttaatgagca
      301 acttgacact aatgttaata taataatcag tatcctgaac agaagagaca ttgttttaga
      361 aagtcttcag ccttatttgg ttattaataa agacgcagtc acgccatgta cgtttctggg
      421 cgaccagact ggcgatcgat ttagtaatat ctgtggcgat aaatttatca tcgatttatt
      481 aaaacgcata atgagtatca atgaaaacgt tcatgtatta gctggaaatc atgaaacgaa
      541 ttgcaatggt aactatatgc agaacttcac gcgcatgaaa ccacttgatg aggataccta
      601 ctctgggata aaagattatc ctgtatgttt ctatgacccc aaatacaaaa taatggcaaa
      661 tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac cgataacagt
      721 ctctagtgat gaaatgacca atgctctcga tccggtcgaa ttagcggcaa ttatcaataa
      781 aaagcaccat gctgtaatta atggcaaaaa gtttaaaaat agccgggcta tctcttgtcg
      841 atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg aagcactatt
      901 agcatgctca caaatgttag gtataaatca agttgttgca cataacggta atggtggaag
      961 agaatgtata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca gtaaacatgc
     1021 tggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcagggc ctgagataac
     1081 cactccctgg aagtcttacc agcatgaaaa aaacagaaat ggacttatgc ctttgatcag
     1141 aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg ttactatctg
     1201 gttgagactg ttgataaaac gtaaaaagga atgctctgtt cctgcaactg aacaggcgtg
     1261 aaacgctatc caacaggatg gataccgttt tgtcagccag ttttatagat cacacttatg
     1321 agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac tacccgtttt
     1381 cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt gtcgtttcct
     1441 tcttatcccg gatggaagaa tccgctgaac gtcgttcggc ggcagttgta acacgcgtcg
     1501 tttgcttgca gggattatgg cgggtcgatt atggataaga gtaagcgcca tctggcgtgg
     1561 tgggttgtcg ggttactggt ggtggctgcc gtcgtggcgt ggtggctgtt gcgcccggcg
     1621 ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga agtggatatc
     1681 gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca gtttgttcgc
     1741 gaaggcgaag tgctggcgaa gatggatact cgcgtgttgc aggagcagcg actggaagcc
     1801 atcgcgcaaa tcaaagaagc acaaagtgcc gttgctgccg cgcaggcttt gctggagcaa
     1861 cggcaaagcg aaactcgtgc cgcgcagtcg ctggttaatc aacgccagtc cgaactggac
     1921 tccgtggcta aacgtcatac gcgttcccgc tcgctcgccc aacgaggggc tatttctgcg
     1981 caacagctgg atgacgatcg cgccgccgct gagagcgccc gagctgcgct ggaatcggca
     2041 aaagctcagg tatcggcttc taaagcggct atagaagcgg cccgtaccaa tatcattcag
     2101 gcccaaactc gcgtcgaagc cgcgcaagcc actgaacggc gtattgccgc agatatcgat
     2161 gacagtgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc tgagccaggg
     2221 gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga cgtctatatg
     2281 actttcttcc tgccaaccga acaggcgggc acgctgaaac ttggcggtga agcccggctg
     2341 atcctcgatg ccgcgccaga tctgcgtatt ccggcgacca tcagttttgt cgccagtgtc
     2401 gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact gatgttccgc
     2461 gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt caaaaccggt
     2521 ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga cgacctcgtg
     2581 gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc gcgcaactgg
     2641 cgggcgtgag ccagcattat ggaaaaaacg ttgcgctgaa caatattact ctcgatattc
     2701 cggcccgctg tatggtcggg ctgattggtc cggatggcgt cgggaagtcg agcttattgt
     2761 cgttgatttc cggtgcccgc gtcattgagc agggcaatgt gatggtgctg ggcggcgata
     2821 tgcgcgaccc gaggcatcgc cgcgacgtct gcccgcgcat cgcctggatg ccgcaggggc
     2881 tgggcaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt ttcgctcgcc
     2941 tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa tgagctgctg accagcacgg
     3001 ggttagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg aagcaaaaac
     3061 ttgggctgtg ctgcgcgtta atccacgacc cggaactgtt gatccttgat gagccaacaa
     3121 cgggggttga cccgctctcc cgctcccagt tctgggatct gatcgacagt attcgccagc
     3181 ggcagagcaa tatgagcgtg ctggtcgcca ccgcctatat ggaagaggcc gaacgcttcg
     3241 actggctggt agcgatgaat gccggagaag tgctggcaac tggcagcgcc gaagagctac
     3301 ggcagcaaac gcaaagcgct acgctggaag aagcatttat aaatctgtta ccgcaagcgc
     3361 aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac gcagagattg
     3421 ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt gatcacgtta
     3481 atttccgcat tccacgcggg gagatttttg gttttcttgg ttcgaacggc tgcggtaaat
     3541 ccaccaccat gaaaatgctc accggactgc tgcccgccag cgaaggtgag gcgtggctgt
     3601 tcgggcaacc ggttgatcca aaagatatcg atacccgccg tcgggtgggc tatatgtcgc
     3661 aggcgttttc gctctataac gaactcaccg tgcggcaaaa ccttgagtta catgcccgtt
     3721 tgtttcacat cccggaagcg gaaattcccg caagagtggc tgaaatgagc gagcgtttta
     3781 agctcaacga cgttgaagat gttctgccgg agtcattgcc gctcggcatt cgccagcggc
     3841 tttcgctggc ggtggcggtg attcatcgcc cggagatgtt aatcctcgat gagcctactt
     3901 ctggtgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat ctctcgcgcc
     3961 aggacaaagt gactatcttc atctctaccc actttatgaa cgaagcggaa cgttgcgacc
     4021 gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg tacaccgcag gaactggttg
     4081 agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag gaagcggcag
     4141 ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga caccacccac gcgccgcgtc
     4201 agggatttag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg gaactgcgac
     4261 gcgatccagt acgttcgacg ctggcgctga tgggaacggt gatcctgatg ctgataatgg
     4321 gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac cgcgaccaga
     4381 ccgtcagtag ccaggcgtgg acactcaacc tctccggttc ccgttacttt atcgaacagc
     4441 cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg
     4501 cgattgagat cccgcccaat ttcgggcgcg atatcgcgcg tggtacgcct gtggaactcg
     4561 gcgtctggat cgacggagcg atgcccagtc gtgccgaaac ggtaaaaggt tacgtgcagg
     4621 caatgcacca gagttggctt caggatgtgg cgagccgaca atcgacaccc gccagccaaa
     4681 gcgggctgat gaacattgag acgcgctatc gctataaccc ggacgtaaaa agcctgccag
     4741 cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg ctaagcgccc
     4801 ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg acccccacca
     4861 cccgtagcga atttttgctt ggcaaacagc tgccgtacat cgcgctgggg atgctgaact
     4921 ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa ggcagtttcc
     4981 tgacgctcac cctggcggcg ctgctgtata tcatcattgc caccggaatg gggctgctga
     5041 tctccacctt tatgaaaagc cagattgccg ccattttcgg aacggcgatt atcacgttga
     5101 tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa gggcctggac
     5161 gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc ggaacgttct
     5221 cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg atagccatcc
     5281 cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga tgcgccattt
     5341 acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg ataaagcgat
     5401 gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga ccgttacgcc
     5461 aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc agttatcgaa
     5521 ccggatcgtt aacagcttct atcgtccgtg gtttttgcca ccggagatga tcaccgccga
     5581 tgagatggat gccggactgg acgccggacg ctataccttc gcgataaata ttccgcctaa
     5641 ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg tcgatgccac
     5701 gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca acggtgaagt
     5761 gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc tggaaacccg
     5821 gatgcgcttt aacccgaacc tcgatcccgc gtggtttggc ggggtgatgg cgatcatcaa
     5881 caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg agcgtgaaca
     5941 cggcacggtg gaacacttac tggtgatgcc gataacgccg tttgagatca tgatggcgaa
     6001 gatctggtcg atggggctgg tggtgctggt ggtatcggga ttatcgctgg tgctgatggc
     6061 gaaaggtgta ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc
     6121 gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag cgcgttcaat
     6181 gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc tttccggtgg
     6241 atccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga ccatgccgac
     6301 gacacacttt gttagcctcg cgcaggccat tctctaccgg ggtgccggat tcgaaatcgt
     6361 ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca ttgcgctgct
     6421 gcgattcagg aagacgattg ggacaatggc gtaaggaagg gaaacttgac ttcatactgt
     6481 gatgtcatac tattgatgtc ataaagtgaa gtcataagga catgccatga gaaccaaggt
     6541 acaggcttta cggaagaaaa aaaatacatt ggatcaaata tttaaaactc ctgttcctca
     6601 agggatcaag tggtctgata tagagtcact ggttaaagca ttaggcggag aaattaagga
     6661 aggaagaggt tcgcgttgta agttcatact aaatatgagc gttgcgtgtt tccatcggcc
     6721 tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc gtgcgtgact ggttataagt
     6781 ataggggtaa aaccatgatc aaactgaaga cgccaaactc tatggagatt gccgggcaac
     6841 cagctgttat tacctacgtg ccagaactga atgcatttcg tggcaaattc ctgggcttgt
     6901 caggttattg tgattttgtc tctgacagca ttcaggggct gcaaaaagaa ggggaacttt
     6961 ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag
     7021 aaaagattaa gacctttacg ttacgctatc cagaatcgct gagtgaacgc cttaataatg
     7081 cggctgcgca gcaacaagtc tctgttaata cctatattat tgaaacactg aatgagcgtt
     7141 tgaatcattt ataaatttta gcggcgtaca ttacgcagaa tatctgcctt tacaggatct
     7201 atctatgcaa ctgaacttaa ttttataaat taagttcagt taatatcttt tctttcctcg
     7261 tttcagaaat aactcaatct tccttcggca agcactgcaa atgcccatgc cgcacgccgc
     7321 gctgggcgat aacgtcatcg gcaaaatgct gcacgtcgcc catatcacct ttcagcacgg
     7381 cgatttccag acagtcgtcg tgattgatat gcacatgcag cgtggcgacg gagagatcgt
     7441 ggtgatggtg ctgggtggag acaatgcggc tggctaagtc gcgtttttcg tgttcataca
     7501 cataggagag caccgcgaaa ccttgcgtgc cgtgctgctg agtggcctcc tgggccaggg
     7561 cgctacgcag aatatcgcgg atagcttcgg aacggttgtt ataaccacga cgctggctca
     7621 ggctgtccag cgtctccagt aaatcgtcat caagcgtgat ggtgactcgt tgcatttgcg
     7681 ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg ggtaataccg cgttttgtag
     7741 cacacgtccg gcgtcagagg aaaaggttaa tttatctccc accacctggg tttcgacgat
     7801 ttgtccgttg tccatcacca tcacccgctg acagaagcgt tccaccaggc gtaagtcgtg
     7861 ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc tttttcagca ggcgaatgac
     7921 gcccgcctgt aacacgagat caaggttaga aacggcttca tccagaatca gtagtttcgg
     7981 ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc tggccgccgc ttaactgcgg
     8041 tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc ttcagcattt cgctggcgcg
     8101 cgccagttgt tcggattttt tcagtgatag caggtggcgc atcggttcac gcaggatctc
     8161 gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc tgaaatacca tctgaatatc
     8221 gcggcggaac gctttacgct gggcgcgatt gagtttcgcc agcggttcgc cacgccagct
     8281 aatattcccc tgcgcgggcg attctaaacc caccagcaac cgcgcgaggg tacttttccc
     8341 gcagccgctg cgccccagca gggcgacggt ttcgccgctt ttcagggtca gggaaacgtt
     8401 attcagcacc gcctgatgtt gatgttttcc gttaaatcca ccgtgggcat agtgatggga
     8461 aaggccggag acgttaagta aagtcatgat gccagctcca taccgtagag ggcgagatga
     8521 gcggaaacca ggctgcgcgt taccgcatgt ttgggggcgt taaacagcgt ttctacatcg
     8581 ccctgttcga caattttacc ttgtgacatc accgccacgt catccgccag acgcgccaca
     8641 acgcccatat catgggtgac cagcagcatt cccggcgctt gtttttgcat aatgctttcc
     8701 agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg
     8761 atgataaacg gtgattcaca cagcaccgcc atcgcaatca tcatgcgctg caacatgccg
     8821 ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg cgttttccag ccccaccgct
     8881 tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc ctaacgccag gcaggtttcc
     8941 cgcgcgtggg tgtgcatggt gtgtagcgga ttaaaggcgc tgcgcgggtt ctgcatgatg
     9001 gtggcaattt tgatgccgcg cagggcgcag ggataaaccg ctttgccatc ggctaaaatt
     9061 tcccccgccg tctggcgaac gccagcgggc aaaatgccca gcgtcgcggc gcaggttaat
     9121 gatttcccgc tgccgctacc gccgactaac gccagcacgc gcccgcgttg cagggttaac
     9181 gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgcga tattacgtag ttcaatctgt
     9241 tgcggcatta gtgtgcgtgc tccgtcacca gatgaggatc cagatgatcg cgcagtgcgt
     9301 cacccaccag gttaaaggcc atcacgctga taaacagcgc cagccccggc cagaacattt
     9361 gcagcggctg ggtccagata tactggcgcg cgtcgttaat catcacgccc cattcggcgg
     9421 tcggcgcggt cacaccgagg ccaaggaaag acatccccgc gacgtgcagc atcatatggc
     9481 cgatatccag cgttgccagc accagcagcg aagggatcac cgcgcctgcc agatgatcga
     9541 caaacacccg cacatggccc gcaccggaaa gccgtgacgc cagcacaaac tcgcgttggc
     9601 gtagtgaaat caccaagctg cgcaccatgc gtgcatacca cgcccagtgc gacagggcga
     9661 tggcgataat tacgttggtc agcccggtgc cgagcacgcc aaccataaag aacgacagaa
     9721 tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat ggtggcctga tcaacgcgcc
     9781 cgccaatcaa cccggcgctg ccgccaataa ccagccctaa tgtcagcacc agcagcaggc
     9841 aggccattac cgaaccgagc gacacgcggg tcgctgccat cagccgcgag aaaatatcgc
     9901 gacctaagtg atcggtgccc agccagtgct gcgcatccgg cgaaagcagg cgcgacggca
     9961 aatcaatcgc ctgggggtca tacggcagcc accactggct ggtgagcgca atcagcgcca
    10021 gcagggcgat aatgatcagc gccaggcgta ccgaccagcg ggaagagagg aaaaagttca
    10081 cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc gcggcgttga gcaaatcgac
    10141 aatcaaatta cagaccacaa aaaccaccac catcatcagc gtaaagcact gaatcaccgg
    10201 atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg acgcccggcc aggcaaagat
    10261 gttttcgata atcatcgtcc cgccaatcag ttcgccgatg tgcatcccca cggcggtgat
    10321 catcggcagc gaggcattgc gcagaatatg gcggcgttcg gtctgtttgt cgttcaggcc
    10381 gcgcagacgc gcccaagtga cgtgacgctg accggcgacg tccagcatac tggcgcgcag
    10441 cagtcgcgcg ttaatcgcca gcgacataaa ggcaatggaa accgcaggca aaatgatgtg
    10501 ctgccagccg ccgtaaccca tcgcgggtag ccattgcaga tacaccgaaa acgccatcac
    10561 cagtaaaaac gccagccaga agttaggcat cgacacgcca agaaacgcga tgaaacgtac
    10621 ggcgaaatcc ggcagacggt cgcgatggcg cgccgcccag ataccgagcg gtacggaagt
    10681 gagcagaatt aataccagcg ccgcacctgc aagttccagc gtggcgggca ggaagttcag
    10741 catatcgtcc agcaccgggc gttggctggc gaatgagata ccaaagtcaa gatgcagcgc
    10801 cttccacaac caggtaccgt actggacgta cagcggctga tccagcccga gcatggtgcg
    10861 ggtagaggcc agcatctccg gcgtcggcgg caggttagat aaacgcagat aatcgagcgc
    10921 cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc accgaggcgg cgagcaccat
    10981 cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac attaaggttt caccggttta
    11041 atctgttcga acggaatttc ggtggcgatc ggcgcgtagg ggatattacc cagctccggt
    11101 tttgatacca ccatcatcga gatgtagctg ataggcagat aaaccgcctc gtcatgcaga
    11161 cgggtcaaaa tgtcgcgata cagcgcctga cgttgcgttt cgtcatgggt cgccagcact
    11221 tcgccgatct ctttatcaat cagcggcttg tcggctaatc cttgctgtgc ctggaagtca
    11281 gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag
    11341 gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac gagcatagat actgctctct
    11401 tcttcgccaa tcagcgagac atctgcgcca atctggcgca tatcagcctg aatgatttcc
    11461 gccatcgatt tgcttaacgc atcggtgccg atgaacgaca gttcaatgcg cagcggctga
    11521 ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc aaccggcttt ttccagcaac
    11581 tctttcgctt tctgcggatc gtactggcgc ggtttcaggc cgagattagc gtaaggcaca
    11641 gaaggggcaa acagggtgtc ggcgacctgc tgggtgccat ataacgcgtt atcaatcagc
    11701 gattttttgt ttaccgcgta attaagagct tcacgtactg ccagctcgtt ggtgggggct
    11761 ttggcggtat tgagcgccag catcacggtt tcgatcggct gtgacagttg ggtgtggtaa
    11821 gccggattct ggctaaagcg ggcgaaggta tcgagcggta ataacccttc gtttccgtac
    11881 agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg tagtcgggtc cgggatgacg
    11941 ttaaaggtga tctttttaat cgctggcttt tcgccccagt agttttcgtt acggacgaag
    12001 acatcgtact gattcagttt cgattcctgc aaaatccacg gtccggtgcc aatcggcgct
    12061 ttaattccgt tcatggtttc atggttttta aactgcgagg gagcgataaa acggaaagga
    12121 cggggcaggg ccagttcttg caggaaagga tagtaggcgc ttttcagggt aatttgcagc
    12181 tctgttttac tgagtgcttt aacatcaaca atctggtttg ccagctccag ccaggcgtga
    12241 cgttgacggt tatcgagcac tgcgcggaag ttttctgccg ccgcctcggc atcgaacggt
    12301 tcaccgttgg agaatttcac gtcatcacgc agggtgaagg tccaggtttt accatcttct
    12361 gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
    12421 aatggttcat aaaccatgct ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt
    12481 agtggcccga cattcaccgg ccaggcggta gtgatttcat ctggtgcagc ggcatggacg
    12541 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtgga gagcatggta
    12601 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata
    12661 ctgattacct gttctggatc aatagatggg cagtcgggtg ggcggatcag ttaactgaat
    12721 cgatccattg cacactgtcg gcggtgagcg taaagggggt gggggtgcag atcgcaaggc
    12781 ttaaattttc tagctgacaa tggctgactg acagcgagga gtgataggtg ctatctacgc
    12841 tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga
    12901 tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatttca gcgtgttccc
    12961 cgaggctgaa cacggcgttc gccagccagc gccagttggc gcgcgggcga atcacttcga
    13021 tatcgcagcc gacttcgcct tcatcgctca acagcagagc gatatcgtca ccgctatggc
    13081 ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt tcgccataga
    13141 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt
    13201 cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagtgga gctgcgctta
    13261 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
    13321 gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg
    13381 gccccagtaa aatcgccagt ttgctaccgc ctttggtggt ttccatccag attttacaca
    13441 cactggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta
    13501 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggtt
    13561 ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc agaatacatt
    13621 cgtaaacacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcgc
    13681 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgca
    13741 cccccatcag ctccagcccc agccagacga tgacacctgt ccataaactg agtagcgtct
    13801 tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg taatcctgcg atgtgaatct
    13861 gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa
    13921 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
    13981 gcgctgtggt gaaggtaacc actttttccg agtccatccg ctgcagcatt cgctccggcg
    14041 acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct
    14101 ccttattaaa cttcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga
    14161 ccagcgcggt tagtgcgatc agcatcacca ccactacaat cgtaatggca acggggcgtt
    14221 gtactccccg acgaataaac caggtaacga gcgggttgag gacgatggca aaaaacagcg
    14281 ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
    14341 gcgaggccag cttgagcaga atgtgcatgc ccgttttatc gggttgaggg atttccattg
    14401 gggcttcctt gtgacttttt gtattaagtg tagcgggagt cacgccagca atattctgat
    14461 tcttactgct gattttcacg tcagcacgtg gtaaaaatga aacactgttg taaaaatgtg
    14521 gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg cctgaatttg
    14581 cgcattgtct ccattgtcat gtttaacttc gccagctacc tcaccatcgg gttgccgctc
    14641 gctgtattac cgggctatgt ccatgatgtg atgggcttta gcgctttctg ggcaggattg
    14701 gttatcagcc tgcaatattt cgccaccttg ctgagccgtc ctcatgccgg acgttacgcc
    14761 gatttgctgg gccccaaaaa gattgtcgtc ttcggtttat gcggctgctt tttgagcggt
    14821 ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactt
    14881 tgcctgggac gcgtgatcct tgggattggg caaagttttg ccggaacggg atcgaccctg
    14941 tggggcgttg gcgtggttgg ctcgctgcat atcgggcgag tgatttcgtg gaacggcatt
    15001 gtcaattacg gggcgatggc gatgggtgcg ccgttaggcg tcgtgtttta tcactggggc
    15061 ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat tttgttggcg
    15121 atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg cgcggtgctt
    15181 gggcgcgtct ggctgtacgg tatggcactg gcactggctt ctgccggatt tggcgtcatc
    15241 gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg
    15301 acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt
    15361 atcggcggct taaacgtggc gatgatttgc tttagcgttg agataatcgg cctgctactg
    15421 gttggcgtgg cgactatgcc gtggatggcg aaagtcggcg tcttactggc gggggcaggg
    15481 ttttcgctgg tgttcccggc attgggcgta gtggcggtaa aagcggttcc gcagcaaaat
    15541 cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgaccgga
    15601 ccactggctg ggctggtgat gagctgggcg ggcgtaccgg tgatttatct ggcggcggcg
    15661 ggactggtcg caatcgcgtt attactgacg tggcgattaa aaaaacggcc tccggaacac
    15721 gtccctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt
    15781 ttctgcggtg gtctgcgctt tttgctgatc gtcagctggc agagtaattt gcatggtcag
    15841 cagttgatta cccacgttac ccagaataac ggaagagtac gccgtctggc ctttcgcgga
    15901 gataatactg tctaactgct gcattttgtg acctttcagc tcaatggctt tgttggttac
    15961 cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc agacgcttcg ccaacaccgc
    16021 cagatcttct ttcggatcat cgcccatgat gacgatgact gctttctgcc cggtggcgtc
    16081 ggaccagaca tgcatgttat tggcctgcgt tcccagctta ccgctctggt cggtcatatc
    16141 cgctggcagc gagaaactta acttgccatc aagcaggttg acgggattcc cggtagcgtt
    16201 actttccgcg accgaaccct gcgccgtagc gttagtgtct ttatcatcac aggccgcaag
    16261 ccccataacc agcaggccaa ttccgacata tttaaccaga ttgcgcattg acttcttcct
    16321 ttcgataaac ggccataacg gctcattcat ccatcttatc acaactctga taacgaacct
    16381 ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc agtcttttca acagaatatt
    16441 gagtaatacg ccatacattg gcaggaagaa aacgatactg attaacactt tgaaacagta
    16501 atcgaccagc gcgatttcca tccagtgttc agccataaag gcatccgggc tacgccagaa
    16561 ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg
    16621 tgccagccac cagcggcgac tctgacgcag gcggttaaaa acgtgcacgt cgaggatctg
    16681 tcccagcgcg taggccatga aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg
    16741 ggcgagtgcg ccgaatccct gccaggaacc catatagaat agcgacgaga tgacgtagga
    16801 gatcagcaac gccgggatca tcaccgcaaa gatgatacga cgggccagcg gtgcgccaaa
    16861 aatacgcacg gtcaggtcgg tagcaaggaa aataaacggg aagctaaacg cgccccaggt
    16921 ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga tagttactgg aggtgatcac
    16981 cagcagatga aataacgata accagaacaa cgccttatag cgttgagttt gcgagaaaac
    17041 gttcatattg tacctttttg attaaccatt ggggtgaggg aacccaatac gtacgacacg
    17101 tctgttacct taacataata acgtgcttac ccttttttcg agccgccgca tgatactgct
    17161 ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt aacctggttt gcttacggac
    17221 tcacagggcg taaactagcg ccgttttttt atgtgatgag aagaaaatga ccgatctctt
    17281 ttccagccct gaccacacac tcgacgcact tggcctgcgc tgcccggaac ccgtaatgat
    17341 ggtgcgcaaa actgtgcgca atatgcagcc tggcgaaacg ttgctgatta tcgccgacga
    17401 tccggccact acccgcgata ttcctgggtt ttgtaccttt atggaacacg aactggttgc
    17461 gaaagagacg gatggactgc cttatcgtta tttgattcgt aaaggcggtt gatggggaaa
    17521 gattggcttc gatgccgcct tttcccctca ccctaaccct ctccccagag gggcgagggg
    17581 accgatcgcg ctcaatgttg cgatcggttt gccttatctc ctgcgcaaca atcttaacgc
    17641 attcgctgtc accagcaccg tcgcacccgt atctgccagc accgccagcc acaggccggt
    17701 catccctaac agcgtggtga cgaggaagat ccctttcagc cccagcgcaa tggtgatgtt
    17761 ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc atttgcacca ggccgcgcag
    17821 gtggttatgg gttaatgctg catcagcggt ttccagcgcc acgtctgtgc cactgcccat
    17881 tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg ttaataccat caccgaccat
    17941 cgccaggggc gcatgttgat tcagcttggt caccgctttg actttatctt ccggcaatag
    18001 gcccgcttta aactccagcc ccagctcccc ggcgattgcc gccgctgcac gtggattatc
    18061 gccggtgagg atcactcctt tgacccccag cgcgttcagt tcactgatgg cagttgcagc
    18121 atcggcgcgc agggtatcct gcaatgcaat gatacccagc acgtcatcat tacgtactac
    18181 cagcacaacc gtttgcccgg cgctttccag ttcattaatc aaaccagcaa atgcatcagc
    18241 gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg ttaacctgcg cttcaatgcc
    18301 agacccgacc agcgcccgct gtgattcggc ggtgggaatg gcgagttcag caacctgtgc
    18361 ttcgcgtacg atggcttgcg ccagtggatg cgtcgcgcct tgctcgaccg ccgccgccag
    18421 tgtcagcagt tcagattcac taatacccgt tgccggatga atcgcggtaa cgcgcggttt
    18481 accgacggtc agcgtaccgg ttttatcaaa cgccacctga gtaacacgac ctagttgttc
    18541 cagcgccgct ccgcctttaa tcaacgcccc acgacgcgct gccgccgcca gcccggaggt
    18601 aatcgccgca ggcgttgaga taactaacgc acacgggcag ccaatcagca gcagcgtcag
    18661 ccctttataa atccactcct gccagctggc ggcaaacagc agcggtggca ccagcgtcac
    18721 cagcagagcg acggccataa tcgcgggcgt atagatacgg ctgaaacggt cgataaaccg
    18781 ctcaatgggg gcgcgacgct cttcggcttc ttcgatcagt ttcagaatcc ggtcaatggc
    18841 gctggcaccc ggttctgaca gcacttccag cgttaccaga cggtctacgc tggtggctcc
    18901 tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg gattctccgg tcagggcgct
    18961 ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg gcaggcaaac gcccacctgc
    19021 ggcgacttca atcacatcgc caggccgcag gctgttaatc gccacctctt cccgctcacc
    19081 gttacgcagg cgcgtggcgg tttctggttt cagcgccatt aacgcgctaa cgccctgacg
    19141 cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc aaaaacagca gcaacaccat
    19201 cgcagcttca gccgttgcgc caataaacag tgcaccaata gcggctacgc tcattaaggt
    19261 ttcaatggcg aagtagctgc cggatttgat cagccgtaat gcctgacgag caatcgggta
    19321 cagcccaacc agcgtggtcg cgataaacgc cagttgcccg aacggatgat tgaactgctc
    19381 cagaccccag ctgattgcca tcatcacgat tagtgtaatc agcggcagat tctctttcag
    19441 gcgcgatgct tgtggctcgt cggcggcctg ctcatcgcgc agggaatagc ccgctttttg
    19501 caccgcagat tcaacttgtg cacgaatgtc attgtcggca tcaaccacca gtttttcggt
    19561 ggcgaacaac acctgcacct gattcacacc tgcaagctgg cgcacggcat tttctacctt
    19621 gcgcgcacag gcggcgcagt ccatgccact gactttccag ctatagcggg tgccggagac
    19681 gttttcagag agcgttggcg agctggagca tgcgccgtcg cagcaacagt cgttggcgtt
    19741 ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg gctttcttgc cgtgattgtc
    19801 aggtgtcgac atggcatact ccggttaagt tttttctcat taaccgaagg atacactctg
    19861 gagtcgactc cagagtcaag ttttatcaga gatacagtga gcggatgatg aggaagtgac
    19921 cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg gaagcgtcgg cgatagtggc
    19981 tccccagcca gacaaagtta ctgataaaca gcaacgaagc gccaacaaac gcagagagcg
    20041 ccggagcggt cggacggaag aaccacagtt cacctgccag ccacaccatc accagcgtca
    20101 tgccgataaa cgtgcagata ggccaacgat actcttccag ccgcgtccag ataatcgcca
    20161 gtaacagcgc acccagaacc agcaacacca gtggcagagg ccagaagaaa gagagcgtca
    20221 tctgactggc gaaatagatg gtgtacagca ggtgcgagag gaaaaacgcg ccgatggcgt
    20281 acatcagacg ttgacgtggc aacagggtta gcgcatcgcc cagcagtgag gcgcacagcc
    20341 ctgccagcac cagatagcta atggcgtcga acatcggcgc ttgccaggcc agtaacagca
    20401 ggagaagaag ggttaacggt ttaaacaccc agcgttgcca ggttggccca cgatacgatg
    20461 catccacaga tagccatgcg gaaagacaga cagcgataaa cgaccaaagc atcttagttc
    20521 cttgaattcg ttattttttc gtaagcggaa tgcctacggt cttgcttcca gtttagtggc
    20581 gagggtggac ggatgacaac accataactg gcagggtatg cttatttccg cattttccga
    20641 tgagggataa agatgagcaa accacctctt ttctttattg ttatcattgg cttaattgtc
    20701 gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa aagctgataa tgatatggct
    20761 ccgctccagc aaaagctggt ggtggtgagc aacaagcggg aaaaaccgat taacgatcgc
    20821 cgttcgcgcc agcaggaagt gactccggca ggcaccagta tgcgctacga ggcaagcttc
    20881 aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg acgcccagca gtaccacgcc
    20941 ctgacagtgg gcgataaagg tacgctgagc tataaaggaa cgcgctttgt tagctttgtt
    21001 ggcgaacaat aaggttattt tttaacttta aatttcttct gccagaccag cagttcaaac
    21061 acgccgaaaa ggaaaatccg taccttttcg gcggtggtca tctgcgggcc atctttcggt
    21121 aaagtggatt tcaatagcgc cagctgcata ccatgcatca gcacggtaaa aatcagcgcc
    21181 acgttaacga aaatatttag cgggcgcggg aagggatgca ccagattgag gattaaaaat
    21241 ccccaaacgc agagcattaa caaacgacca atattaatca gcatcacttt ctccttgtgc
    21301 ttcgcgttga tacagccgat aagccacctg acccgccact ttttcccgat gtaacgacca
    21361 gtttgccgga acggtgggca gaccgttttc gacttcgctt tcgacataaa tcagggcttc
    21421 gtcagccagc cagccgttat cttccagtaa atttatcgtc tcttctaaca agccacggcg
    21481 gaacggtgga tcgacaaaca cgatattatg cggtgtgcct ttttgcgcca ggaatgacat
    21541 cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt gtcgccagat tcttaattaa
    21601 ctgctgagaa actgcacgat ccatctcaat taacgtggcc cccgcagcgt agcgcgataa
    21661 cgcttccagc cccagcgcgc cgctcccggc gaagcaatcc agacattggg cgtcaacaat
    21721 gaccggagcc agccagttaa acaacgtttc gcgtacgcgg tcggtggtgg ggcgcagacc
    21781 tgggctatca ggaaccggga gtttacggcc tcgccactgc ccgccaataa tgcggatttg
    21841 gccgctgccg gaatgattcg gttttttcat gataaattgc tcaatccgcc agataacaca
    21901 tacttgcagg cggtgatgtg aattaagtta agtgatagac tatttcatca tttttttagc
    21961 tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca gcgaggagtg tagtcgcaaa
    22021 tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg ctttggtcaa aaagagcaga
    22081 ccccggaaaa agagacagaa gttcagaatg aacaaccggt tgtagaagaa atcgttcagg
    22141 cgcaagagcc tgtgaaggct tcggaacaag ccgttgaaga gcagccgcag gcgcatactg
    22201 aagccgaggc ggaaactttt gctgccgacg ttgtggaagt cactgaacag gttgttgaaa
    22261 gtgaaaaagc gcagcctgaa gcggaagttg ttgcacagcc ggaaccggtc gtagaagaaa
    22321 cgccggagcc agtggctatc gaacgtgaag agctgccgtt gccggaagac gtcaacgccg
    22381 aagaggtttc gccagaagag tggcaggctg aagcggaaac cgtagagatt gtcaaagcgg
    22441 cggaagaaga agcggctaaa gaagaaatca ccgacgaaga gctggaagca caggcgctgg
    22501 cagccgaagc agcagaagag gcggtgatgg tggttcctcc ggcagaagaa gagcagccgg
    22561 tggaagaaat cgctcaggag caggaaaaac caaccaaaga aggtttcttc gcgcgcctga
    22621 aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc ctgttccgcg
    22681 gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga gcagcttttg atcgccgatg
    22741 tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca tcccgcaagc
    22801 agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggt gagattctgg
    22861 cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc ctgatggtgg
    22921 gcgtcaacgg tgtgggtaaa accacgacga ttggtaagct ggcacgtcag tttgagcagc
    22981 agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg gttgaacagc
    23041 ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc ggggcggatt
    23101 ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc gacgtcctga
    23161 ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag ttgaagaaaa
    23221 tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg ctgactattg
    23281 atgccagcac cgggcagaac gcggtaagcc aggccaaact gttccacgaa gctgttggct
    23341 taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta attttctcgg
    23401 tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt attgaggatt
    23461 tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag gattaacaat
    23521 gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc tgcagggcgt
    23581 tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg gcgcagggaa
    23641 aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga aaatctggtt
    23701 tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc gccgccagat
    23761 tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg ataacgtggc
    23821 gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg tgtcggcggc
    23881 actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc tttccggcgg
    23941 tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg tactgctggc
    24001 ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac gtctgtttga
    24061 agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca acctgatttc
    24121 acggcgttcc catcgcatgc tcaccctgag cgatggtcac ttgcatggag gcgtgggcca
    24181 tgaataagcg cgatgcaatc aatcatattc ggcagtttgg tgggcgtctg gatcgcttcc
    24241 gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc accgaaacgc gcgaaatcct
    24301 cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc tatgccttcc
    24361 acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg gtgatggtta
    24421 tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac gttaatcagg
    24481 cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa acgctggacg
    24541 atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg gagaaagtga
    24601 actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt tttggtggtg
    24661 cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc ccgaaactcg
    24721 attttcaggg gacggaatca ctcaatacgc tgcgtgatcg tatcacgcag attaacggca
    24781 ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg accgggctgg
    24841 tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc ctcgtcatcg
    24901 gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg cagaaactga
    24961 ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca ctgctgggat
    25021 tttctggcgc attgttgtca ttaattttgt cagaaattct ggtgctgcga ttgtcatcgg
    25081 cggttgcgga agtggcacag gttttcggaa cgaagtttga tatcaatggt ttatcgttcg
    25141 atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca gcgtggcttg
    25201 ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt atactctttc
    25261 cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg tatttcatct
    25321 ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt ggataaaatc
    25381 actgtctgat aaaacagtgg atgataacct cgttgctctt aagctctggc acagttgttg
    25441 ctaccactga agcgccagaa gatatcgatt gagaggattt gaatgactga caaaatgcaa
    25501 agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc taacgcgtgg
    25561 ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta ccatggcgat
    25621 ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca tattgctcgt
    25681 aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa catcggcctg
    25741 atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc cttcgccgtt
    25801 cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat cgtcaaagtt
    25861 gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaaccaa gcagcgtctg
    25921 ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt aaccagcaaa
    25981 gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga cctgtcttcc
    26041 gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga taaatcatct
    26101 aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa ccgtctgacc
    26161 gacgcgatgc aaggtctgga cgaacgcagc caggacatca tccgcgcgcg ctggctggac
    26221 gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc cgctgaacgt
    26281 gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga agcgtaattt
    26341 ccgctattaa gcagagaacc ctggatgcga gtccggggtt tttgtttttt gggcctctac
    26401 aataatcaat ttcccctccg gcaaaacgcc aatccccacg cagattgtta ataaactgtc
    26461 aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt agcagagtat gctgctaaag
    26521 cacgggtagc catgcataaa acgaaataaa gtgctgaaca acaacatcac aacacacgta
    26581 ataaccagaa gaatggggat tctcaggatg aacataaagg gtaaagcgtt actggcagga
    26641 tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt cgcggtcgtg
    26701 ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccaag agtttaccgg cgcagagcag
    26761 gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca aatcgtaaaa
    26821 tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt cgttaacgac
    26881 ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc gtctgacatc
    26941 tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga gctgaccgcc
    27001 cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg gccgacggcg
    27061 gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca cgacaaacag
    27121 caatacggcg aaggtctggc acgagcggtg caggacggcc tgaagaaagg caatgctaac
    27181 gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctctacgct ggtggcgcgt
    27241 ctgaagaaag agaatatcga cttcgtttac tacggcggtt accacccgga aatggggcaa
    27301 atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc ggaaggtgtg
    27361 gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag
    27421 ccgaagaact acgatcaggt tccggcaaac aaacccattg ttgacgcgat caaagcgaaa
    27481 aaacaggacc caagcggcgc attcgtttgg accacctacg ccgcgctgca atctttgcag
    27541 gcgggcctga atcagtctga cgatccggct gaaatcgcca aatacctgaa agcgaactcc
    27601 gtggataccg taatgggacc gctgacgtgg gatgagaaag gcgatctgaa aggctttgag
    27661 ttcggcgtat ttgactggca cgccaacggc acggcgacag atgcgaagta atcattaatc
    27721 ggcaactttg ggttgccgcc aaattgctaa tatcgagtac gttgcttcat gccggatgcg
    27781 gcgtaaacgc cttatccggc ctataagatc caaagaaatc agtaaattgc aacacacatt
    27841 gtaggcctga taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca
    27901 acttgacgat taacgcttct cccagccgcc ctgttgcgcc gtaaatccca gtgcctgcat
    27961 aaacgctgtc atcacaccgc gatcttccac gcctgcatcc gctatccacc agcatgaaac
    28021 gccaggattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacgtcgggt
    28081 gacttcccgc acacgcaggg aatccagtgc tccctcggtg ccgcttaagg ttacccgtac
    28141 ggcagcgagc aggcgctcgt taaaacgcgc ggcgtagata cggtggttat cgtcaacctg
    28201 taaagaggaa ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct
    28261 aaagttttct aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg
    28321 gaaccagtgt agcgaaataa tttaatcgga ggctttc
//
LOCUS       DAZPJP010000034        28109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPJP010000034 DAZPJP010000000
VERSION     DAZPJP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.034"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHU9J_001378"
     CDS             complement(<1..215)
                     /locus_tag="ACHU9J_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1904965.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHU9J_001379"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHU9J_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1904966.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHU9J_001380"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHU9J_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1904967.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHU9J_001381"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHU9J_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1904968.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHU9J_001382"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHU9J_001382"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1904969.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHU9J_001383"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHU9J_001383"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1904970.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHU9J_001384"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHU9J_001384"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1904971.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHU9J_001385"
     CDS             complement(4604..5326)
                     /locus_tag="ACHU9J_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1904972.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHU9J_001386"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHU9J_001386"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1904973.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHU9J_001387"
     CDS             6526..6744
                     /locus_tag="ACHU9J_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1904974.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHU9J_001388"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHU9J_001388"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1904975.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHU9J_001389"
     CDS             complement(7478..7678)
                     /locus_tag="ACHU9J_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1904976.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHU9J_001390"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHU9J_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1904977.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHU9J_001391"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHU9J_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1904978.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHU9J_001392"
     CDS             10175..12088
                     /locus_tag="ACHU9J_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1904979.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHU9J_001393"
     CDS             12088..13110
                     /locus_tag="ACHU9J_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1904980.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHU9J_001394"
     CDS             13104..13322
                     /locus_tag="ACHU9J_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1904981.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHU9J_001395"
     CDS             13376..14245
                     /locus_tag="ACHU9J_001395"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1904982.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHU9J_001396"
     CDS             complement(14300..14704)
                     /locus_tag="ACHU9J_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1904983.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHU9J_001397"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHU9J_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1904984.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHU9J_001398"
     CDS             15689..17779
                     /locus_tag="ACHU9J_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1904985.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHU9J_001399"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHU9J_001399"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1904986.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHU9J_001400"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHU9J_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1904987.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHU9J_001401"
     CDS             complement(19747..20349)
                     /locus_tag="ACHU9J_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1904988.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHU9J_001402"
     CDS             complement(20339..20506)
                     /locus_tag="ACHU9J_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1904989.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHU9J_001403"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHU9J_001403"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1904990.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHU9J_001404"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHU9J_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1904991.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHU9J_001405"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHU9J_001405"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1904992.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHU9J_001406"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHU9J_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1904993.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHU9J_001407"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHU9J_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1904994.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHU9J_001408"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHU9J_001408"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1904995.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6788 a         7560 c         7114 g         6647 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgccaagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gccgctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattc
//
LOCUS       DAZPJP010000035        28050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPJP010000035 DAZPJP010000000
VERSION     DAZPJP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.035"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHU9J_001409"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHU9J_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR1904996.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHU9J_001410"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHU9J_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHU9J_001411"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHU9J_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHU9J_001412"
     CDS             complement(1702..3318)
                     /locus_tag="ACHU9J_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1904997.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHU9J_001413"
     CDS             3614..4330
                     /locus_tag="ACHU9J_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1904998.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHU9J_001414"
     CDS             complement(4327..5988)
                     /locus_tag="ACHU9J_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1904999.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHU9J_001415"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHU9J_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1905000.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHU9J_001416"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHU9J_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1905001.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHU9J_001417"
     CDS             7443..7775
                     /locus_tag="ACHU9J_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1905002.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHU9J_001418"
     CDS             complement(7777..8991)
                     /locus_tag="ACHU9J_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1905003.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHU9J_001419"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHU9J_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1905004.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHU9J_001420"
     CDS             complement(10153..11490)
                     /locus_tag="ACHU9J_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905005.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHU9J_001421"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHU9J_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHU9J_001422"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHU9J_001422"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1905006.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHU9J_001423"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHU9J_001423"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1905007.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHU9J_001424"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHU9J_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1905008.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHU9J_001425"
     CDS             15153..15809
                     /locus_tag="ACHU9J_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905009.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHU9J_001426"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHU9J_001426"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1905010.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHU9J_001427"
     CDS             complement(16782..17180)
                     /locus_tag="ACHU9J_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1905011.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHU9J_001428"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHU9J_001428"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1905012.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHU9J_001429"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHU9J_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1905013.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHU9J_001430"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHU9J_001430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1905014.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHU9J_001431"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHU9J_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1905015.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHU9J_001432"
     CDS             23741..23821
                     /locus_tag="ACHU9J_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905016.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHU9J_001433"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHU9J_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1905017.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHU9J_001434"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHU9J_001434"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1905018.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHU9J_001435"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHU9J_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1905019.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHU9J_001436"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHU9J_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1905020.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHU9J_001437"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHU9J_001437"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1905021.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7571 c         7158 g         6561 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtgtc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcactcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZPJP010000036        27943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPJP010000036 DAZPJP010000000
VERSION     DAZPJP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.036"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            203..865
                     /gene="acrS"
                     /locus_tag="ACHU9J_001438"
     CDS             203..865
                     /gene="acrS"
                     /locus_tag="ACHU9J_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1905022.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(868..1047)
                     /locus_tag="ACHU9J_001439"
     CDS             complement(868..1047)
                     /locus_tag="ACHU9J_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1905023.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1131..2015)
                     /gene="yhdJ"
                     /locus_tag="ACHU9J_001440"
     CDS             complement(1131..2015)
                     /gene="yhdJ"
                     /locus_tag="ACHU9J_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1905024.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(2101..2397)
                     /gene="fis"
                     /locus_tag="ACHU9J_001441"
     CDS             complement(2101..2397)
                     /gene="fis"
                     /locus_tag="ACHU9J_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1905025.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2423..3388)
                     /gene="dusB"
                     /locus_tag="ACHU9J_001442"
     CDS             complement(2423..3388)
                     /gene="dusB"
                     /locus_tag="ACHU9J_001442"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1905026.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3717..4598)
                     /gene="prmA"
                     /locus_tag="ACHU9J_001443"
     CDS             complement(3717..4598)
                     /gene="prmA"
                     /locus_tag="ACHU9J_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1905027.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4610..6061)
                     /gene="panF"
                     /locus_tag="ACHU9J_001444"
     CDS             complement(4610..6061)
                     /gene="panF"
                     /locus_tag="ACHU9J_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1905028.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(6051..6293)
                     /locus_tag="ACHU9J_001445"
     CDS             complement(6051..6293)
                     /locus_tag="ACHU9J_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1905029.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6402..7751)
                     /gene="accC"
                     /locus_tag="ACHU9J_001446"
     CDS             complement(6402..7751)
                     /gene="accC"
                     /locus_tag="ACHU9J_001446"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1905030.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7762..8232)
                     /gene="accB"
                     /locus_tag="ACHU9J_001447"
     CDS             complement(7762..8232)
                     /gene="accB"
                     /locus_tag="ACHU9J_001447"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1905031.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9210..10184)
                     /locus_tag="ACHU9J_001448"
     CDS             complement(9210..10184)
                     /locus_tag="ACHU9J_001448"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1905032.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10336..12276
                     /gene="csrD"
                     /locus_tag="ACHU9J_001449"
     CDS             10336..12276
                     /gene="csrD"
                     /locus_tag="ACHU9J_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1905033.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12581..13624
                     /gene="mreB"
                     /locus_tag="ACHU9J_001450"
     CDS             12581..13624
                     /gene="mreB"
                     /locus_tag="ACHU9J_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1905034.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13690..14793
                     /gene="mreC"
                     /locus_tag="ACHU9J_001451"
     CDS             13690..14793
                     /gene="mreC"
                     /locus_tag="ACHU9J_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1905035.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14793..15281
                     /gene="mreD"
                     /locus_tag="ACHU9J_001452"
     CDS             14793..15281
                     /gene="mreD"
                     /locus_tag="ACHU9J_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1905036.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15290..15883
                     /gene="yhdE"
                     /locus_tag="ACHU9J_001453"
     CDS             15290..15883
                     /gene="yhdE"
                     /locus_tag="ACHU9J_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1905037.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15873..17342
                     /gene="rng"
                     /locus_tag="ACHU9J_001454"
     CDS             15873..17342
                     /gene="rng"
                     /locus_tag="ACHU9J_001454"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1905038.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17410..21210
                     /gene="yhdP"
                     /locus_tag="ACHU9J_001455"
     CDS             17410..21210
                     /gene="yhdP"
                     /locus_tag="ACHU9J_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1905039.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21366..22811
                     /gene="tldD"
                     /locus_tag="ACHU9J_001456"
     CDS             21366..22811
                     /gene="tldD"
                     /locus_tag="ACHU9J_001456"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1905040.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22939..23868)
                     /gene="aaeR"
                     /locus_tag="ACHU9J_001457"
     CDS             complement(22939..23868)
                     /gene="aaeR"
                     /locus_tag="ACHU9J_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1905041.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24051..24254
                     /gene="aaeX"
                     /locus_tag="ACHU9J_001458"
     CDS             24051..24254
                     /gene="aaeX"
                     /locus_tag="ACHU9J_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1905042.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24262..25194
                     /gene="aaeA"
                     /locus_tag="ACHU9J_001459"
     CDS             24262..25194
                     /gene="aaeA"
                     /locus_tag="ACHU9J_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1905043.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25200..27167
                     /gene="aaeB"
                     /locus_tag="ACHU9J_001460"
     CDS             25200..27167
                     /gene="aaeB"
                     /locus_tag="ACHU9J_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1905044.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27259..27531
                     /locus_tag="ACHU9J_001461"
     CDS             27259..27531
                     /locus_tag="ACHU9J_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1905045.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27587..27850)
                     /gene="yhcN"
                     /locus_tag="ACHU9J_001462"
     CDS             complement(27587..27850)
                     /gene="yhcN"
                     /locus_tag="ACHU9J_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1905046.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6730 a         6981 c         7404 g         6828 t
ORIGIN      
        1 aaagaggcag aattaaaacc tcgtaaattg aaatatatat tcatgtggcg aatgtatttt
       61 aggtaaataa tatatattat ttatttgacg atttatagca actcaatgtt accctctcgt
      121 ataaatacac attagatgat agattaacct tcgctatttt ctcaccctgt gtcgaatata
      181 tttatttcct gaataattaa tcatggcaaa aagaaccaaa gccgaagctc tgaagacccg
      241 gcaagaactg attgaaactg ccatcgccca gtttgcgcaa catggcgtaa gcaagacgac
      301 gttcaacgac attgccgatg ccgctaacgt tacgcgtggc gctatctact ggcacttcga
      361 gaacaagacc caactgttta atgagatgtg gttgcaacag ccttcattgc gggagttaat
      421 ccaggaacac ttgacggctg gattagagca tgacccgttt caacaattgc gtgaaaaatt
      481 gattgtcggc ttgcaatata ttgccaaaat tccccgccag caggcgttgc tgaaaatctt
      541 atatcacaaa tgtgaattta atgatgggat gctggccgag ggagtgatac gcgaaaagat
      601 gggctttaat ccgcagactc tccgcgaagt attgcaggcg tgtcagcaac aaggttgtgt
      661 agcaaataac ctcgatttag atgttgtgat gattattatt gatggtgcct tcagcggaat
      721 tgttcaaaac tggttaatga atatggcggg ttatgatctt tataaacaag cacccgcgct
      781 ggtcgataac gtattaagaa tgttcatgcc agatgaaaac ataacgaaat taattcatca
      841 aacgaatgaa ttaagtgtca tgtaaattca cattctgtcc tgaaaattca gttcgtcaca
      901 gttaaccagt tttagtggat aaactgagtt catattacgg catttgtctg tttctgttgt
      961 cagcagttca atccactgca tcagtaaagt atcaaacaga aaaacgaaga cagcgaaaac
     1021 gacgagccac caatacttgc gaatcattac ctgagcctgt gcatgtttaa ccaagtctgg
     1081 caggaaatat acacgaaatg aataatctga aaaccaggct tacacttcct ttactttgca
     1141 atgagatcgg ggtcaacttc gcttaaccgg cttcgttttg acaggttgcc ggtctttctt
     1201 tttttcactt tcgccagttc ttccgcggag taatgcgacg cgacatccag ccgtcgaagc
     1261 cccattttga tgtactcgct gttgatctca ataccaatga attttcgtcc gctggcgatg
     1321 gctacggcac cggtagtaaa gctaccggca aacgggtcga gaacgatatc gcctgggttg
     1381 gaagaggcga gaataatgcg tttcagtaag gcttccggtt tttgcgtcgg gtggttctca
     1441 tattcatcca tcaaataacg cacgcgcgga aaatcccaga cgttacccgg tactttttga
     1501 tgattgtatg gctgtggagg atttttgcga taatcgatca acgcgcgctg cgatccggtt
     1561 ttggcttcta ccagaatagc atcaccgttg aatgtgtagt tctttgcgtc tttcaccatc
     1621 atcaggatgg gttcgtacat ggagccgtag tgttttttcg cctgtactcc agaactgtca
     1681 tatgaccaga cgatgcgact tttgatggta aaaagcttgc ggcactggag atcgataaag
     1741 ggcatgtttt ccgtactgtt cataatgtac atgctgccct gctttttcag aacgcggtgg
     1801 cactctacaa tcacttcaaa cagccagtcg ataaacagat cttctttcca ggcttcgatc
     1861 agaccatcaa aatttttacc gatgttatac ggtgggtcgg caaagatcag atcgacgctt
     1921 tcagtaggta gctttttaag ttcggcaagg gcatcaccgt gaataatggt cttagcttca
     1981 ttaccaaacc gggtcggttc acatcctgtt ctcatggtca ctccctttgt gacacctata
     2041 aaaaaggcgc ttccccatgc cgagtagcgc ctttttaatc aagcatttag ctaacctgaa
     2101 ttagttcatg ccgtattttt tcaatttttt acgcagcgta ccacggttga tgcccatcat
     2161 cagcgcagca cgggtctggt taccacgggt gtattgcatc accatgtcca acaggggctg
     2221 ttctacttca gccagtacca gctcatagag gtcattcaca tcctgaccat tcagttgagc
     2281 aaaatagttc ttcagtgcct gtttaaccga gtcacgcagg ggtttttggg ttacctgatc
     2341 ctgagagtta acggtagaaa cggtcagtac gtcagaattt acgcgttgtt cgaacatagt
     2401 tctgtcagct ctttatttct gtttacgcaa aattttcgaa gtatgcctcc aacgcctcca
     2461 gctgttcgct ggcatcctca atggcgttga atgtgcgccg aaactggtca tttggagcgt
     2521 gttcctggag ataccaggaa acgtgtttac gtgcaattcg gtaccctttt gccggaccat
     2581 aaaagtcatg cagttcccga acgtgcgcgc aaagcaagcg cttaacctct gccaaaggca
     2641 gcgggggcag caactcccca gtgtccagat aatgctggat ttcccgaaag atccagggtc
     2701 ttccctgagc tgcgcggcct atcatcaggg catccgcccc tgtatagtcg agcacagctc
     2761 tggctttaag cgggtcagta atgtcgccat tcgcgataac cggaatggaa actttctgct
     2821 taactgcccg aatactgtcg tactcagctt ctccattgaa caaacaggcg cgtgtacggc
     2881 catgaatggt cagagcctga atgccacagt cttcagccag ttgggcaatc tcttcgcagt
     2941 tacggtgttc cggtgcccag ccggtgcgaa tcttcagggt aacaggaacg tccactgcat
     3001 tgacgacctc ggtaaggatc gatttaacga catccgggta ctgcaagagg gctgaacctg
     3061 cgagcttgcg attcactttt ttagccgggc aacccatatt gatatcaata atctgggcac
     3121 cgctttccac gttaatacgt gctgcatctg ccatttcttt cggatcgcta ccagcaattt
     3181 gcacggtgcg aataccgggt tcatcaatgt gcaccatccg taaacgagat ttgtcgcttt
     3241 cccaaacctg tgggttagaa gacatcatct cggatactgt caatccggct cccatctcgt
     3301 agcacaacgt ccgaaaaggt ctgtctgtaa tgccagccat gggcgctgcg atcaggcgat
     3361 ttctgagctg atattgtccg atgcgcatga gttaagaaat gaccatactg tgactgcaag
     3421 gcggcgtata ttacgcattt tttgcacgag atgaaaggcc aaactttgac caatcctctg
     3481 agatggatca aagaattgca tttaaaatga gcgtggtgag ataattactc ataaaaatca
     3541 tcatattaga aaatagtgac taaaatttac actcaaagaa atttgagtaa gttctcaatt
     3601 tttctttatg aatgaaaatt ttggcacgca aatttgcgta aataaccggc aaatttacgt
     3661 gccttttgtg agcttgctcg cacttcgccc cgcgtcaccc tacggcgatg cgaaggttaa
     3721 ttcttacgac cggtaatgcg gcaccactct tctttttcca cgaccgggtc cagtgcgaag
     3781 ctgtcgacat aagcttcaca aacgctctct gcctggcttg ccagaatacc ggaaaggccc
     3841 agcaaaccgc ctgaaaccgg caggacgctg attaacggtg ccagttcacg taatgggcct
     3901 gcaaggatgt tagcgaccac cacgtcggct ttcatttctt ctggctgatc tttcggtaag
     3961 tagagttcca gacggtcaga aacgccatta cgttcggcgt tgtcgcggct agcctgaatc
     4021 gcctgcggat cgatatcaat accaatggct tttgctgcac ccagtttcag cgccgcgata
     4081 gccagaatgc cggaaccaca gccaaagtcg atgactattt taccggttaa atcgaggctg
     4141 tcgagccact gcaggcacag agaggtagtt ggatgggtac ccgtaccaaa cgccagccct
     4201 ggatctaaca tcacgttgac ggcgttttcg tccggcacat cacgccagct agggcagatc
     4261 cacagtcgtt caccaaagcg catcgggtgg aaattatcca tccattcgcg ctcccagtct
     4321 ttatcttcca gttgttcgat tttatgcgcg aagcctgcgc cgagcagcgg atggttttcc
     4381 agaatcgcca ccacgtcgtt catatcggtt tcagcgtcga acagaccaat cacatcggtg
     4441 tcgccccaca ggcgcgtttc gcccggcagc ggctcaaata ctggcgtatc gtgggtatcc
     4501 tgaaaagtga tagaaacggc acccgcttcc atcagcgcat cgctaagatc ttcagcgttc
     4561 gcgccggtgg tgttcagttt cagttggatc caaggcatgg caaaactctt tatttatcag
     4621 tagtcaaaac ggtagcttgc gggacggatg taccgaaacg gtttccgacc aggaaagcca
     4681 gcaaacttag tagtaacgag ggcacgatag ggtggaagcc caggtactga atattcagcg
     4741 tcgcgagtac ggcatacagc acgccgccaa cgatcatcgc acttagcgcg cctttggcgt
     4801 tggcgcgttc ccagtaaaga cccagcacca gcggccacag gaaaacggct tccagcccac
     4861 cgaaggccag caaattcagc cagatgatca tttctggcgg cttccaggcg gcaagcagca
     4921 gcaacgcgcc gagaactaac gtaattaccg ccgacatccg cttcagacgc gtctcgtttt
     4981 gcatttgatc cggacggata ttcagataga gatctttaat gatcgtagcg gaactttgta
     5041 gcaattgggc gttaattgtc gacatgatcg cagccatagg tgcagccaga aagatcccgg
     5101 cagcaaacgg tggcagcact tttaccatta acgtcgggat caccagatcc ggtacggtga
     5161 gatcggggat cactgcacga cctaacgctc cggctaggtg cataccgaac atcaggattg
     5221 cgactacaat cgtaccgatg atgatccccc ggtgtaccgc tttgctgtct ttataagaga
     5281 tacagcgcac cgcagtatgc ggcaggccaa tcacgccaaa gcacaccagt acccagaacg
     5341 acgtcataaa ggcaggcgac agaatatcgt cagcgccttg tggcgtaacc agttgcggat
     5401 cgatggtttg caaggtctgt actgcgttgc ttaagccgcc agcggcatgt accacgccaa
     5461 taagcagcac aacggtgcca atcagcatca caagcccttg catggtgtcg ttcagcacgc
     5521 tggcgcgaaa gccgccaaag gcggtataca acgcaatgct gataccgaaa atcagcagcc
     5581 cggtttcata aggaataccc gccgcggttt ccagcaggcg cgcaccgccg ataaactgca
     5641 cggtcattgc cccaacgaac gcaaccagca aactcaaact cgccagccac accagaagac
     5701 gactctggta gcgggcaaac agcatgtcgt tcagcgtcac tgcattgtag cggcgcgcaa
     5761 gaatcgcaaa cttcttgccg agaataccga gtgaaagcca gactgcagga agctgaatca
     5821 tcgccagcaa tacccagccc agcccgtatt tataagcagc tcctggcccg ccgataaacg
     5881 aactggcact gatataggtc gcggtgagcg tcatcgccag cacaataccg cccatagagc
     5941 ggctgccgag gaaatactca ttaaggaagg tgccggtgct ccgtttacgc atcgcataaa
     6001 ccgagatacc gaacaccacc accagatagg cgaccagcgg tagaattact tcaagctgca
     6061 tcgtcatcct ccagtgggat atcgcgatag ataaatttca ccatcgccca gcacagtcca
     6121 ataaacagta gcggcgtcag gatgcaggcc atctcaaacc agcgcggaaa gccggtaaaa
     6181 ccgggggcaa cgccaggtaa gtaagcggct actaaccaaa ctgccagata caaaagggtc
     6241 agccccagcg cccagcgcgc ctctttatgg gcctgaacaa aacgagtgtc cattttttgt
     6301 ccctgatggg tgaagaaagc gaggattgta ccttatgggg gttgtcgatc cccagtaata
     6361 aaaaaggccg gaaaatccgg ccttttgacg cttttgcagt cttatttttc ctgaagaccg
     6421 agttttttct ccagatagtg gatgttagtg ccaccatgct ggaagttctc gtcattcatg
     6481 atgcggatct gcagatcaac gttggttttg ataccgtcga tgatcagctc ctgcagcgca
     6541 ttcttcatgc gggcaatcgc cacgtcacgg ttttcaccgt agcaaatcag cttaccgatc
     6601 attgagtcat agtacggcgg tacggtgtag cccgcgtaga tatgagactc ccaacgtacg
     6661 ccaaaaccgc caggtgcgtg gaaacgggtg attttgcctg ggcttggcag gaaggtgttc
     6721 ggatcttcgg cgttgatacg acattccacc gcatggccgc gaacgtgaac ttcttcttgc
     6781 ttgatcgaca gcggttgacc ggcagcgata cggagctgtt ctttgatcag gtcaacgcca
     6841 gtgatcattt cggtaaccgg gtgttctacc tgaatacggg tgttcatttc gatgaaatag
     6901 aactcgccgt tttcgaacag aaactcgaaa gtacctgcac cgcgatagcc gatatcaaca
     6961 cacgctttag cgcaacgttc gccgatgtag cgacgcagtt ccggggtaat gcccggtgct
     7021 ggcgcttctt cgaccacttt ctggtggcgg cgctgcatgg agcagtcacg ttccgccaga
     7081 tagatagcgt tgccctgacc gtcagccagt acctgaatct cgacgtggcg aggattttcc
     7141 aggtatttct ccatgtaaac catatcgttg ctgaaagcag ctttcgcttc cgcacgggtc
     7201 atggagatgg attgtgccag ttcagcgtcg ccgcgcacta cgcgcatacc gcgaccaccg
     7261 ccgccgccgg aggctttgat aatcaccgga taaccaatgc gtttagcaat ggcacggttt
     7321 ttatccatat cgtcgcccaa cgggccgtca gaacccggta cgcaagggac gcccgctttt
     7381 ttcatcgccg cgattgcgga tactttgtcg cccatcaggc gaatggtttc tgctttcggg
     7441 ccgatgaaga taaagccgga gcgttcaacc tgctcggcaa agttggcgtt ctcggagagg
     7501 aagccgtaac ccggatggat tgctactgcg ccggtgattt cagcggcgct gatgattgcc
     7561 gggatgttca gataactttt tactgacgga gcagggccaa tacagaccgt ttcatctgcc
     7621 agtaatacgt gttttagatc gcgatccgcg ctggagtgca cagcgacagt cttgatgccc
     7681 agttctttac aggcacgaag aatacgcaat gcaatctcgc cgcggttggc aataacaatt
     7741 ttatccagca tgttcgcctc gttactcgat gacgaccagc ggctcgtcaa attctaccgg
     7801 ttgtccactt tcgaccagaa ttgctttcac ggtaccggat ttgtccgctt cgatctggtt
     7861 catcattttc atggcttcaa cgatgcacag ggtatcgccc acgttgactt tctgacccac
     7921 ttcgatgaac gcttttgcgt ccgggcttgg ggtgcggtag aaagtaccaa ccatcgggga
     7981 acgtacgatg tgaccactga tttccgctgc tgctggcgct tccatggaag gaacggtcgc
     8041 cggagcggct gcgttagatt gagctggctg ctgcatcatt ggtgcagcgt aagcttgttg
     8101 catcacaggg aaacttgcgg caggagctgc acggctaatg cgtactgact cttcgccttc
     8161 agaaatttcc agttcggaga tgcctgattc ttcaaccagc tcgatcagtt ttttaatctt
     8221 acgaatatcc atgagtgggt tccgtactct ttgtttagtg tgattgtgac aggcgtttca
     8281 ccgccgtctg taaagcgtat gccccgacag ctgtatgcat agcgataaat tccagcaggc
     8341 cgggacgccg gtctattttg ccccagagta tcaatgttag acttgacgga cattgtgcag
     8401 cgatctgctg ctatcctccg gcaaaaaaca aaatatacct tcgttgcgcc aatgtcacct
     8461 tttccgcagc gcaaaaactg cgtcagggag gacgaggtcg cacattataa cgatttcgta
     8521 gcaattggca gctaaatact ggtctcatca gggaagataa tcaacagcta acatgtaaat
     8581 aaccttcaac accgtgtaat ttgcaacaag ccgcacaatt cacgaaatta gcgccatcat
     8641 cgacggaact tcttataacg taaggtgaaa agctgtaaag ccagccctgc gaagatgagg
     8701 ggctgcaatg agataatctt cacagaccac aaataatgta gggatgccag gatcgcgaca
     8761 agatagacga cattacgcag cggttgctgg ggattaccca attttcactg catcgtctgg
     8821 atagatgtga atgctaaagc acgcaaaatc aaccgactga caattctcag cattaaatag
     8881 gttcgagtgg ttaatttgta gtcaaataaa atcagattat tcaccccatg gtaccagaaa
     8941 tcagtaacag gttaagcgta gtgtcgccca ggcaaagcac cataaaccta acaggcgacg
     9001 gaggcgtatc aataatggct gtttagagga gtatcaatgc gcatcaattt gtggtcagtc
     9061 gaaatcatga ctttccagtt tggatgatgt tgtacaactt cctgaccaag gtgttcgacg
     9121 aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga ataatgatgt caaaatgagt
     9181 tccctctctt attattcctt gctaaatggt tagttaacct tcaccagcgt gcgaccctgg
     9241 atctggttat taatgatggc ctcggcaaag ttcggtgcct ctgacagaga tatctctttt
     9301 gccgcctggg tatagaatga ttccggtaaa tcggcgacca gtcgctgcca ggcttgtgcg
     9361 cggcgttctg gtggcgtcat tactgaatcc accccttgca aacggacatt acgcagaata
     9421 aatggcatga ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc cgccacgcag
     9481 ccgccgtaat tcatttgcgc cagcactttt gccagcactt tgtcgccaac agtgtcaatt
     9541 gccccagccc agacttgttt ttccagagga cgggattcgg caaactcatc acgagggaga
     9601 atacggctgg cacctaaact tttcagatat tcatgggtac tttcgcgacc ggaaacggca
     9661 acgacctgat aacccaactt atgcagcagc gccacggcgg tactgccgac gccaccactg
     9721 gcacccgtca cgacaatctc cccgtcctgc gggcgaacac cggcatcttc cagcgccatc
     9781 acacacagca tggcggtaaa accggcagta ccgataatca ttgctttacg cgcgtccagc
     9841 ccttgcggca tggcaaccag ccagtcacct ttcactcgcg cctgctccgc cagcccaccc
     9901 cagtggtttt caccaacgcc ccagccagtg agtaacacct cctgaccggc atgaaaacgc
     9961 ggatcttcgc tggtgcgtac agttccggca aaatcgatcc caggaatcat cggaaaatta
    10021 cggatgattt ttcccttacc ggtaatcgcc agcgcatctt tatagttcag gctcgaccag
    10081 tgaacatcga ccgtgacatc gccctccggc aggcgacttt cgtccagagt ctgtactgat
    10141 gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg tcctcatgtg
    10201 catgatggat tggaaaataa tttctgaaga ttatactcgc taatggaaaa gcaaagccga
    10261 tgaagcgcaa taaattgcga gataaatctg atttgctagt atgcccgctt cctcactatc
    10321 ggagttaaca caaggatgag attaacgacg aaattttcgg cctttgttac gctgctcacc
    10381 gggttaacaa tttttgtgac tttgctgggc tgttcgctaa gtttctacaa cgcaattcag
    10441 tataagttta gtcatcgcgt tcaggcggtg gcgacggcga ttgataccca ccttgtgtcg
    10501 aatgacttca gcacattaag gccacaaatt accgaattaa tgatgtcggc agatatcgtt
    10561 cgtgtagacc tgctccatgg tgataaacag gtttataccc tggccagaaa tggtagttat
    10621 cgtccggttg gctccagcga tctgtttcgc gaactgagtg ttccgttgat aaagcatccg
    10681 gggatgtcgc tgcgtctggt ttatcaggat ccgatgggca actatttcca ttctttaatg
    10741 actaccgcgc cgctcacggg ggcgattggc tttatcattg ttatgctctt cctggcggta
    10801 cgctggttac aacggcaact tgccgggcaa gaattgctgg aaacccgggc tactcgtatc
    10861 ttaaacggtg agcgtggctc taatgtgttg ggaaccatct atgaatggcc gcccagaacc
    10921 agcagtgcgc tagatacgct gcttcgtgaa attcagaacg cacgcgaaca acacagccgt
    10981 cttgatacgc tgatccgctc ttatgccgcc caggacgtga aaaccggcct caataaccga
    11041 ctctttttcg ataatcagtt agcaacgtta ctggaagatc aggagaaagt aggtacccac
    11101 gggatcgtga tgatgattcg tctgccggat ttcaatatgt tgagcgatac ttgggggcac
    11161 agccaggttg aagaacagtt cttcactctg actaatctgc tgtcgacatt tatgatgcgc
    11221 taccctggcg cactgctggc gcgttaccac cgcagtgatt ttgctgcgct gttaccgcac
    11281 cggacgttaa aagaggcaga gagcatcgcc ggtcagttaa tcaaagccgt cgataccttg
    11341 ccgaacaata aaatgctcga tcgcgacgat atgatccaca ttggtatctg cgcctggcgt
    11401 agtggtcagg ataccgagca ggtaatggaa catgcagagt ctgccacgcg taatgcggga
    11461 ttgcagggcg gcaatagctg ggctatttac gatgactcgt tgcctgaaaa aggacgcggt
    11521 aatgttcgct ggcgtacgct tatcgagcaa atgctcagtc gcggcggccc gcgcctttat
    11581 caaaaaccgg cggttactcg cgaaggtcag gttcatcatc gcgaactcat gtgccgcatc
    11641 ttcgatggta atgaagaggt tagctcggcg gagtatatgc cgatggtctt gcagtttggc
    11701 ttatcggaag agtatgaccg tctgcaaatc agccgtctta ttccactatt gcgttactgg
    11761 ccagaggaaa atctggcgat tcaggttacc gttgagtcgc tgattcgccc gcgttttcag
    11821 cgttggctgc gcgatacgtt aatgcaatgt gaaaaatcac aacgaaaacg cataattatt
    11881 gaacttgcag aggccgatgt aggtcaacat atcagtcgtt tacaacctgt tattcgttta
    11941 gtgaatgctt taggggtacg ggtagccgtc aaccaggctg gtttgacgct ggtaagcacc
    12001 agttggatca aagaacttaa tgttgagtta ctcaagctcc atccggggct ggtcagaaac
    12061 attgagaagc gaacggagaa ccagctgctg gttcaaagcc tggtggaagc ctgctccggg
    12121 accagcaccc acgtttacgc caccggcgtg cgttcgcgaa gcgagtggca gaccctgatt
    12181 cagcgcggtg ttacaggcgg gcaaggggat tttttcgcgt cctcacagcc acttgatact
    12241 aacgtgaaaa aatattcaca aagatactcg gtttaacctg ccgtttaatc cgttttcacg
    12301 tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag attgttgcag
    12361 cacatatgca gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc tgctgatgca
    12421 ttaagccttt ctggactcag gcagagattt gtaacaaagg aaacgaactg cactaatttt
    12481 caccgtagca gatgattttt gcgccttgtc gctgctgcgt gtggttggta aagtaagcgg
    12541 attttctttt ccgccccagc tttcaggatt atcccttagt atgttgaaaa aatttcgtgg
    12601 catgttttcc aatgacttgt ccattgacct gggtactgcg aataccctca tttatgtaaa
    12661 aggacaaggc atcgtattga atgagccttc cgtggtggcc attcgtcagg atcgtgccgg
    12721 ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag cagatgctgg gccgtacgcc
    12781 gggcaatatt gctgccattc gcccaatgaa agacggcgtt atcgccgact tcttcgtgac
    12841 tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc aacagcttta tgcgtccaag
    12901 cccgcgcgtt ctggtttgtg taccggttgg cgcgacccag gttgaacgcc gcgcaattcg
    12961 tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg attgaagaac cgatggctgc
    13021 cgcaattggt gctggcctgc cggtttctga agcgaccggt tctatggtgg ttgatatcgg
    13081 tggtggtacc actgaagttg ctgttatctc cttgaacggc gtggtttact cctcttccgt
    13141 gcgcattggt ggtgatcgtt tcgacgaagc tatcatcaac tatgtgcgtc gtaattacgg
    13201 ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt cggcttaccc
    13261 gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac ctggcagaag gtgttccacg
    13321 cggttttacc ctgaactcca acgaaatcct cgaagcactg caggaaccgc tgaccggtat
    13381 tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg gaactggctt ccgacatctc
    13441 cgagcgcggc atggtgctca ccggtggtgg cgcactgctg cgtaaccttg accgtttgtt
    13501 aatggaagaa accggcattc cagtagttgt tgctgaagac ccgctgacct gtgtggcgcg
    13561 cggtggcggc aaagcgctgg aaatgatcga catgcacggc ggcgacctgt tcagcgaaga
    13621 gtaatcggat gcaggcaggg gaaatgtctg tttaccctgc ctggtctgat acgagaatac
    13681 gcataactta tgaagccaat ttttagccgt ggcccgtcgc tacagattcg ccttattctg
    13741 gcggtgctgg tggcgctcgg cattattatt gccgacagcc gcctggggac gttcagtcaa
    13801 atccgtactt atatggatac cgccgtcagt cctttctact ttgtttccaa tgctcctcgt
    13861 gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg accaactaga acttgaaaac
    13921 cgggcgttac gtcaggaact gttgctgaaa aacagtgaac tgctgatgct tggacaatac
    13981 aaacaggaga acgcgcgtct gcgcgagctg ctgggttccc cgctgcgtca ggatgagcag
    14041 aaaatggtga ctcaggttat ctccacggtt aacgatcctt atagcgatca agttgttatc
    14101 gataaaggta gcgttaatgg cgtttatgaa ggccagccgg tcatcagcga caaaggtgtt
    14161 gttggtcagg tggtggccgt cgctaaactg accagtcgcg tgctgctgat ttgtgatgcg
    14221 acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc agccggtaac
    14281 ggttgtacgg atgatttgca gcttgagcat ctgccagcga atacggatat tcgtgttggt
    14341 gatgtgctgg tgacctccgg tctgggcggt cgtttcccgg aaggctatcc ggtcgcggtt
    14401 gtctcttccg taaaactcga tacccagcgc gcttatactg tgattcaggc gcgtccgact
    14461 gcaggactgc aacgtttgcg ttatctgctg ctgctgtggg gcgcggatcg taacggcgct
    14521 aacccgatga cgccggaaga ggtgcatcgt gttgctaatg aacgtctgat gcagatgatg
    14581 ccgcaggtat tgccttcgcc agacgcgatg gggccaaagt tacctgaacc ggcaacgggg
    14641 atcgctcagc cgactccgca gcaaccgacg acaggaaatg cagctactgc gcctgctgcg
    14701 cccacacagc ctgctgctaa tcgctctcca caaagggcta cgccgccgca aagtggtgct
    14761 caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag ctatcgtagc cagggacgtt
    14821 gggtaatctg gctctctttc ctcattgcgc tgttgctgca aatcatgccc tggccggata
    14881 acctgattgt tttccggcca aactgggtgt tactcatctt gttgtattgg atcctggcct
    14941 tgcctcatcg cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg gatctgatca
    15001 gcggctcgac gcttggcgta cgcgtattgg cgatgagcat cattgcttac ctggtggcgc
    15061 tgaaatacca gcttttccgc aacctcgcat tatggcagca ggcgctggtc gtcatgttgc
    15121 tttcgctggt ggtggatatt attgttttct gggcagagtt tttagtgatt aacgtctctt
    15181 tcagaccgga agtgttctgg agtagtgtag tcaatggggt gctctggccg tggattttct
    15241 tgctgatgcg taaagtccgt cagcagtttg cagtgcaata aaggtttcta tgacttctct
    15301 gtatttagct tccggttctc cgcgtcgtca ggagttactt gcgcaacttg gtgtgacctt
    15361 tgaacgtatt gttacgggca ttgaggagca gcgtcagccg caggagagcg cgcagcagta
    15421 tgttgtgcgt ctggcgcgcg agaaagcaca ggcgggtgtc gcgcaaacgg cgcaggatct
    15481 cccggtgctg ggtgcggata ctatcgttat cctgaatgga gaagtgctgg agaaaccgcg
    15541 cgacgcagag catgcggcgc agatgttgcg caaattatcg ggtcagaccc atcaggtgat
    15601 gacggcagtg gcgttggccg acagccagca cattctcgat tgcctggtgg tcaccgatgt
    15661 gactttcaga acgttaacag acgaagacat cgcgggctat gtcgccagcg gtgaaccgtt
    15721 agataaagca ggtgcatacg gtattcaggg gctgggtggc tgttttgtca ggaagataaa
    15781 tggcagctat cacgccgtag tcggcttacc tctggttgaa acgtatgaat tattaagtaa
    15841 ttttaacgca ctgcgtgaga aaagggataa acatgacggc tgaattgtta gtaaacgtaa
    15901 cgccttcgga aacgcgagtg gcgtatattg atggcggtat tctgcaggaa attcatattg
    15961 aacgtgaggc gcgacgcgga atagtaggca atatctacaa gggtcgtgta agtcgtgtac
    16021 ttccgggtat gcaggcggct tttgtagata ttgggctgga taaagccgcg tttcttcatg
    16081 catccgatat catgccgcac accgaatgtg tggcgggtga agaacaaaag caattcacgg
    16141 tgcgcgacat ctcggaactg gttcgtcagg ggcaagatct gatggtgcag gtggtgaaag
    16201 atccgcttgg cactaaaggt gcgcgcctga ccaccgatat cacgctgcct tctcgctatc
    16261 tggtctttat gccaggggct tctcacgttg gggtttccca acgtattgaa agcgaatcag
    16321 aacgtgaacg cctgaaaaaa gtggtcgcag agtattgcga cgagcagggc gggtttatca
    16381 tccgtaccgc agcggaaggg gttggcgagg ctgaactggc ctccgatgcc gcttatctga
    16441 aacgcgtctg gaccaaagtt atggagcgta aaaagcgccc gcagacccgt tatcagctgt
    16501 acggcgaact ggcgctggct cagcgtgttc tgcgtgattt cgccgacgcc gaactggacc
    16561 gcattcgcgt tgactcacgc ctgacttacg aagcattact tgagttcacc tcggagtaca
    16621 ttcccgagat gaccagcaag ctggagcatt acaccggacg ccagccgatt ttcgatctct
    16681 ttgatgtcga aaacgaaatc cagcgagcgc tggaacgcaa agtagaactg aaatccggtg
    16741 gttatctcat tatcgaccag accgaagcga tgaccaccgt ggacatcaat accggagcgt
    16801 ttgtcggtca tcgcaatctg gacgacacca ttttcaatac caatattgaa gcgacgcagg
    16861 ctatcgctcg ccagttacgg ttgcgtaatc tgggcgggat tatcattatt gatttcatcg
    16921 atatgaataa tgaagatcac cgccgccgcg tgctgcactc gctggagcag gcgttgagca
    16981 aagaccgggt gaaaaccagc gttaatggtt tttcggcgct ggggctggtg gagatgacgc
    17041 gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca acctgccacg
    17101 gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc gagattgttc
    17161 gtgtccacca tgcttacgac tccgaccgtt tcctggtcta tgcttctccg gcagtagccg
    17221 aagccttgaa aggcgaagag tcacactcgc tggcggaagt ggaaattttc gttggcaaac
    17281 aggttaaagt acaaattgaa ccgctctata accaggagca gtttgacgtc gtaatgatgt
    17341 aaacagatgc tgggccgcca tccggcaaag ggtttttgag tcacattttt agcagacaag
    17401 gagtgacggg tgaggcgatt gccggggatt ttactgctta ctggagccgc gctcgttgtg
    17461 atcgctgccc tgctggttag cggcctgcgt attgctttac cgcatcttga cgcctggcgt
    17521 ccggaaatcc tcaacaaaat agaatccgcg actggcatgc cggtagaagc cagtcagctc
    17581 tcagccagct ggcagaattt tggcccgacg cttgaagcac acgacatccg tgcagaacta
    17641 aaagatggcg gcgaattttc ggttaaacgc gttactctgg cgctggatgt ctggcagagc
    17701 ctgttacata tgcgctggca gtttcgcgac ctcactttct ggcagctgcg ctttcgcacc
    17761 aacactccta tcaccagcgg tggtagcgat gacagtctgg aagccagtca catcagcgat
    17821 ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag tttcctgacg
    17881 ccatccggtc agcgtgccga gctggcgatc ccacaactca cctggctgaa cgatccacgt
    17941 cgacaccgtg cggaaggcct ggtaagcctc tccagcctta ccggacagca cggcgtgatg
    18001 caggtgcgca tggatttgcg cgatgatgag gggttgttaa gcaatggtcg cgtctggcta
    18061 caggcggatg acatcgacct gaagccgtgg ctcggtaaat ggatgcagga caatattgct
    18121 ctggaaacgg cacagttctc ccttgaaggc tggatgacga tcgacaaagg cgatgtaacc
    18181 ggcggtgacg tctggctgaa acagggcggt gccagctggt tgggcgagaa gcaaacgcat
    18241 acgctgtcgg tggataatct gaccgcgcat attacgcgtg aaaatccggg ctggcagttc
    18301 tctattcccg atacacggat cacgatggac ggcaaaccct ggccgagcgg agcattgacg
    18361 ctggcctgga taccggaaca ggacgttggc ggcaaagaca ataaacgcag tgacgaactc
    18421 cggattcgcg ccagtaatct ggagctggca ggcctggagg gcatacgccc gctggccgcg
    18481 aaactttcac ctgcactggg tgatgtttgg cgctccacac aaccgagcgg caagattaac
    18541 actctggcgc tggatatccc gcttcaggcg gcagacaaga cccgttttca ggcatcgtgg
    18601 agcgatctgg cctggaagca atggaaatta ttaccgggtg cggaacactt ctccgggacg
    18661 ctttccggca gcgttgaaaa tggtttgctt accgcgtcga tgaagcaggc aaagatgcct
    18721 tacgaaacgg tattccgtgc gccactggaa atcgccgacg gccaggcaac tataagctgg
    18781 ctgaacaatg acaaaggttt ccagctggat gggcgtaata ttgacgttaa agccaaagcc
    18841 gtccatgcgc gcggcggttt tcgttacctg caacctgcta acgatgaacc ctggctgggt
    18901 attctggctg gcatcagtac cgatgatggt tcacaagcct ggcactattt cccggaaaac
    18961 ttgatgggta aagacctggt tgattactta agtggcgcga ttcagggcgg tgaagcggat
    19021 aacgcgacgc tggtttatgg tggcaatccg caactcttcc cctataaaca caacgaaggt
    19081 cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg ccttccagcc ggactggcct
    19141 gcattaacta accttgatat tgaactggac tttattaacg acggtttatg gatgaaaacc
    19201 gatggcgtta atctgggcgg cgtgcgcgcg agtaatctca ccgcagtgat ccctgactac
    19261 tcgaaagaaa aactgctgat tgacgctgac attaaaggtc cgggtaaagc cgttggccct
    19321 tactttgatg agacaccgct gaaagattct ctgggtgcga ccctgcaaga actccagctc
    19381 gacggcgatg tgaatgctcg cttacatctt gatatcccgc tgaacggcga gctggtaacc
    19441 gcgaaaggtg aagtgacgct gcgtaataac agtctgttta tcaaaccact cgacagcacc
    19501 ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg atctgcaaag tgaaccactg
    19561 acagcaagct ggtttaatca gccgttgaac gtggattttt ccaccaaaga aggggcaaaa
    19621 gcctaccagg ttgcggtgaa tctcaacggt aactggcaac cggcgaaaac cggcgttctg
    19681 cctgaagcgg tgaacgaagc attgagtggc agcgtggcgt gggatggtaa agtgggcatt
    19741 gatctgcctt atcatgctgg cgcgacctat aacgtagagc tgaacggcga tctgaataat
    19801 gtgagtagtc acttaccttc accgttagcc aaacctgcgg gtgaaccact agcggtaaac
    19861 gttaaggttg atggcaatct caacagcttt gaattaaccg gacaggctgg tgcggataac
    19921 catttcaata gccgctggtt gcttggtcaa aagctgacgc tcgatcgtgc tatttgggcg
    19981 gcagacagta aaacgctccc gccgttgccg gaacaaagtg gcgttgaact caatatgccg
    20041 ccgatgaatg gtgccgagtg gctggccctg ttccagaaag gcgctgcgga gagtgtcggt
    20101 ggtgcagcga gtttcccaca atacataacg ttacgtacgc ctatgttgtc actgggaaat
    20161 cagcaatgga ataacctgag tattgtttcg caaccgacgg caaatggcac cctggttgag
    20221 gcgcaagggc gtgaaatcaa cgccacgcta gcgatgcgta ataacgcgcc gtggctggcg
    20281 aatatcaaat atctttatta caacccgagc gtggcgaaaa ctcgtggtga ttcaacgccg
    20341 tcatcacctt tcccgacaac ggagcgcatt aacttccgtg gctggccgga cgcacaaata
    20401 cgatgcacag agtgctggtt ctgggggcaa aaattcggtc gcattgacag tgatatcacc
    20461 atttctggcg atacgttaac gctgaccaat ggactgattg atactggttt ctcgcggctt
    20521 actgccgatg gtgaatgggt taataatccg gggaatgaac gtacctcgct gaaaggaaaa
    20581 ctgcgcgggc agaaaattga tgccgccgca gaattttttg gtgtcacgac gcccatacgc
    20641 cagtcgtcat ttaatgtgga ttacgattta cactggcgca aagcaccctg gcagccagat
    20701 gaagcgacgt tgaatggcat cattcatact caactgggta aaggcgaaat taccgaaatc
    20761 aataccggac atgccgggca attgctgcgc ttattgagcg tagatgccct gatgcgtaag
    20821 ctgcgttttg atttcagaga cacttttggc gaagggttct attttgactc cattcgcagc
    20881 accgcgtgga ttaaagacgg ggttatgcac accgacgaca cgctggtgga tggcctggag
    20941 gcggatatcg ccatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa tatggaagcg
    21001 gttgtcgcac cagagatttc tgcgacggtg ggcgtggctg cggcttttgc ggttaacccc
    21061 attgttggcg cggcagtttt tgccgctagt aaagtgctgg ggccgctgtg gagcaaagtc
    21121 tccattttgc gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa cgaagtgttg
    21181 cgccaaccgc gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt gccccaatct
    21241 catatgataa tcgttgccaa aggccaacga gccagaacat aaccgtaggc cggatagggc
    21301 gttcatgccg catccggcag ccgtaaaaaa tcctctactg cagtaactaa cgagtagcaa
    21361 aaacgatgag tctgaacctg gtaagtgaac aattgctagc ggcgaacagc ctgaaacatc
    21421 aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg ccttgattat ggcgatctct
    21481 attttcagtc gagctatcac gaatcctggg ttttagaaga ccgcattatt aaagatggtt
    21541 cttacaacat cgatcagggc gttggcgtgc gtgcaatcag cggtgaaaaa accggatttg
    21601 cttacgctga ccaaatcagc ctgctggcgc tggaacagag tgcgcaagcg gcgcgcacca
    21661 tcgtccgtga tagtggtgat ggcaaagtac agacgctggg cgcggtagag catagcccgt
    21721 tgtatacctc ggtagatccg ctgcaaagca tgagccgtga agagaagctg gatatcctgc
    21781 gtcgcgtcga taaggttgct cgcgaagcgg acaagcgcgt acaggaagtg actgccagcc
    21841 tcagtggtgt ctatgaatta attctggttg cggccaccga cggcacgcta gcggcggatg
    21901 tccgtccgct ggtgcgtctt tccgtgagcg ttctggtcga agaagatggc aaacgcgaac
    21961 gcggtgccag tggcggcggc ggtcgttttg gttatgagtt cttccttgcc gatcttgacg
    22021 gcgaagtccg tgcggatgca tgggcaaaag aagcagtgcg tatggcgctg gtcaatcttt
    22081 ctgccgttgc tgcaccagcg ggcaccatgc cggtagtact tggcgcaggt tggccgggcg
    22141 tgctgttgca tgaagcggta ggtcacggtc tggaaggcga cttcaaccgc cgcggcactt
    22201 cagtatttag tgggcaggtc ggggagctgg tggcttcaga actgtgtacc gtggttgatg
    22261 acggcacgat ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt acgccaggcc
    22321 agtacaacgt gctgattgag aacggcattc tgaaaggcta catgcaggat aaactcaacg
    22381 cgcgtttgat ggggatgacg ccgactggca acggtcgccg tgaatcctac gcccatctgc
    22441 ccatgccgcg tatgactaac acctatatgc tgccgggtaa atcgaccccg caggaaatta
    22501 ttgaatccgt tgagtacggt atctatgcac cgaactttgg tggcggtcag gtggatatca
    22561 cctccggcaa attcgttttc tccacttcag aagcatatct gattgaaaac ggtaaagtaa
    22621 cgaagccggt gaaaggcgca acgttgattg gttccggtat cgaaaccatg cagcagattt
    22681 cgatggttgg caacgacctg aaactggata acggcgtggg tgtctgcggt aaagaagggc
    22741 aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt tgataacctg actgttggcg
    22801 gtactgcgta ataatttcat tattttcaga aggataatta atctttctac gtgcacgaac
    22861 ggtcccctcg ctcctctggg gttagggtga ggggaacccg ttggcacagg tttgtagaac
    22921 gtaacagtac aatatgaatt acttctcttt cccgcgcccg tgcatctcct gaaacaattt
    22981 accgacctca acaaaataat ccgtcagcga gttgatcacg acctgtacct tcagcggcag
    23041 cttatctttt tcggtatata acgcataaac cgggcgtgga tctgactgat aacgcggcag
    23101 caggatctcc agctcaccgc gattgatctc gttgatcacc cacatcagcg gcacgtaggc
    23161 gatcccggca cccgccgtca gccagcgcac cagcgtcatc ggatcattag tcacaaatct
    23221 tccttgtggg atcaggcgag tcgagatccc ttccggtgcg atcagttcaa attcattgtc
    23281 gggccgcacg ctgtattcaa gccatgaatg actactcaaa tcggcgggtt tttccggtat
    23341 gccgtattgt gtgagatagc ttttcgcggc gcacaccacc atcggcatcg cgcccagacg
    23401 gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg atcaccacat ccagaccgtc
    23461 ggcaatcagg tcgggggctg gaattccggt aaccagattg acgctcaaac ctgggtattc
    23521 tttcagcatt tttgctgtca gcccggcgag aacattttgt gccatagttg aagaacagcc
    23581 aatgcgcagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat gaacatcctg
    23641 cacttcatga agcatccgac ggcagccctg gtagtaaatt ctgccggctt cggtcaggcc
    23701 aatgctgcgt gtgctacggt ttaacagctt tacctgcaac tcatcttcca gttttgacac
    23761 cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg taaaagagcc
    23821 aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta ttcactctga
    23881 cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg ctaatatatt
    23941 aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc tctgcggcga
    24001 cagatgctga aaataataac gcctgctctc tctttacaac caaggtcaac atgagtctgt
    24061 ttcccgttat cgtggtgttt ggactgtcct tcccaccgat attttttgaa ttgcttttat
    24121 cactggcgat tttctggctg gtgcgccggg tacttgtgcc aacaggtatc tacgactttg
    24181 tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat ttgatatcgc
    24241 gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg tacggccatc
    24301 acggtcgtat tagtcattct agccttcatc gcaattttta atgcctgggt ctattacacc
    24361 gaatccccct ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat cgcgccggac
    24421 gtttctggac tcattaccca ggtgaatgtt catgataacc agctggtgaa aaaaggacag
    24481 atactgttca ccatcgacca gccgcgctat caaaaggcgc ttgaggaagc gcaagccgat
    24541 gttgcttatt atcaggtact ggcacaggag aaacgccagg aggccggacg tcgtaaccgt
    24601 ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg ccaacaacgt actacaaacg
    24661 gttctgcatc agttagcgaa agcgcaggcg acccgcgatc tggcaaaact ggatcttgaa
    24721 cgcacggtga tccgcgcgcc agcagatggc tgggtgacca acctcaacgt ctataccggt
    24781 gagtttatta ctcgaggatc aacggcggtt gcgctggtga aacagaactc cttctatgta
    24841 ctggcctata tggaagaaac taagctggaa ggggggcgtc cggggtatcg tgcagagatc
    24901 acgccgcttg gcagtaacaa agtgctgaaa gggactgttg atagtgttgc cgcaggggtc
    24961 accaacgcca gcagcacgcg tgacgacaaa gggatggcga ctatagactc taatcttgaa
    25021 tgggtgcgtc ttgcgcaacg tgttccggtt cgtattcgtc tcgacaacca gcaagagaac
    25081 atctggcctg cgggcaccac tgctacagtg gtggtcactg gcaaacaaga tcgcgacgaa
    25141 agccaggatt cgttcttccg taaaatggcc catcgcctgc gtgagtttgg ttaatcacga
    25201 tgggtatttt ctccattgct aaccaacata ttcgctttgc ggtaaaactg gcgaccgcca
    25261 ttgtactggc gctgtttgtt ggctttcact tccagctgga aacgccacgc tgggcggtac
    25321 tgacagcggc gattgttgcc gccggtccgg cctttgctgc gggaggtgaa ccgtattctg
    25381 gcgctattcg ctatcgtggc tttttgcgca tcatcggcac atttattggc tgtattgccg
    25441 gactggtgat catcattgcg atgatccgcg caccattatt gatgattctg gtgtgctgta
    25501 tctgggccgg tttttgtacc tggatatcct cgctggtacg aatagaaaac tcgtacgcgt
    25561 gggggctggc cggttatacc gcgctgatca ttgtgatcac cattcagccg gaaccattgc
    25621 ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt gtgtgtgcga
    25681 ttatggcgga tttgctcttt tctccgcgat cgatcaaaca agaagtagat cgagagctgg
    25741 aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg tatcaagcat ggcgatggtg
    25801 aagttgtcga taaagcctgg ggcgacctgg tgcgacgcac cacggcacta caaggcatgc
    25861 gcagcaacct gaatatggaa tcttcccgct gggcgcgggc caatcgacgt ttaaaagcga
    25921 tcaatacgct atcgctgacg ctgattaccc aatcctgcga aacttatctt attcagaata
    25981 cgcgcccgga attgatcact gatactttcc gcgaattttt tgacacgccg gtagaaaccg
    26041 cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt tatcgcctgg accggggaac
    26101 gggaaacgcc tgtcaccatt tatagctggg tcgcggcggc aacgcgttat cagcttctca
    26161 agcgcggcgt tatcagtaac acaaaaatca acgccaccga agaagagatc ctgcaaggcg
    26221 aaccggaagt aaaagtagag tcagccgaac gtcatcatgc aatggttaac ttctggcgaa
    26281 ccacactttc ctgcattctg ggcacgcttt tctggctgtg gacgggctgg acttccggca
    26341 gtggtgcaat ggtgatgatt gcggtcgtga cgtcactggc aatgcgtttg ccgaatccac
    26401 gcatggtggc gatcgacttt atctatggga cgctggccgc gctgccgtta gggctgctct
    26461 actttttggt gattatccct aatacccaac agagcatgtt gctgctgtgt attagcctgg
    26521 cagtgctggg attcttcctc ggtatagaag tacagaaacg gcgactgggc tcgatggggg
    26581 cactggccag caccataaat attatcgtgc tggataaccc gatgactttc catttcagtc
    26641 agtttctcga cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc accgttattt
    26701 tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt actgcttaat cagtttgttt
    26761 ttgccgctgt ttccgcgatg actaccaatg tggcacgtcg taaagagaac cacctcccgg
    26821 cactttatca gcagctgttt ttgctgatga ataagttccc aggggatttg ccgaaatttc
    26881 gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg atcccggtta
    26941 acgaggattt atcggcgttt caccgacaaa tgcgccgcac agcagaccat gtgatatctg
    27001 cccgtagcga tgataaacgt cgtcggtact ttggccagtt gctggaagaa ctggaaatct
    27061 accaggaaaa gctacgcatc tggcaagcgc caccgcaggt gacggaaccg gttcatcggc
    27121 tggcggggat gctccataag tatcaacatg cgttgaccga tagttaagtc aaaaccgacg
    27181 ccaaaagcgt cggttttttc atggctatac ttagcgatgc acggcagaac tcgccgcgaa
    27241 acgtgacggt ggcaacagat gaatatttat acctttgatt ttgatgagat tgagagtcag
    27301 gaggattttt atcgtgactt tagccaaacc tttggtctgg ctaaagataa ggtacgcgat
    27361 ctcgactcac tatgggatgt gttaatgaac gatgtcctgc cgctaccact tgagattgaa
    27421 tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt gctgtttgat
    27481 gaggcagagg aagagctgga agggcatttg cgttttaatg ttcgtcatta gcgcaaaaaa
    27541 aagcccccga accgggggca atatcgtcgg acaagacgat gagggtttat ttgtacagtt
    27601 cagccgtagc gtgccaggtg tcaccgctac gagcttcagt aatctggtag gccgttgcgc
    27661 ctttctcttc cgcttttttg ttcagcattt cacgcatatc cattggcgaa gacgccacac
    27721 cacttacgga tacggtcccg attgcttcac gattttgtgc ttgtgcagta tcaatggagt
    27781 cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct taatgcagct acagtggttt
    27841 tgattttcat gatttttacc tcgacataat cttttagctg ggcctttgtt tcgtgaccca
    27901 tatcacaaaa tcaagcatac actaattatt gaactaatta ata
//
LOCUS       DAZPJP010000037        27468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPJP010000037 DAZPJP010000000
VERSION     DAZPJP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.037"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHU9J_001463"
     CDS             complement(<1..81)
                     /locus_tag="ACHU9J_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1905047.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHU9J_001464"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHU9J_001464"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1905048.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHU9J_001465"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHU9J_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1905049.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHU9J_001466"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHU9J_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1905050.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCIGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHU9J_001467"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHU9J_001467"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1905051.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHU9J_001468"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHU9J_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1905052.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHU9J_001469"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHU9J_001469"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1905053.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHU9J_001470"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHU9J_001470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1905054.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHU9J_001471"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHU9J_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHU9J_001472"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHU9J_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1905055.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHU9J_001473"
     CDS             complement(12387..12626)
                     /locus_tag="ACHU9J_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1905056.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHU9J_001474"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHU9J_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1905057.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHU9J_001475"
     CDS             13003..13290
                     /locus_tag="ACHU9J_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1905058.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHU9J_001476"
     CDS             13960..14103
                     /locus_tag="ACHU9J_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1905059.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHU9J_001477"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHU9J_001477"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1905060.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHU9J_001478"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHU9J_001478"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1905061.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHU9J_001479"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHU9J_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1905062.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="ACHU9J_001480"
     CDS             complement(16292..16750)
                     /locus_tag="ACHU9J_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1905063.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHU9J_001481"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHU9J_001481"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1905064.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHU9J_001482"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHU9J_001482"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1905065.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHU9J_001483"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHU9J_001483"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1905066.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHU9J_001484"
     CDS             19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHU9J_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1905067.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHU9J_001485"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHU9J_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1905068.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="ACHU9J_001486"
     CDS             complement(21526..23124)
                     /locus_tag="ACHU9J_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1905069.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHU9J_001487"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHU9J_001487"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1905070.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="ACHU9J_001488"
     CDS             complement(24803..25381)
                     /locus_tag="ACHU9J_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1905071.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHU9J_001489"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHU9J_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1905072.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="ACHU9J_001490"
     CDS             26820..26999
                     /locus_tag="ACHU9J_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1905073.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27418)
                     /locus_tag="ACHU9J_001491"
     CDS             complement(27119..27418)
                     /locus_tag="ACHU9J_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1905074.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
BASE COUNT         7066 a         6773 c         6940 g         6689 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaatacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttatcgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacct
//
LOCUS       DAZPJP010000038        27297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPJP010000038 DAZPJP010000000
VERSION     DAZPJP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.038"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            156..713
                     /gene="pinE"
                     /locus_tag="ACHU9J_001492"
                     /pseudo
     CDS             156..713
                     /gene="pinE"
                     /locus_tag="ACHU9J_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(1052..1900)
                     /locus_tag="ACHU9J_001493"
     CDS             complement(1052..1900)
                     /locus_tag="ACHU9J_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1905075.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            2109..2744
                     /gene="yfhb"
                     /locus_tag="ACHU9J_001494"
     CDS             2109..2744
                     /gene="yfhb"
                     /locus_tag="ACHU9J_001494"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1905076.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2802..3305
                     /gene="tadA"
                     /locus_tag="ACHU9J_001495"
     CDS             2802..3305
                     /gene="tadA"
                     /locus_tag="ACHU9J_001495"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1905077.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(3302..4858)
                     /gene="mltF"
                     /locus_tag="ACHU9J_001496"
     CDS             complement(3302..4858)
                     /gene="mltF"
                     /locus_tag="ACHU9J_001496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1905078.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            5116..9003
                     /gene="purL"
                     /locus_tag="ACHU9J_001497"
                     /gene_synonym="purI"
     CDS             5116..9003
                     /gene="purL"
                     /locus_tag="ACHU9J_001497"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1905079.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            9561..10988
                     /gene="qseE"
                     /locus_tag="ACHU9J_001498"
     CDS             9561..10988
                     /gene="qseE"
                     /locus_tag="ACHU9J_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1905080.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            11153..11866
                     /gene="qseG"
                     /locus_tag="ACHU9J_001499"
     CDS             11153..11866
                     /gene="qseG"
                     /locus_tag="ACHU9J_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1905081.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            11856..13190
                     /gene="glrR"
                     /locus_tag="ACHU9J_001500"
     CDS             11856..13190
                     /gene="glrR"
                     /locus_tag="ACHU9J_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1905082.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            13251..13589
                     /gene="glnB"
                     /locus_tag="ACHU9J_001501"
     CDS             13251..13589
                     /gene="glnB"
                     /locus_tag="ACHU9J_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1905083.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13634..14824)
                     /gene="hmpA"
                     /locus_tag="ACHU9J_001502"
     CDS             complement(13634..14824)
                     /gene="hmpA"
                     /locus_tag="ACHU9J_001502"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1905084.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            15152..16405
                     /gene="glyA"
                     /locus_tag="ACHU9J_001503"
     CDS             15152..16405
                     /gene="glyA"
                     /locus_tag="ACHU9J_001503"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1905085.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(16603..17796)
                     /locus_tag="ACHU9J_001504"
                     /pseudo
     CDS             complement(16603..17796)
                     /locus_tag="ACHU9J_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            17914..21191
                     /locus_tag="ACHU9J_001505"
                     /pseudo
     CDS             17914..21191
                     /locus_tag="ACHU9J_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            21288..22271
                     /locus_tag="ACHU9J_001506"
     CDS             21288..22271
                     /locus_tag="ACHU9J_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1905086.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            22294..23805
                     /locus_tag="ACHU9J_001507"
     CDS             22294..23805
                     /locus_tag="ACHU9J_001507"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1905087.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            23830..24828
                     /locus_tag="ACHU9J_001508"
     CDS             23830..24828
                     /locus_tag="ACHU9J_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1905088.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24861..25955
                     /locus_tag="ACHU9J_001509"
     CDS             24861..25955
                     /locus_tag="ACHU9J_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1905089.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25967..26839
                     /locus_tag="ACHU9J_001510"
     CDS             25967..26839
                     /locus_tag="ACHU9J_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1905090.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(26887..>27297)
                     /locus_tag="ACHU9J_001511"
     CDS             complement(26887..>27297)
                     /locus_tag="ACHU9J_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1905091.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6404 a         6870 c         7494 g         6529 t
ORIGIN      
        1 tagtaatgat ccaaatcata tctcaatgta ctgttttttt ctttaaaaaa acagtacatt
       61 ctactttata atgactgtgt attcccatgg aaggagatca cccaattctc ccccccgact
      121 tttgacgcag tgtcttgtta acagggggat ttaccatgct tattggctat gtacgcgtat
      181 caacaaatga ccagaatacc gatctacaac gtaatgcgtt gaactgtgca ggatgcgagc
      241 tgatttttga agacaagata agtggcacaa agtccgaaag gccggggctg aaaaaactgc
      301 tcaggacatt atcggcaggc gacacactgg ttgtctggaa gctggatcgg ctggggcgca
      361 gtatgcggca tcttgtcgtg ctggtggagg agttgcgcga acgaggcatc aactttcgta
      421 gtctgactga ttcaattgat accagcacac caatgggacg ctttttcttc catgtgatgg
      481 gtgccctggc tgaaatggaa cgtgaactga ttgttgaacg aacaaaagca ggactggaag
      541 ctgctcgcgc acagggacga attggcggtc gccgtcccaa actttcacca gaataatggg
      601 cgcaggccgg gcgactaatt gcatcaggag tttctcgcca gaaggtggcg atcatctatg
      661 atgttggcga atcgactttt ttgtataaga ggtttccagc cagggttata taaaaccgca
      721 gacacggcgt atacaagaac gtgctacggt tgactgaaaa gttaatgtgg tatgtcacag
      781 gccttttgcg ccgtaaagcc ccgtccttta gggcgcggag gacgttaaag aaagtggtgt
      841 tctttgtgtt gctgaactta tgcaaaattt ttttgcacca taaatccaac aacacattca
      901 ataacacatt gctgtatcac tatgaaaata aatgtgtttt tatttgattt cacacatatc
      961 acaattgatg cattttttag tgattaacaa cgccataagg aaactctcaa aacaacacaa
     1021 accgggcggg cattgtacgc gcattcttta ctcagaccag ttttttcacc agcgcttcac
     1081 tatgacgaat acgttctggt gccagttcca gatcctgctg aatcagcgca atgaacagca
     1141 aatccgttaa cattccctga gcgtgacaag cagagattga agcactgttt gtcgcttgtt
     1201 cttcggcaat ggtatacagg caatgagaag cacgctgttg cagggcattc ggagtaaagc
     1261 cggtaatcgc cagcactttt ccgcccactc gcagcatctc atctgccgcc aggtttaact
     1321 cgcgtcgtac accggtgtag gaaatggcta ataacagatc gtcaggggac gacgcctgta
     1381 ctgttgcgag tagcgcatgc atatcgcgca ctgcggcagc attgaagcca atcttcatca
     1441 gcttccaggc aaagttttgc gccaccagac ccgaagcgcc aataccggtc agaattatcc
     1501 gccgcgcaga gcgcaacatt gttacgcatt catgcagttt ctcttcacta ttaacgttta
     1561 gcgttgcgta catagcggca gtattttctt taatcagttt ttcgccgacc agccgtaacg
     1621 gatcatcacc gcggatttgg ttatgaatgg gcacggaggg cgattccggc tggcttgcca
     1681 gcgcttcact caacgccaac ttaagcgccg gaaaaccttt atagccgagt ttttgcgcga
     1741 acttcacgac gctggactga ctgactccgg cttcgttggc cagttgctga gagcttaaat
     1801 ggcgcgccgt atcaggttgt agcagcagat aatccgccag ttttttatca ctctgggcaa
     1861 gcccctggta acgctgacgg atacgaagta agccgttcat atctttcctc gcgatgaaat
     1921 agtggcgaca acaggcaata tgagacacac gcgagtgaat tttatattcc attgccgcta
     1981 attgccatga attaaaaatt ccagttagcc atgatgcggt cgattgttgc cggaaaagtg
     2041 atgatctatc tgttaggcta tgtagtagcg aaaattaatc aggatgtttc agtccagagg
     2101 agtatggttt ggcaactcac gagcgtcgtg tggtgttttt tgacttagat ggaacattgc
     2161 atcagcagga tatgttcggc agttttctgc gctatttact gcgtcgccaa ccgctgaatg
     2221 cgttacttgt cctgccgttg ttaccgatta tagccattgc gttattgata aaaggtcgtg
     2281 cggcacgctg gccgatgagt ctgcttctgt gggggtgcac ttttggtcac agcgaagcac
     2341 gtttacagac gttgcaggcc gatttcgtgc gctggtttcg cgacaatgtt accgcctttc
     2401 cgctggttca ggagcgatta accacctacc tgttaagttc cgatgctgat atctggttga
     2461 ttaccggctc tccgcagccg ctggttgaag cggtttattt cgatacgccc tggctgccgc
     2521 gggttaatct tatcgccagc caaattcagc gtggctatgg tggttgggta ttgacgatgc
     2581 gttgtctggg acatgaaaag gtcgcacaac tggagcgcaa aatcggcact ccgctgcggc
     2641 tgtacagtgg ctatagcgac agtaatcagg acaatccgct gctttatttc tgtcagcatc
     2701 gttggcgagt aaccccgcgc ggtgaactcc agcaactgga atagagtaaa gcatagcgtc
     2761 cgtgtataat gcgccgcgct tttataaccg gagttttctt tttgtctgaa gtcgaattta
     2821 gccacgaata ctggatgcgt cacgcgctga cgctggcgaa acgtgcctgg gatgagcggg
     2881 aagtgccggt cggcgcggta ttagtgcata acaatcgggt aatcggcgaa ggctggaacc
     2941 gcccgattgg tcgccatgat cccaccgcac atgcagaaat catggccctg cggcagggtg
     3001 gtctggtgat gcaaaattat cgtctgatcg acgccacgtt gtatgtcacg cttgaaccat
     3061 gtgtaatgtg tgccggagcg atgatccaca gtcgcattgg tcgcgtggtc tttggtgcgc
     3121 gtgacgcgaa aactggcgct gcgggatctt taatggatgt gctgcatcat ccgggtatga
     3181 atcaccgagt ggaaattacg gaaggaatac tggcggatga gtgcgcggcg ttgctcagtg
     3241 acttctttcg catgcgccgc caggaaatta aagcgcagaa aaaagcgcaa ccctcgacgg
     3301 attaattttg tttctcttca ctccctttcc tggaaaacag cagagaggga gaatgggtca
     3361 aatagtttga tgacgactgg gaaagaaacg agagaaaagg aaatttctct ttgcccaact
     3421 ccgtaggcga taccgccgga taatcctgcg ccagttgcat cgccgcttct gtagcctgct
     3481 tctctttctc ttgcagataa cccaccaggc taatctgata cttacgaata ttttcgacat
     3541 aagcgtaggc ttcatgtcca cgagcgtagc cgtaagtcag cttgctgtaa tagggtttct
     3601 ggctaagtaa aggcagacgc tgttttacgt cagcccaact gtcaggattc cctttggttt
     3661 ttgccgtcag ggcgcgggca tccagcatat gcgcatagcc catattgtac gcagcgaggg
     3721 caaaccagat ccgctcgttc tccggcacac tttccggcac tttactcatc atatcctgca
     3781 aataacgcac gccaccgctg atgctctgtt cggcatcggt acgatccgta atgccgaggc
     3841 tttgcgcggt atttttggtt aacatcatca tgccgcgcac acccgtcggt gaagtggcct
     3901 gtgcatccca gtgcgattcc tgataagcaa tagcggccag caaacgccag tcaatttctt
     3961 cggcgtattt ctcaaacagg ggctttaact gcggcagtac cgcatcgacg gcgcgtaaaa
     4021 atgtgcgcgt atcgacgtaa tcaaaatcat cgccatgccc caggtatttc tcttcaatgc
     4081 gtgccagcgt acagtcttca ttcatttcgt tgaagaagtc gagcagggcg gcggaaaggg
     4141 tattatcgcc atctaacggg ctaaaccaag tcaccggttg ttcatcggtg atatcgagcg
     4201 ctacggcgag ctccgggtga acgcgctgaa acaggctgat ggcgacagaa tcagcaatgg
     4261 tgtaatcgag ttttccttcg atgacatctt ccattaattc cgcagagcct tttttgtcgt
     4321 ctaccttcca gcttaattcc gggaattttg tgtctttcag ggtttggaga tcgttaacca
     4381 ccacgtgacc cggtgcaacg gtgagttgct ccgccgtcag gttgcccagt gtgcgcgggc
     4441 gatactgact cactttataa accagttgtt gtgacacgga ataataggta gggccaggct
     4501 gataattttt tacccgctca ctgttataga ccagtcctgc cgccagcagg tcggcgttac
     4561 cattatcgag gtcgtcaaac agctggctga tattctgccg cacggtcact ttcagtttta
     4621 caccgaggta atcggcaaac tgtttcgcca gttcgtaatc caggccaaaa ggtttcccgt
     4681 tgatttcgtt ataagtcagg ggagtatgaa tggtgctcac acgcaactct ccccgcgctt
     4741 gaatggcggc gatacggttg tcggctttac caaaccaggg aatggatggc cagagagcga
     4801 ccgcgagcag cagtgccaga atgccgatga acagataatt aatctttaat tttttcaatt
     4861 agttaattct ctgtgtcgtg cgcgtcccag cttgaaaaaa cgtaataata gtgaaaggtt
     4921 tactcataaa tgagcggcat tttgcgtaaa cctgcgccag atggcaactt attacagcca
     4981 ttggcggcac gcgttgctaa ttcacgatgg tgattttatt tccacgcaaa cggtttcgtc
     5041 agcgcatcag attctttata atgacgcccg tttccccccc ttgggtacac cgaaagctta
     5101 gaagacgaga gacttatgat ggaaattctg cgtggttcgc ctgcactgtc ggcattccga
     5161 atcaacaaac tgctggcacg ttttcaggct gccaggctcc cggttcacaa tatttacgcc
     5221 gagtatgtcc attttgctga cctcaatgcg ccgttaaacg atgatgagca cgcacaactt
     5281 gaacgcctgc tgaaatatgg cccggcactc gccagccacg ccccgcaagg caaactcctg
     5341 ctggtgaccc cgcgtcctgg caccatctct ccctggtctt cgaaagcgac cgatattgcc
     5401 cataactgcg ggctacaaca ggtaaaccgc cttgagcgcg gcgttgctta ctatatagaa
     5461 gccggtacgc tgaccaatga acaatggcag caggttaccg ctgaactgca cgaccgcatg
     5521 atggaaacgg tcttttttgc tttagatgat gcagagcagt tgtttgccca ccatcaaccg
     5581 actccggtta ccagcgttga tttgctgggg cagggccgtc aggcgctgat cgacgctaac
     5641 ctgcgtcttg gcctggctct ggcggaagat gaaattgact atctgcagga tgctttcaca
     5701 aagcttggtc gtaacccgaa cgacatcgag ctgtatatgt tcgctcaggc gaactctgag
     5761 cactgccgcc acaaaatttt taatgccgac tggattatcg acggtgaaca gcagccgaaa
     5821 tcgctgttca agatgatcaa aaatactttc gaaaccacgc cagatcacgt tctctctgct
     5881 tataaagata acgccgccgt aatggaaggt tctgaagtgg gccgctactt tgctgaccac
     5941 gaaacgggcc gctacgattt ccatcaggaa ccggcgcata ttctgatgaa agtcgaaact
     6001 cacaaccacc cgacggcgat ttctccgtgg ccgggcgcgg cgacgggttc cggcggcgaa
     6061 attcgtgatg aaggcgccac cggacgcggt gcgaagccga aggctggtct ggttggtttc
     6121 tccgtttcca acctgcgaat tcctggcttc gaacagccgt gggaagaaga tttcggtaag
     6181 cctgagcgca ttgtcaccgc gctggacatc atgaccgaag gcccgctggg cggcgcggcg
     6241 tttaacaacg aatttggtcg tccggcactg aatggctact tccgtactta tgaagaaaaa
     6301 gtgaacagcc acaacggcga agagctgcgc ggttatcaca aaccgatcat gctggcgggc
     6361 gggatcggca acattcgcgc cgatcacgta caaaaaggcg agatcaacgt cggtgcgaag
     6421 ctggtcgttc tcggcggccc ggcaatgaac atcggtcttg gcggcggagc ggcgtcttcc
     6481 atggcgtcag gtcagtctga tgccgacctc gactttgctt ccgtacagcg cgacaacccg
     6541 gagatggagc gtcgctgcca ggaagtgatc gaccgttgct ggcagcttgg tgatgccaac
     6601 ccaatcctgt ttatccacga cgtcggcgct ggcggtcttt ctaacgcgat gccggaactg
     6661 gtgagcgacg gcgggcgcgg cggtaaattt gaactgcgcg atattctaag cgacgaaccg
     6721 ggcatgagcc cgctggaaat ctggtgtaac gaatcccagg aacgctacgt gctggcggtt
     6781 gctgccgatc aattaccgct gtttgacgaa ctgtgtaagc gtgagcgcgc accctacgcg
     6841 gtgattggtg aagcgaccga agaactgcat ctttctctgc acgatcgtca ttttgataat
     6901 cagccgatcg atctgccgct ggacgtcctg cttggtaaaa cgccgaagat gacccgcgat
     6961 gtacaaacgc tgaaagcgaa gggcgatgcg ctggtccgtg aagggatcac cattgcagac
     7021 gcggtgaaac gcgtactgca tctgccgacc gtggcagaga aaaccttcct ggtgaccatc
     7081 ggcgaccgca gcgtaaccgg catggtagcg cgcgatcaga tggtggggcc gtggcaggtg
     7141 ccggtcgcta actgcgcggt cactaccgcc agcctcgaca gctactacgg tgaagcgatg
     7201 gcgattggcg agcgtgcgcc ggttgcactg ctggatttcg ccgcctctgc ccgtctggcg
     7261 gtcggtgaag cgttaaccaa tattgccgca acacagattg gcgatatcaa acgcattaaa
     7321 ctttccgcca actggatggc ggcggcaggc caccctggtg aagatgcggg cctgtatgaa
     7381 gccgttaaag ccgtgggcga agagctttgt ccggcgctgg gcctgacgat cccggtgggt
     7441 aaagactcta tgtcgatgaa aacccgctgg caggaaggta acgaagagcg cgaaatgacg
     7501 tcgccgctgt cgctggtgat ttctgcattt gcccgcgtgg aagatgtgcg tcacaccatc
     7561 acgccacagc tttctaccga agataacaca ctgctgctga ttgatttggg caaaggcaat
     7621 aacgcgctgg gcgcaacggc gctggcgcag gtttatcgtc agcttggcga caaaccggca
     7681 gatgtacgcg acgtcgcgca actgaaaggc ttctatgacg cgattcaggc gctggttgca
     7741 cagcgtaagc tgctcgcata ccacgaccgc tctgatggcg gcctgctggt aacgctggcg
     7801 gaaatggcct ttgctggtca ttgcggcatt aacgcggata ttgcctctct gggcgacgat
     7861 cgcctggcgg cgttgtttaa cgaagaactg ggtgcggtga ttcaggttcg tgccgctgac
     7921 cgtgaagcgg tcgagtccgt actggcacag catgggcttg ctgattgtgt ccattatgta
     7981 gggcaggcgg tttccggtga ccgttttgtg attaccgcca acgggcagac tgtattcagc
     8041 gaaagccgca ccacgttgcg tgtctggtgg gcagaaacta cctggcagat gcagcgcctg
     8101 cgtgacaacc cggagtgtgc tgaccaggag caccaggcga aatcaaacga cgccgatccg
     8161 ggcctgaatg tgaaactgtc gttcgatatc aacgaagatg tggcagcacc gtatattgcc
     8221 actggcgcac gtccgaaagt tgctgtgctg cgtgagcagg gcgtgaactc gcatgttgaa
     8281 atggcggcag ctttccaccg tgcaggcttt gatgctatcg acgtgcatat gagtgacctg
     8341 ctgaccggac gcacgggcct ggaagatttc cacgccctgg tcgcgtgcgg tggtttctcc
     8401 tacggtgatg tgctgggtgc cggtgaaggt tgggcgaagt caatcctgtt caatgaccgt
     8461 gtacgcgatg agtttgcaac cttcttccac cgtccgcaaa cgctggcgct gggggtatgt
     8521 aacggttgcc agatgatgtc taacctgcgt gaactgatcc cggggagcga gttgtggcca
     8581 cgttttgtgc gtaacacctc cgatcgcttt gaagcgcgtt tcagcctggt tgaagtaacc
     8641 caaagcccgt ctctgctgtt gcaggggatg gtgggctcgc aaatgccgat tgctgtctct
     8701 catggtgaag ggcgcgtgga agtgcgtgat gcggcgcatc tggcggcact ggaaagcaaa
     8761 gggctggtgg cactgcgcta tgtcgataac ttcggcaaag tcactgaaac ctacccggct
     8821 aacccgaacg gttcaccgaa cggtattacg gcggttacga ctgaaagtgg tcgagtcacc
     8881 attatgatgc cgcacccgga acgtgttttc cgtactgtca gcaactcctg gcatccggaa
     8941 aactggggcg aggatggccc atggatgcgc attttccgca atgcgcgtaa gcagttgggg
     9001 taagtcatca gttcattggt tttgcagccc ggatgcggca ttaagcacca accactatca
     9061 taagctccca actccgggag cttttttgtg tctgtaaatc acgacaatgg gtggtttacc
     9121 gtgtcgcttt ctggcgacac ttaaatgatt gattttattg taatctaata agtttatctt
     9181 aagttgtctc tttttagcga cacagtggct gatttacatt caaatagcga agaagccata
     9241 aatccctcaa agcaacgtat aatcagtacg ttaccaaact attttcttta ttggcacagt
     9301 tactgcataa tagtaagcag tggctcattc accgacttat gtcagcccct tcgggacgtg
     9361 ctacataaaa ttcgaatgac gcacaacaag gtgcctgccg tccaacttct gatatcagcg
     9421 tagctatatc aaccatcggg cgaaacgtcg agttaggcac cgccttattc cataacaaag
     9481 ccgggtaatt cccggctttg ttgtatctga acttcccctc ggttagcatc aggctattcg
     9541 cgtctgacga gagtaacacc ttgaaacgct ggcccgtttt tccccgctca ttacgacaac
     9601 tggtaatgct ggcatttttg ctaattctac tacccctgtt ggtgctggca tggcaagcct
     9661 ggcaaagcct gaatgcgctt agcgatcagg cggcgctggt taaccgcact acgcttattg
     9721 atgcccggcg tagtgaagcg atgaccaacg cggcgctgga gatggagcgt agctaccgtc
     9781 agtattgcgt gctggacgac cctacgcttg cgaaggttta tcaaagccag cgcaagcgtt
     9841 acagcgaaat gctcgatgcc cacgcaggcg tactgccaga cgataaactt taccaggcat
     9901 tgcgtcagga tttgaacaat ctggcccaac ttcagtgtaa caacagtggt cccgatgctg
     9961 ccgccgccgc gcgtcttgaa gcctttgcca gtgccaatac cgaaatggtg caggccacgc
    10021 gcacagtggt gttctctcgt gggcagcaac ttcagcgtga aatcgccgaa cgtgggcaat
    10081 attttggttg gcaatcgctg gtgctatttc tggtgagtct ggtgatggtg ctacttttca
    10141 cgcggatgat tatcgggccg gtgaaaaata tcgagcgcat gatcaaccgt ctgggggaag
    10201 ggcgttctct gggcaatagc gtttcgttca gtggaccgag cgagttacgc tcggttgggc
    10261 aacgtattct ttggttaagt gagcgcctgt catggctgga atcccaacgc catcaatttt
    10321 taagacattt atctcatgaa ttaaaaacgc cactggcgag tatgcgcgag ggcactgaat
    10381 tactggctga ccaggttgtc gggccgctta cgccagagca aaaagaggtg gtgagcattc
    10441 ttgatagcag cagccgcaat ttgcaaaaac tgatcgaaca actgcttgat tacaaccgta
    10501 aacaggcgga cagtgcggtg gaactggaga atgttgaatt agcaccgctg gtggagacag
    10561 tggtttctgc tcatagcctg cccgcacggg ctaaaatgat gcataccgac gtcgatctca
    10621 aagcaacagc ttgcctggcg gagccaatgc tgctgatgag cgtactggat aatctttact
    10681 ccaatgcggt gcactacggg gctgaatccg gtaacatttg ccttcgcagc agttcacatg
    10741 gtgcgcgggt ttatattgat gtcatcaata caggcacgcc cattccgcaa gaggaacgcg
    10801 ccatgatctt cgaacccttt tttcagggaa gccaccagcg aaaaggggcg gtgaagggca
    10861 gcggtctggg attaagcatt gccagggatt gtattcgccg tatgcaaggg gaactgtatc
    10921 tggtcgacga gagcgggcaa gacgtttgtt tccgcattga attaccgtcg tcgaaaaaca
    10981 cgaaataatg ctaacagcct atcctatcag gctattttat ttgtcagttc ggccccgggc
    11041 agtgctcgaa atcttcacgt acgatatgta cgctccggtt tctccgcgct gtccatgtcc
    11101 agactgtctg caacaattac gcccactggg ataggcttta agtctggtga atatgcgaca
    11161 catttttcaa cgattattgc cccgacggct gtggctggca gggctgccat gtctggcatt
    11221 gctgggttgc gtgcagaatc acaataagcc agccattgat acgccagcag aagaaaaaat
    11281 tccggtttat caactggctg actatctttc tactgaatgt agcgacatct gggcgctgca
    11341 aggtaaatca accgaaacca atccgcttta ctggctgcgg gcgatggatt gtgctgatcg
    11401 tttaatgcct gcgcagtcgc gtcagcaagc tcgccaatat gacgacggca gctggcaaaa
    11461 tacctttaaa caagggattt tgctcgccga cgccaaaatt acgccgtatg agcgccgcca
    11521 gctggtcgcg cgtattgaag cgttaagtac cgagatcccg gcacaggttc gcccactgta
    11581 tcaactctgg cgcgacggtc aggcgctgca actgcaactg gcggaagagc gccaacgtta
    11641 cagtaaactc cagcagtcca gcgacagcga gctggataca ttgcgccagc aacatcacgt
    11701 tctacagcag caactggaac tcaccacccg caagctggaa aacctgaccg atattgaacg
    11761 ccagctctcg acccgcaaac cgactggaaa tttctcgccg gatacgccgc acgagagtga
    11821 aaagcccgcg ccatccaccc atgaggtcac tcctgatgag ccataaacct gcgcatttat
    11881 tattggtcga tgacgatccg ggattgctga aactgcttgg cctgcgcctg accagcgaag
    11941 gctacagtgt ggtcacggcg gaaagtggcg ctgaaggatt acgggtactg aatcgcgaaa
    12001 aagtagattt agttatcagc gacctgcgga tggatgaaat ggacggtatg cagctatttg
    12061 ctgaaatcca gaaagtgcag ccgggaatgc cagtaattat tcttactgcg catggttcta
    12121 tccccgatgc cgttgctgca acacagcagg gcgtttttag tttcctcacc aagcctgtcg
    12181 ataaagatgc gctatatcag gcaattgacg atgcgctgga gcaatccgcg ccagccaccg
    12241 atgaacgctg gcgcgaggca attgtcaccc gcagcccgct gatgctgcgt ttgctggaac
    12301 aggcgcggct ggtggcgcaa tcagacgtca gcgttttgat taacggtcag agcggcaccg
    12361 ggaaagagat tttcgcccag gctatccaca acgccagccc gcgcaacagc aaaccattta
    12421 ttgctattaa ctgtggcgca ttgcccgagc aattgctgga gtcggagctg tttggtcatg
    12481 cgcgtggcgc gtttactggc gctgtcagca atcgcgaagg tttattccag gcggcggaag
    12541 gcggaacgct atttctcgat gaaattggcg atatgcctgc gccgttacag gtcaaactgc
    12601 tgcgcgtgtt gcaggagcgt aaagtgcgcc cgctgggcag taaccgcgat attgatatca
    12661 atgtgcggat tatttctgcc actcaccgtg atctgccaaa agcgatggcg cgcggggaat
    12721 tccgcgaaga tctctattac cgcctcaacg ttgtcagcct gaaaattccg gcactggcgg
    12781 agcgcacaga agacattccg ctactggcaa atcacctgtt gcgccaggcg gcagagcgac
    12841 ataaaccgtt tgtccgcgcg ttctctaccg atgcgatgaa acgcctgatg accgcgagct
    12901 ggccgggtaa tgtgcgccag ttggtcaacg tgattgaaca gtgcgtggcg ctgacctcat
    12961 ctccggtgat tagtgatgcg ctggtggagc aggcgctgga gggtgaaaat acggcgctgc
    13021 caacctttgt tgaggcacgt aatcagtttg aactcaacta tttgcgtaag ctgctgcaaa
    13081 tcaccaaagg caacgtcacc cacgcggcga gaatggcggg gcgcaaccgg acagaatttt
    13141 ataaactgct ttcccgacac gagctggatg caaacgattt caaggaatga attggcgtta
    13201 tgtgttacgt ttagcagatc aaaagacagg cgaccttttc aaggaatagc atgaaaaaga
    13261 ttgatgcgat tataaaaccc ttcaagctgg acgatgtccg cgaagcactg gccgaagtcg
    13321 gtattaccgg catgacggtg accgaagtga aaggctttgg tcgccagaaa ggccataccg
    13381 agctgtaccg cggcgcggag tatatggtgg attttctgcc gaaagtgaaa attgagattg
    13441 tcgtaccgga cgacattgtc gatacctgtg tcgataccat tattcgcacg gcgcaaaccg
    13501 gcaaaatcgg tgacggtaaa atcttcgtct ttgacgtggc acgggtcatt cgcatccgta
    13561 ccggtgaaga ggacgacgcg gcaatttaaa ccgcgtcaag ggttgccgga tgtttccatc
    13621 cggcaacatc aaattacagc accttatgcg ggccaaagca ttcgtaatga atgttttcct
    13681 gcttcacgcc cagatccact aactgtttcg cggtaaactg catgaagcca accgggccgc
    13741 agagatagaa ctgcattgtc ggatcgctga acgcaccttc cagtttgctc aaatccatca
    13801 gaccttcgct atcaaactga cctttagcgc gatcggcttc gctcggctga cgatgccagg
    13861 tgtgcgcggt aaagcgcggc agtgactgcc ccagttcctt aacttcatcg gcaaaggcgt
    13921 gaacatcgcc attttctgcc gcatggaacc agttcacttg tgctgtgtgg cctgcttttg
    13981 ccagcgtgtc gagcattgcc agcattggcg tttgaccaac accggcagag attaacgtca
    14041 ctggtgtgtc atctgcgaca gccataaaga aatcacctgc cggagcgacc agtttcacga
    14101 catcgccaac attggcgtga ttgtgcaacc agttggatac ctgcccaccc tcttcgcgtt
    14161 tcaccgcaat acgatagcct ttgccatccg gtttgcgagt caaagagtac tgacgaattt
    14221 cctgatgcgg gaaaccttcc ggcttcagcc agacgccgag atattgcccc ggacggtatt
    14281 ctgccactgc gccaccgtcg accggctcca gttcgaagct ggtgataagc gcgctgcgcg
    14341 gtgttttagc cacaatgcgg aaatcgcgag taccttccca accaccggct ttgctggcgt
    14401 tttcgttata gatttccgcc tcgcgattga taaatacatt agccagtaca ccataggctt
    14461 taccccacgc gtccagcact tcctgccccg ggctgaacat ttcgtccagc gttgccaaca
    14521 ggtgttcacc gacgatgttg tactgttccg gtttgatctg gaagctggtg tgcttctgcg
    14581 cgattttttc taccgctggc agcagcgcag gcaggttttc aatattactg gcgtaggcgg
    14641 caatagcgtt aaacagggct tcacgttgat cgccattacg ctggttactc atgttaaaaa
    14701 tttctttgag ttctgggtta tgagtaaaca tacggtcgta gaaatgggcg gttaactttg
    14761 gccccgtttc caccagtaaa gggatggtgg cttttactgt agcgatggtt tgagcgtcaa
    14821 gcatatggtc ttcctttttt tgcatcttaa ttgatgtatc tcaaatgcat cttataaaaa
    14881 atagccctgc aatgtaaatg gttctttggt gtttttcaga aagaatgtga tgaagtgaaa
    14941 aatttgcatc acaaacctga aaagaaatcc gtttccggtt gcaagctctt tattctccaa
    15001 agccttgcgt agcctgaagg taatcgtttg cgtaaattcc tttgtcaaga cctgttatcg
    15061 cacaatgatt cggttatact gttcgccgtt gtccaacagg accgcctata aaggccaaaa
    15121 attttattgt tagctgagtc aggagatgcg gatgttaaag cgtgaaatga acattgccga
    15181 ttatgatgcc gaactgtggc aggctatgga gcaggaaaaa gtacgtcagg aagagcacat
    15241 cgaactgatc gcctccgaaa actacaccag cccgcgcgta atgcaggcgc agggttctca
    15301 gctgaccaac aaatatgctg aaggttatcc gggcaaacgc tactacggcg gttgcgagta
    15361 tgttgatatc gttgaacaac tggcgatcga tcgtgcgaaa gaactgttcg gcgctgacta
    15421 cgctaacgtc cagccgcact ccggctccca ggctaacttt gcggtctaca ccgcgctgct
    15481 ggaaccaggt gataccgttc tgggtatgaa cctggcgcat ggcggtcacc tgactcacgg
    15541 ttctccggtt aacttctccg gtaaactgta caacatcgtt ccttacggta tcgatgctac
    15601 cggtcatatc gactacgccg atctggaaaa acaagccaaa gaacacaagc cgaaaatgat
    15661 tatcggtggt ttctctgcat attccggcgt ggttgactgg gcgaaaatgc gtgaaatcgc
    15721 tgacagcatc ggtgcttacc tgttcgttga tatggcgcac gttgcgggcc tggttgctgc
    15781 tggcgtctac ccgaacccgg ttcctcatgc tcacgttgtt actaccacca ctcacaaaac
    15841 cctggcgggt ccgcgcggcg gcctgatcct ggcgaaaggt ggtagcgaag agctgtacaa
    15901 aaaactgaac tctgccgttt tccctggtgg tcagggcggt ccgttgatgc acgtaatcgc
    15961 cggtaaagcg gttgctctga aagaagcgat ggagcctgag ttcaaaactt accagcagca
    16021 ggtcgcgaaa aacgctaaag cgatggtaga agtgttcctt gagcgcggct acaaagtggt
    16081 ttccggcggc actgataacc acctgttcct ggttgatctg gttgataaaa acctgaccgg
    16141 taaagaagca gacgccgctc tgggccgtgc taacatcacc gtcaacaaaa acagcgtacc
    16201 gaacgatccg aagagcccgt ttgtgacctc cggtattcgc gtgggtactc cggcgattac
    16261 gcgtcgcggc ttcaaagaag cagaagcgaa agaactggct ggctggatgt gtgacgtgct
    16321 ggacagcatc aatgatgaag ccgttatcga gcgcatcaaa ggtaaagttc tcgacatctg
    16381 cgcacgtttc ccggtttacg cataagcgaa acggtgattt gctgtcaatg tgctcgttgt
    16441 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aactttgcaa attcaatata
    16501 ttgcaatatc cgtgtaggcc tgataagcgt agcgcatcag gcaatcttag gtatgtgatc
    16561 atcaaggctt ccttcgggaa gcctttctac gttattgcgc catcaaatct gtcgtaactg
    16621 cgcctcaaca tacaaatagc caatccccaa cacctgttgt gcgcggctta attgcccaaa
    16681 gccaatttgc gttgctttca cgctcggccc ttcgtcgcgg tcgaacgggt taaatcccgt
    16741 ctggcgaata atggtattaa gccagtgctc accaaaggca caactgcgac cgtacaacca
    16801 aatctgatta atattgagga tgttgaggaa gttatacaga ctcaaaccaa tggcattggc
    16861 actgcgatca acccaactgg tgatccacgg ttctccactc taccaggcag cgattaactg
    16921 cgctgtagtc agtttttccg gatcaagctg agtattaacc ggttgtgatt ttagccatac
    16981 ccgcgcctgt ttttttaatg cacttaacga ggcgacggtt tccaggcagc cgtaacgtcc
    17041 acagtcgcag acgacgccat ccggattaac gatggtgtga ccaatctgtc cgctaccata
    17101 caaactgccg cgataaattt gctcgttaat cacgaacgac gagccaatgc cgtaatcaac
    17161 gttgatcacg cagaaatccc gtttctgcga attattttgc catttctccg ccagcgccag
    17221 catcacgcag tcattatcga ccatcacccg aatgccgagc ttctcttcca gcagatactt
    17281 cacctcaacc ggcgttgtcc acggcgcttg cggcatggtt tgcgacacgc cggtcaccgg
    17341 atcaacctga ccgtggattg ccagcgccag attgatggtg cggtccggcc acaatttacg
    17401 gtggcgatgc cagcattttt cgatttcgga cagcagcgcc tgcggcgtcg gtgcatcaat
    17461 ctgtaaatac tcaaattcac ctttcggact taaacaagca ttagcaacct gacactcaat
    17521 actggtgggc gtcacgttca ggcacagcgt ccagtcacct tccggcgcaa tcagccatgt
    17581 accgctacta tgcccgcgcg tctggctttc atcgtcgata ttcaccaccc gtttttcgct
    17641 ttccagttcc tgcaaaatat tactgactgc cggaatcgaa atttgcgcca gccgggccag
    17701 cgttgattta ttggcgcgct tttgccggta cagcagttcc agaatcacgc atttgttatg
    17761 gtggcgaatc tgttgattgt taatgcaggc tctcatcttc actaaacctt gtttagttaa
    17821 ttgcgtgatt attccgcgaa aacattgcca gattgttcaa tacactgcca caaatctttt
    17881 aattcagtat gtcttgttaa tattgagggc accatgactc cagtaaaagt gtggcaagag
    17941 cgcgttgaga tcccgaccta tgaaaccggg ccgcaggata tacatcccat gttcctggaa
    18001 aatcgcgttt atcagggatc gtccggcgcg gtttatccct acggcgtgac cgatacgctg
    18061 agcgagcaga aaaccctgaa atcctggcag gcggtgtggc tggaaaacga ctacatcaaa
    18121 gtgatgatcc tgccggaact gggcggtcgg gtgcatcgcg cgtgggataa agtgaaacag
    18181 cgcgattttg tttatcacaa tgaagtcatt aaacctgcgc tggtggggct gctggggccg
    18241 tggatttccg gtgggattga gtttaactgg ccgcaacatc atcgcccgac cacctttatg
    18301 cccgttgatt tcaccctcga agcccatgaa gacggtgcac agacggtgtg ggtaggcgaa
    18361 acggagccga tgcatggttt acaggtgatg acaggtttca ccctgcgccc tgggcggcgc
    18421 tggaaatcgc cagccgcgtc tataacggca acgccacgcc gcgtcatttc ttgtggtggg
    18481 ccaacccggc agtgaaaggg ggtgaagggc atcagagcgt tttcccaccg gatttgactg
    18541 cggtgtttga tcacggcaaa cgggccgtct ccgctttccc catcgctacc ggcacttact
    18601 acaaagtgga ctactccgct ggagtggaca tttctcgcta taaaaatgtg cccgttccaa
    18661 cctcatatat ggccgaaaaa tcgcagtacg attttgtcgg cgcgtggtgt cacgatgaag
    18721 atggcggttt gctgcacgtt gccaaccacc atattgcgcc aggtaaaaaa cagtggagtt
    18781 ggggacacag tgaatttggc caggcgtggg ataagagtct gaccgacaat aacggcccgt
    18841 atatcgaact gatgaccggt atttttgccg ataaccagcc cgattttacc tggcttgatg
    18901 cttacgaaga gaagcgtttt gagcagtatt tcctgcctta tcattctctg ggcatggtgc
    18961 aaaatgcctc ccgcgatgcg gtgataaaac tccagcgtag tgagcagggg attgagtggg
    19021 ggctgtatgc catctctccg ttgaacggat accgcctggc gatccacgaa atcggcaaat
    19081 gcaacgcgtt acttgatgat gccgtggcac tgatgcctgc gaccgccatc cagggcgtgt
    19141 tgcacggtat caatcctgat aggctgacca ttgagctttc cgatgccgac ggcaatattg
    19201 tactgagtta tcaggaacat cagccgcaag cgttgccgtt accggacgtc gccaaagcgc
    19261 cactggcagc acaagacatt accagtacag atgaagcctg gtttatcggt cagcatctgg
    19321 agcaatatca tcacgccagc cgttcaccgt tcgattacta cctgcgcggc gtggcgctgg
    19381 acccgctgga ttatcgctgt aacctggcgc tggcgatgct ggaatataac cgcgcagatt
    19441 tcccgcaagc ggtggcgtat gccagtcagg cgctgaaacg cgcacatgcg ctgaacaaaa
    19501 acccgcagtg tggacaagca agtttgattc gcgccagtgc ttacgaacgt cagggacaat
    19561 atcaacaagc cgaagaggat ttctggcgtg cggtctggag cggcaacagc aaagccggtg
    19621 gctattatgg cctggcacga ctggcggcgc gtaatggtaa cttcgacgct ggtctggatt
    19681 tttgccaaca aagtcttcgc gcctgcccaa ccaatcagga agtgctttgc ctgcataatc
    19741 tgctgctggt gttaagtggt cgtcaggaca acgcgcgttt gcagcgcgcg aaactgctgc
    19801 gtgattatcc gctgaacgcc attctgtggt ggctgaactg gttcgatggt cgtagcgaat
    19861 cagccctcgc gcagtggcgc ggtctgtgtc agggacgcga cgttaacgcc ctgatgaccg
    19921 ccgggcaact gattaactgg ggaatgccca ctctggcgac agagatgctg aacgcactgg
    19981 actgtcagcg cacgttatcg ctttatctac aagccagctt gctgccgaaa gccgaacgcg
    20041 gcgaactggt cgcaaaagcc attgatgtct tcccgcagtt tgtccgtttc ccgaatacgc
    20101 tggaagaagt ggcggcgctg gagagtattg aagagtgctg gtttgcccgt catttactgg
    20161 cctgcttcta ctacaacaag cgcagctaca acaaagccat tactttatgg caacgttgcg
    20221 tagagatgtc gccggagttt gccgatggct ggcgcgggtt ggcgatccat gcgtggaata
    20281 agcaacacga ttatgaactg gccgcgcgtt atcttgataa tgcttatcag cttgcgccgc
    20341 aggatgcacg tctgcttttc gaacgggatt tgctggataa gttaagtgga gccacaccgg
    20401 agaaacgact ggcgcgtctg gaaaataatc tggaaattgc gctgaaacgc gacgacatga
    20461 ccgcagaact gctcaatttg tggcatctca cgggtcaggc agacaaagcg gcggacattc
    20521 tcgccacgcg caaattccac ccgtgggaag gcggggaagg gaaggtcacc agtcagttta
    20581 tcctcaacca gttattacgc gcctggcagc atcttgatgc cagacagccg cagcaggcct
    20641 gcgaactgct tcatgccgcg ctgcattatc cggagaattt aagcgaaggc cgtttaccgg
    20701 ggcaaactga taacgacatc tggttctggc aggcgatatg cgccaacgcg cagggcgatg
    20761 aaactgaagc gacgcgttgt ttacgtctgg cggcgaccgg cgatcgcacc attaacatcc
    20821 acagttatta caacgatcag ccggttgatt atctcttctg gcaaggaatg gcgctgcgac
    20881 tgctgggcga acaacaaatc gcacagcaac tgtttagtga aatgaaacag tgggcgcaag
    20941 agatggcgaa aaccagtatt gaggcggatt tctttgctgt ttcacaacct gacctgttgt
    21001 cgctgtatgg cgatttacaa cagcagcata aagaaaaatg cctgatggtg gcgatgctgg
    21061 cgtccgcggg actcggggag gttgcgcaat atgaatctgc tcgcgctgaa ttgacggcga
    21121 ttaatccggc ctggccgaaa gcggcattat tcaccaccgt gatgcctttt atttttaact
    21181 acgttcacta aaccctatta actgtaatat ttgagcggca cgcgctgccg ctcatcacaa
    21241 aataattaga ataagccgtt gttaataaaa actctacagg aggcactatg cctaaaaaaa
    21301 tgagaacaac ccgtaatcta ttattaatgg ctacgctatt aggtagcgca ctatttgcca
    21361 gggctgcgga taaagaaatg accattggtg caatatacct tgataccctg ggatattacg
    21421 ctggagtgcg tcagggcgtt caggatgcgg caaaagattc ttcagtacag gtacagttaa
    21481 ttgaaactaa cgcccagggt gatatttcga aagaaagtac ctttgttgat accctcgtgg
    21541 cgcgtaatgt cgatgccatt attttatcgg cagtgtctga aaatggcagt agccgtaccg
    21601 ttcgtcgcgc cagtgaagcg ggtattccgg tgatttgcta caacacctgt attaatcaaa
    21661 agggtgtcga taaatatgtc tcggcgtatc tggtcggcga tccactggaa tttggtaaaa
    21721 aactgggtaa cgctgccgcc gattatttta ttgccaataa aattgaccag ccgaaaattg
    21781 ccgtcatcaa ttgcgaagcc tttgaagttt gtgtgcagcg acgtaaagga tttgaagaag
    21841 tattaaaatc ccgcgttccc ggcgcgcaaa ttgtcgctaa tcaggaaggg actgttttaa
    21901 ataaagcgat ttccgttggt gaaaaactga ttatctccac gccggatctc aacgccatta
    21961 tgggggagtc gggcggtgcg acactcggcg cggtaaaagc ggtacgtaat caaaatcagg
    22021 ccggaaaaat tgctgttttc ggttcggata tgacaaccga aattgctcag gagctggaaa
    22081 acaatcaggt gctgaaagcg gtagtggata tttccggtaa gaaaatgggc aatgctgttt
    22141 tcgcgcaaac attgaaggtt atcaataaac aagccgacgg tgaaaaagtg attcaggtgc
    22201 caatcgatct ctataccaaa acggaagatg gtaaacagtg gctggcaacg cacgttgatg
    22261 gtctgcccta atcgctgatt cgcagaggtc cctatgttca cggcaacaga ggcagtcccg
    22321 gtagcaaaag tggtggcagg aaataagcgt taccccggcg tcgttgcgct ggataacgtt
    22381 aacttcacgc tcaataaagg cgaagttcgt gcgctgttag gtaaaaacgg cgcgggcaaa
    22441 tcgactctca ttcgaatgct taccggcagc gaacgcccgg atagcggtga tatctggatt
    22501 ggcgagacgc gactggaagg tgacgaagct acgttgactc gccgtgccgc tgaactgggg
    22561 gttcgtgcgg tttatcagga attaagtctg gtggaagggc tgacggtggc agaaaacctc
    22621 tgccttggtc aatggcctcg ccgcaacggc atgattgatt acctgcaaat ggcgcaggat
    22681 gcccaacgtt gcttacaggc gctgggcgtt gacgttagtc ctgaacaact tgtttcaacg
    22741 ctaagcccgg cgcaaaagca actggtggaa attgcacggg tgatgaaggg cgagccgcgc
    22801 gtggtcattc ttgatgaacc caccagctcg cttgccagtg cagaagttga actggtgatc
    22861 agtgcggtga aaaagatgtc ggcactgggc gtggcggtga tttatgtcag ccaccggatg
    22921 gaagaaatcc gccgcattgc ctcctgtgcc accgttatgc gcgatggtca ggtggcgggc
    22981 gatgtgatgc tcgaaaacac ctccacgcat catattgtgt cgctaatgct agggcgtgat
    23041 cacgttgata ttgcgccggt tgcccctcag gaaattgtgg atcaggccgt actggaagtc
    23101 cgtgcgttac gccataagcc caagctggag gatatcagct ttacgttacg tcgcggcgaa
    23161 gtgcttggca ttgctggcct gctgggggca gggcgcagtg aattgctgaa ggcgattgtt
    23221 gggctggaga cgtatgaaca gggcgaaatt gttatcaacg gcgagaaaat cacgcgcccc
    23281 gattacggcg acatgctgaa acgcggcatt ggctatacgc cagaaaaccg caaagaagcg
    23341 gggatcattc cctggttggg cgttgacgaa aatacagtgc tgaccaatcg gcaaaaaatc
    23401 agcgccaacg gagtgctgca atggtccacc atccgccgcc tgaccgaaga ggtgatgcag
    23461 cggatgacgg tcaaggccgc tagtagcgaa acgcccatcg gcacgctttc tggtggcaat
    23521 cagcaaaaag tggtgatcgg tcgttgggtc tatgccgcca gccagatttt gttgctcgac
    23581 gagccaacgc gcggcgtcga tatcgaagcc aaacagcaga tttaccgtat tgtccgtgag
    23641 ctggctgccg aaggaaaaag cgtggtgttt atctccagtg aagtggagga gttgccgctg
    23701 gtgtgtgacc gcatcctgtt attacagcac ggcacgttct cgcaggagtt tcactcgccg
    23761 gtcaatgtgg atgagctgat gtccgccatt ctgtctgtgc actgatgaat tttaccgagg
    23821 aacctgaaga tgtctgcttc gtcattacca ttgccgcagg gcaagagcgt ctcgctcaaa
    23881 caatttgtca gtcgccatat taatgagatc ggtttgctgg tggtgattgc cattctttat
    23941 ctggtcttct ccctgaacgc gcctggcttt atctcattga ataaccagat gaacgtgctg
    24001 cgcgatgccg ccaccattgg gattgccgcc tgggcgatga cgctgattat tatctccggt
    24061 gaaattgatg tcagcgttgg gccgatggtg gcttttgtct cggtgtgcct ggcatttttg
    24121 ctgcaatttg aagttccgtt ggcgattgct tgtctgctgg tgttgctgtt aggcgcgctg
    24181 atgggaacgc tcgccggggt gctgcgcggc gtgtttaacg taccaagttt cgttgccaca
    24241 ctggggctgt ggagcgccct gcgcggaatg gggctgttta tgaccaacgc actgccagtg
    24301 ccgattgacg aaaacgaggt gctggactgg ctgggcggac aatttctcgg tgtgccagta
    24361 tccgcgctga tcatgatggt gttgtttgcg ctgtttgtgt tcattagccg aaaaaccgcc
    24421 ttcgggcgct cggtttttgc cgttggcggt aatgccacgg cggcgcagtt gtgcggtatt
    24481 aacgttcgtc gggtgcgcat tcttatcttt accctttcgg gattattagc ggcggtgacc
    24541 ggcattttgc tggcggcacg cctcggttcc ggtaacgcag gtgccgcaaa cggtctggag
    24601 tttgacgtca tcgccgcggt cgtggtcggc ggtacggcac tttccggcgg tcgcggttcc
    24661 ctgttcggta cattgcttgg tgtgctggtg attacgctaa tcggtaacgg tctggtgctg
    24721 ctcggtatta actccttttt ccagcaggtg gtacgcggcg tcatcatcgt ggtggcggtg
    24781 ctggcgaata tcttgctgac ccagcgaagc agtaaagcga aacgctaaac cttaacgccc
    24841 ctggcgcggg tgcggcaggg atgaaaatta atcgtattgt taatgaggga tttatgaaaa
    24901 cgatgctggc agcttattta ccaggaaatt caaccgtcga tctgcgggaa gttgcggtgc
    24961 cgacgccggg gattaaccag gtactgatca aaatgaaatc ctccgggatt tgcggaagcg
    25021 atgtccacta tatctatcat caacactgtg ccacggcggc ggcacccgat aaaccgttat
    25081 accagggatt tatcaacggt catgaaccgt gcgggcagat tgtggcgatg gggcaaggct
    25141 gccgccattt taaagagggc gaccgcgtgc tggtgtatca catttctggc tgtggttttt
    25201 gcccgaactg ccgccgcggc tttcctatct cttgtactgg caaaggaaaa gcggcttacg
    25261 gctggcagcg tgacggcggt catgccgaat atctgctggc ggaagaaaaa gatctgatcc
    25321 tcctgccgga tgcgctgagc tacgaagatg gtgcgtttat cagttgcggc gttggcacgg
    25381 cctatgaagg gattttgcgc ggcgaagttt ccggcagcga taacgtgctg gtggtcggtc
    25441 tggggccggt cggcatgatg gcgatgatgc tggcgaaagg tcgcggtgca aaacggatca
    25501 tcggcgttga tatgctgccg gaacgtctgg cgatggcaaa acagttaggg gtgatggatc
    25561 acggctattt agcaaccacc gaaggtctgc cgcagattat cgccgaactc acccacggtg
    25621 gcgcggatgt tgcgctcgat tgttctggta atgccgcagg tcgcttgctg gcactgcaat
    25681 ccaccgctga ctggggacgg gtggtttaca ttggtgaaac cggaaaagtg gaattcgagg
    25741 tcagcgccga tctgatgcac catcaacggc ggattatcgg ctcctgggtg accagtctgt
    25801 tccatatgga aaaatgcgcc cacgatttga ctgactggaa gctgtggccg cgtaacgcca
    25861 ttacccatcg cttctcgctg gaacaggcag gtgatgccta tgcgctgatg gcgagcggca
    25921 aatgcgggaa agttgtgatt aacttcccgg attaaggagt tagcggatga ccatctatac
    25981 cttatcccac gggtcgctaa agctggacgt ttctgaccag ggcggcgtaa tcgaaggctt
    26041 ctggcgcgat acgacgccgc tcttacgtcc tggtaaaaaa agcggtgtgg caaccgatgc
    26101 ctcatgtttt ccgctggtgc catttgcaaa ccgggtgagc ggcaaccgtt ttgtctggca
    26161 ggggcgtgag tatcaactgc aaccgaatgt tgagtgggat gcgcactatc tacacggcga
    26221 tggctggctg ggcgagtggc aatgtgtctc gcacagcgat gatagtttgt gtctggtgta
    26281 tgaacatcgc agcggtgtct atcactatcg ggtaagtcag gcgtttcatt taacggcgga
    26341 tacgctgacg gtgacgctct ctgtcaccaa tcaaggggca gagacgctgc catttggtac
    26401 cggctggcat ccttattttc cgttgtcacc acaaacgcgg attcaggcgc aggcgagcgg
    26461 ttactggctg gaacgggagc agtggctggc gggtgagttt tgcgagcagc taccgcagga
    26521 actggatttt aaccagctcg cgccgttgcc gcgccagtgg gtgaacaatg gttttgccgg
    26581 atggaatggt caggcccgca tcgagcagcc gcaggaaggt tatgccatca tcatggaaac
    26641 gacgccacct gcaccgtgtt atttcatctt tgtttccgac cctgcgtttg ataaaggata
    26701 tgcgtttgat ttcttctgtc tggaaccgat gagccatgcg ccggatgatc atcatcgccc
    26761 ggaagggggt gatctcattg cgctagcacc aggggaatca acaacttcag agatgtcgtt
    26821 gcgggtagag tggctgtaat ttattaaatg ccggataagg cgttcacgcc gcatccggca
    26881 tctttcctaa cgccgatcga gagaaattgc ccccggcccg gtaattgcca atagcaagaa
    26941 cgcgccagcg atactgacat tcttccagaa attaatcata tttggcccaa ccgcatcgcc
    27001 ggtcatatcc cagtaatggt gaccaatcac cgccgtaccc agcgtgtaga aaataaacag
    27061 caccgccagc ggacgggtga aaaagccaag cacgattaat atcgcggcgg gcacttccat
    27121 aactaccgca ataatcgctg ccagcatcgg cattggcgcg cccaacgagg ccatatattg
    27181 gaccgtaccg tcaaagccca tcattttggg aaaaccaaaa ataatgaaaa ttaagaccac
    27241 ggcgatacgg gcaattaata acaaaacggg gcgggcagct ccaaaatcaa aataacg
//
LOCUS       DAZPJP010000039        27109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPJP010000039 DAZPJP010000000
VERSION     DAZPJP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.039"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..925
                     /locus_tag="ACHU9J_001512"
     CDS             <1..925
                     /locus_tag="ACHU9J_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1905092.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHU9J_001513"
     CDS             complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHU9J_001513"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1905093.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHU9J_001514"
     CDS             1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHU9J_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1905094.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3886..4281
                     /locus_tag="ACHU9J_001515"
     CDS             3886..4281
                     /locus_tag="ACHU9J_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1905095.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4301..4891
                     /gene="diaA"
                     /locus_tag="ACHU9J_001516"
     CDS             4301..4891
                     /gene="diaA"
                     /locus_tag="ACHU9J_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1905096.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4901..5476
                     /gene="dolP"
                     /locus_tag="ACHU9J_001517"
     CDS             4901..5476
                     /gene="dolP"
                     /locus_tag="ACHU9J_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1905097.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5590..6630)
                     /locus_tag="ACHU9J_001518"
     CDS             complement(5590..6630)
                     /locus_tag="ACHU9J_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1905098.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6703..7338)
                     /locus_tag="ACHU9J_001519"
     CDS             complement(6703..7338)
                     /locus_tag="ACHU9J_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1905099.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHU9J_001520"
     CDS             7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHU9J_001520"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1905100.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7964..8407)
                     /locus_tag="ACHU9J_001521"
     CDS             complement(7964..8407)
                     /locus_tag="ACHU9J_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1905101.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHU9J_001522"
     CDS             8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHU9J_001522"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1905102.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8747..9250)
                     /locus_tag="ACHU9J_001523"
     CDS             complement(8747..9250)
                     /locus_tag="ACHU9J_001523"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1905103.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9244..9768)
                     /locus_tag="ACHU9J_001524"
     CDS             complement(9244..9768)
                     /locus_tag="ACHU9J_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1905104.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9977..10972
                     /locus_tag="ACHU9J_001525"
     CDS             9977..10972
                     /locus_tag="ACHU9J_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1905105.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10981..11859
                     /locus_tag="ACHU9J_001526"
     CDS             10981..11859
                     /locus_tag="ACHU9J_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1905106.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12065..13072
                     /locus_tag="ACHU9J_001527"
     CDS             12065..13072
                     /locus_tag="ACHU9J_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1905107.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHU9J_001528"
     CDS             complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHU9J_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1905108.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHU9J_001529"
     CDS             complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHU9J_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1905109.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAHPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHU9J_001530"
     CDS             complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHU9J_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1905110.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHU9J_001531"
     CDS             complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHU9J_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1905111.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHU9J_001532"
     CDS             complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHU9J_001532"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1905112.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHU9J_001533"
     CDS             complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHU9J_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1905113.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHU9J_001534"
     CDS             complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHU9J_001534"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1905114.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHU9J_001535"
     CDS             complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHU9J_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1905115.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHU9J_001536"
     CDS             complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHU9J_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1905116.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHU9J_001537"
     CDS             complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHU9J_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1905117.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHU9J_001538"
     CDS             complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHU9J_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1905118.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26830..26906)
                     /locus_tag="ACHU9J_001539"
     tRNA            complement(26830..26906)
                     /locus_tag="ACHU9J_001539"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26870..26872),aa:Met,seq:cat)
BASE COUNT         6425 a         7517 c         6809 g         6358 t
ORIGIN      
        1 tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct ctgcgggtaa
       61 ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac atcagacagg
      121 atattacaaa ctcaatgaca gcctggatat taaaaccaca ttacaggcaa acgacattcc
      181 aggactcaca accgaccagg ttgtctctgt taacacccga ttcacacaga taaaaagcag
      241 caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt ctcgttatgg
      301 acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc catttctcgg
      361 ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg tcgatggaat
      421 gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgattc agggaaatct
      481 caccgcccca cagtcgtgca aaattaatca gggcgatgtt attaaggtta attttggatt
      541 catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta ctccagtaga
      601 ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt tgcaaatgcg
      661 catcgacggt acaactgggg tagtagacca gtacaacctg gtcgccaggc gaagaagttc
      721 agacaatgtg cccgatgtcg gtattcgtat tgaaaatctc ggcggcggag ttgcaaatat
      781 tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg tcaatatgcg
      841 cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc agggcaccgc
      901 caccattacc gtcatggtgc ggtaaaagaa gacgttgcgt tttgattaac gcaacgcttc
      961 ggcactgtta cccctgctgc tccagcgcat acttatacag cgcatttttc ttcacgccat
     1021 gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt agcagcgcca
     1081 gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc tttatgacct tcgacaatca
     1141 gcaccatttc gcctttgcga cggttttcat cttcctttac ccacgccagc agctcgccaa
     1201 cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc acgcgccaga accacgtagc
     1261 gggattcgcc taataccgca acgatatctt ccaggctatc taacagacgg tgggtagatt
     1321 cataaaaaat cagcgtgcgc ggctccgctt caatggcttt tagcgcatca cggcggcctt
     1381 ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa cccgcggcgc
     1441 ttaacgcagt gatagcagca cacggtccgg gtagcggcac cacacgaatc cccgcttcac
     1501 ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg gcatcggaaa
     1561 ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct ttttgttgtt
     1621 cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc agcaataaac
     1681 cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc tctaacgcac
     1741 gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc tggccctgag
     1801 aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt taatattgag
     1861 cattgcgtaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     1921 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca
     1981 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     2041 tcttcagcag atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat
     2101 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     2161 gcaagaactg aacgattctc agcgtcgcga gaaaacactg ctggcggtag agattaaact
     2221 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt
     2281 agaacaaaac cagcaagcgc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc
     2341 ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggcg cgaaagaaaa
     2401 aaagcagaat attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa
     2461 tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt
     2521 ctggtttgat aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcaaaa
     2581 acgttatccg aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc
     2641 gtttaaacca gcctcgacca acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc
     2701 ggtatttggt cgcaccattc agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc
     2761 agtggcggct caggtagctg ccgcacctgc cgcagacgta gctgaacaac ctcagccgca
     2821 aaccgtggat ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca
     2881 gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc
     2941 acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca
     3001 gatcttaagc caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa
     3061 taacgttgaa gagttgctga agagcaacac cccgctgaac gtgctggcac tgaaccagcc
     3121 agagaatatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcacctg aagacgaagc
     3181 gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc
     3241 acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaactggg
     3301 cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa
     3361 cggcggttct ggtatcgctt taacgggtac cccgattact cccagagcga caaccgactc
     3421 cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa
     3481 taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat
     3541 caaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaatgctgt acgccagctc
     3601 ccgcagcgca caaggcaccg caggcccgga tttccgtctg gagatggaag gtttgcagta
     3661 cagcgaaatc ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc
     3721 ggtgaataac gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct
     3781 ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg
     3841 aagcctgacg gcaaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca
     3901 acaaggtcag gtagtccccg tcagttaacc accaaacaga ccggcgatgc gtgggaagca
     3961 caagcgcgtc gctggctgga aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag
     4021 cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt tgtcgaggta
     4081 cgctatcgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa
     4141 cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact
     4201 gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat
     4261 gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc
     4321 ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat
     4381 ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg
     4441 tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt
     4501 cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac
     4561 ggcgattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg
     4621 tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa
     4681 agcagttgaa gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg
     4741 cggcgagctt gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg
     4801 tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga
     4861 taacacgctt ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat
     4921 cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac
     4981 cgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga
     5041 tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga
     5101 aacccgtatt aatgtcacgg cgtatcaggg caaagtgctg ctggttgggc agtcaccaaa
     5161 cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt
     5221 gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg
     5281 gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt
     5341 gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc
     5401 gaaagcggcg gcagatattg ccagccgggt gagcggcgta aagcgggtaa ccacagcgtt
     5461 tacgtttatt aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca
     5521 ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt
     5581 tccctcaaat cagaataaca gcgccagccc gccgacaatc acgcctgaca ctgccaccat
     5641 cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg
     5701 caaactcact gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc
     5761 cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc
     5821 tgctaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg
     5881 gaataaccag acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt
     5941 actccagaaa agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc
     6001 cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac
     6061 cagcgtcgca atcagcaaca ccatcaccaa cccggccacc agacgaatcg ccgcaaaacc
     6121 ccagccgagg acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat
     6181 ccagaatgcc agcgcaccgc ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg
     6241 agccgcacag caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc
     6301 gcgaaagcgc gattgtccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat
     6361 caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga aatagatcat
     6421 cgcgtaatcc aacgccgcct gccacggatt agcatccgcc tgcgcaagga tagatttacc
     6481 gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc atttcacata
     6541 ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt
     6601 tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt
     6661 gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg
     6721 taattctgaa gagcttaaaa tagtcacgcc ttcatgctca ggccgcatcg cctctgccag
     6781 catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggca gtaaacgaaa
     6841 cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gcaacatcga
     6901 cgggcgagca atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc
     6961 tttgacgcgg ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag
     7021 catatgctgc gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc
     7081 ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa
     7141 cacaatgtcg atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg
     7201 attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac
     7261 tttcggttcg ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt
     7321 aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct
     7381 catgcaccag gcttaataga ccatgaggta attatccccg attgtgtgaa attcgcctca
     7441 tccaatgcaa caacgcggag gaagcatgag taagaaaatt gccgttttaa tcagtgatga
     7501 atttgaggat tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt
     7561 gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt
     7621 gaccatcgat aaatccatcg atgaagtgac gccagcggag tttgatgccc tgctgctacc
     7681 gggtggtcat tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga
     7741 ttttgtgaat agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag
     7801 cgccgatgtg attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa
     7861 aaatgcgggc gcggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac
     7921 cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc
     7981 ctgagtcgcg tagccaaatc atttttttac caaagcccag cgtgttgtca gtgaatttga
     8041 tttcatcgag ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac
     8101 gattgtacgc ctggcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt
     8161 taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa
     8221 ccgcagcctg cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga
     8281 aggcgacttt ctgcgcatca aacagataaa aagcattagc gcaccacagt tccccttcct
     8341 gctgcacaca ccaggtgaca acatgttgtt ttgccaacca acggctgatg gtgatgagtg
     8401 tttccattgc tgctctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg
     8461 acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc
     8521 acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc
     8581 gggaaaggag aactgacgct ggcgttttct gcgccagtgg gcgatcgttc gctggcgtta
     8641 cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa
     8701 ggcgcagggt ttgcagagct gttaagcagt ctgcaaaccc cggagattaa aagcgattga
     8761 aatgctcgtg atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat
     8821 gtacctggaa ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca
     8881 gttcaaagcc gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc
     8941 cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac
     9001 ggtatttttc atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca
     9061 catcaaccgg actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca
     9121 gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac
     9181 tttcgaatga gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc
     9241 gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc
     9301 ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag
     9361 ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc cttcaatcac
     9421 cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag
     9481 atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt
     9541 gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca
     9601 gcggccttca agaaactcca gctcaccatc atccagcgcc tgacggaatt gccagcttaa
     9661 gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa
     9721 cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc
     9781 ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa
     9841 caattccata tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat
     9901 tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgaaagcgca
     9961 accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc
    10021 ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata ctaatgcccg
    10081 tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca
    10141 tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc
    10201 ccgttggcag cgcgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc
    10261 cgacctcgcc atgctggagt acgccgccga gcgttacccg catattgagc gtcatgtgtc
    10321 agtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgatgt
    10381 tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt
    10441 cacacctgtc ccgctggaag tctttgcttt tggcagcctg tgcattatgt cggaaggtcg
    10501 ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc
    10561 ggcccgtttc gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt
    10621 gctgatcgac cgttatcagg atggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg
    10681 ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct
    10741 ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca
    10801 gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg
    10861 taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat
    10921 gtccgaaggc acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca
    10981 atgaaatatt ccttagggcc agtgctgtgg tactggccaa aagaggcgct ggaagaattt
    11041 tatcagcagg ccgccaccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc
    11101 aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagc
    11161 gggaagcaga tagtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa
    11221 ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg
    11281 aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac
    11341 gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag
    11401 ctttcccgcg actggctggt gaatctgctt aatcagtgcg ataagttggg gattcgcaac
    11461 cagtttgaag tggaagtcct gagctacggt catctaccgc tggcctactc cgcccgctgc
    11521 tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat
    11581 ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt
    11641 cagaccatga gcggctacgt ttacaacctc ggtaacgagc tgacatccat gcagggattg
    11701 gttgatgtgg tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc
    11761 cgcgctaatg aaaatggcgc tgcgccactg ccgttgacgg caaacagcga ctgtaacggc
    11821 tactggcggc ggctggctgg gctggagctg caagcctaag taaatatgct cgtcatactt
    11881 caagttgcat gtgctgcatc tgcattcgtt caccccagtc acttacttat gtaagctcct
    11941 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagcac agctcacttt
    12001 gttaacaact ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc
    12061 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca ttcccgaagg
    12121 ttcttcagcg cgagaagcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
    12181 cggctatcat cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc
    12241 cacgtcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
    12301 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa
    12361 tacactctat ccggggcgaa tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg
    12421 gacaatgatg gcgctgcgtc gccatatgag cggcgatatt gataatttcc cccgcgatgt
    12481 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
    12541 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact
    12601 ggcggcgcag cttggtctgc cgttcgcgtt tgcctcacac ttcgcgccgg atatgttgtt
    12661 ccaggcgctg catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata
    12721 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctatt
    12781 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccggac aactgccgcc
    12841 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatggcg tgcagcaggc
    12901 gctgagtatg tcgttggtgg gtgataaagc gaaagtgcgt cacggcttgc agtcgatcct
    12961 gcgcgaaacc gacgccgatg aaattatggt taacgggcag attttcgacc accaggcgcg
    13021 cctgcattcg tttgaactgg cgatggatgt gaaggaagag ttgttgggat agtgtgtctt
    13081 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtagacctga taagcgaagc
    13141 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
    13201 ccggcagcaa attaaagctc gataagatat ggaccagtgc gttgccaacg ccaaacacca
    13261 gaatcagcgt aatcatcggc ttgccgcccc agacacggaa tttcgggctg ccaaagcgtt
    13321 tacgcgatgc gcgggctaac agcgccggaa caattgccgc ccagatggta gctgctaaac
    13381 cagcataacc aatggcgtac aggaatccgt tcgggaacag tagaccaccc acaacaggcg
    13441 gggcaaaggt cagcaatgcc gttttcaagc gccccagggc tgagtcatcg aaaccaaaca
    13501 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
    13561 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
    13621 cctgtaccag cacatcaata ttaccgccct tctctgcaat accgataaac tccggacgcg
    13681 ggatgttacc catcgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
    13741 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggctcg
    13801 gcacgttacc gtgataacca aacgatgcca gacagaacgg cagggtcatc agcaaatacg
    13861 gcgcataaga tgcattgctt tcggcgacgt tgaacaatgt cgcaggctgc acatgcccca
    13921 gtaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagtaca atcgccgtca
    13981 tgcggctgac ggccttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
    14041 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaattg
    14101 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatac
    14161 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
    14221 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    14281 aggtaaagat cagcgccgcc attgaccaga aaaaccacgc cccggacatg accactggca
    14341 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    14401 acggtgacgt ttgggtggtg gttagtgttg ccatgagagc ttctctccag tgaaaaatag
    14461 tgcgactgcg ttgttatgca ttgcactgta ccagtacacg agtacaaaag acagaaaaaa
    14521 agccccgatg gtaaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    14581 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggag
    14641 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacggcca ccttcacggc
    14701 gttcaccgct gaagttacga ccgccttcac gacgttcgcc accgaaacca cgaccaccgc
    14761 cacgacgctc accgccagta tgcgggtgtg catcgcccag taactgcatg ttcatcggct
    14821 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    14881 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc aatgtaacgg ctactgatgt
    14941 cgccttcgtt agcaatcgca ccaacgatat gacgaacttc aacaccatca tcgcggccca
    15001 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    15061 cacggtcacc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    15121 ggaattcacg tttcggacgc atcggcgcat ctggcggtac gatcagagta cgttcaccct
    15181 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    15241 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    15301 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    15361 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    15421 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    15481 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaactacc aggctgatac
    15541 gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat caggatgtcc agacgaccat
    15601 ctttcaggcg ttccagtgtc tgttcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    15661 cgctgttgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    15721 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    15781 ttttgcgcat accccagaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    15841 tggactgaat gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    15901 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    15961 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    16021 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    16081 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    16141 ggccgccgta cagagcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    16201 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    16261 tcagctcagg atcaagattc tgcaacagag gtaaagagaa tgctgcagtt tttccgctcc
    16321 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    16381 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    16441 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    16501 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    16561 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    16621 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    16681 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    16741 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    16801 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    16861 ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc
    16921 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    16981 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    17041 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    17101 cttgtcacga ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt agttgtaagt
    17161 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    17221 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    17281 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    17341 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    17401 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    17461 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    17521 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    17581 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    17641 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    17701 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttctagaac
    17761 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    17821 agcgatttga gagatgtgga ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    17881 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    17941 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcgatctt
    18001 cacagtaccg tcatcttcga tttcgatggt agtgccagtt tcttcggtca gggcacggat
    18061 aacagagccg cctttaccga taacgtcttt gatcttatcc gggttgatct tgatggtatg
    18121 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    18181 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    18241 ggtgatacct tcaattttga tatccatctg cagtgcagag ataccgtcgc gggaacctgc
    18301 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    18361 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgattgg
    18421 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaagaacc
    18481 gttggattcg gtgatttcag acactacacg tacggtgtac gggaatttgt ccatatccgg
    18541 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    18601 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    18661 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cggtacccag
    18721 cgttgcagta accagtgcct gcgtttcacc gcgggtgaac agcgcagaac cgtgagtacg
    18781 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    18841 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatag cgtgcagaat
    18901 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    18961 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    19021 actcagacga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    19081 cggctgccag tcccaacgcg gtttaccggc ttctttcact agttcattga tgttctgaat
    19141 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctgat cttcgctcag
    19201 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    19261 cagtttgctc tctttcagct cgtcctgagt cgggttcagt acgtactggt cattgatgta
    19321 acctacgcgg gcagcaccaa tcgggccatt gaacggaata ccagacagag acagtgctgc
    19381 ggaagcacca atcatcgcga cgatatccgg gttaacctgc gggttaacag aaaccacggt
    19441 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    19501 aatcagacgc gcgatcaggg tttcgccttc gcttggacga ccttcacgac ggaagaagct
    19561 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    19621 gtcctgacct ggtttggctt ttttctgtcc aacaacggta acgaataccg cggtgtcatc
    19681 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    19741 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    19801 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    19861 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    19921 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    19981 cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg
    20041 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    20101 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    20161 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    20221 aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    20281 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    20341 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    20401 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    20461 attcaaccac caggcgacga ggcgcgacgc ggccttcatc gtcaatttcg cccataccga
    20521 taaacttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    20581 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    20641 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    20701 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcaccatcc
    20761 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    20821 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    20881 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    20941 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    21001 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    21061 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    21121 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    21181 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgcctg
    21241 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    21301 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    21361 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    21421 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    21481 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    21541 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    21601 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    21661 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    21721 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    21781 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    21841 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    21901 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    21961 aagcgatggt acgttggatc tcaatgattt cgaatacttc gatcacatcg ccagtgcgga
    22021 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    22081 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    22141 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    22201 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    22261 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    22321 tgacggagta gtaacgcaga tccaagcttt ccgcttcaat cactttacgt gcagaggcat
    22381 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    22441 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    22501 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    22561 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    22621 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    22681 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    22741 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    22801 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    22861 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    22921 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    22981 gcagcaggat agcgtccagc agttcatcga tgccggtacc cgctttcgca gatacgtgta
    23041 cgaactggct ttcaccgccc cactcttccg gcaggatgcc atactgggag agttcgttct
    23101 taacacgatc cggatcggct tctggtttat caatcttgtt cactgcaacc accaccggta
    23161 cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg cggcatcacg ccgtcgtcgg
    23221 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc attgaagtaa
    23281 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    23341 atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggcaactttc gttgaacgaa
    23401 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    23461 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    23521 tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt gcggtttcct
    23581 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    23641 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatggtt tcgccgatca
    23701 caacgtcacg gttaacggcc tgagctggct tctggaagcc ttgctgcagg gaagaacctt
    23761 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    23821 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    23881 gaccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    23941 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    24001 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgatgcg
    24061 cttcttcttc ggctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    24121 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    24181 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcagcctca cgttcagctt
    24241 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctt
    24301 cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa
    24361 aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg gtaccaggaa
    24421 tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct gaatttttct
    24481 gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg tcagcagact
    24541 tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc tgtcgctctg
    24601 cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca gtttattacg
    24661 cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg gccttttcgt
    24721 cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga tcttccagcg
    24781 tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctacccct tcaaggttca
    24841 gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg
    24901 ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca aggccttcga
    24961 tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag aagccttctt
    25021 ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg
    25081 ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt
    25141 cccaaccgct cagctgcgaa gccagacgca cgttctgacc gttacggcca atcgcctgcg
    25201 ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc accacgatag
    25261 aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta tcatcccaca
    25321 ggacgatatc gatacgctcg ccacccagtt cagtagacac cgcctgaaca cgcgcgccac
    25381 gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc accgcgattt
    25441 tcgcacgaga acccggatcg cgagccgctg ctttaatttc aatcacttct tcgccgattt
    25501 ctggcacttc aatacggaac agttcgatca gcatttccgg cttggaacga gtgacgaaca
    25561 gttgcgcgcc acgcgcttcc gggcgaacgg aataaagcac gccacgaacg cggtcgccag
    25621 ggcggaagtt ttcacgcggc agcatatctt cacgcaggat cacggcttca gcgttgttgc
    25681 ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg atgatttcac
    25741 cttcgtgttc acggaactga tcaaccacca tcgcacgttc ggcttcacgc actttctgca
    25801 cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac tcaatctgat
    25861 cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct tcaagggtga
    25921 tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaag gtgtcaaaat
    25981 caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat tttttctttg
    26041 ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt
    26101 cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc ttttcacctc
    26161 atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc agcgcgaaca
    26221 cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct ttgataacgc
    26281 cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc tcttctccga
    26341 caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt gaggagactt
    26401 ccaggttata agcaacggtg atgggatctt caacatccag cacagcactt acctggtggc
    26461 tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag atgcgcagtg
    26521 tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg gcctcaactg
    26581 gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc cccaagacat
    26641 aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc ccgataaatc
    26701 ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact ttccagaagg
    26761 attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa aaaggactct
    26821 aagggaaagt ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac
    26881 gagctaccag gctgctccac cccgcgcctg aaacgtggca aattctactc gttttgggta
    26941 aaaaatgcaa atactgctgg gatttggtgt accgagacgg gacgtaaaat ctgcaggcat
    27001 tatagtgatc cacgccacat tttgtcaacg tttattgcta atcatgtgaa tgaatatcca
    27061 gttcactttc atttgttgaa tacttttgcc ttctcctgct cccccttaa
//
LOCUS       DAZPJP010000040        26578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPJP010000040 DAZPJP010000000
VERSION     DAZPJP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.040"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            72..803
                     /gene="artJ"
                     /locus_tag="ACHU9J_001540"
     CDS             72..803
                     /gene="artJ"
                     /locus_tag="ACHU9J_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1905119.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(978..2105)
                     /gene="rlmC"
                     /locus_tag="ACHU9J_001541"
     CDS             complement(978..2105)
                     /gene="rlmC"
                     /locus_tag="ACHU9J_001541"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1905120.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(2146..2634)
                     /locus_tag="ACHU9J_001542"
     CDS             complement(2146..2634)
                     /locus_tag="ACHU9J_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1905121.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2694..3539)
                     /gene="potI"
                     /locus_tag="ACHU9J_001543"
     CDS             complement(2694..3539)
                     /gene="potI"
                     /locus_tag="ACHU9J_001543"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1905122.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3536..4489)
                     /gene="potH"
                     /locus_tag="ACHU9J_001544"
     CDS             complement(3536..4489)
                     /gene="potH"
                     /locus_tag="ACHU9J_001544"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1905123.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4499..5632)
                     /gene="potG"
                     /locus_tag="ACHU9J_001545"
     CDS             complement(4499..5632)
                     /gene="potG"
                     /locus_tag="ACHU9J_001545"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1905124.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5727..6839)
                     /gene="potF"
                     /locus_tag="ACHU9J_001546"
     CDS             complement(5727..6839)
                     /gene="potF"
                     /locus_tag="ACHU9J_001546"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1905125.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7190..7666)
                     /locus_tag="ACHU9J_001547"
     CDS             complement(7190..7666)
                     /locus_tag="ACHU9J_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1905126.1"
                     /translation="MTSLVVPGLDTLRQWLNDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7754..8656)
                     /gene="rimK"
                     /locus_tag="ACHU9J_001548"
     CDS             complement(7754..8656)
                     /gene="rimK"
                     /locus_tag="ACHU9J_001548"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1905127.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8717..9439)
                     /gene="nfsA"
                     /locus_tag="ACHU9J_001549"
     CDS             complement(8717..9439)
                     /gene="nfsA"
                     /locus_tag="ACHU9J_001549"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1905128.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9423..9710)
                     /locus_tag="ACHU9J_001550"
     CDS             complement(9423..9710)
                     /locus_tag="ACHU9J_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1905129.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9870..10127
                     /gene="grxA"
                     /locus_tag="ACHU9J_001551"
     CDS             9870..10127
                     /gene="grxA"
                     /locus_tag="ACHU9J_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1905130.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10157..10534)
                     /locus_tag="ACHU9J_001552"
     CDS             complement(10157..10534)
                     /locus_tag="ACHU9J_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1905131.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10804..12489
                     /locus_tag="ACHU9J_001553"
     CDS             10804..12489
                     /locus_tag="ACHU9J_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1905132.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12664..13200)
                     /gene="rcdA"
                     /locus_tag="ACHU9J_001554"
     CDS             complement(12664..13200)
                     /gene="rcdA"
                     /locus_tag="ACHU9J_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1905133.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13284..14492
                     /locus_tag="ACHU9J_001555"
     CDS             13284..14492
                     /locus_tag="ACHU9J_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905134.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            14492..15307
                     /gene="ybjI"
                     /locus_tag="ACHU9J_001556"
     CDS             14492..15307
                     /gene="ybjI"
                     /locus_tag="ACHU9J_001556"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1905135.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15393..15677
                     /gene="ybjH"
                     /locus_tag="ACHU9J_001557"
     CDS             15393..15677
                     /gene="ybjH"
                     /locus_tag="ACHU9J_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1905136.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15718..16945)
                     /gene="mdfA"
                     /locus_tag="ACHU9J_001558"
                     /pseudo
     CDS             complement(15718..16945)
                     /gene="mdfA"
                     /locus_tag="ACHU9J_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            17230..17826
                     /gene="ybjG"
                     /locus_tag="ACHU9J_001559"
     CDS             17230..17826
                     /gene="ybjG"
                     /locus_tag="ACHU9J_001559"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1905137.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            17884..18642
                     /gene="deoR"
                     /locus_tag="ACHU9J_001560"
     CDS             17884..18642
                     /gene="deoR"
                     /locus_tag="ACHU9J_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1905138.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18689..19891)
                     /gene="dacC"
                     /locus_tag="ACHU9J_001561"
     CDS             complement(18689..19891)
                     /gene="dacC"
                     /locus_tag="ACHU9J_001561"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1905139.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20138..20763
                     /gene="gstB"
                     /locus_tag="ACHU9J_001562"
                     /pseudo
     CDS             20138..20763
                     /gene="gstB"
                     /locus_tag="ACHU9J_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(20760..21875)
                     /gene="yliI"
                     /locus_tag="ACHU9J_001563"
     CDS             complement(20760..21875)
                     /gene="yliI"
                     /locus_tag="ACHU9J_001563"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1905140.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21986..22369)
                     /gene="bssR"
                     /locus_tag="ACHU9J_001564"
     CDS             complement(21986..22369)
                     /gene="bssR"
                     /locus_tag="ACHU9J_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1905141.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22582..23907
                     /gene="rimO"
                     /locus_tag="ACHU9J_001565"
     CDS             22582..23907
                     /gene="rimO"
                     /locus_tag="ACHU9J_001565"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1905142.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23954..25275)
                     /locus_tag="ACHU9J_001566"
                     /pseudo
     CDS             complement(23954..25275)
                     /locus_tag="ACHU9J_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(25283..26565)
                     /locus_tag="ACHU9J_001567"
                     /pseudo
     CDS             complement(25283..26565)
                     /locus_tag="ACHU9J_001567"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
BASE COUNT         6740 a         6902 c         6400 g         6536 t
ORIGIN      
        1 cttgatggca ttgttatccc atgccgcaga cacggccaaa aatcataaga ttgacagacg
       61 ggagttccat catgaaaaag ttagttcttg ccgctttact tgcttccttt actttcggtg
      121 cttctgccgc agagaaaatc aattttggcg tttcagccac ctatccaccc tttgaatcta
      181 taggtgctaa taatgagatt gtcggctttg atatcgatct ggcaaaagcc ttgtgcaaac
      241 aaatgcaggc agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga
      301 aattcagaaa atatgactcc gtaatctccg gtatggatat cacgcccgag cgtagcaagc
      361 aggtatcgtt taccacgccc tactatgaaa actcagccgt cgtgattgcc aaaaaagata
      421 cctacaaaac gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc
      481 accagaaata tattcaggat cagcaccccg aagtgaaaac tgtctcctat gacagttatc
      541 agaatgcctt tatcgatctg aaaaatggtc gtattgatgg ggtatttggt gacacagcgg
      601 tggtaaacga atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg
      661 atccgcaata cttcggcacc ggtctgggca tcgctgtacg tccggataac aaagctctgc
      721 tggaaaaact gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca
      781 atgaccagtg gttcccgcag taattgagac tgatgacaaa agtaagattg cctgatgcgc
      841 tgcgcttatc tggcccacat caactctgca atgtattgat ttagcatgat tttgtaggcc
      901 ggataaggcg tacacgccgc atccggcatg aacaaagcgc acctgatcag caatctgaag
      961 ccgcacctgc ggctttttta ttgcttcacc agcagcgtca tcacttcata gtgcgcggtg
     1021 tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcggt aacctggcag ttcgcggata
     1081 tctttcgcca tggtttgggc gttacagctg gagtagataa taaaacgcgg tgccatcgtt
     1141 gagagataat cacacagcgg tttaccaatg ccgcggcgcg gcgggttaac cagcaccagc
     1201 tccggcacat ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg aaattgcaaa
     1261 cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc tgatgcaatt
     1321 tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc acccacaccg
     1381 cagaacagat cccacatatg tttaactggc agctgccgta cccagtcgcg cgcggtagcg
     1441 tacagctggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg gatccacagc
     1501 ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag tcaggtagat ctccgtctcc
     1561 ccttccataa tcgccatatg taccggctga atattgacgg taataacttt cagttgcggt
     1621 agttgttcct gtaaccacgg cagcgcctta cgcagttgtg ccagtttggt ttcagaacgc
     1681 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
     1741 agttcgccac gcttacgcgc cacgttgtag ggcgttaacc ccgcacgggc gataaacggt
     1801 tttagcgccg caaaaacggg cgcaaaagag gcaggataaa gcgggcagtc acaaaggtct
     1861 tccggcgtgc catcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
     1921 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
     1981 tcaaccggaa aatcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
     2041 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
     2101 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
     2161 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
     2221 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
     2281 cggataaccg taccccagcg aagccgccca caagtaactt gcggcggtga tttgtgtcag
     2341 cagatatagc cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
     2401 aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct
     2461 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
     2521 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
     2581 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
     2641 aatgccgcgg catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca
     2701 tgtcttgcac gctggatatc gcgtatccgc tgtttttctg cgcgagccat cagataccag
     2761 gcgataaatc cgacaattcc gaccgcaccg agaattaacg ttgcaagggc gttgatttcc
     2821 ggattcactc ccatccgcac gctggaaaag accagcatcg gtaacgtggt ggctcccggc
     2881 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
     2941 ccagaaatga tcgcgggcat gatcatcggt agcgtaatga cgaaaaacac tttcagcggc
     3001 gtcgcaccga gatccatcgc tgcttcttct atcgagcgat ccagttcccg caatcttgaa
     3061 gaaataacga ccgccacata agccgtacaa aaagtgacat gcgccagcca gatggtgagc
     3121 ataccgcggt ccgcaggcca gccaatggca tgagcaagcg cgacgaataa taacaacagc
     3181 gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaaacca
     3241 tttgatccgc gaaacctgcc aaaacgcacc agcaccaccg ccgcaatagt cccgaggatc
     3301 gccgccgccg ttgccgcaca ggcagcaatt gtcaggctta aaccaaccgc actcatcatc
     3361 gcgtcatcgc gcaataactc accataccag cgcgttgacc agccggccca caccgtcacc
     3421 agcttcgagc tgttaaacga atagataacc agcatcagca ttggcgcgta gagaaaggta
     3481 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa attattcatc
     3541 cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca cattatcggc acaattagca
     3601 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
     3661 cttgccataa cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tccgggatca
     3721 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccacctttag
     3781 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg
     3841 cttccaccag cgaataatca atacgaatca acgcggtata aatcggcagt accataaacg
     3901 gcacgtaagc gtaaacaatg ccgatataaa cggccagatt ggtatgcaga atggtcagcg
     3961 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
     4021 ttcccatcca ggcataaacg cggatcagaa acgaggtcca cgacggcagg atcaccagta
     4081 gtaataaaat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
     4141 cgatcagtaa acagcaaaat gtcgaaatcg ccgccacctg taacgactgg agataagcat
     4201 caaaatagag cggatcgtcg gtcagttgca gaaaattacc gagattaaga gtgatggaaa
     4261 gttgcccgtc agcccactcc atcagttcgg tataaggtgg aatagcgcgc gccatctctc
     4321 ccaggcttat tttaaagaca atcagaaatg gcagcagaaa cagcaagatc aaccagatat
     4381 atagcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct
     4441 taaatccgcc cggcggtttc gactgggcag caggttcaag tgtactcatc tttgctcctt
     4501 aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
     4561 tcggtaaccc tttacgatga cgatgggcgt tttgtagctg ggcgctgatc atctgcccac
     4621 ttttcagtcg aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc
     4681 ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca aagcatgatt ttttccgggc
     4741 gcagcgctac gtgtaccggc acgttatcga ccactgaagc atccgcatcg actttcagtg
     4801 gatgcaccag ccccggcgaa tcaagcacca ggccatcttc ctgacgctct ttgagtacac
     4861 cttcaaagac attaaccgaa ccgataaatt cagcgctgta gcgggtagtt ggatgctcgt
     4921 agatctcttc cggctcgcca atctggacaa atttcccgcg attcataatg gcgatacgcc
     4981 ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacgc
     5041 gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
     5101 ccatcggctc atcgagcagt aatagtttcg ggcgtttggc aaggcttcgg gccagggcca
     5161 cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatgt
     5221 gcaccaggcc gagcatctca tggacccgac tggcaatttc cgctttcggt agtttgtcct
     5281 gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact
     5341 gaaacatcat attgatgggg cgcaggtaag gcggcacctg tgacaaatcg acgccatcga
     5401 gcattatctg cccggcagaa ggttgttcaa aacctgccag catacgcagc agcgtggact
     5461 tgccacagcc ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga
     5521 catcatcgac cgcatgctga ccatcgtagg atttggtgag gttgcggatt tctaatagcg
     5581 gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
     5641 tccggcattg aatgtgtgca agcgcaccat gatggtgcga attgccggct ggtgtggtgc
     5701 gcccgcccgg catctaaggc tgcggattat tttccgctct tcactttggt ccacgcgcgg
     5761 gtgcgcacac ggtcgatttt cggatcctgt actttcagag tgaatagctt cgcacgaacg
     5821 tccgccggag gataaatacc cgggttctca cggacttccg cactcaccag cggcgtggct
     5881 gctttattgg cgttggcata gaacacatgg tcggaaatat gcgctactac atccgggcgc
     5941 agcaggtaat tcaggaactg ataggcttcg tctttgtttt tggcatccgc aggcatggcg
     6001 aatacatcaa agaacgccat cgccccttct tttggaatcg agaacgagac attcactcca
     6061 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cacctgccca gccgatagcg
     6121 acgcaaatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
     6181 ttcgggcgca gctttaacag cagatcggtt gccggtccgg tgtaatcatc cgctttagtg
     6241 ctgttgggat ctttgccgag ataattcaac acggtagcaa aaacttcttc tggcgcatcc
     6301 aggaaagaga caccgcagct tttcagtttt tccagatttt caggtttgag gatcaagtcc
     6361 cagctatcga cgggcgcgtt ttcgcccagc acagctttaa ctttatcaac gttatagcca
     6421 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg
     6481 accagcttca gcagctccgg gtcgagattc ttccactccg gcaatttgct tttgtccagc
     6541 ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc tggcagatgg aaccaccaag
     6601 tcaaagccgg tgctacccgc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
     6661 tcgtagacga ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc cggggcgata
     6721 taatcagacc agttataaat gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag
     6781 acggccatca gagcacccgc agccagaccc gaaagccatt ttttatttaa ggcggtcata
     6841 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact aaaagatatg
     6901 caataaacgt gcatattttt tatcggcggg gtgtaagaaa agagaactct ctcccggcca
     6961 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
     7021 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
     7081 caaatcgaca caagagaagt gaaataatct ttaagaaaag gttaaaaaca gcagataatt
     7141 ttgcagcgcg cagccactgc accagcgact gcgtgctcga aaggagttat cagtggagga
     7201 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
     7261 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
     7321 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc agctttggca
     7381 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
     7441 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
     7501 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
     7561 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcattga
     7621 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
     7681 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
     7741 aatgtgattg cgactaacca cccgttttca ggcaatattc tgtcgtagcg tggcgttcga
     7801 tccagcggat cattttaccc gcgatgtcga taccagtggt tttttctatt ccttccagcc
     7861 ccggcgacgc attcacttcc atcaccaacg gcccgcgatt agcacgcaga atatccacac
     7921 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
     7981 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
     8041 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
     8101 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccgcggaaag
     8161 cgtcaatcac gctttccgcc gcctgacgcg tctccgccag cacgacgcca attccctgcg
     8221 tgccttcaac caacttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg
     8281 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
     8341 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
     8401 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc
     8461 caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
     8521 acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca accaggtgac
     8581 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
     8641 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
     8701 catcgacgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa
     8761 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
     8821 tattgctgcc acgggtgagg taatattccg ccagttgctc gtcatactgc gccagtgcgt
     8881 ctttatccag cggttgatag ctgttttcat gcaccaaaat ggaggccggt aaacgcggct
     8941 tgagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
     9001 gcggtaattt cagcagtttc gtcaccgctt caatattatt gcgcaggccg ccgatatata
     9061 ccccgcccaa tcccagcgat tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat
     9121 caacgacacc gagcaacagt tgttccgcca ggccgagctg agcatccgga cagatctgta
     9181 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
     9241 gcccgccggt cagcgtcacc agttcttcac gtaatgcttt gtcggtaatg cgaataatgc
     9301 tactacactg caaaaaactg gaactggacg tcgcacgggc gctgttaata atcgcctcac
     9361 actgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
     9421 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
     9481 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
     9541 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
     9601 tcaggcagcg acagggagtc gcttaccgac agcagcgcca ccgccagtag catcattccg
     9661 ataaattcca gtatcaacac gcctttaggc aatttaccga tcgcgcgcat acgcttccct
     9721 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
     9781 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
     9841 acaggcataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgttcgggtt
     9901 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
     9961 ttcagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
    10021 aggcaggtaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
    10081 gctataccga ttttgctgca tgggtgaaag aaaatctgga cgcctgatcg tctgacaagc
    10141 cctcgcgttg agggctttac tgattttttc tgtgctgtgg tttaaacaaa ctactgataa
    10201 ataagaaaca cagtgccccc agcgcacacc agaacaccgc gcttagtaac catgccagct
    10261 cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatt gaacagggtg
    10321 ccgccagcat tgcgccaaat agaggtttca ggacttctct acgctgtgaa aagaaactgg
    10381 caactgctcc aggaagaatg aaaaacagca agccgatttc aggatgcccg gcagcccgaa
    10441 aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg acaaaacagc
    10501 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
    10561 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
    10621 atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata
    10681 tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa
    10741 aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc
    10801 tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg
    10861 gtcctcgcgc ttgggctatg tctcggaaag ttacgacttg gttcgatcca actgggtaat
    10921 tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc
    10981 gatgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg
    11041 aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg
    11101 gttggcagtg cgctggtgat cgccttaggg ttaggtaagc tgtttggctg ggatattggc
    11161 ctgacggccg gtatgttagc aggctctatg acgtcgacac cggttctggt cggtgctggc
    11221 gatacactgc gtcattccgg catggaaagc aggcagctct cactggcact ggataatctg
    11281 agcctcgggt atgccttaac ctatttaatc ggtctggtga gtttgattgt tggtgcgcgt
    11341 tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa
    11401 cgtggcctgg acactgatgc caaccgtaag gtttatttac cggtgatccg cgcctatcgc
    11461 gtcggcccgg agctggtggc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat
    11521 cgacaaaccg gctgctacat tgaacgtatt cgacgtaacg ggattctggc aaatccagac
    11581 ggtgatgccg tgctacaaat gggcgatgaa atagcgttgg taggctatcc cgacgcccat
    11641 gcccgactcg atcccagctt ccgtaacggt aaagaagttt tcgatcgtga ccttctcgac
    11701 atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgtagg taaacgtctc
    11761 gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcattcg tagccagatt
    11821 gagatgccga tagatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggt
    11881 gatgcccgtc gcgtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag
    11941 gtcactgacc tgctggcatt ttgcgccttc tttgttattg ggctgatgat cgggatgatc
    12001 accttccagt tcagcacatt cagtttcggc atggggaacg ctgccgggtt gttattcgcc
    12061 ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcaaggt
    12121 gcattaagca tggtgaaaga gttcggcttg atggtgttta tggcaggcgt tggtctgagc
    12181 gccggtagcg gtattaataa cggcctgggc gcgattggcg gtcagatgtt gattgccgga
    12241 ttaattgtca gtcttgtgcc cgtggttatc tgtttcttgt tcggtgctta tgtattgcga
    12301 atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg
    12361 gagatcatca gtgatacagc tcgcagtaac atccctgcgc tgggctatgc gggcacctac
    12421 gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga
    12481 ttaggataaa actgaagttg ccctgaaaat gaaatttttt tgcacaaccg cagaactttt
    12541 ccgcagggca tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc attttgtgga
    12601 gcccatcaac cccgccattt cggttcaagg ttgatgggtt ttttgttatc taaaatttat
    12661 ctactaccct gcaaccctct caaccatcct caaaatctcc tcgcgcgata gcggcttacg
    12721 gtcggtgaca aaatgcagcg tcatcccctc aataaacgca tcaagcgcgc gggcggttcc
    12781 gggttcaaac cattgttcga gcgtttgctg actgcgctgc atccagtttt gcattaccgt
    12841 ttttaatagc ggttttcggc tagccagcgc gtagagctgg tacatcagct ccatattatc
    12901 cggcgttgca acctgtgagc tgtagatcat atcggtgata gcctggcatg cgccttgagc
    12961 atcactaaca tcgctaaaaa atgcctgata ttgccgggac ataatctcag taaaactgct
    13021 gaacgcctcc agcaacaact catcaattcc tgaaaaatag taggtcatcg atcccaacgg
    13081 taccccggca agggtagcaa ttttgcggtg cgtaacagca tgtattccgt aaagtttcac
    13141 cgcctccagc gtggcctgga taattttttc tcgccgttgc ggatcgttag cgcgacgcat
    13201 gtcatttcct ctgtctgcaa ttgtgtacaa atgtacacaa ccttgctaat gttgtcttcc
    13261 ctcttcttat tttgacgtga tctatgaccg taaattcttc acgtaatgca ttgaaacgcc
    13321 gaacctgggc gctgtttatg ttcttctttt tgccaggcct gttaatggcg tcctgggcaa
    13381 cccgtacgcc tgctatccgc gatattctct ctgtctcgat cgctgaaatg ggcggtgttc
    13441 tctttggtct gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg ttagtgaaac
    13501 gctttgggac acgtaatgtc atcctggtca cgatgtcctg cgcattgatc gggatgatga
    13561 tattaagtct ggcactctgg ctgacatcgc ccctgctctt tgccgttggt ctcggcgtct
    13621 ttggggcaag ttttggttct gcggaagtgg cgataaacgt tgaaggtgcc gccgttgagc
    13681 gagaaatgaa taaaacggtt ttgccgatga tgcacggttt ttatagccta ggcacgctgg
    13741 caggcgctgg tgtcgggatg gcactgacgg cctttggcgt tccggcaacg gtgcacattt
    13801 tattggcggc gctggtaggt atcgcgccta tttatatcgc cattcaggca atccctgacg
    13861 gtacgggcaa aaatgctgcc gatggcaccc agcatggcga aaaaggcatt cctttttatc
    13921 gcgatatcca gttgctgttg attggtgttg tagtgctggc gatggccttt gccgaaggtt
    13981 ctgccaacga ctggttaccc ttattaatgg ttgacggtca cggttttagc cctacttccg
    14041 gctcgctgat ttatgccggt tttaccctag ggatgaccgt tggacgcttc actggcggtt
    14101 ggttcatcga ccgttacagt cgcgttgccg tggttcgggc cagtgcacta atgggggcgt
    14161 tgggtattgg gctgattatt tttgtcgata gcgcctgggt cgctggggtg tctgttgtac
    14221 tctggggact gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc agcgataccg
    14281 gccccgatgc accaacccgc gtcagtgtgg tagcaacgac cggttatctg gctttcctcg
    14341 tcgggccgcc gctgctgggc tatctcggcg aacattatgg attacgtagt gcaatgctgg
    14401 ttgtactggc gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc aaacccgata
    14461 ccaaaacgcg gacggcgatg gagaatagtt gatgagcatt aaattaattg cggtagacat
    14521 ggatggtact ttcttaagcg atcaaaaaac ctataaccgt gagcggttta tggctcagta
    14581 tcagcaaatg aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc aatattatca
    14641 gttgctctct ttcttccctg aaattgctaa tgaaattgcc tttgtggctg aaaacggcgg
    14701 ctgggtagtg agtgaaggca aagatgtttt taatggcgag ctgtcgaagg atgcctttac
    14761 tactgtcgtg gaacatttgc tgacgcgccc ggaagtggaa attattgcct gcggaaaaaa
    14821 tagcgcctat acgctcaaaa aatatgacga tgccatgaaa acggtggcag aaatgtatta
    14881 tcaccgtctg gaatacgtcg ataactttga caacttagaa gatattttct ttaagtttgg
    14941 cctgaatctt tccgatgaac tgattccaca agtacaaaaa gcattacatg aggccatcgg
    15001 cgatattatg gtgccagtcc acaccggcaa cggcagcatc gatctgatta tccccggcgt
    15061 acataaagcc aatggccttc gccaactgca gaaattatgg ggaatagacg acagcgaagt
    15121 ggtggtcttt ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag gctttagttt
    15181 tgcaatggaa aatgccggca gcgcggtcgt cgcagcggca aaataccggg caggctccaa
    15241 taaccgtgaa ggcgtactgg atgtgatcga taaagtgctt aaacacgaag cgccatttga
    15301 ccaataaaaa cggcagcacg cccggcaaca caatacctga atgaaggctg cataattcgc
    15361 ggaaattaac ccgctgcatt atttcaggcg aaatgattat gaaaaattgt ctactgttgg
    15421 gcgcgctttt aatgggcttt actggtgtgg cgatggcgca aagtgtcacc ggcgatgtgc
    15481 cgagtggata caaagtggtt gtagttcctg attcagtcag cgttccacaa gcggtcagcg
    15541 tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct taccgcccgc
    15601 atccttatgc tcgccatctt gccagcgtag gtgaagggat ggttatcgaa caccagattg
    15661 acgaccatca ccattaattc gagaatgcct gatcgcacaa agcagtcagg cattttttta
    15721 cccttcgtga gaatttccca tctgtttatc ttttaaaaag ataaccatca gcgacagcca
    15781 caaaattccg ttgacaaggt tgaagagatt aaactgtccg ttgcccccgt tcagccaggc
    15841 atgtttgctg atttcaatac caacggtaaa gatcagcatt tgcagcattc ccatcgccgc
    15901 agaaaccgta cctttactca tatcgctggc aaacagggtt aatcgcacca gtcccgcatt
    15961 cgccagacca ataccgaaag cataaatact taacccggca gtcatccata aatacgcgtg
    16021 cgatgagata accgttgccg cagcagcgac caacagacca atcataatcg gccagccgcc
    16081 cataattatc acgtacggtg cggcgcgagg tcagacgcgc taacagcaag ttacccgcaa
    16141 ttaacgcccc gaaaataggc acttgcagca agccatattc atagctgctc aactgctccc
    16201 cggtaatgat gataatcggc gactgggcga tccacgccag caatggcagg ctaacgaatc
    16261 ccagcgccag cgcccccgcc acaaagcggc cgttcttcag caccagctta tagtcacgac
    16321 cgagttcttt cagtgacagt ttctcgccta tacgcgtggc ggtttcaggc atagctcgtt
    16381 gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa aacaaacatc ccctcccagg
    16441 gcagcacatg gatccacgcc gcgcccacca gcggaccaag tagcggagca atcagcgcca
    16501 cgttcgccat cagcgcggtg atcttgatac aaaccgcctc ttcgaaggat tcctgaattg
    16561 cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc ctgcaagaag cgcaacaggg
    16621 tgaattgttc aatgttttgc gccagtaata ttgccagaca ggtgacgata aaccacacca
    16681 cacccgccag catcaccgga cggcgaccaa tacgatccga cagcggcccc aggagccatt
    16741 gtaaaaacat cccgcccgcc agatacgcgg tcatcgaagt aggaacccaa tcaatgcccg
    16801 cctgatattg ttccaccacg gccaacatac cgggttggat catatcgttg ccgatatagg
    16861 ttgaaaattc gtaaagcacc agacagagag ggaaaagtaa cgcctgacgt ccaagcctgg
    16921 caccgaaagc taatttattt tgcatgcaat ttcttcgcca ataataatcg cgcagagttt
    16981 aataaaagcg cagctaacga gaaagcgaat tttgtagctg aaaccacggt taagcacatt
    17041 cttacattat tgcgagtata gctacgcttt ctttaagttt tatttaacct atgcccgtta
    17101 caatcaccca ccgtaaacag gccgcttgag ggaaataaga cgatgccgct ttacccagtt
    17161 taacctgcac tttattctca acgacttgcc tgtattggct cccttttaat cactttgcgt
    17221 cgggaagtta tgctggaaaa tttgaatctc tctctattct ctcttattaa cgcgacgcca
    17281 gactcggctc cgtggatgat ctcgttggcg atttttattg ctaaagattt gattaccgtg
    17341 gtgccgttgc tggccgcggt actttggttg tgggggctta cagcacaacg gcaactggtg
    17401 ataaaaatcg ctatcgcgct ggcggtcagc ctgtttgtgt cctggacgat gggacatctt
    17461 tttccgcacg accgaccctt tgtcgaaaat atcggctata acttcctgca tcatgcggcg
    17521 gatgactcat tcccgagcga tcacggtacg gtgattttca cctttgcact ggcattttta
    17581 tgctggcatc gcctgtggtc cggctcactt ttaatggttc tggccgtcgt cattgcctgg
    17641 tcgcgcgttt atcttggcgt ccactggccg ctggatatgc tcggtggatt gctggcaggt
    17701 atgattggct gccttagtgc ccagattatc tggcaagcga tggggcataa actctatcaa
    17761 cgtctgcaat cgtggtatcg cgtctgtttt gcattaccga tccgcaaagg ctgggtgcgt
    17821 gactgatctc tgctaaaaag tgtagtattg agcggctcgc ttcaataact attcagaggg
    17881 attatggaaa cacgtcgcga agagcgtatc gggcagctgc tgcaagaatt aaaacgcagc
    17941 gataagttac atcttaaaga cgccgccgcc ctgcttgggg tttcggagat gacgattcgt
    18001 cgcgatctga acaaccacag tgcgcccgtc gttttgctcg gcggctatat tgttctggaa
    18061 ccgcgcagtg ccagccatta cctgttaagc gatcaaaaat cccgcctggt ggaagaaaaa
    18121 cgccgggcgg caaaactggc tgcgacactg gtagaacccg atcagaccct cttttttgac
    18181 tgtggcacca ccacgccgtg gattattgaa gcgattgata atgaaatccc ttttaccgcc
    18241 gtttgttatt cgctaaatac ctttctggcg ctgaaagaga aaccccattg ccgcgcattt
    18301 ctgtgcggtg gtgaatttca cgccagcaac gccattttca accccatcga ttttcagcaa
    18361 acgttgaata atttttgccc ggatatcgct ttttattccg cggcgggcgt gcatgtcagt
    18421 aaaggcgcta cctgttttaa tcttgaagag ttgccggtaa aacactgggc catgtcgatg
    18481 gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg gcaaggtgcg tccggcgcgc
    18541 atgggtgacc tgaaacgctt tgatattgtg gtgagtgatt gttgcccgga agatgagtat
    18601 gtgaagtacg cgcagacgca ccgtattaag ttgatgtatt aatgacgtat aaccggatga
    18661 cgtttcgcgc catccggtta tcagaagatt aagagaacca gctgccgaac cactgatgga
    18721 atttcatcat cacgaaatcc cacatccgac caaagaatcc gccctcttcc acattttcca
    18781 tcacgatcag cggacgctgc tcaatggatt taccgttaag ctggaaatca atggtcccga
    18841 caacctgacc ttttttcagc ggtgcggtaa gctgcggttc cgttaacgtg tagctcgcct
    18901 tcaggttttt cagctgccca cgcggtatgg tcacggagcc cgcttcgcct gccccgagat
    18961 tcacttcgct cttatcacca aaccagacgc gctgagtcac aaaggtggca tcaggtttaa
    19021 ttggcgtcac agtttcaaag aagcggaaac cccaggtcaa taatttctca gactcattaa
    19081 aacggatacg gtcagttttc gcccccagca ctacggagat taaacgcata tcaccctggg
    19141 tagccgaagc aaccagatta tatcccgcac ctgccgtggt tcctgtcttc atgccatcaa
    19201 cattcagatt gctgctccac agcagacggt tacggttagg ctgacgaatt ttgttgaagg
    19261 tgaactcttt ctctttatga atggcgtact cttccggcac atcgtggatc aatgctttac
    19321 ccagcaatgc catatcgcgc gcggtactga actgccccgg cgcatccaga ccgtgcaccg
    19381 tctggaaggt agtgttggtc agacccagtt ttttggcata accattcatc aaaccaataa
    19441 atgactcctg gctcccggca acgtaatcgg ccagcgcaat acaggcgtca ttaccggact
    19501 ggataatcac acctttgttc aagtctgcca ccgaaacctg atcgcccggt ttgaggaaca
    19561 ttaccgatga accacgcagt gccggatttc ccgtcgccca ggcatcttta ccgaccgtca
    19621 ccatatcggt gagtttaatc ttatcggcct taagcgcctg cccaaccaca tagctggtca
    19681 tgattttagt caggctcgcg ggatccagtt tctcatccgc gttgccttct gccagcactt
    19741 taccgctggc gtaatccatt aaaatccatg cacgcgcatc cacgctcggc gcttcaacgg
    19801 tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa aaatgcagaa cccgctgcaa
    19861 gaccacgaag gagagaggag tattgcgtca taaatgccac ccgagtatcc attcaaaaaa
    19921 aatacgttat atcgccgtat gataagttcg gtgattaata acgcacacac aaacttaaag
    19981 aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat cctgccgctt cgctgcttct
    20041 ggtgtcattt ttttgccagc tgttgcttaa aagttaactt tacttcacca tcggagtcct
    20101 gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg attacgctgt ggggtcggaa
    20161 taattcaact aacgtaaaaa agtattgctg acgctcgaag aactggaact accttatgag
    20221 caaattctcg cgggccgtga gtttgggata aatcacgatg ctgattttct ggcgatgaac
    20281 cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg atctcattct ttgggaatca
    20341 aacgccattg tccgctatct ggcggcacag tacgggcaaa aacgcctgtg gatcgactca
    20401 ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg caaaccagac gctcagtaat
    20461 gctcatcgcg ggatcctgat gggattagtc agaacaccac cggaagagcg cgatcaggcc
    20521 gccattgatg ccagttgcaa agagtgcgac gccctgtttg ccctgctcga tgcggaactg
    20581 gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg gcgatatcgc tatcgcaccg
    20641 ttcatctaca atttgtttaa cgttggcctg acctggacac cgcgtccaaa tctgcaacgc
    20701 tggtatcagc aactcactga acgccccgca gtgcgcaaag tggtgatgat tcccgttagc
    20761 taattgcgtg ggctaacttt aagtaattcc ccactggact cgtcggtgag aacgtataaa
    20821 taaccgtcgg gtccagtgcg aacatcacga attcgctgcc ctctgtccgt taaaatacgg
    20881 ccatcttctg tcactttgtc gccgttgacg ctcatcacaa tgacatcttt atctttcagc
    20941 gcgccaataa ataatttttg ctgccactgg gggaatttat cgctgttata gaaggccatg
    21001 ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc ggcgacgatc
    21061 tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt tgccagcggc
    21121 cagccgtagt ttttgccttt ttgcgggata ttaatttcat caccaccgcg cgggccatgt
    21181 tcattcagcc acagtgcatt actccacgga ttcatcgcca ttccttgcgg attacgaatg
    21241 ccataagacc agatctcggc gcgcgcaccg gattccttta taaaaggatt atcatccggg
    21301 atttcgccct ggtcggtcag acgcaccagt ttgccctgta atttatccag atcctgcgcc
    21361 gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc gtcgaatacc
    21421 agccgcccgc caaaatggtt gccggtagac agttttggca tctggcggaa gacggtgcgg
    21481 aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc agttccggct
    21541 ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg agcaaaatca
    21601 ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc cggaactccg
    21661 gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc gccgcgcaga
    21721 gtgattaaca taccgtgatt atcgggtaaa aaggccagtg cccagggatg gtcgagtttg
    21781 tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga agaaagacaa
    21841 ataaggggca caaggaaaaa ggattgtcga tgcataacgc ctccggattg actctggctt
    21901 aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa acatgaaggg
    21961 ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc ttctgcaggc
    22021 tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg ttgacggtaa
    22081 taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc gctaatgctt
    22141 cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc aaccgcagcg
    22201 atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga ttgctttcgc
    22261 tgacggacat gtatcctttt gccttcctca gttgcacgta tgcagcgagg tcaacgcgtg
    22321 cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata cttcgatcct
    22381 cctctttcca gccttgttgc gattaaatag tatggctatt ttttgagcaa aatgctgtcc
    22441 aggatcaatt cttcacgcca aattaacaaa atttacattt ccttcgcctc cgccagcgaa
    22501 tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg acctttcctg
    22561 taactgaata ttttttaagc tatgagcaaa gtaactcccc agccgaaaat cggctttgtt
    22621 tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga actacgcact
    22681 gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt caacacctgc
    22741 ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc gttgaatgaa
    22801 aacggcaagg taattgtgac cggttgtctg ggggcaaaag aagatcagat ccgcgaagtc
    22861 cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct ggagcacgtt
    22921 catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc agaacaaggt
    22981 gtgaaactga caccgcgtca ttatgcctat ctgaaaattt cggaaggctg taatcaccgc
    23041 tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc gattggcgaa
    23101 gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct ggtgatctcg
    23161 caggatactt ccgcctatgg cgttgatgtt aaacatcgta ctggcttcca caacggcgag
    23221 ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg gatctggaca
    23281 cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact gatggcagaa
    23341 ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg cattctcaaa
    23401 ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca gtggcgcgaa
    23461 atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctag cgagacggaa
    23521 gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg cgttggctgc
    23581 tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca ggttccggaa
    23641 gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat ttccgccgag
    23701 cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt ggacgaagaa
    23761 ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt ttatctcaat
    23821 ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca cgccgatgag
    23881 tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca tttgtccgcc
    23941 ctgcgaataa tgattactgc cagctaatct gagccctgcc gtttttctta ctcttataca
    24001 gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt gaatgcgcta
    24061 caccgatact ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt tcttttagaa
    24121 tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg tcaccgccga
    24181 agcggtagat tttaccttta tctaccggga tctgtgattt aaggattttc accactttca
    24241 tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg aatttatcga
    24301 cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct tcgtcaaagg
    24361 attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac atactgtaga
    24421 gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt tcaaaatggt
    24481 tattaatgat ggcttctttc tccacaatac cacgattaat cactctgcgt accagataga
    24541 ggcaactggt acccgatatc actgccacca atgcggcaaa gaaaataatc aatgtacaga
    24601 cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc aaataggcga
    24661 agtgctccat cgcttccgaa acgatgaaga tggcgttttt agcattatat tcatcaccgg
    24721 tttaccgttc ttaaatagag agaacatcag gttctcgctg tccatcgcca gcgattttga
    24781 cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg
    24841 atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg
    24901 tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg
    24961 aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag agggggaaac
    25021 cgagtcggcg taagctgtgc gcaaaccaac caacagctgt tcctccgtgg gatttagcgt
    25081 gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc
    25141 agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt gagtgtagat
    25201 cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa gtacgaactt
    25261 attgattctg gacatacgta aactactctt ttactaattt tccactttta tcccaggcgg
    25321 agaatggcat gggtttctgg tacagatacc cctgagcctg aacacatcct aatttaatta
    25381 attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc cggttatagc
    25441 gtgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata tcgccggtaa
    25501 attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc aggtttgaca
    25561 aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt tgttcaatat
    25621 tgagataaaa tgttcattaa ttcgctcgat cacggtttca gtgatctcaa cacagaggct
    25681 gttcgccttc aggccatttt ctttcatgat ggtggtaaaa cgctcatgaa agtgcggttc
    25741 gtttaactgc caaggcgaaa cattaatatg cagtaagaaa tcatccttca gcgacgcacg
    25801 ctgactaaaa tgtctgaatt cacccattgc cgttttaata atctgataac ccagctcatt
    25861 gataaacccg atatcttcag caatggtaat gaacttcagc ggtgaaatga tcccccgctc
    25921 cgcagaaacc caacggcaca acgcctccgc gcccagaatg gcgcgggtgt taatatcaac
    25981 gatcggttga tagacgagga atagttcgcc aatttccatt gcatggcgaa tattacgcgc
    26041 aatccagata ttctcttcta tttcgttgag tttcgcgtca tcacagaaaa taaactcgga
    26101 gaacatatgg tcacggatac tggaaatcgc cagtttcagt ttttctgcgt aactccaggc
    26161 gttttccccc tgcaaaggtt cacaaatggc agacttatgg atgcgcagat tgcggtagcc
    26221 aaagctgctg tcaaaatcgt ttaccatcgc tattttacgg gtaaatgtct gtacattttc
    26281 ccgacacaga agtaaataga gatcggcatt gaatgtcacg atatcgcagc attgcgcata
    26341 tttctcggca attcgctgac acaatttcct gacgatttta ttgcgttcat caacaccaaa
    26401 caccgcttcc acggcttcca gaccacacag tttgatgatc cccacagcca gatactgatt
    26461 attgttggac tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat
    26521 cagtgagagc aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaa
//
LOCUS       DAZPJP010000041        26235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPJP010000041 DAZPJP010000000
VERSION     DAZPJP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.041"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(13..1035)
                     /locus_tag="ACHU9J_001568"
     CDS             complement(13..1035)
                     /locus_tag="ACHU9J_001568"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR1905143.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(1122..3419)
                     /locus_tag="ACHU9J_001569"
     CDS             complement(1122..3419)
                     /locus_tag="ACHU9J_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1905144.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(3409..3684)
                     /locus_tag="ACHU9J_001570"
     CDS             complement(3409..3684)
                     /locus_tag="ACHU9J_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1905145.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3681..3905)
                     /locus_tag="ACHU9J_001571"
     CDS             complement(3681..3905)
                     /locus_tag="ACHU9J_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR1905146.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3908..4207)
                     /locus_tag="ACHU9J_001572"
     CDS             complement(3908..4207)
                     /locus_tag="ACHU9J_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1905147.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(4207..4431)
                     /locus_tag="ACHU9J_001573"
     CDS             complement(4207..4431)
                     /locus_tag="ACHU9J_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR1905148.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4495..4995)
                     /locus_tag="ACHU9J_001574"
     CDS             complement(4495..4995)
                     /locus_tag="ACHU9J_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR1905149.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(5165..5437)
                     /locus_tag="ACHU9J_001575"
     CDS             complement(5165..5437)
                     /locus_tag="ACHU9J_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR1905150.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5574..5867
                     /locus_tag="ACHU9J_001576"
     CDS             5574..5867
                     /locus_tag="ACHU9J_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1905151.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5937..6917
                     /locus_tag="ACHU9J_001577"
     CDS             5937..6917
                     /locus_tag="ACHU9J_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1905152.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(7103..7603)
                     /gene="cpxP"
                     /locus_tag="ACHU9J_001578"
     CDS             complement(7103..7603)
                     /gene="cpxP"
                     /locus_tag="ACHU9J_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1905153.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7753..8451
                     /gene="cpxR"
                     /locus_tag="ACHU9J_001579"
     CDS             7753..8451
                     /gene="cpxR"
                     /locus_tag="ACHU9J_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1905154.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8448..9821
                     /gene="cpxA"
                     /locus_tag="ACHU9J_001580"
     CDS             8448..9821
                     /gene="cpxA"
                     /locus_tag="ACHU9J_001580"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1905155.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9927..10601)
                     /gene="yiiM"
                     /locus_tag="ACHU9J_001581"
     CDS             complement(9927..10601)
                     /gene="yiiM"
                     /locus_tag="ACHU9J_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1905156.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10750..11733)
                     /gene="kdgT"
                     /locus_tag="ACHU9J_001582"
     CDS             complement(10750..11733)
                     /gene="kdgT"
                     /locus_tag="ACHU9J_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR1905157.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11993..12613)
                     /gene="sodA"
                     /locus_tag="ACHU9J_001583"
     CDS             complement(11993..12613)
                     /gene="sodA"
                     /locus_tag="ACHU9J_001583"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1905158.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12898..13932
                     /gene="rhaT"
                     /locus_tag="ACHU9J_001584"
     CDS             12898..13932
                     /gene="rhaT"
                     /locus_tag="ACHU9J_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1905159.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13929..14777)
                     /gene="rhaR"
                     /locus_tag="ACHU9J_001585"
     CDS             complement(13929..14777)
                     /gene="rhaR"
                     /locus_tag="ACHU9J_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1905160.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14851..15687)
                     /gene="rhaS"
                     /locus_tag="ACHU9J_001586"
     CDS             complement(14851..15687)
                     /gene="rhaS"
                     /locus_tag="ACHU9J_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1905161.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15975..17444
                     /gene="rhaB"
                     /locus_tag="ACHU9J_001587"
     CDS             15975..17444
                     /gene="rhaB"
                     /locus_tag="ACHU9J_001587"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1905162.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17441..18700
                     /gene="rhaA"
                     /locus_tag="ACHU9J_001588"
     CDS             17441..18700
                     /gene="rhaA"
                     /locus_tag="ACHU9J_001588"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1905163.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18942..19766
                     /gene="rhaD"
                     /locus_tag="ACHU9J_001589"
     CDS             18942..19766
                     /gene="rhaD"
                     /locus_tag="ACHU9J_001589"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1905164.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19776..20090
                     /gene="rhaM"
                     /locus_tag="ACHU9J_001590"
     CDS             19776..20090
                     /gene="rhaM"
                     /locus_tag="ACHU9J_001590"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1905165.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            20391..20837
                     /locus_tag="ACHU9J_001591"
     CDS             20391..20837
                     /locus_tag="ACHU9J_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1905166.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20848..22299
                     /locus_tag="ACHU9J_001592"
     CDS             20848..22299
                     /locus_tag="ACHU9J_001592"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1905167.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            22289..23359
                     /locus_tag="ACHU9J_001593"
     CDS             22289..23359
                     /locus_tag="ACHU9J_001593"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1905168.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            23359..25107
                     /locus_tag="ACHU9J_001594"
     CDS             23359..25107
                     /locus_tag="ACHU9J_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1905169.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(25157..26155)
                     /locus_tag="ACHU9J_001595"
     CDS             complement(25157..26155)
                     /locus_tag="ACHU9J_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1905170.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGKESTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQY
                     QLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         6465 a         6725 c         6504 g         6541 t
ORIGIN      
        1 caggccattt tatcattcta aatggttctt gattgattgc ccaattgctt ttcctagttc
       61 cacaggaaca gcattaccta tcattttccc aatacttctt ataacaaact tgtttttgtc
      121 ttctacaaaa atataatcca tcgggaaagt ttgcaataaa gcagcctctc taagagaaat
      181 cgctctatgc tgttctggat gtccaaaacg cccattcccg aaaccataac aaagagttgt
      241 aattgttgga cttggcttat cccaagacat gcgcccatat acactagcat accctttacc
      301 gctggatttc atatgacaag cggcaaccaa ctctttcggc cagtccttcc atgtaccacc
      361 aggaacagag tgtataattc tttttttatt aatcggattt aatttactgg ctctatgtaa
      421 tatatcattt ggatctgttt ggccatcttc taatggagga agatttccta ttgcatctct
      481 aactgttttc aaaataacat cgtcacgttt tactaactca atcgccccca attttgaggc
      541 caacaataca tgacgggaac ggttttgtgg gattccatat tcgacacaat caatcttaga
      601 cgcccaaaca taatagccca actttaacaa tgaattataa aaatcattat aaactttatg
      661 tttagttaca tcaggaacgt tctccattgt tacgatttct ggagatactt cgcgaattaa
      721 acgttcgaac tcataaagaa gaggccattt tttatcatca gccttatctt ttccttgagt
      781 atattttgaa aaaggttggc aaggtgcgca tcctgccaat acttttacag acgcatcacc
      841 ataaagcctc agcagttcct cttttgttac tttagcgata tcttgctcaa taaacttaga
      901 tttattgttt ctttcatagg gaaacctaca ttccccctca aggtcaatgc ctgcaacgac
      961 atcaagcccc gcaagctgca aaccatgagt taatccgcca gcaccacaaa aaaggtctat
     1021 cacctttaca gtcatcactc actccaaatc tatacgatac tcagtataca tccctttcta
     1081 caaaatctcg aggcctaact tataaactca tatttattga gttaacgctt cactgagttt
     1141 gactctaata ctgttgagaa tcccgaccac tcatcagcga gcgggtaagt gaattttttc
     1201 ccgtcataat ttacggttgc cccacgcgcc agcacctcaa gctcccatcg ctgcggcctg
     1261 ataccgttct gagcaaggtc aacgcggata cgggtaattt gcattcgttc cgaccgggtc
     1321 agtctggccg atggcgctat ttcatgcggt tttaacgggc ttccgtttct ttgctgacgg
     1381 tttggtcttc tcagtccgtg ttttaatgca cctctgagcg ccctcacaac ctccgggtca
     1441 ttccattcga taacaccgtc atctaccaga ttaagcactg ctgcggcgtg ctcagaaggt
     1501 gtgggagccg gtaacgaagt atcaccacca gtgagctttc cacagttatt gacaggactc
     1561 cgaggcgcgg cgatgccgct ttttaaagtc aaaggctcaa cgaccggcac tttcggaaca
     1621 atgcgccagt ccgtcgttct ggtgatatga atatgacgcg cgccgagatg cggcgcgtaa
     1681 atgccgacca ctctctcgac ttcttcctcg tactcgttaa cttcatccga cggactacgg
     1741 gcgaccctga cagtctgaca atcgcgcggg acatttgccc caccctgcgc gctgatatac
     1801 agcgcaaaat caccactgtc tgcggcggcg cgtgcagcct cgacgcgctc gtcaaactca
     1861 tcagcaatgc tgacgccgcg aggcaatttg cgtagttcac ggtaagcccc cattgtcggc
     1921 aggccaaccg ttttaaattg cggaatgcgc cacgttgacg cccatgcggt aacagccgcg
     1981 gcagtatctt tcagcggtct gccgctatcg ttatcgagct gcccatccag tgcatagccg
     2041 tcgatatttt ttgagatgta tttcgcgata taccccgcag caccgccccg attaagatgt
     2101 tttgcctgaa aacggtttcg cgcagctcct ctttcgtcgc catcctcttt gagcgcgtag
     2161 cgacgcatga tttcgataat ctggttacgc tggcgtggat tacaaaaaag catcatatgc
     2221 cagtgcggcg ttccgtcgtg gtgtggctcg acgactcgca aaccgtagac ctgtaaatca
     2281 ttatccttga atgccgtgcg catcaggctc cagatacggc agagataacg ctgcgcatcc
     2341 tttggattaa atgcctcatc gttccagccg tgatttagct ggacggtttt actttcgcct
     2401 tttccaacct gacgtgtcgg gtgatacttt gacggcgcgg tcagcgtgat aaacatcccc
     2461 acatcacctt ctgcggcggc gtaacgctca ataccggcaa tggtgttcat cagctccatc
     2521 cggcgaattt caggattaga aatactgccc atcaccttac tgataaggtc gatgcgctcg
     2581 cctgtttccc tgttttcgag atcacacgat ttaagaaatt ccagatttgc ctggcggcgt
     2641 gcacgcacat cacgaatggc atgtttactg gcataaggag aacggtcttt attgacctcc
     2701 ccgacagcaa tcagtaacgc ctcatgccag cgcatacgct ggcctttaag ctgactaatc
     2761 caccactcat cgttaaacag acgggcaatg gcagaatatg cctgcctcgt ggtcatctgt
     2821 cctttacggt attttttcca gtagagaggg gaaatattga aagcacgtgc agcgccagca
     2881 acatgaccat agaggtgagc ctgcgcctca tccgtaaaca gcgattcttt ttcgccatgc
     2941 gcatccaccc aggcatcgct gagttcctca tacatcatga aaagctgcga tgagatacgg
     3001 gcggcaaact ttttcagctc cttgtcattc attcccggca ggcgcgcata gtggtcacgc
     3061 tcagccagaa acagcatcga cgcgtcggtg ttcatttcat ggcgctgatt cacacgctca
     3121 atgcgcggcc ataaacgacg ctgaaaagtg gatgtgagga aataaaaccc gtgcaccggg
     3181 cttttattgc gccggatgta gtcatagcgt gacgtaaaca gcgagcgcaa aaagtaaggc
     3241 aggcggttaa tcgtggataa aacaccttgc acctgacgca tctcgtcacg tgtaaggggt
     3301 ctttcgcgcc cgacagcctc gcgtggcgcg ttccatgcat aagcaccggt aaacgcctta
     3361 ccggtgcctg cagcaaatgc tgacggaggg acaaaacgcc cggaggcttt aacggccata
     3421 tgagccaaaa gcctctgaac aacgcttgct gagttgctca acctgcgcgt ttaaatcagc
     3481 aaaagacttt gcgcttccgg tcagaatatc gtgatgcatc aggccggaaa cgagctggct
     3541 taatttcggg taataaccaa ccaccgccag ccattcctga ccggcgtttt taccgctttc
     3601 cgctctcttt ttctcgtgga gaataaactg gaagctgtca ctggtaacga cataacgttc
     3661 gccaatttca atacgaatac tcatgccgtt ctccggtaat gtttgttttt tgcttcaaag
     3721 actgactgac aggaaacaca acgcgtggct gacggataag ccgcacgacg ggcagcagga
     3781 attggcgcgt cacactcttc gcaaaccagc gcagaagcac cgcaatgttt tacccttgcc
     3841 gcgttaatct ggcgctccag taattcagcc tgttgttcct gaataaaatc tatgttgtcc
     3901 ggcattatca gctcctttta tcgttaagtt tcctggatac atcagcgcaa taactggcaa
     3961 gttctgtcgt taattttgtc agttcatcca ctgaggaaat ttgcttgtgg aatacagcgc
     4021 gtttaacaag taaattgacc acatcagaca ggaggtttaa ttcattctga taaatcgcga
     4081 taacagattc agtgatgtcg cgtttttctt tatcaagaca aagttgaata agagacaagt
     4141 cgccattttt cataacggcg atttttaagg cgttattcag taatacaact gaatgagaac
     4201 aggacatcaa agcacctccc cgcgagacaa tccgatattg tgaaattttt ccgactcctg
     4261 actgagcagc tcgactatct ccacgcggga taactccgcc tttgtgatat ggcgaatcat
     4321 ggcgtcaaga tgagaagaaa agcgcgtcgc agcgtcggcc tgtgcttcgg ttctggcctg
     4381 ttgcagcagt aatgcgtatt taccgcactg attttcagaa actgtatgca tgactttctc
     4441 caggcaaaaa gaagccccgc acaattaagt gcgttaaaaa ctctggttaa ttatttaatg
     4501 caaatattgc tctggtttta ccgacgtcag aattgtcggt gcatactcaa acagactgaa
     4561 tagctcacgt aatgcacgga ataaagcatc acgccagtaa catgactctt cattaattcg
     4621 ccagtatggc tgattgaatt ctttttcagt caatccggca tgcataaata aagtacgacg
     4681 ctgactgact gttaaaaaac taatatatgc atactcactt gcgccaacct gacggcgttt
     4741 tgagaatgcc ccacgcaatt catcaattgc acaaaccagc cgttcacgtt cgacgtcgtt
     4801 catttcttca aaacgcatcg ttgcgtgacg ttgttttaac tgcgcatgaa agcaaactgt
     4861 tagccgttcg cgctccatca tctgattata ataatcacat gtatcctgcc aacgagggac
     4921 ggcaagatgc ttaccaatta tccggcgcat agctgctggc tgtttttcga cgagattgag
     4981 cgtcatcact gtcatttcca gaccctccgg cttttcagaa aggtcagagc cttttttaac
     5041 ggactctgtt ttttggtgcg gataatgatt cccttgcgac ccttcccgtg ggtgatggtg
     5101 aagtcaatcg ccctagggct ttcgttacgc aataactgag caatacaacg cggctcattc
     5161 ataatcacaa ccccatccac aaaagccatg catcacgctg ttcaaccggt cggttataaa
     5221 acgcctcacg tacagcgcga ttaaactcag gaatgaaaac ccatttttca ccggcacgag
     5281 ccttcggttt gcaaggatca cgcaattcaa taattggtaa tttatttgcc ttcaccattt
     5341 cactgacggc tgtctttggc ttccctaata aatcagcaaa tttatccaca tgaaccgcat
     5401 caagcggata ctgaatcaca taattttcag cgtccatata tggtaccctc ataggatcca
     5461 gccctttcta aaccactcaa aaccgtttag acgctggttt attctcaaat caatggaacc
     5521 tatataggtt ccagttttga gggaatttag tccctatata ggcaccatgt caaatgaaat
     5581 taagcgaaaa gattaaggcc ttgcgtgagg ctgaagggct aagccaatca aaattctgtg
     5641 aaatcataga gttaccgcta agcacactta aaaaatatga aggaggaaac tttgaacccg
     5701 gtggcacagc tttgctaaaa atcactatgc atcccacatt ccaaaaatat gctctatggc
     5761 ttatgacaga taaaaccgcg ccggacgcag gacaaatcgc accggctctc gcgcacattg
     5821 ggccagagtc aacagagtcc aaccactccg cgaaaaggat tggctaactc tatataaaga
     5881 ttacattttc accatttgct accaagatgg tgaatacagc gccggagggc tttcttatgg
     5941 caattaagaa gctcgatgat ggtcgctatg aagtggacat tagacctcgc ggtcgcgacg
     6001 gaaaacgcat ccgcaggaaa ttcgaaagaa aagctgaagc actagcattt gagcgataca
     6061 caatcgccaa tgccagtcag aaagaatggg gaggccagcg agcagaccgc cggactttga
     6121 gtgagttgct ggacatctgg tggaaatatc acgggcaaaa ccacgagcat ggaacaaaag
     6181 agtttaatca tctactcaaa accatcagcg gcataggtga tataccagtg agcaggatga
     6241 gcaaaagggc tttgatggat tatcgttcca tgcgactacg tgatggtatc agtgccgcaa
     6301 cgataaaccg tgacatgtac agattatccg gcatgttcac aaaattaatt caattggatg
     6361 aattttccgg gcaacaccca attcacggac tgccgccact ggcggaggcc aaccctgaaa
     6421 tgacgttcct ggaaaaagca gaaatcgaaa aactgttaaa tgttttggct ggtgatgact
     6481 tacttgtcgc gcttttatgt ctgagcactg gaggaagatg gacgaaagtt gccacgctaa
     6541 aaccagcaca gattacaaat tgcagggtta ccttcctgaa aaccaaaaac ggtaaaaagc
     6601 gaaccgtgcc gatttctgag gaactggaga aaaaagttaa agaggaggcc agcgccaaat
     6661 tattcaaagt tgattatgag aaattttgcg ggattttacg cagagtgaaa cctgatatac
     6721 cacccaatca ggcaacccac atcctgcggc atacattcgc aagccatttc atgatgaatg
     6781 ggggcaatat aatcgcactg caacagattc tgggacatgc gagcattcag cagacgatgg
     6841 cctatgcgca ccttgcgcct gactatctgc aaaatgccgt cgctctgaat ccactaaaag
     6901 gcggagtgac gttataaatt tccccctctg agtgtccaca tagtgtccac actcaagaaa
     6961 ttttgaaacc gctccaaacc cttacagctt tcgataagta actgttttta cttttgattg
     7021 catgtaagta tttgataaaa aaaaccccac atcatgtggg ggaagacagg gatggtgtct
     7081 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
     7141 acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt
     7201 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
     7261 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
     7321 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
     7381 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
     7441 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt
     7501 cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt aatgaactga
     7561 ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc tcccaaaatc
     7621 tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat gcgtcagggg
     7681 gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt ctgcctcgga
     7741 ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct gacttcccta
     7801 ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga tggggaacag
     7861 gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat gatgccgaag
     7921 aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc tgtcattatg
     7981 ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg cgcagatgac
     8041 tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc
     8101 cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac actggaagtt
     8161 gatgccttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca aacgctggag
     8221 ttaaccggta ctgagtttac cctgctctat ttgctggcac agcatctggg tcaggtggtt
     8281 tcccgtgaac atttaagcca ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct
     8341 attgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg
     8401 tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg ataggcagct
     8461 taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg atgttggttt
     8521 tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc gaacagcgtc
     8581 aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg cccaacgatt
     8641 taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt
     8701 tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa atgcagatca
     8761 ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa aagtatggcc
     8821 gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac caactttatc
     8881 tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt gaccgcccgc
     8941 tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg ttggcctgga
     9001 gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc cagggaaact
     9061 tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca ggtgccagtt
     9121 ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc tcagcagcgt ctgctttctg
     9181 atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg gcgttactgc
     9241 gccgtcgtag cggtgaaagc aaggaactcg agcgtattga aaccgaagcg caacgtctgg
     9301 acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta
     9361 gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag
     9421 ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg
     9481 gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt
     9541 cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc attacggtgg
     9601 acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt ccgttctatc
     9661 ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg gcgattgttg
     9721 aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg ctgggcggtt
     9781 tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat ttgtaggcag
     9841 gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc
     9901 gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga aaaatcttcg
     9961 atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga taacccggcg
    10021 gcggagagta aacggtgata ctgatcgtca tcaaacggca tatgccatgc gatggcggca
    10081 gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc gtccgcagac
    10141 actttccctg gcgctatcac gctatacaac caccccacct tacctgtgtt ttgcatcaac
    10201 tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg
    10261 ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatatgaac attactttcc
    10321 gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa ctccgcctgt
    10381 tccggaaatt cccgcaccca gtagagatag tgctcacgag gataatgaca cagcgctctg
    10441 tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc cagctccgtc
    10501 agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc ttcgggataa
    10561 gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct ctccacgaga
    10621 aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc
    10681 caggcaggaa ctcaatagca aaccggacga acgttagttc atccggctgt ggggattaag
    10741 gaagcaggtt tatttcaccg tacctaaaat ttcgattttc gctgctctgg ctttgacttt
    10801 acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc
    10861 taccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt ctgcaatcag
    10921 cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc
    10981 gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga taattactgc
    11041 cacacccaac aggatcccca gcaaaccagt ctgggcaatc acagtcaaat caatggtatt
    11101 gcccagcgcg aaggcaaaga atggaatcag cgtttgcact gctttgctga aaaattctcg
    11161 taattcaggg tcaagattcc ccagggcaaa gcccaccagg aacggtaata ctgcgccgac
    11221 gaaaacatgc ggttcaaacg aggcaatccc ggcagtgccc agaataatca tcgtcatgag
    11281 cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttctttcg taccgtactg
    11341 ctgcatgatg gaagcgtaaa gtccgccgtt ggtcatatcc atcgccgcca ccagcgccag
    11401 cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa tgcgcgaggc
    11461 aattgccgca accacccacg cgacggcaat tttagttacc accagcgtac cggatttacg
    11521 cagtaccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc
    11581 cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc
    11641 ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca tcatcccccc
    11701 cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa
    11761 gggctgatgc gtaattcctc aatggagcat cagtgttgat ggaaacgaag atagacattc
    11821 atttaatcaa taaaagtgat caaaaccaca tcaattgaaa cgctgtttta tttttatcgg
    11881 atcattgttt ttattttgaa attgatcaca aaaaaaccac cgccgttggc gatggttcat
    11941 tttttaagct gatatgcggc ctatacgcct cattgcagca ggcggcaaat gattattttt
    12001 tcgccgcaaa acgtgccgct gcttcgtccc agttcaccac gttccagaac tctttaatgt
    12061 agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca tccaggccca
    12121 gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggagaa tcctggttag
    12181 cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa
    12241 agcgggaagc tgccgctttt tcaaattctg ctttgaagtt atcaacggag ccgaagtcac
    12301 gttcgatagc cgctttcagg tcaccctgca gggtggtgcc ttttttcaga cctttccaga
    12361 acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg
    12421 gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat tctggcaggc
    12481 tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg tggtggattt
    12541 ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc agggatggca
    12601 gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat gccgcgtaag
    12661 cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg ccgtactttt
    12721 cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg
    12781 ccgtgattgc cagtaaatcg acaacggcgg caacaggcga aaggttaatc gacagcacga
    12841 tttttacact catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga taagaagatg
    12901 agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag tgcagcctgt
    12961 ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt
    13021 gggattgttt cgtggattat tctgccgtgg accatcagcg ccctgttact accggatttc
    13081 tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct gttcggcgct
    13141 atgtggggga tcggtaatat caactatggc ctgaccatgc gttatctcgg catgtcgatg
    13201 ggaattggca tcgccattgg cattacgttg attgtcggta cgctgatgac gccaattatc
    13261 aacggcaatt tcgatgtgct gattaacacc gaaggcggac gcatgacgtt gctcggcgtt
    13321 ctggtggcgc tgattggcgt agggattgta actcgagccg ggcagttgaa agagcgcaag
    13381 atgggcatta aagccgaaga gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc
    13441 ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc
    13501 gctgccgcac ttggcgtcga tccactgtat gtcgctctgc caagctatgt tgtcatcatg
    13561 ggcggcggcg cgatcattaa cctcggtttc tgttttattc gtctggcaaa agtgaaggat
    13621 ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcattcacaa tgtgttactc
    13681 tcggcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg gggccacgcc
    13741 cgcattccgg cgcagtatga ctacatcagt tggatgctgc atatgagttt ctatgtattg
    13801 tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca atacaggacg ccgtccggta
    13861 acggtgttga gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc
    13921 atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt
    13981 tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata
    14041 tcactgatta acaggcggct atgctggaga agatattgcg catgacacac tctgacctgt
    14101 cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg caaaacgcgc
    14161 tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc
    14221 gccaaccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca
    14281 ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt
    14341 tcgttagcaa acgacacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc
    14401 gcctgcgcca tccccacgct acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat
    14461 cccggaatcg ctccctgcca gtcaagattc agcttcagcc gctccgggca ataaataata
    14521 ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatataa
    14581 aagagatcgc cacgggtaat gcgataagga cgatcgttga gtacatgcag accattaccg
    14641 cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cggcaaagac atcttgcgga
    14701 taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt
    14761 tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata
    14821 tggcgtacaa atacgttgag aagattcgcg ttattgcaga aagccatccc gtccctggcg
    14881 aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc
    14941 gctgaatcca cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc gtagcagatg
    15001 tcgggctttc atcagtcgca ggcggttcag gtatcgctga ggcgtcagtc ccgtttgctg
    15061 cttaagctgc cgatgtagcg tacgcagtga aagagaaaat tgatccgcca cggcatccca
    15121 attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc
    15181 gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta attgcataaa
    15241 caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg
    15301 tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata
    15361 ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc
    15421 cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg
    15481 atcatgagcg cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa
    15541 cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc
    15601 aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac cagacggaaa
    15661 aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat
    15721 agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt
    15781 cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa
    15841 tgaagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc
    15901 ctgtcagtaa cgagaaggtc acgaattcag gcgcttttta gactggtcgt aatgaaattc
    15961 agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg
    16021 ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc
    16081 atcgttttaa caatggcctg catagccaga acggttatgt cacctggaat gtggatagcc
    16141 tggaaagtgc cattcgcctt ggattaaaca aggtgtgcga ggaagggatt cgtatcgata
    16201 gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg
    16261 tgggcctgcc cgttgcttat cgcgatagcc gctccaatgg cctaatggcg caggcacagc
    16321 aacaactcgg caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata
    16381 cgctttatca actgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc
    16441 acgctctgct gatgccggat tacttcagct atcgcctgac cggcaagatg aactgggagt
    16501 acaccaacgc cacgaccacg caactggtca atatcaatag cgacgactgg gacgagtcgc
    16561 tactggcgtg gagcggggct aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg
    16621 tcattgggca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc
    16681 atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgct gcttatctct
    16741 cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg
    16801 cgctggcagc caacataacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa
    16861 atattatggg cttatggctg cttcagcgag tgcttcagga gcggcaaatc aacgatctcc
    16921 cggcgcttat cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg
    16981 acgatcgctt tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa
    17041 tggcgcaacc gctcccggaa agtgatgctg aactggcgcg ctgcattttc gacagtctgg
    17101 cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct acgcggtgaa gatttctcgc
    17161 aactgcatat tgtcggcggc ggctgccaga acacgctgct caaccagcta tgtgccgatg
    17221 cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc gacgctcggc aatatcggca
    17281 tccagttaat gacgctggat gaactcaaca atgtggatga tttccgtcag gtcgtcagca
    17341 ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg
    17401 cgcagcttca ctcgacacga cagacaaagg agctttgcgc atgaccactc aactggaaca
    17461 ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct
    17521 gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg
    17581 ttttgaaaac ccggaaggtt cgctgaccgg tgggattcag gctactggta attatccggg
    17641 caaagcgcgt aatgccagtg agctacgagc cgatctggaa caggctatgc ggctgattcc
    17701 ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagacacgc cagtctcgcg
    17761 cgaccagatc aaacctgagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct
    17821 cggtctggat tttaacccct cctgtttctc gcatccgcta agcgccgatg gctttacgct
    17881 ctcgcatccc gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg
    17941 cgtttcggcc tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc
    18001 ggatggtatg aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc
    18061 actggatgag gtgatcagcg agaagctgga tccggcgcac catatcgacg ccgttgagag
    18121 caaattgttt ggcattggcg cagagagcta cacggttggc tccaatgagt tttacatggg
    18181 gtatgccacc agccgccaga ctgcgctgtg cctggacgcc gggcatttcc acccgactga
    18241 agtgatttcc gacaagattt ccgccgccat gctgtatgta ccgcagttgc tgctgcacgt
    18301 cagccgtccg gttcgctggg acagcgatca cgtggtgctg ctggatgatg aaacccaggc
    18361 gattgccagt gagattgttc gtcacgatct gttcgaccgg gtgcatatcg gcctcgactt
    18421 ctttgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa
    18481 agccctgctg cgtgcgttgc tggaacctac cgctgagctg cgcaagctgg aagcggcggg
    18541 cgattacact gcgcgtctgg cgctactgga agaacagaaa tcgttgccgt ggcaggcggt
    18601 ctgggaaatg tattgccaac gtcacgatac gccagcaggt agcgaatggc tggagagcgt
    18661 gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa acactgccgg atgcggcgcg
    18721 agcgccttat ccggcctaca ggtcgaatga ccatgcgtgc tgccaccctc accccggccc
    18781 tctccctgaa agggcgaggg agaaaaacat agcctcgtgc ggcaacggag ctgaacaccg
    18841 ctccggacag tcccctcgcc cctacgggga gagggttagg gtgaggggaa caagccgcac
    18901 catgtctaaa caaggcaacc taatttacag gaacacagaa catgcaaaac attacccagt
    18961 cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg
    19021 agcgcaatgg tggtaacctg acgctgcgcc tggatgacgc cgatatcgcg ccttatcacg
    19081 gcaactttca tgcacaaccg cgctatattc ccctcagcca acccatgcct ttactggcaa
    19141 atacgccgtt tattgtcacc ggctccggca aattcttccg taacgtccag cttgatcctg
    19201 cagctaactt aggcgtcgta aaagtcgaca gcgacggcgc gggctaccat attctctggg
    19261 ggttaaccaa cgaagccgtt ccaacttccg aacttccggc tcacttcctt tcccactgcg
    19321 agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac gccaccaacc
    19381 tgatcgccct cacctatgta cttgaaaacg acaccgcggt ctttactcgc caactgtggg
    19441 aaggcagcac cgagtgtctg gtggtattcc cggatggcgt tggcattctg ccgtggatgg
    19501 tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg
    19561 tgttgtggcc cttccacggc gtcttcggca gcgggtcgac gttggatgaa acattcggtt
    19621 taatcgacac cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg atgggcggca
    19681 tgaaacagac catcagccgt gaagagttga tagcgctcgg caagcgcttc ggcgttacac
    19741 cactagctag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc
    19801 aggttaaccc cgacgcccac gaagagtatc agcgtcggca taaccccatc tggccggaac
    19861 tggaagcagt gttgaaatct cacggtgcgc ataactacgc catttacctc gacaaagcgc
    19921 gtaatctgct gtttgccacg gtagagattg aatctgaaga acggtggaat gcggtagcca
    19981 gcactgatgt ttgccaacgt tggtggaaat atatgaccga tgttatgccc gctaacccgg
    20041 ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccgtaa ttgcccaatg
    20101 tgccagagat gcctccggca caatatttta aatttcgatt tgaaaaatta aacatattac
    20161 atctcccggt aagttatatt tccctgatac attgtgagta aatcacaaaa ataatgaata
    20221 acccattaat gattcatgtg gtttatttaa ataaccatta tgtgcattac tccgcaaatc
    20281 tgacctttca ctctgtctca aattgtcatt gtttaccgac atatcgcctc tcatcactta
    20341 atcgctccac cattacaatt gattaaaata tattttatgg agtccgatta atggcagctc
    20401 ttactgcaag ctgtattgac ctgaatattc agggcaatgg cgcttattcc gttctgaagc
    20461 agttggcgac aatagcgtta caaaacggtt ttatcaccga ctcacaccag ttcctgcaaa
    20521 ccctgctgct gcgcgaaaaa atgcactcta ctggatttgg ttccggtgtc gccgtgccgc
    20581 acggtaaaag cgcctgcgtt aaacaaccgt ttgtattatt cgcccgcaaa gcgcaggcta
    20641 ttgactggaa agccagcgat ggcgaagacg tcaattgctg gatctgcctc ggcgtgccgc
    20701 aaagcggcga agaggatcag gtcaaaatca tcggcacact gtgtcgcaaa attatccacc
    20761 aggattttat tcatcaactg caacagggcg acgccgacca ggtgcttgcc ttgttaaatc
    20821 aaaccctcag ctcataagga agtggcgatg gagtcatcct tatgtattgt cgcgatcacc
    20881 aactgccccg ccgggatcgc tcacacctac atggtggcgg aagccctgga acagaaagcc
    20941 cgttctctcg gccataccat aaaagtggaa actcaagggt ccagtggcgt tgaaaaccgc
    21001 ttatccagcg aagagattgc cgctgccgat tacgtcattc tcgctaccgg gcgtggcctg
    21061 agcggtgatg atcgcgcgcg gtttgccggg aagaaagttt atgagattgc catctcccag
    21121 gcgttgaaaa atatcgacca gattttcagc gaattaccga caaactcgca gctttttgcc
    21181 gcagatagcg gcgtgaagct tggcaaacag gaagtgcaaa gcggcagcgt gatgagccac
    21241 ctgatggcgg gcgtctctgc ggcgctgccg tttgtcatcg gcggtggtat cctggtggcg
    21301 cttgccaaca tgctggtgca gttcggtttg ccgtataccg atatgtcgaa aggcgcgccg
    21361 tcgtttacct gggtagttga atccatcggt taccttggct tcacctttat gatccccatc
    21421 atgggcgctt atattgcctc gtcgattgcc gataaaccgg ctttcgctcc ggcgtttctg
    21481 gtttgctatc tggcgaacga caaagcgctg ctcggcaccc agtcgggtgc gggattcctc
    21541 ggcgcggtgg tgctggggct ggcgattggc tatttcgtct tctggttccg caaagtgcgc
    21601 cttggcaaag cattacaacc gctgctcggt tcaatgctga tcccgttcgt tactctgctg
    21661 gttttcggcg tgctcactta ctacgttatc gggccggtga tgtccgacct catgggcggg
    21721 ctgctccact tcctgaatac cattcctcca tcaatgaagt ttgcggcagc gtttctggtc
    21781 ggtgcgatgc tggcgttcga tatgggcggc cccatcaata aaaccgcctg gttcttctgc
    21841 ttctcactgc tggaaaaaca catttatgac tggtacgcca tcgtcggcgt tgtcgcgttg
    21901 atgccgcccg ttgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag
    21961 gaaaaagagg ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct
    22021 ataccttacg ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggtggc
    22081 attaccggcg tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc
    22141 tttgacccgc tgatcggcct gatgtcgccg gtgggttcgt tttatctggt gctagcgatt
    22201 ggcctcgcgc tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa
    22261 gcgaaagccg cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga
    22321 agccagcgcc gtcagcggcg atgaacagga agttcgcgac attctgataa acacgctgga
    22381 accttgcgtt aatgagatca cctttgatgg tctgggcagc tttgttgccc gtaaggggaa
    22441 taaaggtcca aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca
    22501 catcgacgag agcggttttc tgcgctttac caccattggc ggctggtgga atcagtcaat
    22561 gctcaaccac cgggtaacca tacgcacaca caagggattt aaaatccctg gtgtgattgg
    22621 ttccgtcgca cctcatgcgt taacggaaaa gcaaaagcaa cagccgctgt catttgatga
    22681 gatgttcatt gatattggcg cgaacagtcg cgatgaggtg gaaaaacgcg gtgtggcgat
    22741 gggcgatttt attagcccgg aagccaattt tgcctgctgg ggcgaagata aagtggtcgg
    22801 caaggcgctg gataaccgca tcggctgcgc aatgatggct gaactattgc agacggtgaa
    22861 taatcccgaa attacgctgt atggcgtcgg cagcgtggaa gaagaagttg ggctacgcgg
    22921 ggcacaaacc tcggcggaac acattaaacc ggatgtggtg attgtgctgg ataccgctgt
    22981 cgcaggtgat gttccgggca ttgataacat taaatacccg ctgaaactgg gccaggggcc
    23041 ggggctgatg ctgtttgaca agcgctactt ccccaaccag aaactggtgg cggcgttaaa
    23101 aagctgtgcc gcacataacg atttaccgct gcaattttcc accatgaaaa ccggtgcgac
    23161 ggatggcggg cgctacaacg tgatgggcgg cgggcgtccg gttgttgcgc tgtgcctgcc
    23221 aacccgctat ctgcacgcta acagcggcat gatttcaaaa gccgattacg atgctctgct
    23281 cacgttgata cgggattttc tgaccacctt aaccgcggag aaagtcaacg cgtttagcca
    23341 gttccgtcag gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag
    23401 caacagccgc gcacgcctgg tgagctggcg caacagactg gcgtttcagg caggaccatc
    23461 ctgcgtgata ttgactatct caactttacc cttaacggca aagcccgcat tttcgccagt
    23521 ggcagtgcgg gctatcagct ggaaatcttc gagcgccgca gcttttttca gttgctgcaa
    23581 aagcacgata acgacgatcg gctgctggcg ctgttattac tgaatacctt tactccccgt
    23641 gcgcaactcg cctcggcgct taatttgcca gaaacctggg tagcagagcg tctgccccgg
    23701 ttaaaacagc gttatgaacg cacttgttgc ctggccagtc gccccggttt gggccatttc
    23761 attgatgaga cagaagagaa acgcgttatc ttgctggcga acttgctgcg caaagatccg
    23821 tttttaattc cgctggcggg cgtaacacga gacaattttc agcatttatc cacggcctgc
    23881 gacaaccaac accgctggcc gctcatgcag ggtgattatc tctccagcct gattctggcg
    23941 atttacgccc tgcgtaatca actgaccgat gagtggccgc aatatcccgg taacgagata
    24001 aaacaaatcg ttgaacagag cggcatgttt cttggtgata acgctgtaag aaccctgaca
    24061 ggtttgatag agaaacagca tcagcaagcg caggtaattt cagccgatca tgtgctgggg
    24121 ttgctgcaaa gggtgccagg catcgcgtca ttgaatatta ttgatacgca gctggttgag
    24181 aatattaccg ggcatttatt acgttgcctt gccgcaccgg tgtggattgc cgagcaccgc
    24241 caaagcagca tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac
    24301 tttattacgc tactgcgtga acagctcgat attccccttt tcgatagcga tctgatcggt
    24361 ttgtattttg cctgtgcgct ggaacggcat caaaacgaac gccagccgat cattttgctc
    24421 tcggaccaga acgcgattgc cactattaat cagctcgcca ttgagcgcga tgttttacat
    24481 tgtcgggtga ttattgcccg tagcttaagc gagcttattg ccattcgcga agagattgag
    24541 ccgttattga tcattaacaa cagccattat ttactggatg acgcggtaaa taattacatc
    24601 accgtaaaaa atatcattac ggctgccgat atcgaacaaa taaaacactt tctggcgacg
    24661 gcatttattc gccaacagcc ggaacgcttt ttctctgccc ccggaagttt tcattattcg
    24721 aatgtacgcg gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtggca
    24781 caacaccata ttaccgccga tgaagcgcaa cggatcatcg cccgcgaagg cgaaggtgaa
    24841 aacctgattg ttaatcgcct cgccatccca cattgctgga gcgaacagga gcgacgtttt
    24901 cgtggatttt ttattaccct cgcccaacca gttgaggtga ataacgaagt cattaaccat
    24961 gtcttgatcg cctgcgccgc cgccgatgcg cgtcatgagc taaaaatatt tagctatctg
    25021 gcaagcgtat tgtgtcagca tccggcagaa gttattgccg ggttaacagg atatgaggca
    25081 tttatggagt tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat
    25141 ttatcatcaa aaacgtttaa cgcaggctat tatagtcatc aaccatttcg ttgtactcgc
    25201 gtaacgctcg tggaaatgag tcttcgacca tccagcttgt gtttgcatcc tgaagatgct
    25261 ctttatccca gtcagagtac gggactttat ctctgacgcg gcgaacatat tttttagtct
    25321 cgagttgata ctggtctgcc gaattaatga acgagaaatt cataccgcct ttatccgctt
    25381 ctttggcctg cgctaccagc gtttccagtt cagagacttt cttcattgcc gcatcaacat
    25441 caaacttctc ttgcgaaatc aggttattaa tttgtttcgc tgaaatcatg actgccagag
    25501 agtagtagtg gaatgttttc ccttctcttt cttcaatatt tttcagttcc gcaagctggc
    25561 gcttatcatt aatttcctga atcgccgcat gataagattc tgctacaggt tcaaaggatt
    25621 ccagattttt taagaatgtc tggtgcagcg ttttcccttt cgcaaacgca tcgtctttat
    25681 aattttcctg ggtgtaatat ttatccattt cgttaatggt attacccaac gccacggcag
    25741 catcaatata actcaccgcc acgccatcaa taggttgcag cgcaggcgtt aacgccacag
    25801 cactttttac gcctttttca cactcagcga ggttggattc actaatgccg tagatgccat
    25861 aaacagtact ctctttaccg gtaggcccct gtttaaaatc tttcagccag tcagcataac
    25921 gcgccagact gttctgtaca gggatctgta acttgttgta gcacttgata tagacatcca
    25981 ttttttcgtc gaaaaccgct tgttcatcga ccaccggttg cgcaggtgtt tcaggtttag
    26041 cttctgcttt tgcaacggga gcttcaggag ctggtgcggc aggttgacta ttggcaggcg
    26101 ggggcgtttc tgtttcggct tttttatcat cacaacccgt cagaccgagg gccatgatgg
    26161 cgactataat agcggaagat aataaattcc ttttcattta attgccttat aaatatatag
    26221 ataagagagt gaaca
//
LOCUS       DAZPJP010000042        25639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPJP010000042 DAZPJP010000000
VERSION     DAZPJP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.042"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(67..798)
                     /gene="rlmB"
                     /locus_tag="ACHU9J_001596"
     CDS             complement(67..798)
                     /gene="rlmB"
                     /locus_tag="ACHU9J_001596"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1905171.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(978..3419)
                     /gene="rnr"
                     /locus_tag="ACHU9J_001597"
     CDS             complement(978..3419)
                     /gene="rnr"
                     /locus_tag="ACHU9J_001597"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1905172.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3458..3883)
                     /gene="nsrR"
                     /locus_tag="ACHU9J_001598"
     CDS             complement(3458..3883)
                     /gene="nsrR"
                     /locus_tag="ACHU9J_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1905173.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4088..5386)
                     /gene="purA"
                     /locus_tag="ACHU9J_001599"
     CDS             complement(4088..5386)
                     /gene="purA"
                     /locus_tag="ACHU9J_001599"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1905174.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEIKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5490..5687)
                     /locus_tag="ACHU9J_001600"
     CDS             complement(5490..5687)
                     /locus_tag="ACHU9J_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1905175.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5769..6773)
                     /gene="hflC"
                     /locus_tag="ACHU9J_001601"
     CDS             complement(5769..6773)
                     /gene="hflC"
                     /locus_tag="ACHU9J_001601"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1905176.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6776..8035)
                     /gene="hflK"
                     /locus_tag="ACHU9J_001602"
     CDS             complement(6776..8035)
                     /gene="hflK"
                     /locus_tag="ACHU9J_001602"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1905177.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8121..9401)
                     /gene="hflX"
                     /locus_tag="ACHU9J_001603"
     CDS             complement(8121..9401)
                     /gene="hflX"
                     /locus_tag="ACHU9J_001603"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1905178.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9478..9786)
                     /gene="hfq"
                     /locus_tag="ACHU9J_001604"
     CDS             complement(9478..9786)
                     /gene="hfq"
                     /locus_tag="ACHU9J_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1905179.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9872..10822)
                     /gene="miaA"
                     /locus_tag="ACHU9J_001605"
     CDS             complement(9872..10822)
                     /gene="miaA"
                     /locus_tag="ACHU9J_001605"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1905180.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10815..12662)
                     /gene="mutL"
                     /locus_tag="ACHU9J_001606"
     CDS             complement(10815..12662)
                     /gene="mutL"
                     /locus_tag="ACHU9J_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1905181.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12672..14009)
                     /gene="amiB"
                     /locus_tag="ACHU9J_001607"
     CDS             complement(12672..14009)
                     /gene="amiB"
                     /locus_tag="ACHU9J_001607"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1905182.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSLRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14028..14489)
                     /gene="tsaE"
                     /locus_tag="ACHU9J_001608"
     CDS             complement(14028..14489)
                     /gene="tsaE"
                     /locus_tag="ACHU9J_001608"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1905183.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14461..16008)
                     /gene="nnr"
                     /locus_tag="ACHU9J_001609"
     CDS             complement(14461..16008)
                     /gene="nnr"
                     /locus_tag="ACHU9J_001609"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1905184.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16007..17146
                     /gene="queG"
                     /locus_tag="ACHU9J_001610"
     CDS             16007..17146
                     /gene="queG"
                     /locus_tag="ACHU9J_001610"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1905185.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17129..17182)
                     /locus_tag="ACHU9J_001611"
     CDS             complement(17129..17182)
                     /locus_tag="ACHU9J_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905186.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17416..17491)
                     /locus_tag="ACHU9J_001612"
     tRNA            complement(17416..17491)
                     /locus_tag="ACHU9J_001612"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Gly,seq:gcc)
     gene            complement(17527..17602)
                     /locus_tag="ACHU9J_001613"
     tRNA            complement(17527..17602)
                     /locus_tag="ACHU9J_001613"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17567..17569),aa:Gly,seq:gcc)
     gene            complement(17760..17835)
                     /locus_tag="ACHU9J_001614"
     tRNA            complement(17760..17835)
                     /locus_tag="ACHU9J_001614"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17800..17802),aa:Gly,seq:gcc)
     gene            complement(18046..18591)
                     /gene="orn"
                     /locus_tag="ACHU9J_001615"
     CDS             complement(18046..18591)
                     /gene="orn"
                     /locus_tag="ACHU9J_001615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1905187.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18686..19738
                     /gene="rsgA"
                     /locus_tag="ACHU9J_001616"
     CDS             18686..19738
                     /gene="rsgA"
                     /locus_tag="ACHU9J_001616"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1905188.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19835..20803
                     /gene="asd"
                     /locus_tag="ACHU9J_001617"
     CDS             19835..20803
                     /gene="asd"
                     /locus_tag="ACHU9J_001617"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1905189.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20825..24148
                     /gene="mscM"
                     /locus_tag="ACHU9J_001618"
     CDS             20825..24148
                     /gene="mscM"
                     /locus_tag="ACHU9J_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1905190.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24299..>25639)
                     /gene="yjeM"
                     /locus_tag="ACHU9J_001619"
     CDS             complement(24299..>25639)
                     /gene="yjeM"
                     /locus_tag="ACHU9J_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1905191.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5930 a         7192 c         6501 g         6016 t
ORIGIN      
        1 attatcttac cagctataga caaaaaaacc atccaaatct ggatggcttt tcataattca
       61 gacgaactag ctgcgctgac gaaccgcttc aaataagcaa attccggttg caaccgaaac
      121 gttcagggaa gaaacacttc ctgccattgg gatgctgatc aactcatcgc aatgttcacg
      181 ggtcaggcga cgcatacctt caccttccgc gcccatcacc agcgccaagc gtccggtcat
      241 tttgctctga tagagtgtat gatccgcctc gcctgccgta ccgacgatcc agatattctc
      301 ttcctgcaac atacgcatgg tgcgcgcaag gttagtcacc cgaatcagtg gaacgctttc
      361 tgccgcgccg caggctactt ttttcgccgt agcgttgagc tgtgcggagc gatctttcgg
      421 cacaatcacc gcgtgaacgc cagcagcgtc cgcgctacgc aggcacgcgc cgaggttgtg
      481 cggatcggtt acaccgtcga ggatcagcag gaacggttga tcgagcgaag cgatcagatc
      541 cggcagatcg ttttcctgat actgacgtcc tggcttcacg cgggcgataa tgccctgatg
      601 cacggcaccg tcgcttttct cgtcgagata ttggcggttt gccaactgga taaccacgcc
      661 ctgagactca agggcgtgaa tcagcggtaa cagacgttta tcttcacggc cttttaaaat
      721 aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg gcctgcactg cgtggatgcc
      781 gtaaatcatt tcgctcatta atgtactcgt tgtttacgtt ttgcctgatg cactacgttt
      841 atccggccta catgatctct gcaatatatt gaatttgcgt gattatgtag gccggataag
      901 gcgttcacgc cgcatccggc atgaacaaag cgcattttgt cagcaatcta accctcttct
      961 tttaaagagg gtattgatca ctctgccact tttttcttcg ccgcacgctt cgctttggtc
     1021 gctgcggcta ttttctgcgt tttcgccgat ggctttttcg cttttctcgc gtctttcttc
     1081 gccgctttcg gcttcgtttt tttctcaccg cggaaggcgc tgtctggctc aaagtttacc
     1141 tttttaccga cctgacgacg cttgccgcct tttttgcctg catcgccttt tttcgctttc
     1201 tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcac tggagatcag actaaagtcg
     1261 attttgcgct cgtccatatt aaccgcttcg acgcgaactt ccacgcgatc gcccaggcga
     1321 taagtctggc cgctggattc ccccatcagg cgttgcccta cctggtcaaa gcgatagtag
     1381 tcattgtcca gcgaagagac atggaccaga ccatcaatga acaagtcgtc cagacggacg
     1441 aagaagccaa agccagtgac gctggaaatt acgcctttaa agacgttacc tacctggtcg
     1501 agcatgaagt cacacttcag ccagtcagcg acatcgcgcg ttgcttcgtc ggcacgacgt
     1561 tccgccatcg aacagtgctg acccagttgc aacatctctt ccatcgaata atggtagccg
     1621 ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg ccagcagata cttaatggcg
     1681 cggtgcagcg tcaggtcagg ataacgacga atcggcgaag taaagtgcgc ataggactgc
     1741 aatgccaggc caaagtgacc acggttttct ggatcgtaaa tcgcctgttt catcgagcgc
     1801 agcagcatgg tttgcagcat ttctgcatca ggacggtcgg caaccgactc cagcagttcc
     1861 gcgtagtcac gcggttccgg cttgttacca cccggcagct ccagccccag ctccgccagc
     1921 actgaacgga aagaggtaat cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc
     1981 ggttctttcg ctttctcaac gaaacgcgcc gccgagatat tcgccagaat catgcactct
     2041 tcaattaatt tgtgcgcgtc gttacgctgg gtctgttcga tacgttcaat acggcgttca
     2101 gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga tcccaccgcg ttcttcacgg
     2161 gctttatcca gcactttata gaggttatgc aactcttcga gatgcttaac cagcggggcg
     2221 tactgctcgc gcagatcctg atcgccctgc agaatatgcc agactttggt gtaggtcaga
     2281 cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc ccgtcaggcg gcctttcgac
     2341 gaaaccgtca tctcgcacac catacacagg cggtctacct gcgggttgag cgaacacagg
     2401 ccgttagaga gcacttccgg cagcatcggg ataacctgcg aagggaagta caccgacgtg
     2461 ccacggttac gcgcttctct gtccagcggc gttggcggac gcacatagta gctgacgtcg
     2521 gcaatcgcga cccataaacg ccagccgccg ccgcgttttt tctcgcagta aactgcatcg
     2581 tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg gtaaatcgcg caaatcgaca
     2641 cggcccgctt ttgcttcttc cggcacttct tctttcagcc ccgcaacctg ttgctcaaca
     2701 gcctgcggcc agatatacgg aatttcatgg gtacgcagag cgatatcaac cgccatgccg
     2761 gtgcccatat tgtcgcccag cacttcgacg attttaccca ccgctttggt gcggcgagtc
     2821 ggacgctgag tcagttcgac tacgaccaca aagcccatcc gcgcgcccat gatctgatcg
     2881 ggcgggatta agatatcgaa gctcagacgg ctatcgtcag gaaccacaaa gccgacgccc
     2941 gcttcggtaa agtagcgacc aacaatctgg ctggtttttg gcaccagtac gcggacaata
     3001 cgcgcttcac gacgaccttt acggtcagca cccagcggct gcgccagcac ctgatcgcca
     3061 tgaatacagg ttttcatctg ctcgctggag agatacaaat catctttacg cccttcaacc
     3121 cgcagaaagc cgtagccatc acggtggcca ataacggtac ctttcaccag gtcgaggcgt
     3181 tccggcagcg catagcactg acgacgagtg aagaccagtt gaccatcgcg ctccatcgcg
     3241 cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc
     3301 agctcatcac ggctggccgg tttttcacgt ttggttaaat gttcgaggat aaattcccga
     3361 ctagggatgg gattcgcgta tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc
     3421 tcggttcctc cgttgtcatc tctgatgaag attttcgtca ctccaccagc aataatttat
     3481 aaagcggttg attctcttca accaaatcgg caagcgtgta gttatccagt tccgtaagaa
     3541 aactttgcac ggccttagaa agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa
     3601 actcactgct gcaattcacc agcgataagg gctccagctc gcgcaccaca tcaccaatac
     3661 gtatcgcact cgccggttta cccaggcgaa tgccgccatt ttttccacga acagcagtca
     3721 cgtagccggc acgactaagt tgattgatta ttttgaccat atgattacgg gagacgccgt
     3781 agacgtcagt cacttcagaa atactggtca tccgcccttc tggcaatgac gccatgtaga
     3841 tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga
     3901 aatcgggaaa aaatacattt atattgatga taaaccagcc acaagctgtg tcgctaattt
     3961 atttcagttc ggggaaggaa aaaagcgagg atttaacact gtgtcggata gcgtaatggc
     4021 ttaaccgaca cagcaatgta tcacactgtt agcccggcag gcaaaatatc tgccaggcgt
     4081 accagaatta cgcgtcgaac ggatcgcgca gaatcatggt ttcagtacga tccggaccgg
     4141 tagagatgat atcgatcggc acaccggtca gctcttcaat acgcttgatg tagttcagcg
     4201 ccgcctgcgg caggccgcta cgatctttca cgccgaaagt ggattcagac cagcccggca
     4261 tggtttcgta aatcggctct acacctttcc agtcgtcagc tgccagcgga gtggtagcca
     4321 cttcgcgacc atccggcata cggtaagcca cgcagagttt aacctctttc aggccatcca
     4381 gaacgtccag cttggtcagg cagaagccag acagggagtt cagctgtacc gcacgacgaa
     4441 cggcaacggt gtccagccag ccggtacgac gacgacgacc cgtagttgcg ccgaattcgt
     4501 taccctgctt gcagaggaac tcgccagttt catcaaacag ttcggtcggg aacggacctg
     4561 cacctacacg agttgagtaa gctttgagga tacccagaac ataatcaaca taacgcgggc
     4621 ccaggccgga accggtcgcc acgccaccag cagtggtgtt ggaagaagtt acgtacggat
     4681 aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc ttcaaacatg acgaaatcgc
     4741 cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac caccatagaa gtcaggatgt
     4801 cggcaacagc catcgtatca tccagaactt tctggtaatc aaccgcttca gctttgtagt
     4861 agttaaccaa ctggaagttg tgatattcca tcacttcttt cagtttttca gcgaaggttt
     4921 ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc tactttatct tcataagcag
     4981 gcccgatacc acgaccggtg gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt
     5041 tatccagcgc aacgtgataa tcaaggatca gcggacatgc ttcagacagc agcagacgct
     5101 cacgaacggg gatgccacgg tcttccagtt cttttatctc tttcatcagc gcggccggag
     5161 acagcacaac accgttaccg atgatgctgg ttacattctc gcggagaata cctgatggaa
     5221 taagatggag aacggttttt tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc
     5281 cctggtagcg tacaacatat ttagcccgtt cagtcagaag atcgacgatc ttacctttac
     5341 cttcgtcacc ccattgggtg cccagtacga cgacgttgtt acccattttt caaaatcacc
     5401 gtttgcttaa aaatggattc taccatcgct ttttcagagt tacagcactt tttgcactca
     5461 aaaatgacct cgatcagtct gttttttgtt cagccaatcg ttttcctcaa catgtagtag
     5521 accacaacgc ccgcaaccac aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa
     5581 ttggtcatcg cagagatcat cttcttccat gccttcgggt aaagcatcgg ccctaaacct
     5641 tccagtacca aaaccagggc aagcgccagc cagattgtcg aattcattca ttatccttat
     5701 agaaaaagaa aaccaccgac atccctgagg atgcggtggc tttattgacc tgtaccgcag
     5761 tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc
     5821 gggctcatga ccatcacgtc ctgattgcca gagaagctgt tctcataagc acgcaggcta
     5881 cggatgaatg cgtagaagtc cggatcttta ctgaatgcat cagcaaacag tttggctgct
     5941 tcggcatcgc cttcaccacg catgatgcgg ccctgacgct cagcttctgc cagcgttctg
     6001 gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg
     6061 tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt tgtagatcgc ttcagacact
     6121 tcggtcggca ggttgatctg cttgatacgc acatcgacaa cttcaatacc cagcgccgcc
     6181 atactgttcg ggttgatgac cggaactttg cccttcgtct cagccgttac gcgctctgcc
     6241 gcttcggcaa tggcgttatc tgccgccggg gtagtaactt catcttctgt acccgcagaa
     6301 ccggagttca gcgcgtcacg tacttcgagg gtcagacgac cacgggaatc ggtgacgatg
     6361 tctttcacgt ccaggcgacc aatttcagaa cgcagacggt cagagaactt acgtttcaac
     6421 agcacttccg cttgcgaaat gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg
     6481 ctgatgcgcc atttgatgta agaatcgacg atcaggtctt tcttctcttt ggtcacaaag
     6541 cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg
     6601 aacggtatct tgaaatgcag acccggctca taaaccagag gtttgttgtc atcgtcacgc
     6661 agtaccttac caaaacgcag cgtaataccg cgctcacctt ctttgacgac aaagacagac
     6721 atgtaaagca ctaccagcac gatgatgata atcgcgataa ctgacttacg catcgttatt
     6781 ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc
     6841 cctgactggt ggacgacgtg ttgcttgctc cactggttgt ggaggaagag gctggcggca
     6901 gacgcagcag attgctggca ccgttatcgc tcttcgccgc aggggcgtta ccgcctttca
     6961 gcatctggtc taacggcaga accatcaggt tgccaccttt atcgttaacc agcactttgc
     7021 gggtgttacc caacactttt tccatcgtct cgatatacag acgctcgcga gtaatttccg
     7081 gcgcggcttt atattccggc agaagtttag caaagcgcgc cacttcaccc tgagcttcca
     7141 ggatggtctg ggccttgtac gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg
     7201 cacgcggctg aacttcgttg gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt
     7261 cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc ttccggcgga cgagctgcct
     7321 ggaagttgac gtccagcaga gtgataccca tgtcatacgg acggatagtc tcttccagtt
     7381 cgcgctgagt atcgctacga atcacggtac gaccttccgt cagaatgcgg tccatggtgt
     7441 atttaccgat aactccacgc agggcgctgt cggtagcctg acgcaggctg tcatccgggc
     7501 tggtcacgct atacagatat ttttccggat tggtgacgcg gtactgcacg ttcatctcaa
     7561 cgcgcactac gttctcgtcc gacgtcagca tcacaccaga agcggccagt tcacgcacgg
     7621 cttccacgtt caccggtttg acttcgtcga taaacgtcgg tttccagttc agacccggct
     7681 caaccagatg gctgaattta ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg
     7741 tatagaaacc actggccgcc cagataatga caatcgctgc cgctgcgatg gtaacgacac
     7801 gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc accgccagat ccggtgcctt
     7861 taccgccgcc cagaccaccg agctttttgc tcagtttgcg gaagatatca tctaaatcag
     7921 gtggcccttg atcgcgaccg cctttgtttc catttccctc agagttgccg ccaggtttgc
     7981 tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg ctgattccac gccatgtttg
     8041 tgctccatat ttgttatgcg gtgatccctg ttgtctttcg acgccagagg atatgactcc
     8101 acgcttcaga cgctacgccg ttagatcagg taatcgatca acgccggttc ttgtttacag
     8161 aggcgacgcc agtcaacgat cggcatacga acttgcagac ttacgctgcc gtcctcctcc
     8221 atccactctt tttctattgc ctgaagctga taaaaacgac ttctcagacg tccttcctgc
     8281 ggcggcaaac gcaatgtatg ctgcgccacc tcgccggaaa gccgctccgt caaagcctga
     8341 aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc agacacggat cggtttgttc
     8401 tcttcgtccc gatcaatacg cggttcgaaa tcttccagca tatcgatctt gttcatcacc
     8461 agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg
     8521 ttttcttgta cacgtacatc cgccgcatca atgacgtgca gcagtaatgt ggcttgccgc
     8581 gtctcttgta acgtggcttt aaatgctgcc accagatcgt gcggcaggtg gcgaataaac
     8641 cctacggtat ctgcaagtac ggtttcaccg acatccgcga cgtcaatacg ccgcaacgtc
     8701 gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg
     8761 aaaagggtag atttaccggc gttggtatat cccaccagcg aaacagtagg aacgtcggct
     8821 ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa ctctttctag gcgcgactgt
     8881 atctgcacga tgcgattacg caacaaacga cggtcggttt cgagctgggt ttcacccgga
     8941 ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg tccagccacg caccaggcgc
     9001 gtagccagat ggcgcagctg cgccagctca acctgcaact taccctcatg ggtacgcgca
     9061 cgttgggcga aaatatctaa aataaggccg gtgcggtcga taacacgaca ctcgcacaaa
     9121 cgctccaggt tacgctcttg cgccgggctc agggcatggt caaaaagaac gaccgaagca
     9181 cccgtcgctt tgacagcttc cgcaatttca actgctttac cttcacctac aaaatacttt
     9241 gggtgcggcg ctttacggct accggtaatc acctgcaatg cttcgacacc ggcggaagag
     9301 accagagatt caaactcctg gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt
     9361 accagtaccg cctgctcacc agcatcataa cggtcaaaca agcgtataac cctctaaata
     9421 gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa aaaaacagcc cgaaacctta
     9481 ttcggtttct tcgctgtcct gttgcgcgga agtattctgc gcgctgctac catgatggta
     9541 gttactgctg gtaccgccac cggcgttgtt actgtgatga gaaaccgggc gagacgggac
     9601 aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc gtgtttttca acaggatcac
     9661 gaactgatca aaagactcga tttgcccttg cagcttaata ccattcacca aataaataga
     9721 aactggaaca cgttcccgac gcagtgcgtt caggaacgga tcttgtaaag attgcccctt
     9781 agccattctc tcttttcctt atatgcttat ttgtactttg aacctttcga ttctgaaaaa
     9841 attgcgcacg atacgtctca attgtacaca ttcagcctgc gatagcacca acaacctgta
     9901 atacttcgtc acgcgcctgt tctggttttt cactgtcaag ccagtgaacc ccttcccaac
     9961 cacgcagcca ggttatctgc cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa
    10021 ccatttcatc gtatgagatt tcgccttcaa ggtaagacca catctggcga taacccacgc
    10081 aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg
    10141 cttcaaaacc tgaagccaac atctgatgaa aacgctgctc aatgcgttga tggagcagtt
    10201 cacggctcgc cggggcgatg gcgaactgat gcacctgata cggtagagcg tctcctgacg
    10261 tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa aacttccagt gcccgggaaa
    10321 gcctttgtgg atcatttgga tgaatccttg ctgccgcaac cggatctacc tcctgaagtt
    10381 gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg ctcaattctg gcccgcactt
    10441 ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg
    10501 taccgcccac taacagtggg atccgccccg ccgcggtgat atcggccatt tccgccagcg
    10561 catcgcggcg aaaatcagca gccgagtaag cctgcgacgg atcgcgaata tccagcaatc
    10621 ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc cgtcccgata tccatccctt
    10681 tgtaaataag ggcagaatca acgcttatca actctactgg taaaatttta cgcagctcaa
    10741 tggctaacgc cgttttaccg gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc
    10801 tcgccttact gatatcactc atctttcagg gcttttatcg ccggatgtaa atcaacagat
    10861 tgtaacagac cacccggcgg cgttttcaca agttgcggac ataaccgttc cacgtccgcc
    10921 agcagggtta tggcctgtgc cattgaccac tgcgcatgtt cgctcatcag atttcgtgca
    10981 atccactgcg caatattgcc aggttcgaat acggactgct tcgccaggta gcctatcagt
    11041 tcaggaatca agatttgtaa attttgttgg cgtaagggta aaggcacggc cctgatggtc
    11101 acatgctgtg catctgactg gaaatcaata cccaattccg ccagggcaga ctgcgctttt
    11161 tctaatgccg atttttcttc gccagaaact tttagccgca acggaataag cagcggctga
    11221 gcgcaaactg gcgcttcacc cggcgtcagt tgtacctgac gcagccaacg ttctgccact
    11281 ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca acgcacagtc ggaatggacg
    11341 atagtcagta cccgaccaaa actctgactg ttcgccgcaa gtgcaggttc ctgcggttcc
    11401 ggcgctttta atttttgcat cggcgcgggc gtttgcaaaa gctggcgata cacttcacct
    11461 tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg cagccggacg actgcctgat
    11521 gctggcgcag gagtgtagcg cggagctacc ggctcacgag ctgccggttc tgcaaagtga
    11581 ttgcgccccg ccgccacgcg gttttccgga atggaacgcg gtgcaggttg gggttcatcg
    11641 tccagcggta gcggcgtttc cagttgctgt tgtagcacgc tcagcacgcc ctgatagata
    11701 aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt gtttggcggg gtgcacgttg
    11761 acgtccacct gatgcggatc gatctccaga tacagcacaa atgccggttg ctgatcggcc
    11821 cccagtttgt cttcgcaggc ctggcggatc gcgtgattga tcaggcgatc gcgcatcatg
    11881 cgaccgttca cgtagcaata ctgaatttct gccagtgcgg gcgtggtgtg atttggatcg
    11941 gccacccagc cgcgtagcgt gagatcgccg tgttgccatt caatcgccag cgcttgttca
    12001 agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt tttgcccgcc ttccggcact
    12061 gcgcggtact gacgcacaat tttaccgtta tgcgacaggt tgatcgtgac gtcgaaacgc
    12121 gccagcgcaa tgcggcggat gatctcatca atgtggttaa attcggtttt ctcggtgcgc
    12181 aggaatttgc gccgcgccgg ggtgttgtag aacagatcca gcacctccag cgtcgtcccc
    12241 acaggatgcg ccgccggttt taccgtcacg ttcatatcgc gcccttcggc ataggcctgc
    12301 caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata
    12361 ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg cttcgagatc gtccagagag
    12421 gcgattttac tggtggcatg acgagccagc gccagcgcca gctcatcttt tttgataccg
    12481 cagccgttat cacgaatgcg gataagtttc gccccaccgc gttcgatatc aatatcgata
    12541 cgcgtcgcac ctgcatcgag gctgttttcc accagttctt tgactaccga cgcaggtcgc
    12601 tcgaccacct cacctgcggc aatctggttc gccagttgtg gcggtaagac ctgaattggc
    12661 atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg tcaccgtact ggcagtttgt
    12721 gccgttgccc cctgcggcgc agattgcatc ggatgcgcaa ggaaataatt gcgcaggcct
    12781 ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg
    12841 ctgttgttgc tgataaaacc ggtttcgacc agtactgacg ggatatccgg cgaacgcaga
    12901 acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt cgccaatgcg ttgcaactga
    12961 ctgatcatac tggtcgctac atcataccct acccgcaggg aatgaccgaa ctgtaaatcc
    13021 agcaccgcct ggcttaaata ggggtcagac tgactgttcg ccagcacatc gcccgcccca
    13081 cccagcagct ccgactgttt ctcgtgctgc tccagccagc tggccatttc gctgttggcg
    13141 cgacggttag agagcaccca tacggaagcg ccagtcgcac tgcggttcgg tgcggcatca
    13201 gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg ccacatcgct gcgccccatc
    13261 accgagataa agtaatcccc gtcacgggtt aaaacgcctt taaacatcgg atcgtcattg
    13321 agcaaagtgc gcaatttacg cgcgatggcg atggtgacat ttttctcccg cgtaccaccg
    13381 gggccgatag cgccagggtc ctgaccgccg tgtccggcat caatagcgat gataatttta
    13441 tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta ccgtattact gctgataaca
    13501 cccgtagtgc ggttactttc cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt
    13561 gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg gcggtggagg aggcggtggc
    13621 ggcacatcgg cgttaatcgt aaagacgaca gtgtaattgc tgccattctg ccgcttcacc
    13681 gcttcggttt taccgttttc ggtaagatcg accaccagcc gtagcgtttg tgcatcttta
    13741 ggcgttccag agcgaatcgc cttcaccaga ttattgccgc tgaacaacaa cggcagtccc
    13801 tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc gtttgctttg atggctaaac
    13861 gcataatcag gatcgccaat aaaactcaac gttatccgcg cctgttgatt accgttagaa
    13921 acctgaatat cagagagcgt cgcggcaccc accggcgtgc acagcagcag cagcgtcgct
    13981 accaaccaat ttctgatgcg atacatcatc ccgccacctt tcaaaggtta accggctaaa
    14041 cgcgccagca acaattcacc cgcagaggaa accgcactca cgcgcgcctc acggccttgt
    14101 gcctgataat caatgtgtat ttcgacatcc gggtcaggaa gaacacctgt accttgttgt
    14161 ggccactcca ccaggcagat ggcatcgttg gcaaaataat cgcggatccc cataaactcc
    14221 agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc
    14281 gtatagggtt cgaccagcgt ataagtgggg cttttgacat taccctgatg acccagagct
    14341 tgtaaaaaac cccggctaaa ggtggtttta cctgcgccta aatcgccata cagatagatt
    14401 acggttgcgc catcgcaggc tttcgctacc cgctcgccca ggtctaatgt tgcctgctca
    14461 tcagggagcg gaattactcg attcatcatg gtttttatca gtcacttccg ggttaacaat
    14521 acgctgtagc gtggaaaaga gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc
    14581 cagtacgtca gctgccgcac cgtgcgcgac acagcccgca caggcggcat catacggcga
    14641 cagtttttgc ccaagcaatg cgccaataat accagagagc acatcgccca tgccgccgct
    14701 cgccatgcct gcatttccgg catcaataat gcctaaagcg tcaggatggg cggcgaccac
    14761 ggttccggca cctttcagca ccgctacgcc gccataacgt tgtaccagac gtttggcgca
    14821 atgtaagcgg tcactttcaa tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc
    14881 aggatgcggc gtgatcacgc gattgtgacg cttatcggga ttgattgcca gcaggttcaa
    14941 tgcatcggca tcccacaaca tcggtttgcg aaaattctca actttttgca gtgctttttt
    15001 cccccactct tgctggccca gaccgggacc aatcaccacc acatcggccc attccaggct
    15061 ttcggtaaga gagtccatcg tcagttcatg caccatcaat tccggtcgtg cagtcagcag
    15121 cggcgcaatg ttttcactgc gggtcagtac tcggactaaa ccagcaccag cacgcagcgc
    15181 cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga tcgccaccga taatcaccag
    15241 ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt ttcagccagt gagaaagttg
    15301 ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc agccagctat ccagccccag
    15361 tgagtcaaaa tgcagttgtc ctgtaacatc ccgcgctttt ccagtgagca agcctggttt
    15421 cagcgcaata aaagtgatgg tgtgatcggc gttgatcact gcgcctggcg tagcgccggt
    15481 ttcagccagc aggccggaag ggatatcaac cgccacaatc ggcgcaggat gggaattagc
    15541 gtggtcgatt aactggctaa tggattcgcg gggcgcttgc tgcaagccgg taccgagcag
    15601 cgcatcaaca atcagatcta ccgattcggg ccagacaata ttcgaagcat ggatctcgcc
    15661 acccgcgttt aaccatgctt cgcgtgccag cgcggcctct tccggcaacg gtttgtcgct
    15721 ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg gccagtcgcg cgaccacgta
    15781 gccatcgccg ccgttattac catgaccgca cagcaccagc cagtggcggg cgtcaggata
    15841 cgccgaacga cacacctgga atgcggcctc gccagcgcga agcatcagct catagagtgt
    15901 gagccccagc acatctgccg cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac
    15961 ggtgtgtggt atacttacgg ggtttttctt cattgtatgg tccgtcatgt cagagcccct
    16021 cgatctcaat cagttagcgc aaaaaattaa acagtggggg ctggaactgg gctttcagca
    16081 ggtaggtatt accgataccg atctcagcga gtccgagccc aaactgcaag catggctgga
    16141 caaacaatac cacggcgaaa tggactggat ggcacgtcac ggtatgctgc gcgctcgccc
    16201 ccatgagtta ttaccaggta cgctgcgtgt aatcagcgtg cggatgaatt accttcctgc
    16261 taacgccgca tttgccagca cgctgaaaaa ccccaaactc ggctatgtta gccgttatgc
    16321 gctgggccgt gactatcaca aacttctgcg caaccgactc aaaaagctgg gcgagatgat
    16381 tcagcaacat tgtgtttcgc tgaattttag accgtttgtc gattctgcgc ctattctcga
    16441 gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt aagcactcac ttatcctcaa
    16501 tcgcgaggcc ggttctttct tctttttagg cgaattgctg gtcgatattc cgctgcccgt
    16561 ggatcaacca gtcgaggaag gatgcggtaa atgcatagcc tgtatgacga tttgcccgac
    16621 cggtgccatc gtcgagccat ataccgtcga tgctcgccgc tgtatctctt atctcaccat
    16681 cgaacttgaa ggggcgatcc cggaagagtt gcgaccgtta atgggaaacc gtatttacgg
    16741 ttgcgatgac tgccagctta tctgcccgtg gaatcgctat tcgcaactca ctacagaaga
    16801 cgatttcagc ccgcgtaagc cgctacacgc acaggaactc attgagttat ttgcctggag
    16861 cgaagagaag tttttaaaag tcacggaagg atcggcgatt cgccgtatag gtcacctgcg
    16921 ctggctgcgt aatatcgccg tggcattagg caatgcgccc tgggatgaaa cgattctggc
    16981 ggcgctggag agtcgcaaag gtgagcaccc acttcttgat gagcacatag cgtgggcgat
    17041 tgcgcagcaa atcgagagac gaaatgcgtg catagtcgaa gtgcaattgc cgaaaaaaca
    17101 gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac gcctgattca tccacagctt
    17161 gtgaataaaa ataaaaacgc attgcaattc aagacgcata aaatcgtcaa gtgatcgact
    17221 cgacaaaaca gaatcttgat ttttaactta tttatcaata ggttaaaaaa ataccattca
    17281 ccgtgcgaag tttctttgaa attaagatta cagcctgagg atgtggataa ctctgtttac
    17341 aagagtttgt cagagacaac aaaaaccagc aataacgcat cgcgtcgctg tggatatttt
    17401 attgagagaa gaatttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17461 ggtcgtgctc taccaactga gctattcccg cttgggtggt ctgtgcctta cagcactttc
    17521 aaattttgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca aggtcgtgct
    17581 ctaccaactg agctattccc gcttaatctt cgtctttcga accgctactg ccttggctgc
    17641 cttcactcac cttagtcaca tacttttgta agttcccaag gattcgttca gttgccgcct
    17701 tgtcgcaatt cgaaatactg cgattgggtg gtctgtgctt tgcagcactt ttcaaatttt
    17761 ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
    17821 ctgagctatt cccgcgtact acttaatttt gctttcgtaa ttttcgcatt tctgcgtcgt
    17881 tacgggaggc gcattatacg agaaatcttt ccctctgcaa ccccccaaaa aagttttttt
    17941 tgcgttttta tctcaagtgc tgaaattatc agcaagacgt tcaatttagc gtcaaaatca
    18001 cgccgccgca agtggattcc ggcgcttacg tgaccaggaa aaattttaca gcttgataaa
    18061 atgctcgcgg tagtaagcca gctccgccac cgattcacgg atatcatcca ttgcctgatg
    18121 cgtcccctgc ttggtaaaac catccagaat ttccggcttc cagcggcgcg ccagctcttt
    18181 cagggtgctg acatcgagat aacggtagtg gaagtaggct tccagctccg gcatgtattt
    18241 aaacaggaaa cgacggtcct gtccgatgct gttaccgcaa atcggcgatt ttcccgcagg
    18301 cacccactgt tttaagaatt cgagcgttgc cagttcagct tcccgatcgc ccatcgtgct
    18361 cgctttcacg cgctctacca gcccgctggc ggtatgggtg cgcacgttcc agtcatccat
    18421 cagcgccagc tgttcatcgg actgatgtac tgcaatggtc ggcccttctg ccagaatatt
    18481 cagattggca tcggtcacca gcgtggcaat ctcaataatg cgatcgcgct cgggatccag
    18541 accggtcatc tcaagatcga tccaaatcag gttgttttca ttggcactca tgctattttc
    18601 cacccattca cagtgactat attgatccac aattgcgtgt atcatagatg ttttgcccat
    18661 caggggcgac caggagtcag tacgattgag taaaaataaa ctctccaaag gccagcagcg
    18721 ccgcgtgaac gccaatcacc agcgtcgtct taaaacgtct aaggagaagc ccgactacga
    18781 cgacaatctg tttggcgagc ctgatgaagg tatcgtcatc agccgctttg gtatgcacgc
    18841 tgatgtggaa tccgccgatg gcgacgttca ccgctgcaat attcgccgta ccatccgttc
    18901 gctggtaacc ggcgaccgcg tagtctggcg tccgggtaaa ccggcggcgg aaggcgtaaa
    18961 tgtcaaaggg atcgtggaag cggtgcatga gcgtacctcg gtgttgacgc gtccggattt
    19021 ctacgacggc gtaaaaccta ttgccgccaa catcgaccag attgtcattg tctccgccat
    19081 tttgccggag ctgtcgctca atattatcga ccgttacctg gtggcctgcg aaaccttgca
    19141 gattgagccg attattgtgc tcaacaagat agacctgctg gacgacgaag gcatggcgtt
    19201 cgtcaacgag cagatggata tctaccgcaa tatcggttat cgcgtattga tggtttccag
    19261 ccatactcag gatgggctaa aaccgctgga agaggcgttg accgggcgca tcagcatttt
    19321 tgccgggcag tctggcgtcg gcaaatccag cctgctgaac gcgttgctgg ggctgcaaaa
    19381 agagatcctg accaacgatg tctctgacaa ctcggggctt ggtcagcaca ccacgaccgc
    19441 cgctcggctg tatcacttcc cgcacggcgg tgatgtgatt gattcccctg gtgtgcgtga
    19501 gttcggcctc tggcacctgg agccggaaca aatcactcag ggctttgtcg aattccatga
    19561 ctatttaggt ctgtgtaaat atcgcgattg caaacacgat accgatccgg gctgcgctat
    19621 ccgggaagcg gttgaggaag ggaaaatcgc ggaaacccgt ttcgaaaact atcaccgtat
    19681 tctggaaagc atggcgcagg taaaaacgcg taaaaacttt tctgatacgg atgactgaca
    19741 attaagctaa ccgtcgttag aatcgtcccc tttttttcag gatccggcat gtaggccgaa
    19801 tcaggaacga caaaacaatg gcctggaggc taccttgtta aattcattta aactttcgct
    19861 acagtacatt ctgccgaaac tatggcttac tcgcctggcg ggttggggcg caagcaagcg
    19921 ggcaggatgg ttgacaaaac tggttatcga tctgttcgtt aaatactaca aggtcgacat
    19981 gaaagaggcg caaaagccgg acaccgccag ctaccgcacc tttaacgaat tctttgtccg
    20041 tccgctgcgt gacgaagtac gcccaatcga taccgatccg aatgtactgg tcatgcctgc
    20101 cgatggcgtt atcagccagt tgggtaaaat cgaagaagat aaaatcctgc aagccaaagg
    20161 ccacaactac agcctcgaag ccctgctggc aggcaactat ctgatggcgg acctgttccg
    20221 caacggtacg tttgtgacca cttacctctc cccgcgtgac taccaccgcg tacacatgcc
    20281 gtgcaacggt attctgcgtg agatgatcta cgtgccgggc gatctcttct ccgttaacca
    20341 tctcacggct cagaacgtgc cgaatctgtt tgcccgtaac gaacgcgtga tttgcctttt
    20401 cgataccgaa tttggcccaa tggcgcagat tctggtcgga gcgacgattg ttggcagcat
    20461 tgagacggtc tgggcgggca ccattacgcc gccgcgcgaa ggtatcatca agcgctggac
    20521 ctggcctgcc ggggaaaacg acggttctgt ggcactgctg aaaggccagg aaatgggtcg
    20581 ctttaaactc ggttccaccg ttatcaacct gtttgcaccg ggtaaagtga atctggttga
    20641 gcaactggaa agcctgtctg tcacgaaaat ttgccagccg ctggcagtat ctaccgaaac
    20701 ctttgttacg ccagacgctg aacctgcccc gcttcctgct gaagagatcg aagcagaaca
    20761 cgacgccagc ccattggttg acgacaaaaa agaccaggtc taatccatca aaggaaacgc
    20821 tgacgtgcgc ctgattatca cttttctgat ggcctggtgc ctcagttggg gggcgtacgc
    20881 cgcgacggcc cccgatagca aacaaatcac tcaggaactg gagcaggcaa aagcggcgaa
    20941 acccgcacag ccggaagtcg tagaggcgct tcagtctgcc ttaaatgcgc ttgaggaacg
    21001 aaaaggttcc cttgagcgca tcaaacaata tcagcaagtt atcgataatt atccgaaact
    21061 ctccgctact ctgcgcgcac aattaaacaa catgcgtgac aagccgcgca gcgtgtcgcc
    21121 gggaatgtct accgacgcgc tgaatcagga aattctccag gtcagcagcc agttgctgga
    21181 taaaagccgt caggcccagc aagagcagga gcgcgcccgc gagattgccg attcgctgaa
    21241 tcaactgccg caacagcaaa ccgacgcccg ccgtcagtta aatgagatcg agcgccgcct
    21301 gggaacgctt accggcaata ctccgctcaa tcaggcacaa aatttcgcgt tgcagtctga
    21361 ctctgcacgt cttaaggcgc tcgttgatga actggagctg gcgcagctgt ctgccaataa
    21421 ccgccaagaa ttagcgcgct tacgctcaga gcttgcggaa aaagagagcc agcaactgga
    21481 tgcgtatttg caggccttac gtaatcagtt gaatagccaa cgtcagcttg aggcggagcg
    21541 ggcgctggaa agtaccgaat tactggcaga aaacagtgcc gatttgccga aagatatcgt
    21601 cgcgcaattc aaaattaacc gcgaactatc ggcagcgctg aatcaacagg cgcagcggat
    21661 ggatctcgtt gcctcgcaac agcgtcaggc tgccagccag acgttacagg tccggcaggc
    21721 gttgaatacg ctgcgtgaac agtcgcaatg gctgggatcg tccaatctgc tcggtgaagc
    21781 gttgcgagcg caggtggcac ggctaccaga aatgccaaaa ccacaacagc ttgataccga
    21841 aatggcgcag ttgcgtgtgc aacggttacg ttatgaggat ctgcttaata aacagccgct
    21901 gctacggcaa atccatcagg ccgacggtca gccgctgacc gccgagcaaa accgtattct
    21961 ggaagcacaa ctgcgcactc agcgtgagtt gctgaactca ttgttgcagg gtggcgacac
    22021 gctactattg gaactgacca agctgaaagt ctccaacggg caactggagg atgcgctgaa
    22081 agaggtgaac gaagcaacgc accgctatct gttctggacc tctgacgtgc gcccgatgac
    22141 catcgcctgg ccgctggaaa tcgcccagga tctgcgtcgt ctcatttcgc tggacacctt
    22201 cagtcagttg ggcaaagcca gtgtgatgat gctgaccagc aaagagacaa ttttgccgct
    22261 gtttggcgcg ttgattctgg tcggttgcag tatttactcg cgccgctatt tcacccgttt
    22321 tcttgaacgt tcggcggcga aagttggcaa agtgactcag gatcacttct ggctgacgtt
    22381 gcgcactctt ttctggtcga ttctcgtcgc gtcaccgtta ccggtgctgt ggatgacgct
    22441 gggttacggc ttgcgcgagg cgtggcctta tccgctggcg gtcgcgattg gcgatggtgt
    22501 aacggccacc gtgccgctgc tgtgggtagt gatgatttgc gccacctttg cccgcccgaa
    22561 cggcttgttt atcgctcatt ttggctggcc gcgcgaacgc gtatctcgtg ggatgcgcta
    22621 ctacctgatg agcatcgggc ttattgtgcc gctgattatg gcgctgatga tgttcgataa
    22681 cctcgacgac cgtgaattct ccggttcgct gggaaggctt tgctttatcc tcatttgcgg
    22741 tgcgctggcg gtggtcaccc tcagcctgaa aaaggccggg attccgctgt atctcaacaa
    22801 agaaggcagc ggcgacaaca ttaccaacca tatgctgtgg aacatgatga ttggcgcgcc
    22861 gttggttgcc attctggcgt cggctgtggg ttatctggca acggcacagg cgctgttagc
    22921 gaggcttgaa acctcggttg ccatctggtt cctgctactg gtggtttatc acgttatccg
    22981 ccgctggatg ctgatccagc gccgcaggct ggcgtttgac cgggcgaagc atcgccgggc
    23041 agagatgtta gcgcaacgtg cgcgtggcga agaggaagcg catcatcaca gtagcccgga
    23101 aggagcaatt gaagtcgatg aaagcgaagt cgatctcgat gccatcagtg cgcaatcctt
    23161 gcggctggtg cgctcaattt tgatgttgat cgccctgctt tctgtcattg tgctgtggtc
    23221 agaaatccat tccgctttcg gcttcctcga aaatatttcg ctgtgggatg tcacctccac
    23281 ggtacagggc gtagaaagtc tggagccaat taccctcggt gcggtgctga ttgccattct
    23341 ggtgtttatc atcaccacgc agctggtgcg caacctgccc gcgctgctgg aactggcgat
    23401 tttgcagcac ctggatttaa cgccgggtac gggctacgcc atcaccacca tcaccaaata
    23461 tctgctgatg ctgattggcg ggctggtcgg cttctcgatg attggtattg agtggtcgaa
    23521 attgcagtgg ctggtcgccg cgctcggtgt tggtctcggt tttggtttgc aggaaatttt
    23581 cgccaacttt atctctggtc tgattatcct gttcgaaaaa ccgattcgca ttggcgatac
    23641 ggtgacaatt cgcgatctca ccggtagcgt gacgaaaatt aacacccgcg ccaccaccat
    23701 cagcgactgg gaccgtaaag agataatcgt gccgaacaag gcgtttatta ccgagcagtt
    23761 tatcaactgg tcgctctctg actcggtcac gcgcgtagtg ttgacgattc cggcccctgc
    23821 tgatgccaac agtgaagaag tgacggaaat cctgctcacc gcagcgcgtc gctgctcgct
    23881 ggtgatcgac aacccggcac cggaagtctt cctggtggat ctgcaacaag ggattcagat
    23941 tttcgagctg cgtatttatg ccgctgagat gggtcaccgt atgccgctac gccatgagat
    24001 ccaccagctg attctggctg gcttccatgc ccacggtatc gatatgccat tcccgccctt
    24061 ccagatgcgt ctggaaagcc tcaacggtaa acaaacgggg agaacgctga cgtctgcggg
    24121 caaaggtcgt caggcgggaa gtttgtaatg catgaagctg ccaaatgatg tcaacgtggc
    24181 tcatttagca gcttaacatc atcttcagtt atcaccggcc ctggagagta tcatccatac
    24241 gcattacttc ctgacaactc atcgccggag agacgatgag ttgcaggatt tcatatgatc
    24301 agtcgagagc taactcaggt ttattcgcca tgcgactgca atagttctgg taaatcgcca
    24361 tcgctaacag cgagaagaag acagggccgc caatcatcca caatgtgctg tcccagtctc
    24421 cggcttcaac cacaggctga ataatggtga agacgttcgc aaatgtcacc accagtacga
    24481 caaccaccat cgcaatcatt gccgacatac gcgttttgaa aatcacaaat ggtctgtcga
    24541 gatcctgacg tgctttaaag aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca
    24601 tagacacgtt cgccatcagc gtcagcttgt taaagaacgc ggatgcggta ccgccaccaa
    24661 acgaaaccag caggatgaag acagtaacca acccgcactg catccacatg gcgatagacg
    24721 gcatccccat cgcattcagg cgcgtcatcg gttccggcca caacgctttc ggcgtcccct
    24781 ggatgatggc tttcagcggt gaatagcaaa gcgtaaagaa cgcaccggta taagcgagga
    24841 acatcgaaag tccggtaata cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg
    24901 acaaatgcag tgcgttaccc agcgtcaccc cgaggctctt catcagcaca taggtaatat
    24961 tgccaaggtt aacggaacca ttacttaata cctgctgcca gtttgtgctg acgccccata
    25021 aaaatattgc cagcgaataa ccgattgaaa taacaatagc ggcaaaaaca atacctttgg
    25081 caaagttctt ttctggattt tccgttttat cgaccagacc accaaccgct tcaattccgc
    25141 cataggcaaa aatagcaaat accacaaacg atagcattgc cagaccggac tgataacccg
    25201 ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg cccaccattt aataacaaaa
    25261 tagtaatgct tactaacagc aatactaaat tcagacacat tactgcaata ccgcccaccg
    25321 cagtaatgcg ggcaatttta ttaatcccct ttgaagcaac gacggtgacc agaatcatcc
    25381 atgctactgc cagcagacca accacctgcg taggctccag tccggcaata cgccagtgct
    25441 gggtcatgtc gctaccatag aggaatgttg agaacggtac ccaaactttc gcggaggtac
    25501 tcaccatcca gatgatataa gaggaaaacc acataaacgt accaatgaag gcaaaacgtg
    25561 gtccgacact attattcatc caggaataga taccaccttc ttctttgcga taagcagctc
    25621 ccatttcagc catcattaa
//
LOCUS       DAZPJP010000043        25284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPJP010000043 DAZPJP010000000
VERSION     DAZPJP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.043"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHU9J_001620"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHU9J_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHU9J_001621"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHU9J_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1905192.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHU9J_001622"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHU9J_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1905193.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHU9J_001623"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHU9J_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1905194.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHU9J_001624"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHU9J_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1905195.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHU9J_001625"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHU9J_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1905196.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHU9J_001626"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHU9J_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1905197.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHU9J_001627"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHU9J_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1905198.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHU9J_001628"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHU9J_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1905199.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHU9J_001629"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHU9J_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1905200.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHU9J_001630"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHU9J_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1905201.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHU9J_001631"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHU9J_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1905202.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHU9J_001632"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHU9J_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1905203.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHU9J_001633"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHU9J_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1905204.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHU9J_001634"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHU9J_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1905205.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHU9J_001635"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHU9J_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1905206.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHU9J_001636"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHU9J_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1905207.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHU9J_001637"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHU9J_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1905208.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHU9J_001638"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHU9J_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1905209.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHU9J_001639"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHU9J_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1905210.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHU9J_001640"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHU9J_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1905211.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHU9J_001641"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHU9J_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1905212.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHU9J_001642"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHU9J_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1905213.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHU9J_001643"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHU9J_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1905214.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHU9J_001644"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHU9J_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1905215.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHU9J_001645"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHU9J_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1905216.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHU9J_001646"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHU9J_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1905217.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHU9J_001647"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHU9J_001647"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1905218.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHU9J_001648"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHU9J_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1905219.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHU9J_001649"
     CDS             14267..14635
                     /locus_tag="ACHU9J_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1905220.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHU9J_001650"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHU9J_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1905221.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHU9J_001651"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHU9J_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1905222.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHU9J_001652"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHU9J_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1905223.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHU9J_001653"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHU9J_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1905224.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHU9J_001654"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHU9J_001654"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1905225.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHU9J_001655"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHU9J_001655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1905226.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHU9J_001656"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHU9J_001656"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1905227.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHU9J_001657"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHU9J_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1905228.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHU9J_001658"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHU9J_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1905229.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHU9J_001659"
     CDS             21814..22356
                     /locus_tag="ACHU9J_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1905230.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHU9J_001660"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHU9J_001660"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1905231.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHU9J_001661"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHU9J_001661"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1905232.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHU9J_001662"
     CDS             23753..24010
                     /locus_tag="ACHU9J_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1905233.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHU9J_001663"
     CDS             complement(23986..24540)
                     /locus_tag="ACHU9J_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1905234.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="ACHU9J_001664"
     rRNA            25013..>25284
                     /locus_tag="ACHU9J_001664"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5993 c         6617 g         6232 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAZPJP010000044        25103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPJP010000044 DAZPJP010000000
VERSION     DAZPJP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.044"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            193..381
                     /gene="bcsR"
                     /locus_tag="ACHU9J_001665"
     CDS             193..381
                     /gene="bcsR"
                     /locus_tag="ACHU9J_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1905235.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            393..1145
                     /gene="bcsQ"
                     /locus_tag="ACHU9J_001666"
     CDS             393..1145
                     /gene="bcsQ"
                     /locus_tag="ACHU9J_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1905236.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1142..1306
                     /locus_tag="ACHU9J_001667"
     CDS             1142..1306
                     /locus_tag="ACHU9J_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR1905237.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1313..3760
                     /gene="bcsA"
                     /locus_tag="ACHU9J_001668"
                     /pseudo
     CDS             1313..3760
                     /gene="bcsA"
                     /locus_tag="ACHU9J_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3771..6110
                     /gene="bcsB"
                     /locus_tag="ACHU9J_001669"
     CDS             3771..6110
                     /gene="bcsB"
                     /locus_tag="ACHU9J_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1905238.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6117..7223
                     /gene="bcsZ"
                     /locus_tag="ACHU9J_001670"
     CDS             6117..7223
                     /gene="bcsZ"
                     /locus_tag="ACHU9J_001670"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1905239.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7205..10697
                     /gene="bcsC"
                     /locus_tag="ACHU9J_001671"
                     /pseudo
     CDS             7205..10697
                     /gene="bcsC"
                     /locus_tag="ACHU9J_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10779..12767
                     /gene="hmsP"
                     /locus_tag="ACHU9J_001672"
     CDS             10779..12767
                     /gene="hmsP"
                     /locus_tag="ACHU9J_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1905240.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12950..14236
                     /gene="dctA"
                     /locus_tag="ACHU9J_001673"
     CDS             12950..14236
                     /gene="dctA"
                     /locus_tag="ACHU9J_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1905241.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14456..15952
                     /locus_tag="ACHU9J_001674"
     CDS             14456..15952
                     /locus_tag="ACHU9J_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1905242.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="ACHU9J_001675"
     CDS             complement(16048..16977)
                     /gene="kdgK"
                     /locus_tag="ACHU9J_001675"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1905243.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17209..17976
                     /gene="pdeH"
                     /locus_tag="ACHU9J_001676"
     CDS             17209..17976
                     /gene="pdeH"
                     /locus_tag="ACHU9J_001676"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1905244.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18046..20106
                     /locus_tag="ACHU9J_001677"
     CDS             18046..20106
                     /locus_tag="ACHU9J_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1905245.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20288..21610)
                     /locus_tag="ACHU9J_001678"
     CDS             complement(20288..21610)
                     /locus_tag="ACHU9J_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905246.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="ACHU9J_001679"
     CDS             complement(22021..23034)
                     /gene="yhjD"
                     /locus_tag="ACHU9J_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1905247.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23083..23982)
                     /locus_tag="ACHU9J_001680"
     CDS             complement(23083..23982)
                     /locus_tag="ACHU9J_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1905248.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24502..>25103
                     /locus_tag="ACHU9J_001681"
     CDS             24502..>25103
                     /locus_tag="ACHU9J_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1905249.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5893 a         6366 c         6992 g         5852 t
ORIGIN      
        1 tagcttgtga atcaatttag aaggtcacaa gacgtgtgat tttagtcttt tcgtttttaa
       61 ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa ttcccgtctc gccgtaaaca
      121 ataaaccggc gaaatgctca cggttagaat tgtttcatga acggtacggt tatttcatag
      181 ggatcaagca aaatgaataa caatgaacca gatactctgc ctgatcccgc gataggctat
      241 atcttccaga atgatattgt ggcgttaaag caggcatttt cactgcctga tattgattat
      301 gccgatattt cccaacgcga acagttggcc gcggcattaa aacgctggcc gttgctggca
      361 gagtttgcgc aacaaaagta ggggattggt gaatggccgt actgggattg cagggggtgc
      421 ggggaggcgt ggggacaaca accatcaccg ccgcattagc ctggtcatta caaatgttgg
      481 gagaaaatgt cctggtggtc gatgcctgcc cggacaactt gttgcgcctg tcatttaatg
      541 ttgattttac ccaccgtcag ggctgggcca gagcgatgct ggatggccag gactggcgtg
      601 acgctgggtt gcgctacacc tcgcagctcg atttgctgcc ttttggtcag ttatccattg
      661 aagaacaaga aaatccacag cactggcaaa cccggctgag cgatatttgc tccggcttac
      721 agcaactaaa agccagcggg cgttaccagt ggattttaat cgacttaccg cgtgatgcct
      781 cgcagataac ccaccagctg ctgagtttgt gcgatcactc gctggcaatc gtcaatgtgg
      841 atgccaactg ccatatccga ctgcatcagc aagcgctgcc ggatggcgca catattttga
      901 ttaatgactt ccgtattggc agtcaggttc aggacgatat ttaccagctt tggttgcaaa
      961 gccagcgccg attactgccg atgctcattc atcgtgatga agcgatggct gaatgcctgg
     1021 cggctaagca accagtaggt gaatatcgca gtgatgcgct ggcggctgaa gagatactga
     1081 cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa aacgccagtc gggagtgcat
     1141 catgagtatc ctgacccggt ggttgcttat cccgccggtc aacgcgcggc ttatcgggcg
     1201 ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc gcgacgctcg gctgtttctg
     1261 gatgatcctg gcctggattt ttattccgct ggagcacccg cgctagcagc gtattcgcgc
     1321 agaacataaa aacctgtatc cgcatatcaa cgcctcgcgt ccgcgtccgc tggacccggt
     1381 ccgttatctc attcaaacat gctggttact gatcggtaca tcgcgcaaag aaacgccgaa
     1441 accgcgcagg cgggcatttt caggtctgca gaatattcgt ggacgttacc atcaatagat
     1501 gaacgagctg cctgagcgcg ttagccataa aacacagcat cttgatgaga aaaaagagct
     1561 cggtcatttg agtgccgggg cgcggcggtt gatcctcggt atcatcgtca ccttctcgct
     1621 gattctggcg ttaatctgcg ttactcaacc gtttaacccg ctggcgcagt ttatcttcct
     1681 gatgctgctg tggggtgtag cgctgatcgt acggcggatg ccggggcgct tctcggcgct
     1741 aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat atctggtggc gttacacctc
     1801 tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg cttattctgc tcttcgctga
     1861 aacgtacgcg tggattgtgc tggtgctcgg ctacttccag gtagtatggc cgctgaatcg
     1921 tcagccggtg ccattgccga aggatatgtc gctgtggccg tcagtggata tctttgtccc
     1981 gacttacaac gaaaatctca atgtggtgaa aaataccatt tacgcctcgc tgggtatcga
     2041 ctggccgaaa gacaaactga acatctggat cctcgatgac ggcggcaggg aagagtttcg
     2101 ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgc accactcatg aacatgcgaa
     2161 agcgggcaac atcaacaatg cgctgaaata tgccaaaggc gagttcgtgt cgattttcga
     2221 ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc atgggctggt tcctgaaaga
     2281 gaaacagctg gcgatgatgc agactccaca ccacttcttc tcgccggacc cgtttgaacg
     2341 caacctggga cgttttcgta aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca
     2401 ggatggcaac gatatgtggg acgccacttt cttctgcggt tcctatgcgg tgattcgtcg
     2461 taagccgctg gatgaaattg gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac
     2521 ttctctgcgt ttgcaccgtc gtggctatac ctccgcgtat atgcgtattc cgcaggcggc
     2581 ggggctggcg accgaaagtc tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg
     2641 gatggtacaa atcttccgtc tcgataaccc gctcaccggt aaaggactga agtttgctca
     2701 gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg ggcattccac ggctgatctt
     2761 cctgactgcg ccgctggcgt tcctgctgct tcatgcctac atcatctatg cgccagcgtt
     2821 gatgatcgcc ctattcgtgc tgccgcatat gatccatgcc agcctgacca actcgaagat
     2881 ccagggcaaa tatcgccact ctttttggag tgaaatctac gaaacggtgc tggcgtggta
     2941 tatcgcacca ccgacgctgg tggcgctgat taacccgcac aaaggcaaat ttaacgtcac
     3001 cgccaaaggt ggactggtgg aagaagagta cgtcgactgg gtgatctcgc ggccctacat
     3061 cttccttgtt ctactcaacc tggtgggcgt tgcggtaggc atctggcgct acttctatgg
     3121 cccgccaacc gagatgctca ccgtggtcgt cagtatggtg tgggtgttct acaacctgat
     3181 tgttcttggc ggcgcagttg cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg
     3241 cgtggagatg acgatgcccg cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac
     3301 cgttcaggat ttctccgacg gtggtttggg gatcaagatc aacggtcagg cgcagattct
     3361 ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag caggaatacg tcttcccgac
     3421 ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca
     3481 gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg gatacatggg cgctctggca
     3541 ggacagctac ccggaagata agccgctgga aagtctgctg gatattctga agctcggctt
     3601 ccgtggctac cgccatctgg cggagtttgc gccttcttcg gtgaagggca tattccgtgt
     3661 gctgacttct ctggtttcct gggttgtatc gtttattccg cgccacccgg agcggagcga
     3721 aacggcacaa ccatcggatc aggctttggc tcaacaatga tgataacgcg atgaaaagaa
     3781 aactattctg gatttgtgca gtggctatgg ggatgagtgc gttcccctct ttcatgacgc
     3841 aggcgacgcc agcaacgcaa ccactgatca atgctgagcc agctgtagcc gcccagacgg
     3901 aacaaaatcc gcaggtgggg caagtgatgc cgggcgtgca gggcgctgat gcgccagtcg
     3961 tggcgcagaa cggtccttcg cgtgatgtga agctgacctt tgcgcaaatt gcgccgccgc
     4021 cgggcagcat ggtgctacgt ggcattaacc cgaacggcag cattgagttt ggtatgcgca
     4081 gcgatgaagt ggtgacgaag gcgatgctca acctcgaata cacgccatcg ccatcgttac
     4141 tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga gctgatgggc gtgctgccag
     4201 tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat gcccattaac ccactgttta
     4261 ttaccgactt caaccgtgtg cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa
     4321 acccggccag caccacgctt tggctggatg ttgggcggag cagtggactg gatctgacct
     4381 atcagaccct gaatgtgaag agtgacctgt cacacttccc ggtgccattc tttgacccgc
     4441 gcgataaccg tactaacacc ttgccgatgg tctttgcggg tgcgccggat gttgggctgc
     4501 aacaagcatc tgccattgtc gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga
     4561 acttcccggt gctctataac caattgccgg atcgcaatgc cattgtcttt gcaaccaacg
     4621 acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa agccccggtg attgagatga
     4681 ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag
     4741 acctgttgca ggcagcgaaa ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg
     4801 tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg
     4861 tacgtaccga tcgtccggtc acatttggcg aactgaaaac ctatgaagaa cagttacaat
     4921 ccagcggtct tgagccagca gcgattaacg tttcgctaaa cctgccgccg gatctctacc
     4981 tgatgcgcag taccggcatt gatatggata ttaattaccg ctacaccatg ccgccggtga
     5041 aagacagttc gcggatggat atcagcctga ataaccagtt cctgcaatcc ttcaacctga
     5101 gcagtaaaca ggaggcgaac cgcctgctgc tgcggattcc ggtattacaa ggtttgctgg
     5161 atggcaaaac ggatgtctct attccggcgc tgaaactggg cgcgaccaac cagctgcgct
     5221 tcgactttga gtatatgaac ccgatgccgg gcggttcggt ggataactgt attaccttcc
     5281 agccggtgca gaatcatgtg gtgattggtg acgattccac catcgacttc tcgaagtatt
     5341 accacttcat cccgatgccg gatctacgcg cctttgctaa cgcgggcttc ccgttcagcc
     5401 ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa aacgcctaac gaagcacaga
     5461 tggaaacgtt gctgaatact gttggtttta tcggcgcaca gacgggcttc ccggcgatta
     5521 atctgacggt gaccgatgat ggcagcacca ttcagggcaa agatgccgac atcatgatca
     5581 tcggtggtat cccggacaaa ttaaaagacg ataagcagat cgacctgttg gtgcaggcga
     5641 ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg
     5701 agagcgatcg cgcggcagaa acccagtcaa cgctgacctc ttccggtgcg atggcggcgg
     5761 tgattggctt ccagtcgccg tataacgacc agcgcagcgt gattgcgctg ctggcagata
     5821 gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga tagcggcaaa cgcgccacca
     5881 tgttcggttc ggtcgcggtg ctccgcgagt ccggtatcaa cagcctacgt gttggcgacg
     5941 tttattacgt aggtcatctg ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc
     6001 cgattctgct ggcggtgctg gcggcaatca gtgtgatatt gctggcatgg gtactgtggc
     6061 gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga
     6121 atgtgttgcg tagtggaatc gtgacgatgc tgctgttggc tgcctttagt gttcaggcag
     6181 cctgtacctg gcctgcctgg gagcagttta aaaaggatta catcagtcag gaagggcgcg
     6241 tcatcgcccc cagcgacgcg cgcaaaatca ccacctccga agggcaaagt tacggtatgt
     6301 tctttgccct ggcggctaac gaccgtgtag ctttcgataa tattctcgac tggacgcaga
     6361 acaatctcgc tcagggctct ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag
     6421 agaacagtaa gtgggaagtg ctggacagca attcggcctc cgatggtgat gtctggatgg
     6481 cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca gcgttatacc gacatcggca
     6541 gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca
     6601 tgttgttacc gggcaaagtg ggttttgctg aggataacag ctggcgtttt aaccccagct
     6661 acctgccgcc gacgctggcg cagtatttca cccgctttgg cgcgccgtgg accacgctgc
     6721 gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa aggtttttcg ccagactggg
     6781 tgcgctatga gaaagacaaa ggctggcagc taaaagccga aaaaacattg atcagcagct
     6841 acgacgctat ccgcgtttac atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag
     6901 cgcggatgct caaccggttt aaaccgatgg cgacattcac tgagaaaaac ggttatccgc
     6961 cggaaaaagt ggatgtggct acggggaaag cgcagggtaa aggaccgatc ggtttttctg
     7021 ccgccatgct gcccttttta caaaaccgcg atgcgcaggc cgttcagcgc cagcgcgtgg
     7081 ccgataactt tcccggcagc gatgcctatt acaactatgt gctgaccctg tttggacaag
     7141 gctgggatca acaccgtttc cgcttctcga caaaaggtga gttattacct gactggggcc
     7201 aggaatgcgc aaattcacac taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc
     7261 gatggtcgag gcagcaccaa ccgctcagca acagttgctg gagcaagttc ggttaggcga
     7321 agcgacccat cgtgaagatc tggtgcaaca gtcgttatat cggctggaac ttattgatcc
     7381 gaataacccg gacgtcgttg ccgcccgttt ccgttctttg ttacgtcagg gcgatattga
     7441 tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg ccgagttcaa atgcgtataa
     7501 atcgtcgcgg actacgatgc tactttccac gccggatggt cgtcaggcac tgcaacaggc
     7561 acgattgcag gcgacgaccg gtcatgcaga agaagctgtg gcgagttaca acaaactatt
     7621 caacggtgcg ccgccggaag gtgacattgc tgtcgagtac tggagtacgg tggcgaaaat
     7681 tccggctcgc cgtggcgaag cgattaatca gttaaaacgc atcaatgcgg atgcaccggg
     7741 caatacgggc ctgcaaaaca atctggcgct attgctgttt agtagcgatc gccgtgacga
     7801 aggttttgcc gtcctggaac agatggcaaa atcgaacgcc gggcgcgaag gggcctctaa
     7861 aatctggtac gggcagatta aagacatgcc cgtcagtgat gccagtgtgt cggcgctgaa
     7921 aaaatatctc tcgatcttta gtgatggcga tagcgtggcg gctgcgcaat cgcaactggc
     7981 agaacagcaa aaacagctgg ccgatcctgc tttccgcgct cgtgcgcaag gtttagcggc
     8041 ggtggactct ggtatggcgg gtaaagccat tcccgaacta caacaggcgg tgcgggcgaa
     8101 cccgaaagac agtgaagctc tgggggcgct gggccaggcg tattctcaga aaggcgatcg
     8161 cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg gacccgcaca gcagcaacaa
     8221 cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg ctggcgatcc agcagggcga
     8281 tgctgcgctg aaagccaata atcctgaccg ggcagaacgc ctgttccagc aggcgcgtaa
     8341 tgtcgataac accgacagtt atgcagtgct ggggctgggc gatgtggcga tggcgcgaaa
     8401 agattatccc gccgccgaac gttattatca gcagaccttg cgtatggaca gcggcaacac
     8461 taacgccgtg cgcgggctgg caaatattta ccgccagcaa tcgccagaaa aagctgaagc
     8521 gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt gatgatatcg aacgcagcct
     8581 gcaaaacgac cgtctggcac agcaggcaga ggcactggaa aaccagggca aatgggcgca
     8641 ggcggcagca cttcagcggc aacgactggc gctggacccc ggcagcgtat ggattactta
     8701 ccgactttcg caggatctct ggcaggccgg acaacgcagc caggccgata cgttaatgcg
     8761 caatctggcg cagcagaagt cgaacgaccc agagcaggtt tacgcttacg ggctgtacct
     8821 acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg gcgcatatca
     8881 acagcctgcc gcgtgcgcag tggaacagca atattcagga gctggttaat cgactgcaaa
     8941 gcgatcaggt gctggaaacc gctaaccgcc tgcgagaaag cggcaaagag gcagaagcgg
     9001 aagcgatgct gcgccagcaa ccaccttcca cgcgtattga cctcacgctg gctgactggg
     9061 cgcaacaacg acgtgattac accgccgccc gcgctgcata tcagaatgtc ctgacgcggg
     9121 agccagctaa cgccgacgcc attcttggtc tgacggaagt ggatattgct gccggtgaca
     9181 aagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc tcgctgaaca
     9241 cacagcggcg cgtggcgctg gcacaggcgc agcttggcga taccgcagca gcgcagcgga
     9301 cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa agcgcgatgg
     9361 tgctgcgtga tggtgcgaag tttgaagcgc aggcgggcga tccaacgcag gcgctggaaa
     9421 cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag gataacgaca
     9481 cctttacccg actgacccgt aacgacgaga aagatgactg gctgaaacgt ggcgtgcgca
     9541 gcgatgcggc ggacctctat cgccagcagg atcttaacgt cacccttgag cacgattact
     9601 ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc atgttgcagg
     9661 tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc aatatgaacg
     9721 tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc acctgtacat
     9781 tacaggactg tagcggcaac cgcagccagt cggattccgg tgccagcgtg gcggtcggct
     9841 ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc aacgtggtgg
     9901 atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac accgttaacg
     9961 cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa gactccccga
    10021 gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta agtctgagct
    10081 acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag ttaaccggta
    10141 aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat aaggtcatta
    10201 accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat tacgacaaag
    10261 atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa tacctgtcgt
    10321 ttgccatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag ctgggggcgt
    10381 ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg atgaatctga
    10441 tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc agcagtcagg
    10501 gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac tggtttgttg
    10561 gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc ctgctctacg
    10621 tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag ccgctgatac
    10681 cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc gatcgggtat
    10741 actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt cgctcgttaa
    10801 caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt attttttgca
    10861 ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg caactggaaa
    10921 gcattgctcg ctctgtccgc gaacccttat cttcagctat tttgaaaggc gatattcccg
    10981 aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt gccgatgtag
    11041 tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc ccggtgccga
    11101 taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt tactcgctcg
    11161 aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat tccttccgta
    11221 tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta cttttgtcgc
    11281 ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat ccgttacgca
    11341 atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac caactggcat
    11401 taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac aacctcaacc
    11461 agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc ttcccggtgt
    11521 cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc gcgcgtaaac
    11581 aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg ggcgtgctga
    11641 aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa tcggtactgt
    11701 cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt gccaacggtg
    11761 tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcactatc atgagcgagc
    11821 gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg gcgatgttct
    11881 acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca tttaccgctc
    11941 gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa gccgcccaga
    12001 agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag tttgctattt
    12061 ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa gtgttactgc
    12121 gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat cgcattgagt
    12181 gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt cgattgcttg
    12241 cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct gcgctgcaac
    12301 tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat cgcattcagc
    12361 cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct catgctgcgg
    12421 tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat gatttcggca
    12481 tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc gacgtactga
    12541 aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt gctgcaatta
    12601 tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag actgaagcac
    12661 aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt tttgctcacc
    12721 cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta ccccaaactg
    12781 attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt taaccttttt
    12841 gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt ttaaggccgc
    12901 aggtacccca taaccttaca agacctgtgg ttttactaaa ggacacccta tgaaaacctc
    12961 tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta ttctccttgg
    13021 ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct
    13081 cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat
    13141 ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt
    13201 cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg
    13261 aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc
    13321 gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggcgagcg tcattggcgc
    13381 atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct
    13441 ccaccgtctg ggcagcaaag gccaactgat ttttaacgtc atcgaaagtt tctcgcaggt
    13501 catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt tcggggcaat
    13561 ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc agctgattat
    13621 ctgtttctat attacctgta tcctgtttgt ggtgctagta ttgggttcaa tcgctaaagc
    13681 gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc tgattgtact
    13741 ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg
    13801 ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta accttgatgg
    13861 cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta acagtcagat
    13921 ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta aaggggcggc
    13981 aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt
    14041 gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc
    14101 gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt gggtgaaaga
    14161 actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg gcaaaacgca
    14221 cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag tgcgggtgtt
    14281 tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg tttatttcac
    14341 ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta acgtcattta
    14401 gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg ttcacatgca
    14461 gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg ctggctatgt
    14521 gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt ccaacggttt
    14581 acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct
    14641 ggttaatatc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc acgccatccc
    14701 tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt cattgtggca
    14761 gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg acaccacgct
    14821 gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc tctcttatct
    14881 ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg cgctgcaaag
    14941 tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc gctatcgtct
    15001 gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc
    15061 ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga cgctactggt
    15121 ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact
    15181 gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc gtgcggaagc
    15241 ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt gggatacgcc
    15301 gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg acctggcccg
    15361 cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca aagacatcgg
    15421 tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc
    15481 accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg
    15541 cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga aactggagct
    15601 gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg gtcagcggat
    15661 gcgttcgctg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga aactgcggca
    15721 ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc agcagttgtc
    15781 gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta acatgaaagc
    15841 gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg ccacctctgt
    15901 cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt aatgcctgat
    15961 ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag gggtaacact
    16021 ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg ggataatcgc
    16081 gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg cgttttccgc
    16141 actgccgccc gtcaaacata ccgccagata accggcgctg aaagagtcgc cagctgcggt
    16201 ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta acccttcgcc
    16261 agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt tcacgcccgc
    16321 gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg cgtcttcatc
    16381 gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca cctgctgtgt
    16441 ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca cttttccgcc
    16501 gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg ggcttaagat
    16561 cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt cgcaaatcgc
    16621 cgcagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc agtagtagaa
    16681 cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac ggttttccat
    16741 ccgttgggtc agggaagtat caacgttctc gccgtgccag gcgtccagca tttgctggct
    16801 aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag gatcgacctg
    16861 acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct taacgtccgc
    16921 gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt tggacatagt
    16981 cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt gctggtcaac
    17041 catattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga aagattccgt
    17101 tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttgc gcgccgataa
    17161 tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat gataaggcag
    17221 gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga acggcgtttt
    17281 tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg cggacggtta
    17341 atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca acgcctgccg
    17401 ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt gaaagagcag
    17461 attgatttgc tggcgcaaaa agccgacttc tttgtagagc acgacctgct ggcatcggtc
    17521 aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct gcgccaaatt
    17581 gagcgtcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc gaaagattca
    17641 acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg caccgggatg
    17701 gcaaatttct ctgcgctaag tgaagtgcgt tatgactaca tcaaaatcgc gcgagagctg
    17761 ttcgtgatgc tgcgtcagtc gccggaaggc cgcacactct tttctcagct tttacatcta
    17821 atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga agagtggcgt
    17881 gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg cccggcaccg
    17941 atggaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc cgctgggctg
    18001 gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag caaggcaggc
    18061 aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc gatcattttg
    18121 attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt ctctgcggag
    18181 ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaaacaa
    18241 gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga catcattctt
    18301 ggtaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt agaggcaacg
    18361 ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa gctcgaaaag
    18421 cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt taatcttgcc
    18481 aacgatgaca acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat
    18541 attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc ggatctggaa
    18601 atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg atcgaaaggt
    18661 aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca gggacgttat
    18721 aacggtgaac cgctcacggg tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa
    18781 gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt tgcttttgat
    18841 ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa attttctggc
    18901 gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac cccgccgttt
    18961 gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt ctttgattat
    19021 cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt ctacaccacc
    19081 ggaaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac
    19141 ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa acggtctgaa
    19201 cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc
    19261 gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg gcgcattgag
    19321 catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa aaatgctgac
    19381 ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc gaatattcat
    19441 ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg tcgactgaaa
    19501 ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga aatgaacggt
    19561 gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa cagtaacggc
    19621 aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga gattgttggg
    19681 ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac
    19741 tgcgcggcgg cgaatctgaa tattgccaac ggcgtggcgc gcccgcagat ttttgctttc
    19801 gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc agaacagctg
    19861 gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg ttcgccgctg
    19921 tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc gctgattgcc
    19981 cgtggtgctg ttgctgcggc actggcaacg ctggtaacac cgacggcggc gttactggca
    20041 ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc gcagatgaag
    20101 aagtgatgat aattgccgga tgtctgacat ccggcaatta aacagattta gatttttggc
    20161 aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaatcctt
    20221 gtgaattcaa tatattgtat ggaaattcag tcctgataag cgtagcgcat caggcttttc
    20281 acacttatta caacgactga tgtcgcgtct catgggtcag cagcagggcg attaacgtca
    20341 agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta gtctgcaacc
    20401 aggctgcgat atacggtgca acggaagccc cgagaatcga cgctacgttg taagagaatg
    20461 atgctccggt gtaacgcact tctgttggga acagctctgg taacagcgcg cccatcggcc
    20521 cgaaggtcag ccccatcaga cttaacccca gcagcaggaa ggcaaaaacc agaatcgggt
    20581 tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc agcgtggtga
    20641 taattaccat gcttttacgg cgaccaaagg catcagccag taatccggcg actggcacca
    20701 tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta cgcggcaggc
    20761 caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc atgatgtaaa
    20821 acagcgtata ggttgccagc atgatgaacg tacccagtac ggttacgcga acatgtttgg
    20881 tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact ttctcaaaca
    20941 ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagt accgccgaga
    21001 agataaatgg cacacgccag ccccagctca taaactgctc gtcggtcagt agccaggaaa
    21061 gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc tgtgggaagg
    21121 agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc gccgcgccgc
    21181 cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc agcagcgggg
    21241 cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtt gaaatcccca
    21301 tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca aaatgaccaa
    21361 aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg agcgactgta
    21421 gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca ataacggccg
    21481 cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga gaggcgacaa
    21541 ggactttatt acgcgagttg atcggcgtgt attcttgctc gtggtcgagt gttgtggctg
    21601 ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac gcacagcaaa
    21661 agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca aaaaggggat
    21721 aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta taaatagtgg
    21781 ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct ggggttatga
    21841 gttggaatga aattgtttgc tcccaaatag atggctgaag aggctctggg gaagagtttc
    21901 aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc cggcctacgg
    21961 ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat ccggcctatt
    22021 ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg tggcaatcca
    22081 cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca gccccagcac
    22141 ggaaccaaat gctgcgccag agggggattt catcagcgat ggcagggtgt aggtcatcac
    22201 gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca gggctttttt
    22261 gcgcggacgg tggcgcggta accgccagaa gatccagaag aaaagcagat agttggcgaa
    22321 gatggaaatc gccaggccaa tcaaccgcca cgtcggtttc agccactcaa tgctgttcag
    22381 gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga tcgaaagcgt
    22441 cacaatcagc gcaatcaaca aaccaatcag cgaaataaaa tcacgtagat attttaccca
    22501 gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg cgcgaatcgc
    22561 ttcacgcagg ttacccatcc agttgatgcc ggaataaagc gccacagcca ggccgacaag
    22621 ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg tggcggctag
    22681 cgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca acatcggatg
    22741 ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga tcatcgacaa
    22801 aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt taaagcgttc
    22861 tgtcgcgcga atcaggtgcg caataaccgg ttggcgttgg actttttcgg cagtggtggt
    22921 gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca actgcttaat
    22981 cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg tcatccgctc
    23041 ttttgtcctt ctgttgtggt cattcttaaa aagtatagtc ggtcagtcca ggtactcttt
    23101 cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct cccgacgctg
    23161 cggataaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca cttcaataag
    23221 ctgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa tccccagacc
    23281 ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc cgccagactt
    23341 aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta cacccaggtg
    23401 cggtgtataa cgcaccattg cgtgtgaagt cagatcatcg gggctttgcg gataaccaaa
    23461 gcgcgtcaga tagtgcggac tggcacagtt gaccatggtc agtttgccga ggggacgggc
    23521 gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac catcatgaag
    23581 aatatctacc ggacggttat ggctactcag ttccagctca attcccggat gctgatagag
    23641 aaattccgac agacgcggca gtaacaggtt tttcgcgatt cctgacggga tgtcgatgcg
    23701 taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt cgctgagatt
    23761 actcaacaca tcttttgccc gttgataata ggtcatgcct tctggcgtga gtttgacccg
    23821 tcgcgtagtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct gtatctggcg
    23881 cgaaacactt ccctttggca aagcaaagaa atccgccgcg cgggaaaaac tttccagctc
    23941 cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga ccgattgttg
    24001 ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta acatctaatg
    24061 ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa aagggcggaa
    24121 tagctttatg agaaaggtga atccccttaa acagagcgta tttttcagtg attcccttta
    24181 aagtgaatag aggacgtatt ggctatttgt gcttattttt gtctcattgc catttgttat
    24241 tttggcttgt agtcgcggaa tttcattttt aattgcatgc gtactaacca ccaatataag
    24301 gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc gtttagctgt
    24361 aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc caccgccaaa
    24421 tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt ctttttaacg
    24481 tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc aatatttatt
    24541 catggaatga aaatcagttt acagcagcgt attccaggag tgagtattca gggggccagt
    24601 caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt tatgctcgat
    24661 ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt gcagtttcct
    24721 gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca ggaagttatt
    24781 cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt tgcgttggcg
    24841 gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac gacgccggaa
    24901 aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt aaccatgtta
    24961 gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac cggaacggta
    25021 aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac ccaggcggca
    25081 atgatgttaa atatctcctc ctg
//
LOCUS       DAZPJP010000045        24462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPJP010000045 DAZPJP010000000
VERSION     DAZPJP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.045"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(166..576)
                     /locus_tag="ACHU9J_001682"
     CDS             complement(166..576)
                     /locus_tag="ACHU9J_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1905250.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="ACHU9J_001683"
     CDS             complement(634..867)
                     /locus_tag="ACHU9J_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1905251.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="ACHU9J_001684"
     CDS             1256..1801
                     /locus_tag="ACHU9J_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1905252.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="ACHU9J_001685"
     CDS             1776..3701
                     /locus_tag="ACHU9J_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1905253.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="ACHU9J_001686"
     CDS             3698..3904
                     /locus_tag="ACHU9J_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1905254.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="ACHU9J_001687"
     CDS             3901..5502
                     /locus_tag="ACHU9J_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1905255.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="ACHU9J_001688"
     CDS             5483..6802
                     /locus_tag="ACHU9J_001688"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1905256.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="ACHU9J_001689"
     CDS             6812..7144
                     /locus_tag="ACHU9J_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1905257.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="ACHU9J_001690"
     CDS             7200..8225
                     /locus_tag="ACHU9J_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1905258.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="ACHU9J_001691"
     CDS             8267..8665
                     /locus_tag="ACHU9J_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1905259.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="ACHU9J_001692"
     CDS             8677..9030
                     /locus_tag="ACHU9J_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1905260.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="ACHU9J_001693"
     CDS             9042..9620
                     /locus_tag="ACHU9J_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905261.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="ACHU9J_001694"
     CDS             9617..10012
                     /locus_tag="ACHU9J_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1905262.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="ACHU9J_001695"
     CDS             10020..10760
                     /locus_tag="ACHU9J_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905263.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="ACHU9J_001696"
     CDS             10776..11198
                     /locus_tag="ACHU9J_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1905264.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="ACHU9J_001697"
     CDS             11180..11614
                     /locus_tag="ACHU9J_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1905265.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="ACHU9J_001698"
     CDS             11607..14168
                     /locus_tag="ACHU9J_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1905266.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="ACHU9J_001699"
     CDS             14165..14494
                     /locus_tag="ACHU9J_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905267.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="ACHU9J_001700"
     CDS             14494..15192
                     /locus_tag="ACHU9J_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1905268.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="ACHU9J_001701"
     CDS             15198..15941
                     /locus_tag="ACHU9J_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1905269.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15938..16510
                     /locus_tag="ACHU9J_001702"
     CDS             15938..16510
                     /locus_tag="ACHU9J_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1905270.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="ACHU9J_001703"
     CDS             16571..20068
                     /locus_tag="ACHU9J_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1905271.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="ACHU9J_001704"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="ACHU9J_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..23803
                     /locus_tag="ACHU9J_001705"
     CDS             20720..23803
                     /locus_tag="ACHU9J_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1905272.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23803..24319
                     /locus_tag="ACHU9J_001706"
                     /pseudo
     CDS             23803..24319
                     /locus_tag="ACHU9J_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         5801 a         6104 c         7432 g         5125 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgcct
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gcgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcgcagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacatc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacatcgt ggcatctcgt
    23941 tgaagatcat cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt tatttatttc
    24001 tgaactcggc tcattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa
    24061 gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc ggatccggga
    24121 ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag cgccgcttca
    24181 ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg aagcctggaa
    24241 aaagtatcgg gtgttgctga accgtgttga tacatcaact gcaccggata ttgaatggcc
    24301 agtagcacct atagggtaaa ttcgtaatga ttacctaaat acgttattct tttgttaaaa
    24361 aagtgatttt gctataggta gggagtatgt tcttggatta agaataaaaa atcgccatga
    24421 aagagcttca tggcgagtac ataaacgtac aaaatgtgat ga
//
LOCUS       DAZPJP010000046        24395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPJP010000046 DAZPJP010000000
VERSION     DAZPJP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.046"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            13..729
                     /locus_tag="ACHU9J_001707"
     CDS             13..729
                     /locus_tag="ACHU9J_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1905273.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            759..1259
                     /locus_tag="ACHU9J_001708"
     CDS             759..1259
                     /locus_tag="ACHU9J_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1905274.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1351..4035
                     /locus_tag="ACHU9J_001709"
     CDS             1351..4035
                     /locus_tag="ACHU9J_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1905275.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4032..5120
                     /locus_tag="ACHU9J_001710"
     CDS             4032..5120
                     /locus_tag="ACHU9J_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1905276.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5203..6500)
                     /gene="exuT"
                     /locus_tag="ACHU9J_001711"
                     /pseudo
     CDS             complement(5203..6500)
                     /gene="exuT"
                     /locus_tag="ACHU9J_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            6983..8395
                     /gene="uxaC"
                     /locus_tag="ACHU9J_001712"
     CDS             6983..8395
                     /gene="uxaC"
                     /locus_tag="ACHU9J_001712"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1905277.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8410..9897
                     /gene="uxaA"
                     /locus_tag="ACHU9J_001713"
     CDS             8410..9897
                     /gene="uxaA"
                     /locus_tag="ACHU9J_001713"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1905278.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9980..10531
                     /locus_tag="ACHU9J_001714"
     CDS             9980..10531
                     /locus_tag="ACHU9J_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1905279.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10536..11780)
                     /gene="sstT"
                     /locus_tag="ACHU9J_001715"
     CDS             complement(10536..11780)
                     /gene="sstT"
                     /locus_tag="ACHU9J_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1905280.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12396..13361)
                     /gene="alx"
                     /locus_tag="ACHU9J_001716"
     CDS             complement(12396..13361)
                     /gene="alx"
                     /locus_tag="ACHU9J_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1905281.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13644..14630)
                     /locus_tag="ACHU9J_001717"
     CDS             complement(13644..14630)
                     /locus_tag="ACHU9J_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1905282.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14709..15392)
                     /locus_tag="ACHU9J_001718"
     CDS             complement(14709..15392)
                     /locus_tag="ACHU9J_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1905283.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15469..15972)
                     /locus_tag="ACHU9J_001719"
     CDS             complement(15469..15972)
                     /locus_tag="ACHU9J_001719"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1905284.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16057..17193
                     /gene="rlmG"
                     /locus_tag="ACHU9J_001720"
     CDS             16057..17193
                     /gene="rlmG"
                     /locus_tag="ACHU9J_001720"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1905285.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17478..17792
                     /gene="higB"
                     /locus_tag="ACHU9J_001721"
     CDS             17478..17792
                     /gene="higB"
                     /locus_tag="ACHU9J_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1905286.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17789..18205
                     /gene="higA"
                     /locus_tag="ACHU9J_001722"
     CDS             17789..18205
                     /gene="higA"
                     /locus_tag="ACHU9J_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1905287.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18250..20268)
                     /gene="fadH"
                     /locus_tag="ACHU9J_001723"
     CDS             complement(18250..20268)
                     /gene="fadH"
                     /locus_tag="ACHU9J_001723"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1905288.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20494..22845)
                     /gene="ygjK"
                     /locus_tag="ACHU9J_001724"
     CDS             complement(20494..22845)
                     /gene="ygjK"
                     /locus_tag="ACHU9J_001724"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1905289.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNDNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22862..23939)
                     /gene="ygjJ"
                     /locus_tag="ACHU9J_001725"
                     /pseudo
     CDS             complement(22862..23939)
                     /gene="ygjJ"
                     /locus_tag="ACHU9J_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24073..>24395)
                     /locus_tag="ACHU9J_001726"
     CDS             complement(24073..>24395)
                     /locus_tag="ACHU9J_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905290.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6160 a         6195 c         6253 g         5787 t
ORIGIN      
        1 gatcaaattt atatggtggc gaaaattaat ctcttatcac ggcttacgcc gcttttattc
       61 gtgttcgctc cttttttggc acagtcaaat atgactgttt atcctatggc tgtttccatt
      121 aatagtcagg gtgaaggcaa cgttcgcgta atatctaaaa gtaatgaagt tcagtacatc
      181 aaggcgacag tattccgtat cgataatcca tcgacgcctc aggaaaatga agttgagatt
      241 aaatccggtg atgcaaatca tctggttgtt atgccaccta aattcgcttt accagccggt
      301 agtagcaaaa ctgtgcgctt tgttgcaatg gaaccagagc aaaaagaaaa aaattatcgc
      361 gttaaatttg aagcggttcc cagtattgat gacgttgcca cagataaaaa agatctctct
      421 atgcagttaa cagttaactt aatttggggg attgttgtta gtgttccacc tcagcaacct
      481 attgctaagt tagaagtaaa tgctgcccaa aaattagtta atgcagggaa tcaacgctta
      541 aagattttaa cgatcgctta ttgtaaaaat aatagcaaag aaaattgtaa gatacagacc
      601 gtaaataaaa acatcttcct tggtcaggaa agaaatcttg aaagcatatc tggctacgac
      661 aaaatcgttg tcaaatataa taactggatc accaaagata atggcgagtt tgaactggcg
      721 gtccattaat tatcattgta ataaacgaag gaatagtaat gaaaaaggta tttgcaaaat
      781 ctcttctggt cgcagcgatg ttttctgttg caggctccgc attggcagta caaaaggata
      841 ttactgtaac ggccaacgtt gatgccgctc tggatatgac acagaccgat aacaccgcgt
      901 tgccgaaagc agtagaaatg caatatctgc cggggcaggg tctgcaatct taccagttga
      961 tgaccaaaat ctggtctaac gacgttacca aagatgtaaa aatgcagtta gtttccccgg
     1021 cgcaactggt tcaaagcctg gatgctagca agattgttcc gctgacagta acctggggcg
     1081 gtgaagaaat taaagctgat gcggcaacca cttttaccgc aaccaaaatc tttgcttccg
     1141 atgcgttaac taacggttct ctggctaaaa acctgatgtt tgctcaaacc accaaaggtg
     1201 ttctggagac aggcatctac cgtggtgtag taagcattta tctgtctcag gatatctaat
     1261 ccagataaca gaattaacac gtaacaaagg gaggggtagc cctcccttat tttaactgtt
     1321 ttagtatttg ttatggattt caggattgcc atggataaaa aattactggc tcttttgatc
     1381 ctggcgagtc tcagcccggc agaggcgaca ttaaccgaaa ttcccgcagg gtttgaggtt
     1441 attgctcagg gacagcagga gtatatcgag gtttattttt cagggaaaag tctcggtaaa
     1501 tattatgcaa tggttaatct tgataccgta acatttcttg atccagcaag tctatataac
     1561 aagctggaac tggatgtaga cgatcagaaa atcgcgcata tagtgaaaga aaaattatcg
     1621 cagccgctag ctcgccacgg tgaattggct tgcggttatg tacgtactga ctcagggtgt
     1681 ggttttctga ataccgatac gctggaaata atctataatg atgaagaaag ttcggcaacg
     1741 ttgtttatta atccgcaatg gaattcagct ttcgatgcga agtcattata tttaaatcca
     1801 gacaaaaata ctgttaatgc ttttatacat cagcaagaca tcaatgttct ggcacaggat
     1861 gattaccaat cgttgtctat tcagggaaac ggtgcgctgg gaataacaga aaatagttat
     1921 attggtgcac actggaattt caacggttat gatgcagatg atgtcagtga cagtaatgct
     1981 gacgtcagcg atctctatta tcgttatgat tttttacgtc gttattatgt gcaggcgggt
     2041 cgcatggaca accgcacact atttaatgca caaggcggga actttacctt taactttctg
     2101 ccactcggtg caattgatgg gatgcgtatc gggtcgacac tgagctattt aaaccaggcg
     2161 caaagccagc aaggaacccc agtaatggtt ctgctttcgc gcaattctcg tgttgacgct
     2221 tatcgtaatg agcaactttt gggatcgttt tatctcaata gtggttcgca atttattgat
     2281 accagttcct ttccgccagg cagctacagc gtggcattaa aagtctacga aaataaccaa
     2341 ctcacccgca ccgagcttgt gccgtttacc aaaaccggcg gtctgactga cggaaatgcg
     2401 caatggttct tacaggcagg taaaactaca tcacaggttt ctgatgatga aagttcagct
     2461 tatcagctag gagtacgcct gccattacat ccgcaatatg agctctacgc agggctggcg
     2521 aatgccgatg atgtgagtgc tttcgagtta ggtaataact ggacggcaga tttaggcggg
     2581 gtggggaatc ttgcaatcag cgccagcgtg ttccgtaacg atgacggcgg caaaggtgat
     2641 atgcaacagg ccaactggag taatccggga tggccgacgt tgggctttta tcggaccaac
     2701 tctgacggcg atgcttgtac aaccgacagc agagagagct ataacgcctt aagctgttat
     2761 gaaagtattt ccgcgacggt ttcacagaat tttgtcggct ggaatatgat gctgggttat
     2821 acccgcacac aaaataacac tgatgatagt ttgcgttggg ataaacagca gagctttgaa
     2881 aataactatc ttcgccagac aacagcgcaa agtatctctg aaactgtaca acttagcgct
     2941 tcccgcgctt ttgtgatgcg tgactggatt ttgagtacgt cagtcggtgt tttccatcgt
     3001 aatgacaacg gtggcgataa cgacgacaac ggcttgtact tatcgttttc gttatctgac
     3061 acgccaacga tggatagcaa taacaacagc cattcaacaa atgtgtctac ggattatcgt
     3121 tatagcgaac aggatggcga tcaaacgtca tggcagttat cgcatacctt ttataacgat
     3181 tcattcagcc ataaagaact tggcgtaacc gtcggaggcc tgaacaccga taccataaac
     3241 agcgcggtta acgggcgttg ggatgggcaa tacggaaatg tctacgctac cgtatctgat
     3301 agttatgacc gtaagaatca tgatcatctt tcggccttta cgggcactta tagctctacg
     3361 ctggctgtca gttgctatgg cgttaatttg ggagccagtg gtacagacga tttgctgggt
     3421 gcggtactgg tggatgtgaa aggcttctct gaacaggatg aagagagtca ggatctgcaa
     3481 ctcgaagcgc gggtggcagg cagccgaact ttgcagcttg gtcaaagtga cagtgtgttg
     3541 ttcccttatc ctggatttca gtctggtttt gttgaggtta acgacagtag ccagggcaat
     3601 cagcagggga caacaaacat cattaacggt gcagggaatc gtgaattaat gttgttgcct
     3661 ggcaagctgc gctatcgcga agtgtctgcc agctttaact acaactatat cggtcgttta
     3721 ttactgccgg cagcggtgaa aaaattcccg attgtggggc tgaacagcgc catgttactg
     3781 gtagctgaag atggaggatt tacacttgaa attaacggca gcgaaaaaga gctctatctg
     3841 ctttccgggc agcaattcct taagtgtcca ctgagtgttg taaagaaacg cgccagcatt
     3901 cgttacagcg gagatgttac ttgtagtgtg gtgacttatt cacaattacc ggagtcgatt
     3961 caggttcagg cacagttgaa acagcctaaa ttacgtggaa acgttcagac ggcgcaaagg
     4021 gaggttgcac catgagaaat cgattgattg cggcgatatt gggcttgttt ggcacgctca
     4081 ttggcgttca ggcagctcct gacgtgacca gtgaaattac gtatgatttg gcatctggta
     4141 gagcggatta ttacttctgg aaggatgaag cgtcggcagg aaataatgga tatatgtggt
     4201 atgaatgttc gtatcctgac ctccaacaaa cctgtacagc taatggaaat atatcgacag
     4261 tacaaatcta tttaactgaa caacgcagtg ggatgcgttg gccggtaaaa ctcaaaggat
     4321 ttaaaacagc cattgtaagt agcgatgaag cgccgccagg atgcaagggg ggcaaagggc
     4381 ttcagacgaa tcttaaggat tctaatagat cttcatgtac agaagatggt caacattatt
     4441 atatatacga tacaaagttt cttacgctct accttgagca gacagagatg aagaatttgc
     4501 cgattggtgg cgtctggaag gggaaagtta aattacattc gaacagcccg gcccaggact
     4561 atttcgcaaa tattaccctg aatacgctcg accccaacca tattgacgtg ttcttcccgg
     4621 agttcgccca cgccacgcca agggtgcagt tagacttgca tccaacagga agcgttaacg
     4681 gcagcaacta cgcgcaagat ctgaccatgt tggacatgtg cctgtacgat ggttttaacg
     4741 gtaatgccat cagttatgaa atcatgctca aagatgaagg gcgaccagct gcagggcgca
     4801 gagacggtga cttctctatc tatcgtcaag gagggaccac caccgacgag ggagaacgca
     4861 ttgattaccg ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat gtgcgcaata
     4921 atgaaaatat ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg gtactgcccg
     4981 gtattcgcta tgccgtgatg tgtgtgccga cacccttgac tctggcagtc gataaattca
     5041 gcgtgatgga taaacaggcc gggtactaca tgggcaaatt gtcagtaatc tttacgcctt
     5101 ccttgccaac catcaattga ccccgcagac cagaaaaaac taaacaacga agccgcctga
     5161 taaggcggct tcgtcattac aacttacgtt aaacgttaaa cgttaatgtt gcggtgcggg
     5221 atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaata cggtccagat
     5281 aaccagcgca cccagcaggt caaatactgc cagaactgcg aacagcgggc tgaagccgat
     5341 ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat
     5401 cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag aagagagcgt
     5461 aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc
     5521 gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca cagcacccag
     5581 cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg
     5641 cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa
     5701 catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc
     5761 gttaaaagta ccccaggccg gttctgccag gaaacgcggc agtgcgatac cccagaactg
     5821 acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc
     5881 ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg
     5941 atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat
     6001 aaatgccatc tgccagctgt gcatcacgat tgccatacca ccagcggcgg cgcaatcatc
     6061 gcaccaatcg aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg
     6121 aaccattcgg agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc
     6181 gcaccacgag caacagccag gccaccccag ctacctgcca gcgcggttac accacagaac
     6241 acggcccaca gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca
     6301 taacctgcta ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa
     6361 tactgttggg tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg
     6421 ttacgcgtca ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt
     6481 aaccctttaa ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg
     6541 ccatcatccc gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaccgg
     6601 cacgccccga acgttgccat gaaagtttcg tgctaattcg gcttccgtac cggttaaccc
     6661 gattcatcta acaaatgaat gaatcggcaa tttgcattcc gtcgacttat gatttgcgac
     6721 ggcagaaaga taacttgtca tacaacttta aaaggtgaga gccatcacaa atgtgcgaat
     6781 atttgtaggg acattacctg acgacagcga ggccagtact ggcgcggcct gcagcgagat
     6841 ttatcacttt gagagtaatt tttttaacta cgtttattga tctaactcac gaaaatatct
     6901 tcggactctg gaaattggtg tgataacttt gtcagcatcg caccataagc aagctagctc
     6961 actcgttgag aggaagacga aaatgactcc gtttatgact gaagatttcc tgttagatac
     7021 cgaatttgcc cgccgtctgt atcacgacta cgcaaaagac cagccgattt tcgattacca
     7081 ttgccatttg ccgccgcagc agattgcgga agactatcgt tttaaaaacc tgtatgacat
     7141 ctggctgaaa ggcgatcact acaaatggcg cgctatgcgt accaacggtg tggccgagcg
     7201 tctgtgtacc ggtgatgcat ctgaccgtga aaaatttgac gcctgggcgg cgactgttcc
     7261 gcacactatc ggcaacccgt tataccactg gacgcacctt gaactgcgtc gtccgtttgg
     7321 tatcactggc aaattgcttt ctccgtcaac tgccgatgaa atctggaacg aatgtaacga
     7381 actgctggcg caggataact tctccgcgcg cggcatcatg cagcagatga acgtgaaaat
     7441 ggtcggcacc accgatgacc cgatcgattc tctggagcat cacgcagaga tcgccaaaga
     7501 cggctctttc accatcaaag tgctgccgag ctggcgtccg gacaaagcct tcaacatcga
     7561 acaggcgacc tttaacgact acatggcgaa gctgggcgaa gtttccgata ccgacattcg
     7621 ccgctttgct gacctgcaaa ctgccttgac caaacgtctg gatcacttcg ccgctcacgg
     7681 ctgtaaagtg tctgaccatg cgctggacgt ggtgatgttt gctgaagcga acgaagcgga
     7741 actggacagc attctggcgc gccgtctggc tggcgaaacc ctgagcgagc acgaagtggc
     7801 acagttcaaa actgcggtac tggtgttcct tggtgccgaa tatgcacgtc gcggctgggt
     7861 acagcagtac cacattggcg cgctgcgtaa taacaacctg cgtcagttca aactgctggg
     7921 gccggatgta ggctttgact ccatcaacga ccgtccgatg gcggaagagt tgtctaagct
     7981 gctgagcaag cagaacgaag aaaacctgct gccgaaaacc attctctact gcctgaaccc
     8041 gcgcgataac gaagtgctgg gcaccatgat cggtaacttc cagggcgaag gtatgccggg
     8101 caaaatgcag ttcggttccg gctggtggtt taacgatcag aaagacggta tggaacgtca
     8161 gatgacccaa ctggcgcagc tcggtctgct gagccgcttt gtcggtatgc tgactgacag
     8221 ccgtagcttc ctgtcataca cccgtcacga atacttccgc cgcattctgt gccagatgat
     8281 cggtcgctgg gtggaagcgg gcgaagcacc ggcggacatc aacctgctgg gcgagatggt
     8341 gaaaaatatt tgctttaaca atgcgcgtga ctacttcgcc attgaactga actaaggtct
     8401 ggggttgata tgcaatacat caagatccat gcgctggata acgtcgcggt cgctttagca
     8461 gatttggctg aaggcacaga agtcagtgtc gataaccaga ctgttacgct gcgccaggat
     8521 gttgctcgtg gacataaatt tgcgttaacg gatatcgcaa aaggggccaa cgtcatcaaa
     8581 tatggcctgc cgattggtta tgcattggcg gatattgcgg cgggggaaca cgtccacgcc
     8641 cacaatacgc gcacgaatct gagcgatctg gatcagtatc gctatcaacc tgattttcag
     8701 gatctgcctg cgcaagcggc agatcgtgaa gtgcagatct atcgtcgcgc taacggcgat
     8761 gtcggggtgc gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg
     8821 cggcaaatcc agaaccgttt cctgaaagag accaacaacg ccgaaggtac cgacggcgtg
     8881 ttcctcttca gccacaccta cggctgctca caactgggcg acgatcacat caacacccgc
     8941 accatgctgc aaaacatggt gcgccacccg aacgcgggcg cagtgctggt gattggtctg
     9001 ggctgtgaaa acaaccaggt tgccgcattc cgtgaaacgt tgggcgatat cgatcctgaa
     9061 cgcgttcatt tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat
     9121 ttgcatcagc tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa
     9181 ctgaagtttg gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg
     9241 atgctggggc gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa
     9301 gtgccggaga tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg
     9361 tttgaaaaac tggtcaccat ggtcaacgac tttaaacagt actttattgc ccatgatcag
     9421 ccgatctacg aaaacccatc accggggaac aaagcgggcg gtatcaccac gctggaagac
     9481 aaatcacttg gctgtaccca gaaagcgggt tccagtgtcg tggttgacgt gctgcgttac
     9541 ggcgagcgtc tgaaaacgcc tgggctgaac ttgttaagtg cgccgggtaa cgatgccgta
     9601 gcgaccagcg ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgcggc
     9661 acgccatacg gtggatttgt gccgacggtg aaaatcgcca ccaacagtga actggcggcg
     9721 aagaaaaaac actggatcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg
     9781 cagttgctgg aagaatttat cgacaccatc gttgagtttg ccaacggtaa gcaaacttgc
     9841 aacgagcgta acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt
     9901 cgaaatgagt tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca
     9961 tacctgataa ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtgtcatcgc
    10021 ctttctgatt ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct
    10081 ttcggtctat agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg
    10141 tgccagtgcc atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg
    10201 ggtaatggcg atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc
    10261 tgtcgaacta ttgccggtta tcggtacgat tgtcagtacc tgggcgctgt tccgctgtaa
    10321 agggctcacc atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt
    10381 ctgggcgggg tcgataggcg gcacaatgat tgagggtagt tttctgctta tgaatggctt
    10441 gaatatcatt cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa
    10501 aacccctccc gccgtagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc
    10561 agacgatcgt cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc
    10621 agcgcggttt cgcaagagtc ctgcaatacg ccgatgataa agccgacggc aaccacctgc
    10681 atggcgatat cgttcgaaat accgaacata ttacaggcca gcgggatcag cagcagagaa
    10741 ccccccgcca cgccggatgc gccacaggca cacagagaag ccaccacgct caacagcagc
    10801 gccgtgggca gatcgaccgg gatacccagc gtattaaccg cagccagcgt caacacggta
    10861 atggtgattg ctgcgcccgc catattgatg gtggcaccca gcggaataga aacggaatag
    10921 gtatcgcgat ccagattcag cttttcacac agtgccatat tcaccggaat gttcgccgca
    10981 gagctgcggg tgaagaaggc atacacgccg ctttcgcgca ggcacagcag caccagcggg
    11041 aacgggttac gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt
    11101 aacatacagc caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg
    11161 gtggttgcca gggtagaaga aaccagacca aaaataccaa tcggtgcgaa gcgaatgacc
    11221 agtttcacca taaaggtgac ggcattcgac atatcgttga ccaggttttt ggtggtctcg
    11281 ttaccgtgac gcagtgcgaa accgaggcca attgcccaca ccaggatccc gatgtagtta
    11341 cctttcagca gcgcgtcgat ggggttggaa accatgctca ttaccagccc gcgcatcact
    11401 tctacaatgc ctgacggtgg cgaaatatca cccgcgctac tggataagtg cagggtagaa
    11461 gggaaggcga agctgaaaac gactgcggcc agagcagcag agaaggtacc cagcagatag
    11521 aggaacaaaa tagggcggat attggttttc tgcccgtgct ggtggttagc aatagatgcc
    11581 atcaccagca tcaacaccag gatgggggca acggctttca gtgcgccgac gaacaaagta
    11641 cctaacagac caacagcttc cgccgcgggt tttgatatcc atgccagaag aatccccaga
    11701 acaaggccga ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc
    11761 ggtgaacgtt gcgtagtcat ttttcgatcc tttcattgtg ttatcgcaca tccctggtgc
    11821 gttctgtcga cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa
    11881 gttacagctt cgggaagcga aaaatgctgg attttaaaga gaagtcatat tttgaaactt
    11941 aatggttaca tttgtttgac ataaacagag ggaaggactg gctactaagg ctgattagat
    12001 aacatatggg caaaactatt tggaatatga gaacggttgc gctcctttaa tgtgctatgc
    12061 catataaagt cactggcgca gggagcgcgc agggggcggc caatcgccgc cgccccctgc
    12121 acccccaggc tctggcgaac aaaatcgccg ctgcgcgatt ccctcagctt atcccttgcg
    12181 gctaccgggt cgggcgcaag ggaacgtccc tgtaagatgc gccctcgccg cgcatccatg
    12241 cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg acgccaccaa cacccgtgcg
    12301 gtctttttgt aaagagctac aagccgatta cataattaat agctcacctt tgtctggaat
    12361 gagttatctc taggaatggc ttaatataca gcgaatcatt ctccccgctg cttatcatgc
    12421 cgataattca cccaggcgtt gataataaac gtcatcacca gaatgccaaa caccacgccc
    12481 agcgagacgg cgattggaat atggtagaag tcgacaatca gcatcttgat accgataaac
    12541 accagaatca ccgccaggcc atatttgagc atcgagaaac gctctgctac gcccgccagc
    12601 aggaaataca tcgcacgcag gccgaggatc gcaaacaggt ttgaggtcag cacaatgaac
    12661 gggtcagtgg tcacggcgaa gatagccgga atgctatcca cggcgaaaat cacgtcgctc
    12721 aattccacca gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc attcttacgc
    12781 acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc
    12841 accagcggct tgtcgccaat gcctgattca tcttcatggg cgagcgccat cttcacgccg
    12901 gtaaacagca ggaaggcacc gaagatatac aggatccagt cgaactgcga aattagccag
    12961 ctgccagtga agatcatgat ggtacgcaga acaatcgccc cgagcacgcc atacaccagc
    13021 acgcggcgtt gtaatgccgc cggaacagag aaatagctga acaacatcag ccagacaaag
    13081 acgttatcga ccgccagcga tttctcaatc agataacccg tgagaaaggc cagtgcctgt
    13141 ggatcggcaa cggcgcgacc ttcagtttgc accagatacc accagaaagc ggcgttaaac
    13201 agtaacgaca gcgtcaccca gaccagcgac caggccgcag cctgtttcat ggtcatggca
    13261 tgtgccccac gacgcccctg caacaacagg tcgatagcca gcataatggc gacaacaaca
    13321 gcgaatccgc cccatagcaa cggcgtgccg acagtattca tagaagttcc ttacacataa
    13381 aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt atgcatagac ctcgccttcc
    13441 ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg
    13501 gtgaccggat gtggtttttc acacatcgta atgacgatcg accggcaatg aagttactcc
    13561 cctttgcagg taacaaagta tgacagacca ttcagccagt caatggtctg ttacatacct
    13621 tttacactgt tttactttgg caattatagt tttacgctat ctgccggaaa aatcacccca
    13681 gtctggcggc ggatctcagt cagcagtttg gcggtgatgc gactgaccgc cagccctgga
    13741 tgatccacca gatgctcatc caccagggta gcaaacagct ctgcttcgta gagcatggta
    13801 ttaatatgct gcggctgggt gagatcctgc atctggctgc cacgcggcac gaagcacact
    13861 ttctggcatt cagacagttt ttcaatcacc agcgatcccg cttcgccctg aatctcgctt
    13921 tccaggacag aatcactgac ctttgagtgc tgcaacgtaa cgctgaaatc accgtaatcc
    13981 atcaccacca cgccgtgggc atctacgccg cttgccaaca aactggcggt tgcctgcacg
    14041 cttttcggtt caccgaataa tgccaccgcc gacgccaggc agtaaaagcc gatatccata
    14101 attgaaccgt tagagaatgc cggattaaag gtgttgggat tctcaccatc caggtaacgt
    14161 tgataccgcg aggaatactg gcaatagttg aaaaagactt tacgcaattt gccgactttc
    14221 ggcagcgcct ggcgcagcaa atgaaagttc ggcaggctgg cggttttaaa tgcctcaaac
    14281 agcaccacct gattttcccg cgcacaggca atggcggcat ccacttccgc cagattcgac
    14341 gccagtggtt tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa
    14401 aagtgcaggg cattcgggct ggcaatatac accgcgtcaa tggcatcgct ttccgccatc
    14461 gcttccagcg aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg ctgggcctgt
    14521 tcaaggctgc gggaatatac ggcggttaac ttgtatttac cgctctcatg ggcggcctcg
    14581 acgaactggc gagtgatcca gttcgtacca atcacagcga aacgtatcat aacgcgtgga
    14641 tactccataa agggattctg tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca
    14701 gatacgcatt attctttcgc cggacagcca tgctcactaa acggcccaat catgaccgat
    14761 ggcccgagat atttcggctg gcgatggagg attttggcgt caatcaccgc gctgacacga
    14821 gcgagtttct cccgcagttg agtataaaaa ccgtgcttca tattaaggtc tggcgcgtta
    14881 aacccgatag cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg gttatgaaat
    14941 tcttgcgaga taatggtgat atggttttcc ccaaacacct ttttggcacg caccactgaa
    15001 tcaagcgttg agaatccggc atagtcgcag aagatcactt tcgccggtac gcctttagcg
    15061 atcaaagcct gctgcatacc tgaggcttca tcgtaatttt tacgtccgtt atcgccactg
    15121 actaacagcc atttcacttt tccgacgtgg tacaacgctg ctgcggtatc aatacgtcgg
    15181 gtaaagtagc gattacccgg cctcgccccc agcaataaac ccacgttacg cgccggaact
    15241 gcgttgacat cgctccaggt cagctgcttg ctggcgttga ccattacgcg atcggcgata
    15301 aaaatggtcg ctcctgccac aagtaacagt aagcaggtaa tctttagagt ccggcgagaa
    15361 aaacaaatac ggagaagaag gcggggctgc atggtcgtta tccttaacga aaggggggag
    15421 gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc
    15481 ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct ggtggtactg
    15541 cggctccata tggcagcaca actggtaaaa cgctttgtta tgctctttct ccttcaggtg
    15601 cgccagttcg tgcaccacga tcatgcgtaa aaacggttcc ggcgcgttgc gaaacacagt
    15661 agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcacacgag aaacagcggt
    15721 atgtaatccg agcgcgtttt tcagtacgtg gatcttattg tcatacatca ctttagtgat
    15781 cggcggggca ttgcgcagaa actgattttt tagatcctgg gtatattgcc agagggcttt
    15841 atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg
    15901 ttcgttaatc agcgttcgca cctgggaaag tagctgctcg ggataaccct gaagataagt
    15961 aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca
    16021 ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc
    16081 cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg
    16141 gaagcggcgg atgaatattt gctgcaacag ttggacgaca cagaaatccg cggcccggtg
    16201 ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac
    16261 agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg cctcaacggg
    16321 attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca acagccgggt
    16381 gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact gcgtgcgctg
    16441 cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac
    16501 acttccacgc tggagctgtt cgagaaagtg ctcggcccga ccaccactac gctggcatgg
    16561 aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc cgctggccga tgcgccgcag
    16621 accgttagct ggaagctgga aggtactgac tggactatcc acaaccatgc gaatgtcttc
    16681 tcccgcaccg ggctggatat tggcgcacgc ttctttatgc aacatctgcc agagaatctc
    16741 gaaggtgaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat
    16801 aaaaaccctc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc
    16861 cgtttgaacg ttgaaaccaa catgccagag gcgttagatc gcagcgagtt tatgatcaac
    16921 aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa cccgccgttt
    16981 caccaacaac atgcgctgac cgataacgtc gcctgggaga tgttccacca tgcccgccgc
    17041 tgcctgaaaa tcaacggcga gctgtatatt gttgccaacc gtcacctgga ttacttccat
    17101 aaactgaaga agattttcgg caactgcacc accatcgcca cgaataataa atttgtggtg
    17161 ctgaaagcag tgaagctggg gcgtcgtcgg taagattggg agttacctgc cggatgcggc
    17221 gttaacgcct tatccggcct acgttcagca cgttactccc aggccggata agacgcggca
    17281 agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa aacaaaaaat
    17341 ccctctcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca
    17401 cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc
    17461 aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa
    17521 aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt
    17581 tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg
    17641 ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt
    17701 ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta
    17761 ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg
    17821 agaaaagcta actgcggtgg caccttttct ggcgggtatt cagaacgagg aacaatacac
    17881 ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga
    17941 tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa
    18001 tgccatggct caagccatgc ctggcggtat agccgtgatt cgtaccctta tggatcaata
    18061 tggtttaacc cttgccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt
    18121 gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat
    18181 ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttaa
    18241 aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc
    18301 cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag
    18361 cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc
    18421 taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata
    18481 acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc
    18541 ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg
    18601 ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct
    18661 gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg
    18721 ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt
    18781 gttgcctacc ggcgctttgt cgcgcagtac attgagataa ctcaatacct tcggatgatc
    18841 gatcccgtcg ataggcggaa cgcgcggcac gatcccactg gcgaggatcg tttcatcgaa
    18901 cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc
    18961 gatcatccgg tggtaatagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt
    19021 ggcgatatta aactgcccgc cgatctcgct gtgggcatca aacaatgtta cctgatgccc
    19081 acgcgccgcc gcgttaatgg caaacgccag cccagcaggt cccgcaccga ccaccgccag
    19141 attttttttc tgcacggcag gaaggattgg cattttggtt tcgtggcagg cgcgaggatt
    19201 caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct gattgcagcc
    19261 aatgcaggtg ttgatctcat cggctcgccc cgattgcgct tttgacagca gttccgcatc
    19321 agcaagaaac ggtcgtgcca tcgataccat atcggcatcg ccgcgcgaga gaatatcgtc
    19381 ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga cgtggccttt
    19441 cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcaa tggtcggaat
    19501 acgtgcttca tgccagccaa tgccggtgtt gataatggtc gcgcccgccg cttcaatggc
    19561 ctgcgccagc tctaccgttt cggcaaaagt cccgccgtct tcgaccaggt cgagcatcga
    19621 cagtcggtag ataataatga agtcgttgcc gacgcgttcg cgcaccgcac gcactacttc
    19681 tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt
    19741 gcgcagcgtc agaaattcgt tgatcaaata cccttcggac cccattacct ctacaccgtc
    19801 gtatcctgcc tcccgtgcca gttgcgcgca gtgggcgaaa tcgtcgatca gttgcaggat
    19861 ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggaggg
    19921 agcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggctatttt
    19981 gccgccttcc tgatgtaccg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc
    20041 gttgagcatt gcgccacctt ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct
    20101 gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgctgcca gccgctcggc
    20161 accgtccggg tattcctcca gcccggtgtg cattgagccc atcaacacgc ggttttttaa
    20221 cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag
    20281 tatgtaaaat aattgttatg tggtcggatg agttctaatt tagccgtcga tggagaaaag
    20341 ggaaaagggg agtgcggggt ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc
    20401 cggatgcaac gcttgccgcg ttttatccgg cctgggggat tgcacatgta gggcggataa
    20461 ggcgttcacg ccgcatccgc cagccgttgc aaattactgc ttacggaaaa aatcgttata
    20521 caacatatac aaatgagcgg cactccagga gaaatttggt gcgccttgct gtgcgccagt
    20581 cagcgggttg taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa
    20641 gaacgtatcc gccagcttca gggcatcatc acgataaccg taacgctcca tccctttcag
    20701 accaaaccag aactgatcca cccatacgcg cccacgccag tagatatcag cgccaaaagc
    20761 cggattggtt aacgccgccg ttcccagcgg gacaaaggtg ttgaactctt taggatcgag
    20821 catcaccttc accaccgcgt cggcgttggc ctgcgttgcc gcaccgttaa acagcggcga
    20881 ccagccttcc ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag
    20941 tggtttatct tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt
    21001 gttgatgtag tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt
    21061 accgaggatg gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg
    21121 atccaccgac tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc
    21181 ggcgaatttc accgtccagt cgctacgttt gccgccatta gcgacatatt tatccagctg
    21241 ttctttgtcg ataaacccaa agacggcggc atcatcacgc cccgattccc acgatgcagc
    21301 aacctgtgcc ggaatttcca gactgtcgta caggcctttc tccaccacgc gggcgtagtt
    21361 gttcagccca gactgcgtct cttctttatc gccttttttc accgtaaaca gcatctcgcc
    21421 gctctcagtg ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgtcgtt
    21481 gtgatcgcgg ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc
    21541 caaccaggct ttatcctggg tgacgttgta cacttccatc accgaccagg cggcaaggct
    21601 gggtttggta ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt
    21661 ccacgctatc aggtcgggga caaagcccac atcctgcgga cgcacgctat cgccaggctg
    21721 gatctgccag gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc
    21781 catcgcaaac gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg
    21841 cccggtcacc gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agagccagtt
    21901 accgttgagc gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg
    21961 attggttaac cctttcttca ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa
    22021 cgccggacgc gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt
    22081 cagcagatgg gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt
    22141 aaagcgatta ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga
    22201 ttcgccggag gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc
    22261 atcacgggtg gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc
    22321 ggaaagcggt ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag
    22381 atccagcggt ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa
    22441 gcgcagagtc atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg
    22501 aatactgtat gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg
    22561 gtcgaaattg ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa
    22621 gccgcccatg gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa
    22681 aaacggatta aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc
    22741 agtacggtta atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat
    22801 cagcagagcg caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt
    22861 attaccagta gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt
    22921 tcatgtagaa gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga
    22981 taccaaactg catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt
    23041 aggtgtaagc ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca
    23101 tcccactttc acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc
    23161 gagcggcaaa gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga
    23221 tttccagacc gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca
    23281 tcattgccgg ataggtgtat ttgtcgtcat agcgatcgta tttgccaaaa tgtagccagg
    23341 ctttgctttc atcatgatgt ccggcaggag tcgcggtgac gctataacga aagttgccgc
    23401 ttaagttctg taccttcagc gcagtcataa tgtcgcgggt attgggaatg acgtagccca
    23461 gatcgggggt gaaatcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac
    23521 tgagcatgat ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc
    23581 cctcgaaacc accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca
    23641 cggtccagcc ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac
    23701 cttcaatata ggtaccgtcg ctgtatttat gtttattctc accctctaaa tccatatatc
    23761 caccaccccc taatccctaa ttcgccgtaa aaacgaaatg aaggagaaga tgtcgaggta
    23821 attgcgggtg tttttgtgtc gtcatgttct gccggacgtg cttcttctcc tgccgcagaa
    23881 aaggcgtagc aaaacggcag agcaagtaat gctctgcatg gtgcagtgat taatttcatt
    23941 gtgttaatcc atgagctaaa gaaaaagtac agccttttat cgttgtaatt ataaaaatat
    24001 aaaggcagaa actattttcc ctctcctcat ccaggaagag ggaaaatgcc tgctcatttt
    24061 ttattattct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc gctcttcagc
    24121 cgttaatccc tttatatatt tactgtattt ccaccacgca aagccaagga agataacaat
    24181 accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga atgtcgaagc
    24241 gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac cggtgcggcg
    24301 ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat tcaggtaagc
    24361 cagcatgatg aataacggcg gaagcatgga cgctg
//
LOCUS       DAZPJP010000047        23767 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPJP010000047 DAZPJP010000000
VERSION     DAZPJP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23767
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.047"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..3880)
                     /gene="entF"
                     /locus_tag="ACHU9J_001727"
     CDS             complement(<1..3880)
                     /gene="entF"
                     /locus_tag="ACHU9J_001727"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1905291.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3877..4095)
                     /gene="ybdZ"
                     /locus_tag="ACHU9J_001728"
     CDS             complement(3877..4095)
                     /gene="ybdZ"
                     /locus_tag="ACHU9J_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1905292.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4098..5300)
                     /gene="fes"
                     /locus_tag="ACHU9J_001729"
     CDS             complement(4098..5300)
                     /gene="fes"
                     /locus_tag="ACHU9J_001729"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1905293.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5543..7783
                     /gene="fepA"
                     /locus_tag="ACHU9J_001730"
     CDS             5543..7783
                     /gene="fepA"
                     /locus_tag="ACHU9J_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1905294.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7958..8578
                     /gene="entD"
                     /locus_tag="ACHU9J_001731"
     CDS             7958..8578
                     /gene="entD"
                     /locus_tag="ACHU9J_001731"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1905295.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8700..8852)
                     /gene="hokE"
                     /locus_tag="ACHU9J_001732"
     CDS             complement(8700..8852)
                     /gene="hokE"
                     /locus_tag="ACHU9J_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1905296.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9203..9355)
                     /gene="hokE"
                     /locus_tag="ACHU9J_001733"
     CDS             complement(9203..9355)
                     /gene="hokE"
                     /locus_tag="ACHU9J_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1905297.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9324..9592
                     /locus_tag="ACHU9J_001734"
                     /pseudo
     CDS             9324..9592
                     /locus_tag="ACHU9J_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9795..10913
                     /locus_tag="ACHU9J_001735"
     CDS             9795..10913
                     /locus_tag="ACHU9J_001735"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1905298.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            10979..11227
                     /locus_tag="ACHU9J_001736"
                     /pseudo
     CDS             10979..11227
                     /locus_tag="ACHU9J_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11292..11660
                     /locus_tag="ACHU9J_001737"
     CDS             11292..11660
                     /locus_tag="ACHU9J_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1905299.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11754..12407
                     /gene="nfsB"
                     /locus_tag="ACHU9J_001738"
     CDS             11754..12407
                     /gene="nfsB"
                     /locus_tag="ACHU9J_001738"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1905300.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12515..13762
                     /gene="ybdG"
                     /locus_tag="ACHU9J_001739"
     CDS             12515..13762
                     /gene="ybdG"
                     /locus_tag="ACHU9J_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1905301.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13830..15206)
                     /gene="pheP"
                     /locus_tag="ACHU9J_001740"
     CDS             complement(13830..15206)
                     /gene="pheP"
                     /locus_tag="ACHU9J_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1905302.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15308..18451)
                     /gene="cusA"
                     /locus_tag="ACHU9J_001741"
     CDS             complement(15308..18451)
                     /gene="cusA"
                     /locus_tag="ACHU9J_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1905303.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTTLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18463..19686)
                     /gene="cusB"
                     /locus_tag="ACHU9J_001742"
     CDS             complement(18463..19686)
                     /gene="cusB"
                     /locus_tag="ACHU9J_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1905304.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19702..20034)
                     /gene="cusF"
                     /locus_tag="ACHU9J_001743"
     CDS             complement(19702..20034)
                     /gene="cusF"
                     /locus_tag="ACHU9J_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1905305.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20058..21440)
                     /gene="cusC"
                     /locus_tag="ACHU9J_001744"
     CDS             complement(20058..21440)
                     /gene="cusC"
                     /locus_tag="ACHU9J_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1905306.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21597..22280
                     /gene="cusR"
                     /locus_tag="ACHU9J_001745"
     CDS             21597..22280
                     /gene="cusR"
                     /locus_tag="ACHU9J_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1905307.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22270..23718
                     /gene="cusS"
                     /locus_tag="ACHU9J_001746"
     CDS             22270..23718
                     /gene="cusS"
                     /locus_tag="ACHU9J_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1905308.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5729 a         6490 c         6038 g         5510 t
ORIGIN      
        1 acctgtttag cgttgcgcga ataatcggcc caatttttac aaacgcccct ggagagataa
       61 tatccacatg cgcacaatcc tgacgataga tatccagctc cgcaatccac ggcgaccagg
      121 cgcgttcagg actcatacct tcctgaagcg tgcgttcagc aacaaacaac gtcgctttac
      181 cgtcaaacgg tacgctatga gcagtcgtca gcaggcgcac agcatcagcg tagttgcctt
      241 caatggtggt aaacaactcc gttgaagtac ttccctgctg tgccgccagg aaggcctcgc
      301 gctcgcggtt aatctccgcc agcacttccg ggtccagacc attagcttct ttttcctgcc
      361 agttttgcgt ttctggcggc caggtatcca gcaagccaag aaatgccacc tgttcgccac
      421 gggcacgcag tcgcgccgca ataccctgcg ccagcgtacc gccaagggaa taccccagca
      481 ggtaataagg gccgtgcggt tgttgctcaa gtaacgttgc cagatgcgct tcgcagactt
      541 catccaggtt tgtcgctgtc tgcatgggtc catgagggcg cggcgactga atgccaataa
      601 tcgaccattg tggatcgaga taacgcgaga gtacgctaaa ctgccaggca aaaccggacg
      661 ccggatggaa acaaaacagc gtcgggccat taccttcacg caacggcaga atggtttcga
      721 atcccatgcg ccgggagctg tcctcttcac catcaataat cgttgccagt ttggcgacgg
      781 ttgacgcgac catcacctgc cccggcgtca cctgacgagc aaactgtcga cttaactgcg
      841 ctgccagttt cattgccagt agcgaatgac cgcccagcgc aaagaaatca gcatcggcat
      901 cctgcacgtc acaacccagc aacgacgcga atgccgcggc gataatcgtt tcactgcccg
      961 ctttcggtgc acgtcccgac gcttgtgtct tcagttcagg caacggtaag gctttgcgat
     1021 ccagcttgcc gttggcgcta agaggtaact gtggaagttg cagcagaacc accggtacca
     1081 tatgtggtgg caatgtttca cgaagctgcg cctgcaatgc gctggtatcc aacggcaggc
     1141 ccgattgcga caccagatag cccaccaact gacgcgcatc accaccagtg gctgccgcct
     1201 ggttaatcac acaggcgtgg gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc
     1261 gatcgatttc gcccagttcg atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc
     1321 cgaggtactc caccgcgccg ttatccagcc agcgggcaac gtctccggta cggtacatcc
     1381 gttcgccagg agcaaaagga tcggcaataa agcggctggc ggtcagatcg gggcgtccga
     1441 gatagccctg cgccagttga atgccggtaa gatagagatc acccgccaca cccggcggca
     1501 ccggatgcat catcgcatca agaatacgca gccccatgtt ccacaccgga taaccaatcg
     1561 gcacactgct gccgcgcacc tgtgccagtt cctcgccaaa agccggatac cagctgacat
     1621 ctaccgccgc ttccgtcggg ccatacagat tatgtaacgg cgcaccggtt aactgttgcc
     1681 attcacggca taaatcagcc ggtaaggctt caccactaca gaaaacctgt ttcaacgtcg
     1741 cgcaactctg gcgagcggtt tgcggagtca gcgaggcaac aaatgccgcc agcatcgacg
     1801 gcacaaagtg cgtggtcgtt acgccatatt cggcaaagaa ttgctgcata gcgagcgggt
     1861 cacggtgcgc ttccggttca gccatcacca gttttgcccc tgcgataaac ggccagaaaa
     1921 actcccacac cgagacatca aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg
     1981 taagcgggta gtgattttgc atccataaca ggcggttaac gatagccgtc tgcccgacca
     2041 ttaccccttt cggcctgccg gtggagccag aggtaaagat gatataagcc gtgtggtgcg
     2101 gttgtgaaag ttgcagcggc gcactgccct ggggggtaag cggggcgtta tagcaaaggc
     2161 ttgttaaatt gggaacatcg ctaaagcgcg gcagttgatc gtcggtggta attaacagcg
     2221 acggacgcgc atcttccagc atcattttca ggcgatcgtc cggatagccg gtatccagcg
     2281 gtagccaggc cgcgccagct tcaactattg catggagtgc cagggtcaaa aagaccgagc
     2341 gcggtagtgc caccgccacg ctgtcccctg gtttaacgcc gcgctcacgc agcagattcg
     2401 ccagcgccac cacctgctcg cgcatttccc gatagctgaa caggtaacgc gcatctgcca
     2461 gcgccggagc atccggtgtt tttgccgctt gttctgccac cagcgcgctc agcgtggttt
     2521 ctggaatctc aacctgagtg gcgttgatct gcgccagctg cgcatactca cctggcagca
     2581 taatatcgac atcgccgcac aacagcgccg gatccgcggc gaactgggca atcagcattt
     2641 tcaggcgttc agcatgctgg attaacgttg gctcatcgta acgctgttta ttggcgagga
     2701 tctcaatact caaatcaccg tgttcatccg ggaacagggc cagttcaagg tcattaaccg
     2761 gaccggttgc cagggtatgg gtttgcgcct gaacaccagg aatatccagt tggtaatcaa
     2821 ataccttaat attgagtacc ggaccaaaca gcggttcatc acctgccgct cgcccgctgt
     2881 cacggacaat ttgttcggca tcgtaacgtt gatggcgacg catttttttc agttgagccg
     2941 ccagtcgggt tgccagttcc ggcagcgttt cttgcgccgc aatgtgaata cccaacggta
     3001 aaacgttgag cacgggtccg ctagccgtca gcgccgccga gcccagtcga cgcataaaga
     3061 taaatccggc ggcatagtcc atgcgattgc acaatcgccc cagccacaag gctgccagcg
     3121 caagggctaa atcggtacgc tgcacacctg aaagttgcgt agccagctgg cggaattccc
     3181 cgtcggtaaa ttccagtttc aggcgcagaa tatctgccga agcgctgcgc cccggtaaag
     3241 gtgccggaga aagtgacgcg ggcggcggca gttgacgacg ctgttctgcc cagaatgccg
     3301 catcacgctg ccaggcttcg ctttcgcggt attgctggta ctcttccact acatcagcga
     3361 aaggcgtaaa cggcgaagca ggcgttggtt cgccacgcag ccatgtgcag taaatattgg
     3421 cgatctggcg agtaatggcc gggaaactga agccatcgac cagcaaatgg tgataacgct
     3481 gataccagta ccagcggtta tccgcaacat gtatcagctg atgaaagacc agtggtttac
     3541 cgctatcgac gcgcaaatct tgttgcaaat ccgtcagcat taatgcctgc gcagtaccgt
     3601 gcggatcaat attggttcgc aggtcgataa tttctggctg ttcgaatatc agcgcatcat
     3661 cgacccactg ccagacttcg ccgttatctt ccgtaaaacg catccgcaac gtatctgctt
     3721 gcgctagtcc ggcaaccacc gcgcgggcca gtaatggcgc atcaacatct ccagttaact
     3781 caacgtaatg cgccacgctc caggcggagg gtaaatccga cagtttttct gccatccaga
     3841 tgccgggctg tgcggcgacc aaaggtaaat gctggctcat tgtgcctcct gcaactgggt
     3901 gaaattggcc ggtgtcagag tacgccagtg gacttccagc cactgctggc aggacgcctg
     3961 tgactgcggc tgacacacaa tgtcccagcc tgccgataag aggcattgtt gcggccacag
     4021 actgaattgc ccctgcgcat tgcgcaaaat gtaaaacgct ccctgcggat catcgaaggg
     4081 attactgaat gccatactca actcctgtca tggaaaagtg gttgccagag gtcgattaac
     4141 ccctgcatca agccaccgcg ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag
     4201 aaaatggatt cttttatagg gtgcaattgc gcatacagcg cctgattggc ccgcatgatc
     4261 atcggctcgc gaatacccgc ttccagcaca atgcgcagac cttcggcgct aacttcacca
     4321 gcttttagct tttcaagtaa cacgccctct tgctgcccgt cccgatgcgg ccaccagtac
     4381 gatcctgact ggcttaatac acagccaaag cgttcaggcc agtgcagtcc ggcatacagc
     4441 gcggacagcc caccaaaact ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa
     4501 ggggcaatag ctttcaccag gggtaataac tcttgctgta ctgccagcca gaaatccgca
     4561 ttacacggca gttcgtgggc gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg
     4621 gcgggaggaa gttgctgacg atgggtcagc gaagtcagca caggccagac gggcatactt
     4681 tgcgcccaaa attcgccatc gagcaaaact gccagcggac gttcttctgc tgttacatcg
     4741 ccggtggtaa aaatccatac acgacgtgaa tttttcaacc gttcactttt ccagataatt
     4801 tctttggctg ctatttctgg cacttgcgga caatcccatc ccggttgcag aggcgcttgc
     4861 ggcatttcga gtgcagaaac agcgtgccct cgcccgcctt tccagctttg tggattcagc
     4921 ggatcggcta ttgcctgggg taatagtttt cgccagcctt cacgtaattc gaggcgatcg
     4981 ggggatggtg cagaaaaaat gtcatcgcgt tcggtgggaa taaagcagta gctgccgcgc
     5041 cagttggcat tgagttgtgt cgtccactgc cagacatcag tacctgcaat tcgctgcatc
     5101 gactggggct ggctgttctg atgatgatcg gtcacaccag tgatgtagat ccatacgcgc
     5161 tttatcgtcg agtattcttc agaaccttgg ggatcacgcc accagaaagt gacctcaaac
     5221 atttcgtcat tcagacgctg ccattccggg ccatgtttcg actgccacca gctctcactt
     5281 cctactttta acgccgtcac accataaccc catgtttact gtgcaatttt tcattgattg
     5341 cagaaatata ttgataatat tattgataac catttgcatt tgcaatagcg taatggcgcg
     5401 ccgtgggaag cgcggacatt tattaaccaa ctgcactgcg tgtctttcag gatcaaaggt
     5461 tttcgcggta gcgggatgcg tcgtgttgag gacgaccatg cccgacagtt gcaattcgtg
     5521 gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct tgttggtcaa
     5581 tctggggatt tatggggtag cgcaggcaca agagccgacc gatactcctg tttcacatga
     5641 cgataccatt gtcgttaccg ccgccgagca gaacttacag gcgcctggcg tttcgaccat
     5701 caccgcagat gaaatccgca aaaaccccgt tgcccgcgat gtgtcggaga tcatccgtac
     5761 catgccaggc gttaacctga ccggtaactc caccagtggt cagcgtggga ataaccgaca
     5821 gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg gcaagccggt
     5881 aagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc gtggtgatac
     5941 ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc
     6001 gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg
     6061 tgagtggcac ggctcctggg acgcttattt caatgcacca gaacataaag aggaaggtgc
     6121 cactaaacgc accaacttca gcctgaccgg tccgctgggc gacgaattca gcttccgctt
     6181 gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg gccatcagtc
     6241 cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg tgatcaacaa
     6301 agatattaat ggcgtggtgc gctgggattt cgcgccattg caatcgctgg aactggaagc
     6361 aggttacagc cgccagggca acctgtatgc gggcgacacc cagaacacca actccgattc
     6421 ctatacccgc tcgaaatatg gcgatgaaac caaccgtctg tatcgccaga actactcgct
     6481 gacctggaac ggcggctggg ataacggagt gaccaccagc aactgggtgc agtatgaaca
     6541 cacccgtaac tcgcgtattc cggaaggtct ggcaggcggt accgaaggga aatttaacga
     6601 aaaagcgaca caggatttcg tcgatatcga tcttgatgac gtgatgctgc acagcgaagt
     6661 taacctgccg attgatttcc tcgttaacca aacgctgacg ctgggtacgg agtggaatca
     6721 gcaacggatg aaggacttga gttccaatac ccaggcgctg accggggcga acaccggcgg
     6781 cgctattgat ggtgtgagtg ccaccgaccg tagcccgtat tcaaaagcag aaattttctc
     6841 gctgtttgcc gaaaacaaca tggagctgac tgacagcacc atcgtaacgc cggggctgcg
     6901 tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg
     6961 tttaggcgat gacttcacgc tcaaaatggg cattgcccgt gcttataaag caccgagcct
     7021 gtaccagact aacccgaact acattctcta cagtaaaggt cagggctgct atgccagcgc
     7081 gggcggctgc tatcagcaag gtaacgatga cctgaaagca gaaaccagca tcaacaaaga
     7141 gattggtctg gagttcaaac gcgacggctg gctggcgggc gtcacctggt tccgtaacga
     7201 ttatcgcaat aagattgaag cgggctatgt ggctgtaggg caaaacgcag tcggcaccga
     7261 tctctatcag tgggataacg tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa
     7321 cgtaccagtt agcgaaatgg tgatgtggac caataacatc acttatatgc tgaagagtga
     7381 aaacaaaacc acgggcgacc gtttgtcgat catcccggag tacacgttga actcaacgct
     7441 gagctggcag gcgcgggaag atttgtcgat gcaaacgacc ttcacctggt acggtaagca
     7501 gcagccgaaa aaatacaact acaaaggtca gccagcggtt ggcccggaaa ccaaagaaat
     7561 cagtccttac agcattgtgg gtctgagcgc gacctgggat gtgacgaaga atgtcagtct
     7621 gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac
     7681 cacgggcgat ctggcagggg ccaactatat cgcgggtgcc ggggcgtata cctataacga
     7741 gccgggacgt acgtggtaca tgagcgtaaa cactcacttc tgatgctaac gtcagattgt
     7801 tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
     7861 atcttgccaa ttcaatatat tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc
     7921 agttttgcgt ttgtcatcag tctcgaatat ggtcgatatg aaaactacgc atacctccct
     7981 cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt tttgtgagca
     8041 ggatttactc tggctgccgc actacgcaca attgcaacac gctggacgta aacgtaaaac
     8101 agagcattta gccggacgga tcgctgctgt ttatgcctta cgggaatatg gctataaatg
     8161 tgtgcccgca atcggcgagc tacgccaacc tgtctggcct gcggaggtat acggcagtat
     8221 tagccactgt gggactacgg cattagccgt ggtatctcgt caaccgattg gcattgatat
     8281 agaagaaatt ttttctgtac aaaccgcaag agaattgaca gacaatatta ttacaccagc
     8341 agaacacgag cgactcgcgg actgtggttt agccttttct ctggcgctga cactggcatt
     8401 ttccgccaaa gagagcgcat ttaaggcatc ggagagacaa actgaagcag gttttctgga
     8461 ttatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga atgagatgtt
     8521 tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac acgattaatt
     8581 gacaacatct ggtacgattc gcccgcagcc atcactgact gcgggcgaaa gtgtaaagca
     8641 ggtgccttac cactctgacc tgacaaccgg atatgcaggg attgctcccc gcacaacggc
     8701 tacttcttcg gttcgtaagc gagaacagcc ttaaactcaa tattacgttc ctttacggtg
     8761 aactcacaca gcgagtctcc gaccagaagc gtaaatccca gcaccgtcaa acacagcact
     8821 atgactgcca caagggcata tttcgtcagc atgtttatat tgcctccatg cgaagaaggg
     8881 actaccatcc gagttgttga ggttcggagt ggcagcccca ggattgatag aaatatctcc
     8941 tggggcttca tctttccaga cctcagagta aacctgaaac cggaaagctt caggcacccg
     9001 ccgttatctt acccttcctc agcaaaatat ctgtttattt atacagtaag tcctgtttcg
     9061 gacttctgtt ggcaacactc gttattgctc gtaaaacgaa atcgcccgca gccatcactg
     9121 accgcgggcg aaagtgtaaa gcaggtgccc taccatcctg acctgacaac cggatatgcg
     9181 gggattactc cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact
     9241 caatattacg ttccttcacc gtaaactcac acagcgagtc tccgaccaga agcgtaaatc
     9301 ccagcaccgt caaacacagc actatgactg ccgcaagggc atatttcgtc agcatgttta
     9361 tattgcctcc atgcgaagaa gggactacca tccaaattgt tcgggtttgg agtggcagcc
     9421 ccagggttga tagaaatatc tcctggggct tcatctttcc agacctcaga gtaaacctga
     9481 aaccggaaag cttcaggcac ccgccgttat cttacccttc ctcaacaaaa tggctgttta
     9541 tttatacagt aaatcctgtt tcggacttct cactcctcat ttaatcaact gaagctgagc
     9601 ccgttttttc tgcatgcctc aatcgtcaaa attcttcgcc atcagccagc tctgccataa
     9661 cttgccacat tgaaaaagta gccaaattgc ttcagtcacc atcatcaaaa aaagaaacat
     9721 tccagaactt ccgcatctcg cccagccgct ccgacttcta tactgaatag aaaacgccaa
     9781 cataagagaa acctatgcca ttacccgatt ttcatgtttc tgaacctttt accctcggta
     9841 ttgaactgga aatgcaggtg gttaatccgc cgggctatga cttaagccag gactcttcaa
     9901 tgctgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg
     9961 aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcaat
    10021 tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg
    10081 gcggtggcac acacccgttt cagaaatggc agcgtcagga ggtatgcgat aacgagcgct
    10141 atcaacgaac gctggaaaac tttggctatc tcatccagca ggcgaccgtt tttggtcagc
    10201 atgtccatgt tggctgcgcc agtggcgatg acgccattta tttgctgcac ggcttgtcac
    10261 ggtttgtgcc gcactttatc gccctttccg ccgcgtcgcc atatatgcaa ggaacggata
    10321 cgcgttttgc ctcctcacga ccgaatattt tttccgcctt tcccgataat ggcccgatgc
    10381 cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgtttgagt tacaccacga
    10441 tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg
    10501 tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtaaat atggcgggat
    10561 taattcaggc taccgcccac tggttactga cagaacgccc gttcaaacat caggagaaag
    10621 attacctgct gtataaattc aaccgtttcc aggcctgtcg ctatgggctg gaaggcgtca
    10681 ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agacaccttg cgattgctgg
    10741 aaaaaatcgc cccttccgca cataaaattg gtgcatcgag cgcgattgag gccctgcatc
    10801 gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggtg
    10861 gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccgatgac taagtaggga
    10921 attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat
    10981 gaaacaccct ttagaaacct tgactaccgc agcaggcatt ttgctgatgg ctttcctctc
    11041 ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg gcacaaaaac tggtgaccat
    11101 gttccatctg atggatctta gctagcttta cactttattg ttttgtctgt ggtttttagt
    11161 gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc
    11221 ggattaaacg tcgattatcg ccgtacttcg cggcatactt tgcttattct ccttcgccta
    11281 aaggaacgtt tatggataag caatcactgc acgaaacggc gaaacgcctg gcccttgagt
    11341 taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg
    11401 gcaaaatttt tatgctgtcg tcggagctgc gcggcgtccc ctttatcaat ctgaagtccg
    11461 atccacaaaa atccctgtta aatcagcaga tatacccgag cattaagcca gggtatcaca
    11521 tgaataaaaa gcactggatt tcggtgtatc ccggcgagga aatctccgaa gcgttacttc
    11581 gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa
    11641 aaagagtgcg tccaggctaa agcggaaatc tatagcgcat ttttctcgct taccatttct
    11701 cgttgaacct tgtaatctgc tggcacgcaa aattactttc acatggagtc tttatggata
    11761 tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagt aaaaaactta
    11821 ccccggaaca ggccgagcag atcaaaactc tcctgcaata cagcccatcc agcaccaact
    11881 cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat
    11941 ccgctgccgg taattatgtg ttcaacgaac gtaaaatact tgatgcctcg cacgtcgtgg
    12001 tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag
    12061 atgctgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt
    12121 tcttcgctga tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg
    12181 tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac
    12241 ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct
    12301 acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc
    12361 tgccgaaatc tcgtctgccg caaaacatca ccttaaccga agtgtaattc tctcttgccg
    12421 ggcatctgcc cggctatttc ccctcagatt ctcctgattt gcataaccct gtttcagccg
    12481 tcatcatagg ctgctgttgt ataaaggaga cgttatgcag gatttaatat cccaggttga
    12541 agatttagcg ggtattgaga tcgatcacac cacctcgatg gtgatgattt tcggtattat
    12601 ttttctgacc gccgtcgtgg tgcatattat tttgcattgg gtggtactgc ggaccttcga
    12661 aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc ataacccaga ataaactctt
    12721 ccaccgttta gcttttaccc tgcaggggat tatcgtcaat attcaggcgg tattctggct
    12781 gcaaaaaggc accgaagcgg cagatattct gactacctgc gcgcagttgt ggatcatgat
    12841 gtatgcgctg ctttcagtct tctcgttgct ggatgttatt ttgaatctgg cgcagaaatt
    12901 cccggcggca tctcagttac cgctgaaagg gatatttcag gggattaaac tgatcggcgc
    12961 gatcctggtc ggcattttga tgatctcgct gctgattggt cagtcgccag cgattctgat
    13021 cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta tttaaagatc cgattcttgg
    13081 tctggtggcc ggtattcagc tttccgcgaa cgatatgctg aaactgggcg actggctgga
    13141 gatgccgaaa tacggcgcag atggcgcggt gatcgatatt gggttaacca ccgtcaaagt
    13201 gcgtaactgg gacaatacca ttaccactat tcccacctgg tctctggttt ctgactcctt
    13261 taaaaactgg agcgggatgt cagcatctgg cgggcgacgt attaagcgca gtatcagtat
    13321 tgatgtcacc agtattcgtt ttcttgatga agacgaaatg caacgtctga ataaagcgca
    13381 tttgttaaag ccttatttaa ccagccgcca tcaggaaatt aatgagtgga atcgccagca
    13441 aggttctacg gagtcggtat taaacctgcg ccgaatgacc aatattggaa cctttcgtgc
    13501 ctatctgaac gaatatctgc gtaaccatcc gcggattcgt aaagatatga ccttaatggt
    13561 acgccaactg gctccaggtg ataacggttt accgctcgag atctacgcgt ttactaacac
    13621 cgtggtgtgg ctggaatatg aaagcattca ggctgatata ttcgatcaca tatttgcgat
    13681 tgtcgaagag tttggtctgc gacttcatca gtcgccaacc ggcaatgata ttcgctctct
    13741 ggcgggtgca tttaagcagt aattaaaaaa accgctctca tcgaatggat gagagcggac
    13801 atcgttttgt cggatgtagc gtgaatgcct tatttccgac gcagcgtttt aaatgccaca
    13861 aacaggaata caatccacac cggcagcagg atcgctgaca agcgcatatc atccatcgtg
    13921 cacatcagca gcaaaatcat gccgaggaag gcaatgcaga gatagtttcc gaacggatag
    13981 agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca tcgctgcacg aaaacgcaga
    14041 tgcgccagac agatcataat ccagttcaac agcagcgttg ctaccaccag cgccatcagc
    14101 agaccaaacg ctttttgcgg cagcagatag ttgattaaca ccaccagcga agtgatcgct
    14161 ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc tgacgcgagt caaaaacttc
    14221 ggcgcattac cctgcacaga aaggccaaac agcatgcggc tgttagagta aaccccgctg
    14281 ttatacactg acagcgatgc taccagaatg acgaagttca gcgcagaagc taccacgttg
    14341 ctgtcgagat tatggaaaat catcacaaac gggctactgt tggatttcac ttccacccac
    14401 ggatagagcg ccagtaaaac caccagtgaa ccgatgtaaa acagcaggat gcgatacacc
    14461 acctgattta ccgcttttgg aatgctttta tccggatcgc gcgcttcagc ggcagtaatc
    14521 ccaatcagct ccagaccgcc gaaggagaac ataattaccg ccagcgacaa aatcagccca
    14581 ttccagccgg tggcgaagaa accaccgtag cgccagaggt tgtcgatact ggctttctcg
    14641 ccgccgtgac cagaaaacag cagccacagg ccaaagccga tcataccgat gattgccagc
    14701 actttaatca acgcaaacca gaactcggtt tcgccatata agcgcacgtt caccaggtta
    14761 acggcgttga tgataataaa gaaggcggca gcccaaatcc acgttggaac atccgggaac
    14821 cagtactgca tatagatgcc cgcagcggtc agctctgcca ttcccaccag cacgaacatt
    14881 acccagtagt tccagccaga gaggaagccc gcaaacggtc cccagtattt ataggcaaag
    14941 tgggcaaatg aaccggatac cggctcttcg accaccatct cgccaagctg gcgcataatc
    15001 aggaaagcga tgatcccggc gacgccgtag cccagcaata cagccggacc cgccatctga
    15061 atcgccggac caatgccaag aaacagacca gtaccaattg cgccacccag cgcaatcagt
    15121 tgaatatgac ggttatgtaa tccgcgatga agcgtcggct cttgattcga cgcagtatct
    15181 tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg tgtctttttt gttgaggggc
    15241 accttttaac atttagtgcc catcgtcgca agacacaatc cacacggtta aacggggtat
    15301 cctgctttta tttccgtacc cgatgtcggt gcagccacat cagcttatac gccgccggga
    15361 taataaacag cgacagcaaa ggtgcggtga tcatgccgcc aatcatcggt gcggcaatcc
    15421 ggctcatcac ctctgaacca gcccccgttc cccacagaat cggcagcaga ccggcgataa
    15481 tcaccgccac cgtcatcgct ttcgggcgca cgcgcagtac cgcgccgtga tacaacgcct
    15541 catccagctt ctgctcactg aatgtttgcg gattattcaa cgacggctcg gcctctatgg
    15601 cgtgacgtaa atacatcagc atcaccacgc caaattcggc ggcgaccccg gcaagggcaa
    15661 taaaaccggt gcccgttgcc acagaaagat gaaagcccat ccaccacagc aaccagatgc
    15721 cgcccaccag cgcaaacggt acgctgctga taatcagcaa cgcttcgccc acccgacgga
    15781 atgccagata caacagcacg aagatgatca tcagtgtcat cggcaccatg agtttcagct
    15841 tatggttggc gcgctccagt agctcgaact gcccggagaa tgccacgctg gtgcccggtt
    15901 ttaactgcac tttttcagct atcgcttttt gcaaatcgtg aaccaccgac accatgtcac
    15961 gatcgcgggc atcgatataa atccagctcg tcgggcgcgc attctcggtt ttcagcatcg
    16021 acggtccggt agagacttta acgtcggcca cgtctgccag ggtgatttgc tgcttcatcg
    16081 gcgtcaggat cggtagctgg cgcagtgcct gcgggctatc gcgccagctt tgcggataac
    16141 gcagattaat gggataacgg gcaatccctt ccaccgtttc gccaaccatc gccccgccca
    16201 ccgcagaagt cacaaacaac tgcacatccg ccaccgtcat accgtaacgc gcggcttttt
    16261 cacggttaat ctcaacgttg atatagcgcc caccttccag acgctcggct agagcagaag
    16321 ccacgcttgg caccgttcgc gctacttctt caatttgctc agccatcgcg tcgatatccg
    16381 ccagcacagt gccggaaact ttaatgccga tggggctttt aatgccggtt gagagcatat
    16441 cgatacggtt acgaattggc ggcacccaca gattcgccag ccccggcagc cgcacggtgt
    16501 tatccagttc ctcaatgatt ttgtccatcg tcatgccagg ccgccactga tcctgcggct
    16561 taagctggat ggtcgtttca accatctcga gcggagcaga atcggtggcg gtttccgctt
    16621 tcccggtttt gccaaatacc cgcgccactt caggtacgct cataattagc ttgtcggttt
    16681 tttgcagcat actcgccgcc tctgctgcgg aaatccccgg cagcgtcgat ggcatataca
    16741 acaagtcgcc ttcattgatc tgcggtaaaa attccccgcc aactttattg agcggccaga
    16801 gaaccgtcag caccgaaagc gctgccacca acagcgtggt tttcggccag tgcagtactt
    16861 tcagcaatag aggatgataa acacgaatca aaaagcgatt gagcgggtta ctgctttccg
    16921 gcggaatttt gccacggatc cagtacccca tcaggatagg gatcactacg atcgccagca
    16981 gcgccgcgcc cgccatcgca taggttttag tgaacgccaa cgggccaaac agacgccctt
    17041 cctgcccttc cagggtgaag atcgggataa acgacaacgt gataatcagc agactgataa
    17101 atagcgccgg acccacttca acagatgcat cggtgatcac ctgccagcgc gttttattat
    17161 ccagcgtggc gtcaggatgc tggtgctgcc actcttccag ccgtttatgt gcattttcga
    17221 tcatgacgat agcggcatcg accatcgccc cgacggcaat cgcaatgccg cccaacgaca
    17281 taatattggc attcagtccc tggaagtgca tgacaataaa agcaatacac aaccccagcg
    17341 gcaacgaaat aatcgccacc agcgccgagc gcacatgcca gagaaacagc gcacagacta
    17401 ccgccaccac gataaactct tccagcaact tgccgctgag gttgtcgatg gcgcggtcaa
    17461 tgagctggct gcgatcgtat gttgtaacta tctccacgcc ttccggcaga ctacttttca
    17521 gcgtttccag tttgtccttc acggaggcga tcacttctcg ggcgtttttg ccggagcgca
    17581 ggatcaccac cccgcccgcc acttcgcctt cgccattcag ttcggcaatg ccccggcgca
    17641 tctccgggcc aatctggacc ttcgcaacat cacgcagata aacgggcacg ccattttcac
    17701 tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg cagatagccg ctggcgcgca
    17761 ccatatattc cgcttccgcc agttcgatcg acgaaccgcc cgcttcctgg tttgaagcat
    17821 ccagcgcgct ttttacttcg gctagactga tgccatattg cgccaggcgc tggggatcga
    17881 taaccacctg atactctttc accacgccgc ccaccgacgc cacttccgca acgtcaggga
    17941 tggtttttag ctcatatttg agaaaccagt cctgcaatga gcgtaaatcg gccagatcgt
    18001 gcttaccgct gcgatccacc agtgcatatt catagatcca gccaacaccc gtggcatctg
    18061 gtcccagctc ggcgctgact cccgcaggca gcttaccctg tacctggttg aggtactcca
    18121 gcacccgcga acgcgcccag tacggatcgg taccatcttc gaaaatgaca tacacataag
    18181 agtctccaaa ctgcgagaaa ccgcgcacag tcttcgcgcc aggcaccgac aacgtggtgg
    18241 tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctgaccg ggatagctgg
    18301 ttttaataat cacctgcaca tcggagagat ccggcagcgc atccactggc gtgttaatga
    18361 tggtccaggt gccccagatg ctcagaaaca acgcgcccat cagcaccaaa aaacggttcg
    18421 ccaccgagcg acgaataatc cattcaatca ttggttattc cctcaatgcg cattggtagc
    18481 actttcagag cgcatccgct ccagtgcgcc agaaatattg gcttcggaat caatcaggaa
    18541 caggccgctg gaaaccacct tttcaccttc cgccagacca gagtgtaacg cggtgacgcc
    18601 ttgcgatgcc tggaaaacgg caacgcgttt cggtacaaag cgtccgtcgg catcaacggt
    18661 aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga gcagcatcgg
    18721 ttcgctggcg gtgttgagtt gcaaccaggc gttcatgccc ggttttagcg cctcgtcggc
    18781 gttgtcgact tccagacgca gctgcagcgt gcgggtcgcg gcatccacgc caggtagcag
    18841 cgtccatttg cggatggtga gtgttttatc cggtcgcgcc ggaacggtga gcgtaaactg
    18901 cgaggcatct ttcaccagcc aggcgataga ctccgggatc gcagcagtga cccacaccgg
    18961 gtccataccc tgaattttcg ctaccacgtt atctttggcg atattcattc ccgcgcgcag
    19021 atcaaacgcg gtgatcacgc catcaatggg tgctttgagc gtaaagcgag tctggatttt
    19081 ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt cccgccagtc gcagccgctc
    19141 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcagcagta aatactcact
    19201 ctgcgcttcc acccagtcag gaatggtcag gtcgagaagc ggtgtgccct tttgtacttt
    19261 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag cctgcacaat
    19321 ggcatactga tactcgttgt aactgacatt cgccgggaaa ctctgggcaa aagtcagcgg
    19381 tccgcgcgtg acggtagccg ttttcacccc cagattctgc gtctgagtcg ggtcaatgcg
    19441 cacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
    19501 cggcgattta cctggtttat cgaaccgcgt attggggtac atcgggtcgt accagaataa
    19561 gattttacgt tctgcggtcg acgttttttc tgcgggtggt tcagcctttg caaaccaggt
    19621 aaaacctgcc gcagaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
    19681 tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata aagaaaggtt
    19741 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcac tcattttcgt
    19801 ctgcggggtg atggtaaagc gcatagtcat ctccggccag ttcacggcag caatcggatc
    19861 gtgatggatg gtgatttttt tgctttccag atcaataccc tttaccacgc cagtggcgct
    19921 aataacctgc ggttgtactt cgctcatggt ttcatgatga tgttcgttag cctgggcatt
    19981 aaagccaata acggtaaaca gactgaacat tgcgacttgc agtgcttttt tcatttattc
    20041 tctcctggag ttaaaaatta ttgctgccaa ccgcccccta aggcggtata caaagaaatt
    20101 tcgttaacct gacgggcata attcaggtcg agtaaagttt gtcgggttgc aaataaagaa
    20161 cgctcggcat ccagcacttc cagataactt accgcgccgt gctgatataa tgcccgcgcc
    20221 cgttgcaaag taatttgcag cgacgccaga taacgctgct gggcgctgat ttgatcgttc
    20281 aggctttgac gtaatgccag cgcatctgcc acttctttaa aggcgttctg gattttctgt
    20341 tcataattca ctaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
    20401 cgtccggcat taaaaatggg gatctcaatt ttaggaataa aattccacat cccgctgcta
    20461 gcgttaaata atgaagacag atcgctgctg gcggttgata ttccgctggt caggctgata
    20521 gacgggaaaa atgccgcacg cgcagcacca atattagcat tagccgccat taacgcgtgt
    20581 tcagcttcca taatatccgg gcgctgcaat aagatttgcg acgacaagcc cgccggtaat
    20641 ttaacgcttt gcaggctgcc gctgtttact gtctgcgctt gcggcagttt gccgtagctt
    20701 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
    20761 tcactgcggg tactttctat caccccgcga gcctgttcca gcgccagaac attgctgcta
    20821 ccggtcaaca gttgtttttc gacaaacgca tatgactgct gataattacg cagcgtttct
    20881 tcggctattt gcaattgcgc atacgccagt tgctgattga aatagctttg cgcgacatta
    20941 gaaaccagca gaatatgcac cgcgcgctga gcttcctcag tggctaaata attttgtcgc
    21001 tcggcttcgc tcatgttctt taagcgaccg aaaaaatcga ggtcaaagct ggcgttaagg
    21061 ccggtcgaaa actcccgtgt cgtggcggta ttccctttaa gattaccgct ccagctgccg
    21121 ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt
    21181 gcctcctgca ctttcagcgt cgccatgcgc aaatcccggt tattcaccag cgcctcgcta
    21241 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccacgtt ctggtagtta
    21301 tctgctgcgt taaccaagcc gttctggctg agtgagaact gctgcggcac gggcattgcc
    21361 gtacgctgat aatccggtgc caatgaacaa ccggttagcg caagggccac acaaaatggc
    21421 agaagtttac aaggagacat aggctcataa tttctggtga ttttataccg ccaactttac
    21481 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
    21541 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaaaatga
    21601 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
    21661 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
    21721 ccggtgatta tgacctgata atcctcgata ttatgctgcc ggacgtgaac ggctgggata
    21781 tcgtgcgcat gttgcgctcc gccaataaag ggatgccgat tctgttgctt accgcgcttg
    21841 gcaccattga acatcgcgtc aaggggctgg agttgggggc cgatgactat ctggtgaagc
    21901 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct cctgcggcgc ggggcggcgg
    21961 tgattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
    22021 tcacccgcag cggcacgcgc atcactctga ccagtaaaga gtttactctg ctggagtttt
    22081 ttctccgcca tcagggcgaa gtgctgcccc gctcgcttat cgcctcgcag gtgtgggaca
    22141 tgaatttcga cagcgacact aacgccattg atgtggcggt gaagcggcta cgcggcaaaa
    22201 tcgacaacga ctttgagccg aagctgattc agaccgtgcg cggcgtgggc tatatgcttg
    22261 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgtctgacc
    22321 ttttttatca gcctggccac catcgcggcg tttttcgcct ttgcatggat catgatccac
    22381 tcagtgaaag tgcattttgc cgagcaggat attaatgatt taaaagaaat tagcgccacc
    22441 cttgaacggg tacttaatca tcctgacgag acgcaagccc gacgcttaat gacgctggaa
    22501 gatatcgtca gtggttattc caacgtgttg atttccctgg cagatagcca cggcaaaacg
    22561 gtgtatcact cccccggtgc gccggatatc cgcgagttta cgcgtgacgc catacccgat
    22621 aaagacgctc agggtggcga ggtgtatctc ctttccggcc cgacgattat gatgccaggc
    22681 cacggtcacg ggcatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
    22741 ttggtggacg gcaaaccgat ttatacgctc tacatcgcgc tttcgatcga ttttcatctt
    22801 cattacataa atgatttgat gaataaactt attatgaccg catctgtcat cagcatcctg
    22861 atcgtcttta tcgtgctatt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
    22921 cgtcaaatcc agaatatcac ctcgaaagat ctcgacgttc gccttgaccc gcagaccgag
    22981 cccattgagc tggaacagct ggtactgtcg ttcaaccata tgatcgagcg tattgaggat
    23041 gtctttaccc gccagtccaa tttctcagcg gatatcgctc acgaaattcg cacgccgatt
    23101 accaatctca tcacgcaaac ggaaatcgcc ctcagccagt ctcgcagcca gaaggagctg
    23161 gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat
    23221 atgctgtttc tcgctcaggc cgataacaac cagctaatcc ccgaaaagaa aatgctcaac
    23281 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgttagcgga agatcgcggc
    23341 gtggagttgc gatttgttgg cgacaaatgt caggttgcgg gcgatccgct gatgctgcgt
    23401 cgggcgttaa gcaacctgct ttctaatgcc ctgcgttata cgccaaccag agagacaatt
    23461 gtagtgcgct gtcagacggt cgatcaccag gtgcaagtta ccgtcgaaaa ccccggtacg
    23521 cccattgcgc ccgagcactt accgcgattg tttgaccgtt tctaccgcgt tgacccttcc
    23581 cgccagcgaa aaggcgaagg tagcggcatt gggctggcga tagtgaaatc gattgttgtc
    23641 gcgcataaag gcacggttgc agtaacgtca gatgcgcggg ggacaaggtt tgtgattatg
    23701 ctgccggaga gagagtgatt attaagttca aattcagggc gtggtgcatc acctcctggt
    23761 attgttg
//
LOCUS       DAZPJP010000048        23515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPJP010000048 DAZPJP010000000
VERSION     DAZPJP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.048"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHU9J_001747"
     CDS             <1..2064
                     /locus_tag="ACHU9J_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1905309.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHU9J_001748"
     CDS             2080..3099
                     /locus_tag="ACHU9J_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1905310.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHU9J_001749"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHU9J_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1905311.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHU9J_001750"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHU9J_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1905312.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHU9J_001751"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHU9J_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1905313.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHU9J_001752"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHU9J_001752"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1905314.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHU9J_001753"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHU9J_001753"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1905315.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHU9J_001754"
     CDS             6828..7646
                     /locus_tag="ACHU9J_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR1905316.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHU9J_001755"
     CDS             7698..8444
                     /locus_tag="ACHU9J_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1905317.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHU9J_001756"
     CDS             complement(8418..9383)
                     /locus_tag="ACHU9J_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1905318.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHU9J_001757"
     CDS             complement(9380..10384)
                     /locus_tag="ACHU9J_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1905319.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHU9J_001758"
     CDS             complement(10381..11658)
                     /locus_tag="ACHU9J_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1905320.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHU9J_001759"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHU9J_001759"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1905321.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="ACHU9J_001760"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="ACHU9J_001760"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1905322.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHU9J_001761"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHU9J_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="ACHU9J_001762"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="ACHU9J_001762"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1905323.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="ACHU9J_001763"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="ACHU9J_001763"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1905324.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="ACHU9J_001764"
     CDS             16207..17562
                     /locus_tag="ACHU9J_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1905325.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="ACHU9J_001765"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="ACHU9J_001765"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1905326.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="ACHU9J_001766"
     CDS             18756..19529
                     /locus_tag="ACHU9J_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1905327.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHU9J_001767"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHU9J_001767"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1905328.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /gene="yegR"
                     /locus_tag="ACHU9J_001768"
     CDS             20916..21233
                     /gene="yegR"
                     /locus_tag="ACHU9J_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1905329.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHU9J_001769"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHU9J_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1905330.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="ACHU9J_001770"
     CDS             complement(23071..23403)
                     /locus_tag="ACHU9J_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1905331.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6062 a         5510 c         6025 g         5918 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcagt
//
LOCUS       DAZPJP010000049        23387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPJP010000049 DAZPJP010000000
VERSION     DAZPJP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.049"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHU9J_001771"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHU9J_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR1905332.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2721)
                     /gene="hyfR"
                     /locus_tag="ACHU9J_001772"
                     /pseudo
     CDS             complement(696..2721)
                     /gene="hyfR"
                     /locus_tag="ACHU9J_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(2751..3164)
                     /gene="hyfJ"
                     /locus_tag="ACHU9J_001773"
     CDS             complement(2751..3164)
                     /gene="hyfJ"
                     /locus_tag="ACHU9J_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1905333.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3157..3915)
                     /gene="hyfI"
                     /locus_tag="ACHU9J_001774"
     CDS             complement(3157..3915)
                     /gene="hyfI"
                     /locus_tag="ACHU9J_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1905334.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3912..4451)
                     /gene="hyfH"
                     /locus_tag="ACHU9J_001775"
     CDS             complement(3912..4451)
                     /gene="hyfH"
                     /locus_tag="ACHU9J_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1905335.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4461..6176)
                     /gene="hyfG"
                     /locus_tag="ACHU9J_001776"
     CDS             complement(4461..6176)
                     /gene="hyfG"
                     /locus_tag="ACHU9J_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1905336.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6166..7746)
                     /gene="hyfF"
                     /locus_tag="ACHU9J_001777"
     CDS             complement(6166..7746)
                     /gene="hyfF"
                     /locus_tag="ACHU9J_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1905337.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7751..8401)
                     /gene="hyfE"
                     /locus_tag="ACHU9J_001778"
     CDS             complement(7751..8401)
                     /gene="hyfE"
                     /locus_tag="ACHU9J_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1905338.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8413..9851)
                     /gene="hyfD"
                     /locus_tag="ACHU9J_001779"
                     /pseudo
     CDS             complement(8413..9851)
                     /gene="hyfD"
                     /locus_tag="ACHU9J_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9868..10815)
                     /gene="hyfC"
                     /locus_tag="ACHU9J_001780"
     CDS             complement(9868..10815)
                     /gene="hyfC"
                     /locus_tag="ACHU9J_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1905339.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10826..12844)
                     /gene="hyfB"
                     /locus_tag="ACHU9J_001781"
     CDS             complement(10826..12844)
                     /gene="hyfB"
                     /locus_tag="ACHU9J_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR1905340.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFITSMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12844..13461)
                     /gene="hyfA"
                     /locus_tag="ACHU9J_001782"
     CDS             complement(12844..13461)
                     /gene="hyfA"
                     /locus_tag="ACHU9J_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1905341.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13714..14184)
                     /gene="bcp"
                     /locus_tag="ACHU9J_001783"
     CDS             complement(13714..14184)
                     /gene="bcp"
                     /locus_tag="ACHU9J_001783"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1905342.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14184..14756)
                     /locus_tag="ACHU9J_001784"
     CDS             complement(14184..14756)
                     /locus_tag="ACHU9J_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1905343.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14902..15780
                     /gene="dapA"
                     /locus_tag="ACHU9J_001785"
     CDS             14902..15780
                     /gene="dapA"
                     /locus_tag="ACHU9J_001785"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1905344.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15797..16831
                     /gene="bamC"
                     /locus_tag="ACHU9J_001786"
     CDS             15797..16831
                     /gene="bamC"
                     /locus_tag="ACHU9J_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1905345.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17044..17757
                     /gene="purC"
                     /locus_tag="ACHU9J_001787"
     CDS             17044..17757
                     /gene="purC"
                     /locus_tag="ACHU9J_001787"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1905346.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17925..18773
                     /locus_tag="ACHU9J_001788"
     CDS             17925..18773
                     /locus_tag="ACHU9J_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1905347.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18788..20803
                     /gene="tmcA"
                     /locus_tag="ACHU9J_001789"
     CDS             18788..20803
                     /gene="tmcA"
                     /locus_tag="ACHU9J_001789"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1905348.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20877..21575
                     /gene="ypfH"
                     /locus_tag="ACHU9J_001790"
     CDS             20877..21575
                     /gene="ypfH"
                     /locus_tag="ACHU9J_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1905349.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21685..21885)
                     /locus_tag="ACHU9J_001791"
     CDS             complement(21685..21885)
                     /locus_tag="ACHU9J_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1905350.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21913..23040)
                     /gene="dapE"
                     /locus_tag="ACHU9J_001792"
     CDS             complement(21913..23040)
                     /gene="dapE"
                     /locus_tag="ACHU9J_001792"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1905351.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23044..>23387)
                     /locus_tag="ACHU9J_001793"
     CDS             complement(23044..>23387)
                     /locus_tag="ACHU9J_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1905352.1"
                     /translation="GIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNGFINEL
                     GWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPMLLGFS
                     DSSYQQFFHEV"
BASE COUNT         5848 a         6221 c         6267 g         5051 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcgtaa taaagacgcc ttaccgtgtt tctgcgctaa
     2641 ccgctccgcg agcattccgt ttaccgcttc aattgttatt ccttgcggcg gggcaaacat
     2701 cgcctcgtct gacatagcca tcaacagtcc ttattaatga gaaatccttc tcacgcaatt
     2761 tttctggcca tcagatagat ggccggttcc tgcacaattt catccaacag atgaatgaat
     2821 tgaatgctac gcgcctgcca cacagacgtc gcgtcatccg ccagtggcgc gaatgcccgg
     2881 gtcaacaggg cggtgtggct ggcgtcaata acaatctcac caaaagtcat cacccccagc
     2941 atcttacgtc gggcttgctc ctcttcgacc agtgcaagcc aatcttcgta ttccgtcagt
     3001 gggcagggga aggcgacatt cagacaatca atcacgccaa cgtgatgacc gatagccagc
     3061 gagtaataca ttacctgaga gctgtgttcc ggcatctcgt cgctactggc gacaaacttt
     3121 tttcgcagcg tccagaaaac aatttccccg cactcttcag tcatgtaacc cccggagtac
     3181 gcgaaagagt tgctgaacga tactattgag acgtggatcg tcggcatcgc gcaaccaggt
     3241 gttaacccga tttcctgtag gatcgtcgct taaatggcgc aggagccgat cgcaaatttc
     3301 tcgcccctga cgatagcccg ccagccgccg cgcttctcgc tcaatggcga tacgctgtga
     3361 tggcgggatc tgcggccaca acggttgcat agtcaccccg gtgggatcgc gataatccac
     3421 cgcgtgaatc ttctgttgca gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc
     3481 cggtgttggc gggcagccgg ggatccaaac atcaatgggg acaatggtgt cgctgccgcc
     3541 ccagacgctg tagagatcgt ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac
     3601 acaaatttta tgatcggggg cagactcata cgcccgaagt gcaggcatac gcatcgcccg
     3661 ggtgactgcg cctgtaaata acaaaatatc ggcgtgacgc ggtgatgaaa taaccttaat
     3721 gccaaaacgt tctgcgtcga atactggtgt gatggcggca aaaatttcga tttcacaggc
     3781 gttgcagccg ccgcaatcga cgcgataaac gtaagccgag cgacggatat cctgtagcaa
     3841 atgctgcttc atcttttgcg tttgctcgtc cagcgtgatg ggctggctga catgttgtgt
     3901 aagcactgga ctcatagctg ctccttagcc accagcggta catctgtatc gtcgttgagc
     3961 agcgtcgcgc gttgtttgca ctccgggcag acgctcgctt gcgcccataa catttcgcgg
     4021 ttttgtggcg cattttgttg ctgtgccaac aattcagcag ccagtgcgac ggttttttgc
     4081 ggggcaaacg ggcgttcgca acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag
     4141 agatcggctt tattggtgac ggtcagttca aagttattgg taagctggat ggctctggtc
     4201 gggcacactt cttcacaacg tccgcagtaa atacaacgcc tcagatagag ctgccaggtg
     4261 cgcgaatttt gctggtcgtc ggtctggata gtcagcgcat ttgccggaca agcacaggcg
     4321 ccgcaggcaa tacattggct gggcatcagg tccggtttcc cgcgaaagcc tgggctgacc
     4381 tccagtggcg cgaagggata tttcaccgtc gcggttccgg cgcgcataat agttttcagt
     4441 aacttcagca tcttctgtcc ttacttcagc ggcgaacggt tacgatcaat gccgtagcgt
     4501 tcgatctctt tatacggcac ggtttttgac tggcgcttgc gcacatctac cagcgtcaca
     4561 cggtcggtac aggagtagca gggatcaagg ctaccgataa tcagcggtgc gtcagaaacg
     4621 gtattgccgc gcagcatgta acgcaacacc ggccagttgg cgtaggtggc ggcacggcag
     4681 cgccagcgga acaatttttg gttatcaccg agcatgctcc agtgcacgtc ttcaccgcgt
     4741 ggcgcttcaa caaagcccag cgcgaatgcg tgaggtttat agctaaagcc ttcggtcagc
     4801 agtggggtat ccggcatgtt gtcgagggca aattccagca ttgccagcga atcaaacgtc
     4861 tctttgacac ggaccatcac gcgggagaaa acatcgccgc cggtaaaggt aaacagtgtt
     4921 tttggaatat tgccgtagtc ggcgtagggg tgatcaaagc gcaaatcacg ggcaaaacca
     4981 ctgccgcgga tcagcggccc tacagggcta taatcacggg cgatttgtcg gtcgagaatg
     5041 ccaatgccct gagtgcgttg ttccatattc ggcgtggcaa gcagcatctc taccagctcc
     5101 gacacgtcgg cgcgcatctc gcgcaccagt ttcagcgttt gcagacgttg ctctttgaga
     5161 atatcgcggc gaacaccacc aatcagattc agaccgtagg ttttacgcga cccggtcagc
     5221 aattccgcca ttgtcatcga cttttcccgc acgcggaaaa attgcataaa gccggtatca
     5281 aatccaacaa aatggcagga gaggccaagg ttgagcaaat gactgtgcag acgttcgact
     5341 tccagcagaa tcgagcgaat agtatgcgct cgttgcggca cctcaatccc cagtgcattt
     5401 tcaaccgagt tggtataggc cacactgtgg gcaaaaccgc aaatcccaca cacgcgatcc
     5461 gataagaagg tcacttcgtt gtagcccatc cgcgtttctg ccagtttctc catgccgcga
     5521 tggacataaa acaggcggta atcagcatcg acaatttgct cgccatccac aaacaagcgg
     5581 aagtgacccg gttcatcgga ggtgatatgc agcgggccaa tagggatcac ccgcgcatcg
     5641 ctattgccct cattgataaa cggatacgtt tcggtatcag tcgtcggttc agggcgcagt
     5701 cgataatcca tcgcatcttt gcgcagcgga tgcatatctt ccggccagtc atcgggcaac
     5761 accaggcgac gctgatccgg caggccaacc ggaatcagcc cgtacatatc gcgaatttct
     5821 cgctcgcccc agaccgcggc agggacgcgc ggtgtgactg acggaaactc ccgactgtcg
     5881 gcatcgacca gtgccttcac cacgatccag catttttcgg ccccttccat tgaaagggca
     5941 taataaaccg cgtaatgacc gttaagtgtc cgctcgtcgt tgccaaacag gactggaagc
     6001 cagccatcat gttgataata aagataatgt acaacgtcag gcagcagatt gattttcacc
     6061 gtaatggtga cctgttcagg cgtttgtcgc tcttcatcca gcaccgcgcc ggggaactgc
     6121 gttttcaggg cggcgagaat cgcttcgcca cgatttgacg atgaattaac gttcactgtg
     6181 tttctccgaa acagatgcgg tgcctgaagg caaaaaatca tgccaggtgc tacgttgtgc
     6241 aggcaggtcg tgcgtccctg agaggactat agtggaagcg cccgccagga tcctgatgac
     6301 aggttgtgga atatgcgttc ccatcgccag catcatgacc agcagaatca ccattggcga
     6361 ggtggtcaac cagccgagat caccccggtt aacggcctgc ggcggtttcg ccattaacac
     6421 ccgcgctgcc atccgtacca ggcccgccag caccagcgtt aacagcaata acagcaggac
     6481 gataatcagc aggtgattac gtgccagtcc ggcggtaatg gtcataaatt cgctaagaaa
     6541 aatgttgaag ggcggcatcc ctgccagcgc cagcgcaccg ccgccaaaca gcacggcggt
     6601 aaatggcatg attttgagca tcccacagac gacgttgaga tcgcgcgtgc cgtacttgag
     6661 cagtacattg ccggaaccgc agaacagcag cgtttttgcc agactgtggt ttaagatgtg
     6721 cagcagcgcg gcaaaaattc ccagcggccc gccaatgcct agcgccaccg cgaccagccc
     6781 catgttctcc acgctggagt acgccagcag acgcttaatg tcccgctgta ccagaatgaa
     6841 aaatgccgcc acggcaaccg acaacatgcc gaagatgagc aacaaccggt tggggaaatc
     6901 gctgccgatg gcttggcaaa taatgatgta atagcgaatc agcaccaaca gcgcgcagtt
     6961 cagcaatacg gcggagagca gggcgctgac cggactcggc gcttcactgt gagcatccgg
     7021 cagccaggcg tgcatgggaa atagcccggt tttggtacca aagccaatta gcacaaacac
     7081 aaaggccaac agcattaatg ttgggtcaag caaggacgat tgcttaagaa cctcgctcca
     7141 gaatatcgcc atttctgcct gcggcataac gctggcggcg ttggcgtata ccagcacggt
     7201 accgaacaga ccaaaagcga caccaacagt acaaataatg atgtacttcc atgctgattc
     7261 cagcgatgaa cgctgaccgt aaatgcctac cagaaacgcc gagcttaagg tggtggcttc
     7321 gatcgccgcc cacatcacaa tcaggttatt gctggtaaca accagcagca tggtgaacaa
     7381 aaacagatgg aagaaaccgt agtaatcgca cagcgtaacg ggtgaaagct cgccgtgtgc
     7441 cacttcatga cgcatgtagc caatcgagta aacaccggtg agaaagccaa tcacaccaag
     7501 aatggcgagg aacaaaccgc ccagaccatc aatatgaagc cacagtcccg cagcgaatat
     7561 ttctcctgca tcagcggcag tttggaccac ccagagtgcc agaatcagca gcagtgtgat
     7621 ccctaagcta tgtaatacag tcaccgtaca agtcgcagag agcccccgtt tccggcaggc
     7681 aaaacagagc agcgaaaaaa gcagcggcgt gagcaggagt aaagcgaaca tcacagaata
     7741 actcatctca ttatcccttc agcgcggtca agttgttcac gtccagcgtg ccgtgggtac
     7801 gccatatttt tcttgccagt aacaccatca caatgacggc gaagatggcg tcggtagcga
     7861 tacctatttc caccagttcc ggtgctcgcc aggcaagaag cgccagcacc agatgggagc
     7921 cgttttccat caggcagtaa ccaaaaattt gccgcaggat attgcgctgg ctgacaatgc
     7981 acagcaggcc aagcagaaaa tgacctaacg ctaccgccag cgccggtttc agcccggtag
     8041 ccatcggtag cttcacgggc tgaacgacaa atgcgcaaag caggacaatt aacgccgcga
     8101 gcagtgccat cattgccgga ccgaataacg ctttttccgg gatgttctgg ggaatatttc
     8161 gtgcagcgta agtcatgatt aacggtacca gcagcacttt ggtgataaag gcgctggcgg
     8221 accagatcag cagttgctct gcggcgaaca ggcacgagag agtggcgaaa atagacacca
     8281 gcaccagcga ctggcaggcg taaaatccgc atgacaggcg atagcttttg acgctaatca
     8341 caaacagcga tgtcagcatc atcagtcccg ccagattatt tacgatcata gaaccggtca
     8401 ttcatcatct ccttactgca accaggtcgc ggcgattacg ctggaaatca gcgacatcac
     8461 aatcagtaca atcaacacca ggcgcattga ccctggcagc ggtgcggcat cggcgacggc
     8521 ctcgctcggt ttgcccggca cgacgcgacc aaaccagcga ataaaccagg cgaaactggc
     8581 gaccgattca atcatcagca gaatcatggc gggcagcagg atccagtact ccactgacaa
     8641 cgcaaaaccg acggcaaaca gcgggaattt actgaagaag ccgttgaacg gcggcacacc
     8701 ggtaatcgcc agcgctgcca cgcagaaacc cacgcttggc aacggcaggg tgtgcaatac
     8761 gccacgcaga cgcggcaaca agcgcgtgcc gcagctgtaa ctcagcgcac ctgctacaag
     8821 gaaaaacagg cttttagcga acgcgtggtt gacgatgtag gcgatgctac cctccagcgc
     8881 cagccgcgag ccgaagatgg agagcgacaa gccgaagaac atccagccaa gttgagtgat
     8941 ggtcgaccag gccagcaacc gcttcatatc ctgctgtggc aaatacatca gaaagccata
     9001 aagaatggtg accagcgcca tgaccatgcc aacgccgcca atcacatgcg ggatattgcc
     9061 gccgtcgata atggcgcggg caaaaatgta aacgcccact ttcaccatcg atgcggcgtg
     9121 gagataggca ctgatcggtg ttggcgcttc cattgcatcc ggtagccacg cttgcatcgg
     9181 tagctgggcc gatttccccc acgcggcaaa caggatgccg ccataaacca gataacgtgc
     9241 gtcgccgtgt aactcgctca tcgcgctaag cgcaaacgtt ccggtctgca aaaacagcgt
     9301 ggcggcggca agatacaacc ccaacgagcc gatatgagtg ataagtaacg cttttagtgc
     9361 tgaaagctgt gctttatcgc tctggtaata actgatcaac gcccaggagc agccgcccgt
     9421 aatttcaaaa aacaacaact gaccgagcag cgtcgaggag agtaccagtc ccgccatcgc
     9481 gccgataaac accagcaaaa atgcgtaata acgattcgtg ccgttatgcg ggtgttcgcg
     9541 atttttatcc gtcagataac ccgtggagta gatcgtgacc agcaaaccga gaaacaccac
     9601 cacaaacaga atcagcgtac tcatgcgatc aatgaccaag ccaaacaatg ccacatcccc
     9661 cacgttgacc aacgtcaacg tgacggcaac tttatcggcc tgataaaacg cggagatcag
     9721 cgacaacatg cacagcgtgg tcagtgcggc gaacaaaacc cccattcggc ggcccgacgt
     9781 tgtggcgaaa acgaaacgac cagtgcgccg ataaaaggca gcattaacgt cgtcagagca
     9841 agattttcca tattccgtca gtgctcctta cagaccggtt aaccagaaga cccatgcaag
     9901 cgcagcaagg ctgaagccaa gccaggtcac atggtgaatg agtaaaaaac gcccgcgtgc
     9961 cagcgtgttt tctgcgattg aggccagtac aaaaatcagc aaaaccttga gcagcgtaac
    10021 gacaagtgaa gtcagcaggc aggcgagaga aagttcttgc gcgcgcccaa agggcagaaa
    10081 cagggccaca aacagtgctg ccatcacgac ctgtttcagc cccagccccc atttcgctag
    10141 cgccagcccg gcaccggaat attcggtcag cgggccttcc tgtaattcct gttctgcttc
    10201 agcaacatca aagggaattt ttcccatctc aatgaagcag gcaaaaccac aggccagtaa
    10261 cgccagtacg gtggttagcg gcgagttcca gcccgttgcc agcgtattgc tgatcatctc
    10321 gatatgcgtg gaacctgcta tcagcgccaa taccagcagt gagagaatga gcatgggttc
    10381 gaccagaatg ccgagcgtca actcgcgact ggcaccgact cccgcaaacg ggcttccggt
    10441 atccagccct gaaagagcaa agaaaaaacg aaacagggca agaagataga taagggtgat
    10501 cagatcgccg ccgcccgcaa aaggggaaac ggtaataaat agtggtaggg ccatcgccag
    10561 caccagcatg ctgctgatta atacccacgg catcaggcgg aacatcagac ctgaagatgt
    10621 cggcgcatct tcctggcgtt taaacagttt gtggatatcg cgataatcct gccagatccc
    10681 cggcccgcgg cgggagtgca tacgcgcgcg tatctgccgg gaaatacccg taaaaagtgg
    10741 ggttagcatc agcagaatca cggcctgtgc taacgcaaaa atgaccagat atccgtcgca
    10801 aagagtttgt ctcatggtga tttccttaga cggcaatagc gattagcagc acaaccagtg
    10861 cggcgaccac atacaggcaa tagagacgaa agtcgccgct ttgcagatgc tgtatttctt
    10921 tggccagccg ttgggtggcg ctcacgatgg ggcggatcac ccgctcatcc cagaagggtt
    10981 ctgtgctctg agccctggcg gtgacgtgcg caagaccttt atttagcctc agcgtagggt
    11041 cgagctgttg tcgtagacga aatagcgcag aaaagaccac acgcagcggc tgcatcacgc
    11101 cattgcctga cggggccatc gcattttccc agccgtagcc gcatgcccag gcatctcctg
    11161 tgcgacgaaa cgctgcgcgg cgcgaacgac accacagcca gtaaaggcca ggcagcaaag
    11221 gcatcgccag tagtaacagc aacagtaatg aaggagtgac ccgtgtatga aacgtgccgg
    11281 gtacaagtgc tattccgctg gcgacagtga cgggaggggt attggtaaac gcatgggcaa
    11341 tgtgcattat cttcggtgcc agccaactgg cactaagcgc aattaatacg cagagtgccg
    11401 cgagcagtag catggcgacg atcatcgtat ttggcacttc ctgtgcctct tcagcgtgtg
    11461 tgctgcgcgg cgcaccacag aaagtaatac cgtacatttt gacgaagcac actactgcca
    11521 gcccaccagt gactgccagc attaccatag caataggacc cgcaagttgt agcgctacgg
    11581 cttccacacg acttagtgag aatagcgact gccaggtgta ccattcgctg ataaaaccat
    11641 tcagcggagg aatggctgat atcgccaggc aaccaatcag gcatgctgcg gctgtccacg
    11701 gcatccgttt cgccagtgcc cccatttttt ccatgtcgtg ggtatgcaaa cgcgaaataa
    11761 tcgcacccgc gccgagaaat agcagccctt tgaaaagcgc atggttgagc agatgaaaca
    11821 gtgcgccgag cagtccaacc acggtgagca gcgggtcgtg cagtgacaga ccgaccatcg
    11881 ccacaccgac cgccagcaaa ataatgccga cgttttcgac ggtactccag gccagcagcc
    11941 gtttgatatc ctgttccgcc agtgcataca gcacgcccag gagcgcggag attgcgccga
    12001 tcgccatcac cagaatgccc caccacagcg gcaaacccgt ttgcgccagc agatccatcg
    12061 ccactttcag gatgccgaaa ataccgattt tgaccattac gccagacatc aacgctgaag
    12121 cgtgcgatgg tgcggcaggg tgagcgcgcg gcaaccagct gtgcaacggc atcatcccgg
    12181 ctttcgcgcc aaaaccgaaa aaggccagca ggaacaccgc cgacgccagc cccggagaaa
    12241 gtgaaagcgt gcggaaactg gcaaaatcga ggctgccgct ttcgcgcccc atcagcaaga
    12301 aggcgatcat tatcagcacc gatccggcgt gggcgataaa aaagtagagc atgccagcgt
    12361 tgatcgacgt tttatcctgc ctggcaatga ccagaaacca ggaagacagc gacatcattt
    12421 caaacagcac gatgaaccaa aaagcgttgt ccatcaccag cagggcaacc atcgatgtga
    12481 tgaaaatatt cataaagaag cccatcgccg ccgcgccttt gccctcgtat tcgcgcatat
    12541 aagtcaatga atagagagaa caaaccacca ccagcaagga gataaccagc accataaatg
    12601 ccgagaggct atccattcgc agggttaaat cggcaaattc aaaaggcgtg gcaaaacggg
    12661 cgactaaagt tacgcctgca tgtaattgcg taattccgct gatgatgcca atcacgccgc
    12721 ctaccagcga tgtgatgccg gaaagcttaa tagccagtct gtccagaccg agtaaaaaca
    12781 gcgaagccag actggcaaac agatagagga tcagcgacca ggttaataat tgcagggcat
    12841 ccattagcgt tgctcctgag tgagggaaag gggatcctcc ccgccattgg caaaccagct
    12901 tgcggcaagg cgctgttttt ctttcatctg gcgttgcagg ctatcgtcgg tgatcagccg
    12961 taacgcctga ttcgggcaag cgcgaacgca ggccggacct tctggcaaga aatcacacag
    13021 gtcgcatttc accgcgacgg tctggacacc cggttcccag cgcagcaacg catgttgggg
    13081 cggcacgttt tcttcgccgt ctttgagtga gccctcagcc tgaaaaacat attgcgcagg
    13141 ggcattcacc ggacggcttc ctgaagcgct gattgcgcca aatgggcaga ccacggcgca
    13201 aagcttgcag ccaatacaga ggctttcgtt gagttggatc gcatcatccc tctgagagat
    13261 ggcattgacc gggcagacct gcaggcaagg ggcttcctca cagtgatgac acacgacagg
    13321 ggcagtgagt gttgacgtct tcgccagggc caggcgcggg tgttgctgta aaccttgcgt
    13381 tttatgcacg tccgaacagg cagcgagaca ggtattacat cctgtacacc acagtggttc
    13441 ggccaccaca aagcggttca tgtcggctcc ttgaaaatga tatgaaacat tgcatttcat
    13501 attgcataaa ggatgccatt ctgattaact caatatattc aataagttat gtattaactg
    13561 gattctgaac ggtacaaaaa tgatggtcat taacgttcag caaaaaatgg attcgacacc
    13621 cgtgtcgcag gggaaagcga gaatgtgatc ttgatcacga gaagtgaggt atgctggccg
    13681 caagcgcagc cagcacggaa tggagcaaag taatcaggcg tgttctttca gccagttcag
    13741 cacaacgtcg tggtgattgc tggttttgaa atcgtcaaag acatgttcga ttttgccatc
    13801 agcgtcaatc aggaagctga tgcgatgaat gccatcgtag gttttgccca tgaaggactt
    13861 ttcaccccag acgccgaatt gttcgcacac ctggtggtcc tcatcagaca ggagcgtaaa
    13921 gttaagcagc tctttttccg caaaacggga gagtttttcg ggtttatcgg tgctgatacc
    13981 cagcacatca acgcccgctt ttttcaactc atccatgtta tcgcgtaagc cgcaggcctg
    14041 tacggtacag ccgggggtca tggctttcgg gtagaaataa accagaacac gctgtccctg
    14101 gaagtcggtc aaattaactt gttctccgtc ttgatccggc aagctaaatt tcggtgcgat
    14161 atcaccggct ttcagtggat tcattactta actccatcct gttcatcatg ttgggaataa
    14221 ttgacgacgt taatactgcc ttgtgcattg agttctgtac atagggcttt gaacgcttgc
    14281 tcaatatttg ctgcgtccgc agatgcgggg ctgtgggcgg ttatctgaat atgcaactgc
    14341 gcagcccgtt cattttcagc aggttgcgtg cgcgacacca gctccgcaat gttcatatga
    14401 tgcgcgtcga aaagtgctgt gaagcgttca attaaatgcg gggagtctgc cacatcgacc
    14461 tgaacccaga cagatgctgg cattggcgga cgcggacgcg ccgtcgtgcg cttcatcacg
    14521 attaaaagat ccagctcggc acctttcaac ggtaacgttg attcaatcag agtaatggca
    14581 ttccatgaac cggaaagcag catgataaac gtgaactctt ctcccagcat cgccaggcga
    14641 ctgtcttcaa tattacagcc gcaactactg acatgacggg tgatggtgtt cacaattcca
    14701 gggcgatcgg cacccaatgc agtgatcacc agataatgtt gcgatgacag tgtcaaactg
    14761 gttattcctt taaggggtga gttgttctta aggaaagcat aaaaaaaaca tgcatacaac
    14821 aatcagaacg gttctgtctg cttgctttta atgccatacc aaacgtacca ttgagacact
    14881 tgtttgcaca gaggatggcc catgttcacg ggaagtattg tcgcgattgt tactccgatg
    14941 gatgaaaaag gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta tcatgtcgcc
    15001 agcggtactt cggcgatcgt ttctgttggc accactggcg agtccgctac cttaaatcat
    15061 gacgaacatg ctgatgtggt gatgatgacg ctggagctgg ctgatgggcg cattccggta
    15121 attgccggga ccggcgctaa cgctactgcg gaagccatta gcctgacgca gcgcttcaat
    15181 gacagtggta tcgtcggctg cctgacggta accccttact acaatcgtcc gtcgcaagaa
    15241 ggtttgtatc agcatttcaa agccatcgct gagcatactg acctgccgca aattctgtat
    15301 aatgtgccgt cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg tctggcgaaa
    15361 gtaaaaaata ttatcggaat caaagaggca acagggaact taacgcgtgt aaaccagatc
    15421 aaagagctgg tttcagatga ttttgttctg ctgagcggcg atgatgcgag cgcgctggac
    15481 ttcatgcaat taggcggtca tggggttatt tccgttacgg ctaacgtcgc agcgcgtgat
    15541 atggcccaga tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg cgttattaat
    15601 cagcgtctga tgccattaca caacaaacta tttgtcgaac ccaatccaat cccggtgaaa
    15661 tgggcatgta aggaactggg tcttgtggcg accgatacgc tgcgcctgcc aatgacacca
    15721 atcaccgaca gtggtcgtga gacggtcaga gcggcgctta agcatgccgg tttgctgtaa
    15781 agtttaggga gatttgatgg cttactctgt tcaaaagtcg cgcctggcaa aggttgcggg
    15841 tgtttcgctt gttttattac tcgctgcctg tagttctgac tcacgctata agcgtcaggt
    15901 cagtggtgat gaagcctacc tggaagcggc accgcttgcg gagcttcatg ccccggctgg
    15961 aatgattttg ccggtgacct ccggtgatta tgcaatcccg gtgaccaacg gtagtggtgc
    16021 tgtcggtaag gcgctggaca ttcgtccacc agcccagccg ctggcactgg tttctggcgc
    16081 gcgtacccag ttcacgggcg ataccgcttc attgctggtg gaaaatggtc gtggcaatac
    16141 tctgtggccg caggtggtta gcgtgctgca ggcgaaaaac tacaccatca cccaacgtga
    16201 tgatgctggt cagacactga ccaccgattg ggtacaatgg aaccgtctgg acgaagacga
    16261 gcagtatcgt ggtcgttatc aaatctctgt taagccgcag ggttatcagc aggcggttac
    16321 ggttaaactg ttgaacctgg aacaggcggg caaaccggtt gcagacgcgg cttccatgca
    16381 gcgttacagc acggagatga tgaacgttat ttccgccggt ctggataaat ctgccactga
    16441 cgccgcgaac gctgcgcaaa atcgtgcctc caccactatg gacgtacaaa gtgcagctga
    16501 tgacaccggt ttaccaatgc tggtcgtacg cgggccgttc aatgtggttt ggcaacgtct
    16561 gccagcggcg ctggaaaaag tgggcatgaa agtgaccgac agcacccgtt cgcagggcaa
    16621 catggccgta acttataagc cgctgtctga cagcgactgg caggaactgg gcgcgagcga
    16681 tccaggcctg gcatccggtg actataaact gcaggttggc gatttagata accgcagcag
    16741 cctacagttc atcgatccga aaggtcatac tctgactcag agtcagaacg acgcgctggt
    16801 agctgtcttc caggctgcgt ttagcaagta aaaatacagg gctggaatca tccggccctt
    16861 ttttctgata tgatacgcaa acgtgtgcgt cggcaggaaa acgcgatttt agcggtaatt
    16921 cgcacgaaat ttgtttgtcg gacgtagttc ggataaggcg ttcacgccgc atccgacaaa
    16981 acatccggca caccagacag caaaagattt taaaacgtta attcacaccc aggagtgata
    17041 aagatgcaaa agcaagctga gttgtatcgt ggtaaagcga aaaccgtata cagcacggaa
    17101 aacccggacc tgttggtgct cgaattccgc aatgatacgt cagcagggga tggcgcgcgc
    17161 attgagcagt ttgatcgcaa aggtatgttg aacaacaagt tcaactactt cattatgagc
    17221 aaactggctg aagcgggtat cccgactcaa atggagcgtc tgctctccga taccgaatgt
    17281 ctggtgaaaa agctggatat ggtgccggtt gagtgtgtcg tgcgtaaccg tgctgcgggc
    17341 tctctggtga aacgtcttgg aatcgaagaa ggtattgagc tgaacccgcc gctgttcgat
    17401 ctgttcctga aaaacgatgc catgcacgat ccgatggtca acgaatctta ctgcgaaacc
    17461 tttggctggg tgagcaaaga gaacctggcg cgtatgaaag agctgaccta caaagcgaac
    17521 gacgtgctga aaaaactgtt cgatgatgct ggtctgattc tggtcgactt caagctggag
    17581 tttggtctgt acaaaggtga agtggtactg ggcgatgagt tctccccgga cggcagccgc
    17641 ctgtgggaca aagaaacact ggagaaaatg gacaaagacc gtttccgcca gagcctcggt
    17701 ggcctgatcg aagcctatga agccgtcgcc cgccgcctgg gtgtacagct ggactgattt
    17761 ttctgttcat catcttgccg tgcagctggc acggcaagac aaccgctctc ggaatatatg
    17821 cattgaacgc tcattcctcc gccatttcat cccgctttcc tgtggtaatc ttcctctgaa
    17881 ccacctacga tcatttctat aatgaatata ttgttgaggt atctatgcgt tggcaagggc
    17941 gacgtgaaag tgacaatgtc gaagacaggc gtaacagctc cggcggtccg tcaatgggcg
    18001 gtcccggttt tcgcctgcca agcggtaaag gcgggctgat tttactgata gtcgtgctgg
    18061 ttgcaggcta ctatggtgtt gatttaaccg ggttgatgac cggacagccg gtttcccaac
    18121 agcaatcaac acggtcaatt agcccaaatg aagacgaagc cgcaaaattc acctcggtga
    18181 ttctggcaac cacggaagac acctggggac agcaattcga gaagatgggt aaaacctatc
    18241 agcaaccgaa gctggtcatg tatcgtggaa tgacgcgtac cggctgcggg gcgggccagt
    18301 ccattatggg accgttctat tgcccggcgg atggcacggt ttatatcgat ctctccttct
    18361 atgatgacat gaaagacaaa ctcggcgcgg atggcgattt tgcccagggg tacgttatcg
    18421 cccatgaagt cggtcatcat gtgcagaaat tgttaggcat cgagccgaaa gttcgtcaac
    18481 tgcaacaaaa cgcgacgcag gcggaagtaa accgcttatc tgtgcgtatg gaactccagg
    18541 ccgactgttt tgccggtgtc tgggggcata acatgcagca gcaaggcgat ctggaagagg
    18601 cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    18661 tagtaccaga cagtttcact catggtactt ctcagcaacg ctacagctgg tttaaacgtg
    18721 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg
    18781 ggcaaggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg
    18841 gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa
    18901 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa
    18961 ccactgttct ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    19021 cgacgcccgc cacggctttg atgccgctgc ctttgcggca cttagcggaa cgttgaaagc
    19081 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    19141 cgactcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    19201 tctcaaacgc gtacttacgg cggataacga cgctattctc tggcggcaaa accagccgtt
    19261 ctcgttggcg cattttaccc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    19321 agaacaacag caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac
    19381 ggctgcgcgt gggcgcggta agtccgcgct ggcagggcaa ctcatttctc gtattgcggg
    19441 cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    19501 cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc aagccgactg
    19561 gctggtggtc gatgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    19621 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggtaccg gacgtggttt
    19681 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    19741 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttaacga
    19801 tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg aacaaacgtt
    19861 atggcgaagc gatccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    19921 ccggacctcg ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca
    19981 ggcggctggc gaaaacgaga ttgccggggc gttgtggctg gtggatgagg gcggattatc
    20041 tcaagaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    20101 ccagtcgctg gcggcgcacg gcagcaatcc actggcggcg acattgcgcg gacggcgggg
    20161 cagccggatt gcagtccatc cggcgcgtca gcgcgaaggc gttgggcaac agctcattgc
    20221 cagcgctttg caatataggc ctggcctcga ctatctttcg gtgagttttg gttacaccgg
    20281 ggagttatgg cgtttctggc aacgctgcgg ttttgtgctg gtgcgaatgg gtaatcatcg
    20341 tgaagccagc agcggttgct atacggcgat ggcactgtta ccgatgagtg atgcgggtaa
    20401 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg
    20461 gaatggcgaa acgcttcctg ttgatccact aaacaatgcc gtcctttctg acgacgactg
    20521 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacgtcgt taggttgctt
    20581 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    20641 aaacaccagt gacgcgcagt tatgtaccac acttaaactt tcaggccgca agatgttact
    20701 ggttcgccag cgggaagagg ccgcgcaggc gctgttcgca cttaatgagg ttcgcactga
    20761 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    20821 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    20881 aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg ttactgcttt
    20941 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggcagctgg tttgcgccgc
    21001 tatttcctga tgcgctggtg gtgagtgtcg gaggcgcgga gccgagcggt aatccggcgg
    21061 ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    21121 ccatcatgcc gacgtttatt gagatggtgc gctactggca gaaacagagc ggagtagggg
    21181 ctaacgccac agcgctaatc ggtttctctc aaggcgcgat tatggcgctg gagagcatta
    21241 aagccgaacc gggccttgct tcacgcgtca ttgcttttaa cggacgctat gcgagcctgc
    21301 cggaaactgc gtctacagcc acgacaattc acctgattca tggcggtgaa gatccggtta
    21361 tcgatctggc gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    21421 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    21481 tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta agtggcggta
    21541 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    21601 acgcgtgagc gtcgcatcag gcatcgttca ccattgccgg atgcggcgtt aacgccttat
    21661 ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg gccagtcgtc
    21721 gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag
    21781 aaatttcttg tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa
    21841 aaagacaatc accagaatcc accaatattt agccagccag tccatgctta tttcctcttg
    21901 cagaaccact catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag
    21961 ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat tgaccggccc
    22021 gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg ttccgcctgt
    22081 ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac
    22141 cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccaatcca ccgtatagcg
    22201 cagttgatgt ttttcaagca gggcaagcac ctgcgctttg atcatctcat cagtcagttc
    22261 ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacgt tgttactgcc
    22321 cgttcccgcc tgaatattgg caatctgcat actggtcgcc gggaagaatt cattgccctg
    22381 atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta ctggattgtc
    22441 agccagatgt gggtaggcca catgcccctg cacgccatga atggtaaggt tgcaggttaa
    22501 cgatccgcga cgaccatttt tcaccacatc accaaccact tcgatactcg acggttcgcc
    22561 gaccagacag taatcgagac gctcattacg cgccattaac gcttcgacga cttttaccgt
    22621 accgttgtgg gcactggctt cttcatcaga ggtgatcaga aatgccagtc gtcccgtatg
    22681 gttgggatgt tgtgcgacaa aacgttctgc tgctaccacc atcgccgcca gcgagccttt
    22741 catatctgcc gcaccgcgcc cgaataacat gccatcacga atggtgggtt caaacggcgg
    22801 attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggccaa
    22861 tgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt ccatgcgttc
    22921 aacggtaaaa ccgatcgcct gcaaacgatc aatcaacaaa gcctggcatc ctgcgtcatc
    22981 aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat
    23041 agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca gcagcatagg
    23101 cttaccgggc gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgc
    23161 cgccgctgag gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt tacgccaggt
    23221 tgtaccacgg gtgttgagta acgcctccca acctaattcg ttgataaaac cgttcaataa
    23281 ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt tattggcttc
    23341 cagccaacgg cgagcctttt taatggtgtc acaatttttg ataccgt
//
LOCUS       DAZPJP010000050        22732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPJP010000050 DAZPJP010000000
VERSION     DAZPJP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.050"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHU9J_001794"
     CDS             complement(<1..454)
                     /gene="folK"
                     /locus_tag="ACHU9J_001794"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1905353.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTR"
     gene            complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHU9J_001795"
     CDS             complement(451..1848)
                     /gene="pcnB"
                     /locus_tag="ACHU9J_001795"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1905354.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHU9J_001796"
     CDS             complement(1908..2804)
                     /gene="gluQRS"
                     /locus_tag="ACHU9J_001796"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1905355.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHU9J_001797"
     CDS             complement(2871..3326)
                     /gene="dksA"
                     /locus_tag="ACHU9J_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1905356.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHU9J_001798"
     CDS             complement(3504..4208)
                     /gene="sfsA"
                     /locus_tag="ACHU9J_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1905357.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHU9J_001799"
     CDS             complement(4223..4753)
                     /gene="thpR"
                     /locus_tag="ACHU9J_001799"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1905358.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHU9J_001800"
     CDS             4827..7256
                     /gene="hrpB"
                     /locus_tag="ACHU9J_001800"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1905359.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7453..9987
                     /gene="mrcB"
                     /locus_tag="ACHU9J_001801"
     CDS             7453..9987
                     /gene="mrcB"
                     /locus_tag="ACHU9J_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1905360.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10207..12396
                     /gene="fhuA"
                     /locus_tag="ACHU9J_001802"
     CDS             10207..12396
                     /gene="fhuA"
                     /locus_tag="ACHU9J_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1905361.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12447..13244
                     /gene="fhuC"
                     /locus_tag="ACHU9J_001803"
     CDS             12447..13244
                     /gene="fhuC"
                     /locus_tag="ACHU9J_001803"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1905362.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13244..14134
                     /gene="fhuD"
                     /locus_tag="ACHU9J_001804"
     CDS             13244..14134
                     /gene="fhuD"
                     /locus_tag="ACHU9J_001804"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1905363.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14131..16113
                     /gene="fhuB"
                     /locus_tag="ACHU9J_001805"
     CDS             14131..16113
                     /gene="fhuB"
                     /locus_tag="ACHU9J_001805"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1905364.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16271..17551)
                     /gene="hemL"
                     /locus_tag="ACHU9J_001806"
     CDS             complement(16271..17551)
                     /gene="hemL"
                     /locus_tag="ACHU9J_001806"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1905365.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17717..17764
                     /gene="yadX"
                     /locus_tag="ACHU9J_001807"
     CDS             17717..17764
                     /gene="yadX"
                     /locus_tag="ACHU9J_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1905366.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17776..19197
                     /gene="clcA"
                     /locus_tag="ACHU9J_001808"
     CDS             17776..19197
                     /gene="clcA"
                     /locus_tag="ACHU9J_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1905367.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19221..19286
                     /gene="yadW"
                     /locus_tag="ACHU9J_001809"
     CDS             19221..19286
                     /gene="yadW"
                     /locus_tag="ACHU9J_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1905368.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19279..19623
                     /gene="erpA"
                     /locus_tag="ACHU9J_001810"
     CDS             19279..19623
                     /gene="erpA"
                     /locus_tag="ACHU9J_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1905369.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19670..20293)
                     /locus_tag="ACHU9J_001811"
     CDS             complement(19670..20293)
                     /locus_tag="ACHU9J_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1905370.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20331..21131)
                     /gene="btuF"
                     /locus_tag="ACHU9J_001812"
     CDS             complement(20331..21131)
                     /gene="btuF"
                     /locus_tag="ACHU9J_001812"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1905371.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21124..21822)
                     /gene="mtnN"
                     /locus_tag="ACHU9J_001813"
     CDS             complement(21124..21822)
                     /gene="mtnN"
                     /locus_tag="ACHU9J_001813"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1905372.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21906..>22732
                     /gene="dgt"
                     /locus_tag="ACHU9J_001814"
     CDS             21906..>22732
                     /gene="dgt"
                     /locus_tag="ACHU9J_001814"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1905373.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         5139 a         5793 c         6360 g         5440 t
ORIGIN      
        1 ctcttgtatg taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa
       61 tctcaaacag cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca
      121 ggcgttcggt atttatcact tcattaccaa acagcatgat atcgagatcc agcgtgcgtg
      181 gtccccagcg ttcagcttta cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt
      241 tgagtaactc ttccggggcg agcgaagttt caagcgccac ggcggcgttg aggtaatcgg
      301 gttgatcttg cggccccagc ggtggagtgc ggtaaaacga agaaacggca agaatgtggc
      361 tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca
      421 gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg
      481 tttgcgtgga cgacgagtac ggcgacgcgg tgacggctct tcatccaact cgttaagcat
      541 ccctttttga tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg
      601 cagttcagcg ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa
      661 cttaggatgc tccagcagtt tccatgcgcg tttaccctga cgtcgggaca tacgcaactg
      721 caactgccag atatcgcggg ttaacgtcgt caggcgtttc gggattgcca gtgaacggca
      781 ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct
      841 ttcctgggcg atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacaa
      901 gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg
      961 ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg
     1021 gaacagcggc tggaacagat gatattcaca caacagctta taggtttcgt aaccgtagcc
     1081 cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcagcag
     1141 ggtagcgagg cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc
     1201 ggcaaaacgt accgcgcgca gcatccgtac cggatcttca cggtagcgcg tttccgggtt
     1261 accaatcaga cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac
     1321 ggtaaaatcc gctacgctgt aatagaggct gttgatagtg aaatcgcggc gctgggcgtc
     1381 ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga
     1441 ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat
     1501 ctccgggcca aacattacat gagccagacg gaaacggcga cccaccaggc ggcagttacg
     1561 gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaaat ctttcggctt
     1621 tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt
     1681 gttgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg
     1741 ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc
     1801 ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg
     1861 tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca
     1921 tttgagaatg ttgaatttac aattgccgac tcgggcacgg cggtcagcgt ccagtttttg
     1981 actgctgact gaaggatttg ttcgacgctg aaatcctgcc agtgtgcttc tgcctgctga
     2041 cccagaaatt gaagtgccgc gattagtacc ggacgtggat cgcctttcgg caacgcaggc
     2101 gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg
     2161 taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccttac tgttggttca
     2221 atcagatcag ccccacgcac tatttctgta acgccctgga aatgatcatc aaccacaaca
     2281 gccaggttgt aggcgaacaa cccatcacgg cgatgaatga taaaatcttc ccgtgccagt
     2341 ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc
     2401 tggcggatac gcactgcggc attgtctggt ccatgatgca acacccggca atgaccgtcg
     2461 taaataccgc caatgctttg aatacgcgca cgcgtacagg tgcagtaata acttagtcct
     2521 tgttcatgta accaggcgag tgcttcacga taggcgtcgt gacgttgcga ttgccagaga
     2581 acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca
     2641 ccgggaactt cacgaggcgg gtcgatatct tctatgcgta ccagccagcg accttgccgg
     2701 gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag agccaaaatg aagctcgccg
     2761 gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa
     2821 taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc
     2881 atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg
     2941 cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct
     3001 tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg
     3061 cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg
     3121 aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga
     3181 ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct
     3241 tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat
     3301 gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca
     3361 tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc
     3421 taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt
     3481 aaattaacca gttacttact ttactacaat gtaaccgaca gtgatttttt aagagccatg
     3541 ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct
     3601 gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct
     3661 gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac gttcatcaac
     3721 tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc
     3781 tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac
     3841 ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag
     3901 cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa
     3961 agcgtattga cgcaaataaa tgcgccgctc tggctttgag ttagttccca ggtgtgtggg
     4021 tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccacg cgttgcacaa
     4081 ccggtcatcg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc
     4141 acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagag
     4201 aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc
     4261 gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcataagacc
     4321 agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat
     4381 gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca
     4441 tattcgccag ctggattaag ccgcgtggcg gctgacgcat ccctaaccac accacacgcg
     4501 aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc
     4561 cggctaaaag agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag
     4621 tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc
     4681 gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt
     4741 gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga
     4801 agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg
     4861 aattacttac cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg
     4921 ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta
     4981 tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg gcggagctgc
     5041 ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc
     5101 caaatacccg tctggaagta gttaccgaag gcgtgctgac gcggatgatc cagcgtgacc
     5161 cggagttgag cggtgttgga ttggtgatcc ttgatgaatt tcatgagcgc agcttgcagg
     5221 cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac
     5281 tgctgattat gtcggctacg ctggataacg accgcttgca gcaaatgctg ccagaagcgc
     5341 ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccattattta ccgctgcccg
     5401 cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc cgaaatgctg cgtcaggaaa
     5461 gcggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac
     5521 tggcttcgcg catcggcagt gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga
     5581 acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga
     5641 ccaatattgc tgaaaccagt ttaaccattg aaggtattcg tctggtggtg gattgtgccc
     5701 aggagcgtgt ggcgcgtttt gagccgcgca cggggcttac gcgactgatt actcaacgcg
     5761 ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatct
     5821 gcctgcattt aatcgccaaa gaacaagcag aacgcgctgc cgcgcaaagt gaaccggaga
     5881 tcttacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc
     5941 cggcgcagat gagctggctg gatcaaccgc cagtagtgaa tctaatggcc gcgaaacgtc
     6001 tgttacaaat gctgggggcg ctggacggtg aacggcttag tgcgcaaggg caaaaaatgg
     6061 cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg
     6121 aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccactg atgggcaata
     6181 gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac
     6241 tgttaaaacg cttaaacgta cgcggcggtg aggcagacag ttcgcttatc gcgccgctac
     6301 ttgccggggc gtttgccgat cgcattgctc atcgccgtgg gcaagatgga cgctatcaac
     6361 tggcaaacgg catgggagcg atgctcgatg ccgacgacgc gctaagccgc cacgaatggt
     6421 tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc
     6481 taccggtcga tattgatgaa ttagtacaac gctgtccgca gctggtgcag caatctgaca
     6541 ccgtggaatg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggcta caaatcggtc
     6601 agttgacggt gaaagtgcag ccgctggcga aaccgtcaga agacgagttg catcaggcga
     6661 tgcttaatgg catccgtgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac
     6721 agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg
     6781 ttgatgatga aagtttattg gcagcgctgg aaacgtggct gctgccacat atgacaggcg
     6841 tacattcact acgcggcctg aaatcactgg acatttatca ggcactacgc ggattacttg
     6901 attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg
     6961 gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcactggcg gtgagaatgc
     7021 aggagatgtt tggtgaggcc actaatccga ctatcgccca ggggggcgtg ccgctggtgc
     7081 tggagttgct ttcacccgcc caaaggccac tacaaatcac acgagatttg agcgccttct
     7141 ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacacgtct
     7201 ggccggacga cccggcaaac acggcaccga cgcgacggac gaaaaagtat tcgtaaggtt
     7261 ggttttctcc ctctccctgt gggagagggc cggggtgagg gcatcagcgt gcgcatttcc
     7321 ccctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacaa attttgagag
     7381 atatcttctt ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg
     7441 cggagaaaaa gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg
     7501 cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat
     7561 tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg
     7621 cgtaagcctc gtggcaaacg cggctggcta tggctgctgt taaaactggc tatcgttttt
     7681 gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat
     7741 ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa tggtcaacct tgaaccagat
     7801 atgaccatca gcaagaacga aatggtgaag ctgctggagg caacacagta tcgtcaggtg
     7861 tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgatccgc
     7921 cgtccgtttg atttcccgga cagcaaagaa gggcaggtgc gtgcgcgtct gacctttgat
     7981 ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt
     8041 cttgatccgc gcctgatcac catgatctct tcgccaaacg gtgagcagcg tctgtttgtg
     8101 ccgcgcagtg gtttcccgga cttgctggtg gatactttgc tggcgacaga agaccgtcat
     8161 ttctacgagc atgatgggat cagtctctac tccatcggac gtgcggtgct ggcaaacctg
     8221 accgccggac gcacggtgca gggagcgagt acgctgacgc aacagctggt gaaaaacctg
     8281 ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc
     8341 atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc
     8401 ggtcagagcg gcgacaacga aatccgcggc ttcccgcttg cgagcttgta ttactttggt
     8461 cgcccggtag aagagctcag cctcgaccag caggcgctgt tagtcggtat ggtgaaaggg
     8521 gcgtccatct acaacccgtg gcgtaacccg aaactggcgc tggagcgacg taatctggtg
     8581 ctgcgtttgc tacaacaaca gcagattatc gatggtgaac tgtatgaaat gctcagcgcg
     8641 cgtcctctgg gcgtacagcc gcgcggtggg gtaatctctc cacagccagc ctttatgcaa
     8701 ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg
     8761 aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa
     8821 ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc aattgtggtc
     8881 gtcgaccgct ttagtggtga agttcgtgcg atggtgggag gttctgagcc gcagtttgcg
     8941 ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact
     9001 tatctgacgg ctttaagcca gccgaaaatc tatcgcctga acacgtggat tgccgatgcg
     9061 ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaacga tgaccgtcgt
     9121 tatagcgaaa gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg
     9181 acggtaaatc tggggatggc gctggggctg cctgcagtta cggagacctg gatcaaactg
     9241 ggcgtgccga aagatcagct gcatccggtt ccggcgatgc tgctgggggc gttgaactta
     9301 acgccaatcg aagtggcgca ggcattccag accatcgcca gcggcggtaa ccgtgcaccg
     9361 ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg tgctgtatca gagcttcccg
     9421 caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag
     9481 gtggtacaac gtggtacggg tcgtcaactt ggggcgaaat acccgaacct gcatctggca
     9541 gggaaaacag ggactaccaa caataacgta gatacctggt ttgcgggcat tgacggcagc
     9601 acggtgacca tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggtgcc
     9661 agcggggcaa tgtctattta tcagcgttat ctggctaacc agacgccaac gccgctgaat
     9721 cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc
     9781 agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag
     9841 agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa
     9901 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9961 atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat ggcgaattgc
    10021 catccggtaa aataacatcc cacctaaagt attaaccctt tcttttcatc tggttgttta
    10081 ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10141 ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta catcagagat
    10201 ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10261 gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10321 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct
    10381 gcaactattg cggcgaagca ctctgctact gcgactaaaa cggatacacc aattgaaaaa
    10441 acgccgcagt ctatttcggt tgtgacaaat gaagagatgc agatgcgtca atttcagtct
    10501 gtaaaagaag cattaggtta tacacctggc gttacggtta gcagtcgtgg tgcttctaat
    10561 acatatgatt ttgttatcat tcgtggtttc tcatctgttg gtctgaatca aaataattac
    10621 cttgatggac taaaacttca gggcaacttt tataacgatg ctgtgattga tccttacatg
    10681 cttgagaggg ttgaactgat gcgtgggccg acgtctgttc tctatggaaa aagtaatcct
    10741 ggtgggatta tttctatggt gagtaagcgt ccgaccactg aacccctgaa agaaattcag
    10801 tttaaaatgg ggacggataa tctgtttcag accggattcg atttcagtga tgcactggat
    10861 gataacggtg agttctctta ccgtttgacg ggcctcgctc gctcaacaaa cgaacagcag
    10921 aaaaactctg aatctcagcg ttataccatt gctccatcat tctcatggcg tccagacgac
    10981 aaaaccaatt ttactttcct gtcctatttc cagaatgaac cagaaacggg ttattacggt
    11041 tggttgccga aagaggggac cgttgagcca ttgcctaatg gtaagcgtct accgactgac
    11101 ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc agaaaatggt gggatatagt
    11161 tttgaacatg gttttaatga caccttcacc gtgcgtcaga atctgcgttt cagtgaaatg
    11221 aaaacctcac agaaaagtgt ttatggcaca gggattgctg gtgatggtca taccctaaac
    11281 cgcgggacag tggtggataa tgagcgtcta caaaacttta gcgttgatac ccaacttgaa
    11341 agtaagtttg ctacaggtga cgtcgagcat actttgctga caggggtaga cttcatgcgg
    11401 atgcgtaatg atatcaatgc cagctttgga tccgcaccat ccatcgatct ttataacaaa
    11461 tatcaccctg aatactttgc atttggtagc gcagagccat accagatgaa tgaaagcaaa
    11521 caaacaggta tttatgttca ggatcaggcg gaatggaata aatgggtatt cactctgggg
    11581 ggacgctacg attggtctaa gcaagcgacc actgttcgtg aaaactctta tacgccgact
    11641 gaaggttata ttgagcgcaa tgatcatcag ttcacctggc gcggtggtgt aaattactta
    11701 ttcgataatg gtatttcacc ttactttagc tatagccagt cctttgaacc gagtgctttc
    11761 gatctgtgga gcaacccgcg catttcctat aagccatcga aaggtgaaca gtatgaagct
    11821 ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta cgggcgcagt ctatcaattg
    11881 acgaaaacaa ataacctgac agcagaccca acaaacccat tagcgcaagt cccagcaggt
    11941 gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg cgttaaatgc caatattaac
    12001 ttgacggctt cttataccta caccgatgcg gaatacacca aagacaccaa tctcaaaggt
    12061 aaaactccag aacaagtacc ggagcatatg gcatctctct ggggggatta taccttcaat
    12121 gaagggccgc tttctggttt aacattggga acaggtggtc gttttattgg ttccagctat
    12181 ggtgatccgg caaacacttt taaagtgggt agcgcagctg taatggatgc tgttgtaaaa
    12241 tatgatctgg cacgctttgg tatggcggga tccagccttg ctgtgaacgt caacaatttg
    12301 ctcgatcgtg agtatgttgc cagttgcttc cagacttatg gctgcttctg gggcgcagaa
    12361 cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttgggg cacggatttc
    12421 cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc gataccactt
    12481 ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt
    12541 taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct ggtaaatcca
    12601 ctctgctcaa aatgcttggt cgtcatcagc ctccgtcaga aggggagatt cttcttgatg
    12661 cccagccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct tatttgccgc
    12721 agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc
    12781 cgtggcatgg cgcgctgggg cgctttggtg cggcagatcg cgaaaaggtc gaggaagcta
    12841 tctcgctggt tggcttaaaa ccgctggcgc atcggctggt cgatagtctc tctggcggcg
    12901 aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt ctgttgctcg
    12961 acgaaccgac ttcggcgctg gatatcgccc accaggttga tgtgctggcg ctggtgcacc
    13021 gtttaagtca ggaacgtggc ctgacggtca ttgccgtgtt gcacgatatc aatatggcgg
    13081 cacgctactg tgattatctg gtcgccctgc gcagtggtga aatgattgct cagggaacgc
    13141 ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta tggcatcccg atgggtattt
    13201 tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg gcttacctct
    13261 tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat ggcagatgaa
    13321 taccgcccac gcggcggcta ttgaccccaa tcgtattgtg gcgctggagt ggttgccggt
    13381 ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggatacca tcaactatcg
    13441 cctgtgggtc agcgaaccac cattgccgga ctcagtgatt gacgtcggtt tgcgtacaga
    13501 acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt cggcaggata
    13561 tggcccttca tcagaaatgc tggcacgtat tgcgccgggt cgcggattta acttcagtga
    13621 cggcaaacag ccgttggcga tggcgcgtaa atcgctgacg gaaatggcag atttacttaa
    13681 cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa gactttatcc gcagcatgaa
    13741 accccgcttt gtgaagcgtg gcgcacgccc gttactgctg acgacgctta tcgatccgcg
    13801 ccatatgctg gtcttcggtc caaacagctt gttccaggaa attctcgatg agtacggcat
    13861 cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca gtatcgatcg
    13921 tctggcagcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca gcaaagacat
    13981 ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg ccggacgctt
    14041 tcagcgcgta cctgcagtct ggttttatgg tgcgacactc tcggcaatgc actttgtgcg
    14101 cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct ttttccggcg
    14161 ttattgctgg cgctgttagt gattgtcgct acggcgctca cctggatgaa cttctcgcag
    14221 gcgctgccgc gtagccagtg ggcgcaggct gcctggtcgc cagacatcga tgtcatcgag
    14281 cagatgattt ttcactacag cttgttgccg cgtctggcga tttcgctgct ggtgggcgcg
    14341 ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaacccgct ggcggagccg
    14401 acgacgcttg gcgttgctac aggcgcgcaa ctgggaatta ccgtcaccac actttgggcg
    14461 atcccaggtg ctatggcgag ccagtttgcc gcactggtgg gcgcttgtgt cgttggctta
    14521 atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg ttacgcttat tctcgcgggg
    14581 ctggtggtga gcctttattg cggggcaatt aatcagctac tggttatctt ccatcacgac
    14641 cagctgcaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga ctggggcggc
    14701 gttgagcgtt tatggccgca gttgctgggc ggcgtgatgt tgacgttatt gctacttcgc
    14761 ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct ggccttgtcg
    14821 ctcgcgcgtc tggcggcgct gtcgctggcg atagtcatca gtgcgctgct ggtgaatgcg
    14881 gtggggatta tcggctttat cggtttgttc gcgccgctgc tggcgaaaat gctgggggcg
    14941 cggcgtttgt tgccacggtt gatgctggcg tcgctgattg gggcgctgat cctctggctt
    15001 tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtgtccac cggttcggtc
    15061 actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg cagcattagc
    15121 gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt gctggcgttt
    15181 gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc gtttggtcgt
    15241 gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg aggatttgat gccctggcgc
    15301 tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc tggcggtggc gggctgtatt
    15361 attcagcggc tgaccggaaa cccgatggca agcccggaag tgctggggat tagctctggc
    15421 gcggcgtttg gcgtggtgtt aatgctgttt ctggtgccgg gtaatgcctt tggctggctg
    15481 ttacccgcgg gcagtatcgg ggcggcggtg acgctgttga tcattatgat cgccgccggc
    15541 cgcggtggat tttccccaca ccgtatgtta ctggcgggga tggcgttaag taccgcattc
    15601 accatgcttt tgatgatgtt gcaggcgagc ggtgacccgc gaatggcgca agtgctgacc
    15661 tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgtac cggaattgtg
    15721 atggtgattt tgctggcgat taccccgctg taccgccgct ggctgaccat tttaccgctg
    15781 ggtggtgata ccgcacgagc cgtaggaatg gcgctgacgc cgacgcgaat tgcgctgctg
    15841 ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta ttggaccgtt gagttttgtt
    15901 ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat gccacacatc
    15961 gtaatttcgg cgctggtggg tggtttactg ctggtattcg ctgactggtg tgggcggatg
    16021 gtgctgtttc cattccagat cccggcgggg ctgctgtcga cctttatcgg cgcgccatat
    16081 tttatctatt tgctgagaaa gcagagccgt taatttttcc atataacggc cctgtacgcc
    16141 tggacggata aagcgtttac gcgacaacgg atgcccgatg cgacgctggc gcgtcttatc
    16201 gggcctacaa acggccccga atcgtaggtc ggataaggcg ttcacgccgc atccgacaaa
    16261 ccatgctgga tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat
    16321 cttccatgct gtgcgccacg gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa
    16381 caccttcgtc cagcatcata tggaagaaac gcttaaagcg ttccacgtca caggccatca
    16441 catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac atgccgccaa
    16501 cgtggttaac gaccagcgga attccggctt cttctgccgc ttccagcaga ccttctgcca
    16561 gacgtgttgt cagctcatcc aacgtttcgt gaacgcccgg ttgcgcgact tcattcagac
    16621 aggcgaaacc ggctgccatc gcaattgggt taccggaaag cgtacccgcc tgatagaccg
    16681 gacccgtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca
    16741 ttccaccgcc gatgattttg cccaggcagg tgagatccgg ttccacaccg taataatcct
    16801 gtgcgccagc cagtgccacg cggaagccgg tcattacttc atcgatgatc agcaatgcgc
    16861 caaattcgtc gcacagcgca cgcagacctg gcaggaagtc tggcagcggt ggaacacagt
    16921 tcatattgcc tgccaccggc tcgatgataa tacaggcaat ctcttgcggg tattgctcaa
    16981 acgcggcgcg tacagaagcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat
    17041 ctgccggaac gcccggcgag tttggctggc ctaacgtgag tgcgccagaa ccggctttca
    17101 ccagcaggca gtcagcgtga ccgtggtaac acccttcaaa tttaatgatt ttgtcgcgac
    17161 cggtaaaacc acgggccaga cggatggcgc tcatcgtcgc ctcggtgccg gagttcacca
    17221 tgcgcaccat atccatggtc gggaccaatt cggtcaccag ttgcgccatt ttcacttcca
    17281 tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga
    17341 ttgccggatg gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg
    17401 ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc
    17461 cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg
    17521 ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga
    17581 atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt
    17641 gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc
    17701 tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca
    17761 ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc
    17821 ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt
    17881 ttgtttatgg cggcagtcgt cggcacgctt gttgggctgg cagcggttgc ttttgacaaa
    17941 ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat
    18001 ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt tggctacttt
    18061 ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg
    18121 ctggaagatc aacgtcccgt tcgctggtgg cgtgtattgc cggtgaagtt ctttggcggg
    18181 ctggggacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt gcagatcggc
    18241 ggtaacattg gccgtatggt gcttgatatt ttccgcctga aaggtgacga agctcgccat
    18301 acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg
    18361 ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgata
    18421 aaagcggtat ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa
    18481 gttgcgttga ttgacgtcgg taaactttct gatgcgccgc ttaatacgct gtggctttat
    18541 ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg
    18601 atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggc
    18661 ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac gtcgggcggc
    18721 ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt
    18781 atcttcgtcg ctcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt
    18841 atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc
    18901 gttgagctgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc cggaatgggg
    18961 gcattactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg
    19021 accgataact accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta
    19081 gcgcaattta ccggcgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa
    19141 caggaagctg agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg
    19201 aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga
    19261 tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca
    19321 gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc
    19381 gtgtatatca caggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag
    19441 gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcttggtggt tgatccgatg
    19501 agcctgcaat atctggtcgg cggttccgtt gactataccg aaggactgga aggttctcgt
    19561 ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc
    19621 taatctgttg ttcatgtgcc ggataaacta tccggcgcat cttctctctt caacgcccat
    19681 tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca
    19741 atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat
    19801 aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta
    19861 aaatcatggg gatctcgcgg gccagtacat cacgaatgat cccgccgcca acgccagtaa
    19921 tgacgaccat acaaaccgcg attaacggac cgacttccgc attaaaggct ttattcacgc
    19981 caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cattttggta
    20041 agcgtcttgg ctggcgcacc agcacgatgg tcagcatgct ggttaccatt gcaacgacca
    20101 gatcggtggg atctttcacc caaaataccg ggccgtgatc cagcgccatg tcgcgaattg
    20161 tcccgccgcc tacagcggta accacgccca gtaccagaac accaaaaggg tccatacgca
    20221 attttccggc taacaaaacg ccggagatgg caaataccgc tgtgccgact atatccagcc
    20281 aatagacgag cattgttcaa tccccactga gcacctaaaa aggtcaggcg ctaatctacc
    20341 tgtgaaagcg cattacagag ctgttgtgca gcgaggataa tacgtgggct tgcacgttca
    20401 aaccagtcac tcgtgagagg aataacggga attttgagct gttcacccca gtattgtttg
    20461 attttaggaa tttggtccgg tccgcctgta atgacaatcg cctgtggcga gcgtgctaac
    20521 acctgttcgc ggctaacttg cggccaggga acccggctgt ctttaaagat gttttctccg
    20581 ccacaaactt cgagtacctg gttttgaatc gactcttttc cactggtaaa tggcggatta
    20641 atgccgaatt gcagaaaaac acgtttttta ggtttatcag catattgcgc tttcaattgc
    20701 gcgtactgat ccagcaggga ttgcgcggct tgttcggcct tatccggttg cggactccag
    20761 ggggccaatt gacgtaacgc attggcaatt tgttcaatgc ttgtcgcatc gacccacatc
    20821 acttttattc ccagcgaagc cagctggtca acctgccgct cggcattacc tccacgccag
    20881 gcaatcacca gatcgggttt cagcgcgaca atgcgttcca gattcatccc ctgccaggtg
    20941 gaaacctgct caatcttttg cgcttgtgga ggatagtcgg aatagctgct gaccccaacc
    21001 ggcgtgatcc cggcggcaaa ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc
    21061 ggcgcggcgt tgagccacag tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt
    21121 gacttagcca tgtgccagtt tctgcaccag tgactcaacc atcaggctgg actgtttagc
    21181 ggcaacagcc aggaactcat cgaagctaag atgagactgt tgatcggcca cgtcggagat
    21241 ggcgcgtacg acaacaaacg ggacgttgaa attgtggcag acatgggcga ttgccgtcgc
    21301 ttccatctct acagcaatgg cctgtgggaa gttgtggcgg attttcgcca gaccaacaga
    21361 accgttgatg aaagcgtcgc cgctaacaat caggccacgt acagcgttaa gattcagttc
    21421 ggcaatgcag gcctcagcgg cagcgatcag tttatcgtca gctttaaagc ctgccggaca
    21481 gcctggtaac tgaccgtatt cataaccaaa tgccgtgaca tccgcgtcgt gataacgcgc
    21541 ttcgtccgag acaacgatat cgcccacttt cagcgttggt gccaggccac cggcagaacc
    21601 ggtgttaata atcacatctg gcttgcagtg ttccaacagc agagtggcac ccagcgccgc
    21661 agcgacttta ccgatgcccg atttcagaag cgcaacctcg gttccattca gttggccggt
    21721 atagatttcg caaccgccga gactgatagt ttgacggttt tcgattttgt cacgcagcag
    21781 cgtaacttct tcttccattg caccaatgat gccgattttc atagatttac tcgcgataag
    21841 cccgatttga aagcatagtt taccatgcgc ttacggggaa gcgtatttct cacgcgggag
    21901 aggacatggc acagattgat ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac
    21961 cgcagggcgt taaaaccgaa catgagatcc tgcggatctt cgagagcgat cgcgggcgta
    22021 tcatcaactc tccggcaatt cgtcgtctgc aacaaaagac ccaggttttt ccactggagc
    22081 gcaatgccgc cgtgcgcacg cgtcttaccc actcgatgga agtccagcag gtgggacgct
    22141 acatcgccaa agaaatttta agccgtctga aagagcttaa attgctggaa gcatacggcc
    22201 tggatgaact gaccggcccc tttgaaagca ttgttgagat gtcatgcctg atgcacgata
    22261 tcggcaatcc gccgtttggt cattttggcg aagcagcgat aaatgactgg tttcgccaac
    22321 gtttgcaccc ggaagatgcc gaaagccagc ctctgactga cgatcgctgc agcgtggcgg
    22381 cactacgttt acgggacggg gaagaacagc ttaacgagct gcggcgcaag attcgtcagg
    22441 acttatgtca ttttgagggg aatgcacaag gcattcgtct ggtgcataca ttgatgcgga
    22501 tgaatctcac ttgggcacag gttggcggta ttttaaaata tacccgtccg gcgtggtggc
    22561 gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg
    22621 aagaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc
    22681 cattaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca ga
//
LOCUS       DAZPJP010000051        22594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPJP010000051 DAZPJP010000000
VERSION     DAZPJP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.051"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHU9J_001815"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHU9J_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1905374.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHU9J_001816"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHU9J_001816"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1905375.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHU9J_001817"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHU9J_001817"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1905376.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHU9J_001818"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHU9J_001818"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1905377.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHU9J_001819"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHU9J_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1905378.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHU9J_001820"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHU9J_001820"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1905379.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHU9J_001821"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHU9J_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1905380.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHU9J_001822"
     CDS             complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHU9J_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1905381.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="ACHU9J_001823"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="ACHU9J_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1905382.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="ACHU9J_001824"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="ACHU9J_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1905383.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="ACHU9J_001825"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="ACHU9J_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1905384.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="ACHU9J_001826"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="ACHU9J_001826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1905385.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="ACHU9J_001827"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="ACHU9J_001827"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1905386.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="ACHU9J_001828"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="ACHU9J_001828"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1905387.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="ACHU9J_001829"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="ACHU9J_001829"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1905388.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHU9J_001830"
     CDS             14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHU9J_001830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1905389.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHU9J_001831"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHU9J_001831"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1905390.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHU9J_001832"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHU9J_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1905391.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHU9J_001833"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHU9J_001833"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1905392.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHU9J_001834"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHU9J_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1905393.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHU9J_001835"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHU9J_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1905394.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHU9J_001836"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHU9J_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1905395.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHU9J_001837"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHU9J_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1905396.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHU9J_001838"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHU9J_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1905397.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22594
                     /locus_tag="ACHU9J_001839"
     CDS             22225..>22594
                     /locus_tag="ACHU9J_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1905398.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5055 a         5671 c         6429 g         5439 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat ggcg
//
LOCUS       DAZPJP010000052        22434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPJP010000052 DAZPJP010000000
VERSION     DAZPJP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.052"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHU9J_001840"
     CDS             complement(<1..223)
                     /locus_tag="ACHU9J_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1905399.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHU9J_001841"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHU9J_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1905400.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHU9J_001842"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHU9J_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1905401.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHU9J_001843"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHU9J_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHU9J_001844"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHU9J_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1905402.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHU9J_001845"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHU9J_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1905403.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHU9J_001846"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHU9J_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1905404.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHU9J_001847"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHU9J_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1905405.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHU9J_001848"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHU9J_001848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1905406.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHU9J_001849"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHU9J_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1905407.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHU9J_001850"
     CDS             7306..8433
                     /locus_tag="ACHU9J_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1905408.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHU9J_001851"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHU9J_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1905409.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHU9J_001852"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHU9J_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1905410.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHU9J_001853"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHU9J_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1905411.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHU9J_001854"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHU9J_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1905412.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHU9J_001855"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHU9J_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1905413.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHU9J_001856"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHU9J_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1905414.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHU9J_001857"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHU9J_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1905415.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHU9J_001858"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHU9J_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1905416.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHU9J_001859"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHU9J_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1905417.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHU9J_001860"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHU9J_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR1905418.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHU9J_001861"
     CDS             14427..15242
                     /locus_tag="ACHU9J_001861"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1905419.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHU9J_001862"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHU9J_001862"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR1905420.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHU9J_001863"
     CDS             complement(17104..17286)
                     /locus_tag="ACHU9J_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1905421.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHU9J_001864"
     CDS             complement(17365..18282)
                     /locus_tag="ACHU9J_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1905422.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHU9J_001865"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHU9J_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHU9J_001866"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHU9J_001866"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1905423.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHU9J_001867"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHU9J_001867"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1905424.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHU9J_001868"
     CDS             complement(21301..21957)
                     /locus_tag="ACHU9J_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1905425.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHU9J_001869"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHU9J_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1905426.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5501 a         5901 c         5685 g         5347 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttac
//
LOCUS       DAZPJP010000053        22343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPJP010000053 DAZPJP010000000
VERSION     DAZPJP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.053"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            97..1332
                     /gene="moeA"
                     /locus_tag="ACHU9J_001870"
     CDS             97..1332
                     /gene="moeA"
                     /locus_tag="ACHU9J_001870"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1905427.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            1332..2081
                     /gene="moeB"
                     /locus_tag="ACHU9J_001871"
     CDS             1332..2081
                     /gene="moeB"
                     /locus_tag="ACHU9J_001871"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1905428.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2260..2922)
                     /gene="fsa"
                     /locus_tag="ACHU9J_001872"
     CDS             complement(2260..2922)
                     /gene="fsa"
                     /locus_tag="ACHU9J_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1905429.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            3053..3952
                     /locus_tag="ACHU9J_001873"
     CDS             3053..3952
                     /locus_tag="ACHU9J_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1905430.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3958..6390
                     /locus_tag="ACHU9J_001874"
     CDS             3958..6390
                     /locus_tag="ACHU9J_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1905431.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6536..7351
                     /gene="ybiV"
                     /locus_tag="ACHU9J_001875"
     CDS             6536..7351
                     /gene="ybiV"
                     /locus_tag="ACHU9J_001875"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1905432.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7503..8768
                     /locus_tag="ACHU9J_001876"
     CDS             7503..8768
                     /locus_tag="ACHU9J_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1905433.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(8735..9895)
                     /locus_tag="ACHU9J_001877"
     CDS             complement(8735..9895)
                     /locus_tag="ACHU9J_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1905434.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNFSSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            10133..11434
                     /locus_tag="ACHU9J_001878"
     CDS             10133..11434
                     /locus_tag="ACHU9J_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905435.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            11449..13812
                     /locus_tag="ACHU9J_001879"
     CDS             11449..13812
                     /locus_tag="ACHU9J_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR1905436.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(13992..15587)
                     /locus_tag="ACHU9J_001880"
     CDS             complement(13992..15587)
                     /locus_tag="ACHU9J_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1905437.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            15806..16726
                     /gene="ldtB"
                     /locus_tag="ACHU9J_001881"
     CDS             15806..16726
                     /gene="ldtB"
                     /locus_tag="ACHU9J_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1905438.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16785..17903)
                     /locus_tag="ACHU9J_001882"
     CDS             complement(16785..17903)
                     /locus_tag="ACHU9J_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1905439.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(17900..18367)
                     /gene="mntR"
                     /locus_tag="ACHU9J_001883"
     CDS             complement(17900..18367)
                     /gene="mntR"
                     /locus_tag="ACHU9J_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1905440.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18553..18681
                     /gene="mntS"
                     /locus_tag="ACHU9J_001884"
     CDS             18553..18681
                     /gene="mntS"
                     /locus_tag="ACHU9J_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1905441.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18953..20536
                     /gene="opgE"
                     /locus_tag="ACHU9J_001885"
     CDS             18953..20536
                     /gene="opgE"
                     /locus_tag="ACHU9J_001885"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1905442.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20585..21100)
                     /gene="ompX"
                     /locus_tag="ACHU9J_001886"
     CDS             complement(20585..21100)
                     /gene="ompX"
                     /locus_tag="ACHU9J_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1905443.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(21153..21218)
                     /locus_tag="ACHU9J_001887"
     CDS             complement(21153..21218)
                     /locus_tag="ACHU9J_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905444.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            21453..22340
                     /gene="rhtA"
                     /locus_tag="ACHU9J_001888"
     CDS             21453..22340
                     /gene="rhtA"
                     /locus_tag="ACHU9J_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1905445.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         5343 a         6041 c         5725 g         5234 t
ORIGIN      
        1 ggcaggagcc aacgctacgc ccgaggtggc atacagtctg tggcgttttt ctgacataat
       61 aggcaaattc gattttgcct ccgcaggagt gttttcatgg aatttaccac cggattgatg
      121 tcgctcgaca ccgcgcttaa tgagatgctt tctcgcgtca ccccactgac cgcccaggaa
      181 acgctgccac tggtacagtg ttttggtcgt attctggcga gcgatgtcgt ttcgccgctt
      241 gatgttccgg ggtttgataa ctccgcaatg gacggctacg cggtgcgttt agccgatatt
      301 gcctccgggc aaccgctgcc cgttgccggt aaatcctttg ccggtcagcc gtatcatggt
      361 gaatggcctg cgggcacctg cattcgtatt atgaccggtg cgccggtgcc ggaaggctgc
      421 gaagcggtgg tgatgcagga gcagactgaa caaacggaca atggcgtgcg ttttactgct
      481 gaagtgcgta gcgggcaaaa tattcgccgt cgcggtgaag atatctctgc aggtgcggtt
      541 gttttcccgg cgggaactcg cctgactacc gcagagctgc cagtaattgc ttcactgggg
      601 attgccgaag ttccggtgat tcgtaaagtg cgtgtagcgc ttttttctac cggtgatgaa
      661 ctccagttgc ccggtcagcc gctgggcgac ggccaaatct acgacaccaa ccgtctcgcc
      721 gtacacctga tgttagaaca gctgggttgc gaggtaatca acttagggat tatccgcgac
      781 gatccccatg ccctgcgcgc cgcatttatt gaagccgaca gccaggcgga tgtagtgatc
      841 agttccggcg gtgtttcagt gggtgaagcg gattacacca aaacgattct tgaagagctg
      901 ggggagatcg ccttctggaa gctggcgatt aaaccaggta aaccgttcgc gttcggtaaa
      961 ctcagcaata gctggttctg cggcctgccg ggcaacccgg tttcagcgac gctgaccttc
     1021 tatcaactgg tacagccttt gctggcaaaa ctaagcggca acaccgccag cggcctgccc
     1081 gcgcgccagc gcgtacgcac agcgtcccgc ctgaaaaaaa cgccaggacg tcttgatttc
     1141 cagcgcggcg tgctgcaacg caacgccgac ggtgaacttg aagtgacgac caccggacat
     1201 cagggttcac atatatttag ctcctttagt ctcggcaact gctttatcgt gctggaacgc
     1261 gatcgcggca atgtggacgt gggcgaatgg gtggaagtag aaccgtttaa cgcgttgttc
     1321 ggaggcctgt aatggcggaa ctcagcgatc aggagatgct gcgctacaac cggcaaatca
     1381 ttctgcgcgg ttttgatttt gacggtcagg aggcgctaaa agattctcgt gtgctgatcg
     1441 tcggcctcgg tggactcggc tgcgcagctt cgctgtatct ggcaagcgcc ggtgtcggca
     1501 acctgacgct gctcgacttc gacacggttt cgctctcgaa tctgcaacgc cagacactgc
     1561 acagtgatgc cacggtcggg caaccgaagg tggaatccgc ccgtgacgcc ctgacgcgga
     1621 tcaacccaca tatcgcgatt acgccagtca atgcactgct agatgacgca gaacttgcag
     1681 cattgattgc tgaacacgat ctggtgctcg actgtacgga taacgttgcg gtacgtaatc
     1741 aactgaacgc aggctgtttt gccgcgaagg tgccgctggt ttccggcgcg gcaattcgta
     1801 tggaagggca aatcacggtc ttcacttatc aggacggcga accgtgctat cgctgcctta
     1861 gccgtttgtt tggcgagaac gcattaacct gcgtagaagc aggcgtgatg gcaccgttga
     1921 tcggcgtaat tggttcgttg caggcaatgg aagcgatcaa actgctggca ggttatggca
     1981 aaccgaccag cgggaaaatc gtcatgtacg atgcgatgac ctgtcagttt cgcgaaatga
     2041 aactgatgcg caatccgggg tgtgaggtgt gcgggcagta attgccacct ccaggcctga
     2101 taagacgcga caagcgtcgc atcaggcatt tgctccagac gccagatgcg gcgtgaacgc
     2161 cttatctggc ctacggttcg ggcacagatt tccaagcgcg gcaggcatcg gtgcactccg
     2221 ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa
     2281 cgctccctgc cagtcctgct caaacttcgc cacagcggct tccaccgccg gatagctaat
     2341 catctgttgt gccacatcca gtggcagagt aattgattca catcctgcca gtaagcagtc
     2401 cagcgcctga cgcggggttt tgaaactcgc tgccagcact ttcgcctgcg gcgcatgcat
     2461 tttcaataac tggtgtaagt cggtcacagt ctgaatgccg ctaccgccct gagcatcaat
     2521 acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagca acccttgtgc
     2581 tgcgccatat accgcggttc ccagcgttgg aatcccttcc gcttttaaca tcttaatagc
     2641 tgccagcccc tcggcggtca ccggaacttt caccacgata tccgcaataa tagaacgcaa
     2701 tttacgtgcg tcattcacca tcccttcagc agtggtagcc attacctggg caaacagacg
     2761 cccctgaccg cccatcgctt catgaagttg cggaagcaca acatccagcg gttttttacc
     2821 cgcggcgata atgcttgggt tagtggtcac acccgccagc ggaaaaatac gtgacagcgc
     2881 cttcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta
     2941 ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac gcacatcaat
     3001 ctaactttca ttcgaaagta ttttaatgtt tgaacgaaac agagaggcaa ctatgatttt
     3061 caatattcag cgctactcga cccatgatgg ccccggtatc cgcacggtcg tatttcttaa
     3121 aggctgttcg ctgggctgcc gctggtgtca gaacccggaa agccgcgccc gcacgcagga
     3181 tctgctgtat gacgcacgac tgtgtctgga aggctgcgag ctgtgcgcta aggccgcgcc
     3241 ggaagtgatt gagcgcgcgc tgaatggttt gcttattcat cgggaaaagt taaccccgga
     3301 gcatctgacg gcgttaaccg actgctgtcc gacacaggca ttaaccgtgt gtggtgaagt
     3361 gaaaagcgtt gaggagatca tgacgaccgt tctgcgcgat aaaccgtttt acgatcgcag
     3421 cggcggcggt ttaacgcttt cgggtggtga gccctttatg cagccggaaa tggcgatggc
     3481 gctactgcaa gccagccacg aggcaggcat tcatactgcg gtagaaacct gtctgcatgt
     3541 gccgtggaaa tatatcgccc cttctctgcc ctatatcgat ctgtttcttg ccgatttaaa
     3601 acacgttgcc gacgcgccgt ttaaacagtg gaccgacggt aacgccgcca gagtgctgga
     3661 taacctgaaa aaactcgccg cagcgggcaa aaaaatcatt atccgcgtgc cgctgattca
     3721 gggctttaat gccgacgaaa cctctgtaaa agctattacc gattttgccg ccgacgagct
     3781 gcacgttggc gaaattcatt tcctgcccta ccacacgcta ggcatcaaca aatatcactt
     3841 acttaatctg ccctatgacg ccccggaaaa accgcttgat gcgccagaac tgctcgactt
     3901 tgcccagcag tatgcctgcc agaaagggtt aaccgcgacc ttacgaggat aacaaccatg
     3961 accacactga aactggacac gctcagcgac cgcattaaag cgcacaaaaa tgcgctggtg
     4021 catattgtga aaccgccagt ctgtaccgag cgcgcgcagc actataccga gatgtatcaa
     4081 caacatctcg ataagccgat cccggtacgt cgcgcgctgg cactggcgca tcacctggcg
     4141 aatcgcacca tctggatcaa acacgatgag ttgatcattg gcaaccaggc aagcgaagtt
     4201 cgcgccgcgc cgatcttccc ggaatatact gtctcgtgga tcgaaaaaga gattgatgat
     4261 ctggcagatc gtcccggtgc tggctttgcg gtgagcgaag agaacaaacg cgttctgcat
     4321 gaagtgtgcc cgtggtggcg cggtcagacc gtacaggatc gctgctacgg catgtttacc
     4381 gatgagcaaa aaggtctgct ggcgaccgga atcattaaag cggaaggcaa tatgacctcc
     4441 ggcgatgcgc acctggcggt gaatttcccg ctgctgctgg aaaaaggtct tgatggtctg
     4501 cgcgagaaag tagcggaacg tcgctcgcgc atcaatctga cggtactgga agatttgcac
     4561 ggcgaacagt tcctgaaagc gattgatatc gtgctggtgg cagtcagcga acacattgaa
     4621 cgttttgcag ccctggcgcg tgaaatggcc gcgaccgaaa cccgcgaaag ccgtcgcgat
     4681 gaactgctgg cgatagcaga aaactgcgat cttatcgccc accagccgcc gcagactttc
     4741 tggcaggcgc tgcaactgtg ttacttcatc caattgatat tgcagatcga atctaacggt
     4801 cactcggtat cgtttggtcg tatggaccag tatctctacc cgtactatcg ccgcgacgtt
     4861 gaactcaacc agacactgga tcgcgaacac gccatcgaat tgctgcatag ctgctggctg
     4921 aaactgctgg aagtgaacaa gatccgctcc ggctcacact caaaagcctc tgcgggaagt
     4981 cctctgtatc agaacgtcac cattggcgga caaactctgg ttgatggtca accaatggac
     5041 gcggtgaatc cactctctta cgctatcctc gaatcctgcg gtcgcctgcg ttccactcag
     5101 cctaacctca gcgtgcgtta ccatgcagga atgagtaatg atttcctcga cgcctgcgta
     5161 caggtgatcc gttgcggctt cgggatgccg gcgttcaaca acgacgaaat cgtgatcccg
     5221 gaatttatta aactcggtat tgaaccgcag gacgcttacg actacgcagc gattggttgt
     5281 atcgaaaccg ccgtcggtgg caaatggggc tatcgctgta ccggcatgag ctttatcaac
     5341 ttcgcccgcg tgatgctggc ggcgctggaa ggcgggcgtg atgccaccag cggcaaagtg
     5401 ttcctgccac aagaaaaagc gttgtcggca ggcaacttta acaatttcga tgaagtaatg
     5461 gacgcgtggg atacgcaaat ccgttattac acgcgtaaat caatcgaaat cgaatatgtc
     5521 gtcgacacca tgctggaaga gaacgtgcac gatattctct gctcggcgct ggtggatgac
     5581 tgtattgagc gagcgaaaag tatcaagcaa ggcggcgcga aatatgactg ggtttctggc
     5641 ctgcaggttg gcattgccaa cctcggcaac agcctggcgg cagtgaagaa actggtattt
     5701 gaacagggcg cgattggtca gcaacagctg gctgccgcac tggcggatga cttcgatggc
     5761 ctgactcatg agcaactgcg tcagcggctg attaacggtg cgccgaagta cggcaacgac
     5821 gatgataccg tcgatacgct gctggctcgc gcttatcaga cctatatcga cgaactgaaa
     5881 cagtaccata acccgcgcta cggtcgtggt ccggttggcg gcaactatta cgcgggtacc
     5941 tcgtctatct ccgctaacgt accgtttggc gcgcaaacta tggcaacacc ggacgggcgt
     6001 aaagcccaca ccccgctggc agaaggcgca agcccggcct ccggtactga ccatcttggc
     6061 cctactgcgg tcattggctc agtgggtaaa ctgcctacgg cagcgattct cggcggcgtg
     6121 ttgctcaacc agaaactgaa tccggcaacg ctggagaacg aatctgacaa gcagaaactg
     6181 atgatcctgc tgcgtacctt ctttgaagtg cataaaggct ggcatattca gtacaacatc
     6241 gtttcccgcg aaacgctgct ggaggcgaaa aaacatccgg atcagtaccg cgatctggta
     6301 gtgcgtgtcg cgggctattc cgccttcttc accgcgctct ctccagacgc acaggacgat
     6361 atcatcgccc gtactgaaca tatgctgtaa tctcctcggc ccggcggcgt cgtcgccggg
     6421 ctaaatcatt cacatcatca atttcatcct tactttcatt cgaaatataa tttgtgctct
     6481 gcgtcacatt gttattacac tgttacggtt tcattttcga gccaggagca cacctatgag
     6541 cgtaaaagtt atcgtcacag acatggacgg tacttttctt aacgacgcca aaacgtacaa
     6601 ccaaccacgt tttatggcgc aatatcagga actgaaaaag cgcggcatta agttcgttgt
     6661 cgccagcggt aatcagtatt accagcttat ttcgttcttt cctgagctaa aggatgagat
     6721 ctcttttgtc gcggaaaacg gcgcactggt ttacgaacat ggcaagcaac tgttccacgg
     6781 cgaactgacc cgacatgaat cgcggattgt tattggcgag ttgctaaaag ataagcaact
     6841 caattttgtc gcctgcggtc tgcaaagtgc atatgtcagc gaaaatgccc ccgaagcatt
     6901 tgtcgcactg atggcaaaac actaccatcg cctgaaagct gtaaaagatt atcaggagat
     6961 tgacgacgta ctgttcaagt tttcgctcaa cctgccagat gaacaaatcc cgttagtgat
     7021 cgacaaactg cacatagcgc tcaatggcat tatgaaaccc gtcaccagtg gttttggctt
     7081 tatcgacctg attattcccg gtctacataa agcaaacggt atttcgcggt tactgaaacg
     7141 ctgggatctg tcaccgcaaa atgtggtagc gattggcgac agcggtaacg atgcggagat
     7201 gctgaaaatg gcgcgttatt cctttgcgat gggcaatgct gcggaaaaca ttaaacaaat
     7261 cgcccgttac gctaccgatg ataataatca tgaaggcgcg ctgaatgtga ttcaggcggt
     7321 gctggataac acatcccctt ttaacagctg acctctctac cggagccgtg cgctccggtt
     7381 ttcccttctt tttacatcct gtacatcaca tcaaatcatt aaacttgcat taacttattt
     7441 ataacttata ttgtcgcatg caaatcagaa tggtttcgaa tgaaagttaa tgagaggtgg
     7501 ttatggcttc tacttttacc agcgacacat tgcctgccga tcacaaagca gctatccgtc
     7561 agatgaagca cgcgctgcgg gcgcagcttg gcgacgtcca gcagatcttt aatcagctaa
     7621 gcgatgacat tgccacgcga gtggctgaaa tcaacgcact caaagcacag ggcgatgccg
     7681 tctggccggt gctgtcttat gccgatatca aagcaggtca tgttactgca gagcagcgcg
     7741 aacagattaa acgtcgcggt tgtgcggtga taaaaggcca tttcccccgc gaacaagcgc
     7801 taggctggga tcagtcgatg ctggactatc tggaccgcaa ccgctttgac gaggtctaca
     7861 aaggccccgg cgataatttc ttcgggacgc tcagcgcttc acgtcccgag atttacccca
     7921 tctactggtc gcaggcgcaa atgcaggccc gccagagtga agaaatggcg aatgcgcagt
     7981 cgtttctcaa tcgtctgtgg acatttgaaa gtgatggaaa gcaatggttt aacccggatg
     8041 tgagcgtcat ctaccctgac cgtatccgcc gccgtccgcc cggaacgacc tccaaaggtc
     8101 ttggagcgca taccgactcc ggggcgctgg aacgctggct gcttccagcg tatcagcgcg
     8161 ttttcgccaa cgtctttaat ggcaatctgg cgcaatatga tccctggcat gcggcacatc
     8221 gtacggaagt tgaagagtac acggtggaca acaccaccaa atgttccgtg tttcggacat
     8281 tccaaggctg gacagcgctc tctaatatgc tgcctggtca ggggctgctg cacgttgtgc
     8341 ccattcctga agccatggcg tacgtactgt tacgtccgct gcttgatgat gtgccggagg
     8401 atgaactgtg cggcgtagcg cccggaagag tgttgccggt atcagagcaa tggcatccac
     8461 tgttaattga ggcgttaacc agcattccaa aactcgaggc cggagactcc gtctggtggc
     8521 actgcgacgt catccattcc gttgcccccg ttgaaaatca acagggttgg ggcaacgtga
     8581 tgtacattcc tgcggcacca atgtgcgaga aaaatcttgc ctacgcgcac aaggtgaagg
     8641 ccgcactgga aaaaggcgca tcgccgggcg acttcccgcg cgaggactat gaaacaaact
     8701 gggaaggacg ctttacgctt gccgacctca acattcacgg taagcgagcg ttgggaatcg
     8761 acagctgagt gtcgtacagc ccttcccgtt ccacggcggt gttccctttt cctgggcgaa
     8821 cccgccgcac tgactcccgc accgccaatc gtccgtgcag cagcagcgag cccaccggag
     8881 catccgggcg aatcagccgc tgttgcaaca tttgcaccgc ttcattgcct agctcatcgc
     8941 gtggcacatg cacagccgtc aggggaacat cctcgattgc cgcaaggtta aagccgtcga
     9001 tactcatcac cgagacatcc tgtggtacgc gcagaccacg acgatgtagc gcatttaccg
     9061 ttcccgccgc cataaaatcc ccgccgacca gaaacgccgt cggcaacgtc ttgccattgg
     9121 cggcatccag ccacgctccg accttttctt ccgcttcttt agcgctaaaa ctgctgaaat
     9181 tcagcaaatg acggtcatgg tgaaaggcca gattatgctg acgccatgat tctttaatcc
     9241 ctgcaaaccg ctgctccata gtgtagcggc gcaggcacat cacattcatc acctcccggt
     9301 gacccatttc aaacagatag cgggcggcga acgcgccaat taaccgatga tccggcgcaa
     9361 tcgccgggag gcgcattcgt tcatcacggc agttgatcag cacacaaggc ttagcgagat
     9421 cggcggccag atcatggatg tgcggatcgt caatccccag caggatcgct gcctgcgtat
     9481 ctgcttcatt catccgcgag agaaagagcg cgggatcgct gtcaaactcc tccagcgcgc
     9541 agtaacgcag ccgcacttca tgcggcgata acgctttgct gatgctctgg atcacacggt
     9601 aataaaagat atcagatcgt tggtcaaaag cgcgttgcgg cgcgaaaata atcaggttgt
     9661 tcagcaacat acgacctgct gccatgccat ccatcacgcc caactcacgc gcgcaggcca
     9721 gcacctgttc gcgagcgcgt gcactggtat tcgctttgcc agccagcacc cgcgaaaccg
     9781 tactcggcga tagcccggta cgtgcggcga tatcggcgat tttcagcttc ttgtccattt
     9841 tgtgatctgc ctctaattcg gaaatgacag aattttcatg agcaaaaaag cccattctgt
     9901 ctggcattaa atccatttca gtaatgtata tcagtcactc atggaaaaac gtgcacaaaa
     9961 cccgctttct ttcgctttta gggtctctta ctgtgaattg gtagaacaac ttccatacaa
    10021 aaataatgga aaaacgaaca ataaagactt tatttatcaa aaagttaaaa gtggtgtaac
    10081 aactccattc tgacgttgcc gcacaaaccc tataccattc tgtggagtgc atatgagtca
    10141 gggtatcaat aacgatatga ctgccagtaa accgcgccat atcgtcaaaa atctgcgttg
    10201 gtggatactg gtcctttttt tgctcggtgt tactgtcaac tacatcacgc gtaactcgct
    10261 gggtattctc gcccccgaac tgaaaagcag cctagggatt accaccgaac aatattcctg
    10321 gattgtaggt gctttccagc tcgcctatac cctattccaa ccgttgtgcg gatggctgat
    10381 tgacgtcatt ggcctcaaac tgggctttat gatctgcgcg ggactgtggg cactggcctg
    10441 tctgatgcat gcgggggcgg gaaactgggt acatctggcg ctgttgcgct tctttatggg
    10501 cggtgcggaa gccgcggcaa ccccagcaaa cgccaaaacc atcggtgaat ggtttccaaa
    10561 atcagaacgc ccgatcgccg caggctgggc aggcgtcggt ttttccatcg gtgccatgct
    10621 ggccccaccg attatctatt ttgcccacgc ttcattcggc tggcagggtg catttatgtt
    10681 cactggcatc ctggcgatgc tgtgggtggt gctgtggtgg gcgttctatc acagcccgga
    10741 taagcatcct aacctcagca aagccgagct ggactttatt cgccaggata acgaagcgcc
    10801 cccggtcaag ttaccctttc tgaccgcact gaaaaccgtg ggcaaaaata aacgctttta
    10861 cggtatcgct atcccggcct ttatggcaga accggcgtgg gcggtgatga gtttctgggt
    10921 gcctctatac ctgtccaaaa cctatggcat ggacctgaag cagatcgcat tattcgcctg
    10981 gttgccgttc ctcgccgctg atcttggcag tattgccagc ggctatctga ccaaacttta
    11041 cacccgcctg tttgggctga gtcgtgtgaa ctccgttgtc gcatcatcgg tcagtggcgc
    11101 attcttgatg gtttcgctgg cggtgatggc ctttaccaat aacccgtata tcgccatcgt
    11161 ccttatctcc atcggcggct tcggtcacca gatcatctcc tgcatgctga gcgcactggt
    11221 ggtggaatcc ttcgacaaag gtcagatggc cactgttaac ggcatgcgcg gttctgccgc
    11281 gtggatcgca agtttcctgt tctccctgat tatcggggtt accgctgaca aaatcggctt
    11341 taaccccctg tttatcgcca tgggcttctt tgacctgatt ggcgctttct tcctggtcac
    11401 ctttattgct gaacgtcgcg caaaacgcgc ctgagagtgg atgtaaacat gaaaacgctg
    11461 aaaaactgga ccctgcacaa acagtctgcc caccacgttg aactgctggt cgatggccag
    11521 catatcctgt gcctgtacgt gctggaagaa aatctgttcc gcgtgctgtt aaaacgcaaa
    11581 ggcgagctgg cgctagatcg cacctggagc attgctccgg cgcaggatgt gccatgggaa
    11641 gggcgcagcc gcgaagatct cagcggcttt agcctgcccg cgtggacagt gatccagcac
    11701 gacgacaccg tggttatcgc caccgagtca ctgcgtgcaa ccgttcacca gccgctgtgg
    11761 ctggagtggc actatcgtaa caacgaaggc gaatggctgc cgttggtgaa tgaccgcccg
    11821 accagcgctt atctgctgaa tgcccacggc gatggcgtgg cacactatca gagccgtcgt
    11881 aaagatgaac gcttttatgg cctcggagat aaagccggcg atctgcaacg caacggtaaa
    11941 cgttacgaga tgcgtaacct cgacgcgatg ggctacaacg ccgttagtac tgacccgctg
    12001 tacaaacaca ttccattcac catcacccgc cgcgatgaca ttagcttcgg cctgttttat
    12061 gacaacctca gcagctgctg gctggatctg ggcaacgaga tcgacaacta ccacaccgcc
    12121 tatcgccgct ggcaggcgga agcgggtgat atcgactact acctgtttac cggcgaacgc
    12181 gtacttgata tcaccaaagc cttcgtacgc ctgaccggaa aaaccctgtt cggaccgaaa
    12241 tggagcctgg gctacagcgg ttcaactatg cactacaccg atgcgccgga cgcacaaaat
    12301 cagttgatga actttatccg cctgtgtgaa gaacatgcaa ttccctgcga ttcgttccag
    12361 ctctcttccg gttatacctc aattaacggc aaacgctatg tgttcaactg gaactacgac
    12421 aaagtgccgc agccgaaagt gatgagccag gcatttcaca atgctggttt gcacctggcc
    12481 gccaatatca agccgtgcct gttgcaggat cacccacgct ataacgaagt ggcagaaaga
    12541 ggactgttca tccgtgattc agaaacagat gaaccagaac gttccagctt ctgggatgac
    12601 gaaggttcta acctcgactt tactaatccg cagaccatcc agtggtggca ggaaggcgtg
    12661 accacacagc ttctggagat gggtatcgac tccacctgga acgacaacaa cgaattcgaa
    12721 gtatgggacg gtgaagcacg ctgtcacggc tttggtcaga cgatcgcgat caaacatatt
    12781 cgcccagtga tgccactgct gatgatgcga gcctcgatgg aagcccagca gcgtttcgct
    12841 ccggaaaaac gtccgtactt gatctcccgc tccggctgcg cgggaatgca gcgctacgtg
    12901 cagacctgga gtggcgataa ccgcactagc tgggacactc tgcgctacaa cacccgcatg
    12961 ggtctgggga tgagcctctc cgggctgtat aacctcggtc acgacgtcgg cggtttctcc
    13021 ggtgataagc cggacccgga actgtttgta cgctgggtac aaaacggcgt gatgcatccg
    13081 cgctttacga ttcactcatg gaacgatgac cataccgtaa acgaaccgtg gatgtatccg
    13141 ggcgtcactc cggcgatccg cagcgctatt gagttgcgct atcgcctgct gccctacttt
    13201 tataccctga tgtggcaggc atacgccgat gatgaaccaa tgctacgtcc gactttcctt
    13261 gaccacgagc acgacgttca gacttttgaa gagtgtgatg acttcctgct tggtcgcgat
    13321 attttggttg ccagcgtcgt agagcaaggc gagcgccagc gccgtgtatg gctgccagat
    13381 aacgaaactg gctggtacga tttctacaac ggcgaatggt tcagtggcgg tcagtggatc
    13441 accctcgacg caccgctgga gaaactgccg ctgatggttc gtgccggggc tggtctaccg
    13501 ctcagcaaac gcatcaccta tgtgagcgca gaacaagacg atacccgcga actgaaactg
    13561 ttcccgctga aaggtgtggg cacaacgtcc ggcctgctgt ttgaagacga tggcgaaagc
    13621 tggggctacc aaactggtaa tgcgctttgg gtggaatggg aaatggtttg cgatggggcg
    13681 accatcaacc tgaaagtcaa cgcgcgcggg gattatcgtc ctgcatggag tgcactgaaa
    13741 gtgtcattac cattggaaga aaaacgcacg ctgctggtca atggtgttga agggagtgaa
    13801 tggatgcgtt aaatcattac cggggccggg aggcgactgc accttacccg gtccagccat
    13861 cgaatccttt acctcagtat ttcacgctgc aggaaggcag caaacctgta aaccccggga
    13921 gcttaaataa gtaagtgacc ggggtgaaca ggtgaagcca atgcatctgc cgcatgaaag
    13981 acggcgggga attaagcgtc gatcccttta ctacgcaggt aatcttcgta attaccgcta
    14041 aagtcgatcg cgcgttccgg ggtgatttcc agaatgcggg tcgccaggga gcttacgaac
    14101 tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca gttccagcgc catgttcagt
    14161 gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag aatgttcggc
    14221 ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga aagcacttta
    14281 gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct gcgtaccgcc
    14341 tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt cagatcattt
    14401 tcaaactcat attcgtggtc ctgggcgtag taaccaatgc gcgcgttttc agaccatttt
    14461 acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt tgatttaccg
    14521 acgccgttgg tacccagtac cgcaagtttt tcaccgacct ccagcagcag gttgagattt
    14581 ttaaacagtg gaccgttatc aaaccctttg gtcagacctt ccacttccag cgcgttacgg
    14641 aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga ggctttcacc
    14701 tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag
    14761 gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc tttcttcttg
    14821 gcgttatcgg ccagcagacg ttcacgcgcc tgggtcgccg ccgtcatgta ctcatcgtag
    14881 ttacccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt acagaccatg
    14941 ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc gttcagcacc
    15001 tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga
    15061 atatccggat cagcaaacag cgcctgcgcc agaagcacac gcagtttcca gccaggagca
    15121 acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag caacagttca
    15181 ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttaacttc cagatcggcc
    15241 actttatagc cgtcttcctc actcatttcc ggcaaagcat agatgcggtc acgctcctgc
    15301 ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt gaactcttca
    15361 aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag ggaaacgtta
    15421 cccaaagtcg gctctaagtc gccgccgagg atcttcataa aggtggattt accactaccg
    15481 ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat gttttcaaac
    15541 aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg cgtatcctga
    15601 aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc tgctcctcgc
    15661 ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc tgatttcact
    15721 gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa cccaatggcc
    15781 tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat tcgcagcggc
    15841 cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc tgccaaccga
    15901 cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta acactcagcc
    15961 gctggagtat tttgccgcgg agtaccagat ggggctttcc aatatgatgg aagcgaaccc
    16021 gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc agcagctgat
    16081 cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc gtctgtatta
    16141 ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc agttaggcaa
    16201 agatacgcct atcaactgga ccaccaaagt tgagcgtaag aaagcaggcc cgacctggac
    16261 gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgcttc cggctgtcgt
    16321 tccggcaggt ccggataacc cgatggggct gtatgcactc tacatcggtc gcctgtacgc
    16381 tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg
    16441 tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta cccgcgtaca
    16501 gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt atattgaagt
    16561 ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaattg tgccaattac
    16621 cctgacgaag agcgtgcaaa cagtgaccgg tcagccagat gttgaccagg ttgttcttga
    16681 tgaagcgatt aaaaaccgct ccgggatgcc ggttcgtctg aattaatctt caaaacttaa
    16741 agcaaaaggc ggactcataa tccgcctttt ttatttgcca gaccctagtt ggccgggagt
    16801 ataactaaca aaacatatcc caccaacgcc gcccacaaca gcatcggtat tgaatagaga
    16861 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    16921 gatccgggta ataacccaaa gccacctacg tttaccgccc ataccagtaa taaagacggc
    16981 ggcacatagt tcagcaacaa tatggtactc ggcacgttac tgatcacctg cgataaaccg
    17041 attgccgtta accataaccc gggttcagat agatgactca cgttacccaa cacgccttgc
    17101 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    17161 agcgtccagt ccacactgag caccacgcga cgtgccagca gcgcaaagcc tgccgccaca
    17221 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaagac gatatacagc
    17281 cccagacaac tccacaccag ccgcggtttc cactccggtg tttgcacccc cgtatggtat
    17341 tgcagcgcct ttccagggaa acaacaccag cacaggagca ggagcgtcag catcattgcg
    17401 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    17461 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    17521 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    17581 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    17641 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    17701 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    17761 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    17821 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    17881 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    17941 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    18001 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    18061 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    18121 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    18181 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    18241 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    18301 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    18361 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    18421 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tacataatat
    18481 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    18541 tcaggaggtc ttatgaatga gttcaagaag tgtatgcgcg tgtttagtca ttctcccttt
    18601 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    18661 gataaacctt ccgataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    18721 ggcataacgc gttttggtct gaaaaacccc actttttgtc ggatttgcaa tccccttcgc
    18781 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    18841 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    18901 attgaccctt gacgccaggg taagcacatg gcgtttgtta cgatagtggc atatgaattt
    18961 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    19021 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    19081 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    19141 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    19201 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    19261 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    19321 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    19381 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    19441 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    19501 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    19561 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtggtgat
    19621 cggcgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    19681 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    19741 gcagaaatca cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    19801 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    19861 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    19921 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    19981 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    20041 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    20101 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    20161 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    20221 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    20281 tcagcaaaat ttccaggtgc cttttatggt catttccagc gacgataaag cgcatcgggt
    20341 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    20401 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    20461 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    20521 cgacccgaaa ccttaaaaca aaaaatccgc cccgagaggc ggatttttta tatcaccaaa
    20581 gtgattagaa gcggtaacca acaccggcaa tccaggtgcc tacgtcaacg ctacgaatac
    20641 ggctctgctc gtaagagaag tccagagcaa cgttttccat cgggttgaac tgcagaccag
    20701 caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta ggtcgggtat tcagtggtct
    20761 ggaatttacc ataacccacc cccactacgc cgtagatgct tgcccagtcg ttaatgcggt
    20821 aagccggacc agcagtgatg ccgtagtact ggtttttgtt gtagtcacca gagcttgcag
    20881 tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc cagcgggctg ttgtcttctt
    20941 catagcggta tttcaggttg aaaccgccca ttttgttcat ttggccctga gcgtcgctct
    21001 gtgcgtaacc gccagttacg gtagaagtcg cagctacgga agtacctgcg gtgaaagcca
    21061 gaactgcggc cagtgctgaa agacatgcaa tttttttcat aaccacctca aatgtgattc
    21121 aaataagtcc taagttttaa atatatcaaa aattaatggg aaactcttcg cgatttgtga
    21181 tgtctaacgg gccatttcat gtaacagaac gtttccatac accgctatcc atctaaattt
    21241 aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt tttccctgtc attcatccag
    21301 attattccta atcaccagac taatgattcc atcaatcctg gcgcatttta gtcaaaacgg
    21361 gggaaaattt tttcaacaaa tgctcaacca gcattgggta tatccagtac actccacgct
    21421 ttacttaagt ctagatattt gtgggagaaa ggatgcctgg ttcattacgt aaaatgccgg
    21481 tctggttacc aatagtcata ttgctcgttg ccatggcgtc tattcagggt ggagcctcgt
    21541 tagctaagtc actttttcct ctggtgggcg caccgggtgt cactgcgctg cgtctggcat
    21601 taggaacgct gatcctcatc gcgttcttta agccatggcg actgcgcttt gccaaagagc
    21661 aacggttacc gctgttgttt tacggcgttt cgctgggtgg gatgaattat cttttttatc
    21721 tttctattca gacagtaccg ctgggtattg cggtggcgct ggagttcacc ggaccactgg
    21781 cggtggcgct gttctcttct cgtcgcccgg tagatttcgt ctgggttgtg ctggcggttc
    21841 ttggtctgtg gttcctgcta ccgctggggc aagacgtttc ccatgtcgat ttaaccggct
    21901 gtgcgctggc actgggggcc ggggcttgtt gggctattta cattttaagt gggcaacgcg
    21961 caggagcgga acatggccct gcgacggtgg caattggttc gttgattgca gcgttaattt
    22021 tcgtgccaat tggagcgctt caggctggtg aagcactctg gcactggtcg gttattccat
    22081 tgggtctggc tgtcgctatt ctctcgaccg ctctgcctta ttcgctggaa atgattgccc
    22141 tcacccgttt gccaacacgg acatttggta cgctgatgag catggaaccg gcgctggctg
    22201 ccgtttccgg gatgattttc ctcggagaaa cactgacacc catacagctg ctggcgctcg
    22261 gcgctatcat cgccgcttca atggggtcta cgctgacagt acgcaaagag agcaaaataa
    22321 aagaattaga cattaattaa att
//
LOCUS       DAZPJP010000054        21643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPJP010000054 DAZPJP010000000
VERSION     DAZPJP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.054"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHU9J_001889"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHU9J_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1905446.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHU9J_001890"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHU9J_001890"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1905447.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHU9J_001891"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHU9J_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1905448.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHU9J_001892"
     CDS             complement(3717..4511)
                     /locus_tag="ACHU9J_001892"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1905449.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHU9J_001893"
     CDS             4649..4990
                     /locus_tag="ACHU9J_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1905450.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHU9J_001894"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHU9J_001894"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1905451.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHU9J_001895"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHU9J_001895"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1905452.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHU9J_001896"
     CDS             8906..10198
                     /locus_tag="ACHU9J_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1905453.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHU9J_001897"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHU9J_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHU9J_001898"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHU9J_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1905454.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHU9J_001899"
     CDS             complement(10731..11189)
                     /locus_tag="ACHU9J_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1905455.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHU9J_001900"
     CDS             complement(11186..12103)
                     /locus_tag="ACHU9J_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1905456.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHU9J_001901"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHU9J_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1905457.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHU9J_001902"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHU9J_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1905458.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHU9J_001903"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHU9J_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1905459.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHU9J_001904"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHU9J_001904"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1905460.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHU9J_001905"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHU9J_001905"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1905461.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHU9J_001906"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHU9J_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1905462.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHU9J_001907"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHU9J_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR1905463.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHU9J_001908"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHU9J_001908"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1905464.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHU9J_001909"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHU9J_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5013 a         5686 c         5800 g         5144 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZPJP010000055        21501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPJP010000055 DAZPJP010000000
VERSION     DAZPJP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.055"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHU9J_001910"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHU9J_001910"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1905465.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHU9J_001911"
     CDS             complement(2380..2820)
                     /locus_tag="ACHU9J_001911"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1905466.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHU9J_001912"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHU9J_001912"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1905467.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHU9J_001913"
     CDS             3538..4236
                     /locus_tag="ACHU9J_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1905468.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHU9J_001914"
     CDS             4465..5673
                     /locus_tag="ACHU9J_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905469.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHU9J_001915"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHU9J_001915"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1905470.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHU9J_001916"
     tRNA            7780..7856
                     /locus_tag="ACHU9J_001916"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHU9J_001917"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHU9J_001917"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHU9J_001918"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHU9J_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1905471.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHU9J_001919"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHU9J_001919"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1905472.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHU9J_001920"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHU9J_001920"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1905473.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHU9J_001921"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHU9J_001921"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1905474.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHU9J_001922"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHU9J_001922"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1905475.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHU9J_001923"
     CDS             complement(14640..15911)
                     /locus_tag="ACHU9J_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1905476.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16387..16494
                     /locus_tag="ACHU9J_001924"
     CDS             16387..16494
                     /locus_tag="ACHU9J_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1905477.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16870..16977
                     /locus_tag="ACHU9J_001925"
     CDS             16870..16977
                     /locus_tag="ACHU9J_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1905478.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17353..17460
                     /locus_tag="ACHU9J_001926"
     CDS             17353..17460
                     /locus_tag="ACHU9J_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1905479.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17836..17943
                     /locus_tag="ACHU9J_001927"
     CDS             17836..17943
                     /locus_tag="ACHU9J_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1905480.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(18030..19709)
                     /gene="bcsG"
                     /locus_tag="ACHU9J_001928"
     CDS             complement(18030..19709)
                     /gene="bcsG"
                     /locus_tag="ACHU9J_001928"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1905481.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19706..19897)
                     /gene="bcsF"
                     /locus_tag="ACHU9J_001929"
     CDS             complement(19706..19897)
                     /gene="bcsF"
                     /locus_tag="ACHU9J_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1905482.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19894..21465)
                     /gene="bcsE"
                     /locus_tag="ACHU9J_001930"
     CDS             complement(19894..21465)
                     /gene="bcsE"
                     /locus_tag="ACHU9J_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1905483.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         5199 a         5353 c         5780 g         5169 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc
    16441 attgctggca ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc
    16501 atgtgggcgt caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa
    16561 ctctgcggcc tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa
    16621 tgcgcagcac agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag
    16681 cgtcaagccg gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    16741 ttggttgctg gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg
    16801 gggccattct tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca
    16861 ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg
    16921 gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt
    16981 gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcaggggtg
    17041 aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg
    17101 gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat
    17161 cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    17221 gcattggttg ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca
    17281 cgggggccat tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac
    17341 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    17401 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    17461 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    17521 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
    17581 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
    17641 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
    17701 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    17761 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
    17821 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
    17881 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    17941 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    18001 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    18061 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
    18121 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    18181 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
    18241 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    18301 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    18361 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    18421 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    18481 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    18541 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
    18601 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    18661 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    18721 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    18781 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    18841 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    18901 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    18961 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    19021 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    19081 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    19141 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
    19201 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    19261 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    19321 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    19381 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    19441 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
    19501 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
    19561 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
    19621 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    19681 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
    19741 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
    19801 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    19861 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    19921 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    19981 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
    20041 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    20101 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    20161 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    20221 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
    20281 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
    20341 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    20401 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
    20461 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    20521 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    20581 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    20641 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    20701 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    20761 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    20821 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    20881 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    20941 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    21001 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    21061 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
    21121 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
    21181 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    21241 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    21301 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    21361 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    21421 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    21481 gattatcagt ttactagcga a
//
LOCUS       DAZPJP010000056        21011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPJP010000056 DAZPJP010000000
VERSION     DAZPJP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.056"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            211..1593
                     /gene="dosC"
                     /locus_tag="ACHU9J_001931"
     CDS             211..1593
                     /gene="dosC"
                     /locus_tag="ACHU9J_001931"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR1905484.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1618..4017
                     /gene="dosP"
                     /locus_tag="ACHU9J_001932"
                     /gene_synonym="pdeO"
     CDS             1618..4017
                     /gene="dosP"
                     /locus_tag="ACHU9J_001932"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR1905485.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4276..4857
                     /gene="ddpX"
                     /locus_tag="ACHU9J_001933"
     CDS             4276..4857
                     /gene="ddpX"
                     /locus_tag="ACHU9J_001933"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1905486.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4871..6421
                     /locus_tag="ACHU9J_001934"
     CDS             4871..6421
                     /locus_tag="ACHU9J_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1905487.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6423..7445
                     /locus_tag="ACHU9J_001935"
     CDS             6423..7445
                     /locus_tag="ACHU9J_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1905488.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7442..8337
                     /gene="ddpC"
                     /locus_tag="ACHU9J_001936"
                     /pseudo
     CDS             7442..8337
                     /gene="ddpC"
                     /locus_tag="ACHU9J_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
     gene            8334..9320
                     /locus_tag="ACHU9J_001937"
     CDS             8334..9320
                     /locus_tag="ACHU9J_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1905489.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            9349..10245
                     /locus_tag="ACHU9J_001938"
     CDS             9349..10245
                     /locus_tag="ACHU9J_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1905490.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(10301..10732)
                     /gene="osmC"
                     /locus_tag="ACHU9J_001939"
     CDS             complement(10301..10732)
                     /gene="osmC"
                     /locus_tag="ACHU9J_001939"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1905491.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            11077..11292
                     /gene="bdm"
                     /locus_tag="ACHU9J_001940"
     CDS             11077..11292
                     /gene="bdm"
                     /locus_tag="ACHU9J_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1905492.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11394..11531
                     /gene="sra"
                     /locus_tag="ACHU9J_001941"
     CDS             11394..11531
                     /gene="sra"
                     /locus_tag="ACHU9J_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1905493.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            11688..13385
                     /gene="maeA"
                     /locus_tag="ACHU9J_001942"
     CDS             11688..13385
                     /gene="maeA"
                     /locus_tag="ACHU9J_001942"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1905494.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            13519..14521
                     /gene="adhP"
                     /locus_tag="ACHU9J_001943"
                     /pseudo
     CDS             13519..14521
                     /gene="adhP"
                     /locus_tag="ACHU9J_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            14667..14951
                     /locus_tag="ACHU9J_001944"
     CDS             14667..14951
                     /locus_tag="ACHU9J_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1905495.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(15179..15832)
                     /gene="fdnI"
                     /locus_tag="ACHU9J_001945"
     CDS             complement(15179..15832)
                     /gene="fdnI"
                     /locus_tag="ACHU9J_001945"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1905496.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(15825..16709)
                     /gene="fdnH"
                     /locus_tag="ACHU9J_001946"
     CDS             complement(15825..16709)
                     /gene="fdnH"
                     /locus_tag="ACHU9J_001946"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1905497.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(16722..19769)
                     /gene="fdnG"
                     /locus_tag="ACHU9J_001947"
     CDS             complement(16722..19769)
                     /gene="fdnG"
                     /locus_tag="ACHU9J_001947"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(19182..19184),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1905498.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            20001..20882
                     /gene="yddG"
                     /locus_tag="ACHU9J_001948"
     CDS             20001..20882
                     /gene="yddG"
                     /locus_tag="ACHU9J_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1905499.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5162 a         5342 c         5336 g         5171 t
ORIGIN      
        1 tgaaaaacca tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct
       61 gaagaacagt agcgtagatt gaaactgtcc taagtcgctt atttcttttc agtatatctt
      121 catatttcag gagaatatgg tgcagttagt caatctcgca cgttgcgttt tgattccgtc
      181 tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag aatgaaagat
      241 gagtggaccg gacttgtcga acaggcagat ccactcattc gtgctaaagc cgcggaaatt
      301 gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg catcgacccg
      361 catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag tgcgatggaa
      421 cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct aatacaaatc
      481 cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat tgtcgagatg
      541 gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga ttattccgcc
      601 gcagaaaaac ttcaggtcta tcatttctcg attaacagta ttgatatcgc gatggaagtg
      661 atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga aaattatcgt
      721 atcttctcgt tactggaaaa cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt
      781 tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg cagtagtttg
      841 ccattaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca ttattttagt
      901 ggcattgccg aagtaggcca tatctcccgt ctcattcagg atttcgacgg tattttcaat
      961 caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa atttttatta
     1021 cagataagaa ataccgtatc gcagattatt accttgctgc gtgaattgtt tgaagaagta
     1081 tcgcgccacg aagtcggtat ggatgtacta acgaaattac ttaaccgccg tttcctgccg
     1141 actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg
     1201 attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat
     1261 gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccacagtag tgattatgtt
     1321 tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga aaacgaaacg
     1381 ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa agccgcaaac
     1441 ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac
     1501 tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga
     1561 aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg
     1621 aagctaaccg atgcggaaaa tgccgccgat ggcatttttt tccccgccct tgaacaaaat
     1681 atgatgggcg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca
     1741 gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt
     1801 ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga aggtggtaaa
     1861 gcgcgcgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc
     1921 tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg
     1981 gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc
     2041 gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc
     2101 aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg tatgcagccc
     2161 gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt acaacagttg
     2221 ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacacgcac cggtgaaaaa
     2281 atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg
     2341 gtaatgacct tctcggatat caccgaagaa cggcagatcc gccagcttga aggcaatatt
     2401 ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac
     2461 atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa cgggatgccg
     2521 atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg
     2581 accattcgtc agcgtgatgg tgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga
     2641 gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg
     2701 gcgctggaac aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg
     2761 accggtctgc caaatcgcaa taacctgcac aattacctcg atgaccttgt cgacaaagcc
     2821 gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt
     2881 ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc
     2941 aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt gagcctggaa
     3001 aacgacgtca gtaacattac ccaaatcgcc gatgagctac ggaatgtggt cagcaagccg
     3061 ataatgattg acgataaacc attcccgcta accttgagta ttggcatcag ctacgacgtg
     3121 ggtaaaaacc gcgattacct gctctccact gctcacaatg caatggatta tattcgcaag
     3181 aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt
     3241 ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa actggtttac
     3301 cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg
     3361 cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc agaagagatt
     3421 ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa
     3481 tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc gctgcacttt
     3541 cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg tattgacggc
     3601 caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt
     3661 aagcgcattc agatcctgcg cgatatgggc gtgggcttat cggtagatga ttttggcacg
     3721 ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgacaaa
     3781 agttttgtcg atcgttgtct gacggaaaaa cgcatccttg ccttacttga agccattacc
     3841 agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa
     3901 tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc
     3961 ctacccgccg aggaaattcc aggctggatg agcagcgtgt taccgctgaa aatctgacaa
     4021 attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact taaatactgt
     4081 gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca ccaaaactgc
     4141 actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc agcattctgg
     4201 cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat ttttgtctta
     4261 atacaggaac cgtttatgtc ggataccacc gaactggttg atttagccgt gatcttccct
     4321 gatctggaga tcgaattgaa atacgcctgc gctgataaca tcacaggtaa agctatttac
     4381 cagcaagcgc gttgtctgtt acacaaggat gcgattaccg cgctggcgaa aagtatcagt
     4441 atcgcccagc tgtcaggctt acaactggtg atttacgatg cgtatcgccc acaacaagca
     4501 caggcgatgt tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt
     4561 tctaatcaca gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca cgggaacatc
     4621 ctggatatgg gcgcagggtt cgatgaaatg cacgagcgct cccatgccta tcacccttcc
     4681 gtcccgcccg ccgctcagcg caatcgcctg ttgctgaatg cgataatgac tggtggcggt
     4741 tttgtcggta tctccagcga atggtggcac ttcgaattac cccaggcagc gagttaccct
     4801 ctgctcgctg atcaattctc ttgttttata tcccctggca cacagcacgt cagttaatca
     4861 ggaaactgtc atgaagagat cgatatcgtt tcgtcccaca ttgctcgcgc tcgtccttgc
     4921 cacaactttc ccggttgcgc acgccgccgt accgaaagat atgctggtga ttggtaaggc
     4981 cgccgatcca caaaccctcg acccggcggt aacaatagat aataacgact ggacagtgac
     5041 ctacccgtct tatcagcggc tggttcagta caaaacggac ggtgataaag gctcaaccga
     5101 cgttgaaggc gatctggcaa gtagctggaa agcgtctgac gatcaaaaag agtggacgtt
     5161 caccctgaag gacaatgcta aatttgccga tggcacacct gtcactgccg aagcagtaaa
     5221 actctctttt gagcggttac taaaaatcgg ccaggggcca gcagaagcat ttcccaaaga
     5281 tttaaagatt gatactcccg acgaacatac ggtgaagttt acccttagcc aaccattcgc
     5341 accgttcctc tacacgctag cgaatgacgg tgcatccatt atcaatccgg cggtcttaaa
     5401 ggaacatgca gcggatgatg ctcgcggctt cctcgcgcaa aataccgccg gttccggacc
     5461 atttatgctg aaaagctggc aaaaaggtca gcaattagtt ctggtgccaa atccgcatta
     5521 ccccggcaat aaaccgaact ttaagcgagt atcggtaaaa attatcggtg aaagtgcctc
     5581 ccgtcgcctg cagctctccc gtggcgacat tgacattgcc gatgcgctgc cggtggatca
     5641 actcaacgcc ctgaagcagg aaaataaagt caatgtggca gagtatccgt cactgcgcgt
     5701 tacctatctg tatctgaata acagcaaagc gcctcttaat caggcggatc tgcgtcgggc
     5761 catttcctgg tctaccgatt atcagggcat ggttaacggc attctgagtg gtaacggaaa
     5821 acagatgcgc ggcccgattc cggaaggcat gtggggctac gatgcgacgg caatgcaata
     5881 caaccatgac gaaacgaaag ccaaagccga atgggataaa gtgacgagca aacccaccag
     5941 cctgacgttt ctctactccg ataacgatcc gaactgggag cctattgctc tggcgacaca
     6001 atccagtctc aacaagctgg gcatcaatgt gaagctggaa aagctggcga acgccaccat
     6061 gcgcgacaga gtgggtaaag gtgattacga cattgcgatt ggcaactgga gtccggattt
     6121 tgccgacccg tatatgttta tgaattactg gtttgagtca gacaaaaaag gtctgccggg
     6181 taaccgctcg ttctatgaaa acagtgaggt cgataagtta ctgcgcaatg cgcttgcgac
     6241 caccgaccag acgcagcgta cccgggacta ccagcaggca cagaaaatcg tcattgatga
     6301 cgctgcttat gtgtacctgt tccagaaaaa ctaccaactg gcgatgaaca aagaggtgaa
     6361 aggctttgtg ttcaatccca tgctggaaca ggtcttcaat atcaatacca tgagtaaata
     6421 acgtgacctt ctggagtatt ttacgccaac gctgctgggg gctggtgctc gtggtggcgg
     6481 gcgtctgcgt gattacgttt attatctcgc atctgatccc tggcgatccg gcgcggttac
     6541 tggcgggtga ccgcgccagc gatgctatcg tggaaaatat tcgccagcaa ctgggactgg
     6601 accagccact gtatgtacag ttttaccgct acttcagcga cctgtttcat ggtgacctgg
     6661 gaacatccat tcgtaccggg cgtccggtgc tggaagagtt gcgtatattt ttcccggcga
     6721 cgctggaact ggctttttgt gccctgctgc tggcactcct gattggcatc ccgctgggca
     6781 tactctctgc agtctggcga aatcgctggc tggatcatct ggtgcgaata atggccatta
     6841 ccggaatctc cacacctgcg ttctggcttg gactgggcgt cattgtgctg ttttatggtc
     6901 atctgcaaat tcttcccggc ggcggaaggc ttgatgactg gctggatcca ccaacgcacg
     6961 ttaccggctt ttatctgctc gatgcgctgc ttgaaggcaa cggtgaagtc ttcttcaatg
     7021 cgttgcaaca tctcatctta ccggcattaa cgctggcgtt cgttcacctg ggaattgtcg
     7081 ctcgccagat ccgctcagcg atgctggaac aattgagtga agactacatt cgtaccgccc
     7141 gggccagcgg cttgcccggc tggtatatcg ttttatgtta tgcgctaccc aatgcgttga
     7201 tcccatcgat taccgtattg ggtctggcgc tgggcgattt gttgtatggc gcagtgctca
     7261 ccgaaaccgt ttttgcctgg cccggaatgg gtgcatgggt agtaacatca atacaggcgc
     7321 tcgacttccc ggcagtgatg ggctttgccg tcgtggtttc atttgcttat gtgctggtca
     7381 acctggtggt ggatttgctc tatttgtgga ttgatccgcg tatcggacgt ggaggtggtg
     7441 aatgatgcta agcgaggaaa cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg
     7501 tgcaaaactg gtatggatgc tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat
     7561 tgtttaatgc tattgatgat gattttttca ccgtggcttg cgacgcatga tcccaacgcc
     7621 attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg caccgatgaa
     7681 gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat tctcgccgga
     7741 ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct atccggtgtg
     7801 cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct gtcgattcct
     7861 tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt taacgccatg
     7921 ctggcgattg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg gcaagcatta
     7981 gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc gcgttggcat
     8041 ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca ggcatcgctg
     8101 gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct gggtgctcaa
     8161 caaccgagtg ctgaatgggg cgcgatggta gctaatggtc gcaactatgt actcgatcag
     8221 tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgtcgg gtttaatctc
     8281 tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca gtcatgaccc
     8341 aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac ggttatgttc
     8401 acgtgctcaa caatgtgtcc ttgcagatta accgcggtga aattgtcggt ctggtgggag
     8461 aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta ccgacgggca
     8521 gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt aacgctcggg
     8581 agaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag gaaccgatga
     8641 ccgccctcaa tccgacacgt cgaataggtc ttcagatgat ggacgtgatc cgccatcatc
     8701 aaccaataag tcgtcgggaa gccagagcta aagcgattac cctgctggaa gagatgcaaa
     8761 tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt ggtatgcgcc
     8821 agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt gccgacgaac
     8881 cgactacggc gctggacgtc acggtacagt tgcaggtact gcgtttgctt aaacataaag
     8941 ctcgcgccag tggaactgcg gtactgttca tcagccatga tatggccgtg gtgtcgcaac
     9001 tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc gtgacggcag
     9061 acgttatcca tcatccccgg catccgtata ccattggttt gctgcaatgc gcaccagaac
     9121 atggagtacc acgccagcca ttacccgcca ttccagggac ggtaccaaac ctcacccatt
     9181 tgcctgacgg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag tgtgaaaacg
     9241 tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg tatccgcagc
     9301 aggaggtcat aagtatctga cacgttatta acgttacgcg acgtccatat caatttcccg
     9361 gcccgtaaaa gctggcttgg taaaactacg gaacatgttc atgcgattaa tggtattgat
     9421 ttacagatcc gccgtggtga aaccttaggg atcgtcggtg agtcaggctg cggcaaaagc
     9481 accctcgcac agcttttaat gggtatgctg caaccgagcc acgggcaata catccgttca
     9541 ggatcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct taatccgcgc
     9601 ttatcggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag tagtgaacaa
     9661 cagcggcgag cgttggcaaa ggagttggct gtgcaggtgg gtattcgtcc ggagtatctc
     9721 gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat tgccattgcc
     9781 agagcactct cttcgcagcc tgacgtgatt gtgcttgatg agccaacctc ggcgctggat
     9841 atctccgtgc aggcgcagat cctcaattta ctggtaacgc tacaggaaaa tcacgggctg
     9901 acttatgtgc tgatttcaca caatgtctcg gtgatacgtc atatgagcga tcgggtggcg
     9961 gtgatgtatc tcgggcagat tgtagaactg ggcgacgcgc agcaggtgct gacggcacct
    10021 gcacatccat acacccgatt attgctggat tccctccccg ccattgataa accgctggag
    10081 gaagaatggg cattatgtaa aacggatctg ccgggaaacc gcacgttgcc acaaggctgt
    10141 tttttctacg aacgttgccc tctggcaacc cacggatgtg aagtccggca atcattagca
    10201 ataagggagg acggacgtga gctccggtgc tggcgggcgc tgtagagatg ctaacaacgc
    10261 atcaggcatt cacgcgcctg acgcgtcatc cggcaatgct ttacgatttc aactggtaat
    10321 ccagcgtaat ttccgctttc agtacctgag aaaccgggca tcctgctttt gctttctgga
    10381 tgatgccgtc aaaggtagag gcatcaatac ccggcaccgc aacttcactc ttcagtgcga
    10441 ttttcgtaat cgcaaaatcg gcatccactt tatccagcga cacatcggcg gtggtatcaa
    10501 tcgatgttgg cgtgaatccc gcttccccca gcattaatga aagcgccatt gagaaacatg
    10561 cggcatgcgc tgcgccaatc agttcttcag ggttggttcc tttttcgcct tcaaaacgcg
    10621 tgttaaatcc atacggctgt tggttcagca cgccactctg ggtggatact gttcccttcc
    10681 cgcgtttgat atcgccttcc cagtgtgcct gacctttctt atggattgtc attgttgctc
    10741 tcctgtgggc ttaaaaagga aattatagac tacgtgagaa acagcacgag gataagcagg
    10801 atattccgaa taaaatacgg cgaaaatatt acttctgcac ttaaattaaa ttcccacaat
    10861 agataaccgg gaatatatat ctcaagagat attgcatcaa tatcaatcaa ctcaccacaa
    10921 taataataca tatcaatatc atggagttaa aatataaaaa tcatcaacca ggactaatct
    10981 taacaacgaa gcggcaaata tttgccatgc tgaatgtgct ttataaatct gcgatccgta
    11041 gcagacacca taaatacaca gacacggaga atcactatgt ttacttatta tcaggcagaa
    11101 aattcaacag cagaacccgc tctggttaat gccattgagc aaggccttcg ggcagagcaa
    11161 ggtgttgtca ctgaagatga cattctcatg gagctgacca aatgggtgga agcttctgat
    11221 aacgacatcc tcagtgatat ctaccagcaa acgatcaatt atgtggtcag tggccagcac
    11281 cctacgcttt aaggtgctat gcttgatcgg caacctaatt taggggttta gcacgtgttt
    11341 cttcgctacg gcgatgttgt ccttaaaact agcaacagga ttgaggagtt aaaatgaaat
    11401 cgaaccgtca ggcacgtcat attcttggac tggaccataa aatttctaac cagcgcaaaa
    11461 tagttaccga aggtgacaaa tccagcgtgg taaataaccc aaccggcaga aaacgccccg
    11521 ctgaaaagta attcataacc atcagtcctc aatgacgatt aaacaccatt gcctgcgcaa
    11581 tggtgttttt gtttttatct gctttatact tggggccgac gccctggcgg taaagcaaag
    11641 acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg gaaccaaaaa
    11701 caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg gaatttccgt
    11761 tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac ctgctggggt
    11821 tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg atccagtatc
    11881 agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag gacaccaacg
    11941 aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct gttatttata
    12001 ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt tcacgcggcg
    12061 tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac gtaccgaacc
    12121 ataatatcaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt ggtgaccagg
    12181 gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc tgtggcggca
    12241 tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac caacaactgc
    12301 ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgac gaatactatg
    12361 aattcgttga tgaatttatc caggctgtga aacaacgctg gccagacgtg ctgttgcagt
    12421 ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc aatgaaattt
    12481 gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca ctgatcgcag
    12541 caagccgagc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt ggcgcaggtt
    12601 cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa ggattaagcg
    12661 aggaagcggc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg acggacaaga
    12721 tgccgaacct gctgcctttc cagaccaaac tggtgcagaa acgcgaaaac ctcagtgact
    12781 gggataccga cagcgatgtg ctgtcactgc tggatgtggt gcgcaatgta aaaccagata
    12841 ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc cgtgagatgc
    12901 ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtca cgcgtggaag
    12961 ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc acgggcagcc
    13021 cgtttaatcc agtggtatgg aaagataaaa tctaccctat cgcccagtgt aacaacgcct
    13081 ttattttccc gggcatcggc ctgggggtta ttgcttccgg cgcgtcacgt atcaccgatg
    13141 agatgctgat gtcggcaagt gaaacgcttg cacagtattc gccgctggtg ctgaacggcg
    13201 aaggtctggt actaccggaa ctaaaagata ttcagaaagt ctcccgtgca attgcgtttg
    13261 cggttggcaa aatggcgcag cagcaaggcg tggcggtgaa aacctctgcc gaagctttgc
    13321 aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc cgtacctcca
    13381 tctaagcctg cgcccggtag tgaaggctac cgggcaattt tcctctccct ttttcagatc
    13441 tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat gcagaacatc
    13501 atccgaaaag gaggaactat gaaggctgca gttgttacga aggatcatca tgttgacgtt
    13561 acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat ggagtgttgt
    13621 ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa aaccgacgta
    13681 attctgggcc atgaaggcat cggtgtggtg gcagaagtgg gtccaggtgt cacctcatta
    13741 aaaccaggcg atcgtgccag cgtggcgtgg ttctacgaag gatgcggtca ttgcgaatac
    13801 tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata cagcgttgat
    13861 ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa gtgccagatg gtctggactc
    13921 ggcggcggcc agcagcatta cctgtgcggg ggtcaccacc tacaaagccg ttaagctgtc
    13981 aaaaattcgt cccgggcagt ggattgctat ctacggtctt ggcggtctgg gtaacctcgc
    14041 cctgcaatac gcaaagaatg tctttaacgc caaagtgatc gccattgatg tcaatgatga
    14101 gcagttaaaa ctggcaaccg aaatgggtgc agatttagcg attaactcac gcaccgaaga
    14161 cgccgccaaa attgtgcagg agaaaaccgg tggcgctcac tctgcggtgg taacagcagt
    14221 agctaaagct gcgtttaact cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc
    14281 tgtcggtctg ccgccggagt ctatgagcct ggatatccca cgtcttgtgc tggatggcat
    14341 tgaagtggtc ggttcgctgg tcggcacgcg ccaggatcta accgaagcct tccagtttgc
    14401 cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat
    14461 ctttaccgag atggaagaag gcaaaatccg tggccgtatg gtgattgatt tccgtcacta
    14521 agaggtcttt gctgcgactc ccatgttcgg gtcgcagcat cgcacactct ccaacatgaa
    14581 atggctaaat ggattattgt tgcgtaggaa ggaacaataa tgatagtgtt cccagagaag
    14641 gaacgctact gagagtaaag acactaatga aaatggccaa tcatccccgc ccgggggaca
    14701 ttattcagga atcactggac gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg
    14761 aaattgcgcc ctcaacggca agccgattgc tgaccggaaa agcagctttg acgccagaaa
    14821 tggcaattaa actttccgtg gtgatcggca gttcgccgca aatgtggctg aatctgcaaa
    14881 acgcctggag tctggcagag gcagaaaaaa cggtggatgt gtcgcgatta cgccgtctgg
    14941 ttacgcaata aaaaatggca ccttataaag cgccatttga aactgatgac aaacgcaaca
    15001 ttgcctgatg cgctacgctt atcaggccta cacgttcact tcaatctatt gtatttgaag
    15061 ggttttcatg ccggataagg cattcacgac gcatccggga tgaacaaagc ccacgttgtc
    15121 tacaatatga aaatggcgcc ttttacagcg ccattttcag cttaaagttc aaaagagatt
    15181 atatcccttc ttcactctct tttttcgctt ctgccttctc gatttcacga taccagcgcg
    15241 gatggtgttt cttcgcccag cgacggctca ccttcccttc gatcatccct ttaatcgacc
    15301 ctttcaccca gaatgccata tacatatgga tcaggatggc gtggatcagg atgatacccg
    15361 cagccgcgtg gatcagcagg ctgtagcgaa caacctgcat cgggaagtac tgcgcaaagt
    15421 acggacgcca gataatcacc ccggtcacca gcagcacgaa aatcatgctc atgatcgacc
    15481 agaacatcat cttttgcccg gcgttgtact taccaacatc cgccactttg tgctcattgc
    15541 cttttaatac ttcgacaatg ttcaacagcc acggaatatc tttcttatcc gggatgttgt
    15601 gatgcacaaa acgcacaaac ataaacatca gtgcgacgaa aatcgcaatg ccgaagaacg
    15661 ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg cgtcagccat tgcagcgtcg
    15721 ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa gcaaatcacc acggtccagt
    15781 gacaggcgcg atcaataaat ttggtgcgca caatcatttt cgacttactc atgatgatcc
    15841 tcctcgtcat cgtccacttc cttattcggg ccaataccga tgtagtggaa aatcaacccg
    15901 gcaaaagtgg caataaagcc agccgctgcc agcggtttca acgcgccttt ccacaggctt
    15961 accgaggtgt cgatcttcgg atctttcggc agaccgtaat acagctccgg ctgatcggcg
    16021 tgatgcagca cgtacataac gtgcgtacca ccgacccctt tcgggttgta gacgccagca
    16081 tgttcgtaac cacgcgcttt cagtttcgcc acgcgctctt ccgccagctc cagcatctcc
    16141 ttcttggtgc cgaagtggat agccccggtc ggacaggttt tcacacaagc cggttcctgg
    16201 ccgacgctga cgcgatcgac gcagagcgtg catttatata cccggttatc ctctttgttg
    16261 aggcgcggaa tattaaacgg acacccggca atgcagtaac cacagccgat gcagttttcc
    16321 gactggaaat cgacaatccc gttagcgtac tgaatgattg caccagcaga cgggcacgcc
    16381 ttcaggcagc cgggatcttc acagtgcata cagccgtctt tacggatcag ccactccagc
    16441 ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc aggacttggc gctcagatcg
    16501 gcggggttat cgtaaacccc gacgcagtgc cccacttcat cacggatgtc gttccactcc
    16561 gaacacgcca cctgacaggc tttacagccg atacaggtgg aaacgtcgat aagttttgcg
    16621 acttctgctt tgtaatcacg cacctgagaa ggcggcgtga tggagttagt tgcggacctt
    16681 ttgataatat cctgcgtttc catagccatc tgttcgcccc cttacgcctt ctcgatgttg
    16741 actaagaacg ctttatattc cggcgtttgc gagtttgcat caccgacatt cggcgtcaga
    16801 gtgttagcga tataaccttt acgcgcgaca ccctcaaagc cccagtggat tggaataccc
    16861 accgtttcaa cctgctgacc gtttacattc agcggtttca gacggcgcgt taccaccgcc
    16921 accgcgcgga taaagccacg cttgcttgag acagtgacac gatcgccatt attaatgcct
    16981 ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt ccggctgagc aattgcgttg
    17041 agcaatgcgt gcttggtcca ggtgtggaag tgctcggtca gacgataggt cgtacccaca
    17101 tacgggaact gctctttttt acccatccgc agcgcgtctt gttcatacag acgaacaacc
    17161 gggttagaca ccacgttcgg atgcagcggg ttagtgccca gcggcgtttc aatcggctcg
    17221 tagtgttccg ggaacggacc ttccgccatt ttgttgatgg caaacaggcg tcccatccct
    17281 tccggctgca tgataaacgg cccggttggc gtacccggtg cggcattgcc gaagtccgga
    17341 atatcgttac ccgtccactt gctgccgttc cactggatca gcatccgttt cggatcccac
    17401 ggtttaccat tgatatccgc cgaagcacgg ttgtagagca cgcgacggtt gagcggccac
    17461 gcccaggccc atcccagcgt attccccaga ccggatgggt cggagttatc gcggttagcc
    17521 atctggttgc cctgctctgt ccagctaccg gtgtagatcc agcaagaaga tgcggttgta
    17581 ccgtcatcac gcagatgcgc aaagctactc agcaactgac ctttcttcgc aatcagcacg
    17641 ccattagcgt catagagatc ttccagcgca tagccgttat tctctttcgc cacttcgtca
    17701 gattgcggtt cgtgcggctg cttgtagttc cagctcatct tcatcagcgg ttctacgcct
    17761 ttaccacctt cggcctggta cagctcgcgc agatgatggt agatacccgc cagaatttcg
    17821 ccgtcgttac gcgcttcgcc cggcgcgtcc tgacctttcc agtgccactg caaccagcga
    17881 ccggagttag cgatagaacc atcttcttcg gcaaagcagg tcgaaggcag acggaatact
    17941 tcagtctgaa tagacgccgg atcgacatcg ttcgactcgc cgtggttctg ccagaaggta
    18001 gaggtttcag tcaccagcgg atcgataacc accatgtact tcagcttgct caggcagctc
    18061 accactttgt ttttgtccgg gaaggacgca accgggttaa agccctggca gaagtaaccg
    18121 gtgactttgc cttcgtccat catgttgaaa tacttgatga cgtcgtaggt ctggtcccac
    18181 ttcggcagcc agtcatagcc ccagttgttc tctttctgcg cggcatcgcc atagaaagat
    18241 ttcatcaggc taacgaagaa cttcggatag ttgctccagt agttcacctg atcagccagc
    18301 gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat caacctgttt ttctgacgga
    18361 agcgtcagat aacctggcag gctggtagag agcagaccta agtcagtcag cccctgaatg
    18421 ttggagtgac cacgcaatgc gttcacgccg ccaccggcca tacccatgtt accgagcagc
    18481 aactggatca tcgccatagt acggatgttc tgcgcaccca cggtgtgctg cgtccagccc
    18541 agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag cacttcacac
    18601 actttcagga agtcggcttt tggcgtaccg cagatgtttt caacgacgtc cggcgtgtag
    18661 cgggaaacgt gcgctttcag caggttccac acacagcgcg gatgagtcag tgtttcatcg
    18721 cgttttgcat agccgttttc atcgaactga tagttccagg acgacttatc atactggcgt
    18781 ttttcagcgt cgtagccgct gaacaggccg tcttcgaaag caaaatcatc acgcaccagc
    18841 aggctggcgt tggtgtaatg tttaacgtat tcggcgttga ttttgttgtt ttcgatcagg
    18901 tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg aacgaatagg tgcgtaaata
    18961 tccgccacag aagcggtacg cgtaaaacga ggatcgacaa cgatcaaggt tgcatcgttg
    19021 ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc agcgttaccg
    19081 cccattacca ttacgacgtt agcgtttttg atatccaccc agtggttggt catcgcaccg
    19141 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacgcg cgcctggttg
    19201 tctaccgcca gcatcccgag ggagcgggca aatttctggg tcagcatccc ggtttcgttg
    19261 ctggcaccgg aggcacacag cataccggta gaaagccaac ggtttaccgt tacgccctgc
    19321 tcgttctttt caataaagtt agcgtcacgg tcagctttca tcagcttcgc aatacgggag
    19381 aatgcttctt cccagctaat gcgctgccat ttgtcagaac ctggcgcacg atattccggg
    19441 tagcgcagac ggttttcgct gttgacgtaa tccagcaaac cggccccttt tgggcacagc
    19501 gcaccacggc ttaccggatg atccgggtca ccttcaatgt gataaatcgc ttctctggcg
    19561 tttttcgcgc catcacccag gctatacatc aatagcccgc aacctacgga acagtatgtg
    19621 caggtgttac ggatctcttt agcgcgtaat aatttgtagt ttcgcgcctg agccagtgct
    19681 tgcttcgggg caaagcccaa tgccgctacc gttgttccag ccataccgcc cgcgcagatt
    19741 ttaaaaaatt gtctgcgact gacgtccatt gctttcctct tttttcaggg gtattactgc
    19801 gcgaggaaac taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa gaacacgtgt
    19861 atgccacaaa ataatccgcc tgcgcaaatt aacctacctc aatagcggta gaaaaacgca
    19921 ccactgcctg acaggccagt taaaaaaatg ctataaaatt cagcttaatt tttaacggca
    19981 agagagacaa aacagcgagc atgacacgac aaaaagcaac gctcataggg ctaatagcga
    20041 tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt cagcgagggg ctcggtccgg
    20101 tcggcggcgc agctgctatc tattcattaa gcgggctgct gttaatcttc acggttggat
    20161 ttccgcgtat tcggcagatt ccgaaaggct atttactcgc cgggagtctg ttattcgtca
    20221 gctatgaaat ctgtctggcg ctttccttag ggtatgcggc gacccgtcat caggcgattg
    20281 aagtgggtat ggtgaactat ctgtggccca gcctgacaat tctctttgcc attctgttta
    20341 atggtcagaa aaccaactgg ctgattgtac caggattatt attagccctg gtcggcgtct
    20401 gctgggtgtt aggcggtgag aatgggttac attatgatga aatcatcaat aatatcacca
    20461 ccagcccatt gagttatttc ctggcgttta ttggtgcgtt tatctgggca gcctattgca
    20521 cagtaacgaa taaatacgca cgcggattta acggaattac cgtttttgtc ctgctaacgg
    20581 gagcatgtct gtggatttac tattttctca cgccacaacc agaaatggta tttagcacgc
    20641 ccgtcatgat taaactcatc tctgcggcat ttaccttagg atttgcttat gctgcatgga
    20701 atgtcggtat attgcatggc aatgtcacca taatggcggt aggctcgtat tttacgcctg
    20761 tactttcctc agcgcttgca gccgtgctgc tcagcgcccc gctgtcgttc tcgttctggc
    20821 aaggcgcgct aatggtctgc ggcggttccc tgctctgctg gctggcgaca cgtcgtggtt
    20881 aaaataattg ccgggtctac ggcccggcag ttaatcagat aaattccgac aatattaaaa
    20941 aataaatttc aaataccagt aattcacctt atatctcatt tcgacttcga tcacatatcg
    21001 ataaataata t
//
LOCUS       DAZPJP010000057        21003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPJP010000057 DAZPJP010000000
VERSION     DAZPJP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.057"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHU9J_001949"
     CDS             complement(<1..170)
                     /locus_tag="ACHU9J_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR1905500.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHU9J_001950"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHU9J_001950"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1905501.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHU9J_001951"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHU9J_001951"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1905502.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHU9J_001952"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHU9J_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1905503.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHU9J_001953"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHU9J_001953"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1905504.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHU9J_001954"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHU9J_001954"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1905505.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHU9J_001955"
     CDS             4288..5151
                     /locus_tag="ACHU9J_001955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1905506.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDVIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHU9J_001956"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHU9J_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1905507.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHU9J_001957"
     CDS             6489..7106
                     /locus_tag="ACHU9J_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1905508.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHU9J_001958"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHU9J_001958"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1905509.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHU9J_001959"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHU9J_001959"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1905510.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHU9J_001960"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHU9J_001960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1905511.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHU9J_001961"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHU9J_001961"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1905512.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHU9J_001962"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHU9J_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1905513.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHU9J_001963"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHU9J_001963"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1905514.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHU9J_001964"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHU9J_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1905515.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="ACHU9J_001965"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="ACHU9J_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1905516.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="ACHU9J_001966"
     CDS             18072..19781
                     /locus_tag="ACHU9J_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1905517.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHU9J_001967"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHU9J_001967"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1905518.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5716 c         5385 g         4819 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg tgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat cttgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcacc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAZPJP010000058        20593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPJP010000058 DAZPJP010000000
VERSION     DAZPJP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.058"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHU9J_001968"
     CDS             <1..706
                     /locus_tag="ACHU9J_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1905519.1"
                     /translation="NKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
     gene            703..2154
                     /gene="djlC"
                     /locus_tag="ACHU9J_001969"
     CDS             703..2154
                     /gene="djlC"
                     /locus_tag="ACHU9J_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1905520.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2214..3884)
                     /gene="hscC"
                     /locus_tag="ACHU9J_001970"
     CDS             complement(2214..3884)
                     /gene="hscC"
                     /locus_tag="ACHU9J_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1905521.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(3976..4911)
                     /gene="rihA"
                     /locus_tag="ACHU9J_001971"
     CDS             complement(3976..4911)
                     /gene="rihA"
                     /locus_tag="ACHU9J_001971"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1905522.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5029..5754)
                     /gene="gltL"
                     /locus_tag="ACHU9J_001972"
     CDS             complement(5029..5754)
                     /gene="gltL"
                     /locus_tag="ACHU9J_001972"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1905523.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5754..6428)
                     /gene="gltK"
                     /locus_tag="ACHU9J_001973"
     CDS             complement(5754..6428)
                     /gene="gltK"
                     /locus_tag="ACHU9J_001973"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1905524.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6428..7168)
                     /gene="gltJ"
                     /locus_tag="ACHU9J_001974"
     CDS             complement(6428..7168)
                     /gene="gltJ"
                     /locus_tag="ACHU9J_001974"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1905525.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7338..8246)
                     /gene="gltI"
                     /locus_tag="ACHU9J_001975"
     CDS             complement(7338..8246)
                     /gene="gltI"
                     /locus_tag="ACHU9J_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1905526.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8701..10578
                     /locus_tag="ACHU9J_001976"
     CDS             8701..10578
                     /locus_tag="ACHU9J_001976"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1905527.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10656..12194)
                     /gene="lnt"
                     /locus_tag="ACHU9J_001977"
     CDS             complement(10656..12194)
                     /gene="lnt"
                     /locus_tag="ACHU9J_001977"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1905528.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12219..13097)
                     /gene="corC"
                     /locus_tag="ACHU9J_001978"
     CDS             complement(12219..13097)
                     /gene="corC"
                     /locus_tag="ACHU9J_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1905529.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13187..13654)
                     /gene="ybeY"
                     /locus_tag="ACHU9J_001979"
     CDS             complement(13187..13654)
                     /gene="ybeY"
                     /locus_tag="ACHU9J_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1905530.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13651..14691)
                     /locus_tag="ACHU9J_001980"
     CDS             complement(13651..14691)
                     /locus_tag="ACHU9J_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1905531.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14844..16268)
                     /gene="miaB"
                     /locus_tag="ACHU9J_001981"
     CDS             complement(14844..16268)
                     /gene="miaB"
                     /locus_tag="ACHU9J_001981"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1905532.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16414..17589
                     /gene="ubiF"
                     /locus_tag="ACHU9J_001982"
     CDS             16414..17589
                     /gene="ubiF"
                     /locus_tag="ACHU9J_001982"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1905533.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17743..17817)
                     /locus_tag="ACHU9J_001983"
     tRNA            complement(17743..17817)
                     /locus_tag="ACHU9J_001983"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17783..17785),aa:Gln,seq:ctg)
     gene            complement(17866..17942)
                     /locus_tag="ACHU9J_001984"
     tRNA            complement(17866..17942)
                     /locus_tag="ACHU9J_001984"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17906..17908),aa:Met,seq:cat)
     gene            complement(17958..18032)
                     /locus_tag="ACHU9J_001985"
     tRNA            complement(17958..18032)
                     /locus_tag="ACHU9J_001985"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17998..18000),aa:Gln,seq:ttg)
     gene            complement(18067..18141)
                     /locus_tag="ACHU9J_001986"
     tRNA            complement(18067..18141)
                     /locus_tag="ACHU9J_001986"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18107..18109),aa:Gln,seq:ttg)
     gene            complement(18165..18249)
                     /locus_tag="ACHU9J_001987"
     tRNA            complement(18165..18249)
                     /locus_tag="ACHU9J_001987"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18213..18215),aa:Leu,seq:tag)
     gene            complement(18258..18334)
                     /locus_tag="ACHU9J_001988"
     tRNA            complement(18258..18334)
                     /locus_tag="ACHU9J_001988"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18298..18300),aa:Met,seq:cat)
     gene            complement(18714..20378)
                     /gene="asnB"
                     /locus_tag="ACHU9J_001989"
     CDS             complement(18714..20378)
                     /gene="asnB"
                     /locus_tag="ACHU9J_001989"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1905534.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20332..>20593)
                     /locus_tag="ACHU9J_001990"
     CDS             complement(20332..>20593)
                     /locus_tag="ACHU9J_001990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905535.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5004 a         5175 c         5340 g         5074 t
ORIGIN      
        1 gaataaggaa gaacaatatc ttctttttgc gctttctgcg cccatggaaa tacttaatca
       61 gagttgcaaa cccgcgcatg actcgccaaa aatgtatact gggataaaag aattcgatct
      121 ctcatcatcc tggggaataa ataatcgtga tgatttaatt cagactattt atcagatgac
      181 tgatgatggt catgccaatg atttagctgg cttatatctg acatggcaac gctcttcccc
      241 tgaagagtgg aaagcattaa ttgctggtgg ctcggagaga gggttgattt atacccaatt
      301 cgttgcccaa accgctatgt gctgtggaga agggggaatc aaagcctggg attatgttcg
      361 aatggggttt ctgtcacggg ttggtgtgct caacaactgg ctgacggaag aagagagttt
      421 atggctgcaa tcgcgcgtct atgcacgagc tcaccattac tatcacagct ggatgcacta
      481 tttttctgcc tattcactag ggcgtctcta ctggcaatct tctcagtgcg aagacaacgc
      541 ttcgctacgc gaggcgctaa ccctgtacaa atacgatagc gccgggagtc ggatgttcga
      601 agagctggca gcgggaagcg accgtttcta tgccacgctt ccctggcaac cattaactgt
      661 ccagcctgaa tgcccggtaa cgcttaagga tgtgagcgac ctatgaaaac atgttggcaa
      721 atcctcgaaa ttgaaagcac gacgcaaata gacattatcc gccaggctta tcttgctcgc
      781 ttaccgttgt gtcatcccga aaccgatccg caggggttta aagcattacg ccaggcctat
      841 gaagaggcgc tgcgactggc ggtaaatcct gtcgaggaag cagatgatga agaaaaagat
      901 gccaccgctg aacatgaaat actacgtgca ttcaggacat tactggattc agaaagtgat
      961 cgttttcagc cctccgcctg gcagaaattt attcagcaat taaatacctg gaacatggag
     1021 gatgtcgatc aattacgctg gccgctgtgt gcaatcgcta tagaagcgcg atatctttca
     1081 ttaaattgtg cttctttgtt ggcagagcgt ttgaactggc attcgtttaa tgacagcgaa
     1141 ggaatggatg aggaagaaag ggaggctttt cttgaggcca ttcaggctgg tgattgtttc
     1201 gatttcctta gccttctgga atatcccgtc gcgttacaga accagactgt tgaatattac
     1261 ttcgcgctgg aacgttgctg ccgttaccat cctgactatg tcactgcgtt tttggcgatg
     1321 gaaggaccgt ggttcattcc tgatgatgca aagttacatc gcaaactgtt gcgctggtac
     1381 agctcggtgc aaacaggtat ggcggaactc attcctgtag ccaaacagtg gcaagcggaa
     1441 gaaccagaaa gcgaagatgc ccggtattac cagtgtgcac aacgtttgta ctgcggcgag
     1501 ggggaaagcc tgcttgccga tctctgcgcg tactgggaaa gttacccatc tacacaagct
     1561 gataatttgt tgttgcagtg gagcaagcgt cattgcccgg attatttcgc gttattagtg
     1621 atggttatcg aagcgcgaag catggtagat gcgcaaggta aaccgttgaa atatgttcct
     1681 ggtgagagcg cccggacgcg gctgttatgg gcggagattt tacatagcgg aaaattatcg
     1741 ccgttaggtc aatcgtttat tgagtcgtta ttcttcaagc gcaaagcatg ggcgtggtgg
     1801 aaatcgagag tcggtagcga gacagagcaa gattcaccgt tactggattt gtatcgggta
     1861 gcggaacagg ttgtgctgga agcgttcccg aaacaagaga tgctggcccg tcttaataca
     1921 aggctggaag gtggagatgc tcatccatta gaggccatta tcacccagat gctgttgacg
     1981 aaagtgaaac tcgagccgga ggatgaagat gtcgatgagc caacacctga aaaccatgaa
     2041 gaaaaaaatg atgaggatga aaaaccacag agcattacca gtattatcaa aatcagctta
     2101 acggtgctga tgataggtta tgcgctcgga aaaatcgcga tgttgtttag ctgaggatgt
     2161 gttttttgcg ccgatgccgg atgcagcgtt tatgccgcat ccggcagccg tgtttaaggg
     2221 atctcaatag ccaaataatc gctcgcccgt cgacgaactt catcgacgcg ggccatatcg
     2281 tttgactgca acgccgcatc aaaatcggtg atcacccggc caatctcttc tcgctcgtca
     2341 cccagcgccc gcgcccacaa ctcttccagt ttggccttaa aggtgcggtt gattagcata
     2401 tcgcgcggat aaattttcaa tgcggataac cgcgtccgac tctcttcaat ctgctgcgcg
     2461 ctcaatgtta ccgggctatg attaatgact ctggacttaa cgctgccatc ttccagaagc
     2521 acgtcaactt caagcaagcc gttgatatca taactaaagc gaatatcaat cgactgataa
     2581 gccccggttt tcttcaacgg cacatcgaag gattcaacca gaatgttatt tttaactttg
     2641 tggttttctc cctgatagac gttaaccgta atagaatcct gttccgggtg catggtcgag
     2701 taagtttcta cacgcgacac gggtactgtg gtgtttcgtt caataattgg cgagaaaatg
     2761 ccggaaacgc cctggcggtt aacttcaacg cccaacgagt aagggcaaat atcagtgagg
     2821 atcacctctt caatatcttc actgcgtaag cggcaggcgg cctggattgc tgcgcccagc
     2881 gcgacaatgg tgctcgggtc gtaactttga tacggtaatt tgccaaacag acgcacggcg
     2941 attcgctgca ccagcggcat ttgtgacgcg ccgccaacca gcaccagact gtcgatttga
     3001 ctcggcttca gacgtgcatc gcgcaacgcc tgttcaatcg gcactcgcaa gcgattgagc
     3061 agcggcaacc acaaatcttc cagttcgttc tcgtaaaatt cgcactcccg cgtttcatcc
     3121 tggtactgcc agcgaatgtg caatggcgat tgattgctac attttgccgc ttccacacag
     3181 gcgtacaagg ctgccagttc actctcgtta agcgtggtcc tggcgacatc cgcgcgcttt
     3241 aaaacgtcat cgaccaacat atgggtaaaa tcttcgccac cgaggaagtt atcgccagcg
     3301 gaggcgtgaa cttcaatcac cggcgtggcg tattcgagca ccgtgacgtc aaacgtgccg
     3361 ccaccgagat caaaaaccag cgagcgggta ttttgttgcg tatgcaggcc atatgccatc
     3421 gcagctgctg tgggttcatt aattaagcgt accgcattta acccggctaa ctccgctgct
     3481 aaacgggtat gcttgcgttg ttcatcgctg aaataagccg gaacggagat caccacatct
     3541 ttaatcggac gttgcagaaa ttcttcggca tcttctttta atgagcgtaa taccaaagag
     3601 gacagttctg gcgcgttaaa tgtgtcgctg cctaaccgcc agttggtatt actgcccatt
     3661 gcacgtttaa ataacgctgc cgttttatcc ggatgcgaag tacgccgtga tacagccggt
     3721 tttccgacta aaatatgatt attttcatcc atgctaatta tggatggtgt taaatattca
     3781 ccgaacttat ttggaattaa ttgcgcggca ccgtctttcc agacggcaat taaactattg
     3841 gtagtgccga gatcgatacc aatggcgagt tctgcattat ccattgcatt tatatcttga
     3901 aagaaaaaga gataaacaga taaacagatt aagaccaccc aggtaacgcg tggtgatctt
     3961 aatcaatgac gtgtgttaag cgtaaaattt cagacgatca gccagtaaat caacaaagcc
     4021 ctgacgatca acatcgacca ttacagtggc attcggttta ttgccagtca gataataata
     4081 atcaacaacc gtcataccct ggctatattt cccctgtgtt tccacgccaa cccagcgctc
     4141 aacagtggta aacaactcgg gtttcaacag ccaggcgatg gtgcatgggt catgcagcgg
     4201 tgcaccgaca aagccccatt tttcgtcttt atgatattcg aggaagaaat ccagcagttc
     4261 ggcaacaatg gttgaaacag ggttgccaat cgcgcggaaa cgctcggtgt cttcaacgtg
     4321 gatttgtgct ttgtgagtaa catccagacc ggccatcacc actgggatcc ctgactggaa
     4381 gacaatttct gccgcttccg ggtcaacgta aatattaaat tcagccgcag gcgtccagtt
     4441 accaagcccc attgcgccgc ccataatcac gatacgggca attttgctat gcagttccgg
     4501 atggctattg agcagcaagg caacgttagt ttgcggtccg gtagacacaa tcgtgacagg
     4561 ttccgcactt tcgcgcagcg ttttcgccat cagctctacc gccgtacagt tttgcggtgc
     4621 gaacgtcggt tccggtaatg ccgggccatc cagaccgctt tcgccgtgca cattgtccgc
     4681 gataatcaac tcacgcatta acggttttac cgcgccgcct gctaccggaa tatcggtgcg
     4741 attaagcaag gtcagcatac gcagaacatt gcgtaaggtt ttttctggtg tctggtttcc
     4801 ggcggaagac gtaattgctt tgacatcaag ctctggtgag gcgagggcga gaactattgc
     4861 gatagcgtcg tcatgacctg ggtcgcaatc taacagaatt ggcagtgcca ttgttgctcc
     4921 ttgttgtgtg cttctttgcg acaagggtaa cgccaggatg taacagatac gaggggcgaa
     4981 acgataaagc gtgagatggc gcgcacttgg gtatgcgcgc cagagtgatt aatgcaggat
     5041 tttcgcgagg aagtctttcg cgcggtccga tttcggatca tcgaagaaag cgtctttcgg
     5101 cgagtcttcg acaattttac cctcgtccat aaagatcacc cgattcgcca ctttgcgggc
     5161 aaagcccatt tcgtgggtca ccaccatcat ggtcattcct tcgttcgcca gttccaccat
     5221 cacgtccagc acttcgttga tcatctccgg atccagcgcc gatgtcggtt cgtcgaacag
     5281 catcgcaata ggatccatac acaatgcgcg agcgattgcc acacgctgct gctgaccgcc
     5341 ggaaagctgc gccggaaatt tattggcatg tgcggaaagt cccacgcgct ccagcaattt
     5401 caatgctttt tcacgagcgg gcgctttatc gcgtttaagc actttcacct gcgccagggt
     5461 caggttttcg ataatcgaca gatgagggaa cagctcgaaa tgctggaata ccatcccgac
     5521 gcgggaacgc agctttgcca gatcggtttt cttgtcatta accacgatac catcgacggt
     5581 gatttcacct tgctgcactg gttcgaggcc gttgacggtt ttaatcagcg ttgatttgcc
     5641 ggaaccagac gggccgcaaa ccaccaccac ttcgcctttt ttcacttcgg ttgagcagtc
     5701 ggtcagcacc tgaaagtgac cataccattt tgaaacattt ttcagggtaa tcattatgct
     5761 gtccttcttt tcaagtagct gaccaacaac gacgcgctaa gactaataac gaaataaaca
     5821 aatccggcaa acaggatcat ctcaacctgc gtaccatcac gctcaccaat ggttgaggcg
     5881 gtacggaaga aatcggccag agataacaca tacaccagtg aggtatcctg gaacagtacg
     5941 atgccctgag tgagcagaag cggcaccatc gcgcggaacg cctgcggcag aataatcagt
     6001 ttcatcgact gccagtgagt cattcccaac gccagcgcgg cgctcgactg accacgagaa
     6061 atactttgaa taccagcacg gataatctct gaatagtagg ccgcttcaaa catcgaaaac
     6121 gccaccatcg ccgaaattaa acggatatca ttttttggtg ataatcccag cacgttttgc
     6181 agaaaacctg gcacgatcag gtaaaaccac agcaaaacca taactaaagg aatcgagcgg
     6241 aatacgttaa cgtaggcttt ggcaaaccac gctacgggcg caaagctgga caagcgcatc
     6301 accgccagca tggtgcccca caaaatacca atcacgactg ccgtgacggt gattttcagc
     6361 gtgatcacca gcccgtcgag cagatatggc agggaaggga caatggaact ccagtcaaac
     6421 tcgtacatta tttgcccccc atgttgccag gcaggcgaac tttacgttca accagcgtca
     6481 ttaccagcat gataaccgcg ttaatcaaca catacgccag cgtaatagcg gtaaacgact
     6541 cccaggcgtg ggctgagtaa tcaagcaatt tacccgcctg cgccgccata tccaccagac
     6601 caatagtcga ggcgatggcg gagtttttca ccaggttcat catctcggaa gtcatcggcg
     6661 ggacgataac gcgataagca ttaggcagca gtacgtatcg ataagcctgc ggtagcgtca
     6721 ggcccatcgc cagcgcggca tttttttgcc cacgcggcaa cgactgaatg gcggcgcgta
     6781 cctgttcgca aacacgggcg gcggtaaaca gccccaggca gagcatggat gaaaggaaaa
     6841 actgaatatt gggatccagc tcggccttaa accacatgcc gattttctcc ggcaacagct
     6901 ccgggatcac cagataccag gtaaagaatt gcacaatcaa cggcacgttg cggaacagtt
     6961 cgacatacaa cgtacccaga ccggagagaa aacggtttgg aacggtacgt aaaatgccga
     7021 aaaatgaacc gacgaggaaa gcgataatcc aggcgcagat cgacaaagcg atcgtcacct
     7081 gaaaaccact ccagatccaa ccaagatagg tggtgttgcc gaacggggct tgttgtaaaa
     7141 aaatacccca gttccagtct atagacataa atctactcca gaaaaaagag ggtagcagcg
     7201 ttaactgcta ccctcgaaga ttgttaccca gcgtattgcg gttttcaggc ccgatgggga
     7261 acgaccatca ggcgtatagt ctgtccgtgc tacgtaacaa tcgagagggc tggaatttcc
     7321 gcccctggtt cttgtaatta gttcagtgcc ttgtcattcg gttctttgaa cagtgctttc
     7381 atttcgtctg acagttcgaa attcatgttc aggtttttcg gcggaatggg atttttgaac
     7441 catttatcaa accatttttc cgcttcaccg gaggtctgca cctgcgcggt ggtgtcatcc
     7501 atcagctttt tgaactgcgg atcgtcttta cgcaacatgc aaccgtaggc ttcctgagac
     7561 tgcggcttgc cgacgatttc ccagttgtct ggtttcttcg ctttcgcacg ttcaccggcc
     7621 agcagggcat catccatcat aaaggcaacg gcacgaccgc tttccagggt gcggaaagag
     7681 tcactgtgat ctttggcgct gatgatgcgc atattcattt tttgctcttc attcagtttg
     7741 ttgagcaaaa cttcagaggt agtgccggaa gtgacgacta cggctttgtc tttcaggtcg
     7801 gcaaaatctt tgatatcgcc gccctttttg gtcagcaggc gcgtaccgac cacgaaaata
     7861 gtatcagaga aagccgcctg tttttggcgt tcgacgttgt tggtggtaga accacattca
     7921 aaatcgaaag tgccgttttg cagcagtgga atacggtttt gtgaggtaat cggaatcagt
     7981 tttacctgca agtccggttt gttgagtttc tttttcactg cttcaacaat ggcgttggag
     8041 taatcctgcg agtaacccac cactttttgc tgattgtcgt aataagagaa aggtactgaa
     8101 gattcacggt gaccgacgac aatcacaccg tttttggcga ttttgtccag cgtactgcct
     8161 gctgccgggg cggcgtcatc tgcctgtgcc agtcctgcgg aaagcgccag ggcgaggatt
     8221 gctgtggcag gtttacgtaa ttgcatatcc aactccttta tcttctgcgt taagaacgca
     8281 tggatacccg ttgtgagtgt ttgtgttgtt atcgtctgca actttattgt gcagtgttgt
     8341 gcctgttagg taacatttag tttggctaaa tgtaaagata ttgctgtttt attgtttgtt
     8401 tttgcgagat gcgccgcacc attccgaagc aaaattctta aaccgcacct ttttggtgct
     8461 accgctgatt tgtggtggtg caacctggtt gcgtcgcggc tgatttcatc gcttacacag
     8521 taaataaagc aacttacatg ccaaaaaata cttaggggcg aattaattac gattgtaatt
     8581 ttaggctgaa aaagtcatat ttcggacaga attatctttt cggcatggga cgtttgccgg
     8641 gatgataact gcacacggga aaaatgcttt ttacctgtct tttcaaggaa gaaacacctt
     8701 atgtctgcat cttcggttaa gccgttaaat gttcaattac ccgcaataac ccttatcctt
     8761 tttgcactct gtattgggat attttgttat ctcgcacaat ggatgagtta tgaagaggtc
     8821 gatcaatccg cacttatcca tctcggcgca aacgttgcgc ctcttacttt gtcgggtgaa
     8881 ccctggcgct tattgagcag tatctttctg cacagtagtg tttctcatct gctgatgaat
     8941 atgtttgcat tcctggtcgt gggaggcgtg gcggaacaga ttctggggaa atggcgactc
     9001 ctgattacct ggttattctc cggcgtcttt ggtgggctca tcagcgcctg ttatgcgtta
     9061 cgcgaaagtg agcagatagt catcagcgtt ggggcatccg gggcgattct gggaatagct
     9121 ggtgcagcga tagcaacaca gtttgcttca ggtacgggca cataccataa aaaccagcgg
     9181 cgagtatttc ctctgttggg tatggtggcg ctgacactgt tgtacggtgc ccggcaaaca
     9241 ggaatagata acgcttgcca cattggcggc ctgattgcgg gtggcgcgtt gggttggctg
     9301 agcgcgcgtt tatctgggca aaaccgactc gttacggaag gcgggattat tgttgcgggc
     9361 agtcttcttc tgaccggggc tatctggctt gcgcagcagc agatggatga gtcagtttta
     9421 caggtcaggc aaagcctgcg tgaagcgttt tatccacagg agattgaaca agagcgacgg
     9481 caaaaaaagc agcagttagc ggaggaacgc aacgccctca gggaaacatt atccgctccg
     9541 gtaagtcgtg aacaggccag tggtgatttg ctcgctgaga ttgccgatat ccatgatatg
     9601 gcgatcagtc gggatggtaa tatgttgtat gccgcaattg aaaacacgaa cagcattgtt
     9661 gttttcgacc tcggacaaaa gaaaatcctg cataccttta cagcgcccat agcgaaagaa
     9721 aagtcagtca aacattgtgg tggctgtaaa gatcagggcg tcagatcgct ggcattaagc
     9781 ccggatgaaa agttgattta tgcgacttca tttgaagcga atgcgttatc ggtcattaac
     9841 gtggcgacag gggagattat tcagtcgatt accaccggtg cacatcctga cagccttatc
     9901 ctctcgcgtg atggcacaaa agcctgggtg atgaatcgca ccagtaatag tgtgtcagcg
     9961 attgatctgg tgacttatca gcatgtggcg gatatcccgc tggagaaata cgacgggacg
    10021 gggacgagcg gtaaaccagg cgcctgggtt atggcacttt ccccggatga aagaacatta
    10081 ctggttccgg gagcaggcag aggtaacatc gtgcggatca ataccatcac gcatcagaaa
    10141 gaagactttc ccgcaggtga tgcgcgtgga acgatatcgg cgatgcgttt tcgacctgaa
    10201 aacggcgagg ttatttttgc cgatagtcag gggatttcac gtataagcgt aggggatcaa
    10261 caagccagca ttatgacgca atggtgtagc aggagcgttt attccgttga gggtattagc
    10321 ccggacggtc aatatttagc gttggtgtca tatggcctgc aaggttatgt catcctgctc
    10381 aatattaatg ccgggcagat tattggcgtt tatcctgcca gctacgttaa tcaccttcgt
    10441 ttttcagcgg atggtagaaa aatatttgtc atggcgaaga acgggttgat ccaaatggac
    10501 aggacgcgct cgcttgatcc gcaggcaatt attcgtcatc cccaatatgg caatgtggct
    10561 tgtatccctg aaccgtaaga aaaaggggca tgataaccat gccccaaagc tcaatgagaa
    10621 agtacgtttt gctcatgcag gatacatcag gcacatcaac gtttacgtgc tcgcagactc
    10681 atcaacacag cggcaaaacc aaacagcgcc gtcagcaccc acagtggcca gttgccggta
    10741 cgtgcatacg gtgtgagtcc ggtggtcggt gtcacattag tggtcaacac ctcgcgggtg
    10801 aactgcggga tcatcgcctg aacctccccc tgcgggccaa tcaccgccgt aatgccgttg
    10861 ttggtgctgc gcaacagtgg gcgcgccagc tccagcgcac gcattcgcgc catctggaag
    10921 tgctgccatg gaccaataga cttaccaaac cacgcatcgt tggagatagt cagcagatag
    10981 tcggtatccg ggcggaagtt atcgcgcact tgctcgccga gaatgatctc gtagcaaata
    11041 gccgcagtaa gctcaatacc atttaccgac agcggcggct ggatatatgg cccacggctg
    11101 aacgacgaca tcggcagatc aaagaacggt gctaacggac gcagaatcga ctccagcggg
    11161 acaaactcgc caaacggcac tagatggttt ttgttatagc gatcggctga ttcgtagctg
    11221 tatggtgcac ctttacccag tgtgatgatg gtgttgtagg tatcgtagcg gttctgctta
    11281 ttgagacgcg cgtcgacaat cccggttacc agcgagctac ctttatcgcg caactcaccg
    11341 tccagtgctt tgaggaacgg ttgctgatta atttccagat cggttatcgc cgactccggc
    11401 cagataatca acgatgattt gcccatcagc ggagccgttg cgttgtagta aatcttcagc
    11461 gtattaagaa gctgatctcc atcccatttc agtgattgcg gaatatcgcc ctgaaccatc
    11521 gaaacctgaa tggttttctc cggttgcggg gtaaaccatt ggatgtaacg cagcgggaag
    11581 ggtagggcaa acagcacgac ggccaccacc agcggacgcc agttgcgttt gaccaacgcc
    11641 agtgccagca ggccactaac catcatcagc aggaagttaa tggcttccac gcccattatc
    11701 ggtgccagcc cttttaacgg gccatcaatc tggctatagc cgaactgtaa ccacgggaag
    11761 cctgtcagta cccaaccgcg cagaaactcg gtcacttgcc agagagcagg ggcggcaatc
    11821 gccacgcgca gccaggtggt tttcggccac agacgcgaca gcacgccagc aaacagtccg
    11881 gtatacagcg acaaatacgc cgccagcagc accaccagga agatgttaac cgggccaggc
    11941 attccgccaa aggtcgcgat gctgacatag acccagttaa taccgctgcc aaagaggcca
    12001 aatccccagc aaaagccaat agcggcagac tggagtggac ggcggttaaa ggtcaacgcc
    12061 tgaagcccca tcaacgaaat aatcgccgca ggccagacgt cgtaaggaga gaaggccagc
    12121 gttccgcagg caccgaataa taacgccagc agcaggcgaa tgcgctggcg ttcaattaat
    12181 gaggcaaaag ccatgtagtt atctatccag tttcggtttt attcatccag cttcggctgg
    12241 ggtgagtcat ccgggatttt gacatgaacc tgaataatac gccgactgtc ggccatcgcc
    12301 actttgaact ggtaaccgtc gatgtcgata gtttcgccac gcgccggaag atgcccaaat
    12361 gcctgcatca ccagaccacc gatagtgtca acctcttcat cgctaaagtg ggtgccgaac
    12421 gcttcgttga agtcttcaat ggaagccagt gcacgcacgg tccaggtatg acgactcagc
    12481 tgacggaagt cgatatcatc ttcttcgtca tactcgtctt caatctcacc aacaatcagt
    12541 tccaggatgt cttcaatggt caccagaccg gaaaccccac cgaattcgtc aatgacgatc
    12601 gccatgtggt aacgctgaga gcgaaactct ttcagcatcc ggtctacgcg cttactttca
    12661 ggaacgacaa ccgcctgacg taacactttg tccatgctga aggcttcagc atcgctgcgc
    12721 ataaacggca gcaagtcttt cgccatcaga atcccttcaa tgtgatcttt gtcttcgcta
    12781 atcaccggga aacgtgagtg ggcggactcg atgatgacat caaggcattc gtccagcgtc
    12841 tggttgcgtt tcagggtaat catctgggag cgggggatca tgatgtcgcg aacgcgttgg
    12901 tctgcgatgt ccatcacccc ttcgagcata tcgcgcgtat cttcgtcgat aaggtcgttc
    12961 tgcccggaat cacggatcag cgccagcagt tcgtcacggt ttttcggttc accgtggaaa
    13021 agttggctga gtaacaggga gaaaaatccc ttcttgttgc ttatcgtgtc actactgtgt
    13081 gaattgtcgt cgctcatggc gtcgtatggg ttctcatgtt agttaatcaa aacgccgtcg
    13141 ttaatcacca acggcgggga cgtctgccag tcaaatgcct ggcaaattat tctttctcgg
    13201 caatgtacgg atcctcatag cccagagcaa gcataatctc tgtttcgagg gcttccattt
    13261 cttctgcttc gtcatcttcg atgtgatcgt aacctaacaa atgcagactg ccgtgcacca
    13321 ccatatgcgc ccagtgcgcc tccagtggtt tgccttgctc ctgagcttcc ttctcaacca
    13381 cctgacggca gataaccaga tcgcccagta gcgacatttc catgccaggc ggcacttcaa
    13441 acgggaagga gagcacgttg gtcggcttat ccttaccgcg ataggtcaga ttcagactgt
    13501 ggctttcggc ggtatcgacc acgcgaatag tcacttccga ttcttcctga aactgcggga
    13561 tcaccgcatt cagccatgtt tgaaactggc tctcttccgg taacccggaa ttatcttcac
    13621 atgccagttg taaatcgagg atcacctgac tcatttttgt tcctgttctt cgcgcttgcg
    13681 ttctgccgcc agcgccgctt ttcgtttttg ttcggcttct tcccaggctt cataggcgtt
    13741 aacgatacgc gccaccacag ggtgacgaac cacgtcttcg ctgtggaaga agttaaagct
    13801 gatctcttcg acatcggcca gcacttcgat ggcgtgacgt aagcctgatt tagtattacg
    13861 cggcaggtcg atctgtgtga cgtcgccggt gataaccgct tttgagttaa aaccgatacg
    13921 ggtcaggaac atcttcatct gttcgatggt ggtgttctgg ctctcatcga gaatgataaa
    13981 cgcgtcgttc agcgtacgac cacgcatata ggccagcggc gcgacttcaa taacgttgcg
    14041 ctcaatcagt ttctcaactt tctcaaagcc cagcatttca aacagcgcgt cgtacagtgg
    14101 gcgcagatac gggtctactt tctggcttaa atcgccaggc aggaagccca gtttctcacc
    14161 ggcttcgacc gccggacgag tcagcagaat acggcgaatc tcctgacgct ccagggcatc
    14221 aactgccgca gccactgcca ggtaggtttt acccgtaccc gccgggccaa cgccgaaggt
    14281 aatgtcatgg tcgagaatat tggcgatgta ctgcgcctgg tttggcgtgc gcggcttaat
    14341 tacgccgcgt ttggttttga tattgaccgc tttgccgtac tccggcacgc tctccgcact
    14401 ttgctccagt actcgcgctt ctttaatcgc aaggtggatc tgttccggtt cgatatcctg
    14461 aatctgaccg cgcatcgggg ctgtatcgac atacaggcta cgcagaatgt ctgccgcagc
    14521 ggtgacgcaa atcggacggc cggtcagttt aaagtggttg tcgcggcgat tgatctcgat
    14581 gccgagacgg cgttcgagct gcttgatgtt gtcatcaaac gggccgcaca ggctcaacag
    14641 acgcgcgttg tctgctggct ccagggtgat ttcgcgagtg tctatgttca aaccgttcct
    14701 cttatctgta tgccgccgga agctgaacat tcaccggcct ataaggaaat tattcacgcc
    14761 acaggaaaaa ggcgcaagca ttgcgataaa gatggggata aagagagaaa aaacaaggcc
    14821 caccggaacg gcgggcctga gaattacggc tgataataac ccacgccaag gtcgttttct
    14881 ttgcgggtac gggcaatcac cgattccggt gtttctgcca cgcgcagacc catttcatct
    14941 tcagtacgca ccactttacc gcgcagagag ttcgggtaga cgtcggtaat ttctacatcg
    15001 acgaatttac cgatcatatc cggtgtgccc tcgaagttga ccacgcggtt attttcggta
    15061 cgcccggaaa gctccatgat gctcttacgc gaagtccctt ctaccagaat acgctgggtg
    15121 gtgccgagca tccggcggct ccatgccatc gcttgctgat tgatacgttc ttgcagaata
    15181 tacagacgtt gcttcttctc ttcttccgga acatcatcaa ccatatcggc ggctggtgta
    15241 cccggacgtg cagagaagat aaagctgtag ctcatgtcga aattgacgtc ggcaatcagc
    15301 ttcatcgttt tctcgaagtc ttcggtggtt tcgccaggga agccaacaat gaagtcagaa
    15361 ctgatctgaa tatccggacg cgccgcacgc agtttacgga tgatcgcttt gtactccagc
    15421 gccgtgtgag tacggcccat caggttcaga atgcgatcgg aaccgctttg taccggcaga
    15481 tgcaggaagc tcaccagctc cggcgtgtcg cgatacactt cgatgatatc gtcggtgaat
    15541 tcgatcggat ggctggtggt aaagcgaata cgatcgatcc cgtcgatcgc agcaaccaga
    15601 cgcagcagat cggcaaacga tccggtggag ccgtcgtagt tttcaccacg ccaggcgttc
    15661 acgttctgac cgagcaggtt gacttcacgc acgccctgag ccgcaagctg ggcaatctca
    15721 aacagaatat cgtcagacgg acggcttacc tcttcaccac gggtgtaagg caccacgcag
    15781 taggtgcaat atttattgca gccttccatg atggagacaa acgcggtcgg cccttcggcg
    15841 cgcggttccg gtagacggtc aaacttctcg atttccggga agctgatatc tacaaccggg
    15901 ctgcggtcgc cgcgcacgga gttgatcatc tccggcagac ggtgcagcgt ttgcggccca
    15961 aaaataatat cgacatagtg ggcgcgctgg cgaatgtgct cgccttcttg cgatgccacg
    16021 cagccaccga cgccgataat caggtctgga ttcttctctt ttaacagttt ccagcgaccc
    16081 aactgatgga agactttttc ctgagccttc tcgcggattg agcaggtgtt cagcagcagc
    16141 acatccgctt cttccgccac gtcggtcagt tgatagccgt gggtggcatc cagcagatcg
    16201 gccatcttcg atgaatcgta ctcgttcatc tgacagcccc aggttttaat atggagtttt
    16261 ttggtcatcg acttgctctt gcgaaatagt agccaggaat gcagggcgta tagtgtaatg
    16321 ctttgctgcc gttgtgacca gtatgagcgt tatcagccct tgggggtaaa aatcctgtaa
    16381 acttaaagca tattgctaac aggatgattg accatgacaa atcaaccaac ggaaattgcc
    16441 attgtcggcg gaggaatggt cggcggcgcg ctggcgctgg ggctggcaca gcacggattt
    16501 tcggtaacgg tgatcgagca cgcagaacct gcaccgtttg tcgctgatag ccaaccggac
    16561 gtgcggatct cggcgatcag cgccgcatcg gtatcattgc ttaaagggtt aggggtctgg
    16621 gatgcagtac aggctatgcg ttgccatcct taccgcagac tggaaacgtg ggagtgggaa
    16681 acggcgcatg tggtgtttga cgccgctgaa cttaagctac cgttgcttgg ctatatggtg
    16741 gaaaacaccg tcctgcaaca ggcgctgtgg caggcgctgg aagcgcatcc gaaagtaacg
    16801 ttacgtgtgc caggctcgct gattgcatta catcgagata atgatcttca ggagctggaa
    16861 ctgaagggcg gtgaaacgat ccgcgcgaaa ctggtgattg gtgccgacgg cgcaaattct
    16921 caggtgcggc agatggcggg aattggcgtt catgcctggc agtatgcgca gtcgtgcatg
    16981 ttgattagcg tacagtgtga gaacgatccc ggcgacagca cctggcagca atttaccccg
    17041 gacggaccgc gtgcgtttct gccgttgttt gataactggg catcgctggt gtggtatgac
    17101 tctccggcgc gtattcgcca gttgcagaat atgaatatgg cgcaattcca ggcggaaatc
    17161 gcgaagcatt tcccgtcgcg tctgggttac gttacaccgc ttgccgctgg tgcgtttccg
    17221 ctgacgcgcc gacatgcgtt gcagtatgtg cagccggggc ttgcgctggt gggcgatgcc
    17281 gcgcatacca tccatccgct ggcggggcag ggggtgaatc ttggttatcg tgatgtcgat
    17341 gccctgattg atgttctggt gaatgcccgc agctacggtg aagcgtgggc cagttatcct
    17401 gtcctcaagc gttaccagat gcggcgcatg gcggataact tcattatgca aagtggtatg
    17461 gatctgtttt atgccggatt cagtaataat ctgccgccgc tgcgttttgt gcgtaatctc
    17521 gggttaatgg cggcagagcg tgctggcgtg ttgaaacgtc aggcgctgaa atatgcgtta
    17581 gggttgtagc cttacaacat tgccggatgc gtgccaaccg taggtcggat aagacgcggc
    17641 agcgtcgcat ccgacattga aggataagac gtgtcaacat cgcattcgac attgaatgaa
    17701 cgcagaaaag caaaaaactc gccgaagcga gcttttttaa tgtggctggg gtacgaggat
    17761 tcgaacctcg gaatgccgga atcagaatcc ggtgccttac cgcttggcga taccccaaca
    17821 aattggtttt tgaatttgcc gaacgtattc gacacattca gaatttggtg gctacgacgg
    17881 gattcgaacc tgtgacccca tcattatgag tgatgtgctc taaccaactg agctacgtag
    17941 ccagattgtt tcttcgatgg ctggggtacc tggattcgaa ccagggaatg ccggtatcaa
    18001 aaaccggtgc cttaccgctt ggcgataccc caataaccgg tcggtgaacc gcttactcga
    18061 agaagatggc tggggtacct ggattcgaac cagggaatgc cggtatcaaa aaccggtgcc
    18121 ttaccgcttg gcgatacccc atccgtacaa cgctttctgg tgaatggtgc gggaggcgag
    18181 acttgaactc gcacaccttg cggcgccaga acctaaatct ggtgcgtcta ccaatttcgc
    18241 cactcccgca aaaaagatgg tggctacgac gggattcgaa cctgtgaccc catcattatg
    18301 agtgatgtgc tctaaccaac tgagctacgt agccatcttt ttttcgcgat accttatcgg
    18361 cgttgcgggg cgcattatgc gtatagagcc ttgcagcgtc aacctctttt tcaaggaaaa
    18421 ttgctcgaaa gtgactgttt ggttaggttg cgaacagcgt ggcgctatat tcgtcaatta
    18481 ttgtttactt tgtgtttttt ccccacccta cagccattct tttgtcatac aggatgaaat
    18541 tcggaattca acaatggtgg tgggaaatta atctatgaaa tactggccta cagtgatgag
    18601 ttgtcaaaca gtgatgtggc aaacccggaa catttcctta ctgcatatca gaatcaacaa
    18661 gcacctcaat aactgaaaca gccccggatt tcaccggggc tgtttcgcat ttcttactta
    18721 tacgccgact ggtgaacgcc aaccgcacga ccagacggat cgtccatttt cttgaacgcc
    18781 tcatcccact caatcgcttt agcggaagaa cacgcgacgg acgggccgcc cggcacgcac
    18841 tcagcggcgc tcggaagcgg gaacagttct tcaaagatct cccggtacag atacgcttct
    18901 tttgaggttg gcgtgttgta cgggaagcgg aagcgggcag tttccagttg ctgatcagaa
    18961 acctgctgcg ccgccacttc tttcagggtg tcgatccaac tgtaaccgac gccatcggag
    19021 aactgctctt tctgccgcca ggccacgctt gcgggcaggt atgactcaaa acattcacgc
    19081 aggatgtgtt tttccatttt gccgttaccg cacattttat cctgcgggtt aatgcgcatc
    19141 gccacatcaa ggaatttttt gtcgaggaac ggaacgcgtg cttccacgcc ccaggctgac
    19201 atcgctttgt tggcgcgcgc gcagtcatac atatgcaggg ccagcagttt acgcaccgtc
    19261 tcttcatgca gttctttagc gttgggcgct ttatggaagt aaagataacc gccaaacact
    19321 tcatcagaac cttcaccgga cagcaccatt ttaatgccca tcgccttgat cttacgcgac
    19381 attaaataca tcggtgttga agcgcgaata gtggtcacat cataagtttc gatgtggtaa
    19441 atcacgtcgc ggatggcatc cagaccttcc tgtacagtga agtgaatttc gtgatgcacc
    19501 gtgcccagat ggtttgccac ttcctgggct gctttaagat ccggtgaacc cggcagacct
    19561 acagcaaagg agtgtaactg gggccaccag gcttcagagc gttcctgatc ttccacgcga
    19621 cgggctgcgt atttcttggt gatagcggaa ataattgagg aatccagacc accagaaagc
    19681 agcacaccgt aaggcacatc agacatcaga tggcttttaa cggaatcttc cagagcctga
    19741 cgcagctcgt ttttgtcggt cacgttatct ttcactgcat caaagtcgaa ccagtcgcga
    19801 tgataataag aacggatttc gccgtcctgg ctccacaaat agctccccgc cgggaactct
    19861 ttaatcgtgc ggcaaactgg caccagcgat ttcatttctg aggccacata cagctgaccg
    19921 tgttcgtcat accccatata cagtgggatg atccccagat ggtcgcgacc aatcaggtag
    19981 gcatcttttt cgctgtcgta cagggcaaag gcaaacatgc cctgcaagtc gtcgagaaat
    20041 tccggccctt tttcctgata cagcgcgagg atcacttcac agtcagaccc tgtctggaac
    20101 tgataacgat cgccatattc agcgcgcaat gcctggtggt tgtagatttc accgtttacc
    20161 gccagtacgt gggttttttg ttggttgtag agaggttgcg cccccgcgtt aacgtcaaca
    20221 attgacagac gttcgtgagc gagaatggcg ttatcgctgg cataaatacc ggaccagtcc
    20281 gggccacgat gacgcatcag gcgtgacagc tcgagtgctt tcttacgctg ctcaactgcg
    20341 tctgttttga tatcgaatac gccaaaaatt gaacacataa ccttctccgt taacctggta
    20401 tttgttgctt gttgtgtttg cttgtttaaa aaaatgccgc aaagcagcac tgtgcgcaag
    20461 cgatttggcg gtgaaaaaat aaaaaacgta atggtgattg tcgattggtg aaaaaggggc
    20521 tggtgtgagg atgtatttat tgatgaatcg ataattttta gcgggtttta ttgaatggtt
    20581 atattttacg ggg
//
LOCUS       DAZPJP010000059        20427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPJP010000059 DAZPJP010000000
VERSION     DAZPJP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.059"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHU9J_001991"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHU9J_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1905536.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHU9J_001992"
     CDS             433..621
                     /locus_tag="ACHU9J_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1905537.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHU9J_001993"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHU9J_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1905538.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHU9J_001994"
     CDS             816..989
                     /locus_tag="ACHU9J_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1905539.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHU9J_001995"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHU9J_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHU9J_001996"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHU9J_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1905540.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHU9J_001997"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHU9J_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1905541.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHU9J_001998"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHU9J_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1905542.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHU9J_001999"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHU9J_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1905543.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHU9J_002000"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHU9J_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHU9J_002001"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHU9J_002001"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1905544.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHU9J_002002"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHU9J_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1905545.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHU9J_002003"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHU9J_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1905546.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="ACHU9J_002004"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="ACHU9J_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="ACHU9J_002005"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="ACHU9J_002005"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1905547.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="ACHU9J_002006"
     CDS             complement(15250..15477)
                     /locus_tag="ACHU9J_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1905548.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHU9J_002007"
     CDS             complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHU9J_002007"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1905549.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16467..16640
                     /locus_tag="ACHU9J_002008"
     CDS             16467..16640
                     /locus_tag="ACHU9J_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1905550.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHU9J_002009"
     CDS             complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHU9J_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1905551.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHU9J_002010"
     CDS             complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHU9J_002010"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1905552.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18752..19342)
                     /locus_tag="ACHU9J_002011"
     CDS             complement(18752..19342)
                     /locus_tag="ACHU9J_002011"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1905553.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHU9J_002012"
     CDS             complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHU9J_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1905554.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHU9J_002013"
     CDS             complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHU9J_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1905555.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         5106 a         5200 c         5243 g         4878 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tatatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc
    16081 agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac
    16141 cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat
    16201 ttataacaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg
    16261 cgttttttta atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc
    16321 taagaagctg ttgcaggata ttaccaaacg cgggtctacc cgcgtcagtt cactaagctt
    16381 agtcccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga
    16441 tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg
    16501 cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta
    16561 atttcaagaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc
    16621 acggcaaaag cgacaactag ccgggctaat caatgacgaa tgcatttttg tctgtagctc
    16681 gtcaaaaagc catcaccgcc ggttacccgg tggttgatac tgatgacaaa tgtaagcttg
    16741 cctgatgcgc gatgcttatc aggcctacca gaagattgca atatattgaa tttgcactgt
    16801 tttgtaggcc ggataaggcg tttacgccgc atccggcatg aacaatgcgt acgttgtcaa
    16861 caatctgcac cgccgggtaa ccccggcggt tttctgttta tggctcctga tgaacaactt
    16921 ctggcggtgg aacgtcaacc aaccttctac ttaacaacgc attgagtaaa atcgcgccaa
    16981 aggtggctgt accgatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    17041 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    17101 ggttttgtac ccagatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    17161 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
    17221 ccaacagcat ggcgatgacg gcagcagcaa caaacaccag cgtcgagtag acttttgtca
    17281 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
    17341 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
    17401 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    17461 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    17521 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    17581 cgttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    17641 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    17701 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    17761 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    17821 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    17881 tcccgcccag tgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    17941 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
    18001 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    18061 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    18121 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
    18181 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
    18241 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    18301 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    18361 gacgctcacc acctggctga tgcggcagtc aaacgaaacc agagcctctt ccagttgcgg
    18421 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    18481 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    18541 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    18601 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggatgg
    18661 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
    18721 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
    18781 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    18841 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    18901 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct
    18961 gcatggagct gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    19021 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
    19081 caacgatagc ggtgacgggc gcggtcaggg ttttttgtag attgccgctg gaaagcgccg
    19141 ggtgcagacg ttcttttcct tctacagtgc ggataaacac gatccgtgcc ggagaacagt
    19201 tagctgatgt cggcccccat tttatcgggg cataaagctc ccgtaacgtc tcatcgctga
    19261 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
    19321 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    19381 ggctggcaag cccgttgagt aacagagcat taaacgtttc ggggtcggtc acgttgcagg
    19441 cgtgtccgcc atagcgcatc accattttct ggctatcggg cagggcggca tgaagctcac
    19501 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    19561 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    19621 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctca aggcaaggtg
    19681 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    19741 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    19801 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    19861 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    19921 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    19981 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    20041 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    20101 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    20161 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    20221 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    20281 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    20341 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
    20401 tttgggtcat cggcaaacag cacgtta
//
LOCUS       DAZPJP010000060        20421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPJP010000060 DAZPJP010000000
VERSION     DAZPJP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.060"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(50..475)
                     /gene="nudI"
                     /locus_tag="ACHU9J_002014"
     CDS             complement(50..475)
                     /gene="nudI"
                     /locus_tag="ACHU9J_002014"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1905556.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            754..1296
                     /locus_tag="ACHU9J_002015"
     CDS             754..1296
                     /locus_tag="ACHU9J_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1905557.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1396..2598
                     /locus_tag="ACHU9J_002016"
     CDS             1396..2598
                     /locus_tag="ACHU9J_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1905558.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2818..3600
                     /locus_tag="ACHU9J_002017"
     CDS             2818..3600
                     /locus_tag="ACHU9J_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1905559.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3615..4820
                     /gene="rhmD"
                     /locus_tag="ACHU9J_002018"
     CDS             3615..4820
                     /gene="rhmD"
                     /locus_tag="ACHU9J_002018"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR1905560.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4877..6166
                     /locus_tag="ACHU9J_002019"
     CDS             4877..6166
                     /locus_tag="ACHU9J_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905561.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6184..6987
                     /gene="yfaU"
                     /locus_tag="ACHU9J_002020"
     CDS             6184..6987
                     /gene="yfaU"
                     /locus_tag="ACHU9J_002020"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1905562.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7028..7930)
                     /locus_tag="ACHU9J_002021"
     CDS             complement(7028..7930)
                     /locus_tag="ACHU9J_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1905563.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8123..9313)
                     /gene="glpC"
                     /locus_tag="ACHU9J_002022"
     CDS             complement(8123..9313)
                     /gene="glpC"
                     /locus_tag="ACHU9J_002022"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1905564.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9310..10569)
                     /gene="glpB"
                     /locus_tag="ACHU9J_002023"
     CDS             complement(9310..10569)
                     /gene="glpB"
                     /locus_tag="ACHU9J_002023"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1905565.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10559..12187)
                     /gene="glpA"
                     /locus_tag="ACHU9J_002024"
     CDS             complement(10559..12187)
                     /gene="glpA"
                     /locus_tag="ACHU9J_002024"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1905566.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12460..13818
                     /gene="glpT"
                     /locus_tag="ACHU9J_002025"
     CDS             12460..13818
                     /gene="glpT"
                     /locus_tag="ACHU9J_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1905567.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13823..14899
                     /gene="glpQ"
                     /locus_tag="ACHU9J_002026"
     CDS             13823..14899
                     /gene="glpQ"
                     /locus_tag="ACHU9J_002026"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1905568.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14941..15183)
                     /locus_tag="ACHU9J_002027"
                     /pseudo
     CDS             complement(14941..15183)
                     /locus_tag="ACHU9J_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15178..15282
                     /locus_tag="ACHU9J_002028"
                     /pseudo
     CDS             15178..15282
                     /locus_tag="ACHU9J_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15362..16011
                     /gene="inaA"
                     /locus_tag="ACHU9J_002029"
                     /pseudo
     CDS             15362..16011
                     /gene="inaA"
                     /locus_tag="ACHU9J_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16065..16319)
                     /gene="yfaE"
                     /locus_tag="ACHU9J_002030"
     CDS             complement(16065..16319)
                     /gene="yfaE"
                     /locus_tag="ACHU9J_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1905569.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16319..17449)
                     /gene="nrdB"
                     /locus_tag="ACHU9J_002031"
     CDS             complement(16319..17449)
                     /gene="nrdB"
                     /locus_tag="ACHU9J_002031"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1905570.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17683..19968)
                     /gene="nrdA"
                     /locus_tag="ACHU9J_002032"
     CDS             complement(17683..19968)
                     /gene="nrdA"
                     /locus_tag="ACHU9J_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1905571.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            20096..20239
                     /gene="ypaB"
                     /locus_tag="ACHU9J_002033"
     CDS             20096..20239
                     /gene="ypaB"
                     /locus_tag="ACHU9J_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1905572.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4756 a         5002 c         5526 g         5137 t
ORIGIN      
        1 ttaaccacta aattttcaat ctgacagcca gtgatggctg tcatcattgt taaagaagac
       61 ctttcaaacg taacgttttt cgggtggcaa catttaaatc ataatgcacc agatcttcag
      121 gttttaccca cgcgtagtcc tgaaactctt cgtttatttt cacttctcgg ttggcagaaa
      181 cgcagtcaaa aatcaggtaa atcatataaa tctcttcctt gcgaccatct gcatacgtct
      241 tggtgcgaat atcatcgctg aaggtccacg gcgtgatttc tgtcaaaagc agctgttctc
      301 ccagttcttc gcgaatttcg cggcgtagtg cctcttcaat tcgttcgcca gactccacgc
      361 cgccacccga aagcgcccat tgaccgggga aaacgccgcg atcgtcggcc attttgcaca
      421 gcaaataagc accatcattt tgaatcaaag ggcatacaat agtccgttgt cgcacagggg
      481 tattccttat aaataaaaag aaaccatgag aatacggtaa ctcagaaata tgtgaaagtg
      541 acagtaatct cttttgcgat gataactgca cttattatta tttttgtgtg tatcggtaaa
      601 gtgtgtttat tttttcggcg tttaattatt taagaagtat ttagttacaa taagtgacta
      661 gatccctttt ttcgctcatt gaatcctctg tgccaggcag tacaatcccg cagctttggg
      721 aattttgatt aattgcttgt gggaaagggg ataatgaaaa aaattgcgct agcaggtctg
      781 gccggaatgc tgttggtttc tgcatcggtc aatgcaatga gcatcagcgg tcaggcgggt
      841 aaagaataca ccaatattgg tgtcggtttt gggactgaat cgacgggcct ggcgttaagc
      901 ggtaactgga cacataacga cgacgacggt gacgtcgctg gcgtggggct ggggttgaat
      961 ctgcctctcg ggccgttaat ggcgaccgtt ggcggaaaag gcgtttacac caacccgaat
     1021 tacggcgatg aaggttatgc cgcagcggta ggaggtggtt tgcagtggaa aattggcaac
     1081 agcttccgtt tgtttggcga gtattactac tctccggatt cactctccag cggtattaaa
     1141 agttatgagg aagcgaatgc tggcgcgcgt tacaccatta tgcatccagt cagtattgag
     1201 gcgggttatc gctacctgaa tctgtcgggt aaagacggta accgcgacaa cgccgtggct
     1261 gacggcccgt atgttggggt taacgccagt ttctgatccc ttccggcgcg gcattctgtc
     1321 gcgccattca gtttcttact acgccttatc tccctttgcg ttatagtgat tcgataacaa
     1381 aagcaggaga taaccatgtt aaaagtggaa atgttatcca ccggggatga agtgttacac
     1441 gggcaaatcg ttgacactaa cgctgcctgg ctggccgatt ttttctttca tcaggggttg
     1501 ccattatctc gccgcaatac ggtgggggat aaccttgatg acttagtcac cattcttcgc
     1561 gaacgtagtc agcacgccga tgtgctgatc gttaacggcg ggctgggacc gaccagtgat
     1621 gatttaagtg cactcgccgc tgcgacagca aaaggtgaag gcctggtgct gcatgaagcc
     1681 tggctcaaag agatggaacg ctatttccac gaacgtggac gagtaatggc accgagcaac
     1741 cgtaaacaag cggagctgcc tgccagtgct gaatttatca ataacccggt aggcaccgcc
     1801 tgtggttttg ccgtgcggct taatcgttgc ctgatgttct ttactcccgg cgtaccgtca
     1861 gaatttaagg tgatggtcga gcacgaaatc ctgccgcgcc tgcgcgagcg tttttcttta
     1921 ccacagtcgc cggtttgtct gcgtttaact acctttggtc gttcggaaag cgatctggca
     1981 caaagcctgg acactctaca actgccgccg ggtgtaacaa tgggctatcg ctcctcaatg
     2041 ccgatcatcg aactgaaact caccggaccg gcaagcgagc aacaggcgat ggaaaaactg
     2101 tggctggacg ttaaacgtgt tgccggacag agcgtgattt tcgaaggcac cgaaggactg
     2161 cccgcgcaga tcagtcgcga attgcaaaac cgccagttca gcctgacgtt gagcgagcaa
     2221 ttcaccggtg gtttattggc tttgcaactt tctcgcgcag gtgctccatt gctggcgtgt
     2281 gaagtggttc cttcacagga agaaaccctg gcgcaaactg cgcactggat tacagaacgg
     2341 cgggccaacc attttgccgg gctggcactg gctgtttcgg gtttcgagaa cgagcatctc
     2401 aactttgcgc tagccacgcc agacggcact ttcgctctgc gtgtgcgttt cagcactaca
     2461 cgctacagcc tggctatccg tcaggaagtg tgcgcaatga tggcactgaa tatgctgcgc
     2521 cgttggttaa acggccagga tatcgccagt gagcatggct ggattgaggt tgttgagtcc
     2581 atgaccttat ctgtctgatt ccgccactct gtgccggttt actgcaccgg cacattcccc
     2641 ctccggcaaa acttgtccaa cctgttgtac tcaataagaa aaaatccgtg cactgattca
     2701 caaatttgtg cgtaatttct ccagtccgct ggacgaggag cgggatttga tgcgcttcac
     2761 aattttactc cccctgagca acgagactga ttccagtatc taagactgga gtccagtatg
     2821 ttggaatcaa gcaaagttcc cgccctgaca cgggctattg atattctcaa tttgattgcc
     2881 cgcattggcc cctgtagtgc tgcgacgatc attgacacat tgggaatccc taaaagcacg
     2941 gcctatttgc tgcttaatga actcaggcgt cagcgttttc ttagccttga tcaccaggaa
     3001 aatttttgtt tgtggaccag gctggtggag ctttctggtc atgcgttaag caagatggat
     3061 ctccgggaac tggctcgacc gcgtttgacg caactgatgg ataccaccgg gctgttatgt
     3121 cacctgggga ttattgataa cggcagcgct tattacattt taaaagtgga gtcatcagcc
     3181 actatcagcg tgcgttccca tgaaggaaaa agcctttcgc tttatcgctc cggaataggt
     3241 aaatgcctgc ttgcctggca acctgcagcg gtacagcaaa gtattatcga agggctggtc
     3301 tgggagcagg cgacacccac aacgattact catccacagc aacttcacga agaactggcg
     3361 cgtattcgcc gtcagggctg gagctatgac aacggcgaag attaccccga tgtccgttgt
     3421 gtcgctgcgc cggtgtttaa cgccaataac gagcttaccg ctgcgatctc ggtggtcggt
     3481 acccgcttac aaatcaatga agaataccgt gattatctgg cgggcaaggc cattgcctgc
     3541 gccagggata tttcacgtct gttgggatgg aaaagtccct ttgacttaca ggcctcataa
     3601 caatggagaa catcatgacc ctaccaaaaa tcaaacaggt tcgcgcctgg tttactggcg
     3661 gtgcgacagc agaaaaaggt gctggcggcg gtgattatca cgaccagggg gcgaatcact
     3721 ggattgacga tcatattgcc accccgatga gtaaataccg cgattacgag cagtcacgcc
     3781 agtcatttgg cattaacgtt cttggcacgt tgattgttga agtcgaagca gaaaacggcc
     3841 agaccggatt cgctgtttcg acagccggtg aaatgggctg ttttattgtc gaaaaacatc
     3901 ttaaccgttt cattgagggt aaatgtgtca gtgatatcaa actgatccat gatcaaatgc
     3961 tcaatgccac cctgtattac tccggctctg gtggcctggt gatgaacacg atttcctgtg
     4021 tcgatctggc tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt
     4081 taggcggcgc agttcgtgat gagattcagt tctacgccac aggcgcgcgt ccggatctgg
     4141 caaaagagat gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg
     4201 atgcggggat ccgcaaagat gccgttatgg tcgcggatat gcgtgaaaaa tgcggtgagg
     4261 atttctggtt aatgctcgac tgctggatga gtcaggacgt gaactatgcc accaaactgg
     4321 cccacgcttg cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt
     4381 atgaaggtta tcgcgaactg aaacgcaacg cgccagtcgg aatgatggtc accagcggtg
     4441 agcaccacgg cacactgcaa tcttttcgta cgctttcaga aaccggtatc gacattatgc
     4501 agccggatgt tggctggtgc ggtggtttaa ccacgctggt ggaaattgcc gcaatcgcca
     4561 aatccagggg gcaactggtg gtgccgcacg gttcgtctgt ttactctcac catgcggtga
     4621 tcaccttcac caatacgcca ttcagcgaat tcctgatgac cagcccggat tgttcaacga
     4681 tgcgtccgca gtttgacccg attctgctca atgagccggt tccggtgaat ggtcgtattc
     4741 ataaatcagt gcttgataaa cccggttttg gcgtcgaact caatcgtgac tgcaatttga
     4801 aacgccccta cagccactaa tcactccggg cgttgctgtg acaacggtaa cgcccacata
     4861 ttctaaggag ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgt
     4921 gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc gttcgaatat
     4981 cggttttgcc aaacagacct accagattga taccgggttg agtaatgaag cttatgcgct
     5041 gggagcaggc attttctttg tggtatatgc gtttctgggt gttccggcga atcttttgat
     5101 gcgcaaactg ggggccagaa cctggattgg tacgacaaca ctgctgtggg gatttctttc
     5161 ggcagccatg gcatgggccg atactgaagc gaaatttctg atagttcgca ctctgcttgg
     5221 tgctgcggag gccggatttt tccctggtat gatttatctc acctcgcaat ggtttccgca
     5281 gcgtaatcgc gccagcatta tggggctgtt ctatatgggc gcaccgctgg cgttaacact
     5341 gggatcaccg ctttccggcg cgctgttgga gatgcatgga tttatggggc atcccggctg
     5401 gttctggatg tttgtgattg aaggattgtt ggcagtcggt gctggggtat tcacattctt
     5461 ttggcttgat gacacaccgg agcaggcacg ttttctgagt aaacaagaaa aaacgttgct
     5521 tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact tctcggctga gcgatgcgct
     5581 gcgtaatggc cgagtctggc aactagcgat tatctacctg accattcagg tggcggttta
     5641 cggattaatt ttcttcctgc cgacccaggt tgcggcattg ctgggaacaa aagtgggctt
     5701 tacagcgtcg gtggtcaccg ccattccgtg ggttgcggcc ttgtttggga cctggcttat
     5761 tccgcgctat tccgataaaa ccggcgaacg gcgtaatgtc gcagcgctga cattactggc
     5821 ggcgggcatt ggtattggtc tgtccgggct gctttctcca gtaatggcga tcgtagcgct
     5881 gtgtgttgca gctatcgggt ttattgccgt gcagccggtg ttctggacga tgccgacaca
     5941 actgctttcc ggtacggcgc tggctgcggg gattggtttt gtaaacctgt ttggtgcagt
     6001 gggcgggttt attgccccga tcctgcgcgt gaaagcagaa acgttatttg ccagcgatgc
     6061 ggcaggatta ctgacactgg cagcggtggc ggtcatcggt tcgctgatta ttttcactct
     6121 gcgtgtaaat cgtactgttg cgcagaccga cgtggcacat cattaaatag gttaaggaac
     6181 acgatgaacg cattattaac caatcccttt aaagaacgtt tacgcaaggg cgaagtgcaa
     6241 attggtctgt ggttaagctc gactacttct tatatggcag aaattgccgc tacttccggt
     6301 tatgactggt tgctgattga cggggagcac gcgccaaaca ccattcagga tctttatcac
     6361 cagctacagg cggtagcgcc ctatgccagc caacccgtga tccgtccggt ggaaggcagt
     6421 aaatcgctga ttaaacaagt tctggatatt ggcgcgcaaa ctctattgat cccgatggtc
     6481 gatactgccg atcaggctcg tcaggtggcg tctgccacgc gctatcctcc ctacggtgag
     6541 cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga gaattacatg
     6601 gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac ggcactggat
     6661 aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg accagcggat
     6721 ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca gcgaattatt
     6781 gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct
     6841 cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac
     6901 acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc aggcaaaaat
     6961 gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg ctgtgggcct
     7021 gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg gccgcgagca
     7081 cctggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg gcttgttcat
     7141 gcataccttc ctgccagcca atttggtgtc cttcctgccg tagccgctca gcaatagtca
     7201 tcaatctctc cttttgtagt ggtgaacgtt cggaaatctc ctcaataaaa cgggtgaaac
     7261 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     7321 tgaagccttt aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     7381 gaatatgctt ttgaatcagt tcaagcagag ctatacgccg atgttgcatg atctcatcgt
     7441 caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     7501 gcggatcgtc aaactcatcc agccagttaa gtgagtacgg gtagggcgat gtttcgccat
     7561 gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc aggtgacgct
     7621 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     7681 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     7741 catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc aggcgtaagg
     7801 tttgcaggtt gcaaagcttg cgcagtggtt ctggcaaatg tatttcgaga aaatcccgtg
     7861 cggtttcggg tgtgaacata aaggttttaa ataccgcatc atgcggggag gaggttgttg
     7921 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     7981 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     8041 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     8101 ttaccgggca tcagaaagga gtttaagcca gcgcctgggc cagtagcgta atcggatgtt
     8161 cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     8221 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     8281 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     8341 gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc
     8401 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     8461 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat
     8521 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     8581 tacaggttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     8641 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg
     8701 gtacgccgca gcatttttct ttgctgagca gttgtacacc gatacccatt gcgttgagca
     8761 ctttaattaa atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     8821 aagcgacctg gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgga
     8881 acgtgccgaa ggagtatttc ggtagcgtgc gacgatgatc gatttttaac gccgcatcaa
     8941 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
     9001 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
     9061 tgtcatattt tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg
     9121 cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat
     9181 ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat
     9241 tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
     9301 tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
     9361 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
     9421 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctc
     9481 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca
     9541 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt
     9601 acgttccgct accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg
     9661 acgtagcgga atatcggcgt gattacgggt ccagatttca ttcactacgc catttttaca
     9721 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg
     9781 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
     9841 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa
     9901 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
     9961 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt
    10021 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag
    10081 atccagttcg cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat
    10141 ccccactaca catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa
    10201 ccaggttgca cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc
    10261 ctgcaattgc gcaccgcttt cagcgatcaa tgcctgcgcc tggcaagcga gatcgagcac
    10321 gcgttgtggt tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag
    10381 tccactgtga atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga
    10441 accggatgag aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg
    10501 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
    10561 aaagcgcatc tttctgctcc ttctccagac cacacagtcc ctgataaacc cagcgggtaa
    10621 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    10681 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca
    10741 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg
    10801 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    10861 cacccgctgt taccgcttcg cactcacata ccaggctacg gtgcagacga ccttcgctca
    10921 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
    10981 gggagatgac tttacgcaag gtaacttcag cgggttcctg tgaaccagga agtgccagat
    11041 cggcggtcgt acaggggcgc gtgttgccca gtttgcggca cacggcgtcg gtagcccatt
    11101 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
    11161 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
    11221 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
    11281 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
    11341 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa
    11401 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat
    11461 gctggttaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
    11521 gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta accacgacag
    11581 gcgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg
    11641 tcgcgccttc acgaatcagt cccgtgactt catgagcggt aaggataacg gcaccgtgtt
    11701 ctttggcatc cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa
    11761 ctttcaccgc gccaatcaat gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
    11821 ggtctatagc ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga
    11881 aggagaggtc atcttccggc agggtaataa acaggccgtt ggttggttca acgcagtgac
    11941 gtgcaatgcg tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg
    12001 ttaccgcata gcgcgcaccg ctgtgtagca ggccgtggtt acgcccggtg gcaccggttg
    12061 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
    12121 tcccggctcc cgttgcgccg ccgccaatga taatcacgtc acttgattgc gagtcgcgag
    12181 ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa
    12241 ttgtttgatt tcgcgcatat tcgctcatga ttcgaaagtg aaacgtgatt tcatgcgtca
    12301 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
    12361 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctctcact gaatcaggct
    12421 gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
    12481 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
    12541 tggcaaattt tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac
    12601 tttgcgcttg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
    12661 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
    12721 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
    12781 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg
    12841 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
    12901 tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc
    12961 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat
    13021 gcggcgctct atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg
    13081 atgcgcgata ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat
    13141 ccggacgact ataacgaaaa agcggaacag gagctgacgg cgaagcaaat cttcatgcag
    13201 tacgtactgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg
    13261 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    13321 ctggataaat cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcactctg
    13381 ctgtgcggct ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc
    13441 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    13501 accgtcgata tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg
    13561 atcggtctgc atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt
    13621 accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    13681 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg
    13741 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa
    13801 aaacgcaacg gaggctaatg gcatgaaatt gaagctgaaa aaccttagca tggcgatcat
    13861 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    13921 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    13981 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    14041 tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc
    14101 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    14161 gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtac agacttatcc
    14221 ggggcgtttc ccaatgggta agtccgactt ccgggggcac acctttgaag aagagattga
    14281 atttgttcag gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaatcaa
    14341 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct
    14401 gaagaaatat ggttacaccg gtaaggacga taaagtttat ttgcaatgtt ttgatgctga
    14461 tgagctgaag cgtattaaga atgagctgga acctaaaatg ggcatggatc tcaatctggt
    14521 acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg
    14581 ggttaattac agctatgact ggatgtttaa gccaggtgcc atgaagcaag tcgcggaata
    14641 tgccgacggt atcgggccgg attaccatat gttgattgag gaaacatcgc agccgggtaa
    14701 tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta
    14761 taccgtgcgg tcggataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc
    14821 tctgtataac aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa
    14881 attccttaat aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcata
    14941 tcaaattttt ccaatcgcca ctaattgttc catgcacatt tgtagtaaaa acagtggact
    15001 gccttctacc ggcttttctt tagccaacaa cgaaatctgt cgatagaagg ttggttcgtg
    15061 aggaacggaa cacaattcac ctgcaatggt tttcagcgtt aactctggta ataaggcgat
    15121 gcctaatccc tgtcgaataa aattcatcgc tgtatccacc tgattaaatt cacattttat
    15181 ctgctgggcg cactattagt ggtcgtggct ggtggaatct atgcgctgca aatgaagggc
    15241 aaaagcggtg tcacactagt agcggcaaaa gaaattcatt aatacgacac gtttcattaa
    15301 gattttcctc aggtaaccac ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg
    15361 tatggcagtt tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg
    15421 ggttgaagag cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa
    15481 cggcaaaaag ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc
    15541 gttcggccga ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg
    15601 tgtcatcgtg ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgcgc
    15661 gttgctggtg actgaagata tggcggggtt catcagcatt gctgactggt atgcccggca
    15721 tgcagtatcg ccctattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc
    15781 gtttaagaaa atgcatagcg ttaatcgtca gcatggctgt tgttatgttc gccatatcta
    15841 tgtgaaaaca gaaggtaaag cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg
    15901 cttgcgtcgc aataaagcga ttaatcatga cttccgccag ctggaaaata tctggagcca
    15961 atccccaaag cggactggga gcaggtgaaa gcgtattact acgccatgta atcgttgtag
    16021 gccagataag acgtataagc gttgtatctg gcactacaac caattcacat ctcgatttca
    16081 atatcgcctt ttgcccggca acaacagggc aaaatttccc ccggctgaat aaaggctaac
    16141 ggttcggcaa tccagtccac ctgacctgcg accagtcggg tgcgacagga gccgcagtaa
    16201 ccttcgcggc actggtactc aaccgcaaca ttgtgggatt ccagcgccgc cagaagggaa
    16261 gggtgttcat cctggcacag cagttgtgtg ccagtgatgc gcagggtaac gcgggccatc
    16321 agagctggaa gttactcaaa tcgtcggtgt ccacttccgc gtcaatctgc ccgaccagat
    16381 aagaactgac ttccacttcc tgcggagcaa cctgcacgtt atcagacacc agccaggtgt
    16441 tgatccacgg gatcgggttg gagcgcgtct ggaacggcag atccaaaccg actgcctgca
    16501 tgcggatatt ggtgatgtat tcaacgtact ggcagagaat atctttattc agaccaatca
    16561 tcgaaccgtc acggaacaga taatccgccc agtctttctc ttgctgtgcc gcctggacaa
    16621 acaggtcata gcactcctgc ttacattctt cggcaatttc cgccatctcc ggatcatccg
    16681 cgccgctgcg cagcagattc agcatatgct gagtaccggt caggtgcagg gcttcatcgc
    16741 gggcaatcag gcgaataatc ttggcgttac cttccatcag ttcgcgttct gcaaaggcaa
    16801 aggaacaagc aaagctgacg tagaagcgga tcgcttccag cgcgttaacg ctcatcaggc
    16861 agagatacag ttttttcttc agctcgcgca ggctaacggt tacggtttta ccgttaacgg
    16921 tgtgggtgcc ttcgcccaga aggtgccagt agctggtcat ttcgatcagt tcatcgtaat
    16981 agctggagat cccttccgca cgtttctgga tctgctcgtt ggtgacgata tcgtcaaaca
    17041 caacagacgg atcgttaacg atattacgaa tgatatgagt ataggaacgg gaatgaatcg
    17101 tttctgagaa cgcccaggtt tcgacccagg tttccagttc cggaatagaa ataagcggca
    17161 atagcgccac gttcgggcta cgaccctgaa tggaatccag cagcgtctga tatttcaggt
    17221 tgctgataaa gatgtgtttt tcgtgctccg gcagcgcctg gtaatctata cggtcgcggg
    17281 agacgtcaac ttcttccgga cgccagaaga aagagagctg cttttcgatc agcttttcga
    17341 agatgtcata tttttgctga tcgtagcgag ccacgttgac cggctgacca aagaacatcg
    17401 gttctttgag ctgatcattt ttcgtctgtg aaaaggtggt atatgccatg agtgtgtcct
    17461 gttgggagtt taatgccgga taaggcattt tacgccgcat cctgtgcctg atgcgacgct
    17521 ggcgcgtctt atcaggccta caaatccgag ccgtaggccg gataaggcgt tcacgctgca
    17581 tccggcaccc ggagcctgat gcgacgctgg cgcgtcttat caggcctaca aaccgagccg
    17641 taggccggat aaggcgttta cgccgcatcc ggcatctcaa tatcagatct tacatgcgcc
    17701 gctttcgcag ccatcgtcct ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc
    17761 gtcacgggtg ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc
    17821 tttcagcaac tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt
    17881 gttagcagag atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc
    17941 atcgttaccc ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg
    18001 caccacctgg cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg
    18061 ttcaataccg ttagtggcgt tagagatctg cgaagaggtc tcggacggca tcagggcaga
    18121 aagcgtagag ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta
    18181 atgcagcggc tcattagcga tggtatccag atctttctta taggtatcga tcggcaggat
    18241 ccctttcgcg taagtggttt cgttaaacca cgggcacgcg ccttgctctt tcgccagctc
    18301 attagaggct ttcagcaggt aatactgaat ggcttcgaag gttttatgcg tcaggttgtt
    18361 ggcgctgccg tcggagtagc gtttaccgtg cttcgccagg tagtaagcga agttgatcac
    18421 accaataccc agcgtacgac gacccatcgc tccacgtttg gcggccggga tcgggtaatc
    18481 ctgataatcc agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc
    18541 atccaggtta ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc
    18601 gttctcgtcg ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt
    18661 agactgacgc actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac
    18721 gttctgaata tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc
    18781 aacggctttc acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacagacg
    18841 ttcaaactct tcctgatcgg cgaagaacgc gtcgtacagc cccggtacgt cggacgggct
    18901 gaacagggtg atatcttcac ctttcagcag acgggtatac atcagtttgt tgatttgtac
    18961 cccgtagtcc atatgacgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag
    19021 caggctttcc acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac
    19081 accgccctga gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc
    19141 ggtatggaac gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt
    19201 gatgccgatc ccggcacgct gggaaacgta tttaacaatc gcgctggagg tggcgttgat
    19261 ggaatccagg ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt
    19321 acgcacgccg gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta
    19381 aaaacgcttc acatattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc
    19441 aaccagaata taaaggaact gggcgctctc atagatttcg ccggtcacgc ggttctgtac
    19501 cagatatttg ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg
    19561 atcgataaac gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt
    19621 atcgtatttg cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa
    19681 ctggccgtag gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata
    19741 atccggcgca tcacgggaga tcaggtctgc ggcagccttg ataatggttt cgtggatgtc
    19801 agaggtcttg ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga
    19861 aacgttatgc agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat
    19921 gcgctctgtg ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc
    19981 tgtttttgga attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc
    20041 ttagaacact atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatgac
    20101 cctcttgcaa gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac
    20161 cttaggtcag acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa
    20221 ctgttcggcg atttcttgaa cggcagaata ggttggataa attgatatag atggtttatt
    20281 aatctttttg ttaatgattg caaaaattga tctaagcacg agtcatcaca taatctgatg
    20341 tatcagttgt ggtcaatata taagcgaatg taagcgattg ttgatgtcga aatggttgtt
    20401 ttctattcgg ttattgttaa a
//
LOCUS       DAZPJP010000061        20414 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPJP010000061 DAZPJP010000000
VERSION     DAZPJP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20414
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.061"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(249..1061)
                     /locus_tag="ACHU9J_002034"
     CDS             complement(249..1061)
                     /locus_tag="ACHU9J_002034"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1905573.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1323..2084
                     /gene="udp"
                     /locus_tag="ACHU9J_002035"
     CDS             1323..2084
                     /gene="udp"
                     /locus_tag="ACHU9J_002035"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1905574.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2225..3652
                     /gene="rmuC"
                     /locus_tag="ACHU9J_002036"
     CDS             2225..3652
                     /gene="rmuC"
                     /locus_tag="ACHU9J_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1905575.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3747..4502
                     /gene="ubiE"
                     /locus_tag="ACHU9J_002037"
     CDS             3747..4502
                     /gene="ubiE"
                     /locus_tag="ACHU9J_002037"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1905576.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4516..5121
                     /gene="ubiJ"
                     /locus_tag="ACHU9J_002038"
     CDS             4516..5121
                     /gene="ubiJ"
                     /locus_tag="ACHU9J_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1905577.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5118..6758
                     /gene="ubiB"
                     /locus_tag="ACHU9J_002039"
     CDS             5118..6758
                     /gene="ubiB"
                     /locus_tag="ACHU9J_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1905578.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6837..7106
                     /gene="tatA"
                     /locus_tag="ACHU9J_002040"
     CDS             6837..7106
                     /gene="tatA"
                     /locus_tag="ACHU9J_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1905579.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7110..7625
                     /gene="tatB"
                     /locus_tag="ACHU9J_002041"
     CDS             7110..7625
                     /gene="tatB"
                     /locus_tag="ACHU9J_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1905580.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7628..8404
                     /gene="tatC"
                     /locus_tag="ACHU9J_002042"
     CDS             7628..8404
                     /gene="tatC"
                     /locus_tag="ACHU9J_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1905581.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8446..9228
                     /gene="tatD"
                     /locus_tag="ACHU9J_002043"
     CDS             8446..9228
                     /gene="tatD"
                     /locus_tag="ACHU9J_002043"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1905582.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9225..9713)
                     /gene="rfaH"
                     /locus_tag="ACHU9J_002044"
     CDS             complement(9225..9713)
                     /gene="rfaH"
                     /locus_tag="ACHU9J_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1905583.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9880..11373
                     /gene="ubiD"
                     /locus_tag="ACHU9J_002045"
     CDS             9880..11373
                     /gene="ubiD"
                     /locus_tag="ACHU9J_002045"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1905584.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11420..12121
                     /gene="fre"
                     /locus_tag="ACHU9J_002046"
     CDS             11420..12121
                     /gene="fre"
                     /locus_tag="ACHU9J_002046"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1905585.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12311..13474)
                     /gene="fadA"
                     /locus_tag="ACHU9J_002047"
     CDS             complement(12311..13474)
                     /gene="fadA"
                     /locus_tag="ACHU9J_002047"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1905586.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13484..15673)
                     /gene="fadB"
                     /locus_tag="ACHU9J_002048"
     CDS             complement(13484..15673)
                     /gene="fadB"
                     /locus_tag="ACHU9J_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1905587.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15863..17194
                     /gene="pepQ"
                     /locus_tag="ACHU9J_002049"
     CDS             15863..17194
                     /gene="pepQ"
                     /locus_tag="ACHU9J_002049"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1905588.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17194..17808
                     /locus_tag="ACHU9J_002050"
     CDS             17194..17808
                     /locus_tag="ACHU9J_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1905589.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17847..19298
                     /gene="trkH"
                     /locus_tag="ACHU9J_002051"
     CDS             17847..19298
                     /gene="trkH"
                     /locus_tag="ACHU9J_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1905590.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19310..19855
                     /gene="hemG"
                     /locus_tag="ACHU9J_002052"
     CDS             19310..19855
                     /gene="hemG"
                     /locus_tag="ACHU9J_002052"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1905591.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            20233..>20414
                     /locus_tag="ACHU9J_002053"
     rRNA            20233..>20414
                     /locus_tag="ACHU9J_002053"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4788 a         5148 c         5556 g         4922 t
ORIGIN      
        1 atgatttata attaatgtga atgtgttgat aataattaat gtttgtttgt gaatgggagt
       61 tgatggtaaa tctctaaggt gtcttccatt gaagagactc atttatgact atgacttgcc
      121 gactttctta aggtcaaata gcggagtcgt aatctccaat caattcatac tgattatttt
      181 ttgggggaca gagatggcag caaaacgcac tcgtttactc accatctcaa atatagacta
      241 agaggaaact aactccgctt ctctccatac tgcttaaacc attccagcat acgctgccag
      301 ccatctttcg cagattcggc atgatagctc gggcgataat cagcgttgaa tgcatgcccg
      361 gcatccgggt acacgataat ctctgctttc gcattagcag cccgcagcgc ctggcgcatg
      421 gtttcaacgc tctcctgcgg aatgctgtta tcctgaccgc catataagcc gagaatcggc
      481 gcgttaagat cggttgcgat atcaacaggt tgtttcggtg aattcagcga cttgtcgccc
      541 gtcagtttgc cgtaccacgc cactgcagct tttagctgtg gattatgcgc ggcatacagc
      601 caggtgatac gtccacccca gcagaatccg gtgatcatta aacgatgaac atcgccgccg
      661 ttgcgggaag cccaactggc ggcatgatcg agatcggcca gcacctgcga gtcaggcact
      721 tttgctacca gaccgctaag caacgtgggg atatcggcaa aatcattcgg atcgccttcg
      781 cggaagtaaa gttcaggtgc gatagccaga tacccctcca gcgccagacg gcgacaaatg
      841 tcgcggatat gttcatgcac gccaaaaatt tcctgcacta caatgaccac tggcagtggg
      901 ccatcgcttt gctttggtct ggcatggtaa gcaggcatgt tatccccttg tgaagggata
      961 gaggtgaagc ccgccacaat cgcgtcgtcc agggtctgaa cgatggtcga agcgagaggc
     1021 gatgcagcag gtgcaaatcc agattgttgt gttgttgcca tggtattctc cgtaccctta
     1081 taaaaatgtt gcgcaatgtt aactatagtc agcatgcaac aaatcacatt gcctgaatcg
     1141 gctcatcttt tatgcagtcc tgcagaatga agagtgattt atgtgatttg aatcactttt
     1201 ggtgggtaaa tttatgcaac gcatttgcgt catggtgatg agtatcacga aaaaatgtta
     1261 aacccttcgg taaagtgtct ttttgcttct tctgactaaa ccgattcaca gaggagttgt
     1321 atatgtccaa gtctgatgtt tttcatctcg gcctcactaa aaacgattta caaggggcta
     1381 cgcttgccat cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg ctgatggata
     1441 agccggttaa gctggcatct caccgcgaat tcactacctg gcgtgcagag ctggatggta
     1501 aacctgttat cgtctgctct accggtatcg gcggcccgtc tacctctatt gctgttgaag
     1561 agctggcaca gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc
     1621 cgcatattaa tgtgggtgat gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga
     1681 gcctgcactt cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc
     1741 tggttgaagc tgcgaaatcc attggcgcga caactcacgt tggcgtgaca gcttcttctg
     1801 ataccttcta cccaggtcag gaacgttacg atacttactc tggtcgcgta gttcgtcact
     1861 ttaaaggttc tatggaagag tggcaggcga tgggcataat gaactatgaa atggaatctg
     1921 caaccctgct gaccatgtgt gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta
     1981 tcgttaaccg cacccagcaa gagatcccga atgctgagac gatgaaacaa actgaaagcc
     2041 aagcggtgaa aatcgtggtg gaagcggcgc gtcgtctgct gtaattctct tctcctgtct
     2101 gaaggccgac gcgttcggcc ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc
     2161 caactggacg tttgtacagc acaattctat tttgtgcggg taagttgttg cgtcaggagg
     2221 cgttgtggat ttctcaatca tggtttacgc agttattgcg ttggtgggtg tggcaattgg
     2281 ctggctgttt gccagttatc aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga
     2341 agagatggtc gcggagttaa gcgcggcaaa acaacaaatt acccaaagcg agcactggcg
     2401 tgcagagtgc gagttactca ataacgaagt gcgcagcctg caaagtatta acacctctct
     2461 ggaggccgat ctgcgtgaag taaccacgcg gatggaagcc gcacagcaac atgctgacga
     2521 taaaattcgc cagatgatta acagcgagca gcgcctcagt gagcagtttg aaaacctcgc
     2581 caaccgtatt tttgagcaca gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa
     2641 cagcctgttg tcgccgctac gtgaacaact ggacggtttc cgccgtcagg ttcaggacag
     2701 cttcggtaaa gaagcacaag aacgccatac cctgacccac gaaattcgca atctccagca
     2761 actcaacgcg caaatggccc aggaagcgat caacctgacg cgcgcgctga aaggcgacaa
     2821 taaaacccag ggcaactggg gcgaggtagt attgacgcgg gtgctggagg cttccggtct
     2881 gcgtgaaggg tatgaatatg aaacccaggt cagcatcgaa aatgacgccc gctcgcggat
     2941 gcagccggat gtcatcgtgc ggctgccgca gggaaaagat gtggtgatcg acgccaaaat
     3001 gacgctggtc gcctatgaac gctattttaa cgccgaagac gactacaccc gcgaaagcgc
     3061 gctacaggaa catatcgcgt cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta
     3121 tcaacagctg ccggggctgc gaactctgga ttacgtgctg atgtttattc ccgttgaacc
     3181 cgctttttta ctggcgcttg accgccagcc ggagctgatc accgaagcgt tgaaaaacaa
     3241 tatcatgctg gttagcccga ctacgctgct ggtggcgttg cgtactattg ccaacctgtg
     3301 gcgttatgag catcaaagcc gcaacgccca gcaaatcgcc gatcgtgcca gcaagctgta
     3361 cgacaagatg cgtttgttca tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc
     3421 gcaggataac taccgccagg caatgaaaaa actctcttca ggtcgcggaa atgtgctggc
     3481 gcaggcagaa gcgtttcgcg gtttaggagt agaaattaaa cgcgagatta atccggattt
     3541 ggctgaacag gcggtgagcc agaatgaaga gtatcgactt cggtcggttc cggagcagcc
     3601 gaatgatgaa gcttatcaac gcgatgatga atataatcag cagtcgcgct agcccattag
     3661 gggtagttaa gccgggtaga aatctagggc atcgacgccc aatctgttac acttctggaa
     3721 caattttttg atgagcaggc attgagatgg tggataagtc acaagaaacg acgcactttg
     3781 gttttcagac cgtcgcgaag gaacaaaaag cggatatggt cgcccacgtt ttccattccg
     3841 tggcatcaaa atacgatgtc atgaatgatt tgatgtcatt tggtattcat cgtttgtgga
     3901 agcgattcac gattgattgc agcggtgtac gccgtgggca gaccgtgctg gatctggctg
     3961 gtggcaccgg cgacctgaca gcgaaattct cccgcctggt cggagaaact ggcaaagtgg
     4021 tccttgctga tatcaatgaa tccatgctca aaatgggacg cgagaagctg cgtaatatcg
     4081 gtgtgattgg caacgttgag tatgttcagg cgaacgctga ggcgctgccg ttcccggata
     4141 acacctttga ttgcatcacc atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag
     4201 cactgcgttc aatgtatcgc gtgctgaaac ccggcggccg cctgctggtg cttgagttct
     4261 cgaagccaat tatcgagccg ctgagcaaag cctatgatgc atactccttc catgtgctgc
     4321 cgcgtattgg ctcactggtc gcgaacgacg ccgacagcta ccgttatctg gcagaatcca
     4381 tccgtatgca tcccgatcag gataccctga aagccatgat gcaggatgcc ggattcgaaa
     4441 gtgtcgacta ctacaatctg acggcagggg ttgtggcgct gcatcgtggt tataagttct
     4501 gacaggagac cggaaatgcc ttttaaacct ttagtgacgg caggaattga aagtctgctc
     4561 aacaccttcc tgtatcgctc acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa
     4621 gtattgcgcg tggaggtaaa aggcttttcg acgtcattga ttctggtgtt cagcgaacgc
     4681 caggttgatg tactgggcga atgggcaggc gatgctgact gcaccgttat cgcctacgcc
     4741 agtgtgttgc cgaaacttcg cgatcgccag cagcttaccg cactgattcg cagtggtgag
     4801 ctggaagtgc agggcgatat tcaggtggtg caaaacttcg ttgcgctggc agatctggca
     4861 gagttcgacc ctgcggaact gctggcccct tataccggtg atatcgccgc tgaaggaatc
     4921 agcaaagcca tgcgcggagg cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt
     4981 tatgtggcgg aagccattac tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc
     5041 tggtttgcgg aagagacggc tgccgtcgag cgtgctgttg atgccctgac caaacggctg
     5101 gaaaaactgg aggctaaatg acgccaggtg aagtacggcg cctatatttc atcattcgca
     5161 cttttttaag ctacggactt gatgaactga tccccaaaat gcgtatcacc ctgccgctac
     5221 ggctatggcg atactcatta ttctggatgc caaatcggca taaagacaaa cttttaggtg
     5281 agcgactacg actggccctg caagaactgg ggccggtttg gatcaagttc gggcaaatgt
     5341 tatcaacccg ccgcgatctt tttccaccgc atattgccga tcagctggcg ttattgcagg
     5401 acaaagttgc tccgtttgat ggcaagctgg cgaagcagca gattgaagct gcaatgggcg
     5461 gcttgccggt agaagcgtgg tttgacgatt ttgaaatcaa gccactggct tctgcttcta
     5521 tcgcccaggt tcataccgcg cgattgaaat cgaatggtaa agaggtggtg attaaagtca
     5581 tccgcccgga tattttgccg gttattaaag cggatctgaa acttatctac cgtctggctc
     5641 gctgggtgcc gcgtttgctg ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg
     5701 agtacgaaaa gaccttgatt gatgaactga atttgctgcg ggaatctgcc aacgccattc
     5761 agcttcggcg aaattttgaa gacagcccga tgctctacat cccggaagtt taccctgact
     5821 attgtagtga agggatgatg gtgatggagc gcatttacgg cattccggtg tctgatgttg
     5881 cgacgctgga gaaaaatggc accaacatga aattgctggc ggaacgtggc gtgcaggtgt
     5941 tcttcactca ggtctttcgc gacagctttt tccatgccga tatgcaccct ggcaacattt
     6001 tcgtaagcta tgaacacccg gaaaacccga aatatatcgg cattgattgc gggattgttg
     6061 gctcgctaaa caaagaagat aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc
     6121 gcgactatcg caaagtggca gagctacacg tcgattctgg ctgggtgcca ccagatacca
     6181 acgttgaaga gttcgaattt gccattcgta cggtctgtga acctatcttt gagaaaccgc
     6241 tggccgaaat ttcgtttgga catgtactgt taaatctgtt taatacggcg cgtcgcttca
     6301 atatggaagt gcagccgcaa ctggtgttac tccagaaaac cctgctctat gtcgaagggg
     6361 taggacgcca gctttatccg cagctcgatt tatggaaaac agcgaagcct ttcctggagt
     6421 cgtggattaa agatcaggtc ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc
     6481 cgttctgggt cgaaaaaatg ccagaactgc ctgaattggt ttacgacagt ttgcgccagg
     6541 gcaagtattt acagcacagt gttgataaga ttgcccgcga gcttcagtca aatcatgtac
     6601 gtcagggaca atcgcgttat tttctcggaa ttggcgctac gttagtatta agtggcacat
     6661 tcttgttggt cagccgacct gaatgggggc tgatgcccgg ctggttaatg gcaggtggtc
     6721 tgatcgcctg gtttgtcggt tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg
     6781 tcgtgtaacg tataatgcgg ccttgtttaa tcatcatcta ccacagagga acatgtatgg
     6841 gtggtatcag tatttggcag ttattgatta ttgccgtcat cgttgtactg ctttttggca
     6901 ccaaaaagct cggctccatc ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag
     6961 caatgagcga tgatgaacca aagcaggata aaaccagtca ggatgctgat tttactgcga
     7021 aaactatcgc cgataagcag gcggatacga atcaggaaca ggctaaaaca gaagacgcga
     7081 agcgccacga taaagagcag gtgtaatccg tgtttgatat cggttttagc gaactgctat
     7141 tggtgttcat catcggcctc gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa
     7201 cggtagcggg ctggattcgc gcgttgcgtt cactggcgac aacggtgcag aacgaactga
     7261 cccaggagtt aaaactccag gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc
     7321 tcactaacct gacgcccgaa ctgaaagcgt cgatggatga actacgccag gccgcggagt
     7381 cgatgaagcg ttcctacgtt gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca
     7441 tccataaccc ggtggtgaaa gataatgaag ctgcgcatga gggcgtaacg cctgccgctg
     7501 cacaaacgca ggccagttcg ccggaacaga agccagaaac cacgccagag ccggtggtaa
     7561 aacctgctgc ggacgctgaa ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac
     7621 cgtaaacatg tctgtagaag atactcaacc gcttatcacg catctaattg agctgcgtaa
     7681 gcgtctgctg aactgcatta tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc
     7741 caatgacatc tatcacctgg tatccgcgcc attgatcaag cagttgccgc aaggttcaac
     7801 gatgatcgcc accgacgtgg cctcgccgtt ctttacgccg atcaagctga cctttatggt
     7861 gtcgctgatt ctgtcagcgc cggtgattct ctatcaggtg tgggcattta tcgccccagc
     7921 gctgtataag catgaacgtc gcctggtggt gccgctgctg gtttccagct ctctgctgtt
     7981 ttatatcggc atggcattcg cctactttgt ggtctttccg ctggcatttg gcttccttgc
     8041 caataccgcg ccggaagggg tgcaggtatc caccgacatc gccagctatt taagcttcgt
     8101 tatggcgctg tttatggcgt ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct
     8161 gtgctggatg gggattacct cgccagaaga cttacgcaaa aaacgcccgt atgtgctggt
     8221 tggtgcattc gttgtcggga tgttgctgac gccgccggat gtcttctcgc aaacgctatt
     8281 ggcgatcccg atgtactgtc tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg
     8341 taaagggcga aatcgggaag aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga
     8401 ataaattcaa ccgcccgtca gggcggttgt catatggagt acaggatgtt tgatatcggc
     8461 gttaatttga ccagttcgca atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt
     8521 gacgcgggag ttaatgggct actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg
     8581 caaaagctgg cgcgtcagta ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac
     8641 agcagccagt ggcaagctgc gactgaagaa gcgattattg agctggccgc gcagccagaa
     8701 gtggtggcga ttggtgaatg tggtctcgac tttaaccgca acttttcgac gccggaagag
     8761 caggaacgcg cttttgttgc ccagctacgc attgccgcag atttaaacat gccggtattt
     8821 atgcactgtc gcgatgccca cgagcggttt atgacattgc tggagccgtg gctggataaa
     8881 ctgcctggtg cggttcttca ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg
     8941 gcgcatggaa tttatatcgg cattaccggt tgggtttacg atgaacgacg cggactggag
     9001 ctgcgggaac ttttgccgtt gattccggcg gaaaaattac tgatcgaaac tgatgcgccg
     9061 tatctgctcc ctcgcgatct cacgccaaag ccatcatccc ggcgcaacga gccagcccat
     9121 ctgccccata ttttgcaacg tattgcgcac tggcgtggag aagatgccgc atggctggct
     9181 gccaccacgg atgctaatgt caaaacacgg tttgggattg cgttttagag tttgcggaac
     9241 tcggtgttct tcacactgtg cttaatctct ttattaataa gattaagcaa tagcatggag
     9301 cgagcctcac catcgggttc ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta
     9361 ataatcacct tatctcccgg ataaggggtt gccggatcga caatgtcttt cggtttatat
     9421 accgatagct gatgaatcac cgccgatggg actatcgcta gcgacgcgcc aaagcgcacg
     9481 aagtggctga caccgcgggt cgcgttgata gtcgtggtat gaatcacttc tggatcaaat
     9541 tcgacaaaca ggtagttggg aaacaatggc tcactgactg cagtacgttt tccacgcacg
     9601 attttttcca gggtgatcat cggtgccagg caattcacag cctgtctctc aaggtgttcc
     9661 tgggcacgtt gaagttgccc gcgcttgcag tacagtaaat accaagattg cataatgact
     9721 cttatccgtt taatcggggc gcaaggatag caaaagcttt acgctaagtt aattatattc
     9781 cccggtttgc gttataccgt cagagttcac gctaatttaa caaatttaca gcatcgcaaa
     9841 gatgaacgcc gtataatggg cgtagattaa gaggctacaa tggacgccat gaaatataac
     9901 gatttacgcg acttcttgac gctgcttgaa cagcagggtg agctaaaacg tatcacgctc
     9961 ccggtggatc cgcatctgga aatcactgaa attgctgacc gcactttgcg tgccggtggg
    10021 cctgcgctgt tgttcgaaaa ccctaaaggc tactcaatgc cggtgctgtg caacctgttc
    10081 ggtacgccaa agcgcgtggc gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa
    10141 gttggtaaat tattggcgtt tctgaaagag cccgagccgc caaaaggttt ccgcgacctg
    10201 tttgataaac tgccgcagtt taagcaagta ttgaacatgc cgacaaagcg gctgcgtggt
    10261 gcgccctgcc aacaaaaaat cgtctctggc gatgacgtcg atctcaatcg cattcccatt
    10321 atgacctgct ggccggaaga tgccgcgccg ctgattacct gggggctgac cgtaacgcgc
    10381 ggcccgcata aagagcggca gaatctgggc atttatcgcc agcagctgat tggtaaaaac
    10441 aaactgatta tgcgctggct gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt
    10501 gcggcgcatc cgggcgaacg tttcccggtt tctgtggcgc tgggtgccga tcccgccacg
    10561 attctcggtg cagtcacccc cgttccggat acactttcag agtatgcgtt tgccggattg
    10621 ctgcgcggta ccaagaccga agtggtgaag tgtatctcca atgaccttga agtgcccgcc
    10681 agtgcggaga ttgtgctgga agggtatatc gaacaaggcg aaactgcgcc ggaagggccg
    10741 tatggcgacc acaccggtta ctataacgaa gtcgatagtt tcccggtatt taccgtgacg
    10801 catattaccc agcgtgaaga tgcgatttac cactccacct atactggtcg tccgccggat
    10861 gaacccgcgg tgctgggtgt cgcactgaac gaagtgtttg tgccgattct gcaaaaacag
    10921 ttcccggaaa ttgtcgattt ttacctgcca ccggaaggct gttcttatcg tctggcggta
    10981 gtgacaatca aaaaacagta cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg
    11041 ttcttacgcc agtttatgta cactaaattt gtgatcgttt gcgatgatga cgtcaacgca
    11101 cgcgactgga acgatgtgat ttgggcgatt accacccgta tggacccggc gcgggatact
    11161 gttctggtag aaaatacgcc tattgattat ctggattttg cctcgcctgt ctccgggctg
    11221 ggttcaaaaa tggggctgga tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg
    11281 ggacgtccca tcaaaaaaga tcctgatgtc gtcgcgcata ttgacgccat ctgggatgaa
    11341 ctggctattt ttaacaacgg taaaagcgcc tgatgcgcgt tagttttgcc ctatttattt
    11401 gatccgacag agaaagcgca tgacaacctt aagctgtaaa gtgacctcgg tagaagctat
    11461 cacggatact gtataccgtg tccgaatcgt accagacgcg gccttttctt ttcgtgctgg
    11521 tcagtatttg atggtagtga tggatgagcg cgacaaacgt ccgttttcaa tggcttcgac
    11581 gccggatgaa aaagggttta tcgagctgca tattggcgct tctgaaatca acctttacgc
    11641 gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc gtggtcgata ttccccacgg
    11701 agaagcgtgg ctgcgcgatg atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg
    11761 gttctcctat gcgcgctcga ttttattgac ggcgttggcg cgtaatccca atcgcgatat
    11821 caccatttac tggggtgggc gtgaagagca acatctgtat gatctctgcg agcttgaggc
    11881 gctttcgctg aagcatcctg gtctgcaagt ggtgccggtg gttgaacaac cggaagcggg
    11941 ctggcgtggg cgtactggca ccgtgttaac ggcggtcttg caggatcatg gtacgctggc
    12001 agagcatgat atctatattg ccggacgttt tgagatggcg aaaattgctc gtgacctgtt
    12061 ttgcagtgag cgtaatgcgc gggaagatcg tctgtttggc gatgcgtttg catttatctg
    12121 agatataaaa acccgcccct gacaggcggg aagaacggca actaaaactt tattcagtgg
    12181 catttagatc tatgtcgcat ccggcaatcg gtgcacgatg cctgatgcga cgctggcgcg
    12241 tcttatcagg cctacattgg tgcaggatcg gtagaccgga taaggcgttc acgccgcatc
    12301 cggcaagtgg ttaaacccgc tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca
    12361 tcgtcgccag accaaactga acgtctttgt gttccatcag attcagcaac gtggtgctga
    12421 tacgcgcacc ggaacaaccc agcggatgac ccagcgcgat cgcaccacca ttgaggttga
    12481 tcttctcgtc aatctgctcc attagcccga gatctttaat gcacggcagg atctgcgcgg
    12541 caaaggcttc gttcatctca aacacgccga tatcgctggc agaaagcccc gcttttttca
    12601 gcgccagctt cgaggcagga accgggccgt aacccataat cgacggatca caaccaacga
    12661 ccgccatcga acgcacacgg gcgcgcggct taagacctaa ttcacgggcg cggctttcac
    12721 tcatcaccag catggccgcg gcaccatcag aaagtgcaga agatgtaccc gccgttaccg
    12781 taccgcttac tggatcaaac gccggacgca gcgtggcgag ggcttccacg gtggtttccg
    12841 ggcgaatcac ttcgtcgtaa ttaaactgct tcaggacgcc gtcggcatcg tgaccaccgg
    12901 tcgggatgat ttcattttta aatgcgcccg actgcgtggc ggcccaggcg cgggcgtgtg
    12961 accgcgcggc aaaggcatcc tgcatttcac ggctgatacc gtgcatacgc gccagcattt
    13021 ctgccgttaa gcccatcatg cccgccgctt tggcgacatt gcggctcagg ccggggtgaa
    13081 aatcgacgcc gtgactcatc ggcacatggc ccatatgctc cacgccgcca accagacatg
    13141 cctgcgcatc gccagtcatg atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac
    13201 cacacaagcg attgacggta accgccggga cagagtgcgg cacttctgcc agcagcgccg
    13261 cgttacgggc gatattaaaa ccctgctcca gcgtctgctg cacacaaccc cagtaaatat
    13321 cgtcgagggc cgccgcttcc agcgccgggt tacgcgccag caggctacgc attaaatgag
    13381 cggagagatc ttctgcacgc acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg
    13441 tgcgaattgc atcgacaatg acaacctgtt ccattgtgac tccttaagcc gttttcaggt
    13501 cgccaaccgg acgggctggc tcaaccggag gatagtacgg ttcgttatga cgcgctttat
    13561 tacgcagacc ttccggcact tcatacagcg ggccgaggtg ctgatattgc tgtgccatat
    13621 cgaggtattt tgcgctaccg agggtgtcca gccagcggaa cgcgccgccg tggaacggag
    13681 ggaagcccag gccgtagacc agcgccatat ccgcttccgc cggagtggcg ataatgccct
    13741 cttccagaca gcgcaccact tcgttgacca tcgggatcat catgcgggcg ataatctctt
    13801 cttcgctgaa atcgcgcttc ggctggctca cttctgccag caggtcttca acggcggcgt
    13861 cttcttcttt cttcggctta cctttgctgt cttctttata acgccagaaa ccgaggccgt
    13921 tcttctgacc aaagcggttg gcatcaaaca gcgcgtcgat ggcatcgcgg taatcttttt
    13981 gcatccgctg cgggaagcct gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc
    14041 ccacaacgtc cagcagatat gccgggccca tcggccagcc aaactgtttt tccatcactt
    14101 tgtcgatctt gcggaaatcc gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg
    14161 ggaacagcac gcggttaaca aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc
    14221 ccatcttgct cgcccaggcg acaactttcg cgatggtttc gtcggagctt ttctcgccgc
    14281 gaataatttc taccaacggc attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt
    14341 tttccgggcg ttccagcgcg ttggccagtt cgctgatagg aatggttgaa gtgttagacg
    14401 ccagcacggt atcctggcgt actttttgtt cggtttctgc cagtacggct tttttcactt
    14461 tcgggttttc aacaaccgct tctaccacaa tatccacgcg gtcaaatccg gcgtagtcga
    14521 gcgttgggtg gatcgtggag atcacgccag ccagtttcag accatcgatc ttgccgcgct
    14581 caagctgctt gttcagcagt ttcgcggctt cggtcatgct gagggttaac gacttgtcgt
    14641 tgatatcttt catgacaacc ggcacgcctt tccacgcgga ctggtaagcg ataccgccac
    14701 ccatgatgcc tgcgcccagc acagcagcct gtttcggggt ttcaacgtct ttggtgagtt
    14761 tcttcgcttt gccttttaca tattgatcgt taaggaaaat gccgaccagt gcgcgagctt
    14821 cgttggtatg cgccagcggg acaaaacttt tgttttccag atttaaggct tcttcacgac
    14881 caaaacgggc cgcagcttca atggttttta ctgcggtgat gggggccgga taatgtttcc
    14941 ccgccgtttg cgcgaccatt cctttagcga tggtgaagct catggtggct tcaatcttgc
    15001 tcagttttag cggttccagc ttcggctggc gtttcgcttt ccagtcgagg tcgccgttaa
    15061 tggcctggcg taaaaccgcc tttgcgcctt caaccagttt ttctgctttg actaccccat
    15121 ccaccagacc gattttcagc gcctgatccg cgccgacatc tttaccggcg gcaatgattt
    15181 ccagcgcact gtcagcgccc agcatacgtg gcatacgtac agaaccgcca aagcccggca
    15241 tgatgcccag tttggtttcc ggcagaccga tgcgcagatc cggcgtcgcc agacgataat
    15301 cggtcgccag cacgcattcg cagccaccac ccagcgcata gccgttgacg gcagcaatgg
    15361 tcggcaccgg cagatcttcc aggcgattaa acacgctatt ggcaaagtgc agccactgac
    15421 ttaactgttc ttcaggaacg aggaacaggg acaaaaattc ggtgatatca gcaccgacga
    15481 taaaggctgc tttgttcgaa cgcagcagca gcccttttag atctgattgc tgttccagca
    15541 cgccgatggc ctcgccgagg ctggcgacgg tcgcagtgtc gagtttatta actgaacctg
    15601 gggcatcaaa taccagttcg gcaatgccat cttccagcca gtcaaggtac agggtgtcgc
    15661 ctttgtaaag catgtcagtc tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc
    15721 gaagtgtgta ttttgtgtta aaaatatgca aataaaagat taaagaaatg ccgatctgat
    15781 cacgctcggc agaaatcacg ctctggatga acgatgtgct aagatgcgga gacttaaggt
    15841 caaaaaaaca gaagggtaaa aaatggaatc actggcctcg ctctataaaa atcatatagc
    15901 taccttacaa gaacggactc gcgatgcgct ggcgcgcttc aagctggatg cattacttat
    15961 tcactccggc gagctgttca acgtttttct cgacgatcat ccctatccgt ttaaagtgaa
    16021 cccgcaattc aaagcgtggg tgccggtaac tcaggtgcca aactgctggt tgctggtgga
    16081 tggcgtgaac aagccgaaac tgtggttcta tctgccggtt gattactggc acaacgtcga
    16141 accgctgccg acctccttct ggactgaaga tgtggaagtg atcgcactgc cgaaagccga
    16201 tggcattggt agcctgctgc ccgctgcgcg cggcaatatc ggttatatcg gtccggtgcc
    16261 ggaacgtgcg ctgcaactgg gtattgaggc cagcaacatc aacccgaaag gggtgatcga
    16321 ctacctgcat tactaccgct ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc
    16381 gcagaaaatg gcggtcaacg gtcatcgtgc ggcagaagaa gcgttccgtt ctggcatgag
    16441 cgagttcgat atcaacatcg cctatctgac cgcgaccggt catcgtgata ccgacgtacc
    16501 ttacagcaac attgtggcgc ttaacgaaca cgcttcggtg ctgcattaca ccaaactgga
    16561 tcatcaggca ccggaagaga tgcgcagctt cctgctggat gccggggccg aatataacgg
    16621 ctatgcggct gacctgactc gtacctggtc ggcaaaaagc gacaacgact acgcacagct
    16681 ggtgaaagac gtaaatgatg aacaactggc gctgatcgcc accatgaaag caggcgttag
    16741 ctatgtggat taccacatcc agttccatca gcgcatcgcc aaactgctgc gtaaacatca
    16801 aatcatcacc gatatgagtg aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat
    16861 gccgcatggt atcggtcatc cgctgggcct gcaggtgcat gacgtagccg gttttatgca
    16921 ggatgatagc ggtacacacc tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg
    16981 tattctccag ccgggtatgg tgttaaccat cgaaccgggt atctacttca ttgaatcgct
    17041 actggcaccg tggcgtgaag ggcagttcag caagcacttc aactggcaga aaattgaagc
    17101 actgaaaccg ttcggcggca ttcgtatcga agacaacgtg gtgatccacg aaaacaacgt
    17161 ggaaaacatg acccgggatc tgaaactggc gtgatggaaa gctggttaat tcctgcggca
    17221 ccggtcacgg tcgttgaaga gatcaaaaaa agccgcttca taacgatgtt ggcgcatacc
    17281 gatggcgttg aggcggcgaa agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc
    17341 cgccaccatt gcgtggcgtg ggtggcgggt gcgccggatg attctcaaca gctgggtttc
    17401 tctgacgacg gggagccggc aggaacggca ggtaaaccga tgctcgccca gctaatgggc
    17461 agcggcgtcg gggaaattac cgctgtggta gtgcgctact acggcggcat attgcttggc
    17521 accggtgggt tagtgaaagc gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg
    17581 acccaacgca agacgccatt aaccgaatat actttgcaat gtgaatatca tcagttaacc
    17641 ggcattgaag cgttgctggg gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca
    17701 ttcgttctgc tgcgggtggc gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg
    17761 gcggatttta gccgtggttc attgcaattg ttagcgattg aagaataatc cccacttcgt
    17821 tttgcagaac taaggaagcg gcagagatgc attttcgcgc cattacccga atcgttggac
    17881 tactggtcat cttattttca gggaccatga ttatccctgg gctggtagca ctcatctacc
    17941 gggatggagc gggccgcgct tttacccaga ccttttttgt cgccctcgcc attggttcta
    18001 tgctgtggtg gccgaaccgt aaagagaagg gcgaactgaa atcccgtgag gggtttctga
    18061 tagtggtgct gttctggacc gtgctgggta gtgtcggtgc gctccctttt atcttctcgg
    18121 aaagcccgaa cctcacgatt accgatgcgt tttttgaatc tttctctggc ctgaccacca
    18181 ccggggccac tacgctggtg gggctggatt cgctccctca tgccatcctc ttttatcgcc
    18241 agatgctgca atggtttggc gggatgggga tcatcgtgtt agcggttgcg atactgccta
    18301 tcctcggcgt gggtgggatg cagctctatc gcgcagaaat gcccggcccg ctgaaagata
    18361 acaaaatgcg cccgcgaatt gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc
    18421 tgaccgtcgc ctgtgcgctg gcgttgtggt ttgccggaat ggatgccttt gatgccatcg
    18481 gccatagctt tgcgactatc gctattggcg gcttctcgac acatgatgcc agtatcggtt
    18541 atttcgacag cccgactatt aacactatca ttgctatctt cctgctgatc tccggctgta
    18601 actacggtct gcacttttca ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc
    18661 cggaatttcg catgtttatc ggcgtacagt ttacgctggt ggttatttgt acactcgtac
    18721 tgtggtttca taatgtctac agttcggcgc tgatgacaat taaccaggcg tttttccagg
    18781 tggtatcgat ggcgacaacc gccgggttta cgactgacag cattgcccgc tggccgctct
    18841 ttttgccggt actgctttta tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg
    18901 gtggcctgaa agtgatccgc atcctgctgc tgtttaagca ggggaaccgt gagctgaaac
    18961 gactggtgca tccgaacgcc gtctatagca ttaagctggg gaatcgcgca ctgccggaac
    19021 gtatcctcga agccgtttgg ggatttttct ccgcctatgc attggtgttt attgtcagta
    19081 tgctggcgat tatcgccacg ggcgtggatg acttttctgc ctttgcttca gttgttgcga
    19141 cattgaataa cctggggcca gggcttggcg tggttgctga taactttacc agtatgaacc
    19201 cggtggctaa atggatcctg attgccaaca tgctgtttgg tcgtctcgag gtctttacat
    19261 tgctggtgct ctttaccccg actttctggc gtgaatgatg gagtaatacg tgaaaacatt
    19321 aattcttttc tcaacaaggg acggacaaac gcgcgagatt gcctcctacc tggcttcgga
    19381 actgaaagaa ctggggatcc agacggatgt cgccaatgtg caccgcattg aagaaccaca
    19441 atgggaaaat tatgaccgtg tggtcattgg tgcttctatt cgctatggtc actaccactc
    19501 tgcgttccag gaatttgtca aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt
    19561 ttattccgta aacctggtgg cgcgcaaacc ggagaagcgt actccacaga ccaacagcta
    19621 cgcgcggaag tttctgatga actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg
    19681 ggcgctgcgt tacccacgtt atcgctggta cgatcgtttt atgatcaagc taattatgaa
    19741 gatgtcaggc ggtgaaacgg atacgcgcaa agaagttgtc tataccgatt gggagcaggt
    19801 ggcgaatttc gcccgagaaa tcgcccagtt aaccaacaaa ccgacgctga aataagcata
    19861 aagaataaaa aatgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata
    19921 actccctata atgcgccacc actgacacgg aacaacggca aacacgccgc cgggtcagcg
    19981 gggttctcct gagaattccg acagagaaag caaaaataaa tgcttgactc tgtagcggga
    20041 aagcgtatta tgcacacccc gcgccgctga gaaaaagtga agcggcactg ctctttaaca
    20101 atttatcaga caatctgtgt gggcactcga agatacggat tcttaacgtc gcaagacgaa
    20161 aaatgaatac caagtctcaa gagtggacac gtaattcatt acgaagttta attctttgag
    20221 catcaaactt ttaaattgaa gagtttgatc atggctcaga ttgaacgctg gcggcaggcc
    20281 taacacatgc aagtcgaacg gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg
    20341 acgggtgagt aatgtctggg aaactgcctg atggaggggg ataactactg gaaacggtag
    20401 ctaataccgc ataa
//
LOCUS       DAZPJP010000062        20183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPJP010000062 DAZPJP010000000
VERSION     DAZPJP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.062"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..882
                     /gene="yieE"
                     /locus_tag="ACHU9J_002054"
     CDS             1..882
                     /gene="yieE"
                     /locus_tag="ACHU9J_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1905592.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1087..2556
                     /gene="lysP"
                     /locus_tag="ACHU9J_002055"
     CDS             1087..2556
                     /gene="lysP"
                     /locus_tag="ACHU9J_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1905593.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2848..4839
                     /gene="cirA"
                     /locus_tag="ACHU9J_002056"
     CDS             2848..4839
                     /gene="cirA"
                     /locus_tag="ACHU9J_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR1905594.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4871..5707)
                     /gene="yeiG"
                     /locus_tag="ACHU9J_002057"
     CDS             complement(4871..5707)
                     /gene="yeiG"
                     /locus_tag="ACHU9J_002057"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1905595.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5965..6633
                     /gene="folE"
                     /locus_tag="ACHU9J_002058"
     CDS             5965..6633
                     /gene="folE"
                     /locus_tag="ACHU9J_002058"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1905596.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6650..7807
                     /gene="yeiB"
                     /locus_tag="ACHU9J_002059"
     CDS             6650..7807
                     /gene="yeiB"
                     /locus_tag="ACHU9J_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1905597.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7991..8989
                     /gene="galS"
                     /locus_tag="ACHU9J_002060"
     CDS             7991..8989
                     /gene="galS"
                     /locus_tag="ACHU9J_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1905598.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9269..10267
                     /gene="mglB"
                     /locus_tag="ACHU9J_002061"
     CDS             9269..10267
                     /gene="mglB"
                     /locus_tag="ACHU9J_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1905599.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10328..11848
                     /gene="mglA"
                     /locus_tag="ACHU9J_002062"
     CDS             10328..11848
                     /gene="mglA"
                     /locus_tag="ACHU9J_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1905600.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11864..12874
                     /gene="mglC"
                     /locus_tag="ACHU9J_002063"
     CDS             11864..12874
                     /gene="mglC"
                     /locus_tag="ACHU9J_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1905601.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13001..14236)
                     /gene="preA"
                     /locus_tag="ACHU9J_002064"
     CDS             complement(13001..14236)
                     /gene="preA"
                     /locus_tag="ACHU9J_002064"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1905602.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14230..15468)
                     /gene="preT"
                     /locus_tag="ACHU9J_002065"
                     /pseudo
     CDS             complement(14230..15468)
                     /gene="preT"
                     /locus_tag="ACHU9J_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(15662..15901)
                     /locus_tag="ACHU9J_002066"
     CDS             complement(15662..15901)
                     /locus_tag="ACHU9J_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1905603.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15904..16623)
                     /gene="sanA"
                     /locus_tag="ACHU9J_002067"
     CDS             complement(15904..16623)
                     /gene="sanA"
                     /locus_tag="ACHU9J_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1905604.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16773..17657)
                     /gene="cdd"
                     /locus_tag="ACHU9J_002068"
     CDS             complement(16773..17657)
                     /gene="cdd"
                     /locus_tag="ACHU9J_002068"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1905605.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17787..18482)
                     /locus_tag="ACHU9J_002069"
     CDS             complement(17787..18482)
                     /locus_tag="ACHU9J_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1905606.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18479..18877)
                     /locus_tag="ACHU9J_002070"
     CDS             complement(18479..18877)
                     /locus_tag="ACHU9J_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1905607.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19116..20063
                     /gene="dusC"
                     /locus_tag="ACHU9J_002071"
     CDS             19116..20063
                     /gene="dusC"
                     /locus_tag="ACHU9J_002071"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1905608.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4939 a         4989 c         5284 g         4971 t
ORIGIN      
        1 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
       61 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
      121 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
      181 gaacacgggc ggctgctcta tccgcgtgcg ttggcattgc ttgaacaggc ggttgaaatc
      241 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
      301 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
      361 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
      421 attggcttta ttgaaggacc gtgccacagc actgaaatca tttctgaacc gtggctggaa
      481 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta
      541 gaacagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
      601 gtcgattatc tgttgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
      661 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgt
      721 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctcccgcgc
      781 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
      841 cgctttctgg actattgcga tcccgcgaat gtaccgcgtt aaggtgctgt acaagaacat
      901 gctggtgctg tgtcgatttc gtgacgcagc gccctcagca tgcattcgcc agaaaagaga
      961 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
     1021 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
     1081 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
     1141 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
     1201 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
     1261 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
     1321 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
     1381 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacttggt tgcagcacag
     1441 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
     1501 ggcgttatct tcctgctgaa ctacatctca gttcgtggct ttggtgaagc ggaatactgg
     1561 ttttcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
     1621 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
     1681 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
     1741 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
     1801 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt cgcgatcctg
     1861 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
     1921 atcagcgtca gtccgttcac cttggtgttc cagcacgcgg gtctgctctc tgcggcggcg
     1981 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
     2041 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
     2101 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
     2161 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
     2221 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
     2281 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
     2341 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
     2401 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
     2461 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
     2521 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
     2581 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
     2641 ttaacatttg gattgataac tgttatcgtt tgcattatcg ttacgctaca atcaaaaaag
     2701 gctgacaaat cagaggctgg tccggctttc tgggatgatc acctgcatac aaataagtcc
     2761 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
     2821 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
     2881 ctgtgtttgt ccgctatttc ttgtgtatgg cctgtgttag cggtcgatga cgatggcgaa
     2941 acgatggttg tcactgcatc ttccgttgaa caaaacctta aagatgcacc cgccagtatc
     3001 agcgtcatta cccaggaaga tctgcagcga aaaccggtac agaatctgaa ggatgtcctc
     3061 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
     3121 cgtggtctgg acagcagcta taccctgatt ctcgtcgacg gtaaacgcgt gaactcccgc
     3181 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
     3241 cgtattgaag tggtccgtgg cccgatgtcg tcgctgtacg gttccgatgc gctcggcggt
     3301 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
     3361 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
     3421 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
     3481 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
     3541 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
     3601 actgccggat acggtttcga ccgtcaggat cgtgattccg actcgctgga caaaaaccgc
     3661 ctggaacgcc agaactactc cgtcagccat aatgggcgtt gggattacgg caccagcgaa
     3721 ctgaaatact acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
     3781 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
     3841 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
     3901 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
     3961 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
     4021 gaacactgga gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa
     4081 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
     4141 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
     4201 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaaggggtt
     4261 gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
     4321 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
     4381 ggacggcgca taccggtatt tagctactac aacgttaaca aagctcgtat tcagggcgtg
     4441 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
     4501 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
     4561 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
     4621 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
     4681 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
     4741 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
     4801 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
     4861 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
     4921 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
     4981 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
     5041 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
     5101 gcgcgcaact atcccattcc agccatgcat ttttgtcctc acctaaatag gtgctaaacg
     5161 ctttgattcc ccacgggacg ctgcacggat tcacaattgg cgcaaaggcc gaaacgctgg
     5221 tgtatttacc cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac
     5281 cgctaatggc gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat
     5341 cgcgcagata atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat
     5401 aaaagcctgc gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg
     5461 ggctggtgtc tggcatcacc agtacaatcc ccagttctgc cgccacgcgc tgggcacccg
     5521 ccttggtggt gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg
     5581 gcggggtgtg atcacgaggg ggtgggagaa agatactgaa cgtcatcggg cagtttaagg
     5641 tactggagtc gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca
     5701 tttccatgcg tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa
     5761 tgtgctgtga gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg
     5821 aaagtcagcg gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca
     5881 cgcacaataa tcaggctgta aatcagctta ataactttgc ccccacgcag ggcggaggcg
     5941 tcacacctgc aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat
     6001 gaagcgttag ttgcgcgagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat
     6061 aacgaaacgc gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat
     6121 ctcgacctgg ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc
     6181 gatgaaattt tctccggtct ggattacgcc aacttcccga aaatcaccct cattgaaaac
     6241 aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa
     6301 caccattttg ttaccatcga tggcaaagcg acggtggctt atatcccgaa agattcggtg
     6361 atcggtctgt caaaaattaa ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag
     6421 gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg
     6481 gctgtctcca ttgatgcggt gcattactgc gtgaaagcgc gtggcatccg cgatgcaacc
     6541 agtgccacaa caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac
     6601 gagtttctgc gcgctgtgcg tcatcacaac tgattaaaag gcaggaacca tggagcgcaa
     6661 cgtaacgctc gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag
     6721 cgcctttggg ctaccaaagg cggcttatct caatcccgcc tggtacggcg ctattacgcc
     6781 gcaggatgca tggacctggg catttctcga tctcatcggc caggtgaaat tcctcacgct
     6841 ttttgcgctg ctgtttggtg cgggcctgca aatgttgctg ccccgtggca gacgctggat
     6901 ccagtcgcgg ttaacgctgt tagtcttgct gggttttatt cacggtttat tgttctggga
     6961 cggcgatatt ctgctggctt acgggctggt gggcttaatc tgctggcggc tggtgcgcga
     7021 tgcgccatcg gtaaaaagcc tgtttaatac aggcgtcatg ctttatctgg tggggcttgg
     7081 cgttttgctg ttattggggc tgatttccga cagccagacc agccgcacct ggacgccgga
     7141 tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg aagcgatcag
     7201 taatcgtgcc gatggtgttg gcaacagttt actggcactg ggcgcacagt atggctggca
     7261 actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc tgaaagggca
     7321 gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg gggtgaccat
     7381 taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt
     7441 cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct atgcgtcgct
     7501 gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga tcgcctgcgt
     7561 cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca tgctttttta
     7621 ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg ttattccggt
     7681 atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc aggggccggt
     7741 ggaatggctc tggcgtcagt taactttgcg tgctgccgga acggcaatat ctaaaacatc
     7801 aagataacga tctggatcac attcgttaac aaaacggctg taaccgtttc cattgctgtg
     7861 actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca agcagtcatt
     7921 tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag cgcgttaggc
     7981 tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg tcagtgccga
     8041 cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa acgccaatgc
     8101 ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg acgtttctga
     8161 tgcgtttttc ggcgcgctgg taaaagcggt ggatctagtc gctcagcagc atcagaaata
     8221 cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt
     8281 aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg acgatgaact
     8341 ggcgcaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg tgccggggta
     8401 cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga cgcgcatgtt
     8461 gctgaataac ggtcatcaac gtattggtta tctttcttcc agtcacggca ttgaagatga
     8521 cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta ttccgccgga
     8581 aagctggatt ggcactggta cgccggacat gccgggcggt gaggcggcga tggttgaact
     8641 gctggggcgc aatctacaac ttaccgctat atttgcttat aacgacaata tggccgctgg
     8701 cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct caatcatcgg
     8761 tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc
     8821 cattgcttca atggctaaat tagccaccga actggccttg cagggggcag caggcaatat
     8881 tgatcctcgt gccagccact gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac
     8941 gcgccagaat gcggcggcga tcactaactc aacaaatcag gcgatgtaac cgctttcaat
     9001 ctgtgagtga tttcacagta tcttaacaat gtgatagcta tgattgcacc gttttaacgt
     9061 tgtaacccgt atgtaacagt gaataatcac tttcaccgag gtaatagcgt cataacaaca
     9121 attaaagccg ttttctggag cgttaccggg catggaagaa cgaatttaaa aaagtgagct
     9181 tcggcgttca gtaacacttc attaactcta ctgccccgcc gagcatttat ctcaagcact
     9241 accctgcata agaaaaaccg gagataccat gaataagaag gtgttaaccc tgtctgctgt
     9301 gatggccagc atgttattcg gtgccgctgc acacgctgct gatactcgca ttggtgtaac
     9361 aatctataag tacgacgata actttatgtc tgtagtgcgc aaggctattg agcaagatgc
     9421 gaaagccgcg ccagatgttc agctgctgat gaatgattct cagaatgacc agtccaagca
     9481 gaacgatcag atcgacgtat tgctggcgaa aggggtgaag gcactggcaa tcaacctggt
     9541 tgacccggca gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg tgccggtggt
     9601 tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag cctactacgt
     9661 tggcactgac tccaaagagt ccggcattat tcagggcgat ttgattgcta aacactgggc
     9721 ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt cagttcgtac tgctgaaagg
     9781 tgaaccgggc catccggatg cagaagcacg taccacttac gtgattaaag aattgaacga
     9841 taaaggcatc aaaactgaac agttacagtt agataccgca atgtgggaca ccgctcaggc
     9901 gaaagataag atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg aagtggttat
     9961 cgccaacaac gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac acaacaagtc
    10021 cagcattccg gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg tgaaatccgg
    10081 tgcactggcg ggcacagtac tgaacgatgc taacaaccag gcgaaagcga cctttgatct
    10141 ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc accaactgga aaatcgacaa
    10201 caaagtggtc cgcgtacctt atgttggcgt agataaagac aacctggctg aattcagcaa
    10261 gaaataagac tgattgggcg cagtcgttta ctgcgccctt attgtatggc caacaaggta
    10321 aaattatatg gtcagctcaa cgactccgtc ttccggggaa tacttgttgg aaatgagcgg
    10381 tatcaacaag tcctttcctg gtgttaaggc acttgataac gttaatttaa aattccggcc
    10441 acattctatc catgcattaa tgggggaaaa cggtgcagga aaatcgacat tattaaaatg
    10501 cctgtttggt atttatcaaa aagactccgg caccatttta ttccagggta aagagatcga
    10561 tttccattcc gccaaagagg cactggaaaa tggtatttcg atggtacacc aggagttaaa
    10621 cctggtatta caacgttcgg tgatggataa catgtggctg gggcgatatc ccaccaaagg
    10681 catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg atgaactgga
    10741 tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa tgcagatgat
    10801 cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg attatggatg aaccgacttc
    10861 ttcgttaacc gaaaaagagg tcaatcatct gttcactatt attcgtaaat taaaagagcg
    10921 cggctgcggt attgtttata tctcgcataa aatggaagaa atcttccagt tatgtgatga
    10981 agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag gactgacgat
    11041 ggacaagatc atcgccatga tggttgggcg ttctcttaat cagcgttttc ctgacaaaga
    11101 aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc gccagccgtc
    11161 gattcgcgat gtctcgtttg atctgcataa aggggagatc ctcggtattg ccgggctggt
    11221 gggggcgaaa cgtaccgata ttgttgagac gttatttggt attcgcgaga aatcggctgg
    11281 caccattacg ttgcacggca aacagatcaa taaccataat gccaacgaag ccataaacca
    11341 cggatttgca ctggtaactg aggagcgccg ctcaacggga atttatgcct atctggatat
    11401 tggttttaac tcgttaattt ccaatattcg caactacaaa aataaagttg gtttactgga
    11461 taactcgcgg atgaaaagcg atacccagtg ggtgattgat tcgatgcggg taaaaacgcc
    11521 gggtcatcgg acgcaaattg gttcgctctc cggtggtaat cagcaaaagg tgattattgg
    11581 tcgctggcta ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc gcggtattga
    11641 tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga aaggcaaggg
    11701 gattattatt atctcctctg aaatgcctga gttgttaggg ataacagacc gtattctggt
    11761 catgagcaat ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc aaaacgaaat
    11821 tctgcgtctt gcgtctttgc acctttaaga ttaggggctt cccatgagtg cgttaaataa
    11881 gaaaagtttt cttacttacc tgaaagaggg cggtatttac gtcgttcttt tagttttgct
    11941 ggcgattatt attttccagg acccaacatt tttaagtctg ttgaacttaa gtaatattct
    12001 cacccagtca tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa ttgtcaccca
    12061 ggggaccgat ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg ctgcgacatt
    12121 attgcagtcc atggataacg ccaacaaagt gttcccggaa atggcgacga tgccgattgc
    12181 gctggttatt ctgattgtct gtgccattgg tgcggtgatc ggtttgatca acggtctgat
    12241 tatcgcttat ctcaacgtga cgccgttcat taccacgctc ggcacgatga tcatcgtcta
    12301 tggcatcaac tcgctctatt acgactttgt cggggcgtcg ccaatttctg gttttgacag
    12361 tggcttctct acctttgctc agggctttgt cgcgctgggg agtttccgtc tctcttacat
    12421 caccttctac gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca aaacccgctt
    12481 cggtaagaac atttttgcca ttggcggtaa cccggaagcg gcaaaagtat ctggtgtcaa
    12541 cgtcggcctg aacctgctga tgatctacgc gttgtctggc gtgttctatg cctttggcgg
    12601 gatgttagaa gccggacgta tcggctctgc caccaacaac ctcggcttta tgtatgagct
    12661 ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg tggggacggt
    12721 gattggcgtg gtgaccgggg tgattatttt taccgtcatc aactatggcc tgacgtatat
    12781 cggcgtaaac ccatactggc agtacatcat caaaggggcg attattatct tcgccgtagc
    12841 gctggattca ctgaaatacg cgcgtaagaa atgatgtgaa tgtggtccgc attcggtcat
    12901 aaacgcacat aacatcatga tctgtgtggt tttgtaggcc ggataaggcg ttcacgccgc
    12961 atccggcaac aggaaccaaa tgccggatgt gacgcgggtt ctacaacgtt accgggtgtt
    13021 ctttctcgcc tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt
    13081 gaccacaaag cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct
    13141 cgctccattc catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc
    13201 caacacattt gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg
    13261 ccgggacaat attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat
    13321 cggcgagata atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca
    13381 taatgccggt ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatcct
    13441 cccaggtttc aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc
    13501 gcatttgctg aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa
    13561 tacttgattt tccgttaacg atcggcatac cgattttctg attaagatcg atattggtga
    13621 tggatttaac ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg
    13681 ccacttcgca catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac
    13741 cccgtttcac tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac
    13801 ccatcgcatg agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag
    13861 cttcttgcac caggcgcgcc agctcctccc attgctgctc attttccccc atgatcgaag
    13921 cgatcaatac tttgtccggg taatcttctt tcagccgacg cagggcggcc agattctcct
    13981 ccagcggatg ctcggcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt
    14041 tcaccagatg atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg atcgttttaa
    14101 acaccacacc gccccaacct gtgtcgtagg ctttggcaca catctcatag cagttgccta
    14161 ccggcgaaga agagaggcag aacgggttgg gaaacttcac gccgcaaaaa gtaatcgaaa
    14221 gatctttcgt taacatgagc aagctccctc taaatagtga tgaatcgcct cggcggcttc
    14281 tttcccggtt ttcacggcat agaccacggt tttgtctccc tcaacaatat cgccagcagc
    14341 aaagacttgc gggtcgcggg tctggtaatt ttgtgttttg atggtgttac gctgcggctc
    14401 taactccgca aaggcatcta gtctggcatg ctgaccgacg gcgagaataa ttttatctgc
    14461 cgccatcgtc agttcgcccg ataaccgtac atgcttaaac gtgactttat tgccttccac
    14521 ggctactggc gtgaatccat cgatgatcga aacgcccagc tcccgggcgc tggtaaactc
    14581 tttttcgctt gccggaaact catctaactc ttcacgcgct acgcaagtta ccgcctgaca
    14641 gccgagaact ttcagcgtgc tggctacgtc catcgcgaca tcaccgccgc cgataattaa
    14701 tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt gcacgttgca agaagtcgac
    14761 ggcaatctca acgtcactat gctcaaacag cggtagcccg gaaccgcttg ataaccccac
    14821 ggtgaccagt accgcgcggt tttctgcttt cagctgctca agggtgagtg tgttaccgac
    14881 ttcgttgttg cacttaatgg tcacgcccat tttttcaata cgggcgatct ctgcatccag
    14941 tactgattgc ggtaaccgga attgcggaat gccgttacgc aatcagccac cgggttgtgc
    15001 ttctttctca tagatcgtga cgtcataacc ctggtttgtc agtgtcacac tggcctgcaa
    15061 tccggcagga cctgcgccaa taatcgcgac tttgccgagc gttttagtac cgggctgata
    15121 aatttccatc ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc ggccgatatc
    15181 aatgggagca tcaacaccgg cacgggtaca accgctttgg cataatttct ccgtcgggca
    15241 cactctggca caaacggcac cgagggcatt attttcgcga attgtctcgg cagcgccttt
    15301 aaaattacga aagtagattg agcgaataaa tttccccgga tcggtctgtg ccgggcaagc
    15361 ctgactacag ggagcgtcat gacataataa acagcgagag gcttctttaa tcgccagtaa
    15421 aggcgtaaaa gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt
    15481 aatgctatga gtaattaagg cgaacgctaa ataaatattg cgtgctgata ttaaggctaa
    15541 gcgtgtgggg aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat
    15601 atccgcagct tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat
    15661 attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg aagccgatat
    15721 atgccaccat ataagcgaaa aagagtccgg gattttttgc tcgccagaca tacaccggtt
    15781 ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc
    15841 ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca
    15901 taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga cggcgggata
    15961 cccctgcgca tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa
    16021 acgcggttca cgtctaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc
    16081 gcgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat
    16141 ccccatatgc agcgcaataa ataatgcccg ctcacagtgg aaacgttggg tgataataat
    16201 gaaatcatta gtatcgaaca ctttgcgtgt acgcacgatg gaatccagcg tacgaaagcc
    16261 tgcgtaatcg agaacaatat ctgatgggtc gacaccagca gcgattaaat ctttgcgcat
    16321 ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata gataatttac
    16381 cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat
    16441 tacgccagta cgataatatt ttgctgtgcc gagcaccacg ccgacctggc ggtaggggag
    16501 atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc
    16561 gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca cgcgctttaa
    16621 catgcaggag actcgatcta aggaaaattt ccatcaggct actttacccg ctggttttgc
    16681 gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca ggagagggct ggcaagccgg
    16741 acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagcacgcg gtcgatactg
    16801 tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg
    16861 gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaac
    16921 agaattaacg ccccctgcaa cggtggcaga gtcgggttga atgcggcgtt ttcagcgtag
    16981 ctgccgctga aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg
    17041 taaggcatgt gcgaacggtt tgccgccgca atcgctgcct gagaaagcgc atcacccgtc
    17101 agcgcatagc cgtgatcctg ttcgtccatc agcagcgttt taatctccag atctttcggc
    17161 ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct cgcggcccgg cagatgaata
    17221 cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgta
    17281 tagttaacgg tgatggctgc aagcgctttt tcaccactca accaggcgtg gctgatcgcg
    17341 ctttgttcgg catgaacggt ttgctgcatt gttgcaccaa taaattccat attggcaccg
    17401 aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc
    17461 gtacgcgcac aggccgccgc cagtggaagt agtgcgaatg ccagcgcgtc ttcgtccagc
    17521 cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg
    17581 tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt
    17641 tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac
    17701 ccgttatggc tttatatgtg atctgaatct cattaattat gcaacgcgtg aaacttacat
    17761 caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat cgggaaaagg
    17821 aacggtgcga tcagcgaagt aattatcccg cataacacca gcgccagcga actaaatgca
    17881 ccttcctgat aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga ggctgtcccc
    17941 attgccagac cgcgcgcagc tttggtacga atacgcatcg cattaagcaa tgtatggcca
    18001 aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc cggaataccg
    18061 ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac
    18121 gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca
    18181 accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt
    18241 agctgctcat ataaaggata ggccagcgcg acgaccgccg gttgcagcag atcgtttagc
    18301 acttcgctac ctttaaagta gctgtcgtaa gagatgccgg tcagcattaa aaaaggaatg
    18361 atcaccacca ttgctaccag caacgggtta agcaggggaa acttataccg tgccgccagt
    18421 ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg accaccagat attcgccatc
    18481 attcttctga tcctttctga cctacgactt tacgttcacc gtgtaccagt tgtgaactcc
    18541 agctcaccac cagaaaaacc accagcgtac tgactgcaca ggaaaccact accgggccaa
    18601 actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata
    18661 gcgccatata gcgaatcagt acgtagcacc ccggattgac ccattttgcc ggaagaattt
    18721 gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg
    18781 gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acgaaagcgc
    18841 gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg
    18901 attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa tcagcgctta
    18961 ttttttcgct ccccggcatt acgcaatgcg caacgaactc aggcatacat caagcataga
    19021 gagggattac agtttgcagc aatccctctt tgcagacctt taatccgttg accataggcg
    19081 ctaacatagc gcctcatttt tttccgggtg aagatatgcg tgtgttactg gctccgatgg
    19141 agggtgtgct tgactctctg gtgcgagaat tgctgaccga agttaacgac tacgatctgt
    19201 gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc tttcatcgca
    19261 tttgccctga gctacaaaac gccagccgga caccatccgg tacgctggtg cgcgttcagt
    19321 tgttaggtca gttcccacaa tggctggcag agaacgccgc ccgtgcggtg gaattaggtt
    19381 cctggggcgt ggatctcaat tgcggctgcc cgtcgaaagc ggttaacggc agcggcggcg
    19441 gggcgacgtt actcaaagat cctgaactca tctaccaggg tgcaaaagcg atgcgtgaag
    19501 ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct gggctgggac agcggtgaga
    19561 agaaatttga aatcgccgat gcggttcaac aggctggtgc tacggagctg gtggtgcatg
    19621 gacggacaaa agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga
    19681 ttcgccagcg gctgaatatt ccggtgattg ccaacggcga aatctgggac tggcagagcg
    19741 cgcaacaatg cattgcgatc agcggctgcg acgcagtgat gattggtcgc ggggcgctca
    19801 atattcccaa cctgagccgg gtagtaaaat ataacgaacc gcgaatgccg tggccggagg
    19861 tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc
    19921 acgttgcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacgg
    19981 aattatttca gcatgttcgg gtgttgaata attcccctga tattgcaagg gctattcagg
    20041 caattgatat cgagaaactc taaaaaacca tagtgatgtt ttttgacaat gtgttagctc
    20101 tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat tgccgaggcg ttccctgaag
    20161 gccggggcag cccacatgga tgt
//
LOCUS       DAZPJP010000063        20139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPJP010000063 DAZPJP010000000
VERSION     DAZPJP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.063"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            225..725
                     /gene="tssB"
                     /locus_tag="ACHU9J_002072"
     CDS             225..725
                     /gene="tssB"
                     /locus_tag="ACHU9J_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR1905609.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            760..984
                     /locus_tag="ACHU9J_002073"
     CDS             760..984
                     /locus_tag="ACHU9J_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905610.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1035..2510
                     /gene="tssC"
                     /locus_tag="ACHU9J_002074"
                     /pseudo
     CDS             1035..2510
                     /gene="tssC"
                     /locus_tag="ACHU9J_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2517..2930
                     /gene="tssE"
                     /locus_tag="ACHU9J_002075"
     CDS             2517..2930
                     /gene="tssE"
                     /locus_tag="ACHU9J_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1905611.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2934..4784
                     /gene="tssF"
                     /locus_tag="ACHU9J_002076"
     CDS             2934..4784
                     /gene="tssF"
                     /locus_tag="ACHU9J_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1905612.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4748..5830
                     /gene="tssG"
                     /locus_tag="ACHU9J_002077"
     CDS             4748..5830
                     /gene="tssG"
                     /locus_tag="ACHU9J_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR1905613.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5855..7135
                     /gene="tagH"
                     /locus_tag="ACHU9J_002078"
     CDS             5855..7135
                     /gene="tagH"
                     /locus_tag="ACHU9J_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR1905614.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7132..7656
                     /gene="tssJ"
                     /locus_tag="ACHU9J_002079"
     CDS             7132..7656
                     /gene="tssJ"
                     /locus_tag="ACHU9J_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR1905615.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7659..8990
                     /gene="tssK"
                     /locus_tag="ACHU9J_002080"
     CDS             7659..8990
                     /gene="tssK"
                     /locus_tag="ACHU9J_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR1905616.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8995..9756
                     /gene="icmH"
                     /locus_tag="ACHU9J_002081"
     CDS             8995..9756
                     /gene="icmH"
                     /locus_tag="ACHU9J_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR1905617.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9765..12578
                     /gene="tssH"
                     /locus_tag="ACHU9J_002082"
                     /gene_synonym="clpV"
     CDS             9765..12578
                     /gene="tssH"
                     /locus_tag="ACHU9J_002082"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR1905618.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESEQDNTGAEPADEADREQPEETAE
                     TVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVIT
                     DLTKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQL
                     AELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLL
                     DEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPET
                     MQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVV
                     IEPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVD
                     GAFTADVEDAQNDESVTKDETVL"
     gene            12575..13318
                     /gene="vasI"
                     /locus_tag="ACHU9J_002083"
     CDS             12575..13318
                     /gene="vasI"
                     /locus_tag="ACHU9J_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR1905619.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13323..14735
                     /gene="tssA"
                     /locus_tag="ACHU9J_002084"
     CDS             13323..14735
                     /gene="tssA"
                     /locus_tag="ACHU9J_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR1905620.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14844..18278
                     /gene="tssM"
                     /locus_tag="ACHU9J_002085"
                     /pseudo
     CDS             14844..18278
                     /gene="tssM"
                     /locus_tag="ACHU9J_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18289..19738
                     /locus_tag="ACHU9J_002086"
                     /pseudo
     CDS             18289..19738
                     /locus_tag="ACHU9J_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19665..>20139
                     /locus_tag="ACHU9J_002087"
     CDS             19665..>20139
                     /locus_tag="ACHU9J_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1905621.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDR"
BASE COUNT         4742 a         5517 c         5581 g         4299 t
ORIGIN      
        1 gcgaattgca caggttcaca caatctattt aacgcttgca gaatacccct ctttacgatc
       61 gataaaaagt ccttttctta tttattttga agcgtaaaat gcggggtgat gaaggagtgg
      121 tgattttatt ttcaccgcca ttagggcggc attgccgata tttttttgcg gtacaaatat
      181 gccgtgttca tggcctgtac gagataacat gacggatcac ttttatgagc aaaaaatttg
      241 aaggcagcgt cgcgccccgc gagcgtatta atatatcgta tgtcccaaaa acagatggcc
      301 agacggctga agtggaactt ccgcttaaca tgctcgttgt cggcgatacc ggaaatacgc
      361 aggaaacatc cccactggat gaacgtcagg cagtttccgt taacaagcac aatttcggtg
      421 cagtgatggc tgaagcagcc attggcctca attttactgt ccccgccacg ctgaagggaa
      481 gcacaacaga cgatgaactg aatgtggcgc tgaatatcaa atcactggat gatttttcac
      541 cagatagcgt ggcccggcag gttccggaag tgaataaatt gctggaactg cgtgaagcac
      601 tgactgcact gaaggggccg atgggtaacc ttccggcatt ccgtacccag ctccaggcat
      661 tgctggaaaa tgaagagtcc cgcgagcagt tgctcaaaga aatcggtctg gtgagcaata
      721 aataaccacc actatttcat taaacagggt tatccaggca tgacggcaca caaaaatatc
      781 tccgggacat accacctctc tgttgcagac attctgcagg tggtttacca ggtatgtttc
      841 agcccgtccg ttgaaatcaa ccaggatggt gtggctgcgc tgattacaac actggatcgc
      901 cgtatcagtg atctattgga tgagattatc catttttgtg agtttcagca gagcgcgtcg
      961 cactggcagc gcgtacttca ctgatggcga aaagccggtt tcagaaacac agctaaaaca
     1021 gaaggatttt cagtatgtct ttacaggaag aggaactcgt ttccagtcat gccgggcagc
     1081 ctgagcaggc atcgtcgctg cttgatcaaa tcatggcaca gacccgtatt cagcccggtt
     1141 cggaaggtta tgacgttgcc ctccagggtg tgacagcctt tattgccagc atcctgcaat
     1201 ccactgcatc tgccgaaccg gtcaataaac tggccgtgga cagcatgatt gcagatattg
     1261 atgaacgcat cagccgccag atggatgtca tcatccatgc gcctgccttt cagcaggtcg
     1321 agtcgttctg gcgctcactg aaaaccatgg tggatcgcgt tgatttccgt gaaaacatca
     1381 aggtcaacgt gcttcatgtc accaagcagg aacttctgga agactttgag tttgcgccag
     1441 aaattattca gtccggattt tataagcacg tttattcgtc cggtttcgga cagtttggcg
     1501 gtaaacccat cgctgccgtg ctgggcgctt atgagttcaa aaataccgcg cctgacatga
     1561 aactgctgca atacgtcagt gccgtgggcg caatggcgca cgcaccgttc ctgtcctccg
     1621 tttccccgga gtttatgggg ctgaactcgt ggaccgaact gcctaatatc aaagatcttt
     1681 atgccatctt tgaaggtccg gcctacacca aatggcgcgc cctgcgtgac tcggaagatt
     1741 cgcgctattt agggcttacg gcaccccgct tcctgcttcg ccagccctat tcaccaacag
     1801 ataaccctgt taagaacttt aactattacg aagatgtcag ccagaaccac gaagattatc
     1861 tgtggggtaa tacggcctgg atgctggcat gcaatatcgc agacagtttt gccaaatacc
     1921 gctgatgtcc aaacattatc ggtccgcaaa gcggcggcgc agtgaaagat ctgccgatgc
     1981 atctgttcga aacgatgggg cagattcagg ccaaaatccc aaccgaggtg ctggtcaccg
     2041 atcgccgcga atttgaactg gctgaagagg gttttatcac ccttaccatg cgtaaagact
     2101 ctgataatgc agcctttttc tctgcaaact cggtacaaaa accgaagcac tttccgggaa
     2161 aagatgcaga aaccaactat aagctgggta cgcagcttcc ttatctcttc atcatcaatc
     2221 ggttagcgca ctacatcaag gtgttgcagc gtgaacaact ggggtcatgg aaagaaagga
     2281 gcgacttaga aagagagctt aatacctgga tccgtcagta tgttgccgac caggaaaacc
     2341 cgcctgcaga cgtgcgcagc cgcaaacctc tgcgcgctgc caaagttgag gttatggatg
     2401 tagaaggcga acccggctgg taccaggttg cgctaagcgt gagacctcat tttaaattta
     2461 tgggggcaaa ttttgagctt tccctggttg gccggttaga cagggagtaa cacaccatga
     2521 taccggatcg ggaaagaacc acaggttctc tttttgagcg tatggaagcc tcttcggcca
     2581 gaaaccggca gggaggcagt attcattcgc tcaggcaatc cattcgccag aacctacgaa
     2641 acattcttaa tacccggtcc ggtagttgcc ggggtgcgcc tgagctgggc attgatgaac
     2701 cggaaggggc tgaaaacttc cgcgaatcca tgagtcgggc catcgagcaa tgtatagaac
     2761 gctatgaacc ccggatctca cacgctgaag tgcaggcagt ggtttcatcc gcctccagcc
     2821 cgctggatat gacgtttcat attacagcct gggtgacatt taacgaaacg catgaggtgc
     2881 ttgaatttga tatggcaccg aatggcagcc agcattaccg ggtggactga caaatggaat
     2941 ttgaagaacg ctatttccgg gaagaactcg attacctgcg ccagcttagc aagctgctgg
     3001 caacggaaaa accccatctg gcccgcttcc tggccgaaaa agatgcagat ccggatattg
     3061 aacgcctgct ggaaggggtg gcttttctta ccggcaatct ccgccagaaa attgaggatg
     3121 aattcccaga actgacgcac gggcttatta agatgctatg gcctaattac ctgcgtccgg
     3181 ttccggcaat gacccttatt gaatatacgc cggatatgga taagtcttct gtaccggtgt
     3241 taatcccccg taatgagcag tttacaacca acgccgggga aatcagagtt gatgaagtgc
     3301 tgccctctga tgctaaaaag gaggagccgc ctccctgtac cttcaccctc tgccgggata
     3361 tctggctgct gcccgttcgc ctggagcaga ttgaaaaccg cagtacgacc cgtaatggtg
     3421 ttatcaacat caccttttcg gtcgcaccgg gaacggactt ccgcacgctg gatctgaaca
     3481 aacttcgctt ctggctcggc aatgacgaca actatacccg tgaccagctt tatttatggt
     3541 tctgcgaata cttgcagggt gccgacctga ctgtgggtga acagcatatt cgcctgcctg
     3601 agtttatgct aaaagctgtc ggttttgagc cgcaggatgc catgctgccc tggccgaaaa
     3661 acgtccacag cggctaccgg atccttcagg agtatttctg ttaccccgat gcgtttctct
     3721 tttttgatct ttgtggttgt ccggctttgc ctgacggatt gcaggcggaa ttctttaccc
     3781 tgcaactgcg tttttcgcgc cctttgcccg tggacatccg gctgcgccgc gattccctgc
     3841 gcctgtattg cgcacctgcc attaatttat ttatccacca tgcagaagcc atcacgctgg
     3901 acaaccggcg ggcagactat ccgctggttc ccagccgcca ttacccacaa cattacgatg
     3961 tattttccgt taacagtgtg gtgagccagg tccaggatat gttcaggaaa aaagatctgg
     4021 ggcgtcctgt ttcgacgcag gccgcgcgcc agtggccagc ctttgaaagt ttcagccatc
     4081 agatagaata cagccggaag cgggaagtgg tgtactggca tcaccggacc aaaacatccc
     4141 tgttccatcg cggctttgat catacccttg cctttataca tgctgatggc agttatccgt
     4201 cagacgaatc tctgctcagt aatgaagtgg tttcggtatc gctgacctgt accaaccgtg
     4261 agcttccgtc acaaattcgt tccggcgata tcaccggcac aaccggaaaa aatgcggctg
     4321 ttgcttcatt tcgcaacatt acccgcccga cgcaaccact ctggccggtc attgatggca
     4381 gcctgcactg gcccctactc tccgccatga acctgaatta tctgtcatta ctggatacgg
     4441 acgcgctgaa gcaggtcatc gccaactttg atcgccacgc aatccatcat ccgcagacgg
     4501 cacggctgtc acaacaaaag ctggatgcca ttgagcgtct ggagacccgc cccgttgatc
     4561 gcctgtttac gggtattccc gtccgggggc tggcttccac gctgtatctg cacccggagc
     4621 cgtttgtctg tgaaggggaa atgtatctgc tcggtacggt gctttcgcat tttctgtcgc
     4681 tgtacgccag cgttaactca ttccacatgc tgaccgttgt gaacacagaa agccaggaga
     4741 catggaaatg gacggaaaga atcgggcagc atcctcttat ctgagtccgg gaaatccccc
     4801 ggcagataaa gagcagaatg atccgctggc acaggttttc cataatgcct gctcttacaa
     4861 tttttttgcg atggcggagc tgttgcaccg cctggcaaag ggtgaaaagg ggacgccaga
     4921 attatccctg cgcgacgatc cggcacagga aaccctgcgt ttcagcgccg atgccagcct
     4981 tgccttcccg tgcagtgata tcagtgcgct gaaacgggat acgtcagggg cattcaggat
     5041 gaccaccacc tttatggggt tacaggggag ccagtcgcct ctccccggtt attacctcgc
     5101 tcacctggcc tggaaagcgg tacatgaaca aagtccggta ggtgattttc tggatatgtt
     5161 cagccaccgc ctgacccagt ttgtctggca tatctggcgt aagtaccgct atcacatcag
     5221 ttttcgtaac ggcggtgtgg atgcgttctc gcagcgcatg tactcccttg tcgggctggg
     5281 ccaccgccag cttcgcgata agctggcgat taatcacagc aaaatgctgg cgtattccgg
     5341 gatcctggca aatccgggac gatccccgga aattatctgt ggcctggtgt cccactgctt
     5401 tgatttgtca gaagtgacac tgcaaaactg gcaacgtcgc aaggtggata ttgaaccgga
     5461 tcagcaaaat agtctgggga gctattctct taaaaatggc gaaaaactgg cgggaagatc
     5521 ggtactgggc aactttgttc tgggtacacg agtgcccgat ctcagcggaa aattccagct
     5581 cagtattacc agcctgaccc gaaaacagtt cctctctttt ctgccgtccg gcgaaaactt
     5641 cctgccactg acgatgttcg tgtccttcat cctgcgcgat cagcttgcct gggacttaca
     5701 tctggggctg gcgccggagc aggtgggcgc aatgcgcctg ggcgataaca aaagtgcgct
     5761 gctgggctgg accagcttcc tcggcacacc ggaagaacga ccctcagtca ccatcagggt
     5821 acggtcgtaa attatgataa tcacggagca gaatatgccg gaagagaaac tacagacgct
     5881 ttcattgcag gtcattaacg gcagtgagct ggaaagcggg cgggcggcgc gctgtctgtt
     5941 cacacagcag ggaaatgtcg gccatggccc cgaatgccac tggtcggtac aggatcgtca
     6001 gcagagcatt ccggcccagg cttttaccgt tatcctgcac gatggcacat tttgtctgcg
     6061 cccgcagacg gcacaactgt ggctgaatca ggcaaaagtc acagcaacat cagacctaat
     6121 acagttgcgc cagggcgatg agatccagat cggacggctg atggtgaggg ttcatctgaa
     6181 ccggggagat attccccatt acgatgagga aatggccact cccgaaacca tcgttaccaa
     6241 tcgcgatatg ctcacggata ccctgctatc aacggagggt gcgccacact atccgggaat
     6301 gactcaccgg caccagcttg cagacaccgt ggtaaacggt ttttctgccg atccactcca
     6361 ggctcttcag tccgaaagcc tgattaccac gggcgatccg ctttcaggca ttgcggctgt
     6421 ccggccatcg gcaccgctgt ccgatccggc aagtaatggg gggatcaata ctccgtttat
     6481 ggatctgccg cccatttatg ccagccccgg cgatcgtaat gacgacgtct ctgcggcaga
     6541 aatggcgcaa cgccacgttg cggtcacccc cttactgcgc ggtcttggcg gctcgcttac
     6601 catgagcaat tccgacgatg cggatgattt tctggaggag gccggacgaa cgttacaggc
     6661 cgcaataaaa ggtctgctcg atttgcagca gcagcgtaac agcctctcag acaaacattt
     6721 gcgcccgctg gaagataacc cgctgcgcct gaacatggat tacgccaccg cgctcgacgt
     6781 aatgtttgcc gaaggtaaaa gcccggtaca tctggcggct cccgccgccg tcagtgaaag
     6841 cctgcgcaat gtccgccacc acgaagaagc taaccgggca gcgattgtgg agtcgcttcg
     6901 tgtcctgctg gatgctttct caccacaaaa tctgctgcgc cgctttgtgc agtaccgccg
     6961 cagccatgaa ctgcgccagc cgctggatga tgccggagca tggcaaatgt acagccatta
     7021 ttacgaagaa ctggcctccg atcgccagca ggggtttgag atgctgttta acgaggtcta
     7081 cgcccaggtc tatgaccggg tgcttcgtga aaaacagcgg gagccggaag catgaacagg
     7141 aaggcgtttc tggcctgcgt gctgatgtgc gtcatattaa ccggctgcga aacggcgaaa
     7201 aaaatcagtg aggtgatcaa aaacccggat atccaggtcg ggagcctgaa ggagcaacct
     7261 tcggaaataa ccgtcaccct gctgaccgaa ccggacacca ataccaatgc ggaaggtgaa
     7321 tcagccgccg tggatgtgca actggtgtac ctgactgacg actcaaaatt gctggcagcc
     7381 gactatgacc agatagccag cacgccgcta ccggatgtgc tgggtaaaaa ctatatcgac
     7441 caccaggatt tcaacctgtt gccggacacc atcaaaaccc tgccgccggt aaagctggat
     7501 gagaaaacgc agtttattgg tgtcgtggcc tatttctccg atgaccaggc cactgaatgg
     7561 aaacaaatcg agacggtgga aggaatcggc caccattacc gcctgctggt gcatgtacgt
     7621 caaagcagca tcgaaatgaa aaaagaggat gaataattat ggcaaccacc cgcaacaagg
     7681 tgatgtggca ggaagggatg ctgatgcgtc cacaccattt ccagcagcag cagcgttaca
     7741 acgactacct ggataaccag cgtttccggg ccatgaatga tttatcctgg ggatttaccg
     7801 aactcaccct caacaatgaa ctgctggcgc agggtaagat catgattgac agcgcgtcag
     7861 gcacactgcc cgacggcacc gtcttttcta tccccgacca ggacgcactg cccgatccgc
     7921 tgcacccgca aaattttccg gacgaaagaa gccgcaatat ctacctcgct ctgccggtcg
     7981 ccagtgatgt gagaaatgaa atcagcgacg gacggcgaat cgggcgttac cggctgaatt
     8041 atgccgatgt ccgggatttg cattcagaag aaggtgacgc gcgaacattg acgctgggtc
     8101 aactgacgcc gcgcattatg agcggtgcag aagatatgag cgcctatatt acgctcccgc
     8161 tttgccgtat cagcgatcgc catgccgatg gctccctgac gttggatgac gattttatcc
     8221 cctcctgcca gaatattcag gtcagtaaga aactgcgtgt ttatctcaaa gaggtacagg
     8281 gggccattgg cggacgggca agcgatctgg caaaccgtat tggctctccg gcgcagagcg
     8341 gcatcgcgga tgtggcggaa tttatgatgt tgcagttact taaccgtaac cagacccggt
     8401 ttacccatca cgcccgtcga tcccagctcc acccggaaga tttctacctt gatcttgccg
     8461 ggttgctggg tgaactgatg acctttaccg agccgtcgcg cctgccctgc ccgcttgatg
     8521 tgtatgatca tcatgacctg accaaaatat ttaaaacgct gttaccggaa gtcaaacggg
     8581 cgctgcatac cgtactgtcg ccaagagccg tcaatctgcc gctgcatctg cgtgacggta
     8641 tctggcaggc cgatatccat gacacagaac tgctgcaatc tgccaccttt gtgctggccg
     8701 tggcggcaaa tatgccggtc gatcagatcc agcgccagtt tatccagcag tcgaaaattt
     8761 cctcgccgga aaaaatccgc aatatggtca gtgtgcagat cccctgtatt ccattgcgtg
     8821 cccttatggt ggctccccgc cagcttcctt accattccgg gttcagctat ttcgaactcg
     8881 acaagagtgg ccaggcctgg acagaaatgg ctgccgccgg ggccgttgca ctgcatgttt
     8941 ccggcagttt cccggatctg aacatgcaac tgtgggcgat aagagggtaa gacgatggat
     9001 gaggggtcac tgtctctgcc accatttacc ggacacgacg aaaaaagcca gcgcaactac
     9061 catctcgcgc ttcgtggcaa cagcctgaat ccgatgatcg acgcggccac gccgctactc
     9121 ggtatggtaa tgcgcctttc aaccatgaac agccagacga tgccggagca tctttttgct
     9181 caggtggtga cagatgttca ggccgtggag caactgcttc aggagcaggg ctacgaaccg
     9241 ggcgtgatta tctcgtttcg ctacatcctc tgcacgttta ttgatgaagc ggcgctcggt
     9301 aacggctggt caaacaagaa cgagtggatc aagcagtcat tgctggtcca tttccacaac
     9361 gaagcctggg gtggcgagaa agttttcatc ctgctggagc gcctgatccg tgaacccaaa
     9421 cgttaccagg atctgctgga atttctctgg atctgttttt ctctgggatt tcgcgggcgc
     9481 tacaaagtgg cagcacagga tcagggggaa tttgagcaga tctaccgccg tctgtaccac
     9541 gttctgcata aactgcgcgg cgatgcccct ttcccgctgt tacaccagga caaaaaaacc
     9601 cagggcgggc gttatcagct catcagtcgc ctgactgtta aacatatttt ttgtggtggc
     9661 gttgtcgtgc tggcgttgtt ttacctgttc tatctgttgc ggctggacag ccagacgcag
     9721 gacatcctgc accagctaaa caaattactt cgataaggac gtttatgatc cagattgatc
     9781 ttcccacgct ggtaaaacgg ctgaacctgt tctcccgcca ggcgctggag atggccgcct
     9841 ctgaatgtat gagtcagcag gcagcggaaa ttaccgtcag ccatgtgctc attcagatgc
     9901 tcgccatgcc acgcagtgac ctgcgggtta ttacccgcca gggcgatatt ggcatggaag
     9961 agttgcgcca ggcgctgacg gtagagaact acacaaccgc ccgttctgcg gacagctacc
    10021 cggcgttttc cccgatgctg gttgagtggc ttaaagaggg ctggctgctg gcgtcggctg
    10081 agatgcagca cagcgaactg cgcggcggcg tgttgctgct ggccctgctg cattcgccgc
    10141 tgcgttatat accgcctgct gccgcccggc tgttgaccgg cattaaccgt gaccgtctgc
    10201 aacaggactt tgtgcagtgg acacaggagt cggcggaatc agtcgtgccg gatgcagacg
    10261 gtaaaggcgc aggcacaatg acggacgcct ctgacaccct gcttgcccgc tatgccaaaa
    10321 acatgaccgc agacgcccgt aacggcaggc ttgacccggt actgtgccgc gaccacgaaa
    10381 tcgacctgat gatcgacatt ctctgccgcc gccgtaaaaa caacccggtg gtggtgggcg
    10441 aagcgggcgt gggcaaaagc gcactgattg aagggctggc gctgcgcatc gtggcaggcc
    10501 aggtgccgga caagctgaaa aacaccgata tcatgaccct tgacttgggc gcattgcagg
    10561 ccggggcgtc ggtgaagggt gaattcgaaa aacgtttcaa agggctgatg gcggaggtca
    10621 tttcctcccc ggtgccggtc attctgttta tcgacgaagc acataccctg attggcgcgg
    10681 gcaaccagca gggcgggctg gatatctcca acctgctcaa accggcgctg gcgcgcggcg
    10741 agctgaaaac catcgccgcc accacctgga gcgagtacaa aaaatacttc gaaaaagatg
    10801 ccgccctgtc gcgccgcttc cagttggtga aggtcagcga acccaacgct gccgaagcca
    10861 ccattattct gcgcggtctg tcggcggtct atgaacagtc tcacggcgtg ctgattgatg
    10921 atgacgcctt gcaggccgct gcgacattaa gcgagcgtta tctctccggg cgtcagttac
    10981 cggacaaagc gattgatgtg ctggataccg cctgcgcccg tgtggccatc aacctgtcgt
    11041 cgccgccgaa gcaaatctcg gcgctgacca ctctgagcca ccagcaggag gcggaaattc
    11101 gccagcttga gcgcgagctt cgcatcggac tgcgtaccga cacatcacgg atgaccgagg
    11161 tgctggtgca gtatgatgaa acgctgacgg cgctggatga actggaagcg gcctggcacc
    11221 agcagcagac gctggtccgg gagattatcg cgctgcgcca gcagttactg ggcgtggcag
    11281 aggacgatgc ggcgccgttg ccggacgcag ataccgtgga ggatacgcag ccagagtcag
    11341 agtcagagtc agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagcagg
    11401 ataataccgg tgccgaaccg gctgatgagg ccgacagaga acaaccggaa gagaccgctg
    11461 aaacagtttc cccggtacag cggctggccc agctcactgc cgaactggac gccctgcata
    11521 acgaccggtt gctggtctcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg
    11581 aatggaccgg cgtgccgctc aaccgcctgt cgcagaatga aatgtcggtc atcaccgacc
    11641 tgacgaaatg gctgggtgac accatcaaag gccaggacct ggcgattgcc agcctgcata
    11701 agcatctact gaccgcacgc gccgacctgc gtcgtccggg acgcccgctc ggtgcgttcc
    11761 tgctggctgg ccccagcggt gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc
    11821 tctacggcgg tcgccagtac ctgaccacca tcaatatgtc cgaattccag gagaaacaca
    11881 ccgtctcgcg gctgattggt tcgcctccgg gctacgttgg ctacggtgaa ggcggcgtac
    11941 tgaccgaagc gattcgccag aaaccctact cggtagtact gctcgatgaa gtggaaaaag
    12001 cgcacccgga tgtgctcaac ctgttctacc aggcgttcga taagggcgaa atggcagacg
    12061 gtgaaggccg cctgattgac tgcaaaaata tcgtcttctt cctgacgtcc aacctcggct
    12121 accaggtaat agtcgagcat gccgatgacc cggaaaccat gcaggaagta ctgtatccgg
    12181 tgctggccga cttcttcaaa cctgccctgc tggcgcgtat ggaagtggtg ccgtatctgc
    12241 cgctgtcgaa agagacgctc gccaccatta tcgccggaaa actggcccgt ctggataacg
    12301 tgctgcgcag tcgctttggt gtagaagtgg tcattgaacc ggaagtgacg gacgaaatca
    12361 tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtc atcgatggcg
    12421 acatgctacc gccgctctcg ctgctgctgt tgcagaaaat ggcggctaac acggcgattg
    12481 cccggattcg gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga
    12541 acgacgagtc cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca
    12601 gacaggatgg tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga
    12661 agcatcgtca atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac
    12721 agccacgtta caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga
    12781 ctgctatgac cgcctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa
    12841 agccggtttt gtgggcgagg cctggacccg cgccacagag caggaaaaac gccgcgaggg
    12901 caacaccaca gaactgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac
    12961 caccccggct atcggtcatg ttccgccacg cccggtgctg atgttcagtt gcgtggacaa
    13021 tatcacccgg atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac
    13081 tctgaatgcc gacaaccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct
    13141 gctggagtcc agccgggggt tatccgggat tgatgaaatt aagcaactgt tcggcgcaaa
    13201 aacgctgacg gtggataccg gcgcagacaa tgccgccgga aagctgacct ttaacattga
    13261 cgggctggca agggcgattg ctcccctgcg cgatgcctgc cactgggcgg gagaataaaa
    13321 atatggcaat ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc
    13381 cggaagagaa actggcatcg gcgctgaaag atgacaaccc gaactgggag tatatcgacg
    13441 gcgaaatcgt caagctggga tcgctggccc acagtcaact ggatatcccg gaactccagc
    13501 gccgggggct ggtcattctg gcttcggaga gtaaggattt tcgtctgctg gcgcatctgc
    13561 taagaaccct gcaacacgcc ggtgatcctc atctcgcatt gcgcctgctg gcgctgtatg
    13621 tggagcatta ctgggcggtg gctgcgccgc agaatatggc gcataaaaaa cgctttgcca
    13681 gccaggtcat taaacgcttt gaaacaggta ttgaagtttt ttcacagaac gccgccacag
    13741 cgcagcgcga tgcgctgtca gggcaactgg caaaactggc gcaatgctgg cagtcacata
    13801 acgccccgga actggcacag gctactgatg atctttttgc cctgtaccag cgtgcgttta
    13861 atcgtgcggc accggctcct gtccccactc cggcggcctc cggtagttca ccacagacca
    13921 ccgccacgtc tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgtcatcg
    13981 acagtcacga cgacaaagcc tggcgcgaca cgctgttaaa agtggcggcc attttatgtg
    14041 aacgccagcc ggattcaccg cagggctacc gtctgcgccg ccatgcgctt tggcagaaca
    14101 tcaccagtac gccccaggcg gaaagcgatg gccgtactcc actggctgcg gtctccgccg
    14161 atatggtggc agattaccat gctcagctcg gtagtgccga tatggcgctg tggcagcagg
    14221 tggagaaaag cgtactgctg gctccttact ggctggatgg tcactgcctt tctgcacaga
    14281 cggcgctgcg tctgggttac aaacaggtgg cagacgccat ccgcgatgag gtcatccgct
    14341 tccttgagcg tctgccccag cttaccgggc tgctgtttaa tgaccacaca ccgtttatca
    14401 gtgagcagac gaaacaatgg ctggcagctt cgcccgacgc caaagttgca ccggttgcgc
    14461 aaatcggtga ggaatcgaag gcagccagag cctgttttgc tgagcagggt ctggaggcgg
    14521 cgctgcgata tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt
    14581 accttgccgc acagttgacg gaagaggcgg ggctggtaca gcttgcgcag cagcagtacc
    14641 ggatgttgtt caggatggga ttgcagatga tggtggcaga ctgggagccg tccctgcttg
    14701 agcagctaga acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca
    14761 gattaccaac accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc
    14821 ggtttaaagt ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga
    14881 tctggtggaa aggcccgatg tggacgctgt atgaagaaca gtggctcaaa ccactggcga
    14941 accgctggct ggcaacggcg gcgtggggga ttattgccct ggtgtggctt accgtccggg
    15001 tgatgaagcg cctgcaacag cttgaaaaaa tgcaaaagca acagcgcgag gaagccgttg
    15061 atccgctcag cgtggaactg aacgcccagc agcgttatct tgaccgctgg ctgctgcgcc
    15121 tgcaacgcca tctcgacaac cgccgtttcc tgtggcagtt gccgtggtat atggtcatcg
    15181 gcccggcggg cagtggcaaa acgacactgc tgcgcaaggg gtttccgtcc gacattattt
    15241 atgccccgga gggcgcacgc ggcgcagaac aacgcctgta cctcacgccc catgtcggta
    15301 aacaggcggt gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc
    15361 tgcatcgccg tctgtgggaa catgccctcg gctggctgaa agaaaagcgc gcgcgccagc
    15421 cgctgaacgg gattattctg acactcgatt tacccgatct gctgaccgca gacaaacgcc
    15481 gccgcgagca tctgttacag acgctgcgca gccgcttaca ggatatacgc cagcatcttc
    15541 actgccagtt accggtttac gtggtactga cccggcttga tttattacag ggttttgccg
    15601 ccctgttcca gtcactcaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc
    15661 gccgtgccca tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg
    15721 tggatcgaat gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt
    15781 ctttattcag tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg
    15841 agggtctgct tgatggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt
    15901 cgcttcagcg tggacagatg gatgatatat tcacccagtc tgccgcccgc cagtaccggc
    15961 tgggcaataa cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca
    16021 gcctgttccc gcaggcatta ctcgcagagc ctaatctggc aacagagagc cgcgcctggc
    16081 tgatacgttc ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcagcactgc
    16141 tgctcatcac cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc
    16201 ttaagcaggc caaagccttt atggacgtgc cgcctccgca gggggaagat gactttggca
    16261 acctgcaact gccgctcctg aacccggtac gcgatgccac actggcctat ggcgactggg
    16321 gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg
    16381 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
    16441 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
    16501 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
    16561 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
    16621 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
    16681 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
    16741 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
    16801 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
    16861 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
    16921 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
    16981 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
    17041 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
    17101 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
    17161 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
    17221 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
    17281 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
    17341 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
    17401 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
    17461 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
    17521 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
    17581 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
    17641 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
    17701 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
    17761 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
    17821 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
    17881 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
    17941 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
    18001 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
    18061 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
    18121 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
    18181 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
    18241 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
    18301 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
    18361 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
    18421 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
    18481 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
    18541 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
    18601 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
    18661 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
    18721 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
    18781 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
    18841 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
    18901 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
    18961 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
    19021 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
    19081 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
    19141 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
    19201 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
    19261 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
    19321 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
    19381 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
    19441 caggcggagc ggggactggg gcaggaaacc cccgttgagt ctctgttgac gcagtaccat
    19501 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
    19561 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
    19621 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
    19681 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
    19741 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
    19801 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
    19861 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
    19921 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
    19981 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
    20041 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
    20101 tagccggtac gaatgcaatg agtaaatggc aggatcgtg
//
LOCUS       DAZPJP010000064        20128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPJP010000064 DAZPJP010000000
VERSION     DAZPJP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.064"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            201..872
                     /locus_tag="ACHU9J_002088"
     CDS             201..872
                     /locus_tag="ACHU9J_002088"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR1905622.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            882..2078
                     /locus_tag="ACHU9J_002089"
     CDS             882..2078
                     /locus_tag="ACHU9J_002089"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1905623.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2078..2638
                     /locus_tag="ACHU9J_002090"
     CDS             2078..2638
                     /locus_tag="ACHU9J_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1905624.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2640..3413
                     /locus_tag="ACHU9J_002091"
     CDS             2640..3413
                     /locus_tag="ACHU9J_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR1905625.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3601..3876
                     /gene="frmR"
                     /locus_tag="ACHU9J_002092"
     CDS             3601..3876
                     /gene="frmR"
                     /locus_tag="ACHU9J_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR1905626.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3911..5020
                     /gene="frmA"
                     /locus_tag="ACHU9J_002093"
     CDS             3911..5020
                     /gene="frmA"
                     /locus_tag="ACHU9J_002093"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR1905627.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5113..5946
                     /gene="fghA"
                     /locus_tag="ACHU9J_002094"
     CDS             5113..5946
                     /gene="fghA"
                     /locus_tag="ACHU9J_002094"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1905628.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6172..6711)
                     /locus_tag="ACHU9J_002095"
     CDS             complement(6172..6711)
                     /locus_tag="ACHU9J_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR1905629.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHU9J_002096"
     CDS             complement(6813..8024)
                     /gene="mhpT"
                     /locus_tag="ACHU9J_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR1905630.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHU9J_002097"
     CDS             complement(8311..9324)
                     /gene="mhpE"
                     /locus_tag="ACHU9J_002097"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR1905631.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHU9J_002098"
     CDS             complement(9321..10271)
                     /gene="mhpF"
                     /locus_tag="ACHU9J_002098"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR1905632.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHU9J_002099"
     CDS             complement(10268..11077)
                     /gene="mhpD"
                     /locus_tag="ACHU9J_002099"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR1905633.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHU9J_002100"
     CDS             complement(11087..11953)
                     /gene="mhpC"
                     /locus_tag="ACHU9J_002100"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1905634.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHU9J_002101"
     CDS             complement(11971..12915)
                     /gene="mhpB"
                     /locus_tag="ACHU9J_002101"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR1905635.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12917..14581)
                     /locus_tag="ACHU9J_002102"
     CDS             complement(12917..14581)
                     /locus_tag="ACHU9J_002102"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR1905636.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14623..15456
                     /locus_tag="ACHU9J_002103"
                     /pseudo
     CDS             14623..15456
                     /locus_tag="ACHU9J_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15533..16615
                     /gene="lacI"
                     /locus_tag="ACHU9J_002104"
     CDS             15533..16615
                     /gene="lacI"
                     /locus_tag="ACHU9J_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR1905637.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHU9J_002105"
     CDS             16738..19812
                     /gene="lacZ"
                     /locus_tag="ACHU9J_002105"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR1905638.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19864..>20128
                     /locus_tag="ACHU9J_002106"
     CDS             19864..>20128
                     /locus_tag="ACHU9J_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1905639.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4736 a         5519 c         5224 g         4649 t
ORIGIN      
        1 tacataccaa acatacaaac acttaacata tgaattgcat aaaaacatta cccagtcgaa
       61 cctccgggtc tgaaagccct tgcttttcag ttattagcaa catttaacat ttataaatta
      121 acatctggct gcgccagaga ttacgtctct gagtgcatcg ttgtgcttgt agtataataa
      181 ataaaatgga gtgtcattaa gtggataagg ttttagattc agccctcctt tcttcggcaa
      241 ataaaagaaa gggtatcctt gctattggcg cacatcctga cgatatagaa ttaggctgtg
      301 gcgcatcgct tgctcgtctt gcgcaaaaag gaatttatat cgcagcagtg gtgatgacta
      361 ccggcaactc tggtacagat ggaataatcg atcgccatga agaatcgcgc aacgccctaa
      421 agatattagg gtgccaccaa actattcatc ttaattttgc tgacacccgc gctcatttac
      481 agctcaatga tatgatttcc gccctggaag acatcattaa aaatcaaatt ccttctgatg
      541 ttgaaatcat gcgggtatat accatgcatg atgccgaccg ccatcaggat catctcgctg
      601 tttatcaagc ttcaatggtt gcctgccgca ctattccaca aattctcggc tacgaaaccc
      661 caagtacctg gctttcattt atgcctcagg tttttgaatc cgttaaagaa gaatatttca
      721 cagtcaaact tgctgcatta aaaaaacata aaagccagga acgacgtgat tatatgcgtc
      781 atgatcgcct gcgtgcagtt gcacaatttc gcgggcaaca ggtcaatagc gatctgggtg
      841 aaggctttgt aattcataaa atgattcttt gagcaaattg catgaaaacc tggatattta
      901 tctgtatgtc catagcaatg ttgctatggt ttttaagtac gctaagacgt aaacccagtc
      961 aaaagaaagg ctgtattgac gccattatac ctgcgtataa cgaaggcccg tgtctggcgc
     1021 agtcactgga taatctactg cgtaaccctt atttttgccg ggtaatttgc gttaacgacg
     1081 gctccacgga caataccgaa gcggtcatgg cggaagtcaa acgcaaatgg ggcgaccgct
     1141 ttgttgccgt cacgcaaaaa aataccggta aaggtggtgc gctgatgaat ggcctcaatt
     1201 atgccacctg cgaccaggtt tttttaagtg atgccgacac ctatgttccg cccgatcaag
     1261 acggaatggg ctatatgctg gcagaaatta agcgcggtgc tgatgccgta ggcggcattc
     1321 cctctactgc gttgaaaggc gcggggctgt taccgcacat ccgcgcgacc gtaaagttgc
     1381 cgatgattgt tatgaagcgc acgctacagc agctcctggg cggcgcaccg tttattatca
     1441 gcggtgcctg cggaatgttc cgtactgatg tattgcgtaa gttcggtttc tcggatcgta
     1501 ctaaagtcga agaccttgat ctcacctgga cattagtggc aaacggctac cgtattcggc
     1561 aggcgaatcg ctgcatcgta tacccacagg aatgcaacag tccgcgtgag gagtggcgtc
     1621 gctggcggcg ttggattgtg ggctacgcgg tctgtatgcg cctgcataaa aggcttttat
     1681 ttagccgctt cggtatcttc agtatatttc ctatgctgtt ggttgtgatt tatggcgttg
     1741 ggatttatct cactacctgg tttaatgaat tcatcaccac cgggccgcat ggtgtggtgt
     1801 tggcaatgtt tccgcttatc tggatcggcg tagtttgtgt tattggtgct tttagcgcct
     1861 ggtttcatcg ttgctggttg ttggtgcctt tagcgccgct ttccgttgtg tatgtattat
     1921 tagcttatgc catctggatt atctatggac ttattgcctt ttttactgga cgcgaacctc
     1981 agcgcgacaa acccacccgc tattccgcac tggtggaagc gtcaaccgct tattcccaac
     2041 cttctgtcac aggaactgaa aaactatctg aagcttaatg ttaaagagaa aaattattca
     2101 gattgcagac caggtaatta ttgatgaaac tgcaggtgaa gttgttatcg gcgcgaatac
     2161 ccgtatttgt catggtgccg ttattcaggg tccggtagtg attggcgcaa actgcctgat
     2221 aggtaattat gcgtttattc gtcctggcac aataatcagc aatggtgtaa aaattggttt
     2281 tgccaccgaa attaaaaatg cggttattga agcggaagca acgattggtc cgcaatgtta
     2341 tattgccgac tcggtagttg caaaccaggc atatttgggc gcacaagtac gtaccagtaa
     2401 tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca gagggaatta tcgctaccgg
     2461 atgcgataaa ttaggttgtt atatcgggca gcgttcacgc cttggcgtac aagttattat
     2521 tttgcctggg cgaattattt ccccgaacac gcaacttggc ccgcgcgtga ttgtagaacg
     2581 taatttacct accggaactt attcactccg acaagaactt atccgtacag gagattaaaa
     2641 tgataaaacg gacgttatta gcggcggcca tttttagcgc attgcccgct tatgccgggt
     2701 taacttccat taccgcgggc tacgatttta ccgattattc tggcgatcat ggcaaccgta
     2761 atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc aacgctgctt tttaatcttt
     2821 cccaggggcg tcgtgattat gaaactgaac atttcaatgc cactcgcggt cagggtgcgg
     2881 tctggtataa atggaataac tggctgacaa cccgaacggg tattgccttt gcggataata
     2941 cgccggtctt tgcccgccag gattttcgtc aggatattaa cctggccctg ttgccaaaaa
     3001 cgcttttcac gaccggttat cgctacacta aatattacga tgatgtcgaa gtcgatgcct
     3061 ggcaaggcgg cgtatcactc tatactggcc cggtgatcac cagctaccgc tatacccatt
     3121 atgactccag cgatgcaggt ggtagttata gcaatatgat ttccgtgcgt ctgaatgacc
     3181 cgcgcggcac tggttatacg caactatggc taagccgcgg aacaggcgct tacacctatg
     3241 actggacgcc agaaacacgc tacggcagca tgaagagcgt cagtttgcaa cgtattcaac
     3301 cgctgactga gcaacttaat ctcgggctga cggcaggtaa agtgtggtac gacaccccaa
     3361 ccgatgatta taacggtctg caacttgcag cccatctgac ctggaaattc tgattgcttc
     3421 tgccgcccgc tatccggggc ggccttccct gccgattagc cccactccct ttcctcgttg
     3481 ttttccgacc acattcaccg gataaatttt attctccagc gttatatact atagggaggt
     3541 atgcattgac atatagcata cctccctata gtatattgcg tgcagatgat gaggtgcgaa
     3601 atgcccagta ctccggaaga gaagaaaaag gtccttactc gagttcgtcg tattcggggg
     3661 cagattgatg ctctggaacg gtcgctggag ggtgatgccg aatgccgtgc catactccaa
     3721 cagatcgctg ccgttcgggg cgcggctaac gggctgatgg cagaagtgct tgaaagccat
     3781 atccgggaaa cgtttgaccg aaatgactgc tacagccgcg aagtcagcca atccgttgac
     3841 gacactattg aactggttcg agcctatctt aaatagctga atctattacc atattgagga
     3901 agagcgagag atgaaatcac gtgctgccgt tgcatttgct cccggtaaac cgctggaaat
     3961 cgttgaaatt gacgttgcac caccgaaaaa aggtgaagtg ctgattaaag tcacccatac
     4021 cggcgtttgc cataccgacg catttaccct ctccggggat gacccggaag gtgtattccc
     4081 ggtggttctc ggtcacgaag gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag
     4141 cgtcaaacct ggcgaccatg tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt
     4201 ctgtcgttct ggcaaaacta acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt
     4261 gatgccagac ggcaccaccc gtttttctta caacgggcag ccgctttatc actacatggg
     4321 gtgctctaca ttcagtgaat acaccgtggt cgcggaagtg tctctggcca aaattaatcc
     4381 agaagcaaac catgaacacg tctgcctgct gggctgtggc gtgaccaccg gtattggcgc
     4441 ggtgcacaac acagctaaag tccagccagg tgattctgtt gccgtgtttg gtcttggcgc
     4501 gattggtctg gcagtggttc agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat
     4561 cgataccaac ccgaagaaat tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa
     4621 cccgaatgac tacgacaaac cgattaaaga tgtcctgctg gatatcaaca aatggggtat
     4681 cgaccatacc tttgaatgca tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc
     4741 gcaccgcggc tggggtcagt cggtgatcat cggggtggca ggttctggtc aggaaatctc
     4801 cacccgtcca ttccagttgg tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt
     4861 gaaaggtcgt tcccagttac cgggtatggt tgaagatgcg atgaaaggtg atatcgatct
     4921 ggaaccgttt gtcacgcata ccatgagcct tgatgaaatt aatgacgcct tcgacctgat
     4981 gcatgaaggc aaatccattc gaaccgtaat tcgttactga tttcccgcag gtataccccg
     5041 tccacttcag acggggttct taatactctc cccgggcagc cgtccggggg attaaccgtg
     5101 agataatgac tgatggaact cattgaaaaa catgccagct ttggcggctg gcaaaatgtg
     5161 tatcggcatt attcccaatc actgaaatgt gaaatgaatg tcggcgtcta tctcccacca
     5221 aaagccgcga atgaaaaatt gccggtgctg tactggcttt caggcctgac ctgcaacgag
     5281 cagaatttca ttactaaatc ggggatgcag cgttacgcgg ctgagcacaa cattattgtt
     5341 gttgcgccgg acaccagtcc gcgaggcagt catgtcgcag atgccgaccg ttacgatctc
     5401 gggcaaggtg ccgggtttta cctgaacgca acgcaagcgc cgtggaatga acattacaaa
     5461 atgtatgact atatccgcaa cgagctgccg gatttagtga tgcaacattt tccggcaacg
     5521 accagaaagt ctatctctgg tcattctatg ggcgggctgg gcgcgctggt gctggcgtta
     5581 cgtaacccag atgaatatgt cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa
     5641 gtaccgtggg gacagcaagc ctttgccgca tatcttggtg aaaataaaga tgcctggttg
     5701 gattacgatc cggtgagtct tatttcacaa ggtcaacgcg ttgcggaaat catggttgat
     5761 caggggttga gtgatgattt ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc
     5821 tgccaggaga tgaatatcaa gacgttaatc cgttatcacg agggttacga ccacagctat
     5881 tattttgtct ccagttttat tggcgagcat attgcctacc acgccaataa actgaatatg
     5941 cgttgataat agtgcacgac tgccggatgc ggcgtgaacg ccttatccgg cctacacttc
     6001 gcccgtaaac cgtaggcctg ataagacgcg caaagcgtcg catcaggcat ggcgcacgac
     6061 tgccggatgc ggcgtgaacg ccttatccgg tctacacttc gcccataaac cgtaggcctg
     6121 ataagacgcg catagcgtcg catcaggcat ctgcgcacga cttatctatg tttaccacat
     6181 caaatcatca ggcactttga agtcggcata cggatcgtct tcatcctgct cttcggcact
     6241 gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat cggcaacact
     6301 ggcgggaata atggtgtatt caccttcgct attgttatcg accaacaagc gggcaatcgc
     6361 cagacgacca ttaatcaact gcgcctgtgt gagcttatcg acaaaaatct tcttaatcag
     6421 attgccgtca gtgaagttaa aaccaatatc gccattggca atggtgattc ggttcatttc
     6481 aatgagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt tttgctgttc
     6541 gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg cagcccgagc
     6601 ttcacgcgcc tgaacgcgcg attttttcgc cgttctctcg accttcgccg cttttttgct
     6661 ggtgactaat cctgctttga gcaactgctc ttgtaaggta agttttgcca tttttgtttc
     6721 caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtgggtg tattgggggc
     6781 aagtcccttt cgcccctgct taataaggcg cgtcaggcat cggcacacgg ctgcattctt
     6841 gatctccgac tcatcaaaat aaacaccgcc aacccagcaa caagaatacc cggtgcagaa
     6901 gcggccatta cgccgaccgt gccagtgcct aatgccagca ttttcccggc cagtaacgga
     6961 ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt tgcgcggatc
     7021 tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc tgtcgcaaac
     7081 aaccccgcga caaatcccgc cagcaacata ccgttaaatg acgacaccgt tccaagcgcc
     7141 agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg acgcagctta
     7201 tccatcaatg cgcccaacat taacgtcccg cttgccgccc ccatttgcag ggcaaacatc
     7261 acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagtagcgg tagccagttg
     7321 atcaacatat aaaccaccag cagagtgaag aaataacaca accacagcag cagcgtcgcg
     7381 gttgccgttt ctggcgcaaa taaggcacgc agtggtggcg cagcctgttt ttcgccagcg
     7441 aaaaccgctg actccggcag ccagcgcatt aatagcggca ccagaatcaa cggcaccaca
     7501 ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc cagtgtcgcc
     7561 gccagcgccg cgccaatggg aacaccgcaa tacatcaggc tcactgccgt cccacgaaaa
     7621 cgtggacccg cggcttcaga tgtcagggcg ataagattcg gcaacgccgc ccccagcccg
     7681 acaccggtca tcagccgcgc aaagaccagt gaggggaaat cccaggcaat cgccgttgcc
     7741 agtgagaaca aaccaaacag cgcaactgag ccaatcaaaa tacgcttacg accataacgg
     7801 tctgccagca tcccgccaac caacgcgccg ggtagcaaac cgagtattcc ggcgctaaat
     7861 atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc cgccgcaatg
     7921 ccagccgcct gaagatccag cccttccatc agagcgacca aaaaacaaag cccgatggtc
     7981 agcatcaggc gggatgaaga tgatgaaggg gtacgagtcg acataagatg aacctcattg
     8041 cgagtgcagg tgaatcgcga ctgatacatt gccagccgcg taatcagaac tatttaatga
     8101 ctgcctacgg gcgaaatgta gcgaaatgta ggccggataa ggcgttcacg ccgcatccgg
     8161 cagtcgtgcg cagatgcctg atgcgacgct aacgcgtctt atcagaccta caaattctca
     8221 cgccatccgt aggccggaca aggcgttcac gccgcatccg gctgtcgtgc tgggatgggc
     8281 atacaaaatg cacgttatcg gcagtcatca ttatttgttg ttgcgcagat ccagcgccac
     8341 gtcgacgatc atatcctcct ggccgccaac catccggcgt ttgcccagct caacgagaat
     8401 atccaccgca cttaagccat aacgcgccgc cgccgtttca cagtgacgca ggaagctcga
     8461 gtaaacacca gcgtatccca gcgccagcgt ttcgcgatcg actcgtaccg gacgatcctg
     8521 caacggacgc accaggtcgt cggcggcatc cattaacgca tagagatcgg tcccatgctg
     8581 ccagcccagt ttatccgcgg cggcaataaa cacttccagc ggtgcgttac ctgcgcccgc
     8641 gcccattccc gcgaggctgg cgtcgattcg gtcgcagccc tcttccaccg ccgcgataga
     8701 gttcgccacg ccaagactca ggttatggtg agcgtgcatg ccggtttgcg tttctggttt
     8761 caacactgct ttcagggcgc ggaaacggtc acggatatcg ctcatgttca tcgcaccgcc
     8821 agaatccacc acataaatac aggtcgcacc gtagccttcc atcagctttg cctgcttggc
     8881 gagattctcc ggcgtggtca tatggctcat catcagaaaa ccaacggtgt ccattccgag
     8941 ctcgcgggca tactgaatat gctgggcgga aacatcagct tcggtacagt gcgttgccac
     9001 acgaaccacc cgcgcgccag cctgccaggc atttttcaga tcgtgaatag tgccgattcc
     9061 tggcagcaac aacgtcgcga ttttggcgtg cctcaccaca tccgccgccg cttcaatcca
     9121 ttcaaggtcg ctatgtgcgc cgaaaccata gttaaagctg gaaccttgca aaccgtcgcc
     9181 gtgggccact tcaatcgaat ccacgcgggc atcgtccagt gctttggcaa tctggcgaac
     9241 gttttccagc gaatactgat gacgaatggc gtgcataccg tcacgcaatg tgacgtccga
     9301 gatataaagt tttttaccgt tcatgccgct tctcctgcct tgcgcgccag tgactgggcc
     9361 attttttccg ctgtcgccag cgcactggaa gtcataatat cgaggttgcc cgcataggca
     9421 ggcagataat gcgctgcgcc ttcgacttcc agccagaccg ctgttttcag tccggagaat
     9481 tgccccacgc ccggtaaatt gaccggttta tcctgcggga taacttcaaa ctgcacgcgc
     9541 tgtttcaggc gataacccgg tacgtaagcc tgcaccgcct cagccatttc attgattgag
     9601 gcttcgatat catcttgtga agcttcgtcg ctcaatacat acaccgtgtc acgcatcatc
     9661 aacggtggct ctgctgggtt aagcacaata atcgccttcc cttttgccgc gccgcccacc
     9721 acttcaatgg ctcgggaagt ggtttccgta aattcatcga tattggcacg cgtgccaggt
     9781 ccggcagatt tactggcgat agaagcgata atttcggcgt aatgaacacg cgccacgcgt
     9841 gaaactgccg ccaccattgg aatggtggcc tggccgccgc aggtgaccat gttgacgttc
     9901 agttgatcga cgttcgcctc gaggttaacc accggcacgc agtaagggcc gatggcagca
     9961 ggcgtcaggt caattaagcg aatatccggt ttcgcttcgc gtaaagcggc atcgtttttc
    10021 acatgcgcgc ccgcgctggt cgcatcaaat acaatgtcga tatcagcaaa ttcaggcatg
    10081 ttcatcagcc cgatcacccc ttcatgggtg gtggcgacgc ccatacgtct ggcacgcgcc
    10141 agaccgtcgg actgaggatc aatgccgacc atcaccgcca tctccagatg ctgaccgtga
    10201 cgcaaaattt taatcatcag atcggtgcca atgttgccag aaccgataat ggcgacttta
    10261 cgcttactca tgacagactt ccttttgggg ctgcgcttga aaatgtcgca gcaactgaac
    10321 ctatgccttc aatatgggct tcaaaacgat cgcccgcatt caccgccacc atcgggccta
    10381 atgccccggt aagaatgata tctccggcgc gcagcggttc acccagactg gccattttgc
    10441 gtgccagcca gacggcggca ttaagcggat gtcccaggca ttcgctgccg cgcccgctgg
    10501 aaacctcttc gttattacgc gtcatcttca tggcgcagtt tttcaggtct aaccccgccg
    10561 gacgttgcgc cggaccgccg atgacataca ccccgcagga agcgttatct gccacggtat
    10621 cgacaaactg aatcgaccag tcgcgaatgc ggctccccac cacttccagc gccggaagta
    10681 cccattcaat ggcgttatac agttcgtcga aggtgatatc agttgcgggt aaatcgcggt
    10741 tcaacaccag tgcgatctcc gcttcaatgc ggggttgcag aacacgggaa aaaggaatga
    10801 tttcgttatc gccataacac atgtcggcaa ataacgtccc aaaatccggt tgatcaacgc
    10861 ccagttgttg ttgcactttc ggatgcgtca ggcccacttt acgccctacc acgcgacgcc
    10921 cctgcacaac gtcatattgc acatttatgt gctgaatggc gtaggccgct tcagcgtgat
    10981 cgataccaat cagatcgcgc agcggcgcaa tcgcttcgcc ctgctctgcg gcgcggcgta
    11041 aatccgccgc cagttgctca agagtatgct tcgtcatgac cattccttaa gggcgtgcga
    11101 ggaaattcag caccaattga ttgaaagcgt cggcatgttc ccactgcgcc cagtgaccac
    11161 agtcgcggaa gatatgcagt tcagaaccgg caatgccgga cagcagacgc agacccgcat
    11221 ccatcggcac aaagcggtcg ttgcgccccc agacaatcag ggtttgcgct ttgatttccg
    11281 ccagacgtgg gccaaaatcc gggaactgtt tcggattagc ttccaggctc ttaacgaagt
    11341 tttccaggtg atcgcggcgc gacagcatat tattcaggcg cgcttcaaac agggcgtcgg
    11401 tcaaatcgct ggtatcgaaa acgaagatat ccatcatcag cttcaggttt tcgatagtcg
    11461 gctgacgata aagctgattc agtcgcttaa taccttcggt tggcatcggc gtaaacaaac
    11521 tcatgccgcc cgtaccgccg cccatcagca ccagtttgcc gacgcgctcc ggccagttaa
    11581 gggtgaacgc cacagaacta tggccgccca tcgagttgcc cagcaggtgg attttggcga
    11641 tatccagttg atccaccacg cttttcagga ttcgtgcatt aagatccgat cgtgaaccac
    11701 tattaacgat cgaatcgctt ttgccccaac ccggacaatc cagcaggatc acccgatagc
    11761 ccgcctctac cagcggatcg atattgcggc tgaagttcgc ccagccagta gcacccgggc
    11821 cggaaccatg cagcaggaca acggtttcgt cgccttgtcc gcagtcatta aaatggatgc
    11881 gcagcgtttt acccgcttct tctacattca gaaaacggct ggtggcggct tcggtttgtg
    11941 gctgataact catcatcttc tcctgcatat tcagttctct gttctggcgc ttaacgagcc
    12001 aaatccggca atccactccg ggattgggcg gtaataacgc ccttcgctac gccagttgcc
    12061 aaatgcagaa atagcggcaa aagcggcgac ccaggttttg atttcatgtg tcgactttcc
    12121 ggcaatggcg gaaagctctt cgttactgac ggcatccagt tcctgtatgc gtccctgctc
    12181 cagcaaagtc atgaactggt tatcccaaat cgggttgagc ggatgcagag ttctctgatc
    12241 ctcaacaaac ttctcagcgg cgctaatcac cctttgctga cgcaattcgc gctcactggc
    12301 gggtaaatct ttcccgctcc ccaacagacg gtcgcgcata tgggcatcgg ctttcgccag
    12361 ttcgggcacc ggcggctgat gggaaagccc accggaaccc aggaacagca cgcgtttatt
    12421 gagagtgctg gtgaaacgtc caatggcttc acccaacatg cgggtacgct ggaaaccggg
    12481 cagcggcgtg gcgacaccgt tgatgaacac aggcagaact ggcaccttat ccagcccacc
    12541 gagcaggaac tccagcggct gggcgaaccc gtggtccacc tgcatacagt aagaaacggc
    12601 aagatcgatc ccgctcttca tgacagcatg cgcacaggcc tccgccagct ccacaggcac
    12661 gggcagctct cctgccgcac tgccgaaatc accaattgcc gtcgctccaa cgcctaaaca
    12721 gaacggtggc atcacgtcat agaaaaagcc gttgtagtga tctggcgcaa acagcaccac
    12781 cagttcaggg gagaaagccg caatacgctc gcgggcgctg gcaatcacgc cattgacctc
    12841 atcgagtacc tcttgcgccg ggtcgacata ccccaccaac ggcgagtggg aaagacagtg
    12901 aagataagcg tgcatatcag gctacctttt cgacagaaac gtcggcatca gggcgggtca
    12961 gcgtcatcac cgacgccagt ttattcagcg tattgcccag ggtttgcgga atggcggtgg
    13021 cggcaacaaa gcgatccggg cgcatcacca ccagcgaagc attgtgttgc gcgaaccagc
    13081 tacgcagacg accttgcgta tcgcccacgt gtagcacgcc gtcgtggtta tcctgtgcgg
    13141 tatgaatttg cacttccggc accacctgaa tgaagcgtgt gcccaacgcg cgccactgct
    13201 ggatttgctc gtcgctcatc ccccacagtg gattgcatcc ccagccaatt accgcgaagt
    13261 tcgcgccgat cgcgttatcg agcagcgtca cgtcgccgtt ttccagcgtg actttcggct
    13321 gaataaacat cttgccgacc ggagagtgct tcgcttcgcc ctcacgcacc agcgcaccgc
    13381 cgtaatattg cggcatcggc ttgaagcgca tttcgaggaa gtagcgtttt actggcggca
    13441 gataattcaa cagccaggaa acgccgtcac gtaacgtacc ctgccagcgt ttcggcggag
    13501 ccagcacgtt gcccgccgtc acggacaggt caatcatcgc tttggcgtga tcgcgacgtt
    13561 cttgttgata ggtatcgagc agcgcatcgc gggctttccc ctggataacc aacgccagtt
    13621 tccaggcgag gttaaaggcg tcgcgcatac cactgttata gccctgcccc tgccacaccg
    13681 gcatgatgtg cgcggcatcg cccgccagca gtacgcgatc aatacggaaa cgttgcgcca
    13741 gtcgcgcgtt atgggtgtag acacgctggc gaatcaattc aacattgtcc ggattaggca
    13801 gcactttgct taacagcttg cgcatatttt gcggctcacg cagctgctct tcggtttctc
    13861 ccggcatcac cataaattca aagcgacgta ccgcatgagg cagcgcggca gaaacatacg
    13921 ggcgcaccgg atcgcaacac aaatagatat gcggcgtact taacggatcg ttggcgatat
    13981 ctaccacaat ccactgattt ggcgcagttt taccttcaaa cggcacattc agggtgcgac
    14041 gaacaaaact tgccccacca tcacaggcca ccagccactg ggctttgatc gtttcccgct
    14101 gcccttctgc cgttttcagg tgcaaggtca cttcgtcatc ttgctggctg aaggcctcca
    14161 gctcgcggga aaacaagcag cgcacattcg gaaaacgcga caacccttcc agcatcaccg
    14221 catcgacctg tggctgaata aaggcgttac ggcgcggcca gccaaattca tcggtcattg
    14281 gctgaatatc agcaaaacag cggccttttg gggtgagaaa acgcatcgcg tgccacggtg
    14341 tagtgtgcgg cagaacattc tcgaccaggc cgaccgactg catggtgcgc agcgcctcgt
    14401 catcaatacc aatcgcacgc gggtagtcga tcaacttatc gagtttctcc accaccagca
    14461 cgtcaatgcc catctgaccg agatagttcg ccatcatcag cccaaccgga ccggcaccag
    14521 cgatcgccac ctgaacgcta tggttaacag caggctggat gtcagggtgt tgtattgcca
    14581 tttctgcaca acgttgtttt aactatagaa atgtcaatta atatgcagaa caatgagcag
    14641 acggaataca aaaccgtgcg cggcttaacc cgcggtctaa tgttattaaa tatgttaaat
    14701 aaacttgatg gcggtgccag cgtcgggctg ctggcggaac tcagcggcct gcatcgcacc
    14761 actgtgcggc gactgctgga gacgctgcag gaagagggat atgtccgccg tagcccctcc
    14821 gatgacagtt ttcgtctgac catcaaagtg cggcaattaa gcgaaggatt tcgtgacgaa
    14881 cagtggattt ctgcactggc ggccccgctg ctgggcgatc tgttgcgcga agtggtatga
    14941 ccaacagatg tgtccaccct ggatgttgat gcaatggtgg tacgcgaaac cacccaccgt
    15001 ttcagccgct tatcctttca ccgggcaatg gtcgggcgac gtttaccgct cctgaaaacc
    15061 gcctcgggcc tgacctggct ggccttttgc ccggaacaag aacgcaagga attaatcgaa
    15121 atgttagccg cccgccccgg tgatgactat caactggcac gagaaccgtt aaagctgcaa
    15181 gccattctgg cgcgcgcgcg caaagagggt tacggacaga actaccgcgg ctgggatcag
    15241 gaggagaaga tcgcctctat cgccgtaccg ctgcgcagtg aacaacgggt gattggctgt
    15301 ctgaatctgg tgtatatggc gagcgcaatg accattgaac aggcagcgga aaagcatctt
    15361 ccggcgctac aacgggtagc aaaacagatc gaagaagggg ttgaatcgca ggctattctg
    15421 gtggccggac ggcgaagcgg cgtgcattta cgttgacacc atcgaatggc gcaaaacctt
    15481 tcgtggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
    15541 agtaacgcta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttctcgcgt
    15601 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
    15661 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
    15721 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
    15781 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
    15841 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
    15901 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
    15961 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
    16021 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
    16081 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
    16141 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
    16201 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
    16261 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
    16321 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
    16381 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    16441 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    16501 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
    16561 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    16621 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    16681 cggctcgtat gttgtgtgaa attgtgagcg gataacaatt tcacacagga aacagctatg
    16741 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16801 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    16861 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
    16921 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
    16981 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
    17041 accaacgtga cctatcccat tacggtcaat ccgccttttg ttcccgcgga gaatccgacg
    17101 ggttgttact cgctcacatt taatattgat gaaagctggc tacaggaagg ccagacgcga
    17161 attatttttg atggtgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
    17221 tacggccagg acagccgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
    17281 aaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gtagttatct ggaagatcag
    17341 gatatgtggc gaatgagcgg cattttccgt gacgtctcgt tgctgcacaa accgagcaca
    17401 caaatcagcg atttccatgt tgccactcac tttaatgatg atttcagccg cgcggtactg
    17461 gaggcagaag ttcagatgta cggcgagctg cgcgatgagc tgcgggtgac ggtttctttg
    17521 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
    17581 gagcgtggtg gttatgccga tcgcgtcacg ctacgtctga acgtcgaaaa cccggcgctg
    17641 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgacgacggc
    17701 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    17761 ctgctgctgc tgaacggcaa gccgttgctg attcgcggcg ttaaccgtca cgagcatcat
    17821 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    17881 cagaacaact ttaacgccgt gcgttgttcg cattatccga accatccgct gtggtacacg
    17941 ctgtgcgacc gcttcggctt gtacgtggtg gatgaagcca atattgaaac ccacggcatg
    18001 gtgccaatga atcgtctgac cgatgatccg cgctggttac ccgcgatgag cgaacgcgta
    18061 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    18121 gagtcaggcc acggcgctaa tcacgacgca ctctatcgct ggatcaaatc tgtcgatcca
    18181 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    18241 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    18301 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    18361 gcgatgggta acagtcttgg cggcttcgct aaatactggc aggcgtttcg tcagtacccc
    18421 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    18481 aatggcaatc cgtggtcggc ttacggcggt gattttggcg atacaccgaa cgatcgccag
    18541 ttctgcatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacagaagca
    18601 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    18661 gaatacctgt tccgtcatag cgataacgag cttctgcact ggtcggtggc gttggatggc
    18721 aagccgctgg caagcggtga aatgcctctg gatgttgctc cacaagataa acagttgatt
    18781 gaactgcctg aactaccgca gccggagagc gccggacaac tctggcttac ggtacacgta
    18841 gtgcaaccga acgcgaccgc atggtcagaa gccggacaca tcagcgcctg gcagcagtgg
    18901 cgtctggcgg aaaacctcag cgtgacactc cccgccgcgc cccacgccat cccgcaactg
    18961 accaccagcg aaacggattt ttgcatcgag ctgggtaata aacgttggca atttaaccgc
    19021 cagtcaggct ttctttcaca gatgtggatt ggcgctgaaa aacaactgct gacgccgctg
    19081 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    19141 attgacccga acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    19201 gcgttgttgc agtgcacggc agatacactt gccgacgcgg tgctgattac gaccgctcac
    19261 gcgtggcagc atcagggaaa aaccttattt atcagccgga aaacctaccg gattgatggt
    19321 agtggtcaaa tggcgattac cgttgatgtt gaagtagcga gcgatacacc gcatccggct
    19381 cggattggcc tgacctgcca gctggcgcag gtagcagaac gggtaaactg gctcggatta
    19441 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    19501 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    19561 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caatatcagt
    19621 cgctacagcc aacaacaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    19681 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    19741 ccgtcagttt cagcggaatt ccagcttagc gccggtagct accattacca gttggtctgg
    19801 tgtcaaaaat aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc
    19861 attatgtact atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac
    19921 ttttttatca tgggggccta cttcccgttt ttcccgattt ggctacatga catcaaccat
    19981 atcagcaaaa gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc
    20041 caaccgctgt ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt
    20101 attaccggca tgttagtgat gtttgcgc
//
LOCUS       DAZPJP010000065        19908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPJP010000065 DAZPJP010000000
VERSION     DAZPJP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.065"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            112..171
                     /gene="ibsE"
                     /locus_tag="ACHU9J_002107"
     CDS             112..171
                     /gene="ibsE"
                     /locus_tag="ACHU9J_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1905640.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(291..1724)
                     /gene="hldE"
                     /locus_tag="ACHU9J_002108"
     CDS             complement(291..1724)
                     /gene="hldE"
                     /locus_tag="ACHU9J_002108"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1905641.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1772..4612)
                     /gene="glnE"
                     /locus_tag="ACHU9J_002109"
     CDS             complement(1772..4612)
                     /gene="glnE"
                     /locus_tag="ACHU9J_002109"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1905642.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(4635..5936)
                     /gene="ygiF"
                     /locus_tag="ACHU9J_002110"
     CDS             complement(4635..5936)
                     /gene="ygiF"
                     /locus_tag="ACHU9J_002110"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1905643.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6178..6798
                     /locus_tag="ACHU9J_002111"
     CDS             6178..6798
                     /locus_tag="ACHU9J_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1905644.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6862..8100
                     /gene="cca"
                     /locus_tag="ACHU9J_002112"
     CDS             6862..8100
                     /gene="cca"
                     /locus_tag="ACHU9J_002112"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1905645.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8263..9084)
                     /gene="bacA"
                     /locus_tag="ACHU9J_002113"
     CDS             complement(8263..9084)
                     /gene="bacA"
                     /locus_tag="ACHU9J_002113"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1905646.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9175..9543)
                     /gene="folB"
                     /locus_tag="ACHU9J_002114"
     CDS             complement(9175..9543)
                     /gene="folB"
                     /locus_tag="ACHU9J_002114"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1905647.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            9648..10265
                     /gene="plsY"
                     /locus_tag="ACHU9J_002115"
     CDS             9648..10265
                     /gene="plsY"
                     /locus_tag="ACHU9J_002115"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1905648.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10278..11210)
                     /gene="ttdR"
                     /locus_tag="ACHU9J_002116"
                     /gene_synonym="dan"
     CDS             complement(10278..11210)
                     /gene="ttdR"
                     /locus_tag="ACHU9J_002116"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR1905649.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            11417..12328
                     /gene="ttdA"
                     /locus_tag="ACHU9J_002117"
     CDS             11417..12328
                     /gene="ttdA"
                     /locus_tag="ACHU9J_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1905650.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            12325..12930
                     /gene="ttdB"
                     /locus_tag="ACHU9J_002118"
     CDS             12325..12930
                     /gene="ttdB"
                     /locus_tag="ACHU9J_002118"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1905651.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12979..14442
                     /gene="ttdT"
                     /locus_tag="ACHU9J_002119"
     CDS             12979..14442
                     /gene="ttdT"
                     /locus_tag="ACHU9J_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1905652.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14485..15498)
                     /gene="tsaD"
                     /locus_tag="ACHU9J_002120"
     CDS             complement(14485..15498)
                     /gene="tsaD"
                     /locus_tag="ACHU9J_002120"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1905653.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15735..15950
                     /gene="rpsU"
                     /locus_tag="ACHU9J_002121"
     CDS             15735..15950
                     /gene="rpsU"
                     /locus_tag="ACHU9J_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1905654.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16061..17806
                     /gene="dnaG"
                     /locus_tag="ACHU9J_002122"
     CDS             16061..17806
                     /gene="dnaG"
                     /locus_tag="ACHU9J_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1905655.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18001..19842
                     /gene="rpoD"
                     /locus_tag="ACHU9J_002123"
     CDS             18001..19842
                     /gene="rpoD"
                     /locus_tag="ACHU9J_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1905656.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4711 a         5560 c         5130 g         4507 t
ORIGIN      
        1 tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa tcggggagtc agcaaggggc
       61 tgaaacggga aagcccctcc cgaggaaggg gccttataga aggaaagggt tatgatgaag
      121 ttcgtcatca tactggttgt gttgttactg ttaagtttcc cgacttacta acaactgatc
      181 agagggggaa gaaatcctcc cttacccttg ttcctttact ttaggttgaa aaaacaacag
      241 cgtcaatagg cctgcctgct acaaagcgag atctgtgaac cgctttccgg ttagcctttt
      301 ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc
      361 accagcactt cgccgccgtt ggcccagact tctttactcc cggcgatctc ttctggttta
      421 tagtcgccgc ccttcaccag taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg
      481 tcttcttcaa acgacaccac ccagtcgacc gcttccagtg cgcccagcac aatcatacgc
      541 tgttcgagcg ggttaacggg gcgggaatcc cctttcagcc gtttggtgga ggcatcgctg
      601 ttcacagcga caatcagacg gtcacccagt ttgcgagcat ttgccagata ggagacgtgc
      661 ccggcgtgca ggatgtcaaa gacaccgttg gtcattacca ccttttcgcc gcgtttacgc
      721 gctgccgcta cggccagctt cagttcctct tcggtcatca cgccaaagcc ggtatctgcg
      781 cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg
      841 accaccacgc cagccgccgc attggcaaag aagcaggctt cttccagcga attacccgct
      901 gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg caccggtaac gtcatacact
      961 tcctgcgcct gggttggcat atgcagcggc gctttacccg gttgcagcag cgacataccc
     1021 tgttcggaac gggtcactaa cagagccgag agttcgtaat cggcaatcag tttcatgccg
     1081 cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga
     1141 ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat cggtaccttt tggatcaatc
     1201 agcaccggaa cgcccgcttt acgcgccagt tggatcatct gctgtacgct tgccagcgca
     1261 cctttggcgt agtcagaaag caccagcgcg ccaatcgaac tcagcgcctg attaatccgc
     1321 tcgtgcagcg gctgcggatc aacgccttcg aaaccttctt cgaaatccag acggatcagc
     1381 tgttggttgc gggaaagtac ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg
     1441 aagtcgcatt tgacgttgac gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca
     1501 atgcccgtca acccgaccag gcgtgcatta gcaccgagag aagcgatatt catcgccacg
     1561 ttagccgcgc cgcccggacg ttcttcgatg gtattcactt taaccacggg caccggcgct
     1621 tccggcgaga tacggctgga gggaccgtac cagtaacgat ccagcattac atcaccaacc
     1681 accatcactc ctgcacgttc aaactctggc agcgttactt tcattcctgt ctcctgagag
     1741 attcaaaatt tgcgcgcgat aataccatac ttcattcttc caccagccac ttctgccagc
     1801 ttgcccgcac cagatcacgc tctgcggtga agcaatcccc cgacacatgg cccggcaatt
     1861 cctgtaatgc cagatgatga agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca
     1921 tcgcttcctg ctcttccata atgtcgtttt gcgccagcag ctccagaata cgcacgttgt
     1981 ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg caacaccaga tattgggtaa
     2041 taaattcgat atcggtaatt ccgccttcat cagctttgat atcaaagcga tcgcgatgtt
     2101 tattgccgag atgagcgcgc attttctcgc gcatttcccg cacttccgtt tgcagagttc
     2161 taccttcacg cggcagcgtc ataatctcgc gacgcactgc gtcaaagtgc gcggtgagct
     2221 gcggatcgcc gtacactacc cgcgcacgca ccagcgcctg atgttcccac gtccaggcct
     2281 cgtttttctg ataatcggca aatgcttctg tggaggtaac cagcattccc gccgccccgg
     2341 acgggcgcag ccgcgcatcc acttcgtaca aaatgccgga agaggttcgc gtgctgaaca
     2401 ggtgcataat gcgttgcgcc agacgaagat aaaactgtcg tccgtcgatt tcccgctcgc
     2461 cgtcagtcat cgcatccatt gggcaatcat ggaggaagat caggtcaaga tcggaactgt
     2521 agcctaactc ccagccgccc agcttgccgt agccgaccac tgcaaaacca cgcccttcgc
     2581 gatcgttgag gtgattcggc ttaccgtagc gggcaaccat ttgaacccac gcctgctgaa
     2641 cgacggcatc tatcatggct tccgccagcc aggttaagtg atcgctcact ttcatcaccg
     2701 gtagcgtacc agcgatatcc gctgcggcga tgcgtaacag ctgcgcctgt ttgaactgac
     2761 gcagcgcctc aagctgttgc tcttcgtcat cttccggcac gcgcagcaaa tactggcgca
     2821 actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag agtgtttgga tcgagcaatt
     2881 catccagcaa taatggataa cgcgccagct ggctggcaat catcggcgat gcggcacaca
     2941 gagaaatcaa atggttaagc gccgcaggga attcactgag caattctaaa taggtggtgc
     3001 gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac
     3061 gcgcacagac atcacttagc agatgcggca tcagatggtc gagcacctga cgtcctcgcg
     3121 gcccgatggt gcgcttatcc agctctttgc ggaaatcgga aatcagcgtt agcacctgtt
     3181 tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat
     3241 cctgccacag ctcacgccac tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt
     3301 cgccaatcaa ttcattaaac acccggcgca cattgttcat atgtgcggtc agcgccccgg
     3361 tcagttgcgg ccagtcagca aagtccatcg cccacgccag ccgcgcacgg ttaagctcat
     3421 cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg cagcaggttt tccagacgcc
     3481 gcaggaacag atacgccact cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg
     3541 cagcaatggc gctgagcgtt ggcagtaaag agcacgattg cagcgacggt tcgcgcccgc
     3601 cgcgaatgag ctggaatacc tggacgataa attcaatttc gcgaatacca cccgcgccaa
     3661 gtttgatgtt gtcggtcaaa ccgcgccgac gcacttcacg ggcaatcatt cctttcatgt
     3721 tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg gaaaacaaac gggcgtagca
     3781 tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc cataatccgc gctttgacca
     3841 tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata atcctccaac gcggcaaagc
     3901 tcagcaccag cgggccactt tcgccaaacg gacgcagtcg catatccacg cgatagacga
     3961 agccatcctg cgttggttga tccagcactt taatcagccg ctgccccatg cgggtaaaaa
     4021 actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa
     4081 aaatcagatc gatatcagag gagaaattca gttccccgcc gcccagctta cccatgccta
     4141 agatcagcag cggttgcgct tcgccctgcg cattgcacgg cgttccccac tcgcggcagc
     4201 aggcgtcata cagccagtca cgcgccgcaa caatcagcgt ctccgccaga taactgagct
     4261 gctgcaatat gctctcttca gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca
     4321 taatgcgccg ccggaatagc cgcagctcgc gcattaaccc ggcttcgtca ctcacattac
     4381 tgagcgcctc ctgcaaccat gcagcgtaat gctgccattc atcggcctgc ggcggttggc
     4441 tttccagttc cgtcaaccac tctcgatgcg caattatgct gtcctgcaca aaatcactga
     4501 aagtaagtac tgacttcgcc tgtgcactta gtggttcctc ggctaaaggc tctggcagcc
     4561 gctcaacaac ggtctgccag tactgctgta acggtgaaga gagcggcttc ataagcgatt
     4621 ttatccttgc ctgattaacg ttttccgctg tgcaaccaga acggttcctg attgtttgcc
     4681 tcattacgga aatgctcaat ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc
     4741 tgccagttct ccagccaggc atgcgcgacg acaggatcat agtaacccgc cagcaacagt
     4801 attgagtcaa tgtcacgcgt caggcgtggc aactggtcac ggtagagatc gcctaacggc
     4861 tggcagaaaa cgcttttcag ttcagcggca tggtgggaaa gatggatatc ggcaaagcgt
     4921 ttgaaggagt cgctgatttt gccctgcgct ttggcatcta aaaatggctg ccatgctttg
     4981 cttaccaacc attcggtcaa cgccagcttc gccattgccg tttcggtaga gtagaccgcc
     5041 gtcacggcag aaaccgcaga agcaatggtc gcttcgcatt gggtcagcaa atcacgtaag
     5101 tgagtgctcg ctttacgcgg cacaatacca ccgaacagca tcagcgtatg acggaccagg
     5161 ctaatggctg ccagcacctg ttctttcgcc gcatcgttac cgcgtaccca cagttcttca
     5221 tgatactgcc attgcgctaa cgccagctcg agcgccgctt ccagcccctg ttctacatcg
     5281 gcttttgccg caacatgcaa aatggtggtc ggtttgattt cacgcgccgg attgccctgc
     5341 gccagatgat agccacgcgc cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat
     5401 accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc cgctaagcag ttccagttcc
     5461 agctcacaga taggttcagc aaattccccc gctttcacct ccccctgatc gagagcgatt
     5521 tcaattcggc tgccatcgac cgccaccagc catttttcgc gataaaaatc ggtgctgaac
     5581 agcggttgta cgcgggaagc gagatcggca ggcaattcac cgttcggcca gacttccgag
     5641 ggaaactgcg ccaggtcgag cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg
     5701 tgtaagccgc ctgtcactct tcctgcaact ttcatggtca tctcatagcg accgttttcg
     5761 ccacgaatac gtaagcccat atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa
     5821 atattcagca actgcacggg gtcatggtgc tcgccgccca gcgtattgag atggtcacgc
     5881 aacgcctcaa cggcactgtg attaacaata aactttaatt cgatttcctg agccatggcc
     5941 tttacttatc ggttatgtca cataagtgac gatgaacggc gaacttaatg cgatcttttt
     6001 tgtcagtaga tagtattttg cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa
     6061 agtttgaagc agtggcaaga cctgacacag aggattccga ttgatgtcga atgctttgcg
     6121 tagcgacact gatgccacta ctatcgttcc actttccatg acaataacga cagcctgatg
     6181 ccaaaattac gcctgatcgg attaacttta ctcgcactta gcgcgactgc cgtctcacac
     6241 gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct gggtccgtag cggtccggga
     6301 gatcattatc gcctcgtggg cacggttaac gccggcgagg aagtgacctt attacaaact
     6361 gacgccaaca ccaattatgc ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg
     6421 ttgaaacaac ttagcactga gccaagcctg cgctcccgtg tgccagatct ggaaaatcag
     6481 gtcaaaaccc tgaccgataa actcaccaat atcgataaca cctggaatca gcgcaccgca
     6541 gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca acgggttaaa agaagaaaat
     6601 cagaaactga aaaacgagct gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag
     6661 ctggatgaca aacagcgcac catcatcatg caatggttta tgtatggcgg tggcgtgctg
     6721 gggcttggct tgctgctcgg tctggtactg ccgcacctga tcccaagccg caaacgcaaa
     6781 gatcgctgga tgaactaaat cgccttcttc gttgcactgt cttattatta ggaattgtgt
     6841 atttgacgaa gggatgatgg cgtgaagatt tatctggtcg gtggtgctat tcgggatgca
     6901 ttgttagggc taccggtcaa agacagagat tgggtggtag tcggcagtac gccacaggag
     6961 atgctcgacg cgggctacca gcaggtaggc cgcgattttc ctgtgtttct acatccgcaa
     7021 acgcatgaag agtatgcgct ggcacgtacc gaacggaaat ccggttccgg ttacaccgat
     7081 tttacttgct atgccgcacc ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg
     7141 accattaatg cgctggccca ggacgataac ggcgagatta tcgacccgta caacggtctg
     7201 ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg cttttggcga agatccgtta
     7261 cgcgtactgc gcgtggcgcg ttttgctgcg cgttatgccc acctcggttt tcgtattgcc
     7321 gatgaaactc tgacgctgat gcgcgagatg acccatgcgg gtgaactgga acacctgacg
     7381 cctgaacggg tatggaaaga gacggaaagc gccctcacca cccgcaatcc acaggtgttc
     7441 ttccaggtac tgcgcgattg cggcgcactg cgcgttttat tcccggaaat tgacgcactg
     7501 tttggcgttc cggcccctgc caggtggcat ccggaaatcg acacgggtat tcatacctta
     7561 atgacgctct caatggcggc gatgctgagt ccgcaggtcg atgtccgttt cgcaacttta
     7621 tgccacgatc tcggtaaagg actgacgccg ccagaacttt ggccgcgtca tcatggtcac
     7681 ggtccggcgg gtgtgaaatt agtggaacaa ctctgccagc gtctgcgcgt gccaaatgaa
     7741 attcgcgatt tagccagact ggtggctgag tttcacgatc tcatccacac cttcccaatg
     7801 ctgaacccaa aaactatcgt caaattgttt gattccatcg acgcctggcg taaaccgcag
     7861 cgtgtcgagc aactggcgct gaccagcgag gctgacgtac gcggcagaac cggttttgaa
     7921 tcagcggact acccgcaagg ccgttggttg cgcgaagcct gggaagtggc gcagtcagtg
     7981 ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg agattcgcga ggagctgacc
     8041 cgacggcgga ttgcggcggt agccagttgg aaggaacaac gttgcccaaa gcctgactga
     8101 gtataaaact aaacgttaac acgaaccatc ccctcgcccc ttcggggaga gggttagggt
     8161 gaggggaaca gaccagcacc ggtgcgaaca atcaccccca ccccggccct ctccctacag
     8221 agcgagggag aaaaccaggc ctgctcccga accttttctc aatcaaaaga acacgacgta
     8281 caccgcagcc gccacaataa agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat
     8341 caattgcagg aaggttttaa tcgctatcag cgccaccaca aaagcggtga taaacccaac
     8401 ggcaaacatc gagatatcgc cggttgtcag gaagccccag cttttgtaga gatcgagcgc
     8461 cgttgcgccc atcatcatcg gtaccgccag caggaacgag aactcggaag cagcgtaacg
     8521 gctcacgccc atcagcatcc cacctgaaat ggtggccccg gaacgggaga aacccggcca
     8581 cagcgccaga cactggaaac agccaatcat aaacgcctga cgataggtca tatcatcaag
     8641 acccggcgca cgcggctctt tcggcttcag gcattcggcg gcaatcagca acaaaccgcc
     8701 aacgaccagt gcatacatca catttatcgg gttaaacaat gacttaatcg tgtcgtggaa
     8761 caacagcccc agtaccaccg ccggaatcat ccccagcaaa atgtggatca gcgttaaacg
     8821 acctttgctt tctccttcgt gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa
     8881 cagacgccgc cagaacatca ctactaccgc cagaattgat cctaactgga tcacaacttc
     8941 aaaggttttc gccgtgtcgc cctcgaaccc caacaagtga ccgacaataa tcatatggcc
     9001 cgtgctggat accggcagaa attctgtcaa tccttcgacc acacccaata ttgccgctat
     9061 cagcagcgag tgcatatcgc tcatcaataa acccctaaat tattaaaatg taccgcttgt
     9121 ccggactact gcagtaagac caggttataa ccgtttggtt taacagctgt aaaattaatt
     9181 attttctttc agattattgc cacgctcaat gattacacca acattcgccg cccgcgccat
     9241 tgcgcctggc ttgctgagtt tgatgcgcac ccacggcgag ttgaagcgtg ctaacagcag
     9301 ctccgccacc tcttcagcca cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct
     9361 gaccaccgtt tctgcaatgt cagcgtaact gaggcaatcc gccacatcat cacttttcgc
     9421 cgctttacgg ttatcccatg ccatttcgat atcgaacact aacttctgtt cgatggtctg
     9481 ttcccagtcg taaacaccaa tagtggtgat taccgaaagt tgctctataa atacaatatc
     9541 catcacgtcc tgcctgcttt ttggctaacc ggataccact tccggcgaaa tgtgcgtatt
     9601 atccacagat tcatcgttga acacgaattt tcaaaacgga acagcttatg agtgcaatcg
     9661 cgcctggaat gatcctcatc gcgtacctct gcggctccat ttccagtgcc attctggttt
     9721 gccgcttgtg tgggctgccc gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca
     9781 atgtgttacg tatcggtggc aagggagcag ccgtagcagt actgattttc gacgttctga
     9841 aaggaatgtt gcccgtctgg ggcgcgtatg aattaggtgt cagccccttc tggttaggct
     9901 taattgccat cgccgcctgt cttggacaca tctggcccgt tttcttcgga tttaaaggag
     9961 gaaaaggcgt tgctaccgct tttggtgcca tcgcacccat tggctgggat ctcaccggag
    10021 taatggcggg aacctggtta ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga
    10081 ttgtcagtgc actgattgct ccgttttatg tctggtggtt taagccacaa ttcaccttcc
    10141 cggtttcgat gctctcttgc ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt
    10201 ggcgtcgtca ggagacaaaa atctggacga aattcaaaag aaagcgcgaa aaggatcccg
    10261 agtgatttct ggtggatcta catgacctga tagccttcat cgggcttgcc cagccgttgc
    10321 tggcaccatg ccgccagaaa ttccacgcag acacgtaatt tcatgctgcg atagagcggc
    10381 tcccggtaaa cagcccagat attggcgctt tgtgcatact ccggcaatac ttgcaccagt
    10441 ttgccactct ccagaaacgg caacacatcc cactcggaac gcagcataat ccctttgccc
    10501 tccagcgccc attgcaacac tatctcgccg ctattggagg aaagatgccc gctaaccttc
    10561 accgattttt tctcctgacc gttccccaac tcccatattc cgtgggtcat atcgcgctct
    10621 ttggtcacca gacagtcatg acgacttaat tcctgtaatg attgtggctg cggatatttt
    10681 tgcagatact caggtgctgc gcataatatt cttttatttt tcgttaacag atgcgcaata
    10741 taataatcag gaatttcgtc attaatacga atatccagat caatattatc ctgcactaaa
    10801 tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact caggataatt gcgcataagt
    10861 tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc
    10921 atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga ccagtcgctg atattgggtg
    10981 aggatttcca gcgcatgttc gtaacaacgt tggccacttt cagtcagcgc cacgccgcga
    11041 gcggagcggt tcagcagcgt tgttgccagc gtattttcaa ggatttggat ccgcttggtg
    11101 acaaatgcag gcgtctgccc aagcgtcgcc gctgcggcgc taaaactccc gctgtggacg
    11161 atttctacca gtacctgcaa gtctttggct aaaggccagc tattcagcat cgttaattat
    11221 ccgcagttgt gataagcgca gtgtatttcg caaaacattg ccattccctg ctctggcgca
    11281 atattcattc ttattttcgc gttgattcac gaattgttaa ttacatattc acggtggcaa
    11341 aaaatataaa accacatttt tagtggtagt ttgtggcggt gaatttttcc agacaaatac
    11401 aaaaactgga gttgccatga tgagcgaaag taataagcaa caggcagtga ataagttgac
    11461 ggagattgtc gctaacttta ccgccatgat ttctacccga atgcctgatg acgtggtgga
    11521 taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg gggaaaatta tctaccatac
    11581 gatgttcgac aacatgcaaa aagcgattga cctgaatcgt cctgcctgtc aggacaccgg
    11641 ggagattatg ttcttcgtta aagtcggttc ccgcttccca ctgcttggcg agctgcaaag
    11701 catactcaaa caagccgtgg aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt
    11761 agaaattttt gacgaagtaa acaccggcaa aaataccggt agcggcgtac cgtgggtcac
    11821 ctgggacatc atccccgaca atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg
    11881 ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt
    11941 gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac gcctgtccac cggtactggt
    12001 gggcgtgggc atcgccacct cggtggaaac cgccgccgta ctctcgcgta aagccatttt
    12061 gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca gaactggagc tacgcctgga
    12121 agaaggactc aaccgtctgg ggattggtcc acaagggctg actggcaaca gttcagtgat
    12181 gggcgtacat atcgaatctg ccgcccgcca tccgtcaacc atcggcgttg ctgtctctac
    12241 cggctgctgg gcgcatcgtc gcggcacact gctggttcat gccgatctca cctttgaaaa
    12301 tctgtctcac acccggagcg cgttatgaaa aagatcctga caaccccgat caaagctgaa
    12361 gatctgcaag atattcgcgt cgacgatgtg atctacctga ccggtacgct ggtgacctgc
    12421 cgtgacgttt gtcaccgccg cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac
    12481 ggcaaagcga ttttccacgc tggccccatc gtgcgcaaaa acggcgataa atgggagatg
    12541 gtttccgttg gcccgacaac cagtatgcgt atggaaagtt ttgaacgtga atttatcgag
    12601 cagaccggcg tgaaactggt ggttggcaaa ggcggaatgg ggccgctgac cgaagaaggc
    12661 tgccagaaat tcaaggcgct acatgtgatt ttcccggcag gctgcgcggt gctggcggca
    12721 acccaggtgg aagagattga agaagtgcac tggacagagc tcggaatgcc ggagtcgctg
    12781 tgggtctgtc gggtcaaaga gttcggcccg ctgattgtct ctattgatac ccacggcaac
    12841 aacctgatag ccgaaaacaa aaagctgttc gccgaacgcc gcgatcccat cgtggaagag
    12901 atctgcgagc acgtccatta catcaaataa ccctcccgga gaggctcgcc ccctctcctt
    12961 tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatacct tgcgccgctg
    13021 gcggtcatcg ccattattgc tctaattccg gttcccgcag ggctggagag tcatacctgg
    13081 ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc cgtgccgggt
    13141 gccgtggtgg cgatggtggg tatatccatc atcgccatac tctctccctg gctgctgttc
    13201 agcccggagc agctcgctca gccaggcttt aaattcactg caaaatccct ctcgtgggcc
    13261 gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat gtttggcaca
    13321 ggctatgaaa aaaccgggct tggacgccgc attgcgctga ttctggtgaa aaagatggga
    13381 catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct cgcacctgta
    13441 acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg taacctgcca
    13501 ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc gtacatcatg
    13561 tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc gatggcacca
    13621 aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag ttggggcgac
    13681 tggttcctcg ggatgttgcc gctcagtatt ttgctggtgc tgctggttcc ctggctggct
    13741 tatgtgctgt acccgccggt actgaagtct ggcgatcagg tgccgcgctg ggcagagacg
    13801 gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg gctgatagta
    13861 ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat ggtcggttac
    13921 agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat tgtcagtaat
    13981 aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc taccggactc
    14041 aacaacaccg gttttattag ctggtttggc aaactgttag caggcagctt aagcggttat
    14101 tcgccaacga tggtgatggt ggcgttgatt gtggtgtttt atctgctgcg ctactttttc
    14161 gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgattgccgc tgcgctggcg
    14221 atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat tggtctgggg
    14281 agcattctta caccatacgc caccggaccc agtccgattt actacggtag tggttatctg
    14341 ccaacggcgg attactggcg actgggggcg atttttgggc tgatattcct cgtgttgctg
    14401 gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt aagtgaggac ggtgccggaa
    14461 attgctctcc ggcacccaat agttttacgc agccggtaac tccgccagcg gccagcgcgg
    14521 acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca ttccggcata
    14581 ggcgatcatc gcgccgttat cagtacaaaa ctccggacgc gcgtagaaca cttcgccgcg
    14641 gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg cggttagcac tcacgccgcc
    14701 cgccatgacc agtcgcttaa agcccgtctg atccaacgct cgcttacact taatcatcaa
    14761 cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct ggtcgtcggt
    14821 gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc tgaaatccag
    14881 ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac cctgagccgc
    14941 cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg cggttttatc
    15001 aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact gaccaatgcc
    15061 agtcacgctg attaactgcg tatgaccgcc ggacaccagc agcgcgacaa acggaaattc
    15121 cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt gtaccgggat
    15181 tgccggaacg ttccaggcaa acgccagaga acgccccacg gttgcgccaa ccagcagtgc
    15241 gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg ccgttaagcc
    15301 agactccttt agcgccgcct ggatcaacgg tacggttttg cgcacgtgat cgcgggaggc
    15361 cagttcaggc acgacgccgc catagtcagc gtgcaatttc acctgactat acaattggtt
    15421 ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc aggaagtttc
    15481 aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag tgtaggacca
    15541 atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat actcctcacc
    15601 cttataaaag tccctttcaa aaaggccgcg gtgctttaca aagcagcagc aattgcagta
    15661 aaattccgca ccattttgaa ataagctggc gttgatgcca gcggcaaacc gaattaatca
    15721 aaggtgagag gcacatgccg gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc
    15781 tgcgtcgctt caagcgttcc tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg
    15841 agttctatga aaaaccgact accgaacgta agcgcgctaa agcttctgca gtgaaacgtc
    15901 acgcgaagaa actggctcgc gaaaacgcac gccgcactcg tctgtactaa ttccccgaga
    15961 gcgttgctct ccgatcagac cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg
    16021 cggcttattt tcgtttatga attgctaaaa atcggggcct atggctggac gaatcccacg
    16081 cgtattcatt aatgatctgc tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt
    16141 gaagctgaaa aagcagggca agaatttcca cgcgtgttgt ccattccaca acgagaaaac
    16201 cccgtcattc accgttaacg gtgagaaaca gttttaccac tgctttggat gtggcgcgca
    16261 cggcaacgcg atcgacttcc tgatgaacta cgacaagctc gagttcgttg aaacggtcga
    16321 agagctggca gcaatgcaca atcttgaagt gccatttgaa gcaggcagcg gccccagcca
    16381 gatcgagcgc catcagaggc aaacgcttta tcagttgatg gacggtctga atacgtttta
    16441 ccaacaatct ttacaacaac ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg
    16501 attaagccac gaggttatcg ctcgctttgc gattggtttt gcgccccccg gctgggacaa
    16561 cgtcctgaag cggtttggcg gcaatccaga aaatcgccag tcattgattg atgcggggat
    16621 gttggtcact aacgatcagg gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc
    16681 cattcgcgat aaacgcggtc gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac
    16741 ccccaaatac ctgaactcgc cggaaacaga cattttccat aaaggccgcc agctttacgg
    16801 tctttatgaa gcgcagcagg ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta
    16861 tatggacgtg gtggcgctgg cgcaatacgg cattaattac gccgttgcgt cgttaggtac
    16921 gtcaaccacc gccgatcaca tacaactgtt gttccgcgcg accaacaatg tcatttgctg
    16981 ttatgacggc gaccgtgcag gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc
    17041 ttacatgaca gacggccgtc agctacgctt tatgtttttg ccggatggcg aagaccctga
    17101 cacgctggta cgaaaagaag gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc
    17161 gctttccgca tttctgttta acagcctgat gccgcaagtt gatctgagta cccctgacgg
    17221 gcgcgcacgt ttgagtacgc tggcactgcc attgatatcg caagtgccgg gcgaaacgct
    17281 gcgaatatat cttcgtcagg aattaggcaa caaattaggc atacttgatg acagccagct
    17341 tgaacgatta atgccaaaag cggcagagag cggcgtttct cgccctgttc cgcagctaaa
    17401 acgcacgacc atgcgtatac ttatagggtt gctggtgcaa aatccagaat tagcgacgtt
    17461 ggtcccgccg cttgagaatc tggatgaaaa taagctccct ggacttggct tattcagaga
    17521 actggtcaac acttgtctct cccagccagg tctgaccacc gggcaacttt tagagcacta
    17581 tcgtggtaca aataatgctg ccacccttga aaaactgtcg atgtgggacg atatagcaga
    17641 taagaatatt gctgagcaaa ccttcaccga ctcactcaac catatgtttg attcgctgct
    17701 tgaactgcgc caggaagagt taatcgctcg tgagcgcacg catggtttaa gcaacgaaga
    17761 acgcctggag ctctggacat taaaccagga gctggcgaaa aagtgattta acggcttaag
    17821 tgccgaagag cgatcgggaa gcccccgaca gccgcactga gaggcagcgg caaatatata
    17881 agtacgccct cgtaattatc gttggcggta aacaaccgtt ggatttcagc gttaacggct
    17941 gaaggacatc gggtcaatcg cccaacacca acctcatgaa ataagtgtgg ataccgtctt
    18001 atggagcaaa acccgcagtc acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc
    18061 tatctgacct atgccgaggt caatgaccat ctgccggaag atatcgtcga ttcagatcag
    18121 atcgaagaca tcatccaaat gatcaacgac atgggcattc aggtgatgga agaagcaccg
    18181 gatgccgatg atctgatgct ggctgaaaac accgcggacg aagatgctgc cgaagccgcc
    18241 gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg
    18301 tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct
    18361 aagcgtattg aagacgggat caaccaggtt caatgctccg ttgctgaata tccggaagcg
    18421 atcacctatc tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat
    18481 ctgatcaccg gctttgttga cccgaacgca gaagaagatc tggcacctac cgccactcac
    18541 gtcggttctg agctttccca ggaagatctg gacgatgacg aagatgaaga cgaagaagat
    18601 ggcgatgacg acagcgccga tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa
    18661 tttgcggaac tacgcgctca gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc
    18721 agtcacgctg ccgctcagga agagatcctg aaactgtctg aagtattcaa acagttccgc
    18781 ctggtgccga agcagtttga ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt
    18841 cgtacgcaag aacgtctgat catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa
    18901 aacttcatta ccctgtttac cggcaacgaa accagcgata cctggttcaa cgcggcaatt
    18961 gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc
    19021 ctgcagaaac tgcagcagat tgaagaagaa accggcctga ccatcgagca ggttaaagat
    19081 atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt
    19141 gaagcgaact tacgtctggt tatttctatc gccaagaaat acaccaaccg tggcttgcag
    19201 ttccttgacc tgattcagga aggcaacatc ggtctgatga aagcggttga taaattcgaa
    19261 taccgccgtg gttacaagtt ctccacctac gcaacctggt ggatccgtca ggcgatcacc
    19321 cgttctatcg cggatcaggc gcgcaccatc cgtattccgg tgcatatgat tgagactatc
    19381 aacaaactca accgtatttc tcgccagatg ctgcaagaga tgggccgtga accgacgccg
    19441 gaagaactgg ctgaacgtat gctgatgccg gaagacaaga tccgcaaagt gctgaagatc
    19501 gccaaagagc caatctccat ggaaacgccg atcggtgatg atgaagattc gcatctgggg
    19561 gatttcatcg aggataccac ccttgagctg ccgctggatt ctgcgaccac cgaaagcctg
    19621 cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt
    19681 atgcgtttcg gtatcgatat gaacaccgac cacacgctgg aagaagtggg taaacagttc
    19741 gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac
    19801 ccgagccgtt ctgaagtgtt gcgtagcttc ctggacgatt aatcggtagg ccggatcagg
    19861 cgttacgccg cacccggcac tagtccctct gcacaaacgc cacctttt
//
LOCUS       DAZPJP010000066        19132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPJP010000066 DAZPJP010000000
VERSION     DAZPJP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.066"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(225..1874)
                     /gene="pgi"
                     /locus_tag="ACHU9J_002124"
     CDS             complement(225..1874)
                     /gene="pgi"
                     /locus_tag="ACHU9J_002124"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1905657.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2111..2307
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2399..3748
                     /gene="lysC"
                     /locus_tag="ACHU9J_002125"
     CDS             2399..3748
                     /gene="lysC"
                     /locus_tag="ACHU9J_002125"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1905658.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            4000..4272
                     /locus_tag="ACHU9J_002126"
     CDS             4000..4272
                     /locus_tag="ACHU9J_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1905659.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4405..5277)
                     /gene="rluF"
                     /locus_tag="ACHU9J_002127"
     CDS             complement(4405..5277)
                     /gene="rluF"
                     /locus_tag="ACHU9J_002127"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1905660.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDDERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5625..6462
                     /locus_tag="ACHU9J_002128"
                     /pseudo
     CDS             5625..6462
                     /locus_tag="ACHU9J_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6532..7338
                     /locus_tag="ACHU9J_002129"
     CDS             6532..7338
                     /locus_tag="ACHU9J_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1905661.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7348..7755
                     /locus_tag="ACHU9J_002130"
     CDS             7348..7755
                     /locus_tag="ACHU9J_002130"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1905662.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7755..8249
                     /locus_tag="ACHU9J_002131"
                     /pseudo
     CDS             7755..8249
                     /locus_tag="ACHU9J_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8315..9112
                     /locus_tag="ACHU9J_002132"
     CDS             8315..9112
                     /locus_tag="ACHU9J_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1905663.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9123..9947
                     /locus_tag="ACHU9J_002133"
     CDS             9123..9947
                     /locus_tag="ACHU9J_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1905664.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9999..11227
                     /gene="sorE"
                     /locus_tag="ACHU9J_002134"
                     /pseudo
     CDS             9999..11227
                     /gene="sorE"
                     /locus_tag="ACHU9J_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11610..12299
                     /gene="pepE"
                     /locus_tag="ACHU9J_002135"
     CDS             11610..12299
                     /gene="pepE"
                     /locus_tag="ACHU9J_002135"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1905665.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12390..14021)
                     /locus_tag="ACHU9J_002136"
     CDS             complement(12390..14021)
                     /locus_tag="ACHU9J_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1905666.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14241..17924)
                     /gene="metH"
                     /locus_tag="ACHU9J_002137"
     CDS             complement(14241..17924)
                     /gene="metH"
                     /locus_tag="ACHU9J_002137"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1905667.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18124..18948
                     /gene="iclR"
                     /locus_tag="ACHU9J_002138"
     CDS             18124..18948
                     /gene="iclR"
                     /locus_tag="ACHU9J_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1905668.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4509 a         5004 c         5011 g         4608 t
ORIGIN      
        1 taaaaatcta ttttaggatt aacctcacgg tatgatttcc gttaaatcac agacaagggg
       61 atttatctga taaaacaatg ccgacttatt gtcggcattg tttttaaggt tatttaactc
      121 ctcatactat gcattaatgt cgatattggc atcaggctcc ggttgccgga tgcggcgtaa
      181 acgccttatc cggcctacat atcggcatca ggcacgcatg atgattaacc gcgccacgct
      241 ttatagcggt taatcagacc attggtcgag ctatcgtggc tgctgatttc tttgtcatct
      301 ttcagctctg gcagaatacg gttcgccagt tgtttaccca actccacgcc ccactggtcg
      361 aaggtaaaga tgttcaggat cacgccctga gtaaagattt tgtgctcata cagcgcaatc
      421 aacgcaccca ggctgaacgg agtgatttcg cgcagcagga tggagttggt cgggcggtta
      481 ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg ccggatcttt accctgatcg
      541 cgatattcct gctcaaccac ttcgcgggat ttaccaaacg ccagcgcttc ggtctgggcg
      601 aagaagttag acagcagttt ctggtggtga tcagagagcg ggttatgggt gatagccggg
      661 gcgatgaaat cgcacggtac cattttggtt ccctggtgga tcagctggta gaacgcgtgc
      721 tgaccgttag tgcctggttc accccagata atcgggccag tctggtaatc cacaacgtta
      781 ccgttacggt caacatactt accgttggac tccatattgc cctgctggaa gtacgccgcg
      841 aaacggtgca tatactggtc atacggcaga atcgcttcag tttccgcacc aaagaaattg
      901 ttgtaccaga tgccaatcag cgccagcagg acaggcaggt ttttctcggc aggcgtggtg
      961 gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt caacgaagtt atcaaagccg
     1021 atggagagaa caatcgacag gccaatcgct gaccacaaag agtaacggcc gccgacccag
     1081 tcccagaact cgaacatgtt ggcagtatca ataccaaact cgccaacggc tttggcattg
     1141 gtggaaagcg ccgcaaagtg ttttgcaacg tgtttttcat cacctgccgc tttcaggaac
     1201 cagtcacgcg cgctatgggc gttggtcata gtttcctgag tggtgaaggt tttagatgct
     1261 accaggaaca gcgtggtttc cgggtttact tttttcagca cttcagcgat gtgagtccca
     1321 tcgacgttag aaacaaagtg catgttcagg tgatttttgt acggacgcag tgcttcggtc
     1381 accatgtacg ggccgaggtc agaaccaccg atcccgatgt tcactacgtc agtgattgct
     1441 ttgccggtat aacccttcca ctcaccggag ataatcgctt ccgagaaggt tttcatcttc
     1501 tccagcaccg cgttgacttc cggcattacg tctttgccat caaccagaat cggggtattg
     1561 ctacggttac gcagcgctac gtgcagcacg gcgcggtttt cagtgcggtt gatcttctcg
     1621 ccagagaaca tcgacttaat cgcgcccgcc agatcgcact ctttcgccag atcctgtaat
     1681 ttcgccagcg tctcttcagt gatgcggttt ttggagtaat ccaccagcat ctgatcgtcg
     1741 aatgttgcgg agaacttaga aaaacgatcg ccgtctttag caaaaagatc ggcgatcgta
     1801 acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc aggcagcggt ctgcgttgga
     1861 ttgatgtttt tcattagcaa tactcttctg attttgagaa ttgtgacttt ggaagattgt
     1921 agcgccagtc acagaaaaat gtgatggttt tagtgccgtt agcgtaatgt tgagtgtaaa
     1981 cccttagcgc agtgaagcat ttattagctg aactactgac cgccaggagt ggatgaaaaa
     2041 tccgcatgac cccgtcgttg acaaccgccc cgctcaccct ttatttataa atgtactacc
     2101 tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa cggtgttgga ggagccagtc
     2161 ctgtgataac acctgagggg gtgcatcgcc gaggtgattg aacggctggc cacgttcatc
     2221 atcggctaca ggggctgaat cccctgggtt gtcaccagaa gcgttcgcag tcgggcgttt
     2281 cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa gtatcgctct gcgcccaccc
     2341 gtcttccgct cttcccttgt gccaaggctg aaaatggatc ccctgacacg aggtagttat
     2401 gtctgaaatt gttgtctcca aatttggcgg taccagcgta gctgattttg acgccatgaa
     2461 ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt ttagtagtcc tctcggcttc
     2521 tgctggtatc actaatctgc tggtcgcttt agcggaaggg ctggaacctg gcgagcgatt
     2581 cgaaaaactc gacgctattc gcaacatcca gtttgccatt ctggaacgtc tgcgttaccc
     2641 gaacgttatc cgtgaagaga ttgaacgtct gctggagaac attactgttc tggcagaagc
     2701 ggcggcgctg gcaacgtctc cggcgctgac agatgagctg gtcagccacg gcgagctgat
     2761 gtcgaccctg ctgtttgtcg aaatcctgcg cgaacgcgat gttcaggcac agtggtttga
     2821 tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt gcagagccag atgtagccgc
     2881 gctggcggaa ctggccgcgc tgcagctgct cccacgcctc aatgaaggct tagtgatcac
     2941 ccagggattt atcggtagcg aaaataaagg tcgtacaacg acgcttggcc gtggaggcag
     3001 cgattatacg gcagccttgc tggcggaggc tttacacgca tctcgtgttg atatctggac
     3061 cgacgtcccg ggcatctaca ccaccgatcc acgcgtagtt tccgcagcaa aacgcattga
     3121 tgaaatcgcg tttgccgaag cggcagagat ggcaactttt ggtgcaaaag tactgcatcc
     3181 ggcaacgttg ctacccgcag tacgcagcga tatcccggtc tttgtcggct ccagcaaaga
     3241 cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa aatccgccgc tgttccgcgc
     3301 gctggcgctt cgtcgcaatc agactctgct cactttgcac agcctgaata tgctgcattc
     3361 tcgcggtttc ctcgcggaag ttttcggcat cctcgcgcgg cataatattt cggtagactt
     3421 aatcaccacg tcagaagtga gcgtggcatt aacccttgat accaccggtt caacctccac
     3481 tggcgatacg ttgctgacgc aatctctgct gatggagctt tccgcactgt gccgggtgga
     3541 agtagaagaa ggtctggcgc tggtcgcgtt gattggcaat gacctgtcaa aagcctgcgg
     3601 cgttggcaaa gaggtattcg gcgtactgga accgttcaac attcgcatga tttgttacgg
     3661 cgcatccagc cataacctgt gcttcctggt gcccggcgaa gatgccgagc aggtggtgca
     3721 aaaactgcat agtaatttgt ttgagtaaat actgtatggc ctggaagcta tatttcgggc
     3781 cgtattgatt ttcttgtcac tatgcttatc aataaacgag cctgtactct gttaaccaac
     3841 gtctttatcg agaataattg cctttaattt ttttatctgc atctctaatt aattatcgaa
     3901 agagataaat agttaagaga aggcaaaatg aatattatca gttctgctcg caaacgaatt
     3961 cccgtacact gaactctctg gctcactgag ggcaccatca tggcactccc ccgtattacc
     4021 caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa cactgctgga tcgggcgcgg
     4081 attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca gcattaagaa agagtacatc
     4141 gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaag cccgccaatt gaagaaaaag
     4201 caggcttata aaccggatcc tgaagcgtca tttagctggt cagcaaatac gtcgacgcgc
     4261 ggcaggcgct aatgaacaat caacgcctga gattttcact cgtcagtgtg aacacaattt
     4321 tcccctcacc ctaacccttt tcccgaaggg gcgaggggac tgtccgagca cttttgggcc
     4381 gttttcatca gtctgaggcg ttaatcagcg ccccttcttt ttacgccccg gcgaggtaaa
     4441 acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca tcttaacgac
     4501 tggacgtttg atgcccgctg ttttcggttt ggccttcgct ttgggcttcg cctcggaaga
     4561 ggaattttca atgagcttaa agagatcgat aagctcgtcg tcggttaaat cgcgccattc
     4621 tcccagcgga atgccgctta agctgacgtt cataatgcgc gtacgttcca gctttttcac
     4681 ttcatagccg aaatgctcgc acatgcgccg gatctgacgg ttcaacccct gcaccagggt
     4741 aatgcggaag acaaacggtg cttctttttt gactttgcac tttttcgtca ccgtcccgag
     4801 gatcggcact cccgccccca taccacgaat aaattcatcg gtaatcggtt tatcgaccgt
     4861 caccagatac tctttctcat gatcgttacc cgcacggagg atcttattca ccagatcgcc
     4921 gtgattggtg aggaaaatca gcccctggga gtctttatcc aggcggccaa tcgggaacac
     4981 gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgtcatctt cggtggtgct
     5041 gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggctt cccgaggctc
     5101 aatcaactga ccgtttactt tcacaatgtc gccgggtttc acctgatcac caatggtggc
     5161 tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt cgcggcgtga
     5221 gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg gcagcataaa
     5281 ttctcctgta aaacgcggaa tataccgtac ctcttagcgg ggaaaaacag ggttatgcgg
     5341 caacgtcgaa attaaatgtg atctgccacg ccttttatca caaaagtgtc tccggaagta
     5401 cgatgcaaat ttgcctggca aaataaggat aatcaacata ttacgaatcg ctgcacacct
     5461 gtgctgatga ctactacgca gagaaacaaa tgtgcaagag aaagcgctat ttctccccat
     5521 gcaaagagtg aaaaatcgct ttgaaaggcc ctcataatgg ggttatatca gatcaagcgg
     5581 tgagaaagcg tatttgcaca aatgtgcaga accaggaggc ggcaatggaa aacagtgacg
     5641 atatccgttt gattgtgaag attgcccaac tctattacga acaggatatg acgcaggcgc
     5701 aaatcgcgcg cgaactgggt atttaccgca ccaccatcag ccgcttgctt aaacgaggcc
     5761 gcgatcaggg aattgtcacc atcgccatca actatgacta caacgaaaat ctctggctgg
     5821 agcagcaact gaagcaaaag tttggcttga aagacgttgt ggtggtgtcg ggaaatgatg
     5881 aggatgaaga gactcaactg gcgatgatgg ggttacacgg cgcgcaactg ctggatcgct
     5941 tgctggagcc tggcgatatt gtcggttttt cctagggtcg cgcggtgagc gcactggttg
     6001 aaaacttgcc gcaagcgggg caatcgcggc agttaatctg cgtgccaatt attggcggac
     6061 cgtccggtaa actcgaaagc cgctatcacg taaacacatt aacctacagc gtggcagcga
     6121 agctgaaagg ggaatcgcat ctcgcggatt ttccggctct gctggataac cccttaattc
     6181 gtaatgggat catgcagtct cagcacttta aaaccatctc tgcctactgg gataatctgg
     6241 atgtcgccct ggtgggaatt ggctcaccgg ccattcgcga cggcgctaac tggcatgcgt
     6301 tttatggtgg tgaagagagt gacgacctga aagcaggcgc ggtattccat tggcattgcc
     6361 atgagtgaag aaaaatacag cggaattgtt ggtgcactgc gtggaaaata tattaattgt
     6421 ctggtaacga atagcagcac agctgaacta ttactgaaat aaaaatcttc ccgatgttta
     6481 ccgtaataaa aattacggcg ggtccctatt atctaaaatc aggagtctgt tatgcaaacg
     6541 tggttaaatt tgcaggataa aatcattatc gtcaccggcg gcgcatccgg tattggtctg
     6601 gcgattgtag aggaattatt agcacaaggc gcaaacgtac agatggtaga tattcacggt
     6661 ggcgatggtc aatatgaagt tcataaaggc tatcagtttt ggccgaccga tatttccagc
     6721 gccaaagagg taaatcatac ggtagcggaa attatccagc gttttggtcg catcgacggt
     6781 ctggtcaata acgccggggt caatttcccg cgtctgctgg tcgatgagaa agcgcctgcc
     6841 gggcagtatg aactcaacga agctgcattc gaaaaaatgg tcaatatcaa ccaaaaaggc
     6901 gtttttctga tgtcgcaggc ggtggcgcgc cagatggtca aacaacatga tggcgtgatt
     6961 gtgaatgttt cctcagaaag tggactggaa ggctcagaag gccaaagctg ttacgccgct
     7021 accaaagccg cgctcaatag cttcacgcgc tcctggtcga aagagctggg taaacacggt
     7081 atccgtgtgg tcggtatcgc gccggggatt ctggaaaaaa ccgggctacg cacgccggaa
     7141 tatgaagagg cgctggcgtg gacgcgcaat atcaccgtcg agcagctgcg tgaaggctat
     7201 accaaaaacg ccattcctat tgggcgcgcc ggaagattag cagaagtcgc tgattttgtt
     7261 tgttatctgc tgtctgaacg cgccagctac atcaccggag taaccactaa cattgcgggc
     7321 ggcaaaacgc gcggataagg aggctttatg gtcaacgcca ttttttgtgc ccacggcaaa
     7381 ttagcttgcg ccatgctgga atcggttcag atggtgtacg gcgacgccaa tgtcgaggcg
     7441 gttgaatttg taccaggcga gaacgccgga gacatcgtag caaagctgga aaagttagta
     7501 agcattcaca atcaagatga gtggctgatt gccgtggatt tacagtgcgg cagcccgtgg
     7561 aatgcggcag cgatgcttgc gatgcgcaat cctcgtctgc gggtaatcag cggcctttcc
     7621 ctgccgctgg cgctcgaact ggttgataac caggacagca tgaatgttga tgaactgtgt
     7681 gaacacctga cgcaaatcgc aaagcaaacc tgcgttgtct ggaaacaact ggcaacggca
     7741 gaggaagatt tctgatgaac attacgctcg cccgcattga tgaccgtctg atccacggcc
     7801 aggtcactac cgtctggtcg aaagtggcta acgcccagcg cattattatc tgcaatgacg
     7861 aagtttataa cgatgaagtg cgccggacat tattgcgtca ggctgcgcca ccgggaatga
     7921 aggttaacgt ggtcaatatt gaaaaagccg tcgcggttta tcacaacccg caatattagg
     7981 acgaaaccgt tttttatctt ttcactcgcc cacaggatgc cttagcgatg gttcgccagg
     8041 gggtaaaaat aggcaccctg aatattggcg gcatggcctg gcggcccggt aaaaaacagt
     8101 taaccaaagc ggtttcatta gatgatgatg atattaacgc atttcatgag ttaaataatc
     8161 ttggcgtcat aatggattta cgcgtcgtag cgtcagatcc ctcaatcaat attatcgaca
     8221 aaataaacga acagttaatc gcaaattaaa taatagcgcc tgtgtacata tgctttaaca
     8281 ggcagggatg acgacactta aaaggtgaca aatcatggaa ataagtaccc tacaaattat
     8341 tgctatattt cttttttcct gtattgccgg aatgggcagc gtgctggatg aatttcagac
     8401 tcatcgcccg ttaattgcct gtacggtgat tggtttaatt ctcggtgatt taaaaaccgg
     8461 aataatactc ggtggtacgc tggaattgat agctctcggc tggatgaacg tcggcgcggc
     8521 gcaatctccg gattctgcac tcgccagcat aatctccgcc attctggtta tcgttggtca
     8581 gcaaagcatc gccaccggaa tcgccatcgc gttgcctgtg gctgcggcag gccaagtgct
     8641 gacggtgttt gcccgtacca tcaccgtagt gttccagcac gcggcggata aagcagcgga
     8701 agaggcgcgg tttcgcactc tcgatattct gcatgtctcc gcgcttggcg tgcaggcgct
     8761 gcgcgttgct attccggcac tgattgtctc actgttcgtc agcgccgata tggtcagcaa
     8821 tatgttaaac gccattcccg aatttgtgac ccgtggactg caaattgctg gcggttttat
     8881 cgtggtggtc ggttacgcca tggtgctacg tatgatgggc gtgaaatatt tgatgccttt
     8941 ctttttcctc ggtttcctcg caggtggcta cctcgatctc agcctgctgg cgttcggtgg
     9001 cgtcggcgtg atcatggccc tggtctacat ccagctaaat ccacagtggc gtaaagctga
     9061 acctcaactc caggccaccg cctccaccgc ccttgaccaa cttgacgatt aacggagcca
     9121 tcatggaaca gagaaaaatt acacgcagcg atctggtgag catgtttctg cgctccaacc
     9181 tgcaacaggc gtcctttaac tttgaacgta ttcacggact gggcttttgc tacgacatga
     9241 tccccgccat caagcgactt tacccattaa aagaggatca ggttgcggcg ctcaggcgac
     9301 acctggtgtt cttcaatacc acgccagccg tatgtggccc ggtcatcggc gtcactgccg
     9361 ccatggaaga ggcgcgggcc aacggcgcgg aaattgatga cggtaccatt aacggcatca
     9421 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     9481 gcccgattac cgctgcgctc ggcgcatctc tggcactttc tggcaacatc ctcggcccgc
     9541 tgctgttttt ctttattttc aatgcggtgc gtctggcgat gaagtggtat ggcctacagc
     9601 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cgggaatgtg ctgcaaaaac
     9661 tcaccgaagg cgcgtcgatt ctcggactgt ttgtgatggg cgtgctggtc accaaatgga
     9721 cgtcaatcaa cgtaccgttg gtggtttcac aaacgcatgc cgccgatggc tccaccgtca
     9781 tcatgaccgt gcagaacatt ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     9841 cgctactgat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     9901 tgtttggctt agggattatc ggcaatgcgc tgggcttcct gtcctgattc ttcgccccgg
     9961 cacgactgcc ggggccatcg ctcaacatga ggtggtttat gaaaacaaca gctctgcgtc
    10021 tttatggtaa acgtgattta cgcctggaaa cctttgacct tcctgaaatg caggaggatg
    10081 aaatcctcgc gacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggcaacct
    10141 gggtgaaaac cataaaaaag tacccgacga tgtggcgacc aacccaatca tcatcggcca
    10201 cgagttttgc ggcgatattc tggccgtggg taaaaagtgg cagcacaaat tccagccggg
    10261 tcagcgttat gtgattcagg ccaacctgaa actccctgac cgcccggact gccccggcta
    10321 ctccttcccg tgggtgggcg gcgaagccac gcatgtggtt attcccaacg aggtcatgga
    10381 acaagattgc ctgctggcat acgacggcga aacctatttt gaaggctcgc tggttgaacc
    10441 gctttcctgc gtgattggcg cgttcaacgc caactatcat cttcaggaag gtagttataa
    10501 ccacacgatg gggattcgcc cgcaagggcg cacgctgatc ctcggcggca ccggaccaat
    10561 gggattgttg gcgattgatt atgcgctgca tggaccagtt aacccgtcgc tgctcgtcat
    10621 taccgatacc gacaacgaca aattgagtta tgcgcgcaag cactatccat cagaaccgca
    10681 aacactgatt cattatctca atgccaccga tgcagcattt gatacgttaa tggcgctgag
    10741 tggcggtcac ggcttcgatg atattttcgt ctttgtgcct aatgaaggac tggtgactct
    10801 cgcctcttcc ttgctggcga cagatggttg cctgaatttc ttcgccggac cgcaggataa
    10861 acatttcagc gcgccaatta atttctacga tgtgcattat gcatttaccc actacgtggg
    10921 cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc aaactgattg aagagaaaaa
    10981 agtgcaggcc gcaaaagtgg taacacatat tcttgggctg aatgccgcgg gcgaaaccac
    11041 gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg tataccggga aatacctgcc
    11101 gctgacgtca ctcacgcaga ttcaggatca agcactggcg gcgattctgg cgcgtcatca
    11161 ggggatctgg tccggtgagg cggagcaata tctgctcact catgcagagg caatttccca
    11221 tgattaatcg cgctacttaa ctctgcatgt cgctggctgg tcgccgcggc aacttcgcga
    11281 cctgatgtgc ggctgattgc tgaagcggcc caatgtgcac gagaaggata aaaaaacatc
    11341 agcgccttaa ggggcgctca aattggtggc aaagtacaca ttgtttatgc cggatgcgga
    11401 ataaagcctt attcggccta cataatctcg aaaattcaat atattgcagt tatctggtag
    11461 gcctggtaag cgtagcgcat caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg
    11521 caatacgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg tagcatgcct
    11581 tccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
    11641 tgccgggtaa agcctggctg gaacatgcac tgccgctaat tgctgaacag ttgcagggtc
    11701 gccgctcagc ggtgtttatc cctttcgctg gcgtaacgca gacctgggat gattacacag
    11761 cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
    11821 tcgatcccgt tgccgcgatt gaaaatgctg agatcgtgat tgtcgggggc gggaatactt
    11881 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc accaattact gacgtggtta
    11941 aacgtggtgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
    12001 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc
    12061 cgctgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc
    12121 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt attggtctac
    12181 cggaaggtaa ctggatcacg gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
    12241 cttatgtgtt taaggccggt gaagaagcgg tgccgctgga agcgggtcac cgtttttaag
    12301 tgaattgccg gatgcatgat ccggcatctt gttcatagtg cctgatgcga cgcttgacgc
    12361 gtcttatcag gcctacagga ttcacaatat taatactcgt cccgtccttc atcttcatcc
    12421 ggctgttcca gcacactgta agccaccgaa caaaacagcg agttcagacg ctgcatatct
    12481 cccagtaagc ctaaatgtag cgaactggtt tcaatgctct gcacgttttg ctgatgcagg
    12541 cgatcgacgt gggcgtggga atagcggcga ttaagaatgc gaaagcgatg cttgctgcgg
    12601 cgcaaacgac gagcgctggt gacatcgcca gagaagaaca ccgacattgc cagctttaaa
    12661 ttactgagca attgctcata gagcgcatcc agttccttca acccatcaag cgaaaatgcc
    12721 cgccgtgctg ccagcgattt atcagcaatt tcgctgccca tgcgctcgac gatatcggaa
    12781 gcctgttcaa ggttgagcga catttcaata atctccgccc agcgacgcga ctcttcttct
    12841 gccagttcct cttttggcat tcgcgccaga tacagcttaa tggcggtgta gagaacgttg
    12901 atatcatctg ccagcttacg cagctctttc tcctgccgtg gctcgccgtg catcacttta
    12961 ttcagccctt ccatcatctg ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg
    13021 ttcgccagag caagcgtggg cgtatccagc gcgctgacat ccagatgttt aggccgtagc
    13081 tgggtatcca gttccggttc atcgcgaata atcgttttgc aaaaccgtgc catcgggtca
    13141 acaaatggca gcatgaccag gcaacgtaca aggttgtaga agacgtggaa atagatcacc
    13201 agttccgctt ttggcaatga caacttcccc attgtctctg ccagcaaatg gacaaacggc
    13261 aggataatca ggctacccac cagcttaaac agcaaactac ccagcgcgac acggcgggct
    13321 gcggcattgg cggcactgtt gttgagcatc gccagcaggc cggaaccgag gttagcacca
    13381 atcaccagac agagcgccac ggggaaggag ataatgcctg cggcggtcag agtcgcggtc
    13441 agcagtacag cagcaaggct ggagtagctg ataatggcga acatcgcgcc aatcagcgca
    13501 tccagcagaa tatcgccggt cagcgaggca aagatcacct gaacgccgtt tgcctgggtg
    13561 atcggcgtta cggcctgcac aatcaactcc agcgccagca aaatcagccc aagaccaata
    13621 ccgacgcggc ccagttgccc ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg
    13681 ataaaaataa gtaacggtga cagccaggat aagtcgaagg tgagaatacg cgccattagc
    13741 gccgtcccga catctgcacc cagcacaatc accagagccg gtgcgagggc taccagatcc
    13801 tgagcgacga acgaggtcac cagcatggtg gtggcattac tgctctgtac cagtgcggta
    13861 acgccgatcc ccgcgcaaaa ggcgagcggc tttttttcga cgctccggct aaggacagta
    13921 cgcaaacgcg cgccgaagac gcgcattacg ccggttcgaa caatatgagt cccccagacc
    13981 agcagggcga cggcagaaag caggtgaagc agcgttaaca cgagttcagg ttctccttat
    14041 cgttatatgt tcctgaagca attgaatgac caaaggaccg gaaacacgct gactggcatc
    14101 tttactgtgc cttttgacat tcgtttttcc ggctaagatt attccagcct gtttccagta
    14161 taagtgtttc atattaataa agagacaggg cactcattga gcgccctgtt tgttgtaagg
    14221 aaaagtgaca gatttgtgaa tcagtccgcg tcatacccca gattcggtgc cagccagcgc
    14281 tcaacatcgg agacgctcat acctttacgg cgggcatagt cttcaacctg atcgcgctga
    14341 atttgcgcca ccgcgtagta cttgctgtcc gggtggctga aataccaacc cgaaaccgat
    14401 gcaccaggcc acatggcgaa agattctgtg agtttcatgc cagtatgttt ttccacttcc
    14461 agcagctccc agatggtggc tttttccgta tgttccgggc aggccgggta gcccggtgcc
    14521 ggacggatgc cctggtagtt ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc
    14581 gcatagcccc agtagacttt acgcacacgc tcatggagat actccgcaaa ggcttcggct
    14641 aaacggtcgg caagcgcttt caccatgatt ttgttgtaat catcgtgctg cgcttcaaag
    14701 gcatcagcca gtgcgtcctc ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct
    14761 gctttaccag aaagcttcgg cgcaacgaag tcagcgagac agtagttagc gaatcccgtt
    14821 ttttcggtct gttgacgcag atggtggctg acgttgatca catgggtgcg cgtttcgtca
    14881 cggtagattt caatgtcatc gcccacacgg tttgccggga acaagcccac cacgccacgc
    14941 ggattcagcg ttttctcggc gcttaattta tccagcatgt cgttggcgtc tttaaacagc
    15001 cgctgcgcct caacgcccac cacttcatct tccagaatgc gcggatactt cccggccagc
    15061 gaccaggtca taaagaacgg tgtccagtcg atgtaattac gcagagtttc gatgctggct
    15121 tcaacttcct gcacgccgag acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa
    15181 gcgaagtcgt tatcgcgcgc cgcttccagc gtgaccggtg gtgtgcgcgg tttcttgcgc
    15241 ccgtgctgaa tacgtacggt ttcatactcc ttgcgggtac gagcgacaaa atcatcgcgc
    15301 tgagtatcgg aaagcagcgc cgccaccaca ccaacggtgc gcgaggcatt ctgcacatac
    15361 accgtcgggc cgctgtagtt ctgctcaatt ttcaccgccg tgtgcgcttt tgaggtcgtc
    15421 gcgccaccga tcagtaacgg aatggtgaag ccctgacgct ccatctcttt cgccacgtta
    15481 accatctcgt ccagcgacgg cgtgataagc cccgaaagtc caatcagatc agcattcact
    15541 tctttagcgg tacggagaat tttttccgca ggcaccataa cgccgagatc gacaatttcg
    15601 tagttgttac attgcagcac cacaccaacg atatttttac cgatgtcgtg gacgtcgccc
    15661 ttcacggtgg cgatcaccat cttgccgttg gttttgccct gctctttgct ggcttcaata
    15721 aacggttcga ggtaggccac tgcctgtttc atgacgcgcg ccgatttgac cacctgtggc
    15781 aggaacattt tcccctcgcc aaacagatcg ccgaccacgt tcatgccgtc catcaacggg
    15841 ccttcaatca cttcaatcgg gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg
    15901 ataaactcag taatgccttt gaccagcgag tattccagac gtttattcac ttcccacgag
    15961 cgccactccg cctgctgggc gttagcggtg tcgtcggttt tgctgccgcg atatttctcg
    16021 gcaagctcca gtaaacgttc agtgccatca tcgcgacgat taagaatcac atcttccacc
    16081 gcgtcgcgca gttcagcggg caggtcgtcg taaatcgcca gttgcccggc gttgacgatc
    16141 cccatatcca tgccattgcg aatagcgtag tagaggaaca ccgcgtgaat ggcttcgcgc
    16201 accggttcgt tgccacggaa tgagaaagaa acgttagata cgccgccgga aatcagcgcg
    16261 tgcggcagtt cgcgtttgat gtcttcgcag gcgccgataa agtcctgcgc atagttgttg
    16321 tgctcttcaa tgccggtggc gaccgcgaag atgttcgggt cgaagatgat atcttctggc
    16381 gggaagccaa cctcttcggt gaggattttg tacgcccgac ggcaaatctc gattttccgt
    16441 gcgcgggtat cggcctgtcc ctgttcgtca aaggccatta ccaccaccgc tgcaccgtaa
    16501 cgacgcaaca gtttcgcatg atggataaag gcgtcgacgc cctctttcat tgagatagag
    16561 ttaacaatgc ctttgccctg aatacacttc agcccctttt cgatgacctc ccattttgag
    16621 gagtcgatca taatcggcac gcgagcgata tccggttcac cggcaatcag attgagaaaa
    16681 cgcaccatcg ccgcttcggc gtcgagcatc ccttcatcca tattgatatc gataatctgc
    16741 gcgccgtttt ccacctgctg gcgggccaca tccagtgcct cgctgtattt ctcttctttg
    16801 atcaggcgct tgaacttagc ggaaccggtg acgttggtgc gttcacccac gttcacaaac
    16861 aggctatctt cgccaatgtt cagcggctcc aggccggaca aacggcaggc tacggggatt
    16921 tccggcagtt tgcgcggcgc taatccttct actgcacgac tcatcgctgc aatatgttgt
    16981 ggcgtggtgc cacagcagcc gccgacgata ttgagaaagc ccgcttccgc ccattcacgt
    17041 atttgttttg ccatcgtgtc ggcgtcgaga tcgtactcac caaaggcgtt gggtagcccg
    17101 gcgttcgggt gcgcggtgac gtagcattcc gcaatccgtg acagctcctg cacgtactgg
    17161 cgcagttcat cgggccccag cgcacagttc aggccaaagg tcagcgcttc ggcgtggcgc
    17221 aatgagttgt aaaatgcttc ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg
    17281 atggtgccag agatcataat cggcagctca acgcccagcg tttcaaactc cgtttttacc
    17341 gcaaataccg ccgctttggc gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc
    17401 gcaccacctt ccaccagcgc tttggtggac tctcgataag ccgccaccag cccgtcaaaa
    17461 gtgatattac gaaatgccgg atcgttgacg tccggagaaa tagacgccgt gcggttggtt
    17521 gggccgagaa caccggcaac gtagcgcggt ttctccggcg tgcgcgcggt ccactcgtca
    17581 gcacaggcgc gcgccagttt cgccgccgca aagttgattt ccgccgacag ggattccatc
    17641 tggtaatccg ccatcgcaat ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg
    17701 cccgcttcaa agtaggcgtt gtggatagcg gcgatcactt ccggtttact gagtaccagc
    17761 aggtcgttgt tgcctttgag gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg
    17821 gcttcgttca gtcgatagct ctggatcatg gtgcccatac cgccgtccag caccagaata
    17881 cgttcattta actgcgcacg cagttgttcc actttgctgc tcacacttgc tcccgacacg
    17941 ctcaacccag atttaataaa aattcaacaa accatactgg cataaacgca tctgtggtaa
    18001 aagcgaccac cacgcaacat gagatttgtt caacattaac tcatcggatc agttcagcaa
    18061 ctattgcatt aactaacaat aaaaatgaaa atgatttcca cgatacagaa aaaggagact
    18121 gtcatggtcg cacccattcc cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca
    18181 gcgactggac aggttcagtc tttaacgcgt ggcctgaaat tactggagtg gattgccgaa
    18241 tccaatggca gtgtggcact cacggaactg gcgcaacaag ccgggttacc caattccacg
    18301 acccaccgcc tgctaaccac gatgcaacag cagggtttcg tgcgtcaggt tggcgaactg
    18361 ggacactggg caatcggcgc acatgccttt atggtcggca gcagctttct ccagagccgt
    18421 aatttgttag cgattgttca ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg
    18481 gtcaatatgg cggtgcttga tcaaagcgat cacgaagcga ttattatcga tcaggtacag
    18541 tgtactcacc tgatgcgtat gtccgcacct atcggcggta aattgccgat gcacgcttcc
    18601 ggtgcaggta aagccttttt agcccaactg agcgaagaac aggtgacgaa gctgctgcac
    18661 cgcaaagggt tacatgccta tacccacgca acgctggtgt ctcctgtgca tttaaaagaa
    18721 gatctcgccc aaacgcgcaa acggggctat tcatttgacg atgaggaaca tgcactgggg
    18781 ctacgttgcc ttgcagcgtg tattttcgat gagcaccgtg aaccgtttgc cgcaatttct
    18841 atttccggac cgatttcacg tattaccgat gaccgcgtga ccgagtttgg cgcgatggtg
    18901 attaaagcgg cgaaggaagt gacgctggcg tacggtggaa tgcgctgact ttttctggcg
    18961 ggcagaggca atattctgcc catcatacct gagtggcaat agaataaggg tgtctgttaa
    19021 tcgcattgac gccaaaataa cttaatgtca tacacttcac acacttttat taactcagca
    19081 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc at
//
LOCUS       DAZPJP010000067        18567 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPJP010000067 DAZPJP010000000
VERSION     DAZPJP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.067"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1215
                     /locus_tag="ACHU9J_002139"
     CDS             <1..1215
                     /locus_tag="ACHU9J_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1905669.1"
                     /translation="HHQIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLMLHL
                     TAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLS
                     QYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPVAM
                     MIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYA
                     NHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGV
                     KSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQ
                     KKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKG
                     KRQEWGILCNND"
     gene            1188..1817
                     /locus_tag="ACHU9J_002140"
     CDS             1188..1817
                     /locus_tag="ACHU9J_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1905670.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1818..2978)
                     /gene="ybdL"
                     /locus_tag="ACHU9J_002141"
     CDS             complement(1818..2978)
                     /gene="ybdL"
                     /locus_tag="ACHU9J_002141"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1905671.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3087..4175
                     /gene="hcxA"
                     /locus_tag="ACHU9J_002142"
     CDS             3087..4175
                     /gene="hcxA"
                     /locus_tag="ACHU9J_002142"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1905672.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4185..4382)
                     /locus_tag="ACHU9J_002143"
     CDS             complement(4185..4382)
                     /locus_tag="ACHU9J_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1905673.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4564..6669)
                     /gene="cstA"
                     /locus_tag="ACHU9J_002144"
     CDS             complement(4564..6669)
                     /gene="cstA"
                     /locus_tag="ACHU9J_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1905674.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6850..7263)
                     /gene="entH"
                     /locus_tag="ACHU9J_002145"
     CDS             complement(6850..7263)
                     /gene="entH"
                     /locus_tag="ACHU9J_002145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1905675.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7266..8012)
                     /gene="entA"
                     /locus_tag="ACHU9J_002146"
     CDS             complement(7266..8012)
                     /gene="entA"
                     /locus_tag="ACHU9J_002146"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1905676.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8012..8869)
                     /gene="entB"
                     /locus_tag="ACHU9J_002147"
     CDS             complement(8012..8869)
                     /gene="entB"
                     /locus_tag="ACHU9J_002147"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1905677.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8883..10493)
                     /gene="entE"
                     /locus_tag="ACHU9J_002148"
     CDS             complement(8883..10493)
                     /gene="entE"
                     /locus_tag="ACHU9J_002148"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1905678.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10503..11678)
                     /gene="entC"
                     /locus_tag="ACHU9J_002149"
     CDS             complement(10503..11678)
                     /gene="entC"
                     /locus_tag="ACHU9J_002149"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1905679.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12053..13018
                     /gene="fepB"
                     /locus_tag="ACHU9J_002150"
     CDS             12053..13018
                     /gene="fepB"
                     /locus_tag="ACHU9J_002150"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1905680.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13099..14349)
                     /gene="entS"
                     /locus_tag="ACHU9J_002151"
     CDS             complement(13099..14349)
                     /gene="entS"
                     /locus_tag="ACHU9J_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1905681.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14460..15464
                     /gene="fepD"
                     /locus_tag="ACHU9J_002152"
     CDS             14460..15464
                     /gene="fepD"
                     /locus_tag="ACHU9J_002152"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1905682.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15461..16453
                     /gene="fepG"
                     /locus_tag="ACHU9J_002153"
     CDS             15461..16453
                     /gene="fepG"
                     /locus_tag="ACHU9J_002153"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1905683.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16450..17265
                     /gene="fepC"
                     /locus_tag="ACHU9J_002154"
     CDS             16450..17265
                     /gene="fepC"
                     /locus_tag="ACHU9J_002154"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1905684.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17262..18395)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU9J_002155"
     CDS             complement(17262..18395)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHU9J_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1905685.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4405 a         5245 c         4788 g         4129 t
ORIGIN      
        1 catcaccaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg tattacctgg
       61 acactcaaca ccctaccccg cgtttgtgtc tcattttctg gcggcaaaaa ctctggttta
      121 atgctgcatc tgacagccga acttgcccga caaatgggca aaaaaatctg cgttttgttt
      181 atcgactggg aggcgcaatt ctcttgcacc attaactatg ttcagtccct gcgcgagttg
      241 tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac gcaaaattcc
      301 ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg ggtgcgtcag
      361 cccccgcaag atgcgataac cgatcctgac tttttctgct tttaccagcc aggcatgacc
      421 ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc ggtggcgatg
      481 atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc cagtttaaat
      541 aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg tcatagctgg
      601 tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta tgcaaatcat
      661 caaaatctct gcaacccact gtataaccta atgtatcagg caggcgttcc tctgcgtcat
      721 atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct ctatcacgtt
      781 atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa aagtggcggc
      841 atttacgccg gacatgataa tcatttctat gggcatcgga aaatcctcaa gccagaacat
      901 ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaaa aacagctgag
      961 cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg catcgaggtt
     1021 ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg gcggatatgc
     1081 aaagttttac tcaataacga ttactggtgt cgggcattat catttagtcc tacgaaagcg
     1141 aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg ggggatacta
     1201 tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg gaagatgatc
     1261 gcatcaaagc gattaatgaa atccgcatgg cgatccatca ggttagcccc tttcgtgaag
     1321 agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat gattacaacc
     1381 caaacaacgt ggcaccaccc gaaaaaaaac tgctgcaaaa atccattgaa atagatggtt
     1441 ttacccaacc cattgtggtc acacatacgg ataaaaatgc catggaaatc gtggatggtt
     1501 ttcaccgcca tgaaattggt aaaggctcat catcattaaa attacgctta aaaggttatt
     1561 tgccggtgat ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact attcgtcata
     1621 atcgcgctcg tggacgccat caaatcaccg ccatgtcgga aatcgtccgt gaacttagcc
     1681 agttgggatg ggacgataat aaaattggca aagagctggg aatggacagc gacgaagtgc
     1741 tgcgcctgaa gcaaattaac ggcctgcaag agctgtttgc cgaccgtcaa tattcccgcg
     1801 cctggacggt taaataacta aagctggcgc aggcgttcag ctgctgccag caacgtcgat
     1861 tccttcttgg caaaacagag acgaatcagt ttatgtggga agggatcggc gcaaaacacc
     1921 gacagcggaa tcgccgctac gccgtgctcc tgcgtcagcc actggcaaaa ctcaacatca
     1981 tccagggtag aaaccgcgct gtaatccacc agcaaaaagt atgtaccttc acacggtaaa
     2041 atctccagcc ggctttcatt taaggcattt accagaatat cgcgcttctg gcgataaaag
     2101 tccggtaacg caagataatg ctcaggttct gcacgtagca tatcagcaag cgccagctgt
     2161 gccggggtat tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc ggcgctgatg
     2221 ggcgctggcg caacacaata acccactttc cagccggtca tatgataggt cttgccaaat
     2281 gaagaaaccg ccactgcccg ctcacgcagc tgcgaatgcg ccagcacact ggcatggccc
     2341 tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat ctcgtgcccg
     2401 gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc agacagttgc actggagtta
     2461 tgcggagtgt tgaggatcac cagtctggtg cgctcgctta ataatgcggc aaattcctgc
     2521 cagtcaacgc gaaaatgcgg tggttgcagt gccatacgct tcactattcc cccagaaagc
     2581 gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac aaatcacttc atcgccattg
     2641 cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt tacggtgata
     2701 tcgctatcgg catctggttg atagccatac aaacgttccg ttttctgagc aatcgcctcg
     2761 cgcaaggcct gcacgccggt catgggcgcg tattggtttg ccccctgggc aacgtggtac
     2821 gccagccgct cctgtaaata gcgcggacca tcaaaatcag gaaagccttg cgacaggtta
     2881 atcgcctggt gttgctgcgc cagcgcgctc atctgggtga aaatagtggt gccaagttgt
     2941 ggaagtttgc tttgtggaat cagagggtta tttgtcattt ttattgtacc tgacgaagtt
     3001 gcgtgttgaa ggcactataa cacgatgcta gtatttggca atcaagacgt ttagatgtct
     3061 aaataaaaca ataaggacaa cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc
     3121 cggctaacta cttttcacat ccaggaagtt tcaatcacct gaacgatttt ttcactgatg
     3181 aacaactttc tcgcgcggtg tggatctacg gcaaacgcgc cattgctgcg gcgcaaacca
     3241 aacttccgcc agcgtttgga cttccagggg caaagcatat tttgtttcgt ggtcattgca
     3301 gcgaaagcga tgtacagcaa ctggcggctg agtccggtga cgatcgcagc gtggtgattg
     3361 gcgtcggcgg cggcgcactg ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc
     3421 catttgttgc cgttccgacg atcgccgcta cctgcgccgc ctggacaccg ctctccgtct
     3481 ggtataacga tgccggacag gcgctgcatt atgagatttt cgacgacgcc aattttatgg
     3541 tgctggtgga accagagatt atcctcaacg cgccgcaaca atatctgctg gcggggatcg
     3601 gtgacacgct ggcgaaatgg tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc
     3661 cgctaaccgt gcgactgggg atcaataatg cgcaggccat tcgcgacgta ttgttaaaca
     3721 gtagcgaaca ggcgctgagc gatcagcaaa atcaacagtt aacgcaatca ttttgcgatg
     3781 tggtggatgc tattattgct ggtggtggga tggtcggtgg tctgggcgat cgttttacgc
     3841 gtgtggcggc ggctcatgcc gtgcataacg gtctgactgt cctgccgcaa accgagaaat
     3901 ttctccacgg caccaaagtc gcttatggca ttctggtaca aagcgccttg ctaggtcagg
     3961 atgatgtgct ggcgcaatta accggggcgt atcagcgttt tcatctgccg accacgctgg
     4021 cggagctgga aatggatatc aataatcatg cggagatcga caaagtgatt acccacaccc
     4081 tgcgtccggt ggaatacatt cattacctgc cagtcacgct gacaccagat acgttgcgtg
     4141 cagcgttcga aaaagtggaa tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg
     4201 ccgccttttc caccgtagcg cgcgtcctgc cgctcacgga aaaactcttc gtaggtcatc
     4261 ggcgtttgat cgggatggtt aacccgcata tgttcgacat agttgtcgta atcaggcata
     4321 ccaatcatca gcttcgccgc ctgacctaaa tattttccgg cttttgccag tgaatcaaac
     4381 atcttggcct ccgctaacca tgttgctgaa agagtgccgg atgcggcgtg aacgccttat
     4441 ccggcctaca aatgctatca acgcgctcgg tcagtcccct cgccccatcg gggagagggt
     4501 tagggtgagg aggaaccctc ggcactatgc caacattcgc caacatcccc cctcactctg
     4561 actttagtgt gcgccttttg cctgcgccac gatctcctcg acattttccg gcattggctc
     4621 atacggcgtt tctttcgccg ttggcttcgg atctttcaat gccgccagtg ccgacttaat
     4681 cgagaacagc gccagaacca ccacgaccac cataaagaag atggttaacc cggcatccag
     4741 acggttgttg aacaccagtt gcgccagctg tgactcagta tactgcgacg gaatattgcc
     4801 gctgtcgatc attgcctgga acttattagc aatggccagg aagccgactt tcgcatccgg
     4861 gctaaacgct ttctgccagc ctgcggtcag ggtacaaatc agcagccagg ccgttggtac
     4921 cagcgccacc caggcgtaac gttgacgttt catcttgaac aacaccacgg cacagagcat
     4981 cagcgccatc cctgccagca tctggttggc aataccaaac agcggccaca gagtgttaat
     5041 gccgcccagc ggatcgacca cgccctgatg gaggaagtag ccccacgcca gtacgcacag
     5101 cgctgttgcc agcaggttag caggcagtga atcggtccgc ttcaggccag gagacaccac
     5161 gcccagcaga tcctgcaaca taaagcgcgc agcacgcgta cccgcatcta ccgccgtcag
     5221 aataaacaga gcttcaaaca gaatggcgaa gtgataccag aacgccacat ccatcatacc
     5281 acccagcgca ccgtgcagga tgtaagccat ccccaccgcc agcgtcggcg caccgcccgc
     5341 acgggaaatg atcgactgtt cacccacttc gctggcaatc tggttgagcg tatccggagt
     5401 aatcgcaaag ccccagctac tcaccacctg cgcggcagaa gcgaccacat ccgccgtccc
     5461 tgccggagcc agcaccgcca tcgggctgtt catggcgaag tacacgcccg gatcgatgat
     5521 acaggcggaa accagtgcca taatcgccac gaaggattcc attaacattc caccgtagcc
     5581 gataaagcac gcctgccctt cgttcgccag catcttcggc gtggtgccgg aagagatcag
     5641 cgcatggaag cctgacaccg cgccacaggc gatggtgata aacaggaacg ggaacagatt
     5701 accggtccat accgggccag tgccatcgac aaatttggtc agcgcaggca tggtcagcgt
     5761 cggacgcata atcaaaatgc ctaccgccag accaacgata gtcccgattt tcaggaaggt
     5821 agagaggtag tcacgcgggg ccagcagtaa ccacaccggc agcaccgccg ccacaaaacc
     5881 gtagcccacc agcatccagg tcagctgcac gccggtaaag tcaaagtacg gtgcccaggt
     5941 cgggctttct gccacccagc cgccagagat aatggcaaaa ataaggaaca ccaggccaat
     6001 cactgacact tcgccaatac gccccggacg cagatagcgc aggtagattc ccataaaaat
     6061 cgccagcgga atggtgaacg caacggtata cgttccccac gggctatgag tcagggcttt
     6121 caccacgatc atcgccagta ctgcaaggat aatgaccatg atcataaagc aggctaccag
     6181 cgcaatcacc ccggcagttg gccccatctc ttctttgacc agctcaccca gcgagcgacc
     6241 gtcacggcgc gtagaaacaa acagcaccat gaaatcctgc accgcaccgg cgagaaccac
     6301 cccggcgagc agccagatca tccccggcag gtagcccatt tgcgctgcca gcaccggccc
     6361 caccagcggc cctgctccgg caatggccgc aaaatggtga ccgaacagca ctttcttgtc
     6421 cgtcggcaca tagtccagcc cgtcgttatg gcgcaccgct ggcgtcatac gcgtcgggtc
     6481 aaccgccagc acatttttgg cgatatacag cccataaaaa cggtaagcga tcagatagat
     6541 acagaccgac gccaccacaa tccacagcgc gttgatctgt tccccacgat ttaaagcaat
     6601 gtatcccaga gcaaatgctc ccattacaga gagcactgtc cagacgaggt atttccctga
     6661 tttgttcata gttgttatcc gtgtgcgtgt ccatagagat gttacatttt gtttctataa
     6721 catctttgta cgatttaaca acacggtaac catgtaaatc ggagttgaaa ccaatattta
     6781 acttaacaat gttaactcga tcactccgtt aaccgagttt tcctgcaatc tcatttaatt
     6841 ctgtcgggct catcccaaaa ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg
     6901 ttcatcgaaa acgatgattt cccagctctg attttgccgc ccaagatgca gcggctggca
     6961 gacgccgcgt accttgcctt cagacaccgg gcgatgatgg gtggcgttaa gttccgtacc
     7021 caccacacac tgcccgtcac gggtcatcat aaatccggcc atcgatccca gcgtttcagc
     7081 cagcgccgcc gacgcgccgc catgcagcag gccaaacggc tgatgagtac gggtatcaac
     7141 cggcatttcg gcttccagca catcatctcc cagacgggta tacacaattc ccagatgcgc
     7201 caccattgtg ttatcgctgg tggcgttcag ttcgtcgagc gttaaatggc gtttccagat
     7261 catgcttatg cccccagcgt tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg
     7321 ctggcgaggt cagaggcgag gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt
     7381 ttccccagcg gaatgccgag tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg
     7441 gcgtcatcgc tcacccacag cgtgcgttgc atatcggtgt cggtggagcc aggcgaaacc
     7501 acattacagc gcacgccgct aaccgccagt tccagcccga cgctcaacgc caggcttttc
     7561 agcgccgctt tcgatgcgcc ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg
     7621 gacgccacag tgacaatcgc cccgccccgc tgacggcgaa actggttcat ggtttgctga
     7681 aacaggttaa acgcaccgcc gacgttaacc gcaaaagtct gctgccagtc ctctttactg
     7741 agctgatcgg tcgcgcccat gcgcaaaatt cccgccgcat tgaccagcac gtccagtcgc
     7801 tccgtttcag ctaacagtcg ctgacatacc tgcgcgacct gcgcagcatc ggcaacatcc
     7861 atcacttcgg tcgcaaaggg atattgctcc tgagcgaacg cttgatcaaa accggtaact
     7921 ttcgctcccg cttcaacaaa cgccagcgcc gtggcgtagc cgataccttt acctgcgccg
     7981 gttacccaga catttttacc gctgaaatcc attatttcac ctcgcgggag agtagcttcc
     8041 accaggcgtc gatggtcggg tttttcgcca gcatgacaaa gtcgatatcg ccatgcactt
     8101 tgcgccagcg cgccgccagc gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt
     8161 tgtcgtcatc gaacggttca tcggactcgt ccagcaacgg caggataacc tcacgcagcg
     8221 ccgctttcct ggcggggaca ggtgctggca gtaatccttc agtcatcacc acccggccag
     8281 accgcccggc cacatatttc agcgacatca aatgctcgtc acggctgaaa tcggccagcg
     8341 catccgccac cataaacggt ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac
     8401 agccaatgtg ggcatatacc ccggtaataa tcagctggtt acgtccactc tctttgagca
     8461 tttgctccag cggagaacga tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt
     8521 cggcatctgg cgtcaggcga tccaccacct tttgctgttc cggcgaacgg gtcaggcccg
     8581 gcccccacat atcattcaac agcgcccgat cttcatcgct ctgctctttc ggctgggcgg
     8641 tgtaataaac cgggatattg tgctgtttgc agtagtcgcg cagcgcagca atattcgcga
     8701 tcacctgctc catcatcggg cagttctcgc cccagaagct gacaaaatag tcctgcatat
     8761 catggattaa caacgcggca cgttgcggtt caaaggccca gtcaacttta ttctgcggaa
     8821 tatcgtgaga ctccggcagt gcgtaagcct gtaattttgg aatagccatc gtgttctctc
     8881 cttcaggctg atgcgcgtga cgccagccac tgacgtaatt gttttttatc gactttcccg
     8941 accgccgtca gcggaagtga atccacacac tccacgcgat ccggtaattt aaattcggca
     9001 ataccctgtt cacgcaggaa acgacgcacc tgcaccgcgc gcagcggctc ttttaccacc
     9061 agataagcac agcttttttc gcccatcagc tcatcttcca tgctcaccag tgcggcatag
     9121 atcaccgccg gatggcgcag cagcaggttt tcgatctctt cggcagcgat cttctcgccg
     9181 ccacggttga tctgatcttt ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata
     9241 gagatcagat cgccggaaca gtaaaaaccg ttggcatcaa aggcactggc attgtgctgc
     9301 ggacttttgt aataaccgcg gaaggtgtac ggcccgcgcg tcatcaggcg tccaacttcc
     9361 ccttgcggca gtggatttcc ttcagcatca gcaacccata cttcatcgtc cggacacatt
     9421 gggtaaccct gggtatggat aattttctcc gcgctatcat caagtcgggt gtagttcacc
     9481 agcccttccg ccatgccaaa cacctgctgc aactggcagc caatctcagc ggaaatacgc
     9541 gccgcaaggg tggcagaaag acgtgcgccg ccgacctgta acagtttcag cgaggcaagc
     9601 tgcgcccggc tttcgccttc agccagcgcc tgcaaccaca ggctgactgc cggcggcacc
     9661 agcgcggtaa cgttaacctg atgttttgca atcaacggga agcaaagcgt ggcgctggga
     9721 tcggccgcca gaacaaccgt tcctccggca agaaagacgc ccagcgatcc tggcgaactc
     9781 atagcgtagt tatgagccgc cgggatcgcg cacaggtagc gtgtctgttg tgtgaactga
     9841 caaatctcga cgctacgacg cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc
     9901 ttcggtgtac cggtggtgcc gccggaaagc tggaaatagg ccacttcatc agcaggtgat
     9961 ggcgtagcag taaaaccgtc tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg
    10021 ctgtcgttga gcagttgcac cacgcgaatg gaggaatgtt ctgtgacgaa agtattgagg
    10081 aaatcatccc cgctaaacag cgcatgttgg cgatcggcaa tcagtaacgc cggttcaatc
    10141 tggctggcat aggcgttcag ttcactacgc tgatggctga acaacgccag caccggcgca
    10201 acgcccagtt tcagcagtgc gaaaaaggta atgtaaagtt cagcgacgtt acccagttgt
    10261 accagcgcgg tttcaccggg tttaatgccc tgacggcgta aactacacgc gaggttatcc
    10321 gccgcctgat tcagctcccg gtaactcaac tgtcgctcgc cgtcgataac cgcgatgctg
    10381 tcactcgcag cgtggcgagt cagaatgtcg gtcagcggca aatcctgcca gtagcctttt
    10441 tcccgatagc gacgggcaaa ctcttccggc cagcgggtga atggaatgct catcctcgct
    10501 ccttaatgca atccaaaaac gttcaacatg gtagaaagtt tgacgcctgt ttcgcgccac
    10561 tcacccaacg gtgacgacgc aggcacaatc cccgctccgg caaacagacg cacctgattt
    10621 tcccgcagct tcgcgcagcg gatggtcacc acccattcgc cgttaccttc gctgtcacac
    10681 caacccacaa tgccgccaaa cagttcgcgg tcaaacggct ccagttcagc aataacctgg
    10741 gtcgcggcct gatgcgggaa accgctcagc gcgggggtcg gatgcagcag acaggccaga
    10801 gtcagtgcgt tttcttgcga attcgcttta ccttcaaagg gagttgcgag atgccacagc
    10861 gtcggcgtgg taatcaattg tggagaggag ggaacgtgta actcactact gcgttcgcgc
    10921 agtacctctt tcatcgcctg agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc
    10981 agcagacgat tacccgcttc gcgatcgagc acttcatccg gctgacgacg cgcggaaccg
    11041 gctaacggaa tggagctaaa acgctcgccg tctttacgta gcagcagttc cgggctggcc
    11101 cccagcagga cgccaccatc agccagcggg acatggaagt tgtaactaac cgggttttgc
    11161 gcaatcaacc gttccagcaa tacgccacta tcaatggcgg cgtcagtggt gatatcaatc
    11221 aatcgtgaca acaccacttt gtcgacctgc ggcgtggcgg taagtgcggc ggcgcgggca
    11281 accatctgtt caaacgtggt ttgttcagga attgcctggc gttccaccac gttcagcgac
    11341 tggctgcggg taaaacagtg ggctgaggtc tgtttttcct gacgggagaa cgactgccag
    11401 gattcgggaa tatacagcga cgaaggctga cgtggatcga agggaatcgc cccgaccatc
    11461 accggatttt tgatgccctg cgctttggca tcggcaaaca gcgcggcgag tttttgctgg
    11521 aagggactgt cgggcgaatc cccgttcaca gccggttcat cgaagcgggc gaaacatcct
    11581 gacgtcgtaa aactgcggta cggcgacata aagaaaaagc gattgggcgc aagtgttgcc
    11641 atggtctgct gtacttcctc agccagtgac gtatccatat catcctccac aaaatgataa
    11701 aggctttaat aatgattatc atttatattt tcggtcgcta acctaaaagc aaacgcgcac
    11761 tatgtcaact cttgaggtaa cgcaatctgc aactcggggt tgcatctggt tgtggttaca
    11821 atgaaaatga gaagcattat tgatgaattc gcataagcgc aatgtgatgt cctgcgccgt
    11881 tttgccccct ctcccttcca gggtgagggc tggggtgagg gttaatgttc gcaccagtgc
    11941 tggctgttcc cctcacccta accctctccc caaaggggcg aggggacgga ttgtgcgctt
    12001 tgtcgaattt gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact
    12061 cgccccgctc taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt
    12121 agttcaggcc gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga
    12181 aagccagccg cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat
    12241 tgatgctccg gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca
    12301 gggcttttta cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat
    12361 cggcgaaccg agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc
    12421 aaccggcggg gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt
    12481 aatcatcaat tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac
    12541 cgggcatgag aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc
    12601 gaaagagcaa atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc
    12661 acacagtgcc aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg
    12721 ctttacgctg gcgaagttgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca
    12781 tgacatcatt cagcttggtg gggaaaatct ggctgctggg ttaaacggtg aatcgctatt
    12841 cctgttcgcc ggtgatcaga aagacgccga tgcgatttat gccaatccac tgctcgcgca
    12901 cctgcctgca gtacaaaaca agcaggttta tacgctggga accgagacgt tccgcctgga
    12961 ttactacagc gccatgcaag tgctggacag acttaattca ctgttaaaaa aagattgata
    13021 aaacatacaa ttagagcact aataatcccc tcgccccttt ggggagaggg ttagggtgag
    13081 gggaaaatgc acctttagct aactgtcaga cgctgtcacc tgcggcggcg tctggcgaaa
    13141 acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc
    13201 gctcgcgctt gcggaagcaa ccggtgtcat catcgcgccc aaaccaccca acagcgccgc
    13261 gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa ccgttaatcc gccctaacat
    13321 cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa ccgcgctcag
    13381 ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag
    13441 accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcact
    13501 atgtgccagc ttcccgctgg ttaacgcacc aatagccgca ccgagcggga tcgccgcata
    13561 gagaaaacca atctgtgccg ccgacatctg ccagttgtca gccagcgccg gatacagtac
    13621 ccgcaccgcg ctcgccatcg tcaataaacc acccagcaac gcaatccctc ctaccagcgg
    13681 gctggcgagc agaaaacgaa atcctgccag taatgatttc aacggatgct cgcgcggctg
    13741 cggtggcggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa acgtgcccgc
    13801 cgccgccagc ccgtagttcc aggctacgcc gccggtcgcc agcaataaac cgccaatcat
    13861 gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa
    13921 gttttcacgc cctaccagtg caggtgttgc cgccagtagc gccgtaacac caagtgatgc
    13981 gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct ccggcagcag
    14041 cgcgttcagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    14101 ttttttgcgc tcatagcgat ccgccagcac accgccaacc atcaggccaa caaacatcgc
    14161 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14221 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    14281 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    14341 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    14401 gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta
    14461 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga
    14521 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgcg tcagtagtgc
    14581 tggaggcctt ctcaggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    14641 tgccgcgcac ccttgccggt ttacttgcag gcggtgcgct tgggcttgct ggggcgttaa
    14701 tgcaaaccct cacccgaaat ccacttgccg accccggctt gcttggcgtg aacgccggag
    14761 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcatccgcg caggaacaac
    14821 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt atcggcagtc
    14881 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcgg
    14941 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac gatcaactgc
    15001 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    15061 tcccggttct gatcgccgga gcaactgcgc tattactgag tcgcgcgctg aacagtttga
    15121 gcctcggcag tgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttgattg
    15181 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15241 ttatcggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt
    15301 cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc
    15361 gggtgattgt gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg
    15421 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggtgc atgatttacg tctctcgccg
    15481 attactcatc acctgtttgc tgctgatttc cgcctgtgtg gttgcaggta tctggggatt
    15541 acgcagcggt gccgtcacgc tggaaatctc gcaggtattc gccgcgttga tgggcgatgc
    15601 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    15661 gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt cagtcgctga tgcgtaaccc
    15721 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtggc
    15781 gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat
    15841 tgtcacttct ctgttggtct ggctgctcgc ctggcgtaac ggtatcgaca cctttcggtt
    15901 gattattatc ggcatcggcg ttcgcgccat gctggtggcc tttaatactt ggctgttgct
    15961 gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgccggat cgctcaatgg
    16021 cctgacgtgg gcaaaaactt cgccttccgc acccatcatc atattgatgc tcattgccgc
    16081 cgccttgctg gtgcggcgaa tgcgcttgct ggagatgggc gatgataccg cctgcgcgct
    16141 gggcgtcagc gtcgagcgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc
    16201 tgcggcaacg gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc
    16261 ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt gcggagcgct
    16321 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    16381 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    16441 tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac cctgggatat
    16501 ggcaaatata ccgttgcgga aaatctgact gtagaaattc ctgatggtca cttcacggca
    16561 attatcggac caaatggctg tggtaaatcc acgttactgc gtaccttaag ccgcctgatg
    16621 acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    16681 gaagttgcac gccggattgg tctgttggcg caaaatgcta ccacgccggg cgatatcacc
    16741 gtgcaggagc tggtggcgcg tggacgttat ccgcatcaac cgctgtttac ccgctggcgc
    16801 aaagaggatg aagaagcggt aacgaaagcg atgcaggcca cgagaataac tcatctggca
    16861 gatcaaagcg tggataccct ttctggcgga caacgccagc gagcgtggat cgcgatggtg
    16921 ctagcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct ggatatcagt
    16981 catcagattg atctactgga actgttaagc gaactgaacc gcgagaaagg ctataccctg
    17041 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    17101 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaactgatt
    17161 gagcgcattt atggtctgcg atgcatgatc attgacgatc cggtagccgg aacgccgctt
    17221 gtggtgccgc tcggacgaac ggcaccttca accgcaaata gttaaactaa gtggtctgcc
    17281 atcatggcat cctgttttct ggatgccatc gcatagcgca ataacacgct accacaagcc
    17341 accatcccgc cgatcagcgc ggaaaggatc acaataatcg ccttacccgg accgtctttt
    17401 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgaca
    17461 tttgcttttg ttaattgctc gacaagatac tgccgattac gtaattcacc gttcagttcc
    17521 gcaacgtcag taaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga
    17581 gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtatac gggcttttta
    17641 attcctgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    17701 tgatttttcg ttttaatgcg atcctgagcc agtttttctt tttcaaactg ggttttgatc
    17761 tccagtttat tacggacgtt ttctatcgac tctttcacca ccaacgcaga gatataatcg
    17821 atatacccgc tcaaaacggt ctgcgcctct ttactggttg gcgcggtaaa acttagcgtc
    17881 caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    17941 attttttcgc tcaatgcaac aatagcgcga tgcaaatcca gttcatcgat tttcgcctct
    18001 tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaacagac
    18061 tgaaacttct tgataaacag gttaaatgca ccgccacggt cgatattaac atcgagatca
    18121 agaacgcgga gtttggtaaa ggttttctcc aggtcctgcc actgaatggc ttcggcggga
    18181 gtaataacgg ctgagctggt ccatttttgc ggcaggatga aagagatcag taaacctgcg
    18241 caggcaaatg caaaaacgac cgccatgacc gtttttttgg cccgccataa aacttcgatt
    18301 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc agaaaaatga
    18361 gcgtcacttc cctgtttaat attcaatgat gacatggggt ataacctgaa aatgagtcca
    18421 ataggggaaa atctccacgg cgctcacttt accagttggt gagaattatc cgaagctgaa
    18481 gtttgtaaat tccttccact gaatgcaata ttcactctat ttaagagaaa tattcatcaa
    18541 acctataaat caaacaaata aaaaacc
//
LOCUS       DAZPJP010000068        18219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPJP010000068 DAZPJP010000000
VERSION     DAZPJP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.068"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            21..1097
                     /locus_tag="ACHU9J_002156"
     CDS             21..1097
                     /locus_tag="ACHU9J_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1905686.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1490..1831)
                     /locus_tag="ACHU9J_002157"
     CDS             complement(1490..1831)
                     /locus_tag="ACHU9J_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1905687.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1844..2716)
                     /gene="copD"
                     /locus_tag="ACHU9J_002158"
     CDS             complement(1844..2716)
                     /gene="copD"
                     /locus_tag="ACHU9J_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR1905688.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2720..3094)
                     /gene="yobA"
                     /locus_tag="ACHU9J_002159"
     CDS             complement(2720..3094)
                     /gene="yobA"
                     /locus_tag="ACHU9J_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1905689.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3233..3463
                     /gene="holE"
                     /locus_tag="ACHU9J_002160"
     CDS             3233..3463
                     /gene="holE"
                     /locus_tag="ACHU9J_002160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1905690.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3565..4221
                     /gene="yobB"
                     /locus_tag="ACHU9J_002161"
     CDS             3565..4221
                     /gene="yobB"
                     /locus_tag="ACHU9J_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1905691.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4245..4907
                     /gene="exoX"
                     /locus_tag="ACHU9J_002162"
     CDS             4245..4907
                     /gene="exoX"
                     /locus_tag="ACHU9J_002162"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1905692.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4904..6964)
                     /gene="ptrB"
                     /locus_tag="ACHU9J_002163"
     CDS             complement(4904..6964)
                     /gene="ptrB"
                     /locus_tag="ACHU9J_002163"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1905693.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYQHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKMTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7173..7832)
                     /locus_tag="ACHU9J_002164"
     CDS             complement(7173..7832)
                     /locus_tag="ACHU9J_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1905694.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8159..8515)
                     /gene="yebF"
                     /locus_tag="ACHU9J_002165"
     CDS             complement(8159..8515)
                     /gene="yebF"
                     /locus_tag="ACHU9J_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1905695.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8582..8872)
                     /gene="yebG"
                     /locus_tag="ACHU9J_002166"
     CDS             complement(8582..8872)
                     /gene="yebG"
                     /locus_tag="ACHU9J_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR1905696.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9006..10184
                     /gene="purT"
                     /locus_tag="ACHU9J_002167"
     CDS             9006..10184
                     /gene="purT"
                     /locus_tag="ACHU9J_002167"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1905697.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10240..10881)
                     /gene="kdgA"
                     /locus_tag="ACHU9J_002168"
                     /gene_synonym="eda"
     CDS             complement(10240..10881)
                     /gene="kdgA"
                     /locus_tag="ACHU9J_002168"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1905698.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10918..12729)
                     /gene="edd"
                     /locus_tag="ACHU9J_002169"
     CDS             complement(10918..12729)
                     /gene="edd"
                     /locus_tag="ACHU9J_002169"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1905699.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12964..14439)
                     /gene="zwf"
                     /locus_tag="ACHU9J_002170"
     CDS             complement(12964..14439)
                     /gene="zwf"
                     /locus_tag="ACHU9J_002170"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1905700.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14777..15646
                     /gene="hexR"
                     /locus_tag="ACHU9J_002171"
                     /gene_synonym="yebK"
     CDS             14777..15646
                     /gene="hexR"
                     /locus_tag="ACHU9J_002171"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1905701.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15774..17216
                     /gene="pyk"
                     /locus_tag="ACHU9J_002172"
     CDS             15774..17216
                     /gene="pyk"
                     /locus_tag="ACHU9J_002172"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1905702.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17276..18118)
                     /locus_tag="ACHU9J_002173"
     CDS             complement(17276..18118)
                     /locus_tag="ACHU9J_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR1905703.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4673 a         4573 c         4393 g         4580 t
ORIGIN      
        1 gttaatggag aagttaaaac atgcagcgga aaaaatacga tcccaatttg cctagaaact
       61 taacatatcg aaggagagac aaagcatatt actggcgcaa ccctctgacg aaagaagaat
      121 ttacactagg taaaatttca agaagagatg cagtaacgca ggcaattgaa gcaaatcatt
      181 atatatacaa aaactactct cccgctgcct taattgaaaa acttaaaggg ttcgactcat
      241 ttactatggc agactggatt gaacgttaca aaacgattct tataaggaga aaagtgtcca
      301 gaaatactta taaaattcgg gcaaatcaac tggagacaat aaaagaaaaa ttgggagaga
      361 ttttactgac agaaataacc actcgccata ttgccgagtt tcttgatttg tggattgaag
      421 gagggaaaaa cacaatggca ggatcaatgc gttctgtgtt gtctgatatg tttcgcgagg
      481 ccattgttga aggacgtata tctcaaaatc cagtcacgcc aacaagagca ccgaaaatag
      541 tagttacaag agaacgactg aaactaaaga tatacaactg catcagggag gcagcagatc
      601 aacttccggc atggttccca ttagctatgg atttagccct tgtaacagga caacgtcgcg
      661 aagacataac gaatatgcgg ttcagtgata tttatgatga tcgtctccac gtcaggcaaa
      721 ttaagacagg aatgatgatt gctatccccc tgtcactcag ccttcctgtc gctggtctac
      781 ggcttggtac agtagttgaa cgatgccgcc tggtaagccg gggagattat ctaatcagtg
      841 ccgggattag aaaaaacagc cctgacggca gcattcaccc ggatggcctg acaaaaaaat
      901 ttgtcgcagc cagaaaattc acaggtatcc agttcagtga aaacccacca acttttcacg
      961 agatcagaag cctggctgga cgattgtaca aagaaacatg tggagaagaa tttgctcagc
     1021 gtctacttgg ccacacatcg gagaagacaa caaaaatgta tcttgatgag agagaaaaaa
     1081 cgtacttact gctctgattt taacgtaaat ggattgttaa atgtattttg gttgtgatat
     1141 aaccaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg ggggagacat
     1201 ttgcaactaa ttgatttcaa atgcaattaa aaaaagaccg aatacgattc ctgtattcgg
     1261 tccagggaaa tggctcttgg gagagagccg tgcgctaaaa gttggcatta atgcaggctt
     1321 agttgccttg ccctttaaga atagatgacg acgccaggtt ttccagtttg cgtgcaaaat
     1381 ggtcaataaa aagcgcggtg gtcatcagct taaatgttaa aaaccgcccg ttctggtgaa
     1441 agaactgagg cggttttttt attggaaatc aaaaggctat tttaggtaat taacagagtt
     1501 tttcagctcg ttctataaac ggtgccagac tcattttttc gccgggattg ttaggatcat
     1561 caatctgaat caccgaaatg ggttgggcat tggtcttccc actggcaact tccttttgtg
     1621 cgatatcgtt taaaggatac tgcacgaggg tactcggatt aataacatac aaagcattac
     1681 ccggtcggca agtcagcatc acctcttcgc gattaaacgc ccatttgtct ttacccactt
     1741 caaaacgact gacggtaatc acctgcggtg cagccagcgc cgctgcagaa ctggtgagta
     1801 acagaaacgc cagaatactt tttttcatca ttttcgtaat ccatcaaaaa ggttcgagag
     1861 tcgcaagcag gctgatgatc accagcacta ccgcacctat cgcccattcg agtttagtca
     1921 tccacacaaa ccagggtgcc gcacgatctt cctcctgcct catgcgcggt acaagaacat
     1981 aacgattagc cagcgcaatg accaccataa tcatcaccag gatggctttg agcaataaga
     2041 gttggcccca gtaagtcgta agcgtggggg gaaatccggt aatcaacaat gcattaagca
     2101 cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca ccatgaaaaa cacatcagcg
     2161 cctgaatagc ctggtgtcgc caacggcctt tgatgagttg catacaccag agcaccggca
     2221 gtaatccccc aaaccacgcc gccgcacaaa tcaggtgaat cgcgtgatta gtctgatgga
     2281 ttttcgccgt taccccttca ttcagtgtcg catgccccat tcccgccagc agaataaatt
     2341 gcgcggtagt gagcataaac agcaaacgtg gcatattccg cggttgcata agggcgacga
     2401 tcaacgttac gagggcgaga acaatttgcc ataaccagac gccaccaaac tgcgtttgca
     2461 aaacagcctg ccaaatgttt ggcgaaaata catctgtcca tcccgttccc atcaacccgc
     2521 cctgaacggc gagcattgca gtggcactga tcaaactcca gacggcagcg tgctgttgta
     2581 aacgcagaaa acgcttcgcc agcaagcgac gaatcgtcaa gggggccagc caggcaccgt
     2641 acattgcgaa gccaaaaacc agcatcagcg aggtgaaatg gataaatcgc agcgcgatcc
     2701 aggtaaacgc cagcatgatt tatttcacgc taaaggtgta gtgtcctttc gttttatgcc
     2761 cgtccaccga aacaacatgc cagtcaacgg tgtaggttcc gggtttcagc gaatcggcca
     2821 gtggaacaat cagttgcttt tggtcctgct catttcgctt cgccggtaat gttttaatat
     2881 tttcgttttt tggccccgtg atttttgcac cactgaatcc tgtttccaca ccttccgaga
     2941 agtttaaagt gattgcctgc ggtgcagctg tcacttgcgc gtttgccgca ggatactgat
     3001 gcgttaaatg cgcatgtgcc cagacgaaag gtgtcaccaa cgaagtggtc aggattgcca
     3061 gcgcgtagcg aagggagcgt gcagttgaag ccatattatc tattcctttt tgtaataact
     3121 tttttacaga gcataacctt gtctaatgtc tgagtcgagg atcatcaatt ccggcttgcc
     3181 atcctggctc actcttagta acttttgccc gcgaatgatg aggagattaa gaatgctgaa
     3241 gaatctggct aaactggatc aaacagaaat ggataaagtg aatgtcgatt tggcggcggc
     3301 cggggtggca tttaaagaac gctacaatat gccggtgatc gctgaagcgg ttgaacgtga
     3361 acagcctgaa catttgcgca gctggtttcg cgagcggctt attgcccacc gtttggcttc
     3421 ggtcaatctg tcacgtttac cttacgagcc caaacttaaa taaaacttat acagggttac
     3481 actttcttac ataacgcctg ctaaattatg agtattttct aaaccgcacc cataatttgc
     3541 agtcattttg aaaaggaagt cattatgtcg ttctggaaag ttgcagcagc gcaatatgag
     3601 cctcgtaaaa cctcactcac cgaacaagta gctcatcatc tggagtttgt gagggccgcc
     3661 gccaggcaac aatgtcagct gttagttttt ccatcgcttt ctttactggg gtgtgattat
     3721 tcgcgacgtg cccttcctgc cccacccgat ctctcactgt tagacccgct ttgttatgcc
     3781 gcaacgacct ggcgaatgac catcattgcc ggccttcccg ttgaatataa cgatcgcttt
     3841 attcgtggaa ttgcggtgtt cgcaccgtgg cgaaaaacgc ctgggatcta ccatcagagt
     3901 catggtgcat gtctgggccg tcgttccaga accatcacgg tggtcgatga acaaccggaa
     3961 ggtatggata tggacccgac ctgttcactg tttacgacag ggcaatgtct gggagaaccc
     4021 gacctgctgg cgtcggcccg tcgcttacaa tttttttcac atcagtactc gattgctgtg
     4081 ctgatggcaa atgcccgtgg taacagtgca ctatgggatg aatatggtcg tctcatcgtt
     4141 cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt catcacaggg ttggcaaggc
     4201 gatatcattc cattacgcta ggctttttcg gcctggagta tgccatgttg cgcattatcg
     4261 atacagaaac ctgcggtttg cagggaggga tcgttgagat tgcctctgtt gatgtcattg
     4321 acggaaaaat cgtcaacccc atgagccacc tggtgcgccc cgatcgtcct attagtccac
     4381 aagcgatggc gattcatcgc atcaccgaag ccatggtcgc cgataaaccg tggattgaag
     4441 atgtgatccc acactattac ggcagtgaat ggtatgtcgc gcacaacgcc agctttgacc
     4501 gccgcgtact gcctgagatg cccggtgagt ggatttgcac catgaaactg gcccgtcgct
     4561 tgtggccagg aatcaagtac agcaatatgg cgttatataa aacacggaag ctcaatgtac
     4621 agacgccgcc gggcctgcat catcaccgcg cgttgtatga ctgttatatc accgccgcgt
     4681 tgcttatcga tattatgaac acctccggct ggacggcaga acagatggcc gatatcaccg
     4741 gacgtccgtc gttgatgacg accttcacct ttggcaaata ccgtggcaaa gcggtttccg
     4801 acgttgccga acgcgatccg ggctatctgc gctggttatt taataacctg gacagcatga
     4861 gcccggagct gcgtttaaca ctgaaacatt atctggaaaa tacttaatcc gcaggcgtag
     4921 cgggtaatgt tccctgcgcc agcgcgacca gaaaagcata ttccatcgct acgccttcgt
     4981 acgatttaaa gcggccagat ttaccgccat ggcctgagtc catgtcggta cagagcaata
     5041 aaagatggtt atcggttttc agctcgcgca atttagcgac ccattttgcc ggttcccaat
     5101 attgcacctg agaatcgtgt aaaccggtcg ttaccagtaa atgcggataa gcctgtgcgg
     5161 tgacgttgtc atacgggctg tagcttttca tgtactcgta atattgcgga tcctgcgggt
     5221 taccccactc ttcaaactca ccagtggtaa gaggaattga ttcatcaagc atcgttgtta
     5281 caacatcaac aaacggtacc tgggcgataa cgccgtggaa taattccggg cgttgattaa
     5341 ttgcaacgcc catcaacatg ccccccgcac tcccgcccat cgcataacaa agcgaaggag
     5401 agccatagcc cagttttaac aatgcatcgc aggcatcaag ataatcatta aacgtatttt
     5461 tcttcttcag aaattttccg tcttcgtacc attgttgccc cagctcacca ccgccgcgaa
     5521 catggacaat ggcgtagaca aagccacgat ctaacaaact caagcggcta aaactgaaat
     5581 cggcatcaat acttgcgccg taagaaccat agccatacac cagcaacggg ttgtgtcctt
     5641 tgcgaaaatg tttgcgatgg tagaccaacg aaaccggaac ttcgacgcca tcacgggcga
     5701 ctatccacag gtgttcactg cggtaattcg ccgcatcaaa accaggaact tccgtttgtt
     5761 ttaatacacg acgctcatcg gtatccatat ccagttcaaa caaagtgtct ggtgtggtca
     5821 tggaagaata accataacgc aatcgcgcgg tttcaggttc tggattgtag gcaatccagg
     5881 tcacataggc tggatcatca aaggcaatac caatgacttc ccgggtcttg cggttaattt
     5941 ggcgcaaact ggttaacccg cgctgacgct cttcaaccac cagccagtcg gtaaacagcg
     6001 taaacccttc cagcatgatg ttttcgcatg gcggaattaa ctcttcccac tgttgctcat
     6061 cacgcatacg ggtacggtat aagccaaagt ttttgccgtg gcggttggaa cgcagataaa
     6121 aacgatgctg gtagtgatca aggctgtatt cgtgatcttt gcggcgcggc agaaaaacaa
     6181 acggctcggc atcggccatt tccgcgtcca gcaggcgaac ttcactggtg gtggcgctgg
     6241 ccaaatgaat gactacatag tgcttcgagg tcattttatg caggctgacg taataggtat
     6301 cgtctttttc ttcgtagatc agtttatctt gcgatgctgg cgtaccgatg gcgtgacgcc
     6361 agacctgata aggcagcagc gtcaccggat gcttgcgaac atagtagaaa gtccaggagt
     6421 catttgccca gacaaagctg ggttcaacgt tatccagcag ttccgggtac cagttaccag
     6481 tttccagatt acgaaaacga atgccgtact ggcgtcggga aagaaaatct tctgccagcg
     6541 ccataatggt gttatcgggc gtaatcgcca ttccgcccat cgaataaaac tcactatgag
     6601 ctgcgcgctt attggcatcg agcaatattt cccactcatc ccactcttca ctgaatgccg
     6661 attgacgctg gtagatagca tattcacagc ctggttcata aatatgctga tagcggtagc
     6721 catttttgat gtagggcgca gaaacttctc gttgcggaat gcggtcgatg atttccttta
     6781 agatgcgatc ctgcaaggct tgttgtgagg ccatcacccg atgaccgtaa ctattttctt
     6841 gttgcaggta gtccaggact tctggctgag aacgcgtatc atcccgcagc cagtagtaat
     6901 tatcgatgcg cgtatcgcca tgaagcgtca tggcgtgggg aatgcgggcg gcttttggta
     6961 gcatgttatt gttctttctg gttgaaacat cttataaggg tggcaaaact caccggggat
     7021 gcaagcgaaa caggggagtc attgcttaga tgatgacagg taatggcacg gatatcgaat
     7081 gttatgcaga cagagaaact cagcctgttc aaatggctgt gcggttctgg atagcccgaa
     7141 atagtcaact tcaggctatc cagagagcgg aattattccg ccaaagtgcg tttttgctgt
     7201 tcgagatcgc gttcaatgcc gtcacgaaca tcctggggga ttttcagcgc gtcacccagt
     7261 gcattcaggt aactgcgttc cataaaatgg tcaatatcaa tagccgcgca actcaggaaa
     7321 tagatttcca gcgcctcttc ttcattgcgg accccggtag ccaggcgttg tggatccagc
     7381 ggttgttcga ttgcctgctc aatgagtaca cgcccctgct cttccacgcc ggcttcacgc
     7441 aattgctggt cgattgccgc acgttctttg gcatcaatat gaccatcact tttagcggca
     7501 aagaccagcg caaggatcaa acgttctgta cgctcatcca gcggcgtact ttgcgcgcca
     7561 aactgcggtt cgtcctgatg cgccgcgcga attttatctt tgtatttatt ccacagcacc
     7621 gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg tgccatattt cgtaagaagt
     7681 ttacgtgctg atttatttgc gaccagcagc ccagccagtc cgcctaatgc gcctggcact
     7741 aacagtttgc ccaatccctg atccgcagac gaggaggtag aagaactgct ttgcccaagc
     7801 agggattgca gttgatttaa ccagttagcc atatttgctc ctcaataacc atttttagtg
     7861 cctgtagcat agcggatgag gatgtcagaa aatgtatatc gagacaaaag atgcgcaaat
     7921 aagcggggcg gtaggccgtt attcaaagaa attcgcgcca ggtaaggtat ttactgtata
     7981 tcaggtgata agggtatttt aggtgaaagt agtttgtcgt tttgagtggc attgtttgat
     8041 gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc tacgattgtg gtgcgttctg
     8101 gggcggacaa ggcactcgcg ccgcatccac caacggtcaa tcgaattatc agaagccatt
     8161 aacgccgctg atattccgcc attcccgctt tgcagtccac gctgacctgg taatgaatat
     8221 cggcactttt accacgcacg gttagcggta ctgaccattt atcatcttta ccctgaatgt
     8281 cctgcaaact gacccaagcc acgggatcgg cctgaccgac aattttttga tcatctgccc
     8341 aacgcgccac gcgattttgt tgataatcac gttttacgct cgcggcaatt ccggcagcat
     8401 ccagaccttc acactttggg aaagtgaccg acttgctggt ttcattattg gcagcgaaaa
     8461 ctgatgcgca ggcagaaacc aacaacagcc ctaaaaacgc ccctcttttt ttcatgtttt
     8521 tctccatagc acaatgattc aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc
     8581 ttcaggccgc gtgtgaggca tcttcctctt cggattcagc accgacgacc tgtggggacg
     8641 gtaatttgcc ggttttcaga atggtgctca gcacatcttt ttgttctgcc agccatagcg
     8701 aaagggcttc acgttgctcg tcttccattt gcactggaga atttgtcagc caggtgtcaa
     8761 gcagatccgc cgtatcaagc attttgtcat aagcatcggc ttcctttttg ctggtaaacg
     8821 acattttctc ttcgccctca cgaatgacta cgtatttaac ttcaaccgcc atttgcagcc
     8881 tctcataata actgtgattt tatacagtat atttcttttc ggttgagaaa tcaacatcag
     8941 caataaagac acacgcaaac gttttcgttt atactgcgcg cggaattaat caggggatat
     9001 tcgttatgac gttattaggc actgcgctgc gtccggcagc aactcgcgtg atgttattag
     9061 gctccggtga actgggtaaa gaagtggcaa tcgagtgtca gcgtctcggc gtagaggtga
     9121 ttgccgtcga tcgctatgcc gacgcaccag ccatgcatgt cgcgcatcgc tcccatgtca
     9181 ttaatatgct tgatggtgat gcattacgcc gtgtggttga actggaaaaa ccacattata
     9241 tcgtgccgga gatcgaagct attgccaccg atatgctgat ccaacttgaa gaggaaggac
     9301 tgaatgttgt cccctgcgct cgcgcaacga aattaacgat gaatcgcgag ggtatccgtc
     9361 gcctggcggc agaagagctg cagctgccca cttccactta tcgttttgcc gatagcgaaa
     9421 gccttttccg cgaggcggtt gctgacattg gctatccctg cattgtaaaa ccggtgatga
     9481 gctcttccgg caaggggcag acgtttattc gttctgcaga gcaacttgct caggcatgga
     9541 agtacgctca gcaaggcggt cgcgccggag cgggccgcgt aattgttgaa ggcgtcgtta
     9601 agtttgactt cgaaattacc ttgctaaccg tcagcgcggt ggatggcgtc catttctgtg
     9661 caccggtagg tcatcgccag gaagatggcg actaccgtga atcctggcaa ccacagcaaa
     9721 tgagcccgct tgcccttgaa cgtgcgcagg agattgcccg taaagtggtg ctggcactgg
     9781 gcggttatgg tctgtttggt gtcgagctgt ttgtctgtgg tgatgaggtg attttcagtg
     9841 aggtctcccc tcgtccacat gataccggga tggtgacgtt aatttctcaa gatctttcag
     9901 agtttgccct gcatgtacgt gccttcctcg gacttccggt tggcgggatc cgtcagtatg
     9961 gtcctgcagc ttctgccgtt attctgccac aactgaccag tcagaatgtc acgtttgata
    10021 atgtgcagaa tgccgtaggc gcagatttgc agattcgttt atttggtaag ccggaaattg
    10081 atggcagccg tcgtctgggt gttgcgctgg cgaccgctga gagtgttgtt gacgccattg
    10141 aacgcgcgaa gcacgccgcc ggacaggtaa aagtacaggg ttaaacccgg caaaaaaacg
    10201 ctacaaaaat gcccgatcgt agatcgggcg ttttgacttt tacagcttag cgccttctac
    10261 agcttcacgc gccagcttag taatgcggtc gtaatcgccc gcttccagcg catctgccgg
    10321 aaccagccag gaaccaccga tgcacagcac gcttttcagc gccaggtagt cacggtagtt
    10381 agccggagaa ataccacccg tcgggcagaa acggacctgg gagaacggac ccgcgatcgc
    10441 ctgcagggct ttcacgccgc cgttagcttc agccgggaag aatttgaact ctttcaaacc
    10501 gtagtccata cccagcatca gttcggaaac agtgctgatc cccggaatta gaggaatagt
    10561 cccttcggta gcagctttca gcagcggctc ggtcagaccc gggctaattg cgaactgtgc
    10621 acccgcttca gtgacttctg ccagctgctg tggattcagc accgtaccgg cacccacaat
    10681 cgcttcaggc acttctttgg cgatagcacg gatagcgtca actgcacact cggtacgcag
    10741 agtcacttcc agaacgcgca ccccaccagc aaccaacgct tttgccatcg gcactgcgtg
    10801 ttccagtttt tttaccacga taaccggtac aaccgggccg gtggtcagga ttgattctgc
    10861 acttgttttc cagtttttca tcagagtttt ctctcgcctg attacaaatt tgtcgtctta
    10921 aaaagtgata caggttgcgc cctgttcggc accggacagt ttttcacgca aggcgctgaa
    10981 taattcacgt cctgtcccca cgcgtgacgc gctcaggtca ggaatgtgcg gttcgcgagc
    11041 agccagttcc gcttcgtcta ccagcagcgt cagttcgcct gtctgtccat tcacacgaat
    11101 gatgtccccg tcgcgcactt ttgccagcag cccgccatcg taggcttctg gtgttacgtg
    11161 gatagctgac ggcactttac ctgaagcgcc ggagagtcgt ccatcggtaa ctaacgcaat
    11221 tttgaaacac cggtccaata atacaccaag tggcggcatg agtttatgta attctggcat
    11281 tccgttcgct tttggcccct gatgacggac aacaacgaca caatcgcggt ccagcaaacc
    11341 cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa acaaccgctg gcgcttcaat
    11401 cacctggttc tcaaccggca cggcagaggt tttcataacc gcacggccca ggttaccgct
    11461 taacactttt gtcccaccat gatgagagaa aggttgttcg aaggaagcga tcacattgct
    11521 gtcgagtgat ttttccgccc cttcccgcca gtcctgttca ccattattca gccatggttc
    11581 aagggtataa cgagacagac caaagcctgc caccgtattg acatcttcat gcagcaggcc
    11641 tgctttgagc agttcacgca ccagaaccgg tacgccacct gccgcctgga agtggttaat
    11701 atcggccgga ccgttcgggt agagacgtgc catcagcggt acaacatcag aaaggtcaga
    11761 gaagtcatcc cagttaatct gaataccggc cgcgcgcgcc atcgccacca ggtgcatggt
    11821 gtggttagtg gaaccaccgg tcgccagcag tgcaacgata ccgttcacca ccactttctc
    11881 atcgatcatc ttaccgatcg gcatccattc attaccatta ccggtcatgc gtgtaacctg
    11941 acgcgcagcg gcagcggtca aagcatcgcg cagcggagaa tccggatgaa caaaagaaga
    12001 gcctggcaac tgcatcccca taaattccac caccatctgg ttggtgttgg cagtaccgta
    12061 gaaagtacat gttcccggcg catggtaaga cgcggcttct gactccagta aggccatgcg
    12121 gtccacttta ccttcggcat aaagctggcg aatacgcact ttttctttat ttggcaaacc
    12181 gcttgccatc ggtccagacg gcacaaacac cgcaggcaaa tgaccaagcg acagggctgc
    12241 catcgtcaga cccgggacaa tcttgtcgca cacaccgagg aacagagcac catcaaacat
    12301 gttatgggac agccccaccg ccgcagacat cgctatcact tcgcggctta gcagcgacaa
    12361 ttccattcca tcctgcccct gggtgacacc atcacacatc gccggaacac cgcccgcaac
    12421 ctgaccaacc gcattcgctt catgcagggc tttacgaatg atttctggat agtgttcata
    12481 aggctggtgc gcggagagca tgtcgttata ggaggtgatg atggcgatat tgttacgcaa
    12541 catgcttttc aaagaggctt tgtcttctgg ctggcaggca gcgaaaccgt gtgccaggtt
    12601 accgcatgcc aactgcgaac gatgaacggt cgacgttttc gcttgttcta tccgggcgag
    12661 ataagcagag cgagtctcgc gcgaacgttc aatgattcga tttgttacgc ataacaattg
    12721 tggattcata aaggctcctg aaattgagtt gtcagagcag gatgattcac aacgcgtttc
    12781 attcagagga tttatgagtg aaacgcctgt aaccggagct catagggcaa aaacgtttca
    12841 gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg aggcctgaaa gtgtaaaaat
    12901 tgttctacaa tctgtgcaag atcatgttac cggtaaaata accataaagg ataagcgcag
    12961 atattactca aactcattcc aggaacgacc atcacgggta atcatcgcca ccgaggcaac
    13021 gggtccccag gttccggcct gatacggttt cggcgcatca ttgtccatcg cccacgcctc
    13081 agtaatggag tctacccatt tccaggcttc ttccacttca tcacgacgta caaacagtgc
    13141 ctgaatacca cgcatggttt ccagcagcag acgttcatag gcatccgcca gatgcgtctg
    13201 attaaaggtt tctgaatagc tcagatccag cttggtgatt tgcaggttat gtttgtggtc
    13261 aaggccagga actttattca gtacctggat atccacgcct tcatcaggtt gcagacggat
    13321 agtcagttta ttctgcggca gatcctgcca cgactcttta aacagattca gttcaggtgt
    13381 tttgaaatag accacgactt cagaacattt ggtcggcaga cgtttaccag tacgcaggta
    13441 gaatggcaca ccggcccagc gccagttatc aatgtcgacg cggatcgcca cgaaggtttc
    13501 tgtattgctg ctcttgttcg cgccctcttc ttccagatat cccggcactt ttttgccctg
    13561 ggcgaagccc gcagtatatt gcccgcgtac ggttttttcg cgtacgttgg agcggtcgat
    13621 gcggcgcagg gacttcagta ctttcacttt ttcatcgcgg atgctgtctg cgctcaggtc
    13681 agatggcgga gacatcgcaa tcatgcagag aatttgtagc aggtggttct gaatcatgtc
    13741 acgcatctga ccggctttat caaaatagcc ccagcgccct tcgatcccca cttcttctgc
    13801 cacggtaatc tcaacatgat caatggtgcg attgtcccag ttattcacaa acagggagtt
    13861 agcaaaacgc agcgccaaca ggttcagcac cgtttcttta ccaagatagt ggtcgatacg
    13921 gtaaacctgg cactcctcga agtattcgcc aacctgatca ttgatttcct gcgaggtcgc
    13981 cagcgacgtc cccagcggtt tctccatgac tacgcgtgcc ggtttagcat tcagttttgc
    14041 ctcgccaagc cctttgcaaa ttgcgccaaa agtgctgggc ggcatggcaa agtagttaat
    14101 ggtgatacga tttttttgat ccagcatcgc gccgagacgg ttgaatgcag cagtgtcatt
    14161 gacatcgaga ttacaaaaat ccagacgtgc actcagggtg tcccataaac cttcatcaat
    14221 ggtttctttc atgaaagttt cgagcgcctc gcggacaact ttggtatatg ccgctttatc
    14281 ccagtcagca cgccctacgc cgataatccg ggtgtccggg ttgagctgac cggctttttc
    14341 cagttgatac agggaaggca gcaatttacg acgcgcaagg tcgcctttcg cgccgaaaat
    14401 gaccaggtca caggcctggg ttgtttgcgt taccgccatg tcattctcct taagttaact
    14461 aacccggtac ttaagccagg gtatacttgt aattttctta cggtgcactg tactgctttt
    14521 acgagcttgc gaaaactgta aacgcttatc cacccgtgcg attacgggaa aagcgcgcaa
    14581 agtgcggcaa aactgataaa aaaatcatcg ttttccattc ggtaaaaatc tgacactgat
    14641 catgttatga aaaaaaataa caactttttt atctgctttt gtcattaaca cggcacacag
    14701 gcgtaatatc tgacaaaact gcatttcgat ttctttcagt gcggaaatcg tcattacccg
    14761 tgagtctctt tacatcatga atatgctgga aaaaatccag tctcagctgg aacatttgag
    14821 caaatcagag cgcaaagttg ccgaggtcat tctggcttcg cccgataacg cgatccattc
    14881 gagtattgct gctatggcac tggaagccaa tgttagcgaa ccgacggtga atcgtttctg
    14941 tcgcagcatg gacacgcgcg gttttcctga ttttaaactt catctggcac agagtctggc
    15001 gaatggcact ccctatgtta atcgcaatgt caatgaagat gacagcgttg aatcatacac
    15061 agggaaaata tttgagtccg caatggcaac gcttgatcat gtccgtcatt cactggataa
    15121 atctgccatc aaccgcgccg tcgacttgct cactcaggca aaaaaaatcg cctttttcgg
    15181 attaggctct tcagccgccg ttgcccacga tgcgatgaat aagttctttc gttttaatgt
    15241 tccggtggtg tactccgatg atatcgtgct gcaacgcatg agttgtatga attgtagcga
    15301 cggagacgtg gtggtgctga tttctcacac tggaagaaca aaaaatctgg tcgagctggc
    15361 gcagctggct cgcgaaaacg acgcaatggt gattgccctc acctctgcgg gtaccccgct
    15421 cgcccgggaa gcaacgctgg caattaccct cgacgtaccg gaagatactg acatttatat
    15481 gcccatggtt tctcgacttg cacagctgac cgtgatagat gtgctggcga caggatttac
    15541 tttgcgacgc ggtgcaaaat tcagagataa cttgaagcgg gtcaaggaag cgctgaagga
    15601 atcgcgtttt gataagcagt tacttaattt aagtgacgat cgctaaaaac gactgtcact
    15661 gtcctaatct tatacgacat ccgaatgaga ttaatttatc gccatcgcgg cgttatttca
    15721 ttcggatttc atgttcaagc aacacctggt tgtttcagtc aacggagtat tacatgtcca
    15781 gaaggcttcg cagaacaaaa atcgttacca cgttaggccc agcaacagat cgcgataata
    15841 atcttgaaaa agttatcgcg gcgggtgcca acgttgtacg tatgaacttt tctcacggct
    15901 cgcctgaaga tcacaaaatg cgcgcggata aagttcgtga gattgccgca aaactggggc
    15961 gtcatgtggc tattctgggt gacctccagg ggcccaaaat ccgtgtatcc acctttaaag
    16021 aaggcaaagt tttcctcaat attggggata aattcctgct cgacgccaac ctgggtaaag
    16081 gtgaaggcga caaagaaaaa gtcggtatcg actacaaagg cctgcctgct gacgtcgtgc
    16141 ctggtgacat cctgctgctg gacgatggtc gcgtccagtt aaaagtactg gaagttcagg
    16201 gcatgaaagt gttcaccgaa gtcaccgtcg gtggtcccct ctccaacaat aaaggtatca
    16261 acaaacttgg cggcggtttg tcggctgaag cgctgaccga aaaagacaaa gcagacatta
    16321 agactgcggc gttgattggc gtagattacc tggctgtctc cttcccacgc tgtggcgaag
    16381 atctgaacta tgcccgtcgc ctggcacgcg atgcaggatg tgatgcgaaa attgttgcca
    16441 aggttgaacg tgcggaagcc gtttgcagcc aggatgcaat ggatgacatc atcctcgcct
    16501 ctgacgtggt aatggttgca cgtggcgacc tcggtgtgga aattggcgac ccggaactgg
    16561 tcggcattca gaaagcgttg atccgtcgtg cgcgtcagct aaaccgagcg gtaatcacgg
    16621 cgacccagat gatggagtca atgattacta acccgatgcc gacgcgtgca gaagtcatgg
    16681 acgtagcaaa cgccgttctg gatggtactg acgctgtgat gctgtctgca gaaactgccg
    16741 cagggcagta tccgtcagaa actgttgcag ccatggcgcg cgtttgcctg ggtgcggaaa
    16801 aaatcccgag catcaacgtt tctaaacacc gtctggacgt tcagttcgac aatgtggaag
    16861 aagctattgc catgtcagca atgtacgcag ctaaccacct gaaaggcgtt acggcgatta
    16921 ttaccatgac cgaatcgggt cgtaccgcgc tgatgacctc ccgtatcagc tctggtctac
    16981 caattttcgc catgtcgcgc catgaacgta cgctgaacct gactgctctc tatcgtggcg
    17041 ttacgccggt gcactttgat agcgctaatg acggcgtagc agctgccagc gaagcggtta
    17101 atctgctgcg cgataaaggt tacttgatgt ctggtgacct ggtgattgtc acccagggcg
    17161 acgtgatgag taccgtgggt tctactaata ccacgcgtat tttaacggta gagtaatctc
    17221 acctgccccg gccgctgatg gccggggtat ttagcaacac ataaattagg actacttatt
    17281 caatccaatc ataatcacag ccccctgtaa aatgaataca gggatagcct gaatggagat
    17341 ctaaatagct tgcaagacgt cgcatacccg cttcctctgc aacactatgt ccaataactg
    17401 ccatcggtaa gccactatct ttggagtatt gtgtctgcac ccatgtttta attgaatcgt
    17461 taatcgtaat ataataatca gcctgatatt ccatatattg tattggatca caaaaacagc
    17521 ctgccccaat gccaacagtt ctgactactc tggctttatc tccataaaat tgaatgccag
    17581 gaagattata tattttcaaa tttttggcaa aacgtttggt aatgttaatg gcactgtcag
    17641 ggtctatagc atatacacag tacatctcat cagaagcaac aatatttgta acatccagtt
    17701 ctaactgctg agccaaagcc ttagacattc cgaacgtagg ctctcgatcc atgacatcat
    17761 ggcaacgaat gattaccaaa ttatttttat gcaaaaactc ctttttttgc tcaattattt
    17821 ttaaataact gagttcaccg cgagtataat ccattatttt tctggcccat gaaaactcaa
    17881 gatcgtcacc ctccagatca gcataagaat aaaatgtagg ctcatgtgta ataatgatat
    17941 tgacaccttc aaaaacagcc tttcttaaaa catccattga ggcaatccag caggtcccaa
    18001 ttttattaat aagtgtcgcc tcagcaccaa aaataatttt atcaacagtt ggttgcgaaa
    18061 cagcaaccat tgaatttaaa aaacaattaa gatccgcggc tgttttaata tgatgcattc
    18121 aacatccttc acaggtttca tcagcaataa aataatttca acatttctgg gaaacgtttt
    18181 gcccatgcag actcagagtg ggtatcacct ccccataaa
//
LOCUS       DAZPJP010000069        17956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPJP010000069 DAZPJP010000000
VERSION     DAZPJP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.069"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHU9J_002174"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHU9J_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1905704.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHU9J_002175"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHU9J_002175"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1905705.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="ACHU9J_002176"
     CDS             4388..4639
                     /locus_tag="ACHU9J_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1905706.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHU9J_002177"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHU9J_002177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1905707.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="ACHU9J_002178"
     CDS             5944..6702
                     /locus_tag="ACHU9J_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1905708.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="ACHU9J_002179"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="ACHU9J_002179"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1905709.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHU9J_002180"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHU9J_002180"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1905710.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="ACHU9J_002181"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="ACHU9J_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="ACHU9J_002182"
     CDS             complement(9556..10176)
                     /locus_tag="ACHU9J_002182"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1905711.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHU9J_002183"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHU9J_002183"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1905712.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="ACHU9J_002184"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="ACHU9J_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1905713.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="ACHU9J_002185"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="ACHU9J_002185"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1905714.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="ACHU9J_002186"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="ACHU9J_002186"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1905715.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHU9J_002187"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHU9J_002187"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1905716.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="ACHU9J_002188"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="ACHU9J_002188"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1905717.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="ACHU9J_002189"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="ACHU9J_002189"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1905718.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAZPJP010000070        17809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPJP010000070 DAZPJP010000000
VERSION     DAZPJP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.070"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            94..772
                     /locus_tag="ACHU9J_002190"
                     /pseudo
     CDS             94..772
                     /locus_tag="ACHU9J_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            816..5666
                     /locus_tag="ACHU9J_002191"
     CDS             816..5666
                     /locus_tag="ACHU9J_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR1905719.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6034..7689
                     /gene="lldP"
                     /locus_tag="ACHU9J_002192"
     CDS             6034..7689
                     /gene="lldP"
                     /locus_tag="ACHU9J_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1905720.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7689..8465
                     /gene="lldR"
                     /locus_tag="ACHU9J_002193"
     CDS             7689..8465
                     /gene="lldR"
                     /locus_tag="ACHU9J_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1905721.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8462..9652
                     /gene="lldD"
                     /locus_tag="ACHU9J_002194"
     CDS             8462..9652
                     /gene="lldD"
                     /locus_tag="ACHU9J_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1905722.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9836..10309
                     /gene="trmL"
                     /locus_tag="ACHU9J_002195"
     CDS             9836..10309
                     /gene="trmL"
                     /locus_tag="ACHU9J_002195"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1905723.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10362..11183)
                     /gene="cysE"
                     /locus_tag="ACHU9J_002196"
     CDS             complement(10362..11183)
                     /gene="cysE"
                     /locus_tag="ACHU9J_002196"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1905724.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11263..12282)
                     /gene="gpsA"
                     /locus_tag="ACHU9J_002197"
     CDS             complement(11263..12282)
                     /gene="gpsA"
                     /locus_tag="ACHU9J_002197"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1905725.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12282..12749)
                     /gene="secB"
                     /locus_tag="ACHU9J_002198"
     CDS             complement(12282..12749)
                     /gene="secB"
                     /locus_tag="ACHU9J_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1905726.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12812..13063)
                     /gene="grxC"
                     /locus_tag="ACHU9J_002199"
     CDS             complement(12812..13063)
                     /gene="grxC"
                     /locus_tag="ACHU9J_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1905727.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13205..13636)
                     /locus_tag="ACHU9J_002200"
     CDS             complement(13205..13636)
                     /locus_tag="ACHU9J_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1905728.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13881..15425
                     /gene="gpmM"
                     /locus_tag="ACHU9J_002201"
                     /gene_synonym="pgmI"
     CDS             13881..15425
                     /gene="gpmM"
                     /locus_tag="ACHU9J_002201"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1905729.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15459..16718
                     /gene="envC"
                     /locus_tag="ACHU9J_002202"
     CDS             15459..16718
                     /gene="envC"
                     /locus_tag="ACHU9J_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1905730.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16722..17681
                     /locus_tag="ACHU9J_002203"
     CDS             16722..17681
                     /locus_tag="ACHU9J_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1905731.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17668..>17809)
                     /locus_tag="ACHU9J_002204"
     CDS             complement(17668..>17809)
                     /locus_tag="ACHU9J_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1905732.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4458 a         4613 c         4668 g         4070 t
ORIGIN      
        1 ctgtcattaa tgtaaagcaa catagatcac caataataca atggacaact ctaccggcat
       61 tgatcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt
      121 gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag
      181 ccagaaagta gtttattgaa acaacaagaa gaacattaaa attgatctac ttgaaaaaca
      241 acaagcacag ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag
      301 cagtacaaag actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat
      361 ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc
      421 tttagcgggt cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc
      481 actggtcaac ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgg
      541 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      601 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
      661 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
      721 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt
      781 agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct
      841 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
      901 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
      961 ttggggcatt ggcgaatgcc gggaatgaca acggccaggg tgttgattac ggtagtggat
     1021 cagctggcga cagctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
     1081 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
     1141 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
     1201 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
     1261 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
     1321 cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa gctactgaga
     1381 ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca atggcgctgg
     1441 gagcaagtag cgtcgcatct gaagaaaacg caattgccct ggggcgtagc agtgtagcta
     1501 gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg
     1561 cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg
     1621 caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta
     1681 tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata ggccagggca
     1741 acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa
     1801 acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta
     1861 gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt
     1921 tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa
     1981 atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta
     2041 gcgactcggt agcaaaaacg cttggaggag gggctgcagt agatgttgat gacggtactg
     2101 ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg
     2161 ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac agcgctgctc
     2221 atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc acgatttcag
     2281 cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac gatgtcgaag
     2341 ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaataca gcaagtattg
     2401 ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg
     2461 ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca
     2521 gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg
     2581 gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata
     2641 acacttccaa tattgccact aacaccacca acatctctaa tctgactgag acggtgacta
     2701 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg
     2761 gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg actggcagca
     2821 ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca
     2881 ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2941 gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca
     3001 ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg
     3061 ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca
     3121 atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa
     3181 attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg
     3241 gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg
     3301 gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg
     3361 cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg
     3421 gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg
     3481 attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac gacgctctgc
     3541 tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa
     3601 ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga
     3661 ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg
     3721 cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt
     3781 acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg tgggatgaga
     3841 ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca
     3901 ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt
     3961 tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta
     4021 acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt cgtactaacg
     4081 acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca gctgtaggtt
     4141 ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc agcagcaccg
     4201 ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt gccaaaggtt
     4261 ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc actgacggcg
     4321 aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc atcaacgtag
     4381 ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac gctattggag
     4441 cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa gactcactgg
     4501 ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt aatgcaggta
     4561 ttggtatcgg tttgaacact ctggttctag ctgatgcgat caacggcatt gctatcggca
     4621 gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct cagaccaccc
     4681 gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac tctgtaggtg
     4741 agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca gctggttcag
     4801 cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc gtagcgcaaa
     4861 atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta
     4921 atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac ttcaagacca
     4981 acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt
     5041 ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa
     5101 atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccggtg
     5161 ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggcgtgc
     5221 gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca
     5281 acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga
     5341 actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat cagcggatgg
     5401 ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg
     5461 caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg
     5521 gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca
     5581 atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg
     5641 cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg
     5701 ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgccccggg tcaacaatct
     5761 aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc cccacaagaa
     5821 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5881 acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta tccctacaca
     5941 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
     6001 tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca aaactacgat
     6061 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
     6121 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtagcaatc
     6181 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     6241 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
     6301 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6361 ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6421 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggcctg
     6481 ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca
     6541 tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat cgacagcttt
     6601 gagattggcc agatggtggg gcggcagcta ccgtttatga ccattatcgt gctgttctgg
     6661 atcatggcga ttatggacgg ctggcgcggt atcaaggaga cgtggcctgc ggtcgtggtt
     6721 gcgggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
     6781 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
     6841 cagccagtgc gcgtattccg ttttggtgat ttgggggcgt caaagattga tatgacgctg
     6901 gcccacaccg gttacactgc gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg
     6961 acagctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt
     7021 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt
     7081 atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
     7141 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
     7201 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctacccatc
     7261 tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg actgtcatca
     7321 acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7381 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
     7441 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
     7501 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
     7561 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7621 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
     7681 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7741 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg
     7801 cgatgcaact cggcgtatca cgtaattcac tgcgcgaggc gctggcaaaa ctggtgagcg
     7861 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
     7921 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
     7981 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
     8041 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
     8101 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     8161 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
     8221 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
     8281 aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa
     8341 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8401 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
     8461 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8521 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
     8581 agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg acttaagcct
     8641 ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac cggtgggttt
     8701 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
     8761 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
     8821 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
     8881 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
     8941 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg
     9001 ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     9061 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
     9121 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     9181 ccgcgatttc tgggatggcc cgatggtgat taaagggatc ctcgatccgg aagatgcgcg
     9241 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9301 ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga
     9361 tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
     9421 gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg
     9481 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9541 gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc aggggctggg
     9601 taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc
     9661 gccccctcac cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     9721 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     9781 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     9841 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
     9901 cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa
     9961 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
    10021 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
    10081 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
    10141 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
    10201 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
    10261 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10321 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
    10381 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10441 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
    10501 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
    10561 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10621 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10681 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
    10741 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10801 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    10861 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    10921 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    10981 tccaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    11041 atgtagctca gtgcactacc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    11101 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    11161 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    11221 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
    11281 gtccttgcgt gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11341 taatacttga taaatttcct cggttattgg catttctacg ccgaagcgat gcgccagttc
    11401 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11461 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
    11521 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11581 acccagcgcc gcgccaagac gcgacatttc agccagccca cgggtgatca gtgccgtacg
    11641 cgcattcgca ccaaaaccga taccgtcgga catccccgcg ccaatggcaa tgacgttttt
    11701 caccgcaccg ccaagctgca caccgatgaa atccgggttg ctgtaaacgc ggaaactttt
    11761 gccacagtgc agcaattgtt ggagatcatc ggcaaaggtc tggtcggtag aggccaacga
    11821 aatagccgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    11881 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    11941 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    12001 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc
    12061 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    12121 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    12181 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12241 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12301 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaatc
    12361 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca cgctggtgat gcactcacga
    12421 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12481 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12541 tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12601 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12661 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12721 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12781 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
    12841 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    12901 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    12961 cgatcggcaa ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    13021 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    13081 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    13141 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    13201 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13261 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13321 gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa
    13381 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
    13441 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13501 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13561 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13621 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13681 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13741 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13801 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    13861 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    13921 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    13981 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    14041 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    14101 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    14161 ttgctaatcc agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac
    14221 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14281 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14341 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14401 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14461 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14521 ttcaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14581 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14641 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14701 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14761 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    14821 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    14881 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    14941 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    15001 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    15061 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    15121 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    15181 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15241 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    15301 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15361 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15421 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15481 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15541 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15601 ccacaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15661 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15721 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15781 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    15841 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    15901 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    15961 aaacgcgtga agaagtcgcc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    16021 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    16081 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    16141 aactgcgtgc caacgaatcc cgtctgcgta acagtattgc ccgtgcggaa gccgcggcga
    16201 aagcgcgtgc tgaacgagaa gcgcgtgagg ctcaggcagt tcgcgacagg cagaaagaag
    16261 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16321 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc agttcgtggc cctacgctgc
    16381 atcgctatgg cgagcagcta cagggtgaat tacgctggaa aggaatggtt atcggtgcct
    16441 ctgaaggtac tgaagttaaa gcaattgccg acggtcgggt gattctggct gactggctgc
    16501 aaggctacgg tctggtggtg gtggttgagc acggtaaagg cgatatgagt ctttacggct
    16561 ataatcaaag cgcgctggtg agcgttggtt cgcaggttcg tgcgggccag ccaattgcac
    16621 tggtgggcag cagtggcggt cagggcagac cttcactcta tttcgaaatt cgccgccagg
    16681 gtcaggccgt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16741 acgttcttgc atttgccgct ctgttggcgc tctcctcccc tgtaattgct ggcaaacttg
    16801 ccatcgtcat tgatgatttt gggtatcgcc cgaacaacga aaaccaggtg ctggcgatgc
    16861 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    16921 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    16981 agccgctgga gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta
    17041 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caataaccac atgggcagca
    17101 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttataatc
    17161 tttacttcct cgacagcgta accattggta atacccaggc gatgcgcgcc gcgcaaggca
    17221 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcacagaac gaagcggaca
    17281 tccgtgtgca attcaatcgc gcaattgacc tagcgcgtcg caacggatca accattgcca
    17341 ttgggcatcc tcacccttca acggtacgcg tgttgcaaca gatggtttat aacctgccgc
    17401 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacgtcta
    17461 caccgccgaa aaacactgtg cccgacacac cgcgtaatcc gttccgtggc gtgaagctgt
    17521 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17581 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17641 aacagcctga agcggcgaag cttaacgcta gcgtaaatta aaggcgctac gcgtaatgcg
    17701 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17761 ataacccacc tttacgctgc gcacattggt aatcaggcgc ttatacata
//
LOCUS       DAZPJP010000071        17753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPJP010000071 DAZPJP010000000
VERSION     DAZPJP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.071"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHU9J_002205"
     CDS             <1..245
                     /locus_tag="ACHU9J_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1905733.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHU9J_002206"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHU9J_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1905734.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHU9J_002207"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHU9J_002207"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1905735.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHU9J_002208"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHU9J_002208"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR1905736.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNLQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHU9J_002209"
     CDS             3423..4061
                     /locus_tag="ACHU9J_002209"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1905737.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHU9J_002210"
     CDS             4066..4842
                     /locus_tag="ACHU9J_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1905738.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHU9J_002211"
     CDS             4931..6295
                     /locus_tag="ACHU9J_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1905739.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHU9J_002212"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHU9J_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1905740.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHU9J_002213"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHU9J_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1905741.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHU9J_002214"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHU9J_002214"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1905742.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHU9J_002215"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHU9J_002215"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1905743.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHU9J_002216"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHU9J_002216"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1905744.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHU9J_002217"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHU9J_002217"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1905745.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHU9J_002218"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHU9J_002218"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1905746.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHU9J_002219"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHU9J_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1905747.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHU9J_002220"
     CDS             complement(12844..13167)
                     /locus_tag="ACHU9J_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1905748.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHU9J_002221"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHU9J_002221"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1905749.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHU9J_002222"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHU9J_002222"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1905750.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHU9J_002223"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHU9J_002223"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1905751.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHU9J_002224"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHU9J_002224"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1905752.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         4163 a         4760 c         4498 g         4332 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaacctcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acctagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat aca
//
LOCUS       DAZPJP010000072        17490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPJP010000072 DAZPJP010000000
VERSION     DAZPJP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.072"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..128)
                     /locus_tag="ACHU9J_002225"
     CDS             complement(<1..128)
                     /locus_tag="ACHU9J_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR1905753.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEP"
     gene            complement(101..199)
                     /locus_tag="ACHU9J_002226"
                     /pseudo
     CDS             complement(101..199)
                     /locus_tag="ACHU9J_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(188..427)
                     /locus_tag="ACHU9J_002227"
                     /pseudo
     CDS             complement(188..427)
                     /locus_tag="ACHU9J_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            553..1395
                     /locus_tag="ACHU9J_002228"
     CDS             553..1395
                     /locus_tag="ACHU9J_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905754.1"
                     /translation="MTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            1397..1924
                     /locus_tag="ACHU9J_002229"
     CDS             1397..1924
                     /locus_tag="ACHU9J_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1905755.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1953..2486)
                     /locus_tag="ACHU9J_002230"
     CDS             complement(1953..2486)
                     /locus_tag="ACHU9J_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1905756.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2489..2635)
                     /locus_tag="ACHU9J_002231"
     CDS             complement(2489..2635)
                     /locus_tag="ACHU9J_002231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905757.1"
                     /translation="MKKGVMYEKSGHVITGLGIIGEVDGDDPAVFRPIQKLINGTWYN
                     VSQV"
     gene            complement(2639..5266)
                     /locus_tag="ACHU9J_002232"
     CDS             complement(2639..5266)
                     /locus_tag="ACHU9J_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1905758.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLREL
                     GTSGEKIPLLSTANTWTNRQTFSGGLSGELSGNASTAAKLKTARKISNVAFDGSSDIT
                     LKASHVGAFALGKTGSTVANDKAVGWNWSSGAYNATISGASTLIIHFYMGEGSCPAAQ
                     FRINYKNGGIFYRSARDGYGFEADWSEFYTTRKPSAGDVGALPLSGGQLNGALGIGTS
                     SALGGNSIVLGDNDTGFKQKGDGNLDVYANNVHVMRFVSGSIQSNKTINITGRVNPSD
                     YGNFDSRYVRDVRLGTRVV"
     gene            complement(5418..6017)
                     /locus_tag="ACHU9J_002233"
     CDS             complement(5418..6017)
                     /locus_tag="ACHU9J_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1905759.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(6085..9536)
                     /locus_tag="ACHU9J_002234"
                     /pseudo
     CDS             complement(6085..9536)
                     /locus_tag="ACHU9J_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(9880..10560)
                     /locus_tag="ACHU9J_002235"
     CDS             complement(9880..10560)
                     /locus_tag="ACHU9J_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1905760.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(10458..11201)
                     /locus_tag="ACHU9J_002236"
     CDS             complement(10458..11201)
                     /locus_tag="ACHU9J_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1905761.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(11212..11910)
                     /locus_tag="ACHU9J_002237"
     CDS             complement(11212..11910)
                     /locus_tag="ACHU9J_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1905762.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(11910..12239)
                     /locus_tag="ACHU9J_002238"
     CDS             complement(11910..12239)
                     /locus_tag="ACHU9J_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905763.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(12236..14809)
                     /locus_tag="ACHU9J_002239"
     CDS             complement(12236..14809)
                     /locus_tag="ACHU9J_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1905764.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(14790..15203)
                     /locus_tag="ACHU9J_002240"
     CDS             complement(14790..15203)
                     /locus_tag="ACHU9J_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1905765.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(15230..15661)
                     /locus_tag="ACHU9J_002241"
     CDS             complement(15230..15661)
                     /locus_tag="ACHU9J_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1905766.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(15675..16427)
                     /locus_tag="ACHU9J_002242"
     CDS             complement(15675..16427)
                     /locus_tag="ACHU9J_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905767.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(16435..16830)
                     /locus_tag="ACHU9J_002243"
     CDS             complement(16435..16830)
                     /locus_tag="ACHU9J_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1905768.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(16827..17402)
                     /locus_tag="ACHU9J_002244"
     CDS             complement(16827..17402)
                     /locus_tag="ACHU9J_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1905769.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(17417..>17490)
                     /locus_tag="ACHU9J_002245"
     CDS             complement(17417..>17490)
                     /locus_tag="ACHU9J_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1905770.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         3781 a         5162 c         4245 g         4302 t
ORIGIN      
        1 ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc
       61 acgttcttgc ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg
      121 taggccactc aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact
      181 ttttctaggc ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca
      241 gttttcagac aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag
      301 gtcggcctgt ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa
      361 caagagcgtt tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa
      421 tcagcacgct gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc
      481 attatctaaa acctcggttt acaggaaacg gtaaacaagg ctggtaacgc cgttcagcgt
      541 tccggcgata aaatgaccgg agaactgaaa attggcacgg tgaatgcgct gcgaattttc
      601 aatgatacct tcggtctaat tttccgtcgt tcagaagatt ttcttcattt cattccgacg
      661 gctgaaggac aaggcgaaaa cggtaatatc ggcccattaa ggccattcgc tataaatctg
      721 agaacaggtg ctatatctgt cagccacggg gccaaaatta aaggaggtct agctattgga
      781 gctactgata acgcactggg tgaaaactcc attgttctgg gagataacga caccggattt
      841 aggcaagatg gagatggtat tattagcttc tattcaaatg gttcgcgcat cggacatatt
      901 gatgagttag gattacatct ttataaagat attgaatcta atggcagcaa ttttagatta
      961 aaaagtaatt accgccacca cattacattc gccaacgaag acggaagaat tcgtatgttt
     1021 ttgtggaaag ataacggtgg tgatggtgtt catattaata acggttcaga tggtggtggt
     1081 gatttcattt ttaaaacaga tgggggattt gcgctgggaa gtggtgcgca agttgcgtct
     1141 agtggcgata tttatggttc agtgtgggaa aacaactggt taagcacatg gctgcataat
     1201 catgtcgttc gggatattcg tcttggcagc attgaatata aaaacgtatg gcgcgactac
     1261 ggctttggcg atgcgtcagg ttatgtttta acagccgcaa ttaacagcaa tgcggatgat
     1321 attgtcgaca ctgttgccag aaggccaatt cagaaattga ttgggggaat atggtacaac
     1381 gtggggagtg tttaagatga tgcacctgaa aaatattaag gccgggaatc cgaaaacacc
     1441 ggaacaatac cagctaacta aaaaagcggg cgtggtatgg ctcttttgcg aagatggcaa
     1501 aaactggtat gaggaacaaa agaattttca gccagacacc ataaaaattg cttacgatga
     1561 gaacaacatc atcgttgctg tcagtaaaga cgtttcaact attaatcctg aagggctaag
     1621 catagttgaa gttcctgata ttacggcaaa tcgtcacgca gacaataacg gaaaatggat
     1681 gtttctggat ggtaaagtag taaagaggga atatacagaa caggaactgc aacaacaggc
     1741 cgaatcacaa aaggccgcat tgctttccga agctgaatca gtcatccagc cgctggaacg
     1801 cgctgtcagg ctgaatatgg caacagacga ggaacgcaca cgactggaag catgggaacg
     1861 ctacagtgtt ctggtcagcc gtgtggatac ggcaaatcct gaatggccac aaaagcctga
     1921 ataaaaatta aggcccgata gcgggccttc tctcattctg gttgttcggg aaacgttact
     1981 ggcaggctgg aggtgtctgt ggattcaacc ttctgcgcat agagcatcca ctcggttaat
     2041 ttttgtttat tctcatcgga aatgatgccc agctgtagct gagagtccca cagctgggtt
     2101 ttatccctga cgagctgcag caggctctgc ttttcatttt ccgcttgttg cctttgctct
     2161 tcctcggtat aagttcgctt tatcaccgca ccatctttga acatccagtt gcccgaaata
     2221 tcagcccggc gatttgctgt aatatcaggc aactcaacga cacttaaacc ttctggatta
     2281 attgctgata catccttgtc tatagcaata attattccct caactgtgta agccagtttt
     2341 attgtatcct cctgaaagtt ttttaattcc tcatgccaat tttttccatc ttcggaccat
     2401 aaccagataa catcaaaatt ctttgttagc tggtattgtt caacggtttt tggattacct
     2461 gcgacaatgt tttttaaatg ctgcatgatt atacctgcga tacgttatac catgttccgt
     2521 taattaactt ttgtattggt ctgaatactg ccggatcatc accatcgact tcaccgataa
     2581 taccaagccc cgtgattaca tggcctgatt tttcatacat cacgcctttc ttcatggtct
     2641 aaacaactcg tgtgccaagt ctgacatctc tcacatagcg ggaatcaaag ttaccgtaat
     2701 ccgagggatt aacacgtccc gtaatattta tggttttatt actttgaatg cttccggaga
     2761 caaagcgcat aacatggacg ttattagcat aaacatccag attaccatcg cctttttgtt
     2821 taaagcccgt gtcattatca cccaatacaa tcgaattacc gccaagagca ctggatgttc
     2881 cgatacccag tgcaccattc agttgaccac cagataacgg cagtgcacca acatctcctg
     2941 ctgaaggttt tctggtggtg taaaattcgg accagtcggc ttcaaaacca taaccatcac
     3001 gggctgaacg ataaaaaata ccgccatttt tataattaat ccgaaactga gctgcaggac
     3061 aacttccttc tcccatataa aaatgaataa ttaacgttga tgcaccacta atagttgcgt
     3121 tataggctcc gctactccag ttccatccaa ctgctttatc attcgcaacg gtgcttcctg
     3181 ttttccctaa ggcaaacgca ccaacatgac ttgcttttaa tgtgatatcg gaggaaccat
     3241 caaaagccac attgcttatt ttcctggcag tttttaattt tgcagctgta gaagcattgc
     3301 cggatagttc accagaaagg ccaccgctga atgtttgtcg attagtccag gtattcgctg
     3361 tactgagtaa cggtattttc tccccgcttg tgccgagttc tcgtaaaccg aggtattcga
     3421 taacggcagc aacggtcgat ttcgcaagaa tatcccgccc gacttttgtc agggttgcca
     3481 ggctggcgac atcattcccc gtaaaatacg gaaacttgcc tgccgcagta gtaagtccgg
     3541 ccagcgccgt cagggtggca tctttcggtt gcttacccgc aagcgcgtta gtcatggtgg
     3601 tcgcaaaatt cgggtcgttg cccagcgccg cagccagctc gttcagcgtg ttcagtgcat
     3661 caggtgacga atctacaagg gcggcaatcg cagccataac gaaagccgtg cttgcgattt
     3721 gggtattatt cgttccctgt cgcgcagttg gtgttgttgg cgttccggtc agtgcaggac
     3781 tatttaaggg cgctttcttg ttcgtttcac ccattaccgc cttaaccgct tttggcgttg
     3841 ccgccagtga ctcggaagtg ctgttggtcg cactgctgag ctgtactatc ccctttttcg
     3901 tcgtgctcgc atcctccagc gccacggcgg atgcaatatc ctctgcccgt tttgctgctg
     3961 tctcggcgcg cgttgccgcg gattcagcag caactttgct ctgagatgca gccgtcgcac
     4021 tgcctgccgc ctctgatgct ttcgttgttg ctgtcgtggc actacctttc gctgctgacg
     4081 cttgtctggt cgcctcatct tttgaagcag acgcagatga tgccgatgac gccgctgaac
     4141 tggctgacga tgcggctgct gccttagagg aagcagcatt gtctgctgaa gtctttgcat
     4201 ttgtttcaga ggtttttgct gcagaagcag acctcgctgc tgcagtggct tgctcagtgg
     4261 ctttgccagc cttcgttgtg gctgttgaag cggatgatgc ggcgctttct gccgattttc
     4321 cggcggcggt ggcactggct gaggcctgcc cggcacttgt tgacgcggca ctggcagata
     4381 atgcagccgc tgtttttgaa cctgccgcag ctgaggcgct ctgtcccgct gctgtttcag
     4441 aagacttagc gttcgtctcg gacgtttttg ccgccttcgc ggaatttcct gccgccgttg
     4501 ccgaggaagc tgcactactg gcacttgatg atgcattcgt ttctgaagat ttcgctgcct
     4561 cttttgaggc cgccgcaccc cgtgccgagg tggcagcttc tgacgccttc gtggtcgctg
     4621 tggatgcaga agtggctgcc gatttttgtg atgctgcggc attcgtttct gacgttttcg
     4681 cggcactggc gctggtagct gccgcgcttt ttgatgactc tgcagcagca ctttttgctg
     4741 cttcacgggc ctttgtcgat gccgttcctg cgctggaaga cgctgactga gccgacgacg
     4801 cggcctgtcc ggctgacgtg ctggcggcac gtgctgaggc tgcagcatcg gttgcatgag
     4861 ttgccgcctc gctggctgat tttttcgcgg ctgccgtatt ctgtgcaacc gcggaggcgt
     4921 tacgtgacac ctcttccacc atctgctcaa agcggcgcag tgcctccggt cggacatcat
     4981 cctccgtcat ggcaccgaga aaatcattca gcgtacctgg tctggaacct tcatagacgg
     5041 taatggtccc ggcatgtgaa ggcggaaaac cttcaaccag cagggtgacg ctgtactgac
     5101 catgctcaac atccatgctg taacgtccgg cttcatccgg attttcagag gccaccgtgt
     5161 tcaccaccat cgtgctgctg gttcgtctgg ccttcagcac aatggtgcag ttctgtactg
     5221 gttttcctgt gccatcttta agcacgccag aaatttttac tgtcatactt ttccaccaat
     5281 aaaaaaagcc cgcagcagtg acgccacggg cttcaggaca gtgtaacttt acgtttcctc
     5341 aaacgcagtt caccccataa ggtggatgaa cctgcgtatc ataacaatat ttacagaaga
     5401 taaatcggcg tctgttgtca gatacggtat ccgataccaa caataaatgc atccgttcgc
     5461 cagtcaccac taccggaacc ttcataagca aggtcaatgg tcacggattc ggtcgggtta
     5521 aactgcacgc cagcccccca cgccagagac gtgttgctgt ggcgaccgtc atcacttccg
     5581 gtcagcacat cgtgcgtttt ccccttgttg tcagttacgc ggagataatc cccggagaaa
     5641 gtcgacacac ggctgtaagc cacacccacc atcgcatacg cgctgaacca ttcattcacg
     5701 cgtacagacg gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag
     5761 cgggtatcgc tgtagtgcgt tttttgctca tcctcagcat tggcataact gaaggacgta
     5821 atcagcccca gcgcgtccgt aaactcataa cggtatttca cgttaatccc gttcagatta
     5881 tcgctgccgg gagcgttcgt acgggcatga agataccccg cgctcagtgt ggactgatgt
     5941 tcagacgccc atgcaggcgc accggatacg gacagacaga tggctgcgga caaaatggct
     6001 gcacaaactt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc gccatttctg
     6061 gcgcccgtat atgggttata aaattcagct gatactgatg cctgcggtgg ctttcttcat
     6121 caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagttattca ccagccatgc
     6181 tgacaccgaa aactccagcg tcatgtggcc gcgaccagct ggcatatcaa taacaccact
     6241 gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccgt ttttccgtac
     6301 tatcaggcgg catgacgaat aaatatcgtt attctcccgc tcatgtttag cgccgctgaa
     6361 tgccaccgcc ggaataacaa tttgcctgtc aaaaggctga tcgtcataaa tcctgacggt
     6421 aatggtccct gatggccacc gctccggtgc ccgggagtcc cgcggaaaag ccttacccac
     6481 tgttttgact atatcgcctt caatctggtt ggctgacagt ttccccttaa tctgacagtt
     6541 ctcattaatt gtgacattgt tgagcgtccc ggcgttcgca ttcacactgc cactgatatc
     6601 tgcattttta gcggtcagtt ttccgtccgg tgtcagggaa aatgccggag gattaccgcc
     6661 gctggtaatg gtgggagccg tcaggcgttt caggaacacg tcgttcatga atatctgatc
     6721 gccctgacca acaaacatcg gctttgtgtt gccattcgca ggattaatca tcgcaatcct
     6781 gtctgccgcc agcagcacct gactctgcat tcctgctggc gtattctcaa taccggcacc
     6841 gatacccgca atataaaggc gtccgtcctt catctgttgc agcttcacag cccacatgct
     6901 gttcagatta ttatttgtat caacctgaac cttctgtatc tgctggattg ccgcactctg
     6961 gtctttcagt ttcttattga cagtctgcgt gatttcattg ctgacatccg taatggacgt
     7021 cctgatttca gccaggtcag gcgcaagctg accgttatca atctgcgtcc acagctcctg
     7081 agccagatgt gttttcccta tctctccttt gaaaaaatcc aagtaacctt ccgcatcatc
     7141 gctcgcccga ccgacagcct ccacgaatgc cgatttgcca acggtgttca cactgcgaac
     7201 gtaaaaataa taatcatggc ccggtttgat attgatactg gcggctatcc agtacagcgc
     7261 cgtgccaaga tagcgggcgc tggtttcaac ctgcctgata tccgcaatcc gcttttccga
     7321 gaaccagaac tcaaactgta ccgtcgggtc ataaacggca agatgcggcg ttgcggttat
     7381 ctgaaaatag cccggcgtca gctcaatccg cgacggtgct gccggtgcgg caatccggaa
     7441 ggtggtggtg gcaggttcac cctgctggcc atagctgttt atcgcccgca ccgtcagggt
     7501 gtattccccg agcggcaggc cgctgaaacg gtgctccgtg tctgcggtga tggcggtggt
     7561 caccagtctg gcatccgttc ccttaccact ggtcaggcgc agactgaagc gcacaccctt
     7621 caccacccgc ggcgtgtccc atttcgcctg cgccagatac tggccgtcag gtgctgcact
     7681 gccggtggga tgacgctgtt cagggaacct gactgcggct caaagcgggc accgttatcc
     7741 acgatggctt ctttttccag tacgtgctgc accgccgtga tggcaaaggt gccgtccgtg
     7801 ttttcccgga cggagacaca gcggaacagg agacggcgca gtgacggcag ggagagtccc
     7861 cacaccccgt atgtctccac accatcaggc agggtactga cctgtatccg gtccggcgcg
     7921 gggtgttcgg tgatgtccac actcaccggc ttaccgctgc cgttaatcag gttcaccgtg
     7981 gcggcaccgg tctccggaag tgtcacttca cggtccagcg tcagagtgcg ggtggcagca
     8041 tcaatggaca ggacacgtcc gccggtcagg gtcccggcat agtcgttatc acagatttca
     8101 atgatgtcac cgggtgtgtg ccgcagcccc tgagacccga gcgtgaaatc caccgtctgc
     8161 gtttccagca gttcggtctt tatcacccac agtccggcac ggtgggcctg accgcggctg
     8221 gtacagccga acgcgtccat cttcagcagg ttgcgtccgt agcgcagtat ggcttccggg
     8281 tcttccacca gttccgtgga ggtctgccag ccgttctgcg ggtcggtata attcacctcc
     8341 accgccgtgt gccggtcctt cagggcactg aagctgtagc ggaaacccac gccgttatca
     8401 tccaccacca catcgctgtt ggtgtacggc cacaccacat ccgacgggcg gtcctgaaca
     8461 aacgtcagcg tctggccgtt ccataccggc atacagcgca tcgccgagca gaaatcactg
     8521 agcacgtccc atgccttacg ctgttgtgcc aggtacgcat tgaaggtcat ccgcggctcg
     8581 gtctccccga aactatccgg gaccgtctgg tcgcagtact gcccgatggc atacagcgcc
     8641 cacttgtcca catccgccgc ccccagacgt tttcccatgc cgtagcgcgg gtgagtcagc
     8701 atgtcccaca gacaccaggc cggattgttg ctgtatgccg gtttcaggct accgtcccag
     8761 ataccactgt acgtgcgttt ttccgggtca tagtttgacg gcacctggat gatgcgaccg
     8821 cggatatggt agttcaccgt catctgctgg ccgccgaact gctccgcatc cacctgcagc
     8881 cccacaatgg ccgtgttcgg gtagcactgt ttcacatcga tgatttcggt gtatgacgac
     8941 cacagcgtct tattctgcag ctggtccgtg gtgctgtccg ctgtctcccg gaccatccgg
     9001 atgttaaaag gacggggagg cagattatcc agaatcaccg acgccagaaa ctgcgaggtg
     9061 gtcttgccgt taatggtgac atccttttct gtcacccagt taccgttacg ctgtaactga
     9121 atcagcaggc ggacggatgt cgggtttcgg tcaccctttg acgtggtctg caccagtgac
     9181 tgcaccccga aggtaacccg caggcggtca atgttcgcgg acgtaatggt gcgcgtcacc
     9241 ggttttgcct tcgtcacttc cacgcccagt ccggtttcag caccggagga ctcaaagcct
     9301 tccggtggtg tctgctcctg ctccccggcg cgtcagaccg ccgtcacacc gtgtatcacg
     9361 ggattaccgt ccgtgtccgt cagcggggtt ttgttcacca ggatactctg cagtcccttc
     9421 accggacctt ctatcagtcc ctcaccaatc gcatcaatca cgctcatcat ctgcgtggat
     9481 ttgagattat ccttcgcctc acgaggcgtg tgtgccttac cgccaccttt tcccatacag
     9541 ccttcccctg aataaattaa ccgccacttg ccattccgta cagaagtcgg atatccttcg
     9601 cccgaaaagc atgaaacaca tttctgccat gctaaagaga aaccccggta tcagcagata
     9661 ccggggtttt ctttcatgcc caccgataat cctgttggtt aaaaccggta atggcataaa
     9721 aattctgaat atcttcacat tttcacaaac tgactgtggc gcgtataatt tctctgcgtt
     9781 aatttttttg tcgtgatata agaataattc cttacactta atcttcgtaa ctctcccgca
     9841 gttcctgtcc gcgatcactg cgggattttt ttattctttt tacccctgcc gcccgataac
     9901 cacgaccttt ccgcccccgc cttcatcacg ggtgctgatg tcctgggata tacggcggga
     9961 gccaaccagc atttccccgt aaggcaccgg catcgggttc ccctgggcaa tcatgttatc
    10021 cagtgaggaa aagtacgtgt tctgtctgcc gttatccgtt gcgcggtaat ccggtgtttt
    10081 tgccttcggg gccagcatct gggccacacc gcccagaatc atgctggctc caagtgaaaa
    10141 cagcatcgtg gtggcagaaa aaccaccggc tgccagggct gaaccccata acgccattga
    10201 tgccccggca gtgaagaaag agcccacgat ggctgccgcc cccagcacaa tctgcagtcc
    10261 accctttccg gccccggcca gtcgcggcac aatgtggatg accgttccct cacccagctg
    10321 ttcgtgaaga cgggcgtaca ccgcctccgg tgccgtgtca taaccggcaa tacgtatctg
    10381 gtaccagcct tcgttcatct gacggcgaaa gcccggcatc tgcatcgaca gggcgcgaat
    10441 ggcttccgct gccgtgttca catacaggct gaggcggcgg ccaaatcgtt gtaaatcccc
    10501 gtgaaggcag atgcgtgcca gtggcggtga cgccagactg aatgcgttcg tcgttgccat
    10561 ttttcggaat acctctcccg tttactcagt tgttcaggca gatggtgaag cagctcaccg
    10621 ttgccgcagt aaatggcggc atggttcggt accgaagcac caaagcagca cagcagaata
    10681 tcgcccgcct gtgcagagga caggggcacc cggtaaaagc cggtgaccgc catattgtcc
    10741 aggtaaaggt tctgaccgtt gcgccaccag tcatcctcgc gatgaaaatc cggcatttca
    10801 gtccccgcca gatgataagc atcccggaac agcgtgtaac agtccgtcac cccgtgctca
    10861 aagcgccgtc ctgtcagatg tggcacacag cggaatttgt gaatgtcacc ccggcagacc
    10921 aaccaccagg gcagtgcgct ttttatctgc aaccgccggt cagcctcgct cagccagggc
    10981 agcccaccgg gatgactgtg gaccagtgcc acaatctccc cctgcatctc tgcccgcagc
    11041 cagtcttccg gtgcgatacg aaaatacgcc tccggctctg cggaaatatt cacacaaggg
    11101 atataccgct ccccctccgg cgtgcttatc acgaagccgc acgactccgc aggcgcacac
    11161 cgccgggcat gtgccagaat cgctgattca gtctgtgtca taaaccggga tttactgcga
    11221 aagtttatta atggaaagga aaccgccaaa attgccgaca ttcctgcgca gttcacaccc
    11281 gcgcatgcac ttgctgcatc tgtccttacg gatatccgtg gtggggttgt cgaactcatc
    11341 cgccaccgca ggaccgttat acccgcattc atctccccgg taatcccaca tacaggtgtt
    11401 cgccagcatg atgcgaccgg gaaacagcgc cccgtccgtc tcggtcggtg tagccagcac
    11461 aaacgaggcc gtcatggctg tcagctgcga catctgctcc accacccagc ggtcactcag
    11521 ctcctgctcc gggtccgcct ccggattgcc cgcaacgaaa ttcaccgcat ccagaaaacg
    11581 ggcatacacc cggcggcgga ccaccgtggc ccccaccaga ctctgcaggt cttccgccat
    11641 cccggtgacc agaccgaaca gattggacac cgtcagcgac ggtctggcac tgctgccccg
    11701 gccgttcatc tcaaagccgc tgccgtcaat cgggtatgcc tcatacttac gcccctgcca
    11761 ggtgaccggc tccccttttt cattcagctc attacagaaa aaataccgct caccaccctg
    11821 taccgtcagg tcgatttccc agagtaccac ccgcggtgac tgctctgact taaccgactc
    11881 gttcagactt tcttcgcgaa tatcctgcat cagttcacca cctgcttaaa ctccgcgctg
    11941 aactcaacgc gcaacatccc gacccgcgca gaccacccgg cacaggtcac ctttatctgc
    12001 cggtatgcat agggtggctt ccacaaaaat gccttccagc caccgtgctc tgccaggaac
    12061 gcttccagat gccgggcctc ctcccgggtc acggaaagcg tcaccctgta tgttttcagg
    12121 tcagcattca gccctgccgc catacgctgt gagtacccgt caccaaaacg cacttcacgc
    12181 accgatggct gcgagttcac ctccatatcc ggcttcactt tccagcgaaa ggttttcatc
    12241 caccgctccc tgataacata ccgccatcac gcaactgcag ccggagttca tcctgtgccc
    12301 ccttgcgggc catctcatac accgctttca tcagctgcgg ccctgcccgc ccgttggggc
    12361 cgtcgttctg aatcaccacg tgattgttct gattaaaatt aatgccttcc gcccgccgca
    12421 tctgcgccgg acttccggca ccgccgacat aaccaccttc cgcatagccc cgcatcaggc
    12481 ggtacagatt gccgacacca atccggctgg tcgcctcctt cgtgaagaca aactccccgc
    12541 gatgaacaat ccccgcaggt tcatatttac cccccgtccc cgtaaatccc ccggtcgcga
    12601 aatggaagtt cgccgccgca gcctgaatgg ccgtccccgt ggaggcagac gcaccaccac
    12661 cgaaagcacc gccaatggcg ctgccgatac tcccgactat ccccaccatc gcctgcttca
    12721 gaaaaatctc tgtcagcatg gacagcacag aacgggtgaa accacgccag ttctgttcgc
    12781 tgccgatcag catcgctgcc atattctgtg caataccgtc aaaggtctgc gtggccgcgt
    12841 ttttaacctg cgaaaaactg tccgtcgcac tttccgccca ctcgccccag ccggacttca
    12901 tcccggccat ccagcttcca cgaagctgct cctccgcaga ccaggtgttc ttcagtgcag
    12961 atgtggcctt cgccagcgca gccggattat caccgtacac ctcacgaagg cgctgctctt
    13021 ccgactcccg ctgcgcctga cggtcggtga gtccgcgggc ttttgcgctg attgccgcct
    13081 gcttcgcgct ctgctgctgt tcaaaccgcg ccgcctgctg tgccagctca ttcagccgtt
    13141 tctggtgttc aatcttgtcg cccagctcag ccagctggcg tttgtactcc agcgtttctt
    13201 tctcatgggc cagcagggat ttttcctgct cagataactg ccgtttcgtg gcggcctctt
    13261 tcaggaccac atactgattt tccgctttcc ataaatcacg gcgctgctgg ctgatttttt
    13321 cattcgcacc gctgtgcttc tccagcgtcc tgagctcagt ttcaagcgcc agcagggctg
    13381 catgcgcctg gtcttcctgg cgctcaccgg cagacacctt cacaccggac ggctttttct
    13441 gcgtcgactc ataatccttt tttgccgacg ccatcagcgt gttgtaatcc gcctgcagga
    13501 ttttcccgtc tttcagggcc ttattcagct cttcctgccg ggcggtatat ttctccagtg
    13561 gcgtcagcag gcgctcatac gccttctgcg cctctccggt atacttcagc tgtgacgact
    13621 cacgctcagc cctgtccctt gccgccagtt caccggcttt ttccatatcc gactgcagcg
    13681 ttgccgctgc cagacccaga cgggcatttt cccggtcatc ccatgcgccc tgaaggttgg
    13741 cccggaaaga ggaggtttta ccgcggcgct ggctccgact ctggtaccac tgccattttt
    13801 tatccgcctc atcaaatgcc ttctgcgcac tggcgagcat atccgctgag gattccggac
    13861 gaccgatatc cagaatggca tcccacatcg atttgaatgc cttccctgtt ttatccgccc
    13921 aggtctccag tgttcccatg ttttctttca ggcgacgggt ctgctcatca aagcctttcg
    13981 tggcgatatc gttcgccgcc tgcaatgccc cggcctcgtc tccggaacgc tgcagctgtg
    14041 caacatacgc aatctgctct gccgtcacgt tacggaactg gcgcgccatc gccatcagtc
    14101 ccgacgtcgg gtcagtggtc agcttcccga aggcttcagc gactttatcc acctccacac
    14161 cggatgcaga cgcaaaacgc gcgacactct ggttgatggc atcaaactgt tcaccaccac
    14221 gcacaccggc attcaccagg gctgccagtg actctctcgc ctggttaaac gtcagccctg
    14281 ctgcctgccc ggctcttgag agagtcagca tacgatcggc agtcagtccg gactgattac
    14341 cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atctccctgg taccaggcgt
    14401 acaccagtgc acctgtcgcc accgccagcg aggtgacccc gaccatcggc agggtgatcg
    14461 catcggcaag ccccctgaac atggggatca tcccgccgaa ggagtccttc acctgaccgc
    14521 cctgttgcag caggatcagc cagggattct gaccaccggc aagctgcgtg gcgatatccg
    14581 taaactgtgc gggcagggtt cgcatggccg ctttatactg cccgacggaa atcccggctt
    14641 tttgtgcagc cagcgcctgg cggctcaggc cctgttcaac agcactggcg gtttttctgg
    14701 cgtcggtatc cagacctgaa aaatgacgcc ttacccggct catctgctca tcgaaacgga
    14761 cagcatccag actcaggtca ataacaagat caccaaccgg ctgggacata tctcacacct
    14821 cccggaatcc ccgctgaagc catcattaat gcggcatcat ccaccatgac atccgccaca
    14881 tccgcagacg ataaaatatc gcgccctccg tccccaccga accggacgcc tccggcaagt
    14941 cctgccgctt tctgcatcag cattttgtcc tcatccggcc tctccacctg ctcttcctca
    15001 tgccggggga caagcagact gaaatcagag ggatgcatat ccggatcgca aaaaaacagg
    15061 ctgagtacag cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc ctgaaaataa
    15121 tgcgtgcggt aaaaacggcg ccagtcggca tattcggtgg atgtcatccc ggcaagcatg
    15181 gcgcgccagt cgggtctccc catctcacgc gccagtctga gggcaaagtt cagctcgccg
    15241 tcgaagactt tcccgcagaa aaatcatcat cagtcagcgt gttatttttc gccacttcag
    15301 taatatcagt atccggacga acagcttcga tcatcccgga caggcacaac accacgtctt
    15361 ccgcccgggc aatggcatcg gcaggccagg tggtgagcac ttcctgctct atcttcatca
    15421 cggcctcatt cattgacggt gactgcgttt tctgtggatg gttatgccac agggacatcg
    15481 ccaccagaaa cgcgccggtt ctgacgagat cttccacgct tacctgcagg ttgccgctgg
    15541 attctgcctg ttctgcacgc cgtttcagga gggcaagatg ctcgatacgc tgcagcgcag
    15601 acaattcgga aagcgtgaca gacacaccgt tatattcaaa ttgttctgtt ttcagaaaca
    15661 tgtattacct ccgtttaccc tgcagcgccc gcttcagtaa cggtgacttc agccactgcg
    15721 gcgaactgac catttccgct caccacaggg atctgcacct tacctgtcgc cacgccgttt
    15781 accgtaattg tcatatcttt cacactaatg gtggctttcg acggatcggc ggaaaccgct
    15841 ctgaacgtct tgtcggttgc actttccggc tcaaaagaaa ccgtcagggt ggttgttttc
    15901 ccttttgcca ccgtaccgga tgtcggcgtc accttaatcg cactgaccgg cgtaattttg
    15961 ctgcgttctt ccgctacaga aggtttaccc acgttagtga ctttcaccgt gcgggtgatc
    16021 acttctttcg ccgtcacggc cttaccgata ctgctgaccc agccacgaaa cacatccacc
    16081 gtgccattcg gaaaacggat tttataggcc cggacatcgc cgctttcaaa ccagcctata
    16141 agccctttct ggccttcctc tcccggtttc caggccagcg taaaactggt atcacctgca
    16201 gatttctgcc cctgcccggt cgcgctccag tccgcgtctt catcatccag gtagttatca
    16261 tcataggatt cagccgtcat ctcgcccggc gtcagatcct tcaccttagc cagtcgctgc
    16321 cagtcatcgt ctgacaacgg gtttgcataa gcatcagcct tgccgttgta aacccacaga
    16381 gtggtaccgg caccttttac cggctccagg ggatttggtg ttgccatatc gtcctcacat
    16441 ctcgtatgta atggaataag tcagatccgc agagctccat aacgccatat cgtcatcacg
    16501 acgatactca tagccctgcg taaccatcgt ggtaatcagt cctgccagtg cagggatcgc
    16561 agtcatcgcc ggataaatcc ggctttccat ccactgatcg agctccgaat caggtacctg
    16621 tgccggtaaa aacacctcaa tatgcagtgt ggcccgccag gtatctgcat ccagctcttc
    16681 accggtatac tctgcatccg tcagataaac cgcgatcgca ggaaaatcct cttcgtcaaa
    16741 aacaacgggg cgaccatcaa acagcgtcgc cccgtgttca tgctgctcga gtgcatccag
    16801 cactgcggca cgaatgtcag tgtgtttcat cgttttatcg caatcctcag ttgttgtttc
    16861 agcgcgtatg ccagttcttt aggcaggcgt tcacgccgga tacggtcaac attctcatca
    16921 aatgcctgtt tcagtggggc cgccatcggg attttcacca cctgaatggg aaggcgatta
    16981 cgctttttcc ttcccttgtc gtcattgccc tcctcatatc tggcctgggg aagacgttgc
    17041 ataacatgcc agcgcccatt atttaatcgc tggataaatg cccgctgata acgatgctga
    17101 ccggctttga gtatgctgtt cggacgacgc cccagcattc tgatccccag cttaatcaca
    17161 gggagatcac cgcggttaac gataattctg gcattcggat ttctgaccgt cgcccgtttc
    17221 agtctggacc gttcctttac cagtttccgt ctcaccctgg tttcccgggc aacctgtgac
    17281 gaagactgat taatcgccgt tgtggccacg cggttaatgg tcattgcaga agcagccgga
    17341 atggcgtttt tacgaacccg gttcagattg tcaatcgcct gatcaagccc ttttatcgcc
    17401 ataattcacc ctgcgtttat cgtcgccggt taactgcggg tggttgccca cggttgagcc
    17461 agagataaca gctgcccccg tcatccggag
//
LOCUS       DAZPJP010000073        17448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPJP010000073 DAZPJP010000000
VERSION     DAZPJP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.073"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHU9J_002246"
     CDS             complement(<1..502)
                     /locus_tag="ACHU9J_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1905771.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHU9J_002247"
     tRNA            complement(688..772)
                     /locus_tag="ACHU9J_002247"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHU9J_002248"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHU9J_002248"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1905772.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHU9J_002249"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHU9J_002249"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1905773.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="ACHU9J_002250"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="ACHU9J_002250"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR1905774.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="ACHU9J_002251"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="ACHU9J_002251"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR1905775.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="ACHU9J_002252"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="ACHU9J_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR1905776.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="ACHU9J_002253"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="ACHU9J_002253"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR1905777.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="ACHU9J_002254"
     CDS             7117..8619
                     /locus_tag="ACHU9J_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1905778.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHU9J_002255"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHU9J_002255"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1905779.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHU9J_002256"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHU9J_002256"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1905780.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHU9J_002257"
     CDS             complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHU9J_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1905781.1"
                     /translation="MSIIILATISTTQENK"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="ACHU9J_002258"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="ACHU9J_002258"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1905782.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="ACHU9J_002259"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="ACHU9J_002259"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1905783.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="ACHU9J_002260"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="ACHU9J_002260"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1905784.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="ACHU9J_002261"
     CDS             complement(16186..17382)
                     /locus_tag="ACHU9J_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1905785.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4175 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccacttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac acgctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZPJP010000074        17348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPJP010000074 DAZPJP010000000
VERSION     DAZPJP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.074"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            4..981
                     /gene="ybeQ"
                     /locus_tag="ACHU9J_002262"
     CDS             4..981
                     /gene="ybeQ"
                     /locus_tag="ACHU9J_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1905786.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFMKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1051..1533)
                     /locus_tag="ACHU9J_002263"
     CDS             complement(1051..1533)
                     /locus_tag="ACHU9J_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1905787.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1768..4350
                     /gene="leuS"
                     /locus_tag="ACHU9J_002264"
     CDS             1768..4350
                     /gene="leuS"
                     /locus_tag="ACHU9J_002264"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1905788.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4365..4946
                     /gene="lptE"
                     /locus_tag="ACHU9J_002265"
     CDS             4365..4946
                     /gene="lptE"
                     /locus_tag="ACHU9J_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1905789.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4946..5977
                     /gene="holA"
                     /locus_tag="ACHU9J_002266"
     CDS             4946..5977
                     /gene="holA"
                     /locus_tag="ACHU9J_002266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1905790.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            5979..6620
                     /gene="nadD"
                     /locus_tag="ACHU9J_002267"
     CDS             5979..6620
                     /gene="nadD"
                     /locus_tag="ACHU9J_002267"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1905791.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6644..7255
                     /locus_tag="ACHU9J_002268"
                     /pseudo
     CDS             6644..7255
                     /locus_tag="ACHU9J_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7515..7832
                     /gene="rsfS"
                     /locus_tag="ACHU9J_002269"
     CDS             7515..7832
                     /gene="rsfS"
                     /locus_tag="ACHU9J_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1905792.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7836..8303
                     /gene="rlmH"
                     /locus_tag="ACHU9J_002270"
     CDS             7836..8303
                     /gene="rlmH"
                     /locus_tag="ACHU9J_002270"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1905793.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8334..10235
                     /gene="mrdA"
                     /locus_tag="ACHU9J_002271"
     CDS             8334..10235
                     /gene="mrdA"
                     /locus_tag="ACHU9J_002271"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1905794.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10238..11350
                     /gene="mrdB"
                     /locus_tag="ACHU9J_002272"
                     /gene_synonym="rodA"
     CDS             10238..11350
                     /gene="mrdB"
                     /locus_tag="ACHU9J_002272"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1905795.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11361..12449
                     /gene="rlpA"
                     /locus_tag="ACHU9J_002273"
     CDS             11361..12449
                     /gene="rlpA"
                     /locus_tag="ACHU9J_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1905796.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12588..13799
                     /gene="dacA"
                     /locus_tag="ACHU9J_002274"
     CDS             12588..13799
                     /gene="dacA"
                     /locus_tag="ACHU9J_002274"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1905797.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13909..14172
                     /gene="ybeD"
                     /locus_tag="ACHU9J_002275"
     CDS             13909..14172
                     /gene="ybeD"
                     /locus_tag="ACHU9J_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1905798.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14273..14914
                     /gene="lipB"
                     /locus_tag="ACHU9J_002276"
     CDS             14273..14914
                     /gene="lipB"
                     /locus_tag="ACHU9J_002276"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1905799.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15174..16127
                     /locus_tag="ACHU9J_002277"
     CDS             15174..16127
                     /locus_tag="ACHU9J_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1905800.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16336..17301
                     /gene="lipA"
                     /locus_tag="ACHU9J_002278"
     CDS             16336..17301
                     /gene="lipA"
                     /locus_tag="ACHU9J_002278"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1905801.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4324 a         4385 c         4456 g         4183 t
ORIGIN      
        1 ataatggttt ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt
       61 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
      121 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
      181 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
      241 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
      301 gcttccaacg cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg
      361 gttgtctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
      421 tggatgtaca gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac
      481 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
      541 gacggaaaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
      601 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
      661 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
      721 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
      781 tatcaggctg cctttgaatg gtttatgaaa gccgctgaat gcaatgacgc cactgcctgg
      841 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
      901 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
      961 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
     1021 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     1081 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     1141 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     1201 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatatc
     1261 tgccagctcc tgccataagc tttctttaat cacccgcata aagacgctgt cagattcttc
     1321 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt
     1381 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     1441 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac
     1501 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     1561 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     1621 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     1681 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
     1741 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
     1801 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
     1861 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
     1921 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
     1981 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
     2041 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
     2101 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
     2161 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
     2221 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
     2281 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
     2341 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
     2401 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
     2461 atcaccttca acgttaacga ctatgacaac atgctgaccg tttacactac ccgcccggac
     2521 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
     2581 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
     2641 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
     2701 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
     2761 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
     2821 tacggcctga acatcaaacc agttatcctg gctgctgacg gctctgagcc agatctttct
     2881 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac
     2941 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
     3001 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
     3061 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
     3121 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
     3181 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
     3241 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
     3301 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
     3361 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
     3421 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
     3481 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
     3541 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
     3601 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
     3661 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
     3721 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
     3781 cgtgtctgga aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
     3841 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
     3901 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
     3961 ctgatgaaca aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag
     4021 gaagcgctgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
     4081 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
     4141 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
     4201 gccaaaatca ccgttctggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
     4261 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca
     4321 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
     4381 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
     4441 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
     4501 cattaagccg tgcagtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
     4561 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
     4621 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
     4681 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcgt
     4741 tctttgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
     4801 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
     4861 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
     4921 gcgtctccac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
     4981 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
     5041 ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac
     5101 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
     5161 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
     5221 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
     5281 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
     5341 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
     5401 tgcgcgcgca aaacagctca acttagaact ggatgacgcg gcaaatcagg tgctctgcta
     5461 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
     5521 gccagacggc aaattgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
     5581 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cattgcatat
     5641 tcttcagcaa ctgcgtctgg aaggcagcga accggttatt ttgttgcgca cattacaacg
     5701 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
     5761 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
     5821 cttaagtcag acgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
     5881 caaacaagat tacggtcagt cagtgtgggc agagctgaaa gggttatctc ttctgttgtg
     5941 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
     6001 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
     6061 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
     6121 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
     6181 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
     6241 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
     6301 aggattcact gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
     6361 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
     6421 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
     6481 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
     6541 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtactgact tacattaacc
     6601 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
     6661 tcgtcatggt gaaacgcaag cgaatatcga tggtctttac agcggtcatg cgcccacccc
     6721 cctgaccgcg cgcggtattg agcaagcgca aaatctgcat acgctgctac atggtgtttc
     6781 ctttgatctg gttttatgca gtgaactgga acgggcacag cataccgcac gactggttct
     6841 cagtgaccgc cagctccccg tgcaaatcat ccctgaactc aacgaaatgt tttttggcga
     6901 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
     6961 gtgcaatgac tggcagcatg caatccccac gaacggtgaa ggatttcagg cattttcgca
     7021 acgtgtggaa cgctttatcg caaggcttag tgaatttcag cactatcaga atattttagt
     7081 cgtcagccat cagggtgtac tgagtctgtt aatcgcccgt ttaattggca tgcctgccga
     7141 ggctatgtgg cattttcgcg ttgactaggg gtgttggagc gccattgata tcaaccaaaa
     7201 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
     7261 ctgttttttt acgggtaagc cgcaacgacc attgacagac ccgggcaggc tgatattctc
     7321 cgcagccaga cttttttcgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
     7381 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
     7441 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
     7501 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
     7561 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
     7621 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
     7681 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
     7741 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
     7801 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
     7861 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
     7921 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
     7981 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
     8041 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
     8101 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
     8161 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcggcgctt accctccccc
     8221 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
     8281 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
     8341 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
     8401 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
     8461 aaattgttcg ctttaccgac taccagactc gctctaatga aaaccgcatt aagctggtgc
     8521 ctatcgcgcc cagccgcggc attatctacg accgtaatgg tatccctctg gccctcaacc
     8581 gcactatcta ccagatagaa atgatgccag agaaagtcga taacgtgcag caaacgctgg
     8641 acgctttgcg cagcgtggtt gatctgaccg atgacgatat tgctgcattc cgaaaagagc
     8701 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
     8761 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
     8821 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
     8881 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
     8941 atgatatcgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
     9001 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
     9061 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
     9121 aaacgctgct ggcgggtagc cgcgcagctg tggttgtcac cgatccgcgt acaggtgggg
     9181 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
     9241 gcaaagatta ttccgccttg ttgaacgccc cgaatacacc gctggtgaac cgcgccacac
     9301 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattgagcg
     9361 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctgccaggtt
     9421 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
     9481 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
     9541 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
     9601 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
     9661 ggtatcaggg tgacaccatt ccggttggta tcggtcaggg ttactggaca gcgaccccaa
     9721 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttcctcatt
     9781 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacagccg catgaaccgc
     9841 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
     9901 ctaaccgccc taacggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
     9961 cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
    10021 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    10081 cgcaagtggc tgtcgccatg attctggaga acggtggtgc gggtccggcg gttggtacac
    10141 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    10201 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    10261 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    10321 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    10381 taaaatcggc caaatcgcga tgggtctagt catcatggtg gtgatggcgc aaattcctcc
    10441 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    10501 ggtagatgct ttcggtgcca tctctaaagg tgctcaacgc tggctggacc tcggtattgt
    10561 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    10621 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    10681 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    10741 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    10801 agtgctggtg gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    10861 ccagcgcgta atgatgctcc tagacccgga atcagaccca ctcggcgcgg gctatcacat
    10921 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    10981 cactcagtca cagcttgaat ttctccccga acgccatacc gactttatct tcgcggtact
    11041 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    11101 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    11161 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    11221 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    11281 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    11341 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    11401 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    11461 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    11521 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    11581 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaaccc ggcagtaacc
    11641 tgacggcctc tggcgaagct ttcgatccga cacagctgac ggcggcccat ccaacgcttc
    11701 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    11761 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc actttctcgc gcggcagctg
    11821 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    11881 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    11941 tgccagcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    12001 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    12061 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta ctggaaggca
    12121 gcgaaccgac gcctgcgcca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    12181 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    12241 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    12301 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    12361 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    12421 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    12481 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    12541 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    12601 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    12661 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    12721 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    12781 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    12841 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    12901 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    12961 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    13021 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    13081 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    13141 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    13201 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    13261 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    13321 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    13381 tttctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    13441 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    13501 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
    13561 tgtacctgac cattccacgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    13621 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    13681 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    13741 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    13801 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc
    13861 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    13921 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    13981 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    14041 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    14101 actcatatcg agcaggttga aacactgtat gaagaactag gcaaaatcga tattgtccgc
    14161 atggttctgt aactcgcttc tccgttaccc ggcccctggt cgggtaactc ccctctcctc
    14221 gctgtgatat acttttcccc cacttttact cattctccac ggagatgcag ttttgtatca
    14281 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    14341 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    14401 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    14461 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca
    14521 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    14581 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    14641 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    14701 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    14761 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    14821 acccgaagcg acgactaata atattgcacc acgtttactg gaaaatattt tagcgctact
    14881 aaacaatcct gacttcgaat atattaccgc ttaattgcat acctcaatgg cccaatttac
    14941 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatactt
    15001 tctttatcac ttgcagaaat gcggtgctac gccttgctcc gtaactcacc ccattgatta
    15061 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    15121 tattgtaatt tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    15181 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    15241 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    15301 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    15361 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    15421 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    15481 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    15541 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    15601 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    15661 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    15721 ttctgttcac cgacaatatg gtgttaattt gccgcgaggg aaatccacta ctctctttag
    15781 aagatgacag agagactatc gacaacgctg cgcatgtact cctgttaccg gaagggcaaa
    15841 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    15901 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    15961 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    16021 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    16081 atcccatcct gcacggggta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    16141 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    16201 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    16261 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    16321 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    16381 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    16441 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    16501 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    16561 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    16621 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    16681 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    16741 gttgaccgtg atgacctgcg cgatggcggt gcccagcact ttgcggattg cattactgcc
    16801 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt
    16861 atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg
    16921 gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg
    16981 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    17041 gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    17101 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    17161 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    17221 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    17281 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    17341 gctttttg
//
LOCUS       DAZPJP010000075        16931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPJP010000075 DAZPJP010000000
VERSION     DAZPJP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.075"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            209..346
                     /locus_tag="ACHU9J_002279"
                     /pseudo
     CDS             209..346
                     /locus_tag="ACHU9J_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(454..1689)
                     /locus_tag="ACHU9J_002280"
     CDS             complement(454..1689)
                     /locus_tag="ACHU9J_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1905802.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1836..1912)
                     /locus_tag="ACHU9J_002281"
     tRNA            complement(1836..1912)
                     /locus_tag="ACHU9J_002281"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1876..1878),aa:Pro,seq:tgg)
     gene            complement(1955..2041)
                     /locus_tag="ACHU9J_002282"
     tRNA            complement(1955..2041)
                     /locus_tag="ACHU9J_002282"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2005..2007),aa:Leu,seq:cag)
     gene            complement(2062..2137)
                     /locus_tag="ACHU9J_002283"
     tRNA            complement(2062..2137)
                     /locus_tag="ACHU9J_002283"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2102..2104),aa:His,seq:gtg)
     gene            complement(2196..2272)
                     /locus_tag="ACHU9J_002284"
     tRNA            complement(2196..2272)
                     /locus_tag="ACHU9J_002284"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2236..2238),aa:Arg,seq:ccg)
     gene            complement(2375..3760)
                     /locus_tag="ACHU9J_002285"
     CDS             complement(2375..3760)
                     /locus_tag="ACHU9J_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1905803.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3951..4691)
                     /gene="wecG"
                     /locus_tag="ACHU9J_002286"
     CDS             complement(3951..4691)
                     /gene="wecG"
                     /locus_tag="ACHU9J_002286"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1905804.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4694..6046)
                     /gene="wzyE"
                     /locus_tag="ACHU9J_002287"
     CDS             complement(4694..6046)
                     /gene="wzyE"
                     /locus_tag="ACHU9J_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1905805.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6043..7122)
                     /gene="rffT"
                     /locus_tag="ACHU9J_002288"
                     /gene_synonym="wecF"
     CDS             complement(6043..7122)
                     /gene="rffT"
                     /locus_tag="ACHU9J_002288"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1905806.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7119..8369)
                     /gene="wzxE"
                     /locus_tag="ACHU9J_002289"
     CDS             complement(7119..8369)
                     /gene="wzxE"
                     /locus_tag="ACHU9J_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1905807.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8371..9501)
                     /gene="rffA"
                     /locus_tag="ACHU9J_002290"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8371..9501)
                     /gene="rffA"
                     /locus_tag="ACHU9J_002290"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1905808.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9506..10180)
                     /gene="rffC"
                     /locus_tag="ACHU9J_002291"
                     /gene_synonym="wecD"
     CDS             complement(9506..10180)
                     /gene="rffC"
                     /locus_tag="ACHU9J_002291"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1905809.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10158..11039)
                     /gene="rfbA"
                     /locus_tag="ACHU9J_002292"
     CDS             complement(10158..11039)
                     /gene="rfbA"
                     /locus_tag="ACHU9J_002292"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1905810.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11058..12125)
                     /gene="rffG"
                     /locus_tag="ACHU9J_002293"
     CDS             complement(11058..12125)
                     /gene="rffG"
                     /locus_tag="ACHU9J_002293"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1905811.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12125..13387)
                     /gene="wecC"
                     /locus_tag="ACHU9J_002294"
     CDS             complement(12125..13387)
                     /gene="wecC"
                     /locus_tag="ACHU9J_002294"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1905812.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13384..14514)
                     /gene="wecB"
                     /locus_tag="ACHU9J_002295"
     CDS             complement(13384..14514)
                     /gene="wecB"
                     /locus_tag="ACHU9J_002295"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1905813.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14570..15616)
                     /gene="wzzE"
                     /locus_tag="ACHU9J_002296"
     CDS             complement(14570..15616)
                     /gene="wzzE"
                     /locus_tag="ACHU9J_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1905814.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15628..16731)
                     /gene="wecA"
                     /locus_tag="ACHU9J_002297"
     CDS             complement(15628..16731)
                     /gene="wecA"
                     /locus_tag="ACHU9J_002297"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1905815.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16743..>16931)
                     /locus_tag="ACHU9J_002298"
     CDS             complement(16743..>16931)
                     /locus_tag="ACHU9J_002298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905816.1"
                     /translation="FCFYICNLNAALAMLGKFLEFAGVLCNLHIKWLIFAQDWWVRNG
                     LSLLNKGLRGYTSANNFL"
BASE COUNT         4361 a         4769 c         4105 g         3696 t
ORIGIN      
        1 cgcaatataa taaacctgcc ttacatatca actgcgccag aggtaggatt gaaaacgctc
       61 tcctgatttt ccaattcatt ttctggataa ataaataatt tatttttgtc actattattt
      121 atgtaatcat cctgtcaggg agagggatct caattatcaa tgcttaatta cgtcatcatt
      181 ttgatgacat gaaaccggag cattttgcat ggaattttcg ttttcaccca aacttcttgt
      241 tgttgctgtc gccgccgctc ttcctctcat ggccagcgca gcagataccc cgtcaactgc
      301 caccgcacgc aaaggctttg ccggatacga tcacccaaac cagtatccac agaacgctgc
      361 aatttattga atttgcacga tcatgtaggc cggataaggc gtttacgccg catccggcaa
      421 tcaaccgcag gcggccacaa aatggccgct tttttactta ttcaccacca gcaaatgtgc
      481 ctgcataatg tcactggccg ggcgaccgtg cgccagcaaa tcagccattg ctttaagata
      541 tggcggtaga tggcggaaat aacgctgata cccggcacac aaataattca gccccggttt
      601 gccgctggca tcgagcatga agcggtgttt cgggcagcct ccccagcacg cttttaacac
      661 gttacaactg cgacactgcg ccggtaattg cttaaattta tcttcaccaa acacctgctg
      721 ttgcggggaa tcgatcattt ctgcaattgt ttgctgatgc atattcccca gccgatattg
      781 cggataaaca tagtgatcgc aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg
      841 cccacaggtt ggctgatgat ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc
      901 ccattcgata ttcatcacga aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat
      961 cgccaccaga aattcaccaa attcatcggg gcgcaccgac cattccgtca gttccccctg
     1021 aatatcacca ggcgcatgca atttcagtcc atcgcgggca gctgtttcat cagccagacg
     1081 ctcgaccacc ggaataaact ggatgaattc gactcccgca tcgcacaaaa aatcataaac
     1141 ctgcaacggt tgctgcgcgc tggtgcggtt gacgcagacc agcacgttat agtcgacatg
     1201 atgtttttgc agcagcgtca gggcacgcat caccagctta tgcgttggtc tgccaccttt
     1261 ggtcacgcga tattgattgt ggatctcagc cggaccgtcc agcgataacc caacaagaaa
     1321 atgattttct gccagaaatg cacaccattt atcatcgagc agcacgccgt tagtctggaa
     1381 gctgttactt atcttcctgc cagcaccgta tttcgcctgt aactttacgg cacggcggta
     1441 aaaatccagc ccgagtaacg ttggttcgcc gccctgccag gtaaaagcca cttcgttttg
     1501 cggttcgctg gcagcgatat agtgacggac atacgcttcc agcgtgtcat cgtccatatg
     1561 cgtgactggc ttttcgcggt aaagggattg tttttcgaga taaaaacagt agtcacagtt
     1621 cagattacaa tcggaaccac tcggtttcgc catcacatga aaagcacgcg ttggaacctg
     1681 ttgcagcatg gtcactcctt gcctgaagtc atccttcaat acgttaaggg cgaaatcata
     1741 ccgtggggca atgagttatt acgggcgttt attgcgaagg gagggtgacg aaatgcacag
     1801 aaaacaaaaa accccgccga agcggggttc aaaattggtc ggcgagagag gattcgaacc
     1861 tccgacccac tggtcccaaa ccagttgcgc taccaagctg cgctactcgc cgaaatactg
     1921 ctttttgaat ttttagttca attctttaaa gtcgtggtgc gagggggggg acttgaaccc
     1981 ccacgtccgt aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg
     2041 cattgtcaca acttctaata atggggtggc taatgggatt cgaacccacg acaactggaa
     2101 tcacaatcca gggctctacc aactgagcta tagccaccac tacaaatctt gttacgcggt
     2161 attactacca ccgcagctca agcgccggga ctaaatggcg cgcccgacag gattcgaacc
     2221 tgagacctct gcctccggag ggcagcgctc tatccagctg agctacgggc gcttagcgcc
     2281 gttgcggggg tggataatac ggacttccca cccctctgtc tagtcccttt ttaaataaat
     2341 tgcgcgtttg gttacggttt gtgcgttttg ctgcttattc ctccagttta tgcgctttcc
     2401 cgtggcgatt aaggccaaaa actttataaa tcgccgtcac cgccagcata aagatgatgc
     2461 caacaaacag cgacatacgc gtatcttcat taaagtacat gccgatcaaa acgcaaatca
     2521 ggaatgccat tgttacgtag ttggcccacg ggaacaggat tgagcggaac ggatggctgg
     2581 caatcgccgc tttatgtgca cgccgaaaac gcagctggct tatcaatatc acaaaccatg
     2641 gcaccatccc cggaagcacg ctggcactgt agacgtagac aaacacacgc tgcggattgg
     2701 gaatgatgta gttcaggcat gagccaatta gcagaatagc aatagatact gccacacccg
     2761 caactggtac gccgtgacgg gaaactttcg ccatcgccgc cggtaactga cggtttttcg
     2821 ccagtgcgta gagcatacgt ccgcaactgt acatgccgct gttacagcca gagagcgcag
     2881 ccgtcagcac cacaaagttg ataatgcccg ccgctgcggt aataccgatt ttggcaaaag
     2941 tcagtacgaa cgggctgccg ttgctgccaa tttcattcca cgggaagatg gtgacgataa
     3001 cgaaaatcgc gcctacgtag aaaatcagga tccgccacag cactttgcct acagcactgc
     3061 gcagcgtcac ctgcggattc ttcgcttcac cggcagtaat gccaatcagc tccacgccct
     3121 ggtaggacgc caccacaata cacagcgcgg tcaggaaccc tttccagcca cccgcaaaga
     3181 aaccgccatg ctctgtgaga ttgctaaaac caatcgactg cccgccattg ccaaagccaa
     3241 agaaaatcac gcccaggcca atgacaatca tcacgataat cgtggtgact ttgatcatcg
     3301 cgaaccagaa ctcgatttcg ccgtacaacc gcaccgccgc cagattcgcc aacgccacca
     3361 gcgccaccgc gatcaatgca ggtatccact gcgccatctc cgggaaccag aactggacat
     3421 aaacgccaat ggcggtgatt tctgagatcc ccaccgccat ccacataaac cagtaagacc
     3481 aggcggtgag ataaccaaaa aacgggctca tataacgatg cgcataaacg gcgaacgaac
     3541 cggtaacagg ttcaaggaac aacatttcgc ccattgaacg catgatgaaa aagacgaaca
     3601 gcccggcgat gatataggcc aacaatacgg atggcccggc ccatttcagg gtactggcgg
     3661 cccccataaa caggccgacg ccaatagtgc cccccagggc gatgagttcg atatgtcgag
     3721 cttccagccc acgctgtagc tctggtttgt tatctgccat aaatcctcgt gttgtgtttg
     3781 catgctttcc ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg aatgttttcg
     3841 taattcagga gaaatggcaa ataaagcatt aaaaatttga atgctttgtg taataaaaaa
     3901 gcagacaggc gacggagtga ccactccgtc gctttacaaa gagaggaaaa tcagaggttg
     3961 ccggtgtagt gccagcgtaa ataacgcagc aaacgaagct gacgcttaat gcggctcggc
     4021 tgcgaaagca ggcggtagag ccactccagt cccagcgttt gccagatttt cggtgcgcgt
     4081 tttacgtgac cggtgaaaac atcgtaagtc ccgccaacgc ccatatacag cgcatctgga
     4141 tgcaccagac ggcagtcgcg catgaagatc tcctgctttg gcgatcccat cgcaacggtg
     4201 acgatttgcg ctccgctggc atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta
     4261 aaataaccat cctgactgcc aacgatattc acattccatt ggttgcgcag tttagcttca
     4321 gtttgcgcca gcacttcagg tttaccgccc acaagaaata ccggcgtccc ttctttgcct
     4381 gcgcgcgcca tcagctcttc ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc
     4441 gggtactttt tacgtactga acgtacaacg ctgatgccat ccgcatattt aaattcggca
     4501 gcgttaatta actccctgac ctcggcgtta tcttcaatgg tcagcatttt ttcagcatta
     4561 atggcaacca gcgttccctg cttaagctgc ccgtcagcaa acagataatc gagggcgtgc
     4621 tgcatatcac gccaaccaat caactgtaag ccacgcagcg tataggtcgg tgccgtggtg
     4681 ttgttattca ttgttatcct tcaacctgcg tccggagcga tgattttgta cgtttatgaa
     4741 tgagtccggc gctttcaaaa agccagtaca gcagttttgc gattatcaga catgcgccga
     4801 agaccacgat aaaaaagact acgcgtgaga caaacgaatc cagcccttca cgcgccagca
     4861 cgatcatatt gaagatcgcc ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat
     4921 taggctcgcg gttgcccagc tcatacagcc agtcgaacca tttgatgatc agaccaacca
     4981 cgatcgcccc gagcgggatg aacaacgcgc cgcccatcac caccagtgag cctataagcg
     5041 taggcgagat cgccagtccg gagtggttat tcagcacttc ccaggtaaag tagttggctg
     5101 agttcagcac catactcggg cgacccggcc acagccagga agggataaag acatagaaat
     5161 cgcggacaat tggagccagg ccctggaagt cgatgttgtc gtagttctgc aacagcaacg
     5221 ccagattctc ccacggggag aaggtgtcgc gagtgagata gagaaacgta tagaacgctt
     5281 catcgccgct cacattcatt ccatagcgtt ttagcgccat ccagaacatg ccaacaatcc
     5341 ccagcacgcc cgccgccgcc agcatccaca acgaaatcca gccgcgaata atgccaataa
     5401 acaggaagat agcgaatgcg atgatgatat tggcgcgagt gccgccgaca atcatataag
     5461 tcagcaagcc aaaggcgacc gtgctgacga ggaaaaacag ccacgctttg ctgtcctggc
     5521 gcagaaagta gaccaccagc atcgccggga tgaaaaagta aaagaagcgt tttaacgcca
     5581 cgccggagac ttcactggag aagatctgac tgtaggagtt aagccggaac agcaaaaagc
     5641 cgttgtgcat gaagaagatg ccgacgctta ccagcgcgat acccatcagg atcacccacg
     5701 taagattggt ctccacgcgg ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc
     5761 gtttgcgtag gcgggttttg taggtgacat agtaaaccgc gtagaagcag cccgcagaaa
     5821 gcaacgcctg caacaagatt tctggcggcg cgacaccaac atcaaagcga aataccagca
     5881 cgctggtcag cgggaagccg aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat
     5941 tgaagttaaa gcgcacacgg cgaaactcaa accaggtcag cgtggcaata aacagcgtgc
     6001 agagcagcca gaccacaaac aggccactga attgcagcag actcatgcga cctccccggc
     6061 ggcaatcgcc aacgcccgct gccagccttg tagatagtta gggctaaaga aggcaatggt
     6121 gtttttatcc accgacgcca actggcgctg cgcttcacgc acaatatcct cgttgagatc
     6181 gtcggtagta aacagcaccg gcaaatgctg ttccgtcata tcctgccaga acggattttc
     6241 ccggttaagc acacaaggaa tgcccgcctg aatcagtaag caaagcgtac caatgccctg
     6301 ctggcgggca aaaataaagt aaccaagatc gcactgacga agtagcgcca gataggcgtc
     6361 aaattccagt ttttcgctca aaatttgcag attttcttcg ctgaataact ccagccccgc
     6421 ctgacgaact tcctcaatgt acgcttcgtt attaggcgga tatcccatcg gcaccaccac
     6481 ttttaccgta tcgccaaatt gctgatgaac ggcgcgcaag gcagcaatat ggtcattgct
     6541 gcggtcaccg gagttcccca ccagaatggt cattttccct tcacgttgcc gatcattcgc
     6601 catcgtattg agagaagggt ccatccgcgt cgggaagtac agcagttcgc cccgcacctt
     6661 tggatgcgtt ttggcaaaaa agctcaaatc accgcgggtg gcaaatacac agccgactcg
     6721 cttttgcgcc aggcgacgta gcgggtaaaa aagcttatat ctcaagccac tggaaagctc
     6781 atacaggtct gccccccaga tatgccagaa aaactggctg ggcttaatgc caccactcag
     6841 cagagccagc cacagtgtgg gattgaactg accgtggaaa aaaaatcgct gctgacggtt
     6901 agcttttgct ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga aaaattgcac
     6961 agaaagcgcc ggacagctat cacttaagcc gtcgtccttg ccaacaacca taaactcgcg
     7021 cgcgtgctcg ctcgtcgcgg ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg
     7081 gtgagggata tccgatccca gtacgtgaat cagtacagtc atgcccgcct acgccagagt
     7141 aaaaacacgc cacaacaaag agaaaaatag acgatataag ttgccatata tgcctgcgcc
     7201 gcgcccagtg caccatgcgc agggattagc cagtgggcaa ataccatcaa taaagtgaac
     7261 tggctgactt ccgccagaat ataaaaccgc agtgacgctt tggcgatcac cagataacca
     7321 aagacataag cgcccacttt taacacatca cccactaact gccaggcaaa gagatcgcgc
     7381 atagcggtaa atttattcga caacagcaac cagatagcaa aatcacgcag caaccagacg
     7441 gtaaaactcg ccgccgccac tgccggtaag acgaatttca gcgatttaac cacttcccgg
     7501 gtgatatcgc gcttttccgt tagccgcgac aacgtgggca gcaaatatac gctgaacgat
     7561 gccgtaataa attgcaggta ggcatcggaa atactgctca ccccttgcca gatccccact
     7621 tcatcccagc tatactgcgc cgccagcagt ttacgcatca tgatgtaagc aacaggcaag
     7681 gtcaccgacg taatcaacgc catgagcgta aatttgctca actgccctgc cagaccgtta
     7741 tcccagctgg gtttcagata gcttaacggg atgacaccac gtttgatcaa catgatggcg
     7801 gcaggaatca ccaccagcgc gggaatcagc gccagaccca gcaacgcccc ttcataaccg
     7861 cccaaacggt aactgacgta gtacgcgaga acgccaatca agctgccgac aatcagcgat
     7921 aacgcattac ctgcggcatc gcgaaagcct ttcatcagcg ccagtaacag gttgccccag
     7981 gcgatcccca tttgcaccag cgccactaaa cgcaccagcc cctgatagtc ggtattacca
     8041 aacagtccct ggctgattgg cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag
     8101 aaaccaagta ccatcgctga tgaagtgccg accacgcggc gcagctgttg cggattatca
     8161 tggtactggg caacgtattt ggttacaccg ttaaagatgc cagctccggc aagcacgccg
     8221 agcacggtaa tcaactggcg gaaatttgcc gccagcccaa gccccgccgg accaaatgac
     8281 acagccagca acttaccgac cagtaacccg gcaccaatct tgaccagtgt actggccgcc
     8341 gtccacaagg acgcttttgc caacgacata tcaggagaag tagttcaaca aagtcgcaat
     8401 taccgtacgc tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc
     8461 gctctctttg gtggtgtagc gatcttcacc gtggaactca ccaaagcgtt cccccgcagg
     8521 gcaaccgtgc agcggaatgt aatggaacac cgccatgatt tccgcttctt tcagaaagtt
     8581 aatcaacgcg ctccggtcat caatatcccg cagtttaatg tagaacatat gcgcgttctg
     8641 cacgcagcca tcgggaatcg acggcagctc gatacgcccg gctttcgcca gaggcgctaa
     8701 cgcatcgtag tagttttgcc acagcgccag acgttgctgg ttgatacgat ccgctgcttc
     8761 cagttgcgcc cacaggtacg cagcttgcag atcggacatc aaatagctgg agccaatatc
     8821 gcgccaggta tatttatcga cctgaccacg gaagaactgg ctgcggttag tgcccttttc
     8881 acggatgatc tcggctcgtt cgattaacgc tttatcgtta atcagcgtcg cgccgccttc
     8941 accacccgcc gtatagtttt tggtttcatg gaagctaaag cagccaatat gaccaatggt
     9001 tcccagtgca cgccctttgt aagtggacat cacgccctga gcggcatctt ctaccacaaa
     9061 cagattatgc tttttcgcca acgccataat ggtgtccatt tcgcaggcca cacccgcgta
     9121 atggaccggc acgataacgc gcgttttgtc ggtgatcgcc gcttcaatca gcgtttcgtc
     9181 gatgttcatg gtgtccgggc gaacatccac aaaaacgatt tttgcgccac gcagcacaaa
     9241 ggcattggcg gtggagacaa aggtgtagct cggcatgatc acttcatcgc caggctggat
     9301 atcgagcagc agcgccgcca tctccagcga agcggtgcag gacggcgtca gtaacacttt
     9361 ggcgctgcca aaacgttgct ccagccactg ctggcagcga cgggtaaaac cgccatcgcc
     9421 acacagtttg ccgctaccca ttgccgactg catatagtcg agttcggttc ccaccaccgg
     9481 cggtgcgtta aatggaatca tgtgatcacc tgtataacca gtacgcggtg ctttctacat
     9541 tcgcaccact ttgtatgtat cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc
     9601 gcaaagttgt tttaccgcga gcatacgccc agtttagcgc cgtttgcatc agctcagcac
     9661 ctgcaccgcg tccagccagc aggccaattc gcgcatctgt cgcattgagt tcccgtaaag
     9721 agacataacc gcgaataccg ccggacgccg cacgtaaaat cagacattga tgatcaaagg
     9781 tgccgcgcac ggcattttca atccactgtg cataaaagcg tccgctggcg tcaggcgcat
     9841 accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg
     9901 gaatatcggt ctcttgtgcc actacagcac cactatcact gacattgttc acgggtagtg
     9961 ccaaatcaac ttcaccttct accagggaga atcccagctg ttgcagggca tccagttcac
    10021 ccgtatttga tgccgcaatt ttggcctgca cccgtgacca cggcgctaac acgtctggcg
    10081 tcaggagcgg tgcttcagac gtaatgcgca cgatggcgct gttaacacca aagaaggcgt
    10141 tttcccaggt tagtggctca atactggcgc ggacgggcac gaagtaactc cagcagatat
    10201 tggccgtagc cagttttcgc taatgaactg gcagcacgct tcacaccctc gtcatcgagc
    10261 cagccgttac gccaggcgat ctcttccagg caggcaatct taaagccctg gcgtttttct
    10321 actgtctgca caaaggtact ggcttcaatc aggctgtcgt gagtgccagt gtccagccag
    10381 gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg cctcgaggta catctggttg
    10441 atggaggtaa tctccagttc accacgctcc gacggcttca cctgctttgc gtactccacg
    10501 actttactgt cgtagaaata aagcccggtc accgcccagt ttgacttcgg ctgttttggc
    10561 ttttcttcca gcgagatagc gcggaaattg tcgtcaaact ccaccacgcc aaagcgttcc
    10621 gggtccatca cctgatagcc aaataccgtc gccccttccg tgcgcgccgc aacatgacgc
    10681 agcttcggac tgaagccctg accgaagaag atgttatcgc ccagcaccag acaagaaggt
    10741 tcaccgttga ggaaggtttc accgatgata aaggcctgcg ccagaccgtc cgggctgggc
    10801 tgttcggcgt attccagctg gataccgaac tcactaccat cgcccagcag gcgctggaaa
    10861 taacctttat cttccggcgt agtgatgatg agaatttcgc ggatatcggc caacatcagc
    10921 accgacagcg gatagtaaat cattggctta tcgtaaatcg gcaacagttg cttcgatacg
    10981 ccgcgcgtaa tcggatgcaa tcgggtgccg gaaccgcccg ccaggataat acctttcatg
    11041 tttgcctccg ctgaaaatta gcctttcaga cctaaacgct cgccctgata gctgccgtcc
    11101 tgcacctgct tccaccagct ttcattagcc agataccact gcaccgtttt acgcattcca
    11161 ctttcaaagg tttcctgtgg cagccagcca agttcacggg caattttcga agcatcaatg
    11221 gcataacgca gatcatgccc cggacggtca gcgacaaagg tgatcaagtc acgatagtgc
    11281 accacgccgt gcggcttgtt cggagccagt tcttccagca gctcgcaaat ggtttccaca
    11341 acatcgagat tcttacgctc gttgtgacca ccaatattat aggtttcacc gactttcccg
    11401 gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcca catacagcca gtcacggatt
    11461 tgctgcccgt tgccatatac cggcagcggt ttacccgcca gcgcgttgag gatcatcagc
    11521 gggatcagtt tttccggaaa gtggtaagga ccgtagttat tcgagcagtt ggtgataagc
    11581 gtcggcagac cgtaggtccg cagccaggcg cgcaccagat ggtcgctgct ggcttttgac
    11641 gcggaataag ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag
    11701 tgcaggtcac catatacttc gtcggtggag atatgatgaa aacggaacgc tgatttttta
    11761 tcttccgtca gcgtattcca gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca
    11821 atgttggttt caataaacgc tgccgggccg tcaatagaac ggtcaacatg gctttcggct
    11881 gccagatgca tgacacagtc tggctgatgc tcagtgaata cgcgtgccag ttctgcccga
    11941 tcgcagatat caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg tgccagcgac
    12001 atcaggtttc cggcgtaggt cagcttatcg accactacca ccgcgtcgct cgtttcgttg
    12061 atgatataac gcaccagcgc cgagccaata aacccggcac cacctgttat cagaattttt
    12121 ctcatcagcg ccagactcct ttggcatcga cgacatactg ctgatggaca ttgtcgccat
    12181 tgataacttt aaactgacta tgatcgacca gcatcaccag cacgtctgcc gttgccagcg
    12241 cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt tttcggcaac tggtggatgt
    12301 taggctcaac aaccagagtt tcgccgctat gccactgggc gatcagttca gcgatttcca
    12361 tcgccgggct ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca aagcaggcga
    12421 ttttcagttc actggcgcgt ttatcggtag ccgccaggca atcagccacc gccgctttca
    12481 cctgatcgat aacccagaac ggtttgtgat cgttcacttc gcgcgcggta cggataagcc
    12541 gcgcctgctg ggggttctgt gccacgataa accacggatc aacagcaatg cagtgaccgc
    12601 ccacgccagg gccaggctga agaatattaa cgcgagggtg acgattcgcc aggcgaatca
    12661 gttcccagac gttaatcccc tgatcggcac aaatcagcga caattcatta gcaaaagcga
    12721 tattcacatc gcggaagctg ttttcggtga gcttacacat ttccgccgtc cgcgagttag
    12781 tgacgacaca ctcaccttcg aggaaaattt tgtacagttc gctggcgcgg gtcgaacaaa
    12841 ccggcgtcat accaccaatc acgcgatcgt ttttaatcag ctcgaccata acctggcccg
    12901 gtaacacgcg ttccgggcag taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg
    12961 ggaaagtgag atccggacgc atctctgcta accattctgc catcttctcg gttgacccca
    13021 ccggcgaggt ggattcaagg atcaccagcg cgcctttttt cagcactggc gcaatggagc
    13081 gagcagccga ttcaacgtag gtcatatctg gctcatgatc gcctttaaac ggcgtgggta
    13141 cagcaatcag ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt
    13201 ctacggcagt ttttactacg ctcgccaaat caggctcgac gatatggatt tcgccacgat
    13261 tgatggtatc aaccgcgtgt tggttgatat cgacaccaat cacctgtttt tgccgtgagg
    13321 caaacgctgc tgctgtaggc agcccgatgt aacccagtcc gataacagaa atggtcgcaa
    13381 aactcatagt gatatccgat tattttttaa cgcttccaga atgcgagagc atgcctgacc
    13441 atcaccatac gggttatggg cgcggctcat agtttgatat tcgttttcgt cttttaaaag
    13501 acgcgtcact tcctcgacaa ttcgctgctt atccgtgcct accagacgca ccgtacccgc
    13561 cgtcaccgct tccggacgct cagtggtatc gcgcatcacc agcaccggtt tccccagcga
    13621 aggcgcttct tcctgaatgc cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag
    13681 ccagacaaac ggtaaatact cctggggatc gatcagaatg acatttttca catgccccag
    13741 aatgcgattg accggctctc tgacgttcgg gttgagatgc accggataga caatctggat
    13801 gtcctggtgc gtggtggcaa tgtctgccaa cgcgtggcag atttcttcaa agccacgacc
    13861 gaaactttca cgcctgtgac cggtcaccag aatcatcttt ttatcggggt cgataaacgg
    13921 gtaatttgcc gccagttctg aacgcagcgt atcgctgctc atcacctggt cacgcaccca
    13981 taacagtgca tcaataactg tattaccagt aatgaagatt cggctatccg caacgttttc
    14041 acgcagcaag ttttgccggg aagtttcggt tggagagaag tgatacatcg ccagatgccc
    14101 ggtcaatgta cggttagcct cttccggcca cggcgaataa agatcgcccg tgcgcagacc
    14161 agcctcaacg tgaccaacag gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt
    14221 cgtcgttgtg tcgccgtgaa ccagcacgac gtctggtttg aactcggcaa gaataggttt
    14281 tagcccttcc agaatccgac aggttatctc tgtcaggccc tgtcctggct gcattatgtt
    14341 gagatcgtag tcaggtacaa tggaaaagag tttcagcacc tgatcgagca tctcccgatg
    14401 ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct tttgccaacg catgcaccaa
    14461 cggcgccatc ttgatggctt ccgggcgcgt accaaataca gtcagtactt tcacatcgat
    14521 tctcttcgaa taagcggcga gcgcctttgc gctcaccgca gcagtgttgc tatttcgagc
    14581 aacggcgggt taatgcgaca ccagccccga tcagcccccc gacaatgccc cacataatca
    14641 tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc
    14701 gataggtctg aaaacgcgga tccagggttg gaccaacatt cagggtgttt aacatggccc
    14761 gattctgatc atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag
    14821 cctggagcat tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca
    14881 catctgtcgc actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgttcaatac
    14941 tgttcatccg gcggtcgtag atggctttcg ccacctcttc ctgacgcttc acctgtgctt
    15001 tcatctggat ggtacgcgcc gcccatgcgc ctttcagctc atcattcaga tggctggctg
    15061 cacgctggct ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt
    15121 cagcaataag cttcacgctg tcattgaccg cgcgggtaaa gtctccgggg ataaactgga
    15181 tgttgttaat catttcatcc agcaacgccg catcggcttt gctgttgccc accatccgct
    15241 gtttgtaata gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca
    15301 taacaaactc tttataggct tcgtccatga ccgatggttg gtcggcagaa gccatgtttg
    15361 aacggacatc caggttacgc aaaaattgct gctgcgagta atatcccccc agcatattca
    15421 ccgttggacg atcggtaatc gccgtcgagc tccactcctg acgagcaaaa aaagtatacg
    15481 ccagcgcgat taacgcaaat gccagcccca tgccaataat ccatagcttc ccagcccaca
    15541 aggtacgaaa caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag
    15601 gcattggttg tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt
    15661 tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttaat
    15721 gcaatatcca tagaggaaga atgctagcaa aaagagcacc agcatgaccc actccgggac
    15781 aaaatgagaa tattctgcca gcacgccaat ggaagcgagc agtgccgcag caagggtaat
    15841 cagcacaaac gcctgacggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg
    15901 acggtcagga gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat
    15961 atccattagc ggaatggcga ttatccacaa agcggtaacc gggctgatgg gatgggtttt
    16021 gccctgggtc gtttcgagca ggatccagat aacggtaaaa ccaatcagcg tactgcccgc
    16081 atcacccata aagactttgt agcggcgacc caggatacca aggttaagca tgatgtatgg
    16141 caggatggcg gcgatcatcg caaagcacca gattgcgagg ctggtttgcc cgtcgaacca
    16201 caaaatcata ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat
    16261 gccatcaacc atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta
    16321 accaaacggt ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata
    16381 aagtttgccg aacaccatca taacaatgcc aacagcggcc tgtatggtgg cacggatttt
    16441 tacgctgata tcaaaacggt catccagcgc gccaatgaaa acaagcacac cggcacaagc
    16501 gagatagaga gatgcatgcg gaatatagta atcgacaatt ccgaacgtga agcaaatccc
    16561 tgcgtaaacc gaaatccccc caacgagagg tatcaatccc tggtgacgtt tgcggaagtt
    16621 tggtttatcc actaaaccga ctttttttgc caccttacgg gcaaaaaaca gaaacagtgt
    16681 cgtgaataaa aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc
    16741 tctcagagaa aattattagc agaagtataa ccacgaagac ctttattcag aagggaaagt
    16801 ccgttccgaa cccaccagtc ctgtgcaaaa attaaccatt tgatatgcaa attgcataac
    16861 acgccagcaa attccaggaa ttttcctaac atcgccagcg cggcattaag attacagata
    16921 taaaagcaaa a
//
LOCUS       DAZPJP010000076        16909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPJP010000076 DAZPJP010000000
VERSION     DAZPJP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.076"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHU9J_002299"
     CDS             92..1132
                     /locus_tag="ACHU9J_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1905817.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHU9J_002300"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHU9J_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1905818.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHU9J_002301"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHU9J_002301"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1905819.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHU9J_002302"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHU9J_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1905820.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHU9J_002303"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHU9J_002303"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1905821.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHU9J_002304"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHU9J_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1905822.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHU9J_002305"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHU9J_002305"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1905823.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHU9J_002306"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHU9J_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1905824.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHU9J_002307"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHU9J_002307"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1905825.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHU9J_002308"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHU9J_002308"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1905826.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="ACHU9J_002309"
     CDS             complement(12944..14125)
                     /locus_tag="ACHU9J_002309"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1905827.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="ACHU9J_002310"
     CDS             14380..15609
                     /locus_tag="ACHU9J_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1905828.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="ACHU9J_002311"
     CDS             16069..16701
                     /locus_tag="ACHU9J_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1905829.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4463 a         4436 c         3912 g         4098 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ccctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac gtctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgc
//
LOCUS       DAZPJP010000077        16893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPJP010000077 DAZPJP010000000
VERSION     DAZPJP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.077"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /locus_tag="ACHU9J_002312"
     CDS             complement(<1..262)
                     /locus_tag="ACHU9J_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1905830.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="ACHU9J_002313"
     CDS             369..665
                     /locus_tag="ACHU9J_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1905831.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHU9J_002314"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHU9J_002314"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1905832.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHU9J_002315"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHU9J_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1905833.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHU9J_002316"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHU9J_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1905834.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="ACHU9J_002317"
     CDS             complement(3472..3645)
                     /locus_tag="ACHU9J_002317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905835.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHU9J_002318"
     CDS             3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHU9J_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1905836.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHU9J_002319"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHU9J_002319"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1905837.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHU9J_002320"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHU9J_002320"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1905838.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="ACHU9J_002321"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="ACHU9J_002321"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1905839.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHU9J_002322"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHU9J_002322"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1905840.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHU9J_002323"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHU9J_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1905841.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10765..10818
                     /locus_tag="ACHU9J_002324"
     CDS             10765..10818
                     /locus_tag="ACHU9J_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905842.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHU9J_002325"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHU9J_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1905843.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="ACHU9J_002326"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="ACHU9J_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1905844.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHU9J_002327"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHU9J_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1905845.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="ACHU9J_002328"
     CDS             14052..15254
                     /locus_tag="ACHU9J_002328"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1905846.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16622)
                     /locus_tag="ACHU9J_002329"
     CDS             complement(15570..16622)
                     /locus_tag="ACHU9J_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR1905847.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4331 a         4284 c         4384 g         3894 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct caagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgcgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acctaacgcg
    16561 atgcaaatta atcccataat caaagggatt aatttgaaaa gccacccgat atatatgttc
    16621 actttaaact cctcaggtat ccctgctaat aatgaaatgt gcattaatga taatttcagc
    16681 ctgttaatat aacgacgaga tatcattaaa gacaaatgca tagcataact atagcggggt
    16741 ttactgtgcc gagtgccatt ttttaccggt taaaaggcca tgataaataa atatttatcc
    16801 taagattaat tcttattctg ttgttttctt ttacaattat cttccaggga tggattgttt
    16861 tccattattt agtaaaagac cggatagtct cgg
//
LOCUS       DAZPJP010000078        16678 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPJP010000078 DAZPJP010000000
VERSION     DAZPJP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.078"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            81..1220
                     /gene="arnB"
                     /locus_tag="ACHU9J_002330"
     CDS             81..1220
                     /gene="arnB"
                     /locus_tag="ACHU9J_002330"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1905848.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1224..2192
                     /gene="arnC"
                     /locus_tag="ACHU9J_002331"
     CDS             1224..2192
                     /gene="arnC"
                     /locus_tag="ACHU9J_002331"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1905849.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2192..4174
                     /gene="arnA"
                     /locus_tag="ACHU9J_002332"
     CDS             2192..4174
                     /gene="arnA"
                     /locus_tag="ACHU9J_002332"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1905850.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4171..5061
                     /gene="arnD"
                     /locus_tag="ACHU9J_002333"
     CDS             4171..5061
                     /gene="arnD"
                     /locus_tag="ACHU9J_002333"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1905851.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5061..6713
                     /gene="arnT"
                     /locus_tag="ACHU9J_002334"
     CDS             5061..6713
                     /gene="arnT"
                     /locus_tag="ACHU9J_002334"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1905852.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6710..7045
                     /gene="arnE"
                     /locus_tag="ACHU9J_002335"
     CDS             6710..7045
                     /gene="arnE"
                     /locus_tag="ACHU9J_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1905853.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7045..7431
                     /gene="arnF"
                     /locus_tag="ACHU9J_002336"
     CDS             7045..7431
                     /gene="arnF"
                     /locus_tag="ACHU9J_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1905854.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7425..7691)
                     /gene="pmrD"
                     /locus_tag="ACHU9J_002337"
     CDS             complement(7425..7691)
                     /gene="pmrD"
                     /locus_tag="ACHU9J_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1905855.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7801..9156)
                     /gene="menE"
                     /locus_tag="ACHU9J_002338"
     CDS             complement(7801..9156)
                     /gene="menE"
                     /locus_tag="ACHU9J_002338"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1905856.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9153..10115)
                     /gene="menC"
                     /locus_tag="ACHU9J_002339"
     CDS             complement(9153..10115)
                     /gene="menC"
                     /locus_tag="ACHU9J_002339"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1905857.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10115..10972)
                     /gene="menB"
                     /locus_tag="ACHU9J_002340"
     CDS             complement(10115..10972)
                     /gene="menB"
                     /locus_tag="ACHU9J_002340"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1905858.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10987..11745)
                     /gene="menH"
                     /locus_tag="ACHU9J_002341"
     CDS             complement(10987..11745)
                     /gene="menH"
                     /locus_tag="ACHU9J_002341"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1905859.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11742..13412)
                     /gene="menD"
                     /locus_tag="ACHU9J_002342"
     CDS             complement(11742..13412)
                     /gene="menD"
                     /locus_tag="ACHU9J_002342"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1905860.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13501..14796)
                     /gene="menF"
                     /locus_tag="ACHU9J_002343"
     CDS             complement(13501..14796)
                     /gene="menF"
                     /locus_tag="ACHU9J_002343"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1905861.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14875..15180)
                     /gene="elaB"
                     /locus_tag="ACHU9J_002344"
     CDS             complement(14875..15180)
                     /gene="elaB"
                     /locus_tag="ACHU9J_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1905862.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15235..15696)
                     /locus_tag="ACHU9J_002345"
     CDS             complement(15235..15696)
                     /locus_tag="ACHU9J_002345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1905863.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15761..16678
                     /gene="rbn"
                     /locus_tag="ACHU9J_002346"
     CDS             15761..16678
                     /gene="rbn"
                     /locus_tag="ACHU9J_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1905864.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLSKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3848 a         4543 c         4285 g         4002 t
ORIGIN      
        1 cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt
       61 caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg
      121 tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga
      181 agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg
      241 tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg
      301 atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgttgg
      361 gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga
      421 tcgagtcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc
      481 cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag
      541 atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg
      601 ctattttttc atttcatgcc attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa
      661 ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg
      721 atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct
      781 ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta gcaaaattag
      841 agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc
      901 tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta
      961 ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa
     1021 aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc
     1081 gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt
     1141 ctttgccgct gttcccggat atgactaccg ccgatgccga ccgtgtcatc acagcccttc
     1201 agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg
     1261 ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag
     1321 cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata
     1381 attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt
     1441 tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt cacgttactg
     1501 gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc
     1561 tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg
     1621 acagctggtt tcgtaaaaca gcttcgaaga tgattaacag gcttattcag cgcaccaccg
     1681 gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg
     1741 cgatgttgca ctgccatgaa cgtagcacct ttatcccgat tctggcgaat atcttcgccc
     1801 gccgtgccat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa tccaaataca
     1861 gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc
     1921 tacgtatgct gagtctgctc ggcagcatta ttgcgattgg aggttttagc attgcggtgc
     1981 tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc
     2041 tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga ttgctcggtg
     2101 aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag
     2161 ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga
     2221 tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt
     2281 tacccatacc gataatcccg ctgaaaaagc cttttatggt tcggtggctc gtctggcggc
     2341 ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt gggtggaacg
     2401 cattgcccaa ctctcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga
     2461 cgagattttg cagctcgctc ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa
     2521 atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt
     2581 tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc aactgcgcgt
     2641 tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg
     2701 ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg aaattgccca
     2761 gcgcgaaaac gaagccacct gttttggtca cagaacgcca gatgacagtt tccttgaatg
     2821 gcataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg
     2881 tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca
     2941 tgccagcaaa gcacagccgg ggagcgtgat ttctgttgcg ccactgctga ttgcctgtgg
     3001 cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc
     3061 gcaattagcg cagatgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg
     3121 caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca
     3181 tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag
     3241 cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag gcgatatcag
     3301 tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct tgccgctggt
     3361 ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt
     3421 tgaagagaat ctgcgcatta tccgctactg cgttaagtac cgtaagcgaa tcatcttccc
     3481 gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa
     3541 tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcaa aacaattact
     3601 tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc
     3661 gtttaactgg atgggaccac gactggataa ccttaatgca gcgcgaattg gcagctcccg
     3721 cgctattacg caactcattc tcaatctggt agaaggttca ccaattaagc taattgatgg
     3781 cggaaaacaa aaacgctgct ttactgatat ccgggacggt atcgaggcgt tgtaccgcat
     3841 tatcgaaaat gcgggaaatc gctgcgacgg cgaaattatc aacattggca atcctgagaa
     3901 cgaagcgagc attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct
     3961 gcgccatcac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca gctactacgg
     4021 caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc gccgctgcct
     4081 ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct
     4141 gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg cattgatgtc
     4201 gatacctttc gtggcacccg tgaaggcgtg ccacgtctgc tggaaatctt gagtaagcat
     4261 aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg
     4321 cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc atcgctttat
     4381 ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat
     4441 gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac
     4501 catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga cgatattgca
     4561 cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg
     4621 ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac
     4681 agcgattgtc gtggggccat gccgttccgt ccactgctcg aatcaggaaa ccctggcact
     4741 gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca
     4801 gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat
     4861 accattcatg cagaagttga aggctgcgct tatcagcata attttgtgga tctcctcaaa
     4921 cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg
     4981 ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa
     5041 caaattgcgg gtagtcgctg atgaaatcgg tacgttacct tatcggcctc ttcgcgttta
     5101 ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc
     5161 gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg ccccatctgt
     5221 tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat
     5281 ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa
     5341 ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac
     5401 tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc
     5461 tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc
     5521 aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca
     5581 tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat
     5641 gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca
     5701 gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag cctgacttct
     5761 ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata
     5821 gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat
     5881 tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt
     5941 tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggt aaattgccca
     6001 cctatattct ttcctgcttt gcatctctgg caatgctgat ggcgcattac gctttgctgg
     6061 cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggct
     6121 tcactggcat tattgccaca tttgtggtct ccccgtgggg accaatgaac acgccggtgt
     6181 ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat
     6241 ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtcttttgcc gcgctttgcc
     6301 cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac
     6361 atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta
     6421 ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca
     6481 tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat
     6541 ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta
     6601 cactcgtgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag
     6661 atgccatcga tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct
     6721 aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac
     6781 ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct
     6841 ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg
     6901 cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact
     6961 gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta ttggcggcat
     7021 tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag cgtcattatt
     7081 gcctcagttg cgcaattaag cctgggtttt gcggcgagtc atctgccgcc gatgacgcac
     7141 ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc
     7201 gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg
     7261 ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg
     7321 gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt
     7381 atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg agttttccct
     7441 gccactttac actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc
     7501 accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg cgagagtaaa
     7561 tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca
     7621 gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgacc
     7681 agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca
     7741 gataaaccta ttatcaacgg caatggaatg gcaaaattgc gaagtgaaac taatgatttc
     7801 ttaatcctgg cgttgcaccc actcttttaa cgcctgacgt gaaattttaa taccgccgtt
     7861 tttcagctcc ggcggtaaag ttagccagcg caccggttgt tgaaaacgag ccagcttatc
     7921 tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggtcg
     7981 atgaccaaac tccttgtcgg caacggggac gataaacacc tgcaaaaccg caggatgtgc
     8041 agcaattacg cgctcgactt cttccggctg aataccctct ccgccactga agaacagatt
     8101 gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc
     8161 gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc
     8221 catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag
     8281 cggcgaacca acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg aggcaaactc
     8341 ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc
     8401 gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac
     8461 cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg ccagcatttg
     8521 ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat
     8581 aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc
     8641 aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat aagtatggac
     8701 agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg
     8761 ctgccacgta gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg ttaatgccgg
     8821 aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg gcagcaattc
     8881 ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc cgcattgcag
     8941 taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct
     9001 gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc gagcacaaag
     9061 ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg
     9121 ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctccag tacatcagcg
     9181 tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg catcagatcc
     9241 agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg cgccagttgc
     9301 gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc cagcgcatgc
     9361 gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg tttgataact
     9421 accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag gctttcatcc
     9481 caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac
     9541 ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc
     9601 tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt
     9661 gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg
     9721 accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg
     9781 ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc
     9841 gccaatgcac agcttacgcc aaaggcaacg gaaggcatct gcggtagctc gccatcgcct
     9901 gccagccagt tatttaccca agcaagcagc acaatttgcg cctcttccca ggtttcctga
     9961 ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccttc acgcaggcaa
    10021 acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccatccc cgcgtccatg
    10081 gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga
    10141 aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt
    10201 agaacagcat ggtggcgttg cccgccagct cctgcagacc cgcctgaccg tcacagtcgg
    10261 cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc
    10321 accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca
    10381 tatccagcgc ctgttttgca tcgtactgac ggcacaggaa ccagatttca cgcgcttttt
    10441 tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt
    10501 tcgggccagt ctgaccgaag atggcattat ctgccgcgat agtcaggtcg cacatcatgt
    10561 gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac
    10621 aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg gaatcatctt
    10681 tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc
    10741 ctgcgccagt cagaatgatc acgccgacgt tgtcgtcata acgcgcatct gccagtgcct
    10801 ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg
    10861 tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa ccttcggagc
    10921 agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc
    10981 ctttagtcag aaacgcaaga tctgcgccag acttgcgata acgccagcgg gattttcccg
    11041 atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccaa
    11101 ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct
    11161 aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag
    11221 ggttgcgcca ttattgttac tgcgcagcgc caccagctcc cggcgttgat cgtcattgag
    11281 cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgcg
    11341 aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt cagcattttg
    11401 caacccagga tgcccgcctt cgacaacaac cccacaaagc cccggcatcc cctggcaagc
    11461 cgccatcatc gccacccgtc ccccaagcga gtaccccacc aaccagaagt taaggatgtt
    11521 gtaactaacc agggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat
    11581 cgccgccgaa ccaccgtgac ctgggagata aacatacaac cgtgagtagt cggcaaacgc
    11641 ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
    11701 gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
    11761 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaattacc
    11821 gtggtggttg gcgtgcgcca ggcatcggca agtgccgttt caagttcctg ccagttttgc
    11881 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
    11941 atcagataga aacgctcgcg ctcgcttttc ggcgttggca gcagcgagaa aatttgcccg
    12001 ccgttgttgt tcaccacaat taatatcagc ggcgcggaaa cctgacgcaa taacgccagc
    12061 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
    12121 gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc cccacggttg
    12181 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg
    12241 ctgttaccga caaacaattg cccctgttca ggcagatagt cgctgatgcg atgcgccagt
    12301 tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg ttccgccagg
    12361 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
    12421 gcaatattgg caattaagcg gcgtccacga tggtgcgccg gatcaagccg cccttcgatg
    12481 tcatccacaa tccagtactc ttcgggttca cagcttgcct gccattgcag gagccgtttg
    12541 cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gttgcagttc gctggtcgct
    12601 ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg cgacagcacg
    12661 tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt gccctcttcc
    12721 gcactcatgc gcccggcaac caccacgccg cgtttttgtc gccagaagaa ccagtcgcgc
    12781 tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
    12841 tcgcccagcc gctgttgcca gctaagcccg gtgtcgtcca tttcgccata cagcggttcg
    12901 gcaaacgggc agttgatatg gactccacca gaatgaagcg taccgagagc atggtcgatg
    12961 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc
    13021 gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc gcagtcaatc
    13081 agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa cccagcttca
    13141 atcagtgccg gatagagatt tgctaccgcc gtaccggagg tcacaatcac cgccaccggc
    13201 tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc atcgaaatgg
    13261 gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
    13321 ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
    13381 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
    13441 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
    13501 ctattccatc tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc
    13561 ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc
    13621 gctaattttt gctgagcgca gggaaacgca gaattcgctt tgctgtaatg agagatagcc
    13681 cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc
    13741 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
    13801 attatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag
    13861 acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta accgttggca
    13921 gatatcttcc accaccagca tattctcgcg ctggttttta tcatccgcca tcagccactc
    13981 acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg ccagcgcttc
    14041 agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc
    14101 attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc
    14161 agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag
    14221 caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt
    14281 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa
    14341 gggcttgata ctcaccagtg tggcgataaa ttctcttgcc ttaatcgcat cgtgctgaag
    14401 ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc
    14461 caggcgtggt aaaagtaaat tgccctgcga cgggtcaaac gcattcagcc cccaaatgcg
    14521 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
    14581 acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
    14641 ttgcgggtat gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
    14701 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
    14761 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
    14821 tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
    14881 gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca
    14941 gggtttttcg tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata
    15001 actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
    15061 tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca cctcttccag
    15121 tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat
    15181 tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc
    15241 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt
    15301 cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta
    15361 aacaggctta tcaggccagt gatgcgtgca ggtgtcgagg gttttactca tcagttgctg
    15421 ccccactttt tcgccacgca acgcttcact gacaatcacc cgaccaataa cgaccggctc
    15481 aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat ttttccaacc
    15541 gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt
    15601 ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga
    15661 taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc
    15721 cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg
    15781 tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca
    15841 acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct
    15901 actgcatacc gcctttaacc ctgggaaact ggacaagatt tttatcagtc accttcatgg
    15961 cgatcatctc tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
    16021 acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat
    16081 tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct
    16141 cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg aatgttatgg
    16201 ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc
    16261 tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga
    16321 agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct
    16381 cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctgtcta aaggtgtcga
    16441 tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg
    16501 ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg gcaagctaat
    16561 cattacccac gtcagctcgc gctatgatga caaaggttgt caacacctgt tacgtgaatg
    16621 caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta acgtttaa
//
LOCUS       DAZPJP010000079        16323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPJP010000079 DAZPJP010000000
VERSION     DAZPJP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.079"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..80
                     /locus_tag="ACHU9J_002347"
     CDS             <1..80
                     /locus_tag="ACHU9J_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905865.1"
                     /translation="KNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(154..1356)
                     /gene="ubiI"
                     /locus_tag="ACHU9J_002348"
                     /gene_synonym="visC"
     CDS             complement(154..1356)
                     /gene="ubiI"
                     /locus_tag="ACHU9J_002348"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1905866.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1379..2557)
                     /gene="ubiH"
                     /locus_tag="ACHU9J_002349"
                     /gene_synonym="visB"
     CDS             complement(1379..2557)
                     /gene="ubiH"
                     /locus_tag="ACHU9J_002349"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1905867.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2554..3879)
                     /gene="pepP"
                     /locus_tag="ACHU9J_002350"
     CDS             complement(2554..3879)
                     /gene="pepP"
                     /locus_tag="ACHU9J_002350"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1905868.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3905..4483)
                     /gene="ygfB"
                     /locus_tag="ACHU9J_002351"
     CDS             complement(3905..4483)
                     /gene="ygfB"
                     /locus_tag="ACHU9J_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1905869.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4651..4980
                     /gene="zapA"
                     /locus_tag="ACHU9J_002352"
     CDS             4651..4980
                     /gene="zapA"
                     /locus_tag="ACHU9J_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1905870.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5022..5205
                     /gene="ssrS"
                     /locus_tag="ACHU9J_002353"
     ncRNA           5022..5205
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHU9J_002353"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5226..5828
                     /locus_tag="ACHU9J_002354"
     CDS             5226..5828
                     /locus_tag="ACHU9J_002354"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1905871.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5929..5988)
                     /locus_tag="ACHU9J_002355"
     CDS             complement(5929..5988)
                     /locus_tag="ACHU9J_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1905872.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6320..6379)
                     /gene="ibsC"
                     /locus_tag="ACHU9J_002356"
     CDS             complement(6320..6379)
                     /gene="ibsC"
                     /locus_tag="ACHU9J_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1905873.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6608..7840)
                     /gene="serA"
                     /locus_tag="ACHU9J_002357"
     CDS             complement(6608..7840)
                     /gene="serA"
                     /locus_tag="ACHU9J_002357"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1905874.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8096..8755)
                     /gene="rpiA"
                     /locus_tag="ACHU9J_002358"
     CDS             complement(8096..8755)
                     /gene="rpiA"
                     /locus_tag="ACHU9J_002358"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1905875.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9185..10078
                     /gene="argP"
                     /locus_tag="ACHU9J_002359"
     CDS             9185..10078
                     /gene="argP"
                     /locus_tag="ACHU9J_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1905876.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10281..12425
                     /gene="scpA"
                     /locus_tag="ACHU9J_002360"
     CDS             10281..12425
                     /gene="scpA"
                     /locus_tag="ACHU9J_002360"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1905877.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAYDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12418..13413
                     /gene="meaB"
                     /locus_tag="ACHU9J_002361"
     CDS             12418..13413
                     /gene="meaB"
                     /locus_tag="ACHU9J_002361"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1905878.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13424..14209
                     /gene="scpB"
                     /locus_tag="ACHU9J_002362"
     CDS             13424..14209
                     /gene="scpB"
                     /locus_tag="ACHU9J_002362"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1905879.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14233..15711
                     /gene="scpC"
                     /locus_tag="ACHU9J_002363"
     CDS             14233..15711
                     /gene="scpC"
                     /locus_tag="ACHU9J_002363"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1905880.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15708..>16323)
                     /locus_tag="ACHU9J_002364"
     CDS             complement(15708..>16323)
                     /locus_tag="ACHU9J_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1905881.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3984 a         4389 c         4148 g         3802 t
ORIGIN      
        1 caaaaaacac taaatcaaaa aataatgaca ttagaaaata taatgcgaaa acggaggtga
       61 aattagttta tttcaaatga ggaaaatctc ccggcgaaaa aaccgggaga tgaaagtgtg
      121 atgggtatca aataaacagc agaagagaaa tttttaacgc agccattcag gcaaatcgtt
      181 taatcccatt gcctggcgga taagttgtgg tttaacgcca ggaagcgtgt cggccagttt
      241 caaaccaata tcacgcagca gttttttcgc cggattggta ccggaaaaca gatcgcggaa
      301 tccctgcata ccagccagca tcagcgccgc actgtgcttg cggctacgct cataccggcg
      361 caggtaaatg tactgcccga tgtctttccc ctgacgatgc aaccgtttca gttcagcaat
      421 cagctctgca gcatccataa agccaagatt tactccctgc cccgccagcg ggtgaatggt
      481 atgcgcggcg tcgcccacca gcaccagacg gtgcgcggca aactggcgcg cataacgccc
      541 cgtcagtggg aacacctgac gcgcgctctc aaccttgcat aagcccaggc gattatcaaa
      601 agcgatattt aacgcgcgat taaattcatc ttcacttgcc tgctgcatcc gctgcgcttc
      661 ctctggcgac agtgaccaga caattgagca aagatgcgga tcgctaagcg gtaaaaaggc
      721 cagaatgcct tcgccatgga aaacctgccg cgccaccgca tcatgcggtt cttccgtgcg
      781 aatggtcgct accagcgcgt gatgctgata atcccagaaa gtcagcggaa tatctgcttt
      841 gttgcgcaac caggaattag caccgtccgc gccaatcacc agacgcgccg ttaacatgct
      901 gccatctttc agcgtcagga aagtttcatt ttctccccag gcgacctgct gtaattctgc
      961 gggggccaac agagtgatat ctgacgactg ctgcgctttg ttccacagcg cgtagtgaat
     1021 cactgagttt tcaacgatat gcccaagatg gctatagccc atgctttgat cgtcaaacga
     1081 aatgtgacca aaactgtctt tgtcccacac ttccataccg tgataacaac tggccctacg
     1141 agagagaatg tcctgccaga cgccaagacg ggtgagtaat ttttcgctgg cggcattgat
     1201 agccgaaacg cgcagttgtg gtggtgcatc cgccgccaga ggttccggta cgcgctgctc
     1261 cagtacggca acgcgtaagc cgctcccctg taagccgcag gcaaccgcca gccccaccat
     1321 gccgccacca acaatggcta catcaacact ttgcattgtt tattccttaa aaccgccttc
     1381 aacgcgccac ccaaccgagg gtgcgctgcg ccagcacatc gcgtgccggg gtgaataatt
     1441 ccatcgtcat cagtccgatg ttgcgcccga caaccagcgg tgcccaacgg ttggcaaaaa
     1501 gatgtaccag gctgtccgtg acgccgatgg ttgcttcgcg atcgctctgt cgacgctgct
     1561 gatagcggca caatatgccg taatcaccga tgtcttctcc gcgctcatgc gcctgagtca
     1621 gggtttccgc aaggctcatg acatctcgca taccgaggtt aaacccttgc ccggcaatcg
     1681 ggtgcagagt ttgcgccgca ttgcccacca gcacggtacg atgggtaata gctctggcgg
     1741 cgcgggttaa cgccagcgga taagcactgc gtttaccggc gtgggtaatt tttccaagtc
     1801 gccagccaaa ggccgactgg agttcacggc aaaacttctc gtcgctccac gacagcacct
     1861 cttcgcgccg ttccagtgga tgacaccaga ccagcgaaca gcgtccgtca gacatcggca
     1921 acatcgccag cgggccatgt tgcgtgaagc gttcaaaagc gcggccttcg tgcgcaacgg
     1981 aagtcgcaac gttggcaatc acggccagtt gttcgtaagg ctcctgctgc cagtcaacgc
     2041 cgcacgcggt ggctaacgct gaatgggtgc catcagctgc taccagcacg cggcccgtca
     2101 gcgtctcgcc actctccagc gtcacttcaa cgtgactctg agtacgggca acgttagcca
     2161 cgcgatcagg gcaatgcagc gttacgccag gtgctttacg cagcaatgca aacagccgtt
     2221 gcccgacatt gtgcaattcg acaacctgtc ccagcgccgc cagttggtaa tcttctgcgg
     2281 cgagggtgac aaatccagcg tgaccacgat cgctgacatg cacggtagtg atggcggttg
     2341 cgtaatccgc cagagattgc cagacgccga tgcgcgccag ttgctgacag gtccccgcag
     2401 ccagcgcaat agctcgtcca tcaaagcccg gatgagcatg tgactctggc gcggtcgctt
     2461 caatcaaatg taccggcagc gccccgtgac ttaaccggga aatagccagc gccagcgtcg
     2521 cgcccgccat gccgccaccg acgatgatta cgctcattgc tttctcgcag ctgccatcaa
     2581 cgcttcgatt tcttccggct ttttcaccac gctggcggtg aggttttcgt tgccggtttc
     2641 ggtaatcaca atgtcgtctt caatacgaat gccgataccg cgatattgtt ctggcacatc
     2701 cgcatccggc gcgatataca gaccaggctc tacggtcagt accatccccg gttccagaat
     2761 gcgcgagcga tcctgaccat aaacgccaac gtcatggaca tccagtccta accagtggct
     2821 aaggccatgc ataaagaaag gacgatgggc gttctgagcg atcagttcat caacatcacc
     2881 tttcaggatg ccgagtttta ccaggccgct aaccatgatg cgcaccactt caccagtgac
     2941 ttccagaatg gaagttcccg gacgatacag gcgcaggctg gtttcgagag actccagcac
     3001 aatgtcgtag atttcacgct gggcctgggt gaatttgccg ttgaccggga aggtgcgggt
     3061 aatatcgccc gcgtaacctt tgtattcaca acccgcgtca atcaacacca ggtcaccgtc
     3121 gcgcagttca ctctcgtttt cggtgtagtg cagaatgcag ccgttttcac cgctgccgac
     3181 aatggtgtta taggacggat agcgcgcacc gtggcggtta aattcgtggt gaatttcgcc
     3241 ttccagatgg tactcgaaca ttcccggacg gcatttttcc atcgcccgtg tatgtgccat
     3301 ggcggtgatt tctcccgcgc ggcggagtac ggcaatctct tctggcgatt tgaacaggcg
     3361 catttcatga acaacaggac gccagtcgat catcgttgcc ggtgcggtga gattttgccg
     3421 cgaaccttta cgcagttttt ccagcgcact gttcatgatt tcatcagcat atgcatattc
     3481 gccctgggca tggtaaacca catccaggcc gttaagtagt tgataaagtt gctgattgat
     3541 ttcgctgaat gccagtgcgc ggtcaacgcc cagtttctct ggcgcggcat cctggcctaa
     3601 gcgacggcca aaccagatct ccgccgtcag gtcgcgaacg cggttaaaca gaacgctgtg
     3661 gttatgagtg tcatcgcttt taatcagcac cagcaccgct tccgattcgt taaagccggt
     3721 gaagtaccag aagtcactgt tctgacgata ggggtattcg ctgtcggcgc tacgtgttac
     3781 ttctggtgca gcaaaaatca gcgcggcgct gccgggttgc atttgctcca ccagggcctg
     3841 acggcgacgc tgaaactctt gccgggatat ctcactcata acactctcct tacgtttttt
     3901 gtttttagtg tagagtcggt ttttgtactt ctggcgcggt cggttgcgga tgagtaaagg
     3961 tgtcgtggca taacagcgcg gcaacacgaa cgtattcgat gatctcttca agcgacattt
     4021 caagctcttc ctgatcttcg tcttcgtcgt aacccagttg cgcaatgtta cgcagatcgt
     4081 cgatagcttc accggtttcg ccggtcactt tatccagctt cggttgcgta acgccaagac
     4141 caagcaggaa gtgattgacc caacctgcca atgcatcagc ccgatcgaaa acgctgacat
     4201 catcgccatc aggcagataa agctgaaaaa ggaagccgtc atcctgcagg gcatcgctgg
     4261 tggcagagtg cattttacgc agtgcctgtg ccagctcatg accgaaagcc atgccttcgt
     4321 tcgtcaggtc gtgaagtagc ggcagccatg agctgtcatc gttaccgcca catatcatcc
     4381 cactgattaa accatgcatc tcggcagggg tcagccccgt cccttgttgg ttcagatact
     4441 ggttcatttc gttgtaacca ggcatttcgt tctgtataga cataagcatt cgtcgtcaaa
     4501 gggaggaata ttcatgatat gctaccactt tgggccctgg tggaccagaa aagggcttgt
     4561 ctcttctcat cagggtagct atagtgtcgc cccttcgcag accatgggtc taaagacgaa
     4621 ggcagcgcag tcaatcagca ggaaggtggc atgtctgcac aacccgtcga tatccaaatt
     4681 tttggccgtt cactgcgtgt gaactgcccg cctgaccaaa gggatgcgtt gaatcaggca
     4741 gcggacgatc tgaaccaacg gttgcaagat ctgaaagaac gcactagagt cacaaatact
     4801 gaacagttgg tcttcattgc cgcattgaat atcagctatg agttagcgca agaaaaagcg
     4861 aagactcgtg actacgcggc aagtatggaa cagcgtattc ggatgctgca gcagaccata
     4921 gaacaagcgt tacttgaaca aggtcgcatc accgaaaaaa ctaaccaaaa ctttgaatga
     4981 cacttttcgg tttactgtgg tagagtaacc gtgaagacaa aatttctctg agatgttcgc
     5041 aagcgggcca gtcccctgag ccgatatttc ataccacaag aatgtggcgc tccgcggttg
     5101 gtgagcatgc tcggtccgtc cgagaagcct taaaactgcg acgacacatt caccttgaac
     5161 caagggttca agggttacag cctgcggcgg catctcggag attcccttct tatctggcac
     5221 cagccatgac gcaactacca gaactcccac tgacattatc ccgacaagaa atccgcaaaa
     5281 tgattcggca acgtcgtcgt gcgttaacgc cggaacaaca gcaggaaatg ggtcaacaag
     5341 ccgctacccg gatgatgact tatcccccgg tggtgatggc gcatacggtc gctgtattcc
     5401 tctcttttga tggtgaactc gacacccagc cgctaataga acaactctgg cgcgccggta
     5461 agcgcgtata tcttccagtt ttgcatccct ttagtgccgg taatttgctg ttcctgaatt
     5521 accatccgca aagcgaactg gtgatgaaca ggttgaagat ccatgagcca aaactggatg
     5581 tgcgtgacgt gctacccctt tcccgattag acgtgctgat cacaccgctg gtcgcctttg
     5641 atgagtacgg tcagcgcctg ggaatgggcg gtggttttta tgatcggacc ttacaaaact
     5701 ggcagcacta taaaacgcaa ccggtgggtt atgcgcatga ttgtcagttg gtggaaaaac
     5761 tccccgttga agagtgggat atccctcttc ctgcggtggt tacaccgtcg aaagtctggg
     5821 agtggtaagg gcgatacacc tgcatcgccc tgattgacat cgttgattct ttaacctaat
     5881 ttagtgagta agggtaaggg aggattgctc ctcccctgtg actgactgtt aataagctgg
     5941 gaaacttatg agtaacagta caatcagtat gatggcaagt cgcatcataa cccttctcct
     6001 tcaagccctc gcttcggtga gggctttacc gttacagccc catgctgccc agctatcgta
     6061 agctctcttt aaataaccac aacgcattga tctaccttta aatcttttct tcgagcgtgc
     6121 tcgaaaaacc catgcaaaac agagataatt tttgggaaaa aggatctatt acggcagtaa
     6181 acgtgggcga tgaagtggct atcaaaaagt tcggttcagg gcgatgcacc ccgcatcgcc
     6241 ctgattgaca tcgttgattc tttgacctaa tttagtgagt aagggtaaga gaggatgact
     6301 cctcccctga gactgactgt taataagctc cgaaacttat cagtaacagt acaatcagta
     6361 tgatgacaag tcgcatcata acccttctcc tttaagccct cgcttcggtg agggctttac
     6421 cgttacagcc ccatgctgtc ccgccatcgt aaatccccat taaataaaca caacgcattg
     6481 atctgagttt gatttatttt ctggagcaga ctcgcaaagt agaatgcgca acgcggcaac
     6541 ggtgtggaga agggataaaa aaacgggcaa gtcagtgacc tgcccgttaa ttttcagaga
     6601 aggggaatta gtacagcaga cgggcgcgaa tggtacccgg aatagctttc attgcctgca
     6661 gcgctttttc ggcaacgtct tcgtcagctt caatatcaat aactacataa cccatctggg
     6721 cggaagtttg tagatattgc gcggcgatgt tgacgccctg ctcggcgaag attttgttca
     6781 gcgcagttag cacgcccgga cggttttcgt ggatgtgcat cagacgacga ccaccatgca
     6841 gtggcagcga gacttccggg aagttcaccg cagagagcgt tgagccattg tcagaatact
     6901 tgatcaattt acccgcaact tccaggccga tattctcctg cgcttcctga gtcgaaccgc
     6961 caatgtgtgg cgtcagaagg acgttgtcga attcacacag tggagaggta aatggatcgc
     7021 tattggtcgc cggttccgtc gggaatacgt cgattgccgc ccccgccaga tgtttgctcg
     7081 ccagcgcatc acacagcgcc ggaatatcca ccacagtacc gcgcgaggca ttaatcagca
     7141 gcgagccggg cttcattagc gaaatctctt tcgcgcccat catatttttg gtggacggat
     7201 tctctggtac atgcagactc accacatcgc tcatattcag caggtcagaa agatgctgta
     7261 cctgagtggc gttgcccagc ggcagtttgt tttcaatatc ataaaagtaa acatacattc
     7321 ccagcgattc agccagaatg cccaattgcg taccaatatg accgtagccg atgataccca
     7381 gctttttgcc gcgcgcttca aaagaacccg ccgccagttt gttccacacg ccacggtgcg
     7441 ctttagcatt ggcttccggc acgccgcgca atagcagcag cagttcgcca atcaccagct
     7501 ccgcaacaga gcgcgtattt gagaacggtg cgttaaatac cgggatcccg cgctttgccg
     7561 ccgcatccag atcaacctgg tttgttccga tacagaaaca gccaatagcg accagttttt
     7621 ctgcggcgtt gatcacgtct tcagtcagat gggtacggga tcgcaggccg atgaagtggg
     7681 catcgcggat ggattctttt aattgttcat catccagcgc gcctttgtga aattcgatgt
     7741 tggtgtaacc agctgcacga aggctttcca gcgccttttg gtgcacgcct tctaccagca
     7801 gaaacttaat cttgtctttc tccagcgata cctttgccat ttacccaatc ctgtcttttg
     7861 aaatgttgtg tgcggatttg catccgcctt tcaacatatc aaaaaataat attgcggcaa
     7921 tatgaacgtt tgcgtcgcga tgttgaggaa atatcacgca aaggtatttc ttggttgaat
     7981 atgctggttt agaggattta tttagagcaa tcgacaatag ccaagtaaaa acgtgacaca
     8041 tgtcaccaaa tttaatgaag agaatttttt taacggggga ggttcccccg tcagatcatt
     8101 tcacaatggt tttgacaccg tcaggtgtgc caatcagcgc aacgtccgcg ccacggttag
     8161 caaacaagcc aacagtcacc acgccaggaa tcgcatttat ggcgttttcc atcgctatcg
     8221 ggtcaaggat ttccatgccg tggacgtcga ggatcacgtt gccgttatcg gtcaccacgc
     8281 cctgacggta ttccggacga ccgcccagtt tcaccagctg gcgcgccact gcactacgtg
     8341 ccatcgggat aacttctact ggcagcggga atttgcccag aatatcaacc tgcttggaag
     8401 cgtcagcaat acagataaat ttttccgcaa ccgaagcaat gattttttca cgggtcagcg
     8461 ccgcgccgcc gcctttgatc atttgcatgt ggccgttgat ttcatctgcg ccatcaacgt
     8521 agatgccaag gctgtcgact tcgttgagat caaaaacgtg aatgccgagg cttttcagtt
     8581 tttcagtgga agcatctgaa ctggaaacgg ccccttcaat ctggcctttc attgtaccga
     8641 gcgcgtcaat aaagtgtgcg gcggtggaac ctgtacctac accaacaatg gtgccgggct
     8701 gaacatactg aagtgccgcc catcctactg cttttttcaa ttcatcctgc gtcatgatcg
     8761 tttcgcctgt ggtatgaaat ttcacacgca ttatatacaa aaaaagcgat tcagaccccg
     8821 ttggcaagcc gcgtggttaa ctcatccata aaatatcgcg caatggcggg catccccttt
     8881 cgccccgcaa ataaagcata caacggtctg ggtatgccgc tccacggtgc gaacaggcgc
     8941 accagttccc cccgttcgca agcccctgtt tacaggcaaa ttgaggcaat aacgccaccc
     9001 cattcaacac aaccagggca cgaactcgct gagcgaaatg cattagcgca aatccctgat
     9061 cttttaagct gcgcgtttaa ccacggatac aaatgttacc cgccgacggc ttcggtatat
     9121 gcaacctgac acaaaattgt gtcatagtgc aggaaaaagc atttaccagg agcagacaac
     9181 agcaatgaaa cgcccggact acagaacatt acaggcactg gatgcggtga tacgtgaacg
     9241 aggatttgag cgcgcggcac aaaagctgtg cattacacaa tcagccgtct cacagcgcat
     9301 taagcaactg gaaaatatgt tcgggcagcc gctgttggtg cgtaccgtac cgccgcgccc
     9361 gacggaacaa gggcaaaaac tgctggcact gctgcgccag gtggagttgc tggaagaaga
     9421 gtggctgggc gatgaacaaa ccggttcgac tccgctgctg ctttcactgg cggtcaacgc
     9481 cgacagtctg gcgacgtggt tgcttccggc actggctcct gtgttggctg attcgcctat
     9541 ccgcctcaac ttgcaggtag aagatgaaac ccgtactcag gaacgtctgc gccgcggcga
     9601 agtggtcggc gcggtgagta ttcaacatca ggcgctgccg agttgtcttg tcgataaact
     9661 tggtgcgctc gactatctgt tcgtcagctc aaaacccttt gccgaaaaat atttccctaa
     9721 cggcgtaacg cgttcggcat tactgaaagc acctgtggtc gcctttgacc atcttgacga
     9781 tatgcaccag gcctttttgc agcaaaactt cgatctgcct ccaggcagcg tgccctgcca
     9841 tatcgttaat tcttcagaag cgttcgtgca acttgctcgc cagggcacca cctgctgtat
     9901 gatcccgcac ctgcaaatcg agaaagaact agccagcggt gaactgattg acttaacgcc
     9961 agggctattt caacgccgga tgctctactg gcaccgcttt gctcctgaaa gccgcatgat
    10021 gcgtaaagtc actgatgcgt tgctcgatta tggtcacaaa gtccttcgtc aggattaatc
    10081 catcaaataa tgcctgatag cacatatcag gcgttatcct cacttctttt tgtattcctt
    10141 gaatcacatc acaaaataga caaatctcag gcggcaaaaa acgacgtctg aatgcatttt
    10201 tttgctggcg acaaacccac gtaaaaagct caccgtaagc gcaaataccc tcattttgat
    10261 tgcgttttac ggagcaaata atgtctaacg agcaggagtg gcaacagctt gccaacaagg
    10321 aattgagccg tcgggagaaa actgtcgact cgctggttca gcaaaccgcg gaagggatcg
    10381 ccatcaagca gctgtatacc gaagccgatc tcgataatct ggaggtgaca ggtacccttc
    10441 ctggtttgcc gccctacgtt cgtggcccgc gtgccactat gtataccgcc caaccgtgga
    10501 ccatccgtca gtatgctggt ttttcaacag caaaagagtc caacgctttt tatcgccgta
    10561 acctggccgc cgggcaaaaa ggtctttccg ttgcgtttga ccttgccacc caccgtggct
    10621 acgactccga taacccacgc gtggcgggtg acgtcggcaa agcgggcgtc gctatcgaca
    10681 ccgtggaaga tatgaaagtc ctgttcgacc agatcccgct ggataaaatg tcggtttcga
    10741 tgaccatgaa tggcgcagtg ctaccagtac tggcgtttta tatcgtcgcc gcagaagagc
    10801 aaggtgttac acctgataaa ctgaccggca ccattcaaaa cgatattctc aaagagtacc
    10861 tctgccgcaa cacctatatt tacccaccaa aaccgtcaat gcgcattatc gccgacatca
    10921 tcgcctggtg ttccggcaac atgccgcgat ttaataccat cagtatcagc ggttaccaca
    10981 tgggggaagc gggtgccaac tgcgtgcagc aggtagcatt tacgctcgcc gatgggattg
    11041 agtacatcaa agcagcaatc tctgccggac tgaaaattga tgacttcgct cctcgcctgt
    11101 cgttcttctt cggtatcggc atggatctgt ttatgaacgt cgccatgttg cgtgcggcac
    11161 gttatttatg gagcgaagcg gtcagtggat ttggcgcaca ggatccgaaa tcactggcgc
    11221 tgcgtaccca ctgccagacc tcaggctgga gcctgactga acaggatccg tataacaacg
    11281 ttatccgcac caccattgaa gcactggctg cgacgctggg cggtactcag tcactgcata
    11341 ccaacgcctt tgacgaagcg cttggtttgc ctaccgattt ctcagcacgc attgcccgca
    11401 acacccagat catcatccag gaagaatcag aactctgccg caccgtcgat ccactggccg
    11461 gatcctatta cattgagtcg ctgaccgatc aaatcgtcaa acaagccaga gctattatcc
    11521 aacagatcga cgaagccggt ggcatggcga aagcgatcga agcaggtctg ccaaaacgaa
    11581 tgatcgaaga ggcctcagcg cgcgaacagt cgctgatcga ccaaggcaag cgtgtcatcg
    11641 ttggtgtcaa caagtacaaa ctggatcacg aagacgaaac cgatgtactt gagatcgaca
    11701 acgtgatggt gcgtaacgag caaattgctt cgctggaacg cattcgcgcc acccgtgatg
    11761 atgccgccgt aaccgccgcg ttgaacgccc tgactcacgc cgcacagcat aacgaaaacc
    11821 tgctggctgc cgctgttaat gccgctcgcg ttcgcgccac cctgggtgaa atttccgatg
    11881 cgctggaagt cgcttacgac cgttatctgg tgccaagcca gtgtgttacc ggcgtgattg
    11941 cgcaaagcta tcatcagtct gagaaatcgg cctccgagtt cgatgccatt gttgcgcaaa
    12001 cggagcagtt ccttgccgac aatggtcgtc gcccgcgcat tctgatcgct aagatgggcc
    12061 aggatggaca cgatcgcggc gcgaaagtga tcgccagcgc ctattccgat ctcggtttcg
    12121 acgtagattt aagcccgatg ttctctacac ctgaagagat cgcccgcctg gccgtagaaa
    12181 acgacgttca cgtagtgggc gcatcctcac tggctgccga tcataaaacg ctgatcccgg
    12241 aactggtcga agcgctgaaa aaatggggac gcgaagatat ctgcgtggtc gcgggtggcg
    12301 tcattccgcc gcaggattac gccttcctgc aagagcgcgg cgtggcggcg atttatggtc
    12361 caggtacacc tatgctcgac agtgtgcgcg acgtactgaa tctgataagc cagcatcatg
    12421 attaatgaag ccacgctggc agaaagtatt cgccgcttac gtcagggtga gcgtgccacg
    12481 ctcgcccagg ccatgacgct ggtggaaagc cgtcacccac gtcatcaggc gctaagtaca
    12541 cagctgcttg acgccattat gccgttctgt ggtaacgccc tgcgactggg cgttaccggc
    12601 acacccggcg cggggaaaag tacctttctt gaagcctttg gcatgttgtt gattcgtgag
    12661 ggattaaagg ttgctgttat tgcggtcgat cccagcagcc cggtcactgg cggtagcatt
    12721 ctcggggata aaacccgcat gaatgacctg gcgcgtgccg aggcggcgtt tattcgcccg
    12781 gtaccctcct ccggtcatct gggcggtgcc agtcagcgag cacgggaatt aatgctgtta
    12841 tgcgaagcgg cgggttatga cgtagtgatt gtcgaaacgg ttggcgtcgg gcagtcggaa
    12901 acggaagtcg cccgcatggt ggactgtttt atctcgttgc aaattgccgg tggcggcgat
    12961 gatctgcagg gcattaaaaa agggctgatg gaagtggctg atctgctcgt tatcaacaaa
    13021 gacgatggcg ataaccatac caatgtcgcc attgcccggc atatgtacga gagtgccctg
    13081 catattctgc gacgtaaata cgacgaatgg cagccacggg ttctgacttg tagcgcactg
    13141 gaaaaacgtg gaatcgatga gatctggcac gccatcatcg acttcaaaac cgcgctaact
    13201 gccagtggtc gtttacaaca agtgcggcaa caacaatcgg tggaatggct gcgtaagcag
    13261 accgaagaag aagtactgaa tcacctgttc gcgaatgaag atttcgatcg ctattaccgc
    13321 cagacgcttt tagcggtcaa aaacaatacg ctctcaccgc gcaccggcct gcggcagctc
    13381 agtgaattta tccagacgca atattttgat taaaggaatt tttatgtctt atcagtatgt
    13441 gaacgttgcc actatcaaca aagtggcggt cattgagttt aactatggac gaaaacttaa
    13501 tgccttaagt aaagtcttta ttgatgatct tatgcaggcg ttaagcgatc tcaaccggcc
    13561 ggaaattcgc tgtatcattt tgcgggcgcc aagtggatcc aaagtcttct ccgcaggtca
    13621 cgatattcac gaactgccgt ctggcggtcg cgatccgctc tcctatgatg atccattgcg
    13681 tcaaatcacc cgcatgatcc aaaaattccc gaaaccgatc atttcgatgg tggaaggtag
    13741 tgtttggggt ggcgcatttg aaatgatcat gagttccgat ctgatcatcg ccgccagtac
    13801 ctcaaccttc tcaatgatgc ctgtaaacct cggcgtcccg tataacctgg tcggcattca
    13861 caacctgacc cgcgacgcgg gcttccacat tgtcaaagag ctgattttta ccgcttcgcc
    13921 aatcaccgcc cagcgcgcgt tggctgtcgg catcctcaac catgttgtgg aagtggaaga
    13981 actggaagat ttcaccttac aaatggcgca ccacatctct gagaaagcgc cgttagccat
    14041 tgctgttatc aaagaagagc tgcgtgtact gggcgaagcg cacaccatga actccgatga
    14101 atttgaacgt attcagggga tgcgccgcgc ggtttatgac agcgaagatt accaggaagg
    14161 gatgaacgct ttcctcgaaa aacgtaaacc taatttcgtt ggtcattaat ccctgcgaac
    14221 gaaggagtaa aaatggaaac tcagtggaca aggatgaccg ccgatgaagc ggcagaaatt
    14281 atccagcata acgacatggt ggcatttagc ggctttaccc cagcgggttc gccgaaagcc
    14341 ttaccgaccg cgattgcccg cagagctaac gaacagcatg aggccaaaaa gccgtatcaa
    14401 attcgcctgc tgacgggggc gtcaatcagc gccgccgctg acgatgtact ttctgacgcc
    14461 gatgctgttt cctggcgtgc gccatatcaa acatcgtccg gtttacgtaa aaagatcaat
    14521 cagggcgcgg tgagtttcgt tgacctgcat ttgagcgaag tggcgcaaat ggtcaattac
    14581 ggtttcttcg gcgacattga tgttgccgtc atcgaagcat cggcactggc accggatggt
    14641 cgagtctggt taaccagcgg gatcggtaat gcgccgacat ggctgctgcg ggcgaagaaa
    14701 gtgatcattg aacttaatca ctatcacgat ccgcgcgttg cagaactggc ggatattgtg
    14761 attcctggcg caccaccgcg gcgcaatagc gtgtcgatct tccatgcaat ggatcgcgtc
    14821 ggtacccgct atgtgcaaat cgatccgaaa aagattgtcg ccgtcgtgga aaccaacttg
    14881 cccgacgccg gtaatatgct ggataagcaa aatcccatgt gccagcagat tgccgataac
    14941 gtggtcacgt tcttattgca ggaaatggcg catgggcgta ttccgccgga atttctgccg
    15001 ctgcaaagtg gcgtgggcaa tatcaataat gcggtaatgg cgcgtctggg ggaaaacccg
    15061 gaaattcctc cgttcatgat gtattcggaa gtgctacagg aatcggtggt gcatttactg
    15121 gaaaccggca aaatcagcgg ggccagcgcc tccagcctga caatctcggc cgattccctg
    15181 cgcaagattt acgacaatat ggattacttt gccagccgca ttgtgttgcg tccgcaggag
    15241 atttccaata acccggaaat catccgtcgt ctgggcgtca tcgctctgaa cgtcggcctg
    15301 gagtttgata tttacgggca tgcaaattca acgcacgtag ccggggtcga tctgatgaac
    15361 ggcatcggcg gcagcggtga ttttgaacgc aacgcgtatc tgtcgatctt tatggctccg
    15421 tcgattgcta aagaaggcaa gatctcaacc gtcgtgccaa tgtgcagcca tgttgatcac
    15481 agcgaacaca gcgtcaaagt gatcatcacc gaacaaggga tcgccgatct acgcggtctt
    15541 tccccgcttc aacgcgcccg cactatcatt gataattgtg cacatcctat gtatcgggat
    15601 tatctgcatc gctatctgga aaatgcgccc ggcggacata ttcaccacga tcttagccac
    15661 gtcttcgact tacaccgtaa tttaattgca accggctcga tgctgggtta atggtttgtc
    15721 ccgtcacgat cttcagcaat atgctgaagc atcgtggcgg tataacgatg atttctgatt
    15781 gaataaagat attcctgcat atacatcggc ctgtgcggcg catcaaaata tttcagcgtt
    15841 gccggattca ccagtcgcca ggcaaaatgc gggatcaccg tcagaaaatg tccgcgctcc
    15901 acggcgctga ttttggccat aaaactgtag ggacgataaa taatggtggg attgatccca
    15961 caaggtcgca tattcgcatc aatcattgct tcgaagttag cccggttttg aaagcgcatt
    16021 tgtagccagg gcagctcctg aagcagctct tgctcttgac gctcttcata acggcgagaa
    16081 acgagaaagc ccaatcgcag cggtggtaat tcactgatag tcaggttttc cagttcctga
    16141 actcgtgcag atacatgctg tggggagata ataaaatcta actgacggtc aaaaagatta
    16201 tcgataacgc cgttttcgct gaactcaact ggctgggcgg tcacgccttc atacttatct
    16261 ccgagactaa tcaactgatc gaaaataatt gtcggataag tattatctat gccaatcaca
    16321 att
//
LOCUS       DAZPJP010000080        16272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPJP010000080 DAZPJP010000000
VERSION     DAZPJP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.080"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHU9J_002365"
     CDS             complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHU9J_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR1905882.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHU9J_002366"
     CDS             complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHU9J_002366"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR1905883.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2358..2432)
                     /locus_tag="ACHU9J_002367"
                     /pseudo
     CDS             complement(2358..2432)
                     /locus_tag="ACHU9J_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2648..4030)
                     /locus_tag="ACHU9J_002368"
     CDS             complement(2648..4030)
                     /locus_tag="ACHU9J_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1905884.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4040..4990)
                     /locus_tag="ACHU9J_002369"
     CDS             complement(4040..4990)
                     /locus_tag="ACHU9J_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR1905885.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5133..6551)
                     /locus_tag="ACHU9J_002370"
     CDS             complement(5133..6551)
                     /locus_tag="ACHU9J_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1905886.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHU9J_002371"
     CDS             complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHU9J_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR1905887.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8088..8951)
                     /locus_tag="ACHU9J_002372"
     CDS             complement(8088..8951)
                     /locus_tag="ACHU9J_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1905888.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8991..9596)
                     /locus_tag="ACHU9J_002373"
     CDS             complement(8991..9596)
                     /locus_tag="ACHU9J_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR1905889.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDYTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9854..10351
                     /locus_tag="ACHU9J_002374"
     CDS             9854..10351
                     /locus_tag="ACHU9J_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1905890.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10443..11375
                     /locus_tag="ACHU9J_002375"
     CDS             10443..11375
                     /locus_tag="ACHU9J_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1905891.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11417..12505)
                     /locus_tag="ACHU9J_002376"
     CDS             complement(11417..12505)
                     /locus_tag="ACHU9J_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1905892.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12647..>16272)
                     /gene="ehaA"
                     /locus_tag="ACHU9J_002377"
     CDS             complement(12647..>16272)
                     /gene="ehaA"
                     /locus_tag="ACHU9J_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR1905893.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3774 a         4392 c         3855 g         4251 t
ORIGIN      
        1 actgctggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg gcgaaaaggc
       61 ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct ttgcgattca
      121 ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga
      181 ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact ctattagata
      241 tccattcatt taatcgtgca tcatctctgt atgtttttac gtccagaaca gagagcaata
      301 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
      361 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
      421 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
      481 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
      541 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
      601 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
      661 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
      721 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
      781 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
      841 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
      901 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg
      961 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
     1021 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag aagtattgtg
     1081 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
     1141 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
     1201 acagcgtatt tattaatttt ttcaatctgt tagtgtgcga ttatcgataa caaaacgata
     1261 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
     1321 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
     1381 aatgccgcca atcatagaat cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
     1441 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
     1501 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
     1561 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
     1621 cagggctttt tccgcttcgc gttttgcctc agaagtggta aatgccacca catgtgcccc
     1681 catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga ccacgcccac
     1741 ttttttaccc ggcccggcct gccagtgacg tagcggcgag tacgtggtga tccctgcaca
     1801 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
     1861 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
     1921 cggcgagtta taggtgccga tcatgtgatc acagtagttt tccagcccgt cctcacactc
     1981 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
     2041 tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
     2101 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
     2161 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
     2221 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
     2281 tcctgattag ctatgtgtat ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca
     2341 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
     2401 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
     2461 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
     2521 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
     2581 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
     2641 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
     2701 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
     2761 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
     2821 cagtggctag aaacagcgaa cgggagaggt tctgttcgtt aggacgaata tagagctgcg
     2881 cgtagagatt ggctttttcc agcatgtcgc ccagaccata aggcgaccag tggtcgataa
     2941 cgctgtcagt gccagtcatc acttttacgc ctttgtcgtg caactgtttg agcggcatat
     3001 gcagcgtgcc aatcggcacc gtcgaggcga tagaaatttg ctgcgccgcc atccgattcg
     3061 ccagcgcatc gacctgttgt tcgttgagcg tagccagcgc aaaggcgtgg ctgatggtca
     3121 gcttaccttt cagttgcggc gttttctcta ccgtttcaac catataattg atggctgcca
     3181 cgcccgacgg agtggtttcg tgcaggtgaa tatcgacacc tttgtcgtag tccagcgcaa
     3241 tctgaaacat ggtgtcgagg gatttttcca tcgcgccatc gacactggtc gggtccagcc
     3301 cgccgacgta atgcgcaccc gcctgcatcg cttcgcgcat taagggttcc gatttcgaca
     3361 gcagcaaacc gtgctgcggg aaggcgacga tttcacactc gaagcccgcc tgacgccgcg
     3421 ccagcaccgc ctgcaaattt tgcagatttt ttaggccgga aaccggttcg atattgcagt
     3481 ggctgcgggc aatggtggtg cctttcgact gcaatagatc gatcagtttt tccgcccgct
     3541 cctgcgtata cggttgtaac tccggcagca ttttctgctt gagtttgatc atgtcctgga
     3601 tagtggtgcc tgccggacga ttgagcgagc gccacggccc gccgtagaag gttttgtcga
     3661 gatgaatatg catgtcgcga gtggtgggta gcatcaactt accgccagcg tcatagtgcg
     3721 gcagcgtggc gtccggatgc tgcttgttct cgcgcagggc gacaattttg ccgtcctgaa
     3781 tctccacggt ctggcgcgcc gtgcgggtct gcaccgccac accgttttcg tagtcaaaac
     3841 cggtttccag cagcacgtta tcgagatagt aatgcgggtc agtgattttc atggtgttgt
     3901 tcgcttcgca gtttagggtg ccagctaccg ccgcatgggc gagcggcaca taggtaccaa
     3961 acagcgcggc ggcggtgacc atcttaccgc tctggctcag aaattcacgg cggctattgc
     4021 tttctttcat cctacgtcct tacgttttaa taatatgggt gcccgtttca ccgcgcagcg
     4081 ccgcaggcag gcattccggc gtggtgataa tcacttcttt gccgccctgc tccaggaacg
     4141 tcaggctggc gatgattttt ggcaacatgc tgccgggaga aaaatgccct tcctgcatat
     4201 agcgagtcat ggtggcaata tccacccgat ccagcgcctg ctgctgcggt ttaccaaagt
     4261 ggatacagac tttttcgacg tcggtggtga tcaccagaat gtcggcgtga atttcacggg
     4321 ccagcagcgc ggtagagaga tctttgtcaa taaccgcgtc cacgctttgg taatcccccg
     4381 cttcagtacg cactaccgga attccaccgc cgcccgcgcc aattacgaca aaaccttgtt
     4441 ggatcagcgc cttaatggcg ggtgcttcga caatacgttt cggttccggc gaggcgacca
     4501 cgcgacgata gccccgtccg gcatcttcaa caaaacacca gtcagggttt gccttttgta
     4561 gtttgtcacg ctggctttca ctgaagaatg caccaatggg cttggtggga tgggcaaaac
     4621 ctggatcgtt tttatccact tccacctgag ttaccacggt gacggctttt ttctcgccgt
     4681 gacgcgccag tcggttattc agcgcctgtt ggatcagata gccgatgccg ccctgcgtat
     4741 ctgccacaca gttcgccagc ggcgttaagg gcagcccttc gcgctcgtgg gcaatctccg
     4801 cacggcgtaa atccagcccg acctgcggcc cgttaccgtg ggtcagcaca atgtcgtaat
     4861 cggaagccag catttccagc accgtatcgg ccacggcttt caccgcctcc gcctgatgct
     4921 caatcgactg gctggcgtta tctttgataa tgctgttgcc accaatggca acgaccacaa
     4981 gctctttcat gttgttatgt ccgctacaga gggtgatgag gttatgacca acgccagact
     5041 gcctgatgcg ctacgcttat caggcctaca attctcgggc catccgtagg ccggataagg
     5101 cgttcacgcc gcatccggca taaacatgta agtcagacgc cgtaacgttc gcaccagccg
     5161 agaatggctt tctcgaagca agcgaatggt ggatgcacaa tgcccgcgcc aatcatgccg
     5221 acgcccgcat ctttatgggc gatggcggtg ttgatcaccg gcagaatgcc gctgctaccc
     5281 acgcgggtga tgtcgattgc cgacggcacg cccataaaac cgagcagcgg aatggtgacg
     5341 ttggggtttt caccgagagt gatttcacgc atctgacggg agaaatcgat agcctcttcc
     5401 accgtgccgc ccaccagcgc gacgattgca ggcgcggtcg ccatagcaaa tccaccaata
     5461 ccgtaggttt cggtgatggc gctgtcgccg atatccagcc ccgaatcttc cggcttataa
     5521 ccggcaaaca tcgggccgat cacctgctgc gccgggccgg taaaccattg ccccggcagg
     5581 ccgctgaccc gcaggccgaa ctcgacgccg ttacgcgcca tggtggtgac cacggtgctg
     5641 tattcgatgc cgtgcgccgc atccatcgcc gctttacaca tcgccatcca cgtcgggccg
     5701 gagaagtagt cgctgctggc gacaaactcg aacacttcgc gctgttgctc gacggaatag
     5761 cctgcctgaa tgatccccgg cgtcagtgcc tgaatcagca gcgtcgtccc ggcgttattg
     5821 cggttatggc actcatcgcc catatgcagc gcctgcgcca gcattaagcg cagatcgatc
     5881 tcaccgataa ttttcatcgc gtcgcgcagc attggcccct gcacatcgcg catccagttg
     5941 aggcggtcga tcacgctctg atcgttggca cccatacgca aaatcttcgc catctgctcg
     6001 ctcatattgg tgtaagcgat gttgccgtag gttttgtttt tcacgatgtg cataaacatc
     6061 gaggccgagg taacgcccgc catcgatccc acacagtcgt gttcgtggca cggcgagaag
     6121 gtgatctccc cggaggccgc cagttcagcc gcctcgtcga gatctttcgc cagtccttcg
     6181 aacaccagcg ctccggtgac cgcgcctttc atcgcgccgc acattttttc ccaggtgatc
     6241 ggcggcccgg cgtgaagaat ggttttcggc gtcatgcccg gcaccacgtt aatcgcctga
     6301 tcaaaaccaa tcagcaccgg atgcgactgg ataatgcgct ccagcgcctg ctggttagcg
     6361 gcggcgattt tgtctgccag cggcgaatcg gcaatgttat ccagcgcctg caccacctgc
     6421 atattgccct gccccggcgg cgtccagtcg agttgagtca cttctacatg ctgctttttc
     6481 aggtcatcgc taaacatggc gatgccgacg ttaataacgt tcaatggttg gctaaacagt
     6541 gactggctca tcaggcttcc tcccctttgc agataaattc acgcgccagc aatccggtat
     6601 tggtgctgct gctcgccaga atcactccgg catcaagcag catctggctt tgttgttcta
     6661 acgatggcgt atcaagatcg gtacccagca cataggcgag aatgatcaac tcgcgtcctt
     6721 cggcagcggc gatcgctttc gcttctttga tcgcctcgat ggtggagccg accggatctt
     6781 catgcgatcc aaatccgagc acaaaatcca tcacgatcac cgccacttct ggatcgcgcg
     6841 cctcttcgat caagcgactg atgcggttgg tggggtcaat cattggatgc ggcttgccgt
     6901 tcgtgaagtc gtcatcgcca aagtcgagga aggtgtgttt gatgctgcgg ttgatatctt
     6961 gcaggcggaa ttccggatcg ggctgaatgt tgctgtagac atcgccatgt ttttccatca
     7021 ccgcgaacat ggtttcgtcg cacagcgtgc cgccgcagaa caggccacga atgtatttct
     7081 gctgcggttg cagacgcgca cgcacatcag caatcaacgg ctggttaagc gtatgcaggt
     7141 cgagattttc ctgtttcacg ccggagagca tcaccgcttt tagcgccgcc tctttgctgc
     7201 cgcgggcaaa ctgtagcccc tgctcatcca ccggcgtttc aacgcgaccg aggaagcaga
     7261 ccaccaccgg cttgcggcag gcacgtgcgc gttccagcac tttgcgggcc accgcaggcg
     7321 cgggaggttt tgaaatcagt gcgataattt cagtttgcgg atcgttttcc agcatcccga
     7381 tggcgtcgag catcatcagg ccgccgattt tctcgctcag gtcgcgcccg ccggtgccaa
     7441 tcagttgtga aacaccgcca ccaaattcat gaatgcggac gctcagctcc tggctgccgg
     7501 tgccggatgc gccaacaata ccgatattgc cgcgacgcac ggcgttacca aaacagagcg
     7561 ccgcgccgtt aataatcgcc gtgccgcagt ccggccccat catcagcagc cctttttcgt
     7621 gggccaactg cttgagcgcc agttcatctt caactgagac gttatcggaa aacagcatca
     7681 cgttgagatc gttttgcagc gcctgacgcg cttcgcgagc ggcaaacaga ccgttgaccg
     7741 aaatcaccgc caggttactt tccgggatat gctttttggc gctggcaata gtggcgtaac
     7801 gcgcctcgtg cgagccgctt tgcgctttgg tgttgaacag ttcttcaatc tccaccagca
     7861 actgctcgtt gtccgcaccc gatttaccat tgatgacaat catcaggtcg ccgtttttcg
     7921 cctgttccag cgtcggcgtc agcagtccta aatttttcag cacacctttg ttcatttcgg
     7981 tcgccatcgc cacaaatgcc tgctcgacgc cgtcgagttt atttgcacgc gtggagatag
     8041 acatcagcga gacagaatca aaataggtat tcggtttaat gactatttta actgacataa
     8101 ttccctccgt aggtttggct cagcgcacaa ccatcggcca tgccatacag catgtcgcag
     8161 ccggaactgg agccaatatg tttaattttt tctattgcct gagttgaatt cccagggacg
     8221 ccataaataa tgttgtgaat aaagcgatga atattttccc ggcagcgttg ttgtaatgcg
     8281 gcttccagcg ttatggcact taataacgtg gtgttatttc tgccgcgtag cagaccgcga
     8341 taaaattcct ctttgtattt ttctgccgga tgcccgggaa taaataaaat aagcgccaga
     8401 cctgttaaat aatcatcggc tgatggcgta aggccaatcc ctaacccaat catactggcg
     8461 accgctgcgg cgatattttg cttatcgttt actgtctgaa taactgcccg tcgtttaata
     8521 tgtaattctt tatttaacgc ctgataaaac ggattatcgc cgcgataatt aaacagggtt
     8581 tcattttccc ccaggtgctg atgaataatg tcgtgccact gtagccaggc gataaggtga
     8641 aaattcacag cggtcatttg caaggttggg gacagccaac gccgacaacg actcatctct
     8701 atatgaagat gttgaccaac cgtaatacct tgatcgtcaa actgaacctt atcaccatgc
     8761 cggaacagat catcaaagtg agtgagtgcc aaccgacaac tgtttggcgc attgtcgtac
     8821 tcttcacaaa gcagcgtcag caactgctgg cgagccggga gtgcgatatt taccgctctg
     8881 gcaaagacct gttcaacctg tccttgcccc cgctgcgcca gaaaatccgc atccgcagtt
     8941 agcgcccaca cacaccgttt ttctggtgtg ttgatagcca gcctcccggt ttatgcacct
     9001 gcggcaatca gtaactgcgc aatctcttca aagccccgtt cccgcgccag ttccagtggc
     9061 gttttgccat atttatcggt cagatgcggg ctggcaccgt gttccagcaa taactgcaca
     9121 atcgcctgct gtttaatacc accatcatta agcacaatcg cttccagtag cggcgtccag
     9181 cctacatggt tggtctggtt aacgttaatc tccgtatgcg ccaaaagctc ttttacaata
     9241 cttaaatggc ctttttcaca ggcaggcgtc aggccgacac cgccaaaacg ggttacgcaa
     9301 ttaagatcgg gtttagccgg taaaataatt cgtagtagcg ttaaatcatc gttcagacag
     9361 ctaattaaaa aaggatttaa acaggtataa tcttgcttat taatatccgc tccggcatca
     9421 attaatgcct gaacgcaagc atattgctga tataaacttg ccagcgtaat tgccgttttc
     9481 ccctgacgat cgcaggtatt aatatcgaca cccagcgcaa ggcaggtttt tactttatcg
     9541 atatcaccct gttgtgcagc caataaatag tcggcaggta gattttttat actcattcac
     9601 attcccctgg tttttgtggc tgttttcgat gaattgagaa tagcaacgat aatctgataa
     9661 aagaacccgc ttaaatttaa ttcgccgtat ctcgcctttt tgttgaataa tattcaacaa
     9721 tcttttcgca gtgtgcggta gtgcaatatt tgtttctttg acccgatacc ctttcactca
     9781 atcaggtttt atttctctat agtctgcaca tgtaagagta gctttcgtcc atgtctaacc
     9841 agaggggaac aagatgaacg gactcacggc aacgggtgtc accgtcggta tttgtgcagg
     9901 gctttggcag ttagtgtctt cacatgtcgg gctctctcaa ggatgggaat tattaggaac
     9961 cattggcttc gtcgcctttt gcagttttta tgccgcaggc ggcggtaaat cggggtttat
    10021 cagaagtctg gcggttaatt attccgggat ggtgtgggcg tttttcgccg cgctgactgc
    10081 tggttggctg gcctctgtaa gtggactgtc cgcgttctgg gcgagcgtaa ttactaccgt
    10141 acccttctcg gcggtagtgg tctggcaagg gcgtttctgg ttactgtcct ttattcccgg
    10201 tggctttctc ggcatgacgc tgttttttgc cagcgggatg aactggacgg tgacgttact
    10261 gggttttctg gcgggtaact gcgtggggat aatttcagaa tatggtggac aaaaattaag
    10321 cgaagcgacg actaaacgcg acgggtatta aaagttattc aaaaatgatt atctatcttt
    10381 aaccgatacc tctacagtca tagctggaga aattcatagt agataatggc gagaatggtt
    10441 taatgaactc aatttttacc gaagagaatc tgttggcatt tactaccgct gcgcgttttg
    10501 gcagcttcag taaagccgca gaagagttgg gtttaaccac ttccgccatt agctacacca
    10561 tcaagcgtat ggagacgggg ctggatgtgg tgctgttcac tcgcagtacc cgcagcattg
    10621 agttaacgga gtccgggcgt tattttttcc gcaaagctac cgacctgctg aatgattttt
    10681 atgccatcaa acgcagtatc gatactattt cgcagggcat agaagcgcgt gtgcgcattt
    10741 gtattaatca gctgctttat acgcccaaac acaccgcacg cttgttacag gtgctgaaaa
    10801 agcagtttcc tacctgccag attaccgtca ccaccgaagt gtataacggc gtctgggatg
    10861 cgattattaa taatcaggcc aatatcgcca tcggcgcacc cgatacacta ctggacggcg
    10921 gcggtattga ttacactgaa attggcgcga ttcgctgggc ttttgctatc gccccggatc
    10981 acccgttagc ctttgtgccg gaacccatcg ccgaaagcca actgcgtctc taccctaata
    11041 ttatggtgga ggacaccgcg catacgatta ataaaaaagt gggctggttg ctgcacgggc
    11101 aggagtcaat tctggtgcca gatttcaaca ccaaatgtca gtgccagatt ttaggtgaag
    11161 gaattggttt tttgccggat tacatggtcc gtgaggcgat gacgcaatcc ctgctggtta
    11221 cccgacaaat ccataatccg cgccaggatt cgcgcatgtt actggcgact cagcattctg
    11281 ctacgggtca ggtgacgcag tggattaaaa aacaatttgc gccaaatggc atattaacgg
    11341 ggatctatca ggatttactg catcgggaga attaatcgct gccaacctcc cgatgcgctg
    11401 tgaatagcgg tttacatcaa ccacctgctt tcattaccca ttcagagata aatttattac
    11461 ccggtactgg cggagagtac aaatagccct gcaaaaagtg aacgccttta ccgatcatta
    11521 aatccgcctg ctcctgggtt tctacccctt ccgccacgat actcagacca ggcttacgcg
    11581 ctagttcgac aatattgtcc acaatatgac cggagatttc gtcgacactc gccatttgca
    11641 caaatgactt atcgatctta ataaaatcga ccgggaacgc ctgcaagtaa cgataggtcg
    11701 cataacccgt accaaagtca tccagcgcaa aggtaatgtt gtgctgatga aggctgtcaa
    11761 atatcgctct ggcttctggc gttaccggaa tagggttacg ttccgttagc tcgagaacca
    11821 gcttgatttt atcgttacct aatttcttaa ccaggttcag acactctttt tcaaatcccg
    11881 ctgccaaaaa acaacccgcc gagacgttga tgccaatatg gaaattgtcc ggcagcaaat
    11941 gttttaccgg catcagaata tccgcagtct gtttcatcag ttggcgggtc attatgacaa
    12001 taagaccgga tgactccgcc agaggaataa actgatccgg tgggataatt cccgtttgtg
    12061 gatgttccca gcggacaagt acctcacagc ccgtcagtac cccagtctgc gcgcagaaaa
    12121 ccggttggat ccacggtttg aattcgtggt tttccaacgc cagactgatg gcttcaggcg
    12181 tgacgatatg gtgacaacgg ttatcaggga tataaaaatt atttttattt cccgtgccac
    12241 tgatgacttg cggatgatat tgaaaaaaga tatcccgcca gaaggtaata tcactctgga
    12301 tgcctagttt ttcatacagc tgcttctttt gatgtgaaat tgtctttgca ctgcgatttc
    12361 tgtactgtga gatttctgta accgacattc cctccattag cagcttgagt aaaagccgtt
    12421 ctttttcgga cacgatacca ggcaacgtca aattcaccgt tgccccgaac tcttccaaaa
    12481 aaacactcaa atcatgcgaa gccattgaac acaccttgat taaatcttgt gcgccagcta
    12541 aatctgcgag tcttcactct gactgataaa gctggctgca agaaaatgac acagagagcc
    12601 gaattatctc ccgttcggct ctctctcctt ttactcatta ttcagatcag aattgccact
    12661 taatgccaac cattgcagag gtgtcattat atcccctatc ggcaacctga acgcccacat
    12721 tcccccagac attcaggttg gcatttagct gtccttccac cccggttttt atctcagcaa
    12781 tatttcgggc tccatcctga gtgacggaca cgccatccat actggtgctg aaatccttac
    12841 tgttatgcag ccagttcact tctacaaacg gctggaactc gcgggattta ccgtcatcca
    12901 ttttatggtg gctcttcagc caggttttta cgccaagtcg ggtttgaaca ttgccatcac
    12961 cgttgctatg aacgagggtt ccgttgcttt cgcggtgctt atcggctttg actcccatcc
    13021 aggtaacctg tgcttgcggc tgaacatacc attcattacg cgttccctgg ctgccattaa
    13081 attcagctaa tttgtgtttg tatccagctt ccagtgaagc ggtaaatcct tttgatttat
    13141 aggattcact ttgtaagtca tcccctttca ctgtgttatc aaaccagctg tactgcgccc
    13201 aactgtcgag atacgcgcca ttacgcgact cgtcatcggc ataccaggtg gcatagaggc
    13261 cggtgctata tccgttcaca ctcgcttttg cacgataacc ggttcgcgag gaaatggttt
    13321 tgctgtcgct gttgccatat cccgccatga ccccaacatg ccagctgtcg ctgccgtttt
    13381 ggctccactg cgcgacatcg cctcccagtt gcagaacata gcgattgctt tgggttttca
    13441 gctggccgct gccatcacgc catttattat gaccaccttc atggcgcatc cacatagtgg
    13501 tttgtttctg ctcacccgtc accatgtcgg tatagtacgt attacccaga cgctcatgca
    13561 gacgcgtggt gaacattgta ttcgctgctg caaggttcgc aatgtagcta cccgcctccg
    13621 gtcgcaggtc attatccaca ttcagatccg gacccggcgt aggtgtcggg ttagggttcg
    13681 gctctggatt cgggtttggc tccggattcg gcatcgggtc tggctccggc tgcagttcag
    13741 gagaatcact gccgctggtc agataccagt taccactatt tgccccttgt ccacgcgcga
    13801 gagtgtagtc atacgccccc gcaacgatac gaccagcctg aacaaattcg ccctcagact
    13861 taccgtctac atggataagt tcgataccgt taagggtttt cgcacctgta ccgccagcgt
    13921 tattcaccgt aacggcagtc gtgccgctag tatcgccctc gacaaccaat ttatcggtta
    13981 ctgagtcatc gccattcagt accgtattca ttactagttg accgttatta ccgtggtagt
    14041 tccccttcac cactaacaca ttgccagctt gctgtcctga cattccggtc atcagcgtac
    14101 cgttgttggt cagattgccg cccagggtgt aattcacaga cgcagccgct cttttttcag
    14161 cgctattatt caacgccagg gtaccgttgt tctcaacgtt tgcgctaccc aatccctgcg
    14221 catgacttac caggacatta gccgaattag caatgttcac attcgctgtc aggttgctgt
    14281 tatcgcccgc gatgtccagc gtgccgccct gaatattcag ctctccgctc cccgttaacg
    14341 aaccggttga agtgcccccg ttggtaaccg tcagactgcc gccatttaag ctcagcagtg
    14401 aatcggcgct gccattgagc gtgcccacgg tctggctgtg accattcata tccagttcag
    14461 tctctgccgc cagacgcagt tcatgggtat tacccaacac gttatcgtta ttcaacaaaa
    14521 gcgtcccact gcgcagatcg gtaacaccgg tatagtcgtt gtctttgtta gaaagcgata
    14581 cggtctgacc cttctggctg ctgaatgcca ggtcaccact gccggtaatc tttgcgctga
    14641 gatcggcggc attctcgctt ttaccgttag agctcagcac cagagcatcg ctgtcggtag
    14701 cgtgtaaatc aagctgggtc aggccgtaac cgatatacag accgtcattg tttaccccac
    14761 tcgtcagacg atagtcgtaa gtgccgacgg ccgctttttg cccattctgc tgaatatcac
    14821 gttgggcact gctggagagg atttgcccat ttttatcctg caattgcaga ttgcccgcat
    14881 cgcccagaac ttccgcacct tgcgcatcaa ccagcttaat ggcgctgtta ccatcatcga
    14941 cttcagtgag cgataacgca gagtcaatat cacgagatac tgagcggaca acgtcactgt
    15001 cagaaacctg aatcacaccc tcgccgcgca aatccagcgt tttactaacg ttgaccgtcc
    15061 cttctgtcat ctgtgcacct gctgtcaggg caccaaaatc aacggttccg ccggagaatc
    15121 ccaaagtgct aatggagtga gcgcccttac ccactgttac caggcttccc tgacccgcag
    15181 tgatcccgcc tctctgcaga aactcagcat tcttttccag gttaaatgtc gtgttctgga
    15241 acgccagtga accctggaac cagtctgtat aagcaaactc atcgaaggag aattcgtgat
    15301 tccccatgtt tatttcaaca atacctctac cttgaagatc gttatcaaat acccaatctt
    15361 tattggcatt gatttgcagt ttgccatcaa catccaggtc cgcacggccg atgttcttgt
    15421 cttcagaaat aaccgcgaca gcaccagaag cgacatcaat atatccgctg aatgcatagt
    15481 tattgccggt cagcgccact gtcccactgt cgatacttat tgtaccgttg ccggaaatct
    15541 tattggcaaa ggtcatgcta tcgtcagcct tcaggtgtaa tttcccgcgg ttgttgattt
    15601 ctgcggtacc tatattttgg gtgatcccta aagcgccatt ttgctcaatt ttgactttac
    15661 cgctaaaggc ggagttatcc ccggttaagt aagtcgtacc attgagcgca ttgatatagc
    15721 ccaggccctc gctgtcactg acaatatccg cggaaatagt cgcgttttta tccgtgtggt
    15781 ttaaggtgat tttcttctcg gatgtctttg cccacacaaa ctcaattttt tcggcatcaa
    15841 taatccctgc agccttcgct gcgcttcctt cctgtgcacc taacgctaac tcagagttgg
    15901 tgcttaaact aatagtcggc gcagagattt taccaccttc cgaaacagca agtgttgtat
    15961 cggagttttg atcataataa cccaggctaa ttttctcact ggcattaact tcaccgccgt
    16021 tattaactga caggtactga ttaccaccgt aactgccgta aacgcccata aagaaggttt
    16081 tagaatttac ctggctgcct tcaccactga catttccctg caaataggtc agacggctgg
    16141 tgcccttaac atggattttg ccgccgttgg agacattaaa atagccgcga tcgtgtctca
    16201 tgcctgagtt tgactcatac aaatcatcag catataactc gctgccagca ttggagacat
    16261 tcaggaaccc cg
//
LOCUS       DAZPJP010000081        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPJP010000081 DAZPJP010000000
VERSION     DAZPJP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.081"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHU9J_002378"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHU9J_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1905894.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHU9J_002379"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHU9J_002379"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1905895.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHU9J_002380"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHU9J_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1905896.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHU9J_002381"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHU9J_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1905897.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHU9J_002382"
     CDS             6397..6507
                     /locus_tag="ACHU9J_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1905898.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHU9J_002383"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHU9J_002383"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1905899.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHU9J_002384"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHU9J_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1905900.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHU9J_002385"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHU9J_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHU9J_002386"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHU9J_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1905901.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHU9J_002387"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHU9J_002387"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1905902.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHU9J_002388"
     CDS             13514..14446
                     /locus_tag="ACHU9J_002388"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1905903.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHU9J_002389"
     CDS             complement(14487..15917)
                     /locus_tag="ACHU9J_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1905904.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3804 a         4097 c         3941 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcaattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZPJP010000082        15969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPJP010000082 DAZPJP010000000
VERSION     DAZPJP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.082"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(52..204)
                     /locus_tag="ACHU9J_002390"
     CDS             complement(52..204)
                     /locus_tag="ACHU9J_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1905905.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(469..1203)
                     /gene="cysH"
                     /locus_tag="ACHU9J_002391"
     CDS             complement(469..1203)
                     /gene="cysH"
                     /locus_tag="ACHU9J_002391"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1905906.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1278..2990)
                     /gene="cysI"
                     /locus_tag="ACHU9J_002392"
     CDS             complement(1278..2990)
                     /gene="cysI"
                     /locus_tag="ACHU9J_002392"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1905907.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2990..4789)
                     /gene="cysJ"
                     /locus_tag="ACHU9J_002393"
     CDS             complement(2990..4789)
                     /gene="cysJ"
                     /locus_tag="ACHU9J_002393"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1905908.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5108..5470
                     /gene="queD"
                     /locus_tag="ACHU9J_002394"
     CDS             5108..5470
                     /gene="queD"
                     /locus_tag="ACHU9J_002394"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1905909.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5548..6819
                     /locus_tag="ACHU9J_002395"
     CDS             5548..6819
                     /locus_tag="ACHU9J_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1905910.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6810..7070
                     /locus_tag="ACHU9J_002396"
     CDS             6810..7070
                     /locus_tag="ACHU9J_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1905911.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7087..7662
                     /locus_tag="ACHU9J_002397"
     CDS             7087..7662
                     /locus_tag="ACHU9J_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1905912.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7810..8670)
                     /locus_tag="ACHU9J_002398"
     CDS             complement(7810..8670)
                     /locus_tag="ACHU9J_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1905913.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8667..9446)
                     /locus_tag="ACHU9J_002399"
     CDS             complement(8667..9446)
                     /locus_tag="ACHU9J_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1905914.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9424..10374)
                     /locus_tag="ACHU9J_002400"
                     /pseudo
     CDS             complement(9424..10374)
                     /locus_tag="ACHU9J_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10361..11794)
                     /locus_tag="ACHU9J_002401"
     CDS             complement(10361..11794)
                     /locus_tag="ACHU9J_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1905915.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11864..12680)
                     /locus_tag="ACHU9J_002402"
                     /pseudo
     CDS             complement(11864..12680)
                     /locus_tag="ACHU9J_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            12998..14275
                     /locus_tag="ACHU9J_002403"
     CDS             12998..14275
                     /locus_tag="ACHU9J_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR1905916.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14302..15788
                     /locus_tag="ACHU9J_002404"
                     /pseudo
     CDS             14302..15788
                     /locus_tag="ACHU9J_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3748 a         4218 c         4029 g         3974 t
ORIGIN      
        1 gagaccaaac catcacaatt cagtacgcgg ggttttaccc ccgcgtggtg gctacttctt
       61 cgattcgtaa gcgagaactg ccttaaactc cataccacgt tctctgatac tcaactcaca
      121 caacgagtcg cctaccatca gcgtgaatcc cagtactgta aaacacagca ctatgattgc
      181 cacaagggca tattttgtca gcatgtcttg cctccctgca aagaagagac taacattcga
      241 attgtttggg ttcgaacgct ggcctcaggt tgatagaaat atcgcctggg gcttttgtcc
      301 atctggaacc tcgcgaatgc ttaacgccag acagcctcaa gcacccgacg ccattctata
      361 cctgataatt cttccagcgg cgttcacgtt taaaatcagg aatactgctc gcaaaataaa
      421 aacgccccca tcatttctga cagaggcgtt taatttatcc ggcaatattt acccttcgtg
      481 caatccacat tcccttttta agccaaagaa acgtgtttct tcttccgaca tgccaggttc
      541 ccatttacgg gttgtatggg tatcaccgac cgataaatat ccttcatccc ataatgggtg
      601 atatttcagg ccatgttttt gcaggtactg ataaatagtt cggttatccc agtcgataat
      661 cggcagcact ttaaatacgc cacgctgaat tgccagcacc ggtaaattgg cgcgactgcc
      721 ggattgttca cggcgcaggc cagcaaacca ggtttgcgcg ttaagttctt tcagagcccg
      781 gttcatcggt tcgactttgt tgatgtcatt gtacttttca atgccttcaa cgccctgctc
      841 ccacagtttt ccgtagcgtg cttcctgcca ggctgcgctt tcggtagcac ggtacacttt
      901 caggttgagc ttgagtttgt ccgttaactc gtcaataaag cggtaggttt ccgggaacaa
      961 gtaacccgta tcggtgagga tcaccggaat atccgggcga atttgattca ccagatgcag
     1021 gctcaccgcc gcctgaatgc caaagctgga agaaagtaca tattcaccgg gcagattatc
     1081 cagcgcccag gctacgcggc cttcagcgtc cagtttttcc agttcggcgt tagtttccgc
     1141 cagcgccaga atacgatcta ccttcggcag ttcgttcagg gcgtttagat cgagtttgga
     1201 catagattcc tcactgtttg ccttgcctga tgcgacgctc acgcgttctt atcaggccta
     1261 caagaccggg ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg
     1321 atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag
     1381 cgccctatca gttcatcaag cgacgccagg atttccggct cggtgatgtt ttctttatac
     1441 atccgtggga tacgtgtccc aatgcggttg ccgccaagat gcaggttgta gcgacccggc
     1501 gctttaccca ccaggcccac ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct
     1561 gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa attatcgatg
     1621 ttgtcgataa aagacggcag gaaacgctct gcttccgcca tcgccagcgg gcaagtcggg
     1681 aatgacacgc aggccatcga gttttcacgc tgcggcgtga cggcattcat taacccgctc
     1741 tctttggcga tcttctcgat cttcgctttc tcgctttccg gtacaccggt gatgatcaga
     1801 ttctggttcg ccgtaatgcg gaaatcgcct ttgtggatct tcgcgatctc cagcaggccg
     1861 gttttcagcg gacgccccgg ataatcaagg atgcgaccat tttcgataaa cagcgtcagg
     1921 tgccagttat catcaatgcc cttaacccag ccaatacgat cgcctcgtcc ggtgaactca
     1981 tatggacgga tcggttcaaa tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc
     2041 tcaaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc ggttcggtta
     2101 ccccagtcac gctgagttgt cacgacggct tcggccaccg ccagcgtatg ctccagcggc
     2161 agatagccaa actcgctcgc cgtgcgggcg taggttttct tgttgccgtg ttcgatagaa
     2221 agcccaccgc ccaccagcag gttgaagcct accagcttgc cgttttcggc gattgccacg
     2281 aagttcatgt cgttggcatg cagatcgata tcgttctgcg gcgggatcac taccgtggtt
     2341 ttgaacttac gcggcaggta ggtctgacca aggatcggtt cttcatcagt agtggcgact
     2401 ttttcctggt cgagccagat ctccgcatac gcgcgggtac gaggcaacag atgctcagaa
     2461 atcttcttcg cccactcgta cgcttccgcg tgcagctgcg actcgtaagg gttcgaggtg
     2521 cagagtacgt tacggttcat gtcgttagct gtcgccagcg catcaagacc gaccgagtgc
     2581 agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg
     2641 cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt gtcgatcgcc
     2701 tgccactgtt tagtggtaat caccccaccc ggcagacgac agcgaagcag catcgcgtgg
     2761 cgcggctcca gcttctgttc agcacgttcg gcacggatgt cgcggtcatc ctgctgatac
     2821 atgccgtgga agcggatcag caggaagttg tcgcccttaa agccgccggt cagaccgtcg
     2881 tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat gcgctcggcg
     2941 tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat tagtagacat
     3001 ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt
     3061 ccatgccacc aaattcggca atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc
     3121 gattagcgtc gccgcagaca taaatgtggg caccgtcatt gatccagcgc cacagctccg
     3181 cgccctgttc gcgcagtttg tcttgtacat aaactttttc tttttggtcg cgcgaccagg
     3241 caagatcgat acgcgtcagc acgccctctt tgacgtagcg ctgccactcc acctggtaga
     3301 ggaaatcttc cgtaaagtgc gggttgccaa agaacagcca gtttttaccc ggcgcttcgt
     3361 cggcggcgcg ttgctgcata aaggcacgga acggcgcgat gccggtaccg gggccaatca
     3421 taatcactgg cgtttgcggg ttagcgggca ggcggaaatt gtcgttatgt tcgataaata
     3481 cgcggacttc gccctcttct tccacgcgat ccgcgaggaa gctggaggca ccaccggcac
     3541 gggcgcggcc ttccacgtcg taacgcacca caccaacggt gacgtgtact tcgttctcga
     3601 cttccgcctg cgaggaggcg atggaataca ggcgcggggt cagcgggcgc agcaggttaa
     3661 tcagcgcttc ggcatccagt tgcgccggag agaaacgcac catgtcgaca atcggcgtgg
     3721 tcgcggcgta atgctgtaac ttcgctttat cgcccaccag cggcagcagc gtttcgctgc
     3781 gggtaagcgt ggcatagttc tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc
     3841 actgtagcgc ttcgttcaga ggcaacgttt tgccctcgac ggtgacaggt tcatcgcctt
     3901 tcagccacag cagttcgaca agttctttca ccagtgccgg gtcgttctga taccagacac
     3961 ccagcgcgtc acccggctgg taacgcaggc ctgaatcgcc taagtcaatt tcgatatgac
     4021 gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aaccgaaagg ctcgccacca
     4081 gcggcgcgtc tttgctgtac gggctggtgt ggatttcatt taccgcgcca gtagcgacgg
     4141 attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc
     4201 actcgctggc agcagcctgg tattcaacat cggcatcgac acggtcgagc aggcgttcac
     4261 cacccagttc cgccagcttg ctgtcgaaat ctttcccgga ctggcagaaa aattcataag
     4321 agctatcgcc gaggctaaac acggcaaacg cggtgttttc cagctttggc gcttttttgg
     4381 agaacaggaa cttatgcagc gcgacggctt cttccggcgg ttccccttcc ccttgcgttg
     4441 acgtcactac gatgagcagt ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt
     4501 tcaccagctt aacgttcagt tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc
     4561 ggcgcgcatt gccggtttgc gaggcggaga taatagttat acccggcatt tctgcggctg
     4621 gcgctggcgt cgctgcaagc gcagcaggct gctgattgag tacgccccag aaatagccag
     4681 aaacccaggc aagctgggta ggcgttaaat cggtcgtggc cgcctgaagg cgtgccagtt
     4741 gctccgggtt caacggaagc aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg
     4801 ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga agagaagata
     4861 aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa taaccaaatc
     4921 gactaacctg ttttagcaat agtctttaac aacaaaatag attaaccaac ccaatgaaaa
     4981 acaaatgaat ttagccaatc attaagataa atcagcgatt ttgcgcaaca aagtcgtttt
     5041 agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt cctgtagaga
     5101 aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc accgcttacc
     5161 acacgtcccg gaagggcata aatgtggtcg cctgcacggg cattccttta tggtgcgact
     5221 ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg ctgaactgaa
     5281 agcggcgttt aagccaactt atgagcgcct cgatcaccat tatctcaatg atattccagg
     5341 tctggaaaac ccaaccagcg aagttttagc aaaatggatt tgggatcagg ttaaacccgt
     5401 tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgcagggt gtatctatcg
     5461 cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta tgttagggtg
     5521 ttgtgttctg gatatctggg gcatgacatg gaagacgact gcgacattat tattattggt
     5581 gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt atccgttttg
     5641 ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg gttatatacc
     5701 catgcactcg cggaactcct cccgcaattt catctgaccg cgcctcttga acgacgcatc
     5761 actcacgaaa gcctttccct gttaacgccc gatggcgtaa cgacgttttc cagcttacag
     5821 cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct ggttgccgaa
     5881 gccgaaaaag aaggtgtcga atgcatcccc ggagcgacgg tggatgcact gtatgaagaa
     5941 aacggcagag tctgtggcgt tatttgtggt gacgatattc tccgcgcccg ttatgtggtg
     6001 ctggcagaag gtgccaacag cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct
     6061 ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa
     6121 gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt
     6181 gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc gttagggatt
     6241 gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg caagcgagct gctgactcgc
     6301 tttaaagcgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact ggagtatggt
     6361 gcgcatctgg agccagaagg tggcttgcac agtatgccgg tgcaatacgc cggtaacggc
     6421 tggctgctgg tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc
     6481 atggatatgg cgctgactgg cgcacaggcg gcggcacaaa cgctaataag cgcctgccag
     6541 caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca acgtagagcg cagcctgcta
     6601 tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat
     6661 cgggcgtggc ctgcgttaat gcaggatatt tcccgcgatt tatgggatca gggtgataaa
     6721 cctgttccac cgctgcgcca gttattctgg cgtcatttac gtcgtcatgg cctgtggcat
     6781 ctggcgggcg atgttatcag gagtctgcga tgtctgtagc ccataatctc tggcgcgttg
     6841 ctgatgcgcc gcacattgtt ccggctgact ccgttgagcg tcagatggta caacggttga
     6901 ttaacgcctg tccggcaggt cttttttcgc tcacaccgga aggtgactta cgcgttgatt
     6961 atcacggctg cctggagtgt ggcacctgcc gtttgctgtg cgacgaatcc acactacaac
     7021 agtggcgcta tccgccttcc ggattcggca tcacctaccg ctttggataa ataataagga
     7081 taatttatgc ccctcttaca cctactccgc cagaatccgg tgattgctgc cgttaaagat
     7141 aatgccagcc tgcaactggc tatcgattct gaatgccagt ttatttccgt gttgtacggc
     7201 aatatctgca ccatcagtaa tatcgttaaa aagattaaga acgccgggaa atatgctttt
     7261 attcatgttg atttgctgga aggcgcgtca aataaagaag tcgttattca gtttttgaaa
     7321 ctggtgaccg aggcggacgg cattatcagt accaaagcct caatgttgaa agcggcaaga
     7381 gcggaaggtt ttttctgtat tcatcgcctg tttattgttg attcaatttc gtttcacaac
     7441 attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc aggctgtatg
     7501 cccaaagtgc tgggctgggt gacagagaaa atccgccaac cgctgattgc cggtgggctg
     7561 gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc
     7621 acgaataccg gggtctggac gttagcgaaa aaattacttt gacgggataa tcgtaaccaa
     7681 ttgaatttgg tttgatttgt aggccgcacg ccacatccga cattcagcgc ctgatgcgac
     7741 gcttgacgcg tcttatcagg cctacaagtc cccaggccag aaccgtaggc aggatcatcc
     7801 ggcaacacgt tattggcaat ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt
     7861 ccagtcatca accacgccaa catctgcctg tgaaaatacc gcagcgctgg cgtcatgatt
     7921 aatcgccacc acaaacttgc tattgcgtac gcccgccatt aacgctgctg cgccagaagc
     7981 gccaacaaca atacacacct caggtgccag cagatgtccg gaaataccaa tcactttttc
     8041 agcatcaacg ccaccgttca tcactcgcgc ccgactgtag cccacttcgg ctcccagctt
     8101 ttctgccagc atagcgattt cctggttatc tgcctctccg ccctgcccca ccaccagcac
     8161 ccgtctggcc tcagctaatg ggtcgcgagt aacatttttc agatcctctg tgctaaccag
     8221 ccagtccgga agcgcacccg ggacaatgtt cagttgttgc attccagagg gcaacgtggc
     8281 gtttttcgct gctcccgcct gacgcgccag ggaaaggcat agcgggcgtt tttctgtttg
     8341 cagcgtcgcc gttagcgcat ttccccagtg ggatttgcgc acgcttacgg ttggaatatc
     8401 taacgaagtc acctggcaga tgcttgcacc atgtaaacgc caggccagcc gggtcgacag
     8461 ttcatcgcca aacgttcccg gcggaaagag aacaacgtcc gcaggcgttc gctgccactg
     8521 ttccaccaac gcatccagca cctgttccgc cactacaggc tgaggttcaa tctgccagtg
     8581 cgccagggta caaccgctga aatcctgcgc cgccagccag cttgctattg cggcattctc
     8641 ctgatttatt gtcacaattg cgatattcat ggctgcatcc tttggcgtaa atagtcctgc
     8701 catagctttt gagctttttc tgctaccgtt tgcccatcaa tcagcgtcgc tccgcgtcgt
     8761 tgctctgctc ttgccagttg cagacactgc atcgctggca tttcggcagc aacagtttta
     8821 cggataattt ctgctttccc cgcagccatt cgctggcgca ttcccggtac tggcaaggcc
     8881 acttcaccgc actgacgcac ggcaatcacc gcaggcaagc gaacccggca acaacgcaac
     8941 ccatgttcag tacgctgttc gagggtgata aacagcgcgt cgagagtgaa acgctccacc
     9001 tgggtgaagc agggccagcc cagcatttcc gccagcaaaa atggcgtttg cccattctgc
     9061 ccttcgctgc tttggcagcc ggtaattatc agatccagcg gattctgatg ctgccattcg
     9121 gcaatatggc gagcgacaaa ttccggcgca aagcgcagat ctgccgccgt ctccagcaat
     9181 acagcttcct caaacccaag agccatgaga tagcgcagcc agtgcaatgc ccgttcatcc
     9241 cccatgctca acgcggttaa agacatcggc gtaccgtttt ttctctgcgc caacagcagc
     9301 gcggcagcgg cctgttcatc agcaccgagt aaacttcgca gtagcgaaat atccggtccg
     9361 cttttaccct gagccgccgc ctgccactct ttttccgcca gcattccggc atccggttcg
     9421 gctttaaacg ctaacagaat gttcattcgc acctcctaca tttcccgccg ccaccagcgg
     9481 gagggcttta gtttctggtg cccatagcca ggtcacaaca aaatcaacca acaacactgt
     9541 cgccaggagc aataaggtga cttgcattcc ccactgcgcc agcacccacg gcagcaggcc
     9601 agtacttacc gccgcgccaa gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg
     9661 aatgtcagta ggaaaacttt ccgcaggcaa aatgcccacc agattactga ctgccgaaat
     9721 ggtggtgctg aagagaacaa aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc
     9781 catcactacc agcgttgccg ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc
     9841 cagcaggtgc gtcagaacta atcccagcag cgcgcccaca attaacaacg cattaagcat
     9901 caggctggca gtcagcgcat cttccagacc aatagtctgg gcgatagttg gcagccaggt
     9961 ataaatcaca aaccatggga ttacgaggca gacaaagaag acgctgttaa acgccgtgcg
    10021 ccgccagtaa cgcgaagaga acaaggtttt gatgtgttta tgggtcgccg ttaccacttc
    10081 atcgcccagt aaaacatggg gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa
    10141 acgcccctgg cgcagtagcc agcgtggtga ttctggcgtt ccccagcgta ataacgtaat
    10201 caacaacgcg ggcagagctg ccgatgccag tagccagcgc caggcctccg ggttttcgga
    10261 aataaagtga tgtccggcaa tacttgccag cacatagcct acggtccaca ccacgctgaa
    10321 tgcgcccagc aaaataccgc gatggcggcg cggggaaaat tcagccagca aggttaatgc
    10381 cattaggatc gaactgcttt ttcagccctt ccagcaacgc ccacgcgctg ccgtgttcca
    10441 gtttgctcca gtgaacgcga tgtttaccga taccgtggtg gtgcaccatc gaaccgccga
    10501 ggcgaatggt ttcttcacag atgatcttgt tgagcggatt gtggtacttg tcgatttcct
    10561 cttccggctt acagtcaacg acgttgtaat cgtagacgaa gtacatgttg gtgccgttct
    10621 gatagctatg agaggaatga ccgcccagca tggtgatgtc gtcggcgtgc gggaactcag
    10681 tacgaatacg gttaataacg ctttcgtaga tttcgtggat gcagctccag cagccggaca
    10741 cttcagtggt aaagcccatg ttgccggttt tgaggatctg cacacgttcg gcagccactt
    10801 tatccggtcc ccagttcagg ttgttaaacc aggtttcgat cagcttgctg tccacgcgct
    10861 ggcattgcgg gtagcgggca acgatttccg caatcccttc gcccgtcgcc ttcgcaatgc
    10921 gagggttacc ttcagccata aagatcagca cgcattttcc gtctgcaaaa tgggtgaagt
    10981 gttgggtgcc atcttcagcg tcatacaaac gagcgatcga cggacgatac ccttccacca
    11041 tgacttcacg caggatgttg aagtcggttt tcatgtcttc caggatatag ccgtagaaga
    11101 ggttgttttc cggggtgaat ttaaagattt tcactgttac ttcagtgata tagcaaagtg
    11161 caccttcgtt accgatgatg atgtggcgaa tgtccgggcc agccgcgcgg cgtggcacgt
    11221 ttttaatgcg tgtgacggtg ccatcagcca atactgcttc cagaccaacg accatatctt
    11281 cgattgcgcc gtagagtgtg gagaactgcc cgatactgcg ggttgctacc aagccgccca
    11341 tctgcgccag cggctttgac tgcggagaat gccccgtggt gtaacctttt tcacgcaacg
    11401 cattttccag cacctccagc ggaacgccac attgcgccgt cgcctgcata ttctcaatat
    11461 caatattaat gatttgattc atggcggagc cgtcaagcac caccgagttt tccacaacgg
    11521 tttccagccc accttcagtg gcggaagcgc cggtacgcgg cacaccgtta attttgtgcg
    11581 cattcataaa attcagcaca cgagatactt gctctgtgga accgagtttt acgaccgctg
    11641 ccggaatcgg caaagtataa atgccatgaa tatccggaaa tttacgaaaa cggtcaatac
    11701 tgttcttctt taatactgtt tcatcggtaa ttacgcgatc tgcaccaaca atttccttta
    11761 gttggtcgac aatcgctgcg cgagataaag acatagtaat tccttcctga taataaaaat
    11821 ggaataatca ttaataagag aaaacagaaa ccaccgttat ggattagcgc actaaataac
    11881 caccattgac cactaataaa tgcccattga cataatttga agccggactt gcgaggaata
    11941 cagctgcgcc cattaaatcc tgagtatcgc cccaacggtt tgccggaata tgatcaagaa
    12001 cgcgctgatt ggtttctgga ttactgcgtg tcgccagcgt aatatctgtt gcataatagc
    12061 caggagcaat accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg
    12121 tgaatccggc aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt
    12181 aagagaacaa tgaacagata ttaataattt tgccgctttt ctgcgggatc ataatttttg
    12241 cagcttcata gcttaactcg aatgcggcgg tcaggttcac atcaatcatc ggatcccagt
    12301 cggcacgacc gaagtccagc accttattca gcttacaaat accggcattg tttaccagaa
    12361 tatcaactgt accgaaacgc tcacagcaag cagcgataat cttctgcggc gcgccttctg
    12421 cggtgatatc cacctgcatg aagtccacct caacaccctg tttttcaaac atttcctttg
    12481 tttcgccgtt atctttcaat catttccttt gtttcgccgt tatctttgac gaaactagga
    12541 ataaagatat ttgcgccagc tttggccaac gccatggcaa atgcctggcc taaaccgcta
    12601 ttcccaccgg taacaattgc ggttttacct ttcagtgaga aaaaatccat tgagaacgca
    12661 ttgagagatt cgattgacat agttagctcc aatttccgta aggcaaaaaa aagaaaggta
    12721 acttcccccg cgagagggaa gttacctttc tcattatctc tggtaactgg agataatttt
    12781 tagcataacg gcataactgc taatgtgatc gtaatcacag tgtgatattc gtattgtcaa
    12841 atagttttaa aaagcagaag cgtgattact ctcacacaac acctctgctt tccatgttat
    12901 ttcttattgc aattactaga gacctgagaa aagtaatcca cctgaaaggg cggcttactt
    12961 ttctcttttt tttgtctatt acaaatggga atacgatatg caacacaact catatcgccg
    13021 ttggataacc ctcgcgataa ttagttttag cggcggcgtt agttttgacc tggcttattt
    13081 acgttatatt tatcaaattc ccatggcgaa atttatggga ttcagcaata ccgagatagg
    13141 tttaataatg agtacctttg gtattgcggc tattattctt tatgccccca gcggcgttat
    13201 tgccgataaa ttttcacacc gcaaaatgat tacttccgcg atgatcatta ccggattact
    13261 gggtctgtta atggcaacgt atccaccgct gtgggtaatg ctctgtattc aggtcgcctt
    13321 tgcgataacg acgattttaa tgctgtggtc ggtgtcgatt aaagccgcct cgttgctggg
    13381 cgatcatagt gagcaaggga aaattatggg ctggatggaa gggctgcgcg gcgtcggtgt
    13441 aatgtcgctg gcggtgttta ccatgtgggt cttttctcgc tttgcaccgg atgacagcgc
    13501 cagcctgaaa acagtcatta tcatctacag tgtggtttac atcttgttgg ggattctgtg
    13561 ctggtttttt gttagcgata acaacaacct gcgcagtgcc aataacgaag aaaaacagtc
    13621 attccagctt agcgacatcc ttgccgtttt gcgtatcagc accacctggt attgcagcat
    13681 ggtgattttt ggtgtcttca caatctacgc cattctgagt tactccacca actatctgac
    13741 cgaaatgtac ggcatgtcgc tggtggcagc gagctacatg gggattgtga tcaacaaaat
    13801 cttccgcgcg ctgtgcggcc cacttggagg cattatcacc acctacagta aagtgaaatc
    13861 ccctacccgc gtgattcaaa tcctttccat catcggtttg ctggcgttga cagccctgct
    13921 cgtcacgaat tctaaccccc aatcggtcgc gatggggatt ggcctgattt tactgctggg
    13981 attcacctgt tacgcctcac gcgggctgta ctgggcctgc cccggcgaag cgagaacacc
    14041 gtcttacatt atgggcacca cggtaggtat ttgttcggtg attggattcc tgccggatgt
    14101 cttcgtttac ccgattatcg gccactggca agacaccctg cccgctgcag aagcctaccg
    14161 caatatgtgg ctgatgggca tggcggcgct cggcatggtg attgtcttta cctttttgct
    14221 gttccaaaaa attcgtactg ctgatagcgc ccccgcaatg gctagcagca agtaagccag
    14281 gaaaattaca ggagaaaaat catgtcgaaa aaatacatca tagggattga tggcggaagt
    14341 cagagcacaa aagtggtgat gtacgatctg gaaggtaatg tggtttgcga aggtaaaggc
    14401 ttattacagc cgatgcacac gcctgatgcc gatactgcag aacatcctga cgacgattta
    14461 tgggcatcat tatgttttgc aggtcacgat ttgatgagcc agtttgccgg gaataaagaa
    14521 gatattgttg gcatcggcct gggctccatc cgttgctgcc gtgcgttatt gaaagccgac
    14581 ggcacgccag ctgcgccgtt gattagctgg caggatgcac gcgttacacg cccttacgaa
    14641 catacgaatc ctgacgtggc gtatgtaacc tctttttcgg gttatctgac gcatcgctta
    14701 accggcgaat ttaaagataa tatcgccaac tattttggtc agtggccggt ggattataag
    14761 acctgggcat ggagcgaaga tgccgcagtc atggagaagt ttaatatccc ccgtcatatg
    14821 ctgtttgatg tgcaaatgcc aggcactgtt cttgggcata tcacacctca agccgcgctg
    14881 gcgacgcatt tcccggcagg actgccggtt gtttgtacca ccagtgataa accggtagaa
    14941 gctctgggag ccggattact ggatgatgaa actgcggtaa tttctcttgg cacttacatc
    15001 gcattgatga tgaacggcaa agccttgccg aaagatccgg tggcgtactg gccgattatg
    15061 tcttctattc cgcaaacatt gctgtatgaa ggttacggta ttcgcaaagg catgtggacg
    15121 gtgagctggc tgcgcgacat gttaggcgag tcgttaattc aggatgccag ggcgcaggat
    15181 ctttcaccgg aagatttact caacaagaaa gcctctagtg tgccacctgg ctgtaatggg
    15241 ctgatgacgg tgctggactg gctgaccaat ccgtgggaac cgtacaaacg cgggattatg
    15301 atcggctttg attccagcat ggattacgca tggatatatc gttcgatatt ggaaagcgtg
    15361 gcgctgacgc tgaagaacaa ttacgacaat atgtgtaatg aaatgaatca ctttgcgaaa
    15421 catgtaatca ttactggcgg cggttcgaac agcgatctgt ttatgcagat ttttgccgac
    15481 gtgttcaacc ttccggcacg ccgtaacgcc attaacggtt gtgcaagcct gggggcagcg
    15541 attaatacag gggtaggtct ggggctatac ccggattacg caacggctgt cgataaaatg
    15601 gttcgcgtga aagatatctt tataccgatt gagagcaatg ccaaacgcta cgacgcgatg
    15661 aataaaggca ttttcaaaga cctaaccaaa catactgatg tgatcctgaa aaaatcgtat
    15721 gaagtgatgc atggggaatt ggggaattgg ggaatgcgga ttcgatccag agctggtcga
    15781 atgcgtgaat aaggtaggcc gggcggaaaa ctgcccggcc tgaacatacc tgaatggtta
    15841 tccccgctgg cgcggggaac ataagttcga caacttaaaa aatgataaca agtccggttt
    15901 atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta tccctgtttt
    15961 tacaaggaa
//
LOCUS       DAZPJP010000083        15964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPJP010000083 DAZPJP010000000
VERSION     DAZPJP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.083"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHU9J_002405"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHU9J_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR1905917.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLI
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHU9J_002406"
     CDS             complement(1899..2636)
                     /locus_tag="ACHU9J_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905918.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILEKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVLCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQGENITWSLSSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHU9J_002407"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHU9J_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR1905919.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3602..4099)
                     /locus_tag="ACHU9J_002408"
     CDS             complement(3602..4099)
                     /locus_tag="ACHU9J_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HFR1905920.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLVGWGKAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKNQYDGSEKLDFGGIEQPPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            complement(4118..6937)
                     /gene="traG"
                     /locus_tag="ACHU9J_002409"
     CDS             complement(4118..6937)
                     /gene="traG"
                     /locus_tag="ACHU9J_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR1905921.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASNDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSV
                     QERMPGADSPQELMKRAKEYQDKYK"
     gene            complement(6934..8307)
                     /gene="traH"
                     /locus_tag="ACHU9J_002410"
     CDS             complement(6934..8307)
                     /gene="traH"
                     /locus_tag="ACHU9J_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR1905922.1"
                     /translation="MLRIKPLFILCAALLTAIPTASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKSVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8294..8686)
                     /gene="trbF"
                     /locus_tag="ACHU9J_002411"
     CDS             complement(8294..8686)
                     /gene="trbF"
                     /locus_tag="ACHU9J_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR1905923.1"
                     /translation="MTENKKNQEFKIRKIKRNIERSCDNAIKYFELFVVFFVAGLLLW
                     HGMHAFFSAGIDSWKADPELNNLIYMWNILMYAIPYTLYALAAGFLVTSFLSPMYELI
                     FGNIMIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8640..8954)
                     /gene="trbJ"
                     /locus_tag="ACHU9J_002412"
     CDS             complement(8640..8954)
                     /gene="trbJ"
                     /locus_tag="ACHU9J_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR1905924.1"
                     /translation="MRNKQVVLLIAGISGVATGIIVSLNIPFIRQGLFYPASPAEIVV
                     SLCLTFSVSVVFFVGAIVGWISVSEIYYSCMACLNESSEISEETYDDRKQKESGIQNQ
                     KN"
     gene            complement(8944..9489)
                     /gene="trbB"
                     /locus_tag="ACHU9J_002413"
     CDS             complement(8944..9489)
                     /gene="trbB"
                     /locus_tag="ACHU9J_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR1905925.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGAK"
     gene            complement(9476..9760)
                     /gene="traQ"
                     /locus_tag="ACHU9J_002414"
     CDS             complement(9476..9760)
                     /gene="traQ"
                     /locus_tag="ACHU9J_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR1905926.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9841..10176
                     /locus_tag="ACHU9J_002415"
     CDS             9841..10176
                     /locus_tag="ACHU9J_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR1905927.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10157..10498)
                     /gene="trbA"
                     /locus_tag="ACHU9J_002416"
     CDS             complement(10157..10498)
                     /gene="trbA"
                     /locus_tag="ACHU9J_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR1905928.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10512..11255)
                     /gene="traF"
                     /locus_tag="ACHU9J_002417"
     CDS             complement(10512..11255)
                     /gene="traF"
                     /locus_tag="ACHU9J_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR1905929.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLVHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11248..11505)
                     /locus_tag="ACHU9J_002418"
     CDS             complement(11248..11505)
                     /locus_tag="ACHU9J_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR1905930.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEML
                     AACFVIFYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11532..13340)
                     /gene="traN"
                     /locus_tag="ACHU9J_002419"
     CDS             complement(11532..13340)
                     /gene="traN"
                     /locus_tag="ACHU9J_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR1905931.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFRRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGKSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGGAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13337..13975)
                     /gene="trbC"
                     /locus_tag="ACHU9J_002420"
     CDS             complement(13337..13975)
                     /gene="trbC"
                     /locus_tag="ACHU9J_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR1905932.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKSDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13984..14976)
                     /gene="traU"
                     /locus_tag="ACHU9J_002421"
     CDS             complement(13984..14976)
                     /gene="traU"
                     /locus_tag="ACHU9J_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR1905933.1"
                     /translation="MKRSLWLLMLFLLAGHVPSASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKIPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(14973..15605)
                     /gene="traW"
                     /locus_tag="ACHU9J_002422"
     CDS             complement(14973..15605)
                     /gene="traW"
                     /locus_tag="ACHU9J_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR1905934.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEESRK"
     gene            complement(15602..>15964)
                     /gene="trbI"
                     /locus_tag="ACHU9J_002423"
     CDS             complement(15602..>15964)
                     /gene="trbI"
                     /locus_tag="ACHU9J_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1905935.1"
                     /translation="DVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNAPVTAAFNM
                     KQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAVVQGAP
                     DITREIQQDIARRMRAEP"
BASE COUNT         3838 a         4369 c         3723 g         4034 t
ORIGIN      
        1 agcctgagtc acatcttctc cggaggcaac ctccggttca aagaggctgg ccatctgacg
       61 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca gagagagttt
      181 gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata ccccgtcacg
      301 taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctcggggaa cggaaaaaag cacgggtgtt
      421 catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 cggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccacgca tccagtcacg gatggtgaag ggatcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag agtatttgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcatagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gtgccacaac
     1501 tgtggccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactggatct catatactgg
     1621 ctgggacttg attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc
     1681 gttcacaaac gtctgccagc ttatttttac ccataaaatc agaccaacga gtatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatacccg cctgcagccc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcagtcg caatacttca gaccagtcac tcccgtgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacacg ggaggtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaca gagaccaggt aatattttcg ccctgatgac catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggagac ttctgccagc
     2281 tttctgggtg tacagagtac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt ttccagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac cggatgtgcc
     3001 ctgacgcagg gcagcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccggca gcaaggccca caccgagtgt ggcaccggca gaactggaat tatatccggt
     3181 gataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt ttgttttcat caaattacct tttttacaaa ctacagtttt
     3601 cttattgcct gtcataataa tcctgccaac gagtcccaaa agtatttccc gctgagtcaa
     3661 agcctccatg catagggtat ccggttgaag ggttatagta ggtcgttggg ggctgttcaa
     3721 tgcccccgaa gtctaacttt tcactcccat catattggtt tttgagttga ttttgacccc
     3781 atttttttat gcttatgcaa ccactaactc gccttaattc gtatagtgta ccaatgataa
     3841 aaaccatcag gcttaataac ggagagagta aggaagatac tggcatcgtt atataccaaa
     3901 cacctttact aaacttaaac tcatccgaca cattccgttt ttttcgtatt tgagagaaca
     3961 accatataaa cagtccctgc catagcacta tgaagaacag cataaaaact gtttcggcct
     4021 tcccccaacc aaccagatag caggcgataa actgtatcac cgttaccaga gcaatatgag
     4081 ctaaacatct cattatcatc tcccaaacac agcattatta cttatactta tcctgatact
     4141 cctttgccct tttcatcagt tcctgtggac tgtcagcccc gggcattctt tcctgaaccg
     4201 acttttcttc attatattta ttattctgag ataatgcctc tgttttatgg tgattctgta
     4261 gttcagaata ctgtcctctt acaatatttt cctcgccacg tatgctgttc tgagtatcac
     4321 ctatattccc tttatattct gtgaccatat tatcaacctg atgttttaca tcattacgga
     4381 tattactgtc ctgcgttctt tgctcaataa tggcctgatg tccctgatgg tcagcaataa
     4441 tatcttgact gccaccaccc gaaggtacgc tctccattcc tttaccaata tcgccacggg
     4501 attcccgcca ggcattatca acaccaggct gtacctgttc ctgcacaaaa gaccaggcca
     4561 tcgcccgacg acgttctgca atctccggtg aactggtatt tgtcagaata gcttctgcat
     4621 cctgtggcgc gtgtttcatt acatactgtg caaactgttg cgacaagtct tcactcatct
     4681 gcccactcat gctttcagta cgggatgcca tttcagcata ttcatggctg cgggtcatat
     4741 tcgtcgtgta ctggtcgtaa ctctgcttcg ctgaattcag ggctgcagac aactgatcca
     4801 cacgggaatc agcattattg tcagtatggc tgccggactc actgaccttg cgactggtaa
     4861 agtaatcgct ggcctcttta aagtcttgtg tagctctggc atcgatatca tgacgagcat
     4921 cattgctggc ccgtgaacca ctgctggcct gatgcgcatc atcatcactc cagtcaatgc
     4981 cagcccgacc accgcctcta acgcctaatc ctccaccaac accaagaatt ttaggtttaa
     5041 ccccccattc agcatgagca tctccataca tcccggcaga agctctcgta cttctggatg
     5101 ccagttcctg agtcgcctgc tcattactga tattatgcgc cttcgcatag ctttccactg
     5161 cactgcgcat acggctggcc atcatggagt cctgtgcgct catcgtgctg tcagcaccac
     5221 ctgtgacaga atcactgctc ccccggttag aaccaaactg actgagcgag ttccaggcac
     5281 tggcaatact gctgctgaac ccatgcaggg cgctttctgc tctgttcgac gcctcccggg
     5341 ccatttcctg ttgtgccgcc gcaatctgac gcgttgcatt gatgccgacc ggtaaccggg
     5401 acatcgcccc gctcgcatcc atcaccatat taccgtcacg ggtctgtgtg gcagttgcgc
     5461 cactgccggt ctggtacatc atctgaccaa acgacgtggt gctgttggtg ctccagctga
     5521 agccgttcac gttttccgtc tgcatgttgc cgtaggagta attaccgtca accacactgc
     5581 ctgctgcact ggccgtcgga ctgatagagg aagaggcgaa gtggctgtac acgctggaaa
     5641 aacctgcgcc aaggcctttt accatcatcc atgacaacgg ggggatcatg gctgaaagat
     5701 acccggcagt agaggccagg tcagagtatt tcagctgtat ctgagagagt tcagacagca
     5761 cgaccggcgc accattcatc ttcgcataga atgtcatggc actgttcaga atggcataca
     5821 acaacggcca ggtctgcagc cacatcaggg caaacacata gcctttcagg acggacagtg
     5881 tcagtttgtt gaagacagct gccagtacca gaagcgggaa aataccaatg gcaattcccg
     5941 tcaggatagt ctgtaccatt ggcaggtttc gcattgtcac atgaccgata gagacatgcg
     6001 ccagacgttg cttctccatc gatgacgtgg tggccagatt caccaggcta gcggtgtcac
     6061 cattacgggc ggcattactg gtgataccct ctttcagggc attcatggtg acgttctggc
     6121 gcataatctg gctggcagac tgactggagc cgtagaaata actgtaactg tcactgacca
     6181 gttgtctgaa taacaggtcc gggtccggtc tgccgccaaa tatctgctgc acataataat
     6241 gccaggtctt gcctcccgtc tttgtatcga gattcagcct gtctttcagc gtgaccgacg
     6301 catctttaca ggttatgaaa ttattattgc tgtcataaac gccccgtagc gggctgggcc
     6361 gggaaaaaat cagcgtgtag gggtcaccgg aggccatcag gtcttccagc gtgtatttgt
     6421 ggttcaggta aatatccccc agcacgcagt tctggacgta gtcctgaaac agattgatga
     6481 tttccggatt ccgggacaga aaatcggtgc ttttcacaat caggttcgcc ccgaacagca
     6541 tgcctgtctt gctgtaggtg gcgctgtcag gctgggtgaa tatcatctcg taactggcca
     6601 ccattgcatg cccgatacgg gtcgtcagtg aaagtggcat cgccagtccg accggcacat
     6661 tatccacccg gtgaacctgg accaggtcac tgttatcaat aatctgcacc gatgtacgga
     6721 cattaaccag caggctgata agcacaaaca cggccaccca gcccagcaaa tccatcacgt
     6781 tgtgccgctg tacccacatc acggccaccg cgagaacaga caatgtgagc gcaatttttt
     6841 cgatggaatc ccacgtccgg gtccccataa aggcggcaat ggcgttcagg ttattgcgta
     6901 accactcacc accggcaatc acatagactt cattcacagc gtgctccctc cgaagtgata
     6961 gttgttctgg taacgactga gcatgcgggc ggaaagctgc tgacgcatgt agctcatctg
     7021 acgatcgaca accagcagcg catcctgctg tacctgcacc tgtgactgaa acgccgcaat
     7081 ctgccgggtg gcatcattca tgttgtcgtt aatatgctcg ataaccgctt cgtcataatt
     7141 tcccgtggcc accatcgccc gggcctgctg tatcagctcc tgaatgtact gcagcaggat
     7201 gtcgtaaccg atatagtccg tcagctggta aatcatgctg ttggaaacac cgagcatctg
     7261 cgggtcaacc agatatttga agacggggat ggtggtactg gaaataaagc ctttttcctt
     7321 gtcatccaga ggcgtgtcac tgaccgactt gttctgaatg ctggccagaa gtttagtaat
     7381 ctgagacttc agtgcattat cccggctgat ggtcaccggt gtgtctgcca ccactttcag
     7441 gcatttatca gaatcgttgc agtgataaat ttttgctgtg ccgccttcca tcatggcccg
     7501 gataattgag cggtcagtgg ttctggctga caagggtgtg atttcgccgt taggaccaaa
     7561 caccagtgag ccggtcagcg tcatcacaaa ctctttcagc tctttgttgc cgtcaaacat
     7621 tctgtttttg gaaagcgcat tccacatgat gttgatgttt ttggtcaccc gctccttatc
     7681 cttgtcgctg gctttatccc tgacactgtc agatttcccg ccaacggtac atccctgccg
     7741 ggaagccgcc cagtcagcaa aaatattgct ctcaccggca atgtcctgac agactttctg
     7801 ctgggacacc tgcgtccggg ggaagagccc gccgataatc ccctgtgccg cctggcagga
     7861 actgaggttc atactgttaa tgtcgcttgc cattttctgc aggaagtctt ttgcggtttt
     7921 gatttccggc accgttgtct gcagggcaag gtcaaaaaag taaccggcag cgttgctcat
     7981 aatctgctta acaaaacgct gcaactgatc gccattaata aaactgaacg agccgagata
     8041 ggcgtcgatg ccgccacatc cggcattgat atccggcagc gtcatggaaa tcagctggat
     8101 atttttgacc tgggtgcggg catacaggga gccaccgtaa gcataaccgg cggcctgccc
     8161 ctgccagacg cccggctgtg tggtattgga ggcgaagccc agcttgttaa agaactgatt
     8221 catgtcgctg ttcacatccg ctgacgctgt gggtatggcc gtcagtaagg cggcgcaaag
     8281 aataaaaaga ggtttaatgc gaagcattat tgctgccctc ccttaatgtg ttctcccggc
     8341 gcatacggcg tttcagcagg aatatcatga tatttccgaa tattaattcg tacattgggg
     8401 acagaaaaga tgtaacaaga aacccggctg caagcgcata taacgtatac ggaatggcat
     8461 acatcaggat gttccacata taaatcaaat tatttaattc cgggtcagct ttccacgaat
     8521 caataccggc gctgaaaaaa gcatgcatac cgtgccataa aagcaatccg gcaacaaaga
     8581 aaaccacaaa aagctcgaag tattttatgg cgttatcaca actgcgctca atattccgtt
     8641 taatttttct gattttgaat tcctgattct ttttgttttc tgtcatcata agtttcctct
     8701 gatatttctg atgattcatt cagacaggcc atgcagctgt aatatatttc agatactgaa
     8761 atccatccca caattgcccc cacaaaaaac acaacagaaa cagaaaaggt aagacacagc
     8821 gaaacgacaa tttctgcagg gctggcggga taaaaaagtc cctgacggat aaaggggata
     8881 ttcaggctga cgattattcc cgtcgcgacg ccggatattc cggcaatgag taacactacc
     8941 tgcttatttc gcaccttttt cctctccgta catctgcaga acggtatcca tccgcgccat
     9001 aaaactggcg gcatccgttg ccccctgcaa aagcggtaat gcctcaagtg tgttgacgtt
     9061 gaccaggaag gtggtcggtg tggccaccgg aatattcggg aagaaggtct gcatcacgtc
     9121 cggtggcacc ggtaatgctt ccggaaaggc ggtatccccc tgaccatcca gggtgtaggg
     9181 aaaaacggaa aagccgtact gctgtgccag ctgtttcagt accgggtcaa actggtgaca
     9241 gtaagggcaa tgcccctgca taaacagcac caccttccag tcggccaggt taacctgcct
     9301 gccgttgctg aggcggaacc agcggggagc cggcttttct gttctggctg atgtgtctgc
     9361 tgccggttgt gcaggctgca cggccatacc ctgcggattc cagagtcgtt caatttcatc
     9421 ccgggtggag gcctgcacac ctgtggccat catcaacaaa atgaccggca gtaatttagt
     9481 gagagacatg tccgccctcc tgctgccctt ccatcatggc ctgttgcctg gcttccagtg
     9541 cctcacgctc ttccaggctg acacgtgcaa tccatgcatc cagcacctgg tatgcgccga
     9601 acagcacaag gatggccgca atcaactccg ccagaaaaaa agccatccag ggtttgctgt
     9661 aaacaagacg ggcgacgata tgaaaccaga cacccaggga aaatacccac attcccgtaa
     9721 tatcaagccg gggtaaagag aatctgcgtt tacttatcat gttaaactcc ggttattatt
     9781 tacatgagag tattcatgtc tgtaatattc gcgtttgtgt gatattaatc agaggtacaa
     9841 atgaaaaagt tatcttttaa agaaaaactg gaaatagtga gaaacatcat aagagagtca
     9901 ttatcaggta atgcggcttt tgctttactg gtgtattcat tattgcactc cctccccatt
     9961 aaaacctttg ctgatttatt tgtgacgata ctgttattta tagcgattat cgctgttgtg
    10021 atatggctat ttctaatcat ctatgtttat ttttgtgagt tattcagaag tcactggata
    10081 gcagtctggt ttatcctctg gtctgtggta atcaacctca ctattggatt tggcatttat
    10141 gacaaattag caccagtcat ttcactaatg aaataataaa aaaccacatc actccagacc
    10201 atatagctga caacataata caaacaaaca tccttctgaa actgtcaaac ttcgtgataa
    10261 ccggtatggt actgactgcc attgaaacaa ccggtacggc acactgattc ataaccgcac
    10321 cacaaagaaa caagaaatta tctggcagca tacatacact tatgacattt actacaaaga
    10381 agacaaacga gctcagcaga taaactcctt tattatttgg tcctgtaaag aaataaccaa
    10441 caacccccac gataaacagg ctggcacctg taaaaaaaca caaatcccct gtatgcatat
    10501 ataacctttg gttaaaaatt cggtttaaaa tcttcagaaa cgttcaggaa ctgttttgcc
    10561 aggtcgtcct gtgaaataaa gccgtatgat aacgggcgaa cactgccctg tttcgggtca
    10621 accagcatca tggccgggaa atatttcaca ccgaggcgct gcgcctgccc ctggtcagtc
    10681 cgggaatccg gcaacagcgg attaatcacg ccatccacgg aaacgggaat aacactcaga
    10741 ccatacgtat cccggaagcc attaatgacc tgcgccagct gcccgtcgat ggggtcctgc
    10801 ccccggtaaa aaaacatgat gccgtagtgt tcagccagtt tcgcaatggc ctgtcgctgc
    10861 tgcgcctggt ctgccgccag ctgattccgt accgtgccgt tgtaatggct gtactgcagg
    10921 ttatagtcca gttcgggatg taccagcatg gcctttttag cactcatggt gaaaagcccg
    10981 gcctgctgag tccagtaatt ctgcagccgg aaatatttaa caaaattatc cacaccggga
    11041 tacagaatgg cttcgtacag cgcccgcttt gttgccgtct gcagcgcggc cagtttctgc
    11101 ataatatccg gctcctgacg gggggctgca ggtacaggtt tattttcttt ttccttcgga
    11161 tttattttct cgttatacca ctgccagcct gcatctttcc ctgacgccgg aaaaataaag
    11221 cagcagagta acagtggcag taatgcttta ttcatcttca ttcctttcat cattttttct
    11281 ttcatcatct ttctgcaggc cagtcagggc agaaaaacaa aaatacagaa taaacaggag
    11341 cacaccggag taaaaaataa caaaacaagc agcaagcatc tcacctgcag tggtttcttt
    11401 taccgtatcc cacgaccaga taattaccgg ggaaatgaca atcgctgtaa tcaaaagccg
    11461 gataagaaaa tcaataaaac ggttcgacgt gaatatgact ttcatcattt tccccggtta
    11521 ttttccctac cttactgtcc tgcctgtttc agctggtcag caatctgctc tttcactttc
    11581 tgcgtcagaa caccactgtc cgggattttc tggttattca tcaggtcttc gtagaagttg
    11641 gtgaaatcca gtctgtcgaa ctgaattttc tgcagctcat caaccgtaat cccccgacag
    11701 tcaggatgct tcgcaccgcc aaaactgata cgcagctgtc cgttgcgccc ctgctgctgg
    11761 actatctgcg ccagcttcga atcaaactga cagtaactgc gttttttctc caggcagaca
    11821 cccagcactt tcttcgaaca gaactctccg acactgacgg tcagtttgtt tgattttgct
    11881 ttagccaggg ctttttcatc actgctgcat ttggccagcc cgatatcctg tccccagccg
    11941 ctgtccttac agcagttgct gtatccggct gcagccttct tacaaaattt cgcctgaccg
    12001 gtgaaagcac ggacatccac cccgttcagc gccgcaacat ccttacctgc tgcggccagt
    12061 gccgccagct gagaaacggc ttcggcaaaa tcattactct tcccgctctg agccttatca
    12121 cattcgccat cgagacagaa gacgtctccc ccacagacca tcactttccc ggatgttttt
    12181 gactcacagg agtacgtggc atattcatgc agacaggcac cttcttcgga gtaaaaggcg
    12241 cactgatgtg acaccagggt acaggcagga ttgtcggtgt aggtctgaca ggttcccttg
    12301 tctgctgact gtgtgacata cgtgtcccgg tatgcccagc agctctggta catgctgtat
    12361 gactgaccgt ctttcaccag cgtcctgttc ccgccagcct ccgtacattc tgtttttgtc
    12421 agtttcccca gctccttgct gaacgggcag ctttcagacc agacaacata cggggtgtac
    12481 gaggcttttt ttaccctgac aaccagacgc agcttcattg ccgtatgacg tctgaatttt
    12541 gtattcccga cccggacggg gtgagatgac gtaaaagcca caccggcagt cagtgttttc
    12601 tgtactggtg taaatgaggc atctgctgag tatttggcat tccagctcag cggggttccc
    12661 agtactgtaa ttgtcatagg tacactcttc tgcaggtaag tacgactcca gctgtaatgc
    12721 gcatcgacaa tttcaccggt aacgggagag gtaatggaga caacatactg accattcact
    12781 tcacgtgcag gtagctgact catttcatac tccagcgtcc gggtttccca tgtggtactc
    12841 ccctgcagct ccatacgggc agttcgtgtg cagtactgct ccacctgtaa atcccgctca
    12901 caggtgtaat tcgtgtattc actcctgcta atctcctgcg cactgcactg ctgcccggtg
    12961 tttcccacaa tgctgtcagc ccggttcacc acatccctgc cggtctggat aaacggtgca
    13021 tccggtgaaa gaatgtcttt cggcttgttc atgaatgact ctgtaatggt ttttccggtt
    13081 tcaccggtcg cccattccgt ggtgccgtca tttttcaggc cgccatcccc gccggcagtc
    13141 acgccaccgt agtattttgt ctcgtccgga ttcgcgttat agccggggat actctcctgt
    13201 ggcttaaagc cctgaatact gctgcttccc tgtcctttga tctggtgagc aaaatcagag
    13261 ccggcccggt aatcactgtt gctgtcagcc tgtgccatgc cggcaaccag agccagtatc
    13321 agaggtaaaa tacgtttcat ttcccggaat ctcctttccc tgccagtaaa tcatgagcca
    13381 cctgccggca gtcacccgtg gcggccactt tctccagcgc ctggccgacc cgcaggtttc
    13441 cccggatgat gtcgtatccc tgactgcaga acaccaccag tgccggtaca ctgcgaatgc
    13501 cgtactgtga aaacagcgtc gggtcgatct gaacaccatc agtcgcgccg tctttcacca
    13561 gggacaacac agcttcagcg gtggtcttca ggtcattgtt caccatgccc cgcagtgtgg
    13621 cgggtatacc gtagtgccgg gtttcgccca gcattcgttt cagcccctct tcgggaatgg
    13681 aaaacgacac aaaatacagg gcaccctgcc ggggtttccc gtcctgcgaa gcctgctgtt
    13741 tacgaaccag ctcatccagg aaatggttat ctgaacgctg aagggggttt tccagcacct
    13801 gcttctccgc ccaggctttc agctgatggt cagatttttc acgcagttgt ctgcttaaat
    13861 tttcctgctg cttcaggaac tggcggtttt caggagtgtt cacgttttct gacgccataa
    13921 ccgccccgtt cagcatcatc agcagtgctg ccagagattt catactcagc ttcatcattc
    13981 acctcacagg aagacacagt tacgtttacg ccacatcagg tagccgaagt ttttctttgt
    14041 attgggcgga tttttcccgg tttcccagcg ggtcacactg cgcccgaacg ggtggcactg
    14101 tccgctgtcc ggatacatat tcaccatctg ataacgccag cgttctttgg gcaggattgg
    14161 ggacggatat tcattacaga cggcattatt tttcccgatg gtttccataa tcatgccctg
    14221 acggtgcagt ttgaacgcca tgcgttcact gaccagcagg gaggactgca acggactgga
    14281 ctcattactc acccagccat tgaacggata catgctgccc tgtgaaccgg cacaccagaa
    14341 cagaacatcg agaggcatat taaaggcgct ggcaatggca tctgctgcac aggccccctg
    14401 tgctatcgga ttggcaaaga tgacagcttc cggattgaga atggtggtca ggctgctgtc
    14461 cgtccaggtg gggtcaattt cagaaagata agcgatatcc aggtcaccac cttccagaca
    14521 gcccagcgat gtgatgatgt tcagccagta cgtcagcggg tatttgtacc agtgaacgtg
    14581 atagaacgcc ccgttcacct gtttctcatc ctttttcgcc gttccctgtg ccgttttacc
    14641 aaaagccggc aggctgaagc ccaggttaac catgcatccc ggtgaccggg tgacgtccgt
    14701 cagcgccatc ggctcccagt aaccaatggc cagcccgata cgcctgaaca gcggcggcgg
    14761 tgccggacaa atctgaatgg gcatcgacgg gttcgccgtg tcggggatct taccctgact
    14821 gactttgata ctgcccagcg agagcgggaa aatacagctc cagcagatat ccgtgatcgg
    14881 gtttacaaaa cgcccctcac aggcagaatc cgcagacgcc gaaggaacat gaccggcgag
    14941 aaggaataac atcagcagcc acaggcttct cttcattttc tgctctcctc tgccggaata
    15001 aattccacct tcaggaagcg atcgccggga acggcgctca cccgtgccgg gacctgatcg
    15061 atgccgaggc gctggcagag cacaccgttc tggtcaaagt aaacacggct gtccagcgct
    15121 ttctgcatct ccgggatact tccctgcacc aggataattt tgctctccag tgtgggcggc
    15181 gtctggcgtt tcatccaggc cacctgcgcc ggatcatcgc cattgatgaa atacagcgtc
    15241 tggttaaaag gcacatactg cagcggattc atcacttcac cctgacgggc aaacacccgc
    15301 ccttcgttat cccggatatc cgcagccagt ctgacggacg ggtcaaacaa ccggctgctg
    15361 tatttctccg tgcgtccgat accgggcacg gcagggggcc gcagactgtt gcggatcacc
    15421 cgctccttaa aggcatccat tttccggccc atctcaccgg actgctccag tgccgtcagg
    15481 cgctgcatga tcaccgtcag catgtccggc tcctttaccg gccacagatc gccccaggtg
    15541 ccaagatcgg cggcggccac actctgcccc catatcagca gggcaatcag cccccggcat
    15601 ctcatggttc cgccctcatt cgccgggcaa tatcctgctg gatttcccgg gtgatatccg
    15661 gagccccctg taccacggca ggcgacacca gaatgaccgc gtggtgtttc tgctgccacg
    15721 cctgcaggct ggcttccagt gccgtgttaa aacgcgctga aagggctttt gactgtgctt
    15781 ctgacagttg tttctgactg gcgctgtcaa aaaacgcatc cacggtctgt ttcatgttga
    15841 aggcggcagt caccggtgcg ttcagtctga caatgccgta actgacggct gcgttcagta
    15901 acaccattgc caggcatccc ggcaccgtcc accaccagtg gctgcgtcgg ggggctgtaa
    15961 catc
//
LOCUS       DAZPJP010000084        15768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPJP010000084 DAZPJP010000000
VERSION     DAZPJP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.084"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            283..687
                     /gene="sdhC"
                     /locus_tag="ACHU9J_002424"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             283..687
                     /gene="sdhC"
                     /locus_tag="ACHU9J_002424"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1905936.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            681..1028
                     /gene="sdhD"
                     /locus_tag="ACHU9J_002425"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             681..1028
                     /gene="sdhD"
                     /locus_tag="ACHU9J_002425"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1905937.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1028..2794
                     /gene="sdhA"
                     /locus_tag="ACHU9J_002426"
     CDS             1028..2794
                     /gene="sdhA"
                     /locus_tag="ACHU9J_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1905938.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2810..3526
                     /gene="sdhB"
                     /locus_tag="ACHU9J_002427"
     CDS             2810..3526
                     /gene="sdhB"
                     /locus_tag="ACHU9J_002427"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1905939.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3827..6628
                     /gene="sucA"
                     /locus_tag="ACHU9J_002428"
     CDS             3827..6628
                     /gene="sucA"
                     /locus_tag="ACHU9J_002428"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1905940.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6643..7860
                     /gene="odhB"
                     /locus_tag="ACHU9J_002429"
     CDS             6643..7860
                     /gene="odhB"
                     /locus_tag="ACHU9J_002429"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1905941.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7954..9120
                     /gene="sucC"
                     /locus_tag="ACHU9J_002430"
     CDS             7954..9120
                     /gene="sucC"
                     /locus_tag="ACHU9J_002430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1905942.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9120..9989
                     /gene="sucD"
                     /locus_tag="ACHU9J_002431"
     CDS             9120..9989
                     /gene="sucD"
                     /locus_tag="ACHU9J_002431"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1905943.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10093..10815)
                     /gene="mngR"
                     /locus_tag="ACHU9J_002432"
     CDS             complement(10093..10815)
                     /gene="mngR"
                     /locus_tag="ACHU9J_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1905944.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10984..12900
                     /gene="mngA"
                     /locus_tag="ACHU9J_002433"
     CDS             10984..12900
                     /gene="mngA"
                     /locus_tag="ACHU9J_002433"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1905945.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12918..15551
                     /gene="mngB"
                     /locus_tag="ACHU9J_002434"
     CDS             12918..15551
                     /gene="mngB"
                     /locus_tag="ACHU9J_002434"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR1905946.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15576..>15768
                     /locus_tag="ACHU9J_002435"
     CDS             15576..>15768
                     /locus_tag="ACHU9J_002435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905947.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFN"
BASE COUNT         3764 a         3960 c         4352 g         3692 t
ORIGIN      
        1 actgttcagg ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaaca
       61 gcctatactg ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa
      121 cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc
      181 tgtctgaccc gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt
      241 taacctctgt gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg
      301 ataagaaatg tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc
      361 atcacggcga tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg
      421 ggcatcctgc tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct
      481 tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg
      541 gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa
      601 acattcgaag cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca
      661 cttctcgcag gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac
      721 atgatttcat cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg
      781 tcggtttttt cgccaccagt ggcgaactga catatgaagt ctggatcggt ttcttcgcct
      841 ctgcgttcac caaagtgttc accctgctgg cgctgttttc tatcttgatt catgcctgga
      901 tcggcatgtg gcaggtgttg accgactacg ttaaaccgct ggcattgcgc ctgatgctgc
      961 aactggtgat tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg
     1021 gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc
     1081 aggtatgcgc gcggcgctgc aaatttccca gagcggccag acctgtgcgc tgctctctaa
     1141 agtcttcccg acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg
     1201 taatacccat gaagataact gggaatggca tatgtacgac accgtaaaag ggtcggacta
     1261 tatcggtgac caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga
     1321 actcgaacac atgggcctgc cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc
     1381 gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg gcacgcactg cggcggcggc
     1441 tgaccgtacc ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac
     1501 caccattttc tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt
     1561 gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac
     1621 cgtgctggcg actggcgggg cagggcgtat ttatcagtcc accactaacg cccacattaa
     1681 cactggcgac ggtgtcggca tggctatccg cgcaggcgta ccggtgcagg atatggaaat
     1741 gtggcaattc cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg
     1801 tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc
     1861 gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg
     1921 tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac tcgatcacct
     1981 gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg gagctttccc gtacctttgc
     2041 tcacgtcgat ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg
     2101 cggtattccg accaaagtga ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt
     2161 ggttgttccg gggctgtttg ccgttggtga aatcgcttgt gtatcggtac acggcgctaa
     2221 ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca
     2281 tctgcaagag tctatcgccg aacagggcgc attgcgcgat gccagcgagt ctgatgttga
     2341 agcatctctg gatcgtctga accgctggaa caataatcgt aacggtgaag atccggtggc
     2401 gattcgtaaa gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga
     2461 tgcgatggcg aaagggcttg agcagttgaa agtgatccgc gagcgtctga aaaatgcccg
     2521 tctggatgac acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa
     2581 cctgatggaa acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg
     2641 cgcacatagc cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct
     2701 gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg
     2761 cccggcattc ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga
     2821 gttttcaatt tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac
     2881 cctggaagcg gaagaaggtc gcgacatgat gctgctggat gcgcttatcc agctgaaaga
     2941 gaaagatccc agcctgtcgt tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg
     3001 tctgaacatg aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca
     3061 gccgggcaag aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt
     3121 ggtagacatg ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa
     3181 tggacaaaat ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga
     3241 cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg
     3301 gaatcccgat aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga
     3361 tagccgtgat accgagactg acagccgcct cgacggtttg agcgatgcat tcagcgtatt
     3421 ccgctgtcac agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg
     3481 cgccatcggc catattaagt cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat
     3541 aagacgcgca agcgtcgcat caggcaacca gtgccggatg cggcgtgaac gccttatccg
     3601 gcctacaagt cattacccgt aggcctgata agcgcagcgc atcaggcgta acaaagaaat
     3661 gcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga
     3721 gccactacat agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta
     3781 gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc
     3841 tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca
     3901 gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt
     3961 ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata
     4021 tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac
     4081 caatgtgaag caggttaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca
     4141 gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc
     4201 gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc
     4261 cagcggcaaa gaaaacatga aactcggcga gctgctggaa gccctcaagc aaacctactg
     4321 tggcccgatt ggtgccgagt atatgcacat taccagcacc gaagaaaaac gctggatcca
     4381 acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag
     4441 cgaactgacc gccgctgaag gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa
     4501 acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca
     4561 cgctggcaac agcggcaccc gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa
     4621 cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa
     4681 acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt
     4741 ccagaccgat ggcggcctgg tgcacctggc gctggcgttt aacccgtctc accttgagat
     4801 tgtaagcccg gtagttatcg gttctgttcg tgcccgtctg gacagacttg atgagccgag
     4861 cagcaacaaa gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt
     4921 ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg
     4981 tatcgttatc aacaaccagg ttggtttcac cacctctaat ccactggatg ctcgttctac
     5041 accgtactgt actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga
     5101 tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa
     5161 acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg aagccgacga
     5221 gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa
     5281 aatctacgct gacaagctgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat
     5341 ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc
     5401 gatgaacatg cactctttca cctggtcgcc gtacctcaac cacgaatggg acgaagagta
     5461 cccgaacaaa gttgagatga agcgcctgca ggagctggcg aaacgcatca gcacggtgcc
     5521 ggaagcagtt gaaatgcagt ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc
     5581 tgccggtgag aaactgttcg actggggcgg tgcggaaaac ctcgcttacg ccacgctggt
     5641 tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca
     5701 ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgt tgcaacacat
     5761 ccataacggg cagggcgcgt tccgcgtctg ggactccgta ctgtctgaag aagcagtgct
     5821 ggcgtttgaa tacggttatg ccaccgcaga accacgcact ctgaccatct gggaagcgca
     5881 gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cttctggcga
     5941 acagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt acgaagggca
     6001 ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgtg ctgagcaaaa
     6061 catgcaggtt tgcgttccgt ctaccccggc acaggtttac cacatgctgc gtcgtcaggc
     6121 gctgcgcggg atgcgtcgtc cgctggtagt gatgtcgccg aaatccctgc tgcgtcatcc
     6181 gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag ccatcggtga
     6241 aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta
     6301 ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat
     6361 cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca
     6421 cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag
     6481 ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgcac
     6541 ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct
     6601 ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt
     6661 ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa
     6721 cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta
     6781 ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca
     6841 acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa
     6901 accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg
     6961 gaagagcaaa acaacgatgc gttaagcccg gcgatccgtc gcctgctggc tgaacacaat
     7021 ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg
     7081 gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg
     7141 gcgcaaccgg ctctggctgc acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag
     7201 cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac
     7261 gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa
     7321 cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg
     7381 aaacgttacc cggaagtgaa tgcttctatc gacggtgatg acgtggttta ccacaactat
     7441 ttcgacgtca gcatggcggt ttccacgccg cgcggcctgg tgacgccggt actgcgtgat
     7501 gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc agttaaaggc
     7561 cgtgacggca agctgacggt tgaagatctg accggtggta acttcaccat caccaacggt
     7621 ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg
     7681 ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg
     7741 atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc
     7801 ttcctggtaa cgatcaaaga gttgctggaa gatccgacgc gtctgctgct ggacgtgtag
     7861 tagtttaagc ttcacctgca ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa
     7921 aagataacga ttactgaagg atggacagaa cacatgaact tacatgaata tcaggcaaaa
     7981 caactttttg cccgctatgg cttaccagca ccggtgggtt atgcctgtac tactccgcgc
     8041 gaagcagaag aagccgcttc aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt
     8101 cacgctggtg gccgcggtaa agcgggcggt gtgaaagttg taaacagcaa agaagacatc
     8161 cgtgcttttg cagaaaactg gctcggcaag cgtctggtaa cgtatcaaac agatgccaat
     8221 ggccaaccgg ttaaccagat tctggttgaa gcagcgaccg atatcgctaa agagctgtat
     8281 ctcggtgccg ttgtagaccg tagttcccgt cgtgtggtct ttatggcctc caccgaaggc
     8341 ggcgtggaaa tcgaaaaagt ggcggaagaa actccgcacc tgatccacaa agttgcgctt
     8401 gatccgctga ctggcccgat gccgtatcag ggacgcgagc tggcgttcaa actgggtctg
     8461 gaaggtaaac tggttcagca gttcaccaaa atcttcatgg gcctggcgac cattttcctg
     8521 gagcgcgacc tggcgttgat cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg
     8581 atttgcctcg acggcaaact gggcgctgac ggcaacgcac tgttccgcca gcctgatctg
     8641 cgcgaaatgc gtgaccagtc gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa
     8701 ctgaactacg ttgcgctgga cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg
     8761 atgggtacga tggacatcgt taaactgcac ggcggcgaac cggctaactt ccttgacgtt
     8821 ggcggcggcg caaccaaaga acgtgtaacc gaagcgttca aaatcatcct ctctgacgac
     8881 aaagtgaaag ccgttctggt taacatcttc ggcggtatcg ttcgttgcga cctgatcgct
     8941 gacggtatca tcggcgcggt agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg
     9001 gaaggtaaca acgccgaact cggcgcgaag aaactggctg acagcggcct gaatattatt
     9061 gcagcaaaag gtctgacgga tgcagctcag caggttgttg ccgcagtgga ggggaaataa
     9121 tgtccatttt aattgataaa aacaccaagg ttatctgcca gggctttact ggtagccagg
     9181 ggactttcca ctcagaacag gccattgcat acggcactaa aatggttggc ggcgtaaccc
     9241 caggtaaagg cggtactacc cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg
     9301 ttgctgctac tggcgctacc gcttctgtta tctacgtacc agcaccgttc tgcaaagact
     9361 ccattctgga agctattgat gcaggcatca aactgattat caccatcact gaaggcatcc
     9421 cgacgctgga tatgctgact gtgaaagtga aactggacga agccggcgtt cgtatgatcg
     9481 gcccgaactg cccgggcgtt atcactccgg gtgaatgcaa aatcggtatc cagccgggtc
     9541 acattcacaa accgggtaaa gtgggtatcg tttcccgttc cggtacactg acctatgaag
     9601 cggttaaaca gaccacggat tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg
     9661 acccgatccc aggctctaac tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga
     9721 ccgaagcgat cgtgatgatc ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt
     9781 acatcaaaga gcacgttacc aagccagttg taggttacat cgctggtgtg actgcgccga
     9841 aaggcaaacg tatgggccac gcgggtgcga tcattgctgg tgggaaaggg actgcggatg
     9901 agaaattcgc tgctctggaa gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg
     9961 gtgaagcact gaaaactgtt ctgaaataaa tatctgtaat aagaaatagc cctcgccgct
    10021 tccctctaca ggaatggcga agggctgtcg gtttcgacat ggttggccat cgtatgatgg
    10081 ccttttttgt gcttatcgcg atgattttcg ctgcgctatc agggtaaatt tatagtcatc
    10141 ggtattaaaa gcgttgcggc tatattcaaa cacccgacca tcaactaaat atccacgcga
    10201 tactttttca agaatcggct ttgtctggct gatattaagc agacggctca tctcttcggt
    10261 tggcatcaga ggaatgattt cctgttcgct acgatcgata accatcttct tcacttcttc
    10321 aataaagtga tatttcgaat tttccatgac ctgccaggtg agatccggga acaacgcaag
    10381 cggcatccag gtttcttcca gcgccattgg cttttgcttg cgatagcgca cgcgcttcac
    10441 atgccacaca cgatcctgcg cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg
    10501 aatcacttca aatatcagga tttcactgtg tgtatcgacg tgacggtccg acagtttttc
    10561 atcaaaactg gttaactgaa aaatatcgta attgacccgc tcttctttga cgtaagtccc
    10621 gctgccctga atgctttcga ggatctgctg ctcgactagc tggcgcaaag cctgacgcac
    10681 cgtaacccgg ctgacgccaa actctgtttg tagcgctgat tcagtgggta acgcatcgcc
    10741 aggtttaagc tcgccacgcg caatttgttc acgaatgcga tcggcaatct gccggtataa
    10801 gggcttgtgt cccattttta gtatctcatt aatacgaatt tagccattat gcccgataaa
    10861 ttcatcctgt aaataataca aatacaatac aaataatttc aatcaagtga aattgatcac
    10921 atgatggtat tgttttatcg ggcacactgg cgcgactata aaaacgatca agtgaggatc
    10981 atgatgaatc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc
    11041 agccgtgaag aggccatcca cgcgttgact caacgtcttg ctgctctggg gaaaatttcc
    11101 agtactgagc aatttctgga agaagtgtat cgccgtgaaa gccttggccc gaccgcctta
    11161 ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg
    11221 gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga agcagttgat
    11281 ttagtggtgc tgctggcgat tccccccaat gaagcgggta ctacgcatat gcaactgctg
    11341 acagcgctga ccacgcgcct tgcggatgat gagattcggg cgcgtataca gtcggcgacg
    11401 acgcctgatg agttgctctc ggcgctggat gacaagggag gcacgcaacc ttctgcctct
    11461 ttttccaacg cgccaactat cgtctgcgta acggcctgtc cggcgggtat tgctcacacc
    11521 tatatggctg cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt
    11581 gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg cggatcaact caatagtgca
    11641 accgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt
    11701 cccgcgcttt cagtgcctgt tgccgagccg attcgccatg cagaagcgtt gatccaacaa
    11761 gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac agcaagatac gcaaccggtg
    11821 aaaagtgtca aaacggagct gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg
    11881 ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg
    11941 ctacaagatc tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc
    12001 gggctgctcg gaattttgat ggtaccggtg ctcgcggcct ataccgccta ttctctggca
    12061 gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat gatcggctcc
    12121 gggtttctcg gcgcggtcgt tggcggattg atagccggtt acttgatgcg ctgggtgaaa
    12181 aatcacttgc gtcttagcag taaattcaat ggattcctga ctttttatct ctacccggtg
    12241 ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg
    12301 atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg
    12361 ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa caaagccgct
    12421 tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc
    12481 tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc accgcgcctg
    12541 tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct ggcaggtatt
    12601 accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg
    12661 ctgggctcta tggtaacggg cgctattgtc ggtgcgatga atatcggcct ttcgacaccc
    12721 ggtgccggca ttttctcgct ctttttactt catgataatg gcgcgggcgg tgttatggcg
    12781 gcaattggct ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc aattctcctg
    12841 atctggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt aatgccataa
    12901 acaaaaagga aacgacgatg aaagcggtat ctcgcgttca catcaccccg catatgcatt
    12961 gggatcggga gtggtatttc accaccgaag agtcacgcat tctgctggtc aataatatgg
    13021 aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta ctcgacgggc
    13081 aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga
    13141 aacaggtaga agccggtaag ttgattatcg gcccctggta tacccagacc gataccacga
    13201 ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt
    13261 ttggcgagcc gatgaaaata ggttatttac cggattcctt tggcatgtcc gggcaactgc
    13321 cgcatattta caatggattt ggcattaccc gcactatgtt ctggcgcgga tgttcggagc
    13381 gccacggtac tgataaaacc gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg
    13441 cgcaggtgct gccgctgggc tacgccatcg gtaagtactt acctgccgac gaaaacggat
    13501 tacgtaaacg cctcgacagt tattttgacg tgctggaaaa agcgtctgta accaaagaga
    13561 ttttgctgcc gaatggtcat gaccagatgc cattgcagca aaatatcttc gaagtgatgg
    13621 ataagctacg tgagatctac cctcaacgta agtttgtgat gagccgcttt gaagaggtat
    13681 ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg
    13741 gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg catggatatc aaaattgccc
    13801 acgcacgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg ctggcctgga
    13861 cgttgggttt tgaataccac cacggcttgc tggagaaaat gtggaaagag atcttaaaaa
    13921 atcatgccca cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg
    13981 cccgcttcga actggctgaa gacatggcgg ataatctgct gcgtttctac atgcgcaaaa
    14041 ttgccgataa catgccacag agtgatgccg acaaactcgt gctgtttaac ctgatgccct
    14101 ggccgcggga agaagttatt aacaccactg tgcggctgcg cgccagccag tttaatctac
    14161 aagacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag atcgatcctg
    14221 gcctaatcga tcggcaaatt gttcattatg gcaattatga tccctttatg gagtttgata
    14281 ttcagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatt gaagcgaatc
    14341 agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gattctggaa aatgctttct
    14401 ggcagattgc gctcaatgag gatggttctc tgcaactggt agataaagac agcggtgtgc
    14461 gctatgaccg ggtattgcaa attgaagaaa gctctgatga tggtgatgaa tatgactatt
    14521 cacccgcaaa agaagagtgg gtaattaccg cagcgaacgc gaaaccgcaa tgcgatatta
    14581 ttcatgaagc ctggcagagc agggctgtta tccgctatga catggcagtg ccgctcaatt
    14641 tgtcagaacg cagcgcccgg caatccactg gcagagtagg gggggtgttg gttgtcactc
    14701 ttagtcataa cagcaggcgt attgatgtgg atatcaacct tgataaccag gctgacgatc
    14761 atcgccttcg tgtcctggtc cctacacctt ttaacactga cagtgttctg gcagatacgc
    14821 agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg cagcaagaag
    14881 gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgttgcc ttacaggaag
    14941 ggcgtaacgg catggctgtc tttagcgaag ggttacgtga atttgaagtc atcggtgaag
    15001 agaagaaaac ctttgccatt acgttgctgc gtggcgtggg cttactgggc aaagaagatc
    15061 tgcttttaag gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac tcacaactac
    15121 gtggtctgct ttcttgtcgc ctaagtttat tgagttatac cggtacgcca accgccgctg
    15181 gtgtagctca gcaggcgcga gcatggctga ctccagtaca gtgttacaac aaaatcccat
    15241 gggatgcgat gaagctcaac aaagccggat tcaacgtgcc ggaaagttat agtttgttga
    15301 aaatgccccc agtgggatgc ctgataagcg cacttaagaa agctgaagac cgacaagaag
    15361 tgattttacg gctgtttaat ccggctgaat cagcaacctg tgatgcgact gttgctttca
    15421 gtcgcgaggt gatttcttgc tcagaaacga tgatggatga acacattacc accgaggaaa
    15481 atcaaggttc aaatctatcg gggccttttt tacccggcca gtcacggacg ttcagttacc
    15541 ggcttgcctg aatagcaatc aaaccgaagc cacatatgcg cggccagatt gttgacaaag
    15601 ggcgctttgt tcatgccgga tacggcatga acgctttatt cggtctacaa aagcaggcaa
    15661 attcaatata ttgcagagat gatgtagaca ctggcaagcg tagcgcatca ggtaattttg
    15721 cgtttatctt cactctcaag ccacgtatat gtggctttat ttttaaca
//
LOCUS       DAZPJP010000085        15580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPJP010000085 DAZPJP010000000
VERSION     DAZPJP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.085"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            46..144
                     /locus_tag="ACHU9J_002436"
     CDS             46..144
                     /locus_tag="ACHU9J_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1905948.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            147..221
                     /gene="yoaJ"
                     /locus_tag="ACHU9J_002437"
     CDS             147..221
                     /gene="yoaJ"
                     /locus_tag="ACHU9J_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1905949.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(280..528)
                     /locus_tag="ACHU9J_002438"
     CDS             complement(280..528)
                     /locus_tag="ACHU9J_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1905950.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(676..858)
                     /locus_tag="ACHU9J_002439"
     CDS             complement(676..858)
                     /locus_tag="ACHU9J_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1905951.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(862..1221)
                     /gene="yeaR"
                     /locus_tag="ACHU9J_002440"
     CDS             complement(862..1221)
                     /gene="yeaR"
                     /locus_tag="ACHU9J_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR1905952.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1394..2032)
                     /gene="leuE"
                     /locus_tag="ACHU9J_002441"
     CDS             complement(1394..2032)
                     /gene="leuE"
                     /locus_tag="ACHU9J_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1905953.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2159..3082)
                     /gene="dmlR"
                     /locus_tag="ACHU9J_002442"
     CDS             complement(2159..3082)
                     /gene="dmlR"
                     /locus_tag="ACHU9J_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1905954.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            3185..4270
                     /gene="dmlA"
                     /locus_tag="ACHU9J_002443"
     CDS             3185..4270
                     /gene="dmlA"
                     /locus_tag="ACHU9J_002443"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1905955.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            4521..6131
                     /gene="yeaV"
                     /locus_tag="ACHU9J_002444"
     CDS             4521..6131
                     /gene="yeaV"
                     /locus_tag="ACHU9J_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1905956.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6163..7287
                     /gene="yeaW"
                     /locus_tag="ACHU9J_002445"
     CDS             6163..7287
                     /gene="yeaW"
                     /locus_tag="ACHU9J_002445"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1905957.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7343..8308
                     /gene="yeaX"
                     /locus_tag="ACHU9J_002446"
     CDS             7343..8308
                     /gene="yeaX"
                     /locus_tag="ACHU9J_002446"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1905958.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8362..9477)
                     /gene="rnd"
                     /locus_tag="ACHU9J_002447"
     CDS             complement(8362..9477)
                     /gene="rnd"
                     /locus_tag="ACHU9J_002447"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1905959.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9559..11244)
                     /gene="fadD"
                     /locus_tag="ACHU9J_002448"
     CDS             complement(9559..11244)
                     /gene="fadD"
                     /locus_tag="ACHU9J_002448"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1905960.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11449..12030)
                     /gene="yeaY"
                     /locus_tag="ACHU9J_002449"
     CDS             complement(11449..12030)
                     /gene="yeaY"
                     /locus_tag="ACHU9J_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1905961.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(12070..12765)
                     /gene="tsaB"
                     /locus_tag="ACHU9J_002450"
     CDS             complement(12070..12765)
                     /gene="tsaB"
                     /locus_tag="ACHU9J_002450"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1905962.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12823..14733)
                     /locus_tag="ACHU9J_002451"
     CDS             complement(12823..14733)
                     /locus_tag="ACHU9J_002451"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1905963.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14865..15209
                     /locus_tag="ACHU9J_002452"
     CDS             14865..15209
                     /locus_tag="ACHU9J_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1905964.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3833 a         4066 c         3787 g         3894 t
ORIGIN      
        1 gccacattgc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt
       61 atttttccgg ttgtaatctt tattacagcg gtcgtatttt tagcatggtt ttttattggc
      121 ggctatgctg ccccgggggc ataaagatga aaaaaacaac gattattatg atgggtgtgg
      181 cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag
      241 caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta
      301 gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa aattgaagcc
      361 atcgacttta ccaaagccaa acagcgtgct gatccatccg ccgactacgg caccgactat
      421 ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga tccacttcgc
      481 cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg tttcctcact
      541 tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac atcgggaggg
      601 caagatttgt gacgagcatc acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc
      661 aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg
      721 cacggtattc accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat
      781 cggcgtctct gtttcataat cgacagaaac gccattactg ttattggtga cggtcacggt
      841 atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcacc
      901 ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat
      961 ggcttcaatg ttgtgccact tttctggagg gaacacctca aactgccccg cttcgataag
     1021 gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc
     1081 atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc
     1141 gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata
     1201 attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta
     1261 ttccagccgt ttcagggtac gcctgataat ttgcatttta aataccattt attggttact
     1321 ttttagcacc atatcagaga aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg
     1381 cgggctgaat gtatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca
     1441 aaccaatcag tgagttgcca actttagcca gtttcttttt ggtacgtatg tactgcgtga
     1501 caaaagcacc agaaataatc aggaagctca aatagcagaa actcaccagt tccagcgtcg
     1561 tcgccagaat aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga
     1621 aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt
     1681 taaaaatggc cccgtattgg ggctcatcgg atttggcctc gttattttta cccttcaggg
     1741 tcgcgtagag aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacaa
     1801 tgttgaataa tatcggggtg gtcttaatta atgtcgccac tccagcccat gccagaaaca
     1861 tcaatactgc atcgccaata aatacaccgc aggccgcaag ataaccgcct ttcataccgc
     1921 tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa
     1981 tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct
     2041 ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg
     2101 cttagaaagg agaaaatcag gttttaaccc gatatcaacc cgataattga ataattaatc
     2161 aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta
     2221 tcgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacgttcg
     2281 ctggctggta atattccggt agcacttgca ctaaatgacc actggcaatg ttttcgctaa
     2341 catcccacca ggagcgcaga gcaatcccct gcccatcgag acaccactga tggacaattt
     2401 ctccatgatt agacgacagt gggccggtga ctttaatcgc atggtggcct tctttattgc
     2461 gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg
     2521 aaagatcggt taaatgtttc ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga
     2581 gaatgcggta attagtcgcc agtttgcggg cgattaaatt aggggcgatg tcatcgccaa
     2641 tgcgaatatc gagatcaaca ccttcattga ccaaatcgac caaccggtct tccacatcaa
     2701 aacgtaattc cagttgcgga tacgccttag ccagcgctga taacgccgga gccaccacct
     2761 gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctgcggcacc tgacgcacgt
     2821 cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa
     2881 tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt
     2941 ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt
     3001 cttccgccac ggcggcaaaa ccagcccggc gagccaccag cataaagacg cgcaaatcgt
     3061 tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac ggggattaat
     3121 tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa
     3181 cccaatgatg aaaacgatgc gtattgctgc gatcccggga gatgggattg gcaaagaagt
     3241 ccttcctgaa gggattcgcg tattacaggc tgccgctgag cgctggggct tcgacttgag
     3301 ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta aaatgatgcc
     3361 ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg ccgtcggctg
     3421 gccggatacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga
     3481 attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct
     3541 ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca ccgaaggcga
     3601 atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg tcattcagga
     3661 atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca
     3721 aagccgccca cgtaagacgc taacttctgc cactaaatca aacggtttag ccatcagcat
     3781 gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga tccgctggga
     3841 caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt
     3901 ggtggtggcg tccaatttgt ttggcgatat cctttccgat cttggcccgg cctgcaccgg
     3961 caccattggc attgccccat ccgccaacct gaatccggaa cgcactttcc cgtcgctctt
     4021 cgagcctgtc cacggttccg cgccggatat ctacgggaaa aatattgcta accctatcgc
     4081 cacaatttgg gccggtgcga tgatgctcga ttttctcggc aatggcgatg agcgtttcca
     4141 gcaagcgcat aacggtattc tggcagcgat tgaagaagtg attgctcacg ggccgaaaac
     4201 accggatatg aaaggcagtg ccaccacgcc acaggttgcc gacgcgattt gcaaaattat
     4261 tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt tttttattcg
     4321 ttctttgcag ttaataacct gcgtcatttc accttttatt gtttccgttt cgtgttttat
     4381 ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa tttgttaaac
     4441 acaacataca actcattacc ctgcaaataa ttacaactgg caaaagccag agacgggctt
     4501 ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa
     4561 gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat
     4621 cggccgacgc ggccaacaca atatacactt ttgttacgcg tacgttaggt tccgccgtac
     4681 aggtattggt tttgctggca atgggactgg tgatttattt agccaccagt aaatacggca
     4741 atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta
     4801 tttgtgccgg tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt
     4861 atcaaacgcc tggattaaat atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc
     4921 cttactcctt cttccactgg ggcatcagca cctgggcaac ttatacgctg gcctcattaa
     4981 tcatggctta tcactttcat gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg
     5041 ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa actggttgat ttgatgtttc
     5101 tgatcgccac tgtcggcgca ctgactattt cccttgttgt caccgcagca acctttactc
     5161 gtggactttc cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc
     5221 tgctttccgg cggcattttt tgcctaagct cgtggattgg tatcaacaac ggtttgcaac
     5281 gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg
     5341 gtccaaccga atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact
     5401 tcctgcaaat gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga
     5461 ccgttttcta ctggctgtgg tggatctcat acacccctgg cgtagcaatg tttgtcaccc
     5521 gcgtttcccg cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg
     5581 tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc cattcatcag tttatcaatg
     5641 gcgtaatcaa cgtcccacag gtgctggaaa cactgggcgg cgaaacagct gtacagcaag
     5701 ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta
     5761 ttttccttgc ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcgta
     5821 atctccagga aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca
     5881 ctctgatccc gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg
     5941 tcgtgctcac agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc
     6001 gctggctgaa acaggattat gccgacattc cggctcatca agttgaacat tatctcccgc
     6061 agacaccggt tgaagccctg gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag
     6121 gcgacaactg agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct
     6181 gactttgtac tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc
     6241 cgtttttata ccgatcagaa cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc
     6301 tggatttgcg tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag
     6361 atcattggcg aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat
     6421 aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg
     6481 attacctgcc catatcacgc atgggcattc aaactcgatg gcaatctggc ccatgcacgt
     6541 aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg
     6601 gaagaatatg ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat
     6661 cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa
     6721 ctggcggccc gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat
     6781 ctcgagtgct atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat
     6841 cgctactggc acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc
     6901 gaacagtcgt ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg
     6961 ccgtgcacga tgctgaacgt caccccgatc aaagggatga tgacggtcat ttatgaattc
     7021 ccggtggatt ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta
     7081 accgacgagc aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta
     7141 cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc
     7201 atcatggccg acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat
     7261 ctgctggcgc aggtgtttaa ggactaatga catcggcggc ggtattttcc gccgctgggc
     7321 tgatttttga tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca
     7381 ggtggaaccg cttaccgaac aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc
     7441 gctgcctgcg ttcaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca
     7501 gtacagcaat gcgtattcac tactgagttc gccgcatgac acctcttgtt atcagattgc
     7561 cgttcggctg gaggaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt
     7621 gggcgatcgg ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcagccag
     7681 aaagcatctg tttatcgcgg gcggtattag tatcacccct ttcctgtcgc acatggcaga
     7741 gttgcaacac agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg
     7801 cgcatttcgt gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc
     7861 atcaaccgga acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca
     7921 cgtttatacc tgtggccccg aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct
     7981 ggacatcgcc gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga
     8041 tgcctttacc ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac
     8101 tattttgcag gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg
     8161 ggtatgcgga acctgcgaaa cagcaatact ggaaggtgaa gctgaccatc gggatcagta
     8221 ttttagcgat gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa
     8281 gggtaaacgc ctggtgttgg atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc
     8341 ccagtccggc ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc
     8401 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
     8461 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
     8521 gatcttatgc gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg
     8581 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt
     8641 ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga
     8701 taaacccagg ctgtccagtt cgcctaaact tcccggcata taacgcgcga ccgaccacaa
     8761 atgctcttca cgcacgacaa agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg
     8821 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt
     8881 gatatcacgc caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
     8941 gcattcatcc agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc
     9001 ggtgatcggt aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag
     9061 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
     9121 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
     9181 cgtgtcaatc aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
     9241 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
     9301 ccagtcggtg atcccgagag gatcaattag cgccagatgc tcaccatcga aaagttgaat
     9361 caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc
     9421 cggaaagaca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
     9481 gtaattcaaa ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
     9541 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
     9601 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
     9661 cttcggtact ttgtagcccg tgagctgacg gcagcaaaag gtcaccagtg actcttcggt
     9721 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
     9781 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
     9841 gttgggatag acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg
     9901 caggaaccct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
     9961 gatctcatcg gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
    10021 acaaagctca cccggttgac ccggtggtac ttcattatcg tcgtcatcca ccagtttggc
    10081 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt
    10141 aacgctgacc agcggcgcac actcggtaag accatagcct tccagcagat actgtcctgt
    10201 cagtttcacc caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag
    10261 atgcagactg gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa
    10321 ggtgttaacg ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg
    10381 aatatcgcgc gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca
    10441 gttaatggtc agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc
    10501 tggatgcagc aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg
    10561 gtgagtcagc atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc
    10621 taaatcttcc ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc
    10681 gctacgaaat gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt
    10741 aacaacgaaa ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag
    10801 aattacgtgc tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga
    10861 cacgataaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    10921 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    10981 cggatattgc aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    11041 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat
    11101 tacctccccc atattcacaa acgcaggctg atcggcgtag cggacgaccg actgctcaaa
    11161 catatctatc agagattgat aacggtcagg gttgatctcc gtcggaacat ccgcgggata
    11221 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    11281 aaccccaagt gataaacatg ttgttaacat aacattaact catcttacca gcttgataat
    11341 tagacaacga aaaggttgcg aagcgcgtca ctatttattt ttatagttgc cgtatgaacg
    11401 cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa acaacttgtt actcggttac
    11461 aattgtttca actcttgcag ggccaggatt ataccaaccc catccgccgt aaccataggg
    11521 ccagccgcgt ccaccataaa accatggatc aattggctgc ggcggcataa tcacctgttg
    11581 agtgatatgc cagcgtttgt aacctgttac ctgcataacc ataaatttat atggtgcatt
    11641 atttactttg ccatccactg tgccagtgat tggcccgaca accgtcacca gttgtccacg
    11701 aaaatcgacc ggatcaagaa aaccgttcac atcggcataa atgcgcccgc gtgaaggttc
    11761 tcccaaggtt ggtcttgctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt
    11821 cccttgctgg ttttgtacca caaccacttt gccgccaaag cgcgcctcct gaccaacata
    11881 taaatctggc gcattcatca cgcgtaccag atcttgctgt ggtgttggac tgctgccttt
    11941 aatggcgtcc ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    12001 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    12061 tcttaagatt cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac
    12121 cggttcggca tgttccaccg ccaccgtttt accctcggca aacatctgac acgcaatcgg
    12181 cagcatatct tccgccgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc
    12241 tttacggaga tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag
    12301 ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg
    12361 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    12421 aatggctgcc agcacgcggg ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat
    12481 tagcgtagag acaccaatca tcggtaactc cgcgccaagc gccagccctt gcgcaatacc
    12541 aataccaatg cgcacgccag taaagctacc ggggccgcgc ccgtaagcca gagcgtttat
    12601 gtcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    12661 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc
    12721 gacagagcag acctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    12781 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    12841 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc
    12901 gagaaacgtc gcgccgtaag gacgcatcac cagccgattg tcacaaatta ccagcacgcc
    12961 gcgatcgtcg gcgtcgcgaa tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc
    13021 cggcagctgc acttcatcga acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc
    13081 ttttaacagt agatcatccg gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa
    13141 tgtatcgcca cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt
    13201 accggcgctg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac
    13261 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg catgcgaggt
    13321 acaaagcata aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag
    13381 ttgccgtgca caacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    13441 ctggcggctg taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag
    13501 ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca
    13561 gctaccgggc ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    13621 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    13681 ctcttttagc cgcttcagcc gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag
    13741 caaggcggaa cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc
    13801 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    13861 ataacctggc tcaccgagtt gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc
    13921 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    13981 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    14041 cggtagctgg tgggcttcgt cgaagatcat gacgtccgct tcagggatca actcgccaaa
    14101 tccactctct ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    14161 ggcgtccatc gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    14221 gctgccaaga cagttgtcgt tggtgctggt gaccagcggc cacgcctgtg aatcttccgc
    14281 cacgctgacg caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag
    14341 gatcacatcg cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag
    14401 acgttcgagg cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt
    14461 caatgccttt gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga
    14521 gccggtcgag ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt
    14581 tttgcccgta ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    14641 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    14701 cagctgaccg tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    14761 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg
    14821 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
    14881 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
    14941 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacg ctggcacaga
    15001 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    15061 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    15121 cgggtcatgc gccagtgcgc tgcacggtac aagcgggttt gatgaacccg aagtataaag
    15181 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    15241 atttatcgat aatactggtc ggttttacat aaatcgacca gagaataaga tattactgtt
    15301 gatgcttctg ctaattcatt atttatattt ataatttcaa ttttatctat aaaactaact
    15361 atataaataa acataaccat gccgttgtgt tcgccctttg attagcaact ctggttttct
    15421 ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc tccttcttac
    15481 tggataacac accatgagct gctaccttta aaacacctga atgtcaacag gttaactcgt
    15541 gccatatcgt tataatccct ttgatattag atgcaaatta
//
LOCUS       DAZPJP010000086        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPJP010000086 DAZPJP010000000
VERSION     DAZPJP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.086"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHU9J_002453"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHU9J_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1905965.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHU9J_002454"
     CDS             567..650
                     /locus_tag="ACHU9J_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905966.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHU9J_002455"
     CDS             750..1571
                     /locus_tag="ACHU9J_002455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1905967.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHU9J_002456"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHU9J_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1905968.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHU9J_002457"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHU9J_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1905969.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHU9J_002458"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHU9J_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1905970.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHU9J_002459"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHU9J_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1905971.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHU9J_002460"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHU9J_002460"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1905972.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHU9J_002461"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHU9J_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1905973.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHU9J_002462"
     CDS             8347..9729
                     /locus_tag="ACHU9J_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1905974.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHU9J_002463"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHU9J_002463"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1905975.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHU9J_002464"
     CDS             complement(10485..10820)
                     /locus_tag="ACHU9J_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1905976.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHU9J_002465"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHU9J_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1905977.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHU9J_002466"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHU9J_002466"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1905978.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHU9J_002467"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHU9J_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1905979.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHU9J_002468"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHU9J_002468"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1905980.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3812 a         4021 c         3793 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPJP010000087        15453 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPJP010000087 DAZPJP010000000
VERSION     DAZPJP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.087"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHU9J_002469"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHU9J_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1905981.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHU9J_002470"
     CDS             1598..4654
                     /locus_tag="ACHU9J_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1905982.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHU9J_002471"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHU9J_002471"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1905983.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHU9J_002472"
     CDS             5161..5349
                     /locus_tag="ACHU9J_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1905984.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHU9J_002473"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHU9J_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1905985.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHU9J_002474"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHU9J_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1905986.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHU9J_002475"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHU9J_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1905987.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHU9J_002476"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHU9J_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1905988.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFASLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHU9J_002477"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHU9J_002477"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1905989.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHU9J_002478"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHU9J_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1905990.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHU9J_002479"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHU9J_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1905991.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHU9J_002480"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHU9J_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1905992.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHU9J_002481"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHU9J_002481"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1905993.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHU9J_002482"
     CDS             complement(14591..14785)
                     /locus_tag="ACHU9J_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1905994.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHU9J_002483"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHU9J_002483"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1905995.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3903 a         3740 c         4092 g         3718 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgc
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gta
//
LOCUS       DAZPJP010000088        15408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPJP010000088 DAZPJP010000000
VERSION     DAZPJP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.088"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHU9J_002484"
     CDS             <1..454
                     /locus_tag="ACHU9J_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1905996.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHU9J_002485"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHU9J_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHU9J_002486"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHU9J_002486"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1905997.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHU9J_002487"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHU9J_002487"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1905998.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHU9J_002488"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHU9J_002488"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1905999.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHU9J_002489"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHU9J_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1906000.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHU9J_002490"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHU9J_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1906001.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHU9J_002491"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHU9J_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1906002.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHU9J_002492"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHU9J_002492"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1906003.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHU9J_002493"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHU9J_002493"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1906004.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHU9J_002494"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHU9J_002494"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1906005.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHU9J_002495"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHU9J_002495"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1906006.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHU9J_002496"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHU9J_002496"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1906007.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHU9J_002497"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHU9J_002497"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1906008.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHU9J_002498"
     CDS             13056..13436
                     /locus_tag="ACHU9J_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1906009.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHU9J_002499"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHU9J_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1906010.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHU9J_002500"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHU9J_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1906011.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15408)
                     /locus_tag="ACHU9J_002501"
     CDS             complement(15245..>15408)
                     /locus_tag="ACHU9J_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1906012.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4045 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat ataacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcagc
//
LOCUS       DAZPJP010000089        15349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPJP010000089 DAZPJP010000000
VERSION     DAZPJP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.089"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHU9J_002502"
     CDS             <1..1015
                     /locus_tag="ACHU9J_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1906013.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHU9J_002503"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHU9J_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1906014.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHU9J_002504"
     CDS             1797..1904
                     /locus_tag="ACHU9J_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1906015.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHU9J_002505"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHU9J_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1906016.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHU9J_002506"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHU9J_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1906017.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHU9J_002507"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHU9J_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1906018.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHU9J_002508"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHU9J_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1906019.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHU9J_002509"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHU9J_002509"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1906020.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHU9J_002510"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHU9J_002510"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1906021.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHU9J_002511"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHU9J_002511"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1906022.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHU9J_002512"
     CDS             complement(11226..11813)
                     /locus_tag="ACHU9J_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1906023.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHU9J_002513"
     CDS             11983..12561
                     /locus_tag="ACHU9J_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1906024.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHU9J_002514"
     CDS             complement(12691..13452)
                     /locus_tag="ACHU9J_002514"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1906025.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHU9J_002515"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHU9J_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR1906026.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHU9J_002516"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHU9J_002516"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1906027.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3746 a         4180 c         3840 g         3583 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgca
//
LOCUS       DAZPJP010000090        15215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPJP010000090 DAZPJP010000000
VERSION     DAZPJP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.090"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..350
                     /locus_tag="ACHU9J_002517"
     CDS             <1..350
                     /locus_tag="ACHU9J_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1906028.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
     gene            complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHU9J_002518"
     CDS             complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHU9J_002518"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1906029.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRYDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(1733..2386)
                     /locus_tag="ACHU9J_002519"
     CDS             complement(1733..2386)
                     /locus_tag="ACHU9J_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1906030.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2386..3922)
                     /locus_tag="ACHU9J_002520"
                     /pseudo
     CDS             complement(2386..3922)
                     /locus_tag="ACHU9J_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            complement(3895..5061)
                     /locus_tag="ACHU9J_002521"
     CDS             complement(3895..5061)
                     /locus_tag="ACHU9J_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1906031.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(5071..5619)
                     /locus_tag="ACHU9J_002522"
     CDS             complement(5071..5619)
                     /locus_tag="ACHU9J_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1906032.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(5619..6326)
                     /locus_tag="ACHU9J_002523"
     CDS             complement(5619..6326)
                     /locus_tag="ACHU9J_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1906033.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHU9J_002524"
     CDS             complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHU9J_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1906034.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(7023..7733)
                     /locus_tag="ACHU9J_002525"
     CDS             complement(7023..7733)
                     /locus_tag="ACHU9J_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1906035.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHU9J_002526"
     CDS             complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHU9J_002526"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1906036.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            9152..10372
                     /gene="astC"
                     /locus_tag="ACHU9J_002527"
     CDS             9152..10372
                     /gene="astC"
                     /locus_tag="ACHU9J_002527"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1906037.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10369..11403
                     /gene="astA"
                     /locus_tag="ACHU9J_002528"
     CDS             10369..11403
                     /gene="astA"
                     /locus_tag="ACHU9J_002528"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1906038.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            11400..12878
                     /gene="astD"
                     /locus_tag="ACHU9J_002529"
                     /pseudo
     CDS             11400..12878
                     /gene="astD"
                     /locus_tag="ACHU9J_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            12875..14218
                     /gene="astB"
                     /locus_tag="ACHU9J_002530"
     CDS             12875..14218
                     /gene="astB"
                     /locus_tag="ACHU9J_002530"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1906039.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            14211..15179
                     /gene="astE"
                     /locus_tag="ACHU9J_002531"
     CDS             14211..15179
                     /gene="astE"
                     /locus_tag="ACHU9J_002531"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1906040.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         3524 a         3983 c         4194 g         3514 t
ORIGIN      
        1 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
       61 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
      121 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
      181 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
      241 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
      301 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
      361 atttgctact gtccgggccg cgttcaccgg ttgctaccgg attttttgga tcgctgctcc
      421 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
      481 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgctctggtt
      541 tgatattcca cgctttccac atagcggtaa tatcatcggc gctgcgcatg gtgccatccg
      601 ggttatgaaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
      661 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
      721 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
      781 tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
      841 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
      901 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
      961 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atagcgaatg ccgtgttttg
     1021 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     1081 cgttggtatc gatgtagtca gcaccgggaa tatggctgat aaggtaaagc ttaggagcgc
     1141 cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc tctttacctt
     1201 gttgcaggtc gtgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
     1261 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
     1321 gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagcgcc accggggcat
     1381 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcga
     1441 gccagctggc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
     1501 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgtt
     1561 gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     1621 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     1681 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
     1741 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
     1801 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ttcgcgaact tcgctgaaca
     1861 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
     1921 gtaacgcttt tggctgagcg agaagggcgc gcagcagggc cacccgcgct cgctgaccgc
     1981 ctgataatgt cgcagggtcc tggtgataag tttcagccag tcccgctcga tcaagcgcgt
     2041 ctttcacggc attgcgcctg gcagtcccct tgagcgtcga cagtagcgcc agcagtaaat
     2101 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg ccaatctgac
     2161 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
     2221 aaaactgtcc agccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
     2281 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
     2341 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggagtccttg
     2401 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
     2461 caacagttgc cataaagcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     2521 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     2581 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag
     2641 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     2701 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     2761 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatctaggt tcagccatag
     2821 cgccagcgtg tactggcccg ccaccagtgg cagatcgggc aatagaatgg gcaaccatag
     2881 ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacagga gcaaagcgat
     2941 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
     3001 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     3061 cgccagcgta gtgcctggta ataaaggtgt ggcaggcatt gcgaatgcca tctacgcggg
     3121 gaatagtgcg ccgccagctg cgccatagca gatagctcag caaaacgtag gcggcgagta
     3181 acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg tcgccctgag
     3241 ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca agaataatcg
     3301 ccacatccac gaccgaaagc gaccaggcaa caatcgccag catcgccatt gccagcgaag
     3361 gcgcgacgga gggcaacagc agccagttca ggcattgcca gcggctgtag cccagcgaat
     3421 ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccgct aagatccaca
     3481 gcagaaatgc gctttctttc actgcaaggg tgaggcccag accgatgcca aatcggtcca
     3541 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     3601 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     3661 tacgctgcca tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg
     3721 ccccgaccgc cgcgatggtt gtcgatacca gcgttgccag taatgcatgc ggtaactgtg
     3781 gatcggcaaa taacgcctgc cagtgtgtca acgacaacgc aggcgatatc agcgtgagag
     3841 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     3901 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     3961 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     4021 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     4081 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     4141 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     4201 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gcgcggggtt
     4261 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     4321 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcca
     4381 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     4441 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     4501 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     4561 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     4621 gggcgattcc gcaccttgtg tcggcactga aaaatcttcc cgcaccggca gctgtgtgtc
     4681 gacatagcgc cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt
     4741 taaggtgcgg aagttttcgc cgttcaccca gagcagatcc accgagccgc ccgttttacg
     4801 accggcagca gcttcggtct gaatgcgctt caccgcgtct gcggcatccg cgagacgaac
     4861 aatcttcagg tttatagcgt aatgggtttt catctcgccg ctaacccagt cgagatagcg
     4921 gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg gtttgccctt tggcctcatt
     4981 tttaatttcc tgccagtcac tggcatgtgt tgccaaagaa aacagcgata acaatcccag
     5041 caaccaccca caatggcgca tcttctctcc ttaagaacgt ttttccggaa tacggcacag
     5101 cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc gacaggtttt gcaggccgat
     5161 caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc gcagccagtc gggtacgcag
     5221 ggcatcatcg actgttggcg gtgtgacgct tgcggcttcg gcgtactcca gcgccagccg
     5281 ttcttcatcg ctaaagagcg ggctttgccg ccagtcagcc actgccagca gtttatcgct
     5341 gctgccggta cgctcggcga ctttcatact ggtgatatcc acacaaaact cacacaggca
     5401 catttgcgca atgcgcgcgc tgacaagcga tcgcactacc ggatcgagcg gtgagcgttt
     5461 gcgctccagc cacccaacaa acattgacac caaataaaag ataaacggga tccgccccca
     5521 ccagcgaatc gggcttagca cctcgccgta atggcgacga tgcagccacg cctgcggacg
     5581 taaaatgaaa ggaattttgc taagcggcgg taaacccatc agtcattcct cttctggcgc
     5641 tcaagccata ttttcttcgc cataaaaatc accacggtca gggcaaacag aataaacagt
     5701 ccggtgacaa accagaaagt accgcctgtt aacatgctgc ccgcccagga ataaacaata
     5761 gtcgtcggta attgaccaag cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc
     5821 agatcggcag catagctgat tggatcgaaa gggacaaaag gcaataaccg acagaccagg
     5881 atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac tgtcaagcac ggttttgccg
     5941 gttaattttt ccaccacttc gcggcccatc actctggcga taaaaaagca cagcgccgcg
     6001 ccggccatcg aactggtcca cgacagcaag ccgccccaga acgcgccaaa cagcgacgca
     6061 ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac
     6121 aagaacgaga caactgccgc cagtgcgccg taagactgaa taaagcgttc tataccctgt
     6181 tggtccacgg cggcaaacgc cgcaacgctg cgattgataa actcatggac gccgggaagc
     6241 agcgcccatg cgagcagcat agcgaacaac aggatgatga gggttatacg gtagtaccag
     6301 attttacgcg attgcatctt catcatcctt tatcctggct ttattcggtg attttaaagg
     6361 taaccgttgc cagcgtgtta tccgccggga gaactgaggc attgcctttg aatttcactt
     6421 cgccgcgttt ttccaccgca ccataagtgt atgttgcatt gctgacgatg ccgaccgggc
     6481 agctatccag acacaccagg caacccgttt tcttctcttc tgctgccgtt aaattaccgc
     6541 caaagcgcat gtccagtttc ttgccattac tgtcaacaat cacttcatcg aaggaatacg
     6601 cttttgccgc cccttgccag ttgaccgaaa tatccagttt gctgcctgtg acatgagtcg
     6661 tttctttatt atccatcgtc atgttttcgc ccggcgtacc acctgcctct ttcagggctt
     6721 cataaaatgc tttcggggtc gcataagcca taaacagcga tttatgtccg ttggagccat
     6781 ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa acgaccatta atttgcacca
     6841 acatggttac cgtatggtta ttggcatcga cgcgcagcgg ttggctgttc gaaagtccgt
     6901 cgtattccac ttttgccgcc gcattctctt tttgatcgca gccgctaagc ccggcaacag
     6961 ccagtaagca gagtgcagcc agtccttttt tccatgacac tgaataatgt tgcgacatcg
     7021 ttctatcctt cattttttgg gtgagtaagc gggctgcggc gcgaagcaga cagatccaca
     7081 tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa taaacagcgc caggcccgcc
     7141 agacagagtt gtaaaataaa gcgcagcgta atgccttcat tggcgagatc gcttgccatc
     7201 acggtataaa taacaatacc gggcagggtc gtgagtgccg aaataagggt ataaggccag
     7261 aaggcgatgg tggttaatcc gtaagcgtaa ttttgaatat tgtaaggaaa caacgggatt
     7321 aagcgggtca gaataagaaa atcaataccg ttacgcgcaa tgcctttttc aatggcctgg
     7381 aaggtatggc tatggcccac gtatttcagc agtaaatcac gtcccagcca ccgcgccagc
     7441 aggaatgagc acgaggaggc cagcgtggcg gcaattaatg agagtagtgt ccctaagagc
     7501 gggccaaaaa ctattcctcc agcgatcacc agtatgcttc ctggtaacac aaagagggtg
     7561 gcaatgatga ataacagaat atagaggcta taaccgaaca atccgctctg gcgaatgagt
     7621 gtctgtaagt ggggtaaatc ggtgagcaga tcgaataaac cgaaagcgtg gatagcgtaa
     7681 atgaccagcg caaaaataag acaggcgaaa agaaatttac gctcagcatt catcgtggag
     7741 tttcctatat caaacttgat aatagagaag tggtcagaca actaaaaacg cagtgacggt
     7801 ataacaaagg acggcaggca acaaatcatt actgacagtt tgttgatttt tctcaaggtt
     7861 aattctcctg acccagtttg agccaggaga gctgctaaat tagcggcgga aggtcgccca
     7921 gacgggggcg tgatcggacg gtttttccat gctgcgaatt tcatagtcga tgccggtttc
     7981 tacgcaacat tctgccagcg gttggctggc gagcagcagg tcgatgcgca gaccacggtt
     8041 atcgtcaaaa ccttttgagc ggtaatcaaa ccatgagaaa cgatctgctg tttgcggatt
     8101 agcatggcgg aaggtatcga ccaaccccca gctcatcagc ctgtccatcc attcgcgctc
     8161 ttccggcagg aaagagcatt taccggtacg cagccagcgc ttacggtttt cttcgccaat
     8221 gccgatatcc agatctgtag ggctgatatt catatcgccc ataatcagta ccggattatc
     8281 acgtttgagt tcggtttcca ggtagttttg cagattctga taaaactgcg cttttgccgg
     8341 gaattttatc ggatggtcgc ggctttcacc ctgcgggaag taaccgttga tcacggtgac
     8401 attacccagc agtgagggga tttccgccat aataatccgc cgctgcgcct cttcgtcgtc
     8461 accgggaaag ccgcgacgca cggcaatcgg cgtctctttg gtcagcagcg ccacgccata
     8521 atggcctttc tgcccgtgat aaaacacgtt gtagccgagc ttcgccacct cttcgagcgg
     8581 aaacatatcg tcatgaactt ttgtctcctg caggccaatc acatccggtt ggtgcttttc
     8641 gacgatggct tcaagctggt gaggtctggc gcgcaggccg ttgatattaa aagagacaaa
     8701 tttcatagtc gctgccattt attcagataa agagtgcgtg gatggtagca gaatttcgcg
     8761 ttgcttaccg cctgttgtta agtgatttga tcgatggttg ttaatgatgt caacgatggc
     8821 gcaaaaaatg cccgcttttg gtgcgcgctg cgtcagaatg gcgcagtaat ttccagtaaa
     8881 tttcgtcaat gatcacaatt ccagaattat attttacttc tgcataacat tgcgtttttt
     8941 attcttttta ttcacccaca tcgcaaacgt attcacttta tatgcacttt aaatgcatat
     9001 ggtttggtta taactccttg atttccagtt agcctccgcc gtttatgcac ttttatcact
     9061 ggctggcacg aaccctgcaa tctacattta cagcgcaaac attacttatt gttaacatat
     9121 agatagcgaa ttatttactg tagaggtcgc tatgtctcag ccaattacgc gtgaaaactt
     9181 tgatgaatgg atgatacctg tttacgctcc ggcacccttt ataccggtac gtggcgaagg
     9241 ttcgcgcttg tgggatcagc aggggaaaga gtatatcgac ttcgcgggtg gcattgcggt
     9301 gaacgcgctg ggccatgcgc atccggaact gcgtgaagcg ctgaacgaac aggcgagtaa
     9361 gttctggcat accggcaacg gttacaccaa cgagccggta ctgcgactgg cgaaaaaatt
     9421 gatcgacgcc acgtttgccg atcgcgtctt cttttgtaac tccggtgcgg aagccaacga
     9481 agcggcgcta aaactggcgc gtaaattcgc tcacgaccgc tacggcagcc ataagagcgg
     9541 tatcgtggcg tttaaaaatg cgtttcatgg tcgcacgctg tttactgtca gtgcgggtgg
     9601 gcagccagcc tattcacagg attttgcgcc actgccacca gatatccgtc atgctgcata
     9661 taacgatatt aactctgcca gcgcgctgat tgacgacgcc acctgtgcgg tgattgtcga
     9721 acccatccag ggggaaggcg gtgtggtgcc agccagcaac gcgtttttac aaggtctgcg
     9781 tgaattgtgt gaccgccaca atgcgctgtt gatttttgat gaagtacaaa ccggcgtcgg
     9841 gcgcaccggg gaactgtatg cctatatgca ctacggcgtg acacctgatc tgttaactac
     9901 cgccaaagcg ctgggcggcg gtttcccggt cggtgcgttg ttaacaaccg aagagtgcgc
     9961 cagcgtgatg accgttggca ctcatggcac cacctatggc ggcaatccgc tggcctcggc
    10021 ggtggcgggc aaagtgctgg agctcatcaa cacaccagag atgcttaatg gcgttaaaca
    10081 gcgtcacgac tggtttgttg agcgtcttaa taccattaat caccgctacg gtttgttcag
    10141 tgaagttcgc ggcttaggtt tgttgattgg ctgtgtactg aatgccgatt acgccggaca
    10201 agcgaaacag atctctcagg aggcggcgaa agcaggcgtt atggtactga ttgcgggtgg
    10261 caacgtggtg cgttttgcgc ctgcgctcaa tgtcagcgaa gaagaggtga cgaccggact
    10321 ggatcgcttt gcagctgctt gcgaacactt tgttagccga ggttcatcat gatggtcatc
    10381 cgtcccgttg agcgctcaga tgtctcggcg ctgatgcagc ttgccagcaa aacgggcggc
    10441 ggcctgacgt cgcttcccgc caatgaagcc acgctttccg cgcgtatcga aagggcaatc
    10501 aaaacctggc aaggcgaact gcccaaaagc gagcagggct atgtgttcgt gctggaagat
    10561 agcgagacag gcaccgtggc ggggatttgc gccattgagg tggcggttgg gctgaacgat
    10621 ccctggtaca actatcgggt cggcacgttg gttcacgcct caaaagagct gaatgtctat
    10681 aacgcattgc cgacgctgtt tcttagtaac gatcacaccg gcagcagcga gctgtgcacg
    10741 ctgtttctcg acccggactg gcgcaaagag ggcaacggct atttgctgtc gaaatcgcgc
    10801 tttatgttta tggcggcttt tcgcgacaag tttaatgaca aagtggttgc tgaaatgcgc
    10861 ggggtgattg acgaacacgg ctattcaccg ttctggcaaa gcctcggtaa acgcttcttt
    10921 tcgatggatt ttagccgcgt cgattttctc tgcggcaccg ggcaaaaggc atttattgca
    10981 gaactgatgc cgaaacatcc gatctatacc cactttttat cccaggaagc ccaggacgtc
    11041 atcggtcagg tacatccgca aaccgcgcct gcccgcgcgg tgctggagaa agaaggtttt
    11101 cgttaccgca actatatcga catcttcgac ggcgggccaa cgcttgagtg tgacatcgac
    11161 cgcgtgcgcg ccatccgtaa aagtcggctg gtggaagtgg cagaagggca gcctgcgcag
    11221 ggcgattttc cggcctgcct ggtcgccaat gaaaattatc accatttccg cgtggtgctg
    11281 gcgcgtaccg atccggcaac cgagcgtttg attttaaccg ccgcacaact ggatgccctc
    11341 aaatgccacg caggggatcg cgttcgtctg gtgcgcctgt gcgcagagga gaaaacagca
    11401 tgactttatg gattaacggt gactggataa cgggccaggg cgcatcgcgt gtgaagcgta
    11461 atccggtatc gggcgaggtg ttatggcaag gcaatgatgc cgatgccgct caggtcgaac
    11521 aggcttgtcg ggcagcccgt gcggcgtttc cgcgctgggc gcggctctca ttggctgaac
    11581 gtcaggtcgt tgtcgaacgc tttgccggat tgctggaaag caataaagcc gaattaaccg
    11641 cgattattgc cagagaaacg ggtaagccgc gctgggaagc ggcaaccgaa gtgacggcga
    11701 tgatcaataa aatcgcgata tcaattaagg cgtatcacgt tcgtaccggc gagcagcgta
    11761 gtgaaatgcc ggacggtgcg gcgagcctgc gacatcgccc gcacggcgtg ctggcggtgt
    11821 ttgggccgta taatttccct ggtcatttgc cgaacggaca tatcgttccg gcattgctgg
    11881 caggtaacac cattatcttt aaacccagcg aactgacacc gtggagtggc gaagcggtaa
    11941 tgcgtttatg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg cagggcgggc
    12001 gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg tttaccggta
    12061 gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag aaaattctcg
    12121 cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat atcgactcgg
    12181 ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc acctgcgccc
    12241 gccgtttatt gctcaaaagc ggagcacagg gcgatgcgtt tcttgctcgt ctggttgccg
    12301 tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt attggcgggc
    12361 tgatttctga acaggccgca cagcaggtgg ttactgccta gcagcaactg gaagcgatgg
    12421 gcggacgaac cctgcttgcg ccgcgcttat tacaatcaga gacatcgttg ctgacgccgg
    12481 ggatcattga aatgacaggc gttgctggcg taccagatga agaggtgttc ggaccgttat
    12541 tgcgcgtctg gcgttatgat tctttcgagg aagcgattct aatggcgaat aacactcgct
    12601 tcggactctc ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg
    12661 aggcgcgggt ggggattgtt aactggaaca aaccgcttac tggtgctgcc agtaccgcgc
    12721 cattcggcgg cattggtgca tccggtaacc atcgccccag cgcctggtat gccgcagatt
    12781 actgcgcatg gcctatggcg agcctggagt cggactcgtt aactttgccc gcaacgctta
    12841 accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga
    12901 cgggctggta gggctgacgc atcattacgc tggcctgtcg tttggtaatg aagcctctac
    12961 ccgtcaccgt tttcaggtgt ctaacccgcg actggcggcg aaacagggct tactgaaaat
    13021 gaaaaccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg agcgtccgtt
    13081 tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac tggaaaaagt
    13141 tgcacgccag gcaccgcact ggctttccag cgtcagctcc gcttcgccaa tgtgggtagc
    13201 caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc atctcaccgt
    13261 tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg aatcgctgtt
    13321 aaaagctatt tttaacgacg aagagaaatt tagcgtccat tcggcgttgc cgcaggtagc
    13381 gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt acggtgagcc
    13441 gggtatgcaa ctttttgtct acgggcgaga agaaggcaat gatacccggc cttcccgtta
    13501 tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc aggtgaatcc
    13561 ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagggtg tttttcataa
    13621 tgacgtgatt gccgtaagta accgccaggt gctgttttgc caccaacagg cgttcgctcg
    13681 ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg cgatagaagt
    13741 tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta acagccaact
    13801 gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc gggaacacgc
    13861 cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta gcgaactaaa
    13921 agtctttgat ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg
    13981 ggtagtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga tgaacgatac
    14041 gctgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgcc ttactgctgc
    14101 cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg tattgagcca
    14161 attactgaat ctcggttcgg tttatccgtt ccagcgcgag ggagggggca atggataatt
    14221 ttcttgctct gaccttaacg ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg
    14281 ttcgctggcg ctggctgggc gatggtgtgc tggaactgac gccattaacg ccaccgcaag
    14341 gcgcgctggt gatttctgcg gggatccacg gtaatgagac ggcacctgtg gaaatgctgg
    14401 acgcgttgct tggcgcgata tctcacggcg agatcccgtt acgttggcgg ttgctggtga
    14461 tcctcgggga tcctcctgcg ctgaagcaag ggaaacgtta ttgccatagc gatatgaacc
    14521 gaatgtttgg cggtcgttgg cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg
    14581 aactggaaca gtgcctggaa gatttttatg accagggcaa agaatctgtg cgttggcacc
    14641 ttgatctaca taccgcaatt cgtggctcct tgcatcctca gttcggtgta ttaccgcaac
    14701 gcgacattcc ctgggacgag aaatttctga cgtggctggg tgcggcgggg ctggaggcgc
    14761 tggtgttcca tcaggagcct ggtggtacgt ttacccattt cagcgccaga cattttggcg
    14821 cgctggcctg tacgctggaa cttggcaaag cgttgccctt tgggcaaaac gatcttcgcc
    14881 agtttgcagt aactgccagc gcaattgctg cgctgctatc tggtgagagt gtcggtatcg
    14941 tgagaacacc gccgctccgt tatcgggtgg tttcgcaaat tactcgccac tcgccgtcct
    15001 tcgaaatgca tatggcaagt gacacgctga attttatgcc gtttgagaaa ggaacattgc
    15061 tggcgcagga cggagaggaa cgttttaccg taacccatga tgtagagtat gtgttattcc
    15121 ctaatccgtt ggtagcgttg ggattacgcg cgggattaat gctcgaaaaa ataagctaat
    15181 acttacccgc agaaatcatt ctgcgggtat taagt
//
LOCUS       DAZPJP010000091        15176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPJP010000091 DAZPJP010000000
VERSION     DAZPJP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.091"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            4..873
                     /locus_tag="ACHU9J_002532"
     CDS             4..873
                     /locus_tag="ACHU9J_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1906041.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1076..1429)
                     /locus_tag="ACHU9J_002533"
     CDS             complement(1076..1429)
                     /locus_tag="ACHU9J_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1906042.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAVMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1567..3213)
                     /gene="groL"
                     /locus_tag="ACHU9J_002534"
     CDS             complement(1567..3213)
                     /gene="groL"
                     /locus_tag="ACHU9J_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1906043.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3257..3550)
                     /locus_tag="ACHU9J_002535"
     CDS             complement(3257..3550)
                     /locus_tag="ACHU9J_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1906044.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3826..5082
                     /gene="yjeH"
                     /locus_tag="ACHU9J_002536"
     CDS             3826..5082
                     /gene="yjeH"
                     /locus_tag="ACHU9J_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1906045.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5104..5574)
                     /locus_tag="ACHU9J_002537"
     CDS             complement(5104..5574)
                     /locus_tag="ACHU9J_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1906046.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5911..7347
                     /gene="aspA"
                     /locus_tag="ACHU9J_002538"
     CDS             5911..7347
                     /gene="aspA"
                     /locus_tag="ACHU9J_002538"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1906047.1"
                     /translation="MSNNIRIEEDLLGTKEVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7465..8766
                     /gene="dcuA"
                     /locus_tag="ACHU9J_002539"
     CDS             7465..8766
                     /gene="dcuA"
                     /locus_tag="ACHU9J_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1906048.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8882..9220
                     /gene="cutA"
                     /locus_tag="ACHU9J_002540"
     CDS             8882..9220
                     /gene="cutA"
                     /locus_tag="ACHU9J_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1906049.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9196..10893
                     /gene="dsbD"
                     /locus_tag="ACHU9J_002541"
     CDS             9196..10893
                     /gene="dsbD"
                     /locus_tag="ACHU9J_002541"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1906050.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10930..11505
                     /locus_tag="ACHU9J_002542"
     CDS             10930..11505
                     /locus_tag="ACHU9J_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1906051.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11612..11687
                     /locus_tag="ACHU9J_002543"
     tRNA            11612..11687
                     /locus_tag="ACHU9J_002543"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11645..11647,aa:Phe,seq:gaa)
     gene            11930..12044
                     /locus_tag="ACHU9J_002544"
                     /pseudo
     CDS             11930..12044
                     /locus_tag="ACHU9J_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12304..12603
                     /locus_tag="ACHU9J_002545"
     CDS             12304..12603
                     /locus_tag="ACHU9J_002545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR1906052.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12594..12719)
                     /gene="laoB"
                     /locus_tag="ACHU9J_002546"
     CDS             complement(12594..12719)
                     /gene="laoB"
                     /locus_tag="ACHU9J_002546"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR1906053.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12786..13841
                     /gene="cadC"
                     /locus_tag="ACHU9J_002547"
     CDS             12786..13841
                     /gene="cadC"
                     /locus_tag="ACHU9J_002547"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR1906054.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14206..>15176
                     /gene="cadB"
                     /locus_tag="ACHU9J_002548"
     CDS             14206..>15176
                     /gene="cadB"
                     /locus_tag="ACHU9J_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1906055.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3738 a         3780 c         3636 g         4022 t
ORIGIN      
        1 tacatggcca taagcatcaa gggtgttaat accggtgtta ttcgcaagag taataatttc
       61 attgcactgg cgctgaaaat taaagaacct cgcaataaag aatcgttgtt ctttatgtct
      121 gtcatggaac tgcgcgacct gctgattgcg ctggaaagtc gtctgcatca aaagcataaa
      181 ctgaatgctg ctgcacgcct tcagtatgag caagctcgcg ataaagtcat taagaaaatg
      241 gcagaaaaca tcccagaaat tctggttgat gagcttaaaa atgccgatat taatcgtcgg
      301 gttaacacgc tggaacttac cgataatcaa ggcgaaaatc ttaccttcgt attaacgcta
      361 catgacggta gcaaatgtga gttagtcgtc aatgagttgc agattgaaat gctggcgcga
      421 gcaatcattc atgcaattaa taatgcggaa atgcgtgaac tggcattacg tattacctca
      481 ctgttagatt tcctgccgtt atacgacgtt gactgccagg acaatggcaa tctggaatat
      541 gacacctatt cccaaccgga atggaaacat aacctgttcg accattatct ggcggtgctt
      601 taccgcttta aagatgaaag tggcaaagaa caatttagcg gcacagtggt gaaaacgcgt
      661 gaagcaacgc cgggtaaaga aatagaggcg atcactcgcc gaatgcttga tttcagccca
      721 cgactgaaaa aactcgcggg tgtgccatgt caggtctatg ttcgcaccgt agcggcgaat
      781 aacgcccaac cgctaaccca ggatcaatgc ttacgcgcgc tgcatcactt acgtgttcaa
      841 tccaccagca aaactgcacc acaggcgaaa taatctcatc tttgctaatg cgaggctgga
      901 gccatccagc ctgaaatgac tgataacgtg atcgttggtc atgccggatg cgacgtaaac
      961 gccttatccg gcctacaaat tctcaagcat tcaatcagtt gcaatgcctg ctgtaggcct
     1021 gataagcgaa gcgcatcagg caataatacg tttgtcaccc agcctctgga tcacatcagt
     1081 tcgggcactt ataaacctga ccgataatct ggctatccgt cgggacaaaa ctggacaaca
     1141 ttccctgcga cgggctgctg atgccataaa tcacgttacc gcccattaca gccgcctggt
     1201 tgcgcagatc gtttgctgcg ccgcgcatag aaccgccctc ttctccgtgt tgcccggaaa
     1261 gccagttgct ttgcttacct gtcgcagtac caatcagctg gcactctgcg cctggctgct
     1321 cgtccacaat gcgtacactc tgaccggcag cactcaattc gttgctggag ctacaacccg
     1381 ccatcagtag cgcggcaccg acaatccctg ctaagtattt tacgtgcatg ttattcccca
     1441 taatcaatga gctggacgcc actcgcgtcg tccgtgtctg aatcttatac tagaaagatg
     1501 accaaaagaa aaacccccag acatttctgc ccgggggttt gtttatttct gcgaggtgca
     1561 gggcaattac atcatgccgc ccatgccacc catgccgccc ataccgccag cagcgcctaa
     1621 gtcagctgca tcatttttcg gcaggtcggt aaccatgcac tcggtggtga tcatcaggcc
     1681 agccacagaa gccgcgtact gcagagcaga acgggttact ttggttgggt ccaggatacc
     1741 catgtcgatc atgttgccgt attcttcggt tgctgcgttg taaccgtagt tgccgtcgcc
     1801 gcctttaacg gtgttagcaa caacagacgg ttcttcgccg cagttcagga cgatctgacg
     1861 cagcggagct tccattgcac gcagtgcaac tttgataccc acgttctggt cttcgttctg
     1921 accacgcagg tcagccagtt tagacgctac gcggatcagc gcaacaccac caccagcaac
     1981 cacgccttct tctaccgcag cacgggtcgc gtgcagggcg tcttcaacgc gtgctttttt
     2041 ctctttcatt tcaacttcgg tagcagcacc tactttgata actgcaacgc cgcctgccag
     2101 tttcgctacg cgttcctgca gtttttcacg gtcgtagtca gaagttgctt cttcaatctg
     2161 ctgacggatc tgagcaacac ggccctggat tgcagcttct tcacccacgc catcgatgat
     2221 agtggtggtg tctttgttga tcacaacacg tttagcctga cccaggtctt ccagggttgc
     2281 tttttccagt tccataccga tctcttcaga gatcacggta ccgccagtca gggttgcgat
     2341 atcctgcagc atagctttac gacgatcgcc gaagcccgga gctttaactg cagcaacttt
     2401 cacgatgcca cgcatggtgt taacaaccag agttgccagc gcttcgcctt ctacatcttc
     2461 agcgatgatc agcagcggtt tgcctgcttt ggcaacggct tccagaaccg gcagcatttc
     2521 gcggatgttg gagattttct tgtcagccag caggatgaac gggctttcca gttctactgc
     2581 gccagtttcc ggcttgttga tgaagtaagg agacaggtag ccacggtcga actgcatacc
     2641 ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct tcaacggtga taacgccttc
     2701 tttaccgact ttgtccatcg cttcagcgat cagtttacct acggtttcgt cggagttagc
     2761 ggagatggta ccaacctgag caatcgcttt agagtcagag cacggtacgg acagcgcttt
     2821 cagttcttca actgcagcgg taacggcttt gtcgatacca cgtttcaggt ccatcgggtt
     2881 catgcccgca gcaacagctt tcagaccctc agtgatgata gcctgagcca gtacagttgc
     2941 agtggtggta ccgtcgcctg cagcgtcgtt cgctttagag gcaacttctt tcaccatctg
     3001 cgcacccatg ttttcgaact tgtcttccag ttcgatttca cgagcaacgg aaacaccatc
     3061 tttggtgatg gtcggtgcac cgaaagattt atccagaact acgttacggc ctttcggacc
     3121 gagggtaact ttcactgcat ctgccagtac gtttacgccg cgcagcattt tcacacgagc
     3181 gtcgttaccg aattttacgt ctttagctgc cattatcttt attccttaaa ttcgtatgtt
     3241 cagtgtcgtg cgaggattac gcttcaacaa ttgccagaat gtcgctttcg gacatgatca
     3301 acacttcttc attgtcgatc ttctcagatt tcacaccgta gccatcgttg aaaataacga
     3361 tgtcgccaac tttcacatcc agcggcttca cttcgccatt ttcaaggata cggccattgc
     3421 cgacagccag cacttcgccg cgggtggatt tagccgctgc agagccggtc agaacgatgc
     3481 cgccagcaga tttagtttca acttctttac gcttgacgat cacgcgatca tgcaatggac
     3541 gaatattcat tgataactct cctttgagaa agtccgtatc tgttatgggt gacgccggag
     3601 cttacgtggt ttcccggctg gtgaccagag aaatggggat gaggattcgc cccttcaagg
     3661 gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg attcacctca
     3721 catttcatcg caatttcttc atcgccgtca taagcgaatc tgattgtgct accatcgaaa
     3781 atctacgcag ttgcccaaaa tttgggcgca atcggatatc aacccatgag tggactcaaa
     3841 caagaactgg ggctggccca gggcattggc ctgctatcga cgtcattatt aggcaccggc
     3901 gtgtttgccg ttcctgcgtt agctgcacag gtagcaggca ataacagcct gtgggcgtgg
     3961 cccgttttga ttatcttagt gttcccgatt gcgattgtgt ttgcgattct gggtcgccac
     4021 tatcccagcg caggcggtgt cgcgcacttc gtcggtatgg cgtttggttc gcggcttgag
     4081 cgagtcaccg gctggttgtt tttatcggtc attcccgtgg gtttgcctgc cgcgctacaa
     4141 attgccgccg ggttcggcca ggcaatgttt ggctggcata gctggcaact gttgttggca
     4201 gaactcggta cgctggcgct ggtgtggtat atcggtactc gcggtgccag ttccagtgct
     4261 aatctacaaa ccgttattgc cggacttatc gtcgcgctga ttgtcgctat ctggtgggcg
     4321 ggcgatatca aacctgcgaa tatccccttc ccggcacctg gtaatatcga acttaccggg
     4381 ttatttgctg cgttatcagt gatgttctgg tgttttgtcg gtctggaagc atttgcccat
     4441 cttgcctcgg aatttaaaaa tccagagcgt gattttcctc gcgcattgat gattggtctg
     4501 ctgctggcag gattagtcta ctggggctgt acggtagtcg tcttacactt cgacgcctat
     4561 ggtgaacaaa tggccgcggc agcatcgctt ccaaaaattg tagtgcaact gttcggtgta
     4621 ggagcgttat ggattgcctg cgtaattggc tatctgacct gctttgccag tctcaacatt
     4681 tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc tgaccactac
     4741 ctggcacgcc tctcttctcg ccatatcccg aataatgccc tcaatgcggt gctcggctgc
     4801 tgcgtggtga gcactttggt gattcatgct ttagagatca atctggacgc tcttattatt
     4861 tatgccaatg gcatctttat tatgatttat ctgttatgca tgctggcagg ctgtaaatta
     4921 ttgcaaggac gttatcgact actggcggtg gttggcgggc tgttatgcgt tctgttactg
     4981 gcaatggtcg gctggaaaag tctctatgcg ctgattatgc tggcggggtt atggctgttt
     5041 ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt ttcgacatta
     5101 atcctagcga tcgtctttat gatcaaggcg gtcgcggtca tcatcctttc gctggtactc
     5161 accatcaaaa gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg gcatgcgaga
     5221 aaagcgcaaa tgtggcatca acttcactgt cagatgcttt tgcaccggcg gcaataaaag
     5281 cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac cggcaataat
     5341 cagcgaaaca cttttaatca tctccgccgc agggttttca cccgccgcca ttttttgctg
     5401 catcagcacg aaattcttaa agccctggtt acgtaccagt gacataccga taactgacgt
     5461 gaatataacc agcacgaggg tcagcaatac ccccaataca tgggcaacct gaataaagat
     5521 tgaaatctca atatagacat aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt
     5581 ctcctgtaat agcagccggt taaacccggc tacctgaatg ggttgcgaat cgcgttcagc
     5641 ttatattgtg gtcattagca aaatttcaag atgtttgcgc aactattttt ggtagtaatc
     5701 ccaaagcggt gatctatttc acaaattaat aattaagggg taaaaaccga cacttaaagt
     5761 gatccagatt acggtagaaa tcctcaagca gcatatgatc tcgggtattc ggtcgatgca
     5821 ggggataatc gtcggtcgaa aaacattcga aaccacatat attctgtgtg tttaaagcaa
     5881 atcattggca gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat cgaagaagat
     5941 ctgttgggta ccaaggaagt tccagctgat gcctactatg gtgttcacac tctgagagcg
     6001 attgaaaact tctatatcag caacaacaaa atcagtgata ttcctgaatt tgttcgcggt
     6061 atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac cattcctaaa
     6121 agtgtagcga atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg
     6181 gatcagttcc cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa catgaacacc
     6241 aacgaagtgc tggccaatat cggtctggaa ctgatgggtc accagaaagg tgaatatcag
     6301 tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc ctacccgacc
     6361 ggtttccgta tcgcagttta ctcttccctg attaagctgg tagatgcgat taaccaactg
     6421 cgtgaaggct ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc
     6481 cagctgcagg acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg
     6541 ctgaaagaag aagtgaaaaa catccaacgt accgctgaac tgctgctgga agttaacctt
     6601 ggcgcaacag caatcggtac tggtctgaac acgccgaaag agtactctcc gctggcagtg
     6661 aaaaaactgg ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg
     6721 acctctgact gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag
     6781 atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac
     6841 gagatcaacc tgccggaact gcaggcgggc tcttccatca tgccagctaa agtaaacccg
     6901 gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga caccactgtt
     6961 accatggcag cagaagcagg tcagctgcag ttgaacgtta tggagccggt cattggccag
     7021 gctatgttcg aatccgttca cattctgacc aacgcttgct acaacctgct ggaaaaatgc
     7081 attaacggca tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt
     7141 atcgttactt acctgaaccc gttcatcggt caccacaacg gtgacatcgt gggtaaaatc
     7201 tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa
     7261 gcggaacttg acgatatttt ctccgtacag aatctgatgc acccggctta caaagcaaaa
     7321 cgctatactg atgaaagcga acagtaatcg tacagggtag tacaaataaa gaaggcacgt
     7381 cagatgacgt gccttttttc ttgtgagcag taacttaaaa ataacaatct aatatcaact
     7441 tgttaaaaaa caaggaaggc taatatgcta gttgtagaac tcatcatagt tttgctggcg
     7501 atcttcttgg gcgccagatt ggggggaata ggtattggtt ttgcaggcgg attgggggtg
     7561 ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt catttccatt
     7621 atcatggcgg ttatcgccgc tatttctgcc atgcaggttg ctggcggtct ggactatctg
     7681 gttcatcaga cagaaaagct gctgcgccgt aacccgaaat acatcacaat cctcgcaccg
     7741 atcgtgacct atttcctgac aatctttgct ggtactggca acatctctct ggcgacactg
     7801 ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac cttgccgtcc gctgtctact
     7861 gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc agtggtttac
     7921 atgtcttccg tgatggaagg tcatggcatc agctacctcc atctgctctc cgtggtcatc
     7981 ccgtccaccc tgctggcggt tctggtgatg tccttcctgg tcactatgct gttcaactcc
     8041 aaactctctg acgatccgat ttatcgcaag cgtctggaag agggcctggt tgaactgcgc
     8101 ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt ccgtctggct gttcctgctg
     8161 ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc caagcatggg tctggttgaa
     8221 aaaccgctga tgaacaccac caacgcaatc ctgatcatca tgctcagcgt tgcaactctg
     8281 accaccgtta tctgtaaagt cgataccgac aacatcctca actccagcac cttcaaagca
     8341 ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc
     8401 aacaacatcg actggatcaa agataccgct ggtgaagtga ttcagggtca tccgtggctg
     8461 ctggccgtca tcttcttctt tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa
     8521 gcactgatgc cgatggctct ggcactgaac gtttctccgc tgaccgctgt tgcttctttc
     8581 gctgcggtgt ctggtctgtt cattctgccg acctatccga cgctggttgc tgcggtacag
     8641 atggatgaca cgggtacaac ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc
     8701 ccgggtactc tgggtgttgc cctggccgtt tgcttcggct tcgtgctggg tagcttcatg
     8761 ctgtaatgac caatcgcggg gcgttcacgc cccgctttct ttcccgccga ctaacatcct
     8821 tcccccgtcc gttgtatagt gacctctctc ttgcggttcc atctgttctt gcgaggtgtt
     8881 tatgcttgat gaaaaaagtt cgaataccgc gtctgtcgtg gtgctatgta ccgcaccgga
     8941 tgaagcgaca gcccaggatt tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc
     9001 gaccttgatc cccggcgcta cctctctcta ttactgggaa ggtaagctgg agcaagaata
     9061 cgaagtgcag atgattttaa aaactaccgt atctcaccag caggcactgc tggaatgcct
     9121 gaagtctcat catccatatc aaaccccgga acttctggtt ttacctgtta cacacggaga
     9181 cacagattac ctctcatggc tcaacgcatc tttacgctga tcctgctact ttgcagcact
     9241 tccgtttttg ccggattatt cgacgcgccg ggacgttcac attttgtccc cgcggatcaa
     9301 gcctttgctt ttgattttca gcaaaaccaa catgacctga atctgacctg gcagatcaaa
     9361 gacggttact acctctaccg taaacagatc cgcattacgc cggaacacgc gaaaattgcc
     9421 gacgtgcagc tgccgcaagg cgtctggcat gaagatgagt tttacggcaa aagcgagatt
     9481 taccgcgatc ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta
     9541 actgtcacct accagggctg tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc
     9601 gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac agcctgtgtc tgttccgcag
     9661 caagagcagc acaccgcgca attgcccttt tccgcgctct gggcgttgtt gatcggtatt
     9721 ggtatcgcct ttacgccatg cgtgctgcca atgtacccac tgatttctgg catcgtgctg
     9781 ggtggtaaac agcggctctc cactgccaga gcattgttgc tgacctttat ttatgtgcag
     9841 gggatggcac tgacttacac ggcgctgggt ctggtagttg ccgccgcagg gttacagttc
     9901 caggcggcgc tacagcaccc atacgtgctc attggcctcg ccatcgtctt taccttgctg
     9961 gcgatgtcaa tgtttggctt gtttactcta caactcccct cttcgctgca aacacatctc
    10021 acattgatga gcaatcgcca acagggcggc tcacctggcg gtgtgtttgt tatgggggcg
    10081 attgccggac tgatctgttc accatgcacc accgcaccgc ttagcgcgat tctgctgtat
    10141 atcgcccaaa gcgggaacat gtggctgggc ggcggcatgc tttatctcta tgcgttgggc
    10201 atgggcctgc cgctgatgct aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc
    10261 ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact gccgatcttc
    10321 ctgctggagc gagtgattgg tgatatatgg ggattacgct tgtggtcggc gctgggtgtc
    10381 gcattctttg gctgggcctt tatcaccagc ctacaggcca aacgcggctg gatgcgcgtg
    10441 gtgcaaataa tcctgctggc agcggcattg gttagcgtgc gcccacttca ggattgggca
    10501 tttggtgcga cgcataccgc gcaaactcag acgcatctca actttacaca aatcaaaacg
    10561 gtagatgagt taaatcaggc gctcgttgaa gccaaaggca aaccggtgat gttagatctt
    10621 tatgccgact ggtgcgtcgc ctgtaaaaag tttgagaaat acaccttcag cgacccgcag
    10681 gtgcaaaaag cgttagcaga cacggtctta cttcaggcca acgtcactgc taacgacgca
    10741 caagatgtgg cgctgttaaa gcatcttaat gtccttggac taccgacaat tctctttttt
    10801 gacggacaag gccaggagca tccacaagca cgcgtcacgg gctttatgga tgctgaaacc
    10861 ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg aaagacggag
    10921 gaaaatagcg tgcaacgtga agatgtactg ggagaagccc tgaaattatt agaattacaa
    10981 gggattgcca acaccacgct ggagatggtt gctgaacgtg tggattatcc actggacgag
    11041 ctacgccgct tctggccaga caaagaggca atcctctacg atgcgctgcg ttatcttagc
    11101 caacagatag atgtctggcg tcgtcagctg atgcttgacg aaacgcaaac cgccgaacaa
    11161 aagctgctgg cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc
    11221 tgtctattta tcgctgcctg tacgttttat cccgaccctg gccaccctat tcatcaactg
    11281 gccgatcagc aaaaaagcgc ggcctacgat ttcacccacg aactgttaac cacgctggaa
    11341 gttgacgatc cggcgatggt agcaaagcag atggaactgg tgctggaagg ctgtttaagc
    11401 cgaatgctgg tgaatcgtag ccaggcggat gtcgacaccg cacatcggct ggcggaagat
    11461 atcctgcgct tcgcccgctg ccgccagggt ggtgcactga cctgacagaa acacagaaaa
    11521 gaagcgattt gccgcaatct taagcagttg aatcgctttt actgaaatta ggttgacgag
    11581 atgtgcagat tacggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg
    11641 gattgaaaat ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatataaac
    11701 ggacctccac ggaggtccgt ttttcgtttc aggacgccac gatttaagcg ttctgcctcc
    11761 aaatcaattc taccgaactc aaccagattc tccccacatc accagcattt tgcgggtata
    11821 tcccaattcg ggaaaatttg ttcctgagcg atagcactga ctgacgtgaa atgtcgtgcg
    11881 gccccgtgat gttgttgaac gtcaaatgac gtcatcagga gcgtaacgca tccataaagc
    11941 acaacaccgg tctgaacggc gactgatgag attttctgaa tgagaacaaa gagaaatgta
    12001 tcagtccgct tgctcatgca aagactaaca atccattaaa atagtaagcg ctccggacaa
    12061 ttttccatgg attattttct acacattttt ctttggcaaa gatgatgaat tttgatggta
    12121 aggaatatta cttctggttc tcagtaaaat cctttcgtaa tactatgtaa tcaagaagtt
    12181 tatggctggt aaaaataacg tcttgcattc accaataaca tgtaaataaa cccatctatt
    12241 gatggtaaaa ataggtcatg gcaattatca ttgcaccatt cccttttcga atgagtttct
    12301 attatgcaac aacctgtagt tcgcgttggc gaatggcttg ttactccgtc cataaaccaa
    12361 attagccgca atgggcgtca acttaccctt gagccgagat taattgatct tctggttttc
    12421 tttgctcaac acagtggcga agtacttagc agggatgaac ttatcgataa tgtctggaag
    12481 agaagtattg tcaccaatca cgttgtgacg cagagtatct cagaactacg taagtcatta
    12541 aaagataatg atgaagatag tcctgtctat atcgctactg taccaaagcg cggctataaa
    12601 tgaatggtgc cggttatctg gtacagcgaa gaagagggag aggaaataat gctatcttcg
    12661 cctcccccta taccagaggc ggttcctgac acaaattctc cctcccacag tcttaccatt
    12721 caaaacaccg caacgccacc tgaacaatcc ccagttaaaa gcaaacgatt cactaccttt
    12781 tgggtatggt ttttttcctg ttgtcgttag gtatctgtgt agcactggta gcgttttcaa
    12841 ctcttgatac acgtcttcct atgagcaaat cgcgtatttt gctcaatcca cgcgatattg
    12901 acattaatat ggtaaataaa agttgtaaca gctggagttc cccgtatcag ctctcttacg
    12961 cgataggcgt gggtgatttg gtggcgacat cacttaacac cttctccacc tttatggtgc
    13021 atgacaaaat caactacaac attgatgaac cgagcagttc cggtaaaaca ttatctattg
    13081 cctttgttaa tcagcgccaa taccgtgctc aacaatgctt tatgtcggta aaattagtag
    13141 acaatgcaga tggttcaacc atgctggata aacgttatgt catcactaac ggtaatcagc
    13201 tggcgattca aaatgattta ctggagagtt tatcaaaagc gttaaaccaa ccgtggccac
    13261 aacgaatgca ggagacgctc cagcaaattt tgcctcatcg tggtgcgtta ttaactaatt
    13321 tttatcaggc tcatgattat ttactgcatg gcgatgacaa atcattgaat cgtgccagtg
    13381 aattattagg tgagattgtt caatcatccc cagaatttac ctacgcgaga gcagaaaaag
    13441 cattagttga tatcgtgcgc cattctcaac accctttaga tgaaaaacaa ttagcagcac
    13501 tgaacacaga aatagacaac attgttacac tgccggaatt gaacaacctg tccattatat
    13561 atcaaataaa agcggtcagt gctctggtaa aaggtaaaat agatgagtct taccaggcga
    13621 taaatactgg cattgatctt gaaatgtcct ggctaaatta tgtgttgctt ggcaaggttt
    13681 atgaaatgaa ggggatgaac cgggaagcag ctgatgcata tctcactgcc tttaatttac
    13741 gcccaggggc aaacaccctt tactggattg aaaatggtat tttccagact tctgttcctt
    13801 atgttgtacc ttatctcgac aaatttctcg cttcagaata agtaactccg ggttgattta
    13861 tgctctggaa tatttgttgt tgagtttttg tatgttccca ttggtataat atgttgcggc
    13921 aatttatttg ccgcataatt tttattacat aaatttaacc agagaatgtc atgcaatcca
    13981 ttgtaaacat tgaatgttta tcttttcatg atatcaactt gcgatcctga tgtgttaata
    14041 aaaaacctca agttctcact tacagaaact tttgtgttat ttcacctaat ctttaggatt
    14101 aatccttttt tcgtgagtaa tcttatcgcc agtttggtct ggtcaggaaa tagttataca
    14161 tcatgacccg gactccaaat tcaaaaatga aattaggaga agagcatgag ttctgccaag
    14221 aagatcgggc tatttgcctg taccggtgtt gttgccggta atatgatggg gagcggtatt
    14281 gcattattac ctgcgaacct agcaagtatc ggtggtattg ctatctgggg ttggattatc
    14341 tctattattg gtgcaatgtc gctggcgtat gtatatgccc gactggcaac aaaaaacccg
    14401 caacaaggtg gcccaattgc ttatgccgga gaaatttctc ctgcatttgg ttttcagaca
    14461 ggtgttcttt attaccatgc taactggatt ggtaacctag cgattggtat taccgctgta
    14521 tcttatcttt ccaccttctt cccagtatta aatgatcctg ttccggcggg tatcgcctgt
    14581 attgctatcg tctgggtatt tacctttgta aatatgctcg gcggtacctg ggtaagccgt
    14641 ttaaccacta ttggtctggt gctggttctt attcctgtgg tgatgactgc tattgttggc
    14701 tggcattggt ttgatgcggc aacttatgca gctaactgga atactgcgga taccactgat
    14761 ggtcatgcga tcattaaaag tattctgctc tacctgtggg ccttcgtggg tgttgagtcc
    14821 gcagcagtaa gtactggtat ggttaaaaac ccgaaacgta ccgttccgct ggcaaccatg
    14881 ctgggtactg gtttagcagg tattgtttac atcgctgcga ctcaggtgct ttccggtatg
    14941 tatccgtctt ctgtaatggc ggcttccggt gctccgtttg caatcagtgc ttcaactatc
    15001 ctcggtaact gggctgcgcc gctggtttct gcattcaccg cctttgcgtg cctgacttct
    15061 ctgggctcct ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa cgacggtaac
    15121 ttcccgaaag tttatggtga agtcgacagc aacggtattc cgaaaaaagg tctgct
//
LOCUS       DAZPJP010000092        15170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPJP010000092 DAZPJP010000000
VERSION     DAZPJP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.092"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHU9J_002549"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHU9J_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1906056.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHU9J_002550"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHU9J_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1906057.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHU9J_002551"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHU9J_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1906058.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHU9J_002552"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHU9J_002552"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1906059.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHU9J_002553"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHU9J_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1906060.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHU9J_002554"
     CDS             complement(8214..9107)
                     /locus_tag="ACHU9J_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1906061.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHU9J_002555"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHU9J_002555"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1906062.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHU9J_002556"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHU9J_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1906063.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHU9J_002557"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHU9J_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1906064.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHU9J_002558"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHU9J_002558"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1906065.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHU9J_002559"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHU9J_002559"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1906066.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15170)
                     /locus_tag="ACHU9J_002560"
     CDS             complement(14275..>15170)
                     /locus_tag="ACHU9J_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1906067.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGRAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3558 a         4284 c         3741 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggccc gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
//
LOCUS       DAZPJP010000093        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPJP010000093 DAZPJP010000000
VERSION     DAZPJP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.093"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHU9J_002561"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHU9J_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1906068.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHU9J_002562"
     CDS             complement(1556..1879)
                     /locus_tag="ACHU9J_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1906069.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHU9J_002563"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHU9J_002563"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1906070.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHU9J_002564"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHU9J_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1906071.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHU9J_002565"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHU9J_002565"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1906072.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHU9J_002566"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHU9J_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1906073.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHU9J_002567"
     CDS             7479..8516
                     /locus_tag="ACHU9J_002567"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1906074.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHU9J_002568"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHU9J_002568"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1906075.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHU9J_002569"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHU9J_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1906076.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHU9J_002570"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHU9J_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1906077.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHU9J_002571"
     CDS             10687..11568
                     /locus_tag="ACHU9J_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1906078.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHU9J_002572"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHU9J_002572"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1906079.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHU9J_002573"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHU9J_002573"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1906080.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHU9J_002574"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHU9J_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR1906081.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPJP010000094        14619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPJP010000094 DAZPJP010000000
VERSION     DAZPJP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.094"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            71..1606
                     /gene="lsrA"
                     /locus_tag="ACHU9J_002575"
                     /pseudo
     CDS             71..1606
                     /gene="lsrA"
                     /locus_tag="ACHU9J_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1600..1776
                     /locus_tag="ACHU9J_002576"
                     /pseudo
     CDS             1600..1776
                     /locus_tag="ACHU9J_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1784..1873)
                     /locus_tag="ACHU9J_002577"
                     /pseudo
     CDS             complement(1784..1873)
                     /locus_tag="ACHU9J_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHU9J_002578"
     CDS             complement(2080..3528)
                     /gene="uxaB"
                     /locus_tag="ACHU9J_002578"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1906082.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3755..4705)
                     /locus_tag="ACHU9J_002579"
     CDS             complement(3755..4705)
                     /locus_tag="ACHU9J_002579"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1906083.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4844..5203)
                     /locus_tag="ACHU9J_002580"
     CDS             complement(4844..5203)
                     /locus_tag="ACHU9J_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1906084.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHU9J_002581"
     CDS             complement(5203..6129)
                     /gene="glsB"
                     /locus_tag="ACHU9J_002581"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1906085.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHU9J_002582"
     CDS             complement(6193..7581)
                     /gene="sad"
                     /locus_tag="ACHU9J_002582"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR1906086.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7682..8563
                     /locus_tag="ACHU9J_002583"
     CDS             7682..8563
                     /locus_tag="ACHU9J_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1906087.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8640..9755
                     /gene="yneK"
                     /locus_tag="ACHU9J_002584"
     CDS             8640..9755
                     /gene="yneK"
                     /locus_tag="ACHU9J_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1906088.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9905..11095
                     /gene="ydeA"
                     /locus_tag="ACHU9J_002585"
     CDS             9905..11095
                     /gene="ydeA"
                     /locus_tag="ACHU9J_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1906089.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(11120..11785)
                     /gene="marC"
                     /locus_tag="ACHU9J_002586"
     CDS             complement(11120..11785)
                     /gene="marC"
                     /locus_tag="ACHU9J_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1906090.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11997..12431
                     /gene="marR"
                     /locus_tag="ACHU9J_002587"
     CDS             11997..12431
                     /gene="marR"
                     /locus_tag="ACHU9J_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1906091.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12451..12834
                     /gene="marA"
                     /locus_tag="ACHU9J_002588"
     CDS             12451..12834
                     /gene="marA"
                     /locus_tag="ACHU9J_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1906092.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12866..13084
                     /gene="marB"
                     /locus_tag="ACHU9J_002589"
     CDS             12866..13084
                     /gene="marB"
                     /locus_tag="ACHU9J_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1906093.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(13141..14580)
                     /locus_tag="ACHU9J_002590"
     CDS             complement(13141..14580)
                     /locus_tag="ACHU9J_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1906094.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3785 a         3745 c         3392 g         3697 t
ORIGIN      
        1 aggttatgaa caaattaaaa gcagaaatac atttgttcaa aactcacctg caaaactgaa
       61 cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg cccgctcggt
      121 ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt tgcatcaagg
      181 ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa tgaagattat
      241 tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca actacgtcag
      301 attaacgcca gttcatgctc atcagctggg tatttatctc gttccccagg aaccgctgct
      361 tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa aacagctctc
      421 catgcagaaa atgaagaact tgctggcggc gctgggctgc tagtttgatc tgcatagtct
      481 ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg gcctgatgcg
      541 cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg cggaaaccga
      601 acgcttgttt cgtcgcttgc aagagctgct tgctactggc gtgggtattg tttttatctc
      661 gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc gcgacggaac
      721 catcgcctta agcggcaaaa ccagcgaact gtctaccgat gacattattc aggccatcac
      781 gccagtggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg agttacctgg
      841 caaccgccca caacatgccg ccggaacgtc ggtgctgaca ctagaaaatc tgaccggcga
      901 aggtttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggtc tggccgggct
      961 ggtgggggcc ggacgcacag aactggccga gacgctctat ggtctgcgta ctttgcgtgg
     1021 cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag aacgtttact
     1081 gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc tcgatgcttc
     1141 gctggcctgg aacgtctgcg cccttactca taaccttcgt ggattctggg cgaaaaccgc
     1201 gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat ttaaccaacc
     1261 ggaacaagct gcacggacat tatccggtgg caaccagcaa aaaatcctga ttgccaaatg
     1321 cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg tggatgtctc
     1381 ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcacagaatg tggcagtgct
     1441 gcttatctcc tctgatctgg aagagatcga actgatggca gatcgtgtgt atgtgatgca
     1501 tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg agactattat
     1561 gcgcgttgcc ttcggcgata gtcagcgtca ggaggcgtca tgctgaagtt tattcagaac
     1621 aaccgtgaaa tcacggcact gctggcggtg gtgctgctgt ttgtattacc cggttttctc
     1681 gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc gcaaatcctg
     1741 atcctgctgg caatgggcgc gacgctggta atgcttatgc gcaaagtagt aagcgcgatg
     1801 atatcttgga taatactgag ccgtgccaga caaaaatacg gcgcagccag tgttgttgtg
     1861 gacgaacaat catggttatc ttcaggcgtg aaaaagtcgc cctattaaac gtgattacga
     1921 tcacattctc aagacgctac ttacaaatta cctacaagct tacaacatta acaggcgctg
     1981 catgtggcat ctgttaatga taaaggcaca taggatgttg taactaatat agtgaaataa
     2041 gaaacccggt caaaaccggg ttcagaagta acggtgttat tagcacaacg gacgtacagc
     2101 ttcgcgcatc cctttttcaa gaatcgcatc caggtcatta gcaacctgct cgaccagacc
     2161 aggcacttgc gtcaggtcct gctcccagtg gtctttttct gccaacacaa tcgctaccag
     2221 ttcctgggta ccgataacat ggtcacgatg ctggctccag agttgctgat aacgttccag
     2281 ccagtgtgca tcgtcctgta ccggatatgt ttctccgtta cgctcaccac gatagaacgc
     2341 aatcaatgcc gctaatgcga aagtaaggcg cgccggaagt gtgccgtttg ccttctgccc
     2401 tgccagcagc tgcggcagga tgcgggtgcg gaacttggtc ataccgttga gcgcgataga
     2461 cagcagctga tgcttatacg ggttacggaa acgcccggtg actgcactgg cgaaagattc
     2521 cagttcatca cgaggcaaat ccagtaccgg aataatttct tcgtagatgg ctttttcaac
     2581 gaatgcgcaa atttcagcat cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa
     2641 cgccaccggc accagcgcgg tgtgcgcacc gttgaggatt gccactttgc gttctttata
     2701 cggtttaata tcgtcaacaa tcagcacgtt gagcggatat ttgtccaggc gcagctcagt
     2761 cgctaaggat ttcggcccct gaatcacaaa caggtaaaag tgttcagcgg tgtcgaggaa
     2821 accatcgtga taacccagtt cttcttccag tttagccact tcatcgcgcg gataaccggt
     2881 aacgatacgg tccaccagcg tagaacagaa gctgttagcc tgatccagcc attgaataaa
     2941 tgcttctggc agtgcccact cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc
     3001 attgtagtcg atcaactcac acgggatgat gatccaacct ttatccagcg caccgttgaa
     3061 atggctaaag cgttcgaaca gcagacgggt cagttttgcc ggatagctta tcgctggcgc
     3121 gtcatcgaat ttatcgcccg catggtagct gatccccgct tcggtggtgt tagagaaaac
     3181 aaagcgcatt tccgggttgt gcgccagttt caggaattca tcgtattcac tgtagacgct
     3241 gatttcacga ttaaccgagc gaatcagacg cgcgtcgctg accgcttccc ccttctcatt
     3301 caggccacgg ataatggtgg tatacagacc atcctgcgtg ctcagtgacg gcgggaatga
     3361 agtttcaatc ggacgaacaa cgaccacgcc agaattcaga tcggtgtgct cattcaggag
     3421 atcgatttgc cagtcaacaa aggcgcgcag gaagttacct tcaccaaact gaatgatacg
     3481 ttctggatac tgtgcaccgg gaaaatcgcg acgatttagt gttttcacaa tgggttccct
     3541 tctgattagt catacaacct gtttgaattg gtacgacagg ttagcaaact ttaatacgcc
     3601 gaacccctgt tttgatcaac tcctgatgat taatgagcag ttttatgaga aaagtgtggc
     3661 gcggatcatg gtttaatcga ggaaatacgc cttttcctgg atcataaagt ggtagaacac
     3721 attgcattca aatcgcgcgg aatgaataaa gatgtcagac aacttcctca ccgtaacgca
     3781 tagtgctggt acggttgcgc ccatctttct tcgagcgata cagacacgta tcggcttcga
     3841 ccatcaattt attaaaatca tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc
     3901 caatacttac cgtgagataa agcgtttttt gttgccaggt gaatggttgc agttcaacgc
     3961 ctttacggat tttttccgcc attagcagac catcgacagg attcaccgac ggcactgcaa
     4021 cagcaaattc ttcgccgccc atgcgcgcca ccagcccctt atcaccgaca atcttctgaa
     4081 tatgccgggc aaacacgctt aacactttat cgccacattc atgtccatag ttatcgttaa
     4141 tgcttttgaa atagtcgata tcaagcagca tgaccgtcag aggcacgatc aacacgcccg
     4201 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
     4261 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
     4321 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
     4381 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
     4441 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
     4501 aatgcagtcg attaagccag acatagcggg caaacccccc cgccattacg ccattcagcg
     4561 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
     4621 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
     4681 aagttgaaat ggggtgtaca tgcattaatt atctgtccat acaattgcgg gtggcaatta
     4741 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
     4801 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
     4861 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
     4921 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
     4981 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
     5041 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
     5101 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
     5161 ttgaacggga taaacgcgcg aagagtggat cgagagactg cattaataaa ccgaacgccc
     5221 taactgtttc gtcaattgtt caaggacggc gatacccgca agcgagttac ctgcatcatc
     5281 cagttccgga ctccagactg cgatggccat ttcatgcgga acaatcgcca caataccgcc
     5341 accaacgcca gatttcgccg gtagtcccac ccgccaggca aactcccccg cgttctggta
     5401 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg
     5461 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gagccagctc
     5521 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc
     5581 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
     5641 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
     5701 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
     5761 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgtt ccatttccag
     5821 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
     5881 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
     5941 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
     6001 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
     6061 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
     6121 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
     6181 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacaga attcgtgtaa
     6241 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
     6301 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
     6361 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
     6421 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacggggcc
     6481 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
     6541 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
     6601 cagggttttc tctacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
     6661 gagagtgttc tcttcgtcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
     6721 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
     6781 tacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
     6841 ggcatcgtta agcacaataa acggatccga accgcccagt tccagtacgc attttttcag
     6901 tgccgctcca gcctgtgcgc caatagccgc tccagcacga acacttccgg tcaccgtgac
     6961 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     7021 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
     7081 tcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     7141 agcgccacgc atcacctgcc ataacggaaa attccacggc ataatcgcca gaatcgtccc
     7201 caacggtcga tactcaataa ccgcctgctg attttccacc agtgtaggtt ccgccttcag
     7261 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
     7321 gcgcgcctgg ttgattggtt tgcccatttc gcgggtgatc atttgcgcca tttcttcgct
     7381 acgggcgcgc agagccttac cgatatcacg cattttttca gcacgataat ctatatttgt
     7441 ctcgcgccag tcgcgaaagc ctgctgccgc cagctcaagt gcgttttcga tatcgttagc
     7501 gccagcccac ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
     7561 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
     7621 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
     7681 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
     7741 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
     7801 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
     7861 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
     7921 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
     7981 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
     8041 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
     8101 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
     8161 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
     8221 taacggcagt aacatttatg ccttccgcac caattgttcg tatcgtcgcc acttcgagag
     8281 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
     8341 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
     8401 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
     8461 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
     8521 actactggat gtgtctgact cggcaaaaca gagttaccaa tgaactattt tgatagttct
     8581 tgcggctaat atgctctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
     8641 tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt cccgttcgta
     8701 atattgcacc acaggtaaaa gaagttttaa aggactttat tgatgcgcta agtacaataa
     8761 tatgtgatga agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta
     8821 ataaccttga taacttatca tatattcaga ggacttcttt ccggggtaac gacacgctat
     8881 acaatgaaaa ggttcagttt aaactcactt atcccgtcaa gaatggaaga cacaaagaaa
     8941 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
     9001 atggagaaat taatattttt tgcgctccca acccaaagcc tgtcactatg gggcgcgtat
     9061 ttcagaccgg cgtcgagcgt gtgctctttc tttttctgaa tgattttatt gaacaatttc
     9121 caatgttcaa ccctggcgtt ccgatcaaaa tagcgcatac accacatatt gaacccctgc
     9181 ctccggatca ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
     9241 ttatctctcg cggtaatttt gtcatactcc cccgattatg gaataactct gaggttcaca
     9301 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acggacagcg
     9361 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
     9421 tacccgaagt ctgcatctgc tttttattcc ttttagcaga acaagaatct cctcattttc
     9481 aggacttgtt tttatttttc gccaatatgt tattgcacta tcaccaattt atgaatccca
     9541 atgaaagtga tttgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
     9601 agcatatggc gcgcaggacc ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta
     9661 ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt cagaccacct atacccgcaa
     9721 cctccaaaac gccagacctc cctgaacgtc attaaaccgt gatgttaccg actctctgac
     9781 gcggaacaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa atacattgta
     9841 ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca cccgataatc
     9901 tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc
     9961 agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc tctctgacat
    10021 tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatc atgttgacca tttacgcatg
    10081 ggtagtagcg ctaatgtcat tgccttttat gttaatgacc agtcaggttg aacggcgcaa
    10141 attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt ttttatcgtg
    10201 gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg cgattttctg
    10261 gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcggg cacaggcctt
    10321 gagtttaatt gccaccggta cagcactggc gatggtctta ggtttacctc tcgggcgcat
    10381 tgtggggcag tatttcggtt ggcgaatgac cttcttcgcg attggtattg gggcgcttat
    10441 cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt ccggttcatt
    10501 gaaaagcctc ccgctattgt tccgccgccc ggcattgatg agcatttatt tgttaactgt
    10561 ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg tgcaaaacat
    10621 tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg gtgcgggcat
    10681 tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt tggtgagtac
    10741 ggcgattgcg ctgttgctgg tgtgtctggc actgctgcta cctgcggcga acagtgaaat
    10801 acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg ggcttggtat
    10861 gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg cgctattctc
    10921 cggcatattt aatattggaa tcggggcggg tgcgttggta ggtaatcagg tgagtttgca
    10981 ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcttttgccg cgttaatatg
    11041 gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagacgc aatagttgaa
    11101 aggcccattc gggccttttt taatggtacg ttttaatgat ttccaggatg ccgttaataa
    11161 taaactgcac acccatacat accagcagga atcccatcag acgggagatc gcttcaatgc
    11221 cacccttgcc caccagccgc ataattgcgc cggagctgcg taggcttccc cacaaaataa
    11281 ccgccaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca gcgaaggttg
    11341 aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc ggaccagcgg
    11401 tacttggcat tgctagcggc acaaaggcaa tattggcact gggttcatct tccagctctt
    11461 ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa
    11521 aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc gaaatgccaa
    11581 atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca aatacgtaca
    11641 ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct gccaggccaa
    11701 gaaatagcgc gacagttgtt aatgggttag ctaacggcag caacaccacc agccccaggc
    11761 caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg ggttatcagc
    11821 gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct tgcatcgcat
    11881 tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt catttgactt
    11941 atacttgcct gggcaatatt atcctctgca actaattact tgccagggca actaatgtga
    12001 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
    12061 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
    12121 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
    12181 aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg gtctgtaaag
    12241 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
    12301 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
    12361 aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg cttaagaaag
    12421 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
    12481 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
    12541 gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc
    12601 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
    12661 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
    12721 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
    12781 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaatag ctagttgata
    12841 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    12901 tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    12961 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    13021 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    13081 gtagttattg tcaggctctg atcacagagc ctgacatctc cgtaatatct tcctcaagtc
    13141 tcaaatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg acgctttacg
    13201 tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca ttccgtagcg
    13261 ctttttcatt tcccctgttc cggcagaaac caggtcaatg cagccccacg gggtgtagcc
    13321 aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat gggaagcaaa
    13381 gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt caaccgcacc
    13441 aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc agttgagtga
    13501 ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga tataagggtt
    13561 gctgaccaaa tctcgtggtt caacataatc cagttgcggg ttatcgggag aaaattgtgt
    13621 cgtaaaagac atgtagtagc taaaaccgat aaagtcgaca catccactgg caagaatcgc
    13681 gttatcttct ggtgtgatat cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg
    13741 ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat gcatcgcttt
    13801 cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatggggc acatggcgat
    13861 catacagcca atgttaaatt caggattgat cgattttcca gtctgtaccg ccagggcact
    13921 ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat tgatatcttc
    13981 ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac
    14041 ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa
    14101 gaccgttgat gcgaagcgga taaaaaagtc gatcagctta cggtttcgcc agccaccata
    14161 ttttgtcacc agatgataag gcatctcaaa atgcgaaagc gtcaccacag gttccattcc
    14221 ttgcttcagg cattcatcga acagatcatc ataaaattgt aaaccctctt cattcggctc
    14281 ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa
    14341 tcccatctcg gcaaataact gaatatctgt tttatagcga tgataaaaat caattgcttc
    14401 atgattggga taattaagcc cgtcgataac gccttctgtc acttcacgcg gcaccccgtg
    14461 agcgccagca gtcatcacat cagcgatact gatgcctttt cctccttcat tccagccacc
    14521 ttccaactga tgcgcggcta ccgcgccacc ccataaaaaa ccttttttaa atcctgacat
    14581 acacaactcc ttaactaaaa tagattacca ccagacttc
//
LOCUS       DAZPJP010000095        14436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPJP010000095 DAZPJP010000000
VERSION     DAZPJP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.095"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHU9J_002591"
     CDS             <1..161
                     /locus_tag="ACHU9J_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1906095.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHU9J_002592"
     CDS             complement(199..1260)
                     /locus_tag="ACHU9J_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1906096.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHU9J_002593"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHU9J_002593"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1906097.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHU9J_002594"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHU9J_002594"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1906098.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHU9J_002595"
     CDS             complement(3454..4155)
                     /locus_tag="ACHU9J_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1906099.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHU9J_002596"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHU9J_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1906100.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHU9J_002597"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHU9J_002597"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1906101.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHU9J_002598"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHU9J_002598"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1906102.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHU9J_002599"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHU9J_002599"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1906103.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8402..10468
                     /gene="ppk1"
                     /locus_tag="ACHU9J_002600"
     CDS             8402..10468
                     /gene="ppk1"
                     /locus_tag="ACHU9J_002600"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1906104.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10473..12014
                     /gene="ppx"
                     /locus_tag="ACHU9J_002601"
     CDS             10473..12014
                     /gene="ppx"
                     /locus_tag="ACHU9J_002601"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1906105.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12053..14296)
                     /gene="pdeF"
                     /locus_tag="ACHU9J_002602"
     CDS             complement(12053..14296)
                     /gene="pdeF"
                     /locus_tag="ACHU9J_002602"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1906106.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3810 a         3691 c         3604 g         3331 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttatgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttg caagctccag cagttttttt cccccttttc tggcatagtt ggacatctgc
     8341 caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag taaaagtggt
     8401 aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt tcaatgaacg
     8461 cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc gtttcctggg
     8521 gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac tgaagcgacg
     8581 catcattatt agcgaagaac aaggctccaa ctctcattcc cgccatttac tgggcaaaat
     8641 tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg agctgctgct
     8701 ggagatggcg cgtaaccaga tcttcctgat taatgaacgc cagctctccg tcaatcaaca
     8761 aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc cgattttaat
     8821 caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc tggcggtgga
     8881 aattatccgt ggcgatacca tccgttacgc gctgctggag atcccatcag ataaagtgcc
     8941 gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc aagccgatga ttcttctgga
     9001 taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt atgacgcgct
     9061 gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc atgagatgga
     9121 agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg ctgagccggt
     9181 gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac gcgaaaaact
     9241 gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt ttaaagactt
     9301 tattaatttc cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc cgcgtttacg
     9361 ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc gcgaacgcga
     9421 tgtgttgctc tattatcctt atcacacctt tgagcatgtg ctggaactgc tgcgtcaggc
     9481 ttcgttcgac ccgagcgtac tggcgattaa aattaacatt taccgtgtgg caaaagattc
     9541 acgtatcatc gactcgatga tccacgctgc gcacaacggc aagaaagtga ccgtggtggt
     9601 tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc gcctgaccga
     9661 agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca aactgttcct
     9721 gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga ccgggaactt
     9781 taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag atgcgcgtat
     9841 caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc cggtgacatt
     9901 tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa tggtagaccg
     9961 cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc taaataacct
    10021 tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg taccggttaa
    10081 tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta gcgacaacat
    10141 tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata tttttgaaaa
    10201 tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca atattgatta
    10261 tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg tgctggacat
    10321 catcgacata ttgttcagcg atacggtcaa agctcgttat atcgataaag aactcagtaa
    10381 tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga tttatgacta
    10441 catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa tcccctcgtc
    10501 cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc atagcccgtg
    10561 tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat ctggcggacg
    10621 gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg aactgtctgt
    10681 cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata gtcggcaccc
    10741 atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa aaggtcattc
    10801 cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt atgggcgtgg
    10861 aacataccca accggagaaa ggtcgcaaac tggttattga tattggcggc ggatctacgg
    10921 aactggtgat tggtgaaaat ttcgaaccga ttctcgttga aagccgccgg atgggttgtg
    10981 tcagctttgc ccagctttac ttccctggcg gggtcatcaa taaagagaat tttcagcgcg
    11041 ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt attcagggct
    11101 ggaacgtggc aatgggcgct tccggtacca taaaagccgc ccatgaagtg ttaatggaaa
    11161 tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta aaagaagttt
    11221 tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag cggaaaacag
    11281 tcttcgttcc tggactggcg attttatgcg gtgtgtttga tgctttagcc atccgtgaac
    11341 tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta cgaaatggaa ggacgtttcc
    11401 gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat cacatcgaca
    11461 gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat gtacgaacag tggcgggaac
    11521 agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc gccatgctgc
    11581 atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct tatattctgc
    11641 aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg acactggtgc
    11701 gctatcaccg taaagcgatt aaactcgacg atctgccgcg ctttaccttg tttaagaaga
    11761 aacagttcct gccactgatt cagctattgc gccttggcgt attactcaac aatcaacgtc
    11821 aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac tggacactgc
    11881 gtttcccgca tgactggttt agtcagaatg cgttggtact gcttgatctg gaaaaggagc
    11941 aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa agtacacctg
    12001 aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt attcaggcac
    12061 tttcgcgaat gggttcgatt tcattcaacg tatcaattaa cggctgcggc ttaccaataa
    12121 gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc tcttcgtttt
    12181 caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa atcgacgcca
    12241 caatctgata atccagacta tttgacacaa tattgcggat aaagctgccg tcaattttaa
    12301 gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg ccgaaatcat
    12361 caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc gcctgcttaa
    12421 cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct tcaatctgat
    12481 atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc gaggttggag
    12541 acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc ataaattgca
    12601 gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg tgcgcgaccg
    12661 gcaagaagct atcggggacg atcagttcat cattctcacc tttcatgcgc agaagaattt
    12721 catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa aaatggttgt
    12781 gttccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat tcgcgttgca
    12841 aatacattgc cccgcgacgc tgcatatttt ccggggcgtt ggtcacgatg gaaagttcgg
    12901 cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag cgcacatagc
    12961 agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa cgaaattgct
    13021 tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc agtcgcagcg
    13081 cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag ggtgacagcc
    13141 agtgagaaag tttttgcttg tattgaatcc gcagcatgat gccatagttc ttaaccagca
    13201 tttccatgcc agggatgcgt aaataacaaa gcgcagacca gggagcatca cgcaacgcgc
    13261 gattcagggc gcgaacattt ggcagatgaa ccaccgggtc catatacgca agccgctgaa
    13321 tgcgtctgac taccgctctc tgacgggttg ccagtacagc catataattg acaataaaag
    13381 agaataccag ataactggag gaggttatgg tcagctgcgt ggtatagcca ggataaatgg
    13441 gaatgtaatt ttgatagctg tggatgctga tcatcaacac gaccgcccag agcagcgaaa
    13501 tcagcttata accatagcgc atcgctcccc acatcatcag gggcagcaat aatgacaagg
    13561 tataattggt gctaaaaatt gtgctttttt catttaacgg catgcataac agcaacaata
    13621 aagcacctaa tgccagtagc cagagcgcga actctttttt ggtgactttg gcatcaacct
    13681 gctgttttaa ttgcgaataa tagctacgta aataaaatgg atttcgcact acccgaatga
    13741 tgaagtagca cagcgggaca ccgatcagat tacccaccag taaggcctga taattgatta
    13801 aagtcccgag gttaaaaggc atgacaccga ccagattttc tctgctcgcc agtaatccta
    13861 caaatgcagc aaactggaaa agtatcagaa acagcgttgc aggaaacaca atctgccaga
    13921 atatacgctg ggaaattaaa cgggtatcac catgtgaaac gttgttacgc cggggagtaa
    13981 agaccctgta accgccccaa cagagaatga taatgataat aaaattagcc gttaatgata
    14041 gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa aatccctaag gcgatccctg
    14101 gcaatgccgc ccagctgaaa aacatcatca tgctgatcat cagtgccaga ggcagataaa
    14161 agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt aatgtgggcg aaaatgggca
    14221 agattaaaga aggcaggaac agcggaagcc cccaccattt atcacgcatt tttatataag
    14281 ttgcattcag tttcatagat gctcagcaga atcccccaca tcctgaagga ggtgtattca
    14341 gacaggcatc ccacctgact tcgaatgatg attattcatc actatagaga gcattgattc
    14401 taagtgtcat atgaaagttc caattgatat atatca
//
LOCUS       DAZPJP010000096        14386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPJP010000096 DAZPJP010000000
VERSION     DAZPJP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.096"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHU9J_002603"
     CDS             complement(<1..80)
                     /locus_tag="ACHU9J_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906107.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGT"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="ACHU9J_002604"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="ACHU9J_002604"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1906108.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHU9J_002605"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHU9J_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1906109.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHU9J_002606"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHU9J_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1906110.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHU9J_002607"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHU9J_002607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1906111.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHU9J_002608"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHU9J_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1906112.1"
                     /translation="MITVALIDDHLIVRSGFARLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHU9J_002609"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHU9J_002609"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1906113.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHU9J_002610"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHU9J_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1906114.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHU9J_002611"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHU9J_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1906115.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="ACHU9J_002612"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="ACHU9J_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1906116.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHU9J_002613"
     CDS             complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHU9J_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1906117.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="ACHU9J_002614"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="ACHU9J_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1906118.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="ACHU9J_002615"
     CDS             complement(11403..11900)
                     /locus_tag="ACHU9J_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1906119.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="ACHU9J_002616"
     CDS             complement(11897..12259)
                     /locus_tag="ACHU9J_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1906120.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="ACHU9J_002617"
     CDS             complement(12249..12596)
                     /locus_tag="ACHU9J_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1906121.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /gene="yidI"
                     /locus_tag="ACHU9J_002618"
     CDS             12705..13154
                     /gene="yidI"
                     /locus_tag="ACHU9J_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1906122.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13201..13359)
                     /locus_tag="ACHU9J_002619"
                     /pseudo
     CDS             complement(13201..13359)
                     /locus_tag="ACHU9J_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13403..>14386)
                     /locus_tag="ACHU9J_002620"
     CDS             complement(13403..>14386)
                     /locus_tag="ACHU9J_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1906123.1"
                     /translation="SQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVK
                     LDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSA
                     SMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKI
                     QRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVRAKS
                     GLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSKF
                     RCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3395 a         3970 c         3728 g         3293 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgctaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 cgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ctccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt gtgactttgt
    13861 gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct
    13921 gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga agtctcgcca
    13981 gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca tctgacagcg
    14041 tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg tgaaccggag
    14101 ttgatacttc actttctgtc tgaatactga tgtaccactt accgcaggac tggctgacag
    14161 tgacattttt cacaacaccc gtgacctgac ggctgttccg gtagcgcatc cagcccagtt
    14221 tcggcagaaa aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata
    14281 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgattctgg aagaagtttt
    14341 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtga
//
LOCUS       DAZPJP010000097        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPJP010000097 DAZPJP010000000
VERSION     DAZPJP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.097"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHU9J_002621"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHU9J_002621"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1906124.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHU9J_002622"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHU9J_002622"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1906125.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHU9J_002623"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHU9J_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1906126.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHU9J_002624"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHU9J_002624"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1906127.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHU9J_002625"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHU9J_002625"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1906128.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHU9J_002626"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHU9J_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1906129.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHU9J_002627"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHU9J_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1906130.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHU9J_002628"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHU9J_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1906131.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHU9J_002629"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHU9J_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1906132.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHU9J_002630"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHU9J_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1906133.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPJP010000098        14211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPJP010000098 DAZPJP010000000
VERSION     DAZPJP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.098"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHU9J_002631"
     CDS             <1..153
                     /locus_tag="ACHU9J_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1906134.1"
                     /translation="RRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEGTSGSNRYGND
                     PKFGSN"
     gene            complement(204..1096)
                     /gene="yhaJ"
                     /locus_tag="ACHU9J_002632"
                     /pseudo
     CDS             complement(204..1096)
                     /gene="yhaJ"
                     /locus_tag="ACHU9J_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1201..1902
                     /locus_tag="ACHU9J_002633"
     CDS             1201..1902
                     /locus_tag="ACHU9J_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1906135.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1925..2089
                     /gene="yhaL"
                     /locus_tag="ACHU9J_002634"
     CDS             1925..2089
                     /gene="yhaL"
                     /locus_tag="ACHU9J_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1906136.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2223..3533)
                     /gene="cyuA"
                     /locus_tag="ACHU9J_002635"
     CDS             complement(2223..3533)
                     /gene="cyuA"
                     /locus_tag="ACHU9J_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1906137.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3561..4892)
                     /locus_tag="ACHU9J_002636"
     CDS             complement(3561..4892)
                     /locus_tag="ACHU9J_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1906138.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5167..6531)
                     /gene="tdcG"
                     /locus_tag="ACHU9J_002637"
     CDS             complement(5167..6531)
                     /gene="tdcG"
                     /locus_tag="ACHU9J_002637"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1906139.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6603..6992)
                     /locus_tag="ACHU9J_002638"
     CDS             complement(6603..6992)
                     /locus_tag="ACHU9J_002638"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1906140.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7006..9300)
                     /gene="tdcE"
                     /locus_tag="ACHU9J_002639"
     CDS             complement(7006..9300)
                     /gene="tdcE"
                     /locus_tag="ACHU9J_002639"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1906141.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9334..10542)
                     /gene="tdcD"
                     /locus_tag="ACHU9J_002640"
     CDS             complement(9334..10542)
                     /gene="tdcD"
                     /locus_tag="ACHU9J_002640"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1906142.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10568..11899)
                     /gene="tdcC"
                     /locus_tag="ACHU9J_002641"
     CDS             complement(10568..11899)
                     /gene="tdcC"
                     /locus_tag="ACHU9J_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1906143.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11921..12910)
                     /gene="tdcB"
                     /locus_tag="ACHU9J_002642"
     CDS             complement(11921..12910)
                     /gene="tdcB"
                     /locus_tag="ACHU9J_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1906144.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13009..13947)
                     /gene="tdcA"
                     /locus_tag="ACHU9J_002643"
     CDS             complement(13009..13947)
                     /gene="tdcA"
                     /locus_tag="ACHU9J_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1906145.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3699 a         3813 c         3319 g         3380 t
ORIGIN      
        1 cgtcgcttac acgataccga ccgttcaggt gcctgggcct tgctcttttt tgttccgttt
       61 attggctggc tcgtgcttct ggtttttttc tgcacagaag gtacttctgg cagcaatcgt
      121 tacggaaacg atccgaagtt tggttcaaat taatcttaga attggggcga tatttcgccc
      181 ctttttatta acaataataa atattatttt ccgctaaaaa gtttgggaat ttcccgcaga
      241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctca
      301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
      361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
      421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta cggtcaacac cgggcgctca
      481 cgagcggtat ccgctaccgc aattccacga tatttcacgc gcgtcacttc agataacggt
      541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
      601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacaata
      661 tccgcccgcc cctgctccag tcgttcctac gcccccgcca gcacttcggt gatgattgcc
      721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
      781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag ggcttccgca
      841 tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
      901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
      961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgcc
     1021 gccgccgcaa aactgccccg gcgatcgcat ccataacccg tagtgcttcc agcgttaatg
     1081 ccctttcttt ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct
     1141 ggctaacaat gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt
     1201 atgattacta cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc
     1261 cggtatactt tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg
     1321 cgtgtgctta accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc
     1381 aaagtcgata ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc
     1441 aatcatgttc aggccagcgc cggtgaggcg ttgctgctct ctacccagcc gggtgtcagc
     1501 tacagcgaac acaatctcag caaagacaaa ccgttaacac gaatgcagct ttggctggac
     1561 gcctgcccgc agcgagagaa tccgctgatt caaaaactgg cgcttaatat gggcaagcag
     1621 caattaatcg cctcgccaga gggtacgatg ggaagcctgc aattacgcca gcaagtgtgg
     1681 ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc
     1741 gcgtatttgc aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg
     1801 gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc
     1861 gattccccac tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat
     1921 gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga
     1981 acctgctcgc accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagccat
     2041 cgtcgcggat gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc
     2101 cggatgcggc gaaaacgcct tatccggcct acaaaatcca acaacattaa cgaattaaac
     2161 aacccgtagg ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc
     2221 gtttatctgg ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa
     2281 tggcttgcta acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct
     2341 tcattgccgg tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa
     2401 gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg
     2461 acatcgccga tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc
     2521 catgccatcc cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa
     2581 cgcggtaact ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc
     2641 agtcgttcat catccgctcc gaagtgttct gctacaacca ccacaggcat tgttgcggtg
     2701 atcccctggt tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc
     2761 gcatcggatg ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa
     2821 ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa
     2881 ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca
     2941 aacgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc
     3001 agcggagact cttgctcgcc ctctgccaca cacgcctgct gggtaaacac cacaccattg
     3061 tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc
     3121 tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta
     3181 acggagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg
     3241 tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca
     3301 atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga
     3361 tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc
     3421 gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc
     3481 tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa
     3541 accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa
     3601 ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg
     3661 taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct acagatagag
     3721 gtgaagctca acaccggtgc gttcagtacg atagcgctcc aggccagcaa aatggcgaaa
     3781 atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc
     3841 aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat
     3901 gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg
     3961 tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt
     4021 accgcttcgt catgccccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca
     4081 aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaata
     4141 gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat aaacaaaatc
     4201 gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt
     4261 agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag cgccgcgacc
     4321 accagcagct tggtcagcac catgccggtc gaaattttga ataacaattt ctcgccgcgt
     4381 gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc
     4441 aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg
     4501 gaatagacga acatccaaat caccagcatt acgaaataga acgcacctaa caggatgccc
     4561 cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct
     4621 gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat
     4681 gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc
     4741 gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg
     4801 atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct
     4861 gcaatgacgc ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct
     4921 tattattttt ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt
     4981 attagaataa agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttga
     5041 aataacggag atggataaga attttctact aaattaatcg caagagaata ttcattctct
     5101 atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca
     5161 gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt
     5221 atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg
     5281 cggtgccgag gtgcggcgca tcgccatcca cgcggcgttt actgctttca cggcattaat
     5341 ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc
     5401 aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag
     5461 tagttcagtt aaccctgccg ccgccattga acaggccacg ccaatctccc cctgacagcc
     5521 gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc
     5581 cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc
     5641 cagcacggcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc
     5701 agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat
     5761 cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgacg
     5821 cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc
     5881 atgcatcact tgccagatac gggcaaatcc tgcgtcaatt tccgctttgc tgcgcagcgc
     5941 cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag
     6001 cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc
     6061 gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag
     6121 cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc
     6181 gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac
     6241 tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac
     6301 atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt
     6361 gcccgtcagt gacaacgacc catacagatc gaccacaata tggctcgtcg cggttaataa
     6421 gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg
     6481 cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc
     6541 ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg
     6601 ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc
     6661 gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta
     6721 tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg
     6781 atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa
     6841 cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc
     6901 ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct
     6961 ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt
     7021 aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc
     7081 gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag
     7141 cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc
     7201 gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt
     7261 gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa
     7321 cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat
     7381 cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt
     7441 tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt
     7501 gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat
     7561 gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc
     7621 caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag
     7681 ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg
     7741 atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat
     7801 attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat
     7861 cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat
     7921 cttcagcttc tcgtccaccc cgccgttaat tgcgtacagc agtgttttcg ccaggttagc
     7981 gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc aacaagcaat
     8041 tgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcgttttcat actgcaagga
     8101 agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga
     8161 ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata
     8221 gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc
     8281 cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggcgtac gcaagaagcg
     8341 caccatacgg atcttcataa tgaagtgatc gatcagttcc tgtgcctgct gctcattgag
     8401 tacgcctgct ttaaagtcgc gctcaatgta gatatcgagg aacgacgccg tgcgccccag
     8461 cgacatcgcg ccgccgttct gtgatttcac tgccgccaga taagcgaagt agagccactg
     8521 caccgcttcc tgcgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat
     8581 ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt
     8641 ggcttccaga tcttcgcctt tttccagacg ggactggaga tcggcaaatt gcagttcgcg
     8701 ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg
     8761 cccacggcca tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat
     8821 atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag
     8881 atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt
     8941 aatgccaccg aacggatgca gcgcgcgctt taatggcgca tccgtttgca gaccaacaat
     9001 tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt
     9061 ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacttt
     9121 ttcccacaat tccgtggtgg caggcgtcgc ttcggcgagg aaagattcat cgccttcata
     9181 cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgtttttcc agtccgtacc
     9241 tttaaagcca agccatgcgt cggcgtacag cttatcgctg gtatcaatat ctatcttcat
     9301 gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac
     9361 tttgcctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacggc
     9421 acaaatgaca cgcgcatttt cactggaaac aattcgctct ccaaaagagt tagagcgatt
     9481 attcatttct gtatcaatca ctacgcctaa tacagccaaa tgttccatga ccagacgacg
     9541 aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca ggcgacgtaa
     9601 tgaagctgcg tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag
     9661 ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag
     9721 accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct
     9781 ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt
     9841 tcccatcatc aagccttcca gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt
     9901 gcgaaccgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc
     9961 ttccgccaga ttcagcagag aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg
    10021 gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata
    10081 agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc
    10141 cggaaataat tgctgcgccg attcaatacc acttaaattg gcgtaattat gcaggggtgc
    10201 cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt
    10261 aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacactgt catttaaatt
    10321 ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag
    10381 cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc
    10441 aatacctgac attaatactt cacagtcgct ggcatcgagc acggaaaact taatcgaaga
    10501 cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag
    10561 ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa
    10621 caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat
    10681 acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt
    10741 cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc
    10801 tgatagtgtt cagtttaccc agcgacactt tagttttgtc gcctttataa ccaaacttca
    10861 ggaccaggcc attcagacct tccagcgttc ccagatagtg accgaagaaa gatttgaaga
    10921 tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg
    10981 taccagtcat ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt
    11041 tggcttccgc catgttggcc ggagacagcg taaataggca gctaaaggca aagaacatca
    11101 ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga
    11161 agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga
    11221 ttggcgagaa attaaaggag aaaaccatga tggaaatccc cagccagacg gtgatcagga
    11281 taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt
    11341 tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca
    11401 ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca
    11461 gcagcaggaa cagcgccaca aagccgcgat tcagcggtgc aaagccgagc tggttttccc
    11521 agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg
    11581 cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg
    11641 tttccgtaat gttgccggaa gggttagagc cagaaagaca tagacgcgcc agcgcccggt
    11701 ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc
    11761 caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc
    11821 ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa
    11881 tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg
    11941 tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc
    12001 tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg
    12061 cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt
    12121 cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac
    12181 ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt
    12241 ctccggagtg gaaagaagcc gccatgccgt gaacgttttc agactgtacg ccaataacac
    12301 gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccgccaccac
    12361 caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg
    12421 ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata cggccttcca
    12481 tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga
    12541 cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca
    12601 ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc
    12661 ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat
    12721 taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata
    12781 tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc
    12841 gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaatcggc agatcgtatg
    12901 taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac
    12961 ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc
    13021 tattaattgc cttcgtctac acccattata agatgaatac tctttggcta attccaccaa
    13081 aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac
    13141 aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat
    13201 atcacaagga attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac
    13261 tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag
    13321 ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc
    13381 cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc
    13441 gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc
    13501 aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat
    13561 agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt
    13621 aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc
    13681 gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa
    13741 caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc
    13801 aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc
    13861 ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg
    13921 cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat
    13981 gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt aactttcaat
    14041 actttccagg gtatcgtcat tacaattaaa tacctttaaa tatcaacaag ttaaagcata
    14101 aaaatcagca taaaccctga tttttgtcaa aaaaaatgac ggggataacc atattttatg
    14161 gcgataacat cattcgttat gtggttaata caaaaaaggg gctgagacca t
//
LOCUS       DAZPJP010000099        14146 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPJP010000099 DAZPJP010000000
VERSION     DAZPJP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.099"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHU9J_002644"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHU9J_002644"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1906146.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHU9J_002645"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHU9J_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1906147.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHU9J_002646"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHU9J_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1906148.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHU9J_002647"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHU9J_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1906149.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHU9J_002648"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHU9J_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1906150.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHU9J_002649"
     CDS             5280..5738
                     /locus_tag="ACHU9J_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1906151.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHU9J_002650"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHU9J_002650"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1906152.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHU9J_002651"
     tRNA            6790..6874
                     /locus_tag="ACHU9J_002651"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHU9J_002652"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHU9J_002652"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHU9J_002653"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHU9J_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1906153.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHU9J_002654"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHU9J_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1906154.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHU9J_002655"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHU9J_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1906155.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHU9J_002656"
     CDS             complement(10156..13899)
                     /locus_tag="ACHU9J_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1906156.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3504 a         3736 c         3381 g         3525 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtatt
//
LOCUS       DAZPJP010000100        14062 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPJP010000100 DAZPJP010000000
VERSION     DAZPJP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.100"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHU9J_002657"
     CDS             132..1151
                     /locus_tag="ACHU9J_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1906157.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            complement(1425..1634)
                     /locus_tag="ACHU9J_002658"
                     /pseudo
     CDS             complement(1425..1634)
                     /locus_tag="ACHU9J_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1836..2480)
                     /gene="cheZ"
                     /locus_tag="ACHU9J_002659"
     CDS             complement(1836..2480)
                     /gene="cheZ"
                     /locus_tag="ACHU9J_002659"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1906158.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(2491..2880)
                     /gene="cheY"
                     /locus_tag="ACHU9J_002660"
     CDS             complement(2491..2880)
                     /gene="cheY"
                     /locus_tag="ACHU9J_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1906159.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(2895..3944)
                     /gene="cheB"
                     /locus_tag="ACHU9J_002661"
     CDS             complement(2895..3944)
                     /gene="cheB"
                     /locus_tag="ACHU9J_002661"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1906160.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(3947..4807)
                     /gene="cheR"
                     /locus_tag="ACHU9J_002662"
     CDS             complement(3947..4807)
                     /gene="cheR"
                     /locus_tag="ACHU9J_002662"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1906161.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(4826..6427)
                     /gene="tap"
                     /locus_tag="ACHU9J_002663"
     CDS             complement(4826..6427)
                     /gene="tap"
                     /locus_tag="ACHU9J_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1906162.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(6473..8134)
                     /gene="tar"
                     /locus_tag="ACHU9J_002664"
     CDS             complement(6473..8134)
                     /gene="tar"
                     /locus_tag="ACHU9J_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1906163.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(8279..8782)
                     /gene="cheW"
                     /locus_tag="ACHU9J_002665"
     CDS             complement(8279..8782)
                     /gene="cheW"
                     /locus_tag="ACHU9J_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1906164.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     NGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(8803..10767)
                     /gene="cheA"
                     /locus_tag="ACHU9J_002666"
     CDS             complement(8803..10767)
                     /gene="cheA"
                     /locus_tag="ACHU9J_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1906165.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(10772..11698)
                     /gene="motB"
                     /locus_tag="ACHU9J_002667"
     CDS             complement(10772..11698)
                     /gene="motB"
                     /locus_tag="ACHU9J_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1906166.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(11695..12582)
                     /gene="motA"
                     /locus_tag="ACHU9J_002668"
     CDS             complement(11695..12582)
                     /gene="motA"
                     /locus_tag="ACHU9J_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1906167.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(12708..13286)
                     /gene="flhC"
                     /locus_tag="ACHU9J_002669"
     CDS             complement(12708..13286)
                     /gene="flhC"
                     /locus_tag="ACHU9J_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1906168.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(13289..13639)
                     /gene="flhD"
                     /locus_tag="ACHU9J_002670"
     CDS             complement(13289..13639)
                     /gene="flhD"
                     /locus_tag="ACHU9J_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1906169.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         3334 a         3814 c         3464 g         3450 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aagtccgccg tgaaaataag gggtgagtcg gcgaattaca
     1321 taggggctcg cagcgttacg gctgcaataa agccggatat aaagctgcaa cctacccgtc
     1381 atgtcaaaac aatggatgcc ttgcaaacgg gatgcgttca tataaatact gtgatcaaaa
     1441 tgcagcccag cggagagcat gcccgacaat cgacgggcca gcgacacacc gctaaaccag
     1501 ataacacaca cgcccactaa caaaatcagc aacgaggtca gttcacggga acgcgggatt
     1561 tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg gggcttctgt tttgtcgtcg
     1621 ctctcgtcag acacgtcgcc aatcctggaa agagtattaa gcttaaatga tgccagagcg
     1681 caaagcgttc aatggtttga gtaaggggca aaacaggcgg gatttagggc ttttgctgcc
     1741 acacatcaag catagtgtgc gtttgtcgga tgcggcgtca tcgccttatc agaccgcctg
     1801 atatgacgtg gtcacgccac atcaggcaat acaaatcaaa atccaagact atccaacaaa
     1861 tcgtccacct gatcctgact ggctaccacg ccgactttgc tggtatcgac ctgaggtcca
     1921 ttaagcaaac tctggttttc acgttttgga cgcgactcct gttccgggat gttttccaac
     1981 agcaccatca gcaactggcg ttcgatctcc tgaatgacat ccatcatccg cttaatgacc
     2041 agcccggtga gatcctgaaa atcctgcgcc atcatgattt ccagcagttg cgcgttagta
     2101 aagctggtat gcgcgggtac atctgccaga aattgtcgtg tatctgttac cagttcacgg
     2161 gcatcggcaa ggtcaatcgg atcggcaaac cagtcatccc aacgttgggt taacgctttt
     2221 gctgatttct ccatttgatc ctgatgcggt tgtgacgcct caacactgtt cagcgcccgc
     2281 tccgcagcct gggcggtcat ctgcacaaca tagtacaaac gatcgcgcgc atcggggatg
     2341 gcttccgccg cttcggcaat ggcctgatcc agccccagtt cccgcaaact gtcgcgcagc
     2401 atacgcgtca ggctgccgat gcgcgcaatg atatcgccag ctgaatgctc gtcagcaggt
     2461 ttgattgatg gttgcatcat agtcgcatcc tcacatgccc agtttctcaa agattttgtt
     2521 gagtttttcc tccagcgtcg cggcggtaaa tggcttcacc acatagccac tggcccccgc
     2581 ttgcgccgca gcaatgatgt tctctttctt cgcttctgca gtcaccatta acactggcaa
     2641 tgccgacatc gcgccatccg cacgaattgt tttcagcaat tccaggccat ccatattggg
     2701 catgttccag tcggagataa caaatccata accgcctgcc tgcaacttat tgagagcgtc
     2761 gacgccatct tccgcttcct caacattatt gaatcccagc tctttcagca ggttacgcac
     2821 tatgcgtcgc atggtggaaa agtcatccac aaccaaaaat ttaagttctt tatccgccat
     2881 ttcacactcc tgatttaaat acgtatcgcc tgtccggcac taatttttgc caacatttgc
     2941 tggcttacct ggctaagatc gaccacttcg cagacaccac ccatattgat ggcctcgcgc
     3001 ggcatgccga acaccacgca acttgcttcg ttttgcgcaa gggtccatgc ccccgcctga
     3061 cgcatcgcca acattcccgc cgcgccgtcg ttgcccatac cggtcaggat caccccaacc
     3121 gcattacgcc ccgcctgttt ggcgacagaa tggaacaaca catctaccga aggccgatga
     3181 cggttaaccg ccgggccgtc gtgaattttg atttgataat ttgcgccact acgcgccagt
     3241 tccatatgcc gatcgcccgg cgcaatatag gcatgccccg gcaagacacg ttctccgtct
     3301 tcggcttctt taaccccgat ctggcaaagc ttattaagtc tgtcggcaaa agagcgggtg
     3361 aaaccgggcg gcatatgttg ggtaattaac aatgccgggc tggaaagcgg caacggttgc
     3421 agtacgtgac gaattgcctc agttccaccc gttgaagcac caatcgcaat cagtttttca
     3481 gaactcaaca acggacccgc cttcagcgtt gtcggtgctg acaatggctt atgtgctgca
     3541 aggctcgcct ttgctgccgt acgcaccttt tcagcaatca tttcgctata cgccagcatt
     3601 ccttcgcgaa tacccagttg cggtttggtg acaaaatcta ttgcccccag ctccagcgcg
     3661 cgcagcgtga cttctgatcc tttgccggtc aaggaagaaa ccatcacaac gggcattgga
     3721 cgcaaacgca ttaatttttc gaggaaatcc agtccgtcca tccgcggcat ttcaacatcc
     3781 agcgtcagca catcgggatt gaatttctta atcaagtcac gcgcgaccag cggatcaggc
     3841 gcggtcgcca ccatttccat gtcgctatgg ctgttgatga tttctgtcat gatctggcgc
     3901 atcagtgccg aatcatcgac agataacacc ctgattttgc tcatcgttaa tccttactta
     3961 gcgcatacac cgtctgacca cgcagcgtga agcggcgctc aaggtggcta aagttttcag
     4021 agtgacccgc aaacagcaat ccgtcgggtt taaggagcgg aacaaagcgg cgcaaaatct
     4081 cctgctgggt attttgatcg aagtagatca tgacgttacg acagaagatc gcatcaaacg
     4141 gccccggcac ggtgtactgt ttcgccagta gattcagcgg ggcaaaatca acatagttcg
     4201 ccagctcctg acgcacgcgt accagccctt catgcggtcc cgtccctcgc atgaaatacc
     4261 gttgcagttg ctgcggcgtc aggtttttca actcttcatg gcgataaata ccgcttctgg
     4321 ctttttccag cacttcggtg tcgatatcac tggcaaacac tttccagcgt ccgggcgcgg
     4381 tgcccaatgt gtcagccagc gtcatcgcaa tgctgtacgg ctcttcgccg gtcgaagccg
     4441 ccgcgctcca tacgcgatac tcgccagaac ggcgacgtgc gtgatccgcg agcagaggga
     4501 aatggtgtgc ctcacggaaa aatgccgtca gattcgtggt cagcgaattg ataaacgcct
     4561 gccactcacc gctgtgctga ttagattcca gcaagttcag ataatgaccg aaatccgtca
     4621 gtcccagcga acgcaaacga cgaaccagtc ggttgtaaac catgtcgcgt ttatggtcag
     4681 ccagaacgat cccggctcgt tgatagatca attgacttat ccgccgaaaa tgcgcgtcgg
     4741 aaagcgccag gcgctcggtc atctgtaaca ataaagacgt ttgcccacag ggcagagatg
     4801 aagtcatagc gccttctcaa tcacttcagg ataccactgg cgcaatttgt aactgcgccg
     4861 actcatgtcg tgctacttca tgttcttcaa gggtaaatac cgccacacgc gacgaaagat
     4921 ggtcggcctg attcgccagt tgttccgttg ccaccgccgc ttcttctacc agcgaggcgt
     4981 tctgctgagt cacctgatcc atctggctga cagcctgtgc aacctgctca atcccccgct
     5041 gttgttcttc cgacgcagag gcaatttctc ccataatgtc gttcacgcgg gtcaccgaac
     5101 tgacaatatc gatcatggtc gcggcggcgt tattcaccag tttcgaaccc tgctggacac
     5161 gattgactga ctcttcgatc agccctttga tctcttttgc tgcctgcgcg ctacggctgg
     5221 caagattgcg cacttcacct gccactaccg caaaaccacg cccctgctct ccggcgcgag
     5281 ccgcttccac tgccgcattc agggccagaa tattggtctg gaaagcaatt ccgtcgataa
     5341 cgctgataat gtcgccaatt ttttgcgagc tggtggcgat ctcctgcatg gtgtgagtca
     5401 tggtactgac ctgaacaccg cccgcctgcg ccgttgtcgc ggcatttttt gccagttccg
     5461 acgcctgtcg tgcgttatcg gcgttttgcc ctaccgtggc ggttaattgc tccatactgg
     5521 cggccgtttg tgccagcgat gccgcctgct gttcggtacg acttgagaga tcgttattgc
     5581 ctgcgacaat ctccgcgata ccaatgtgca tctcctggct tcccttacgc acatcactta
     5641 ccgtcccacg caaagcctgc tgcatggtct tcagactggc aaaaatggcg gtgatctcat
     5701 tacgaccata taccgcaatc ggacgcgcca gattacccgc agcaatgctg tcaaaatggc
     5761 taccgataat ggccagtggt tgaacaatca tcttgcgcgt ccaccacagc gcactgctga
     5821 tgtagatcgc cgcaacaata atcatgctga taaacaccag tgtcgaaatc tgatagttac
     5881 gctggctttg cgcactggcg gcttccagca catggttgat ctccagctgc caggcctcaa
     5941 agttaacgtc aaacgcattc tgtgatccct gcaccggcgc agtgaggaaa tccgaaagtt
     6001 gattactttc aagccaggtc gcctgatgtt cgagatcgtt gtgccagcgg gcaaaacttt
     6061 tctccgtctc tttttgcaat gccctgacgt gctcgttgcc cgcagtcatc gccataaaac
     6121 ttttaaacag cgtggtggat tgcgtcagac tggcgcgcgc cgtcgtcatc aacgttttaa
     6181 tgtcatccgc cggatagcta agtgccgtca gagtgcccgc tttgttcagc gcggtactgg
     6241 cctgtaacat taccgcccga gtttgcgcta atgccgcacg ttgctgattg ctctgctcga
     6301 cctgattcag tcgttgcaaa tcgtcgcgaa atgcccaaaa agacatgccg ttactgccaa
     6361 tctgcaagat cccgcagaga atcaaaatta aaaacagcgt ggtcgaaatt cgaatacgat
     6421 taaacatcaa cgctccccat caggcggcaa tgaccgcgtt agtaaatact cgtcaaaatg
     6481 tttcccagtt tggatcttgt tcagcaattc gcagtcgtgg ctgtgccggt ggttgctcac
     6541 tggcaggacg ggatggtgtt tgcggtttat tggtgagtgg gctggctgcc agacggaacg
     6601 cggaaaccgc ttgcgttaaa cgactcgcct gttcttccag cgcagcggcg gcggcagctg
     6661 attcctgcac cagcgatgcg ttctgttgcg tgacgcgatc catttccgaa accgccaatg
     6721 cgacttgatc gatgccacgg ctctgttcat ccgatgccga tgcaatctcg cccataatgt
     6781 cagtcacgcg agtgacagca ttgacgatat tgttcattgt ttccccggcg ctttcgacca
     6841 gcaccgaacc ggtatcaacg cgtgcgacgg agtcttcaat gagggctttg atctcttttg
     6901 ccgcctgggc gctgcgactg gcaagattac gcacttcacc cgccaccacg gcaaaaccac
     6961 ggccctgttc acccgcacgc gcggcttcaa ccgcggcatt cagcgcgagg atattagtct
     7021 ggaaggcaat accgtcgata acgctgataa tgtcggcaat tttcttcgaa ctatcggcga
     7081 tctcatgcat cgttttcact acgccatcca ccactttgcc gccgtgctgg gcggtgtcgg
     7141 aggcactttg cgccagttgc gaggcctggc gggcgttatc ggcgttttgc ttcactgtcg
     7201 cggtgagctg ctccatgctg gcggcagttt cttccagcgc ggatgcctgc tgttcagtac
     7261 gggaggaaag atcggtgttg cccgccgcaa tttcacgggt accggcatag atggcatctg
     7321 aaccttcgcg gacatgagtg acggtgtcag tcaaagagcg ttgcatatgt gaaacgctct
     7381 gcgccaggtc gcccatttca ctgcgcccgt caatggtcag ggtattcgct aggttaccac
     7441 cggcgatttc gcgaatgtga gcaataattt ttgccagcgg agtaagcaac atacggcgaa
     7501 tgccgtacca cgccaccagc agaatcaata ccaccaccag cgcgataacc gccagttgcc
     7561 actgggcaaa tcggtaatca tctgcgttgt cagtgacgat atcgcgatac agtttttcac
     7621 tgctgagggc gtactgagca aacgcttcgc ccattgcatt ttgcattccc tgggttggct
     7681 gagcgaaata agctccagta ttgccataat ctagataatc aatcagttca gttaacgctg
     7741 tgtaatagtt tttatatttt tcatcaatat tacgactggt agcgaccatt tcaggtaacg
     7801 gtgccatgct tttgaatttt ttataatgcg tcgctgcctg cgccaatgtt ttcctggcgc
     7861 tatcgagcaa ttcaactttg gcgttacttt gttgattgga ggaatccatc atcatccgca
     7921 ccgctgaacg actcaggtta atgcgcgttt gcagcattaa atcccaggtt gacgtcagct
     7981 cgccctgctg ttcccgcaat tgattggaaa ccacaaagct cttctggcta tggtgaaggg
     8041 aagaaaaaaa cagactgccg gaaataagct gtaacagtgc gaataccccc agcaccatta
     8101 ccaacagcgt gactacgcgg atacggttaa tcataaggca ccttcctgaa aacaagttga
     8161 tctcgttatc ggcaagaagg ggggaaactt tattgctgat gccacccgcc gcgaaattga
     8221 aataaaaaac ccgatgcgca gatcatcggg ttcatttcaa ttgaggaaat cgggagaatt
     8281 acgccacttc tgacgccgcg ctatctaaca gcgccatctc ttcgctgttc agcagttttt
     8341 cgatgttcac cagaatcaac atacggtcgc ccagtgcgcc cagtccagtg agatattctg
     8401 ttgaaagcgt cacggcaaac tctggtgccg gacgaatttg ctccgccgtc aatgaaagca
     8461 cgtctgaaac gccgttaacc acgatgccga ccacccgctg tccgagattc aggacgataa
     8521 ctaccgtgtt gtcgttatag tccacatcca cctggctgaa cttaattcgt aagtcaacaa
     8581 tcggcacaat aacgccgcgc agattcgtga cgcctttgat aaacgctggc gtgttcgcaa
     8641 tccgtgttac ctgatcgtag ccacggatct cctgcacttt caggatatca ataccgtact
     8701 cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg gccagctttg
     8761 ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg cggtgttcgc
     8821 catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct
     8881 gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt tactttcaag
     8941 gtttttaacc acaacctggt gttgaccaat taattgatcc accagcaagg cgtagcggcg
     9001 accgccactt tgtaagatca caacaattcc ctgggtggct tcggttttcg cgcccgcgac
     9061 gttgaacact ttccacagtt cgacgatggg cagatattca ccccgcactt ccagcacccg
     9121 ctcgccgccg gccagtggat ggagatcggc ttcacggggt tgcagtgatt ccataacagc
     9181 attcagcggc agaatgaaaa cttcatcccc aacgcgtacg gacatgccgt cgaggatggc
     9241 cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagtc ccctgcttcg actggatttc
     9301 gacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga cgccgcgccc
     9361 ggagacgtcg gtgacctgct ctgccatgga gaagcctggt gcaaatatca gcatcgcgac
     9421 ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc gaggccgctt ttgccagaat
     9481 tcgctcacgg tttagccccg ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg
     9541 atgttcggca gacagaatta aatttccgac gctgttttta cctgcggcga gccgtttttc
     9601 tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat
     9661 gcgttctatc aggcttttgt cgagttcagt agaactgccc accagcgtca gttctacctg
     9721 cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa aaacatattc
     9781 catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa
     9841 ctgccccatg ctggttatca aatcaccatg attaaccggg tccagttcgc tggaacgctg
     9901 ggcaagcatg gactgggtga taaccagctc gccgacgagg ttaattaatt gatcaacctt
     9961 ttctaccgct acacggatgc tggtggattc attgctgcgc gtcgtttttt cccgctccac
    10021 gcggccggtt ggcgcttgtt cggctgccag tttaagcact ggtggggtgg atatttttgg
    10081 cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac agagtaccgc
    10141 tgtgatgtca tcttcggcga tgtcgcccgg taatattgcc gagagcgaat ccgccccttt
    10201 caccacgtca gttaacgttg tcagatgtcc cagttcttct tccagcaggt cgacttcccc
    10261 ggccttcagg cgcgaaagga taattcgtcg cggcgactga ctgcgactct gctcatcttg
    10321 cggttcactt ttggcaacca cacttaatcg ggtcactgcg gatggcgttt cgcctttcgc
    10381 ttctaatgcc agttgacgca aggcctggca gatataatcg aagctggcgg catccggctc
    10441 ttgcgactgt ttataagcgt cgagctgttc ttgcatgatg tccttcgttt ccaaaaacag
    10501 attgataatg tcggtgttga gttgcatctc acctcgtctg gcttcatcga gcaggttttc
    10561 catcagatgc gtggtttcct gcaaaacgct gaagccaaaa gttcctgccc ctcctttgat
    10621 cgagtgggca gcccgaaaga tggcattcaa ttgttcggca tctggcgctt ccggctgcaa
    10681 aaccagcaga tgctgctcca tgtcagccaa cagttcgtcc gcttcatcaa aaaatgtctg
    10741 ataaaaatcg cttatatcca tgctcacgct gtcacctcgg ttcggctgat ggcattgtgg
    10801 gaacactgac ctgtggtgca acctcaggtt tttccagggc gcttactggc tcattctggc
    10861 tttcggcgtt ttcatgcaaa atggcctgtt cggcttgttt gttcagtacc agcaggctga
    10921 tgcgacggtt gacggcatca tcaggtccgc gatcgcttaa gcgcatcgtt gccgccatgc
    10981 cgacgacacg taacactttg ccgctatcca accctccgac catcagttcg cggcgggatg
    11041 cattggcccg atcggcagaa agctcccagt tgctatatcc tttctcaccg ctggcgtagg
    11101 ggaaatcatc ggtatgacct gaaaggctaa tacggttggg aataccgttc agtacaggcg
    11161 caatggcacg cagaatgtcg cgcatatagg gttcgacatc ggcactgccg gttctaaaca
    11221 tcgggcgatt ctggctatcg atgatctgaa tacgtagacc ttcctggacc agatcgattt
    11281 tgagatgggg acgtaacgcc cgcagtttcg gatcggactc tatcaactgg tcgagatcac
    11341 cccgcaattt ccgcaatcga ctttgctcca tgcgtttttt cagatcttcg atgttcggct
    11401 gcttattcac ttccccctgg ctttgggtgt aatcatcacc accgccggga attgggcttt
    11461 cactattaga aatgcgatcg ccgcccgtaa ccgcagtcgc cagtggagtc cggaagtact
    11521 ccgcaatctg aatcagctct tttgggctgg agatggagat cagccacatc accagaaaaa
    11581 aggccatcat cgcagtcata aagtcggcat aagcaatctt ccacgatcca tgtgctgccc
    11641 cgtggctttt ggctttgcgt cgtttgacga caataatcgg atgcgcttga ttcttcatgc
    11701 ttcctcggtt gtcgtctgtt gttgcggatt tttcaccgca cgcacatgct cttccagttc
    11761 aataaacgac ggacgttcgc tggaatagag cgttttgcga ccaaactcaa cggcgatagg
    11821 cggtgcgtaa ccgttcagat tagaaagcag agtgactttg acgcactgca tcattttgct
    11881 ggtttcggcg cttttctgac gtaaaacact cgctaatggg gaaataaatc cgtaagccaa
    11941 taaaatgccg aggaaagtcc ccaccatcgc atgtgcgata agcgcaccca gctcggcggc
    12001 aggacgatcg gctgaaccta acgcgtgaac gccccccatt acagccgcga caataccaaa
    12061 cgccggaagt gagtccccga ccagcgccag actgttcgcc gggacttctg cctcgctttc
    12121 gtgcgtctca atctcttcat ccatcagggc ttcgatttcg aaggtgttca tatgaccgct
    12181 gataatcaga cgcagataat cgacgataaa atcaagcatg acgctatccg cgaggatgcg
    12241 tgggtagctg gcgaagatct cgctctcacg gggattttca atatcacgtt ccagcgaaaa
    12301 catccccatc tgccgcgatt tcgccatcaa ccgataaagc agagccagca gatccatata
    12361 cattgctttg gtgtatttgg agcgacgaaa cagcaacggc agcgccttca gcgtgccttt
    12421 aatcgctttg ccattattgc cgacgataaa cgacccaatc cctgcaccgg caataatcac
    12481 cagttcagcg ggttgataga gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa
    12541 aactgtaccg agaacaacca ggtaacctaa taagataagc acgacatcat ccttccactg
    12601 ttgaccatga caggatgttc agtcgtcagg cgttaacgcg cgattggggc aaaaaaagca
    12661 gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt
    12721 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    12781 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    12841 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    12901 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    12961 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    13021 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    13081 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    13141 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    13201 agcatctgca aacgagcgcc cagggtaatc aattccattg ccagctgaat atcccgcgct
    13261 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    13321 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    13381 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    13441 agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
    13501 ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
    13561 gaacaatcaa acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
    13621 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt
    13681 caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt
    13741 tgcgataagc tgcaataagc agaaccacct ttttggttta atatgtcctt acaaatagaa
    13801 atgggtcttt acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac
    13861 acgcacatac aacggagggg ggctgcgatt ttcaataatg tgtgatgcag atcacacaaa
    13921 acactcaatt acttaacata aatgtataag tcatactttt gttttgggtg tatttcaata
    13981 tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa acaggaataa tcgaaatggg
    14041 atgttgcgca cagttaaaat aa
//
LOCUS       DAZPJP010000101        14006 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPJP010000101 DAZPJP010000000
VERSION     DAZPJP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.101"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..263)
                     /locus_tag="ACHU9J_002671"
     CDS             complement(<1..263)
                     /locus_tag="ACHU9J_002671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906170.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQL"
     gene            298..1023
                     /locus_tag="ACHU9J_002672"
     CDS             298..1023
                     /locus_tag="ACHU9J_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1906171.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1516..2262
                     /gene="mipA"
                     /locus_tag="ACHU9J_002673"
     CDS             1516..2262
                     /gene="mipA"
                     /locus_tag="ACHU9J_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1906172.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2352..3206
                     /gene="yeaE"
                     /locus_tag="ACHU9J_002674"
     CDS             2352..3206
                     /gene="yeaE"
                     /locus_tag="ACHU9J_002674"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1906173.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3257..4141)
                     /locus_tag="ACHU9J_002675"
     CDS             complement(3257..4141)
                     /locus_tag="ACHU9J_002675"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1906174.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4225..5220)
                     /gene="gapA"
                     /locus_tag="ACHU9J_002676"
     CDS             complement(4225..5220)
                     /gene="gapA"
                     /locus_tag="ACHU9J_002676"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1906175.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5562..5975
                     /gene="msrB"
                     /locus_tag="ACHU9J_002677"
     CDS             5562..5975
                     /gene="msrB"
                     /locus_tag="ACHU9J_002677"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1906176.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6017..6289
                     /locus_tag="ACHU9J_002678"
     CDS             6017..6289
                     /locus_tag="ACHU9J_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1906177.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6450..7091)
                     /gene="pncA"
                     /locus_tag="ACHU9J_002679"
     CDS             complement(6450..7091)
                     /gene="pncA"
                     /locus_tag="ACHU9J_002679"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1906178.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7102..8118)
                     /gene="ansA"
                     /locus_tag="ACHU9J_002680"
     CDS             complement(7102..8118)
                     /gene="ansA"
                     /locus_tag="ACHU9J_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1906179.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8285..10141)
                     /gene="sppA"
                     /locus_tag="ACHU9J_002681"
     CDS             complement(8285..10141)
                     /gene="sppA"
                     /locus_tag="ACHU9J_002681"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1906180.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10302..10853
                     /locus_tag="ACHU9J_002682"
     CDS             10302..10853
                     /locus_tag="ACHU9J_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1906181.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10970..12013
                     /gene="selD"
                     /locus_tag="ACHU9J_002683"
     CDS             10970..12013
                     /gene="selD"
                     /locus_tag="ACHU9J_002683"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1906182.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            12018..13979
                     /gene="topB"
                     /locus_tag="ACHU9J_002684"
     CDS             12018..13979
                     /gene="topB"
                     /locus_tag="ACHU9J_002684"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1906183.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3595 a         3375 c         3640 g         3396 t
ORIGIN      
        1 agttgggggt ctatatataa tccatacata gaaacagcgg agatagcaat accgaaaacc
       61 atccacatat ctatagggct tttatttgtc gatttgaccg tagtgtactt tttgactaag
      121 tccgtacctg atagcactgt taaaattaat gataatatag cgagggcagt atttacatta
      181 aaatttagct tagcgaataa aagcaacatt agtaaaataa aaataagtaa cgctatgatt
      241 aaaaatattc ctttcttgtc cataaatacc ccgtcgtatt tttaaaaaag gtatcttatg
      301 gcaaggataa gtaaaaagga atctgctaat cacaataaag tgatggaatt aatacattca
      361 gataagctcc taacacaaga agatagggaa ttcatattca ccaattatag gggggatggg
      421 ataggggcta caggtgcctt cttcacacct gaaattttag catgggattt tgtacttgat
      481 gctgactgta ctggtgactg tattgaacta tgtgctggta tcggtcgcct cagctactac
      541 cagtacttaa ggaataaacc aacgcatatt acatgcgtgg aactcaatcc tgagtacgta
      601 atgatcggga agagggtact accagaggca gaatggatca ctggtaacgc actacagtac
      661 tcaccagacc gtttctatga tgttgcctat ggtaatccac cctttgggaa gatcagtacc
      721 tcaaaagcgt atacaggccg ttataagggt agtgaatttg aatacaaggt gattgaacat
      781 gcaagtacct ttgcgtccta tggtgcatgg atagtaccgc aaggttccgc aggctttgtc
      841 tacagtgggg tacattgtta tgagcgtaga gaatcatgta agtaccagaa gtttgtgaag
      901 gatactggct atgagctaca tccaggtgta ggcatcgata caagtatcta cagggatgaa
      961 tggaatggta ctaaggtgat atgcgaatcg gtgttagtag atgatgacgg tacaaatctg
     1021 taactaaatt agttaagaaa tcataaagat agtagtactt gcaaatgttc ccattgatga
     1081 agttatgcgt cctttcgttt tttagcacag gttaagagcc gttcatatga atagtagagg
     1141 ctcccggaag aggtaaactg aacgacgtct cttgctaaaa cacagcaact ctcatgccat
     1201 tttatcgacg atgagaacaa cgtgcccggt gatacacctt caaaattaag cgtagatggc
     1261 aaatggaaaa cttgcctgca cggctttctt aatttcaatg gcatatcaac aactcatcca
     1321 taaattttgc ataattaatg taaagacaag gctcgccagt aacgctaaat tcatttggct
     1381 gtaagcgcgg tgtcatccgc gtcaggaaaa ttaaacagtt actttaaaaa atgaaaacgt
     1441 aaaaaggttg ggtttcgatg tattgacggg taaactttgt cgcccgctaa acatttgttt
     1501 aaggaatgat taattgtgac caaactcaaa cttctggcac ttggagtgct tatcgcaacg
     1561 tctgcaggcg tagcgcacgc tgaaggtaaa ttttccctgg gcgcaggcgt cggtgtcgtt
     1621 gaacacccat ataaagatta cgataccgat gtttacccag taccggtaat caactatgaa
     1681 ggcgataact tctggttccg tggcttaggt ggtggttact acctgtggaa tgacgcaacg
     1741 gacaaacttt caattaccgc ttattggtcg ccgctttact tcaaagcgaa agacagtggc
     1801 gatcaccaaa tgcgtcacct ggatgaccgt aagagcacca tgatggctgg tctgtcttat
     1861 gctcacttta cccagtacgg ttacctgcgt accaccctgg ctggcgatac cctggataac
     1921 agcaacggca ttgtctggga tatggcctgg ttgtatcgtt acaccaacgg cggcctgaca
     1981 gtgaccccgg gtatcggtgt gcagtggaac agcgaaaacc agaacgaata ctattatggc
     2041 gtatcgcgca aagagtccgc tcgcagcggt ctgcgtggct ataacccgaa cgacagctgg
     2101 agcccttacc tggagctgag cgccagctac aacttcctcg gcgactggag tgtttacggt
     2161 accgcgcgct atacccgtct gtctgatgaa gttactgaca gcccgatggt ggataaatcc
     2221 tggactggcc tgatttctac cgggatcacc tacaaattct gataatgcat ctttccaggg
     2281 cattttgtgc ataaaaacag ggcgtcacgc cctgttttgc attacggtgg tgcaatacct
     2341 gggaggaaac aatgcaacaa aaaatgattc aatttagtgg cgatgtctca ctgccagcca
     2401 tagggcaggg aacatggtat atgggcgaag atgccagtca gcgcaaaaca gaagttgctg
     2461 cactacgcgc gggcattgag ctcggtttaa ccctcattga taccgccgaa atgtatgccg
     2521 atggcggtgc cgaaaaggtg gttggggaag cattaaccgg tctgcgtgat aacgtctttc
     2581 tcgtctctaa agtctatccg tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag
     2641 ccagtttacg ccgtctcaat actgattatc tcgatctcta cttattacac aggtctggca
     2701 gtttcgcttt tgaagagact gttgcagcga tggaaaaatt gatcgcccag ggaaaaatcc
     2761 gccgctgggg cgtttctaac cttgattatg ctgatatgca ggaactctgg cagctgccgg
     2821 ggggaaatca gtgtgccact aatcaggtgc tttaccatct cggttcacga gggattgagt
     2881 acgatctact cccctggtgc cagcaacagc agatgccggt gatggcttac agtccattag
     2941 cccaggccgg gcggttgcgc aatggactgt taaaaaacgc ggtagtcaac gaaattgcac
     3001 atgctcacaa tatcagctcg gcacaagtat tgttggcgtg ggtaatcagc catcagggtg
     3061 tgatggcgat tccaaaagcg gccacgattg cccatgttca acagaatgcg gctgtgcttg
     3121 aggtcgaact ttcgtcagcg gaattaacca tgctggataa agcgtatccg gcaccaaaag
     3181 gaaaaactgc gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac
     3241 acaacattaa cgcaaattaa cgtttcgcaa cgcgaatgga ttgcgccaga tgtgcaggtt
     3301 tctctttggt gactttttgc gtttctgaag cgtaagccgt ttctacacaa acaaatgttt
     3361 tgtagccatc atccggcata tcgcccatgc taattgaaag cgccggtccc gggttccagc
     3421 ctacaacgtt cagatgatgc tggtggccta cggcgataat acgattcagc gcttcatcat
     3481 taatcacgct gcaatcttgc ggattcagat acacgcgatc ggtacggtca gggaaggtct
     3541 gaataccgtc ggtcagtaca tcttctttcg cgtcattcac tttatcaatg aagcgatcgc
     3601 ccagcccact gacgcttacc ttagcgatat cacccacgtt aaagtaggta tgcagggcag
     3661 aggtggtttc aaattcgcca tgtgattcaa gatcgatttc acaggtttta cccacgcgga
     3721 aatgcgctaa cagcgtaaag tcgtgcggac agaatttttt cgtctcttcg ctttgcgtca
     3781 attcaaaagt cagcgctacg ccatcagcat cttcattatg tgatttcagc gtccacggca
     3841 ggttgcgggc aaaaccgtgc gcaggcagac cttgttgtgc cgccggacca aaccacggcc
     3901 agcaaaccgg tacgccaccg cgaatagcga cgccattttt gaacggtgtg ttgttgctca
     3961 accacagaac ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg
     4021 caaaagaggc ttttacttgg ggatgatcga ccacaatgag gtccagttca tccagtttac
     4081 gacgggagag gacaggggag atttgttcga tgaccggaag ggcaaaaatt ttcttaatca
     4141 tgacgcagtc ctttaacttc attttatcag gtaaaaaaaa gagcgaccga agtcgctctt
     4201 tttagatcac agtttcatct caacttattt ggagatgtga gcgatcaggt ccagaacttt
     4261 gttggagtaa ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag
     4321 agcgatacca gctttagcat cgaacacgga agtgcaaact tcgccgttga aatcggtaga
     4381 tactacgtca tcttcggtgt agcccagaac gcctttcatt tcgccttcag cagcggcttt
     4441 aacggcagct ttgatctgct cgtaagttgc agctttttcc agacgaacgg tcaggtcaac
     4501 tacggatacg ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc cattcagttc
     4561 tggcagtact ttacctacag ctttagcagc accggtagag gacgggatga tgttctggga
     4621 agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca tcaacggttt tctgagtagc
     4681 ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat cgttgataac
     4741 tttagccagc ggagccaggc agttggtggt gcaggaagcg ttggaaacga tgtcctggcc
     4801 agcatatttg tcgaagttag cgcctttaac gaacatcgga gtgttgtctt tagacggacc
     4861 agtcataacc actttcttcg caccagcggt gatgtgttta cgagcagttt cgtcagtcag
     4921 gaacagacca gttgcttcag cgacaacgtc aacaccaact tcgtcccatt tcaggttagc
     4981 cggatcacgt tcagcggtaa cacggatttt tttaccgtta acgatcagat gaccgtcttt
     5041 cacttcaacg gtaccgtcga aacggccgtg agtggagtca tatttcagca tgtatgccat
     5101 gtaatcagcg tctaacaggt cgttgattgc aacgatctcg atgtcagaac gtttctgagc
     5161 agcacggaaa acaatgcgac cgatacggcc aaaaccgttg atacctactt tgatagtcat
     5221 atattccacc agctatttgt tagtgaataa aaggttgcct gtaaaattac aaaaacctta
     5281 cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt
     5341 acgcgactga ctcgcctcac tcttcctttg ggcttgaaac cacatggggt cggcgccccg
     5401 aattttaaag ggcaattacg ataaaaatgt gattttcatc acgatttagg tgcagccatt
     5461 tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt tgttagaatc
     5521 agtcacgtaa tgtgagcacg attaaagtga gatgtgagca aatggctaat aaaccttcgg
     5581 cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag aatcatggga
     5641 cagagccgcc atttacgggt cgtttactgc ataacaagcg tgacggcgta tatcactgtt
     5701 tgatctgcga tgccccgctg tttcattccc aaaccaagta tgattccggc tgtggttggc
     5761 ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac ttgtcacatg
     5821 gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg catgtcttcc
     5881 ctgacgggcc gcagccaacg ggcgaacgtt attgtgttaa ctctgcttct ttacgcttca
     5941 ctgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca acttattcca
     6001 caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc ctgaggtata
     6061 ccagcgtttg tcgaccgccg ttgaactggg gaaatggccc gatggcgttg cgttgaccga
     6121 ggaacaaaaa gcgaactgcc tgcaactggt gatgctgtgg caagctcgcc ataataccga
     6181 agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca aacagcagtt
     6241 aaaagaagat tttggtatct cagcaaagcc tattgctatg tttaagtgaa tgagttatct
     6301 agtatttaag cctgttatta agggagggca aatatacttg ttaggacgtc gtgttttagc
     6361 tgcaagtagg ttaaatgcaa cactgacaag tattatctgg cctacaatgt cattgaggaa
     6421 atgtaggcca gagatgaatt caactaatat tacccctgtg tctcttccca gtctgccagc
     6481 gtatatagcg ttgccccagc tgctgacatc tccataaacg cgtgcgcact gtcctggggc
     6541 tggatattca cgccacgaca accatcggta atcacattta ccttataacc taactgtaac
     6601 gcgtccagca cggtaaactt cacgcaatag tcagtagcca ggcccataac gatcaattca
     6661 tcgatttcat gatcgcgtaa ccagtcatcg agcgaggttt tctgccgacg gccgttatca
     6721 aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt tatggaacac cgctgcgatc
     6781 gctttttggt tcagtaacgg atgtaattgt gcgccttcac tgttctgcac acagtgatct
     6841 ggccagaagg tttgtggcaa accgtcgagt tggcctggcg tataaggctc tacaccgtgc
     6901 tgactggcaa aactgccgtg attcgccggg tgccagtcct gactggcgat aaccgcttca
     6961 ccgcgcgact ggcaccagtc aatcaggcgg ttagcgacat ccaccgtact gtcaccttcc
     7021 ggcacggcga gcgcgccacc agcacagaaa tcattttgta aatcgaccag taacagggcg
     7081 cgagggggca ttacagtctc cttaatcatc cggcgtcagt tcgccgcgca ggttttggct
     7141 catggccttg cgaatggttt cagtatccag ttcctggctc agcaggtaat gcagtttggt
     7201 tagcgtggct tctacagtca tatctgcacc gccaataacg ccggcatggg cgagggcgtt
     7261 accggtggca taaccaccca tattcacttt accggacata cattgtgtca ggttgaccac
     7321 cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc
     7381 attacccacg ccataggagc gcagaatcaa tgctttcacc ggttggcgca gaaaattgcg
     7441 cacgacgtca gcagaaatcc ctggataaat cgtcactacg ccaattggtt gtggcgtgat
     7501 tggatgaacg attaattccc cttcaccgtg cggggcgggt ggcgtattca aacgacgaat
     7561 atggatacca gcttccagta acggaggaag gtttggagag gcaaacgcat caaaaccatc
     7621 ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg ttattgaaaa agagcgttac
     7681 ttcgttgatc ggataattcg ccgcaacgta caacgcattc agcagattaa tttgtccgtc
     7741 agagcgtaac tcagccagcg ggatttgtga ccctgtcaca atgaccggtt taccgagatt
     7801 ctcgagcatg aacgacagcg cagaggcagt atacgccatc gtgtcggtgc cgtgcaggat
     7861 gacaaaacca tcatagtcgt catagtgcgc tttaatatct tcagcaatat gctgccagtc
     7921 ttctggcgtc atatcagaag aatccatcag cggcgtatat tcatgaatgg tgaaatctgg
     7981 catctccggg cgatggaatt ccggcatcag cgccagttgg cgttgtagat gacctgacac
     8041 cggaatataa ccctgctcgg aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta
     8101 aattgatttc ttttgcatga tattgatatg aatgatatcg aaagagcaaa agtatatacg
     8161 tgaggcagga ggagcgcacc tgatactatg cggataagga aatatggggc gagacagcgg
     8221 gaagaggatt cgtcgacgta aaaaataatg tggccgacga tctgtcggcc actcagtaca
     8281 agacttaacg cacgttggcg caggtcaggc aaaacgcata acggttttgt gggtcgttaa
     8341 acgcggccag cttgtcgctt tcacttttaa cggtagaggc taccgaggca agcggtgcag
     8401 gtaacatggc ctggaacgca tctggcaaca ttgcccggac agaaccagac atgttgtcca
     8461 tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc cagatgccac tgtttcactt
     8521 ttgccagctc tgctgctttg gcgaccgcat catcgaaatc cccgagacta tcgaccagcc
     8581 cgttagcttt tgcatcctga ccggtccaga cgtggccctg ggcgatttta tcaatctgct
     8641 ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa gcgtttatag ccattctcaa
     8701 tgcttaattg catcatctgc tgcgcttccg gcggcagtgc cctggtgata gaaacatccg
     8761 ccagcggtga agttgagaca ccatcagtat gaacaccaat cgaatccaga ctattttcta
     8821 cggtggtgat cacgccgaag ataccgatag aaccggtcag ggtgctgggg ttagccacaa
     8881 tgtaattagc tggcgtggaa atccagtaac caccagatgc cgccatgccg cccatcgata
     8941 caaccacagg cttacccgct gcccgggctg ctgccagttc agcgcgaatc acttcagacg
     9001 cggtaacgct gccgcctggg ctattaacac gcaggacaat cgctttcact ttcgggtcaa
     9061 ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc aacattcccc tgagtttcct
     9121 cgccatccat aattgcgcca ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg
     9181 gcgttttcaa tgcgtaatcg taataactga tggcgcgata atttttatca gtcttactcc
     9241 agccgaactc tttggttaga gctttttcga tttcggcact cgatgccagt gcatcgacca
     9301 gcttgttttc cagtgcatat ttcgcggtat cgccaccggt tttggttaaa ccctcaagca
     9361 acccttgcgc gccagggaat acctgctgag cagggatctg ccggttagcg gcaacagtat
     9421 tcagatagtt ttgccacagc tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg
     9481 acatgtcatc acgaataaac ggttcaacgg cagatttata cgtacccacg cggaacacat
     9541 gggtggaaac tttcagctta tccagcaacg atttgtagta cagaccgttg gtagcaaaac
     9601 cgtgcaaatc caccacgcct tgcggtgaca gccaaatttt attggcgaaa ctggcgagat
     9661 aatattgccc ctggctgtag ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa
     9721 actctttcag ggctttgccg atgtactgca tagacggctg gtcgccgcct gcgaagtttt
     9781 tcagatccat cacgataccg gtgatattgc ggtcgtcctt cgcctggcga atggtgttga
     9841 cgatatcaaa cagtgagttt tcctgcagac gatcggaact ggcaccaagc agctggcggc
     9901 ttaatttact aaaccgctga gaactgtcgg gtttatcgac gatcacacca gaaatgtcca
     9961 gcagcagtgc gccacgactg gccgtttctt tcgaatcacc accactgacc tgcatccaga
    10021 tccccacacc aaccagtacg aggaaaataa agaacaggtt aagtaccatt tcacggacga
    10081 aattcagcag acgccacgtc catttaaaaa atccggcaat aaatcgccaa agggttcgca
    10141 tgtattctcc caacttaagg tcacacctgt cacaggcgca atagatgtgg ttatcgtaat
    10201 gacccaaccg gcacttgtca gcaggaattg cggactgtgc tgtaacaaaa tccactctcg
    10261 tgttaatttt gtgaataaat atcacgacag gagttaatca aatggatgca ctcgaactat
    10321 tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc gccaacgggt gaacaactgc
    10381 aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt
    10441 tttttgtgat tgaaggggaa gggcgcgagc gtttcagcgc cgtactggaa cagggggcga
    10501 ttgctgccgg tagtgatgac aaagctatcg acaaagcccg taatgcgccg ttccgcgcac
    10561 cgctcatcat cacggtggtg gcgaaatgcg aagaaaatca taaagtcccg cgatgggaac
    10621 aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat
    10681 ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg
    10741 gttgccgtga gcaggataaa attgtcggtt ttctctacct cggcacgccg cagttgaaag
    10801 catctacgtc gattaacgtc ccggacccga cgccgtttgt aacttatttc tgataattgt
    10861 cgcgaaactg tctggattgt gagcaaaggc gcaggaattc agacactctc acttatcact
    10921 tcacggaatg agggttacca tacccggatt gcattgacag gagatgtcca tgagcgagaa
    10981 ctcgattcgt ttgacccaat acagccacgg agctggttgc ggctgtaaaa tttccccaaa
    11041 agtgttggaa accatcctgc acagtgagca ggcgaagttt gttgatccga atttgcttgt
    11101 gggtaatgag acccgcgacg atgcggcggt gtacgatctg ggcaatggca ccagcgttat
    11161 cagtaccacc gacttcttta tgccgatcgt tgataatcct ttcgattttg gccgcattgc
    11221 ggcgactaac gccatcagcg atatcttcgc gatggggggc aaaccgatta tggcgattgc
    11281 gatcctcggc tggccgatta acaaactttc cccggaaatt gcccgcgaag taaccgaagg
    11341 tggacgctat gcatgccgtc aggcgggtat tgcgctggct ggcggtcact ccatcgatgc
    11401 gccggagccg atttttggtc tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa
    11461 aaacagtacc gcacaagccg gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt
    11521 tcttaccacg gctgagaaaa aatcactgct gaaaccagaa catcagggac tagcgacgga
    11581 agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc
    11641 gatgaccgac gttacgggtt ttggtctgct gggccatttg agcgaaatgt gtcagggcgc
    11701 aggtgtgcag gcgcgcgttg actatgacgc gatcccaaaa ttgccggggg ttgaagagta
    11761 cattaagttg ggcgcagtgc ctggcggcac cgaacgtaac tttgccagct acggtcatct
    11821 gatgggtgaa atgccgcgtg aagtgcgcga tttgctgtgc gatccgcaaa cttctggcgg
    11881 tttgctgctg gcggtcatgc cggaagcaga aaatgaggtc aaagctacag ccgccgagtt
    11941 tggcattgaa ctgacggcaa ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt
    12001 tgagattcgt taattcaatg cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca
    12061 ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg
    12121 gtcaggtggt gacctggtgt atcggtcacc tgcttgagca ggcgcagcca gacgcctacg
    12181 acagccgcta tgcgcgctgg aatcttgcgg atttgccgat tgtcccggaa aagtggcaat
    12241 tacagccccg accctccgtg accaaacaac ttaacgtcat caaacgcttc ctgcatgaag
    12301 ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg gcaactgctg gtggatgaag
    12361 tgctggacta tctgcaactg gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa
    12421 acgacctgaa cccgcaggcg gttgagcggg cgctcgaccg tcttcggtcc aacagtgagt
    12481 ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc cgactggctg tacggcatca
    12541 atatgacccg tgcgtatacc attctcggtc gcaatgccgg ttatcagggc gtactttccg
    12601 tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa
    12661 acttcgtggc gaaagacttc tttgaagtca aagcacatat cgtgacacct gccgatgagc
    12721 ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc
    12781 gcttgttaca tcgtccactg gcggagcatg tggttaaccg cattagtggt caaccagcaa
    12841 ttgtcaccag ctataacgat aaacgggaat cagaatccgc gccgctgcct ttttcgcttt
    12901 cggcgttgca gattgaagcg gcaaaacgct ttggtctgag cgcgcagaac gtgcttgata
    12961 tctgccagaa gctgtacgaa acgcacaagc taatcactta tccgcgttcc gattgtcgct
    13021 atttgccaga agaacatttt gccggacgcc acgcggtgat gaatgccatc agcgttcatg
    13081 caccagatct gttgctgcag ccagtggtag atccagatat acgcaaccgc tgttgggatg
    13141 acaaaaaggt cgatgcacac cacgccatca tcccgaccgc acggagttca gccatcaacc
    13201 tgacggagaa cgaagcgaag gtctataacc tgattgcccg tcagtacttg atgcagttct
    13261 gcccggatgt ggtgttccgc aagtgtgtta tcgaactgga cattgccaaa ggcaaatttg
    13321 tcgctaaagc gcgttttctt gctgaagcag gctggcgcac gctgttaggc agcaaagagc
    13381 gcgatgaaga aaacgacggt acgccattgc ctgtggtggc gaaaggcgat gagttgctgt
    13441 gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc gcgccatttt accgatgcaa
    13501 cactgctttc ggcgatgacc gggatcgcgc gctttgtgca ggacaaagat ctgaaaaaga
    13561 tccttcgtgc gaccgatggt ctggggacag aagcaacccg tgccgggatt attgaactgc
    13621 tgttcaagcg tggttttctg accaaaaaag ggcgctatat ccactctacc gacgccggaa
    13681 aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg
    13741 aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc
    13801 tggtggggac gctatatcag cttattgatc aagccaaacg tacgccggtg cggcagtttc
    13861 gcggcattgt ggctccgggc agtggtggca gtgctgataa gaaaaaggct gcaccgcgta
    13921 aacgtagtgc gaaaaaaagt ccgccagcag atgaagtcgg aagcggggcg atagcgtaag
    13981 cgagtgaatc tttcgtgcta ttcgag
//
LOCUS       DAZPJP010000102        13886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPJP010000102 DAZPJP010000000
VERSION     DAZPJP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.102"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHU9J_002685"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHU9J_002685"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1906184.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHU9J_002686"
     CDS             2273..2662
                     /locus_tag="ACHU9J_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1906185.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHU9J_002687"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHU9J_002687"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1906186.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHU9J_002688"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHU9J_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1906187.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHU9J_002689"
     CDS             complement(4321..4521)
                     /locus_tag="ACHU9J_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1906188.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHU9J_002690"
     CDS             4687..5232
                     /locus_tag="ACHU9J_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1906189.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHU9J_002691"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHU9J_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHU9J_002692"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHU9J_002692"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1906190.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHU9J_002693"
     CDS             complement(6529..7353)
                     /locus_tag="ACHU9J_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1906191.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQILSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHU9J_002694"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHU9J_002694"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1906192.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHU9J_002695"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHU9J_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1906193.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHU9J_002696"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHU9J_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1906194.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHU9J_002697"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHU9J_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1906195.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHU9J_002698"
     CDS             complement(11316..11969)
                     /locus_tag="ACHU9J_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1906196.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHU9J_002699"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHU9J_002699"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1906197.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHU9J_002700"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHU9J_002700"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1906198.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3361 a         3519 c         3694 g         3312 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaaggatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagag
//
LOCUS       DAZPJP010000103        13822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPJP010000103 DAZPJP010000000
VERSION     DAZPJP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.103"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1124
                     /locus_tag="ACHU9J_002701"
     CDS             <1..1124
                     /locus_tag="ACHU9J_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1906199.1"
                     /translation="KFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQYDLDKMV
                     NIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPELTTPAG
                     QYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQVDVSVA
                     AFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQNGVKTKV
                     NLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGMTLAEAL
                     GNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIV
                     YVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            1112..1558
                     /gene="etp"
                     /locus_tag="ACHU9J_002702"
                     /gene_synonym="yccY"
     CDS             1112..1558
                     /gene="etp"
                     /locus_tag="ACHU9J_002702"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1906200.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            1578..3758
                     /gene="etk"
                     /locus_tag="ACHU9J_002703"
     CDS             1578..3758
                     /gene="etk"
                     /locus_tag="ACHU9J_002703"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1906201.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(3873..5171)
                     /gene="appA"
                     /locus_tag="ACHU9J_002704"
     CDS             complement(3873..5171)
                     /gene="appA"
                     /locus_tag="ACHU9J_002704"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1906202.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(5251..5343)
                     /gene="cbdX"
                     /locus_tag="ACHU9J_002705"
     CDS             complement(5251..5343)
                     /gene="cbdX"
                     /locus_tag="ACHU9J_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1906203.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(5356..6492)
                     /gene="appB"
                     /locus_tag="ACHU9J_002706"
     CDS             complement(5356..6492)
                     /gene="appB"
                     /locus_tag="ACHU9J_002706"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1906204.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(6504..8048)
                     /gene="appC"
                     /locus_tag="ACHU9J_002707"
     CDS             complement(6504..8048)
                     /gene="appC"
                     /locus_tag="ACHU9J_002707"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1906205.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(8182..9039)
                     /locus_tag="ACHU9J_002708"
     CDS             complement(8182..9039)
                     /locus_tag="ACHU9J_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1906206.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(9096..9434)
                     /gene="hyaE"
                     /locus_tag="ACHU9J_002709"
     CDS             complement(9096..9434)
                     /gene="hyaE"
                     /locus_tag="ACHU9J_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1906207.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(9431..10018)
                     /gene="hyaD"
                     /locus_tag="ACHU9J_002710"
     CDS             complement(9431..10018)
                     /gene="hyaD"
                     /locus_tag="ACHU9J_002710"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1906208.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(10015..10721)
                     /gene="hyaC"
                     /locus_tag="ACHU9J_002711"
                     /pseudo
     CDS             complement(10015..10721)
                     /gene="hyaC"
                     /locus_tag="ACHU9J_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(10740..12533)
                     /gene="hyaB"
                     /locus_tag="ACHU9J_002712"
     CDS             complement(10740..12533)
                     /gene="hyaB"
                     /locus_tag="ACHU9J_002712"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1906209.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(12530..13648)
                     /gene="hyaA"
                     /locus_tag="ACHU9J_002713"
     CDS             complement(12530..13648)
                     /gene="hyaA"
                     /locus_tag="ACHU9J_002713"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1906210.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         3352 a         3767 c         3629 g         3074 t
ORIGIN      
        1 ttaagttttc ggtactgaca ttggctgttc tgtctttgac agcatgtacc cttgttcctg
       61 gtcagaattt atcgaccagt aacaaggatg tcattgaact acctgacaat cagtatgacc
      121 tggacaaaat ggtcaatatc tatcccgtca cgccaggatt gattgatcag ttgcgcgcca
      181 aaccgattat gtcgcaggct aacccggagc tggagcagca aatcgcaaac tatgaatatc
      241 gtatcgggat tggtgatgtg ttgatggtta cggtctggga tcacccggaa ctaaccaccc
      301 ccgcagggca ataccgtagc gccagtgata ccggcaactg ggttaacgcc gatggtgcga
      361 ttttctaccc ctatattggt cgcctgaaag tggccgggaa aacacttacc caggtacgca
      421 acgagattac agcgcgtctc gattcagtca ttgaaagccc gcaagtcgat gtcagcgtcg
      481 cggcgttccg atcgcaaaaa gcgtacgtca ccggcgaagt cagcaaatcg ggtcagcagc
      541 cgattaccaa tattcctctg accatcatgg atgcgattaa cgccgcgggc ggtctgaccg
      601 cagatgcaga ctggcgcaac gtggtgctca cccagaatgg cgtaaagacc aaagtgaacc
      661 tctatgcgct gatgcagcgt ggcgacttgc gccagaacaa actgctgcac ccgggcgata
      721 ttttatttat tccgcgcaac gatgatctga aagtcttcgt gatgggtgaa gtgggtaaac
      781 agagcacgct gaaaatggac cgcagcggta tgaccctggc ggaagcgctg ggtaacgctg
      841 agggtatgaa tcaggatgtc gctgatgcga cggggatttt tgttattcgt gcaacgcaaa
      901 acaagcagaa tggcaaaatc gccaatattt atcagctgaa tgcgaaggac gcatcggcaa
      961 tgatactcgg aacggaattc cagcttgaac cttacgacat tgtttatgtc actaccgcgc
     1021 cgctggctcg ttggaaccgt gtgatttctc tgctggtacc gacgatttcc ggggtgcatg
     1081 acctgacaga gacttcacgg tggattcaaa catggcccaa ctaaaattta actcaatcct
     1141 ggtggtttgt accggcaaca tttgccgttc gcccattggc gagcgcttgc tgcgtaaacg
     1201 tctgccgggc gtaaaagtca aatcggcagg tgttcatggt ctgataaaac accctgcaga
     1261 tgcgacagcg gcagatgttg ccgctaatca tggcgtatct cttgaaggac atgccggacg
     1321 taagctcact gctgagatgg cacgaaatta cgatctgatt ctggcaatgg agtcggaaca
     1381 tattgctcag gttaccgcaa ttgctcccga ggtccgcggg aagacaatgc tgtttgggca
     1441 atggctggaa cagaaagaga tcccggatcc ctatcgtaaa agtcaggacg catttgaaca
     1501 tgtctacggt atgttggagc gcgccagtca ggaatgggcg aagcgcctca gccgataatt
     1561 tgagtttata aaatacgatg acaactaaaa atatgaatac gccaccaggc agcactcagg
     1621 aaaatgagat cgatctgctt cgtctggtcg gcgagttatg ggatcaccgt aagtttatta
     1681 tcagcgtgac cgcgttattc acgctgatcg ctgtcgctta ctcgctgtta agcacaccaa
     1741 tttatcaggc agatactctg gtccaggttg agcaaaaaca gggcaacgcc attctcagcg
     1801 gcctgagcga tatgatccct aactcatcgc ccgagtctgc accggagatc caactgctgc
     1861 aatcgcgcat gattctcggt aaaaccattg ctgaactgaa tctgcgcgac atagttgagc
     1921 agaagtattt tccgattgtg ggtcgcggct gggcgagatt aaccaaagaa aaaccaggtg
     1981 agctggcgat cagctggatg catattccac aactgaatgg tcaggatcag caactgacac
     2041 tcacggttgg ggaaaacggc cactatacac tggaaggtga agagttcacc gtcaatggta
     2101 tggtcggcca gcgtctggaa aaagatggcg ttgcgctgac tatcgcggac attaaggcca
     2161 aaccaggaac acagtttgtc ctgagccagc gtaccgaact ggaagcgatt aacgcattgc
     2221 aggaaacctt taccgttagc gaacgcagta aagaaagcgg gatgctggaa cttaccatga
     2281 ctggtgatga tccccagttg attactcgta ttctgaacag catcgctaac aactatttgc
     2341 aacagaatat cgctcgccag gcggcgcagg attcacaaag ccttgaattc ttacagcgcc
     2401 agttacctga agtgcgcagc gagctggacc aggcggaaga aaaactcaac gtttatcgcc
     2461 agcagcgcga ttcggttgac cttaacctgg aagccaaagc cgttcttgag cagattgtga
     2521 acgttgataa tcaactcaat gagctgacct tccgcgaggc agagatctcc cagctgtata
     2581 agaaagatca cccaacttat cgtgcgctgc tggaaaaacg ccagacgctg gagcaagaac
     2641 gcaaacgcct gaataagcgg gtatcggcaa tgccttccac ccaacaggaa gtgttgcgtt
     2701 taagtcgtga cgtagaagcg ggccgtgcgg tatatctgca attacttaac cgccagcagg
     2761 agttgagtat ttcgaaatcc agtgccattg gtaacgtgcg gattatcgac ccggcagtca
     2821 ctcagccgca gccagtgaaa ccgaaaaaag cgttgaatgt ggtgcttggt tttattcttg
     2881 gcctgtttat ttctgtgggt gccgtgctgg cgcgtgcgat gttgcgtcgt ggtgtagaag
     2941 ccccggaaca actggaagag cacggcatca gcgtttatgc cactatccca atgtccgagt
     3001 ggctggataa acgcacccgt ctgcgtaaga aaaatttatt ttctaatcag cagcgccatc
     3061 gtactaaaaa tatccccttc ctggcggtgg ataacccggc ggattctgct gtggaagccg
     3121 tacgtgcgct acgaaccagt ctgcatttcg ctatgatgga gacggagaat aacattctga
     3181 tgatcaccgg tgcgacgcca gacagtggta aaacgtttgt cagttcaact ctggcagcgg
     3241 tgatcgccca gtccgatcaa aaagtgttat ttattgatgc cgacttacgc cgtggttatt
     3301 cgcataacct gtttaccgtg agtaatgaac atggcttgtc ggaatatctg gcaggtaaag
     3361 atgagctcaa caaagtgatc cagcattttg gcaaaggagg ctttgatgtg attactcgcg
     3421 gtcaggtgcc acctaacccg tctgaactgc tgatgcgcga tcggatgcgt caattactgg
     3481 aatgggcgaa cgaccattac gatctggtga ttgtcgatac gccgccgatg ctggcggtga
     3541 gtgatgccgc ggtcgtgggg cgttctgttg gcaccagcct gctggttgcg cgttttggct
     3601 tgaacaccgc caaagaggtg agtttgtcaa tgcagcgtct ggaacaggca ggcgtcaata
     3661 ttaaaggcgc tatcctcaat ggtgtgatta aacgcgccag caccgcttac agttacggct
     3721 ataactatta cggttatagt tactccgaga aagagtaaaa ctgaaagtgg ccggatgcgg
     3781 cgtgaacgcc ttttccggtc tacgcattag acagttcttc gtttgtcatc agcctcagag
     3841 cattcaggta actgaatgct cttttttatg cattacaaac tgcacgccgg tatgcgtgct
     3901 tcattcacga tttgcgtaaa acctgccaac gaacacatgc cctgcgcatt tcgctcttca
     3961 catcctgcca gggtcagttt cacctctccg ggcggcgtat ttaatgacag cggcgtttta
     4021 tcacgcatct gctgtaaagt ctggaagacc agcgaaacct gaatccactg gctgttatcg
     4081 cttagccgac gccagcgttc aaacaccagt tcaccacctg gcggcgtgtt atccggctga
     4141 ccgggaagcg tccagttgag ctccagtgcg ccgccgagat ttgccagatt agtatcgtgt
     4201 ccggcgataa acagcactga agtgggtaat gtcacaccat acgcctgttt ttgcggtgga
     4261 tggggcgtca acgctgtctt gatcaaatct aataacgggg tggcgcggct gcgggcaacc
     4321 tctggcgtgc gttgtagcaa ataaaattgc gcgttatgca aacttagcaa ggtgttccac
     4381 tggtgtgaat cggtgatcct tccccacccc ggctccggca ttccctgtgc ttgttgcagg
     4441 agaaatatct ccgtcagcat tgatgcgagg cttaccgcac cggttaatga gacattgtcg
     4501 gcgctcacct tgagttccga tggtaatgcc tgcgttaatg aacagctttc gtcctgtttc
     4561 tcacgtttaa ggcacaagtt tgattgcgga aaattaagca cccgttccag ttcgcgaaac
     4621 gccgtttgcc gatgcccggt aaagtcagca attgaccctc ctgccctgct gaggatcgcg
     4681 tcagtcacgt tcgcgttatc cagttggcaa acgccagttt ttagaggatt aaataacgga
     4741 tcgggactgg acgtatcagc ctgggtatgt acggttattg cacagtcagg tgccagcccg
     4801 gcggcgaagg cttcgcctgt tttacgggta cgctcgtcga catcagcaat aatcgcgacc
     4861 tgaccagact gcgggcagcc ctttttcgcc agcaatccgt cggctaccag acgctggcgt
     4921 tggtaatgtc cgagataggc gattagctca ccaccgcgcg gtgtcagcca acccagtttt
     4981 accggccagg ttggccatgc gtctggggtg acatcctgca tcagttgcgt ggccttggtt
     5041 ggagcacgca caccatgacg actgacaatc accacacttt ccagcttcag ctccggctca
     5101 ctctgagcga atgcagattg cggggttaac ggaatcagaa gagataaaaa tgggattaag
     5161 atcgctttca tcgatatgtt tccgcttttc atatctgaca ttattgattg cctgatgcga
     5221 tgctaatgcg ccgcatcagg cctacgttcg ttaacttttc agacgcgggt ccagccatac
     5281 caacacaagg gtggagagcg aacacatcaa caaaatgccg acgaaccaaa gtaaatacca
     5341 cattgttcct gctccttagt acaactcgtt ttcgttacgg cggagagttt ctgttgtcat
     5401 gcgtccccac attttgtagt agctccagag agtgtagagc aacacaatgg gcaaaaatat
     5461 cagcacgatt accaacataa tgctcagcgt cagctgactg gaagtgctgt cccacaacgt
     5521 caggctggag attggactca cgcttgacgg catgacaaag gggaacagcg tgatgcctgc
     5581 cgtgaaaatc acgccaaatt gcatcaatga cgccattaaa aatccccaac ccgggcgacc
     5641 acgataaatc gccatcaccg tcagcaatgg gcagaagaac cccagcagcg ggaagatcca
     5701 caaaacgggc gattcgacga aattattcat ccaggcacca ggtagcactg ccaccagttt
     5761 cattaacgga ttggaaggac cgttagcatc ctgggcgagc agtacaaagc catcaatacc
     5821 gacccacagc cagtaacccg ccagcaaaaa gcacagcatc accaacagtg cagcgcgttt
     5881 ggtggccagc tgtgaacgca gatgaatcac accaacagtt ttcagttgta accagacgcc
     5941 accttgcaaa atcaccatcc caaggctgag caatccgcac aataaaggga atggcgtcag
     6001 cagttgccag aagctaccga gatactccac gcgtaattgc ggtgtgaagg caaacggcac
     6061 gccgagcaac aagttgccga aggcgatacc gaagactacc ggcggcacca gactgccgat
     6121 gaccagaccg gcgtcccaca ttttacgcca gcgtgcatcg gcgatttttc cgcgataatc
     6181 aaaggccagc gggcggaaga acagtgagca cagcaccagg atcatcgcca cataaaagcc
     6241 ggaaaacgcc gctgcataca ctctgggcca ggcggcaaat aatgccccac cagcgaggat
     6301 caaccagacc tggttgcctt cccagtgtgc accaacgctg tttatcacta tccggcgttc
     6361 atcatcatta cgcgccacca gcggcagcag acagccgatc cccatgtcaa atccgtcgga
     6421 gatcataaag accaccagga tcacgccaat cagcagccac cagatgaagc gcaatgtttc
     6481 ataatcaaac atgattttcc cctttacccc tgttgctgcg tcggttgttc actctgcatc
     6541 gcgctcggcc ccagacgggc atatttctgc atcaggtaga cttcggcgat taagaacagg
     6601 gtgtaaagcc ctacgatcat gatcagtgag aaagccagtt gtcctgtggt taaagcggag
     6661 tgcgcggagt atgtcggtaa gatgtcctgt atcgcccacg gctgacgacc aaactcggtc
     6721 ataaaccacc cggcttcaat cgcaatccac ggcaacggca aactccagag cgccattttc
     6781 agcacccagc gatgctggtc gattttgcca cgcagcgtct ggacaagcgc aatcagcatc
     6841 accagtagcg gcagggaacc acagcccacc atgatgcgga aactccagaa taccggcgca
     6901 acctgaggta tcgcgccacg catcgccgcc tggtactgtg cggctgtgac atgattcata
     6961 tccggcgcat agcgggagag caacatgccg tagcccaggt caccttccag ttcccggaat
     7021 gcctgcaaca catgcggctc acgattgcct tgcgatattt cctgcattaa cagccaggcc
     7081 atacgtccgc gttgcaagcg tgggtaggtt tcagccatca aatttttcag acccggcacg
     7141 gttttatcta atgagtgagt ggcgaggatc cctaacagcg cgggaatttt gatggcaaag
     7201 gcgttacgct cttgatcctg ttccggccag gcaaccacat ggaacggtgc aggagcaggt
     7261 tccgtttgcc actccccttc catcgccgcc agttttaccg gttgtacttg cgcgacttca
     7321 tacgcagaac tgtctccgag ttgtagcgtg ccgataatcg ccagagtacc gaagacggaa
     7381 ccgatggcaa acgagcgtaa tgcaacatcg cgctcccgtc cgcgcagtaa ataccaggcg
     7441 ctgatcgcca taataaacat ggccccggtc acgtagcccg ccattacggt gtgcacaaat
     7501 ttcacctggc tgaccgggtt aaagaccagc tcgctgaagc tggtcatctc catacgtagg
     7561 gtgtcgatat caaaatgcgc accggtcggg tattgcatcc aaccgttggc attcaatatc
     7621 cacaatgcag agagatttga accgaacgcc accagccacg tcaccagcag gtgctggtat
     7681 ttattcagcc gctgccagcc gaagaagaaa agcccgacaa aggtggattc gaggaagaag
     7741 gccattaatg cttccatcgc cagcggtgcg ccaaaaatat cgcccacata gttggaatag
     7801 aatgaccagt tagtaccaaa ctgaaactcc atggtcaggc cggtagccac gccaagagca
     7861 aagttgatac cgaagagctt accccagaag cgcgtcatat cgcggtagat tgttttgccg
     7921 gtgaccacgt aaatggtttc catgatagcc agcaaaaaaa tcagccccag ggtaaggggt
     7981 acaaataaaa agtgatacag cgcggtcaga gcaaactgcc agcgcgataa atcaatgaca
     8041 tcccacatgc gcactcctgt aggcgaagag agaaaaataa cccgataatg ttagatctcc
     8101 ctctttatcc tgaaactcta cgaccagagg tattcgccga ttgcgatatt cataagactg
     8161 aaagcgatac ttaccgtctt tttacgtcgg tgcagcttcc gccagccact gacatacctc
     8221 gctaagccgc tgcgcagagt cgaccaaatc ttctggcgct gccagcacca cttccggtat
     8281 tgggcagatt tcataacttt ccagtaaagg accttttaag gtgtccgtac agcgtaaatg
     8341 ccagacatgg cgtaaccccg tggcgttgat atagctctcg ccatagccaa tggtacgaat
     8401 ctgaatattt cccggcccac agagacgtga gagaaaaagc cgatcagcct cgctgatggg
     8461 taattgcgtc agattaatgc tgtgcggctg cgcgtcgggg ttacgtacat gcgccagtaa
     8521 ctcatgcgcc aacggtaggc cattcatcag gccatcaatg ggcggcggta acagcgaatc
     8581 tgtcggcaag agattttgcg tggccgcctg ccacaacgcc agcggcgcgc agccggcctc
     8641 cagtttgtcc tccagcaact tttcgccgcg acgtttgcgc acccgccata atccgcagaa
     8701 gatcgcctcc tgtatttcac tttcgctgtc gtcagcctgc tgaatgcgca ctgacacttc
     8761 cccttccccg agaagcgtat tgataaagtg acgatcgtcg gcgttgagat ttaacagatc
     8821 gactgccagc acctcgccca acggcggctg gcgttgactt agttgatgtt gtaactcgtt
     8881 taacagcgca atcacctgcg ggctgtgagc acattgctcc agggccgcca tactcggttc
     8941 atcattcacc tgacagctga tcggcagtgg attcatgctg aaactgtcat cgttcggttg
     9001 cgttcctggc cccagcagat ggaaaaaagt ttcgctcatg aggcacgctc ctgttgtggt
     9061 tcgacaagcc cacgcatcag gtttatcagt tccgctcacg ggtgaatacc attcagcaca
     9121 ccgcgatagt ttccgccggt aaacactaat gtggcgggaa agcgaaagac gccaaagcga
     9181 tctccgatgg cttcgctctg ttcaaggtca gcaatcgcca cctgccatgt gtagccggga
     9241 aactcgcgca gtaattcgcc aatcattacc ggattatcgc tgacctctgg cgtgcgtttc
     9301 gggtcactgc ttaataacac cacgccgtct ggcgcttgcg taagccagtc gtcaagacgg
     9361 aattcactaa ccggcgtcca gccgcgcgcc agcattcgtt gccacaacgc atcaaatggc
     9421 gtgtcgttgc tcatccctgc tcctccagtg tcatcctgta ctggcgcaag cgaacgcctt
     9481 cgtaattttc catcgacaga cagtcataat tgagacagcg cgattcatta gccggttgcg
     9541 gcacaattcc ccatgcagcc agctgcgcca gcgccgcctg ttccgctgcg ggcagttgct
     9601 cccgtgccag ttcgctcagg cggccgccgt agtcgtcgag cattgcgggt tgcagaccga
     9661 cgagggcaat atgtgctggc agatgtccgc ggatatctgc cagcgccagc acttcggaga
     9721 aactgttctg gtgcaggctc attttcttcg cgctgagata agccggaata cgttctccgg
     9781 cataggttcg cagcgttcca ggttccagcc cgtagtcaat ggcatcgaga atcaacagat
     9841 ggctggcgct ttcgacatac cccagcaagt tcagtccctg agtaccgcca tcgacaatct
     9901 ccacatactc gggccagtgg taatgggcat acaaccgttc cgccacccgc acgccgaaac
     9961 cttcatcggc ccacagcagg ttgcccagcc ccattaccac aacgcgttgc tcgctcatga
    10021 acgctccttg ttacttattt tgccaaattt gtggctacgg tagccgttga ccatggtgga
    10081 gatcaccgtg tcgtcggaca tgatgtcttc acgcagcgcc atatagacat gaccgatcac
    10141 aaacgcgcca atcagccaca tccccagccg atgccagctg tgaatgtcca ttgagttgcc
    10201 tcccgtccag tagaaaaatt ccaccacata acggaacggc gcaaaaatag cgtactggct
    10261 gtgttcgctg tacagcgcaa agccggtgat gatcataaag accgacatca ggaaatagcc
    10321 gaacatcgcc gcctgggcga tggggttatg gcctatatcg gcactcggac gttttgccag
    10381 aacagatacc agcggatttc ataccacacg ccctgccacc agcttttacg ccataccggc
    10441 acgataaaca gctcgcggga gtatcgattg ccaacaaaag cccagtagat ccgcatcagc
    10501 aagaccacgg taaaaaccat cccggcgctg aagtgaatta acctgatgta gcccatatag
    10561 aacagatacg tcgcctcgcc gctgacggaa ggtagcggct tgccgataaa gtatccggtg
    10621 accatcaaca ccgccatgca taacaccgtc agccagtgcc agatgcggac tggcgcttca
    10681 aagacatagt ggctgacaac gttgtcgctt ttctgttgca tgatgattct ccttcgctgt
    10741 taacgcacct gcacggagat cagctcgcta ccgtcgtcgc ccagcacgtg tgttgaacag
    10801 gcgaggcacg ggtcaaagct gtgcagagta cgcaggatct ccagcggttg ctcggggatc
    10861 gccattttgg tgttcatcag cgccgcttca taagcgccaa tctgcccttt gggatcgcgc
    10921 gggctggcgt tccaggtggt cggcaccacg cactggtaga gatcaatctt gccatcgcga
    10981 atggcggccc agtggcctaa cgccccgcgc ggcgcttcgg taaagccgac gccacggcac
    11041 tctgtcggcc aggttgcagg ttcccatttt tccgtggagg cggtggcaag attgccgttt
    11101 ttcaggttag tcatcagctt gttgaagaaa tattgcaact tacctgcggc ccactgtgct
    11161 tcgtgcgcgc ggcacaaaat gcggcccaac gttgactgga taccggaaag cggcaggttc
    11221 agcgccgaca tcatgcgatc gaccgactca acggtcgcag catcgccttt gtgataagcg
    11281 attaacgtgc gtgccagcgg ccccacttcc atcgcgttac cgcgccagcg cggcgctttg
    11341 atccacgagt agcgttcctg ttcattcagc tgctgaatgt tggtatcgct gcctttgaca
    11401 tcgccggggt tgtaccacgg gtcggtaatg ccatcgaatg gatgacgccc gacctgatcg
    11461 ttgggataac gataccaggc gtggtcgaca aactcctgca cctgctgcgg atcaaccaaa
    11521 tccactggca gcacattgtt gaagtcgccg ttaatcaccg cgccgccagg catcagcaga
    11581 cttttctcac caaagtcgtt ggcaatatcc gggaacgcac cgtagctgag gacacattta
    11641 tcagaaagac ccgtgccgat ttcgctccac ggtttgttga actgaccgat ggctaaggcg
    11701 tcggggatca tcacgttgtt aatgaagtcc gccgtgcggg tgataatcga ctgcaccagg
    11761 ttcaggcgtt ccatattgac cgccccgacc gcgccgcttt cgtcaatgtt aatggcgcaa
    11821 ggcatcccgc cgacaatcca gtttggatgc gggtttttac cgccaaagac cgcgtggatt
    11881 ttgacaattt cacgctggaa atcgagagct tcgagatagt gggcaaagcc catcaggtta
    11941 gcttctggcg gcagtttgta ctgcgggtgc ccccagtagc cattgcggaa gatccccaac
    12001 tgcccgcctt caacaaattt tttcaggcgg ttttgtacgt cgaagaaata gccaggggat
    12061 gatttcggcc aagaggagag actttgcgcc agttcggagg ttttccgcgg gtcggctttc
    12121 agcgcatcta acacatcgat ccagtccatc ccggcaagct gatagaagtg caccagatga
    12181 tcgtggcacc agagcgttgc cagcataatg ttgcggatga tattggcgtt gtccggcact
    12241 ttaataccga tagcatcttc gatggcgtaa accgaagcca gggcgtgtac gccagtacag
    12301 acgccgcaga tacgttcaac gaacgcccac gcatcgcgcg ggtcgcgccc ttgtaggatg
    12361 atctccagcc cgcgaaacat ggtgccgcag gagacggcat tggtgatcac attctgatcg
    12421 ttaatattca cttcgcagcg catgtggcct tcgatgcgcg taatcgggtc gaccaccagg
    12481 cggcgtccgg cattattgat ggtgtatccc tgagtttcgt actgagtgct catgcctgtt
    12541 tatcctcatt gcctggctga tgttcggttt ctgtaggttg ctggttatga cgtctgcgct
    12601 ggtcaacggc gctggcgact gcgtgcacac caacagccgc tgccaccaca ccaagcgcgg
    12661 ttaaaccgac ggtatcggcg gtggaatgag tacccatttg cggaatatcg accacgcggc
    12721 tgtagaacga accgcaatcc cagaaaccat tttccgcaca gcccaggcag ccgtgaccag
    12781 actggattgg gaaagaaacg ccatcattcc agcgtgtgga ggaacaggcg ttataggtgg
    12841 taggcccttt gcagcccatt ttgtacaggc agtaaccttt gcgggcagcg tcatcatccc
    12901 aactctggac gaactctccg gcgtcgaagt gggcgcggcg atagcattta tcgtggattc
    12961 gctgaccata gaacatcagc ggacggccca ttctgtcgac atctggcaag cgatcaaagg
    13021 tcaccatgta agtaatgatg gcgctcatca catccgggat cggcgggcag ccaggtactt
    13081 tgataatggg tttgtcggtg atgactttgt cgataggcgt tgcctgcgtc ggattgggcc
    13141 acgcggcctg cacgcagccc caggacgcgc aggttcccca ggcgataatc gcgctggctc
    13201 cggcagcggc acgtttgagt ttctcaataa acggtcgacc gctgctgata cagaacatcc
    13261 cctgctcgcc cagcggcgga ttaccttcta ctgcgaggat atatttgcca ttgtattgcg
    13321 tgatgatgtc ttcgaagact tcttccgcct gggttccggc ggcagccatc aaagtatcgt
    13381 cataatcgag ggaaatcagg gaaaggatga cgtccttcgc cagtgggtga gcggagcgga
    13441 taaaagattc ggtacagcag gtgcattcca gaccgtggat ccataccacc ggaatgcggg
    13501 gtttgttctc cagcgcccag gcaatctttg gtgccattcc cgcgcctaat cccagcgacg
    13561 tggcagccag actacaatat ttgagaaagc tgcgccgggt aacgccctga cgccgcatgg
    13621 cctggtaaaa tgtttcctcg ttattcatat cgcacgtctc ttctccttgc gacaccggca
    13681 ggacaccggg cgcttatggt cggcaagcga cgtccctgta gcaaggcaaa ccatcgcttc
    13741 acgtgagatg ctgaaaacga aagctcatcc ttctgcactg gcgcacgtcg ccagaaagta
    13801 ttgttaataa agcgtagtga aa
//
LOCUS       DAZPJP010000104        13531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPJP010000104 DAZPJP010000000
VERSION     DAZPJP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.104"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHU9J_002714"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHU9J_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1906211.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHU9J_002715"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHU9J_002715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1906212.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHU9J_002716"
     CDS             complement(2203..3618)
                     /locus_tag="ACHU9J_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1906213.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHU9J_002717"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHU9J_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1906214.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHU9J_002718"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHU9J_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1906215.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHU9J_002719"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHU9J_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1906216.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHU9J_002720"
     CDS             11339..11395
                     /locus_tag="ACHU9J_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1906217.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHU9J_002721"
     CDS             11666..11722
                     /locus_tag="ACHU9J_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1906218.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHU9J_002722"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHU9J_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1906219.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHU9J_002723"
     CDS             12275..12934
                     /locus_tag="ACHU9J_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1906220.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..>13531
                     /locus_tag="ACHU9J_002724"
     CDS             12931..>13531
                     /locus_tag="ACHU9J_002724"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFR1906221.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSP"
BASE COUNT         3165 a         3714 c         3679 g         2973 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc cagggggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgcaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgactgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgccgctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcgtc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct c
//
LOCUS       DAZPJP010000105        13319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPJP010000105 DAZPJP010000000
VERSION     DAZPJP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.105"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..160
                     /locus_tag="ACHU9J_002725"
     CDS             <1..160
                     /locus_tag="ACHU9J_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HFR1906222.1"
                     /translation="VPFFTPFFNGLASATQEQLDLLPELLKQFLSSPAVNERTDDDKT
                     LALALWLP"
     gene            157..2097
                     /gene="yegI"
                     /locus_tag="ACHU9J_002726"
     CDS             157..2097
                     /gene="yegI"
                     /locus_tag="ACHU9J_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1906223.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(2110..3462)
                     /gene="yegD"
                     /locus_tag="ACHU9J_002727"
     CDS             complement(2110..3462)
                     /gene="yegD"
                     /locus_tag="ACHU9J_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1906224.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3556..4404
                     /gene="alkA"
                     /locus_tag="ACHU9J_002728"
     CDS             3556..4404
                     /gene="alkA"
                     /locus_tag="ACHU9J_002728"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1906225.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(4514..7831)
                     /locus_tag="ACHU9J_002729"
     CDS             complement(4514..7831)
                     /locus_tag="ACHU9J_002729"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1906226.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGILVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            8149..8790
                     /gene="udk"
                     /locus_tag="ACHU9J_002730"
     CDS             8149..8790
                     /gene="udk"
                     /locus_tag="ACHU9J_002730"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1906227.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8882..9463
                     /gene="dcd"
                     /locus_tag="ACHU9J_002731"
     CDS             8882..9463
                     /gene="dcd"
                     /locus_tag="ACHU9J_002731"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1906228.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9485..11338
                     /gene="asmA"
                     /locus_tag="ACHU9J_002732"
     CDS             9485..11338
                     /gene="asmA"
                     /locus_tag="ACHU9J_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1906229.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11612..13195)
                     /locus_tag="ACHU9J_002733"
     CDS             complement(11612..13195)
                     /locus_tag="ACHU9J_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1906230.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         3199 a         3470 c         3542 g         3108 t
ORIGIN      
        1 tgtgcctttc tttaccccgt ttttcaatgg actggcgtca gcaacgcagg aacaactcga
       61 tttattacct gaattgttaa agcagttttt gtccagcccg gcggtaaacg agcgtacgga
      121 tgatgataaa acgttggcac tggcgctgtg gttaccatga aacccacttt atatactgct
      181 actggtgagt gcgttacgcc aggccgtgaa ctgggcaaag gcggtgaagg cgcggtttat
      241 gatatcaatg agtttgtcga tagcgtcgcc aagatttatc acacgccgcc acccgcctta
      301 aaacaggaca aacttgcctt tatggctgcg acagctgacg cgcaattgtt gaattatgtc
      361 gcctggccgc aggcaacgct tcacggtgga cgaggcggaa aagttatcgg ttttatgatg
      421 ccaaaagttt ctggtaaaga accgattcat atgatctata gcccggcaca tcgtcgtcaa
      481 cgttaccctc attgtgcgtg ggattttcta ctctatgttg cgcgcaatat tgcttcatct
      541 tttgctacgg ttcacgagca cgggcacgtc gtgggtgacg taaaccagaa cagttttatg
      601 gtaggtcgcg acagcaaagt ggtgttgatt gatagcgact cctttcagat taacgccaat
      661 ggcacactgc atttatgcga agtcggcgtg tcgcatttta cgccgccaga gctacaaacc
      721 ttgtcgtcat ttgtcggctt tgaacgcaca gaaaatcacg ataattttgg ccttgcgttg
      781 ctgatttttc acgtcttgtt tggtggccgg catccttatt ctggtgtgcc gcttatctct
      841 gatgcgggta atgcgctgga gacggatatt acccatttcc gttatgccta cgcgtcagat
      901 aatcagcgac gtggtttaaa accgccgcca cgatcgattc cgctatcgat gttaccgagt
      961 gatgttgaag ccatgtttca gcaggcgttt acggaaagtg gcgtggcaac cgggcgtccg
     1021 acggctaaag cgtgggtatc agcactggat tctctacgcc aacagttaaa gaaatgtacc
     1081 gtttcggcaa tgcatgttta tcccggtcat ttggctgact gcccatggtg tgcgctggat
     1141 aatcaaggtg ttatctattt tattgatctc ggcgaagagg tcattaccac cggcggggat
     1201 tttgtgctgg caaaagtctg ggcgatggtg atggcgtcag tagcaccgcc agcattgcaa
     1261 ctgccattac ccgatcattt ccaaccgact ggcaggccgc ttcctttagg cctgttacgg
     1321 cgcgaataca tcattctgct tgagatcgca ctgtcagcat tatcgctgct gctttgcggc
     1381 cttcaggcag aaccgcgtta tattattttg gttcctgtgc tggcggctat ctggattatt
     1441 ggcagtttga caagcaaagc gtacaaagca gaagttcagc aacgccgtga ggcatttaat
     1501 cgtgcgaaaa tggactatga ccatttagtc cgccagatcc agcaggtagg cggcctggaa
     1561 ggttttatcg ccaaacggac gatgctcgaa aaaatgaagg acgaaattct cgggttaccg
     1621 gaagaagaga aacgcgctct ggcagcactt catgacactg caagggaacg gcagaagcag
     1681 aagtttctgg agggattttt tattgatgtt gcctctattc ccggcgttgg ccctgcgcgt
     1741 aaagcggcgt tacggtcttt tggtattgaa acagccgcag atgttacccg tcgtggcgtt
     1801 aagcaagtga aagggtttgg cgaccatctg acccaggcgg ttatcgactg gaaagcgaac
     1861 tgtgaacgcc gttttgtgtt caggccgaat gaagcgataa cgccagcaga cagacaagcg
     1921 gtaatggcga aaatgaccgc caaacgacat cggctggaat cggcgttgac agtcggcgcg
     1981 acagagttgc agcgattccg ccttcatgct ccagcacgga ccatgccgtt gatggaaccg
     2041 ttacgtcagg cagcagaaaa actggctcag gcgcaggcag atttaagccg ctgctgagcc
     2101 atttttcaat taacgaaaca ccacttccgc ccagcgtgcc agcccggcgg tgacggagcc
     2161 aaagtcatcg ccgcctgcaa tcggaatgcc cggcaactgt tctgccagcg cttttttaat
     2221 ctgcggagaa cgggcgctac cgccggtcag gtagataacg tccggttttt cctgggcgtt
     2281 atccagggcc agttgcacct gttccagaat ccgcgccagt ggctggctga gggcgctttc
     2341 cagccctcgc tggctaatca gcgtagccag ttcattgctg ataaacggca gtgaggcgcg
     2401 ggtttctgct acgcttgaaa gagcaatttt gcactcttct gcgctacgca ccaggcgata
     2461 gcttaaacgc tgacgccaga ctttctgtaa cagggccact ttttccggtt cgcgggcatc
     2521 gcgtaccaga tcgttaagca gacgaccgtt ggcactactg tagaaatcac tctgcgcagg
     2581 tacgtcgttg atggcaaccg cattccacca cggcaggatc ggcagggcga tgcctttttc
     2641 ggtttcgcca cccataccca gcagtggcat caggttttta aacgccaggg cgatatccag
     2701 atcgttaccg ccaatacgac aaccactgtg acccagcaga ctggcttcac gatcgagacg
     2761 cgaacgccac tgcggcccca tcagcagcaa tgaacagtca gtcgtaccac cgccgatatc
     2821 caccaccagc acccgttttt cttcctgcaa ggtggcttcg taatccagcc cagccgcgat
     2881 cggctcgtac tggaatacca cgtccctgaa tccggcacgc ttcgccgcgc gttccagaat
     2941 ctcttgcgcc tgggcgtttg cttcatcgcc gcccagcccc tggaagttga tcgggcgacc
     3001 aatcaccgcc tgagtaatcg cttctggcag ctgcgcctgc gcctgctggc gaatgtgcaa
     3061 catcattgcg cagaccagat cctcaaacag cgctacctgc tgcggtttta agccgctggc
     3121 accgaggaac gattttggtg atttcacaaa ccacacttct tctggatcat caatgtactg
     3181 tgccagtgag gaaagaccga actgcacgct tttcgccgta acatcgatat cttcttcgcg
     3241 gttataacga atcgcccgac gtaacagcgc ctgcgtttca tcgtcgtctg ccggaacatc
     3301 atgatggcgg tacagccatt cgcttaccga ttcacgcgtt ggcgcgcaaa gcattgaagg
     3361 cagcagcgtg ctgtcgtttt ccattttcag taattgcggt ttaccgtcac gcatgaccgc
     3421 cactgaacag tttgctgtac cgtaatcaaa accaataaac acgaattaat ccccatgccg
     3481 gtgaagaagg ggcgtgactt tagcgaaatg ttgctgtcgc gacaaccgga aaaagcggat
     3541 taacaaggag atgcgatgta taccctgaac tggcagccgc cgtatgactg gtcgtggatg
     3601 ttgggatttc tcgccgcccg tgcggtgagc ggtgtggaaa cggtcgcgga caattattat
     3661 gcccgtagtc tggcggtggg cgaatatcgc ggcgtagtga ctgctattcc ggatatagcc
     3721 cgccatactc tgcacataaa tttaagtgca ggtttagaac ctgttgccgc agagtgtctg
     3781 gcgaaaatga gccgcctgct tgatctgcaa tgtaacccgc agattgttaa cggtgcgttg
     3841 ggaaagttag gcgcggggcg gcccggattg cgtttacccg gctgtattga tgcttttgag
     3901 cagggtgtgc gggcgatttt aggccaactg gtgagcgtgg cgatggcggc aaaattgact
     3961 gccaaagtgg tacagcttta tggcgaacgg ctggatgatt ttccggagta tatctgcttc
     4021 cccacgcctc agcggctggc agcagccgac ccgcaggcat taaaagcgtt aggcatgccg
     4081 ttgaaacggg cagaggcact gattcatctg gcaaatgcgg cgctggaggg cagcttacca
     4141 atgacaattc cgggcgatgt ggagcaggcg atgaaaacgc tgcaaacttt tccgggtatc
     4201 gggcgctgga cggcgaatta ttttgctttg cgtggctggc aggcgaaaga tgtttttctg
     4261 ccggatgatt atctgattaa acggcgattt ccgggaatga caccggcaca aatccgccgt
     4321 tatgccgagc gctggaagcc ctggcgttct tatgcgctgt tgcatatctg gtatacggaa
     4381 ggctggcaac cagacgaagc atgacgttgg tcgccggatg cggtgcgagc gccttatccg
     4441 gtctggggaa tgagcgattt gtaggcatga taagacgcgt tagcgtcgca tcaggcaatg
     4501 ggcaaacgct ctctcagtta atcgcgaaat aactgctatt caccagcaaa tccagcggtt
     4561 gggcatcggc aatcacatca ccataaatca gatcgacgcc gatgccagaa agcgtatcca
     4621 tcactaaggg taaaacgact ggcccggcga tggttttcat cccgagtcgc tgagcgtgcc
     4681 cctgaataat cgtaatcagc atctcatcca tcaaattacc ctgcacgttg gcgcataact
     4741 caccatcaag tagcaggtaa tctgccatat tcgctttcag cgagttgaag atttgcagat
     4801 cgcggcccac ctggctgagc actatccgac atcccgccag ctgcagtttt tgcacgcttt
     4861 ctgcgtgatc taaaatcgct tcagccggaa taatcagatg taataaccgt ggtggtagag
     4921 ggctattttc cagctgctca agcagatcat tcaccagcgt ggcgctactc aaaccggcaa
     4981 cggaaagggg gagggcgatg cttatgcctt tactggcaac cgctttggcg gcctgctgga
     5041 aaaattcgtg gaatacccgt cggtcaagag catggctaag tgccggatcg ctgaagctac
     5101 gacgaaatgt ttgttcatca ataatttcgc cttcgcaact ccagagctta agcgaaatca
     5161 gccacaaatt acgcgcttcc gggatccgcg gcgaagcgac accgtgggcg atcatcatca
     5221 actgattctc tttaatcatc cgccactgtt catcaagcga catcgccgcc cgttcgctat
     5281 gtgcggcagc ttgctgtggt tcgtaaaccg tcacccggcc ccgtccacca tttttggagg
     5341 cataacaggc gatatcagcc tgcgacatca cttctgccgc ctgatgattg ttgtcatcga
     5401 tcaaggtaat cccggcgctg gcacctaccc gatgtacccg gccttcccat ataaagtgat
     5461 agtcattcac agcactgata atacgtgtag cgataaaacg agcgctttca acattgcagt
     5521 ctggcagcag cagaccaaat tcatcaccgc cgagccgcgc cagcacgtca ctggagcgca
     5581 gcatactcag catcaacgac gccagttcgc gcagcaaagc gtcgcccgcc gcatgcccgg
     5641 cgctgtcatt caccgcttta aagcgatcaa gatcgataaa caccagggca tgtcgctgat
     5701 gcgtactgtt taccgtttgc agcaggatac gcagttgttt ctcaaaactg gcgcggttgg
     5761 cgagatgcgt cagtgcatca tgggaggcgc tgtagctcag ctggcgcagc attttgcgtg
     5821 attcggtgac gtcctgaatc accagaaccg aaccaatatt gctgccgtcc agagtactta
     5881 acggcgtaat actgtaatgc acgtcgtaac tgccgccgct ccgacagtgc aacaccacat
     5941 cttgttcgat tgcggaacgt gaggtgtcgg cactgtaaat gttctccatt aatggtccgt
     6001 tgtcgccaaa agtaatatgc aacaccgtca ggagcggaac acctaacgct tcttcctgcg
     6061 tccagccgct catcttctcc gccactggat tcataaaggt aattttcatc gccatatcaa
     6121 tacagaccac ggcttcaccg atggaatcaa gcgtaatgtg caggcgctct ttttcctgaa
     6181 acaatgcctc gttaagctgt ttcacttcgg tcatatccat attgatgccg aggagacgtt
     6241 cgacttcgcc ttctttattc agtacccggt tggcgagggc gcggatatgg cgaataccgt
     6301 cttttacggt aatgcgaaat tccagtttaa agggcgagcg tgattgcaac gaatcacgaa
     6361 tcactttttc ggcatgctgg cgatcttccg gcagcacgca ctcgtaccac acctgccagt
     6421 tcggtttgat atgtggagga atttcataca gctcgaacat ccgcttatcc cagctaaaaa
     6481 cattcggctt caactcccac tcccagatgc caatcccgcc cgcttcgtta gccagcgtga
     6541 tgcgctccat cagttgctga ttgacctgtt cggtgcgttt tagctcgtta atgtcttcaa
     6601 tctgagcgat aaaatagagc ggcgtgccat ccgtgtggcg caccagtgag acggcaagca
     6661 acgcccagac aacatcgcca ttgcggttgt agtagcgttt ttccattgaa taggtgttta
     6721 tttcaccgct tatcagcttt tcaacctgtt ggagatcttt attgagatcc tccggccagg
     6781 tcagttgctg aaaggtgagt ccgcgcagct cttcctgact gtacccgaga aactggcaga
     6841 gcgctttgtt ggtttgcagc cattgtccct cggtgcccac taacgccata ccgatagcgg
     6901 aatattccat cgcgttccga aagcgggttt cgctttcgga aatgtgtttg cgttccgcac
     6961 gaaaggcata catcaccatg gtcatgatgt tggcgggcag caggatcagc aaaaacggta
     7021 gccacggcat atggctcatc aggtacgtac gcggcgtagc aagcagggag ggatccgcgg
     7081 ccatcatcag cgacaccatc atcaccgtgg taaggaagat caaaaaggct tccattcgtg
     7141 gcaggcgcac ggcgctccac atcaacagca caataatgaa agtaaaaggc cacggcagat
     7201 acagcatcga aagccagctt aacgtcagtg tgatggctaa cgtgagcagc gactcaaaaa
     7261 gcaaccgtgg gttgcgatgg cgcagcagat agtgtggttt aaataacaat cccagcggca
     7321 ccagtgccag agcgccgatg gattctgaca gtacccatat caaaaatgcc ctgagaggat
     7381 cgtctccggg cgtcagcagg acaaccagaa taccccccaa cagaggcgga acaatggcgc
     7441 tgccgagtgc cagacgcagc cagtcagcca gattttgcaa ggggttatac cacggcagca
     7501 atttacgtag cagcactgcc ccgaccacgg cttcaacaat attgatggtc gtccaggtca
     7561 tgttcagcga gctggtggaa aaaagcagga tggatgcggc gatatttccc agcgaacagc
     7621 tcagcgcaat tcccggccac atgcgcccgg catggcgata aaacgccacc atcatgatgg
     7681 acgtcgggaa ccacagtggg gcgagttggg tgccaaactg cgaaagctca agcgagaaaa
     7741 gggtaaagat aaacgagact aaacctaaac tgaccaggtg aagcagcggg tggggcaggg
     7801 caattaatac atgctgtgat tgtttgctca ttaccgcttt atccgcaaga ccaggtcgcc
     7861 agtattcgtt tctgtgttta tgctagtaca aaagttttac attttatatg cgaattgctc
     7921 ataaagtgac gtaaaggcgg ataatttgcg caactgcgta taacattttt taccttacat
     7981 aaaactgatc aacgtaattt gccaccggga attctttgtc aacaattaac tctgagcgcg
     8041 cggcaacgct attcgactgg tatcagacgg atgaaatccc tataattgcc gcgtttggcg
     8101 cttcgtcgcc cccttcctaa catccaggtt aatcaggtcg ctaaatatat gactgatcag
     8161 tctcatcagt gcgtcattat cggtatcgct ggcgcatcgg cttccggcaa gagtcttatt
     8221 gccagtaccc tttatcgtga attacgtgag caagtcggtg atgaacacat cggcgtaatt
     8281 cccgaagact gctattacaa agatcaaagc catctgtcga tggaagaacg cgttaagacc
     8341 aactacgacc atcccagcgc gatggatcac agcctgctgc ttgagcattt acaagcgttg
     8401 aaacgcggct cggcaattga cctgccggtt tacagctatg ttgaacatac gcgtatgaaa
     8461 gaaacggtga cggttgagcc gaagaaggtc atcattctcg aaggcatttt gttgctgacg
     8521 gatgcgcgtt tgcgtgacga acttaacttc tccattttcg ttgatacccc gctggatatc
     8581 tgcctgatgc gccgcatcaa gcgtgacgtt aacgagcgtg ggcgttcaat ggattccgtg
     8641 atggcgcaat atcaaaaaac cgtgcgcccg atgttcctgc aattcattga gccttctaaa
     8701 caatatgcgg acattatcgt gccgcgcggc gggaaaaacc gcatcgcgat cgatatattg
     8761 aaagcgaaaa taagtcagtt ctttgaataa gcttgataaa ttgtgtaccg ttcagtgata
     8821 acctggtatg cccttgacgt aattggcgtt aagggagtga tgcgcgaaag gagaaaatgc
     8881 catgcgtctg tgtgaccgag atattgaagc ctggcttgat gaaggccgtt tgtcgatcaa
     8941 cccacgtccg ccagtggagc gtattaacgg cgcgacggtg gatgtacgcc tgggcaataa
     9001 atttcgtacc ttccgtggtc acacggcagc gtttatcgat ctgagcggtc ccaaagatga
     9061 agtgagcgcc gcgcttgacc gcgtgatgag cgatgagatc gttctcgacg agggcgaggc
     9121 gttctatctt cacccaggag agctggcgct ggcggtgacg ctggagtcgg tgacgctgcc
     9181 agccgatctg gtgggctggc tggacgggcg ttcctctttg gcgcgtctgg ggctgatggt
     9241 gcacgtcacc gcgcaccgta tcgatccggg ctggtctggt tgcattgtgc tggagttcta
     9301 caactccggt aagctgccgc tggcgctgcg tccgggcatg ttaattggtg cgctgagctt
     9361 tgagccgctt tccggcccgg cggcgcgacc ttacaaccgc cgtgaagatg cgaaatatcg
     9421 caaccagcag ggcgcggtag ccagccgaat cgataaagac taattcaggc ccattgagga
     9481 taccatgaga cgatttctga cgacgctgat gatactcctg gtcgtgctgg tggccgggtt
     9541 atctgcgtta gtgttgctgg tgaatccgaa tgatttccgc gactatatgg tcaagcaagt
     9601 tgctgcacgt agcggttatc aattgcagct cgacgggcca ctgcgttggc acgtctggcc
     9661 gcagcttagt atcctctccg ggcgaatgtc tctcaccgcc cagggcgcaa gccagccact
     9721 ggttcgcgcc gacaacatgc gtctggacgt ggcgctttta ccactactga gtcatcaact
     9781 gagcgttaag caggtgatgc taaaaggggc agtgatccaa ctgacgccgc agacggaagc
     9841 ggtgcgcagt gaagacgctc cggttgcacc gcgcgacaat accttgccgg atctgtcaga
     9901 cgatcgcgga tggtcgtttg atatatccag tcttaaggtg gcggacagcg tgctggtgtt
     9961 ccagcatgaa gatgacgagc aggtgacaat ccgcaatatc cgcctgcaaa tggaacaaga
    10021 tccccaacat cgtggctcat ttgagttctc cgggcgggtt aatcgcgatc agcgcgatct
    10081 cacaatatcc cttaacggta cggtggatgc ttctgattat ccgcatgatt taacggcggc
    10141 tattgaacaa attaactggc agttgcaggg tgccgattta ccaaaacaag gtattcaggg
    10201 gcaggggagt ttccaggccc agtggcagga gtcacataaa cgcctttcat ttaaccaaat
    10261 cagcttgacc gccaatgaca gtacgctgag cgggcaagca caggtcacgc tgacagagaa
    10321 accggaatgg cagctgagac tgcaattccc gcaactgaat cttgacaacc tcatcccgct
    10381 taatgaaacc gcgaatggtg aaaacggtgc cgcgcagcag gggcagagcc aatcaacgtt
    10441 gccgcgcccg gtcatttctt cgcgtattga tgaaccggcc tatcagggac tgcaaggctt
    10501 tacggctgat attttgttgc aggccagtaa cgtgcgctgg cgcggaatga attttacaga
    10561 tgttgccacg caaatgacca acaagtcggg tttgctggaa attactcaac tgcagggcaa
    10621 acttaacggt ggacaggttt cactgccggg cacgctggac gcgacatcaa taaatccgcg
    10681 gataaacttc cagccacggc tggaaaacgt tgagattggt accattctga aggcgtttaa
    10741 ctatccgatt tcgttgaccg gaaaaatgtc actggctggt gatttctccg gtgctgacat
    10801 agatgccgac gcattccgcc acaactggca aggacaggca catgtcgaaa tgaccgacac
    10861 gcgcatggaa gggatgaact tccagcagat gattcagcaa gcggtagagc gtaatggcgg
    10921 tgatgtgaag gccgctgaaa actttgataa cgtgacgcgg cttgaccgct ttaccaccga
    10981 tttgacgttg aaggatggcg tcgtgacgtt aaacgacatg caaggtcaat cgcctgtgct
    11041 ggcgctgaca ggggaaggca tgttgaatct ggcagatcaa acctgcgata cccagtttga
    11101 tattcgggtc gtgggtggct ggaacgggga aagtaaactg attgatttcc tgaaagaaac
    11161 gccagtaccg ctgcgggttt atggcaactg gcagcaactc aattacagcc tgcaagtgga
    11221 tcagttactg cgcaaacatc tacaggacga agcgaaacgt cgcctgaatg actgggccga
    11281 gcggaataaa gattcccgca atggcaagga tgtgaagaag ttgctggaga agatgtaggt
    11341 taccaggtga ccagtcctgt aggcctgata agacgcgtaa agcgtcgcat caggcgttcg
    11401 tggcggcatc attatattct gaatatgtaa gatcgaaggc tgttccgttc acttgatgtt
    11461 cctcgctact atggaacgct caagccggtg aacgtgccag ggtggctcaa tcgccaccac
    11521 cctggcaacc cgggctcccg gcggtaaatc gccgctggcg cggttccctc atcttattcc
    11581 tttcggctat tgggtaaaat gaccgatacc atcacacctc gtactccaac tcgccatcct
    11641 tatgcagcgg aataatctgc accttctgca cgcgatggct ttccacctgc aacgttttaa
    11701 gcaaataatc ccccacctga acttcttcgc caggttttgg aatacgctgt aaatattcca
    11761 tcaacagccc ggcaatggtg tgatattcac gtttttcatc cagcggcagc ggcacatatt
    11821 gcaccagatc ttccagcggc atatgaccat tcgccgtcca ggaaccgtcc gcattcttct
    11881 gaatatcatg gcgggcgtcg atctcttcca cttcgttcgg taagttaccg acaatggttt
    11941 cagtgacgtc acttaatgtc acaatccctt ccaccgagcc aaactcatcc accacaaaag
    12001 caaagtgcgt gcgggcatta cggaactgct ccagggcagg taacaacggc aaggtttccg
    12061 ggaacaccag cggctggcga atcaacaccc gcaggttgag cggttcgccg cgcagagact
    12121 gttgtagcag gtcgataacg tgaacaacac cgagcaaatc ttctgcgtca tcaccgtcgg
    12181 taaccaccag ccgcgtatgc tgatttcgct ccagcaactg gcggatttct tcttccggcg
    12241 cgttgagatc gatatgctca atatcgtggc gcgacgtcat aatgctgctg acggtacgct
    12301 ggttaagatt aagtacccgc tcaatcatcc gccgttcctg cggattaaag atctgctggt
    12361 taccatgatc caccagcatg gacgcggttt cggcgtccag ttccgcatct tctttttgcc
    12421 cgctaagcag gcgcattacc gcttcggtag tccgctggcg cagcgtctgg ttagcggaaa
    12481 gaaaacgtcg ccggttaaag atagccaact gattgagcgc ctcgatcatc accgagaaac
    12541 caatggcagc gtacaggtag cctttcggaa tgacgaagcc gaaaccttct gccaccaggc
    12601 taaagccaat catcaacagg aagcttaagc agagaataac gatcgtcggg tgactgttaa
    12661 caaattgcgt taacggcttg ctggccatca acatcaggct gatagcgata accacggcgg
    12721 ccatcatgac cagtaaatgg tcgaccatcc cgacggcggt aatcactgag tcgagtgaga
    12781 aaatggcgtc cagtaccaca atttgcgtca ccacgcccca gaacttcgca cctttgcgtt
    12841 gtgtgggatt attgctgtct ttcccttcca gccgttcgtt cagctccatc gtggctttga
    12901 acaacaggaa gaaaccacca aacagcatga ttaagtcgcg ggcgctaaag gtaaacgagc
    12961 ggaagctgaa cagcggttga gtcagggtga ccagccagga gattgacgcc agcagtaaca
    13021 ggcgcattaa catcgccagc agcagcccgg taacccgtgc gcggtcgcgc tgcctcggcg
    13081 gtagtttttc ggcgaggatg gcaataaaga ccaggttatc aatgccgagg accagttcga
    13141 tcacaatcag cgtgattaac ccggcccaga tagacggatc ggcaatccat tccatagtaa
    13201 gtacaaaacc tattcgttgt atgacaaatg tcacaattcg atcatggata aatcggggca
    13261 aaaatgcaat gttgcagcac tatttatctc atattatttg agggtaataa cctcacatt
//
LOCUS       DAZPJP010000106        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPJP010000106 DAZPJP010000000
VERSION     DAZPJP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.106"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHU9J_002734"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHU9J_002734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1906231.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHU9J_002735"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHU9J_002735"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1906232.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHU9J_002736"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHU9J_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1906233.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHU9J_002737"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHU9J_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1906234.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHU9J_002738"
     CDS             complement(4036..5568)
                     /locus_tag="ACHU9J_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1906235.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHU9J_002739"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHU9J_002739"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1906236.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHU9J_002740"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHU9J_002740"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1906237.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHU9J_002741"
     CDS             complement(8663..10399)
                     /locus_tag="ACHU9J_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1906238.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSASWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHU9J_002742"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHU9J_002742"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1906239.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHU9J_002743"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHU9J_002743"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1906240.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHU9J_002744"
     CDS             complement(12121..12855)
                     /locus_tag="ACHU9J_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1906241.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHU9J_002745"
     CDS             13056..>13260
                     /locus_tag="ACHU9J_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1906242.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3366 a         3371 c         3488 g         3035 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact ggcactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacta
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZPJP010000107        13135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPJP010000107 DAZPJP010000000
VERSION     DAZPJP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.107"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(597..1688)
                     /gene="ychF"
                     /locus_tag="ACHU9J_002746"
     CDS             complement(597..1688)
                     /gene="ychF"
                     /locus_tag="ACHU9J_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1906243.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1805..2389)
                     /gene="pth"
                     /locus_tag="ACHU9J_002747"
     CDS             complement(1805..2389)
                     /gene="pth"
                     /locus_tag="ACHU9J_002747"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1906244.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2666..2944
                     /gene="ychH"
                     /locus_tag="ACHU9J_002748"
     CDS             2666..2944
                     /gene="ychH"
                     /locus_tag="ACHU9J_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1906245.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2999..4678)
                     /gene="dauA"
                     /locus_tag="ACHU9J_002749"
     CDS             complement(2999..4678)
                     /gene="dauA"
                     /locus_tag="ACHU9J_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1906246.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4802..5749)
                     /gene="prs"
                     /locus_tag="ACHU9J_002750"
     CDS             complement(4802..5749)
                     /gene="prs"
                     /locus_tag="ACHU9J_002750"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1906247.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5900..6751)
                     /gene="ispE"
                     /locus_tag="ACHU9J_002751"
     CDS             complement(5900..6751)
                     /gene="ispE"
                     /locus_tag="ACHU9J_002751"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1906248.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6751..7374)
                     /gene="lolB"
                     /locus_tag="ACHU9J_002752"
     CDS             complement(6751..7374)
                     /gene="lolB"
                     /locus_tag="ACHU9J_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1906249.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7588..8844
                     /gene="hemA"
                     /locus_tag="ACHU9J_002753"
     CDS             7588..8844
                     /gene="hemA"
                     /locus_tag="ACHU9J_002753"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1906250.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8886..9968
                     /gene="prfA"
                     /locus_tag="ACHU9J_002754"
     CDS             8886..9968
                     /gene="prfA"
                     /locus_tag="ACHU9J_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1906251.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9968..10801
                     /gene="prmC"
                     /locus_tag="ACHU9J_002755"
     CDS             9968..10801
                     /gene="prmC"
                     /locus_tag="ACHU9J_002755"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1906252.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10798..11190
                     /gene="sirB2"
                     /locus_tag="ACHU9J_002756"
     CDS             10798..11190
                     /gene="sirB2"
                     /locus_tag="ACHU9J_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1906253.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11194..12004
                     /gene="sirB1"
                     /locus_tag="ACHU9J_002757"
                     /pseudo
     CDS             11194..12004
                     /gene="sirB1"
                     /locus_tag="ACHU9J_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            12040..12894
                     /gene="kdsA"
                     /locus_tag="ACHU9J_002758"
     CDS             12040..12894
                     /gene="kdsA"
                     /locus_tag="ACHU9J_002758"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1906254.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13043..>13135)
                     /locus_tag="ACHU9J_002759"
     CDS             complement(13043..>13135)
                     /locus_tag="ACHU9J_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1906255.1"
                     /translation="FAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3296 a         3281 c         3391 g         3167 t
ORIGIN      
        1 ttactataaa tatggcaaaa atattacaac aaacagcggt taaaaaattt ggtgaagatt
       61 ttttgtaata atcctaatta gttatgggaa tttcgcttga tgtatctgca taacaaaaac
      121 aatatgcgcc atttttgcat tactaataag aaaaagcaaa aaacatcgat attcattttt
      181 ggtcaatggg ttttctggcc tatcgttgtt attgtgctac tggttatcat tttcatcttg
      241 ccaggatcgc acgtaatcac gttattcggg gactttatct gcgggatatt acccttcagg
      301 taagtccttt tctcgccagc atattggagc gtgatcaata ttgatcagct gtgaaggggg
      361 tgatgtgtag ttgaacaaaa acttacgtta tatcactact aatgagatat ccgtcatctg
      421 acactgatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg
      481 atgttacaag aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggatttt
      541 atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaata aattaattag
      601 acgttgaaaa ggaagttcat cacatcgcca tctttaacga tgtaatcttt accttctgca
      661 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
      721 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc
      781 tgcggcgcgg ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg
      841 aagtaagttt gcaggttcag cagcttataa ccgacacgga tcacacggtt caggcccggt
      901 tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg
      961 tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca
     1021 cgcacctggt caagatatgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
     1081 attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt
     1141 aaatccagcg cgcgcagcat acctgcgttt tccagctggg gcaggcattt ttctaggacc
     1201 gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
     1261 gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg
     1321 tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
     1381 acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg
     1441 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc
     1501 gtacgttgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
     1561 acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
     1621 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
     1681 aatcccatga tttaaatcac cttaatatct taataatcaa tctgttattg ctaacagatt
     1741 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
     1801 cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa
     1861 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
     1921 taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgcca ggatgaccga
     1981 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
     2041 gtccattgtg accaccatgg ccaccgccca atttaaattt ggcgacgcca ggaggcagat
     2101 ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg
     2161 ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt
     2221 cgcctccaag agtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag
     2281 cgcgcaaacg ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg
     2341 cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg ttttttgtcc
     2401 tgagtgtgta cataactggc gcgtagttta ctggttgcgg ccccgcttga caaaaaactg
     2461 cgtatcaaat gcagataacg taataattgc ctgagtggac tattagaaag tcaaggtgtt
     2521 caggcgttta tttgtaaagt tttgttgaaa taagggttgt aattgtgatc acgcccgcac
     2581 ataacccaca gggtgttgtc tatactttac acataaggaa gaggggtatt ccctgttaca
     2641 acccagaaag ttccggaggt gacatatgaa acgcaaaaac gcttcgttac tcggtaacgt
     2701 gctcatgggg ttggggctgg tggtaatggt ggtcggcgtg gggtattcaa tcctcaacca
     2761 gttaccacag tttaatatgc cccagtattt cgcacatggt gcagtgctaa gtattttcgt
     2821 cggtgccatt ctctggctgg cgggtgcccg tgttggcggg catgaacagg tgtgcgaccg
     2881 ttactggtgg gttcgtcact atgacaaacg ttgccgccgt agcgataatc gccgtcatag
     2941 ctaacaaata atgcagtttg ctgaccagtc agtttgcgct gactggtcaa tctcgtactt
     3001 ataaatccgc catcgccgcg cgacgattcg ggaagaacgc gaggcgtccc gggatcggtt
     3061 gaatgcctgc gcgagccata gtgcgcagtg gctggaattc cacgttgcac acgcgcagtt
     3121 cacatccttc gggcagacgc ttcacaaaac gctggaacgc atcaagacca ccagcatcaa
     3181 gtaccggaac ggcatcccac ttcagaatca caatccgttt gccttcaaga cgtgactcca
     3241 ggtccgtgaa caagccttca gcagcagcaa aaaacagcgg gccaataacg cgcagtacca
     3301 gaacatcgtc tggaacatct acgactaccg gtgccaggcg agtcatacgt gcgatacgac
     3361 gcataaacag cagcgatgcc agcacgatcc ccacgctgat ggcaataacc atatcaaaca
     3421 gcacggtcag cgacatgcac agcagcatga caatgatgtc atctttcggt gcatgacgca
     3481 gcaagtcgac cactttatgc gcttcactca tgttccacgc caccatcaac agcagggctg
     3541 ccatagcgga aagcggcagc caggagagca gcggtgccag taccagcagg gcaagaataa
     3601 ccagaataga gtggatcacc gccgagatag gggaagttgc cccggcacgg acgttagcgg
     3661 cagaacgcgc gatggcagct gtagcggtaa taccaccaaa gaacggagcg ataatattcc
     3721 ccagtccctg tccaaccagt tcgctgttcg ccttgtgttt cgtcccggtc ataccatcca
     3781 gtaccacggc gcagagcaga gattcgattg cgccgagcat tgccattgag aatgccgcag
     3841 gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc tgaattaggc agatcccacg
     3901 gcagcaccag ttgcggcagc agttgcggaa taccgttacc ctgagaacca tcggccagga
     3961 cgtagtggaa ttgcgaaccg atggtagcaa catgtccgcc gagcaggtta acaatcccca
     4021 tcaccgcgca accagccagc aaggccggaa ggtgaccggg taaacgaatg cccagacgcg
     4081 gccaaaaaac aagaatacct agcgtcacaa tgccaatggc agcatcaccc acattaatgg
     4141 tcggcagcgc cataaataat gcgccgactt tttgtagata atgttccggg acatgggcca
     4201 tttgcagacc gagaaaatct ttaatctgca tggtaccgat ggtgatcccg atacccgagg
     4261 tgaaacctaa ggtgacggaa accggaatat actcaatcag gcgaccaaag cgtgccagac
     4321 ccatcagaat caaaaagatc cccgacagca aggtcgcaac cagcagtcct gccagtccaa
     4381 actgttgcga cacgggatag agaattacca caaatgccgc agtcggaccg gaaacgctaa
     4441 agcgtgaccc acccgtcaga gcaatgacaa tccccgcaac agctgcggta tataaaccgt
     4501 actggggtgc cacaccacta ccaatagcca acgccatcgc cagcgggata gcaataatcc
     4561 cgacggttat cccggcaatc aggtcacggg taaaccgtgc ggcagtatat ttttctttcc
     4621 agcaagcgtc gatcagagcg cggaaaggca tcacatgtga ggaaaatatt ttgttcacaa
     4681 taatgtttca tccgtgagcg catcatctgt caactaaatg gcaggtgaag gaggcatagg
     4741 tcatacaaat ggatattaca gacaaaaaaa cccgccgcag cgggtctttg agcgggttcg
     4801 attagtgttc gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg
     4861 ccagcatacc tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca
     4921 gcggaatggt atcgcagaca acgacttcat caattacaga gttacgcagg ttgttcgccg
     4981 cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt
     5041 tcagagcttc agcagcttta cacagcgtac cgccagtgtc gatcatatca tcgaccagta
     5101 cgcagtcacg acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg
     5161 gacgacgttt gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg
     5221 cacgcacaac gccgccgatg tccggagaaa ccacaattgg gttatccaga ttcagctgca
     5281 gcatgtcttc cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac
     5341 cctgaatctg ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga
     5401 ggaagtctgc aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct
     5461 ggcgcgcata gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca
     5521 gggcatcaac cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact
     5581 ggatgatgaa aatatcacca ccgcgtacat tttcgttgat ttgtacactg acttcgccgt
     5641 cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt
     5701 gtgctagttc cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct
     5761 caggcatgcg tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgatcc
     5821 tttccaggcg gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc
     5881 gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacacctt
     5941 tcgccacaaa gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact
     6001 ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt
     6061 ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc
     6121 aatcattgct gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg
     6181 ggagttcagg atctttaaaa atcactggtg tcggaatact tacaccaggg tgcgccacca
     6241 gataccactt ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg
     6301 cggcatgccc ccgaacaaag acaggaacat ctgcgcccag cgtcagcccc atttccgcca
     6361 gctcatccat gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg
     6421 cattggatga accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat
     6481 tcgcaccgct tcccgtcgga agacgcccgc tgtctgccgc agttttcatc aacaaccgcg
     6541 ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa
     6601 tatccccatc gtcacgaagc tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca
     6661 gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa
     6721 gttttgccgg agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa
     6781 cttgatgcgt tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgcgtttt
     6841 ggtgtcataa ccaccataaa caaccttcca gtttttgcca ttctggctgt aggtaatttc
     6901 gctcaggcgg tactggtcgt ccagtttgta gtcggttgca tcacccggta aacctaaaat
     6961 ccattggcgc aagctgttga gcggaattgg cattccggtc aatttgccaa tcatctcttc
     7021 ggcgtcatcg gcggtataac gctgaccttt attgtcgact aactgcacgt tacccggttg
     7081 agcattcagc tccagttccg tgctgcccaa tgggttagtg agcagcagac ggtagcgatc
     7141 ctggccggtt tgctgccaga aaaagcgggc gtacactttt tgttggtcag aaatataagc
     7201 gaacgcgccg cgagtctgat actgattaag attgcgcacg tcttgctgat gctgacgcca
     7261 ttgtggcgaa tccgggcttt tgccaggacc tttgggcgtg gtaacggaac aggcagtgag
     7321 cacaagagca gccagcggta gcaggcggat aagacgaaaa tcgggcaggg gcatagtgat
     7381 gacaagtcct tgagatacgt tgcagttata acccttaatg ctagcgttac cgtccgctat
     7441 cgtctatgtt caagttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa
     7501 agggggcgct ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaaccata
     7561 tcaacgttgg tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa
     7621 acggcacctg tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg
     7681 cttgacagcc tgcttgcgca gccgatggtg cagggcggcg tggtgctgtc gacgtgcaac
     7741 cgcacggaac tttatcttag cgttgaagag caggataacc tgcaagaggc gttaatccgc
     7801 tggctttgcg attatcacaa tcttaatgaa gaagatctgc gtaaaagcct ctactggcat
     7861 caggataacg acgcggttag ccatttaatg cgtgttgcca gcggcctgga ttcactggtt
     7921 cttggggagc cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt
     7981 catatgaagg ccagcgaact ggaacgcatg ttccagaaat ctttctctgt agcgaaacgc
     8041 gttcgcactg aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg
     8101 ctggcgcggc agatctttga atcgctctct acggtcacag tgttgctggt aggcgcgggc
     8161 gaaaccatcg agctggtagc gcgtcatctg cgcgaacata aagtacagaa gatgattatc
     8221 gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg aagttggcgc ggaagtgatt
     8281 gccctgagtg agatcgacga acgtctgcgc gaagccgata tcatcatcag ttccaccgcc
     8341 agcccgttac cgattatcgg gaaaggcatg gtggagcgcg cattaaaaag tcgtcgtaac
     8401 caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa
     8461 ctggcgaatg cttatcttta tagtgtggac gatctgcaaa gcatcatttc gcacaacctg
     8521 gcgcagcgta aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa
     8581 tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag
     8641 gcagagcacg ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc
     8701 gacgcgcaag ccattatgca ggatctggca tggaaactga ctaaccgctt gatccatgcg
     8761 ccaacgaaat cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg
     8821 cgcgacagcc tcgggctgga gtagcagtac atcattttct ttttttacag ggtgcattta
     8881 cgcctatgaa gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag
     8941 ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat
     9001 cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc
     9061 aggaagatat cgaaaccgca cagatgatgc tcgatgatcc tgaaatgcgt gagatggcgc
     9121 aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc
     9181 tgttactgcc aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgagccggaa
     9241 ccggcggcga cgaagcggcg ctgttcgcgg gcgatctgtt ccgtatgtac agccgttatg
     9301 ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag cgagggtgaa catggtggtt
     9361 ataaagagat catcgccaaa attagcggtg atggtgtgta tggtcgtctg aaatttgaat
     9421 ccggcggtca tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt
     9481 ctgcttgtac cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc
     9541 cagcagattt acgcattgat actttccgct cgtcaggggc gggtggtcag cacgttaaca
     9601 ccaccgattc ggcaattcgt attactcact tgccgaccgg gattgttgtt gaatgtcagg
     9661 acgaacgttc acaacataaa aacaaagcta aagcactttc tgtacttggt gctcgtatcc
     9721 acgctgcaga aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt aacctgctgg
     9781 ggagtggcga tcgcagcgac cgtaaccgta cttacaactt cccgcagggg cgcgttaccg
     9841 atcaccgcat caacctgacg ctctaccgtc tggatgaagt gatggaaggt aagctggata
     9901 tgctgattga accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc
     9961 aggaataatg gaatatcaat actggttacg tgaagcaata agccaacttc aggcgagcga
    10021 aagcccacgc cgtgacgctg aaatcttatt ggagtatgtc actggcaggg ggcggacttt
    10081 tattctggcc tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct
    10141 actgacacgt cgtcgcgatg gtgaacccat tgctcattta accggggtgc gagaattctg
    10201 gtcgttgccg ttatttgttt cgccagcgac cttaattccg cgcccggata cggagtgtct
    10261 ggtggagcag gcactggcgc ggttgcctga acaaccttgc cgtattctcg atctcgggac
    10321 gggtaccggg gcgattgcgc ttgcgctggc tagcgagcgc ccggactgcg aaattatcgc
    10381 tgtagatcgt atgcctgatg ctgtctccct ggcacaacgt aatgcccagc atctggcgat
    10441 caaaaatatc cacattctgc aaagcgactg gtttagcgcg ctagccgggc agcagtttgc
    10501 gatgattgtc agcaatccgc cgtatattga cgagcaggac ccacatcttc aacaaggcga
    10561 tgtccgcttt gagccgctca ctgcgctggt tgcggcagac agtggaatgg cagacatcgt
    10621 gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc ggctttctgc ttctggaaca
    10681 tggctggcag cagggcgaag cggtgcgaca agcatttatc ctcgcggggt atcatgacgt
    10741 cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg ctcggccgct attatcaatg
    10801 acaagttttt ctacactgct tagtgttcat cttattagta tcgcgctttc tgttgggcta
    10861 ttaaccttac gtttctggct acgttatcag aagcatccac aggcatttgc tcgctggacg
    10921 cgcattgtgc cgccggttgt cgatacggtg ttattgttaa gcggcattgc gttgatggct
    10981 aaagcgcaca tcctgccatt ttccgggcag gcacagtggc tgactgaaaa gctgtttgga
    11041 gttatcattt atatcgtttt gggttttatt gcactcgatt atcgtcgtat gcacagtcag
    11101 caggcgcgca ttattgcctt cccgctggcg ttggtggtgc tgtacatcat cattaaactc
    11161 gccaccacaa aagtaccgtt actggggtaa gtcatgagat cgttagctga tttcgaattt
    11221 aataaagcgc cattgtgcga aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt
    11281 ccctcgcaag atgtttacga cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc
    11341 agccagcttc tgcctttaga agagcagctg gaaaaactga tcgcgctgtt ttacggcgac
    11401 tggggattta aagcctcacg cggcgtttat cgtctttccg atgcattatg gctggaccag
    11461 gtgttaaaga atcgacaggg cagtgcggta tcattaggtg cggttttatt atgggtcgcg
    11521 aatcgtctcg atttgccgct gctgccggtg attttcccta cgcagctgat attgcgcatt
    11581 gaatgtccgg atggcgaaat ttggctgatt aatcctttta acggtgaatc gttaagcgaa
    11641 catatgctgg acgtatggtt aaagggaaat atcagcccgt cggcggaact gttttatgaa
    11701 gaccttgatg aagctgataa cattgaggta atccgcaaat tgttggatac actcaaagcc
    11761 atcgttgatg gaagaaaatc agatggagct ggcgttacgc accagcgaag ctttattaca
    11821 attcaacccg gaagatccct atgaaattcg cgatcgcggg ttgatttatg cgcaactgga
    11881 ttgcgaacac gttgcgttga acgatttaag ttatttcgtt gaacagtgtc cggaagaccc
    11941 gatcagcgaa atgatccgtg cgcaaataaa taacatcgcg cataaacata ttgtgctgca
    12001 ttaattaatc gacattttac tcaagattaa ggcgatccta tgaaacaaaa agtggttagc
    12061 attggcgaca tcaacgtagc aaatgacctg tcgttcgtac tgtttggtgg tatgaacgtg
    12121 ctggaatctc gcgatctggc gatgcgcatt tgcgagcact acgtaaccgt gacccagaaa
    12181 ctgggtatcc cttacgtgtt caaagcctct tttgacaaag ccaaccgctc ctccatccac
    12241 tcttatcgtg gaccgggcct ggaagaaggg atgaaaatct tccaggagtt gaagcagact
    12301 tttggcgtga aaattatcac cgacgttcac gaaccaagtc aggcacagcc cgttgctgat
    12361 gtcgtggatg tgattcagtt gccggcgttt cttgctcgcc agactgacct ggttgaagcc
    12421 atggcgaaaa ccggtgcggt aattaacgtc aagaaaccac agtttgtcag cccgggacag
    12481 atgggtaata tcgttgataa attcaaagaa ggcggcaacg aaaaagtgat tctttgcgat
    12541 cgcggtgcta acttcggcta tgacaacttg gttgtcgata tgctgggctt cagcattatg
    12601 aagaaagtgt ctggtaactc gccggtgatt ttcgacgtga cccacgcact gcaatgccgc
    12661 gatccgtttg gcgcagcttc cggtggtcgt cgtgctcagg tggctgagct ggcacgagcc
    12721 ggtatggcgg taggtctggc ggggctgttt attgaagcgc atccggatcc ggaacatgcg
    12781 aaatgtgatg gtccatccgc gctgccgctg gctaaactgg aaccgttcct caagcagatg
    12841 aaagcgattg atgatctggt gaaaggtttc gaagaactgg ataccagcaa gtaatctttt
    12901 ttgcttgaaa aataaagtct tagcgttctg cgttaagact tttttcatgg gtgccagata
    12961 caaaaaaggc cgcaggctgt tacccctgcg gccggtttcg ggcgcatatt gccatcacgg
    13021 cagcctgacg cccgttttca ccttacttcc ggttacgcca ccagccgaca atcgctgcgg
    13081 taataattcc cgccaggatc ggtgctgcca ggtcgtgcca gaaaatcatg gcaaa
//
LOCUS       DAZPJP010000108        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPJP010000108 DAZPJP010000000
VERSION     DAZPJP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.108"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHU9J_002760"
     CDS             <1..191
                     /locus_tag="ACHU9J_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906256.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHU9J_002761"
     CDS             191..1393
                     /locus_tag="ACHU9J_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR1906257.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHU9J_002762"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHU9J_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1906258.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWIKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHU9J_002763"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHU9J_002763"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1906259.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHU9J_002764"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHU9J_002764"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1906260.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHU9J_002765"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHU9J_002765"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1906261.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHU9J_002766"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHU9J_002766"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1906262.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHU9J_002767"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHU9J_002767"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1906263.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHU9J_002768"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHU9J_002768"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1906264.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3225 c         3576 g         3158 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 acccctttat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPJP010000109        12737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPJP010000109 DAZPJP010000000
VERSION     DAZPJP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.109"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(111..998)
                     /gene="rarD"
                     /locus_tag="ACHU9J_002769"
     CDS             complement(111..998)
                     /gene="rarD"
                     /locus_tag="ACHU9J_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1906265.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1050..1517)
                     /gene="yigI"
                     /locus_tag="ACHU9J_002770"
     CDS             complement(1050..1517)
                     /gene="yigI"
                     /locus_tag="ACHU9J_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1906266.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1682..2551
                     /gene="pldA"
                     /locus_tag="ACHU9J_002771"
     CDS             1682..2551
                     /gene="pldA"
                     /locus_tag="ACHU9J_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1906267.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2684..4513
                     /gene="recQ"
                     /locus_tag="ACHU9J_002772"
     CDS             2684..4513
                     /gene="recQ"
                     /locus_tag="ACHU9J_002772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1906268.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4577..5194
                     /gene="rhtC"
                     /locus_tag="ACHU9J_002773"
     CDS             4577..5194
                     /gene="rhtC"
                     /locus_tag="ACHU9J_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1906269.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5256..5876)
                     /gene="rhtB"
                     /locus_tag="ACHU9J_002774"
     CDS             complement(5256..5876)
                     /gene="rhtB"
                     /locus_tag="ACHU9J_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1906270.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5987..7009
                     /gene="pldB"
                     /locus_tag="ACHU9J_002775"
     CDS             5987..7009
                     /gene="pldB"
                     /locus_tag="ACHU9J_002775"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1906271.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7017..7817
                     /gene="yigL"
                     /locus_tag="ACHU9J_002776"
     CDS             7017..7817
                     /gene="yigL"
                     /locus_tag="ACHU9J_002776"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1906272.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPYYLRKLYLS"
     gene            7893..8792
                     /gene="bioP"
                     /locus_tag="ACHU9J_002777"
     CDS             7893..8792
                     /gene="bioP"
                     /locus_tag="ACHU9J_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1906273.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8680..9633)
                     /gene="metR"
                     /locus_tag="ACHU9J_002778"
     CDS             complement(8680..9633)
                     /gene="metR"
                     /locus_tag="ACHU9J_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1906274.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9869..12130
                     /gene="metE"
                     /locus_tag="ACHU9J_002779"
     CDS             9869..12130
                     /gene="metE"
                     /locus_tag="ACHU9J_002779"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1906275.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12252..12374)
                     /locus_tag="ACHU9J_002780"
     CDS             complement(12252..12374)
                     /locus_tag="ACHU9J_002780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906276.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12415..12660)
                     /locus_tag="ACHU9J_002781"
     CDS             complement(12415..12660)
                     /locus_tag="ACHU9J_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1906277.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         2995 a         3219 c         3410 g         3113 t
ORIGIN      
        1 gggggtattt cctgtaacaa atattgttct ttttcttatc ttaatttttt tcaaaaaaag
       61 ataaataagt cagtgggtag gctctttaaa cggcctaccc atgttaattc ttaacttttt
      121 ctacgctgcg tataaatcgc atccatcaca aaaattgcca gcgccaccca aataaaggcg
      181 aaagtcacca tcttatcggc acccggtttt tcaccataaa acgtcacagc cagcaggaac
      241 atcagcgtcg ggccaatgta ctggaaaaag cctaacgttg agagacgcaa gcgcgtggca
      301 gcggcggtaa aacacaacag cggtacggta gtgacaatac cggcggcgat cagcagtaaa
      361 ttcagcgaca tcgggttttg ccccatatgg ctggttgagc tgtcggcaat agcaaacagg
      421 taaattgccg ccacgggcag cagccacatg gtttcgatta acatgccggt ttgcgcttca
      481 acggcaatct tcttgcgtac cagaccgtag aaggcaaaac taaatgccag tcccagcgcg
      541 ataataggta gcgaaccaaa agtccacagc tggactaaca cgccacatat cgccagaatc
      601 accgccagcc attgcatccg gcggaatcgc tcgccgagga aaatcatccc cagcacaatg
      661 ttcaccagcg ggttaataaa gtaaccaagg ctcgcttcca gcatatggtg attgttcacc
      721 gcccagataa acagtagcca gttgccacca atcagcacgg cagagactgc cagcataaaa
      781 attttctgtg gcgtctgaat cagcgttttt aaataggacc actggcggca aatgctcatc
      841 agcaccacca taaagaaaaa cgaccagatc acgcgatgcg tcaggatttc atcggcgggc
      901 acgtagtaaa tcaacttgaa gtacgctggc gctatacccc aaataaaata agcggcaaga
      961 gcgagtaata cgccctgccg cgtttgtttt gcatccatcg ggaatactca tttttaattg
     1021 gtaacagcag tttacctgct tttatgtctt caacctacca tataggtggc ggtggcactg
     1081 gcaatataaa gctgttcttc attgtgtaat tcaacgcggg cgacggcgac tttattgcct
     1141 gcacgcaaca ggctactggt agcagtaaaa cgctcgcccc tgcctgggcg cagataatca
     1201 acgcgaagat caatggtccc catccgcgat agccgctggc gtagttcatc ttcactgatg
     1261 gtttcgtggc gggttaaggt acttcccacg cacaccagac cggcggcgac atccagcgcc
     1321 gacgcaatga ccccgccgtg caaaatgctt tgcgcccagt tgcccaccat cattggctga
     1381 tttttaaagg ccagctgtgc gaactctttt tcgtaacgct ccagttccat ccccaatgcg
     1441 cggttaaatg gcatgtgata aacaaacatc tcacccacta atttcagggc ttgttcagcg
     1501 gtcagtacgg cagacatatc atccttacac ttcattggtt aatgaaatgt tgattttatg
     1561 cttctttgtt gttggtttct actttaggaa gggataacta acggctatgg agttaagtat
     1621 gtaaaatagc ccgcagaaaa atattcacct tatcaataat tcgttacgga gaacacgacc
     1681 gatgcggact ctgcagggct ggttgttgcc ggtgtttatg ttgcctatgg cagtatatgc
     1741 acaagaggca acggtgaaag aggtgcatga cgcgccagcg gtgcgtggca gtattatcgc
     1801 caatatgctg caggagcatg acaatccgtt cacgctctat ccttatgaca ccaactacct
     1861 catttacacc caaaccagcg atctgaataa agaagcgatt gccagttacg actgggcgga
     1921 aaatgcgcgt aaggatgaag taaagtttca gttgagcctg gcatttccgc tgtggcgtgg
     1981 gattttaggc ccgaactcgg tgttgggtgc gtcttatacg caaaaatcct ggtggcaact
     2041 gtccaatagc gaagagtctt caccgtttcg tgaaaccaac tacgaaccgc aattgttcct
     2101 cggttttgcc accgattact gttttgcagg ttggacgctg cgcgatgtgg agatggggta
     2161 taaccacgac tctaacgggc gttccgaccc gacctcccgc agctggaacc gcctttatac
     2221 tcgcctgatg gcagaaaacg gtaactggct ggtagaagtg aagccgtggt atgtggtggg
     2281 taatactgac gataacccgg atatcaccaa atatatgggt tactaccagc ttaaaatcgg
     2341 ctatcacctc ggtgatgcgg tgttgagtgc gaaaggacag tacaactgga ataccggtta
     2401 cggcggcgcg gagttaggct taagttaccc gatcaccaaa catgtgcgcc tttatactca
     2461 ggtttacagc ggctatggcg aatcgctcat cgactataac ttcaaccaga cccgtgtcgg
     2521 tgtaggagtt atgctaaacg acttgttttg atgaacggtt gagtggttgg caaatctgga
     2581 acccagcatc caggattacc ctctcagaga ctaaaagcat tgcagtttct cgcgcaggcg
     2641 ctgaaaatag cgcctgtttt tatttcaggc aatcggggtg aatgtggcgc aggcggaagt
     2701 gttgaatctg gagtccggag ctaaacaggt tttacaagaa acctttggct accaacagtt
     2761 tcgccccggc caggaagaaa ttatcgacac tgtgctttcc ggtcgcgatt gcctggtcgt
     2821 catgcccacc ggtggcggaa aatccctttg ctatcaaatt cctgccttat tgctaaacgg
     2881 ccttaccgtg gttgtttcac cgctgatttc gttgatgaaa gatcaggtgg atcaactgca
     2941 agccaacggc gtggcggcgg cgtgccttaa ctcgacgcaa acccgcgagc agcaacttga
     3001 agtgatgaca ggctgccgca ccgggcaaat tcgtctgctt tatatcgccc cggaacgcct
     3061 gatgctggat aactttcttg agcatctggc gcactggaat ccggtgttat tagccgttga
     3121 tgaagcgcac tgtatctccc aatggggcca cgatttccgc ccggaatatg ccgcgcttgg
     3181 tcagttacgc cagcgtttcc cgaccctgcc gtttatggcg ctgactgcca cagccgacga
     3241 caccacgcgc caggatatcg tgcgcttgct ggggctgaac gatccgctga ttcaaatcag
     3301 cagttttgac cgtccgaata ttcgctacat gctgatggag aaattcaaac cgctcgatca
     3361 gttgatgcgc tacgtgcagg aacagcgcgg taagtccggc attatctact gcaacagccg
     3421 ggcgaaagta gaagacaccg ctgcgcgcct gcaaagcaag ggtattagcg cggcggccta
     3481 tcatgccggg ctggaaaata atgtccgcgc cgacgtgcag gagaaattcc agcgcgatga
     3541 cctgcaaatt gtggtggcga cggtggcgtt cggcatgggc atcaataaac caaacgttcg
     3601 cttcgtggtc cactttgata ttccgcgcaa tatcgaatcc tattatcagg aaaccggtcg
     3661 cgccgggcgt gatggcctgc ctgcggaagc gatgctgttt tacgatccgg ctgatatggc
     3721 gtggctgcgc cgttgtctgg aagagaagcc gcaggggcag ttgcaggata ttgagcgcca
     3781 caaactcaat gcgatgggcg cgtttgccga agcgcaaact tgccgtcgtc tggtattgct
     3841 gaactatttt ggcgaagggc gtcaggagcc gtgcgggaac tgcgatatct gcctcgatcc
     3901 gccgaaacag tacgacggtt caaccgatgc tcagattgcc ctttccacca ttggtcgtgt
     3961 gaatcagcgg tttgggatgg gttatgtggt ggaagtgatt cgtggtgcta ataaccagcg
     4021 tatccgcgac tatggtcatg acaaactgaa agtctatggc atgggccgtg ataaaagcca
     4081 tgaacattgg gtgagcgtga tccgccagct gattcacctc ggcctggtga cgcaaaatat
     4141 tgcccagcat tctgccctac aactgacaga ggccgcgcgc ccggtgctgc gcggcgaatc
     4201 ctctttgcaa cttgccgtgc cgcgtatcgt ggcgctcaaa ccgaaagcga tgcagaaatc
     4261 gttcggcggc aactatgatc gcaaactgtt cgccaaatta cgcaaactgc gtaaatcgat
     4321 tgccgatgaa agtaatgtcc cgccgtacgt ggtgtttaac gacgcaacct tgattgagat
     4381 ggctgaacag atgccgatca ccgccagcga aatgctcagc gttaacggcg ttgggatgcg
     4441 caagctggaa cgctttggta aaccgtttat ggcgctgatt cgtgcgcatg tcgatggcga
     4501 cgacgaagag tagtcagcag cataaaaaag tgccaggatg aagactccgt aaacgtttcc
     4561 cccgcgagtc aaatgtatgt tgatgttatt tctcaccgtc gccatggtgc acattgtggc
     4621 gcttatgagc cccggtcccg atttcttttt tgtctctcag accgctgtca gtcgttcccg
     4681 taaagaagcg atgatgggcg tgctgggcat tacctgcggt gtaatggttt gggctgggat
     4741 tgcgttgctt ggcctgcatt tgattatcga aaaaatggcc tggctgcata cgcttatggt
     4801 tggcggtggc ctgtatctct gctggatggg ttaccagatg ctgcgcggcg cgctgaaaaa
     4861 agaggcggtt tctgcacctg cgccacaggt cgagctggcg aaaagtgggc gcagtttcct
     4921 gaaaggttta ctgaccaatc tcgctaatcc gaaagcgatt atctactttg gctcggtgtt
     4981 ctcattgttt gtcggtgata acgttggcac taccgcgcgc tggggcattt ttgcgctgat
     5041 cattgtcgaa acgctggcgt ggtttatcgt cgttgccagc ctgtttgccc tgccgcaaat
     5101 gcgccgtggt tatcaacgtc tggcgaagtg gattgatggt tttgccgggg cgttatttgc
     5161 cggatttggc attcatttga ttatttcgcg gtgatgccag acgcgtcttc agagtaagtc
     5221 ggataaggcg tttacgccgc atccgacatt atttttcacg catgcctcgc cgatgctaac
     5281 agcgctccca ccagcataaa caacgagccg aaaatcttat tcagcgcctt catctgcttt
     5341 ggtcctttaa tccatagagc aatccgttga gcaagggtgg cgtaaccgat catcacaata
     5401 atatcgacca caatagtggt gacgccgagc acgatatact gcatcagttg cggctgttgc
     5461 ggcatgatga attgcggaaa tagcgccgcc agaaacacaa tacttttggg attggtgaga
     5521 ttcacaaaaa ctgcgcgctg gaacaaatgt cgacgcgatt gagtagaggc cagcgattta
     5581 aggtcaattg caccagcggc gcgccactgc tggattccca gccaaatcaa gtaagccgcg
     5641 cctgcccact tcaacacttc aaacgcaatc actgagcggg aaaatagcgt ccccaacccc
     5701 acgccaacca gcacaatatg aatcgccagt ccggtctgaa gcccagcaat agacgccacc
     5761 gcgccgcgat aaccgtggtt gagcgaggtg gtcatagtgt tgattgcacc agagcctggc
     5821 gacaggctta aaatgatcga tgtcagcagg taggcaaacc accattctaa ggtcatgatg
     5881 aactcccggt gtgtctattt ttgtgccaca atacgctact gtcgcagcgt tgtgtcaggc
     5941 acgctaaaaa aaacgatttt acgtggttta agaggcagat tacccgatgt ttcagcagca
     6001 aaaagactgg gaaacaagag aaaacgcgtt tgctgctttt accatgggac cgctgactga
     6061 tttctggcgt cagcgtgatg aagcagagtt tactggtgtg gatgacattc cggtgcgctt
     6121 tgtccgtttt cgcgcacagc accatgaccg ggtggtagtc atctgcccgg ggcgtattga
     6181 gagctacgta aaatatgcgg aactggccta tgacctgttc catttggggt ttgatgtctt
     6241 aatcatcgac catcgcgggc agggacgttc cggtcgcctg ttagccgatc cgcatctcgg
     6301 gcatgttaat cgctttaatg attatgttga tgatctggcg gcattctggc agcaggaggt
     6361 tcagcccggt ccgtggcgta aacgctatat actggcacat tcgatgggcg gtgcgatctc
     6421 cacattattt ctgcaacgcc atccaggtgt atgtgacgcc attgcgctaa ctgcgccaat
     6481 gtttgggatc gtgattcgta tgccgtcatt tatggcacgg cagatcctca actgggccga
     6541 agcgcatcca cgtttccgtg atggctatgc aataggcacc gggcgctggc gcgcgttgcc
     6601 gtttgctatc aacgtactga cccacagcag acagcgatat cgacgtaact tacgcttcta
     6661 tgctgatgac ccaacgattc gcgtcggtgg gccgacctac cattgggtac gcgaaagtat
     6721 tctggctggc gaacaggtgt tagccggtgc gggtgatgac gccacgccaa cgcttctctt
     6781 gcaggctgaa gaggaacgcg tggtggataa ccgcatgcat gaccgttttt gtgaactccg
     6841 caccgccgcg ggccatcctg tcgaaggagg acgaccgttg gtaattaaag gtgcttacca
     6901 tgagatcctt tttgaaaagg acgcaatgcg ctcagtcgcg ctccacgcca ttgttgattt
     6961 tttcaacagg cataactcac ccagcggaaa ccgctctaca gaggtttaaa tttcttatgt
     7021 accaggttgt tgcgtctgat ttagatggca cgttactttc tcccgaccat acgttatccc
     7081 cttacgccaa agaaaccctg aagctgctca ccgcgcgcgg catcaacttt gtgtttgcga
     7141 ccggtcgtca ccacgttgat gtggggcaaa ttcgcgataa tctggagatc aagtcgtaca
     7201 tgatcacctc caacggcgcg cgcgttcacg atctggatgg caatctgatt tttgctcata
     7261 acctggatcg cgacattgcc agcgatctgt ttggcgtagt caacgacaat ctggacatca
     7321 ttactaacgt ttatcgcgac gacgaatggt ttatgaatcg ccatcgcccg gaagagatgc
     7381 gcttttttaa agaagcggtg ttcaaatatg cgctgtatga gcctggatta ctggagccgg
     7441 aaggcgtcag caaagtgttc ttcacctgcg attcccatga acaactgctg ccgctggagc
     7501 aggcgattaa cgctcgttgg ggcgatcgcg tcaacgttag tttctctacc ttaacctgtc
     7561 tggaagtgat ggcgggcggc gtttcaaaag gccatgcgct ggaagcggtg gcgaagaaac
     7621 tgggctacag cctgaaggat tgtattgcgt ttggtgacgg gatgaacgat gccgaaatgc
     7681 tgtcgatggc ggggaaaggc tgcattatgg gcagtgcgca ccagcgtctg aaagaccttc
     7741 atcccgagct ggaagtgatt ggtactaatg ccgacgacgc ggtgccgtat tatctgcgta
     7801 aactctattt atcgtaatcg ttctttattt ggtcagttgt caacctgata cttcgctaca
     7861 atggatactc gttaatcaaa gagttttcca ttgtggcgct acttatcatc accacgattc
     7921 tgtgggcctt ctcctttagc ttttatggcg agtaccttgc ggggcacgtc gatagctatt
     7981 ttgcggtgct ggtgcgcgtt ggcctggcgg cactcgtttt tctgccgttt ctgcgtaccc
     8041 gtggcaatag cctgaaaacg gtcggcctgt atatgctggt gggcgcgatg cagctcggcg
     8101 tgatgtatat gctgagtttc cgcgcttatc tctacctgac ggtctccgaa ttgttgctgt
     8161 tcaccgtact gacgccgctc tacatcacgc tgatttatga catcatgagt aagcgccgtc
     8221 tgcgctgggg ctatgctttt agcgccttgc tggcggtgat tggtgccggg attattcgct
     8281 atgatcaggt taccgaccat ttctggactg gcttgttgct ggtgcaactc tccaatatca
     8341 cttttgccat cggcatggtg ggttacaaac gcctgatgga aactcgcccg atgccgcagc
     8401 ataacgcctt tgcgtggttc tatcttggcg cgttcctggt ggcggtaatt gcatggttct
     8461 tgctgggaaa tgcgcagaaa atgccgcaaa ccacgttgca atggggcatt ctggtgtttc
     8521 ttggcgtggt ggcttccggg attggctact ttatgtggaa ctacggcgcg acgcaggtgg
     8581 acgccggaac gctgggcatt atgaataata tgcacgttcc ggcagggctg ctggtaaacc
     8641 tggctatctg gcaccaacag ccgcactggc caacgttcat tacaggcgcg ctggtgatcc
     8701 tggcctcact gtgggtgcat cgtaagtggg tcgctccgcg ctcttcacaa acggcagatg
     8761 atcgcaggcg tgattgcgcg ctgagcgaat aaatgcttct gtaatcggct gacgctgctc
     8821 gccatcgcgc acggcggcgt acagtcggct ccacaagcct tcgcccaggg ttttggtcac
     8881 caccagaccc tggcgctcaa aactctctac tacccaatgc ggtagcgcgg caatacccat
     8941 ccgcgcggca accatctgaa tcaacaataa ggtgttatcg acgcttttca gtgacgggct
     9001 gacgcctgcc ggctgaagaa aatgccgcca gacatccagt cgactacgct gcaccggata
     9061 aatcaacagc gtctcgctgg cgagatcttc cggtgtaatt cgcgttttcg ccgccagtgg
     9121 atggtcaggt gctaacacca gacgcacttc atagtcgaac atcggcgaat aatgcaggcc
     9181 actgcgcggc aggatatcgg aggtcatcac cagatccagc tcgccctgtt gcaacgcagg
     9241 ctgcgggtca aacgtcacgc ccgatttaaa atccatctct acctgcggcc agttcttatg
     9301 gaaattttct aacgcgggcg tcagccactg aatacagcta tggcactcaa tggcaatgcg
     9361 cagacgcgtc tgctgcggtt cattgcaggc ttgcagtgcc tggctgattt gcggcagcac
     9421 ctggtttgcc agttgcaaca ggatttctcc ctgcggtgta aagcgtagcg gctggctctt
     9481 acgcacaaat agccggaagc caaggcgttg ttccagatcg ctaaactggt gagacagggc
     9541 ggattgcgtc tgatgcaacg tcgccgcagc ggctgcgaga gagccgcagt tccgcaacgc
     9601 ttgtagcgtt ttcaggtgtt ttacttcgat catgaaagtc cttcacttcg gcatgaataa
     9661 tttgcgcttg aggaatatac agtaaccgcc aattatggat gtgtaaacat ctggacggct
     9721 aaaatccttc gtcttttaaa tttatggtgc gttggctgcg tttctccacc ccggtcactt
     9781 acttcagtaa gctcccgggg atgaataaac ttgccgcctt ccctaaattc aaaatccata
     9841 ggatttacat ataattagag gaagaaaaat gacaatattg aatcacaccc tcggtttccc
     9901 tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg cggggaactc
     9961 cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt gctcgtcact gggatcaaca
    10021 aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt gcctggtacg atcatgtact
    10081 gaccaccagt ttgctgctgg gtaatgttcc gccacgtcat cagaacaaag acggttcggt
    10141 agatatcgac accctgttcc gtattggtcg tggacgtgcg ccgactggcg aacctgcggc
    10201 ggcagcggaa atgaccaaat ggtttaacac caactatcac tacatggtgc cggagttcgt
    10261 taaaggccaa cagttcaaac tgacctggac gcagctgctg gaggaagtgg acgaggcgct
    10321 ggcgctgggc cacaacgtta agcctgtgct gctggggccg gttacttacc tgtggctggg
    10381 gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg aacgacattc tgccggttta
    10441 tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc
    10501 cgcgctggta ctggaactac cacaggcgtg gctggacgca tacaaacccg cttacgacgc
    10561 gctccaggga caggtgaaac tgctgctgac cacctatttt gaaggcgtaa cgccaaatct
    10621 cgacacgatt actgcgctgc ctgttcaggg tctgcatgtt gatctcgtac atggtaaaga
    10681 tgacgttgct gaactgcaca agcgcctgcc ttctgactgg ttgctgtctg cgggtctgat
    10741 caatggtcgt aacgtctggc gcgccgatct taccgagaaa tatgcgcaaa ttaaggacat
    10801 tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca gccccatcga
    10861 cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct tcgccctaca
    10921 aaaatgccat gaactagcac tgctgcgcga tgcgctgaac agtggtgaca cggcagctct
    10981 ggcagagtgg agcgccccaa ttcaggcgcg tcgtcactct actcgtgtac ataatccggc
    11041 ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga atgtctatga
    11101 agtgcgtgct gaagcccagc gtgcacgttt taaactgcca gcgtggccga ccaccacgat
    11161 tggttccttc ccgcaaacca cggaaattcg taccctgcgt ctggatttca aaaagggcaa
    11221 tctcgacgcc aataactacc gcacgggcat tgcggaacat atcaggcagg ccattgttga
    11281 acaggaacgt ttgggactgg atgtgctggt acatggcgag gccgagcgta atgacatggt
    11341 ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg caaaacggtt gggtacagag
    11401 ctacggttcc cgctgcgtga agccaccgat tgttattggt gacgttagtc gcccggcacc
    11461 gattaccgtg gagtgggcga agtatgcgca atctctgact gataaaccgg tgaaagggat
    11521 gctgactggc ccggtgacta ttctctgctg gtcgttcccg cgtgaagatg tcagccgtga
    11581 aaccatcgcc aaacagattg cgctggcgct gcgtgatgaa gtggccgatc tggaagccgc
    11641 tggaattggc atcatccaga ttgacgaacc ggctctgcgc gaaggtttac cgctgcgtcg
    11701 tagcgactgg gatgcgtatc tccagtgggg cgtagaggcc ttccgtatca acgccgccgt
    11761 ggcgaaagat gacacacaaa tccacactca catgtgttat tgcgagttca acgacatcat
    11821 ggattcgatt gcggcactgg acgcagacgt catcaccatc gaaacctcgc gttctgacat
    11881 ggagttgctg gagtcgttcg aagagtttga ttatccgaat gaaatcggtc caggcgtcta
    11941 tgacattcac tcgccaaacg taccgagcgt ggaatggatt gaagccttgc tgaagaaagc
    12001 ggcaaaacgc attccggcag agcgcctgtg ggttaacccg gactgtggcc tgaaaacgcg
    12061 cggctggcca gaaacccgcg cggcactggc gaacatggtg caggcggcgc agaatttgcg
    12121 tcggggatga tgaatattaa cgagtcactg taaattgcag tacgatgaaa gaggcaaaac
    12181 tgcctgatac taggcctaca taatttatgc aatttattga atttttgggt tttgttggcc
    12241 ggacaaggcg ttcacgccgc atccggcatg aacaacgcgc actttgccaa taatctgatg
    12301 gcttccatca ccggaagcca ttttgctatc tgcgcccgtt cagatacaga aataaccgaa
    12361 tcatttttac gcaaaatata atcctgagcg atttgttcag taatcaacgc tacgttattt
    12421 attctcactt ttcacgaaat tgtatccctt ttcagtaacg tggtagtgag gcatagactt
    12481 atgtccgcta tcctcaataa taaccaaacc ctcgttagct aatttgttaa caatgttaac
    12541 aacattaccg aatcctggaa tttgccttat ggatagtgtg cgatcgagat tcatccagtt
    12601 ccagtttata tctttctcag ccataatttt cagtacgtta atttcagttt cagttatcat
    12661 agtcaagtgc atcctggtgg tataagtcat taagttctcc cttaagttta taatcttcta
    12721 gagtagcagt atgcgta
//
LOCUS       DAZPJP010000110        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPJP010000110 DAZPJP010000000
VERSION     DAZPJP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.110"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHU9J_002782"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHU9J_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1906278.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHU9J_002783"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHU9J_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1906279.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHU9J_002784"
     CDS             1797..1970
                     /locus_tag="ACHU9J_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906280.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHU9J_002785"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHU9J_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1906281.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHU9J_002786"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHU9J_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1906282.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHU9J_002787"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHU9J_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1906283.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHU9J_002788"
     CDS             complement(4268..5191)
                     /locus_tag="ACHU9J_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1906284.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHU9J_002789"
     CDS             5408..6709
                     /locus_tag="ACHU9J_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1906285.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHU9J_002790"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHU9J_002790"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1906286.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHU9J_002791"
     CDS             8771..8995
                     /locus_tag="ACHU9J_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1906287.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHU9J_002792"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHU9J_002792"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1906288.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHU9J_002793"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHU9J_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1906289.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHU9J_002794"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHU9J_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1906290.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHU9J_002795"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHU9J_002795"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1906291.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZPJP010000111        12291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPJP010000111 DAZPJP010000000
VERSION     DAZPJP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.111"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..253)
                     /gene="ydjO"
                     /locus_tag="ACHU9J_002796"
     CDS             complement(<1..253)
                     /gene="ydjO"
                     /locus_tag="ACHU9J_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR1906292.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDT"
     gene            complement(530..793)
                     /gene="cedA"
                     /locus_tag="ACHU9J_002797"
     CDS             complement(530..793)
                     /gene="cedA"
                     /locus_tag="ACHU9J_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1906293.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            976..3237
                     /gene="katE"
                     /locus_tag="ACHU9J_002798"
     CDS             976..3237
                     /gene="katE"
                     /locus_tag="ACHU9J_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1906294.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3495..4253)
                     /gene="chbG"
                     /locus_tag="ACHU9J_002799"
     CDS             complement(3495..4253)
                     /gene="chbG"
                     /locus_tag="ACHU9J_002799"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1906295.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4266..5618)
                     /gene="celF"
                     /locus_tag="ACHU9J_002800"
                     /gene_synonym="chbF"
     CDS             complement(4266..5618)
                     /gene="celF"
                     /locus_tag="ACHU9J_002800"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1906296.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5723..6565)
                     /gene="chbR"
                     /locus_tag="ACHU9J_002801"
     CDS             complement(5723..6565)
                     /gene="chbR"
                     /locus_tag="ACHU9J_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR1906297.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6573..6923)
                     /gene="chbA"
                     /locus_tag="ACHU9J_002802"
     CDS             complement(6573..6923)
                     /gene="chbA"
                     /locus_tag="ACHU9J_002802"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1906298.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6974..8332)
                     /gene="chbC"
                     /locus_tag="ACHU9J_002803"
     CDS             complement(6974..8332)
                     /gene="chbC"
                     /locus_tag="ACHU9J_002803"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR1906299.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(8417..8737)
                     /gene="chbB"
                     /locus_tag="ACHU9J_002804"
     CDS             complement(8417..8737)
                     /gene="chbB"
                     /locus_tag="ACHU9J_002804"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1906300.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(9036..9374)
                     /gene="osmE"
                     /locus_tag="ACHU9J_002805"
     CDS             complement(9036..9374)
                     /gene="osmE"
                     /locus_tag="ACHU9J_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1906301.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9576..10403
                     /gene="nadE"
                     /locus_tag="ACHU9J_002806"
     CDS             9576..10403
                     /gene="nadE"
                     /locus_tag="ACHU9J_002806"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1906302.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10633..11520
                     /gene="cho"
                     /locus_tag="ACHU9J_002807"
     CDS             10633..11520
                     /gene="cho"
                     /locus_tag="ACHU9J_002807"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1906303.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11480..12055)
                     /gene="ves"
                     /locus_tag="ACHU9J_002808"
     CDS             complement(11480..12055)
                     /gene="ves"
                     /locus_tag="ACHU9J_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1906304.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3293 a         3125 c         2774 g         3099 t
ORIGIN      
        1 acgtatcaaa actaagtgct ggagctcctg gctttgaggc atcatagtta atgttagaca
       61 tataggcaat catacgattg aagttcgact ctgctatttc atcgcagtca cattgacccg
      121 ttgatataaa gaaagtgagt gccgccatgg tagactcaag cctttcttta aaagagtcga
      181 aagccaggct tttaatattt aaatcaccat aattactctg tattaagttt gtagaaaaca
      241 tctcccgccc catattgtta acaaaattat tatctcattt aaatctaagt cacttacaat
      301 ataagtttaa gagcgacgcc acaggatgaa ctatcaaaaa tagctcatca tgattagcaa
      361 aacttaacca ttttaaaata aataaacaat taaagaaaaa agatcactta tttatagcaa
      421 tagctcgtca aaggctgctt tttgttacag gtggtttgaa tgaatgtagc aacgaaatgc
      481 agaatttcag gtcatgtaac tcccggcaaa accgggaagt atgtaatcct tactcagtca
      541 cttccccttc ctggcggatc tgatttgccc aacgttgggc agattcaggc acagtaaacg
      601 ccggtgagcg cagaaatgac tctcccatca gtacaaacgc aacatatttg ccacgcagca
      661 tccagacatc acgaaacgaa tccatcttta tcgcatgttc tggcggcgcg ggttccgtgc
      721 gtgggacata gctaataatc tggcggtttt gctggcggag cggtttcttc attactggct
      781 tcactaaacg catattaaaa attagaaaaa ctgtagttta gccgatttag cccctgtacg
      841 tcccgctttg cgtgtatttc ataacaccgt ttccagaata gtctccgaag cgggatctgg
      901 ctggtggtct atagttagag agttttttga ccaaaacagc ggccctttca gtaataaatt
      961 aaggagacga gttcaatgtc gcaacataac gaaaagaacc tacatcagca ccagtcacca
     1021 ctacacgatt ccagcgaagc gaaaccgggg atggactcac tggcacctga ggacggatct
     1081 catcgtccag cggctgaacc aacaccgcca ggtgcacaac ctaccgcccc tgggagcctg
     1141 aaagcccctg atacacgtaa cgaaaaactt aattctctgg aagacgtacg caaaggcagt
     1201 gaaaattatg cgctgaccac taatcagggc gtgcgcatcg ccgatgatca aaactcactg
     1261 cgtgccggta accgtggtcc aacgctgctg gaagatttta ttctgcgcga gaaaatcacc
     1321 cactttgacc atgagcgcat cccggaacgt attgttcatg cacgcggatc agcagctcac
     1381 ggttatttcc agccatataa aagcttaagc gatatcacca aagcggattt cctctcagat
     1441 ccgaacaaaa tcaccccagt atttgttagg ttctctaccg ttcagggtgg cgctggctct
     1501 gcagataccg tgcgtgatat ccgtggtttt gccaccaagt tctatactga agagggtatt
     1561 tttgacctcg ttggcaataa cacgccaatc ttctttatcc aggatgcgca taaattcccc
     1621 gattttgttc atgcggtaaa accagaaccg cactgggcaa ttccacaagg gcaaagtgcc
     1681 cacgatactt tctgggatta tgtttctctg caacctgaaa ctctgcacaa cgtgatgtgg
     1741 gcgatgtcgg atcgcggcat cccccgcagt taccgcacca tggaaggctt cggtattcac
     1801 accttccgcc tgattaatgc cgaagggaag gcaacgtttg tacgtttcca ctggaaacca
     1861 ctggcaggta aagcctcact cgtttgggat gaagcacaaa aactcaccgg acgtgacccg
     1921 gacttccacc gccgcgagtt gtgggaagcc attgaagcag gcgattttcc ggaatacgaa
     1981 ctgggcttcc agttgattcc tgaagaagat gaattcaagt tcgacttcga tcttctcgat
     2041 ccaaccaaac tgatcccgga agaactggtg cccgttcagc gtgtcggcaa aatggtgctc
     2101 aatcgcaatc cggataactt ctttgccgaa aacgaacaag cagcattcca tccaggtcat
     2161 attgttcctg gtctggactt caccaacgat ccgctgttgc agggacgttt gttctcctat
     2221 accgatacac aaatcagtcg tcttggtggg ccgaatttcc atgagattcc gattaaccgt
     2281 ccgacctgcc cttaccacaa cttccagcgt gatggcatgc atcgtatggg gatcgacact
     2341 aacccggcga attacgaacc gaactcgatc aacgataact ggccgcgcga aacaccgccg
     2401 ggaccgaaac gcggcggttt tgaatcatat caggaacgcg tggaaggaaa taaagttcgt
     2461 gagcgcagtc catcgtttgg cgaatattat tcccatccgc gtctgttctg gctaagtcag
     2521 acgccattcg agcagcgcca tattgtcgat ggtttcagtt ttgagttaag caaagtcgtt
     2581 cgtccgtata ttcgtgagcg cgttgttgac cagctggcgc atattgatct cactctggcc
     2641 caggcggtgg cgaaaaatct cggtatcgaa ctgactgacg accagctgaa tatcacccca
     2701 cctccggacg tcaacggtct gaaaaaggat ccatccttaa gtttgtacgc cattcctgac
     2761 ggtgatgtga aaggtcgcgt ggtagcgatt ttacttaatg atgaagtgag atcggcagac
     2821 cttctggcca ttctcaaggc cctgaaggcc aaaggcgttc atgccaaact gctctactcc
     2881 cgaatgggtg aagtgactgc ggatgacggt acggtgttgc ctatagccgc tacctttgcc
     2941 ggtgcacctt cgctgacggt cgatgcggtc attgtccctt gcggcaatat cgcggatatc
     3001 gctgacaacg gcgatgccaa ctactacctg atggaagcct acaaacacct taaaccgatt
     3061 gcgctggcgg gagacgcgcg caagtttaaa gcaacaatca aggtcgctga ccagggtgaa
     3121 gaagggattg tggaagctga cagcgctgac ggtagtttta tggatgaact gctaacgctg
     3181 atggcagcac accgcgtgtg gtcacgcatt cctaagattg acaaaattcc tgcctgatgg
     3241 gaggcggcgc aattgcgccg cctcaaatga tttacatagt gcgctttgtt tatgccggat
     3301 gcggcgtgaa cgccttatcc ggcctacaaa actgtgcaaa ttcaatatat tgcaggaaac
     3361 acgtaggcct gataagcgaa gcgcatcagg cagttttgcg tttgtcagca gtctcaagcg
     3421 gcgcagttac gccgcctttg taggaattaa tcgccggatg caaggttcac gccgcatctg
     3481 gcaaacatcc tcacttacac atcccgataa ctccccaacc gataaccacg ctgagcgata
     3541 gcacctttca acgacgctga tgtcaacaca tccagctccg ttaagcgtgg gaaacagtaa
     3601 gcactctgac ggatagtatt atcgataaac gccggatgac acatcacctc cagcgaacga
     3661 tcaccccgat ggcttgcgtc atccagcact tgcaggaaca gtgcctcgct aatctcttca
     3721 ccataaaacg cgctactgaa tccctgagaa ctgcgcagat tagccggtaa atcaccagag
     3781 ttagatagcg gctgacggtc gatgcgcaac gcaatcccct cttcagccgc aaacctggca
     3841 acaatcggga aaatctgcgg gaacatatgc acatgatgat ggctatcaag atgcgtaggt
     3901 ttgcgtccaa atagctcaat gaaacgcaaa tactgactgg caagctcctg agtaatttct
     3961 tccagcggta aagcatcttc ttctgccaac tgccagatcc attttcccag cacaccatcg
     4021 cgggttaacc ccggcatagc tgtcagtggc ttgcccatag taaggacaaa gtacatccct
     4081 atggccagac tcggttcatc acgactcaac tgcaccgcat ggtcaatagc ctgcccattc
     4141 accagcgccg tcgtcgatgt gacaatccca ttgcgacagg cctcgataat gccgtagttc
     4201 tgacctttgc ttaagccaaa atcatcggca ttaacaatca gtaagcgttc cataatcagc
     4261 ctcggttaat gtgctttttt aagctctgcg atgcagtcgg caaaatttgg cagccatttc
     4321 tcgtgcgcca gaatcatctc gcgtgccagc agctcagcat cgcgatcgga atgcaccaac
     4381 ggactaaggt ttagcgccag taacacatcg ttaaattctc cgctaagtgc ggcgttgctg
     4441 gcagcaatct cgaagccttt aatggtgtga atcagcccca tcactttatc atcgaaatgc
     4501 gtaatgcgcg gatgtggcgt cgcgccatcg cgccccagct tacaggtcat ttctaccgcc
     4561 cagtctgccg gaatattatc aatatgccca tgatgcggga tattaacgta atgttcagct
     4621 tgcttgtcgt tgtagatagc gttgatcact tcgcacgctg catcagagta ataagcccca
     4681 ccgcgctgtt ccagttcttt cggcttaact ttcaactccg ggtttttata cagctcaaaa
     4741 agttgtttct cgactttctg tactacctgc gctcgtgcgc cgcctttgta gtattcgccc
     4801 atttcaatag ccagcatctc tttttgcttg aagtaataaa gcaaataaga acacggcaac
     4861 agattcagag aacgaattaa gccttcgcta aatggcagat cgaaaatatt tttaacgcca
     4921 gatgctttta actgcccgga tgccacacca tcaagcaatt cggcaaagcg cgacttgcca
     4981 tttaccagca catccttaat gaacaccata tggttgaggc cgaacaaatc gatagataaa
     5041 tcatcgctgt ctttcagcat cagaacatcg tgaataaaca tcttcatgcc gatcggaata
     5101 ttacacacgc cgataaagcg tttaaatccg gtatgacgat aaacggcttc agtaaccatt
     5161 cccgccgggt tagtgaagtt aatcacccat gcattcggac aaagttcttc gacatctttt
     5221 acgatgtcaa aaatcaccgg aatggtacgc agacctttaa acagaccgcc cgcgccgttg
     5281 gtttcctggc caagataacc atgacttaat ggaatacgtt catccagttc gcgcgccggt
     5341 aattggccta cgcgcagttg ggtagtaacg aaatcagcat ctttcaatgc ttcgcggcga
     5401 tccagcgttt tataaagctt catcgggacg ccagcgttat caatcatccg ttggcagagt
     5461 tcaaaaatga tatccagttt ctctttacca tcttcgacat ccaccagcca taattcgctg
     5521 accggcaatt cgtgataacg cttaataaat ccttccagta actccggggt atagctgctc
     5581 ccgccaccaa tagtgacgac ttttaatttc tggctcataa tttctccctt cagtacagaa
     5641 tactgatatc tggcatatct gcccccccgg acataaataa tccagcaaca ggacagatat
     5701 gtgaattgtc aggtataacg acttactgat taaattcagt caatttctta cgatagctct
     5761 taggcgtaaa ggacgttaat ttcttaaacg ttttaataaa caaactcggg ctactataac
     5821 cggcctcaaa cgcaatatcc gtcacggaat agttggtcat ttccagttgt tttttggcaa
     5881 aattaatacg gatctcatta ataatctgca ttggtgtttt gccataatat cgttgagtcg
     5941 ctcgcgtcaa atattcctgt gatttggctg acaacgtcac catattctcc agcgccgatt
     6001 cactaaactg ctctttatca tgcatctttt ctaccgtact tttcagccac tgcggtacat
     6061 catcaatcac ctgttcttcg cggtaatgac gtaaacggtt aatgacataa aaagtaacca
     6121 tctcaacaaa ctcttccagc cctgtttcgc ggaaattcaa tgaagaaatc actgtttcca
     6181 cataggtgag aaaagcgtta ttggtccggt atacctgcga agcgacaaag caataaggca
     6241 acaacggcag gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc aatatacgcg
     6301 tggcaccaaa ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc
     6361 cccgttccag taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca
     6421 gagtaaattc ataatagtcg tgctgatgca gcccgctgat actctcagtt ttgttataga
     6481 taaacacatg gaagtttttg ccattaaaca actgctgttc tcgggcagtg gcaatttccg
     6541 gcgcgttaat cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca
     6601 attaattcag taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg
     6661 accagcacca gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc
     6721 tgtaccagat gcgcttcatt caatgccatc cgtgactgat ccatcatggc ttttgctgca
     6781 gcaaaatcgc cctgcttcgc ctgtttcagt gccgcatacg ccaggctgcg cgcttgtccg
     6841 gagttgatga taagccccat caccacttct tccagctctt cagcttccgt ttgcgtatcg
     6901 ggaatgttat cgagatccat catacatcgt tcctctctat tcttaccggc acgattaccc
     6961 gtaccggcat cgattaaaat ttcagggcgt tagcgatatc ttcttcgcgc tcttctttat
     7021 caatcgcgtt ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga
     7081 taccaaggtt gaagagtgca accagcaatg cggcgacgct accgttggtg ttaaagaagg
     7141 ctcccagacc ggttggcatg gtccacggtg caatattggt caccggagga atgatgccca
     7201 tgtagtacgc tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa
     7261 acatcaccgg gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga
     7321 tgccggacgg cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa
     7381 agatagccag gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca
     7441 gcatcggctt ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac
     7501 catattgctg ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca
     7561 gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca
     7621 cataggccca gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa
     7681 tgatctgatg gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg
     7741 aaagaataat aaagccggga attaatgccg agaaggaacg cgatactgaa gcaggtacgc
     7801 tatcgggcaa tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca
     7861 ccaggccaat aataatcccg gagatgatat tcgctccacc taaccagttt gcgccaacca
     7921 cataggcctc accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc
     7981 cagccgccag cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg
     8041 ccattaaaga cattatccct aatgttccgt tatatacgtt gccgccaata cctttcaggc
     8101 cattaagtgt ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaaacgccc
     8161 cctccccaaa gcttagaaaa acgttgttaa ttaatacaaa catggccccc gcaagggtta
     8221 acggcattaa gcgaataaag ccatttttga ttgcattaac gtgtggctgc tttcctattt
     8281 taactgcaaa agggaggagt accttttcaa gcgatgcaat aacattactc atagaaaaat
     8341 acccttaaaa accgcaattt aaatattgcg gtattgattt atgaaataac tctttgacgg
     8401 gaaaatttaa aataaattaa tttgctgcgg cttttttaat cgctgcaacc gcagccttaa
     8461 gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt
     8521 tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga
     8581 catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg
     8641 gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag gtagacatgc
     8701 ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta
     8761 tctgtcagcc agacactccg caagccttaa cctgcttcca tgctctgggt aacttgcgaa
     8821 accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat
     8881 ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg
     8941 ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg
     9001 gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg
     9061 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
     9121 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtacca
     9181 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
     9241 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttagtacga
     9301 tcataagccg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
     9361 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
     9421 ttgggcgctt tttcaagccc gcgaacgaaa cggctccgct ttcagaggat tcctgtatga
     9481 cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtggcaacg ggttagcttt
     9541 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
     9601 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtatcgatt
     9661 ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg
     9721 gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc
     9781 tggaaaccgg caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg
     9841 ccgacgaaca agattgccag gatgccattg cctttattca accggatcgc gtattaaccg
     9901 ttaatatcaa gggcgcggta ttggccagcg aacaggcatt gcgggaagca gacattgaac
     9961 tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca
    10021 ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg
    10081 gattcttcac taaatatggt gacggcggta cagacattaa tccgctgtat cgtctcaaca
    10141 aacgtcaggg taaacagtta ctggcggcat taggttgccc ggaacacctt tataagaaag
    10201 cgccaacggc cgatctggaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg
    10261 tgacctatga caatatcgac gactatctgg aagggaaaaa cgtacctgaa caggtcgcca
    10321 gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaccgttt
    10381 tcgatgattt ctggaaaaag taataatttg cacataatgg tttgtgcgaa aaagagtaat
    10441 ttgttcacgc cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa
    10501 tatattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt
    10561 catcgacccg cctctttttt aatcgctccc cgcctgttac actggataga taaccagcat
    10621 tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca
    10681 atttatgaat atcccgaaca tttacgttca ttccttaatg acttacccac ccgaccaggg
    10741 gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat
    10801 atccgcagtc gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa
    10861 tcccggcgaa ttagctggat ctgtaccgca ggcgaaatcg gcgctctgct ccttgaagcg
    10921 cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc
    10981 tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt
    11041 tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg
    11101 cagaccatcg ctgatgaaca aaaactttgt tatggcctgc tgggactgga accgttaagt
    11161 cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa
    11221 gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgggtggtg
    11281 tgttggcctt ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat
    11341 catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg
    11401 ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg
    11461 ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga
    11521 tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga
    11581 accagcacgc gccctggtcg gtagtgagca atttatcgcc cagttgccac gccccgttaa
    11641 tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga
    11701 ctttcgcctt acagacatcc aggcgtgtca tgatgttgaa atccatcgac atctgccctt
    11761 ccgtcagttt cgcttttacc acctggtccg ctgcaaaggc aaaaggctgt aacggtttta
    11821 aggtatggtt aaagcggtct gcgctttcaa ggagcatctc gccgccttcc agcaacgtca
    11881 ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc
    11941 tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac
    12001 cggcagcatt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg
    12061 ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact
    12121 cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga
    12181 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
    12241 gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc c
//
LOCUS       DAZPJP010000112        12148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPJP010000112 DAZPJP010000000
VERSION     DAZPJP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.112"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHU9J_002809"
     CDS             complement(<1..101)
                     /locus_tag="ACHU9J_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906305.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHU9J_002810"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHU9J_002810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1906306.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHU9J_002811"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHU9J_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1906307.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHU9J_002812"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHU9J_002812"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1906308.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHU9J_002813"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHU9J_002813"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1906309.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHU9J_002814"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHU9J_002814"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1906310.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHU9J_002815"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHU9J_002815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1906311.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHU9J_002816"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHU9J_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1906312.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHU9J_002817"
     CDS             9871..10449
                     /locus_tag="ACHU9J_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1906313.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHU9J_002818"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHU9J_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1906314.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         2685 a         3302 c         2889 g         3272 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgttcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttcccccc
//
LOCUS       DAZPJP010000113        12106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPJP010000113 DAZPJP010000000
VERSION     DAZPJP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.113"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            277..537
                     /locus_tag="ACHU9J_002819"
     CDS             277..537
                     /locus_tag="ACHU9J_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR1906315.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            728..868
                     /locus_tag="ACHU9J_002820"
     CDS             728..868
                     /locus_tag="ACHU9J_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1906316.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(1175..1474)
                     /gene="ihfA"
                     /locus_tag="ACHU9J_002821"
     CDS             complement(1175..1474)
                     /gene="ihfA"
                     /locus_tag="ACHU9J_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1906317.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(1479..3866)
                     /gene="pheT"
                     /locus_tag="ACHU9J_002822"
     CDS             complement(1479..3866)
                     /gene="pheT"
                     /locus_tag="ACHU9J_002822"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1906318.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(3881..4864)
                     /gene="pheS"
                     /locus_tag="ACHU9J_002823"
     CDS             complement(3881..4864)
                     /gene="pheS"
                     /locus_tag="ACHU9J_002823"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1906319.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(5073..5197)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(5147..5191)
                     /gene="pheM"
                     /locus_tag="ACHU9J_002824"
     CDS             complement(5147..5191)
                     /gene="pheM"
                     /locus_tag="ACHU9J_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1906320.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(5314..5670)
                     /gene="rplT"
                     /locus_tag="ACHU9J_002825"
     CDS             complement(5314..5670)
                     /gene="rplT"
                     /locus_tag="ACHU9J_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1906321.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(5723..5920)
                     /gene="rpmI"
                     /locus_tag="ACHU9J_002826"
     CDS             complement(5723..5920)
                     /gene="rpmI"
                     /locus_tag="ACHU9J_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1906322.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(6017..6559)
                     /gene="infC"
                     /locus_tag="ACHU9J_002827"
     CDS             complement(6017..6559)
                     /gene="infC"
                     /locus_tag="ACHU9J_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1906323.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(6563..8491)
                     /gene="thrS"
                     /locus_tag="ACHU9J_002828"
     CDS             complement(6563..8491)
                     /gene="thrS"
                     /locus_tag="ACHU9J_002828"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1906324.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            9016..10914
                     /gene="espL1"
                     /locus_tag="ACHU9J_002829"
                     /pseudo
     CDS             9016..10914
                     /gene="espL1"
                     /locus_tag="ACHU9J_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            11086..11193
                     /gene="yniD"
                     /locus_tag="ACHU9J_002830"
     CDS             11086..11193
                     /gene="yniD"
                     /locus_tag="ACHU9J_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1906325.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(11246..12004)
                     /locus_tag="ACHU9J_002831"
     CDS             complement(11246..12004)
                     /locus_tag="ACHU9J_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1906326.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQLLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         3153 a         3073 c         2724 g         3156 t
ORIGIN      
        1 aaaagttcta ttagcgcaca aatcaaaaca tgagggtgta ctcaaataat acaatgctat
       61 agtaaatcta taagtgcata actatttcct gatcttttcc caaaatagat gacaggataa
      121 aaaacattcc aatcttttac aggctttgga taaatcgtat caaagcctat ttcttcaaga
      181 gttaaatcac cgcttttcgc tcgaaacgga ctggctgttg gagtatgagc cagaacaact
      241 tagaatagcc cctgcactac gttaagggag gtcgctatgt tccgttgtcc gctttgtggc
      301 gcatctgccc gtatccgcac cagtcgtccg gaaaatgatt caaacaccgt gcggcaaaag
      361 tattaccagt gtaacaatct ggaatgcggc gtatgcttct caacactgga agctttccat
      421 aaattcacat caaaacacgc ctccggcgtt cactcttcag aagatatccc gtggcatgat
      481 ctgccagctt cacacagggg aaacaatcag atgagcttgc ctttacctca gaattaacag
      541 gcagaattgc cggagtaaca aaaaagcgat agattacgcg cgggtgcctt tcggctgatg
      601 gtcggaggga atacccgaag gccagatgtg gaaaggcccc ggaaaacatc tctgtttaac
      661 cgaggcccta accgcattac cttgacaagt gaaaggttag cgcctctccg gaaaaggagc
      721 aagtgctatg tcgcaaaaat cgcttacgac catcacgttc tgcgtgacgg caatcctcat
      781 catctggatg ttgcacggtt cgctgtgtga aatacggatg agcttctggg gagcggagtt
      841 tgcggcgttc ttacagtgta agcagtaagg aaaccgcgac gggggagcaa tcccccgtca
      901 atcggttgcc agggtaaggc cggataaggc accctatctc acagatatga acaacaaacc
      961 cgcagcgtaa aaactgcggg ttttcttttt ggtgccctca ccgacttgaa acagtgataa
     1021 gactataaga caaagcccgc aatgtagcgg gcttactccc ctcaccctcc actgaatgtg
     1081 gacacacagt ggaacgaagc ggcataagat attataaatc aatcagttat tgaaagaaaa
     1141 aaggccgcag agcggccttt ttagttagat cagattactc gtctttgggc gaagcgtttt
     1201 cgacccggct ttttaacttc tgcccgggtc tgaaggtcac cacgcgccgt gctgtaatgg
     1261 gaatatcctc gcccgttttc gggttacgtc ccgggcgttg attcttatca cgcagatcga
     1321 agttaccaaa accagagagt ttcacctgtt cgccgttttc cagagcgcga cggatctctt
     1381 cgaaaaacag ttcaaccagt tctttggcat cccgcttgct aagcccaagc ttatcaaaca
     1441 gatattctga catttcagct tttgtaagcg ccataggttc aatccctcaa tgatgcctgg
     1501 aatcgctctt ttaatgcctc tacacatttg gcgacggtag cggcaatctc ctcttcttcg
     1561 agtgtacggc tggtatcttg caggatcagg cttatggcga ggctcttata cccctccgca
     1621 acacccttac cgcggtacac gtcaaataag tttacgccaa ctacctgatt tacgccaact
     1681 ttcttacatt cggataaaat atccgctgcg ggaacgtttt ctgcgaccac caccgcgatg
     1741 tcacgacggt tcgccgggaa gcgagaaatc tcgcgcgcct gaggcaccac gcggtctgcg
     1801 agcttgttcc actccagttc gaacaccaga gtgcgaccgt taagatccag tttacgttcc
     1861 agttcaggat gaacaactcc aacaaaacca atacgttccc ctttcagata aatcgctgcg
     1921 gattgccccg gatgcagtgc cggattcgct tctgcacgga actcaacctc attcagttta
     1981 ccggtcaggt cgagaacgga ttcaagatcg cctttcaaat catagaaatc aacggtctct
     2041 tttgccaggt tccagtgctc ttcgtaacgg ttaccgcaaa tcacaccggc taacatcaga
     2101 tcctgacgaa tgcccaacgg tgcctgagta tctggtacga aacgcagacc gctttcgaaa
     2161 atgcgcacac ggttctgctg acggttctgg ttgtacacca cggttgccag caggccggtc
     2221 cacagagaaa gacgcattgc tgacatttca acagagatcg ggcttggcag cagtaaggct
     2281 tcaacgcctg gatggatcat ctgctgcact ttcggatcaa cgaagctgta ggtgatcact
     2341 tcctgatagc ctttgtcgtt gagcagcgtt ttcacgcgct tgagcgacag gtcagcttca
     2401 cggtgagtac ccataatcag gcttgcctgt accggctcat ccgggatgtt gttgtagccg
     2461 taaacacgcg cgacttcttc aaccagatct tcttcaatct ccatatcgaa acgccagctc
     2521 ggcgcaactg cctgccactc gtctttgcct tcggtcactt cgcagccgag acgacgcaga
     2581 atgtcagtta cctgttcatc agcaatatga tggccgatca ggcgatccag tttgctacga
     2641 cgtaaagtga tggttgcacg cttcggcagc gttgcttcgt tggtgatatc aattaccgga
     2701 ccagcctcac caccgcagat gtcgatcagc agacgggtcg cacgttccat cgctttgtgc
     2761 tgcagtgccg gatcaacgcc acgctcataa cggtgagacg catcagtatg caggccatga
     2821 cgacgagcac gaccggtgat agacagcggg ctgaagaaag cgcattccag caagacgttt
     2881 tgcgtttcgt cattcacgcc agagtgttcg ccaccaaaga tgccgcccat cgccagcgcc
     2941 ttgttgtggt cggcgatgac cagagtgtca gcattcagct tcgcttcagt gccatcaagc
     3001 agcaccagcg tttcgccctc ttttgccatc cgcaccacaa tgccgccttc aatgcgatct
     3061 ttatcgaaag cgtgcatcgg ctggcccagt tcgagcagta catagttggt gacgtcaaca
     3121 actgcatcga tagaacggat cccgcaacga cgcagttttt ctttcatcca cagcggagtt
     3181 ggcgctttaa cgttaatgcc ttttaccaca cggccaagat agcgcgggca ggcttcaggc
     3241 gcttcgactg taatcggcaa cgtgtcgtcg atggtcgcac caaccggaac gatttccggt
     3301 tcaaccagcg gcagctgatt cagcacggca acgtcacgcg caacaccaat gatacctaag
     3361 cagtcggcac ggtttggcgt tacgctgatt tcgatggtgt tgtcatcgag tttcaggtat
     3421 tcgcggatgt cagtgccaat cggcgcatct gcaggcagtt cgataatgcc gctgtgatcg
     3481 tcagaaattc ccagttcaga gaaggagcac agcatccctt cagacggttc gccacgcagt
     3541 ttcgccgctt taattttgaa atcacccggc agaacagcac caatggtagc taccgccaca
     3601 cgcaggccct gacggcagtt tggcgcacca cagacgatgt ccagcaggcg atcgccgccg
     3661 acattcactt ttgtcacacg cagtttgtca gcgttcggat gctgcgcaca ctcaaccact
     3721 tcaccaacga ccacgccgtg gaagctaccg gcaaccggtt ctacaccgtc aacttccagg
     3781 cccgccatag tgatttgatt cgccagcgca tcgctatcaa tcgccgggtt cacccattcg
     3841 cgtaaccaca gttcactgaa tttcataatc tattcctgcc ttatttaaac tgtttgagga
     3901 aacgcagatc gttttcgaag aatgaacgca ggtcggtgac gccgtaacgc aacatagtca
     3961 gacgctccat ccccatcccg aaggcgaaac cagagtaaac ttccgggtcg atgccaacgt
     4021 tacgcagcac gttcggatgc accatcccgc aacccagcac ttccagccat ttaccgtttt
     4081 tacccatgac gtcaacttct gcagaaggct cggtgaacgg gaagtaggaa ggacggaagc
     4141 gaatctgcaa atcttcctca aagaagttac gcaggaaatc gtgcagagtg cctttcaggt
     4201 tggtaaagct gatgttggta tcaacaatca gaccttccat ctgatggaac atcggcgtgt
     4261 gagtctggtc gtagtcgtta cgataaacgc ggccaggcgc gatgatacga atcggtggct
     4321 gctgggcttt catggtgcgg atctgtacgc cagaggtctg ggtacgcagc aggcgggtag
     4381 tgtcaaacca gaaagtgtcg tggtcagcgc gcgccgggtg gtgaccagga atgttcagag
     4441 catcgaagtt atgataatcg tcttcgattt ccggcccggt tgccacggta aagccaagct
     4501 caccgaagaa actttcgata cggtcgatgg tacgggtaac cggatgcaga ccgccgtttt
     4561 caatgcgacg acctagcagg gagacatcaa tcgtttccgc cgccagacgc gcattcagcg
     4621 ctgcgctttc cagttccgct ttacgcgcat tcagcgcctg ctgaacctgc tctttcgctt
     4681 cgttgataac cgcaccagct gccggacgct cttctggcgg cagctcacgc agggtcgtca
     4741 tctgaagggt taagtgccct tttttaccca aatattcgac gcgcacatta tctaacgcgg
     4801 caacatctga cgcctggcta atggccgcct tcgcactggc aaccagttct gcgagatgtg
     4861 acatggtttt cctcattgtg tcagtggtga cactggttcg ttggacttag agcctatccc
     4921 atcaggctat tttacttgcc attttggtcc cgggcagtgc tcgaaattct cacgtactta
     4981 aatacgctcc ggtttctccg cgctggccgt gtccagactg gctgcgacaa ttacgcctga
     5041 tagactggct ttttattttt caaaacgcgc atacaaaaaa agcctccact gggaggcttt
     5101 caggcgctgt tttccgtttc tcttctcacg cgctagcctc ctggattcag gtgctaaagt
     5161 aaaaaaagaa gcggaaaata gcagcattca ttgcttgcgt taccttttgg tactcttcaa
     5221 aagaccttta ttgaaaaggc tacggcgata aaagtcaatg ttttgatggc gttgaaacga
     5281 aaagagggag actagctccc tctttcaact ggcttatgcc agagctgctt tcgctttttc
     5341 aaccagagcg gtgaacgcta ctttgtcgaa tactgcgata tcagccagga tcttacggtc
     5401 gatttcaaca gaggcttttt tcaggccatt gatgaatttg ctgtaagaaa taccgttctg
     5461 acgtgctgct gcgttgatac gcgcaatcca cagttgacgg aactgacgct tacgttgacg
     5521 acggtcacgg taagcatact gaccagcttt gataacagcc tggaaggcaa cgcggtatac
     5581 gcgagaacgc gcaccgtagt agcctttagc ttgtttcaaa attttcttgt gacgtgcgcg
     5641 tgcaataaca ccacgtttta cgcgagccat atgtgctctc ctgtatctat attctaatta
     5701 aaaagttaaa aacgttaacg gcttatgcgt acggcaggca cgcgattacc aggcccagat
     5761 cgcctttgga aaccatggct ttcggacgca ggtgacgttt acgtttggtc gcttttttgg
     5821 tcagaatgtg acgcaggtta gcgtgcttgt gcttaaaacc acctttaccg gtttttttga
     5881 agcgcttagc agcaccgcgt acggtcttaa tttttggcat tttaataact tccacttcgc
     5941 attgttaata aacgaaacaa aggcgaacaa agcctgtgaa gcccgaaggc tccacagaca
     6001 gtgctacttg aaggccttac tgtttcttct taggagcgag caccatgatc atctggcggc
     6061 cttcgatctt cgttgggaag gattcgacca ctgccagttc ttgcaaatcg tctttcacgc
     6121 gattaagcac ttccataccg atttgctggt gcgccatctc acgaccgcgg aaacgcagcg
     6181 tgattttggc tttatcaccc tcttcgagaa agcgaatcag gctgcggagt tttacctgat
     6241 agtcgccttc atctgtacca ggacggaatt taatttcctt aacctggata actttttgct
     6301 ttttcttctg ttccttagaa gacttgctct tttcatagag gaatttgccg taatccatta
     6361 tacgacaaac cggcggctcg gcgttagggc tgatctcgac taagtctact ccggcttctt
     6421 ctgctttctc cagagcttct ctcagactca caataccaag ctgctcgcct tccagacctg
     6481 ttaagcgaac ttcctgggcg cgaatttcgc cattgatacg gttagggcgc gccgtttgaa
     6541 ctcgttttcc gcctttaata ccttattcct ccaattgttt aagactgcgg ctgcgaatct
     6601 cttgttgcag cttctcgatc acttcattta cgtccatgct tcccaggtct ttaccacggc
     6661 gggtgcgaac ggcaactttg cctgattcca cctctttatc accacagacc agcatatatg
     6721 ggacgcgacg caaagtgtgc tcgcggattt taaagccaat cttctcattt ctcaagtctg
     6781 ctttaacacg aatgcccgca tttgatagtt tttgcgtcaa ttcgttaacg tattcagact
     6841 gtgaatcggt aatattcatg ataacaacct gaactggcgc aagccaggtc gggaagaaac
     6901 cagcgaattc ttcggtcagg ataccgatga aacgttccat cgaccccaga attgcgcggt
     6961 gaatcattac cggtacctta cgctcgttgt cttcgcccac ataggaggcg ctcagacgag
     7021 acggcaagga gaagtccagc tgtactgtac cgcactgcca tgcacgatcg aggcagtcat
     7081 acagggtaaa ttcaattttc ggaccgtaga aagcgccttc acccagttga tattcaaacg
     7141 ggatgttgtt ttcttccagc gcaaccgcca ggtccgcctc agcacgatcc cacatttcgt
     7201 cgctgccaat acgtttttca ggacgagtgg agagtttgac gacgatcttc tcgaagccaa
     7261 aagtgctgta catatcatag actaaacgga tacatccgtt aacttcatcg cgaatttgtt
     7321 cttcagtaca gaagatatgc gcgtcatcct gggtaaatcc acgcacgcgc atcaggccat
     7381 gcagcgaacc tgacggctcg ttacggtggc agctaccaaa ctcggccata cgcagcggca
     7441 gatcgcgata agacttcagc ccctggttga aaatttgtac gtgacccggg cagttcatcg
     7501 gcttaatgca gtattcacgg ttctcagaag atgtggtgaa cattgcatct ttgtagttgt
     7561 cccagtgacc ggttttttcc cacaggacac ggtccatcat gaacggacct ttaacttcct
     7621 gatactggta ctctttcagt ttagaacgaa caaacacttc cagttcacgg aagatggtcc
     7681 agccgtcgtt gtgccagaat accatacccg gcgcttcttc ctgcatatgg tacaggtcga
     7741 gctgtttacc gattttacgg tggtcgcgtt tcgcggcttc ttccaggcgc tgcaggtaag
     7801 cgttaagtgc ttttttgtct gcccacgccg taccgtaaat acgttgcaac attttgttgt
     7861 tgctgtcgcc acgccagtaa gcccctgccg ttttcattag tttgaaatga tggcagaaac
     7921 gcatgttcgg tacgtgcgga ccgcggcaca tatcgacata ttcttcatgg aagtacagac
     7981 ctggcttgtc atcatgggcg atgttttcgt caagaatgga gactttgtag ctctccccac
     8041 ggttggcgaa agtttcacgc gcttcgtgcc agctgacttt cttcttaatg acgtcgtagt
     8101 ttttctcagc aagctcatgc atccgcttct cgagtgcttc gacatcttcc tgggttaacg
     8161 tgcggtcaag atcaacgtcg taataaaaac cgttgtcaat aaccgggccg attgccattt
     8221 tggtatgcgg ccaaagttgt ttaatcgcat gccctaacag gtgcgcacag gagtgacgaa
     8281 tgatctccag accttcttcg tctttggcgg taatgatcga cagttgtgcg tcgttttcaa
     8341 tcagatcgca agcatcaacc agttcgccat taacgcgccc tgcgatacag gctttcgcca
     8401 gacctggacc aatgtccagc gcaacatcca tggggcttac agcgtggtcg taatggcgtt
     8461 ggctgccatc aggaagagtt ataacaggca ttttatatcc ttaatttgca gtggtgaccc
     8521 acacgaaaga tcacatacaa agaaaaattt gtttattaac agttcattgc gaaaccatct
     8581 agccaacaaa tgctaaattg gttcgcaatc aggtacacaa atgaaagaca ttaacctctc
     8641 accttccacc cggttgaacg atgttaacac taaaaaaagg gagattgtac ctttccgttt
     8701 cacattgatt cgattcgcaa ttcgtttgta gtgagtacac cagcaaaacg cttaaagaac
     8761 cactgataac atcacggtgc gtctgaaagg tttactgcga gcggatttac agcagcataa
     8821 actgaatcca gcaaagttcg ttgcgtgatt tggcggtagt attttaaacg ctcatgagat
     8881 taatgacgaa gtggtcatat cacaatgata aaagtgacac aattcttata gcaatttttc
     8941 gtgcacattt cgttctggcg ataataatta atcatcatcc tcaaaacctc cagacatata
     9001 taaggcgaat agattatgag tcaaaacgat atcattatca gaacccatta taagtctcct
     9061 catagattgc acatcgatag cgacatacca acgccttcat cagagcctat taatcaattt
     9121 gcgcgccaac tcatcaccct acttgatacc tctgacttaa gttcgatgct gtcatactgt
     9181 gttactcagg aatttaccgc aaactgtcga aaaatttcac aaaattgtta ttccactgcc
     9241 ctttttatca ttaactttgc cacttcaccc atccatgcag aaaatatact cattacatta
     9301 cactataaaa aagaaatcat tcccttattt ctggaaacca cgcctattaa ggctaaccat
     9361 ttgcgaagca tactggatta tattgaacag gaacagttaa ctgccgaaga tcgtaaccat
     9421 tgtatgaaac tgtctaaaaa aatccataga gaaaaaacta tacaaccaac agtaaatctc
     9481 aatggtagtg catttttttc gcaatctcct tctgacgcta ttttttgtcg ccatctgtca
     9541 ttgcaatacg cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacac
     9601 tactcctccg ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga
     9661 gcattacttc gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc
     9721 gctttagata ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt
     9781 ttatatatag acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga
     9841 caattaaatt atgttgtgtc tgtttacgac ccgaacgata ccaacgttgc cgtcagaggc
     9901 acccacaggg cagcacgggg ctttctctcg cttgataagt tcatcagttc agctcccgat
     9961 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct tcctctatta
    10021 cctgagggag ttccagttgc tattttagcg ggtatcacga cacgaatgcc atttgcccct
    10081 atacatccat cggcaatgtt gttaataatg gccacaggcc agactcaata gcttattaca
    10141 ctattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
    10201 aatagcgttg gtacacctgc tttatttctg gctatgatga acggacaaac tgacaacgta
    10261 aaaatattta tgcaagaaat tcagtcactg gtatacaatc atatcattca tgaagataat
    10321 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggactcta tatctccatg
    10381 ttgtatggat tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca
    10441 caagaacttt taaacaaaaa aatggtgatg agtattttag ccatgaaaat acatgatggt
    10501 gagccaggat tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac acggttcctc
    10561 tctaaaatta atggcatcgc ctttaaatac aagttgagca aagctaacat catggattta
    10621 ttaaaaggag ctacagcaca gggaacccct gcattataca tcgccatgag caagggtaat
    10681 gaagacgtcg tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa acattctttt
    10741 agtcaacatc agttatttac actattggcc gctaaaaatc atgacaacat gtcagctgtt
    10801 catatagcca ttcatcataa gcattataaa actgtagaaa catattatgc tgctataaat
    10861 gtcatcagcc aaagcctgag ttttagtgct gatgaaataa agacgtattt ataaccagct
    10921 atttacgata tatatcgata cataatttta tttttttcat aaaacgcaga aacagtgacc
    10981 atgtgcacaa cgccacccga cggctgtaat aacaaaaaat cccttgatgc ctatcccttt
    11041 tgttacactc cgttatcacg cacaagagat atgcaggaca ctggtatgcc gactaaacgc
    11101 tttgataaaa aacactggaa gatggtggtg gtgctactgg caatctgtgg cgctatgttg
    11161 ttgctacgtt gggcagcaat gatttggggc tgagcagtga caaacgaaga cagccagacc
    11221 gcataacacg gtccggcaga taattttaca tgctgtagcc cagcgataag gttgtccgac
    11281 gatcggtatg ttccggtgca gactctggag gttcagagtt ccatgtgacg ttgtaagcca
    11341 ccttcagtcc aaaatgttca ttaatagcaa catttaatgc gctttcggag ttcagcgttg
    11401 tatcttccgc gccaaagacc gaaacaccct gcgtaaattt agcgttgtca gtcaactgcc
    11461 atgcataagc cccggaggca tagcccagca gctgggtttc actggcattg tcggtatatt
    11521 tgtcgtaacg cacacctggg ccgaattcaa agcggaaact gtgtaccggg ccattcaaaa
    11581 actggcgacc ataacccgcg gtcaacacat cgcgctctcg atagccgtta taacggtctg
    11641 tcagccagct tgcctgacca aataaatagt cataatcagt taaattaaaa cggctacgcc
    11701 cgcccgccgc atatttttct gaagaacgct catcattaga agaagtatta ctggcgttcc
    11761 cccacagcga ccaggcagtg gtttgtccat accaggtcat ggtggtgtca gccgtaagtg
    11821 aggagctttt cgtattgcct gattgtgcaa gatatcctgc gttcagatta ccttcgaaag
    11881 gttttttagc gctggcagga tcgtccatga cagtaaaaac ggaatcatcg gcagctgcat
    11941 tcagtgacgc aaacatgccc cccgccaaca taacgatggc aggaactgtc ttcaaaagct
    12001 tcatttatca agagtccgta caacaaaaaa agagaccatc gcggtcccgg aaactttctt
    12061 aaggatcaaa gattagcgtc cctggaaagg taacgaatta taaaaa
//
LOCUS       DAZPJP010000114        12080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPJP010000114 DAZPJP010000000
VERSION     DAZPJP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.114"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHU9J_002832"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHU9J_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1906327.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHU9J_002833"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHU9J_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1906328.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHU9J_002834"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHU9J_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1906329.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHU9J_002835"
     CDS             complement(3668..3898)
                     /locus_tag="ACHU9J_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1906330.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHU9J_002836"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHU9J_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHU9J_002837"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHU9J_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR1906331.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHU9J_002838"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHU9J_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHU9J_002839"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHU9J_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR1906332.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHU9J_002840"
     CDS             7629..8642
                     /locus_tag="ACHU9J_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1906333.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHU9J_002841"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHU9J_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHU9J_002842"
     CDS             9533..10327
                     /locus_tag="ACHU9J_002842"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1906334.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHU9J_002843"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHU9J_002843"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1906335.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="ACHU9J_002844"
     CDS             11983..>12080
                     /locus_tag="ACHU9J_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR1906336.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2946 a         2914 c         3223 g         2997 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAZPJP010000115        12024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPJP010000115 DAZPJP010000000
VERSION     DAZPJP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.115"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            180..1289
                     /gene="livK"
                     /locus_tag="ACHU9J_002845"
     CDS             180..1289
                     /gene="livK"
                     /locus_tag="ACHU9J_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1906337.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1337..2263
                     /gene="livH"
                     /locus_tag="ACHU9J_002846"
     CDS             1337..2263
                     /gene="livH"
                     /locus_tag="ACHU9J_002846"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1906338.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2260..3537
                     /gene="livM"
                     /locus_tag="ACHU9J_002847"
     CDS             2260..3537
                     /gene="livM"
                     /locus_tag="ACHU9J_002847"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1906339.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3534..4301
                     /gene="livG"
                     /locus_tag="ACHU9J_002848"
     CDS             3534..4301
                     /gene="livG"
                     /locus_tag="ACHU9J_002848"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1906340.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4303..5016
                     /gene="livF"
                     /locus_tag="ACHU9J_002849"
     CDS             4303..5016
                     /gene="livF"
                     /locus_tag="ACHU9J_002849"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1906341.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5300..6364
                     /gene="ugpB"
                     /locus_tag="ACHU9J_002850"
                     /pseudo
     CDS             5300..6364
                     /gene="ugpB"
                     /locus_tag="ACHU9J_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6462..7349
                     /gene="ugpA"
                     /locus_tag="ACHU9J_002851"
     CDS             6462..7349
                     /gene="ugpA"
                     /locus_tag="ACHU9J_002851"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1906342.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7346..8191
                     /gene="ugpE"
                     /locus_tag="ACHU9J_002852"
     CDS             7346..8191
                     /gene="ugpE"
                     /locus_tag="ACHU9J_002852"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1906343.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8193..9263
                     /gene="ugpC"
                     /locus_tag="ACHU9J_002853"
     CDS             8193..9263
                     /gene="ugpC"
                     /locus_tag="ACHU9J_002853"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR1906344.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9260..10003
                     /gene="ugpQ"
                     /locus_tag="ACHU9J_002854"
     CDS             9260..10003
                     /gene="ugpQ"
                     /locus_tag="ACHU9J_002854"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1906345.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(9990..10430)
                     /locus_tag="ACHU9J_002855"
     CDS             complement(9990..10430)
                     /locus_tag="ACHU9J_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1906346.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10550..>12024
                     /gene="ggt"
                     /locus_tag="ACHU9J_002856"
     CDS             10550..>12024
                     /gene="ggt"
                     /locus_tag="ACHU9J_002856"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1906347.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVL"
BASE COUNT         2616 a         3087 c         3468 g         2853 t
ORIGIN      
        1 agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcactg ttatcacaaa
       61 atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt
      121 ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta
      181 tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg
      241 ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt
      301 ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg
      361 gaattaaggg cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag
      421 ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt
      481 gttcttcttc tacccaacct gcatcagata tctacgaaga cgaaggtatt ctgatgatct
      541 cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg
      601 ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc
      661 cccagcgcat cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg
      721 tgcaggacgg gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg
      781 gggagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt
      841 actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc
      901 tgaaaactca gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgctg
      961 gcgatgctgc cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa
     1021 accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct
     1081 ggatcaccta cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg
     1141 agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc
     1201 tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg
     1261 ccgatggttc atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt
     1321 ttagaaaggt taccttatgt ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg
     1381 cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat
     1441 tatcggcatg atcaacttcg cccacggcga ggtttacatg attggtagct atgtctcctt
     1501 tatgatcatc gccgcactga tgatgatggg cattgatacc ggctggctgc tggtggcggc
     1561 gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc
     1621 ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc
     1681 catcttcctg caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag
     1741 tctgtttaac ggtcagtggg tggtggggca tagcgaaaac ttctctgcct ctattaccac
     1801 catgcaggca gtgatctgga ttgttacttt cctcgccatg ctggcgctga cgattttcat
     1861 tcgttattcc cgtatggggc gcgcctgccg tgcctgcgcg gaagatctga aaatggcgag
     1921 cctgcttggc atcaacaccg accgggtgat tgctttgacc tttgttattg gcgcggcgat
     1981 ggcggcagtg gctggcgtac tgctcggtca gttctacggc gtcattaacc cctatatcgg
     2041 ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc
     2101 aggggcgatg attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct
     2161 gagtacggaa tataaagatg tggtctcatt cgccctgctg attctggtgc tgctggtgat
     2221 gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca
     2281 atggcactgc tctctgccgc gatgttcttt gtcctcgctg gcgtctttat gggcgtgcaa
     2341 ctggagctgg atggcaccaa actggtggtc gacagcgctt cggatgtccg ttggcagtgg
     2401 gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgacctgc tttccagaaa
     2461 gggctaaaaa gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg
     2521 aagcagaaac tgttcctcgt ggcgctgctg gtgcttgcgg tgtcgtggcc gtttatggtg
     2581 tcacgtggga cggtggatat cgccactctg accatgatct acattatcct cggtctcggg
     2641 ctgaatgtgg ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg tttttacgcc
     2701 atcggcgctt acacttttgc gctgctcaat cactattacg gtctggggtt ctggacctgt
     2761 ttgccgattg ccggactgat ggcagcggcg gcgggcttcc tgcttggttt tccggtgttg
     2821 cgtctgcgcg gtgattatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata
     2881 ttgctgctca ataacaccga aattaccggc ggcccgaacg gtatcagtca gatcccgaaa
     2941 ccgactttct tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc
     3001 agtaatttct ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg
     3061 gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg
     3121 gggcgtgcgt gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc
     3181 ccgcgtcgta tcaagctgac tgcctttacc atcagtgccg cgtttgccgg ttttgccgga
     3241 acgctgtttg ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg
     3301 gcgtttgtac tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg
     3361 gcggcaattt tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg
     3421 ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc
     3481 atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc
     3541 agccattatt atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca
     3601 acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg
     3661 gaaaaactac ggtttttaac tgtctgaccg gattctacaa acccaccggc ggcaccattt
     3721 tactgcgcaa tcagcacctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg
     3781 tgcgcacctt ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg
     3841 tggcgcagca tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt
     3901 tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt
     3961 tgctggaaca cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg
     4021 agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag
     4081 gtcttaaccc aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc
     4141 acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc
     4201 gaatttatgt ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta
     4261 ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg
     4321 tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg
     4381 catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc
     4441 ttgctcggca cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat
     4501 aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg
     4561 gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt
     4621 tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gttcccacgt
     4681 ctgcatgagc gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg
     4741 gcgattggtc gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctt
     4801 ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag
     4861 gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc
     4921 ggctacgtgc tggaaaacgg ccatgtggtg ctctccgata ctggtgatgc actactggcg
     4981 aatgaagcgg tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc
     5041 aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag
     5101 gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt
     5161 ttacgccgca tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat
     5221 ttttccgctt atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt
     5281 catctttctg acaccttact tgcaggttta tgaagttggc accgccacca tgatggcatc
     5341 gaaagccatt aaaccggtat atgacgtgtt taaagaggca gggattcaat tcgatgagtc
     5401 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
     5461 ccagccattc aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc
     5521 ggggttagac ccggaacagc cgccgaaaac ctggcaggat ctggcggact cttccgcaaa
     5581 actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact
     5641 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
     5701 cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacattg ccatgctcga
     5761 agagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa
     5821 gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg ctaatattcg
     5881 tgagtacgcc aaatttaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga
     5941 tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaaaataa
     6001 agaaacgtat accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc
     6061 cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg
     6121 tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa
     6181 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
     6241 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
     6301 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
     6361 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg
     6421 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
     6481 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
     6541 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gcggcaaagc gtcgatccgt
     6601 ttggtttctc cagccagttt gtcggtctgg ataacttcgt cacgctgttc catgacagct
     6661 actatctaga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
     6721 tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct
     6781 atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga
     6841 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg
     6901 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcggtgt
     6961 ggaagcaaat cagctacaac tttctgttct tctatgccgc gctgcactcc attccccgtt
     7021 cgttgattga agccgcagct attgacggcg cagggccgat tcgccgcttc tttaagattg
     7081 ctctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct
     7141 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
     7201 ctacgctgat ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg
     7261 ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct
     7321 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
     7381 ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt
     7441 cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac
     7501 acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc
     7561 gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat
     7621 taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct
     7681 cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac
     7741 ggtggaagtc atcgccaacc tgaaaatgct cgacagctac gccggtttaa cgctgccgct
     7801 gatggcctcg gcgaccgcca cgtttctgtt ccgccagttc tttatgaccc taccggatga
     7861 attggtggaa gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt
     7921 ttttccgttg tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg
     7981 gaatcagtat ttgtggccgt tgttgattat taccgacgtt gatctcggca ccaccgtggc
     8041 agggatcaaa gggatgatcg ctacaggcga aggcaccacg gaatggaact cggtaatggc
     8101 ggcgatgttg ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt
     8161 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
     8221 ccaaaagctg ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg
     8281 gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg
     8341 ttgccgggct ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgaccg
     8401 aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaattacgcg ctttatccgc
     8461 atatgagcgt agaggagaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
     8521 aaattgccga gcgcgttaaa gaagcggcgc gcattctgga actggacggt ctgcttaagc
     8581 gtcgtccgcg tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg
     8641 tgcgcgatcc ggcggtgttc ttgtttgatg agccgctctc taacctcgat gccaagctgc
     8701 gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct
     8761 acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg
     8821 gtggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
     8881 ttgtggcgag ttttatcggc agcccggcga tgaacctgct gacaggccgc gtgaataatg
     8941 aaggcacgca tttcgaactg gacggcggta tcgtgctacc gctaaacggt ggctaccgtc
     9001 agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatatagcg ctaagctcgc
     9061 aggcagaagg tggtgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
     9121 tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc
     9181 cgacggcagg cagcacgctg tggctgcatt tggcggaaaa tcagctgcat ctttttgatg
     9241 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg
     9301 cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
     9361 tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca
     9421 tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
     9481 ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct
     9541 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga
     9601 aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg
     9661 ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga
     9721 agctgcacaa caggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga
     9781 cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
     9841 actcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac
     9901 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
     9961 cgatgcgatt gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca
    10021 ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctttccgg cagcatatgc
    10081 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg
    10141 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    10201 gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt
    10261 tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc
    10321 tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    10381 gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct
    10441 cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt
    10501 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    10561 gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc
    10621 cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt
    10681 acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga
    10741 tatcctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc
    10801 ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgttaa tccgctcgaa
    10861 aaatggcaat accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga
    10921 tatgttcctc gatgatcagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc
    10981 ttccggcaca ccgggtacgg tagcaggttt ctcgctggcg ctggataaat acggcaccat
    11041 gccgctgaac aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt ttatcgttaa
    11101 cgacgcgctg gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa
    11161 cagtaaagct atcttctgga aagagggcga gccgctgaaa aagggcgaca cgctggtgca
    11221 ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa
    11281 aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa
    11341 agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg
    11401 gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct
    11461 caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca
    11521 aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc
    11581 ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc
    11641 cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc
    11701 gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt
    11761 ggcggtgacc tatacgctga acaccacctt cggtatgggc attgtcgcgg gcgagagcgg
    11821 tattctgctt aataatcaga tggatgattt ctccgccaaa ccgggcgtac cgaacattta
    11881 cgggctggtg ggcggtgatg ccaacgccgt cgggccgaat aaacgtccgc tgtcatcgat
    11941 gtcaccaacc attgtggtga aagacggtaa aacttggctg gttaccggta gtcctggcgg
    12001 gagtcggatt attaccaccg tgct
//
LOCUS       DAZPJP010000116        11800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPJP010000116 DAZPJP010000000
VERSION     DAZPJP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.116"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            29..595
                     /locus_tag="ACHU9J_002857"
     CDS             29..595
                     /locus_tag="ACHU9J_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1906348.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            751..2783
                     /locus_tag="ACHU9J_002858"
                     /pseudo
     CDS             751..2783
                     /locus_tag="ACHU9J_002858"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2760..3104
                     /locus_tag="ACHU9J_002859"
     CDS             2760..3104
                     /locus_tag="ACHU9J_002859"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR1906349.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3119..3850
                     /locus_tag="ACHU9J_002860"
     CDS             3119..3850
                     /locus_tag="ACHU9J_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1906350.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3847..4908
                     /locus_tag="ACHU9J_002861"
     CDS             3847..4908
                     /locus_tag="ACHU9J_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1906351.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5060..6106
                     /locus_tag="ACHU9J_002862"
     CDS             5060..6106
                     /locus_tag="ACHU9J_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1906352.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6103..6894)
                     /gene="nei"
                     /locus_tag="ACHU9J_002863"
     CDS             complement(6103..6894)
                     /gene="nei"
                     /locus_tag="ACHU9J_002863"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1906353.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6930..7664)
                     /gene="pxpA"
                     /locus_tag="ACHU9J_002864"
     CDS             complement(6930..7664)
                     /gene="pxpA"
                     /locus_tag="ACHU9J_002864"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1906354.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7654..8586)
                     /gene="pxpC"
                     /locus_tag="ACHU9J_002865"
     CDS             complement(7654..8586)
                     /gene="pxpC"
                     /locus_tag="ACHU9J_002865"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1906355.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8580..9236)
                     /gene="pxpB"
                     /locus_tag="ACHU9J_002866"
     CDS             complement(8580..9236)
                     /gene="pxpB"
                     /locus_tag="ACHU9J_002866"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1906356.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9259..10002)
                     /gene="ybgI"
                     /locus_tag="ACHU9J_002867"
     CDS             complement(9259..10002)
                     /gene="ybgI"
                     /locus_tag="ACHU9J_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1906357.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10273..11754
                     /gene="dtpD"
                     /locus_tag="ACHU9J_002868"
     CDS             10273..11754
                     /gene="dtpD"
                     /locus_tag="ACHU9J_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1906358.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2818 a         3156 c         2896 g         2930 t
ORIGIN      
        1 tttttctttt ctgttaaagg actcagttat gtttaaagga caaaaaacat tggccgcact
       61 ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat
      121 tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa
      181 agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
      241 cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca acggttccgg
      301 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
      361 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa
      421 taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac
      481 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
      541 tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt
      601 actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat
      661 atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc
      721 gaattcaacc ttaatgttct cgataaatcg atgcgcgacc gcattgatat ttcattatta
      781 aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac
      841 caaatcagta acgggcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc
      901 atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcgttacca
      961 ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa
     1021 gccaatcagc aactgaatat tagtattccg caagcctggc tggcgtggca ctcagaaaac
     1081 tggaccccac cctccacatg gaaagaaggt gtcgccggta tcctgatgga ttacaacttg
     1141 tttgccagca gctaccgccc acaggacggc agcagcagca ctaacctgaa cgcctacggt
     1201 accaccggaa ttaacgccgg ggcatggcgc ttacgtagtg attatcagtt gaatcagact
     1261 gatagcgatg ataaccatga acagtcaggc ggaatatcgc gcacctatct ttttcgtcca
     1321 ttaccgcaat taggctctaa attaaccctc ggcgaaacgg attttagttc caatattttc
     1381 gacggttttt cttataccgg cgcggcactg gcaagtgacg agcgaatgtt gccatgggaa
     1441 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
     1501 agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac
     1561 gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg
     1621 gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgtca gggacaggtt
     1681 cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa
     1741 acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacggcggc
     1801 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca aaatatgctg
     1861 tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga tactcagcag
     1921 gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc cactaacagc
     1981 acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat
     2041 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttattg
     2101 tgggccaacc gattacccca ctaaacctca atctttacgc caacctgcta catcaaacct
     2161 ggtggaatgc agacgcctcc acgaccgcca acatcacggc aggttttaat gttgatattg
     2221 gtgactggag agatatctct atttcgacgt cattcaatac aacccattac gaagataaag
     2281 atcacgacaa ccagatttac ctgtcgattt cgctcccctt cggtaacggt ggtcgggttg
     2341 gctatgacat gcaaaacagt agccacagca ccacacaccg catgtcgtgg aacgatacgc
     2401 tggatgaacg taatagctgg ggcatgtctg ccggactgca atccgaccgt cctgacaatg
     2461 gagcccaggt gagcggtaac tatcagcacc tgagttcagc gggtgagtgg gatatttctg
     2521 gtacctatgc cgccaatgat tacagttccg tcagcagcag ctggagcggt tctttcaccg
     2581 caacccaata tggtgcagcg tttcatcgcc gcagctccac caatgaacca cgcctgatgg
     2641 tcagcaccga tggcgtggca gatattccgg ttcagggcaa tctcgactac accaaccatt
     2701 ttggcattgc ggtggtgccg ttgatttcca gttaccagcc ttccaccgtg gcggtgaaca
     2761 tgaatgactt acccgacggc gtaacagttg cagaaaacgt tatcaaagaa acgtggattg
     2821 aaggcgcgat aggttacaaa tcactggctt cccgttccgg taaagacgtt aacgtcatca
     2881 ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga tatccgccag gatgacagcg
     2941 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
     3001 atcaaaagtt taccgtggtc tggggtgata gccagcattg ctcgctccat cttcctgaac
     3061 atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg aaatgaaaat
     3121 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
     3181 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
     3241 ggtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
     3301 aggggagaaa agcgatgatc ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa
     3361 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
     3421 cgaaacgcta tttttctaca atatgcgtga aattcctccc gccccggaaa aaaacagcga
     3481 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtgttctggc gaccggctgc
     3541 attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc agcagggcaa
     3601 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctacc tgggacgaaa
     3661 tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa
     3721 cacaaacacc ggaaattata gcggcagcca gttttatctc ggttatatgg atgattacgg
     3781 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc catttacaag ccgtggaggc
     3841 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctgttatt tctgccgttg
     3901 aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg gtggctcggt agaaaaatca
     3961 gaagcgattc aaccttttgc tgtaccaggc aatgccaaac tcggcgataa gatttgggaa
     4021 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ttttgaaagt
     4081 gagcatgttt atgcctgggt aaccccctac ccgggggtgc aggatcgcta ttatcaactc
     4141 ggcgtaactt acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact
     4201 aaccagtgta ttgacagtaa gaacattgac atttataccc ctgagcagat tatcgcgatg
     4261 ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt
     4321 ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt
     4381 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
     4441 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
     4501 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
     4561 ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
     4621 aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg
     4681 gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa
     4741 aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg
     4801 ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag
     4861 gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg
     4921 ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg
     4981 attgctgtta ttttttcctg gcccggcttt ctttatgctg tatcgataca ccaaagagaa
     5041 aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact
     5101 tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat
     5161 gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt
     5221 tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct
     5281 caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc
     5341 cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc
     5401 ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc
     5461 agatattcgc ctggttgcat ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc
     5521 tgttctggtc acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt
     5581 ctggttcccg ccagtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg
     5641 tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg
     5701 cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat
     5761 ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg
     5821 ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc
     5881 gggtatggcg tgggggctga cccggtttat gcacattgat tttatgactg cctacctcgc
     5941 caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat
     6001 ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc
     6061 cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagccagg
     6121 gcaccagtaa aacgggcgag atgacagcgt ggttttctca atgatgctgc cacagcgttc
     6181 gcacggttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt
     6241 ctcatccacc tgccccctcg tagcgtagga aaatcgagga atatccagta acgcgtgtgc
     6301 gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac
     6361 ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag
     6421 cagtaatcct gcaaactgac ggttacgaaa gcgcggtgac aataaccgtt cctttaccac
     6481 ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt
     6541 ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt
     6601 gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc
     6661 aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaagt cgttggaaaa
     6721 atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg
     6781 tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt
     6841 gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat
     6901 atcctttttt cattctttat taatcggggt tatgctgcga caacaatctc cttttcggca
     6961 aatgtagagc gtagtcggcg ggcgaacgcc agtgcgtgct cgccgtcgcc atgcaggcag
     7021 actgtttgcg ccgtgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc
     7081 accatctcta gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctcgctga
     7141 cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc
     7201 gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga
     7261 atcaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct
     7321 ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc
     7381 gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttct
     7441 ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg
     7501 ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatgat ttgcgcatcg
     7561 cctgcatgaa atccacaggc aatattggca gaggaaacca gcgttaatag ctctgcgtcg
     7621 ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacac
     7681 taattgttcg aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca
     7741 ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc
     7801 aatgatacag gcaatacgcg ggtaaccatc ggtggtctgt gcgtcgttca tcaacacaat
     7861 cggctgcccg ttatgtggca cctgcaccac gcccggtaac aaaccgtgag ataacagttc
     7921 gcgatcggtg gtgcgtttta aaatttgccc ctgtaagcga tagcccatgc ggttactttg
     7981 cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg
     8041 atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg
     8101 cgcttccata aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa
     8161 acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg
     8221 cggaacatca ataccacccg cgaccgccag ataactgcgc atcccgtgct gcgggcgttt
     8281 aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcattatc
     8341 atccagccgc gcttcgcaac cggcacccgt cagagcaaac cacccatcag tttcgaactc
     8401 aaccgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag
     8461 caggttagca atgcgtaacg caggcatatc cagtgcgccg cagtggctga ttcccgactg
     8521 gcgaaaaccg tgacgaccgc catcctgcac agtggtatac atgcccgcac gaataatctt
     8581 cagcatactc cctccttctg cggtacaaag cgcacgctgt ctcccggacg taataagatg
     8641 ggttcgtcac gcgccggatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca
     8701 cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc
     8761 ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg
     8821 agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc
     8881 aattcaacaa cctgtttttc gctaaacccg caatgcgccg cgaccaccgc caaatccggt
     8941 ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc
     9001 tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga
     9061 ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata
     9121 tccaccagac gctgcgccag ccgccagatc cgtttctggc tagccagcgt caccggcggt
     9181 tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc
     9241 gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc
     9301 ggtattttca ttcagccact cgctcaatgc gcgaatacca ccacgttcag tggcatggtg
     9361 acctgcagca taaaaatgca acccctgctc gcgggctgaa tgaatggtct gttcagaaac
     9421 ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc
     9481 gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa
     9541 cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccagtcccg gcacaggcat
     9601 ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa
     9661 cgccgccagc tgtgcgttat tgcccagctc aggatgtacg tcaagcggca aatgccagcc
     9721 atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat
     9781 gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag
     9841 acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac
     9901 cgtctctttg ccttctacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt
     9961 cagtttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt
    10021 ctgatagcta caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg
    10081 acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca
    10141 gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa
    10201 gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgcagtcaat
    10261 tgtggaatta ttatgaataa acacgcatca caaccgcgca ctatttacta tgtcgttgcg
    10321 ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc
    10381 accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg
    10441 ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc
    10501 atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt
    10561 gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtttgtgg ctatggtctg
    10621 tttaaatcca atgtcagttg cctgctcggc gagctgtatg agccaaccga tccgcgtcgt
    10681 gatggcggtt tctcgctgat gtatgcagcg ggtaacgtgg ggtctattat cgcacctatc
    10741 gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtg
    10801 ggtatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac acatacccgc
    10861 ggcgttaata aaaaagtact gcgtgcaaca aactttctcc tgcctaactg gggatggctg
    10921 ctggttctgc tggtggcaac gcctgcgctg attaccgtac tgttctggaa agagtggtcg
    10981 gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc
    11041 aaagcagaaa accagaaaca gcgtaaggag ctggggctga ttgtgacgct caccttcttc
    11101 agtatgttgt tctgggcctt cgcacaacag ggcggcagct caattagcct ttatatcgac
    11161 cgcttcgtta accgcgatat gtttggttat accgttccga ctgctatgtt ccagtcgatt
    11221 aatgccttcg cagttatgct atgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc
    11281 gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc
    11341 gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct
    11401 ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg
    11461 gttgccatgt cgcaaattac gcgcattgaa attccgggcg tgaccggcgt attaaccggc
    11521 atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag
    11581 acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt
    11641 gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt
    11701 tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc
    11761 agcaccgatg cctgatgcga cgcttgacgc gtcttatcag
//
LOCUS       DAZPJP010000117        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPJP010000117 DAZPJP010000000
VERSION     DAZPJP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.117"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHU9J_002869"
     CDS             complement(<1..611)
                     /locus_tag="ACHU9J_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1906359.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHU9J_002870"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHU9J_002870"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1906360.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHU9J_002871"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHU9J_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1906361.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHU9J_002872"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHU9J_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1906362.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHU9J_002873"
     CDS             complement(5085..6716)
                     /locus_tag="ACHU9J_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1906363.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHU9J_002874"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHU9J_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1906364.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHU9J_002875"
     CDS             7139..7411
                     /locus_tag="ACHU9J_002875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1906365.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHU9J_002876"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHU9J_002876"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1906366.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHU9J_002877"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHU9J_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR1906367.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHU9J_002878"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHU9J_002878"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1906368.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHU9J_002879"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHU9J_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR1906369.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHU9J_002880"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHU9J_002880"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1906370.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3043 a         2858 c         2894 g         2984 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatggggtt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPJP010000118        11747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPJP010000118 DAZPJP010000000
VERSION     DAZPJP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.118"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHU9J_002881"
     CDS             complement(<1..185)
                     /locus_tag="ACHU9J_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1906371.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHU9J_002882"
     CDS             complement(230..1216)
                     /locus_tag="ACHU9J_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1906372.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTATQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="ACHU9J_002883"
     CDS             complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="ACHU9J_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1906373.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2656..3249)
                     /locus_tag="ACHU9J_002884"
     CDS             complement(2656..3249)
                     /locus_tag="ACHU9J_002884"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1906374.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3257..3547)
                     /gene="yggU"
                     /locus_tag="ACHU9J_002885"
     CDS             complement(3257..3547)
                     /gene="yggU"
                     /locus_tag="ACHU9J_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1906375.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="ACHU9J_002886"
     CDS             complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="ACHU9J_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1906376.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="ACHU9J_002887"
     CDS             complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="ACHU9J_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1906377.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            4850..5830
                     /locus_tag="ACHU9J_002888"
     CDS             4850..5830
                     /locus_tag="ACHU9J_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1906378.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="ACHU9J_002889"
     CDS             complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="ACHU9J_002889"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1906379.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6421..6984)
                     /locus_tag="ACHU9J_002890"
     CDS             complement(6421..6984)
                     /locus_tag="ACHU9J_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1906380.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="ACHU9J_002891"
     CDS             complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="ACHU9J_002891"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1906381.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="ACHU9J_002892"
     CDS             complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="ACHU9J_002892"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1906382.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(8867..9574)
                     /gene="endA"
                     /locus_tag="ACHU9J_002893"
     CDS             complement(8867..9574)
                     /gene="endA"
                     /locus_tag="ACHU9J_002893"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1906383.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9669..10166)
                     /locus_tag="ACHU9J_002894"
     CDS             complement(9669..10166)
                     /locus_tag="ACHU9J_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1906384.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(10243..11637)
                     /gene="galP"
                     /locus_tag="ACHU9J_002895"
     CDS             complement(10243..11637)
                     /gene="galP"
                     /locus_tag="ACHU9J_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1906385.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2849 a         3044 c         3004 g         2850 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcgtcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1381 gtttataatc aataaatgat gagtaaatat tcactagata tatctaataa atatcatcga
     1441 tgtttattca gtaagaaata tattttgccg aagttaatta acggggggaa aagaagtgat
     1501 taatgcgccc gcaggcgcat taatgattac tcagcgagga ataattcaag aagtgagttt
     1561 aagaataatt taccgtgctc ggttatctgc cagtaatccg cacattcggt gagataaccc
     1621 tgggcaatcg cctcgtctaa ctgtgggcga atcacatctt cgcaaagccc ggtatacgca
     1681 ataaactcca cgcgcggcgc ggcctccagc agacggaagc gattcataaa gaactcaaac
     1741 ggcttatctg cggcttcgac atcacgctgg ctttccagat accttccttg cataaaacca
     1801 cgaggatgac gcgttttagt ggtacgcaga atgcgcccat ccgggaaggt gactttgccg
     1861 tgcgcgccgc agccaatacc gatgtagtca ccaaatcgcc agtagttgag attgtgctgg
     1921 cactgataac cgggtttggc gtaagcggaa gtttcatatt gctgataacc cgctgcggtt
     1981 aataactgat gcccctgttc gaaaatatcc cacagcgcgt catcgtccgg cagcaccggt
     2041 ggtcgcgaac caaacagcgt attgggttcg atggtcagtt gataccagga aagatgcggc
     2101 ggattcagtt caatggcctg gcgtagatcg ccaagcgcct cttccagtga ttgatccggt
     2161 agtccatgca tcaaatcgag gttaaagcta cgcagcccca gcccgctcgc cagctttgcc
     2221 gcgcgtttcg cttcttgcgg gccatgaata cgcccaagtc gtttcagctt ttcttcgcta
     2281 aaactctgca caccaataga gatgcggttc acaccagcac gctgataatc gacaaagcga
     2341 tcggcttcta ccgttccagg gttcgcttcc atagtaattt ctgcatccgc tgccagcggc
     2401 aaacgcgcac gcacgccgtc cagcagcgtt tgcatcgccg ggccggaaag caggctcggc
     2461 gtaccaccgc caataaagat tgtctttact tcacggccct gagcgtaagc cacatcattg
     2521 tccagatcgc aaagcagatg ctgaacataa tcgtcgtgcg gcacttctcc tttcaacgcg
     2581 tgagagttga aatcgcagta cgggcatttc tgcacgcacc acgggatgtg aatgtagaga
     2641 ctcagcggcg gtaatttaac cattacgtaa agcgtccagc agcagtttca acgcctgacc
     2701 acggtgggaa atggcgctct tttcttcgcg ggtcagttcg gcggcggttt tcccttcgga
     2761 aggtacgaag aagattggat cataaccaaa gccaccagtg cccgctggtt cgcgagtaat
     2821 cacgcccggc cagctgccgt ggcataccag cggagtcgga tcttccgcgt gacgcagata
     2881 taccagcacg cagtggaaac gcgcctgacg ttggtcgtcc ggtacgtctt tcagtgtttc
     2941 caacagtttt tgcagattct tttgatcggt cgcgtcttca ccggaataac gcgcggagta
     3001 aatacccggc gcgccaccca gcgcatctac cgccagacca gagtcgtcgg caattgccgg
     3061 taaaccggtc acttttgccg catagcgcgc tttcagaatc gcgttttcga taaaggtcag
     3121 gccggtttct tcagcggaat ctacgccgag gtctgtttgg gccacgatat caagaccgaa
     3181 gtcgctaagc agcgacgcca gctcacgcac tttaccggca ttgccggttg cgaggacaac
     3241 tttttgcata ggatacctaa ttaattaacg ccgcgatttc tggcgggatt tgttgcggat
     3301 taatgatttt aatttgtttg tggcggccaa gttcgccttt ttcaatcacc acctggcttt
     3361 tggcaacccg gaattgctta ccgagaaact tcaccagatg actgttgccc tggccgtcaa
     3421 ccggcggcgc ggtaatggcg actttaactt cgtcgccatg taaaccgaca atagaatcac
     3481 ggctggcttt cggctgaata tagagccgta aaaccagacc gtcatcatta actgttacgg
     3541 cactcataac gccatccaca gccccggcag cagcatgttt ccggttgcct gtaatacttc
     3601 tgcgacaccc atattgatga catacagcag cagaacgagg atcatcggcg agaaatcaat
     3661 cccacccatt gccggtagca ggcggcgaat cgggcgcagc agcggatcgg ccagctgaat
     3721 cagcacgtat tcaatcggac tacgaccctg gcttacccag ctcataatcg ccatcaccag
     3781 caggacccag aaaatcagca ggccgatggt tttcagcaga atcagtaaac cagcaatcca
     3841 gatgattggc aggaaggtca ccactttaaa cagcacgatg gctttgataa aactgagaat
     3901 ataggcaacc agcagcgagg cgctgtcaat ggggcccatt gccggaataa cgcggcgcag
     3961 tggcccgata attggctgcg ttaccttcac tacaaactgt gagaaggggt tgtaaaaatc
     4021 acaatgagcc cactgcatcc agatgcgtaa taacagcacc atggtataca gctcaatgac
     4081 cgttgaaagc aggaaagtca acgtattcat ggcgttcctt taattcctta ttttttagag
     4141 taatcacgcg caccaaaaat tgcagtgccg atacgaacca tcgtgctacc tgccgcaatg
     4201 gcggcttcca tatcgtccga cattcccaga gagagcgtgt cgatatgcgg gtagcgcgtt
     4261 ttcagtccgg caaatgctac agccatttgg cgtgcaactt caaactgcct tacatattct
     4321 gactcaggcg cagggattgc catcagcccg cgcagacgta aacgcggtag ttcagcgacc
     4381 gccgctgcca gctcgtccag ttctgccagt tgaatcccgg acttactgtt ttcatcacta
     4441 atattaattt gaatcagtac gttaagaggg ggaagttctg ccgggcgctg atcgttgagg
     4501 cgggtagcaa tgcgcaaacg gtcgatggta tgacaccagt cgaaatgctc tgccaccagg
     4561 cggcttttat tagactgcaa cgggccaata aaatgccatt ctaatcctgt tacgcccagt
     4621 tcctgaaagt ggcgaatttt atctacccct tcctgaacgt agttttcacc aaattgacgc
     4681 tgcccggcat caatggcttc tgcgatggcg ctcgcaggtt ttgttttact gactgcaagc
     4741 agcgtaattt cttctggaga acggccgcaa cgcgttgcag ccgctgagat tttgtcccgg
     4801 acctgtgcca ggttatgcgc aatatcgttc attttccgag gatcttagta tgaatatgga
     4861 agaaattgtg gcccttagtg taaagcataa cgtctcggat ctacacctgt gcagcgcctg
     4921 gcccgcacga tggcgcattc gcgggctaat ggaagctgcg ccgtttgagg cgccggacgt
     4981 cgaagagcta ctgcgggagt ggctggatga cgatcagcgg gcaatattgc tggaaaatgg
     5041 ccagctggat tttgccgtgt cgctggcgga aaaccagcga ttgcgtggca gcgcatttgc
     5101 gcaacggcat ggcatttcgc tggcgttacg gctgttacct tcgcactgcc cgcagctcga
     5161 acagcttggc gcaccaacgg tattgccgga attactcaag agtgagaatg gcctgattct
     5221 ggtgacgggg gcgacgggga gcagcaaatc taccacgctg gcggcgatgg ttggctatct
     5281 caatcaacat gccgatgcgc atattctgac gctggaagat cctgttgaat atatctatgc
     5341 cagccagcga tgtttgatcc agaagcggga aattggtttg cactgtatga cgttcgcatc
     5401 gggattgcgg gccgcattgc gggaagatcc tgatgtgatt ttgctcggag agctgcgtga
     5461 cagcgagaca atccgtctgg cactgacggc ggcagaaacc gggcatttgg tgctggcaac
     5521 attacatacg cgtggtgccg cgcaggcagt tgagcgactg gtggattcat ttccggcgca
     5581 ggaaaaagat cccgtacgta atcaactggc aggtagttta cgggccgtgt tgtcacaaaa
     5641 gctggaagtg gataaacagg aaggacgcgt ggcgctgttt gaattattga ttaacactcc
     5701 cgcggtgggg aatttgattc gcgaagggaa aacccaccag ttaccgcatg ttattcaaac
     5761 cgggcagcag gtggggatga taacgtttca gcagagtttt cagcagcgag tgggggaagg
     5821 gcgtttgtga cgttgccatg cccgatgtgc aagcttatcg ggcctacaaa ggcaataaac
     5881 gtctgatgag acgtttgcgc gtcttatcag acctacaatc gctgccacat gtaggccgga
     5941 taggcgttca cgccgcatcc ggcaagatgg cggtaacgac tatccgccag gcttttaaat
     6001 cgccttaata tccctgctcg aaatagcttt cgagaataat aaccgcagag gcagagtcga
     6061 ctttgccttt gttgagcgcc cgatagccgc cctgttcaaa cagaccggaa cgggcttcca
     6121 cggtgctaag acgttcgtca tggagcttta cttcaacacc gaaacgacca tgaatacggt
     6181 tggcaaattt acgcgctctg gcagtcaatg gttgctcggt gccgtccata ttcagcggca
     6241 aaccgacgat gatttcgtcc ggctgccact ctttcagtaa acgttcgatg aggttccagt
     6301 ccggcgtacc gtcctgtgct ttaattgcag gcaaagggcg agcagtgccg gtaatgcgtt
     6361 ggccgaccgc tacgccaatg cttttggtgc cgaagtcgaa ggcgagtaag gttccactca
     6421 tcaggcgtgc cctgccacac caggcatggt gagaatatcc acaccaatca gttttgccgc
     6481 ctcgcgccag cggtcggcaa tcggcgtttt gaacagaata ttcagatccg ccggggccgt
     6541 tagccacgcg ttatcgagaa tttcttgttc cagttgacct ttttcccagg aggcataacc
     6601 tagagccacc aatacgtcag acggttgttt atcggtgccg agcgtttcca gcacatcgcg
     6661 ggaagtggtc atcactgtgt tgtctgaaat gcgaatgctg gaagcaaaat tggagggcgg
     6721 agtatgcaaa ataaacccgc gatcttcagc cagcggaccg ccgagcataa ccggtttatc
     6781 cagacggatt gattcatcac gcggctccgg cgtgatcttc agcttttcca gaatcccttc
     6841 aattttgaga ttttccagcg gcttgttgac gatgatcccc attgcaccat tggtattatg
     6901 ttcgcaaatg tagaccacgg aacgacggaa aatcggatcc tggagagcag gcatggcaat
     6961 aagaaagtga tgctgtaaat tcattgtcag aggttctgtt tcctggttca aaaaagcaac
     7021 agcgcccagt atgcgtggaa agaagggcgc tgtcactcag agtctcaacg agatccttct
     7081 cgctaaggtg ggttactgct gctgtaaacg tgcttcgatg gcatccatta acattccggt
     7141 gatcgacacc ggaaactctg cttcaatctc acgaatacag gttgggctgg tgacgttaat
     7201 ttcagtcaga cggtcgccga tgatatccag accaacaaaa atcagccctt tttctttcag
     7261 cgtcggcccg atctgacggg cgattttcca gtcactttcc gtcagcggac gaggttcacc
     7321 gcgaccaccg gcagccagat tgccacgggt ttcgcccccc tgcggaatac gcgccaggca
     7381 gtacggtacc ggctcgccat ccaccaccag cacgcgtttg tcgccatctt taatggctgg
     7441 caggtaattt tgtgccatgc agtagcgagt gccatgctca gtcagggttt cggcaatcac
     7501 gccgaggttt ggatcgcctt ctttcacgcg gaaaatcgac gcgccgccca taccgtccag
     7561 cggcttaaga atgatgtcgc tgtgtttctc ccagaacgct tttagctgcg ctttattgcg
     7621 cgtaaccagc gtttctggcg ttaagtcaga gaaccaggcg gtaaacagtt tctcgttaca
     7681 gtcgcgcagg ctctgcggct tgttaacgat cagcgtccct ttctcttcgg cacgttccag
     7741 aatataggtc gcgtagataa actcggtatc aaacggcggg tctttacgca tcaggatcac
     7801 atcgagatcg gccagcggca gatcctgttc accgacgaac gaaaaccact cttcgtagtt
     7861 ctgcttcacg ttcagcgtgc gggtatgggc gcgggcttcg ccattgatca gatacagatc
     7921 gcccatctcc atatagtgaa gttcgtaacc acgacgctgt gcttccagca acatagcgaa
     7981 actggaatct ttcttgatgt tgatgtttgc gatggggtcc atcacgatgc cgagcttgat
     8041 cattattctt ctccgttagc ccaaatcgcc aaatcgtact tgtagcgcgg taatggcggt
     8101 gagcgcagtt gtctctgtac gcaaaacgcg aggtcccaac aggatatcag taaattgata
     8161 gcgggcagtc atggcaattt catctgccga taaaccgcct tccgggccaa tcagcaggcg
     8221 gacgcgttca accggtaacg gcaacgtatt gatgctgtta ctggcgcgcg ggtgaagatt
     8281 cagtttcagt ccttcatcct gctctgcaca ccaggcttcc agatccatcg ctggacggat
     8341 ttccggcacc cggttacgac cacactgctc acaggcagca attgcaatct tctgccactg
     8401 ctggagcttc ttgttcagac gttcactatc cagtttaacg ccgcagcgct cagaaaaaag
     8461 tggcgtaatg aggcttacac cgagttcgat cgatttctgg atcgtaaatt ccattttttc
     8521 accacgcgac atcacctgac cgaggtgaat atgcagcgga gattcgcgat cgtcgatctt
     8581 gccttccagc accttcactt ccacgctttt tttgctggcg ctggtaattt cggcgtcaaa
     8641 gacctggttg ctaccgtcaa acaattgcaa cgcctgcccc ggccccatgc gcagtacgcg
     8701 cccgatatgg ttggcggcat cttcgcaaag cgcgatgtga gaatggctgg tcagtggttc
     8761 aggatgataa atgcggggga tacgcatagt taaaaatccg cgtcgtctcc ccacgcggtt
     8821 gtacgcgtgg ggtaggggtt aacaaaaaga atcccgctag tgtaggttag ctctttcgcg
     8881 cctggcaagc gcgttgcaca tacgggttat gattgccttg caccttcgcg atgcgttcat
     8941 cgcgctcgca ctcccagtcg gtaaccggat acatcttgtt ccatgcgttg aacagctgcg
     9001 tttgctggcg agagagtgtc aggttgtatt ggtcgcgcat atagaagtag gtgcgcgcaa
     9061 tggcaccgcg tgcacgcgct ggtggttcgg cagctttttc tttgaaatcg accttcatgg
     9121 cgcattgacc gtactggcct tcgccgccat tccactggct gtacataaag ttgccgcgat
     9181 cgccattcac ctcaccgact gacggctgca ggttatgcat atcgctttcc atcttgcgat
     9241 agaccggatc tttagcgcag tttttacgtc caccgtcctg ccagcactgg cgctggtgac
     9301 cgaactgcca ggcgggaacg acgtgttccc actctacgcg gctggctcgg ttttcatttt
     9361 tgcgcacctg atagccgcac gattgcagat caacaacgcc ttttttgccc tgccagttaa
     9421 ttttacatcc gcaataaaac gtaccgggcg cgtcagcgtg gacttttacc gccgcggctt
     9481 tcgcctgaga aaaactattg ataccttcgg ccaacgccgg gccggaaaat gctgcgctca
     9541 gtaccaccgc agcaatagac aaataacggt acatcactta ctccgtgtta aaacgagcca
     9601 gcaacgtagc gaaagttgtt ttggtatgca atcagccaga tcaggaaagt tcctgatagt
     9661 tcagatggtt atttcgcaac cagctgttca ccgcagtgaa cacagcgata gacggcctcg
     9721 ccacgaacta cgcgattatg gcggcgtacg gtaagctgat gctcctggca cttgcagcgg
     9781 taggggaagg tgttgcgacg cacggattgc agttcgaact gatgcgtacg acgggcggga
     9841 acacccagca cgctttccat catccacttc cactctttgc catgtggcgc tacgcggccg
     9901 aaatgtttcc ataccagcaa atgtgccagt tcgtgcggta ccacttcttc aataaaagct
     9961 tcactgtttt ccaacagcaa aacgggattg aggcggattt catagctttc cagccaggct
    10021 gttccggcgg aggttccgcg ctgggtgtaa gagagttttg gctccgggta gttacgccct
    10081 agcttcaggt tggcctgggc gagtttttcc cgcaggccgc gcataacggc ctgttggatg
    10141 gcgataggga gacgggatgt tttcatagcg gcagaggata gagcgaagaa aagatgactg
    10201 caagaggcgg cttcctccgc gacgggagga agcctgagga gattaatcgt gagcgccgat
    10261 ttcgcgcagt ttacgacctt tcatcaggtt acgttcaata tgttccagcg aaacgtgttt
    10321 ggtttccggt accagccaca atgtcagcag gataaacagt acgttcagag ccgcatacac
    10381 ccagaaggtg ttggcgttac ccagcgtgtt gagcatggtc aggaacgttg cgccaacgat
    10441 catgttggca atccagttgg tggcagtgga gcaggtgatg ccaaaatcgc ggcctttcag
    10501 cggctgaatt tcggagcaca gtacccaaat cagcggaccg gcactcatgg caaaaccgac
    10561 aataaacatc agcagcatgg cgatggcgaa atactgcgcc gacggagagt ggataccgat
    10621 atgcatcatt gtaccgagta cgcccatgcc agcagccatc accaggaagc ccagcgttag
    10681 cgttggtttt cgtccccagc ggtcaacaag gccgattgcg ataaaggtgg caagtacgtt
    10741 ggtcaggccg acaatcactg tcccccacat ttgctctgtg gtgttggtat aacccgccag
    10801 ttcgaagatt ttcggcgcgt aatacatgat gacgttcatc ccggtgaatt gctgcattac
    10861 ctgcaacagt acgccaagga acaccgcgcg gcggaagttg ctgttctctt taaacagcgc
    10921 ccagccactc tgtttaacct gcaaactttc acggatttca tccagttcgc gtttcgcttc
    10981 cgcgctggtg tcacgcaggc gtagcagcac gcgttcggca tcaacaaaac ggcgtttggc
    11041 ggcaaaccaa cgtgggctgt ctggcaggaa gaagacacca atcagcagca aaattgccgg
    11101 gatgataatc acacccagca tccagcgcca tgcaccggtg tagctgaagg cggtatcaga
    11161 aagataagca ccgaggatcc cgatagtgat catcaactga tacatcgaga tcatactgcc
    11221 acgaattttt tccggcgcaa tttcagagag gtacagcggt gcggtataag aggccacacc
    11281 caccgccagc cccagtagaa cgcgggaaag aatcagtact tcaacgtttg gcgcagccgc
    11341 agagaacagc gaaccggcaa caaacaaaat tgcgccgatc atcaggctct ttttgcgccc
    11401 gagtttaaag gagagccagc cgctgcccac cgcaccgact gccgcaccga acatcatgga
    11461 gcttacgacc cattcttgcg tgtgcgaagt aatctggaat tcatctgcaa taaacggcag
    11521 tgcgccagca attacaccga tatccaggcc aaagagtaat cccgccagag cggcaaggaa
    11581 gcagacgaaa aacgtcattg ccttgtttga ccgcccctgt tttttagcgt caggcatgat
    11641 gccctccaat atggttattt tttattgtga attaagatag gtgagtacga cgtaaaaaga
    11701 tgtgaagcaa atcacatcag tgtaatcgct tacactaaca tttatct
//
LOCUS       DAZPJP010000119        11700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPJP010000119 DAZPJP010000000
VERSION     DAZPJP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.119"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            248..1636
                     /gene="narU"
                     /locus_tag="ACHU9J_002896"
     CDS             248..1636
                     /gene="narU"
                     /locus_tag="ACHU9J_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1906386.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1718..5458
                     /gene="narZ"
                     /locus_tag="ACHU9J_002897"
     CDS             1718..5458
                     /gene="narZ"
                     /locus_tag="ACHU9J_002897"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR1906387.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKQLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5455..6999
                     /gene="narH"
                     /locus_tag="ACHU9J_002898"
     CDS             5455..6999
                     /gene="narH"
                     /locus_tag="ACHU9J_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1906388.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6999..7694
                     /gene="narW"
                     /locus_tag="ACHU9J_002899"
     CDS             6999..7694
                     /gene="narW"
                     /locus_tag="ACHU9J_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1906389.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7691..8371
                     /gene="narI"
                     /locus_tag="ACHU9J_002900"
     CDS             7691..8371
                     /gene="narI"
                     /locus_tag="ACHU9J_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1906390.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8450..9343
                     /locus_tag="ACHU9J_002901"
     CDS             8450..9343
                     /locus_tag="ACHU9J_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR1906391.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9439..10284)
                     /gene="nhoA"
                     /locus_tag="ACHU9J_002902"
     CDS             complement(9439..10284)
                     /gene="nhoA"
                     /locus_tag="ACHU9J_002902"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR1906392.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10457..11026
                     /locus_tag="ACHU9J_002903"
     CDS             10457..11026
                     /locus_tag="ACHU9J_002903"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR1906393.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11030..11257)
                     /gene="pptA"
                     /locus_tag="ACHU9J_002904"
     CDS             complement(11030..11257)
                     /gene="pptA"
                     /locus_tag="ACHU9J_002904"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR1906394.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11357..>11700)
                     /locus_tag="ACHU9J_002905"
     CDS             complement(11357..>11700)
                     /locus_tag="ACHU9J_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1906395.1"
                     /translation="FRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMGRNY
                     LASVLTGSGLS"
BASE COUNT         2733 a         3036 c         3080 g         2851 t
ORIGIN      
        1 cgatctgaag ggttgaaaca tgacatacgt tcaaagatta accccgtttg aaggttagct
       61 tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc ccccgcggtt
      121 acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta attctaataa
      181 ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg tgcatgtgag
      241 gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg actggaaacc
      301 agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa acctctggat
      361 atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg cagttaccgt
      421 taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat taaccgcatt
      481 accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc ctatattcgg
      541 tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg tctggctcgg
      601 aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg ctttgctatg
      661 cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct tctttccaaa
      721 agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag gtgtaagtgt
      781 aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc tcggcgtcaa
      841 tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat ggatttgggt
      901 accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata tcgccagttc
      961 acgcgcctca attgacgacc agctccctgt cttacaacgc ctgcatctct ggctgctgag
     1021 cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt ttgccatgct
     1081 ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg gcccatttat
     1141 cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aaattcggcg gcgtgcgggt
     1201 gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc ttaccttacc
     1261 gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc tgtttctgac
     1321 tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc gccagataac
     1381 catttatcgg gtgaagatga aaggcggtag tgatgagcaa gctcagaaag aagccatcac
     1441 cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg gcttttttat
     1501 tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg cgatgaaagt
     1561 gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg gtcggcggaa
     1621 gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc cgacaaggca
     1681 tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt tggatcgctt
     1741 tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg tgatgcatag
     1801 caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc
     1861 cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta aaaatggtct
     1921 ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc agcccaatca
     1981 tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctctaca gcgctaaccg
     2041 cctgaaatac ccgctcattc gtaaacaact gattgaactg tggcgtgaag ctctcaagca
     2101 acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa agtgcttaag
     2161 ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg aacttaacca
     2221 actgattgcc gccgctaacg tctggaccat caaaatctat ggtccggatc gcgttgccgg
     2281 tttctcgccg atcccggcga tgtcgatggt gtcatacgct gccggaacgc gttatctgtc
     2341 gctgttaggc ggcacctgtt taagcttcta cgactggtat tgcgacctgc cgcccgcctc
     2401 gccgatgacc tggggcgaac aaaccgacgt gccggaatct gccgactggt ataactccag
     2461 ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg cccacttctt
     2521 taccgaagta cgttacaaag gcaccaaaac tatcgccatt acccctgact actctgaagt
     2581 ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg ccctggcgat
     2641 ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg actactttat
     2701 caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac ctcgcgacga
     2761 tggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg gactaggcga
     2821 aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat tggtagtgcc
     2881 gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg aatccattgc
     2941 cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg ctgttgcagg
     3001 cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg taaaacacaa
     3061 tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg gcaacacctg
     3121 tccggtggtc agcgtttatg atttggtgct ggcgaattac ggcctcgatc gcgggctgga
     3181 agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag cctggggtga
     3241 gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat ttgccgatac
     3301 cgcccataaa acgcatggac gctcaatgat tatcctcggc gctggcgtca accactggta
     3361 tcacatggat atgaactacc gtgggatgat caatatgctg atcttctgcg gttgtgtcgg
     3421 gcaaagcggc ggcggctggg cgcactatgt cggtcaggaa aaactgcgcc cacaaaccgg
     3481 ctggttgccg ctggcctttg cgctcgactg gaaccgccca ccgcgccaga tgaacagtac
     3541 ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg ctcaggagtt
     3601 actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt tcaacgttcg
     3661 cgccgaacgc atgggctggc taccttctgc gccgcagctg gggcgtaacc cgctcgggat
     3721 taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc aggcgctgaa
     3781 atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc atccgcgtaa
     3841 tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg agtatatgca
     3901 gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg ccagcgacgg
     3961 gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct
     4021 ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg ttctgcccac
     4081 cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt ttattcatcc
     4141 gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa tctacaaagg
     4201 tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa ccgacgtggt
     4261 attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg aagtgctcga
     4321 ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata ttgtggcggt
     4381 ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat tgatggacaa
     4441 acttggcaat ggtggtaaag ggatttcgtg gaatacccag gatgaaatcg atttcctcgg
     4501 caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga ttgacaccgc
     4561 cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc atgttgcagt
     4621 caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc tggcgctgca
     4681 caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta aaattatctc
     4741 cagccccaca tggtccggtc tggaaagcga tcacgtctcc tataatgcgg gatacaccaa
     4801 cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct atcaggatca
     4861 tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta tcgacacccg
     4921 tagcgtcagt gagatgcgcc agatcccgcc aaacggcttc ccggaaaaag cacttaactt
     4981 cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc tgctaatgct
     5041 gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc gagaactgac
     5101 cattgtcgat aacgactggg tggaagtctt taacgccaac ggcgcgctga cggctcgtgc
     5161 ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgccc aggaacgcat
     5221 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     5281 ccgtgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
     5341 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     5401 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     5461 atacgttcgc aagtcggcat ggtgcttaac ctcgacaaat gtatcggttg ccatacctgt
     5521 tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     5581 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     5641 caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg cagcaagatg
     5701 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga ttactacgaa
     5761 cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca tatccctact
     5821 gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg ggggccaaac
     5881 tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa cttcgaggcc
     5941 atgcaaaagg agatgtacgg gcagtttaaa aacaccttca tgatgtacct gccgcgcctg
     6001 tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc tatctacaaa
     6061 cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg gcgtttgtgc
     6121 attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa
     6181 tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc agaaacctgc
     6241 gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat tgaggaagcg
     6301 gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtgtt cctcgatcca
     6361 cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa cgtgattgaa
     6421 gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc gctaccgctg
     6481 caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc gccgattcag
     6541 tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc
     6601 ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac cggtccggta
     6661 ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca aaccgtggaa
     6721 ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca ggtcgaagaa
     6781 atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc gaccagccat
     6841 cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac ctttggcgac
     6901 ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat cgatgccatc
     6961 aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc aaagtgatcg
     7021 gcctgttgat ggagtatccg gatgagctgt tgtgggaatg caaggaggac gcgctggcgt
     7081 tgatccgccg cgacgcgccg atgcttacgg atttcaccca caacctgctt aacgcgccgc
     7141 tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc acgtcgctgc
     7201 tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg gtggacctgc
     7261 tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc gattatctac
     7321 cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa ggattgctca
     7381 atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag gcaccgtggt
     7441 acgcgttgtt tgatgctctg ctgcaactgg cgggaagtat actttcaagt gacagtgtca
     7501 caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat gcagtgtggg
     7561 aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc tcgccgctta
     7621 atcaatatca gcgacgcttt agccaggatg tcgcgccgca gtatgtcgac atcagtgcgg
     7681 gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc cgtacatttg
     7741 tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt acacctggcg
     7801 cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga atctgttcca
     7861 tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc cgcactggat
     7921 gtacgcgtgg tttttgccag tggcagcgaa acaactcatg gcgatggtgc tcggcggtat
     7981 ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcagtc tgacaaatca
     8041 gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt tgctgatcca
     8101 gtgtctgttg gggctaagta ccataccgtt ttcagcacag tatcctgatg gtagcgaaat
     8161 gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat cgtcagagat
     8221 gctcaacggc gtagcgttcg tcttccgcct gcatctggtg ttgggaatga ccatcttcct
     8281 gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg ccgtttgagt actttactcg
     8341 tcgatatcaa attgtgcgtt cgcggcgata atttcaccct ggccggatag cccttcgctc
     8401 tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa tgaaaccgca
     8461 ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg ccggtgttgt
     8521 tttccccgcc gataatctta gcgaagcaca aatgcagctt atcgcccgcg agttaggcca
     8581 ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc gctactttac
     8641 gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact atgtacgtgc
     8701 gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag gaaaacatcg
     8761 cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag gtacgccggg
     8821 ctttgaactg ccgctggagg gtgaagtacg cgcggcgatt atcaacgcat tgcatcttac
     8881 tgaagacgat attctgccgg gtttgccgat ccaggtcgcg acgaccgggc attctaaagt
     8941 gatgatccca ttgaagccgg aagtggatat cgacgctctt tcgcctgacc ttaatgcgct
     9001 gaccgctatc agtaaacaga ttggctgcaa tggtttcttc ccgttccaga tccgtccagg
     9061 caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg aagatccggt
     9121 gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg tattgcccca
     9181 cgatggcaac gtgttgtgtg ttaaaggcca tcaggggcgc gcattggggc gtgacggcat
     9241 gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca tttcaggcac
     9301 agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc tgaaatcgtt gcctgatgcg
     9361 cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt tacgccgcat
     9421 ccggcaacct gacataaatt attttcccgc ctccggatgc gtatcaaacg ccgccatcac
     9481 cagcgctaac tcatccacag taaagccatg tttcgcatca tccacgccca gaccaaactg
     9541 ttcctgcatc acggcatata acgacgccac atccggtaga tttcgctgct ccaccgcgtg
     9601 cccattttca taatgggtaa aatgaaaatt ggtcagtgtc agcttgccgc cgtccggcaa
     9661 atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg ccgaccagaa
     9721 attgcccatc acataatcgc tttgttgctg ctcgcagaga tcgaagcggt acatcggctg
     9781 ccaatgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca acaaccgata
     9841 ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg cggttagcgt
     9901 ctgcccaccg aaaccgacat cagcaatcca tttttcccct tccagttcca ccaacagcaa
     9961 acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc ctaacaagct
    10021 gcgaacgtta aaccccagct cgcgtaacac ccgctcaaac acgccattct gctcaaaaca
    10081 gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat caagctgtat
    10141 ttccctcggc agcaaaacgt caaggttttc aaacggaatg gtgcaattgt gtttcaggtg
    10201 caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa tacgggcaaa
    10261 atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc aacttataac
    10321 ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca cgtttgcaaa
    10381 tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa aaaaaccttc
    10441 ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg ccagccgtct
    10501 gttaaatcat ggcccaactg tcttgatcac cagtttcgat gagcaatccc agcggcgtaa
    10561 cattatggct gcagcctggt caatgccggt ggagtttgaa cctccacgcg tggcaattgt
    10621 ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaagt ttggcatcgt
    10681 tatcccgggc gttgcagcaa ctaactggac gtgggcggtg ggaagtgtgt cggtgcgtga
    10741 agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtgc ttggtttgcc
    10801 acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg cgacttctgc
    10861 gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca gcagcggcag acgcacgggt
    10921 atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc atcatttagg
    10981 tgctggcacg tttgttacta gcggcaagcg tgttacggca ggttaagcat tacatgctgt
    11041 aaccgggttt ctttatcaaa gcctccattt ggggctcgat ttcggcatcc cagatagctt
    11101 gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct ttactgttca
    11161 gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttgt tgttcgtcca
    11221 gttcacgcgg aaagcattta atgtcgatgt gcggcatgat tacgctcctt ttgaaagatg
    11281 tcagtaaaat taacacacat tgtgaatgtc atgtgtccgg taaagcgttt atacgacaat
    11341 aatgcccagg gcaagattac gaaagcccgc tccccgtaag gactgacgcc aggtagtttc
    11401 tgcccatggc tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc ttatcattcg
    11461 tcaaaatatt aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca tttcctcttc
    11521 ttattttgca gtcgtcttca ttcattacca cgtccagacg ccaatgcagc ttattctcca
    11581 cgtgccagtg gtttcggatt gctgtggcga acttctctgc ggttaaatca gcagaactga
    11641 tataatatct gaccgtcatt tctggctctt tcttttgttc tgctattatg gaccgaaagg
//
LOCUS       DAZPJP010000120        11533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPJP010000120 DAZPJP010000000
VERSION     DAZPJP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.120"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..445
                     /locus_tag="ACHU9J_002906"
     CDS             <1..445
                     /locus_tag="ACHU9J_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFR1906396.1"
                     /translation="CFSPLLFRCVLLIVRSVSEYKFTFFAKKKKGSKESSIHLSPYAD
                     TRCPESMRKHWDVLLSLISKAINNDEIIVMHSHLFTPARINKLIRRLESYGLNFSVIT
                     YPRKTSLYERLSIPFVYLLFQWSLPHLHSDGMTVVIRPLQPPHTN"
     gene            479..1546
                     /gene="pilL"
                     /locus_tag="ACHU9J_002907"
     CDS             479..1546
                     /gene="pilL"
                     /locus_tag="ACHU9J_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFR1906397.1"
                     /translation="MKKKCLTLILPSLLSGCAGISGGNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKVTPPPRVSVPETVKTAQPVSESRPLPSPTINQNFSRLIITGKP
                     GSVPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTRLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSPSTPAAIPTPTGSTVKSVPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1546..1983
                     /gene="pilM"
                     /locus_tag="ACHU9J_002908"
     CDS             1546..1983
                     /gene="pilM"
                     /locus_tag="ACHU9J_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR1906398.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQQMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVEAL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1997..3679
                     /locus_tag="ACHU9J_002909"
     CDS             1997..3679
                     /locus_tag="ACHU9J_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR1906399.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREQKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTT
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3672..4967
                     /gene="pilO2"
                     /locus_tag="ACHU9J_002910"
     CDS             3672..4967
                     /gene="pilO2"
                     /locus_tag="ACHU9J_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR1906400.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTKPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDVDVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVGPTA
                     EEIAARARLQFKKPEPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4954..5406
                     /gene="pilP"
                     /locus_tag="ACHU9J_002911"
     CDS             4954..5406
                     /gene="pilP"
                     /locus_tag="ACHU9J_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR1906401.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVNLSSETVSGFSGVTTSLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5417..6970
                     /locus_tag="ACHU9J_002912"
     CDS             5417..6970
                     /locus_tag="ACHU9J_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR1906402.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDITPKVVSLSELQEAGQDDENKGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     VMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEEAVKLAWSRAISSAMRLDPDAIMEGEMRDLVSMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNPHGCSDCNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMHGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDA"
     gene            6983..8068
                     /locus_tag="ACHU9J_002913"
     CDS             6983..8068
                     /locus_tag="ACHU9J_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR1906403.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGQKWHPFAELATDCIESLRENSGENSLEYTLGLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKKQLHQAIWQMAIYPVGLLIMMTGTLYVLNAELIPVLSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVVTGLTSWSLKNWKNRSFADNIMPWS
                     IYKDIQGATFLLNMAALLKAKMTTLNSLNVLQEFASPWLSTRLDSIIYRVRQGDHLGL
                     ALRQCGYHFPSRESANFLSLLQGDGATELIGNYGQRWLVQTLERVKKRAAVVRLIMLI
                     FLVMSLLLLVMAVVDIQSIGDNSMGSI"
     gene            8085..8699
                     /locus_tag="ACHU9J_002914"
     CDS             8085..8699
                     /locus_tag="ACHU9J_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR1906404.1"
                     /translation="MLIQNINVNVLKKNEKETSHDKGWGIMEQGAIALVVIIVLALVF
                     GGLYMLRSRTSVANESSNIQTIITSTQGLLKGSDGYTFTSGAKMTGALIQMGGVPKTM
                     TVRGTPSSGTATLYNSWGGGVTVAPASTSGFNNGFTVTYEKVPQDACIQIATQISRTG
                     LTNGITLNSTAHSDGKVTTEEASAQCTADNGSTGTNKLIFTING"
     gene            8709..9269
                     /locus_tag="ACHU9J_002915"
     CDS             8709..9269
                     /locus_tag="ACHU9J_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1906405.1"
                     /translation="MSGGKVPLRLISLILLCSLIYPARASYIAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLKPGAININKDRKTGKASSTDYGLMQINSTHIPKLIK
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKVWRIYREMKGICLPGQGGRQCRQS"
     gene            9254..9910
                     /locus_tag="ACHU9J_002916"
     CDS             9254..9910
                     /locus_tag="ACHU9J_002916"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFR1906406.1"
                     /translation="MQTIVGISLSPVYILPLMFIFSIIGRTLLFRVRYFLSDTEHLWY
                     KTHTAVLSGIWLYSIAVALIILSPSQLLYRIHAVLILSFMLQMAVTDALTGLLPGTFT
                     RRFLIAGILSQITTDIWWLRATEIVSAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWSVLLGAGGFVLWHSTWCIKGHKEGPLGPWLCLGHVLLLLDNLYQPLWVI
                     "
     gene            9910..11202
                     /locus_tag="ACHU9J_002917"
     CDS             9910..11202
                     /locus_tag="ACHU9J_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR1906407.1"
                     /translation="MKKYDRGWASLEIGAALLIVMIIVAWGAGIWQDYLKTKGWQAEA
                     RLVSNWASAARSYIGKNYTTLQASSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNTQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGAILSCQSGTWGTIGGKLKVTQLSTTGYLGQFDFCAIARM
                     GNAEDAHYCQVVESPAGSRKWYKYEHKTGCIASCVTLN"
     gene            complement(11199..>11533)
                     /locus_tag="ACHU9J_002918"
     CDS             complement(11199..>11533)
                     /locus_tag="ACHU9J_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFR1906408.1"
                     /translation="TSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAGGA
                     CANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGAGV
                     FSVFGYQT"
BASE COUNT         3283 a         2740 c         2653 g         2857 t
ORIGIN      
        1 ttgcttttcc ccgctacttt tcagatgtgt acttcttatt gtaaggtctg tatctgaata
       61 taaattcaca ttctttgcga agaagaaaaa gggcagtaag gaatcaagta tacatcttag
      121 cccctatgct gacacacgtt gcccggaatc aatgaggaaa cactgggatg tattactttc
      181 tctgatatca aaagcaatta ataatgatga aatcattgtt atgcattcac atctatttac
      241 tccggcaaga ataaacaaac ttataaggag gctggaatct tatggtttaa atttctccgt
      301 tattacatac ccacggaaaa caagtctgta tgaaagatta tcaatccctt ttgtatatct
      361 cttatttcag tggtctctcc cgcatttgca ttcggacgga atgacagtcg tcattcgccc
      421 tctccagccg cctcacacca attaacaaac acgtatatac gaacacggag tctccgttat
      481 gaaaaaaaaa tgtctgacac ttatactgcc gtcattattg tccggctgtg ccggaatttc
      541 cggcggcaat aacgtggcac catcagatac gccagctctg gtctttgttg acggacaaat
      601 cagtgagagt gccggcgttc tggcacttac tcaacaacgt ctttctccac ctaaagtaac
      661 tccgcccccg agagtcagcg tgccagagac agtaaaaaca gcacagccag tcagcgaatc
      721 ccgaccgtta ccttcaccaa caataaatca gaatttctcc cggttaatta ttacaggcaa
      781 gcctggtagc gtccctgtaa cactatcacc gacggcccgt aaccggaccg tcagtcagtg
      841 gataaaaaca ttactaccgg aaggatggaa attcagacaa gagaatgcag ccacgcccaa
      901 actgaatacc agactggttt catggtctgc caatgatcag tggacccgtt ctctgaaccg
      961 gttgctggaa gaacaacacc tctggggcca tctggactgg gataataaaa cgttgaccgt
     1021 cacaacatcg gctgctgcac cggcacagga cacggcaacc actatctcgc cagcagccat
     1081 ggtaaccaca tccacttccc cggagtctcc aacgacagca aatagccaga acaaaccacg
     1141 aaacccattc aggggaaaca gcgtatctcc gtctacgccc gcagcaatcc cgactccgac
     1201 cggctctaca gtcaagtctg tcccactcat gaccgggaca cctgtcaaac cggtatctca
     1261 gggtaaagag tggcgtgcgc cggcaggcac tacattaaga gagaacatta ttaagtgggc
     1321 agaagaaaca aaatgcgaaa gcatggcatc aacgcactgg atggtcatat ggccactttc
     1381 tgtaaccgat taccgtctgg atgcgccttt gatgttccgg ggatcatttg aatcagccat
     1441 ggagcaggta tttgagttat atcggaccgc gcaaaaacct ttatacgcac aggcctcccg
     1501 catgcagtgc atcattacag tttctgatac aagggatggt cgctaatggg atggctggtc
     1561 atggccgtcg gcctgaccat tctgattatc actggaagct accagaatca gcagatgtct
     1621 gaaaccacaa acgcccagca atatgcaagt gcgtctgtat gggcaagtca gatactaatg
     1681 attgctaaca gaatcaatga tatcagatac gtatcaggcc agcaggacgg agtaatttcc
     1741 tcagataaac tggctctccc tgtcactccg gactcccgaa ttaaacatca gttacaacag
     1801 gggcgtctgt gggtatggat gcctgaacaa ccaggacttg ttgaagcttt gcgttcaaaa
     1861 agtcgtggtt ctgcactaat agggatcttc caaaacgggc aactcacctg gctatcagga
     1921 actgcaaccg gtctgacacc cccggcaggc attactgctg gttctgtcgt ctacgttaac
     1981 taatctggat atattcatga aaaaatcaca ccagcgttca atgaagctgg cggtgctccc
     2041 ctgcatgatc gccgtcgctc tttccatctc cggatgtact ttcagcgaaa tcaataaaat
     2101 gcagaaaaaa gcacaggaag actcagcaca tgcacgggaa aaggtatcag ccctttctgc
     2161 ccgtaaatca caggctctta cctggctcga taatcaatgg ataaaccctg ttccggtcgc
     2221 tcaggtatca agagagcaaa aacaaacagc tccggcctgc tacatcacgc aggcaagaaa
     2281 aggagagatc actctgcagg aactggggca acgtattact gccgtatgcg gcatccccgt
     2341 aatcatcacg ccggacgcag ccaattcaac tcttgaagga ggcgctaccc gccagatgac
     2401 aggaacactc cctgcacctg atgaaaatgg acgtctgccg ttaagcagcc tgggcagcac
     2461 aacaatgact accaccactc agccattaac gttgaataac ctcatgtggc agggagatat
     2521 caatggtctt ctggatctga tggccagccg gagcggtctg tactggcgca tggataatgg
     2581 tcggattgta ttctatctga ctgaaaccag gacatatcca ctgcatatgc tgaacaccaa
     2641 aaccagcagc agttccagtg tcagctctgg ctcaacaagc acaatggggg caacaggagg
     2701 tcaggataac tcagcatccg gtgatgctac gtcctctcag agcacaaccg tcggtcagga
     2761 atacgatctg tatgaagaca tccggaaaac tattgaagca atgctgacac cagaaaaagg
     2821 ccgttactgg ttatctgcat cgagctcaac gctgacagtc actgatactc ctgctgtcca
     2881 ggaagccgtc gcacgatatg tggacgaaca aaacagtatt atgaaccgcc aggtagctct
     2941 gaacgtacag gtcctgagtg tcagcaatac cagaaacgag cagttcggtc tggactggaa
     3001 ccttgtttac aaatcgctac attccgccgg agcaacgttg aacaatgcaa gcggagattt
     3061 tacaggcgct acatctgcag gcgtatcaat tctggatacg gcaacaggga atgccgccaa
     3121 attcagcggt tccagtcttc tgattaaagc actgagtgaa cagggcgatg tcagtgttgt
     3181 gacttcacaa gaaagcactg tcacaaacct gacgccggta cctatccaga tggcagatca
     3241 gacagtttac gtcgcccagt cagcaacaac tacgactacg gatgtaggag caacaacaac
     3301 attaacaccg ggcatgatca ccaccggatt caatatgacc ctgctgcctt taattcagaa
     3361 aacgggcaat ctccagttac agatgaactt taatctgtca gatcccccaa ctatccgtag
     3421 ctttacgtca aaagacggaa acagttacat cgaaatgccg tacaccaaac tgcgttcact
     3481 gagccagaag gtcaatctga aagaaggaca atcgcttgtc gttactggat tcgatcagaa
     3541 caatacgacg acaagtaaag ccggtacatt tacgccagca aatccattat ttggcggttc
     3601 acaaaccggg aaaaatgaac gcagcacgct tgtgatcatc attaccccga ctttcccgtc
     3661 aggaggcaat aatggctgat gaagatatca acccggttgt gcttctcgca gatcctaaag
     3721 taaatcatcg cgtgtgggct gcctgtctta aatggactcc tgtcgtaaaa aaacagcgag
     3781 tcccctcaca tcataaacac aagtctcatg taaaacccag acggcttacc tccctgaaag
     3841 tgactgtagg cagcagaaat tcccgtggaa agatcagccg ccttaccggc acaggaatac
     3901 tgaccaagcc agaacgcaat cattattttt ccctggcact ggctttctgc tcatgggtga
     3961 gaaatggata cggtgtcttc cggtacagcg ataaggagct cttattcctt gcttccatta
     4021 atggccagcc tgccgttatg gctgacttat caggaaatga cgtcgacgtt gcccaaaagg
     4081 tcagcctttt tctggcgatg aacgaagagc cccccgaaaa atggcaggtt gtatcgtctc
     4141 tggaacatcc tgataactgg gaaagtatta ttacccggtt atcatcagca gacctccgcc
     4201 gttgcaaact tactgtcggc aatcgcagca aattcacact gcctgccgta ctgtttttag
     4261 tagcagccgg cgcaggcaca gtattctgga tgactcagcc agagcctgat gtcggaccga
     4321 ccgccgagga gatagctgca cgggcaagat tacagtttaa aaagcccgaa ccaccaccgg
     4381 agctcccgca tccatgggcg tcacaacctg taatcagtga ttttctgaaa gcctgcgcgg
     4441 accttcgaaa accatcaccg gtcgcgctag aaggctggaa acttaccggt ggaacatgta
     4501 ctcccgagac attcacactg atttacgaac gacagccagg aggaactatt gaaggctttc
     4561 tggcaagaag caaagaaatc tttaatgtta ttcctgactt taaccttaaa gatggtgcac
     4621 gcctggcttc tgtaacccgg ccactgcctt ccctgccccg cagggatgag gcagtccctg
     4681 ccccctcaga acaactgatg cgggtgttca cgtggtttca gaaaaaacag ttaacgccgg
     4741 ccattaatga aatagccata ccggaaccat taccaggtaa cgatggtgaa ccagcccctg
     4801 ttcagaaatg gaaagaatat cagttcagtt tatcaacgcc tgtaaatcct gatgagttgt
     4861 tccctttatt tcaggatacc ggcgttcgtc tgagcaatat ccattttgaa ttaaacggcg
     4921 gtactttcag ttattcctca gaaggtcata tatatgcgtc caggtaaatt actcattatt
     4981 ccatccatat ttttcttctc tggtttttca ttcgcaacaa cacaacctct tgtcacaata
     5041 ggagaacttg aagcacaaca aaaccggaat attcttttac aggcaaaagt tcagggcgca
     5101 cagctacaga aacaactcga agaatcagat gttaatctgt catctgaaac tgtttcaggg
     5161 ttctctggtg tgactacctc cttgccatca gtttcagagc agccaacatc taaaaacaga
     5221 aaggaattgc ccgttattat ggaaataaac ggcaaagata aacgtcttca tgccgttctt
     5281 cgcatggcag atggacgtca gacaagcgtc accactggta gccagcttcc gggtacatcc
     5341 gtcacggtga agtcgatctc attgtctggc gtcacgttaa gtgatggcac cacccttact
     5401 ttctgagtga tgtgatatgg acaaatataa tctgaaggat gctctccttt ttatatcccg
     5461 gggagacacc catgaaattc tcatcgaaac aaatcagcga acaagacctg acgtacagag
     5521 taatctccag gaactgctaa agttataccc tgacatcacc ccaaaagtag tctcgctgtc
     5581 tgagttacag gaagccggtc aggatgatga aaataaaggt ccgaaagaac gaattattca
     5641 tctgaaagat cttgcagatg tcagtgaaag tgagaagaaa gttctctcct attttgaaac
     5701 ggccagaaaa cttggtgctt cagatattca ttttttaatt tcagagtcta ttttcaaagt
     5761 tcgcatgaga atattcggtg aactgcagac tgtagatgaa gatcaacctg cgttaggtta
     5821 ttcactttgc gcaacagcca ttctgtcaat ggctgatgtg actgaaacca gtttcttccc
     5881 tcaaagggaa caggatgcgc gcctgtcccc acaattaatg cgaaaaatag gaatatttgg
     5941 tgcacgatac agtcacagac ctacgggaga tggtctgatt gcagttatgc gcctgatacc
     6001 cgatgacgga gacaaagttc ccacgtttaa gcaactgggc tttatcccgg agcaaatccg
     6061 gttgctgaac gtcatgctgc gccgcccgga agggaaaata gttctgtctg gcccaacagg
     6121 ttcgggtaaa tcaacaacgt tacgcagtgc ctgccgtgta tatcttgacg ataatcaggg
     6181 gcgacacctg ctgacgatcg aagatccgct ggaaggacag atactggggg caacacaaac
     6241 cccgattatc tgtgacaaat ccgacgaaga ggccgtcaaa ctggcatgga gtcgtgctat
     6301 ttcatcggca atgcgacttg atcccgatgc catcatggaa ggggaaatgc gcgatcttgt
     6361 ctcgatgatg tcaaccacct acgcagctca gaccggtcac atcgttctga cgacattaca
     6421 tacaaactca gctctgggta taccggagcg aatgattacg atgggtatga atgccgatct
     6481 tatttgtgat gcgcaattac tgattggaat gataagccag cgccttgtac caacgctgtg
     6541 cccatcctgc cgtattccat gggaaaagcg agcacctgaa cttagcgacg acgagcgtga
     6601 ttaccttgag cggcactgca ataaagattc tctctgcagt acagataata tctggttccg
     6661 aaatcctcac ggatgctccg actgtaatca cgacgtaatt attaacggca gaaagcgtgg
     6721 tgaaataggt aaaggtctca ccggcaggac cgtaattgct gaggtgatag aacctgataa
     6781 ccgcttattt cagattctga aaacacgcgg caaagttgca gcacgtaaat actggcttga
     6841 aaacatgcat ggcatcagcc gtgtggagca tctgcttcgc cgtatcaatg aaggcctggt
     6901 ggaccctctt gaagccgaca gaatcatccc tctggacgaa gacgaacgcc tcagcatcga
     6961 tgatgcctaa caggagaaca acatgcgtga aatgaatttc agtcaacggc tgaggcgttt
     7021 cattgtcaga aaaacgttca gcgcccccta cagggtgcag ttttacgaag ctctgcgttt
     7081 tctgctggaa aataaacagc cattaaagac cgcactggaa cagatgcggg atgcctggac
     7141 agactttgga caaaaatggc acccctttgc tgaactggcc acagactgta ttgagtctct
     7201 tcgtgaaaac agtggcgaaa actctctgga atatactctt ggtttatggg ttccccagga
     7261 agaggctgca gtaatcagtg ccggaataag aagtggttca attgtggatg cactgcaatt
     7321 tgccacaact cttacagatg ccaaaaagca actccatcag gccatatggc aaatggctat
     7381 ttatccggtg ggattgttaa tcatgatgac ggggacatta tatgttctta atgcagaact
     7441 aatacctgtg ctgagcaaaa taagctctcc tgattcatgg agtggtgctc ttggttttct
     7501 ctatggatta tctgtgtttg tcgataatta cggcgctatt tgtgccgttc tttttgcagt
     7561 ggtcacaggg ttaacatcat ggtctctgaa aaactggaaa aaccgctctt ttgccgacaa
     7621 tataatgcca tggagtattt ataaggatat acagggggca acatttctcc tgaatatggc
     7681 tgcacttctg aaagccaaaa tgacaacact caattcactg aatgttcttc aggaatttgc
     7741 atcaccatgg ttaagtacac gccttgacag cattatttac cgcgtccgcc agggggatca
     7801 ccttgggctg gctttacgac agtgcggtta tcatttccct tccagggagt ctgccaactt
     7861 tctgtcttta ttacagggag atggtgcaac ggagctaatc ggcaattatg gccaacggtg
     7921 gcttgtacaa acgctggagc gtgtaaaaaa acgagcagct gttgtgcgtt taattatgct
     7981 gatatttctt gtcatgtcac tcctgttact ggtcatggcc gttgtagaca ttcagtctat
     8041 tggcgacaac agtatgggta gtatttaaat aaaacgaggt gtttatgtta attcaaaata
     8101 ttaatgttaa cgttctgaag aaaaatgaga aagaaacgtc tcatgacaaa ggctggggga
     8161 ttatggaaca aggtgcaata gctcttgttg taattattgt tcttgccctt gtttttggcg
     8221 gattgtatat gttgcgttca cgaaccagtg tggcaaatga aagttcgaat atacagacaa
     8281 tcatcacaag tactcagggg ctgctaaaag gtagcgatgg atatacgttt accagtggag
     8341 ccaaaatgac gggtgctctc attcagatgg gtggcgtacc caagactatg acagtgcgtg
     8401 gtactccatc atcaggtaca gcaacgctct ataactcatg gggcggtggc gtaactgttg
     8461 ctccggcttc aacatcaggt ttcaacaatg gttttaccgt cacctatgaa aaagtcccac
     8521 aggatgcctg tatccagatt gccacacaaa tcagtcgtac tggtttaact aacggtatta
     8581 ccctgaacag tacggctcac agtgatggaa aagtcactac tgaagaagcc agtgcgcaat
     8641 gcacagctga taatggcagc accggtacaa acaaactaat cttcaccatc aatggctaag
     8701 gagttttaat gtccgggggt aaagtgcccc tcagacttat ttcattaatc ctgctttgtt
     8761 ctcttattta tcccgcccga gcttcataca tcgcccccaa acaaatctgg actaacaagt
     8821 gggacaattg ctttgccgcc gccggcgcac gctatcaaat tgaaccatta ttgttgaagg
     8881 ccatttctgc cggggaatct tcattgaagc cgggcgcaat taacataaat aaggacagaa
     8941 aaacaggcaa agccagtagt acggattatg ggttgatgca aataaactca acccatattc
     9001 caaaacttat aaagatgggc gtaatcaaaa agtctgagga tttaattacc aaaccatgtc
     9061 ttaatatcca tataggaagc tggatactgg caagacattt tcagatatgt ggcgtcagct
     9121 ggaattgcct tgggtcatac aatgccggat tccgtaagga cagacatgaa acaagagagc
     9181 aatatgctaa taaagtatgg agaatttatc gtgagatgaa agggatatgc cttcccgggc
     9241 agggaggcag acaatgcaga caatcgtagg cataagtctc tcccctgttt atatcctccc
     9301 attgatgttc atattttcca ttattggacg aactctgtta ttcagagttc gttatttttt
     9361 atctgataca gagcatctct ggtataagac acacactgca gttctgtcag gaatatggct
     9421 atatagtata gctgtggcat taattatttt atcgccatcc caacttctct accgtattca
     9481 tgctgttctt attctttcgt tcatgctgca aatggcagta acggatgcac taacaggcct
     9541 cttacctggt acattcacca ggcgtttttt aatcgctgga atattatcac aaatcacaac
     9601 tgatatatgg tggctccgtg caacagaaat tgtctctgcc gcaattgtct tattctgtct
     9661 gcataaactg gttaaccgcc atcgtcttaa tattggcaca ggagacctgt ggcttattgc
     9721 aggtattacc gcatggtcag ggctatataa cgccatatgg agtgtgttgc tgggcgcagg
     9781 aggatttgtc ttatggcatt caacatggtg cataaaagga cataaagaag gcccactggg
     9841 tccatggctg tgtcttggcc atgtcctttt gctactggat aatctttatc aaccactatg
     9901 ggtaatctga tgaaaaaata tgaccgaggc tgggcatcgc tggaaattgg cgcggcttta
     9961 ttgattgtta tgatcatagt tgcctggggt gcaggcatat ggcaggacta tttaaaaacc
    10021 aaaggttggc aggctgaagc tcgtcttgtc agcaactggg ccagtgcagc ccgctcttat
    10081 atagggaaaa actacacaac tctccaggcc agtagtacca caacaacccc tgccgttatc
    10141 acgacaacca tgctgaaaaa taccggcttt ttatccagcg gctttactga gacaaacagc
    10201 gaggggcagc ggttacaggc atatgtagtt cgaaacaccc aaaacccgga attactacag
    10261 gcaatggttg tatccagtgg tggcacccct tatccagtga aagcacttat ccagatggct
    10321 aaggatatta ccactggtct tggtggatat atccaggacg gcaaaacagc cacaggtgca
    10381 ttacgttcct ggtcagtagc tttaagtaat tatggtgcca aaagcggtaa cgggcatatt
    10441 gccgtactgt tatcgacaga tgaacttagt ggtgcagcag aggacactga tcgtctttac
    10501 agattccagg tcaatggtcg ccctgactta aacaaaatgc acacggccat tgatatggga
    10561 tcaaataacc tgaataacgt tggggcagta aatgcccaga ctggtaattt cagcggcaat
    10621 gtgaatggtg taaatggcac tttcagcggt caggtaaaag gcaatagcgg aaactttgac
    10681 gtaaatgtca ccgctggcgg tgatatcaga agtaataatg gttggttaat tactcgtaac
    10741 agcaaaggct ggctcaatga aactcacggt ggcggatttt atatgtccga tggatcatgg
    10801 gttcgaagtg taaacaacaa gggcatctat accggcggtc aggtgaaagg cggtactgtt
    10861 cgtgctgatg gtcgccttta tactggtgaa tacttacaac tggaaagaac tgccgttgct
    10921 ggcgcatcat gttcgcctaa cggccttgta ggccgcgata atacaggggc aatactttcg
    10981 tgccaatccg gtacgtgggg gacaataggt ggaaaactca aagttactca gctttccacc
    11041 acaggttatc tggggcaatt cgacttctgt gccattgcca gaatgggcaa cgcagaggat
    11101 gcccactact gccaggtagt tgagagccca gcaggttcac gcaaatggta caaatacgag
    11161 cataagacag gttgtattgc gtcgtgtgta acgctcaatt aagtttgata tccaaatact
    11221 gaaaataccc cggcaccaca tgatgtattt tctgttggat aggatgttat ctgataggaa
    11281 gtacctgcag gtacagcaaa gctgataaag gctgtttttc catatgcagg gttattgctg
    11341 gcattcacgc taattagcgt cccaccaaca tatccctgca gtcgggatgt atttgcacat
    11401 gcacctccgg cagatcctcc attacctcca gatgcataaa taaacaatgt actgccccct
    11461 gaattccgcc ctgagaatga acctctatgt gaacctaagt ttgtgtaaga accattgagc
    11521 gaaccagaag tct
//
LOCUS       DAZPJP010000121        11449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPJP010000121 DAZPJP010000000
VERSION     DAZPJP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.121"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            551..2119
                     /gene="cydA"
                     /locus_tag="ACHU9J_002919"
     CDS             551..2119
                     /gene="cydA"
                     /locus_tag="ACHU9J_002919"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1906409.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2135..3274
                     /gene="cydB"
                     /locus_tag="ACHU9J_002920"
     CDS             2135..3274
                     /gene="cydB"
                     /locus_tag="ACHU9J_002920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1906410.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3289..3402
                     /gene="cydX"
                     /locus_tag="ACHU9J_002921"
     CDS             3289..3402
                     /gene="cydX"
                     /locus_tag="ACHU9J_002921"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1906411.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3402..3695
                     /gene="ybgE"
                     /locus_tag="ACHU9J_002922"
     CDS             3402..3695
                     /gene="ybgE"
                     /locus_tag="ACHU9J_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1906412.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3845..4249
                     /gene="ybgC"
                     /locus_tag="ACHU9J_002923"
     CDS             3845..4249
                     /gene="ybgC"
                     /locus_tag="ACHU9J_002923"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1906413.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4246..4938
                     /gene="tolQ"
                     /locus_tag="ACHU9J_002924"
     CDS             4246..4938
                     /gene="tolQ"
                     /locus_tag="ACHU9J_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1906414.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4942..5370
                     /gene="tolR"
                     /locus_tag="ACHU9J_002925"
     CDS             4942..5370
                     /gene="tolR"
                     /locus_tag="ACHU9J_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1906415.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5435..6667
                     /gene="tolA"
                     /locus_tag="ACHU9J_002926"
     CDS             5435..6667
                     /gene="tolA"
                     /locus_tag="ACHU9J_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1906416.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6800..8092
                     /gene="tolB"
                     /locus_tag="ACHU9J_002927"
     CDS             6800..8092
                     /gene="tolB"
                     /locus_tag="ACHU9J_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1906417.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8127..8648
                     /gene="pal"
                     /locus_tag="ACHU9J_002928"
     CDS             8127..8648
                     /gene="pal"
                     /locus_tag="ACHU9J_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1906418.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8658..9449
                     /gene="cpoB"
                     /locus_tag="ACHU9J_002929"
     CDS             8658..9449
                     /gene="cpoB"
                     /locus_tag="ACHU9J_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1906419.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9614..9689
                     /locus_tag="ACHU9J_002930"
     tRNA            9614..9689
                     /locus_tag="ACHU9J_002930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9647..9649,aa:Lys,seq:ttt)
     gene            9839..9914
                     /locus_tag="ACHU9J_002931"
     tRNA            9839..9914
                     /locus_tag="ACHU9J_002931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9872..9874,aa:Val,seq:tac)
     gene            9918..9993
                     /locus_tag="ACHU9J_002932"
     tRNA            9918..9993
                     /locus_tag="ACHU9J_002932"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9951..9953,aa:Lys,seq:ttt)
     gene            10140..10215
                     /locus_tag="ACHU9J_002933"
     tRNA            10140..10215
                     /locus_tag="ACHU9J_002933"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10173..10175,aa:Lys,seq:ttt)
     gene            10249..10324
                     /locus_tag="ACHU9J_002934"
     tRNA            10249..10324
                     /locus_tag="ACHU9J_002934"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10282..10284,aa:Lys,seq:ttt)
     gene            10573..>11449
                     /gene="nadA"
                     /locus_tag="ACHU9J_002935"
     CDS             10573..>11449
                     /gene="nadA"
                     /locus_tag="ACHU9J_002935"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1906420.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2845 a         2733 c         3036 g         2835 t
ORIGIN      
        1 tttattagta atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa agaattaagg
       61 tcaaccgtgc tgtttttgct tcgtctcttt ttatctttaa attgccaacc gaaactaatt
      121 tcagccttat aactcacaca ttttaaacat aaatgtcact aaagttacct tattgaaaca
      181 tgattaacat aatttgtagg aattgatatt tatcaatgta taagtcttgg aaattggcat
      241 caaaaagaga taaattgttc tcgatcaaat tggctgaaag gcggtaattt agctataaat
      301 tgatcaccgt cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg taaggatttt
      361 gcggcgtaat atatacgtgg gatcaatttg agtttttatt aacatgtttg caacctttct
      421 ttacgccgtt tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca aatttctatt
      481 ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca
      541 aggagtcatg atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat
      601 gtaccacttc ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga
      661 aacggtctac gtcctctccg gcaaacagat ttataaagat atgaccaagt tctggggcaa
      721 gttgtttggt atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg agttccagtt
      781 cgggactaac tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc
      841 aatcgaaggt ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg
      901 ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc
      961 aaacctgtcc gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc
     1021 cgatttcaac tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa
     1081 cccggttgct caggtgaaat tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat
     1141 gttcatcctc ggtatcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa
     1201 acgctccttt gctatcgctg ccagcttcgg tatggctgct gttctgtctg ttattgttct
     1261 gggtgatgaa tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga
     1321 agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca
     1381 ggaagaggag acgaacaaat ttgcgattca gatcccttac gcactgggca tcattgcaac
     1441 gcgttccgtg gataccccgg ttatcggcct gaaagagctg atggtgcagc atgaagaacg
     1501 cattcgtaac gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga
     1561 ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac ctcggttacg gtctgctgct
     1621 gaaacgctat acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa
     1681 agactccatc ccgcgtgtag cgccgctgta ctttgcgttc cgtatcatgg tggcgtgtgg
     1741 cttcctgctt ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg
     1801 cgagaaaaaa tggcttctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt
     1861 agaagcgggc tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct
     1921 gccgacagct gtggcgaact cgtcactgac cgcaggcgat ctcatcttct caatggtgct
     1981 gatttgcggc ctgtataccc tgttcctggt ggcagaattg ttcttaatgt tcaagtttgc
     2041 acgcctcggc ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt cttccacgac
     2101 tactcagccg gcacgctaag acaggagtcg tcaaatgatc gattatgaag tattgcgttt
     2161 tatctggtgg ctgctggttg gcgttctgct gattggtttt gcagtcactg acggtttcga
     2221 catgggggtg ggcatgctca cccgtttcct cggtcgtaac gacaccgagc gtcgaattat
     2281 gattaactcc attgcaccac actgggacgg taaccaggtt tggctgatca ccgcgggcgg
     2341 cgcactcttt gctgcctggc cgatggtcta tgccgctgcg ttctccggct tctatgtggc
     2401 gatgatcctc gtgctggcgt ctttgttctt ccgtccggtc ggttttgact accgctccaa
     2461 gattgaagaa acccgctggc gtaacatgtg ggactggggc atcttcattg gtagtttcgt
     2521 tccgccgctg gtaattggtg tagcgttcgg taacctgttg cagggcgtac cgttcaacgt
     2581 tgatgaatat ctgcgtctgt actacaccgg taacttcttc cagttgctta acccgttcgg
     2641 cctgctggca ggcgtggtga gcgtagggat gatcattact cagggcgcaa cctatctgca
     2701 aatgcgtacc gtgggcgaac tgcacctgcg tacccgtgca acggctcagg tggctgcgct
     2761 ggtgacactg gtctgtttcg cactggctgg cgtatgggtg atgtacggta tcgatggtta
     2821 tgtcgtgaaa tcgacaatgg accattacgc agcctctaac ccactgaata aagaagtggt
     2881 tcgtgaagct ggcgcatggc tggttaactt caacaacacg ccaattctgt gggctattcc
     2941 ggcactgggt gtggttcttc cgctgctgac catcctgact gcacgtatgg ataaagccgc
     3001 gtgggcgttt gtgttctcct ccctgacgct ggcctgcatc atcctgacag ccggtatcgc
     3061 aatgttcccg tttgtgatgc cgtccagcac catgatgaac gcaagtctga caatgtggga
     3121 tgcaacttcc agccagctga cgcttaacgt catgacctgg gttgcggtgg ttctggtacc
     3181 gatcattctg ctctacaccg cttggtgtta ctggaaaatg ttcggtcgta tcaccaaaga
     3241 agatattgaa cgtaacaccc actctctgta ctaagtaagg agctaaaaat gtggtatttc
     3301 gcatggattc tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt
     3361 gaacacgtcg aatcaggcaa agccggtcaa gaagacatct gatgagtaag attatcgcga
     3421 ccttgtatgc ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc
     3481 tgttagcagg atgtatgttt tgggacccat ctcgttttgc cgcgaagacc agtgaactgg
     3541 aaatctggca tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg
     3601 gctttcgtcc gcagaaggtt ctttggcaag ggattttttg cccattgctt gccgatattg
     3661 ttctcattgt cgggctgatt ttcttcttct tttaaatcag aattctcttt aaaaaattat
     3721 gggccgctcc aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc
     3781 aatcgcgcgc gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg
     3841 taaagtgaat acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc
     3901 cggtggtgtg gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat
     3961 gctgcgtcat catcacttca gtcagcaggc gctgatggct gaacgcgttg cctttgtggt
     4021 acgtaaaatg acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca
     4081 gactgaaata acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc
     4141 cgagaatact ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat
     4201 gaagcctcgt gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat
     4261 atccttgatt tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt
     4321 ggtttttcaa tcgcatcctg ggccattatt atccagcgga cccgtattct taacgcagcg
     4381 gcgcgcgaag ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc
     4441 tatcaagaga gtcaggggaa acgggataat ctgactggtt cggaacaaat attttacagc
     4501 gggttcaaag agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg
     4561 gaaggggcgt cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg
     4621 cacattccgt tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg
     4681 gtctggggga tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa
     4741 atggttgcgc ccggtatcgc agaagcgttg attgcgactg caattggtct gttcgccgca
     4801 attccggcgg taatggctta taaccgcctc aaccagcgcg taaacaaact ggaactgaat
     4861 tacgacaact ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc
     4921 gagagcaaca aggggtaagc catggccaga gcgcgtggac gaggtcgtcg cgatctcaag
     4981 tccgaaatca acattgtacc gttgctggac gtactgctgg tgctgttgct gatctttatg
     5041 gcgacagcgc ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc tactgaatca
     5101 caggcggtga gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag
     5161 tacaccgtgg tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg
     5221 gaagtgtcca gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa
     5281 gatgtgcctt acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa
     5341 tcggttggtt taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag
     5401 cgggtaacag gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga
     5461 caagctcaag cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct
     5521 gctctggagt tcgttcgatg agaatataga agcttcagcc ggaggcggcg gtggttcgtc
     5581 catcgacgct gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag
     5641 ccaggaatca agcgcgaagc gttctgatga gcagcgcaag ataaaggaac agcaggctgc
     5701 tgaagaactg cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga
     5761 gcggttagct gctcaggaac agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt
     5821 aaagcagaag caagcggaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc
     5881 cgaagcggat gctaaagctg cggaagaagc agcgaagaaa gcggcggcag acgcgaagaa
     5941 aaaagcagaa gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc
     6001 cgctgcggca ctgaagaaga aagcggaagc ggcagaagct gctgcagctg aagcaagaaa
     6061 gaaagcggca gcagagaaag ctgcagccga caaaaaagca gcagaaaaag cggctgctga
     6121 taaggcagca gctgataaga aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc
     6181 agcggcaaaa gcagcggcag aaaaagccgc cgcagacaaa aaagcggcag cggcaaaagc
     6241 agcagctgaa aaagccgctg cagcaaaagc tgccgcagag gcagatgata ttttcggtga
     6301 gctaagctct ggtaagaatg caccgaaaac ggggggaggg gcgaaaggga acaatgcttc
     6361 gcctgccggg agtggtaata ctaaaaacaa tggcgcatca ggggccgata tcaataacta
     6421 tgccgggcag attaaatctg ctatcgaaag taagttctat gacgcatcgt cctatgcagg
     6481 caaaacctgt acgctgcgca taaaactggc acccgatggt atgttactgg atatcaaacc
     6541 tgaaggtggc gatcccgcac tttgtcaggc tgcgttggca gcagctaaac ttgcgaagat
     6601 cccgaaacca ccaagccagg cagtatatga agtgttcaaa aacgcgccat tggacttcaa
     6661 accgtaatcg cgatgttgac tgttccgacg gtcaacatca ggcaccggtt gccacggggt
     6721 tctggtagtt ttgtgtattt tagtttgtta acattctgct aaattatcgt gggccatcgg
     6781 tccagataag ggagatatga tgaagcaggc attacgagta gcatttggtt ttctcatact
     6841 gtgggcatca gttctgcatg ctgaagtccg cattgtgatc gacagcggtg tagattccgg
     6901 tcgtcctatt ggtgttgttc ctttccagtg ggcgggccct ggtgcggcac ctgaagatat
     6961 tggcggcatc gttgctgccg acttgcgtaa cagcggtaaa tttaatccgt tagatcgcgc
     7021 tcgtctgcca cagcagccgg gtagtgcgca ggaagtacaa ccagctgcat ggtccgcgct
     7081 gggtattgac gctgtagttg tcggtcaggt cactccgaat ccggatggtt cttacaatgt
     7141 tgcttatcaa cttgttgaca ctggcggcgc accgggtact gtacttgctc agaactcgta
     7201 caaagtgaac aagcagtggc tgcgctatgc tggtcatacc gccagtgatg aagtgtttga
     7261 aaaactgacc ggcattaaag gtgcgttccg tacccgtatt gcctacgttg ttcagaccaa
     7321 cggcggtcag ttcccgtatg aactgcgcgt atctgactat gacggttaca accagtttgt
     7381 cgttcaccgt tcaccacagc cgctgatgtc cccggcgtgg tcaccagacg gttctaaact
     7441 ggcttatgtg accttcgaaa gcggtcgttc cgcgctagtt atccaaacgc tggcaaacgg
     7501 cgctgtacgt caggtggctt cattcccgcg tcacaacggt gcgcctgcat tctcgccaga
     7561 cggcagcaaa ctggcattcg ccttgtcgaa aaccggtagc ctgaacctgt acgtaatgga
     7621 tttggcttct ggtcagatcc gccaggtgac tgatggtcgc agtaacaata ccgaaccgac
     7681 ctggttcccg gacagccaga acctggcatt tacttctgac caggccggtc gtccacaggt
     7741 ttataaagtg aatatcaacg gcggtgcgcc acaacgtatt acctgggaag gttcgcagaa
     7801 ccaggatgcg gatgtcagca gcgacggtaa atttatggta atggtcagct ccaacggtgg
     7861 gcagcagcac attgccaaac aagatctggc aacgggaggc gtacaagttc tgtcgtccac
     7921 gttcctggat gaaacgccaa gtctggcacc taacggcact atggtaatct acagctcttc
     7981 tcaggggatg ggatccgtgc tgaatttggt ttctacagat gggcgtttca aagcgcgtct
     8041 tccggcaact gatggacagg tcaaattccc tgcctggtcg ccgtatctgt gataataatt
     8101 aattgaatag taaaggaatc attgaaatgc aactgaacaa agtgctgaaa gggctgatga
     8161 ttgctctgcc tgttatggca attgcggcat gttcttccaa caagaacgcc agcaatgacg
     8221 gcagcgaagg catgctgggt gccggcactg gtatggatgc gaacggcggc aacggcaaca
     8281 tgtcttccga agagcaggct cgtctgcaaa tgcaacagct gcagcagaac aacatcgttt
     8341 acttcgatct ggacaagtac gatatccgtt ctgacttcgc tcaaatgctg gatgcacatg
     8401 caaacttcct gcgtagcaac ccgtcttaca aagtcaccgt agaaggtcac gcggacgaac
     8461 gtggtactcc ggaatacaac atctccctgg gtgaacgtcg tgcgaacgcc gttaagatgt
     8521 acctgcaggg taaaggcgtt tctgcagacc agatctccat cgtttcttac ggtaaagaaa
     8581 aacctgcagt actgggtcat gacgaagcgg catactccaa aaaccgtcgt gcggtactgg
     8641 tttactaaga gaattgcatg agcagtaact tcagacatca actattgagt ctgtcgttac
     8701 tggttggtat agcggccccc tgggccgctt ttgctcaggc accaatcagt agtgtcggct
     8761 caggctcggt cgaagaccgc gtcactcaac ttgagcgtat ttctaatgct cacagccagc
     8821 ttttaaccca actccagcaa caactttctg ataatcaatc cgatattgat tccctgcgtg
     8881 gtcagattca ggaaaatcag tatcaactga atcaggtcgt ggagcggcaa aagcagatcc
     8941 tgttgcagat cgacagcctc agcagcggtg gtgcagcggc gcaatcaacc agcagcgatc
     9001 aaagcggtgc ggcggcatca acgacgccga cagctgatgc tggtactgcg aatgctggcg
     9061 cgccggtgaa aagcggcgat gcaaacacgg attacaatgc agctattgcg ctggtgcagg
     9121 ataaatcccg ccaggatgac gcaatggtgg catttcagaa tttcatcaaa aattaccctg
     9181 attcaactta cctgccaaac gccaattatt ggctgggtca gttaaactac aacaagggta
     9241 aaaaagatga tgcggcgtac tattttgctt cggtagtgaa aaactatccg aagtcaccaa
     9301 aggctgctga tgcgatgttt aaagtcggcg tcatcatgca ggacaaaggt gacaccgcaa
     9361 aagcgaaagc cgtataccag caggttatca gtaaataccc tggtactgat ggcgctaaac
     9421 aggcacaaaa acgtctgaac gcgatgtaat gcataacaca cgaccagaag tcgcattatt
     9481 tctggtcgtg tcgtgcgaat cataagcagt tgagtgatct gcatcgaaat ttttgttgcg
     9541 ctcaagtctg aaatcagtaa tatatgccgc cgttgccacg ggatatcaaa caaaccgaaa
     9601 gcaacgaaaa agtgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt
     9661 cgcaggttcg aatcctgcac gacccaccag ttttagcatc gaagacagat gttaagcgtg
     9721 taggataacg ttgcgtcagc aacggcccgt agggcgagcg aagcgagtca tcctgcacga
     9781 cccaccacta attacggtgg gttcggtgga agtagtttgt agtatccagc gcagtatcgg
     9841 gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc
     9901 gtcatcaccc accactcggg tcgttagctc agttggtaga gcagttgact tttaatcaat
     9961 tggtcgcagg ttcgaatcct gcacgaccca ccaattttaa catcaaactc agatgttaag
    10021 cgtgaaggat aacgttgcgc cagcaacggc ccgtagggca agcgaagcga gtcatcctgc
    10081 acgacccacc aatcttaaag attggccccg agtagaaatc tttcaggtaa cacccgtatg
    10141 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
    10201 ctgcacgacc caccatcctg aatgattaaa gcagcataat cccgcaaggg gtcgttagct
    10261 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
    10321 accaatgtaa aaaagcgccc taaaggcgct tttttgctat tcaggcatcc tcaatttcac
    10381 tttgtaaacc tgatgacatc gtcagagctt actgtgcaag caactctacg tcggtggaat
    10441 taggcgtaat tcgagtgact tttccccacc attcgactat cttgtttagc atataaaaca
    10501 aattacgccg atagtagcga atattacgct aatgtcggtt ttaacgttaa gcctgtaaaa
    10561 cgagatggta agatgagcgt gatgtttgat ccagacacgg cgatttatcc tttccccccg
    10621 aagccgacgc cgttaagcat tgatgaaaaa gcgtattacc gcgagaagat aaaacgtctg
    10681 ctaaaagaac gtaatgcggt gatggttgcc cactactata ccgaccccga aattcaacaa
    10741 ctggcagaag aaaccggtgg ctgtatttct gattctctgg aaatggcgcg cttcggtgca
    10801 aagcatcccg cttctacgtt gttagtcgct ggggtgagat ttatgggaga aaccgccaaa
    10861 attctcagtc cggaaaaaac aattctgatg ctgacacttc aggctgaatg ttcactggat
    10921 ctcggctgcc ctgttgaaga atttaacgca ttttgcgatg cccatcccga tcatactgtc
    10981 gtcgtctacg ccaacacttc tgctgcggta aaagcgcgcg cagattgggt ggtaacttca
    11041 agcattgccg tcgaacttat tgatcatctg gatagttttg gtgaaaaaat catctgggca
    11101 cccgacaaac atctggggcg ttacgtgcaa aaacagacgg gcgcagacat tttatgctgg
    11161 cagggtgcct gtattgtgca tgatgaattt aagactcagg cgttaacccg cttgcaagaa
    11221 gaatacccgg atgctgccat actggtgcat ccagaatcac cacaagctat tgtcgatatg
    11281 gcggatgcgg tcggttccac cagtcaactg atcgctgctg cgaaaacatt gccacatcag
    11341 cggcttatcg tggcaaccga tcggggcatt ttctacaaaa tgcagcaggc ggtgccagat
    11401 aaagagttac tggaagcacc aaccgcaggt gagggtgcaa cctgccgca
//
LOCUS       DAZPJP010000122        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPJP010000122 DAZPJP010000000
VERSION     DAZPJP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.122"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHU9J_002936"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHU9J_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1906421.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHU9J_002937"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHU9J_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1906422.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHU9J_002938"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHU9J_002938"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1906423.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHU9J_002939"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHU9J_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1906424.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHU9J_002940"
     CDS             complement(3928..5214)
                     /locus_tag="ACHU9J_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1906425.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHU9J_002941"
     CDS             complement(5235..6026)
                     /locus_tag="ACHU9J_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1906426.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHU9J_002942"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHU9J_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1906427.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHU9J_002943"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHU9J_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1906428.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHU9J_002944"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHU9J_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1906429.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHU9J_002945"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHU9J_002945"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1906430.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHU9J_002946"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHU9J_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1906431.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHU9J_002947"
     CDS             complement(9882..10364)
                     /locus_tag="ACHU9J_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1906432.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHU9J_002948"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHU9J_002948"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1906433.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2805 a         2748 c         3059 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcactggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPJP010000123        11425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPJP010000123 DAZPJP010000000
VERSION     DAZPJP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.123"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHU9J_002949"
     CDS             79..213
                     /locus_tag="ACHU9J_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1906434.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHU9J_002950"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHU9J_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1906435.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHU9J_002951"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHU9J_002951"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1906436.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVCCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHU9J_002952"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHU9J_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1906437.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHU9J_002953"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHU9J_002953"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1906438.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHU9J_002954"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHU9J_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1906439.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHU9J_002955"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHU9J_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1906440.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHU9J_002956"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHU9J_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1906441.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHU9J_002957"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHU9J_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1906442.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHU9J_002958"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHU9J_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1906443.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHU9J_002959"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHU9J_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1906444.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHU9J_002960"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHU9J_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1906445.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHU9J_002961"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHU9J_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2807 a         2794 c         2837 g         2987 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttgctgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgttattt
    11341 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    11401 acgccgctga acgcggcgtt ttgga
//
LOCUS       DAZPJP010000124        11410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPJP010000124 DAZPJP010000000
VERSION     DAZPJP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.124"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHU9J_002962"
     CDS             460..1386
                     /locus_tag="ACHU9J_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR1906446.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHU9J_002963"
     CDS             complement(1336..2529)
                     /locus_tag="ACHU9J_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1906447.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHU9J_002964"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHU9J_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1906448.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHU9J_002965"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHU9J_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1906449.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHU9J_002966"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHU9J_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1906450.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHU9J_002967"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHU9J_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1906451.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHU9J_002968"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHU9J_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1906452.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHU9J_002969"
     CDS             complement(11165..11308)
                     /locus_tag="ACHU9J_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906453.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2880 a         2878 c         2934 g         2718 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc
//
LOCUS       DAZPJP010000125        11296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPJP010000125 DAZPJP010000000
VERSION     DAZPJP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.125"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..742
                     /gene="hdhA"
                     /locus_tag="ACHU9J_002970"
     CDS             <1..742
                     /gene="hdhA"
                     /locus_tag="ACHU9J_002970"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1906454.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            963..1553
                     /gene="uidR"
                     /locus_tag="ACHU9J_002971"
     CDS             963..1553
                     /gene="uidR"
                     /locus_tag="ACHU9J_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1906455.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1942..3753
                     /gene="uidA"
                     /locus_tag="ACHU9J_002972"
     CDS             1942..3753
                     /gene="uidA"
                     /locus_tag="ACHU9J_002972"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1906456.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3750..5123
                     /gene="uidB"
                     /locus_tag="ACHU9J_002973"
     CDS             3750..5123
                     /gene="uidB"
                     /locus_tag="ACHU9J_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1906457.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5162..6472
                     /gene="uidC"
                     /locus_tag="ACHU9J_002974"
     CDS             5162..6472
                     /gene="uidC"
                     /locus_tag="ACHU9J_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1906458.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6653..8161)
                     /locus_tag="ACHU9J_002975"
     CDS             complement(6653..8161)
                     /locus_tag="ACHU9J_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1906459.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8262..9437)
                     /gene="manA"
                     /locus_tag="ACHU9J_002976"
     CDS             complement(8262..9437)
                     /gene="manA"
                     /locus_tag="ACHU9J_002976"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1906460.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9636..11282
                     /gene="fumB"
                     /locus_tag="ACHU9J_002977"
     CDS             9636..11282
                     /gene="fumB"
                     /locus_tag="ACHU9J_002977"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1906461.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2651 c         2916 g         2917 t
ORIGIN      
        1 cgacggaaaa tgcgccatca tcacaggtgc gggtgcaggt attggtaaag aaatcgccat
       61 tacattcgcg acagctggcg catctgtggt ggtcagtgat attaacgccg acgcagctaa
      121 ccatgttgta gacgaaattc aacaactggg tggtcaggca tttgcctgcc gttgtgatat
      181 tacttccgaa caggaactct ctgcactggc agactttgct atcagtaagc tgggtaaagt
      241 tgatattctg gttaacaacg ccggtggcgg tggacctaaa ccgtttgata tgccaatggc
      301 ggattttcgc cgtgcttatg aactgaatgt gttttctttt ttccatctgt cacaacttgt
      361 tgcgccagaa atggaaaaaa atggcggtgg cgttattctg accatcactt ctatggcggc
      421 agaaaataaa aatataaaca tgacttccta tgcatcatct aaagctgcag ccagtcatct
      481 ggtcagaaat atggcgtttg acctgggtga aaaaaatatt cgggtaaatg gcattgcgcc
      541 gggggcaata ttaaccgatg ccctgaaatc cgttattaca ccagaaattg aacaaaaaat
      601 gttacagcac acgccgatca gacgtctggg ccaaccgcaa gatattgcta acgcagcgct
      661 gttcctttgc tcgcctgctg cgagctgggt aagcggacaa attctcaccg tctccggtgg
      721 tggggtacag gagctcaatt aatacactaa cggaccggta aacaaccgtg cgtgttgttt
      781 accgggataa actcatcaac gtctctgcta aataactggc agccaaatca cggctattgg
      841 ttaaccaatt tcagagtgaa aagtatacga atagagtgtg ccttcgcact attcaacagc
      901 aatgataggc gctcacctga caacgcggta aactagttat tcacgctaac tataatggtt
      961 taatgatgga taacatgcag actgaagcac aaccgacacg gacccggatc ctcaatgctg
     1021 ccagagagat tttttcagaa aatggatttc acagtgcctc gatgaaagcc atctgtaaat
     1081 cttgcgccat tagtcccggg acgctctatc accatttcat ctccaaagaa gccttgattc
     1141 aggcgattat cttacaggac caggagaggg cgctggctcg tttccgggaa ccgattaaag
     1201 ggattcattt tgttgactat atggtcgagt ctattgtctc tctcacccat gaagcctttg
     1261 ggcaacgggc gctggtggtc gaaattatgg cggaagggat gcgtaaccca caggtcgccg
     1321 ccatgcttaa aaataagcat atgacgatca cggaatttgt tgcccagcgg atgcgtgatg
     1381 cccagcaaaa aggcgagata agcccagaca tcaacacgtc aatgacttca cgtttactgc
     1441 tggatctgac ctacggtgta ctggccgata tcgaagcgga agatctggcg cgtgaagcgt
     1501 cgtttgctca gggattacgc gcgatgattg gcggtatctt aaccgcatcc tgatcctctc
     1561 atttcgcggg ctggtgataa ctgtgcccgc gtttcatact ttaatttccc tgtacaaaat
     1621 acaatctagc cgggatcgga gaattccccc ccaaaaatat tcaccgtagc catatgtcat
     1681 gagagtttat cgttcccaat atgctcgaac gaacgttcgg ttgcttattt tatggcttct
     1741 gtcaacgccg ttttaaagat taatgcgatc aatatcacgc tgtgggtatt gcagtttttg
     1801 gttttttgat cgcggtgtca gttcttttta tttccatttc tcttccatgg gtttctcaca
     1861 gataactgtg tgcaacacag aattggttaa ctaatcagat taaaggttga ccagtatttt
     1921 tatcttaatg aggagtccct tatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa
     1981 aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt
     2041 tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat
     2101 cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc
     2161 tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat
     2221 tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca
     2281 tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt
     2341 gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac
     2401 ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccgggat ccatcgcagc
     2461 gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc
     2521 gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc
     2581 gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact
     2641 ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc
     2701 gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca
     2761 gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt
     2821 ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg ataacgtgct gatggtgcac
     2881 gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac
     2941 gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct
     3001 gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa
     3061 ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa
     3121 gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa
     3181 ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt
     3241 aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc
     3301 gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc
     3361 caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag
     3421 gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg
     3481 cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat
     3541 caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat
     3601 tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca agaaggggat cttcacccgc
     3661 gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc
     3721 ggtgaaaaac cgcagcaggg aggcaaacaa tgaatcaaca actctcctgg cgcaccatcg
     3781 tcggctacag cctcggtgac gtcgccaata acttcgcctt cgcaatgggg gtgctcttcc
     3841 tgttgagtta ctacaccgac gtcgctggcg tcggtgccgc tgcggcgggc accatgctgt
     3901 tactggtgcg ggtattcgat gccttcgccg acgtctttgc cggacgagtg gtggacagtg
     3961 tgaatacccg ctggggaaaa ttccgcccgt ttttactctt cggtactgcg ccgttaatga
     4021 tcttcagcgt gctggtattc tgggtaccga ccgactggag ccatagccgc aaagtagtgt
     4081 atgcatattt gacctacatg ggcctcgggc tttgctacag cctggtgaat attccttatg
     4141 gttcacttgc taccgcgatg acccaacaac cacaatcccg cgcccgtctg ggcgcggctc
     4201 gtgggattgc cgcttcattg acctttgtct gcctggcatt tctgataggg ccgagcatta
     4261 agaactccag cccggaagag atggtgtcgg tataccattt ctggacgatt gtgctggcga
     4321 ttgccggaat ggtgctttac ttcatctgct tcaaatcgac gcgtgagaat gtggtacgta
     4381 tcgttgcgca gccgtcattg aagatcagtt tgcaaaccct gaaacggaat cgcccgctgt
     4441 ttatgttgtg catcggtgcg ctgtgtgtgc tgatttcgac ctttgcggtc agcgcctcgt
     4501 cgttgttcta cgtgcgctat gtgttaaatg ataccgggct gttcactgtg ctggtactgg
     4561 tgcaaaacct ggttggtact gtggcatcgg caccgcttgt gccggggatg gtcgcgagga
     4621 tcggtaaaaa gaataccttc ctgattggcg ctttgctggg aacctgcggt tatctgctat
     4681 tcttctgggt ttccgtctgg tcactgccgg tggcgttggt tgcgttggcc atcgcttcaa
     4741 ttggtcaggg cgttactatg acagtgatgt gggcactgga agctgatacc gtagaatacg
     4801 gtgaatacct gaccggcgtg cgaattgaag ggctcaccta ttcactattc tcatttaccc
     4861 gtaaatgcgg tcaggcaatc ggaggttcaa ttcctgcctt tattttgggg ttaagcggat
     4921 atattgccaa tcaggtgcaa acgccggaag ttattatggg catccgcaca tcaattgcct
     4981 tagtaccttg cggatttatg ctactggcat tcgttattat ctggttttat ccgcttacgg
     5041 ataaaaaatt caaagaaatc gtggttgaaa ttgataatcg taaaaaagtg cagcagcaat
     5101 taatcagcga tatcactaat taataatcaa taaaaataat cagaacatca aaggtgcaac
     5161 tatgagaaaa atagtggcca tggccgttat ttgcctgacg gctgcctctg gccttatctc
     5221 tgcttatgcg gcgcaactgg ctgacgatga ggcgggacta cgcatcagac tgaaaaacga
     5281 attgcgcagg gcggataagc ccagtgctgg cgcgggaaga gatatttacg catgggtaca
     5341 gggaggattg ctcgatttca atagtggtta ttattccaat attattggcg ttgaaggcgg
     5401 ggcatattat gtttataaat taggtgctcg tgctgatatg agtacccggt ggtatcttga
     5461 tggtgataaa agttttggct ttgccctagg ggcagtaaaa ataaaaccca gtgaaaatag
     5521 cctgcttaaa tcaggtcgct tcgggacgga ttatagttat ggtagcttac cttatcgtat
     5581 tccgttaatg gctggcagtt cgcaacgtac attaccgaca gtttctgaag gagcattagg
     5641 ttattgggct ttaacaccaa atattgatct gtggggaatg tggcgttcac gagtattttt
     5701 atggactgat tcaacaaccg gtattcgtga tgaaggggtg tataacagcc agacgggaaa
     5761 atacgataaa catcgcgcac gttctttttt agccgccagt tggcatgatg ataccagtcg
     5821 ctattctctg ggggcatcgg tacagaaaga tgtttccaat cagatacaaa gtattctcga
     5881 gaaaagcata ccgctcgacc cgaattatac gttgaaaggg gagttgctcg gcttttacgc
     5941 gcagctcgaa ggtttaagtc gtaataccag ccagcccaat gaaacggcgt tggttagtgg
     6001 acaattgacc tggaatgcgc cgtggggaag cgtatttggc agtggtggtt atttgcgcca
     6061 tgcaatgaat ggtgccgtgg tggataccga cattggctat cccttttcat taagtcttga
     6121 tcgtaaccgt gaaggaatgc agtcctggca attgggcgtc aactatcgtt taacgccgca
     6181 atttacactg acatttgcac agattgtgac tcgcggctat gaatccagta aacgagatgt
     6241 gcggattgaa ggcgcgggta tcttaggtgg tatgaactat cgggtcagcg aagggccgtt
     6301 acaagggatg aatttctttc ttgctgccga taaagggcgg gaaaagcgcg atggcagtac
     6361 gctgggcgat cgcctgaatt actgggatgt gaaaatgagt attcagtatg actttatgct
     6421 gaagtcagaa ataacgccgg agagaaaaac ctccggcgtt tcagattgtt gacaaagtgc
     6481 gcgtttttta tgccggatgc ggcgtaaacg ccttatccag cctacaaaaa ctcataaatt
     6541 caaagtgttg caggaaaagg taggcctgat aagcgtagcg catcaggcaa tctctggttt
     6601 gttttcagat gaaaacgccg aagtgaaaat tctccggcgt tttggccgtg aattactgct
     6661 gcggaattgc cggtacagcc ggaacgttaa gagctggcat cgcaaacata ccaacgaaat
     6721 cttccagtgg cattttctgc ccgtttaacg ttatctgacc gttagtatat tgcaggctgg
     6781 tggtgatggt attgtcctgc aaggtggtca gacggaacat ctgccccatt gctgatgcac
     6841 cttcaacttg ctgtttcgcc agttttttcg cttgatcttc ctgataacct tccagcttcg
     6901 ctacctgagt cataaactca gttgccatat ccaccggaat ggtcagtttc gcatccagag
     6961 atttaaccga acgatctact tcctgcgcca gcgtttgcgg cgcttcttta gtcgttgccg
     7021 gatctttcag gaacagcgac agattcagcg cactttcacc ctgactgttt ttccagctta
     7081 gcggcgcgat agtaatcacc ggatcgcctt tcaacatcag cggcagggcg ctaaagaagg
     7141 cttccgtcac tttctcctga taaagttcgg gattgttggc aatttctggc tgtgccagca
     7201 gcgcctgagt ttgcgcgtta tattgctggc taaactgatg ccaggcttca ccatcaatct
     7261 ggccgacttt taaagtcagc ttgccgctgc ccagatcctg attctgtacc ttcaggctgt
     7321 ttagcgagta atccagttgg ctattgatcg ttttaccgtc attgaccaga tccgatttac
     7381 cgctgatctc catgccttcc agcagtgcca gttctttgcc ttccactgaa atggtcattt
     7441 tttccagtga cagtttttgg tttcctacgc gctcaccaaa acttgccagc gtgctggaac
     7501 cgtcggtttt cagattatta aaggtcaact gcactttctg gttgtattcg ttaactgcgt
     7561 ctatccgacc actttgcgcc tccccggaaa gggagatggc tttgccgtct ctgtcagcat
     7621 ttaactggaa ctcgccgccg ctaaaggcga ctttttcatc cttttgctcg taattcagtg
     7681 gcttgagcga aatatcggaa ctggaatcac cgctgtaacc aatgcgcgag ttaatctcaa
     7741 aaggcgtttc accttttgcc atatcaaaca gtggtttgct tacttcgtta ttaaccagcg
     7801 tggtttgaat tgatgccatc gacgggatca ggttcagttt tttaagctgg gcaagcggga
     7861 agggaccatg atcaaccgat tcgttgaaga tgacgctctg accgctttta atccacggat
     7921 tttctttccc ggcaatgggt ttcaccaaca gttgcaactg gctgctgaat acgccgcgat
     7981 gatagttttg ataactcact tccaggttgg attcaggagc tgtcagtttg agttgcgcgt
     8041 tcgcctgcgc gaccatgtct tcgagatggg tttcaatctt cttgcctgta taccatgcgc
     8101 cgcctgtcca gactacgcct agcgcaacaa tgacgcctac cgctaccagc gatttattca
     8161 taatgattat ccataaaatg aaatcaggcg gactggccgc ctgaaggtgt tataagcctt
     8221 taataagctt agcaagagat gttaattttt tcagtaagct cttacagctt gttgtaaaca
     8281 cgcgctaaac ggccgtggcc tttgacagtc accggtgatt cgttggcggc aataaacgct
     8341 gattcacccg gtttaagctg taactgctga gaacctttcc acaacgttgc atcgccttcg
     8401 acgcagaaca aaatggcggc actctgctgg ctaatggtgg tttctttatc actaaggtca
     8461 tgcagcgaga aggcaaaatc atccactgga atcgggaagt ccagttctgc accttgtttc
     8521 accggctggg tcaacaactg gttagccggt ttggcttcga atttcacatt ggcaaccagt
     8581 tccggaatat caatgtactt aggcgtcaga cccgcacgca gcacgttatc ggagttcgcc
     8641 atcacttcca gcgccacgcc ttgcaggtaa gcgtgcggtg tttcagcgaa caggaacatc
     8701 gcttcgccag gattcaattt caccacattc agtaacagcg gggagaacag gccactgtct
     8761 tccgggtaaa attcagaaat taaacgaatc gtttgccacg gttcaccctg ctgactgtcg
     8821 agcgccgatt ttaaaatcgc cagcgcgcgg gatttttctt caccctgcat attcaacagg
     8881 ctggcgaaca gttcgcttaa acgttcggca ttaggctgtt gtaaaaagtg agcaattgcc
     8941 ggatgtgcac ctgcgactgg ctggagtagg gagacaatct cggaaaactc acgaaacgcg
     9001 ttcatcgcaa ggaaaggcgt cagcgcaaaa accagctccg gcttgtggtt aggatcttta
     9061 tagttacgct cggcggcatc catcgggata cctgcggcat tttctttggc aaaaccgatt
     9121 tcagaattgc gtttgtttgg atgaacctga atggagagcg gctgtgctgc gcataatact
     9181 ttgaacagga aaggcagttc gccaaagcgt ttggcaacgg cctctccgag cagagtcgat
     9241 ttatcactct caatcacatc acgcagtgaa acgatatctc cggcggcatt ctgcactcgt
     9301 gaactgcttt tcggatgtgc gcccatccac agctcggcca tcggctggct ggacggattt
     9361 tccataccat aaagttcagt caacgccgtt ttgctgcccc aggcatagtt ttgcactgag
     9421 ttaatgagtt tttgcatgat caatccctgt tttaatgtgg aaattaatcc cactattaaa
     9481 gcaagaatcc tacgggaagt aacctggagc cgcaaaaagt cgtactagtc tcagtttttg
     9541 ttaaaaaagt gtgtaggata ttgttactcg cttttaacag ggcaacggaa cacccgccca
     9601 gagcataacc aaaccaggca gtaagtgaga gaacaatgtc aaacaaaccc tttcattatc
     9661 aggctccttt tccactcaaa aaagatgata ctgagtatta cctgctaacc agcgaacacg
     9721 ttagcgtatc tgaatttgaa gggcaggaga ttttgaaagt cgcacccgaa gcgttaactc
     9781 tgttggcgcg tcaggcgttt catgatgcgt catttatgct gcgtccggct caccaacaac
     9841 aggtggccga cattctgcgt gacccggaag ccagcgaaaa tgataaatat gtggcgctgc
     9901 aattcctgcg caactccgac atcgcggcga aaggtgttct gccaacctgt caggataccg
     9961 gcactgcgat tattgttggt aaaaaagggc agcgtgtatg gaccggtggc tgtgatgaag
    10021 cggcgctggc gcgcggtgtc tataacactt atatcgaaga taatttgcgc tactcgcaaa
    10081 acgcgccgct ggatatgtat aaagaggtga ataccggcac caatctgcca gcgcagatcg
    10141 atctttatgc cgttgatggc gacgagtaca aattcctctg tatcgccaaa ggtggtggtt
    10201 cggcaaacaa gacgtatctc tatcaggaaa ccaaagcgtt actaacgccg gggaaactga
    10261 aaaattacct ggttgagaag atgcgcacgc tgggtacggc ggcctgtcct ccgtatcata
    10321 ttgcgttcgt aattggtgga acttctgcag aaactaacct taaaacagtg aaactggctt
    10381 ccgctaaata ctatgacgaa ctgccaacgg aagggaatga gcacggtcag gcattccgcg
    10441 atgtggaact ggaaaaagaa ttgctgatcg aagcgcaaaa tctcggtctg ggtgcgcagt
    10501 ttggtggtaa atacttcgct cacgacatac gcgtgattcg cctgccacgt cacggcgcat
    10561 cctgtccggt tggtatgggt gtttcctgct ctgctgaccg taatatcaaa gcgaagatca
    10621 accgtcaggg gatctggatc gaaaaactgg aacataatcc aggtaaatat atccctgaag
    10681 agctgcgtaa agcgggagaa ggcgaagcgg tgcgcgttga ccttaaccgt ccgatgaaag
    10741 agatcctcgc acagttgtcg cagtatcccg tttctacacg cttatcgctt aacggcacga
    10801 ttatcgtcgg tcgtgatatt gctcacgcca aactgaaaga gcggatggat aacggtgaag
    10861 ggctgccgca gtacattaaa gatcatccga tttactacgc gggtccggcc aaaacgccgg
    10921 aaggttatgc ctccggttct cttggcccaa cgaccgccgg acggatggat tcttatgtcg
    10981 atcaactgca agcgcagggc ggaagtatga tcatgctggc gaaaggcaac cgcagccagc
    11041 aggtgacgga tgcctgtaaa aaacacggcg gcttctacct tggcagtatc ggtggtccgg
    11101 ccgctgtatt ggcgcaggga agtattaaga gcctggaatg tgttgaatat ccggaactgg
    11161 gaatggaagc catctggaaa attgaagtgg aagatttccc ggcgtttatc cttgtggatg
    11221 ataaaggaaa tgacttcttc cagcagatac aactcacaca gtgcactcgc tgtgtgaaat
    11281 aaacagagcc gccctt
//
LOCUS       DAZPJP010000126        11246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPJP010000126 DAZPJP010000000
VERSION     DAZPJP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.126"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1760
                     /locus_tag="ACHU9J_002978"
     CDS             <1..1760
                     /locus_tag="ACHU9J_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1906462.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHU9J_002979"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHU9J_002979"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1906463.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHU9J_002980"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHU9J_002980"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1906464.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHU9J_002981"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHU9J_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1906465.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHU9J_002982"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHU9J_002982"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR1906466.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="ACHU9J_002983"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="ACHU9J_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1906467.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="ACHU9J_002984"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="ACHU9J_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1906468.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHU9J_002985"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHU9J_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1906469.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="ACHU9J_002986"
     CDS             complement(10054..10212)
                     /locus_tag="ACHU9J_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1906470.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="ACHU9J_002987"
     CDS             10395..10694
                     /locus_tag="ACHU9J_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1906471.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHU9J_002988"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHU9J_002988"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1906472.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAZPJP010000127        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPJP010000127 DAZPJP010000000
VERSION     DAZPJP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.127"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHU9J_002989"
     CDS             <1..309
                     /locus_tag="ACHU9J_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1906473.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHU9J_002990"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHU9J_002990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1906474.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHU9J_002991"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHU9J_002991"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1906475.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHU9J_002992"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHU9J_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1906476.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHU9J_002993"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHU9J_002993"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1906477.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHU9J_002994"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHU9J_002994"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1906478.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHU9J_002995"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHU9J_002995"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1906479.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHU9J_002996"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHU9J_002996"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1906480.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHU9J_002997"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHU9J_002997"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1906481.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHU9J_002998"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHU9J_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1906482.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHU9J_002999"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHU9J_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1906483.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="ACHU9J_003000"
     CDS             10354..>11242
                     /locus_tag="ACHU9J_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR1906484.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZPJP010000128        11147 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPJP010000128 DAZPJP010000000
VERSION     DAZPJP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.128"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..443
                     /locus_tag="ACHU9J_003001"
     CDS             <1..443
                     /locus_tag="ACHU9J_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1906485.1"
                     /translation="FSKCLFHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSIC
                     ARDIYNMSAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDK
                     SIEILLLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            654..977
                     /locus_tag="ACHU9J_003002"
     CDS             654..977
                     /locus_tag="ACHU9J_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1906486.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            974..1804
                     /gene="amiD"
                     /locus_tag="ACHU9J_003003"
     CDS             974..1804
                     /gene="amiD"
                     /locus_tag="ACHU9J_003003"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1906487.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1801..2814)
                     /locus_tag="ACHU9J_003004"
     CDS             complement(1801..2814)
                     /locus_tag="ACHU9J_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1906488.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2913..4343)
                     /locus_tag="ACHU9J_003005"
     CDS             complement(2913..4343)
                     /locus_tag="ACHU9J_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1906489.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4354..5355)
                     /gene="ltaE"
                     /locus_tag="ACHU9J_003006"
     CDS             complement(4354..5355)
                     /gene="ltaE"
                     /locus_tag="ACHU9J_003006"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1906490.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5392..7110)
                     /gene="poxB"
                     /locus_tag="ACHU9J_003007"
     CDS             complement(5392..7110)
                     /gene="poxB"
                     /locus_tag="ACHU9J_003007"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1906491.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7243..8211)
                     /gene="hcr"
                     /locus_tag="ACHU9J_003008"
     CDS             complement(7243..8211)
                     /gene="hcr"
                     /locus_tag="ACHU9J_003008"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1906492.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8223..9875)
                     /gene="hcp"
                     /locus_tag="ACHU9J_003009"
                     /gene_synonym="priS"
     CDS             complement(8223..9875)
                     /gene="hcp"
                     /locus_tag="ACHU9J_003009"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1906493.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10019..10918)
                     /gene="lysO"
                     /locus_tag="ACHU9J_003010"
     CDS             complement(10019..10918)
                     /gene="lysO"
                     /locus_tag="ACHU9J_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1906494.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2715 a         2929 c         2941 g         2562 t
ORIGIN      
        1 atttcagcaa atgtcttttt catgactgta gtatgcatgg tgtcaagata aaaccatggt
       61 tgccagtaaa atggacaaaa gagttaataa atgattattt gtatggctgt ttactatctc
      121 tgtactctat ctgtgcaagg gatatatata atatgagcgc cgggaataac gtgaaggttg
      181 ctgcggatgc tttccttgaa attattttct ctcttaagaa taaatattgc attaaactat
      241 taagcgctca ggaacgggcg tttatttacg aatttgcaag aatgatcttc gcttatatta
      301 atgacaaaag cattgagatc ttattacttt cttgttttgc ggcgtctgac caaaaagcaa
      361 tccagaggta tatacctcag tcacaagatg gggaagactt taggagtcat ttacaatata
      421 agttgcctct ttccgctcat taatgttgtt ggtgcaacat aataagacag gtaagcggta
      481 ccctataatg aaaccaatat cgggttatgc aacggataat cgtaaagact taagtgggtg
      541 tttttgacac aatgagatga tttcctaaac tgatcaaaac cttcttccca acatggcata
      601 tcagttcgat aatgctttag agatcgaagg cttaacaaac aaggagatcg atcatgcaat
      661 tttctacaac cccaactctg gaaggccaga ccatcgttga atattgcggt gtggtgaccg
      721 gcgaagcgat tttaggtgcc aatattttcc gtgatttctt tgccggtatc cgcgatatcg
      781 ttggcggacg ttccggtgcg tatgaaaaag aactgcgtaa agcacgggag atcgcctttg
      841 aggaattagg ctcccaggcg cgggcgctgg gggccgatgc cgtcgtcggt attgatatcg
      901 actacgaaac ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg
      961 tgaaaacgcg tcgatgagaa gagtcttctg gctggtcgct gccgctctgt tattggcagg
     1021 gtgtgcaggc gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacgcca
     1081 ggcgcaggcg gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt
     1141 tgatagctcg ctggcgacac tgaccgataa gcaggtcagc tcgcattatc tggtccctgc
     1201 ggtaccaccg cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact
     1261 ggcctggcat gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat
     1321 tggcattgag ctggaaaacc gtggctggca aaaatcggcc ggagtgaaat attttgcccc
     1381 gtttgaaccg acacagattc aggcgcttat tccactggcg aaagatatta ttgcccgtta
     1441 tcacatcaag ccggaaaacg tagtggcaca tgcggatatc gcaccgcagc gcaaagacga
     1501 tccggggcca ttatttccct ggcagcaact ggcgcagcag gggattggtg cctggccgga
     1561 tgcgcagcgg gttaactttt accttgccgg gcgcgcgccg cacacaccgg tagataccgc
     1621 gtcattgctg gagcttttgg cgcgctacgg ttatgacgtt aaacctgata tgacaccgcg
     1681 cgagcagcgg cgcgtgatta tggcattcca gatgcatttc cgcccgacgt tatataacgg
     1741 cgaagcggat gcagaaactc aggcgattgc cgaagcattg ctggagaaat acgggcagga
     1801 ttagcgcggc agttttccgt ggtcgcgtag ccaggcggca gttttctcga taccttcatc
     1861 tagggtgatg atcggctgat aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa
     1921 gtcaaaattc aacttggaga cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg
     1981 gcctaaacgc tccatgctgc gggcgatcat atccagcatc gggtagggga cggaacgaat
     2041 acgacagtca atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg
     2101 ctcgccgttg gtgatattgt acacacgccc ggaaggtagc ttatcgcagg cttcctggct
     2161 tgccagccac attgcgtgca cggtattttc atagtaggtc atatcgacca gcgcactgcc
     2221 gccatgcggt aacagaatac tgccgtagtg gtgcatcata tgcgccagac ggggaataaa
     2281 gactttatcg tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg
     2341 attcgcctgc gaaagcatat tgatcacttc ttcgctggct gctttgctgc gggcaaactc
     2401 gttggcgaag cggtgagggc gaaaatcttc tttaatatcg cgatggtggt gataatcgaa
     2461 gtacagggag ggggaagaga tatgaataaa gttacgcaca ccccaggcga cagcccattc
     2521 acccaggcgg cgagtggcgc gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg
     2581 tgaggtaaag ctggagcagt gccacagcgt atcaatgccc gcgagcatca ctttagcttg
     2641 tgatgaaacc agctcggtca gatccgccgg aacaaactct gcgcccattt tttccagcaa
     2701 tttgcccatt gcctcgttgc gaccggtcgc tcgcacgctg atgcctttct ggcataaaaa
     2761 ctctaccgcg tttcgaccta agccgctggt ggcgccggta accagtacct tcatatcaat
     2821 ccactgttgt tgagaaaatg acgtgcgcat tcttccgtga tttcccccat gatgcaatgg
     2881 gaaacatgaa agaataacgc aggttttgtc gattaatctg tgctttgttc tgccagtctg
     2941 gcgatttgtt ttgccattcc gcgaaaaata aacagatgcg cggggatcat caataaccag
     3001 taaaacagcc ccggcatacc gtgcggatgc cagaaagcgc ggacatcgat agtacgatag
     3061 tcgcctttat cttccaggct aaaacacagt cgtcccagcc ccgacgcttt catgccaaat
     3121 aacaacgtaa gttgtttttc cggttcaacg acaatcactt tccagctatc caccgcatcg
     3181 ccagtctgta aatattcgcg ctccgggcgg cctttcgcca gcttatgacc gatcgcgcgg
     3241 tccatcaacg cccgtgtctg ccacaaaata ttgccaaaga aataacgctc tttaccgccg
     3301 atttggttca ctacctgcca taaagcagca aggctggcgg acgttttaac ggtaaacccc
     3361 gcctgtttgg caaaataacc gtactccggt cgccagcggg caaaggcctg agcgtcgtag
     3421 ccccagtcgc tggagttgac cagtttttcc tcctctttca acgtgctacg taccgcgtca
     3481 tcgaaagcga tcagccgttg tgggatgagt gcacgtagcg cggtatcatc cgccagcaga
     3541 tcgtgtttca gcccctgaat caacgccctg gcggtggtgg gcggtacgga agtaatcaca
     3601 ttgagaaacc acaccgaaat ccagcgggtg gggaggggga tggggatcaa ccagcggcgc
     3661 ttaccgctca ccgccataaa atgttcaaac tgttgctgat aactgagcac ctctggtccg
     3721 gcggcttcga agatgcggtg ttcgctggcc ggatgatcta acaacgccac cagatagtgc
     3781 agcaagtttt ccagcgcgat gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac
     3841 actggcaggt tgtagaccat atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg
     3901 ataattccgg cccgaagttc ggtcacaggt acattcgctt cacgaagaat gtccgccgta
     3961 gcctgacgag cacgcagatg atccgactgc tcatgtggcg gggcctgcaa cgaactgaga
     4021 aagattaatt gcttaactgg tacttcacgt agcgcatcgc ggacgttgag agccacctgg
     4081 cgctcctgag cgataaaatc gccgccttcg cccatgctgt gcaccagaaa atagacggta
     4141 tcgatatcct gcaacagggc cggaaggtta tccggccagc tgagatcgac tttatggcaa
     4201 ctgacgtttg ccagttgcag ctttgcaagc ctgtcgacat gacgtgccgc cgccaggatc
     4261 tgatgccctt gctggctgag tgtgcgcacc agatgctgac caatgtagcc actggcaccg
     4321 agaactaaaa tgcgttgcgg cacgtctctc tccttaacgc gccaggaatg cgcgccagtg
     4381 ggcggcgact tccgccagtt gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat
     4441 cggcgaggcg ttaatcagca cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt
     4501 ttcttccccg acgcgaacaa acagcatatt agtgtcctga cgcatcacat ccgcgcctgc
     4561 ttcacgcagt tgctccgcca tccaggcggc gttgtcgtga tcttcctgca accgtgcgac
     4621 gttatttttc agggcataca tcccggcggc agccagaatc ccggactgac gcatcccgcc
     4681 accggccatt ttccgccagc ggatggcacg tttaatgtaa tcacgattac cgacgagtaa
     4741 tgaaccgact ggcgtcccaa gacctttcga caggcaaatg gtgaacgaat cacaatattg
     4801 cgtgatctct ttcagttcgc agccgtaagc caccacggca ttaaagatgc gcgcaccgtc
     4861 aacatgcagc gccagattgc gcttgcgggt aaattcccat gcttctttca ggtattcacg
     4921 cggcagcact ttgccgttgt gggtgttttc tagactgagt aatttggtgc gggcgaaatg
     4981 gatatcgtcg ggtttgattt tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc
     5041 cgcgtcgatg ggttgcggtt gaatactgcc cagcaccgct gcgccaccgg cttcaaacag
     5101 ataattatgc gcggcctgac cgacaatata ctcttcgccg cgttcgcagt gactgagcag
     5161 agcgaccagg ttagcctggg tgccggtcgg cagaaaaatg gcggcttctt taccggaaag
     5221 ctctgcggcg tagtcctgca gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc
     5281 aaccggggcg gccatcattg cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact
     5341 gcgtaaatca atcatggcat gtccttatta tgacgggaaa tgccaccctt tttaccttag
     5401 ccagttcgtt ttcgccagtt cgatcacttc atcgccgcgt ccgctgatga ttgcgcgcaa
     5461 catatacaag ctaaatcctt tggcctgttc gagtttgatc tgcggtggaa tggctaactc
     5521 ttctttggcg accaccacat ccaccagcac cggaccgtcg atggagaagg cgcgttgcag
     5581 ggcttcatca acttcagacg ctttttctac acggataccc gtaatgccgc acgcttcggc
     5641 aatgcgggca aagtttgtgt cgtgtagttc ggtgccgtca gtcaaatagc caccagcttt
     5701 catctccatc gccacaaagc ccagcacgct gttgttaaag acgacaattt tcactggcag
     5761 tttcatctgc actactgaga ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca
     5821 catggcgacc acctgacgtt ctggctctgt cgcctgcgca cccagcgcct gcggcatggc
     5881 gttagccatc gaaccgtggt taaacgaacc taacaggcga cgcttgccgt tcatttttag
     5941 ataacgtgcc gcccacaccg ttggcgtacc aacgtcacag gtgaaaatag cgtcatcggc
     6001 ggcaaaatga ctaatttgct gcgccagata ttgcgggtga atggctttct cgctcggttt
     6061 agctaaatcg tccagccctt tgcgggcgtc gcggtaatct tccagcgctt tatccagaaa
     6121 cttgcgatcg gctttttctt ccaccaatgg aagcaatgca cgcagagtcg acttgatatc
     6181 gccgaccagt gccatatcca ccttgctgtg agcgccgatg ctggctgggt tgatatcaat
     6241 ctgaatgatt ttggcatcgg tcgggtagaa ggcgcggtag ggaaattgcg tgccgagtag
     6301 cactaacgtg tcggcgttca tcatggtatg gaaacctgac gagaagccga ttaacccggt
     6361 cattccaaca tcatacggat tatcgtattc gacatgttct ttaccgcgca gggcatgaac
     6421 aataggcgct ttaattttcc cggcaaactc aactaactct ttatgcgccc ccgcgcagcc
     6481 gctgccacac atcagggcga tattgctgga ataacgcagc agttgcgcca gtttgcgtaa
     6541 ctcttcttct tccggcgtca cgactggttg tggcgcatga taccagtgcg tggttgcccc
     6601 ttctggcgca ggttttaacg ccacgtcgcc tggtaacacg acaaccgaaa cgccacggtt
     6661 aagcaccgct ttgcgcatgg caatcgccag tacttgtggg atctgctccg ggctggaaac
     6721 cagctcgcaa tagtgactac attcgcggaa tagctcttgt gggtgggttt cctggaaata
     6781 gccgctgcca atttcgctgg agggaatatg agcggcaatc gccagtaccg gaacgtgatt
     6841 gcggtggcaa tcgaacaggc cgttgattaa gtgcaggttg ccggggccgc acgatccggc
     6901 gcagaccgcc agttctccgc taagttgtgc ttcagcgcca gcggcaaagg ccgccacttc
     6961 ttcgtggcgg gtggacatcc actcgatggt gcccatgcga ttaagactgt cactaagacc
     7021 gttcagagag tcgcctgtga ctccccagat gcgtttcacc cctgccgatt cgagtgtttt
     7081 ggcgatataa gctgcaaccg tttgtttcat ggttctccat ctcctgaatg tgataacggt
     7141 aacaagttta gttcatctga cggaggggga agggatggga gagaaaggag gcactaacgg
     7201 ttaaatagcc cgatgaaagg aatatcatcg ggcataaggc gattatgcga gaaccaaatc
     7261 cccctgcgga tggcaggagc aggccagtac gtaaccttca gcgatttcgg cgtcggtcag
     7321 cgtcattgta ctgctcaccg tatattcacc ggaaaccact tttgtcttac agcagccgca
     7381 aacacccgca cggcaggcag cgacaaccgg aacgttattg ctttccagcg cctccagtag
     7441 cgtggtgcca accggggcgt aaaattctcg tgccggttgc agtttggtga atttcagacc
     7501 gctggtcgcc gcttctgcta ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc
     7561 gagcgctttc acttcctgct ctacccaatc catatacgga gccgggccgc aggtcatcac
     7621 ggtacgtgaa gctaagtcag gtacacctgc cagcagttcg cgagtgagac gaccagcgat
     7681 aaagccttcg gtaacgttat tttctgccac cagcgttacc ggatagttac gccactcatc
     7741 ggcgaaaata acatcctgcg gcgtacgcac gttgtagatc acccgcacat cggcctgtgg
     7801 acggttcttc gcaagccagc gacgcatcga cataatcggc gtgacgccgc agcctgccgc
     7861 cagcaacagg aatttatctt ctgctttatc gtcgcaggta aattccccca tcgcgtccga
     7921 aagccagaga taatcaccgc gttttacatc gcgcgtcagc cactgggagc cgacaccgtc
     7981 atcaatccgc cgcacggtca gggtgatata ttcactcacg cctggcgtgg aggaaatggt
     8041 gtaagcacgc agcgtttccg ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg
     8101 atatgggtag taatcgtggc aaatcaggga aatcgtccac acatccggcg tttcttgcgt
     8161 aatgtgatga acctgcatcc gccacgggca ttgattcgtt ggcatcgtca tcgacaaact
     8221 ccttacgcgc tcaacagttg cttcatgtct tcttcaacag tggtgataga acgcaggccg
     8281 aatttctcgt tcagcaccgc cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg
     8341 gtgacgatat ttttcacgcc cagagaaagc agcgtcagca gaatgacgat cgctttctgt
     8401 tcaaaccagg agagcaccag cgacagcggc agatcgttca caccacagcc cagtttctct
     8461 gccagagtga cagccagaat aatcgctgag taagcatcgt tacattgacc tgcatctacc
     8521 agacgcggca gaccttcgat atcgccaaac tcaagtttgt taaagcgata tttaccacag
     8581 gcgagggtca ggatcaggca gtcatccggc acgctggtgg cgaaatcggt gaagtagtgg
     8641 cgctcgccgc gtgcgccgtc acagccacca agcaggaaga tatgacgcag tttttcacgg
     8701 ctcaccagat caatcagcgt atcagcagcg ccaagcagcg tctggcgacc aaaacccacg
     8761 gtgataaggt gcggaatttc gctgtacggg aagcctgcca tctgttgcgc ctgggtgata
     8821 accgcagaga aatcatcacc gtccagatga cgcacgccag gccagccaac aatgctgcgg
     8881 gtccagatac gatcgtcata agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc
     8941 acgatggggc cagggaaacg agcgaactcc acttgctgat tctgccagcc gctgccgtag
     9001 ttaccgacca gatgcttgaa tttacgcagc tccgggtagc catgcgcagg cagcatttcg
     9061 ccgtgggtgt agacattaac gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct
     9121 ttgagatcgt gaccggaaat cagaatgcat ttacccgccg tcgctttgac gttgacctgg
     9181 gttggcgtcg ggtgaccgta tttaccggtt tcgcctgcat ccagaatgct catcactttg
     9241 aagttcatct ggccgatttc cattgaacac tcaagaagcg cgttcatatc ggcaggccag
     9301 gtccccagcc acgccatgat tttatggtac tgggcataaa tatcgttgtc gtattgaccg
     9361 agaacatgcg cgtgttccat ataggccgcc gcacctttca ggccatacag gcacagcaga
     9421 cgcaggccga gaatgttttc gccaatcgcc gctttatctt tgttaggggt aaattctgct
     9481 gcctgacgtt gcagctcgcc gagatcatcg ctcaccagtt gcaggtcagc catcgggtta
     9541 tcgacgcgcg cgttggcatc tacagccagg cattgtgctt tcagcgcctc gcgcaaggca
     9601 atcgcttcac gagcgtagcc gacaatacgc ggagaatcga agttaacgtt ggtcagggtt
     9661 gagaaaaagg cacgtggcgc gaagctgtca acatcgtggt tgatgatgcc gtattcacac
     9721 gcttttaccg cccaggcaga aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca
     9781 gaagtttccg ccgttttacc acacatcccc tgcgcgtatg agcagccgtt tcctgccgga
     9841 gtacggatag tttgttcaca ttgcacacaa aacatgatca caccttttaa agttatattt
     9901 aatatacatg tttaaggtta agacgcttaa cgcggggata aaagggattt ttcatgcaac
     9961 tttaagggag attgatttag cgcaattttg gcggcaggga tctaccgcca gagaggtatt
    10021 acgcagagaa aaaggcgatg aggatcggca ctaacaggct aagaataaaa ccgtgaacaa
    10081 ttgccgccgg gaccatatcc agcccgccag tacgttgaag aacgggcagg gtgaaatcca
    10141 ttgatgtggc accgcataag cccagtgcag tagagcggct gcggcgaatc agcccaggga
    10201 tcaacataat agcaatcagt tcacgggcca gatcattaaa aaacgccgcg ctcccgatta
    10261 ccggaccaaa agattcggtc aataaaatac cggaaagaga ataccagccg aaaccggagg
    10321 ccattgccag cgcggtattg atggggagat caagaataaa ggcgttaatt aaaccaccaa
    10381 ttaatgaact gacaaccacc accacggcga caatcattcc ccggcgatta aggacaatct
    10441 gctttaaggt catgccatta ttgcgcaact gaataccaac gaggaaaagt agcaaaatta
    10501 acgtgtattc actggcttcg gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg
    10561 caaaaccaat cactactacg ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg
    10621 acgggagttt ttcttgctga tggtggttgc gccacggcag gcctcgctcc agccacatca
    10681 gggcggcaat attacacagt aaaataacgg taatactgac ggcagaataa tgcagaatcg
    10741 ccaacaggtt actggcgagg ttatcgagaa acgccagact gatacccata aaaaagagaa
    10801 taaggtaaac catccagctt aatagctgat taataacttt taacgcagct tgttggcgaa
    10861 gcggaatgag gtaacctaca atcaggggaa ccagaatgat taacagccca gaaaacatga
    10921 aaacccagtc cttgcaaaga tgaagtcgaa atgcgcgatg acacactact gaaagcggaa
    10981 ggacgagtaa agttgcaatt aaaaggaaat gttatgcata aggagcagta gagtattcgt
    11041 tttcatttaa agatattctt gcgctttaat tacaaactgc accgatgttg gtggcgtcaa
    11101 aatcgccgag gcgttccctg aaggccgggg cagcccacat ggatgtg
//
LOCUS       DAZPJP010000129        10939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPJP010000129 DAZPJP010000000
VERSION     DAZPJP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.129"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHU9J_003011"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHU9J_003011"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1906495.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHU9J_003012"
     CDS             complement(643..882)
                     /locus_tag="ACHU9J_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1906496.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHU9J_003013"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHU9J_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1906497.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHU9J_003014"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHU9J_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1906498.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHU9J_003015"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHU9J_003015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHU9J_003016"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHU9J_003016"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHU9J_003017"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHU9J_003017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHU9J_003018"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHU9J_003018"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1906499.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHU9J_003019"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHU9J_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1906500.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHU9J_003020"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHU9J_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1906501.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /gene="yecU"
                     /locus_tag="ACHU9J_003021"
     CDS             7047..7223
                     /gene="yecU"
                     /locus_tag="ACHU9J_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1906502.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /gene="yecF"
                     /locus_tag="ACHU9J_003022"
     CDS             7210..7434
                     /gene="yecF"
                     /locus_tag="ACHU9J_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1906503.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHU9J_003023"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHU9J_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR1906504.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHU9J_003024"
     CDS             complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHU9J_003024"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1906505.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHU9J_003025"
     CDS             complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHU9J_003025"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1906506.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHU9J_003026"
     CDS             complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHU9J_003026"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1906507.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2713 a         2839 c         2578 g         2809 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     8281 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
     8341 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     8401 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     8461 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
     8521 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
     8581 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     8641 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     8701 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
     8761 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     8821 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     8881 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     8941 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
     9001 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     9061 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     9121 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     9181 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     9241 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     9301 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
     9361 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
     9421 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     9481 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     9541 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
     9601 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     9661 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     9721 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
     9781 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     9841 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     9901 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     9961 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    10021 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    10081 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    10141 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    10201 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    10261 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    10321 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    10381 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    10441 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    10501 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    10561 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    10621 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    10681 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    10741 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
    10801 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
    10861 tgcagtggca taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa
    10921 aacgcgtaaa taaaaaagg
//
LOCUS       DAZPJP010000130        10912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPJP010000130 DAZPJP010000000
VERSION     DAZPJP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.130"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHU9J_003027"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHU9J_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1906508.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHU9J_003028"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHU9J_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1906509.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHU9J_003029"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHU9J_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1906510.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHU9J_003030"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHU9J_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1906511.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHU9J_003031"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHU9J_003031"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1906512.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHU9J_003032"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHU9J_003032"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1906513.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHU9J_003033"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHU9J_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1906514.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8620)
                     /locus_tag="ACHU9J_003034"
     CDS             complement(7754..8620)
                     /locus_tag="ACHU9J_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1906515.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="ACHU9J_003035"
     CDS             complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="ACHU9J_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1906516.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2723 a         2862 c         2682 g         2645 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gaactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc cactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcta tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcgattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgtg
     1681 ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcctcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ctggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagt
     1981 agcatttcgc caaacgcaac caaagagttc ggtctggtat cttccgtgtc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggcca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagcccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactcca gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggctaaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactgc tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcaagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtacc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atgttctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggactga ttctggcggc gcaaaccgaa ggctacacgt gcgatggcgt aacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtaca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttgaacg atctggtcag tcgcctgacg agcacgctgg ataacgaaag gttatttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccac aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gccgttgaag atgaaggacc gggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag tacgtagcaa accagatata aagaaagctg
     5821 ctgaccacca gcacactgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atatcatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcaac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc cgcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcgaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccacca
     7801 aacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac tgtttgccag cgttgggtca
     7861 ctgaccagca aacgggctgt ttatctgccg tggaccactt tcccagccaa cttaaaaaat
     7921 cagcatgctc ccctcccgaa gaggcaaacc actgatggta atagtgacgc gtggtcggtg
     7981 ccgcattgat actctctgcc agatgttgtg tcgctttctc gcgcaaatca cccagtaaca
     8041 acaccaccgc ttgcgggctt cccagacggc aggcatcgcc aaatgcaccc cagcgcaact
     8101 cctccagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc ccagcacgcc
     8161 agcgggtaaa aatttgctcg ctctgatcac ctgcctgcac cagtaggcct cgttgcgtta
     8221 acgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg gcaatttgct
     8281 gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc agcgccagat
     8341 gctgcgcgat ctgcgtcagc gccagatgac cgggatccag catcgacgcc agtttttcca
     8401 ccattgtcag tcgcaggcta agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa
     8461 gctgcacacg cgaaagaagt tcctgcatca gtcgttcacg aaactgctgc tgatgggctt
     8521 gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga taaaccgact
     8581 ccagcacgcg accggggccg tccagtaacg tcttggtcaa tatcgttctg ccgtgaaaag
     8641 tgtttgaata tcatcgcgta acagctgggc gtcggtgtaa atccagccgt gagtcatcac
     8701 agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt gacgcagagc
     8761 caggctttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact cagcgcaaaa
     8821 agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt tagccatttg
     8881 ttggcaataa agatcgactt ctgcgcttaa agctcgttgc aacacactgt aatcaaccgt
     8941 ctctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa
     9001 agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcatcct ggcgcgaaat
     9061 gacttcactg aacagctggc gagtgttgac gtggggaact actccgtgaa aaacaggaaa
     9121 atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt gctcaatggg
     9181 ccgaataaca tcggttaaca cccggcaaag ggtggattca agctcggcaa aacgcagcgt
     9241 aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc aggtacgaat
     9301 ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat tcagcgcatc
     9361 ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt catgtttaat
     9421 ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt cgacctgatg
     9481 gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa aatccaggta
     9541 ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgca gcagatcgca gagcatacaa
     9601 cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga acagcgaatc
     9661 ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat cggcatgacg
     9721 ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt gctctggcgc
     9781 tggtaactta ccgttgttgg ctaactcatg gcgcgctcga tttgccaagt agccccacat
     9841 cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg tcccggaaat
     9901 cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga acttattgac
     9961 cagcacatac agtggcaccg attgccccac cgccaaaatc gcctcacgga cctcttcatc
    10021 ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg cgcgtgccag
    10081 ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc cgggggtatc
    10141 cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa actccacttc
    10201 aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc
    10261 cttcgccagt cgcactaaat cattcaaact tttcagacaa tgaaaaatag gctgggcacc
    10321 cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg cacgcatatc
    10381 tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca ggcgctgttg
    10441 taattgttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta ccggttcctt
    10501 ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat tacgattagg
    10561 cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt tcatagtacc
    10621 gacaatcgcc agcaccattt ccagtcggga aattttactc aactcattat tcagcatcgc
    10681 gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc
    10741 gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag
    10801 gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa attcttcctt
    10861 aaagcaaatt ttgttacttt tatttagcca gattgttttt gagttctgtt tt
//
LOCUS       DAZPJP010000131        10899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPJP010000131 DAZPJP010000000
VERSION     DAZPJP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.131"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHU9J_003036"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHU9J_003036"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1906517.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHU9J_003037"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHU9J_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1906518.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHU9J_003038"
     CDS             complement(3743..4162)
                     /locus_tag="ACHU9J_003038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1906519.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHU9J_003039"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHU9J_003039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1906520.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHU9J_003040"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHU9J_003040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1906521.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHU9J_003041"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHU9J_003041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1906522.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHU9J_003042"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHU9J_003042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1906523.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHU9J_003043"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHU9J_003043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1906524.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHU9J_003044"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHU9J_003044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1906525.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHU9J_003045"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHU9J_003045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1906526.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHU9J_003046"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHU9J_003046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1906527.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2591 a         2986 c         2712 g         2610 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgca
//
LOCUS       DAZPJP010000132        10866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPJP010000132 DAZPJP010000000
VERSION     DAZPJP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.132"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..699)
                     /locus_tag="ACHU9J_003047"
     CDS             complement(<1..699)
                     /locus_tag="ACHU9J_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1906528.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGC"
     gene            955..1110
                     /gene="rseD"
                     /locus_tag="ACHU9J_003048"
     CDS             955..1110
                     /gene="rseD"
                     /locus_tag="ACHU9J_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1906529.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1107..1682
                     /gene="rpoE"
                     /locus_tag="ACHU9J_003049"
     CDS             1107..1682
                     /gene="rpoE"
                     /locus_tag="ACHU9J_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1906530.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1715..2365
                     /gene="rseA"
                     /locus_tag="ACHU9J_003050"
     CDS             1715..2365
                     /gene="rseA"
                     /locus_tag="ACHU9J_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1906531.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2365..3321
                     /gene="rseB"
                     /locus_tag="ACHU9J_003051"
     CDS             2365..3321
                     /gene="rseB"
                     /locus_tag="ACHU9J_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1906532.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3318..3797
                     /gene="rseC"
                     /locus_tag="ACHU9J_003052"
     CDS             3318..3797
                     /gene="rseC"
                     /locus_tag="ACHU9J_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1906533.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3995..5794
                     /gene="lepA"
                     /locus_tag="ACHU9J_003053"
     CDS             3995..5794
                     /gene="lepA"
                     /locus_tag="ACHU9J_003053"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1906534.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5810..6784
                     /gene="lepB"
                     /locus_tag="ACHU9J_003054"
     CDS             5810..6784
                     /gene="lepB"
                     /locus_tag="ACHU9J_003054"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1906535.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7057..7737
                     /gene="rnc"
                     /locus_tag="ACHU9J_003055"
     CDS             7057..7737
                     /gene="rnc"
                     /locus_tag="ACHU9J_003055"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1906536.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7734..8639
                     /gene="era"
                     /locus_tag="ACHU9J_003056"
     CDS             7734..8639
                     /gene="era"
                     /locus_tag="ACHU9J_003056"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1906537.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8651..9379
                     /gene="recO"
                     /locus_tag="ACHU9J_003057"
     CDS             8651..9379
                     /gene="recO"
                     /locus_tag="ACHU9J_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1906538.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9391..10122
                     /gene="pdxJ"
                     /locus_tag="ACHU9J_003058"
     CDS             9391..10122
                     /gene="pdxJ"
                     /locus_tag="ACHU9J_003058"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1906539.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10122..10502
                     /gene="acpS"
                     /locus_tag="ACHU9J_003059"
     CDS             10122..10502
                     /gene="acpS"
                     /locus_tag="ACHU9J_003059"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1906540.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10724..10804
                     /gene="shoB"
                     /locus_tag="ACHU9J_003060"
     CDS             10724..10804
                     /gene="shoB"
                     /locus_tag="ACHU9J_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1906541.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2731 a         2673 c         2890 g         2572 t
ORIGIN      
        1 acagcccgcg cgccacgcca tagcaatgcc atcgccagaa gaaatatccg gattggtggt
       61 gtactgatac accttcgacg caccgccggt tgccagtacc actgcttttg cgtggcaggt
      121 ttccaccgtt tctttatttc ggttccatac ccacgcgcct acaacccgtc gcgtgcccgg
      181 caggccaatt ttgtcagaga caatcagatc aactgcgttg ctgcgctcca gcacacgaat
      241 attcggatgg ttctgcgcct tgctcaccag cgtggattgt acttctctac cggtggcgtc
      301 ggcggcatga agaatacgac ggtgactatg tccaccttca cgggtcagat ggtagctttc
      361 ttcgccattc ggttgaacgt gggtatcaaa caaaaccccc tggtcaatta accattgcac
      421 acaggaacga gcattgctgg cgacaaattc aactgcatgg cgatcgcaaa taccagcccc
      481 ggcaatcaat gtgtcttcca catgcgagtc aatgctgtca gtttcatcaa acacggcggc
      541 aataccgccc tgggcataaa atgttgaacc ttccgttacc gggcctttac ttagaacgat
      601 gacctgatgc tggtcagcca ggcgtagcgc cagtgaaagt ccggctgcgc cgctaccaat
      661 aatcaacacg tcacatgaat gttcagggag agtattcatt ttctttgttt aatttactaa
      721 acattgtttg gtcagcatag catcatgttg tgcgaataaa cacctgctat tttaatattt
      781 gttacagtgg ctaaacacgt tgacgcaggg cggcgagaaa aagagaagtt actggctggt
      841 ggaggattag gtggtgaaat aaaaaggccg ttgggttact cttcaggcag ttaaatgggc
      901 atttctacac agataatgcg atgttcagat tctgtagact tataatgata gataatgatc
      961 cgtctacagc atgacaaaca aaaacagatg cgttacggaa ctttacaaaa acgagacact
     1021 ctaacccttt gcttgctcaa attgcagcta atggagtggc gtttcgatag cgcgtggaaa
     1081 tttggtttgg ggagacttta cctcggatga gcgagcagtt aacggaccag gtcctggttg
     1141 aacgggtcca gaagggagat cagaaagcct ttaacttact ggtagtgcgc tatcagcata
     1201 aagtggcgag tctggtttcc cgctatgtgc cgtcgggtga tgttcccgat gtggtacaag
     1261 aagcttttat taaagcctat cgtgcgctgg attcgttccg gggagatagc gctttttata
     1321 catggctgta tcggattgct gtaaatacag cgaaaaatta cctggttgct caggggcgtc
     1381 gtccaccttc cagtgatgtg gatgccattg aagctgaaaa cttcgaaagt ggcggcgcgt
     1441 tgaaagaaat ttcgaaccct gagaacttaa tgttgtcaga agaactgaga cagatagttt
     1501 tccgaactat tgagtccctc ccggaagatt tgcgcatggc aataaccttg cgggagctgg
     1561 atggcctgag ctatgaagag atagccgcta tcatggattg tccggtaggt acggtgcgtt
     1621 cacgtatctt ccgagcgagg gaagctattg ataacaaagt tcaaccgctt atcaggcgtt
     1681 gacgatagcg ggatactgga taagggtatt aggcatgcag aaagaacaac tttccgcttt
     1741 aatggatggc gaaacgctgg atagtgagct gcttaacgaa ctggctcata acccagaaat
     1801 gcagaaaacc tgggaaagct atcacttaat ccgtgattca atgcggggtg atactcccga
     1861 ggtgctccat ttcgatatct cttcacgcgt gatggccgcc attgaagaag agccagtacg
     1921 tcaaccggcg acattgatcc cggaagccca gcctgcgccg catcaatggc acaaaatgcc
     1981 attctggcag aaagtacgtc cgtgggcggc acagcttacc caaatgggcg tagcagcatg
     2041 cgtatcgctt gcagttatcg ttggcgtcca gcactataat ggacaatctg aaacgtccca
     2101 gcagcccgaa acgccggtat ttaatacact gccgatgatg ggtaaagcca gcccggtaag
     2161 cctgggagta ccttctgaag cgaccgcaaa caatggtcaa cagcagcagg tacaggagca
     2221 gcgtcgtcgc attaatgcaa tgttgcagga ttacgaactg caacgccgac tccactctga
     2281 acagcttcag tttgagcagg cgcaaaccca gcaagccgct gtacaggtgc caggacttca
     2341 aactttagga acgcaatcgc agtaatgaag caactttggt ttgccatgtc attagtgaca
     2401 ggtagcctgt tattctctgc taacgcctcg gccactcccg cgtccggggc gttattacag
     2461 cagatgaacc tggccagtca gtcactgaat tacgagctgt cattcatcag catcaataaa
     2521 cagggtgttg agtctctgcg ttatcgacat gcacgcctcg ataaccgtcc tcttgcacaa
     2581 ttgttgcaaa tggatggccc gcgccgggaa gtggtacagc gcggcaatga aatcagctat
     2641 tttgaaccgg gacttgaacc gttcacgctt aatggcgatt acattgttga ttctctgcca
     2701 tcgcttatct ataccgattt caaacgcctt tctccttact acgactttat ctccgtcggg
     2761 cgcacgcgta ttgctgatcg tctttgcgaa gtcattcgcg tggttgcccg agatggtaca
     2821 cgctacagct acatcgtgtg gatggacact gaatcgaaat taccgatgag ggttgatctc
     2881 cttgatcgcg atggtgaaac gctggaacaa tttcgcgtga tttcttttaa cgtcaatcag
     2941 gatatcagca gcagtatgca gacgctggcg aaggcaaatt tgccgccgtt gctttctgtt
     3001 cctgtaggtg aaaaagctaa attcagctgg acgccaacct ggttgccaca gggttttagc
     3061 gaagtttcca gtagtcgacg tccgctaccg acgatggaca acatgcctat cgaatcacgt
     3121 ctctattccg acggattatt cagcttctcg gtaaacgtta accgcgctac gccatcgagc
     3181 accgatcaga tgttgcgcac cggacgcaga accgtcagta caagcgtacg tgataacgcc
     3241 gaaatcacca ttgtcggtga actgccgccg caaacggcga aacgcattgc cgagaatatt
     3301 aagttcgggg cagcgcaatg atcaaagagt gggctaccgt cgtctcctgg caaaacgggc
     3361 aggcgctggt cagttgtgat gttaaagcct catgcagcag ctgcgcttca cgcgccggtt
     3421 gcggtagccg cgtgttgaat aaacttggcc cgcaaaccac gcataccatt gtcgtaccct
     3481 gtgatgaacc gttagtgccg gggcaaaaag tggaattggg gatcgccgaa ggcagcctgc
     3541 ttagctccgc attactggtt tatatgtcgc cgctggtggg attattcctt atcgcttcgc
     3601 tatttcagct actctttgct tctgacgttg cagcattatg tggtgcgatc cttggcggta
     3661 ttggcgggtt tctgattgcg cgcggttact cgcgtaagtt tgctgcccgg gcagaatggc
     3721 aaccgatcat attaagcgtg gcattgccgc ctgaccttgt gcgatttgaa acatcttcgg
     3781 aagacgcgag ccagtgattt cactggcttt gttgcatttt gcataacaaa tgcgaggacg
     3841 tttccagata ccacaatcca tacggtatga acatttcctc gcctcaatgt tgtagtgtag
     3901 aatgcggcgt ttctattaat acagacgtta agctcagaac agcgacttct aaaagcctgg
     3961 ttaaccgggc attaaggcac aataatcata ctttatgaag aatatacgta acttttcgat
     4021 catagctcac attgaccacg gtaaatcgac gctgtctgac cgtattatcc agatctgcgg
     4081 tggcctgtct gaccgtgaaa tggaggcgca ggttctcgat tccatggatc ttgagcgtga
     4141 gcgtggcatt accatcaaag cgcaaagcgt gacgctggac tacaaagcgt ctgacggcga
     4201 aacctatcag cttaacttta tcgacacccc gggccacgta gacttctcct atgaagtttc
     4261 ccgttcgctg gctgcctgtg aaggtgcatt gctggtggtc gacgccgggc agggcgtgga
     4321 agcgcaaacc ctggcaaact gctacaccgc catggaaatg gatctcgaag tggtgccagt
     4381 actgaacaag attgacctgc cggcagccga tcctgaacgc gtggcggaag aaattgaaga
     4441 tatcgtcggc atcgacgcca ctgacgcggt gcgctgttca gcgaaaaccg gcgttggcgt
     4501 gcaggacgtt ctcgaacgtc tggtgcgcga cattccgccg ccggaaggcg atccggaagg
     4561 cccgttgcag gcactaatta tcgactcatg gttcgacaac tacctgggcg ttgtttcact
     4621 tatccgtatt aaaaacggca ccctgcgtaa gggcgacaaa gtgaaagtca tgagtaccgg
     4681 gcagacctat aacgccgacc gtctgggcat cttcacgccg aaacaggttg accgcactga
     4741 actgaaatgt ggcgaagtag gctggctggt atgtgcgatt aaagatatcc acggcgctcc
     4801 agtcggcgat accttaacgc tggcgcgtaa tccggcagaa aaggcgctgc ctggctttaa
     4861 gaaagtcaaa ccgcaggtat acgccggtct gttcccggta agttccgacg actatgaagc
     4921 cttccgtgac gcgctgggta aactcagcct gaatgatgcc tcactgttct atgagccgga
     4981 aagctccagc gcgctgggct ttggtttccg ctgcggcttc cttggcctgc tgcacatgga
     5041 gatcatccag gaacgtctgg aacgtgaata cgatctggat ctgatcacca ctgcgccgac
     5101 cgtagtgtat gaagttgaaa ccacgtcaag ggaagttatc tacgtcgaca gcccatccaa
     5161 gctgcctgcg gtaaataaca tttacgaact gcgcgagccg attgcagagt gtcacatgct
     5221 gctgccgcag gcatatctcg gcaacgttat tacgctgtgc gtagaaaaac gcggcgtgca
     5281 gaccaatatg gtttaccacg gtaatcaggt ggcgctgacg tacgagatcc cgatggcgga
     5341 agtggtgctc gacttcttcg atcgcctgaa atctacctcg cgtggttatg cgtctctgga
     5401 ttacaacttc aaacgcttcc aggcgtccga catggtacgt gtagacgtat taatcaacgg
     5461 tgaacgtgtt gatgcgttgg cgctgatcac ccaccgtgat aattcgcaaa accgcggtcg
     5521 cgagttggtg gagaagatga aagatctgat cccacgccag cagtttgata tcgccattca
     5581 ggcagcgatt ggtacgcaca ttattgcgcg atccaccgtg aaacagctgc gtaaaaacgt
     5641 actggctaaa tgttatggcg gcgatatcag ccgtaagaaa aagctgctgc agaagcagaa
     5701 agaaggtaag aaacgcatga agcagatcgg taacgtcgag ctgccgcagg aagcgttcct
     5761 cgccattctg cacgtcggca aagacaacaa ataaccctta ggagttggca tggcgaatat
     5821 gtttgccctg attctggtga ttgccacact ggtgacgggc attttatggt gcgtggataa
     5881 attctttttc gcacctaaac ggcgggaacg tcaggcagcg gcgcaggcgg ctgccggtga
     5941 ctcactggat aaagcaacgt tgaaaaaggt tgcaccgaag cctggctggc tggaaaccgg
     6001 agcttctgtt tttccggtgc tggctatcgt attgattgta cgttcgttta tttatgaacc
     6061 gttccagatc ccgtcaggtt cgatgatgcc gactctgtta atcggtgatt ttattctggt
     6121 agagaagttt gcttatggca ttaaagatcc tatctaccag aaaacgctga tcgaaaccgg
     6181 tcatccgaaa cgcggcgata tcgtggtctt taaatatccg gaagatccaa agcttgatta
     6241 catcaagcgc gcggtgggtt taccgggcga taaagtcact tacgatccgg tctcaaaaga
     6301 gctgacgatt caaccgggat gcagttccgg ccaggcgtgt gaaaacgcgc tgccggtcac
     6361 ctactcaaac gtggaaccga gcgatttcgt tcagaccttc tcacgccgta atggtgggga
     6421 agcgaccagc ggattctttg aagtgccgaa aaacgaaacc aaagaaaatg gaattcgtct
     6481 ttccgagcgt aaagagacac tgggtgatgt gacgcaccga attctgacag tgccgattgc
     6541 gcaggaccag gtggggatgt attaccagca gccagggcaa caactggcaa cctggattgt
     6601 tccgccggga caatacttca tgatgggcga caaccgcgac aacagtgcgg acagccgtta
     6661 ctggggcttt gtgcctgaag cgaatctggt cggtcgggca acggctatct ggatgagttt
     6721 cgataagcaa gaaggcgaat ggccgacagg tgtgcgctta agtcgcattg gcggcatcca
     6781 ttaatagccc atcttcgttc acgttgtcgc cgttatggcg acaacgtgaa ttatttatga
     6841 gataaatctc ccgtggctaa cgacatcccc cgtcgttgtg tatagaatat tcccccgaag
     6901 tttaaggttg gcacctccag gttgccacgg cacacgaaac agcgttggtc cccatatacc
     6961 ggtaaactga aactgcagcg aagcagttag cagagccatg tatatcaggt ctgtttcgtg
     7021 tgctgaattg ttgacgcatt tatttattgg tatcgcatga accccatcgt aattaatcgg
     7081 cttcaacgga agctgggcta cacttttaat catcaggaac tgttgcagca ggcattaact
     7141 catcgtagtg ccagcagcaa acataacgag cgtttagaat ttttaggcga ctctattctg
     7201 agctacgtta tcgccaatgc gctttatcac cgtttccctc gtgtggatga aggcgatatg
     7261 agccggatgc gcgccacgct ggtccgtggc aatacgctgg cggaactggc gcgcgaattt
     7321 gagttaggcg agtgcttacg tttagggcca ggtgaactta aaagcggtgg atttcgtcgt
     7381 gagtcaattc tcgccgatac cgtcgaagca ttaattggtg gcgtattcct cgacagtgat
     7441 attcaaaccg tcgagaaatt aatcctcaac tggtatcaaa ctcgtttgga cgaaattagc
     7501 ccaggcgata aacaaaaaga tccgaaaacg cgcttgcaag aatatttgca gggtcgccat
     7561 ctgccgctgc cgacttatct ggtagtccag gtacgtggcg aagcgcacga tcaggaattt
     7621 actatccact gccaggtcag cggcctgagt gaaccggtgg ttggcacagg ttcaagccgt
     7681 cgtaaggctg agcaggctgc cgccgaacag gcgttgaaaa aactggagct ggaatgagca
     7741 tcgataaaag ttactgcgga tttattgcca tcgtcggacg tccgaacgtt ggcaaatcca
     7801 cattgttgaa caaactgctg gggcagaaga tctccatcac ctcccgcaaa gcgcaaacaa
     7861 ctcgtcaccg cattgtgggg atccatactg aaggcgcgta tcaggcgatc tacgttgata
     7921 cccctggcct gcatatggaa gaaaaacgcg ctattaaccg cctgatgaac aaagcggcga
     7981 gcagctctat tggcgacgtt gagctggtga tttttgtcgt tgaaggcacc cgctggacgc
     8041 cggacgacga aatggtgctc aacaaactgc gcgatggcaa agcgccggtg atcctcgcgg
     8101 tgaacaaagt ggataacgtg caggaaaaag ccgatctgct gccgcactta cagttcctgg
     8161 caagtcagat gaacttcctc gatatcgtgc cgatctctgc tgaaaccggg ctgaatgtcg
     8221 acactatcgc agcaatcgtg cgtaagcatc tgcctgaagc gacccatcac ttcccggaag
     8281 attacatcac cgatcgctca cagcgtttta tggcgtctga aatcatccgc gaaaaactga
     8341 tgcgtttcct cggcgctgaa ctgccgtact ccgtgaccgt ggagatcgaa cgtttcgtct
     8401 ctaacgaacg cggtggttat gacatcaatg gtttgattct cgttgagcgt gaagggcaga
     8461 agaagatggt tattggcaac aaaggggcca agatcaaaac catcgggatt gaagcgcgta
     8521 aagacatgca ggaaatgttc gaagcgcctg tccaccttga actgtgggta aaagtgaaat
     8581 ccggttgggc cgacgacgaa cgcgcactgc gcagtctcgg ttacgttgac gatctttaag
     8641 agtcactccg atggaaggct ggcagcgcgc atttgtcctg catagtcgcc cgtggagcga
     8701 aaccagcctg atgctggacg tcttcacgga ggaatcgggt cgcgtgcgtc tggttgccaa
     8761 aggcgcacgc tctaaacgct ctactctgaa aggtgcatta cagcctttca cccctctctt
     8821 gctacgtttc ggcgggcgtg gcgaagtcaa aacgctgcgc agtgctgaag ctgtctcgct
     8881 ggcgctgcca ttaagcggta tcacgcttta cagcggtctg tacatcaacg aacttctctc
     8941 ccgcgtactg gaatacgaga cgcgcttctc tgaactcttt ttcgattact tgcactgcat
     9001 tcagtctctt gcaggtgcca ctggtacgcc agaacccgcg ctgcgccgct ttgaactggc
     9061 acttctcggg catctgggct atggcgtcaa ttttacccat tgcgcgggta gcggcgagcc
     9121 ggtagatgac accatgacgt atcgttatcg cgaagaaaaa gggtttatcg caagcgtcgt
     9181 tatcgacaat aaaacgttca ccggaaggca gttaaaagcg ttaaacgcgc gggaatttcc
     9241 tgacgcagac acactacgcg ccgcgaaacg ctttacccgc atggcgctta agccgtatct
     9301 tggcggtaaa ccattaaaga gcagggaact gttccggcag tttatgccta agcgaacggt
     9361 gaaaacacat tatgaatgat gaggattgtc atggctgaat tactgttagg cgtcaacatt
     9421 gaccatattg caacgctgcg taacgcacgc ggtactgctt acccggatcc ggtgcaggcg
     9481 gcgtttattg ccgagcaggc gggagcggac ggcattaccg tgcatttacg tgaagatcgc
     9541 cgccacatta ctgaccgcga tgtgcgcatc ctgcggcaga cgctggatac ccgaatgaat
     9601 ctggagatgg cagtgaccga agagatgctg gcgatcgccg ttgagacgaa gccacatttt
     9661 tgctgcctgg taccggaaaa gcgtcaggaa gtaacaaccg aaggcggcct ggacgttgca
     9721 gggcagcgtg aaaaaatgcg cgatgcctgc aaacgtctga cagatgccgg gattcagatt
     9781 tctctcttta tcgacgccga tgaagagcag atcaaagctg cggcagaggt tggcgcaccg
     9841 tttatcgaga tccacaccgg ttgctatgct gatgccaaaa ctgacgccga acaggcgcaa
     9901 gagctggtgc gtatcgccaa agccgcgacc tttgccgcta gtctcggtct gaaagttaac
     9961 gccggacacg gtctgaccta tcacaacgtg aaagccattg ccgccatccc tgagatgcat
    10021 gaactgaata tcggtcatgc cattattggc cgtgcagtga tgaccggact gaaagatgcg
    10081 gtggcagaaa tgaagcgtct gatgctggaa gcgcgtggct aatggcaata ttaggtttag
    10141 gcacggatat cgtagagatc gctcgcatag aagcagtgat cgcccgatcc ggtgagcgcc
    10201 tggcacgccg cgtattaagc gataacgaat gggcaatctg gaaaacgcac caccagccgg
    10261 tgcgttttct ggcgaagcgt tttgctgtga aagaagccgc agcaaaagcg tttggcaccg
    10321 ggatccgcaa tggtctggcg tttaatcaat ttgaagtatt caatgatgag ctcggcaaac
    10381 cacggctacg gctatggggt gaggcattaa aactggcgga aaagctcggc gttgtaaata
    10441 tgcatgtaac gctggcagat gaacgccact atgcttgtgc cacggtaatt attgaaagtt
    10501 aaaataatac cgttctcttt aaaaagtaaa acccccgaac tggtaaggaa cgggggtttt
    10561 actttttaac tttaattttg tgcagcatgc accctcaaca acagcgatta tggatcagtg
    10621 tacttatctt ttacaagcaa cttgcaatct ttagcataaa aactcaagcc tttacgaaga
    10681 aagcaatact gaacggctgc attccttgtt taaggaaaaa cgaatgactg attgccgata
    10741 cctgattaaa cgggtcatca aaatcatcat tgctgtttta cagctgatcc ttctgttctt
    10801 ataacacaag gaaacgtact taaggtgcgt ccggtgaacc aatcggacgc gcctttaata
    10861 actata
//
LOCUS       DAZPJP010000133        10835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPJP010000133 DAZPJP010000000
VERSION     DAZPJP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.133"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHU9J_003061"
     CDS             complement(<1..279)
                     /locus_tag="ACHU9J_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1906542.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHU9J_003062"
     CDS             complement(446..1186)
                     /locus_tag="ACHU9J_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1906543.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHU9J_003063"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHU9J_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1906544.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHU9J_003064"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHU9J_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1906545.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHU9J_003065"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHU9J_003065"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1906546.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHU9J_003066"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHU9J_003066"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1906547.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHU9J_003067"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHU9J_003067"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1906548.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHU9J_003068"
     CDS             7002..7433
                     /locus_tag="ACHU9J_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1906549.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHU9J_003069"
     CDS             7456..8034
                     /locus_tag="ACHU9J_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1906550.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHU9J_003070"
     CDS             8035..8742
                     /locus_tag="ACHU9J_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1906551.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHU9J_003071"
     CDS             8730..9407
                     /locus_tag="ACHU9J_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1906552.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHU9J_003072"
     CDS             9401..10078
                     /locus_tag="ACHU9J_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1906553.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHU9J_003073"
     CDS             complement(10050..10763)
                     /locus_tag="ACHU9J_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1906554.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10835)
                     /locus_tag="ACHU9J_003074"
     CDS             complement(10760..>10835)
                     /locus_tag="ACHU9J_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1906555.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2880 c         2699 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tgtttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgcc
//
LOCUS       DAZPJP010000134        10777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPJP010000134 DAZPJP010000000
VERSION     DAZPJP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.134"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHU9J_003075"
     CDS             <1..656
                     /locus_tag="ACHU9J_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1906556.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHU9J_003076"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHU9J_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1906557.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHU9J_003077"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHU9J_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1906558.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHU9J_003078"
     CDS             2258..2494
                     /locus_tag="ACHU9J_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1906559.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHU9J_003079"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHU9J_003079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
     gene            complement(2654..2740)
                     /locus_tag="ACHU9J_003080"
     tRNA            complement(2654..2740)
                     /locus_tag="ACHU9J_003080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2704..2706),aa:Leu,seq:cag)
     gene            complement(2769..2855)
                     /locus_tag="ACHU9J_003081"
     tRNA            complement(2769..2855)
                     /locus_tag="ACHU9J_003081"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2819..2821),aa:Leu,seq:cag)
     gene            complement(3123..4154)
                     /gene="rsmC"
                     /locus_tag="ACHU9J_003082"
     CDS             complement(3123..4154)
                     /gene="rsmC"
                     /locus_tag="ACHU9J_003082"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1906560.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4257..4670
                     /gene="holD"
                     /locus_tag="ACHU9J_003083"
     CDS             4257..4670
                     /gene="holD"
                     /locus_tag="ACHU9J_003083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1906561.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4639..5085
                     /gene="rimI"
                     /locus_tag="ACHU9J_003084"
     CDS             4639..5085
                     /gene="rimI"
                     /locus_tag="ACHU9J_003084"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1906562.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5100..5777
                     /gene="yjjG"
                     /locus_tag="ACHU9J_003085"
     CDS             5100..5777
                     /gene="yjjG"
                     /locus_tag="ACHU9J_003085"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1906563.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5868..7457
                     /gene="prfC"
                     /locus_tag="ACHU9J_003086"
     CDS             5868..7457
                     /gene="prfC"
                     /locus_tag="ACHU9J_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1906564.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7850..8455
                     /gene="osmY"
                     /locus_tag="ACHU9J_003087"
     CDS             7850..8455
                     /gene="osmY"
                     /locus_tag="ACHU9J_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1906565.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8582..8743
                     /locus_tag="ACHU9J_003088"
     CDS             8582..8743
                     /locus_tag="ACHU9J_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1906566.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8865..9938
                     /locus_tag="ACHU9J_003089"
     CDS             8865..9938
                     /locus_tag="ACHU9J_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1906567.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9935..10717
                     /locus_tag="ACHU9J_003090"
     CDS             9935..10717
                     /locus_tag="ACHU9J_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1906568.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2621 a         2701 c         2877 g         2578 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca cagtcatctt actttttttg
     2641 atatcgcctc gtttggtgcg aggggggaga cttgaacccc cacgtccgta agaacactaa
     2701 cacctgaagc tagcgcgtct accaattccg ccaccttcgc ccagtgcgag caatatcaac
     2761 gtggtttttg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
     2821 gaagctagcg cgtctaccaa ttccgccacc ttcgcatacc atcaattctt aaaaagaatt
     2881 gctaccacgg aggcgcattc tagtggtttt cagcttttcg tcaatagtta attatcgccg
     2941 gaggtgtaat tgctggaaaa atgtccatca ggaaactagc gtgcaggttt ggtaggcatg
     3001 cgggggcaga tgccagatgc gacgctggcg cgtcttatct ggcctacgaa gggctaacgt
     3061 gcaggttttg taggtcggat aaggcgttca cgccgcatcc gacacggtat tcggcgagat
     3121 aattaacctt tcttcgcctg gcgggtcata atggcgcgat acaccttgaa acgcccggtt
     3181 tgcgcaatca cttcatggaa gccaaatgtc tcatccagca cgtccgggta aggcaggaag
     3241 gcgttcgcta caattcgcag ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc
     3301 agcgtttgcg ccgcatccag gctggtttgc atcccatcgt ggaacggcgg gttggagatg
     3361 atcatatcaa aacgaccttt cacctcggaa aagacgttgc tggcaaagac ttcaccttca
     3421 acacagttgg ccgcaagtgt tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac
     3481 aaggtgagac gaattttcgg cgaatggcgc gcaaaggcaa ctgaaagcac ccccgcgcca
     3541 cagccgacat ccagcacttt acctttcgtg tgcggagtta acgtcgagag cagcaactgg
     3601 ctaccgacat ccagaccgtc gcggctaaac acgccaggca gcgttttgac cgtcaggcca
     3661 tcgacgctgt attcgcccca gaatttatcg gcatcaaata ccggctgttt ttccagacga
     3721 ccaaaataga ggccacagcg acgagcgctg tcgactttat tcaacggcgc ataatctgcc
     3781 agcatctgct cggcgctgcg cacgccgctg cggttctcgc caacgacaaa aatatctgtc
     3841 cccactggca gcagagaaag taaattcatc aactggaact gggcttccgg tttgttcttc
     3901 ggccagtagt aaatcagcgt atcgcaatct gcgacgtcat ccaccgtggc gaccagactg
     3961 aaacgggcgt tatcccccat ctggcggctt aatacctgcc agtggtggaa ttgctgggta
     4021 tgagcacggc tggccgcggt atctaaacgc gcgggcaggt catcctgtaa gtctccggca
     4081 aacagaatac ggctttgttc gaaatcatca ctgtgacgca gcaagacttc acttgccggg
     4141 gtaaatgcag acatggaatg ctcctcaatt gatactggcg gcgattatag ccatatgttg
     4201 gcgcggtatc gacgaatttg ctatatttgc gcccctgaca acaggagcga ttcgctatga
     4261 catcccgacg agactggcag ttacagcaac tgggcattac ccagtggtcg ctgcgtcgcc
     4321 ctggcgcgtt gcagggggag attgccattg cgatcccggc acacgtccgt ctggtgatgg
     4381 tggcaaacga tcttcccgcc ctgaccgatc ctttagtgag cgatgttctg cgcgcattaa
     4441 ccgtcagccc cgatcaagtg ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag
     4501 gcagtcgctg caacagctgg cggttgggta ctgacgaacc gctatcactg gaaggcgctc
     4561 aggtggcatc accggcgctc accgaattac gggcaaaccc aacggcacgc gccgcgttat
     4621 ggcaacagat ttgcacatat gaacacgatt tcttccctcg aaacgactga tttaccagcg
     4681 gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc
     4741 aaccagggcg agcgttatct caactttcag ttaacgcaaa acggcaaaat ggcggcgttt
     4801 gcgattacgc aagtggtgct ggatgaagct acattgttca atattgcggt cgatcctgac
     4861 tatcagcgtc agggattggg aagggtgctg ctggaacatc tgatcgacga actggaaaaa
     4921 cgcggcgtgg cgacactatg gctggaagtc cgtgcctcaa atgctgccgc cattgccctg
     4981 tacgaaagtt taggctttaa cgaggcgacg attcgccgca attactaccc caccacggac
     5041 ggtcgcgaag acgccatcat catggcgttg ccaatcagta tgtaagacaa ggtggaataa
     5101 tgaagtggga ctggattttc tttgatgccg atgaaacgct gtttaccttt gactcattca
     5161 ccggcctgca gcggatgttt cttgattaca gcgtcacctt taccgctgaa gattttcagg
     5221 actatcaggc cgttaacaag ccactgtggg tggattatca aaacggcgcg atcacttcat
     5281 tacagcttca gcacgggcgt tttgagagct gggccgaacg gctgaaagtt gaagcaggct
     5341 tgcttaacga tgcctttatt aatgcgatgg cggaaatctg cacgccgctg ccgggcgcgg
     5401 tttctctgct taacgccatt cgtggcaacg ccaaaatcgg catcatcacc aacggcttta
     5461 gcgccttgca gcaagtgcgt ctggaacgca cgggcctgcg tgattacttc gatttgctgg
     5521 tgatttccga agaagttggc gttgccaaac cgaataagaa aattttcgat tatgcgctgg
     5581 aacaggcggg caatcctgac cgttcacgcg tgctgatggt tggcgacact gccgagtccg
     5641 atattctcgg tggcatcaac gccgggcttg cgacctgctg gctgaatgcg cacaatcgcg
     5701 agcaaccaga aggcatcgcg cccacctgga ccgtttcttc gttgcacgaa ctggagcagc
     5761 tcctgtgtaa acactgattg cctccccccc gttgatgggt aaaatagccg caatttttcg
     5821 ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga aagaattatg acgttgtctc
     5881 cttatttgca agaggtggcg aagcgccgca cttttgccat tatttctcac ccggatgccg
     5941 gtaagactac catcaccgag aaggtgctgc tgttcggaca ggccattcag accgccggta
     6001 cagtaaaagg ccgtggttcc aaccagcacg ctaagtcgga ctggatggag atggaaaagc
     6061 agcgtgggat ctccattact acgtctgtga tgcagtttcc gtatcacgat tgcctggtta
     6121 acctgctcga caccccgggg cacgaagact tctcggaaga tacctatcgt accctgacgg
     6181 cggtggactg ctgcctgatg gttatcgacg ctgcaaaagg tgttgaagat cgtacccgta
     6241 agctgatgga agttacccgt ctgcgcgaca cgccgatcct cacctttatg aacaaacttg
     6301 accgtgatat ccgcgacccg atggaactgc tcgatgaagt tgagaacgag ctgaaaatcg
     6361 gctgcgcacc gatcacctgg ccgattggct gcggcaagct gtttaaaggc gtttaccacc
     6421 tttataaaga cgaaacctat ctctatcaga gcggtaaagg ccacacgatt caggaagtcc
     6481 gtattgttaa agggctgaat aacccggatc tcgacgctgc ggttggtgaa gatctggcac
     6541 agcagctacg tgacgaactg gaactggtga aaggcgcgtc taacgagttc gacaaagaat
     6601 tgttccttgc gggcgaaatc actccggtat tcttcggtac tgcgctgggt aacttcggcg
     6661 ttgatcatat gctggatggc ctggtggagt gggctcctgc gccgatgccg cgtcagactg
     6721 atacccgtac cgtagaggcg agcgaagaca aatttaccgg cttcgtattt aaaattcagg
     6781 ccaacatgga cccgaaacac cgcgaccgcg tggcgtttat gcgcgtagta tccggtaaat
     6841 atgaaaaagg catgaagctg cgccaggtgc gtactgcgaa agatgtggtt atctccgacg
     6901 cgctgacctt tatggcgggc gaccgttcgc acgttgaaga agcgtatccg ggcgatatcc
     6961 tcggcctgca caaccacggc actattcaga tcggcgacac ctttacccag ggtgagatga
     7021 tgaagttcac cggtattccg aacttcgcac cagaactgtt ccgtcgtatc cgcctgaaag
     7081 atccgctgaa gcaaaaacag ctgctcaaag ggctggtaca gctttccgaa gagggcgcgg
     7141 tgcaggtgtt ccgtccgatc tccaacaacg atttgatcgt tggtgctgtt ggtgtgctgc
     7201 agtttgatgt ggtggtatcg cgcctgaaga gcgaatacag cgttgaagca gtatatgaat
     7261 cagtcaacgt tgccactgcc cgctgggtag aatgtgcgga cgcgaagaaa ttcgaagagt
     7321 tcaagcgtaa gaacgaaagc caactggcgc ttgatggcgg cgataacctc gcttacatcg
     7381 ctaccagcat ggtcaacctg cgcctggcgc aggaacgtta tcccgacgtt cagttccacc
     7441 agacccgcga gcattaattc ttgcctccag ggcgcggtag ccgctgcgcc ctgtcaattt
     7501 cccttcctta ttagccgctt acggaatgtt cttaaaacat tcacttttgc ttatgttttc
     7561 gctgatatcc cgagcggttt caaaattgtg atctatattt aacaaagtaa tgacatttct
     7621 gacggcgtta aataccgttc aatgcgtaga tatcagtatc taaagccgtc gattgtcatt
     7681 ctaccgatat taataactga ttcagaggct gtaatggtcg ttattcatca cccatcgctt
     7741 ttgtgatggc gaccattgac ttctgtagag ggtgaagtct ctccctattc agcaatgcaa
     7801 cctcgtgttg ccaggctcaa attacgagca aacatacagg aataaatcga tgactatgac
     7861 aagactgaag atttcgaaaa ctctgctggc tgtaatgttg acctctgccg tcgcgaccgg
     7921 ctctgcctac gcggaaaaca acgcacagac taccaatgaa agcgcagggc aaaaagtcga
     7981 tagctctatg aataaagtcg gtaatttcat ggatgacagc gccatcaccg cgaaagtgaa
     8041 ggcggcgctg gtggatcatg acaacatcaa gagcaccgat atctctgtca aaaccgatca
     8101 aaaagtcgtg accctgagcg gtttcgttga aagccaggct caggccgaag aggcagtgaa
     8161 agtggcgaaa ggcgttgaag gggtgacctc tgtcagcgac aaactgcacg ttcgcgacgc
     8221 taaagaaggc tctgtgaagg gctacgcggg tgacaccgcc accaccagtg aaatcaaagc
     8281 caaactgctg gcggacgata tcgtcccttc ccgtcatgtg aaagttgaaa ccaccgacgg
     8341 cgtggttcag ctctccggta ccgtcgattc ccaggcacag agcgaccgtg ctgaaagtat
     8401 cgccaaagcg gtagatggtg tgaaaagcgt taaaaatgat ctgaaaacta agtaattcgt
     8461 cgtaattcgt cctcccgaac tttgtcggga ggcgcgatgt gcaccacact aaaaatatcg
     8521 cattgagtga ttttcacgct catatcaagc ggtgacatta actatggtaa aggagacgct
     8581 tatgtttcgt tggggcatca tatttctggt tatcgcgtta atcgccgccg cacttgggtt
     8641 tggtggtctg gccggtaccg ctgcaggcgc agctaaaatt gtctttgtcg tcgggattat
     8701 tctgttcctg gtgagtttgt tcatgggccg aaaacgaccc tagatttcag accattaaga
     8761 aaattttatc caaagccagt ccagcggact ggcttttgcg gttttagcga ataataaatt
     8821 gcgttacttt gtcattcttt cacaacaaaa acaggaaggc agaggtgggg cagcgaatac
     8881 ctgtaacgct tggtaatatt gcgccgttgt cgctaaggcc gttccagcct ggacgaatag
     8941 ctctggtgtg cgaaggcggc ggacagcgtg gaattttcac ggctggcgtg ctggatgagt
     9001 ttatgcgcgc gcagtttaat cctttcgatc tttatctcgg cacatctgcc ggggcgcaga
     9061 acctctcggc gtttatctgc aatcagcccg gttacgcgcg caaagtcatc atgcgctata
     9121 ccacaaaacg cgaatttttc gatccattgc gctttgtccg tggaggaaat cttatcgatc
     9181 tcgactggct ggtggaggcc accgcaagcc agatgccgtt gcaaatggat accgccgcgc
     9241 ggttgtttga cagcggcaaa tcgttttata tgtgcgcctg tcgtcaggat gactacgcgc
     9301 ctaattactt tttaccaacc aaacaaaact ggctggatgt gattcgcgcc tccagtgcga
     9361 tacctggctt ttatcgtagc ggagtgtcgc tggaaggcat taactacctg gatggcggga
     9421 tcagtgatgc gattccggtt aaagaggcgg caaggcaggg cgctaaaacg ttggtcgtca
     9481 ttcgcactgt gccgtcacaa atgtactaca cgccgcagtg gttcaaacgc atggaacgct
     9541 ggctgggtga cagtagcctg cagccgctgg tcaatctggt gcagcatcat gaaaccagct
     9601 atcgtgacat tcagcaattt attgagaaac caccgggcaa gctgcggata ttcgaaattt
     9661 atccgccgaa gccattacat agtatcgcgc ttggcagtcg gattccggcg ctgcgtgaag
     9721 actataaact tgggcgttta tgcggtcgtt atttcctcgc cacggttggc aagctattaa
     9781 ctgaaaaagc gccgcttacc cgccatctgg tgccagtggt gacgccggaa tcgatcgtta
     9841 ttccgcctgc gccagtcgcc aacgatacgc tggttgccga agtgagcgat gctccgcagg
     9901 cgaacgaccc gacatttaac aatgaggatc tggcttgatt tgccgtttta tcgataccca
     9961 ctgccatttt gatttcccgc cgtttagtgg cgatgaagag gccagcctgc aacgcgcggc
    10021 acaagcgggc gtaggcaaga tcattgttcc ggcgacagag gcggaaaatt ttgcccgcgt
    10081 gcaggcgtta gcggaaaaat atcaaccgct gtatgccgca ttggggttgc atccggggat
    10141 gttggaaaaa catagcgatg tgtctcttga tcagctacag caggcgctgg aaaggcgtcc
    10201 ggcgaaggtg gtggcggtgg gggagatcgg tctggatctc ttcggcgacg atccgcaatt
    10261 tgagaggcag cagtggttac tcgacgaaca gctgaaactg gcgaaacgct acgatctgcc
    10321 ggtgatcctt cattcccggc gtatacacga caaactggcg atgcatctta aacgtcacga
    10381 tttaccgtgc actggcgtgg ttcacggttt ttccggcagc ctgcaacagg ccgagcggtt
    10441 tgtacagctg ggctacaaaa tcggcgtagg cggtactatc acctatccac gcgccagtaa
    10501 aacccgcgat gtcatcgcaa aattaccgct ggcatcgttg ttgctggaaa ccgacgcgcc
    10561 ggatatgccg ctcaacggct ttcaggggca gcctaaccga ccggaacagg ctgtgcgggt
    10621 gtttgacgtg ctttgcgagt tgcgcccgga accagaagat gagattgccg aagtgttgct
    10681 taataatacg tatgcgttgt ttagcgttag tgggtagcct acccttcgta ggcagcatcc
    10741 ggcagtcggc gcataatgcc tgatgcgacg cttaacg
//
LOCUS       DAZPJP010000135        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPJP010000135 DAZPJP010000000
VERSION     DAZPJP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.135"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHU9J_003091"
     CDS             complement(<1..784)
                     /locus_tag="ACHU9J_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1906569.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHU9J_003092"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHU9J_003092"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1906570.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHU9J_003093"
     CDS             1860..2747
                     /locus_tag="ACHU9J_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1906571.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHU9J_003094"
     CDS             2780..3790
                     /locus_tag="ACHU9J_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1906572.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHU9J_003095"
     CDS             3860..5293
                     /locus_tag="ACHU9J_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1906573.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHU9J_003096"
     CDS             5392..6741
                     /locus_tag="ACHU9J_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1906574.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHU9J_003097"
     CDS             6731..7618
                     /locus_tag="ACHU9J_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1906575.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHU9J_003098"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHU9J_003098"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1906576.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHU9J_003099"
     CDS             8352..9224
                     /locus_tag="ACHU9J_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1906577.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHU9J_003100"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHU9J_003100"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1906578.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHU9J_003101"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHU9J_003101"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1906579.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPJP010000136        10526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPJP010000136 DAZPJP010000000
VERSION     DAZPJP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.136"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHU9J_003102"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHU9J_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1906580.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHU9J_003103"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHU9J_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1906581.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHU9J_003104"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHU9J_003104"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1906582.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHU9J_003105"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHU9J_003105"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1906583.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHU9J_003106"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHU9J_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHU9J_003107"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHU9J_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1906584.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHU9J_003108"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHU9J_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1906585.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHU9J_003109"
     CDS             7164..7604
                     /locus_tag="ACHU9J_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1906586.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHU9J_003110"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHU9J_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1906587.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHU9J_003111"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHU9J_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1906588.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10526
                     /gene="rsxC"
                     /locus_tag="ACHU9J_003112"
     CDS             8833..>10526
                     /gene="rsxC"
                     /locus_tag="ACHU9J_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1906589.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2404 a         2720 c         2693 g         2709 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccg
//
LOCUS       DAZPJP010000137        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPJP010000137 DAZPJP010000000
VERSION     DAZPJP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.137"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHU9J_003113"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHU9J_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1906590.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHU9J_003114"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHU9J_003114"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1906591.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHU9J_003115"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHU9J_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR1906592.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHU9J_003116"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHU9J_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1906593.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHU9J_003117"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHU9J_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHU9J_003118"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHU9J_003118"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1906594.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHU9J_003119"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHU9J_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1906595.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHU9J_003120"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHU9J_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1906596.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHU9J_003121"
     CDS             complement(9457..10149)
                     /locus_tag="ACHU9J_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1906597.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2556 a         2522 c         2763 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZPJP010000138        10418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPJP010000138 DAZPJP010000000
VERSION     DAZPJP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.138"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            52..627
                     /gene="ssuE"
                     /locus_tag="ACHU9J_003122"
     CDS             52..627
                     /gene="ssuE"
                     /locus_tag="ACHU9J_003122"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1906598.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            620..1579
                     /gene="ssuA"
                     /locus_tag="ACHU9J_003123"
     CDS             620..1579
                     /gene="ssuA"
                     /locus_tag="ACHU9J_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1906599.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1576..2723
                     /gene="ssuD"
                     /locus_tag="ACHU9J_003124"
                     /pseudo
     CDS             1576..2723
                     /gene="ssuD"
                     /locus_tag="ACHU9J_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2735..3526
                     /gene="ssuC"
                     /locus_tag="ACHU9J_003125"
     CDS             2735..3526
                     /gene="ssuC"
                     /locus_tag="ACHU9J_003125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1906600.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3523..4290
                     /gene="ssuB"
                     /locus_tag="ACHU9J_003126"
     CDS             3523..4290
                     /gene="ssuB"
                     /locus_tag="ACHU9J_003126"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1906601.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4497..7109)
                     /gene="pepN"
                     /locus_tag="ACHU9J_003127"
     CDS             complement(4497..7109)
                     /gene="pepN"
                     /locus_tag="ACHU9J_003127"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1906602.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7375..8577
                     /gene="pncB"
                     /locus_tag="ACHU9J_003128"
     CDS             7375..8577
                     /gene="pncB"
                     /locus_tag="ACHU9J_003128"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1906603.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8747..10147
                     /gene="asnS"
                     /locus_tag="ACHU9J_003129"
     CDS             8747..10147
                     /gene="asnS"
                     /locus_tag="ACHU9J_003129"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1906604.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2342 a         2684 c         2821 g         2571 t
ORIGIN      
        1 aattgttatc aatgttaaca aaaaaagaac aattggttat aaggagagag tatgcgtgtc
       61 atcaccctgg cgggtagtcc tcgctttcct tctcgctcca gctccttgct ggaatatgcg
      121 cgggaaaaac taaatggcct ggatgtagag gtttatcact ggaatctgca aaacttcgcc
      181 ccggaagatc tgctttacgc tcgtttcgat agtccggcac tcaagacctt caccgaacag
      241 ctgcaacagg ctgatggact gatagtcgct acacctgtgt ataaagccgc ctattccggc
      301 gcgctgaaaa ccctgctcga cctgctgcca gagcgcgctt tgcaaggcaa agtggtgcta
      361 ccgctggcga cgggcggtac ggtggctcat ctgctggcgg tcgattatgc ccttaagcca
      421 gttttaagtg cgatgaaagc ccaggagctc ctgcacggcg tgtttgccga tgactcacaa
      481 gttattgatt accatcacaa accccagttc acgccaaatc tgcaaacccg tcttgatacc
      541 gcgctagaaa ctttctggca ggcattgcac cgccgcgatg ttcaggttcc tgaccttctg
      601 tctctgcgag gtaatgtcca tgcgtaacat cattaaactg gcgctggcgg gattgcttag
      661 cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa gcgttacgta taggctatca
      721 gaaaggcagt attggtatgg tattggcaaa aagccaccag ttactggaaa aacgctatcc
      781 gcaatcaaaa atctcctggg tggagttccc cgcgggtccg caaatgttgg aagcgttaaa
      841 cgttggcagt attgatctcg gcagtaccgg ggatattccg ccaatctttg cccaggctgc
      901 cggggctgat ttgatatatg tgggcgtcga gccaccgaag cccaaagccg aagtgattct
      961 ggtggcagaa aacagcccga tcaaaaccgt agccgatctt aaaggtcaca aagttgcctt
     1021 tcagaaaggt tccagttcac acaacctttt actgcgtgca ctgcgtcagg ccggacttaa
     1081 gtttactgac atccagccca cttatctgac gccagctgat gcccgcgccg cgttccagca
     1141 aggtaacgtt gacgcctggg ctatctggga tccctactac tccgctgcgt tattacaggg
     1201 cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa actggatcgt tttatctggc
     1261 agctcgcccc tatgcagaaa aaaacggcgc ttttattcag ggcgtactgg caacctttag
     1321 tgaggccgat gcgttaaccc gcagccagcg cgagcaaagc atcgctttac tggcaaaaac
     1381 gatgggctta ccggcaccgg tgattgcctc gtatctggat catcgtcctc ccaccaccat
     1441 caaaccggtg agcgctgaag ttgccgcctt acagcagcaa acggcagatc tgttttatga
     1501 aaaccgtctg gtgtcgaaaa aagttgatat tcgccagcgc atctggcagc ccactcacct
     1561 ggaaggaaaa caattatgag tctgaatatg ttctggtttt taccgaccca tggtgacggg
     1621 cattatcttg gaacggaaga aggttcacgc ccggttgatc acggctatct gcaacaaatt
     1681 gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga tcccaacggg gcgatcgtgt
     1741 gaagatgcgt ggctggtggc ggcgtcgatg atcccggtga cgcagcggct gaagtttctt
     1801 gtcgccctgc gtcccagcgt aacctcacct accgttgccg ctcgccaggc cgccacgctt
     1861 gaccgtctct caaatggtcg tgcgttgttt aatctggtca caggcagcga tccacaagag
     1921 ttggcaggcg acggcgtgtt ccttgatcat agcgagcgct acgaagcctc ggcggaattt
     1981 acccaggtct ggcggggcgc ttattgctgg gggaaagcgt cgatttcaac ggtaaacata
     2041 ttcatgtgcg gggagcaaaa ctgctcttcc cgccgattca acagccgtat cctccgcttt
     2101 actttggcgg atcgtcagat gtcgcccagg agctggcggc agaacaagtt gatctctacc
     2161 tcacctgggg cgaaccgcca gagctggtaa aagagaagat cgaacaagtg cgggcgaaag
     2221 ctgccgcgca tggacgcaaa attcgtttcg gtattcgtct gcatgtgatt gttcgtgaaa
     2281 ctaacgacga agcgtggcag gccgccgagc ggttaatctc gcatcttgat gatgaaacta
     2341 tcgccaaagc acaggccgca ttcgcccgga cggattccgt aggacaacag cgaatggcgg
     2401 cgctacataa cggcaagcgc gacaatctgg agatcagccc caatttatgg gcgggcgttg
     2461 gcttagtgcg cggcggtgcc ggaacggcgc tggtgggcga tggtcctacg gtcgccgcgc
     2521 gaatcaacga atacgccgcg cttggcatcg acagctttgt gctttcgggc tacccgcatc
     2581 tggaagaagc gtatcgggtt ggcgagttgc tgttcccaca tctggatgtc gccattccgg
     2641 aaattcccca gccgcagccg ctgaatccgc aaggcgaagc ggtggcgaat gattttatcc
     2701 cccgtaaagt cgcgcaaagc taaggaacac gatgatggca acgccagtga agaagtggtt
     2761 attgcgcgtt gccccctggt ttttaccggt gggcatcgtg gcggtgtggc aactggcctc
     2821 ctcggttggc tggctgtcga cgcgtatttt gccttcaccg gaaggagtgg tgacggcgtt
     2881 ctggacgctc tccgccagcg gcgaattgtg gcagcatctg gcgatcagtt cctggcgggc
     2941 gctgattggc ttttcaattg gcggatcgct gggcttgatt ctgggactga ttagcgggct
     3001 gtcacgctgg ggagaacggc tgctggatac ctcaattcag atgttgcgca acgtaccgca
     3061 tctggcgctg atcccgctgg tgattttgtg gtttggcatt gatgaatccg caaaaatctt
     3121 tctggtggcg ctcggcacgc tattccccat ttatatcaac acctggcatg gcatccgtaa
     3181 tatcgatcgc ggtctggtgg aaatggcgcg tagctatgga ttatccggca taccgctgtt
     3241 tatccatgtg atcctgcctg gtgccctgcc ctcaattatg gtcggcgtgc gttttgcgtt
     3301 gggcctgatg tggctgacgc tgattgttgc cgaaaccatt tctgccaatt caggcattgg
     3361 ttatctggcg atgaatgcgc gggagttttt gcaaacggac gtggtggtag tcgctattat
     3421 tctttacgcc ctgctcggca aacttgctga cgtcagcgcg cagctgttag agcgcctctg
     3481 gttgcgctgg aacccggctt atcatttgaa ggaggccacg gtatgaatac tgctcgtctg
     3541 aaccagggca caccattgtt gctcaatgca gtaagcaaac attacgcgga aaatatcgtc
     3601 ctgaaccaac tggatttaca tattccggca ggtcagtttg tggcggtggt gggccgcagc
     3661 ggtggtggca aaagtaccct gctgcgcctg ctggcaggtc tggaaacgcc aaccgcaggc
     3721 gatgtgttag cgggcaccac accgctggct gagattcagg aagacacgcg aatgatgttt
     3781 caggatgcgc gtctgctgcc gtggaaatcg gtgattgata acgttgggtt aggccttaaa
     3841 ggccagtggc gcgatgccgc gcgtcgggcg ttggctgcag taggactgga gaatcgcgcc
     3901 ggggaatggc ctgccgcact ttctggcggg cagaaacagc gtgtggcgct ggcaagagcg
     3961 ttgattcatc gaccgggttt attgttgctt gatgaaccgc tcggggcact ggacgcctta
     4021 acgcgactcg agatgcagga tttgattgtg tcactttggc agcagcacgg ctttaccgta
     4081 ctgttggtga cgcatgatgt gagcgaagcg gtagcaatgg ctgaccgggt gttgttaatt
     4141 gaagagggaa aaattggtct ggatttgaca gtggatatcc cccgtccgcg tcgattaggg
     4201 tcggtgcgat tagcggagct ggaagcggaa gtattgcaac gggtaatgcg gcgtgggcat
     4261 tctgaacaac tgattcggcg tcatggttaa tatgccggat gtggcgcaca aaaccagcat
     4321 tttcaacgga cttgtaggcc tgataagaca cgtcaagcat cgcatcaggc agttgggcac
     4381 ggctgccgga tgcggcgtga acgccttatc cagcctacaa aaccagcaat ttcaacggag
     4441 ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca ttttggctca aaagtatcaa
     4501 gccagtgctt tggtgatctt ctcgtacaga tcgccagaga gattttccag ccctttcaac
     4561 tgttccagcg ccgcgcgcat tttctcctga cgtttggcat cataacgttt caggcgaatc
     4621 agcggttcaa tcagacgtga agctacctgc gggttacggc tgttgaggtc ggtaagcatt
     4681 tccaccagga actggtaacc gctgccatct tcggcatgga acgctgccgg gttgctgccc
     4741 gcaaacgcac caatcaacga acgaatacgg ttcgggttgc tcatggtaaa tgagcgatgc
     4801 tgcaacagac cacgcaccgt ctccagcaca ttcgccgccg ggctggtggc ttgcaggata
     4861 aaccatttat ccatcaccag accgtcctga tgccacttat cgtcgtactc ctgcatcagc
     4921 gcgtcacggc aaggcagctg tgcagcaacc gccgcagaaa gcgctgccag cgcatcggtc
     4981 atattgtttg cttcatggta ttgcttgctc accagcacgt cagccagatg cgtttcacca
     5041 aaagcaagga agcgcaggca ggcatttcgc agagtgcgtt ttgcaatatc ttcatgctca
     5101 acacggtact cgctctggta attcgcgttg taaatagcca gcagctcatc cgccagttca
     5161 gtcgccagag tacgagtgag tgcttcgcgt acttcggcaa tagcaatcgg gtcgatgata
     5221 tcgaacagtt cagccatttc attgacagaa ggcagcgtca ggatttctgc cgccagcgcc
     5281 ggatcaatct tctcatcgag cagcaccgcg cggaaagcgt cagccacatg taccggcaga
     5341 gacagcggct gcccttgctg atgacgggcg acgttcagct tgatgtaggt tgccagcaaa
     5401 ctttgcgccg catcccaacg ggagaaatca ttacgcgcat gacgcatcag gaaggtcagt
     5461 tgctgatcac tccacttata ttccagtttc actggcgcag agaattcgca cagcagcgca
     5521 ggcaccggct ggaagtagac attatcaaaa acaaaggtct gttccgcctg ggtgacgttc
     5581 agcacggaat tcaccggatg accgcctttc tgcaacggga tcactttgcc ttcgttgtca
     5641 tacaattcga tggcaaacgg aatatgcagc ggctgttttt ctgcctgatc cggcgtggct
     5701 ggcgtgcgct ggctgatggt cagggtgtac tgctcagttt ccggattgta gtcgtctttg
     5761 acggtcacaa tcggcgtacc ggactggctg taccaacggc ggaaatggga gagatcaaca
     5821 ttcgacgcat cttccatcgc ctgcacaaag tcgtcacagg tcgctgcgct accatcatga
     5881 cgctcgaaat agagttgcat ccctttctgg aagttttctt cgcccagcag ggtgtggatc
     5941 atacggatca cttccgcgcc cttctcgtaa acggtcaggg tgtagaagtt gttcatctca
     6001 atgaccatgt ccgggcggat tgggtgcgcc atcgggctgg cgtcttccgc aaactgcaat
     6061 ccgcgcatgg tgcgcacatt attgatgcgg ttaactgcgc gggaaccaag gtcagagctg
     6121 aattcctgat cgcggaagac ggttaaacct tctttcaggc tgagctggaa ccagtcgcgg
     6181 caggtcactc ggttaccggt ccagttatgg aaatattcat ggccgataac gcgttcaata
     6241 tcgaggtaat ctttgtcggt ggcggtgtcg gtgcgggcca gcacatattt ggagttaaag
     6301 atattcagac ccttattctc cattgcgccc atattgaaga aatccaccgc gacgatcata
     6361 tagatgtcga ggtcatactc caggccgaag cgttcttcat cccatttcat tgagtttttc
     6421 agcgaggtca tcgcccacgg cgcgcgatca aggttgccgc gatcgacgta cagctccagc
     6481 gccacttcgc gaccagaacg cgtggtaaag gtgtcgcgca gtacatcaaa gtcgcctgcc
     6541 accagcgcaa acaggtagca cggtttcggg aacgggtcct gccactgtac ccaatggcgt
     6601 ccgttttcca gttcaccttg cgcaacgcgg ttaccgttgg aaagcaggaa gggatatttg
     6661 gttttatcgg caataatttt ggtggtaaaa cgcgccagca cgtccgggcg gtcgagataa
     6721 tacgtaatat ggcggaaacc ttcggcttca cactgggtgc aaagcgcatc gcctgactga
     6781 taaagccctt ccagcgcggt attcgccgcc gggcttattt cattaacgat cttaagcgta
     6841 aaatgctccg gcaaattact gatgaccagt gcgccctctt cttctttcca ggcggtccac
     6901 ggctcatcat taatatgaac agaaaccagt ttgaggtctt cgccgttgag acgaagtgga
     6961 gcatctgatg caccatgacg gacagcctgg ctgaccgcgg taacgaccgt cttttgcgcg
     7021 tcgaggtcaa aggtcaagtc aatatcagta atctggtaat ccggcgcacg ataatcgtga
     7081 cggtatttgg cttgtggctg ttgagtcata aataaccttt agcatctttt atagagtctg
     7141 gtgttcagtc tattcctgtt gcgtaaatcg cgctatgcag aatcttcatc ttttcaggta
     7201 caaacgcctt tattgctaca tttttataac atacaccgcg taatgccatc gaccagaaag
     7261 gtggcatatg gtgtgatcgg ggttcaataa attgcgaaac aaggtatact ccagcagttc
     7321 ctgaagatgt ttattgtact aaacgctcct gtacgaggac gctactgcgc acctatgaca
     7381 caattcgctt ctcctgttct gcactcgttg ctggatacag atgcttataa gttgcatatg
     7441 cagcaagccg tgtttcatca ctattacgat gtgcatgtcg cggcggagtt tcgttgccga
     7501 ggtgacgatc tgctgggtat ttatgccgat gctattcgtg aacagattca ggcgatgcag
     7561 cacctgcgcc tgcaggatga tgaatatcag tggctctctg ccctgccttt ctttaaggcc
     7621 gactatctta actggttacg cgagttccgc tttaacccgg aacaagtcac cgtgtccaac
     7681 gataatggca agctggatat tcgcttaagc ggtccgtggc gtgaagtcat cctctgggaa
     7741 gttcctttgc tggcggttat cagtgaaatg gtacatcgct atcgctcacc gcaggccgac
     7801 gttgcgcaag ccctcgacac gctggaaagc aaattagtcg acttctcggc gttaaccgcc
     7861 ggtcttgata tgtcgcgctt ccatctgatg gattttggca cccgtcgccg tttttctcgc
     7921 gaagtacaag aaaccatcgt taagcgtctg caacaggaat cctggttcgt gggcaccagc
     7981 aactacgatc tggcgcgtcg gctttccctc acgccgatgg gaacacaggc acacgaatgg
     8041 ttccaggcac atcagcaaat cagcccggat ctagccaaca gccagcgagc tgcacttgct
     8101 gcctggctgg aagagtatcc cgaccaactt ggcattgcat taaccgactg catcactatg
     8161 gatgctttcc tgcgtgattt cggtgtcgag ttcgctagtc ggtatcaggg cctgcgtcat
     8221 gactctggcg acccggttga atggggtgaa aaagccattg cacattatga aaagctggga
     8281 attgatccac agagtaaaac gctggttttc tctgacaatc tggatttacg caaagcggtt
     8341 gagctatacc gccacttctc ttcccgcgtg caattaagtt ttggtattgg gactcgcctg
     8401 acctgcgata tcccccaggt aaaacccctg aatattgtga ttaagttggt agagtgtaac
     8461 ggtaaaccgg tggcgaaact ttctgacagc cctggcaaaa ctatctgcca tgataaagcg
     8521 tttgttcggg cgctgcgcaa agcgttcgac cttccgcata ttaaaaaagc cagttaatat
     8581 catcagggag ctaatcggct cccttttttt acctttaatt ccgaaatctt tcgctgcatt
     8641 tgcgaattct gcttgtctga ttgcagatgc caggtaacat aggtatcccc cccatttacg
     8701 ggtggacaag tgtttatttt ttccgactat taacagagag aatattatga gcgttgtgcc
     8761 tgtagccgac gtactccagg gccgcgtagc cgttgacagc gaagtcaccg tacgcggatg
     8821 ggtacgtacc cgccgagatt caaaagctgg catctccttc ctcgccgttt atgacggttc
     8881 ctgctttgat cctgtacagg ctgtcatcaa taattctctg cccaattaca atgaagacgt
     8941 cctgcgtctg accaccggct gctcggtcat tgtgacgggt aaagtcgtgg catcgccggg
     9001 ccaggggcaa caatttgaaa ttcaggccag caaggttgaa gttgctggtt gggttgaaga
     9061 tccagacact tacccgatgg cggcaaaacg ccacagcatt gagtatctgc gtgaagtcgc
     9121 tcacctgcgt ccgcgcacaa acctgattgg tgccgtcgcg cgcgttcgcc atacgctggc
     9181 gcaggcgctg catcgcttct ttaacgagca gggattcttc tgggtttcaa cgccactgat
     9241 taccgcatct gataccgaag gtgcaggcga aatgttccgc gtttctacgc tggatctgga
     9301 aaacctgccg cgtaacgatc agggcaaagt ggatttcgac aaagacttct ttggtaaaga
     9361 gtctttcctg accgtatctg gccagttgaa cggcgaaacc tacgcttgcg cattatccaa
     9421 aatttatacc ttcggcccga ctttccgtgc tgaaaactcc aacaccagcc gtcacctggc
     9481 ggaattctgg atgctggagc cggaagtggc gtttgctaac ctgaacgata tcgcgggtct
     9541 ggctgaagcc atgctgaaat atgtcttcaa agcggttctc gaagaacgcg cagacgacat
     9601 gaaattcttc gctgaacgcg tagataaaga tgccgtttca cgtctggaac gcttcattga
     9661 ggccgatttc gcgcaggtgg attacaccga cgcagtgacc attctcgaaa actgcggcag
     9721 gaagtttgaa aacccggttt actggggcgt cgatctctct tctgagcatg agcgttatct
     9781 ggcggaagaa cactttaaag caccggtagt ggttaaaaac tatccgaaag atattaaagc
     9841 gttctatatg cgcctcaacg aagacggtaa aaccgttgcg gctatggacg ttctggctcc
     9901 gggcatcggt gagatcattg gtggctccca gcgtgaagaa cgtctggacg ttctggacga
     9961 gcgtatgctg gaaatgggcc tgaataaaga agattactgg tggtatcgcg atctgcgtcg
    10021 ctacggtact gttccgcatt caggtttcgg tcttggtttt gaacgcctga ttgcttacgt
    10081 aactggcgtg caaaacgtgc gtgatgtgat tccgttccca cgtactccgc gcaacgccag
    10141 cttctgattt tcttcctcaa agagccagcg cctgctggct cttttttatc ctttctgtca
    10201 agttatctgt ttgttaagtc aagcaatcta tttgcaaccc cgccataacg atcatcctgt
    10261 tacggaatat tacattgcaa catttacgca caaaaaataa cccgcattct tattgcggat
    10321 tagttttttc ttagctgata gcacaatttt catactattt tttggcattc tggatgtctg
    10381 aaagaggatt ttgtgccagg tcgataaagt ttccatca
//
LOCUS       DAZPJP010000139        10125 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPJP010000139 DAZPJP010000000
VERSION     DAZPJP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.139"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..613
                     /locus_tag="ACHU9J_003130"
                     /pseudo
     CDS             1..613
                     /locus_tag="ACHU9J_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /gene="prfH"
                     /locus_tag="ACHU9J_003131"
     CDS             610..1224
                     /gene="prfH"
                     /locus_tag="ACHU9J_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1906605.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="ACHU9J_003132"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="ACHU9J_003132"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1906606.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="ACHU9J_003133"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="ACHU9J_003133"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1906607.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="ACHU9J_003134"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="ACHU9J_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1906608.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="ACHU9J_003135"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="ACHU9J_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1906609.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="ACHU9J_003136"
     CDS             complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="ACHU9J_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1906610.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6634..7737
                     /gene="proB"
                     /locus_tag="ACHU9J_003137"
     CDS             6634..7737
                     /gene="proB"
                     /locus_tag="ACHU9J_003137"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1906611.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7749..9002
                     /gene="proA"
                     /locus_tag="ACHU9J_003138"
     CDS             7749..9002
                     /gene="proA"
                     /locus_tag="ACHU9J_003138"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1906612.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9117..9192
                     /locus_tag="ACHU9J_003139"
     tRNA            9117..9192
                     /locus_tag="ACHU9J_003139"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Thr,seq:cgt)
     gene            9798..9956
                     /locus_tag="ACHU9J_003140"
     CDS             9798..9956
                     /locus_tag="ACHU9J_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906613.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2505 a         2455 c         2632 g         2533 t
ORIGIN      
        1 gtctatttta cagcggcata ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga
       61 cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgc gtattaatcg
      121 ccagctgatc tctttacgca taatgcaaca ggttaaggcc accggaagtc cggttctgga
      181 tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac tcggtgatta
      301 ctcctagctt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 tgggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat acacagcgac
      421 gcagctctca cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 tggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga gcgattaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gcggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgttttgt tgaacagacg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag agaacggatc agacagtgaa tttctacgtt attatcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac gtctgagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt caggacgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ctgcatgacc cgctaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttccccgcc ggagtgaccc
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagccg
     2281 aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cgggccgtga
     2341 accacgtttt cgtcagccag aaccgccagc gcagacgcca taccaatgcc gttatctgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg atttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgcgagct taaagtgcta aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggcat tgacccacgt gaaatcctcg acgtccaggc gcgtattgtg atgagcgatg
     3781 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccatccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgtcac tggctgcatg cggctacgtt gtacaacatt gctgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttgtcaaac cgtgcctatg
     4021 aagaagccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcatcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgttttaaag gctctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaaatttg ccaatttggt aaaacagttg catcacaaca ggagattgca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgagttct ggggctggtg gatggagctt gaagcgcagg
     5041 aatcccgttt tacctacagt taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagacact gaagtggttg aacgactgga gcacacccta cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgatga accggtgaag ttaacggcgt gagtgaaatg tgccggatgt atcacatccg
     5281 gcaatatcca ttaaaactga taggtcatgc caaccgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaacgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattgac cagatcttca tcaccgatac
     5461 cttcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgtgtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 aatcgccacc aaattccggg aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttctg ctgtgcagta tcactctccg ctttatttcc ggcaaactcc gcttcccacc
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcacc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctggcgttat cactcatata atgcatggct ttaactttgc
     6241 catagacatc cagtttatta ccgtctttat tatatatttc tgcagcctgt acagatgcag
     6301 atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc attcctgatt
     6361 ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa gtttttaacc
     6421 tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt aatttattaa
     6481 atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc aacaaaacgc
     6541 catgctttgc tcgcagatgg ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat
     6601 atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta
     6661 aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc ccatattgtt
     6721 gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg
     6781 tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc cggaactgcc agcgactatc
     6841 gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact gtgggaacag
     6901 ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa
     6961 gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga taacaatatc
     7021 gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt cggcgataac
     7081 gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc
     7141 gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa
     7201 gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc
     7261 ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg tgcgggtatc
     7321 gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt
     7381 tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt
     7441 ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg
     7501 gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt
     7561 ggtgaagtca tccgcatttg caacctcgaa ggccgcgata tcgcccacgg cgtcagtcgt
     7621 tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata
     7681 ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga
     7741 gctggccgat gctggaacaa atgggcattg ctgcgaagca agcctcgtat aaattagcgc
     7801 aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac
     7861 aaagcgaaat catcctcaac gctaacgccc aggatgttgc tgacgcgcga gccaatggcc
     7921 ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa ggcattgccg
     7981 acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc gatggcggcg
     8041 tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggggtt attggcgtga
     8101 tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta
     8161 atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg
     8221 tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag gcgattgata
     8281 atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc gacatgctga
     8341 tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca atcccggtga
     8401 tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag atcgctgaag
     8461 cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat acggttgaaa
     8521 cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc aaacaaatgg
     8581 cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg caggcaggcc
     8641 ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg tcattagatt
     8701 tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt gaacacggca
     8761 cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa cgcccagcgt tttgttaacg
     8821 aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac ggcggccagt
     8881 ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg
     8941 ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc attcgtgcgt
     9001 aaataaaacc gggtgatgca aaagtagcca tttgattcac aaggccattg acgcatcgcc
     9061 cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct ttcagggccg
     9121 atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     9181 ttatcggcac cattaagaaa attaataact tagcttaact tctctaagaa attgtatctt
     9241 ctgttatcac ttacaaacgc ctatacgttg ttatgggctt tggtagtgta acacgtatgg
     9301 gcgagtagtt cccaggctca acctcgtaac aagttccttt gaagctggaa gacgtgccac
     9361 cgtctctaaa ccttctacca aaactcttag caagatgttc cacacctggc gcggtgtcca
     9421 atgatgctac acgtcgtgag acgttgcaga ggataaacag gctaaatgat gggaagagtt
     9481 ttaagtgcaa tagtggatta aagcccacct cttcaaaacc taacttgatc gggaaagaat
     9541 caagctctag cacggtttcg tccaccggag tacaacgggt gcctgataag agaagaactg
     9601 ctaagacttc atagcttttg tgaaaaagcc ctttttagtg gatacgccta cggcgcttat
     9661 tttttggtat aaccatcaaa ggcgctttat ggctcctatt gtcttgaaaa tgtatcttta
     9721 cttgtattgt gttttatgcg tagtctccct gtgtagtatg taacttgtta attttcatat
     9781 gaatacagag gcacacgatg aaaaacattg cagcaatcaa acgtaataac cgcaagattc
     9841 acgctcgtaa gttcctgtct acgccagaag gaaaagcctg gctagagcgt aaacagagag
     9901 agaacgaaga aagaaaactc cttagtgagt tgaaatggct tagagaggat ttctgaaaat
     9961 ctccagaacg caacaggacg cattctaacg ctgttttcat tactcggtat agggataggt
    10021 gatgtgtgtt taatgcgtct gtgcgcttgt tttgtgcctt attttgcatt ttcataatgc
    10081 ggaagagaac acggaaaacc ttaaaattaa ttattggggt attta
//
LOCUS       DAZPJP010000140        10015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPJP010000140 DAZPJP010000000
VERSION     DAZPJP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.140"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(267..342)
                     /locus_tag="ACHU9J_003141"
     tRNA            complement(267..342)
                     /locus_tag="ACHU9J_003141"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Asn,seq:gtt)
     gene            complement(443..1240)
                     /gene="mtfA"
                     /locus_tag="ACHU9J_003142"
     CDS             complement(443..1240)
                     /gene="mtfA"
                     /locus_tag="ACHU9J_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1906614.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1334..1423
                     /locus_tag="ACHU9J_003143"
     tRNA            1334..1423
                     /locus_tag="ACHU9J_003143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Ser,seq:cga)
     gene            complement(1993..2523)
                     /locus_tag="ACHU9J_003144"
                     /pseudo
     CDS             complement(1993..2523)
                     /locus_tag="ACHU9J_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(2866..3516)
                     /gene="zinT"
                     /locus_tag="ACHU9J_003145"
     CDS             complement(2866..3516)
                     /gene="zinT"
                     /locus_tag="ACHU9J_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1906615.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3773..4408)
                     /gene="msrQ"
                     /locus_tag="ACHU9J_003146"
     CDS             complement(3773..4408)
                     /gene="msrQ"
                     /locus_tag="ACHU9J_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1906616.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(4409..5413)
                     /gene="msrP"
                     /locus_tag="ACHU9J_003147"
     CDS             complement(4409..5413)
                     /gene="msrP"
                     /locus_tag="ACHU9J_003147"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1906617.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(5522..5935)
                     /gene="hiuH"
                     /locus_tag="ACHU9J_003148"
     CDS             complement(5522..5935)
                     /gene="hiuH"
                     /locus_tag="ACHU9J_003148"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1906618.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            6068..6739
                     /gene="hprR"
                     /locus_tag="ACHU9J_003149"
     CDS             6068..6739
                     /gene="hprR"
                     /locus_tag="ACHU9J_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1906619.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            6739..8097
                     /gene="hprS"
                     /locus_tag="ACHU9J_003150"
     CDS             6739..8097
                     /gene="hprS"
                     /locus_tag="ACHU9J_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1906620.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(8205..9056)
                     /gene="hchA"
                     /locus_tag="ACHU9J_003151"
     CDS             complement(8205..9056)
                     /gene="hchA"
                     /locus_tag="ACHU9J_003151"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1906621.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            9203..9613
                     /locus_tag="ACHU9J_003152"
     CDS             9203..9613
                     /locus_tag="ACHU9J_003152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906622.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(9647..>10015)
                     /locus_tag="ACHU9J_003153"
     CDS             complement(9647..>10015)
                     /locus_tag="ACHU9J_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1906623.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT         2825 a         2065 c         2297 g         2828 t
ORIGIN      
        1 agcgaaaaaa tgtaaatact gatagcaata cgattgaaaa ttaataactt ttcgttaggc
       61 agttttgggt gtgagttgca agaggggaga ctactgaata actcaagttt tataatcgag
      121 gggaaaatgg tgatggcgtt catagcaaaa cgccctcaac cataaaggtc gagggcgctt
      181 aaggtgttaa aaacccgcta tccgttaaaa aacaatgttc aactaaggtc agtgacattg
      241 cgctaaaaaa gcgaattttt agaatttggc tcctctgact ggactcgaac cagtgacata
      301 cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgtg aacggggcac
      361 atattactta gcggtacctt gtctgtcaac actaaatttc ataagtcatt tcaattggtt
      421 aattaatctg caaagttgtt aattaatgaa cattcgtcgc cgaaaacgag tctgtatcat
      481 tagcgtgatg cagtctctgc aaaggatctt gttgataaaa ttggcagaaa cgttgccaca
      541 atgaagggaa acgaggagca aaaagttctg gggcgctaaa gaaatattca gaaagtacgg
      601 caaaacattc agcaggatcg ctggctgcat aagcatcaat gctcgccgca ttctcgccaa
      661 ccaattcgat ttcttcctga atgttgttca ttgcagcatg aagatcgtgt tcccagccag
      721 caacctcacg caacggaata aagggaactc cgctggcgcg atcgccgtta cgggtgtcca
      781 gcttatgagc gacttcatga ataatcaggt taaaaccaga tgcatcaaaa gaatcttgta
      841 tatccaacca gttcaaaacg ataggccctt gctgccagct ctgacctgac tgaacaatac
      901 gttggttatg caccagaccg atatcgtctt cccattcatc atcgaccaca aatggcgcag
      961 gataaattaa gacttcatga aaaccatcca gccattccag tcctaactcc agaacgggta
     1021 ggcaaaatag aagtgctatc cggcagcttc ttaatgaatc cagttcaaag ccctgtaaag
     1081 gaacaagccg cttttgctgt aaaaaacgtt cggcaagagt gactaatttg ctttgttcct
     1141 gttccgtcag acccgttaaa agggggatcg atagtgcttc ctgccaggga agggcagttt
     1201 gatgtgctga ttcttgtact ttccagggcc acttaatcat cgttttgctc gcaaactcgt
     1261 cacttgaaca aaattgcacg gacagggact gttaaaatgc caaatttcct ggcatcatgg
     1321 caaccatctg aacggagaga tgccggagcg gctgaacgga ccggtctcga aaaccggagt
     1381 aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactttatc aatgacttat
     1441 ctcctgactt cccgccttgc ttttcctaaa cagaacaatt gtagaatatt cttgaagggt
     1501 tagatcgtca ctgttttctg ttcgatactg tgacattcag cacttgattc gctatggatc
     1561 tgacaggaag ctttcgagcg aaaatgtgca gttattcagt cgttttctta tcgctcacca
     1621 ttattctttt agacattcat cctatgaaac tgccgcaaag ttggtggtgg gaactgaagt
     1681 tgcgtagaga aggggtcaat aattgagtca aaaaaggaac gcggccgtca gattaaagcc
     1741 gcgggacaaa gtccataatt tccaagagtt gttgaatttc ccagcttttt tgaaagcctt
     1801 tgtatcatac taacaagtaa aaaaggttta aataccaaaa ttaacctgta tttttgataa
     1861 gtcttaccat tgtacgattt acacagtttc tgctcctcct tgttgatagt agaaaggatg
     1921 cattgtgcac ttgcgtaaac atttatgacg cgttgatgta catggttcac atttccttgt
     1981 caggggatcg acttatttgc ggcgatgcat cattcgtaac agagtattat ctttctgaat
     2041 gtagtgatga aagagtgctg cgccagcgtg agctgcgatt aaaaagtaac ctatatttgc
     2101 ctaggtttcg tgaatatttt ttatcagtgc tttaatttcg ctgtttgggg taacaaaggg
     2161 agacacattg aaaccaagga aactccacga ttttccactg taagccatca aagcaatccc
     2221 cagcagagga agtgcaagga aggttatata aagcatgatg tgcattagcg aagccgcttt
     2281 catttgccag gcaggtggcg gtggtacaat agaaggatca ctataacggt gttttataat
     2341 caggcgtgaa aacattaaca cccaaacgaa tatacccgca ttgtaatgtg tttctcgcat
     2401 cagcagataa gtactactac ctttaggaaa ccagccacgg agttccatcg cggcataggt
     2461 tattgcaacg aaaagcagcg ttatccaatg taaataaatt tgagtttttg agaatcggtt
     2521 catttttgta gctcctgtaa tttttacagt caagctatcc cacgaacctt aaaaattcct
     2581 tatacagatg tggttttaag aatttgttat ttaaaaaatt gatagtgcac attgtaacca
     2641 gccaatataa gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg
     2701 aatccgttat tggttaactc aatgcgggtt agcttattaa tatacagaca cgtcatagtg
     2761 atgagcgtgt ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt
     2821 atgatgactt actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat
     2881 ttcctcgacc acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat
     2941 ttcatttaat aaaaattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt
     3001 tcgtggtgca attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc
     3061 tttacattcg aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta
     3121 tccatcgtaa tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc
     3181 gtcctcaatg ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat
     3241 ttcagcaaat gttttaattt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt
     3301 gccactttgc agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg
     3361 gttttgtaca ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa
     3421 gggtttgccg tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc
     3481 taaagcaaca gccagtttgt gaagacgaat tgccaaaaca gtttcctcca gagtcattgt
     3541 atatgatgtg taatgttata ttgtaacata tggcaaaatg caacaactct taggagatga
     3601 gagcatagtt ttctcaggtg tacttatcta tcttattgaa ataaaatgaa ttatgttgat
     3661 aatttgaggt tgatgacaaa cgaaacataa cctgatgtga tctgcgcagg ccggacaggg
     3721 cgctcgcgcc acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag
     3781 ataatttgtt gtgaacctgc ttgcgtaacc gcttaaaaag ggaacgcaac ttcttatacc
     3841 gtaaggctaa aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct
     3901 tcactgacca cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca
     3961 actgttgcca gtgcttgccg agttttcgct gcatcgcctg ggttgaagta aacgctaaag
     4021 caagcaaaat aacccagctg ataattccta acgttaaata aggccgggta attaactcct
     4081 tgcctaataa cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca
     4141 gtgtcgccca ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct
     4201 gttttgcgta gcgtgccaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag
     4261 tgcgaccggt aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga
     4321 tcgcccagac cagccagaga aacggcaaca atccggcaag atgcaggcta actttcagcc
     4381 atgtcacctg ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat
     4441 acagcgatgc cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgaacat
     4501 cgaggatgcc gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa
     4561 catgcggatt aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg
     4621 ttggcggacg ctcgcgggtc agcttaatac tgactatcga tttaatccct ttaaagccat
     4681 atttccacgg cacaatcagt cgtaccggcg cgccattttg tggcggtaac gccttgccat
     4741 aaacacccac ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga
     4801 cataaggata tttcagcccg ccgccgataa agcggtcctg ctggcctggc atttgttccg
     4861 gtgcataaat tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca
     4921 gcaatttgtg cagcggaaaa ccaatccacg gcaccaccat cgaccatgcc tccacgcagc
     4981 gcatacgata aatacgctct tccagcggga agcgacgggt taaatcatcg tgatcgaggg
     5041 tcaatggttt tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac
     5101 cagcattagc ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg
     5161 agactttatc tactggcgtc agtggcagat tattttgcca ggcggtaggc ttgctgaact
     5221 ccagcggttt tccggcgggg gcgggcgggc gatcgttccc tttaaaccag ctaagcagat
     5281 cggcatgcgc agcgtgaggc aaagaaagtg cagctgcgct gatgcccagt gcttttaaca
     5341 cctgtcgacg cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt
     5401 gattcttttt cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt
     5461 taatctgtca gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc
     5521 attaactgcc acgataggtt gaatacccat attggctaag taataaaggc acatgataat
     5581 gctcgttcac tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat
     5641 tttgtttctt gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag
     5701 tttgctcggg ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt
     5761 gtaaccagcc gttgtccgct ttcttttcaa gagtgactgt cacgtcggca gcgggttttc
     5821 cggtttgctg gttcaaaatg tgcacgctaa gaatgttttg ttgtgcggca taaaccaaag
     5881 aaggtaatga aaatgctgcc gttactacgg agagtactaa ataacgcttt aacatgttta
     5941 tatccttgtc atgtgaatga gttttgagga tagcgaaaaa gcggccaggg taaccttaca
     6001 gcagcattac aattttgtaa tgaactaggc tgtttctata acatatgatt tatggcatat
     6061 tattttcatg aagattttac ttattgaaga taatcaaagg acccaggagt gggtaacgca
     6121 ggggctttcc gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta
     6181 tcttgcgctg aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga
     6241 tggctggcag atcttacaaa cgttaagaac agcaaagcaa acccctgtta tttgccttac
     6301 tgcaagggat tctgtcgatg acagagtcag agggctggac agtggagcaa atgattatct
     6361 ggtaaaacct ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taaggcaaca
     6421 tcacgctttg aattcaacat tagaaatcag tggcttaaga atggactctg taagtcaaag
     6481 tgtgagcagg aacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct
     6541 actggcctcc agagctggcg aaattatacc cagaacggtt attgcgagtg aaatttgggg
     6601 aatcaacttt gatagtgata ccaatacggt ggatgtcgcc attcgcaggc tccgcgcaaa
     6661 agttgatgat ccttttcctg aaaagctaat cgccacaatc cgggggatgg gctattcatt
     6721 cgtagcggta aaaaaataat gaaaagatta tctataaccg tccgtttaac cttgcttttt
     6781 atattgctgc tgtctgttgc tggcgctgga attgtctgga ctctctataa tggcctggca
     6841 agtgagttga aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag
     6901 ttgttaattg atggggtaaa tccagatacg ttacctgtat actttaaccg gatgatggat
     6961 gttagtcagg atatcttgat cattcatggt gatggcatca ataaaattgt taaccggaca
     7021 aatgtcagtg atgacatgtt aaataacata cctgctagtg agacaatcag cgcagctggc
     7081 atttacagaa gcattattaa tgatactgag attgatgctt tacgaattaa tattgatgaa
     7141 gtttcgccat cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt
     7201 gaacagtata aaattaatag cattataatt tgcattgtcg ccattgtact ttgctcagta
     7261 ttaagtccgc tgttaatcag aacgggatta cgagagatca aaaagttgag tggtgtaacg
     7321 gaagcgctga attataacga tagccgggag cctgttgagg ttagcgcatt accgagagaa
     7381 ctaaaacctc ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa agattttgag
     7441 cgtctaagtc aatttgctga cgatctcgct catgaactta gaacgcccat taatgcatta
     7501 ctgggtcaga atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca
     7561 attgccggta acattgaaga gctggaaaat atttcgcggt taacagagaa catactgttt
     7621 cttgcccggg cagataaaaa caatgttttg gtgaaactgg actcgctttc tctcaataag
     7681 gaagtcgaaa atttgttgga ttatcttgaa tacctttcag acgagaaaga gatttgcttt
     7741 aaggtcgagt gcaatcagca aatctttgcg gataaaattt tactgcaacg aatgttatcg
     7801 aatcttattg ttaatgccat tagatattca ccagaaaaat cgcgtattca tataaccagt
     7861 tttcttgata ccaacggcta tcttaatatt gatgtcgcca gtcctggaac gaaaattcat
     7921 gagcctgaaa aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt
     7981 cagggacttg gtctttcttt agtcaaagcg attgccgaat tacatggggg aagtgctacg
     8041 tatcactatc tcaataagca taatgtgttc cggattatgt taccgcaaag aaattaacat
     8101 agggcttcag tacgccataa atcaaccatg aaaaaacgca gccacgtcgt gagtattaac
     8161 gtggctgcga ttgattatgc gtttacattc aaacgtaaca gggattaatc cgcataagct
     8221 gccagcattt cctgcgccgc cagtttaccc aacgcattcg ctgcgaaagg actgtcgccg
     8281 gtgagaactt tacggtcctt atgtactcgc ccggtgatgt cgtcattaat aatgttcatg
     8341 cccattttct tcagctcttc gccgaagtac caggtgagat gacccggcat atagcctatc
     8401 tctggcgttt gtttgtctgc ggcgtctggg aatgcgcaaa tggaataacc attcagtggg
     8461 ttatcgccgt ggcgaagcgc cagaaaagcc gccgggccgt ggcaaaggga gataacaaaa
     8521 cggtcatttt tgattgccca ctgcaaagca gcagctacgt cctggctttc tggtaaacca
     8581 ataagtgcgc catgaccacc aggaacaaag attgctgcat attcgctatc agcgttgagg
     8641 ctggcgacaa catccgcgag tttcttcgga ttgcggaaca acgatttatg ctgctcaaag
     8701 aatggcatta ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga
     8761 ccggaaatgg tcgccacttc gaattcgaaa ccagcagcat ggagatgata caacggcagc
     8821 aacgtttcaa tcggatggtt accggtcgag aacagttttc cgttatcggt tggcaaataa
     8881 cgttcgtccg cggcgatcac cagaatttta tgcttaccgc gatacggttt tggatagtcc
     8941 acgccatcaa gatcagagac gggactggta tattggctaa gcgaatattc tgaagggaag
     9001 aatgcattat cttcagcaat atcgacctgc ggatttttac ttgtttgaac agtcatagtg
     9061 tattccttat tgttgcttag ttagggtagt cactatattt gcgactgagc tggtcgctat
     9121 ttttaaccag tctggaaaat tgagtcaata tatcgaaagc ggtagtcggc gggtgtaagt
     9181 atgtaatgat gatgctgcac tggtgttgaa tattattcac gccaaatttt ccttattgag
     9241 caacgtcgtc gcaagtggcc tcaatgattt tagaccatta tcaaaggtac ggggcggcgg
     9301 ggagagattt agtgctgtga aatattgtgg tactgatgaa tatttatatc gcggattggt
     9361 agagaaaaga ttatttcatg cactattttt gcaaatattt ctatatggga agcgtgtttg
     9421 ttacggatta attcataaag aaatgcattt gggtgttggt ttgaagagtc gctggttgat
     9481 gttattgtgc caggaggggc agggtaaggt gctgtatcga tatgcattgg caattgcgtg
     9541 tgccgcagat ggagatgaaa tgccaataaa aaagcctgaa ttcgatgagg gacgaactca
     9601 ggctagtgtt tagttttaca gtaacacgcc gcagcacgtt ggctgattag aactggtaga
     9661 ctaagcccac accaacaacg tcatctgttg cgatgccatt gttgtcatag aaatcgtcat
     9721 ctttatccaa caagtttatt ttatagtcaa catatgttga gaagttctta ttgaaatagt
     9781 aggtcgctcc aatttcaaca tagttgacca gatcctgatc aacatactct tgatgaccat
     9841 tgttgttata gtcgttaccg ccgaggtctt tgccttttga ataaacatat gcaagagatg
     9901 ggcgcagacc aaaatcgaac tgatactgtg ctacagcttc aaagttctgg gttttatttg
     9961 cgatacccac attaccatat ggtgtcatat tgcgggtttc agcatacatc attgc
//
LOCUS       DAZPJP010000141         9990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPJP010000141 DAZPJP010000000
VERSION     DAZPJP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.141"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            2..895
                     /locus_tag="ACHU9J_003154"
     CDS             2..895
                     /locus_tag="ACHU9J_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1906624.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            1030..2250
                     /gene="mlc"
                     /locus_tag="ACHU9J_003155"
                     /gene_synonym="dgsA"
     CDS             1030..2250
                     /gene="mlc"
                     /locus_tag="ACHU9J_003155"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1906625.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2375..3070
                     /gene="bioD"
                     /locus_tag="ACHU9J_003156"
     CDS             2375..3070
                     /gene="bioD"
                     /locus_tag="ACHU9J_003156"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1906626.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHU9J_003157"
     CDS             complement(3023..4315)
                     /gene="clcB"
                     /locus_tag="ACHU9J_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1906627.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHU9J_003158"
     CDS             complement(4474..5088)
                     /gene="dmsD"
                     /locus_tag="ACHU9J_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1906628.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHU9J_003159"
     CDS             complement(5131..5985)
                     /gene="ynfH"
                     /locus_tag="ACHU9J_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1906629.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(5989..6603)
                     /locus_tag="ACHU9J_003160"
                     /pseudo
     CDS             complement(5989..6603)
                     /locus_tag="ACHU9J_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
     gene            complement(6614..9037)
                     /gene="ynfF"
                     /locus_tag="ACHU9J_003161"
     CDS             complement(6614..9037)
                     /gene="ynfF"
                     /locus_tag="ACHU9J_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR1906630.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9098..>9990)
                     /locus_tag="ACHU9J_003162"
     CDS             complement(9098..>9990)
                     /locus_tag="ACHU9J_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR1906631.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2449 a         2553 c         2593 g         2395 t
ORIGIN      
        1 tatgaatatt gaacttcgtc atctgcgtta ctttgttgct gttgcggaag agctgcattt
       61 cgggcgcgcc gctgcccgcc tgaatatttc gcaaccaccg ctaagtcagc agattcaggc
      121 actggagcaa caaattggtg cccgactgct ggcacgaact aatcgcagtg tattgctgac
      181 ggcagcagga aaacagtttc ttgcagatag tcggcaaatc ctgtctatgg tagatgacgc
      241 tgccgctcgc gctgaaagac tgcatcaggg tgaagcgggg gagttgcgca ttggttttac
      301 ttcgtccgct ccttttattc gggcggtgtc cgatacgtta tcgctgtttc gccgtgatta
      361 tcccgatgtc catttacaaa cccgcgaaat gaacactcgt gagcaaatcg ctccgctcat
      421 tgaaggaacg ctggatatgg gattgctgcg taacacagcg ttaccggaga cgcttgaaca
      481 cgcagtcatc gtccatgaac cgcttatggc gatgatcccg cacgatcatc ccctggcaaa
      541 taacccgaat gtaacgctgg ctgaactggc gaaagaaccc ttcgtctttt ttgatccgca
      601 cgtcgggaca gggctgtatg acgatattct cgggctgatg cgacgttacc atttgacgcc
      661 cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct
      721 gggtgtttca attttgcctg cttcatttaa acgtgttcag ctcaacgaaa tgcgctgggt
      781 gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga
      841 acaaagtcca gcttcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa
      901 atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac
      961 gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg
     1021 gagtatgcgg tggttgctga aaaccagctt gggcacattg atcaaataaa gcagaccaac
     1081 gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc
     1141 cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat gctcgaagca
     1201 cacctggtgc aagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg
     1261 ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt
     1321 ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg
     1381 ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca gttttttatc
     1441 cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt
     1501 gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg
     1561 ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca tgatatcagc
     1621 gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag
     1681 gtggttatcg atcacaacgt gggggcgggc gtcattaccg atggtcatct gctacacgca
     1741 ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg taaacgctgt
     1801 tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg
     1861 gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg
     1921 gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat cattaccggg
     1981 gtgggcgcgc atgtcgggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa
     2041 atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttccctgt catctcggac
     2101 agcatacgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag
     2161 ttttctaacc agggcacgat ggcaggcgct gcgctggtaa aagacgcgat gtataacggt
     2221 tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg
     2281 ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctag
     2341 tttatatttg tgaagcataa cggtggagtt agtgatgctg gagcgtttct ttattaccgg
     2401 tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc
     2461 ccagggaaaa acagttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga
     2521 agggctgcgt aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg aactgcctta
     2581 tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc
     2641 aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag tcgatcatgt
     2701 cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc cactctctga
     2761 atgggtggtg caggaacagt tgccggtgtt gatggttgtc ggtattcagg aaggttgcat
     2821 taaccatgca ctgctaacag ctcaggcgat tgccaacgac gggctgccgc tcattggctg
     2881 ggtggctaac cgaatcaacc caggactggc gcattatgcg gaagtcattg atgtgctggg
     2941 taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg
     3001 agaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt
     3061 cacggtgtaa cgtccgcgaa attaccgacg caatgacgca ggcaatcaat aaaccgggga
     3121 gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg
     3181 tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc
     3241 catcagggaa ccataatccc aggctacgac catacaacat gccaatggca agaccgataa
     3301 atagcgtcgg tgtaaagaca cctccgggtg cgccggaacc gctactcgcc agcacggcac
     3361 acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg gttaaaaagg
     3421 attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca
     3481 cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac
     3541 gatgacaggc gttcattaac gttaacaaca gtggtccaca cagacctgcc agcacacctg
     3601 tactgataat cagcgcatag tcactagcct gaaccgttac tgagagttgt acgctgtaga
     3661 gtaacgcgtc gctatgatta ataagattgc taaccagcaa cgccacgacg gcggaaataa
     3721 tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac
     3781 tgccagcaag cggggcgcga taggccgcag ccattcccgc cgcggcccca caggcgatcc
     3841 ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag
     3901 ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagcg
     3961 aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt
     4021 ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag cccatcagca
     4081 acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt
     4141 ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca
     4201 gcatcgcatg acgaaacccg gcaacggcaa aggccgcgag aataccgacg actgttgcga
     4261 taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca
     4321 gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca
     4381 gtgcgtgctt taagagataa aaaaacgtga cattgtaact tattattgcc gaatcgcgcg
     4441 taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt taaccgcgac
     4501 aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc
     4561 acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg
     4621 aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca
     4681 cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa
     4741 ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac
     4801 agattcgcga tccagccaga ccgatcccca cggcggagac ggcagtgccc acgggccgac
     4861 aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac
     4921 cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag
     4981 taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa
     5041 cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct
     5101 gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc ctactgtcat
     5161 gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga ggattagccc
     5221 cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa gccagcatcc
     5281 aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag
     5341 tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt gcagcacaat
     5401 caccccggca caagcgatca gcgcgagaac gctaatgatg gcaaatggtg tggtattaaa
     5461 tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca acaataccgt
     5521 caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacagtgt cgatttgata
     5581 cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag
     5641 tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac
     5701 ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt
     5761 cagcgagtta aacgcccgca gtggcgatcc caggtgcatg acagaggcca taaacccgat
     5821 gcccatcaac agccagagga aaaacatgcc acggacaata cgctgacggt ctgcgtcatt
     5881 tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa
     5941 tacggtaaag ataaccagtg gccactcatg ccatccattt cccattttaa cttcctccgg
     6001 attagccaga taaccagtgg tatcgctggt cgggcggctg ttggcgttag gtttgataac
     6061 gatattgggt tttgtgaaat gcgcgcgcgg cagtggcgcg acggcagcca gagtgccgtg
     6121 cttctggcgc agctcttcaa ttggaccgaa ctcaagtgca cgcagcgggc aggattcgac
     6181 acatatgggt tgtttcccct cggcgacgcg cgaataacaa ccatcgcact tcgtcatgtg
     6241 ccctttttca gcattgtact gtggcgcgcc gtacggacaa gccatgtggc agtagcggca
     6301 gccgatacaa acatcttcat caaccaccac aaagccatct tcacgcttat gcattgcgcc
     6361 gctggggcag acttttgtac atgcggggtc atcgcaatgg ttacaggaga tggagagata
     6421 ataagcgaac acgttctggt gccagacgcc attatcttcc tgccagtcgc cgccagcata
     6481 ttcataaata cggcggaaac tgacttccgg gccaagatct ttaaaatctt tgcacgccag
     6541 ttcgcaggtt ttacagccag tacagcggct ggaatcgata aaaaatccat attgtgtggt
     6601 cacgggctac tccttaaacc ttttcgatct ggacaagatt gctgtgcgac gggtttccct
     6661 ttgccagcgg tgaagggcgg tgagaggtca gaatattgat actgccgccg tgatcgaccc
     6721 ggtcaccaaa catatccgct ttaagccaag caccttgccc gatggcggta acgccaggca
     6781 gaatacgcgg agtcactttt gcggcaatca gcatttctcc attattgtta aatacccgca
     6841 cggtatcgcc atgacggata ccgcgtgcct gagcatcaat ggggttgatc cacacctctt
     6901 gtgggcaggc ctgctgtaac acatcaatat tgccgtagct ggagtgggta cgcgctttgt
     6961 aatggaagcc cgttaactgc agtggatagg ttttccgcag gggatcgtcc cagccatcaa
     7021 aatctggggt atacgcagga aggggatgaa taacttcatc ttttttcaat tcccaggtat
     7081 ccgcaatctt cgccagtcgt tcagaataaa tttcgatttt ccccgaaggt gttttcaacg
     7141 ggtttgcctg tggatcttca cggaatgcgc ggaaagcgac gtagtgttct tccgggcatt
     7201 ttttcttaaa gatcccggtc gttttcatct cctcgtagtc gggcatctca gggttacgtt
     7261 ccttcgtttt cgcatggaga tatttgatcc attcatgctg actgcgacct tcagtaaagg
     7321 tttgataaac gtctggtcct aagcgtttgg cgacttcact cagcatccag tagatgggtt
     7381 tgcgttcaaa ttttgctgag gttgcgggtt gggcgaggat cacatagccc atattccctg
     7441 cagattcatg agagataagg tcttcttgct ctgttggcat caggtcgggc aacaggatat
     7501 cgcaatactt agccgaggcc gtcatgaagt ggtcaatgcc aacaatcatc tcgcacttgc
     7561 tgtcatcctg aagcacctca tgggtgtgat tgatgtcgcc atgttgattg atcaatgtgt
     7621 tactggcgta gcaccataaa aacttgatgg ggacatccag tttttctttt ccacgaacac
     7681 catcacgggt cgcggtcatt tccgtaccat ggtcgatggc atcagtccat gtaaagacgg
     7741 aaatctgcgt tttaacagga ttctcgagca tcgggaacca ttctaccccc agatcccagc
     7801 taccttcgcg tacgcctgag ttgccgccgt ttatgccgac gttaccggtg agaacggaaa
     7861 gcatggcaat agcgcgggat gtttgttcgc cgttggagtg tcgttgtggc ccccaaccct
     7921 gacaaatata agcaggtttt gctgaaccga tctctcgtgc caactggata attttttctg
     7981 ccgggatgct ggtgattttt gctgcccatt ccggcgtttt agctatgccg tcaggccctt
     8041 cgcccagaat ataggcttta taatgcgcgt tacgtggtgc gttggcgggc agcgtttttt
     8101 catcgtaacc aacacaatat ttgtcgagaa atggctgatc gaccatgttt tcagtaatca
     8161 gtacccaggc aatcgcacag gccagtgcgc catcggtgcc agggcgaatg ggcagccatt
     8221 catcttcacg cccggcagct gtgtcgttat aacgtggatc gatgacgatc atgcgtgcgt
     8281 ttgaacgttc gcgggcttgc tcgacgtagt aagtgacacc accaccgctc atccgcgttt
     8341 ctgccgggtt atttccgaac ataacgacca gtttcgtatt ggcgatatca tccgggctgt
     8401 tgccatcatt ggcaccgaac atataactca ttgcggcact gatctgtgcg gtactgtagc
     8461 tgccatagcg actgagaaaa ccaccgcaag agttcatcag acggtacggg acgtttgagt
     8521 tggtgatgtt tccgccatct acacctgttc cgtacaggac atgtacagct tcattgccgt
     8581 aatcttttag gatccgccga agattatcac tgatggtatc cagggcttcg tcccaactta
     8641 tccgttcaaa tttaccttca ccgcgcttgc cgacgcgctt catgggatat ttcaacctat
     8701 caggatgatt catccgtcgg cggatagagc gcccgcgtaa acacgcgcga acctgatgat
     8761 taccgtagac gtcgtcacct gtcgtatcat actccaccca gtacacggtg tcatctttca
     8821 catgcaaacg taacagacag cggctcccgc agttaacggt gcaggaactc cagaccgctt
     8881 tctcttctac cggagcctct gccgcccgaa ccatttggga aaatggcaga gtgaaagcac
     8941 tgcttgccag cgcaaggctg ccaagtgcgg aggttttcat cagacttcta cggctgattt
     9001 cagccttcat gagcgcctct gtggtatgga ttttcatcat tactcactta ttgcttttca
     9061 aacaaaatgt catgccagaa tttatggttg tagtgggtta tattttttcg atctcgacca
     9121 gattagtgtg ctgcgggttt cccttcgcca gtggtgaagg gcgcagagtg gttagcgtat
     9181 tcacacagcc gccatggtcg attttatcgc cagacatatt ggcctcgtgc caggctccct
     9241 ggcccatagc gctaacccca gggagaatgc gtggtgttac tttggctggt agccgaactt
     9301 cgccacgatg gttaaacacc cgcaccatat cgccggtggc aatcccacgt ttctgtgcat
     9361 ctatagggtt gatccacact tcctgacggc aggctgcctt caggacatca atgttgccgt
     9421 aggtcgagtg agtacgggat ttgtaatgga aaccaaacag ttgcagtggg aaggtactac
     9481 gttcagggga gtcccagcct tcaaaggttg aggcataaac tggcagtggg cttatcactt
     9541 catctttttc cagttcccag gtacgggcaa tttccgccag cttgctggaa taaatttcaa
     9601 tcttaccgga aggcgtttta agtggatttg cctcggggtc gtcacgaaat gctttgtagg
     9661 cgacaaaatg accattggga tctttacgct tatagatacc catttttttc agttcgtcgt
     9721 aagacggtaa cgccggatct ttggcaagca ttttggcgta cagatgttgt aaccattgtt
     9781 cctgcgtgcg accttcagtg aacttttgat agacgtcagg tccaagacgt ttcgcgactt
     9841 cactcaggat ccagtaaatc ggtttgcgtt cgaatttttc gctggtgaca ggctggagga
     9901 aaatgagata tcccatgtta ccggcgtagt cgttaggaat aatatcttcc tgctcaacgg
     9961 tcatcaggtc tggcagcaga atgtcggcat
//
LOCUS       DAZPJP010000142         9949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPJP010000142 DAZPJP010000000
VERSION     DAZPJP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.142"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(124..714)
                     /locus_tag="ACHU9J_003163"
     CDS             complement(124..714)
                     /locus_tag="ACHU9J_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1906632.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRIADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            complement(752..961)
                     /gene="hha"
                     /locus_tag="ACHU9J_003164"
     CDS             complement(752..961)
                     /gene="hha"
                     /locus_tag="ACHU9J_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1906633.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRNSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(1007..1468)
                     /locus_tag="ACHU9J_003165"
     CDS             complement(1007..1468)
                     /locus_tag="ACHU9J_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR1906634.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(1713..1925)
                     /locus_tag="ACHU9J_003166"
     CDS             complement(1713..1925)
                     /locus_tag="ACHU9J_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1906635.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(2054..2614)
                     /locus_tag="ACHU9J_003167"
     CDS             complement(2054..2614)
                     /locus_tag="ACHU9J_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1906636.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(2717..3577)
                     /locus_tag="ACHU9J_003168"
     CDS             complement(2717..3577)
                     /locus_tag="ACHU9J_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1906637.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(3636..4382)
                     /gene="traX"
                     /locus_tag="ACHU9J_003169"
     CDS             complement(3636..4382)
                     /gene="traX"
                     /locus_tag="ACHU9J_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR1906638.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWFQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(4402..9672)
                     /gene="traI"
                     /locus_tag="ACHU9J_003170"
     CDS             complement(4402..9672)
                     /gene="traI"
                     /locus_tag="ACHU9J_003170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR1906639.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHRQEAKLAEAQQKSMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPKAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRSAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(9672..>9949)
                     /locus_tag="ACHU9J_003171"
     CDS             complement(9672..>9949)
                     /locus_tag="ACHU9J_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR1906640.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         2069 a         3112 c         2343 g         2425 t
ORIGIN      
        1 tactgtcgtt taatgtgatt taaactgtga aatagtatgg ttttcagtta tggaaacgcc
       61 gggagcgggg aaaacttgct ttttcccgtt tccggggttg gacaactgag caacgcgaag
      121 gcgtcagcta cgatgttccg gtgactgctg agcagcccct gttgtattca gccattctcc
      181 ggtcatctgc agcagttttc tggcatcgtg gcttctgagc aacggaatac cagcctgcct
      241 cagaacctct tccagaagaa tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc
      301 atccagctct actgctgcaa caatactgaa cgaacgccgc tcaacgataa ccacatcaac
      361 atgccactga gacaccatcc tgaataactg ccaccactgg cgtgatcgtg gccggatatt
      421 cccgttcagc tggacgatat ccgcgatccg tacctgcgga cacagatacc accgcttcat
      481 atccacaatt ctgaacagtc ctttcataaa actgacttcc ctttctgtca gcagcgtctt
      541 ctgacgttgc cagtattcac cgccggacag tattcgctct gcatcaccgg tccgtatacc
      601 tgccgcatgc aattgcatca gcaaaggaga atgctgattt acacttctga ggataatccg
      661 gataagtaac gctgcaataa tcagaacaat aagaataaaa ataatcagct tcattgttta
      721 tctgctcctg gtaattaatt atgctgaatt attaccttac atatttccat atctcttttg
      781 gtatcctgtc atataacttt ccggtgatta attccgctaa ccggtggtct gctgctgaat
      841 taaatgattc acgttcactg tttaataaac tgtttctgtt tttatgaata attttttcca
      901 gcgtattgac tgatgaacat cgccgtaact gatacagcca ttcctgtttt gttttcgcca
      961 tttttattcc cgctgagaac agatgaatga taagattgcc atcgctttat ttacggtgtc
     1021 tccatatcca gggaggaacc ggatcagcat ctgaccagag cccacgtttt tgttgtctgg
     1081 cttcgttctg aaggacaggc agcacgggat ctgtgttgta ctgctcatat acccaggcag
     1141 ccccggcccg gaccataaac tgattaacat tcattccgtc tggtgcataa acctgcccga
     1201 gaatacgacc ataacgatct cgctgaaaat atgtgacggt aaccgttttt cctgcaacca
     1261 gtgatttcat catgtccgtt gaccaccggc cataatcctg tttcttttcc ggtgcatcaa
     1321 tatttatcag cctgatccgc actgccttcc gtgagtccat gacctcgatg gtatcaccgt
     1381 ccagtacacg aacaacccgt ccatgaatat ctgcacacaa taccggccat gaaaagataa
     1441 ataataccag tggaatatat ttcctcatgt tattctccat atcagataaa tggcacctgt
     1501 atttcaggta atcctgaata tcgcagcaca cactgaataa caccttattt tgtgaaatta
     1561 atcacaaaat ataaaatcac gacttaatat ggaaaaaact ctgtgctact cagaactgtc
     1621 cggtcatact tcccgaccgg atattaaaaa aagcaaatct catgcattat tcagccttct
     1681 gaatccctgg agtaatgaag gctgcttttt cgttacaccc ttccccggca gcgccggtgg
     1741 cagtgttcag ccctttttcg ggcaaactgc tgccagtctg tccggggagc agactgcgca
     1801 gcccgtagcc atgcctcagc ggctcccggc cagtcccccc tgacttccag ccggacagcc
     1861 cggcgtccga cctgccggta gcgccatacc ccggagtttt gcgaatcacg ccctgttccg
     1921 ttcattttcc ttcccccttt aatccaggcc ggtgaatgtg ttataaattt ttataacctt
     1981 cttctgccgg gtatggaaga aggtttcaga gaagccttat ttttccgcct cctttagtgt
     2041 gaaggaggct tttttattgt tcatcaagca cggcctgaag ttctgccttt atccggttct
     2101 ggcggcggat tttatccaga cgctctgccg catacgcttc ttcctcctga gaaatatgct
     2161 ccgtcacata cccttccgtg tcataccggc aggcaccggc tttcatggca cacagatagc
     2221 tttctgaacg ggtgatggcc ttcagcgccc tgcgcagttt tttatgcgag agcgggatat
     2281 tccgctgcgc cacgtcttcc agtaacacgt cccggatacc gcaggccaga agtcggggtg
     2341 tgtcaccgtc aaataatccc ggccaccagg gtttcagggt gttcacggcc tcatccagcg
     2401 agggcaggtt cagataaatg gacagcgcct gtctggcctg cgcttttttt gctgtcagct
     2461 ctgcttcccg ggcagccttc tccgccagtt tctgcttttt caccttccat tttggtggcg
     2521 tggtgacatt gatgatggtt ttccggctcc gggtcggtgt ttccccttcc gttttccgtt
     2581 tcagtgtcag taccggtcgt ttctgctctg tcatgattgc cctgaccttt gtgaaaatat
     2641 gtcctccagg tcgttgaaac tactcactat gatattaagc tgacctcaac gacctctgac
     2701 atattaatac cgacaattaa agatgttttt taaaccattg cgtctgtact gccgccacac
     2761 gttcaaaaaa cgcgccttca taaatatcat aatggcatgc gtcagcttcc tcgtataatt
     2821 ccttagtacc agaagcaaca gcatcatata aggcttttcc ctgttctggt ggattaacac
     2881 tatcctggcc ggcaataaca atcaaaactg ggcactgtac tttggctgcg ctctctgccg
     2941 gtttatattg caacgtctcc ataaccgtaa ggaaaggtat ttttatatcc atttcaggat
     3001 actgaccttt taccttctcg aagaatacct ttgattcatt atcactcagt acccgggtaa
     3061 cccccacaaa catctctttc ccggtgtttt tcttcttctc tgccatttta ttcagcgtgg
     3121 acagaaaaga cgctctctcc gattcattca tctctccggt aaccagaaca tcaccgtcag
     3181 caaaagccag ctggctgaca atacatttta cgcgttggtc ctgcgcagct gcactgaaga
     3241 catgcccccc tcccagcgat gttccccata acccaatacg ctgattatca atacattcct
     3301 gcttttctgc ccaattgata actgaaataa tatcttcggt ctgcattgca ggaaccagac
     3361 gccctcgttc tccatcactc tcaccgaatc ctcgataatc aaaagtaatc gttgcgaatc
     3421 ctgcttctgt gaaggcattc gcaaagcaag ggagcagaac attcctgata ccgcaaaacc
     3481 catggcataa gataatcaaa ggatgtttta tatttccttc aggaacacga aatgtcaggg
     3541 caataccttc agagagtttg tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt
     3601 attaataatc agaatcagcg tcagtatcat accagtcaca acgccagaac gcccagcaca
     3661 gccagatgac aggcgtagaa taccgggaaa aaatccccgg gccagaaacg gggtaatgat
     3721 tttcctgcac acgacaccag accaacggtc agcaccgtca ttacaagacc ggccaccgcc
     3781 gctgcatcac tggaggcaag gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc
     3841 gccagacgtt ccgctctgtc ttccgcccgg tacagtcggt aactgaccgc cagcatcagc
     3901 agcccggcaa tgccgtaact ggtgccggac aagggccccc acagggccat cagaagaatg
     3961 gccgctgctg tacgccaccc actgcgcgtt tcacaccacg tcagcacctg tgccgccact
     4021 gcaaaggcaa acaggatatt cccctcatac cagggaaagc ctgccagata atatgcgaac
     4081 tgggcaataa ttccccatcc ccacagccgg ttaatggccg gttgccggat atgcgcatga
     4141 cgggacagat tcagccccca caccagggca aacagcggaa aggctcctcg cccggccaga
     4201 aacatccatt cctgcttcag ctggaatatc agattgatgt ggtccagtac catcagcacc
     4261 agtgccaccg ttttgatgat atcccgctgt ccgggtgacc agaccagaaa tgactgaaac
     4321 caggtatcgg tccgtgcagc cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc
     4381 atcagtgttt atcttcctgt atcagtctcc gcccagggtt ttctctttct gcaggtcacg
     4441 gaccatatcc cgctccatct gctgcagacg ctcctgcagc agattttccc gggcaacctc
     4501 ccggaccgct tcccgctccc gttgtgattc acgcagcaca ctctccggca gcgcggcttc
     4561 ccgttcagtt atggcagacc ggtcacgctc ctgtccggca atctccctga cagcctgctc
     4621 tgttttgccg tccggcaggt caggtttgtt ctctgccatt ttacggacaa tttcatctgc
     4681 gcggcgttcc gtttcacggc ggatggcctc ttcagcctgc cgctgtgcca gcacttcagc
     4741 tgtaaccggt tcaccatttc cggcccccgg ctggacgctg ttgtccggga tatcccccca
     4801 cacgcgacca ccggtgatgg caccgggatt ccacggacga ccttcaccgg caattcttac
     4861 caccacaccg ctgtcgggat tatcacgggc aatccggaca ccctcctgca tattatcagc
     4921 cagcaggctt tctccgttac ggctgccctg cagggccacg aactgcgcat ccccgctgcc
     4981 ttttacccga ccttcaccgc tgaatccccg cagcccgttt ccgtcatccg tggtcagcgg
     5041 gttcagccag ataccggcgg acctgccgtt acggtcaaac gccggcagtg ccacatacgg
     5101 ttgcggatat ttacgtcccg gagcaataaa ccgtgcagga ctgtctcccc cggccagccc
     5161 tgcctgacgg agaacggcac ggcctgccgc cacgtcccgc agttcccgtg ccgtactgaa
     5221 cagccgctgc gcattcatga cctcccggtc cggtttcggc tccagcacat cgtgggcagt
     5281 tcctttctgt acggcattgt taatggcatc cgtccagccc tgacggttat cggtgtacac
     5341 ctgcacatgc tgcttcatac gcgacagggc cacgtaggct gactcaaagc cggccatctg
     5401 tttccggtta ccttccgtgc cttcaagcgc aatggcaaag gtttcacttg ccccctgcgc
     5461 accgtgggcg gtgatggcat aggccaggtc aatatgttgc tctgcccgct cctgaccggg
     5521 gcgaatcacc cgggtctgct gtccgtccga cagcgtgaca ctgtcaccgg aaaccgctgt
     5581 caccgtccag acgctgttgg ccacataacc gcgctcccgg tcactcttcg tgaagcgcat
     5641 ccggtcaccg gttcccaccc ggatggtgtc cggagtgtac agtgtgacgc cttcagccac
     5701 cgcctcccgg ggcgaaatca gccgcgtgtt accttccgca tcctgcaggg ttatcagccc
     5761 gtcatccttg ctgataccgg caatccggtg atacacatta tccaccaggg caagtgcgtc
     5821 ccgatgtgtc tcccaggtgg agagacgacg caactccccg tcgcgtatat tcgctgtgtt
     5881 caggacaggc accatgacct gctcctgccc cagctcaccg gccttttccc gtgcatcatg
     5941 aatcatgctg ttcagcacgc gccggtcctc attcaggtgc gtgacaataa gcgtctgctc
     6001 ccgtgctttc ggtgtcctgc cggtatagtc gcgaacaatg gcttcataca gtgtcatggg
     6061 gacatccggg aaagcctcgc ctttcagcat cgacttctgc tgcgcttctg ccagtttcgc
     6121 ttcctgtctg tgactgaact ccgtcacgga gtgctccggt gcccatgcgc cctccagacg
     6181 tggcacctga gacggtttca cactctcaag cccggacagt gccctttcca catcccggtt
     6241 aatcaggctg tataccgcct cccgcagttc cggcgtctga cgcacaatct ccttcatgat
     6301 ggccacatcg gcagcactgc gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc
     6361 ctgcagctgg tccgtgtcac cgctggccac agcacgaccg ccaccggccg caatcagggc
     6421 gtatgcccgt gccatgtcgg tattgcccac cattgagctc tcatcgagca gaaacagcgt
     6481 gttgctgaaa tccggcgttt ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga
     6541 cgccagtgtc tgtgcatcca cgccggcgct gcgcatctca ccgaccgcac ggtgcgtggg
     6601 ccccagcccc acaacccggg gacgctcact ctccggcagc atgttcacgg ctgacatcac
     6661 cgcccggaac tgagtggtct tacccacacc ggcatacccc tgtaccaccg tgaaacggtc
     6721 ggacgtttcc agtatcatac gggtggcggc acgctgtccc gacgttaacg tctccatgag
     6781 ttcgccgggt actctctcca tcagcggcgt gaccgcctcc ttgccttcga gaatatggcg
     6841 aagaatgctc ttttctgcct catacgacgc acgggaaacc agcaggcctg tgccatagcc
     6901 ttttgccaca tccacataca gtaagtcacc gcgttttatc tgcgcattga tttcccctcc
     6961 cagttcagta aaaccggttc cttctgcagc aaacgacttc gcctctgtca gcaggtccac
     7021 catgctgaag gccaggtttt tactttccag cacaggaagg gccagatgaa tggcctgctg
     7081 tgccggcgtg tgaagcccgg ctttctgcag actgatagcg gtttccagca atgtctcacc
     7141 ggcacgcgcc tttatctgct cagaaaccac cgtaaaggac ggatggcggg cgagtttttc
     7201 cgcagtacgg gtttcatcca gtgaggaata cagccggaca tcacgaccgc tgcgggccag
     7261 accgttcaga gtggcgttgt ccattgccat ctgtgtgacg gaggcaaaca ccgtcgcact
     7321 gtcactgacg gaatgcccgg gcgtttccac ccagccgttc tccagcttca gtgccgtgaa
     7381 cggtgaatca gccacaggca gggtggccgg ttcagcccgc cccggcacaa caaccgtcat
     7441 cgcatcttca ctgacggatg ccacctgcag gcggtcaccg ccggagacgc gaagtccggg
     7501 aattttccct gtcaccctca gtcgctcgcc gtctgccacc ggcatttttt ccggccggaa
     7561 cagcgaccag ctgctgtcca gggaggaaat acgcaccacc tgcgtttcgc cctgcgcatc
     7621 ccgcagggtc aggctgtgac tctgcgccgt cacccggtcg ataacatagc ggtcatgact
     7681 gcgtgtctcc gggttccact gctccatcac catccccggt cggtacatat cccgcagata
     7741 acggctccgg ctgtccagcc agaccggtga cagggcggtc atggtcacct ccgggtgtcc
     7801 gagcacgccc tgtgttttca gctcactgcg aatggcctgt gtcagtatag cctgttcccg
     7861 taccccgctg acctgtgcca cgctctcttc tccggctttc acgctggccg caaaatctcc
     7921 ggccagccgg tcatagcgga cattccggtc cggttcactg atgatggttg ccggtcgctg
     7981 ttctccgccc tgccagcgat acgtgttcac cccggcatcc ttcatggcca tcagcgcact
     8041 gcctgtaccg gttcgctgcc cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc
     8101 accgtccagc agggttaacg tctctttcag ggagagtttt tcgccctggt caacgataac
     8161 ggtactgccc ggtgtgaagg ccataccctc cagcagctga cgacgtccgg ttatcagctc
     8221 accggacagc cgttcatcct gcttcaggtt catctgcgag cgacggtcag cagcgataat
     8281 ctgcacctcc cgcccctgct cccgggccat catgaccagt tcagccaccc gctcacgctg
     8341 cccggctgca ccgccctgcc cggacacaat ggccagcgac ggacggtcct gtgccagaac
     8401 actgaccgca tcgctgtaac cagccgtccg ggggacactt ttctccggat gtacggtcac
     8461 ccggttctgt ttcatgatgt cacgactgag tgcccggacg gacagctcat cgagcacatg
     8521 aatcccggat gtgaacagcc ccttctcacg gtcgaggggg ataagctgct cacggctgat
     8581 ggcctcatcg ataccggcgc gtgcccgttc aatcacacca ttttccggcg gcagtatgcc
     8641 gaccgtcctg gccagcacgt ccgtatacgt gaactgcact tttcgttcac ttaatccggc
     8701 aatcgcctgt gtcaccgcct gctgcacatc cggcccgtcc tgtgaagcag gcccgggcgc
     8761 ctgcgtgcgg atctccgcac gctgatccgc cgcgtcacga tatgcccgga tgtcgaaccc
     8821 ggtttccttc agcgtctgca tccattcagc cattctgatt tccggatcga cgtgctgttt
     8881 ggattttcgc gtatccagtg ccgccacatc ccgggatttc agcgaggcgt cttcaccgac
     8941 ggcctccctg atagcctgtg aacgactgga aaaggcctcc accggtacgc ccggcatctc
     9001 ccacataccg tgcttaccga ccacttcggt ttcataaccc agcgcctcaa cgcgctgacg
     9061 taactcactc tgatatattt tcccgaacgc aatccggttc gctagcacgt tctcggaaaa
     9121 tccggttttc cccactttgt cactgctcag tgtcttccac tcgccgttat gctgcgtgac
     9181 attagccacc actacatgcg tgtgtaactg tgggtcctga tcgcgactgg tgtcatggtt
     9241 aaacagtgcc atcaccagat taccggtcag caccgtttct gactgtccgt ccgtcatcac
     9301 ccgtgtggaa gccagcgcct ccacctgacg gacagcaaaa tccacggcct ggttatgtgc
     9361 atcaataaga cgtttatccc ccccgagcat ggccatcatg gagacacttt tgggggcgga
     9421 gaaggtcagg tcgtagccgg gacgatgctt attactgcca tcctgcatgc ggcttaaatc
     9481 cgccccgtcc ggcagcctgc cctccagaag acgggtaaaa acatccttat cgacgctgcc
     9541 ctgcagccca agctgttcag ccccctgacc agcccagcgt tctcccatgc tgcccagcac
     9601 atagtaatta tccttgtcgg tgtaatagtt tccggcactg cctgccgatt taacaacgct
     9661 gaaactcaac atcagaaatc atctcccggc tcaacatcct ccccgcgctc ccggtgcaca
     9721 ttaatgttca cctcttcacg acgctgcata tgctgctgga tgtccggatg attttcctgt
     9781 tgccatgcct cataagcggc catatccacc acttcaccgg attcactgat cccgggtggc
     9841 agttgctgtt ccatctcttc ttccggtttc attttcagct cctgctcgat cccccctgcc
     9901 ggaacactca cacctgcatc tgacttctta tcattgatga cagaagaca
//
LOCUS       DAZPJP010000143         9842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPJP010000143 DAZPJP010000000
VERSION     DAZPJP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.143"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            2..574
                     /gene="lpfA-O113"
                     /locus_tag="ACHU9J_003172"
     CDS             2..574
                     /gene="lpfA-O113"
                     /locus_tag="ACHU9J_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1906641.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            622..1353
                     /gene="lpfB-O113"
                     /locus_tag="ACHU9J_003173"
     CDS             622..1353
                     /gene="lpfB-O113"
                     /locus_tag="ACHU9J_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1906642.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1378..3900
                     /gene="lpfC-O113"
                     /locus_tag="ACHU9J_003174"
     CDS             1378..3900
                     /gene="lpfC-O113"
                     /locus_tag="ACHU9J_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1906643.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3911..4984
                     /gene="lpfD-O113"
                     /locus_tag="ACHU9J_003175"
     CDS             3911..4984
                     /gene="lpfD-O113"
                     /locus_tag="ACHU9J_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1906644.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5232..6272
                     /gene="pstS"
                     /locus_tag="ACHU9J_003176"
     CDS             5232..6272
                     /gene="pstS"
                     /locus_tag="ACHU9J_003176"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1906645.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6359..7318
                     /gene="pstC"
                     /locus_tag="ACHU9J_003177"
     CDS             6359..7318
                     /gene="pstC"
                     /locus_tag="ACHU9J_003177"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1906646.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7318..8208
                     /gene="pstA"
                     /locus_tag="ACHU9J_003178"
     CDS             7318..8208
                     /gene="pstA"
                     /locus_tag="ACHU9J_003178"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1906647.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8299..9072
                     /gene="pstB"
                     /locus_tag="ACHU9J_003179"
     CDS             8299..9072
                     /gene="pstB"
                     /locus_tag="ACHU9J_003179"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1906648.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9087..9812
                     /gene="phoU"
                     /locus_tag="ACHU9J_003180"
     CDS             9087..9812
                     /gene="phoU"
                     /locus_tag="ACHU9J_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1906649.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2534 a         2261 c         2505 g         2542 t
ORIGIN      
        1 tatgaagcgt aatattatag gcggtgcatt cactctggca tctctaatgc tggccgggca
       61 tgcactggca gaagatggtg ttgttcactt cgtcggtgaa attgtcgaca ctacttgtga
      121 agttacctcc gatacagccg atcaaattgt cccactgggt aaagtcagta aaaatgcatt
      181 ttcaggtgta ggtagtctgg cgtcgccaca gaagttcagt attaaacttg aaaattgccc
      241 ggcaacgtac actcaagcag ccgttcgttt tgatggtaca gaagcgcctg gcggcgacgg
      301 cgacctgaaa gtgggtacgc cgcttacagc aggcaaccct ggtgatttta ccggtacagg
      361 acaagcgatt gcggcaaccg gcgttggtat tcgtattttt aaccagtccg ataattcgct
      421 ggttaaactt tataacgact ctgcttatac cgctatcgat gctgaaggca aggctgaaat
      481 gaagtttatt gcacgctatg tggcaaccaa tgcgaccgta acggctggta cggcgaacgc
      541 tgattcacaa tttactgtcg aatataagaa ataatttatc aagggccttt ttattcagtt
      601 ggctttggcc agggagtcgt tatgtccaga aaaagttgtt taatcaggct ctttaaaaca
      661 acagtttcat gtaagttatt taccgcgatt atactcagcg tttttttagg ccaaccagca
      721 ttaacttatg caggcgttgt cattggtggt acgcgggtgg tttacttaag taataatgct
      781 gataaatcta tttctgtatt tagcaaagaa gaaaaaatac cttatctcat ccaggcttgg
      841 gtagatccat ttaataaaga ggataaaagc aaagcgccgt ttaccgttat tccgccagta
      901 tcacgattag aaccttcgca agaaaaaata ttacgcatta ttcacaccaa aggtgtgtca
      961 ttgcctgatg atcgcgagtc agtcttctgg ctgaatatta aaaatattcc gccatcagcg
     1021 agcaacaaag cgacgaactc gctggaaata gcagtaaaaa cgcgtatcaa gctgttctgg
     1081 cgaccagcaa atatccgcct tattcccgaa gatgctgcgc cgaaagtgaa atggcgccgg
     1141 gaagggcgaa atctgatagc agagaatccc aacccaattc atatcagtgt gatggatgtc
     1201 atcgttgatg gccatgatgt gcccctcaat atgatccgtc cttttgaaac gctcacgtta
     1261 cctttacctg ctaattctgc gggaggccaa atgacctggc gatttattaa tgattacggc
     1321 gcagtcagtg acccgataaa aatgacgctg taagcaaggg ataaggatag gcgtattatg
     1381 atgacgacca gaatagtggt tggcctcacg gcagggacgt gtctgatttt ctcgcaaaac
     1441 ctgatggccg aggtcagtgt attcaatccg gcgcttctgg aaatcgacca tcaatccgga
     1501 gtcgatattc gccagtttaa tcgggcaaac ctgatgcccc caggtgttta tagcgttgat
     1561 atttttatca acggtaaaat gtttgaacgt caggatgtga catttgttca ggataatcca
     1621 gatgctgatc tgcacgcttg ctttattgcc attaaaaaaa cactgtcctc ctttggcata
     1681 aaagttgatg cgctcaaatc gttcaatgat gtggatgaga cggtttgcct cgatcctgct
     1741 ccacgtattg aaggctcatc ctggcagttt gacagtgata aattgcagct gaatatatcc
     1801 attccccaaa tctacatgga cgcgatggct tatgattaca tcagccccac gcgttgggat
     1861 gaggggatta acgcgctcac catcaactac gatttttctg gttcacatac actacgttca
     1921 gattatggtt cacaagagac agataccagt tatctcaatc tgcgcaatgg actgaatatt
     1981 ggaccgtggc ggctacgtaa ttacagtact ttaaacacca gcgatggccg tgcggaatac
     2041 aactccatta gtacctggat acagcgcgat attgccgcgt taagaagcca gattatgatt
     2101 ggtgatacgt ggacggcgag cgatattttc gacagtacgc aaattcgcgg cgcgcgtttg
     2161 tatactgata acgatatgct acccgccagc cagaatggct ttgctcctgt ggttcgtggg
     2221 attgcaaagt ccaacgccac cgtcatcatt cggcagaatg gctacgtgat ttatcagtct
     2281 gccgttccac aaggtgcttt tgagatcacc gatctcaaca ccgccagtac aggtggcgat
     2341 ttggacgtaa ccatcaaaga agaagacggt agcgaacaac gattcaccca accttatgct
     2401 tcattagcga ttcttaaacg tgaaggtcag acagatgttg atgtcagcgt gggtgaattg
     2461 cgcgatgaag acggatttac accggacgtc cttcaggcgc aaatacttca tggtttttcc
     2521 cacgggatca ctttatatgg aggtatgcag gctgctgaaa attatggttc tgcagctctg
     2581 ggtgtcggta aagatcttgg cgctttgggc gcaatttctt tcgatgtgac acatgctcgt
     2641 gcgaatttta gccatgatga tacagaaacg ggtcagtcat atcgctttct ctattcaaaa
     2701 cgatttgacg acacagacac tagcttgcgc ttggttggct atcgttactc caccgagggc
     2761 tactataccc tcaatgagtg ggcatcgcgg cgcaacagcc ctgaagactt ttgggaaaca
     2821 ggtaaccgac gtagttgcgt ggaggggacg ctaacgcagt cgttggggag agattatggc
     2881 aatttatacc tgacattaag ccggcaacaa tactggcata ccgatgatgt cgaacgatta
     2941 atgcaatttg gctacagcag tagctggaag cgtctctcgt ggaacgtctc ctggagttat
     3001 tccaatactg ccagacaggg gacggggaac aaccatgcca gtgataacac cagtgagcag
     3061 atctacatgc tctctttatc tgttccttta ttgggctggt ggggtaatag ttacgccacc
     3121 tattctgttt cgcaaaacga taattccggt agctcacatc aactcggact cagcggtacg
     3181 gcgctggaaa gaaataacct ttcatggaat ttaatgcagt cctataacag tcatgatgat
     3241 gaggttggcg gtaatatgtc cctgacctat gatggctctt atggcacggt gaacggcagc
     3301 tataactaca gccaaaattc ccagaggctg aattatggta tcagaggggg aattctggca
     3361 cacagcgaag gggtaacgtt aagtcaggag ttaggtgaaa ctattgctct tgttaaagca
     3421 cctggggccg ccgggttaga aatagataat atgcgcggtg ctgcgacgga ctggcgcggc
     3481 tatacggtca agacacagct aaacccttat gatgaaaatc gggtagcaat cagcgataac
     3541 tatttctcga agtcgaatat agaacttgat aataccgtcg ttacgatggt tcccacgcgt
     3601 ggtgcagtgg ttaaagcgga gtttgtgact catgtgggtt atcgcgttct cttcagagtg
     3661 ttaaatgcaa atggtaaacc ggtacctttt ggagccattg ctgcgataca agatgcaagt
     3721 ttggcagatt caggaattgt cggtgaccgt ggcgaacttt atctttctgg tctaccagaa
     3781 aaaggacagg ttacgttatc ctggggagaa aacgcctcaa caaaatgcat cttcaattat
     3841 tcactttcga caccagaaag tgagagcgga ttaattgaac agggtgtgac atgtcattaa
     3901 aaggattttt atgaacaagt atatcaaaca gtggtgcttt gctgtgttta tgctctcgtt
     3961 aagtagtgta gcacttgcag ctcctaaagg tatctgtacc cctgataatg gcgtatttca
     4021 ccgtacgctt gatttctcgg gatatcttat taccgcgaac gaaaataagg ttggaacgac
     4081 atttaatacc actgtaacta atggatcctc atatcctgga cgctgtcatt gtgatactgg
     4141 taatgtagga gaattcccct atatctatta tacgtcaaaa ataaaccagg cattaactta
     4201 tgctggggtt cactctaata ttaattatta tgatcttaat ccgaaccttg atgttgggat
     4261 tgctatagat attcttgggg ttgggtatgt gaatgcacct tttgaatatc atgctaacaa
     4321 cccgtctggt aatacaaagt acaattgcaa ccgcattgaa cctttaagta tatccagtgg
     4381 gggtaaagcg atagtatatt tttatatcaa gaagacattt gcaggaaaat tgattattcc
     4441 tgaaacaaag atagtgacat tgtatggaac aattagccgt gacaccccgg tggattactc
     4501 acagccgatg gctgatgttt atattcgggg cgatattaca gctccgcaaa gttgcgaaat
     4561 aaataattta cagccagttt attttgattt taaagaaata ccagctgcag atttttcatc
     4621 tgttgttgga agtgcggtaa caacacataa aattactaaa acagtcacta ttgagtgtga
     4681 aaacctgggg atactgaata ccgatgatat cagtacgtct ttttatgcta ccgaacccaa
     4741 tacggacaat tcaatggtcg taacttcaaa ctctaacgtg gggataaaaa tttacgacaa
     4801 aaataataag gaaatcaaag tgaacggtgg tgagttgcca acagacatgg gtaaatcaac
     4861 agtgtatggt gaaaaatcag gtagtgtaac tttttcagct gctcctgcga gccttactgg
     4921 agctcgccca gccccggggc aatttactgc aacagcgacg ataactgttg agattgtgcg
     4981 ctaataggtc tgacaacgat tgttcatcac atcgttgtca tctcacccat ttctttatca
     5041 cttttaaatg catcatgaca aaaagatgac atgtgggatt ttcatcttca catttaaaat
     5101 cagtaagtta tttgttaatt cctttctgtc ataaaaatgt catattcctt acatataact
     5161 gtcacctgtt tgtcctattt tgcttctcgt agccaacaaa caatgcttta tgaatcctcc
     5221 caggagacat tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat
     5281 cgatgagcgc tttctctgtg tttgcagaag caagcctgac aggtgcaggt gcaaccttcc
     5341 ctgcgccggt gtatgccaaa tgggctgaca cttaccagaa agaaatcggt aataaagtta
     5401 actaccaggg tatcggttct tccggtggcg taaaacagat tatcgctaat accgttgatt
     5461 ttggtgcctc tgacgcgccg ctgtctgacg aaaaactggc tcaggaaggt ctgttccagt
     5521 tcccgaccgt gattggcggc gtggtgctgg cggttaacat tccagggctg aagtctggcg
     5581 aactggtgct ggatggtaaa accctcggcg acatctacct gggcaaaatc aagaagtggg
     5641 atgatgaaac catcgccaaa ctgaatccgg gtctgaaact gccttcacaa aacattgctg
     5701 tagtacgccg cgcagatggc tccgggactt ccttcgtctt caccagctac ctggcgaaag
     5761 tgaacgaaga gtggaaaaac aacgttggta ctggctctac cgtaaaatgg ccgatcggtc
     5821 tgggcggtaa aggtaacgac ggtatcgccg cgttcgttca gcgtctgccg ggtgcaattg
     5881 gttatgttga atatgcttac gcgaagcaga acaacctggc gtacaccaaa ctgatctccg
     5941 ctgatggtaa accggttagt ccgaccgaag aaaacttcgc taatgcagca aaaggtgcag
     6001 actggagcaa aaccttcgct caggatctga ccaaccagaa aggcgaagat gcatggccta
     6061 ttacctctac cacgttcatt ctgatccaca aagatcagaa gaaaccagaa caaggcacag
     6121 aagtgctgaa attcttcgac tgggcgtaca aaaccggggc taaacaggcg aacgacctgg
     6181 attacgccag cctgccggat agtgtagttg aacaggttcg cgctgcgtgg aagaccaata
     6241 ttaaagacag tagcggtaag ccgctgtact aataaaactc taggccgggt acggtgtttt
     6301 acgccgcatc cggcattaca aaatgacttt gtaaacgcgt ttaactgaag agtaacttat
     6361 ggctgcaacc aagcctgctt ttaacccacc gggtaaaaag ggcgacataa ttttcagcgt
     6421 gctggtaaaa ctggcggcgc tgattgtgct attgatgttg ggtggcatta ttgtctctct
     6481 gatcatctcc tcctggccga gcattcagaa atttggtctg gctttcctat ggaccaaaga
     6541 gtgggatgca ccgaacgata tctacggggc gctggtgccg atctacggta cgttggtgac
     6601 ttcgtttatc gcgctgctga tcgccgtccc ggtgagtttc ggtatcgccc tgttcctgac
     6661 tgagcttgcg cctggctggc tgaaacgccc gctgggtatc gccattgagc tgctggcagc
     6721 cattccaagt atcgtttacg gcatgtgggg cctgtttatc tttgcgccgc tgttcgccgt
     6781 ttactttcag gagccggtcg gcaatatcat gtcgaatatc ccgattgttg gcgcgctgtt
     6841 ctctggcccc gcatttggta tcggtatcct cgcggcaggc gtgatcctcg ccatcatgat
     6901 tattccgtac attgcggcgg taatgcgtga tgtgttcgaa caaaccccgg tgatgatgaa
     6961 agagtcggcc tacggtattg gctgcaccac ctgggaagtt atctggcgta tcgttcttcc
     7021 gttcaccaaa aatggtgtta tcggcggcat catgctgggg ctgggccgcg cgctcggtga
     7081 aaccatggcg gtgaccttta tcatcggtaa cacctaccag ctcgacagcg cctcgctgta
     7141 catgccgggc aatagtatca cctctgcgtt ggcgaacgaa tttgcggaag cggaatccgg
     7201 tctgcacgtt gccgcactga tggaactggg cctgatcctg tttgtgatta ccttcatcgt
     7261 cctcgccgca tcgaagttta tgattatgcg cctggctaag aatgaggggg cacgctaatg
     7321 gctatggttg aaatgcaaac cactgcggcg ctggctgaat ctcgccgcaa aatgcaggcg
     7381 cgtcgccgcc tcaaaaaccg tattgcgctg acgctctcga tggcgacgat ggccttcggc
     7441 ctgttctggc tgatctggat tttaatgtcc accatcactc gcggtatcga cggtatgtcg
     7501 ctggcgctgt tcactgaaat gacgccgccg cccaatacgg aaggtggtgg tctggcgaac
     7561 gccctggcgg gtagcgggct gttaattttg tgggccacgg tattcggtac gccgctgggc
     7621 attatggcgg ggatttatct ggcggaatat ggtcgtaaat cctggctggc agaagtgatt
     7681 cgcttcatta acgacattct gctctccgcg ccgtcgattg tggttggtct gtttgtctac
     7741 accatcgtgg tggcacagat ggagcacttc tccggctggg cgggcgtaat tgcgctggcg
     7801 ctgttgcagg tgccgatcgt tatccgcacc accgagaaca tgctgaaact ggtgccggac
     7861 agcctgcgtg aagcggctta tgcgctgggt acaccgaagt ggaagatgat ctccgcgatt
     7921 acgctgaaag cgtcggtgtc cgggattatg accggtatcc tgctggcgat tgcccgtatt
     7981 gccggtgaaa ccgcgccgct gctgtttacc gcgctctcca accagttctg gagcacggac
     8041 atgatgcagc cgatcgccaa cctgccggtg acgatcttta agtttgcgat gagcccgttt
     8101 gcggaatggc aacaactggc ctgggccggg gtattgatca ttaccctgtg cgtcctgctg
     8161 ctgaacattc tggcgcgcgt tgtttttgcg aagaataaac acggttaaca ctttgccgga
     8221 tgcggcgtga acgccttacc cggcctacgg taagcctgat tagcgaaaag catcaggtac
     8281 gatgaggaaa agattgcaat gagtatggtt gaaactgccc cgagtaaaat tcaggttcgt
     8341 aatttgaact tctactacgg caaattccat gccctgaaaa acatcaacct ggatatcgct
     8401 aaaaaccagg taacggcgtt tatcgggccg tccggctgcg gtaaatcgac gctgctgcgt
     8461 accttcaaca aaatgtttga actgtatccg gagcagcgcg cagaaggtga aattctgctt
     8521 gatggcgaca acatcctgac caactctcag gatatcgcac tgctgcgtgc gaaagtgggc
     8581 atggtgttcc agaaaccgac gccgttcccg atgtccatct acgacaacat cgcttttggt
     8641 gttcgtctgt ttgagaagct ctcccgtgcc gacatggacg agcgcgtgca gtgggcattg
     8701 accaaagccg cattgtggaa cgaaaccaaa gataaattgc accagagcgg ttactctctc
     8761 tctggtggtc agcaacagcg tctgtgtatt gcgcgtggta tcgccattcg cccggaagtg
     8821 ctgctgctcg acgaaccgtg ttcggcgctc gaccccatct ctaccgggcg tattgaagag
     8881 ctgatcaccg aactgaagca ggattacacc gtggtgatcg ttacccacaa catgcagcag
     8941 gctgcgcgtt gttccgacca cacggcgttt atgtacctgg gcgaattgat tgagttcagc
     9001 aacacggacg atctgttcac caagccagcg aagaaacaaa cagaagacta catcaccggt
     9061 cgttacggtt gattcaggag tgcgttatgg acagtctcaa tcttaataaa catatttccg
     9121 gccagttcaa cgccgaactg gaaagtatcc gcacgcaggt gatgaccatg ggcggcatgg
     9181 tggagcagca gctttctgat gcaatcaccg cgatgcataa ccaggacagc gatctggcga
     9241 agcgcgtgat cgaaggcgac aagaacgtca acatgatgga agtggcgatc gatgaagcct
     9301 gcgtgcgcat tatcgccaaa cgtcagccga cggcgagcga cctgcgactg gttatggtga
     9361 tcagtaaaac cattgccgag ctggagcgta ttggcgacgt ggcggacaaa atctgccgta
     9421 ctgcgctgga gaaattctcc cagcagcatc agccgttgct ggtaagtctg gagtcgctgg
     9481 gccgtcatac catccagatg ctgcacgacg tgctggacgc gttcgcgcgg atggacattg
     9541 acgaagcggt acgtatttat cgtgaagata aaaaagtcga tcaggaatac gaaggtattg
     9601 ttcgtcaact gatgacctac atgatggaag attcgcgtac cattccgagc gtacttactg
     9661 cgctgttctg cgcgcgttct atcgaacgta ttggcgaccg ctgccagaat atttgtgagt
     9721 ttatcttcta ctacgtgaag gggcaggatt tccgtcatgt aggcggtgat gagctggata
     9781 aactgctggc gggaaaagat agcgacaaat aattcaccag acaaatccca ataacttaat
     9841 ta
//
LOCUS       DAZPJP010000144         9832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPJP010000144 DAZPJP010000000
VERSION     DAZPJP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.144"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..151)
                     /locus_tag="ACHU9J_003181"
     CDS             complement(<1..151)
                     /locus_tag="ACHU9J_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906650.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLN"
     gene            229..1323
                     /gene="ddlA"
                     /locus_tag="ACHU9J_003182"
     CDS             229..1323
                     /gene="ddlA"
                     /locus_tag="ACHU9J_003182"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1906651.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1347..1559)
                     /locus_tag="ACHU9J_003183"
     CDS             complement(1347..1559)
                     /locus_tag="ACHU9J_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1906652.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1819..2127
                     /locus_tag="ACHU9J_003184"
     CDS             1819..2127
                     /locus_tag="ACHU9J_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1906653.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHU9J_003185"
     CDS             complement(2186..3280)
                     /gene="yaiW"
                     /locus_tag="ACHU9J_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1906654.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHU9J_003186"
     CDS             complement(3293..4513)
                     /gene="sbmA"
                     /locus_tag="ACHU9J_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1906655.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4865..6022
                     /gene="ampH"
                     /locus_tag="ACHU9J_003187"
     CDS             4865..6022
                     /gene="ampH"
                     /locus_tag="ACHU9J_003187"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1906656.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="ACHU9J_003188"
     CDS             complement(6023..6646)
                     /gene="iprA"
                     /locus_tag="ACHU9J_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1906657.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6734..9622)
                     /locus_tag="ACHU9J_003189"
     CDS             complement(6734..9622)
                     /locus_tag="ACHU9J_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1906658.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2541 a         2423 c         2361 g         2507 t
ORIGIN      
        1 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
       61 tcatcatttt tgtgatctcg cgcacatctt tgagataacg cactttcttc accagtacgt
      121 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
      181 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      241 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      301 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
      361 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      421 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      481 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      541 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      601 ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa agatgtcacc
      661 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      721 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
      781 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      841 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      901 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
      961 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1021 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     1081 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1141 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     1201 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     1261 cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcattgaa aaccacaatg
     1321 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1381 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1441 gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag aatgaatata
     1501 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1561 gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta
     1621 gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg taactcacaa
     1681 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1741 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1801 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     1861 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     1921 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt
     1981 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2041 attttcgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt ggcgctatgg
     2101 ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatacggcc taaaacgcct
     2161 tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2221 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     2281 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     2341 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc
     2401 gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2461 tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2521 tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2581 aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag
     2641 taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt
     2701 tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac
     2761 cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca
     2821 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     2881 aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt
     2941 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg
     3001 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     3061 ccagcgtcgg tgccagcccc tgactttcaa aggtgatggc gagatctttc gcccaggcat
     3121 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt
     3181 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     3241 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     3301 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3361 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3421 caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac
     3481 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3541 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta
     3601 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     3661 aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca
     3721 gtttgatccc taccactgcc agccatccgg tccccatcag cgaccagacg atagcggcaa
     3781 tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg
     3841 ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca
     3901 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt
     3961 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4021 ggaacacata gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg
     4081 caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg
     4141 aacttagcgc ggtttgaatc aaatcataga acggtgcata ccaggcgttg acggcgaccc
     4201 cgacttccac caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt attgaccagt
     4261 attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa
     4321 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4381 tctgcccgga agcgccggtg atacgcgcca cccagtctcc cccaccggct tgccagaaga
     4441 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgctcccggc tttgggaaaa
     4501 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4561 accgcatgac cactacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4621 gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat
     4681 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4741 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4801 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     4861 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     4921 ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     4981 cgatcatatt ttttacggca gcggtgcaac ggggatggcg ctggttgtta tcgacggtaa
     5041 ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     5101 ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa tgctggtgaa
     5161 attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5221 cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg caacccatac
     5281 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc
     5341 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     5401 tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg cgaatgcgtc
     5461 cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg ggatgaaaga
     5521 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5581 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg
     5641 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     5701 ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat
     5761 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     5821 gggtcgtccg gggattattc agaagacagg cggtggtggc ggtttcatta cctatatggc
     5881 gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     5941 ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg ggaataaacc
     6001 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6061 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga
     6121 cagaacgcga tatgcgtgta cgttgatgga tataattcat cacaccaata cgtgatcgca
     6181 attcttcatt ccagtcaatc attgataata atgttgcgcg gatttgttcg taggaattat
     6241 gcccaatgag ctgcacgtcg cgtaattcca gaattctatt ttgccaggct aaccagtaaa
     6301 aagcgtctcg ccagagttga ttttgttcaa taagcgtaat ggtttgtttg gctggtagat
     6361 gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6421 catcagccag ccccataata taaggggcct gggtaatacc gataagtacg ttttcttctc
     6481 tacgcagaga gataactccc tctaaaataa cgaaagtatc ttcgttattg acatcactgg
     6541 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6601 tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg
     6661 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca gtatttcttc
     6721 ggagtgaaga atattaccag gtatatttaa cacccacgtt cgcggaccag tcttgatcta
     6781 cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga
     6841 actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac
     6901 cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag
     6961 tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac
     7021 gcatgctgtc gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac
     7081 cagactggaa cagaccagaa acgctgccgt aaggagtcac gtaaccagca tcacccagtt
     7141 tgaagtcgta accggctttc aagccgaagc cccaagcgtc ggagttggtg ctaccgtcaa
     7201 cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc
     7261 taccatcaac aaagacgttg ttcgcgaagt gagcagaaga gtagatgtag gcagtctggc
     7321 tgtcttgatc tacctgacca gaacggtcat tcatgtcacc tttagcgaag cctgcagccg
     7381 caccgacgat ccacttagcg ttgttaccgt caactttggt atcaacaccg accatgatgc
     7441 cgttaacatc ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta ccaccgaagt
     7501 agcttaccca tgcgccgccg ttatcagcca ggccatgacg agagttggtc agacgagtac
     7561 caacggtgtc ttgttccagg ttccagatat tggtgttcgc agacgggatg ctcagcgcca
     7621 tgttagcgta gtcggtcagc tccatctgtt gcagaacaac ggtgttaccg cgctgttcag
     7681 cctgataggt gtatgcaccc aggtcagctt tgttagcagc agagaaggtc gcgttggttt
     7741 tggtatcatc gatatagatg agctgcttac ctgcgtaatc agcaatcgca ccagcgccag
     7801 tggagttgtc gatacgaact ttgtagttac cggttgcagc tacaacgttg tctgcagagc
     7861 tgttgtcaac ttcgtcgcca ttgttaatgt cgttgttacc attaacggtc aagtgaccat
     7921 cagagttcat cgcaataaca ccataaccgt agttagcttt gctggtgtcg ttggtacgat
     7981 cgttgacgag gtctgcattc aggatgtaat cattactatg aatgtcaaac acacctgcgt
     8041 aaacattacc gttgttatct ttaacgttgc cactggtcag ttccagagta tcagttgcaa
     8101 atacgctgga gatgtcattc atgttggtgt ttacatccag cacaccaccg ttagtaacgg
     8161 taatggtatt ggcatacagc ggtgcatatg cccaagctga agcagtatct tcaccgaagt
     8221 taacggttga gaaggtatca atggtcaggt gatcggtgtt aacttcaccg ttaacttcca
     8281 ttgaagaacc accgttcaga gtgatggtgt cagaagtcag agaagaatct gcactcacgt
     8341 taacctgaga accgctgttg atcgccaggg tatcgatcag ggaagttttc gtggtttccc
     8401 aatcagaacc gttgttcaga acaacatcga atacgccgct ctggaatgcg ttagcgttat
     8461 caacacgatt ccagccatct tcagagtaag aagcgtacgg gttcaggctg tttggtgcaa
     8521 cgtcgtagaa ggcttcgtca gcaacaacaa cgtcataatc aatattcgct gaaccaaccc
     8581 atttggtgtt gttcagagtc aggttcaggt gatcgacgtt ctgatcgtca gttgcccaac
     8641 cgccatgagt gtaagcagtg gtagagttgt caatcaggtg acccgtcgcg tcaaagttag
     8701 cgttccaggt actgtcaaat acaacgtcac caagcaactg agagttgttg aacgtaacgt
     8761 tgttgaccat acgataatca gaagtgtcat catcgatgaa ggctaacgca acatcatcag
     8821 caggagatgc gccattgccg tagtcacttg gttcaacaga attgccgaaa tgaccacgat
     8881 ctgattgttc gtcttcagtc catgaaccag aagttacagt agagtcatta acaacgatgt
     8941 tgttgtccca ctgatgacct tcggtattgt aggtcgcatt accgaggtcg tgttgaacca
     9001 aagtaatacc tgcaacatgg gagttgttgc tgatgttgat gttactttca acatccagag
     9061 tcacagcggt accaagacca gcggttacca aacgatcata atctgtctta gatgttttac
     9121 cgtctgcatc cagataagta tcggtgaagt agaaggcgtc aaaatcatcg tcaatagtag
     9181 aattagcgat gttcagagtg aagatgtcgc catcaaccca gtcgtggcca gtataatcgc
     9241 taccgttggt ccagacgtag tcgccataac caacctggta agatgtaacc ataccatgaa
     9301 caattgagtt ggagatgttc agtgtattac cggtatctgt gccataatcg tggttcagat
     9361 aataagtaga aattacacca tttacagtgt cgccgctgaa agtggtatca aagtaaccat
     9421 cagctgcgtg gtcatataca taaccaccgt aagtcagata atcgtgatca attttagtgc
     9481 tgtcacccca attgtagatg gtggctgcgt catcgtgata cccgaaggtg ttataagtag
     9541 ctgcattagc ctgagaggtg atagccagag tgcaagccaa tgctaattgt gatactacaa
     9601 gtttcttttt ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat
     9661 caattatcaa tgcatagaaa acatattatg tttcgcgcta acgattatgc tgcgttacag
     9721 acgaatggtt caattattct ttcccctaag tccgaatgtg gacatatata atttatggag
     9781 aaaaacacgc atcaaattga taaaaggacg atatttcaac atgttgcaga ta
//
LOCUS       DAZPJP010000145         9799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPJP010000145 DAZPJP010000000
VERSION     DAZPJP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.145"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHU9J_003190"
     CDS             <1..347
                     /locus_tag="ACHU9J_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1906659.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHU9J_003191"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHU9J_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR1906660.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHU9J_003192"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHU9J_003192"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1906661.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHU9J_003193"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHU9J_003193"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1906662.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHU9J_003194"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHU9J_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1906663.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHU9J_003195"
     CDS             complement(4344..5309)
                     /locus_tag="ACHU9J_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1906664.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHU9J_003196"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHU9J_003196"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1906665.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHU9J_003197"
     CDS             6366..6746
                     /locus_tag="ACHU9J_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1906666.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHU9J_003198"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHU9J_003198"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1906667.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHU9J_003199"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHU9J_003199"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1906668.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2260 a         2484 c         2550 g         2505 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaa
//
LOCUS       DAZPJP010000146         9755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPJP010000146 DAZPJP010000000
VERSION     DAZPJP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.146"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            428..2059
                     /gene="oppA"
                     /locus_tag="ACHU9J_003200"
     CDS             428..2059
                     /gene="oppA"
                     /locus_tag="ACHU9J_003200"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1906669.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2145..3065
                     /gene="oppB"
                     /locus_tag="ACHU9J_003201"
     CDS             2145..3065
                     /gene="oppB"
                     /locus_tag="ACHU9J_003201"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1906670.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYIGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3080..3988
                     /gene="oppC"
                     /locus_tag="ACHU9J_003202"
     CDS             3080..3988
                     /gene="oppC"
                     /locus_tag="ACHU9J_003202"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1906671.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4000..5013
                     /gene="oppD"
                     /locus_tag="ACHU9J_003203"
     CDS             4000..5013
                     /gene="oppD"
                     /locus_tag="ACHU9J_003203"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1906672.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5010..6014
                     /gene="oppF"
                     /locus_tag="ACHU9J_003204"
     CDS             5010..6014
                     /gene="oppF"
                     /locus_tag="ACHU9J_003204"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1906673.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6067..6396)
                     /locus_tag="ACHU9J_003205"
     CDS             complement(6067..6396)
                     /locus_tag="ACHU9J_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1906674.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6431..7891)
                     /gene="cls"
                     /locus_tag="ACHU9J_003206"
     CDS             complement(6431..7891)
                     /gene="cls"
                     /locus_tag="ACHU9J_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1906675.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8034..8207
                     /locus_tag="ACHU9J_003207"
     CDS             8034..8207
                     /locus_tag="ACHU9J_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1906676.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8262..9515)
                     /gene="kch"
                     /locus_tag="ACHU9J_003208"
     CDS             complement(8262..9515)
                     /gene="kch"
                     /locus_tag="ACHU9J_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1906677.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2555 a         2279 c         2421 g         2500 t
ORIGIN      
        1 gatagacatg cttagccaat ctctatttga ttgaattgaa agatgtttgt taaggcatgg
       61 atgcaagcta tagattctga tacggtcaat aaaagagaat tgcttaacaa ttttgcaaaa
      121 tgtattggcg agtaagaacc gcatttggta ctttccgggc aaccgccaga cgattcttta
      181 ttggtaatga gaataattaa caattaaaga gcgtcgcgaa agaataatgt gtctcgacag
      241 gggagacaca gtacgaatcg acataaggtg atcgtctgaa tcaccagaat aaataaagtc
      301 ggtgatagta atacgtaacg ataaagtaac ctgacagcag aaagtctccg agcctgtgca
      361 gggtcccaat ccgggattac acatgctggt taataccagt aattataatg agggagtcca
      421 aaaaacaatg accaacatca ccaagagaag tttagtagca gctggcgttc tggctgcgct
      481 aatggcaggg aatgtcgcgc tggcagctga tgtacccgca ggcgtcacac tggcggaaaa
      541 acaaacactg gtacgtaaca atggttcaga agttcagtca ttagatccgc acaaaattga
      601 aggtgttccg gagtctaata tcagccgaga cctgtttgaa ggcttactgg tcagcgatct
      661 tgacggtcat ccagcacctg gcgtcgctga atcctgggat aataaagacg cgaaagtctg
      721 gaccttccat ttgcgtaaag atgcgaaatg gtctgatagc acgccagtca cagcacaaga
      781 ctttgtgtat agctggcaac gttctgttga tccgaacact gcttctccgt atgccagtta
      841 tctgcaatat gggcatatcg ccggtattga tgaaattctt gaagggaaaa aaccgattac
      901 cgatctcggc gtgaaagcta ttgatgatca cacattagaa gtcaccttaa gtgaacccgt
      961 tccgtacttc tataaattac ttgttcaccc atcaacttca ccggtgccaa aagccgctat
     1021 cgagaaattc ggcgaaaaat ggacccagcc tggtaatatc gtcaccaacg gtgcctatac
     1081 cttaaaagat tgggtcgtaa acgaacgaat cgttcttgaa cgcagcccga cctactggaa
     1141 caacgcgaaa accgttatta accaggtaac ctatttgcct attgcttctg aagttaccga
     1201 tgtcaaccgc taccgtagtg gtgaaatcga catgacgtat aacaacatgc cgatcgaatt
     1261 gttccagaag ctgaaaaaag agatcccgga cgaagttcac gttgatccat acctgtgcac
     1321 ttactattac gaaattaaca accagaaacc gccattcaac gatgtgcgtg tgcgtaccgc
     1381 actgaaacta ggtatggacc gcgatatcat tgttaataaa gtgaaagcgc agggcgacat
     1441 gcccgcctat ggttacactc caccgtatac tgatggcgca aaattgactc agccagaatg
     1501 gtttggctgg agccaggaaa aacgtaacga agaagcgaaa aaactgctgg ctgaagcggg
     1561 ttataccgca gacaaaccgt tgaccatcaa cctgctgtat aacacctctg atctgcataa
     1621 aaagctggcg attgctgcct cttcattgtg gaagaaaaac attggtgtaa acgtcaaact
     1681 ggttaaccag gagtggaaaa cgttcctcga cacccgtcac cagggcactt ttgatgtggc
     1741 ccgtgcaggc tggtgtgcag actacaacga accaacttcc ttcctgaaca ccatgctgtc
     1801 gaacagctcg atgaataccg cgcattataa gagcccggcc tttgacagca ttatggcgga
     1861 aacgctgaaa gtgactgacg aggcgcagcg cacagctctg tacactaaag cagaacaaca
     1921 gctggataag gattcggcca ttgttcctgt ttattactac gtgaatgcgc gtctggtgaa
     1981 accgtgggtt ggtggctata ccggcaaaga tccgctggat aatacctaca cccggaatat
     2041 gtacattgtg aagcactaat ggcaatacgt ggggcaggag tgtcctgctc cacggtgtct
     2101 gatttttatc gcattacaga aggcacaggc cagaaggtag ggcaatgtta aaatttattc
     2161 tacgtcgctg tctggaagcg attccgacgc tatttattct tattactatt tcgttcttta
     2221 tgatgcgcct cgcgccggga agccctttta ccggcgaacg tactttaccg ccggaagtga
     2281 tggccaatat cgaagcgaaa tatcatctta atgatccgat catgacacag tatttcagct
     2341 acctgaaaca actggcgcac ggtgatttcg gtccatcgtt taaatataaa gattattcgg
     2401 tcaatgacct ggttgcatcc agttttcccg tttctgccaa actgggagcc gccgcatttt
     2461 tccttgcggt aatactgggt gttagtgctg gcgttattgt cgcattaaaa caaaacacca
     2521 aatgggacta taccgtgatg gggctggcaa tgaccggggt tgttatcccc agttttgtgg
     2581 ttgcgccatt attagtcatg atatttgcga tcattttgca ttggctgccg ggcggtggct
     2641 ggaatggtgg ggcgcttaaa ttcatgatat tgccgatggt ggcgttgtca ctcgcttata
     2701 tcgccagtat tgcgcgtatt acccgaggct ctatgattga agtattacac tccaacttta
     2761 ttcgtactgc ccgggcgaaa gggttaccta tgcggcggat cattttacgc cacgcattaa
     2821 aacctgctct gttacccgtg ctctcttata tcggccccgc atttgtcggc attattaccg
     2881 gttctatggt tatcgaaacc atttatggtt tgccggggat tggacaactg ttcgttaatg
     2941 gcgcattgaa ccgtgactat tccttagtgt taagcctgac catcctggtt ggtgctttaa
     3001 ccattttgtt taatgccatt gtcgatgtgc tatatgcggt tatcgacccg aaaatccgtt
     3061 actgatactg gagctcgcga tgatgttaag taagaaaaac agcgagacgc tggaaaattt
     3121 cagtgaaaag ctggaggtcg aagggcgcag cttgtggcag gacgcacgtc gacgttttat
     3181 gcataaccgt gcggcggttg ccagtctgat agtgctggtg ctgatcgcgt tatttgtaat
     3241 cctggcaccg atgctttcgc agtttgccta tgacgatact gactgggcga tgatgtccag
     3301 cgccccggat atggagtccg gtcactactt tggtactgac tcatccggtc gcgacctgct
     3361 tgtgcgcgtt gcgattggcg ggcgtatctc actcatggtc ggtgttgctg cggcactggt
     3421 ggcagtggtc gtggggacac tttacggttc gctttccggt tatctgggcg gtaaagtgga
     3481 ttcggtaatg atgcgtctgc tggaaatcct caactccttc ccattcatgt tcttcgtcat
     3541 tttgcttgtg acctttttcg gtcaaaacat cctgctgatt ttcgtggcga ttggcatggt
     3601 ttcctggctg gatatggctc gtattgtgcg tggacaaacc ctgagtctga agcgcaaaga
     3661 gtttattgag gcggcacaag ttggcggtgt atcgacatcg ggcattgtta ttcgccacat
     3721 tgtgccgaat gtactcggtg tggtggtggt ctacgcatca cttctggtgc ccagcatgat
     3781 cctctttgaa tctttcctta gcttcctggg gttgggtacg caagagccgt taagcagctg
     3841 gggtgcattg ctgagtgatg gcgcgaactc gatggaagtc tctccatggt tattgttgtt
     3901 cccagcggga ttcctcgtgg tgacgctgtt ttgtttcaac tttatcggcg atggcttgcg
     3961 tgatgctctc gacccgaaag atcgttaagg agtgcagcca tgagcgtaat tgaaactgca
     4021 actgtgccgc tcgcacaaca acaggctgac gcactgctga acgtgaaaga tttgcgtgtc
     4081 acctttagta ccccggacgg cgatgtcacg gcggtcaatg atttgaattt ttctctacgt
     4141 gccggagaaa cgctgggcat tgtaggtgag tctggttcgg gtaaatcgca aactgcattt
     4201 gcgttgatgg gcctgttggc tgccaacgga cgtattggcg gatcggcaac cttcaatggg
     4261 cgtgaaatcc tcaatttgcc agagcgtgaa ctcaataaac tgcgcgctga acaaatctca
     4321 atgatttttc aggacccaat gacttcgttg aatccctata tgcgcgtcgg tgagcagttg
     4381 atggaagtgc tgatgctgca taagaacatg agcaaagctg aggcgtttga agagtcggtg
     4441 cggatgctcg atgcggtaaa aatgccggaa gcacgtaaac gcatgaaaat gtacccgcac
     4501 gaattttctg gtggcatgcg tcagcgagtt atgattgcga tggcattgct atgtcgacct
     4561 aagctgctga ttgcggatga acccactact gcgctggacg tcaccgtaca ggcgcagatc
     4621 atgacgctat taaatgaact gaagcgggaa tttaataccg ccatcattat gattacccac
     4681 gatcttggcg tggtggcggg gatctgcgac aaagtgctgg taatgtacgc cgggcgcacg
     4741 atggaatatg gcaacgcgcg cgatgtcttt tatcaacccg ttcatcctta ttctatcggt
     4801 ttgctcaacg cggtgccgcg tctcgatgcg gaaggtgaaa caatgttgac catccctggt
     4861 aatccgccaa acctgctgcg attaccgaaa ggttgcccgt tccagccacg ttgtccgcat
     4921 gcgatggaaa tttgtagtag cgcaccgccg ctggaagagt ttacgcctgg ccgtctgcgt
     4981 gcttgcttta aaccggtgga ggaactgtta tgaatgctgt aaccgaagga agaaaagtcc
     5041 tcctcgaaat cgccgatctg aaagtgcact ttgaaatcaa agatggcaaa cagtggttct
     5101 ggcaaccgcc gaaaactctc aaagccgtcg atggtgtcac gcttcgcctg tatgaagggg
     5161 aaacattagg tgtggtaggg gaatcgggat gcggtaagtc cacctttgct cgcgccatca
     5221 ttggcctggt taaagcgacc gacggtcatg ttgcctggtt aggtaaagag ttgctgggca
     5281 tgaagcccga tgaatggcgt gccgttcgca gtgatattca gatgattttc caggatccgt
     5341 tggcatcgct aaacccgcgt atgaccatcg gcgagatcat cgctgaacca ctgcgtactt
     5401 atcatccgaa aatgtcacgc caggaagttc gcgagcgcgt gaaggcgatg atgctgaaag
     5461 tcgggttatt gcctaacctg attaaccgct atccgcatga gttctccggt gggcagtgcc
     5521 agcgtatcgg gattgctcgt gctcttattc ttgaaccgaa gctgattatc tgcgatgagc
     5581 cgatttcggc gctggacgtg tcaattcagg cgcaggtggt caacctgctc cagcagctgc
     5641 aacgtgagat gggattgtca ttaattttta tcgctcatga cctggccgtg gtaaaacaca
     5701 tttccgatcg tgtgttggtg atgtatctcg gccatgcggt agaactgggg acctatgatg
     5761 aggtctacca caatccacta catccttaca ccagggcatt gatgtcggca gtccccatac
     5821 ctgatccgga tctggagaag aacaaaacca tccagttact ggaaggggaa ttaccgtcgc
     5881 cgatcaaccc gccttccggt tgtgttttcc gtacctgttg cccgattgcc ggtccggagt
     5941 gcgccaaaac acgtcctgtg ctggaaggga gtttcagaca cgccgtttct tgcctgaaag
     6001 tcgatccgct ttaaacacaa taagggctga cagttgtcag ccctttttca cgccgaaagc
     6061 gattatttat tcccgccaga tgatatggca aagtttgtga tctttttcgc ggcataacag
     6121 aatgcgggcg aaaacatcgt tgatttcacc atcctcactg tccgccagac caatcaccac
     6181 ttcggcaaaa aagtccgggt tcaaatcgaa atcaacatgt tcctgccagt cttccgccgg
     6241 atcgaataac tccgcgccgc cgcgctcttc aaactgaaga ttgaacagca gaacatcagc
     6301 tgggtcgaga ttgtcggcag ccagttcgag aaaaatatcg taggcctgct caagcgtttc
     6361 atcttcagtc aggcgattgt ttagatccat atccatagtc actacctgtt taacctctgt
     6421 tggcgacgtt ttacagcaac gggctgaaga agtaaaacag tcgctcggcg acacgttgcc
     6481 ataatggacg ttttagccat aaacgggcat cgagcagacg tgaacgcgaa atataatcgt
     6541 cctgaacggc ggcaaggtct gcaccaaaac ctttatcgtc gattgccagg gtaatctcga
     6601 aattcagcca cagactacgc atatcaaggt taacggtgcc aaccagactt agttcgccat
     6661 cgaccagcac gctcttggta tgcagtaacc cgccttcaaa ctgataaatt ttaaccccag
     6721 cagcaagcag ttccgtaaag aatgcgcgac tggcccagcc gaccagcatc gagtcatttt
     6781 ttcgcggaag gataatactg acatccaccc cgcgctgcgc cgccgtgcaa atcgcatgaa
     6841 gtaaatcatc gcttggcaca aagtagggcg ttgtcatgat caaatattca cgcgccgaat
     6901 aagccgcagt caataatgcc tggtgaatga gatcttccgg aaagccgggg ccagaagcaa
     6961 ttgtgtgaat ggtgtgaccg ctggcctgtt caaacggcat aatattgaca tctggtggtg
     7021 gcggcagaat acgttttccg gtttcaatct cccagtcgca ggaataaata atccccatcg
     7081 cggtggcgat ggggccttcc atacgcgcca tcagatcaat ccattgccct acgcccgcat
     7141 cttgtttgaa gtagcgagga tcgaccatat tcatgctgcc ggtgtacgcg atgtaattat
     7201 cgatcatgat catcttgcga tgttggcgca ggtccatacg gcgtaaaaac acacgcatca
     7261 gattgacctt taaggcttcg accacttcaa taccggcatt acgcattagc tcgggccacg
     7321 ggctgcggaa aaaagccaca ctcccggcgg agtcgagcat caatcggcaa tgaataccac
     7381 gccgtgcagc cgccattaat gattcagcca cctggtccgc catgccgccg ggctgccaga
     7441 tataaaacac catctcaata ttatggcgcg cgagctggat gtcgcggatt aacgcctgca
     7501 tcacatcatc tgactcggtc atcagttgta gctgattccc tttgacccca gcgatcccct
     7561 gacgacgctc gcaaagcttg aataatggcg cagcgacact gctattttct tcggcgaaga
     7621 tatgcttaca ggctttaagg tcgttaagcc attttgcggt ggaaggccac atcgctctgg
     7681 cgcgctcagc gcggcgtttg cctaaatgga gctcgccaac ggcaagatag gcaataattc
     7741 cgactaacgg cagaatgtaa ataatcaaca gccaggccat cgcggaggga actgcgcgtc
     7801 gtttcattag aatgcgtaaa gttacgcctg caatgagcaa ccagtatccc agaatggcca
     7861 accaactcac caacgtataa acggttgtca tagattagaa atccttttga aagcgcattg
     7921 ttatgagttt acgcatatgg attcatctgg caaataaaaa cgcgggaaaa gcactggtca
     7981 gcgtcggggg tgggggtata atgaccattc tgttattgca tagagtagtt aacatgaagc
     8041 ggagtagaac ggaagtgggg cgctggcgca tgcagcgtca ggctagccga cgtaaatcgc
     8101 gttggcttga ggggcaatcg cgccgaaata tgcgtatcca cagcatcagg aagtgcattc
     8161 taaacaaaca gcgtaactcg ttattgtttg cgatctacaa tatctaaata tactgggcac
     8221 cattgcggtg cccaataagt ttactctttc gtctttaaac tctatttttg cactgattct
     8281 ttactgtcag ctttcgtttc ttgttcatcg ttatcgctga aaatgccatg accggagttc
     8341 aacaacattg aaacgagcat atcattatta atctcttcac catttaaaac tcgcgccaga
     8401 atttcgctgc caaacagttg cggtgagaga atgatatccg gatgtaccat cttaatctta
     8461 ttcaggtttt tgctatcact gacggcgaga actgttttga catcactgct catatctttt
     8521 gccgagagta caacaaacgc gttatctgca tcgttatcac tcagcgccag aatggcccgg
     8581 caacgatcga ttcccgcttt ctttaatact gaactgtcat tactgtcacc ggggataaca
     8641 tcagcgttat cgcctaagcg ttgctcaagt tgcttgatat catcttcagg caagttgctg
     8701 ataaccgtta cgttttgtcc gcgttgattc agttgcagga tcgtattgat ggcgagaatc
     8761 gaatgtccgc aaacaataaa atgatcttta cgatgcattg tatgattgtt tccttttaca
     8821 agtttgttga atcccccgcg gataagcgga ccaaaaattg aggtcataga tgtggcaaaa
     8881 acggtaatgc cggaaataat gaccgaaata gtgaacaatc gtgctgattc agaaacaggg
     8941 acaatatcgc catagccgac ggttgacatg gtttctatcg aaaaatagaa cgcggtcatc
     9001 aaactttcta ttcgcggatt aaaaccttcg cttaaataaa gcgcaccgta ggttgagtaa
     9061 aacagtaatg tcgtgaaact aataaatgca aaaattgtcc cggctgcggc gctactgtgg
     9121 gagaagtctt tgcgcagtat cagcaaaaac accagcgtaa aaatgcaaaa tccaatacta
     9181 aatttcagcc agggataaaa atgcagggtg taaattagcg ctatcaacaa caaaatgata
     9241 ctgattgccc atgcgagctt tgcccggaac agtaagccaa tggagttcag gacaagaaag
     9301 acgccgagca taaatagtgg ggcgtttgcc agcaaggaga gatccaactc tgaaaatgct
     9361 ttgatatgga aaatatcaag caaattgacc gacataccat agattgtttt aaaaataagc
     9421 aatccattta aaaagacggc cagcgcgaga atgtcgtgcc gaagggtcac ccataaattt
     9481 gttgctgttt gtttgaatgt agcccagtga ctcactgata cctctgtaac cacaattttg
     9541 tttaattatc ttagcgtgca cattttctga acatacatgc agcgcgtgaa tgtgttaaac
     9601 aatagtagat gactttatcc gcagtaatca aaggatttga gcgagcaact gtacctcagg
     9661 aaaggtcatt tttcctgaat atgctcacat catataaaga aatacagata aagttattat
     9721 ctgcttgtgg tagtgaatgc actgaccggc tataa
//
LOCUS       DAZPJP010000147         9739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPJP010000147 DAZPJP010000000
VERSION     DAZPJP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.147"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            39..7104
                     /gene="yeeJ"
                     /locus_tag="ACHU9J_003209"
                     /pseudo
     CDS             39..7104
                     /gene="yeeJ"
                     /locus_tag="ACHU9J_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7366..8418)
                     /locus_tag="ACHU9J_003210"
     CDS             complement(7366..8418)
                     /locus_tag="ACHU9J_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR1906678.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            8733..>9739
                     /gene="shiA"
                     /locus_tag="ACHU9J_003211"
     CDS             8733..>9739
                     /gene="shiA"
                     /locus_tag="ACHU9J_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR1906679.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         2682 a         2359 c         2341 g         2357 t
ORIGIN      
        1 agggcgcatt tatgttagct cgttcaggga aggtaagcat ggctacgaag aagagaagtg
       61 gagaagaaat aaatgaccga caaatattat gcgggatggg aattaaacta cgccgcttaa
      121 ctgcgggtat ctgtctgata actcaacttg cgttccctat ggctgcggca gcacaaggtg
      181 tggtaaacgc cgcaacccaa caaccagttc ctgcacaaat tgccattgca aatgccaata
      241 cggtgcccta cacccttgga gcgctggaat cggcccaaag cgttgccgaa cgtttcggta
      301 tttcggtggc tgagttacgc aaactcaacc agtttcgtac gtttgctcga ggttttgata
      361 atgtccgcca gggtgatgaa ctggatgtcc cggcacaagt tagtgaaaaa aaattaaccc
      421 cgccgccggg taatagcagt gacaacctcg agcaacagat agccagtact tcacagcaaa
      481 tcgggtctct gctcgccgaa gatatgaaca gcgagcaagc ggcaaatatg gcgcgtggat
      541 gggcctcttc tcaggcttca ggcgcaatga cagactggtt aagccgcttc ggtaccgcaa
      601 gaatcacgct gggcgtggat gaagatttta gcctgaagaa ctcccagttc gattttctcc
      661 atccgtggta tgaaacgcct gataatctct ttttcagtca gcatactctc catcgtactg
      721 acgagcgtac gcagattaac aacggcttag gttggcgtca tttcactccc acatggatgt
      781 cgggcatcaa cttctttttc gaccacgatc ttagccgtta ccactcccgc gccggcattg
      841 gcgcggagta ctggcgcgac tatctaaaat taagcagtaa cggctatttg cgactgacca
      901 actggcgcag cgcacctgaa ctggacaacg attataaagc acgcccggcc aatggctggg
      961 atgtacgcgc agaaagctgg ctacccgcct ggccgcacct tggcggtaaa ctggtctatg
     1021 aacagtatta tggcgatgaa gtggccctgt tcgataaaga cgatcggcaa agtaatcctc
     1081 atgccataac cgctggactt aactataccc ccttcccgct gatgaccttc agcgcggagc
     1141 aacgccaggg taaacagggc gaaaatgaca cccgttttgc cgtcgatttt acctggcaac
     1201 ctggcagcgc aatgcagaaa cagcttgacc cgaatgaagt cgctgcacgg cgtagccttg
     1261 caggcagccg ttatgatctg gtggatcgca acaacaatat cgttctggaa tatcgcaaaa
     1321 aagaactggt tcgcctaacc ctgacagacc ccgtgacagg gaagtcagga gaagtgaaat
     1381 cactggtttc gtcgctacaa accaaatatg ccctgaaagg ctataacgtc gaagccaccg
     1441 cactggaagc tgccggtggc aaagtggtca caacgggtaa agatattctg gttaccctgc
     1501 cggcttaccg gttcaccagt acgccagaaa ccgataacac ctggccgatt gaagtcaccg
     1561 ccgaagatgt caaaggcaat ttgtcgaatc gtgaacagag catggtggtc gttcaggcac
     1621 ctacgctaag ccagaaagat tcctcggtat cgttaagtac ccaaacattg aacgcggatt
     1681 cccattcaac cgccacactg acttttattg cgcatgatgc agcaggtaat cctgttgtcg
     1741 ggctggtgct ctcgacgcgt cacgaaggtg ttcaggacat caccctttct gactggaaag
     1801 ataatggtga cggaagctat acccagatcc tgaccacagg agcgatgtct ggcacgctga
     1861 cgctgatgcc acagctgaat ggtgtggatg cggctaaagc ccccgccgtg gtgaatatca
     1921 tttctgtttc gtcatcccga actcactcgt caattaagat tgataaggac cgttatctct
     1981 ccggcaatcc tatcgaggtg acggtagaac tgagagatga aaatgacaaa cctgttaagg
     2041 aacaaaaaca gcaactgaat aacgcagtca gcatcgacaa cgtgaaacca ggagtcacta
     2101 cagactggaa agaaaccgca gatggcgtct ataaggcgac ctataccgcc tataccaaag
     2161 gcagtggact tactgcgaag ctattggaat gaagatttgc ataccgctgg atttatcatc
     2221 gacgccaacc cgcagtcagc gaaaattgcg acattatctg ccagcaataa tggtgtactc
     2281 gccaatgaga atgcagcaaa caccgtctcg gtcaatgtcg ctgatgaagg aagcaaccca
     2341 atcaatgatc ataccgtcac gtttgcggta ttaagcggat cggcaacttc cttcaacaat
     2401 caaaacaccg caaaaacgga tgttaatggt ctggcgactt ttgatctgaa aagtagtaag
     2461 caggaagaca acacggttga agtcaccctt gaaaatggcg tgaaacaaac gttaatcgtc
     2521 agttttgtcg gcgactcgag taccgcgcag gttgatctgc agaagtcgaa aaatgaagtg
     2581 gttgctgacg gcaatgacag cgccacaatg accgcgaccg tccgggatgc aaaaggcaac
     2641 ctgctcaatg acgtcatggt cactttcaat gttaattcag cagaggcgaa actgagccaa
     2701 accgaagtga atagccacga cgggatcgcc acagctacgc tgaccagttt gaaaaatggt
     2761 gattataggg ttacggcctc tgtgagctct ggttcccagg ctaatcaaca ggtgaatttt
     2821 atcggtgatc aaagtactgc tgccctgacc ctcagtgtgc cttcaggtga tatcaccgtc
     2881 accaacacag ctccgcaaca tatgactgca accttgcagg ataaaaatgg caacccacta
     2941 aaagataaag aaatcacctt ctctgtgcca aacgacgtcg caagtaagtt ctcgattagc
     3001 aacggaggaa aaggcatgac ggatagtaac ggggttgcaa tcgcctccct gaccggcacg
     3061 ttagcgggca cgcatatgat catggctcgt ctggctaaca gcaatgtcag cgatgcacag
     3121 ccaatgacgt ttgtggcgga taaagacaga gcggttgtcg ttttgcaaac atcgaaagcg
     3181 gaaatcattg ggaatggcgt ggatgagaca actctgacag caacagtgaa agatccgtcg
     3241 aatcatccgg tggcggggat aacggtaaac ttcaccatgc cacaggacgt tgcggcaaac
     3301 tttacccttg aaaataacgg tattgccatc actcaggcca atggggaagc gcatgtcacg
     3361 ctgaaaggta aaaaagcggg cacgcatacg gttaccgcaa cgctgggtaa taacaatacc
     3421 agtgattcgc agccggtaac atttgtggcg gacaaagcct cggctcaggt tgtcctgcag
     3481 atatcaaaag atgagatcac aggtaatggc gtcgatagcg caacgctaac tgcaacggtt
     3541 aaagatcagt tcgacaatga ggtgaataat cttccggtaa cattcagctc agcctcttca
     3601 ggactcaccc tgaccccggg agtaagtaat accaatgagt ctggcatcgc gcaggccact
     3661 ctcgcaggcg ttgcctttgg tgagcagacg gtcactgcat cactggctaa taatggtgcc
     3721 agcgacaaca aaactgtgca ttttattggc gacacagcgg cggcaaaaat tatcgagttg
     3781 acgcctgtcc cagacagcat aatcgccggt accccgcaga acagctccgg cagcgccatc
     3841 accgccacag tcgttgataa taatggcttt ccggtgaaag gtgtgactgt gaacttcacc
     3901 agcagaacaa actctgccga aatgacgaat ggcggccaag ccgtaacgaa cgaacagggt
     3961 aaggctaccg tcacttatac caatacccgc tcctcgatag aatcaggagc gagaccggat
     4021 accgttgagg ccagtctgga aaatggtagc tccacgctta gcacatcaat taatgtcaac
     4081 gctgatgcgt ctacggcaca tctcacctgg ctacaggcac tttttgatac agtctccgca
     4141 ggcgagacaa ccagtctgta tattgaggtg aaggataatt acggcaacgg tgtcccccag
     4201 catcaggtga ccctcagcgt ttcaccaagt gaaggcgtga cccccagtaa taacgctata
     4261 tatactacca accacgacgg caatttttac gcaagcttta ccgctacaaa agccggggtt
     4321 tatcaagtga cggcaaccct cgaaaatggc gattcgatgc aacaaacagt gacctatgtg
     4381 ccgaacgtca cgaatgccga aatcacgctg gcaacctcga aggatccggt gattgccgac
     4441 aataacgatc tcacgacact aacagcaaca gtcgctgata cagagggcaa tgcgatagcc
     4501 aacactgaag taacatttac tctgccggaa gatgttaagg cgaacttcac gctgagcgat
     4561 ggcggtaaag cgattactga tgctgaaggc aaagcgaaag tcacgctgaa aggtacaaaa
     4621 gcaggcgctc atactgttac agcatcgata actggcggta agagtgagca gttggtggtg
     4681 aactttactg cggatacgct cactgcgcag gttaatctta acgttaccga ggacaatttt
     4741 atcgctaata acgtcgggat gaccaggctg caggcaacag tgactgatgg aaacggcaac
     4801 ccgttagcca atgaggcggt gacattcacg ctaccggcag atgtgagcgc aagctttact
     4861 ctcggacaag gcggttccgc cattactgac atcaacggca aggctgaagt tacactgagc
     4921 ggtacaaaat ccggcaccta ccccgtgaca gttagcgtga acaattatgg tgtcagtgat
     4981 acgaaacagg tgactttgat tgccgatgct ggtaccgcaa cactagcctc cttaacctct
     5041 gtatactcat tcgtcgtcag cacgaccgag ggcgcgacca tgactgcaag cgtcactgac
     5101 gctaacggca acccggtaga aggcataaaa gttaatttcc gcggaacctc cgtcacgcta
     5161 agcagcacca gcgttgaaac ggatgatcgg ggtttcactg aaattcttgt gacaagcacc
     5221 gaagtcggac tgaaaacagt ttcagcctct ctggcagata aacctactga agtcatatcg
     5281 cgattactga atgcaaaagc agatattaat tctgcaacga ttaccagtct ggagatacct
     5341 gaaggtcagg tcatggtcgc acaagacgta gcagttaaag ctcacgtcaa cgaccagttt
     5401 ggcaacccga ttcttaatga atctgtaaca ttcagtgcag agccaccaga gcacatgacc
     5461 atcagccaaa atattgtctc tactgatacg catggtatag ccgaggtctc catgacgccc
     5521 gaaagaaacg gttcgtatat ggtgaaagca tccctggcga atggagcctc acttgagaaa
     5581 caactggagg ctattgatga aaaactgaca ctctcggcgt ccagcccgct tatcggtgtc
     5641 aattccccaa caggtgcaac tctgacggca acgctaactt ctgcaaatgg cactccagtg
     5701 gagggtcagg tcatcaactt tagcgtaacg ccagaaggtg cgacgttaag tggcggaaaa
     5761 gtgagaacta actcttctgg tcaggctcca gtcgttctga ccagcaataa agtcggtaca
     5821 tatacggtga ctgcatcgtt ccataacggc gtaacaatac agacacagac aaccgtgaaa
     5881 gtcactggca actcaagcac cgcccatgtt actagcttta tcgctgatcc atcgactatc
     5941 gccgccacca acagtgattt aagtacctta aaggcaacgg ttgaggatgg cagtggtaac
     6001 ctgatcgaag gtctcactgt gtactttgcc ttaaaaagcg gctctgccac attaacgtca
     6061 ttaacagcgg tgaccgatca aaacggaatc gcgacaacaa gcgtgaaagg agcgatgaca
     6121 ggtagcgtca cggtaagcgc agtcacgacc gctggtggaa tgcaaacagt agatataacg
     6181 ctggtggcag gcccagcaga cgcctcaaag tcagtcctta agaacaatcg gtcatcattg
     6241 aaaggagact ttaccgatag tgctgagcta catcttgttc tgcacgatat atcaggcaat
     6301 ccgatcaaag tttctgaagg gctggaattt gtacagtcag gtaccaacgt gccctatgtg
     6361 caagttagtg caattgacta cagtaaaaat ttctcaggcg agtacaaagc cactgttaca
     6421 ggaggcggag agggtatcac aacgctgatc cctgtattga atggtgttca tcaagctggt
     6481 ctgagtacca caatacaatt cactcgcgca gaagacaaaa taatgagcgg tacagtatca
     6541 gtcaatggta ctgacctacc gacaactaca ttcccttcgc aggggttcac cggggcgtat
     6601 tatcagttga ataatgacaa ctttgcccca ggaaaaacgg cggctgatta tgagttttca
     6661 agctctgcct cctgggtcga tgttgatgct accggtaaag tgacatttaa aaatgtcggc
     6721 agcaattcgg aaaggattac ggcgacacca aaatcaggag gccctagcta tgtatacgaa
     6781 atccgtgtga agagttggtg ggtgaacgcc ggcgaggctt tcatgatata cagccttgct
     6841 gaaaattttt gcagcagcaa tggctacacg ctccccagag caaactattt aaaccacagt
     6901 agttcccgag gcatcgggtc actgtacagt gaatggggag atatggggca ttacacgact
     6961 gaagctggtt ttcaatcaaa tatgtattgg tcatctagtc ccgcaaactc aagcgaacaa
     7021 tacgtagttt ccctggcaac aggtgatcaa agcgtatttg aaaagcttgg gtttgcttat
     7081 gcgacatgtt ataaaaacct ctgattttcc gttataattt cttaaagagt gctccattcg
     7141 gagctctctt ttttacattc cctctataga gcttgccctt ctctcacaga aaatgaataa
     7201 ataaaaatgc ggcaccgcca gaattgcgtt cgatgtgact atgatcgtat ccggtgagta
     7261 ctgctttgat tcaaaggctg gttttctgtg atgattgacc agcctcagag tattgatgac
     7321 accaccgttc ataacagatc ggcattacta tgcataagta ctgtctcata ttctcgccaa
     7381 aacacttttt aatgcctttg cgaattcatc cagacttata ttgtcaattt tttgcccaac
     7441 gacgactgac tccgagacat cggccattgc gggccatcgc gttttgtaat cagcataaat
     7501 tgcaacaaca ggggtattat aggcacttgc catatgtaat attgaggtgt caggagttat
     7561 tgcgatataa gcatctttta tgattgctgc tgaacgttta atcgagggtg gtaaggataa
     7621 acggtagaca ttattataac aatccactaa tgccctggct ttatcttcac ctttgggtcc
     7681 atgaacgatc actattggta tatctgtttc actttgaatt tttgctatta gattttctgc
     7741 aatcgataat gaaaatgtac gttcctgcga gctaccttct aaattgaacg caatgtaaga
     7801 tcctaacgat cgcatttcct cgcgcacctc atcgagtaca tcctcactta gtggcaattc
     7861 atatattggc ctgactgctg gaaaacccgc ctcacgcatc ataaatgccc atgtcatagg
     7921 tacgggagcc cggagtttct gatccatacg ggacgcgttc ttgcacaaag gggagtagca
     7981 attcatggtt ataccaacaa cctgaaaatt cgtttttgct ttcaactgac tgataaataa
     8041 catcgttttc aggttctttt tacgcatccc ctcaatacaa agatccggcg taccgtattg
     8101 ctgtgttatg ttctttgcta aatcttttat ttcttttaat gttgcgtgat cctgcatagt
     8161 cattgtgact aatgttaatt tagtctgttc aagtttaagc gcattaaaga cttctaaatt
     8221 aattgtcgac gttacaatta aaagatgctt aattttatgc aattcaagcg cccgaataac
     8281 aggaaagatg gccatagcat cgccaatctg atcgggaata tggatgacaa caaagtctgt
     8341 tttttcaata ttgaaattat aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa
     8401 caatgatgct aaaaacatac ctaacctcgc ctccctactg gttataatgc aatgcagtct
     8461 atcagactca tcagggtgcc attttgtgca tatgcggact tttatgtttc atatctctaa
     8521 cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc
     8581 atcgaatttt tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg
     8641 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
     8701 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc
     8761 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agcttcgctg
     8821 gtgccgtcgt cgactggtat gattttttac tctatggcat caccgccgca ctggtgttta
     8881 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgctacct
     8941 ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc
     9001 gactgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct
     9061 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga
     9121 cactgcgtgt cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt
     9181 ccgttgaaag tgcaccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct
     9241 acggtgtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg
     9301 acgaacagtt tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggtac
     9361 tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac
     9421 attatcaagc tgccgcgaaa aaacgcatcc cggttatcga agcgctgtta cgacatcccg
     9481 gtgctttcct gaagattatt gcgctacgac tgtgcgaatt gctgacgatg tacatcgtta
     9541 ctgcctttgc acttaattat tcaacccaga atatggggct accgcgcgaa cttttcctta
     9601 atattggttt gctggtaggt ggattaagct gcctgacaat tccctgtttt gcctggcttg
     9661 ccgatcgttt tggtcgccgt agggtttata tcacaggtgc gttaatcgga acgttgagcg
     9721 catttccttt ctttatggc
//
LOCUS       DAZPJP010000148         9737 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPJP010000148 DAZPJP010000000
VERSION     DAZPJP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.148"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..56)
                     /locus_tag="ACHU9J_003212"
     CDS             complement(<1..56)
                     /locus_tag="ACHU9J_003212"
                     /inference="COORDINATES: protein motif:HMM:NF015750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1906680.1"
                     /translation="MASVSISCPSCSATDGVVR"
     gene            114..2315
                     /locus_tag="ACHU9J_003213"
                     /pseudo
     CDS             114..2315
                     /locus_tag="ACHU9J_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2308..3444
                     /locus_tag="ACHU9J_003214"
     CDS             2308..3444
                     /locus_tag="ACHU9J_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1906681.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3441..5441
                     /locus_tag="ACHU9J_003215"
     CDS             3441..5441
                     /locus_tag="ACHU9J_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1906682.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5566..6027
                     /locus_tag="ACHU9J_003216"
     CDS             5566..6027
                     /locus_tag="ACHU9J_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1906683.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(6068..6538)
                     /locus_tag="ACHU9J_003217"
     CDS             complement(6068..6538)
                     /locus_tag="ACHU9J_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1906684.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6585..7304)
                     /gene="btsR"
                     /locus_tag="ACHU9J_003218"
                     /gene_synonym="yehT"
     CDS             complement(6585..7304)
                     /gene="btsR"
                     /locus_tag="ACHU9J_003218"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1906685.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7301..8986)
                     /gene="btsS"
                     /locus_tag="ACHU9J_003219"
     CDS             complement(7301..8986)
                     /gene="btsS"
                     /locus_tag="ACHU9J_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1906686.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9208..9273
                     /locus_tag="ACHU9J_003220"
                     /pseudo
     CDS             9208..9273
                     /locus_tag="ACHU9J_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9501..>9737
                     /locus_tag="ACHU9J_003221"
     CDS             9501..>9737
                     /locus_tag="ACHU9J_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1906687.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2395 a         2566 c         2493 g         2283 t
ORIGIN      
        1 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
       61 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac cgccagcggc
      121 cacgatacgt attgattgaa ggcccggctg attttaatga ccgggtagac gaactgtttt
      181 tagcccacca gcttccggta gctatttaca gttattgcca gtatcaggac ggtgcagccc
      241 ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc ggagtggcag gcgctacaag
      301 ccgcacgtcg tattcaggca caaacttact tcatcgattt gccttgctgg gcgcagagtg
      361 aagaagtgga cgattcgcct gatacgcaag aggaaagcca ggccttgctg ctgcgtgcca
      421 cccgcatgga taacagcgac accctgtggg atcacttgtt cgaagatgaa agccagcaaa
      481 ctgcattacc ctctgcactg gcgcactatt ttgcccaact gcggggcgat tcccccggcg
      541 atgcactcaa tcgtcagcgc gaagccttta tggcccgctg gattggatgg gcgatgcagc
      601 aaaacaatgg cgacgtgtta gtcgtctgcg gtggctggca cgctcctgca ctggcaaaaa
      661 tgtgacgcga atgcccgcag gacattaaca cgccggaatt gccctcgctg gcagatgccg
      721 ttacaggttg ttatctcacc ccctacagtg aaaagcgcct tgatgtgctg gcaggatacc
      781 tttccggtat gcctgccccg gtctggcaaa gctggtactg gcagtggggc ttacagcagg
      841 cgggagaaca actgctaaaa acggttctta cccgtttgcg ccagcacaaa ttgcctgctt
      901 cgaccgcgga tatggctgcc gctcatctgc atgcgatggc gctggcacag ttgcgcggac
      961 ataaactacc gttacgcact gactggctgg atgccatagc aggctcgctg attaaagaag
     1021 ccctgaacgc gccgttgccg tggagctatc gcggagttat tcatcccgac accgatccga
     1081 ttctgctaac gttgatagac acattagcgg gtgacggatt cggtaaactt gccccttcta
     1141 cgccacaacc gcctctgcca aaagatgtca cctgcgaact ggaacgtacc gcaatctctc
     1201 ttccggcgga gcttacctta aatcgcttta accccaatgg gctagcgcaa agtcaggtgt
     1261 tacatcggct ggcaatactg gagatcccag ggattgtacg ccagcaggga agcacactga
     1321 cacttgcagg caacggtgaa gaacgctgga aattaacccg cccgcttagc cagcatgcgg
     1381 cattgattga ggccgcctgc tttggtgcca cactccagga agccgcacgc cataaattag
     1441 aagccgatat gctggacgcg ggtggaatcg gcagtatcac cacatgtctt agccaggcgg
     1501 cgttagcggg tctggcgtcc ttcagtcaac aattactgga gcaactcaca ttattaatcg
     1561 cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga agtgctatat gccttatggc
     1621 ggctggatga aattagcggt atgcaaggcg cgcagatatt acaaacgacg ttatgcgcgg
     1681 ctatcgatcg cacgctgtgg ctgtgtgaat ctaacggcag accagatgaa aaggagtttc
     1741 acgctcacct gcatagctgg caggcgcttt gccatatcct gcgcgatcta catagcggcg
     1801 ttaatttacc cggcgttttt ctttctgcgg cggtagcctt actggagcgc cgcagtcagg
     1861 cgattcatgt cccggcgctg gatcgcggcg cggctcttgg cgcactgatg cgtctggaac
     1921 atcccaacgc cagtgccgaa gcggcgctga cgatgctggc gcagttatcc ccggcacaat
     1981 ccggcgaggc gctgcacggt ttgctggcgc tcgcccgtca tcaactggcc tgccaaccag
     2041 catttatcgc cggtttcagt agtcatttaa atcaactgag tgacgacgat tttattaacg
     2101 ccctgcccga tttgcgcgcg gcgatggcct ggctaccgcc acgagaacgc gggacactgg
     2161 cgcatcaggt gcttgagcat taccagcttg cgcagcttcc tgtttcagcg ctgcaaatgc
     2221 cgttgcattg tcctccgcaa gccattgcac atcatcaaca actcgaacag caggcactgg
     2281 catcgctgca aaactgggga gttttccatg tctgaactga acgatcttct gaccacccgt
     2341 gagctacaac gctggcgatt aattcttggc gaagcggcag aaacgacgct ttgtgggctg
     2401 gatgacaacg cccggcagat agaccacgcg ctggaatggc tgtatgggcg cgatcctgaa
     2461 cggctccagc gtggtgaacg ctccggtgga ttaggtggct caaatctcac cactcctgag
     2521 tggatcaaca gtattcacac gctgtttccg caacaggtga ttgagcggct ggaaagcgat
     2581 gccgtactgc gctacggcat tgaagatgtg gtgacgaatc tcgacatgct ggaacgtatg
     2641 cagccttctg aaagcctgct acgcgccgtt ttgcacacca aacatctgat gaatcccgaa
     2701 gtactggctg ccgcccgccg gatagtgcgc caggttgttg aagaaattat ggctcgactg
     2761 gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc gccgtcgccg ctcatttatt
     2821 ccactggcgc gaaactttga tttcaaaagt actctgcgcg ctaacctgca acactggcac
     2881 ccgcaacacg gcaagttgta tatcgaatcc ccccgcttta acagccgcat taaacgccaa
     2941 agcgaacaat ggcaactggt cttactggtt gatcaaagcg gatcgatggt ggactcggtg
     3001 atccactctg cggtgatggc ggcctgtttg tggcagttac ccggcattcg tacccatctg
     3061 gtggcgtttg acacaagcgt ggttgatctc acggcagacg ttgccgatcc ggtagagtta
     3121 ttaatgaaag tacagttggg cggcgggacc aatatcgcca gtgccgtgga gtatggtcgg
     3181 caacttattg aacaaccagc aaaaagcgtc attatcctcg tgagcgattt ttacgaaggg
     3241 ggttcatcat cattgctgac gcatcaggtg aaaaagtgtg tccagagcgg catcaaagtg
     3301 ctgggactgg ctgcgctcga tagcaccgca acaccttgct atgaccgcga tacggcccag
     3361 gcgctggtta atgtcggcgc acaaatagcc gccatgacgc cgggcgagct ggcatcatgg
     3421 cttgcggaga atcttcagtc atgaattcac tacgtccgga attattagaa ctgacaccgc
     3481 aggccctgac ggcgttaagc aatgccggtt ttgttaagcg cagtcttaag gaactggaaa
     3541 atggcaacgt cccggagatc agccatgaga acgacgcttt aatcgccacc ttcagtgacg
     3601 gtgtccgtac ccagctggcg aacggccagg cactgaaaga ggctcagtgc agttgcgggg
     3661 ccaacggtat gtgccgtcat cgcgtgatgc tggtgttaag ttatcaacga ctttgtgcca
     3721 ccactcagtc tacggaaaaa gaagaagagt gggatccggc aatctggctg gaagaactgg
     3781 ctacccttcc cgatgctacc cgcaaacgcg cacaggcgct ggtcgctaaa ggcatcacca
     3841 ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg cttaccgatg agcgatatgc
     3901 gtttttattc ccgcagcagt attcgtttcg cccgttgtga ttgtattgaa agcacacttt
     3961 gcgaacatgt cgtactggca gtacaggcct ttgtccaggc aaaagcgcag caagcggaat
     4021 tgactcattt aatctggcag atgcgcagcg aacacgtcac atcatctgac gatccgtttg
     4081 ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca actaagccag gcattatggc
     4141 tgagcggcat cagccagccg cttattcact acgaggccgc tttcagccgc gcgcagcaag
     4201 cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct acggcaactg cgcgccagcg
     4261 ttgatgcctt ccacgcccgc gccagccact atcatgccgg agaatgctta cgtcagcttg
     4321 cggcattaaa cagtcgatta aattgcgcac aagagatggc ccggcgcgac agtattggtg
     4381 aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga gaagcaaagc
     4441 ttgatcatct gcggctggtg tctttaggta tgcgttgctg gcaggatatt gagcattatg
     4501 gtttacgcat ctggtttacc gatcccgaca ccggcagtat tttgcacctt tcgcgcagtt
     4561 ggccgcgaag tgaacaggaa aactcaccgg cagctacgcg tcggctgttt agttttcagg
     4621 ctggcgcact agcgggcggg caaattgttt cacaagcagc aaaacgcagt gccgatggcg
     4681 agctgctgtt agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg cctgatgcct
     4741 ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg gaatatttac
     4801 gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt cgataactta tttattctgc
     4861 cggtcgctga gtgtatttcg ctcggttggg atagcagccg ccagacgctg gatgcgcagg
     4921 taatcagcgg cgaaggtgaa gataatctcc tgacgttatc ccttccggca tctgcctgtt
     4981 ctccttttgc cgttgaacgc atggcggcgc ttttgcaaca aacagacgac cccgtgagtc
     5041 tggtttctgg ctttgtcagc tttgtagagg ggcaattgac actggaacca cgggtgatga
     5101 tgacaaaaac ccgcgcctgg gcgctggacg cagaaactgc gcctgtggca ccgctacctt
     5161 ctgccagcgt tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc tgccaggcgt
     5221 tacttattca actgctccat aacggctggc gctatcagga acagagcgct attagtcagg
     5281 cagagttgct ggcgaatgac ctctccgcgg tcggttttta tcggctggca catgtgttgg
     5341 gacaatttcg taatacagaa agcgaggcac gggtagaagc aatgaataac ggtgttttac
     5401 tttgcgaaca attattcccc atgcttcagc aacaaggatg aaatagtgct ttttactaag
     5461 agttctactc cagttccgga ctgctcacgc cacggtatta ggcatatcct atatagcccc
     5521 tggtgagagt caccagttcc ttgattaaat aaaatggagt tttacatgaa ggctttcaat
     5581 aagctgtttt ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac
     5641 aaagaagaat cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat
     5701 gtctacaaag gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc
     5761 attggtgcaa ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac
     5821 aaaaacatcg cgggtgttga agaaaaatta acctataccg atacctacgc gcaggaaaac
     5881 gtgactatcg atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac
     5941 gtttctgctg aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa
     6001 gccgctggtt ttaaagaagt gaaataatct gtcggcggcc atgtttcgca tggccgccat
     6061 acccgtctta gcttttcttc acatgctggc gcgctgccag tccacgcaga aaataacgta
     6121 aaaattgatc gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa
     6181 tttccggcat cgaaacgcgg aactgctgtt cggtgaggat cgccagaatg tcatcggttt
     6241 tcagcgaaaa cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg
     6301 gctccagtgc cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga
     6361 atgacgacaa aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc
     6421 agacggcgat ctgttccgcg gtggcttcga cattacccag cgccagaata cgcaccaggt
     6481 cattattatt ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttagcatga
     6541 ggccttcagg tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc
     6601 tcttttaagc ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc
     6661 aactcggcct ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga
     6721 tactgacgat ggcagcgcag tagtggtgta cgactttcca gggtacgtaa tgtcaattcg
     6781 gtaaagccct ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca
     6841 aatgccacat ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat
     6901 ttcagcgcct gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc
     6961 aatcgcgcca gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag
     7021 gcatgttctt caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac
     7081 gggcgatgtt ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg
     7141 atatcgagaa acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg
     7201 tttgaacact ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac acgcaggttc
     7261 tcccgtgcta acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg
     7321 gtagtcgtaa cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt
     7381 catcgccaaa ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat
     7441 tggttaccgg ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct
     7501 cacgtcgggc gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa
     7561 tggcgttttc cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt
     7621 gcggaatagc aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat
     7681 aagcgttcac atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt
     7741 ttttgcggaa aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc
     7801 ggatcaccgc tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt
     7861 gaagcagttt gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa
     7921 gaatctgcgc cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga
     7981 ataaacggtt tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac
     8041 gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag
     8101 gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag
     8161 gcgaagaaat cggtttgcca ggtaaatggt ggtcgtcacc aattccggta aaggccagca
     8221 atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac agcacctgag
     8281 ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg
     8341 ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat
     8401 cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag
     8461 caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgcc aggatgatca
     8521 gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt
     8581 tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa
     8641 cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc
     8701 cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg
     8761 cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca
     8821 tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga
     8881 cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa
     8941 cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca
     9001 cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag
     9061 aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct
     9121 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
     9181 cgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
     9241 ctttgtgata ttaaccctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
     9301 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
     9361 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
     9421 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
     9481 cacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
     9541 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
     9601 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
     9661 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
     9721 agtctgctga cgagtgg
//
LOCUS       DAZPJP010000149         9674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPJP010000149 DAZPJP010000000
VERSION     DAZPJP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.149"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHU9J_003222"
     CDS             <1..1710
                     /locus_tag="ACHU9J_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR1906688.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHU9J_003223"
     CDS             1711..3360
                     /locus_tag="ACHU9J_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1906689.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVTGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHU9J_003224"
     CDS             3365..4141
                     /locus_tag="ACHU9J_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1906690.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHU9J_003225"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHU9J_003225"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR1906691.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHU9J_003226"
     CDS             complement(5430..6752)
                     /locus_tag="ACHU9J_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR1906692.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHU9J_003227"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHU9J_003227"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR1906693.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHU9J_003228"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHU9J_003228"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR1906694.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHU9J_003229"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHU9J_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR1906695.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2355 a         2484 c         2605 g         2230 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtcacaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZPJP010000150         9662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPJP010000150 DAZPJP010000000
VERSION     DAZPJP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.151"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..164)
                     /locus_tag="ACHU9J_003230"
     CDS             complement(<1..164)
                     /locus_tag="ACHU9J_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1906696.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(383..1360)
                     /gene="epmA"
                     /locus_tag="ACHU9J_003231"
     CDS             complement(383..1360)
                     /gene="epmA"
                     /locus_tag="ACHU9J_003231"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1906697.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1685..3493
                     /gene="frdA"
                     /locus_tag="ACHU9J_003232"
     CDS             1685..3493
                     /gene="frdA"
                     /locus_tag="ACHU9J_003232"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1906698.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3486..4220
                     /gene="frdB"
                     /locus_tag="ACHU9J_003233"
     CDS             3486..4220
                     /gene="frdB"
                     /locus_tag="ACHU9J_003233"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1906699.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4231..4626
                     /gene="frdC"
                     /locus_tag="ACHU9J_003234"
     CDS             4231..4626
                     /gene="frdC"
                     /locus_tag="ACHU9J_003234"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1906700.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4637..4996
                     /gene="frdD"
                     /locus_tag="ACHU9J_003235"
     CDS             4637..4996
                     /gene="frdD"
                     /locus_tag="ACHU9J_003235"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1906701.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5059..6192
                     /locus_tag="ACHU9J_003236"
     CDS             5059..6192
                     /locus_tag="ACHU9J_003236"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1906702.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6281..6814
                     /gene="blc"
                     /locus_tag="ACHU9J_003237"
     CDS             6281..6814
                     /gene="blc"
                     /locus_tag="ACHU9J_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1906703.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="ACHU9J_003238"
     CDS             complement(6811..7128)
                     /gene="sugE"
                     /locus_tag="ACHU9J_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1906704.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="ACHU9J_003239"
     CDS             complement(7303..7449)
                     /gene="ecnB"
                     /locus_tag="ACHU9J_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1906705.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="ACHU9J_003240"
     CDS             complement(7560..7685)
                     /gene="ecnA"
                     /locus_tag="ACHU9J_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1906706.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7737..8303)
                     /gene="efp"
                     /locus_tag="ACHU9J_003241"
     CDS             complement(7737..8303)
                     /gene="efp"
                     /locus_tag="ACHU9J_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1906707.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8345..9373
                     /gene="epmB"
                     /locus_tag="ACHU9J_003242"
     CDS             8345..9373
                     /gene="epmB"
                     /locus_tag="ACHU9J_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1906708.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2452 a         2473 c         2472 g         2265 t
ORIGIN      
        1 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
       61 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
      121 aatatgagtc ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt
      181 tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt
      241 aaaaaccaat acagaattga tacccggatg atatcaaata tccagtctta aattgtggcc
      301 tttaacggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
      361 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
      421 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
      481 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
      541 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
      601 ccgttaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
      661 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
      721 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
      781 attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac gttactcaaa tccagtttcg
      841 ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca
      901 agaaagcttg ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga
      961 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     1021 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     1081 ggaataccgg cccacaaccg gcaaccagca ggcgtttcat atggtattcc gggctggtca
     1141 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     1201 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     1261 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcacgtttt aataagttag
     1321 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     1381 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa
     1441 cagcggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     1501 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     1561 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     1621 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     1681 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     1741 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     1801 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     1861 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     1921 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     1981 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     2041 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     2101 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     2161 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
     2221 cacgctggtg cagatccgtg ctaacgcggt cgttatggct actggcggtg cgggtcgcgt
     2281 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
     2341 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg
     2401 ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa
     2461 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
     2521 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
     2581 gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca
     2641 cctcggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
     2701 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
     2761 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
     2821 tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga
     2881 actggtggtc ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
     2941 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
     3001 tctggttaac caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc
     3061 aatggaagaa ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
     3121 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt
     3181 caacaccgac ctgctctaca ccattgaact gggccacggt ctgaacgttg ctgaatgtat
     3241 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg
     3301 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gtgatgctga
     3361 tggcacgact cgcctagagt acagcgacgt gaagattact acgctgccgc cagctaaacg
     3421 cgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
     3481 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
     3541 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
     3601 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc
     3661 gtatggcgat ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
     3721 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
     3781 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
     3841 cgtacatcat cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc
     3901 agatggcaaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
     3961 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
     4021 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
     4081 agaacggcgt atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg
     4141 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
     4201 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
     4261 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
     4321 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
     4381 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
     4441 atcattaacc tgatcactct ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg
     4501 gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
     4561 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
     4621 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
     4681 gggcctcttc ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct
     4741 ggtgggtatt ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt
     4801 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
     4861 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc
     4921 tgcgggcaaa tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg
     4981 tatcgttaca atctaacgta tcgccaatgt aaatccggcc cgcctatggc gggccgtttt
     5041 gtatggaaac cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct
     5101 tgctccacat ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
     5161 cttatagagc aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct
     5221 tattacttta cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg
     5281 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
     5341 gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttactgct
     5401 aaacagtgga atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca
     5461 ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
     5521 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
     5581 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc
     5641 ttccagccac tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat
     5701 tacgcctggg gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct
     5761 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
     5821 accccccttg atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc
     5881 tactggcaaa ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc
     5941 aatcctgaca gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta
     6001 aaagcgatta agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
     6061 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
     6121 ctggctaaca aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac
     6181 gctctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc
     6241 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
     6301 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg
     6361 cgtgacagta gtgaataatt tcgacgctaa acgctatctc ggtacctggt atgagattgc
     6421 ccgttttgat caccgttttg aacgtggact ggaaaaagtc accgcaacat acagtctgcg
     6481 tgatgacggc ggcctgaatg tcatcaataa aggctataac cctgacagag aaatgtggca
     6541 gcagagtgaa gggaaagcgt actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc
     6601 attctttggt cctttctatg gcggttataa cgtcattgca cttgatcggg aataccgcca
     6661 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
     6721 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
     6781 taagtttatt tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat
     6841 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag
     6901 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
     6961 ccctaccggt aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt
     7021 cacagtaata acacttggcg tcaaacgact aaagccgtgg gtatatttca ggccaacggc
     7081 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
     7141 agaattgggg ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg
     7201 ttgaacggat attttgccag gtggcagggg aatttcaact attacctttg tgactatcgc
     7261 aaaaaaacaa acggcacgac ataactgtcg taccgttatt gcttattgct gcgctttcgt
     7321 tgctgcacca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     7381 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     7441 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     7501 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat
     7561 tagctggcag cgcgggagat ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga
     7621 gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc
     7681 atcatctctg acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     7741 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt
     7801 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     7861 cctgcggtat cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     7921 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     7981 agcagccatt tagcgttgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt
     8041 tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg
     8101 acaacatcag cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg
     8161 gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat
     8221 tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa
     8281 tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca
     8341 caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact
     8401 tgccgatgtt gtgaccgatc ctggtgaact tctgcgtctt ttgaatatag acgcggacga
     8461 aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt
     8521 tatcgatcgc atggagaaag gcaatccgga cgatcctatt ttgcgtcagg tacttacctc
     8581 gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag
     8641 cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg
     8701 ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg
     8761 caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga
     8821 gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct
     8881 cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat
     8941 tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca
     9001 aatcttgctg gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc
     9061 gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg
     9121 tgtgaacgat aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt
     9181 gatgccgtat tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag
     9241 tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt
     9301 gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct
     9361 acgccagcag taactgatta aacagggaag ttaaacatta gcctgatgcg ctacgcttat
     9421 caggcctaca ttgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg
     9481 cgtttacgcc gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg
     9541 ggaaatttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttgtta
     9601 ctctttatat agccaacacc aataattaac ctttcttttc tgtttatata gagtgagtac
     9661 gg
//
LOCUS       DAZPJP010000151         9648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPJP010000151 DAZPJP010000000
VERSION     DAZPJP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.152"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHU9J_003243"
     CDS             complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHU9J_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1906709.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHU9J_003244"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHU9J_003244"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1906710.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="ACHU9J_003245"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="ACHU9J_003245"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1906711.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="ACHU9J_003246"
     CDS             complement(5701..7017)
                     /locus_tag="ACHU9J_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1906712.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="ACHU9J_003247"
     CDS             complement(7007..8764)
                     /locus_tag="ACHU9J_003247"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1906713.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9648)
                     /locus_tag="ACHU9J_003248"
     CDS             complement(8780..>9648)
                     /locus_tag="ACHU9J_003248"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1906714.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcga
//
LOCUS       DAZPJP010000152         9501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPJP010000152 DAZPJP010000000
VERSION     DAZPJP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.153"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHU9J_003249"
     CDS             <1..108
                     /locus_tag="ACHU9J_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1906715.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHU9J_003250"
     CDS             complement(149..1231)
                     /locus_tag="ACHU9J_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR1906716.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHU9J_003251"
     CDS             complement(1228..3060)
                     /locus_tag="ACHU9J_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1906717.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHU9J_003252"
     CDS             complement(3275..3631)
                     /locus_tag="ACHU9J_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906718.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHU9J_003253"
     CDS             complement(3689..4111)
                     /locus_tag="ACHU9J_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1906719.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHU9J_003254"
     CDS             complement(4127..4852)
                     /locus_tag="ACHU9J_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR1906720.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHU9J_003255"
     CDS             complement(4874..5620)
                     /locus_tag="ACHU9J_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1906721.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHU9J_003256"
     CDS             complement(5627..6691)
                     /locus_tag="ACHU9J_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1906722.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHU9J_003257"
     CDS             complement(6763..7188)
                     /locus_tag="ACHU9J_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1906723.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHU9J_003258"
     CDS             complement(7185..7448)
                     /locus_tag="ACHU9J_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1906724.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHU9J_003259"
     CDS             7560..8060
                     /locus_tag="ACHU9J_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1906725.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHU9J_003260"
     CDS             8080..8274
                     /locus_tag="ACHU9J_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1906726.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHU9J_003261"
     CDS             8274..8564
                     /locus_tag="ACHU9J_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1906727.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHU9J_003262"
     CDS             8844..8996
                     /locus_tag="ACHU9J_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1906728.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHU9J_003263"
     CDS             9008..9373
                     /locus_tag="ACHU9J_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906729.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZPJP010000153         9490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPJP010000153 DAZPJP010000000
VERSION     DAZPJP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.154"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..270
                     /locus_tag="ACHU9J_003264"
     CDS             <1..270
                     /locus_tag="ACHU9J_003264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906730.1"
                     /translation="SPRNIAPHRITPAWCGYYDSIHLHFIPEMFAMKSAFRFFNSCYS
                     RFMSALDDASSSGCQYLLVEEIKTKMAAGCYPCGRFRAHIAITAA"
     gene            complement(285..392)
                     /locus_tag="ACHU9J_003265"
     CDS             complement(285..392)
                     /locus_tag="ACHU9J_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1906731.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(795..1895)
                     /gene="chaA"
                     /locus_tag="ACHU9J_003266"
     CDS             complement(795..1895)
                     /gene="chaA"
                     /locus_tag="ACHU9J_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1906732.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2165..2395
                     /gene="chaB"
                     /locus_tag="ACHU9J_003267"
     CDS             2165..2395
                     /gene="chaB"
                     /locus_tag="ACHU9J_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1906733.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2553..3248
                     /gene="chaC"
                     /locus_tag="ACHU9J_003268"
     CDS             2553..3248
                     /gene="chaC"
                     /locus_tag="ACHU9J_003268"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1906734.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3292..3645)
                     /gene="ychN"
                     /locus_tag="ACHU9J_003269"
     CDS             complement(3292..3645)
                     /gene="ychN"
                     /locus_tag="ACHU9J_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1906735.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3830..5224
                     /gene="ychO"
                     /locus_tag="ACHU9J_003270"
                     /gene_synonym="iatA"
     CDS             3830..5224
                     /gene="ychO"
                     /locus_tag="ACHU9J_003270"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1906736.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5225..5875)
                     /gene="narL"
                     /locus_tag="ACHU9J_003271"
     CDS             complement(5225..5875)
                     /gene="narL"
                     /locus_tag="ACHU9J_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1906737.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5868..7664)
                     /gene="narX"
                     /locus_tag="ACHU9J_003272"
     CDS             complement(5868..7664)
                     /gene="narX"
                     /locus_tag="ACHU9J_003272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1906738.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            8003..9394
                     /gene="narK"
                     /locus_tag="ACHU9J_003273"
     CDS             8003..9394
                     /gene="narK"
                     /locus_tag="ACHU9J_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1906739.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2302 a         2324 c         2443 g         2421 t
ORIGIN      
        1 agcccccgca acattgcgcc tcaccggata acgccggctt ggtgtggata ctacgactca
       61 attcatcttc acttcatccc tgaaatgttt gcaatgaaga gtgcattccg gtttttcaac
      121 agctgttaca gtcgtttcat gagtgctctg gatgatgctt ccagctcggg ttgccaatat
      181 ttacttgtgg aagagataaa gacaaaaatg gccgcaggct gttacccctg cggccggttt
      241 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
      301 caccagctga caatcgctgc ggtaataatc cccgccagga tcggtgctgc caggtcgtgc
      361 cagaaagtca tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacaag
      421 gcggctattc ttgaagtgtc tggtttcaag attagccccc gttttgttgt caggttttac
      481 ctctcaacgt gcgggggttt tctctttcca gcaaccaatg ccaccaggga taaagccccc
      541 gcaacattgc gcctcaccgg ataattccgg cttggtgtgg atactacgtc tcaattcatc
      601 ttcacttcat ccctgaaatg tttgcaataa agagtacatt ccggcttttc aacagctgtt
      661 gcagtggttt cgtgcaggtt gtggtacagg ctcgcaattc tgattaacga gctgtccagg
      721 tagtatgaag aaatacaata agcaggaatt tatcttatct cccccgattt ccgtcatcgg
      781 aggagatata actgtcaggc aaatatcgtc atcaaatagg cggcaaacag tgccagatgc
      841 gctgcgccat tgagcacgtt agtacgtccg gtggagaagg agatatggca cagcactaaa
      901 gaggccacca tcaccaccat ttctggcgca ccaagtgcaa actgtaattc gttacccgtc
      961 ataaaggcaa ttagcgtgac gacaggtacg gtaagcgaaa tggttgctaa cactgaacca
     1021 aagaacagat tcatcgcgcg ctggacctgg ttgttcaaca ctgcttttaa cgcacctaaa
     1081 ccttccggcg acagaatcaa cagagccacc aggaagccag taaaggcgac aggggcattc
     1141 atgctgtcga gcaatgtctc cagcgggctg gcattcattt tggtgactgc aataacggca
     1201 atcagatgga taatcaacca gatagcatgc cacacgctgc tatgggcaga cggtttaccg
     1261 tggtgcgggt cgtcgtcatc actgtcatct tcgtgctcgt agacaaacaa actttgatgc
     1321 gttttggtct ggatcagcaa aaatacgcca tacattgccg cagaaattaa tgctaccagt
     1381 aacgcctgac cggttgaaaa attcgccgca ggcagagcca ttggaaatac cagtacgatt
     1441 atcgccaggg ggaacagcgc aattaaatac tgcttgatac caaacagatt catatattgg
     1501 gtggcaaact tacgaccgcc caacaataat gaaaagccaa ccagcccacc ggtaacaatc
     1561 ataatgattg aatagagcgt atcacgcatt agcgttggcg cggcgtcgcc ggttgccatt
     1621 aaagctgaaa tcaaactgac ttcaagaata accactgaaa ggctaagaat aagcgaaccg
     1681 taaggttctc ccaggcgatg ggctaatacg tccgcatgac ggacaacact aaaggcgcta
     1741 cttaaaatac caataagcgc aagaagattg atggcaatga ccactggtag tgtctggctg
     1801 cttccccaca ggaacagcac taccagcgcc agaaccggga aaataagcga agtctccttg
     1861 tggcgggttt ttaccgcctc ttgagcattt gacattatgg ttatcccttt gcagatgaat
     1921 ttatcgaaaa tgtaaaaaat aggtaggaaa aataacagaa agtgtctgga tatcggtaac
     1981 attttacgaa tttttacccc gctgtcgatt ttttactcat tggggcataa aaataagtat
     2041 tacgtttaga caatgtttgt tttagcgttt cttaagaaca gtctgacatg aaaattctta
     2101 tgttttggca agagtagata ttgttgacca cacttaatgt tcaactttgt aaaaggagtc
     2161 aacgatgccg tataaaacga aaagcgatct gccggaaagc gtaaagcacg ttctaccgtc
     2221 tcatgcccag gatatctata aagaagcgtt caacagcgca tgggatcaat ataaagataa
     2281 agaagatcgg cgtgatgacg ccagtcgcga agaaaccgcg cataaagtgg cctgggctgc
     2341 tgtgaagcat gaatatgcca aaggggatga tgataaatgg cataaaaaat cgtaaaaccg
     2401 gtagcttagt taaagctatt cgtgcggtgt tgccttgcaa gtggtccgtg gattgcatat
     2461 tgtcccgtta gtggtttcaa aatgagcggt aaaaatgtcc ggaagacacc aaaaagttgt
     2521 cgcagggaag tatgcagtgg cggaggtgta aggtgataac gcgtgatttc ttgatgaatg
     2581 ccgattgtaa aacggcattt ggtgccattg aagaatcact cttatggtca gcagaacaac
     2641 gggcggcttc gctggcggcg acgctggctt gtcgacctaa tgagggaccg gtgtggatct
     2701 tcggttatgg atcgttgatg tggaatccgg cactggagtt taccgaatta tgcaccggta
     2761 cactggttgg atggcatcgc gcgttttgcc tgcgcctgac cgccgggcgc gggactgcgc
     2821 accagccggg acggatgctt gcactgaaag agggcggacg caccacaggc gtcgcctatc
     2881 gactaccaga agagacgctg gagcaggaac taaccctgtt gtggaagcgc gagatgatta
     2941 ccggctgtta tctgccaacc tggtgtcagc ttgatcttga tgatggacgc acagtaaacg
     3001 ccattgtgtt tattatggac ccgcgacatc cagaatatga atctgacact cgcgctcagg
     3061 tcatcgcgcc gttgattgcg gcggcgagcg gtccgctggg aaccaacgca caatacctgt
     3121 tttcactgga acaggagctt atcaaactgg gaatgcagga cgatgggctg aatgatttgc
     3181 tggtatcggt aaaaaaactg ctggcggaga attatccgga tggtgtgtta cggccgggat
     3241 tcgcctgagt aaacttccct catagtgggg cgtcagacgc cccctcaaac attaaaatgt
     3301 gagcacttta tcggctgaca gcgtccattg cgccagttcc acaagagtac cgatttccac
     3361 cccatcaata agaggaagtg tgctaatccc gcgcccgtcg gtacaggttt tgcacaattt
     3421 caccggtaca ttctgagcgg taaggatctc cagcatttgc tgaatgttgt agccttcccc
     3481 tggtttttgc ccgcgcaacc cggctgtgac cgcatcagac atcaggaaca gacgcagatc
     3541 cagattgctc tcctgctctc gtaacgcaat ggccagccgc aagctgttaa acaaggattc
     3601 gctcccgtaa ggtgcgccat tggcaacgat cacgattttt tgcattattt actcctgtat
     3661 tcagggaatt agacactcat cttctatctt actgcttctg cagcgtctga ccaatcggtc
     3721 acctttttaa ggattttcct gaaagcgcga gaaaatacga caaaagttgc cagtaatcgt
     3781 tattctttaa ggctatggtt tttcattatt accggaagtt accgacgttt tgagccgttt
     3841 cgttcctcgc attatcccgt tttatttact cttgctggcg gcaggcggta cagctaacgc
     3901 acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg atggtctgcc
     3961 ggatttaggc atggctcccg aaaaccatga tggggaaaaa cactttgctg aaattgtgaa
     4021 agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc aggcaaaagc
     4081 tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc acgtagagtc
     4141 ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg aaggacattt
     4201 caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt atctcacctg
     4261 gagccagctt ggtcttactc agcaggatga tgggttggtg agcaatgtgg gcgttgggca
     4321 acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca acttgctgga
     4381 cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt tgcgactatc
     4441 ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc aggaacaacg
     4501 gatggcgcgc gggtatgacc tgacagcccg gatgcgcatg ccgttctatc aacacctcaa
     4561 taccagtgtc agggtagaac agtattttgg tgagcgtgtt gatttgttta actctggtac
     4621 gggttatcac aatcccgtcg cgttgagtct gggattaaat tacacccctg tgccattagt
     4681 cactgtgacg gcccagcata aacagggtga aagtggcgaa aatcaaaata acctcggact
     4741 gaatcttaat taccgctttg gtgtgccgct caaaaaacaa ctttctgcgg gcgaggttgc
     4801 cgaaagtcag tcgttacgtg gtagtcgcta tgataatccg cagcgaaata atctaccgac
     4861 tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc cgtgggatct
     4921 aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg gtattcggca
     4981 actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac aagccaacag
     5041 cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg cgagcaatca
     5101 ctggcgattg tcggtggtgg tggaagataa ccaggggcag cgtgtctcct ccaatgagat
     5161 cacgctaacg cttgtcgaac cgttcgatgc attgtcaaac gacgaactgc gctgggaacc
     5221 gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga gacttgagct
     5281 tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg atatccaggc
     5341 ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata tcgcgctcgc
     5401 gtggggttaa ctggttaaca tcgcgctcag tagtggcacg gttagcgcgc aagctggcgg
     5461 ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc tgatgcaatg
     5521 ctttcagcag atcttccggt tccatatctt ttaacagata accatccgcg ccgcgtttca
     5581 gtgcggtgac cacatcttct tcatggttag agacgctgaa taccacaatg cgccctgaga
     5641 gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc atattgagat
     5701 ctaacaggat cagatcggga tcaagagatt ccgccagttc aataccctgt tcgccattac
     5761 tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt acgccagttc
     5821 gcagcatcgg gtgatcgtca atcagcagga tagtagccgg ttcctgatta ctcatgggta
     5881 tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac ttcggtgcca
     5941 cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg gtcgcgcatt
     6001 attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca gccgttatcc
     6061 tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac ttcactcgct
     6121 tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa gtggattgcc
     6181 tgatgcgaag gcaccaggcg aggcggcaat tgataatcca gcttcaccgg gaagccaaat
     6241 ttggcgctgt actcttcgca actcgcctcc agcgccggac gtaatccagg ctcggtgagc
     6301 tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt cagttcgtta
     6361 cggatctgac ttaacagttc gcggctgctt tctggcagcg catcgccctg catctgtaaa
     6421 caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg cagttcgcgc
     6481 gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg atggcgatcc
     6541 agcgccagcg tggcggtgag ttgttcaacc agggtatcca gcagttgttg ttgatcatgg
     6601 ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt atgagagtca
     6661 gccagccgcc acttcagggt cgtgccgcga tcaccaacgg gtaatacgcc gcgcgggcag
     6721 agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc ctgatgattc
     6781 tcttcatcat cagtgtcata cacccgcaat tcgatatcac gtagcagggt taaattctgt
     6841 aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg caaacggcgg
     6901 ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc ggttttctcc
     6961 tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat attgttcaat
     7021 gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt tcgcgcgttg ggtaaaatcg
     7081 cgatgactga cggcactcgc cattgccagc agttgccgcc acggttgtag cagtcgcgcc
     7141 cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat tacccgatgg
     7201 accagtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga taccagctga
     7261 tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttcgcggtt ttgtgcacgc
     7321 atcagtgcag ggatcagttc attacgccag taatcttgta aaccctgtaa ttgcgccagt
     7381 tgttcgtctc gttctgctgc tcgagttaac tcggcgctaa atgccgtttg ttccatctct
     7441 ttaattaagg gcttgtcttt ctcacttaat ggcactgccg ccaacagacg gtaactttgc
     7501 atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc ttgcaccagc
     7561 cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac aataagcgca
     7621 acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgcaaac ctcttccttc
     7681 aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa tggcagtatt
     7741 ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat ttgccgtgtg
     7801 gttagtcgct ttacatcggt aagggtaggg attttacagc accgtgaaaa atctcataat
     7861 ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata gataaagtta
     7921 tcttatcgtt tgatttacat caaattgcct ttagctacag acactaaggt ggcagacatc
     7981 gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg ctactggagc
     8041 tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg gtcaacgtat
     8101 tgccagccgt aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt gcgtatggat
     8161 gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta cgaccgatca
     8221 gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg ttccatactc
     8281 ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg gtattctgat
     8341 tattccttgc gtctggctgg gttttgccgt gcaggatacc tccacgcctt atagcgtctt
     8401 catcatcatc tctctgctat gcggctttgc tggcgcgaac ttcgcatcca gtatggcaaa
     8461 catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga atggtggtct
     8521 gggaaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat cactgtcgat
     8581 tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc tgtatctggc
     8641 gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg cgtggtttgg
     8701 catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg tactcaaacg
     8761 gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct ttatcggctt
     8821 ctccgcgggc tttgcgatgc tgtcaaaaac gcagttcccg gatgttcaga ttctgcaata
     8881 cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg cattatctga
     8941 ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga ttttcagcgg
     9001 cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg cgttcttcgc
     9061 ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt tccagatgat
     9121 ttcagtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg gttctgacga
     9181 acgtgcgatg cgtgaagcgg caaccgacac ggcagcggcg ctgggtttca tctctgcgat
     9241 tggcgctatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg cattaacggg
     9301 ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg tagtgattac
     9361 ctgggcggta tatggtcggc attctaaaaa ataaatctta atatttgatt atcctttgcg
     9421 cggcatgatg tcgcgctttt tttatgcgtc atttagttac aacatactca tattatatgg
     9481 cttatttcgc
//
LOCUS       DAZPJP010000154         9424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPJP010000154 DAZPJP010000000
VERSION     DAZPJP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.155"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHU9J_003274"
     CDS             <1..173
                     /locus_tag="ACHU9J_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1906740.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHU9J_003275"
     CDS             complement(345..674)
                     /locus_tag="ACHU9J_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1906741.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHU9J_003276"
     CDS             complement(679..864)
                     /locus_tag="ACHU9J_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1906742.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHU9J_003277"
     CDS             complement(861..3500)
                     /locus_tag="ACHU9J_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1906743.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHU9J_003278"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHU9J_003278"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1906744.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHU9J_003279"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHU9J_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1906745.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHU9J_003280"
     CDS             complement(5227..5493)
                     /locus_tag="ACHU9J_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1906746.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHU9J_003281"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHU9J_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1906747.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2343 a         2461 c         2305 g         2315 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcat
//
LOCUS       DAZPJP010000155         9401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPJP010000155 DAZPJP010000000
VERSION     DAZPJP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.156"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHU9J_003282"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHU9J_003282"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1906748.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHU9J_003283"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHU9J_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1906749.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHU9J_003284"
     CDS             1393..1914
                     /locus_tag="ACHU9J_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1906750.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHU9J_003285"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHU9J_003285"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1906751.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHU9J_003286"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHU9J_003286"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1906752.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCCEGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHU9J_003287"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHU9J_003287"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1906753.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHU9J_003288"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHU9J_003288"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1906754.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHU9J_003289"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHU9J_003289"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1906755.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHU9J_003290"
     CDS             complement(6556..7449)
                     /locus_tag="ACHU9J_003290"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1906756.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHU9J_003291"
     CDS             complement(7446..8099)
                     /locus_tag="ACHU9J_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1906757.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHU9J_003292"
     CDS             complement(8110..9369)
                     /locus_tag="ACHU9J_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1906758.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2666 c         2493 g         2150 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ctgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aaaccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa c
//
LOCUS       DAZPJP010000156         9395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPJP010000156 DAZPJP010000000
VERSION     DAZPJP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.157"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..2180)
                     /gene="traC"
                     /locus_tag="ACHU9J_003293"
     CDS             complement(<1..2180)
                     /gene="traC"
                     /locus_tag="ACHU9J_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR1906759.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYR
                     VFFSYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGA"
     gene            complement(2306..2668)
                     /locus_tag="ACHU9J_003294"
     CDS             complement(2306..2668)
                     /locus_tag="ACHU9J_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906760.1"
                     /translation="MKSIYLESVLAFIFVGVMAMLICGLFYNDYLEQQPATPEQLTEI
                     TQDTPCAAEAFKEAIKSDTSDYQPEPLSLGKAKELASACRERNEMAEVKRVRENERNK
                     IREKQLQALNDAHSVKER"
     gene            complement(2699..2910)
                     /locus_tag="ACHU9J_003295"
                     /pseudo
     CDS             complement(2699..2910)
                     /locus_tag="ACHU9J_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2990..3466)
                     /locus_tag="ACHU9J_003296"
     CDS             complement(2990..3466)
                     /locus_tag="ACHU9J_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906761.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNCLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(3459..3680)
                     /gene="traR"
                     /locus_tag="ACHU9J_003297"
     CDS             complement(3459..3680)
                     /gene="traR"
                     /locus_tag="ACHU9J_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR1906762.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(3815..4330)
                     /gene="traV"
                     /locus_tag="ACHU9J_003298"
     CDS             complement(3815..4330)
                     /gene="traV"
                     /locus_tag="ACHU9J_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR1906763.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(4327..4578)
                     /gene="trbG"
                     /locus_tag="ACHU9J_003299"
     CDS             complement(4327..4578)
                     /gene="trbG"
                     /locus_tag="ACHU9J_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFR1906764.1"
                     /translation="MNNLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFMNCDEKGEGQ"
     gene            complement(4571..4891)
                     /locus_tag="ACHU9J_003300"
     CDS             complement(4571..4891)
                     /locus_tag="ACHU9J_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR1906765.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPAKELIDRIIQKYKE
                     PFDE"
     gene            complement(4878..5468)
                     /gene="traP"
                     /locus_tag="ACHU9J_003301"
     CDS             complement(4878..5468)
                     /gene="traP"
                     /locus_tag="ACHU9J_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR1906766.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTAFWATLALMYVSLAAITGKYPVRPGKMKCVRVGTADEHLK
                     EVYTEDTSLSEKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(5458..6885)
                     /gene="traB"
                     /locus_tag="ACHU9J_003302"
     CDS             complement(5458..6885)
                     /gene="traB"
                     /locus_tag="ACHU9J_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR1906767.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMAGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(6885..7613)
                     /gene="traK"
                     /locus_tag="ACHU9J_003303"
     CDS             complement(6885..7613)
                     /gene="traK"
                     /locus_tag="ACHU9J_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR1906768.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(7600..8166)
                     /gene="traE"
                     /locus_tag="ACHU9J_003304"
     CDS             complement(7600..8166)
                     /gene="traE"
                     /locus_tag="ACHU9J_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR1906769.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(8188..8499)
                     /gene="traL"
                     /locus_tag="ACHU9J_003305"
     CDS             complement(8188..8499)
                     /gene="traL"
                     /locus_tag="ACHU9J_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1906770.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFALPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(8514..8873)
                     /gene="traA"
                     /locus_tag="ACHU9J_003306"
     CDS             complement(8514..8873)
                     /gene="traA"
                     /locus_tag="ACHU9J_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR1906771.1"
                     /translation="MNTVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(8906..9301)
                     /gene="traY"
                     /locus_tag="ACHU9J_003307"
     CDS             complement(8906..9301)
                     /gene="traY"
                     /locus_tag="ACHU9J_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFR1906772.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVEGLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
BASE COUNT         2276 a         2559 c         2152 g         2408 t
ORIGIN      
        1 gcaccggtat gacggcgggc ggtacggtaa cctttctcaa taaactcacc gaccttgcga
       61 tttttgaagt ccagcagacg ccagccttca tcaataacgt tcagtttctt gaggttacgc
      121 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
      181 agtgacggac ggtcttccag tccgcccagc tccagcacca ccattttcgc atcatcccgc
      241 agggacggct catcggagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
      301 agcagcacaa tcatttcgtc cagacggctg cggatggtcg gtgacccggc atactgctcg
      361 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
      421 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
      481 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcaata
      541 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgccaccc
      601 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
      661 gagtccagca cgctgcggat aagtggctgt atcagcccgg ttttaccggc cccggaggtg
      721 ccacagaccg ccatgttgta gttggtgtta ttcatccccc ggaagaaaat atcgataaac
      781 gccagctggt tacggtaggt gggtgccagc agacctgccg gtgtcagggg gttatccgcc
      841 actaacggca tcaggttggc aacattaaag ctctctgcgc gctgaaccac tccggcctct
      901 ttcagctgtt taaacaaccc tttcccggcc ataaagggca gacaggtcag gaaattgcgc
      961 atgtggttaa agcgcggtga aatcagctca aaaccgtttt tacggaagct gttcaggatg
     1021 tcctgctcca cttccagcgc cgtttcattg ttgtccttgc agaatgctgt gatgttgagg
     1081 aagtaggaca cgacagaaga ctgaccggag cccagacgct gacgcagttc cccccactcc
     1141 ttcgcctctt tctccacgga cggaaaccat ttggcatagg aggtcttcga ctttttctcc
     1201 aggtccatgt acttcaggtt ggcttcgctg tgggttttca cctggtcttc caccaccaga
     1261 gtcagcgtca ggatgaacgg acaggagatg gacagttccg ggttcagcag gttgctgtag
     1321 ttgtcggcca tattccacag gaaggcgatt tccgggttac gggccagatg gaaattcagg
     1381 atgcgggccg tgctgttcct gccgttctca cgcaggccca gcgtcaggta atcagcccgg
     1441 actttcaggt caaaactgtc ctccacacac tgataattca gatcagaata cgggtccagc
     1501 tgacgtcttt tcgggtacag ggagtccggg ttatgattaa tcatctcccc gacaatatcg
     1561 ataaaggcct gtgcatccac cgtctgtgtg gcgatactgg ccccctgtaa cgacgcccgg
     1621 atgattttca ccaggttttc catttccaga atgtcggccc ggcttttttt ctttgaggga
     1681 gaacagtacg agaagaatac ccggtaatgc cgcagggtca ggggcagatt catcccctcc
     1741 ggcagcggaa actgtgtcgc tgccgcgttc atataatagg cccgggtaat ggcgttaaac
     1801 cgttcagcct gttcacctga ccaggagaac tcacgcagcc cgtattcaat tctgtcacca
     1861 accagctggc tggacatcag atggatgcag aacggcaccc cgcgcggcag tttggtgcgc
     1921 agcatgtggt cgagcgcctc cacaatggac tcattcgccc cattgatggg aatggcttcc
     1981 agcataaagc ccatcgtggt gtcattcatg aacaggccgg actcctggtt gtaatcacgg
     2041 tacggcagca gacggctgaa ctgcgggaag ctcatttcgc ccagaacttc attggccttt
     2101 gcggattcgt caggcagttt cagtgctgtg acgagggagt taaccgcctg agtgacggcc
     2161 tcaagtgggt tattactcat atgagtattc cttagtgcgt ttaaaaaagg aaaatatcag
     2221 tctgtggccg tggatatatc acgcagtgcc attcctgaaa ccacagcaca aataagagat
     2281 ggaggctttt aacaggaaac caggattaac gttcctttac tgaatgagca tcatttaatg
     2341 cctgaagttg tttttcacgt atcttatttc gctcattttc tcttacccgt ttcacctctg
     2401 ccatttcgtt tctttctcta cacgctgatg caagctcttt tgccttacca agacttaaag
     2461 gttcaggctg ataatcagat gtgtcagact taatagcttc tttaaatgct tctgctgcac
     2521 agggggtatc ctgtgtaatc tctgtcagtt gttctggtgt tgcaggttgt tgctcaaggt
     2581 aatcattgta aaaaagacca catatcaaca tagccataac gccaacaaat ataaaagcca
     2641 gaacagattc taaatatatc gacttcattt cattttcctt tgaagcagta agcactcatt
     2701 accccccatt cagtgtatcc atcacgttgt tcaaaggttt atttgtttta acctcaatac
     2761 attcccctgt atttccaaag cccctgcctc cgtgtgcttc gattgtccga agaacctgca
     2821 cgcatacatc cgtatttggc atggggtaac attccagctt ccgccaccgt taatattttt
     2881 tatcagcaca agaaaaataa aggtatgcat tctttattac ctccgcttat atattaatca
     2941 ctgaatatcc tttcagttaa cttatgaggt cataaaacac tgcagattct cattcggatt
     3001 tattcccttc agtaaacttc cgaatagtat cctgttcacg ctgacgggtg gcagactcct
     3061 gcattgcatc catcttttcc caccagagac tcctgatttc cctcttgtcc agaccggtca
     3121 gttttttacc ggacagcagg cagttaagca gttcctggtg catatccgga cagtcttcag
     3181 tcagactcat aagccagagc aattcctgac gactgagtac cctgcgtgat tcctccggta
     3241 cgctgtaata attaaaaata cggctgaata ccccacaaca gcaggcaacg gacaggcaca
     3301 gggatttcag atacaggcca gaaacacaga atgtggcaac accacaaaga atagctataa
     3361 ctaaaaagcc cttacccagt gccatacaca aagaaccctg ttttctgaat atccgctcat
     3421 gctcatccag aataaaatct attttttcat tttctgattt atgcatagtg tttcctctgc
     3481 ctttcctgat acgcctgaca ttcaacgcac aacgtcacac cgggaaatat tttccgccgg
     3541 gcttccggaa taggatttcc gcatgcttca cagagataaa caggaatacc atgaacattt
     3601 attttctggc gtatccggtt tattcctgtc atggtcagtt gttctgtcac tgaatatgct
     3661 tcatcggctt catcactcac ggaatattcc tcccgacatg ttctgacacg ctaccgccca
     3721 tcccttttca cgcacagggc actcgtttta caggcagatt ttgaatacag aaagcccgcc
     3781 ggcattgccg gctgatatac aacttcaggg ccagttaatt aatacgtggt tttccccacg
     3841 cagacggttt aataacaaaa aacacactgg atggctgatg ataaacatcc tgattatcaa
     3901 tataaggcgc tatccataat gcagccgtct gctcacccgt tcttagcgga cggggaggtg
     3961 ttaccggcac aactgaactg accggaacaa ccgttttcac ttcccgggca gcggtaaaca
     4021 gcgggcgtgg tgccagcagt ttctgctccg gatgtgctgt caccgcaggc ctgctgcggg
     4081 acggcgttgt cgccgcaacg gcctgcaccg acgtggtccg gaagttccct tcagccaggc
     4141 gcggcagtga tgccgcaacc ggctttgctt ctgaggactg ctccagtttt ttggccttct
     4201 cattagcctg ctccatcgtc atacaggtat cggatgtggt ggcgttacac tcaaattccg
     4261 tactggttcc ggcacagccg gaaagtaata aggtccccag cagaggaata aataaagaaa
     4321 tctgtttcat tgtccctcac ctttctcatc gcagttcata aaatcattct tcaaatccct
     4381 gaccgtacag gtttttacaa tgtcaggatt ttcaaaaacc gtattccgtg atgatttata
     4441 aacatatgcc acaatgcgtt ttccacccgc atcaggttct ggtgcactga cttcacataa
     4501 cggaggtgtt attccggact catacagaac cggataattt atttttttga cgctgccatc
     4561 agaaaccaga ttattcatcg aagggttcct tatatttctg aataattctg tcgattagtt
     4621 ctttcgccgg aacagatttt ccgccaccgg attgtccggc aaccacaata tgccccggag
     4681 aagaaaataa gctcatcgac attcccttct tcatcacacc gacagcagcc atattaaaat
     4741 tgtgcggcaa tgattcagaa ctgaacacct tcggagcagg aagaactatt tttgtggtgc
     4801 agttactgag aacagcatgc ataaaatcat ctgatacgtt tttatccggc acaacgcggg
     4861 ctgtaataac attaatatta cgcatgttca tttttatctc cgttgcttct gtttgttcgg
     4921 tcatcaggga aataaacatg gcatttcctg attttttccg acagagaagt atcttccgta
     4981 taaacttcct tcagatgttc atctgcggtt cccacccgga cacatttcat ttttccgggt
     5041 cgcaccggat atttcccggt gatcgctgcc agcgagacat acatcagtgc cagtgttgcc
     5101 cagaatgcgg tgaccagttg catcagtgac cggtcagtct ccctgacata atctgcagca
     5161 tcagccgcgc ggtaatggca gatatcctgt atttctgatc tgcctgaaga atcagaggaa
     5221 gaaaaccagc attcactgac cgggaactgt ccggcaggtg ctgtttgtct tacgaagtta
     5281 atctccttaa ccagtaattt ccccggcgtg gtgttgtcct tccataaaac aaacagagcc
     5341 atcagggcca ttgtggccag tggcagaatg acggtgtact tcagacacca gaacagtcct
     5401 cgcagaaccc gggcgacaac ataccgggcg gcatggcacg cctgacggga cgacatatta
     5461 tttgccatcg ttgcctcctc cctggctgcc ggacataaac tgctgcggat tcagattgcc
     5521 cagctgcttc agcatctggt cggtgttaaa accgttcagg tggctttctg ccgagggagg
     5581 attcggcaca gggctttccg ggttaccctc ttccgctgta ctctgtgact gctccagcgc
     5641 catttcctcc acggttttca gctggaagcc gtcctggaac accactgtca cttcgttgcc
     5701 cgcaccaatc ggtatcaccg ggtgatactg ttcggcacgt ttgatgtagt agtcactgag
     5761 cgtctgtgcg gctttcgatg cgccgccacc gatacccatt ttaaggacat cgccggcccc
     5821 gtaagcagca gtggcaccca gcccgacagc cggctgggag gcgcgctcca ttccctgacc
     5881 gataccgtca acaaatcccg cgccccatgc ccagccgagg attttgccgt tacgcatcac
     5941 cacttcgccc ttgataccgt ttttaccccg gaagctgaca tgccccttaa tcggcatatc
     6001 aatagttttg ccgtccttca ggcagctaat attgcgggtg cgtacaatgg cacgctcact
     6061 ggacacatcc ccccaggctt ccagacccac aaaacatccc gttgcgtcat acgtcttgct
     6121 gttcggcatt tccaccagac cggtgatacg cagctgcatc ggcaccgtgg attcattacc
     6181 ggtgactgag gcattggcat ccgccccttc aatcagcatg gctttcgcaa aacttcctga
     6241 cggaatgtac ggcagcgatg gtccctgttt tccctcatta cgggtaaaca ccttacgctg
     6301 tatccggtta ggcaccggca cagactggta cgtcacctgt ggtggtggcg tgacaccatt
     6361 cccgggataa aacgccgtcg gcggtggaac agcaacgctg ccctgcggcg ggaaggatac
     6421 gggggtgttt cctggctgtg gttcgccttc cggcccgggc ggtgaggctg gcatctgcgg
     6481 tacaggctca ccggtcgcgg tgacgggatt tgcacccagg gcttttacct gctctgccag
     6541 ggcagcatta tcctgtccca gcttttcaat acgacgctgg tcatcaccgc gctgtttgtt
     6601 caggacatcc agttcacgac ggatttcctc atactgcttc tgcatctgcg ctgccgtcac
     6661 ctgcatctct gtggtggcat gctgacgcac cttgtcatca aaggtcgtat ccaccacacc
     6721 ggtcatatcc ggcacaggct cctgttcagc caccgcctca ccgttaccgg ccatgtccac
     6781 atcagacaga tacagtgcac caccaatcgc ggaggctgca ccgacaacca caatccccag
     6841 ccacaggtac tgcttgcgtt tcacaatggt attgatactg gccatcagtt gccctccccg
     6901 tcacggatga catacacatc catgcgcgca cctgccagta actggctggc aggctggctg
     6961 aacatcacgg cacgggttcc cggctgccag aaatcacttt cccggatatt cagggcagac
     7021 agcgttttat tttccacggc aaagcggacc atcttcaggt gattccccgt ccatacggca
     7081 tctgccactg aagacagccc cgccggggcc tgcagggttt cctttgtcac aggaacctga
     7141 taccagcctg caggtaattt tccgccacgg acagcctggc tgatggtcac aagcagggat
     7201 tcgtaaggcg tggacgtttc ccacgcaccg gcttcttctc cggttccgcg caggtcactg
     7261 accagctgaa tggtacgccc cgcgccttca cggggaacgg cctgaatgga aagattcaga
     7321 ccacgttctg tttccagaat gaacgtaaag ggctttttgt tgacggtggc aaccaccaca
     7381 ccgccggagg cggtctgctc attattggtc agggcaccat ccagactgtt cacggcgata
     7441 atccggtcac cgggaacggt aaacagattc gggctggtat tgctgatggc aatactggcc
     7501 tgaccaccgt tcaccattgg caccacggtg ggggccagcg taccgtttgc ggccatcacg
     7561 ctgcaggaaa aaaacaggct gccgaatatt attgccgtat tattttttct catcgtctgt
     7621 ttccccgaaa ttatccagcc aggtcacccc gttttcccgc ttcagaataa ggatgtaatg
     7681 tttgatatca gtgaaaggtt ttgaatcacc aatccaggtt ttaagcacac cacgaatttc
     7741 cacacggcca tactgaggcc atacacggac actggtctgg aaaaaggctg agttcacgtt
     7801 atcgttttta atacgcttcg cttcttcagc cagaataact ttcatctggt tctgtgcgcc
     7861 cgggcggata tattgcagaa gcgcctgatg tgaggcatcg acggtttctg atgaaacatt
     7921 cagacggagg gcaataaatg acagcgccat ctgctgtaaa taagaggcgt cggcactgtt
     7981 ctgtgacacg gcaaagggaa cattaaatcc cattggggtc acggcagtgc gttgctcatt
     8041 ctgaagacgg tagttattca ccccctgaat gacgttaaca gagaggctga gaacaataag
     8101 cactgacata aatataaagg cgatggccat tacacgactg gtacttaaac gggcaccgtg
     8161 ttccatataa caatcctggt atcagttcta tttaatccac tgccggaaac acgaatcggg
     8221 aacattatga aaaataccgc gcaacagggc tgttggcata taccagtaaa tcaggtcacg
     8281 taaccagaaa ctgccccgcc cttttttcag ttttttaatc ccgaaataaa ccagaaccgc
     8341 tgcaccaata ccgaacagat atttcgatgt tgtgataccc cagccaatac agattgcggc
     8401 ggggatcagt tcatccagtg gcagggcaaa ccagcggctc tggttggtca gtgtttccgg
     8461 gaaacgatat ttcttaagtt tattctcgtc tcccgacatc gttttatttc ctgtcagagg
     8521 ccaacgacgc tcatacccac agcaataaat acagagatga tggcaaagcc gaccaggaac
     8581 ttgacgtttt tggtcatcat gtacatgacc gcaccgacca gcacctcagc cagaacaacc
     8641 catttaacga tactggagtc cttaccgaag gtcgccttaa ccgtatcgtt acctttagcc
     8701 atcaggtcgc ctttaccagc agccatagct aactcaggga acaacatcat cagaacagca
     8761 accgggatca ctgcacgaac caggcgaagc atattcagac gagtgaattt agaaaaaaac
     8821 gacttctttt tgacgggcgc agaagcaccc tgaacactta aaacagtatt cataagttac
     8881 ctcaatttcg gataaatgaa taaaactaga gtgtattaaa tgttatatct tcctctttat
     8941 ctgcctccct gaatatctcc gagttataaa aatcaggaaa cttgatcaaa tgatcaataa
     9001 ctctgtctgc agcctcatct gttttacacc atccactacg attttttgct gcaataagta
     9061 gctggttagt ttcatcatcc agacgcattg tcagatactt aaccagcgac ttaccataga
     9121 tattccctgc cctgttaaac tttggatagc ggtgaagatg atctttaagt cttattactg
     9181 cctcaaacga tcgacttctt ccgcttctgt tacttgcctc aattaataga ccttccacat
     9241 ctacaggtaa ttttagtttt accatcttac ctgttgcaga acgtgtacca aatcttttca
     9301 ataacacctc ccgctgttta tcttctggtt actacttatg tttgcagaat aatcagaaaa
     9361 ggtaaataga cggaaagtct atattttgtt aacac
//
LOCUS       DAZPJP010000157         9359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPJP010000157 DAZPJP010000000
VERSION     DAZPJP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.158"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..815
                     /locus_tag="ACHU9J_003308"
     CDS             <1..815
                     /locus_tag="ACHU9J_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR1906773.1"
                     /translation="NALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIYLPPLTR
                     GNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGNDPIIGI
                     EAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHU9J_003309"
     CDS             complement(956..1729)
                     /gene="uxuR"
                     /locus_tag="ACHU9J_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1906774.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1944..3404)
                     /locus_tag="ACHU9J_003310"
     CDS             complement(1944..3404)
                     /locus_tag="ACHU9J_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1906775.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHU9J_003311"
     CDS             complement(3485..4669)
                     /gene="uxuA"
                     /locus_tag="ACHU9J_003311"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1906776.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5009..6352
                     /gene="gntP"
                     /locus_tag="ACHU9J_003312"
     CDS             5009..6352
                     /gene="gntP"
                     /locus_tag="ACHU9J_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1906777.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6516..7418)
                     /gene="fimH"
                     /locus_tag="ACHU9J_003313"
     CDS             complement(6516..7418)
                     /gene="fimH"
                     /locus_tag="ACHU9J_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1906778.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7438..7941)
                     /gene="fimG"
                     /locus_tag="ACHU9J_003314"
     CDS             complement(7438..7941)
                     /gene="fimG"
                     /locus_tag="ACHU9J_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR1906779.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7954..8484)
                     /gene="fimF"
                     /locus_tag="ACHU9J_003315"
     CDS             complement(7954..8484)
                     /gene="fimF"
                     /locus_tag="ACHU9J_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1906780.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8494..>9359)
                     /gene="fimD"
                     /locus_tag="ACHU9J_003316"
     CDS             complement(8494..>9359)
                     /gene="fimD"
                     /locus_tag="ACHU9J_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1906781.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2211 a         2462 c         2360 g         2326 t
ORIGIN      
        1 gcaatgccct tcagagtcaa attattacag ataatcactt ccttcatcac ccaatagtcg
       61 acagtgagtt tacacataaa ttaaaatatg cacgcatgga tttggaaaac atctatcttc
      121 cgcctcttac tcgtggcaat aaccataatt acgatggaaa aagcgctgtc gaaattagaa
      181 aacttgatat tagtaaagag tcctggccat ttaattatgt gacaggagca tgcagagaat
      241 ccgatggtat tacaacaaca ggacgtatgc tctatcggaa cttgaaaatc acctcagctt
      301 tagacgaaat atatggtggc atatgtaaaa aggcgcatgc tgccacagag ttagcagaag
      361 gtttacggct aaatttattt atgaagagcc ctttcgatcc tgttgaggac tatacagttc
      421 atgaaataac attaggccct ggttgcaatg ttcctggtta tgccggaacc acgataggat
      481 atatttcaac ccttccagct tctcaggcaa aaagatggac caatgagcag ccaaggattg
      541 atatctatat tgatcagata atgacggtaa caggggtagc aaattcttcc ggattcgccc
      601 ttgccgcatt acttaatgct aatattgagt tgggtaatga cccaataatt ggtatagaag
      661 cctatcccgg taccgcggag atccatgcaa aaatgggata taaagttatc ccgggtgatg
      721 aaaatgcacc actcaaacgt atgactctgc aaccgtcatc tcttccagaa ctgtttgaat
      781 taaaaaatgg tgagtggaat tatattggta aataactacc aggaagaatg agtactaaca
      841 ttcaggttat acagatagct aaacaatacc ctgcaattta caaagtaata ctcattgcga
      901 cttcttaatt taagaaagcg caaggaacat ttacccttgc gcttattata ataagtcagg
      961 cgtcaacttt atccagcggc catgaatcaa acagatagcc atcaaaataa atatcgtcaa
     1021 cgttcgagaa ttccagcaga cgttgcttaa cgttttccag atgccgccac attgccagct
     1081 tcgccgctcg ggcatctttt ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc
     1141 actctttgcg atacaggctg tcatccagat ggctgtgcaa ctggatccac attggattgt
     1201 tttcccgcca ctgccaggac tgacggaaca gctccaccag catgctgtta tgcgttgctt
     1261 cggcaatggc gagatggaac tgcatatcac cactttcgct gctgcccggc gcgctggaag
     1321 ccagctcacg ctcttccagt tgcaatgcct gacgcatttt gacgatatct tcgcgggtag
     1381 cctgcaaagc ggcaaattcc gcgatgttac tttccagtaa ctgacgcgcc tgtaacagct
     1441 caaaaggacc ggcatcattg cagacgttag catccggact gtctgtgttg tggctgcctg
     1501 agctatcaag aacatagata ccggcacccc ggcgcacttc caccagccct ttgatctcca
     1561 gcatgatcag cgcttcacgt accaccgtcc tcgtgacatc aagcatttct gcaatttcac
     1621 gctccggcgg cagccgttcg ccaggattgt atggcgtctt tatgatcaga tcgcggatca
     1681 tcgccccgac ttcctggtaa ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc
     1741 cgttaggtgc ggttaatcaa atttttgccg gatgcggcgt gaacgcctta tccggcctac
     1801 aacgggcaat tttacggcac ccgttaatct caaactatac cattagcact ggaagttggc
     1861 acgtacaggt gggcaaaaaa acgaaaactg gtcaaccaaa tatcagtttg attgaccagt
     1921 aagtctgtag ggtaatcagt tagttacagc gcagccacac actcgcgcgc accgcgttcg
     1981 cacagctgct gatatgccgc cgtcaccgcg ccaacaaagt cggcattctg cggcagatca
     2041 tcggcaaaaa tgccgctcag gcccagcagc gctttcacgc ggtctgcgcc ctgatactgc
     2101 gcgttaatct tctggaactc ggccagcatc gggtcaacca cgtcaatggc attaccctgt
     2161 tcatccacgc cctgggtgta gcgcatccag ccagccacgc ccagtgccag gtgacgccag
     2221 ctgccgccgt tttgcaggtg cagacgcacc ggatccagaa gacgctgcgg taacttctgg
     2281 ctgccgtcca ttgcaatctg ccaggtacgg tgacgcagag acgggttgct gaaacgctcg
     2341 atcagcagcg tcgcataggc gttcaggtct gtaccttccg gcatcgacag cgttggcgct
     2401 tgttcctgca tcatcagggc aaaggccgct ttgcgataat ccggattagt catggtgtcg
     2461 gcaatggttt catagccgcc gaggtaaccg aggtacgcca ggaaagagtg gctgccgttc
     2521 agcatacgca gtttcatcat ttcgaacggc acaacgtctg caacgaactg tgcgcccact
     2581 ttatcccagt ccggacgacc attaacgaag ttatcttcaa tcacccactg acggaacggt
     2641 tcgcaggcaa tggcgcacgg gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt
     2701 tctggcgtcg ccgccggaac gatgcggtca accatggtgc acgggaaggt gacgttctct
     2761 tcaatccatg ccgccagctg cggatcacgt gcctgagcca gccccagaac cgcgaccttc
     2821 gccacatgac cgttttcacg cacgttgtcg caggacatca ccgtaaacgc tttcagccct
     2881 ttttcacgac gcagacgcaa ggcttcgacg atgtaaccaa tcgcggactt cggcgcagtc
     2941 gggttttcca gatcgtgctt gatcagcggg ttattgagat ccagctgacc gcttgccgca
     3001 tcggcgcaat aacctttctc tgtgaccgtc agggagacaa tcgccgtctg cggacgcgcc
     3061 atcgcgttga gaataccttc acaaccgtcg atttccggat gcagggcttc tttcatcgaa
     3121 ccgataattt tcagctcggt gctttctgcg cctttttccg ccacggtgta cagcagttgc
     3181 tgttttttca ggttttcaat cagcacacgg tcgttgcctg gcatcaggtt aacttcgcag
     3241 ataccccagt cgctgtcggt actttccagc agatgatggg tgtacagcgc ctggtgcgcg
     3301 cggtgaaacg ccccgcaacc gagatgcaca atgcgtgatt ccagacgaga atgatcccat
     3361 gacgggcggg caaccggcag attgctgtca acaatagtag tcatcgtaaa ctccagaatc
     3421 cgtcttcaga gagtgacggc aaggaagaag gaaccggcac ggcgcgcagc catgccggtg
     3481 ggtatcaacg gctaaagaaa gcgcgctgga tcgccagttc gacaccgcga acttcagcca
     3541 ggcctttcag acgaccaatt gcggagtaac ctgggttggt tttcttcttc aggtcgtcca
     3601 gcatctgatg accgtggtcc ggacgcatcg ggatcaggtc ttctttgcct tccgctttac
     3661 gacggtgttc ttcttcaaca atcgctttca ccacttcgta catatcaacg tcaccgttca
     3721 ggtgcgccgc ttcgtggaag gttttcgggt tatcttcacg catggtggag cgcagatggg
     3781 tgaagtaaat acgcggaccg aactgcttga tcatatcaac cagatcgttg tcagcacgca
     3841 cgccgtagga acctgtgcac atggtgaaac cgttcgccat gctgtttacg gtatcaacca
     3901 tccactgcat atcttcaatg gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg
     3961 gatcgtccgg gtgaacagcc atacggacgc caacttcttc ggcaaccgga ataatcgctt
     4021 tcaggaagac ggcaaagttt tcgcgcagtt tggctttgtc gatatcttta tacagctcca
     4081 ggtgcttacg gaactggtcg agggtatacc cttcttccgc gcccggcaga ccggcaatga
     4141 tgttacgggt cagacgcgct ttgtcttcgt cgctcatagt ggcgaagcgt tcagcggcct
     4201 gagcaatttc ttcttcggtg taatccgctt ccgcgcctgg acgcttcagg atatgcattt
     4261 cgaatgcagc gaattcgatc tggtcgaagc gcagagcttt ggagccgtct ggcagcacgt
     4321 attcgaggtc agtacgggtc cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa
     4381 tgccgcactg cgccaggttg cgcagagtct gctgatagtt agcgatccac tgttcatagt
     4441 tgccagtgtg ggttttgata tcttcgtgaa ttggcacgct ttctacgaca gaccatacca
     4501 ggcctgcgtc ttcaacgatc gccttgcgtt tgaggatctc ttctacggac catacttcgc
     4561 cgttcgggat atggtgcagc gcggtaacca cgccagttgc gcccgcctga cggacatcag
     4621 ctagagaaac cggatcgttt gggccgtacc agcgccaggt ctgttccatg atttttcctc
     4681 tttcacatag tgggttccac agcaaagaga cgtggagccc cggagttgtt taggtatccg
     4741 gatgaacaac cagaccgcag ccgtggcgtt gcatcggatg agatggcgta taagttctac
     4801 cttttggtaa gacatgtcaa cccgaaatct gaaattggtt aaccacatcg caagaatttc
     4861 actctcacgg ttgttgctgc aaattgcagc aaagggtaat cgatggttat tgatcagagg
     4921 atgtgacatt catcgcaaca atggttgacc aatttacata acatgtcgcc caaaataaca
     4981 acggttcaac caggtaaggt aaaacgttat gcatgtgctt aacattctct gggtggtatt
     5041 cggcattggc ctgatgctgg tactgaattt gaagttcaaa atcaattcaa tggtggcttt
     5101 gctagtggcg gcgctgtccg tcgggatgct ggcgggcatg gatttgatgt cgctgctgca
     5161 cacaatgaaa gcgggcttcg gcaacacgct gggggaactg gctatcatcg tggtgttcgg
     5221 tgcggtcatc ggtaaattga tggtcgactc cggcgcggcg caccagatag cgcatacgct
     5281 gctggcgcgt ctcggtctgc gctatgtaca gctgtcggta attatcatcg gcctgatttt
     5341 cggtctggcg atgttctatg aagtggcctt tatcatgtta gcgccgctgg ttattgtcat
     5401 tgccgccgaa gctaaaatcc cgtttctgaa actggcgatc ccggcagtag cagctgccac
     5461 taccgcacat tcactgttcc caccgcagcc gggtccggtg gcgctggtga atgcttatgg
     5521 cgcggatatg gggatggttt atatctatgg cgtactggtg acgatcccaa gtgtcatctg
     5581 cgcaggtctg atcctgccga agttcctcgg caatcttgag cgcccaacgc catcattcct
     5641 gaaagcagat caaccggtag atatgaataa tctgccctct ttcggcgttt cgattctggt
     5701 gccgctgatc ccggcgatca ttatgatctc taccaccatc gccaatatct ggctggtaaa
     5761 agatacccct gcctgggaag tggttaactt tatcggttcc tcgccgattg caatgtttat
     5821 tgcgatggtg gttgcattcg tactcttcgg caccgcgcgc gggcacgata tgcagtgggt
     5881 gatgaacgct tttgaaagcg cggtgaagag cattgcaatg gtgattctga tcatcggtgc
     5941 gggtggcgtg ctgaagcaga ccatcatcga caccggcatt ggtgacacca tcggcatgtt
     6001 gatgtcccac ggcaatatct cgccctacat catggcatgg ctgatcaccg tactgattcg
     6061 tctggcgacg ggtcagggtg tggtttcagc gatgaccgcc gccgggatta tcagtgctgc
     6121 aatccttgat acagcaactg gtcagctggt tggcgtgaat ccggcgctgc tggtgctagc
     6181 gacggctgcg ggttccaaca ccctcaccca cattaatgat gcctcattct ggctgttcaa
     6241 aggttacttt gacctgtcag taaaagacac gttgaaaacc tggggtcagc tggagctggt
     6301 caactccgtg gttgggctga ttattgtgtt gattatcagc atggtagcgt aaaagagcac
     6361 cagagcctgc cattggcagg ctctggtcgg attgtcggca aagtgcgctt tgtttattct
     6421 tatctggcct acaaagggct aacgtgcggg tttgtagata gcgtagcgca tcaggcaata
     6481 ttgcgtacct gcattagcaa tgtcctgtga tttctttatt gataaacaaa agtcacgcca
     6541 ataatcgatt gcacattccc tgcagtcacc tgccctccag tacgtgcgta atttgccgtt
     6601 aatcccagac ttaccgccga agtccctact gctcctaacg ataccgtgtt attcgctgga
     6661 ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga aaacgaggcg
     6721 gtattggtga aaatcgaatt gcccgcatct gcggttgtgc cggagaggta atatcccagg
     6781 ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg gtagtccggc
     6841 agagtaacgg tgacatcacg agcagaaaca tcgcagccgc cagtgggcac caccacatca
     6901 ttattggcgt aaatattcca cacaaactga aaatcatcgc tgttatagtt gttggtctgt
     6961 cgcaaaataa gcacggcaat taatgaacca gctttaatcg ccaccccgcc agcactgctc
     7021 acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacc
     7081 cgcggcgttt cgctggtggt aggaaatgga tagctactgc cactatattt tacggtcccg
     7141 gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac atagtctgta
     7201 atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatccac gaccaggttt
     7261 tgccccacat tcacggcagg cgcaaggttt acataaacat tagcgctgcc accgccaata
     7321 gggatagcgg tgccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc
     7381 atcagcagta cagcaaacag ggtaataact cgtttcatta caatcatctc ttcgggttca
     7441 gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt
     7501 taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg
     7561 aactgttttg gttgcgccac tattcaatgt gttgccactg tcatcctgta gctctaactg
     7621 gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg ccccgctgaa
     7681 gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg
     7741 ccaggccgat gccgccccgg cagacataag actgaaagaa taaagatcgc cgagatctac
     7801 cgtggcattg gtggtggaaa ccgtgcacgg tttggcgacg accttaccgt tcaccgtgat
     7861 ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aacattgccg ccaataaata
     7921 cccacgtttg cgccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc
     7981 cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa
     8041 attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaagcgcgg acgatggggc
     8101 attaaggggt atctgatttt gttgctcatt cagaagctgt attcccagtc ccgcagccgc
     8161 tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa caccggtaaa
     8221 cccaactttt acggcagaaa cggcattacc acagggtgac agcaaaatac gaaatggaac
     8281 gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac
     8341 agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat agccgcggat
     8401 agtaatcgtg ctatccgcag ccaaagcgcg actcaccgcc aaccacaaaa aagcgcacag
     8461 aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt
     8521 taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt gagcattttc
     8581 ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga ggtaaacctg
     8641 accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca tcgccccaaa
     8701 cggcagcggc ttattattgt gggttagcgt catgagcagt tttatcccaa cgcgcgcttt
     8761 aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg cgttatctaa
     8821 atcgacgtta tcagccaggg tattggtatc cagcgctact ctgttttccc gatattcagt
     8881 ggcataaggc agcacggcat aaccgcgcca gtcggtacgc acccccgtct ggttttcgac
     8941 ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta acggctgccc
     9001 cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat agagctgctt
     9061 aatatcatcg ctatggctgt aaccgatgtt ggcattgccg taaccaccgc gataattcag
     9121 cgtggcgtag cctgtacttc cgctattacc atcacctccc ccggcgtaac cggtttgcac
     9181 gctatagctg aggttgttgt cttccagcaa cgtaccgtat acaccggcca gattggtcat
     9241 ccgaccgttg agatcgtgtg acatgctgta actggcactg gcatgtcgcc actgagattt
     9301 actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaaggcagt atgactttc
//
LOCUS       DAZPJP010000158         8858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPJP010000158 DAZPJP010000000
VERSION     DAZPJP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.159"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..818
                     /gene="gadX"
                     /locus_tag="ACHU9J_003317"
     CDS             <1..818
                     /gene="gadX"
                     /locus_tag="ACHU9J_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1906782.1"
                     /translation="LHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKC
                     VENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKN
                     LRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLAR
                     IASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSV
                     SYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1186..1914
                     /gene="gadW"
                     /locus_tag="ACHU9J_003318"
     CDS             1186..1914
                     /gene="gadW"
                     /locus_tag="ACHU9J_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1906783.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHU9J_003319"
     CDS             complement(2277..5390)
                     /gene="mdtF"
                     /locus_tag="ACHU9J_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1906784.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHU9J_003320"
     CDS             complement(5415..6524)
                     /gene="mdtE"
                     /locus_tag="ACHU9J_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1906785.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6632..6910
                     /locus_tag="ACHU9J_003321"
     CDS             6632..6910
                     /locus_tag="ACHU9J_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906786.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6911..7438)
                     /gene="gadE"
                     /locus_tag="ACHU9J_003322"
     CDS             complement(6911..7438)
                     /gene="gadE"
                     /locus_tag="ACHU9J_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1906787.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8237..8809)
                     /gene="hdeD"
                     /locus_tag="ACHU9J_003323"
     CDS             complement(8237..8809)
                     /gene="hdeD"
                     /locus_tag="ACHU9J_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1906788.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2378 a         2065 c         2065 g         2350 t
ORIGIN      
        1 cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc accatggtta
       61 atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc
      121 gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc
      181 tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac
      241 tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga
      301 ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat
      361 ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca
      421 tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc
      481 gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
      541 agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg
      601 ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt
      661 atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat
      721 cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt
      781 acggcactga ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa
      841 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
      901 taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata taacttttac
      961 tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg aaaatatatt
     1021 cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt ggataaattt
     1081 tggtcctacc aaatctggca gttttttcgc taagaaacag tctggcaaca tttcattagt
     1141 atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac tcatgtctgc
     1201 tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcataa aatatcgctg
     1261 cataacgaga gtatcgtgct gctggagaaa aatttggcag acgattttgc gttttgttca
     1321 ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt acaaaatatt
     1381 cgtcagctac cacgcaattt agggttacat agcaaagacc gtctattaat taaccagtca
     1441 ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg ggtaaattca
     1501 ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca taagaaagaa
     1561 ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga acgccttatt
     1621 agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat gtataccagc
     1681 gagagtctca taaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa aatattactc
     1741 gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc tctgcatact
     1801 attgcagaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt tcgacaatat
     1861 tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc ctgacatatt
     1921 ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct tccctgatga
     1981 tttctgccgg gctaccggct agttctcttt cgcagtttgc caggcggtat aggtattttc
     2041 aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac gattacggga
     2101 atgtgggtca gtattgtgct gttcaaattg atgacgaatg gctggagtgg tttctacttt
     2161 cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag tggtgaatgg
     2221 ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt tacacgttac
     2281 gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg aacgaagtaa
     2341 attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac cgcgttttgc
     2401 gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat aaaggcgaga
     2461 gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc ctctatcggc
     2521 gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat ggcgttcttg
     2581 gcggaaagcc cgatggtggt cagcaaacca acctggaagt agacgtcatt acttaagccg
     2641 cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa catcaccgag
     2701 aacggaattg accagctctc atagagtgcg gcgagggcga ggaacaccac gaccagtgaa
     2761 atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga cagtccggtc
     2821 catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa tttcatagcg
     2881 tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg gatgccgttg
     2941 tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc agaaagcggt
     3001 gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg caacatacgg
     3061 aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag gaagtcgttc
     3121 acgtagctgc tgccgaaggc ggtggaaatt gtctggttga tatcagacag cgccacaccc
     3181 attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc ttccaggccg
     3241 ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa cagctcgttt
     3301 cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc catatcaaaa
     3361 cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac gacggcttta
     3421 ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga gttttcctca
     3481 ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt ttgcccctgg
     3541 ccgctgaagc caaagccgcc aacggtaaac accgactgaa cattatcttt ctcttttgta
     3601 agataataat ccgtcacctg ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa
     3661 ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa agaggtcggt
     3721 gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac catgtagcga
     3781 ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga tttttcaaac
     3841 agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg cgcggctttc
     3901 agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga aagcagcatg
     3961 gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcgcc gctcataaag
     4021 gccatcggca taaacactgc ggaaagaaca acagcaatac cgaccagcgc acgttggatc
     4081 tgccccatcg atttatgcgt cgcttccttc ggtggtagct tatcttccgc aatgacacgc
     4141 tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc cagcaccatc
     4201 ccgaacatag ttaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg
     4261 agaataacca ccggtacggc aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac
     4321 agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttaaa aacttcctga
     4381 atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg
     4441 aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc cagggcgttt
     4501 gctccggtag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg
     4561 gtggaataat cttccgctcc aagttcgacg cgggcgacat cacgcagcag cacttgcgaa
     4621 ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg cagacgcgtc
     4681 tgcacaatga tcgaggcgtt tagttgctgg tctgccgcct gtggcatgcc acccagttga
     4741 ccaccggaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga aggtaccaga
     4801 ttgtatttat tgagtttttg cgggtccagc cagatacgca tggcgtactc ggaaccaaag
     4861 agctgtacgc taccaacgcc cgcggtacgg cttagcgggt ctttgatatt agacgctaca
     4921 tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc cgctaccatc
     4981 agaatattac tgctcgactt atcaacgcta atcccctgct gctgcaccgc ttcaggtaat
     5041 gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc aggagatgtc
     5101 ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt tgaagacatg
     5161 tacatcaggc catcaagccc attcatattt tgctcaatca cctgagtgac cgagtcttct
     5221 accgtttgcg catcggcacc tggataggta gcgctgacgg taatggtcgg tggcgcaatc
     5281 tgcggatact gcgcaaccgg taagttcatg atcgccagac cacctgcaag catcataata
     5341 atggcaagta cccaggcaaa aaccgggcga tcaataaaat agttagccat gaaagtcccc
     5401 ttaagcctgc aacgttattg tttcgattcg gtgctggcgt tttcctggct ggaggaaatt
     5461 gctcgtgctt tgatacccgg acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca
     5521 gcctgcaagc cagaggtgac aacccactgg tcgccgatgg ctttgctggc ttcaatttcg
     5581 cgtagctgca caacatcgtc tttatccaga atgagcgccg ttgctttacc ctgggcgttg
     5641 tgagtgacgc cttcctgcgg caccagtaat acattctggc ggctaccttc atccactaat
     5701 gccgtgacgt acatgccagg cagcaagtca ccatttgggt tggggaaaat cgcccgtaac
     5761 gtcacggagc ccgtggtttc atccaccgtc gggtcggaga atttcagtgt gccggtctgg
     5821 ctgtagcgtt taccattttc cagattgagc tgtactggcg tactgccctg aacctgtttg
     5881 atttgcccac tggcgacctc ttctttcatg cgcaagaaat cctgcaccga ctgcgtgaga
     5941 tcgacataaa tcgggtccag acgttgtacg gtaaccagcg aatctgcctg attagcggta
     6001 acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca
     6061 ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg
     6121 gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat cctgacggct aacgtagttg
     6181 gtcttcagca acgatgcctg gcggttaaag gtgatgcggg cattgctggc ggtagagagc
     6241 gctttcgcca gcgagccttt ggcggagttt agctcggcct gtaaaggtgc aggatcaatc
     6301 tgatacagcg aatcgccctg gttcacttta tcgccttcga taaagttgcg tttaatgata
     6361 ataccgccca cctggggacg tatctcggca acttcataag gaacggttct accgggcaat
     6421 tcgctcaaca cattgaccga accgggggag agtgtgacga caccgacctc aggcgtcatg
     6481 gcggcggcgt tttccgccga tttgtcatcg caggcggtga gcatcgcgcc gcagaataac
     6541 aacggtatta acagctttct tcttctgttc attttagtcc ctgaaaattc ttgagaaata
     6601 acggttcttt aaattgcgcg cgaggagaga tatgagggat ttacagacca gcggcatagt
     6661 ggggttgtca gccagtaagg tgggatacag gcacagtgat cgacatggtg aggtcaacga
     6721 caaaaaaaat gttgccgttc tgccaacagt tcctgccagc attcgggcaa cgaagggttc
     6781 tactggtgga tacacatacc tggggaataa agtcgtttta gcctctcctt tagtaatcac
     6841 cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc agccatcaat ttcagttgct
     6901 tatgtcctga ctaaaaataa gatgtgatac ccagggtgac gatgtcgctc atacgtctta
     6961 gtttcagctt gtacataatg ttgaatttct gaatctttaa tgtttgttgc gaaatattga
     7021 gcgtcgacgt gatattgctt tcatttttat ttttcagaat cagttgcata attttgaact
     7081 gattatgtga aaaacataat tgtgaattct tatggggcaa gtgtttacca taagtaatct
     7141 caagaagatt tttgatactt tctttgcggc taacaaaata aataggcgct ttagctttta
     7201 gtttactgat gtgataaggt gccaaaacaa taattctctc ggcatctaat ttctccagaa
     7261 atttaatcgc ttcttcatca aggatatgat tgtgataggt atcaatgata accttgaagg
     7321 gtttattgcc tacttttttg atctctgaca gggaattgat ttttatcatt tcgtgattat
     7381 ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa
     7441 cttgctcctt agccgttatc gtttcgaaca tgtcatcctt ttcattattt acatccttgt
     7501 ccgaatcgtt gttcaatata gtaaacgcct ataagaactt tacccttcgt gaactaatac
     7561 gtctattaac actaatcaat acttcagatt tatccatcac gcactccata tattgatcct
     7621 aagtaagatt cctggttgtt atcagcttgt aaatagtaaa caaaaagtta acgtttgcgg
     7681 attactgccc aagaataagt tatcaataac cagtttgtga tctctgaaga atattactaa
     7741 agttaaaatg taatccgatt taaatatcga gtctccttgt ttcgacttaa gctggcaatt
     7801 ggattgccag ctttctttta acgcattgaa attacctgag taaataacgc attcatgtct
     7861 tcaggttttt atatagcgtc tggagacacg gatatttatg caatgtaatc aacgccagtt
     7921 gcaaaaaaat gaacccggat tacttattta accgcccata gcatcaacgc ttacggaact
     7981 ctttgttcac tttcccacag cacttttaaa acattaaacc tgactacgga aaatatcagc
     8041 catgataatg tttgaaatgg ctaatttgcc atggagtgaa aaaaattaga tgaaattcag
     8101 taggttgaaa taatcactag caggtaatta tttcaatgat agtgcgcaat tgatctacaa
     8161 cactccgtag cggagagagt attaatcgga tcatagtcat atcaagtgac tatgatccgg
     8221 gtgacaaccg ggataattat tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg
     8281 cggcgctaaa tatcagttca attcccacca gtgtggaaac cagcgttaca gacaccatcg
     8341 gcgttgcacc aaggaatatc caggcaatga cgatatccag cacaccaata acgagctgta
     8401 gccagctgcc tttcattgaa cgctgacgat accaactcat caggcgaata acccctgcaa
     8461 cacagaacaa accggcaata aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga
     8521 tgaagaaata gccgatcaat aaataggcga ctgcgacgag gaaaccggat aataccggcc
     8581 agaaattatg actgcggttg ctgaataacc cgacaataag cgcaatgccc gagcagatta
     8641 ataatgcacc cactactgtg cttaaaatat cgccagagac gaacgggaaa ctgatacaca
     8701 gcaacccgac gataaacagt agcacggcaa taaactggat tgctctgcga tgttttttaa
     8761 gcatctccag atcaaacttc aaaattgttg ccttatctat atataacata gaaccaccct
     8821 ataaaattaa gaagaaaatc ccctgctatc aatctatg
//
LOCUS       DAZPJP010000159         8857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPJP010000159 DAZPJP010000000
VERSION     DAZPJP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.160"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            137..1535
                     /gene="ulaA"
                     /locus_tag="ACHU9J_003324"
                     /pseudo
     CDS             137..1535
                     /gene="ulaA"
                     /locus_tag="ACHU9J_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1551..1856
                     /gene="ulaB"
                     /locus_tag="ACHU9J_003325"
     CDS             1551..1856
                     /gene="ulaB"
                     /locus_tag="ACHU9J_003325"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1906789.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1866..2327
                     /gene="ulaC"
                     /locus_tag="ACHU9J_003326"
     CDS             1866..2327
                     /gene="ulaC"
                     /locus_tag="ACHU9J_003326"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1906790.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2341..2991
                     /gene="ulaD"
                     /locus_tag="ACHU9J_003327"
     CDS             2341..2991
                     /gene="ulaD"
                     /locus_tag="ACHU9J_003327"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1906791.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3001..3855
                     /gene="ulaE"
                     /locus_tag="ACHU9J_003328"
     CDS             3001..3855
                     /gene="ulaE"
                     /locus_tag="ACHU9J_003328"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1906792.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3855..4541
                     /gene="ulaF"
                     /locus_tag="ACHU9J_003329"
     CDS             3855..4541
                     /gene="ulaF"
                     /locus_tag="ACHU9J_003329"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1906793.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4638..5189
                     /locus_tag="ACHU9J_003330"
     CDS             4638..5189
                     /locus_tag="ACHU9J_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1906794.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5264..5539)
                     /gene="yjfY"
                     /locus_tag="ACHU9J_003331"
     CDS             complement(5264..5539)
                     /gene="yjfY"
                     /locus_tag="ACHU9J_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1906795.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5866..6261
                     /gene="rpsF"
                     /locus_tag="ACHU9J_003332"
     CDS             5866..6261
                     /gene="rpsF"
                     /locus_tag="ACHU9J_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1906796.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6268..6582
                     /gene="priB"
                     /locus_tag="ACHU9J_003333"
     CDS             6268..6582
                     /gene="priB"
                     /locus_tag="ACHU9J_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1906797.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6587..6814
                     /gene="rpsR"
                     /locus_tag="ACHU9J_003334"
     CDS             6587..6814
                     /gene="rpsR"
                     /locus_tag="ACHU9J_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1906798.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6856..7305
                     /gene="rplI"
                     /locus_tag="ACHU9J_003335"
     CDS             6856..7305
                     /gene="rplI"
                     /locus_tag="ACHU9J_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1906799.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7417..8388)
                     /locus_tag="ACHU9J_003336"
     CDS             complement(7417..8388)
                     /locus_tag="ACHU9J_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1906800.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8441..>8857)
                     /locus_tag="ACHU9J_003337"
     CDS             complement(8441..>8857)
                     /locus_tag="ACHU9J_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1906801.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2134 a         2164 c         2355 g         2204 t
ORIGIN      
        1 ccgtgtggaa ttatttgcgg gtcgcgtcac atttaatcat aaataatctt gttgtgatta
       61 cttttgaaaa ttagagtgag tgcacaacat tccgggtgtg cggaataccc ggttacctct
      121 tcttcaggag atcgttatgg agatcctcta caacatcttt accgtgtttt ttaaccaggt
      181 catgaccaat gccccgttgt tgctgggtat tgtgacctgt ctgggctaca tcctactgcg
      241 caaaagtgtc agcgttatta ttaaaggcac gattaaaacc ataattggtt tcatgttgtt
      301 gcaggcaggg tccggcatcc tcaccagcac cttcaaaccg gtggtggcga aaatgtccga
      361 agtctacggc attaaacggc gcaatttccg atacttacgc gtcgatgatg gcaaccatcg
      421 accgcatggg cgatgcctat agctgggtag gttacgcggt gttgttagcg ctggcgctga
      481 acatctgtta cgtgctgctg cgtcgcatta ccggtattcg taccatcatg ctgactggtc
      541 atatcatgtt ccagcaggcg gggctgattg ccgttacgtt gtttattttc ggctactcca
      601 tgtggacaac cattatctgc acggcgattc tggtttcgct ctactggggc attacctcca
      661 acatgatgta caagccgact caggaagtga cggacggctg cggtttctcc atcggtcacc
      721 agcaacagtt tgcatcatgg actgcctata aagttgcgcc gttcctcggc aaaaaagagg
      781 aaagcgttga agacctcaaa ctgccaggct ggctgaatat tttccacgac aacatcgtct
      841 ccacggcgat tgtcatgacc atcttctttg gtgccattct gctctccttc ggtatcgaca
      901 ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta tatcctgcaa accggttttt
      961 ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg catgtttgtg gcggaactct
     1021 ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg tgcggttctg gcgattgact
     1081 gtgcggctat ttatagcttc gcgccgaacg ccgtggtctg gggctttatg tggggcacca
     1141 tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg ttcctcgatc ctgattatcc
     1201 caggctttat cccgatgttc ttctccaacg ccaccatcgg cgtgttcgct aaccacttcg
     1261 gcggctggcg tgcggcgctg aagatttgtc tggtgatggg gatgatcgaa atctttggtt
     1321 gcgtctgggc ggtgaaactt accggtatga gtgcctggat ggggatggcg gactggtcga
     1381 ttctggcacc gccgatgatg cagggcttct tctccatcgg tatcgccttt atggccgtca
     1441 tcattgtaat tgcactggct tatatgttct tcgctggccg cgcactgcgc gcagaagaag
     1501 atgcagaaaa acaactggca gaacagtctg cttaataagg agttttgatt atgaccgtac
     1561 gtattctggc tgtgtgtggc aacggacaag gcagttccat gatcatgagg atgaaagtgg
     1621 accagttttt aacccaatca aacattgacc acacggtaaa tagctgcgcg gttggcgaat
     1681 acaaaagcga gttgagcggc gcggatatca tcatcgcttc tacgcacatt gcgggcgaaa
     1741 tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa catgctctct cctgccgact
     1801 ttggcccgaa actgctggaa gtgatcaaag cgcatttccc gcaggatgtg aagtaaggac
     1861 gcgccatgaa attacgtgat tcgctggcgg agaataaatc catccgcctg caggctgaag
     1921 ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct gctggtggcg gatgttgtcg
     1981 agccgcgtta ctaccaggcg attctggatg cggttgaaca gcacggtccg tactttgtgc
     2041 tcgcaccagg cctggcaatg ccgcacggtc gcccggaaga gggcgttaag aaaaccggtt
     2101 ttgcgctggt gacgctgaaa aagccgctgg agttcaacca cgaagataac gacccggtgg
     2161 atatcctcat caccatggcg gcggtcgatg ccaacacgca ccaggaagtg ggcatcatgc
     2221 agatcgtcaa cctgtttgaa gatgaagaaa atttcgaccg tttacgtgcc tgccgtaccg
     2281 agcaggaagt actggatctc attgaccgca ccaacgcggc agcttaagaa ggaattgaag
     2341 atgtcattac cgatgttgca agtcgcgctg gacaaccaga ctatggatag cgcctacgaa
     2401 accactcgcc tgattgccga agaagtcgac attatcgaag tgggtaccat tctgtgcgtg
     2461 ggcgaaggcg tgcgtgcggt tcgcgatctg aaagcgctct acccgcacaa aatcgtgctg
     2521 gcagacgcca aaattgccga tgcgggcaaa attctttctc gtatgtgctt cgaagccaac
     2581 gctgactggg tgaccgtgat ttgttgtgcg gatatcaaca ccgccaaagg tgcgctggac
     2641 gtagcaaaag agtttaacgg cgacgtgcag atcgaactga ccggttactg gacctgggaa
     2701 caggcgcaac agtggcgcga cgcaggtatt cagcaggtgg tttatcaccg cagccgtgac
     2761 gcgcaggccg ctggcgtggc gtggggtgaa gccgatatca ctgcgatcaa acgtctttcc
     2821 gatatgggct tcaaagtcac cgtcaccggt ggcctggcgc tggaagatct gccgctgttc
     2881 aaaggcattc caattcatgt gtttatcgcc ggtcgcagca tccgtgatgc cgcttctccg
     2941 gttgaagccg cgcgtcagtt caaacgttcc atcgccgaac tgtggggcta aggagcggat
     3001 atgttgtcca aacaaatccc gcttggcatc tatgaaaaag cgctccccgc cggggagtgc
     3061 tggctggaac gcctgcgact ggcaaaaacg ttgggcttcg attttgtcga gatgtcggta
     3121 gatgaaactg acgagcgcct gtcgcgcctc gactggagcc gcgagcagcg tctggcgctg
     3181 gtgaatgcga ttgttgaaac cggcgtgcgt gtgccgtcca tgtgcctttc tgcccatcgt
     3241 cgtttcccgt tgggcagcga agatgacgcg gtgcgggcgc aggggctgga gattatgcgt
     3301 aaagccatcc agttcgccca ggacgttggc attcgcgtga tccagctggc gggctatgac
     3361 gtttactatc aggaagccaa taacgaaacg cgtcgtcgtt tccgtgacgg cctgaaagag
     3421 agcgttgaga tggcgagccg cgcgcaggtg acgctggcga tggagatcat ggattatccg
     3481 ttgatgaact ccatcagcaa ggcgctgggt tacgcgcact atctcaacaa cccgtggttc
     3541 cagctctacc cggatatcgg caacctgtcg gcgtgggaca acgatgtgca gatggagttg
     3601 caggccggaa tcgggcatat cgtcgcggtg catgtgaaag acaccaaacc gggcgtcttc
     3661 aaaaacgtgc catttggcga aggtgtggtg gatttcgaac gttgcttcga aacgctcaaa
     3721 cagagtggct attgcgggcc gtacctgatt gagatgtgga gcgaaacggc ggaagacccg
     3781 gcggcagaag tggtgaaagc gcgtgattgg gtgaaagcgc gcatggccaa agcaggcacg
     3841 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
     3901 cgcgctatgg gctggtgacc tttacctggg gcaacgtcag cgctatcgac cgcgaacgcg
     3961 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
     4021 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccgtct tccgacaccg
     4081 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
     4141 ccactcatgc tactgcatgg gcgcaggcgg ggctggcgat cccggcgtta ggcactacgc
     4201 acgccgacta cttctttggc gacatcccgt gtacgcgcgg attaagcgaa gaagaggtgc
     4261 agggcgagta tgaactgaac accggcaaag tgattatcga aacgctgggt gacgccgagc
     4321 cgctgcatac gccggggatt gtggtgtatc agcacggtcc gttcgcctgg gggaaagatg
     4381 cccacgacgc ggtgcataac gcggtggtga tggaagaagt ggcgaaaatg gcgtggattg
     4441 cgcgcagtat taacccacaa ctgaatcaca tcgacagctt cctcatgaat aaacacttca
     4501 tgcgtaagca cggtcctaac gcctattacg ggcagaagta agcagtgcgc cccggaatta
     4561 ttacctcctg cgggatgtaa ttggtctgat tccggggccg cttcagttaa aaaatcgtat
     4621 aaacaacagg tgtttttatg gctatcaatg gattgtttgt cagaacggat ccgcaacgtc
     4681 gccgctatgg cgttgcgtta cttgtgggtg tactttcagg aattatttca gcatttgtta
     4741 aatggggagc ggaagtcccc ttgccaccgc gcacattttc cggtggtcgc gatgaattta
     4801 acccgccgta tatctttcta cgggattatt taggtatcga cccaacccaa actgtgtata
     4861 cattctcgga acatattatc aacccggtga tggtgacgca tatcatcttc tcactggttt
     4921 ttgcgatagg gtattgtatt gtcgcggaaa ttttcccgaa agtgaaatta tggcagggga
     4981 tattagcagg gcttattgtg acagtggcag tccacggtat attttgcccg gcgctgaatt
     5041 taacgccgcc gctcactcaa ttgccttttg acgaatatgc ctctgaaatc ctggggcata
     5101 ttttctggtt ctggataata gaaattatgc gccgcgactt acgtaatcgc attactcatg
     5161 agccagatcc ggaagtcgca atccggtgaa taaccgcata aaaaagcccc ctgcgaaggg
     5221 ggcaaagcaa actatggcaa tgtttcgttg gttatacctg gtgctagcga taaatatccg
     5281 cgctggcgtg catattgccg ttactccccg gttcccgcat cagaattacg tggtagtacg
     5341 ttgcgccctg cgcatctgtc tcttcattta atgcctgacg cgcttcgctt tcagtggcga
     5401 aattatgatt gatataaatc acgcctaagc tttgcacatc atccatgttt ctggcctggt
     5461 ggttattaat ttcaatggct gcccatgtat ttgcacttag caaaagcaca gccagaaggg
     5521 ctaaaactcg actgaacata gatacctcct cgacggctga ctttgtgtgc tctcctctgt
     5581 gatgatcttc tgatttaatt ttaatcaatg ataaagaagt tgatggtgac catttctgat
     5641 gcagttgttc aaaaaaacac catgatgaag tgtgatgaac tccaaatcag cggtttagag
     5701 gttaattgcg aaaggggaga tttatttcgg ctctgccctt gagtttagcg gggcatacaa
     5761 gtactataac gcgtcatttt tcagccgacc tttaacacgt tccttgcctc cccgggattc
     5821 ggctgaccca gacaggaggc tgaataatcc gtaaggagca attcgatgcg tcattacgaa
     5881 atcgttttta tggtccatcc tgaccagagc gaacaggttc cgggcatgat cgagcgctac
     5941 actgctgcca tcactggtgc agaaggcaag atccaccgtc tggaagactg gggccgccgt
     6001 cagctggctt acccgatcaa caaactgcac aaagcacact acgttctgat gaacgttgaa
     6061 gctccgcagg aagtgatcga tgagctggaa actaccttcc gcttcaacga tgccgttatc
     6121 cgcagcatgg ttatgcgtac caagcacgct gttaccgaag catctccgat ggttaaagcg
     6181 aaagacgagc gccgtgagcg tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa
     6241 gctggggatt ctgaagagta atttctgatg accaaccgtc tggtgttgtc cggcaccgtg
     6301 tgcaggactc cccttcgaaa ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt
     6361 gagcatcgtt ctgtgcagga ggaagccggt tttcaccggc aggcgtggtg ccaaatgccc
     6421 gttattgtta gcggacacga aaaccaggcc attactcaca gtataacggt cggcagtcgc
     6481 ataaccgttc aggggttcat ttcatgccac aaggcaaaga acggactgag caaaatggtt
     6541 ttgcatgccg agcagattga attgatagat tctggagact agccatatgg cacgttattt
     6601 ccgtcgtcgc aagttctgcc gtttcaccgc ggaaggcgtt caagagatcg actataaaga
     6661 tatcgctacg ctgaaaaact acatcaccga aagcggtaag attgtcccaa gccgtatcac
     6721 cggtacccgt gcaaaatacc agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct
     6781 gtccctgctg ccgtacactg atcgccatca gtaatcggtc acggtccatt aatacgactt
     6841 tgagaggata aggtaatgca agttattctg cttgataaag tagcaaacct gggtagcctg
     6901 ggtgatcagg taaacgttaa agcgggctat gctcgtaact tcctggtacc gcagggtaaa
     6961 gctgttccag ctaccaagaa aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa
     7021 gctaaactgg ctgaagttct ggcagctgct aatgctcgcg ctgagaaaat caatgcactg
     7081 gaaactgtta ccatcgcgtc taaagctggc gacgaaggta aactgttcgg ttccatcggt
     7141 actcgcgaca tcgctgacgc tgtaactgca gctggcgttg aagtggctaa gagcgaagtt
     7201 cgtctgccga acggcgttct gcgtaccact ggcgagcacg aagtgagctt ccaggttcac
     7261 agcgaagtat tcgcgaaagt gatcgtaaac gtagtagctg aataattcgt tattcaacga
     7321 gacgtaaaaa cgccgaccag tggtcggcgt tttgcttttg tagcgagcac ttgcagatat
     7381 cgataagggc cacaataagc agccctacga agataattac ttagctgcct gaatatcagc
     7441 cagcattggg gcaagggctg ggttgtcctt agcgaagttt gcgtacattg gctgtacttt
     7501 ctcgataaac ggagctttat ctacctggta gactttcacg cccattttat ccagttttgc
     7561 aaattcttgt ttctcttcct cgctccacaa gtctttcatg tagctcattg attctttagc
     7621 tgcttttaat ataactgcct gattttccgg gctaagttta tcaaacgcag cattgctcat
     7681 cagaaccaca tcgggaacca tagtgtgttc gtcaaggctg aaggattttg ccacttcacc
     7741 gtgacgcatg gtggtcagcg ccatcacgct gttttctgcc atatcgacca caccctgctg
     7801 taaggctgta tacaactcgc cgtaatccag tggcgtcggg ttaccgccca tgactttgat
     7861 catttcaact gcgccagggc ttggctggac acggactttc atgcctttca gatcgtctgg
     7921 tttcagtact ggtttattgc catagaaact gcgggcgcct ccatcataaa aagtcagccc
     7981 gaaataacct ttgcttttcg ttgagtcgag gatttttctc ccgatatcgc cctgcatgac
     8041 ctgataataa tgatcacgat cgcggaacaa ataaggcagg ctaaataatt tataggaatt
     8101 ttcaaatgct tccatttctg ccgcattggt ttttaccagt ggaatagcgc cagaacgaat
     8161 cagctccatt gtttctcgtt gagtacccaa tgttccattt ggataaatac gaatagtaat
     8221 atcaccgtta gagtactctt tgttcttttt cgcaaagaac tccatcgctt tatgaacagg
     8281 atgagactta tcctgattat gactcagttt taatgtcact gcccaggact ggcttgcgga
     8341 taacataaga acagcaccga taatgccagc ggatactttt attttcatag atgttccttt
     8401 tctaataaat atggcggcag gtgccgccag tgaatgcgca tcagtagccc agtgcttttg
     8461 gcaatgccat actgatttgc ggaatatagg tcaccattgc cagcaccaat accatcacgg
     8521 catagaatgg cagcataggt ttgataattt tattaatcgg taatttagat acactacaac
     8581 cgacaaataa cacactgcct acaggcggag tacaaatacc gatacacatg ttcagtacca
     8641 tgacaatacc aaagtgaatc gggtccatgc ccagttcggt gacaacaggt aaaaatatcg
     8701 gcgtaaaaat cagtaccgcc ggtgtcatat ccataaaagt gccgataatc aacaggataa
     8761 tattgataat aagcaggatt attattgggt tgtcagaaat agtgttaagc aaatcgagaa
     8821 tcaggaaggg aacgtcagcg tttgacattg cccatga
//
LOCUS       DAZPJP010000160         8836 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPJP010000160 DAZPJP010000000
VERSION     DAZPJP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.161"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHU9J_003338"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHU9J_003338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR1906802.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHU9J_003339"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHU9J_003339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1906803.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHU9J_003340"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHU9J_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1906804.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHU9J_003341"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHU9J_003341"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1906805.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2168 a         2049 c         2259 g         2360 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
     8821 aaccttgaat tattat
//
LOCUS       DAZPJP010000161         8800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPJP010000161 DAZPJP010000000
VERSION     DAZPJP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.162"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..554
                     /locus_tag="ACHU9J_003342"
     CDS             <1..554
                     /locus_tag="ACHU9J_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906806.1"
                     /translation="LYVNKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVI
                     QRITTFFHTSPNVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVL
                     DEIQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLS
                     IVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(797..1492)
                     /locus_tag="ACHU9J_003343"
     CDS             complement(797..1492)
                     /locus_tag="ACHU9J_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1906807.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1485..2912)
                     /locus_tag="ACHU9J_003344"
     CDS             complement(1485..2912)
                     /locus_tag="ACHU9J_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1906808.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2923..3642)
                     /locus_tag="ACHU9J_003345"
     CDS             complement(2923..3642)
                     /locus_tag="ACHU9J_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1906809.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHU9J_003346"
     CDS             complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHU9J_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR1906810.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5250..6575
                     /gene="rclA"
                     /locus_tag="ACHU9J_003347"
     CDS             5250..6575
                     /gene="rclA"
                     /locus_tag="ACHU9J_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR1906811.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6684..6920
                     /gene="rclB"
                     /locus_tag="ACHU9J_003348"
     CDS             6684..6920
                     /gene="rclB"
                     /locus_tag="ACHU9J_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR1906812.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6932..7525
                     /gene="rclC"
                     /locus_tag="ACHU9J_003349"
     CDS             6932..7525
                     /gene="rclC"
                     /locus_tag="ACHU9J_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1906813.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7685..8554)
                     /locus_tag="ACHU9J_003350"
     CDS             complement(7685..8554)
                     /locus_tag="ACHU9J_003350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1906814.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2433 a         2111 c         1873 g         2383 t
ORIGIN      
        1 ttctgtatgt caataaagat aatataaaag tgagttacag cctaaaaata aaccagacaa
       61 caccaggcat acttgttaac tgtgatagca ataataactt tgcctgtcag accacaatga
      121 ctgaagatgt tatacagcga attactacat tttttcacac cagcccaaat gtcaagaaca
      181 gagaaataaa gctggaatgg tcaggagata agagagattt accaactgct gaagcggaaa
      241 tatctcgcgt gcaggcgtct attatcaaat ggtatgcgtc agaataccat aatggcaggc
      301 aagttctcga tgagatacaa ataccttcag caattaacag tgagctttat acaaaaatga
      361 tatacctgac caggaactgg tcattgtatc cgaacggtga tggctgtgta actataagct
      421 caccagaaat aaaaaataaa taccctgctg ccatttgcct ggctctggga ttttttctaa
      481 gcattgtaat ttctgtaatg ttttgccttg tcaaaaaaat ggtagatgaa taccaacaaa
      541 actctgggca gtaataaaca gcattctgat agagagtgcc tgtataaagc tgaagtgaca
      601 atgaccggga agggaacccg ttttcaggaa tggatgcctg tctttttcac tgtaaatatc
      661 ctggcattac taatgccagg atatttacag aagagcagca taaccatgac ggaacggggt
      721 taagtcaggt tgtccttaac gctattttca acctcgtatg ccttcttcag gtttatgttc
      781 agatctcata tctctctcag caatcctcaa taatcagata caccgctttc accggaccat
      841 gaaccccgac gactttgata agctcaatat ccgccgttga gctgggacca ctgatgatgt
      901 taatacatga cggcattcgt tcaccggcct gcgctttctg atgcaatttt tctgcgagtt
      961 gcgctacacg cggcaggatt gtgcttttac gcaggataaa aagagaagat tccgggagca
     1021 ggctcaatga acgcccgcgc tcgacggcgg aaaaaagaac cacgcctccc gattcggtta
     1081 aaccatattc agcatacacg acacccactt tagcctgctc tgcctgcgag atattctcgg
     1141 cacctttcgc cggatcccaa acaacggcat tgcattcctg ctgcaaacgt tcactaatcc
     1201 ccaattcctc cagcctcgtg tcaccgctaa tcacgaccga ctgatctccc agctctttac
     1261 acagacgtat tgcagcttct gccgccttcg cctcgctggt cagctcacag cgcgtcaaca
     1321 taacatcgct ggcaaactga ataaacgcgt cacagcgctg ctgttggtta agttgggtaa
     1381 gccgctcgtt agcatagttg ttaagcggcg catcttctgc ttgcggttca agtcgcagcg
     1441 ggcgacccag tgcctgagca acgttattca aaaattcgct ccgattatcc attctttttc
     1501 tcctgcgcct gatgtttctt aaaccaacta cggaaactct ctccgtcagc ttcaggaaga
     1561 tcgcgtgctt ccatccagtc gctaatcgcg ccaaatttga gtggtgtttt gccgccattg
     1621 ataaaccagc ttgccgcatg agcgccggcc atcatcccga ctttccacaa tcctggatga
     1681 ctattggcat aagcgaacat ttttatcgcc cgttgctctg cttttgcggt gatccctttt
     1741 tcagccatca cccgacgatg acgcaaaatc agttttgaca gcggaatacg caccggacac
     1801 acgctgtcac aagcagtgca taaagagcag gcgtagggta aatctttaaa atctttatag
     1861 ccgccaagta gcggagaaat caccgcacca attggccctg gataaataga gccatatcca
     1921 tgaccgccaa tatggcgata tgccggacaa gtattcatac aagccccgca gcgaatacag
     1981 cgcagcacat cccgaaattc agaggccagc acctcagaac gcccgttatc gacaataacc
     2041 agatgaaact cttcaggacc atcaacgtgc cccgcttcgc gcggtcctgt cagccaggtg
     2101 ttgtatcccg tcaaacgtgc accaacggca ctgcgcgcca gcatggtgat caatacatct
     2161 acctcggcaa acgtgggggc aatacgctcc attcccatca ctgcaatatg cgttttaggc
     2221 agcgtggtac acattcgcgc attaccttca ttggtcacta ggcataccga acctgtctct
     2281 gccaccgcga aattacagcc ggtaatacct atttcagcac tgaggaaatc ttcgcggatt
     2341 ttttgccgga tgaataaggt catcgcttca ggcgtttccg gcccctcata gcccagacgt
     2401 tcgtgtagca ctcgacggat ctgatggcga tctttatgaa ttgccgggac cacaacatga
     2461 gatggcggat cttgatccag ctggagaata tattcaccca gatcggtttc aatcacctga
     2521 atgccagcat cctgcaacac atgattgaca ccaatctctt cggtcaccat cgatttagat
     2581 ttcaccacct tccgggcatt tttgcgttgg gcaacctgta aaatgtagcg ggtagcgtct
     2641 tctttggttt ttgcaaacca gacgtgaccg ccgttttgcg tcactttttc tgagagctgg
     2701 tacagataag cgtcgagatt actcagaaca tgatcacgta tctgggcggc ccgatcgcgc
     2761 cactcctccc agtgccccaa ttcatcgacc attttttgcc gatttgcccc aatacgctgc
     2821 tgcgcgtttg ccaccgcttt gcgcatgatc ggatcttcaa tttgctgacg gatgcgtgtc
     2881 ttaaaatctg tattactggt tttgatcgac atctttatat cctcagcggc tcatcaacac
     2941 ttcagcaata tgcatcactt tgactttctg cccttcccgt tgtaatcgcc cactgatgtt
     3001 cagcaggcaa ctcacgtcag caccaattaa atactcaggg cggacttcca tcaggtgcac
     3061 aaccttttct ttcaccatct cgccggatat ttcggccatt ttgaccgaga acgtgccgcc
     3121 aaatccgcag caggtatcct gttcagcaaa ggtcaacagc tccagtccac gcacattttt
     3181 cagcagcgta agtggctcgt ccttcactcc cagcttacgg gccaggctac aagatgggtg
     3241 atacacagct ctcccttgca aactggcacc tacatcgact acccctaatt tattaacaat
     3301 aaaagaggtg agatcctgca tacgcgcggc aaccttttcg gcacgtgatg cccattcggg
     3361 ttcatccgcc agatacgtcg ggtaactttt tacggcatag gtgcaagagc cagccggtga
     3421 aataatggga tcgtcgttat cctccagtgc ggcgatcaga tttttcatcc ctggaatcgc
     3481 ttctttgata taaccgctat tgatcgcagg ctgaccgcag catccctgtt tctccgggaa
     3541 atttacgcga cagccgagtt tttccagtag cagcacggag tctcgtgcca ttcttgattt
     3601 cagggcgtca ccaatacagg tgacaaagaa attgacattc acaactaata ctccattact
     3661 tcatgcccat ttatgcccga tggttcccct ccttccgggg gatcaatcat caataaatat
     3721 ggcgttcttt tttaattggc tagcaacata ccacccctca tttgtgtgac aaatgataat
     3781 ctttcatcca aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt
     3841 tatggggaaa tcgacgttca tttttggcat aagaaatcca tttaaaaagg ctacatccct
     3901 tatacctatt cagagctact tattcagggg ttattgagat atacactgat ggtgtacaaa
     3961 aaaatcaccg acaataatca cgtatcaagt aatagtcggg gttttattta attgaagcaa
     4021 tttagtttgc atttttattt aaaaactgag ctagagtcca tcagaaaaaa atatataaag
     4081 cccaaaccac cgttggaaat agaaatagtt gaaaggctca caactcccgg aatgcggatt
     4141 atctttgccc tgatttctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga
     4201 tattctcccg gagtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg
     4261 gcatagccta ctgactcagc gatcaccaca acagggagca tttcccggga aaacatctgg
     4321 gccgctattt gtagacgcaa ctttgttaat acagccagcg gcgtggttcc ggaaacatca
     4381 cggaaaagct gggcaaaact tgcccgagac atgtgggcga tgctggccag cgattcgacg
     4441 gtccaggcgt gtcctggcat ttccagcatt tgctgtatta ccgcaccaag acgtggatgc
     4501 agaagcaaac tgagaatgtt tttctctgta ttaacctgtg caatccactc acgcaccgcg
     4561 agggtaaaga atgtagcgca gatctggcta cacagtgcat ccactcccgg catcgccgat
     4621 ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc agttatattc cacgctgtgg
     4681 ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta aaaaataacg cgccgaatgt
     4741 tgcaaccgaa gagtgccgca gacaatacag gtcgattcat tatcgacatg actcaggcga
     4801 tgagctgaat tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctctccc
     4861 gtcggcattt ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aaccgataat
     4921 tccccggcac catgttgaag ctgccagtca cttcctaaca cgcaattctt atcgatcgtt
     4981 ccttgtggag cgttaagcat caacaaacgg ctaagggcat ccatatcaga ctcctgagtg
     5041 gtttttgatt acaaacacca acaatcttag taaataacta atctaatacc atcaaatggt
     5101 taatcatgat ttttgttttg tgattctgat taagaaaaag aaaataccag acgataagac
     5161 aaaatatcaa gcgttgtgaa gaaatggtat ttgctctttt gcgtctatag tcatgatgtc
     5221 aaatgaacgt gtttcgacag gaaatcatca tgaataaata tcaggcagtt attattggtt
     5281 ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa agcaggttgg cgagtggctc
     5341 tcatcgaaca atcaaatgca atgtatggcg ggacctgtat taatatcggc tgcatcccaa
     5401 ccaaaacatt ggttcatgac gcacagcagc acacagattt tgtccgtgcc atacagcgta
     5461 aaaatgaagt ggttaatttt ttacgtaata agaattttca taatcttgcg gatatgccca
     5521 atatcgacgt gatcgacggc caggcggagt ttatcaataa tcatagcctg cgtgttcatc
     5581 ggcctgaggg aaatctggaa attcatggcg agaaaatttt tattaatacc ggtgcacaaa
     5641 ccgtggttcc gccaattcct ggaattacca ccacgcctgg tgtatatgac agcaccggat
     5701 tacttaatct aaaagaattg cctgggcatt taggtatttt gggcggcgga tatattggcg
     5761 ttgagttcgc ctctatgttc gctaattttg gcagcaaggt aaccatttta gaagcagctt
     5821 cgctgttttt gcctcgggaa gatcgggata ttgctgataa tatcgcgacg attttacgcg
     5881 atcagggcgt cgatattatc ctcaatgccc atgtggagcg aatcagtcac catgaaaatc
     5941 aagtgcaagt gcatagcgag cacgcccaac tggcggtgga tgcactgtta atagcttccg
     6001 gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc cggtatcgca gtaaacgagc
     6061 gcggggcaat tgtcgttgac aagcaattac ataccaccgc agacaatatt tgggcgatgg
     6121 gagatgttac cggcgggctg caatttactt atatttcact ggatgattac cgcattgtgc
     6181 gtgatgagtt gctgggagaa ggcagacgta gtactgatga tcggaaaaat gtgccttatt
     6241 ccgtattcat gacaccgcca ctgtccaggg ttggtatgac agaagaacag gccagagaga
     6301 gtggtgctga tattcaggtg gtgacattgc ctgtagctgc aattccgcgt gccagagtga
     6361 tgaatgatac tcgtggggta ttaaaagcga ttgttgataa taaaacccaa cgtatattag
     6421 gggcatcact gctgtgtgtt gactcccacg agatgatcaa tatagtgaaa atggtgatgg
     6481 atgccgggct gccttatagc atattacgcg atcagatatt tactcatccg tcgatgagcg
     6541 aatcactcaa tgatctattt tcattagtca aataaactca aaatcagacg ccagaacaaa
     6601 tattctggcg tctcagagaa aagaatctta ttaattctct gttactctat aacttcttaa
     6661 tcacttcatt gatggtattt tatatgttta aaaaatctgt tttatttgca acactattat
     6721 ctggcgttat ggaattttcc accaatgcag atgataaaat aattctgaaa catatcagcg
     6781 tctcgtcagt atcagcatca ccgacagttc tggaggatac cattgctgat atagccagaa
     6841 aatataatgc ttcatcctgg aaagtcacat cgatgcgaat tgataataat tcaaccgcaa
     6901 cagcagtatt gtataaataa ggatgttcac aatggaaaaa tacctgcacc tgttaagtcg
     6961 gggagataaa attggcctgt cattgattcg tctgagtatt gcgattgttt ttatgtggat
     7021 tgggttatta aagtttgtcc cttacgaggc agacagcatt acaccattcg tcgcaaacag
     7081 tccactaatg tcgttctttt atgaacaccc ggaagactat aaacagtatc tgactcacga
     7141 aggcgaatac aaaccagaag caagggcatg gcaatcggcc aataatacct atggtttttc
     7201 caacggtctt ggcgtcgtgg aggtgattat tgctctgctg gttttggcta atcctgtcaa
     7261 tcgctggtta ggtttattgg gaggactgat ggcatatacc acgccgctgg taacactctc
     7321 atttttaatc accaccccgg aggcatgggt acccgcattg ggtgacgctc atcatggttt
     7381 cccttattta tccggtgctg gtcgcttggt attgaaagat actctgatgc tggcaggtgc
     7441 tgtcatgata atggcagatt cggcgcggga aattcttaaa caacgcagta atgaatccag
     7501 ttcaacgtta aaaactgaat attgataatc cgaacactgt ctgttgttca ctttttcagg
     7561 cgtcaggccc cctttctgat gatgaagcct gacgccatat taccggagat atgacagagt
     7621 gaagatcaat atcactcccc tgttaaaaga ttcttgtcgt ctcaggtaaa atatatgatt
     7681 aatatcaatc atgtattttt acagcaagac aaccacgaac aaattcagga ttaaaatgtt
     7741 ttaccgactc cccaacataa cccaaatcaa gcgaactaat ttgtgccatt tctttctctg
     7801 tcagtgagaa atcccagata gcaaaatttt cttcgatacg ctgttgatgt gtcgatttcg
     7861 gaataacgac cactccccgt tgaatattcc aacgcagaat gacttgagaa atcgattttt
     7921 gatgggcatc tgcaatactc tgaagcagat tattttcaaa gggtttatgt cgtccaccac
     7981 ctaacggagc ccatgcttca ggctgcacgt tataatactt catggttgcc agcgcctctg
     8041 gttgggcaaa atagggatgc aactcgacct ggttgaccat cggtttaact cttaccgttt
     8101 cacaaaagtt cgccagaaca tgaggataga aattggaaac cccaattgct tttaatttgc
     8161 cctcttcata ggcatcttcg agtgcacgcc acacactgaa ataatcgcgc atagcctggt
     8221 gcaataaata gaggtcgaag tactctagtc ccgatttttt taatgatgct tcaatacctg
     8281 ctgctgccgt atcctgattc aacatatcct gcccccacaa tttagacgtt ataaataact
     8341 catctcgggt acataaacct tcagatatag cttcacgaac ggcttcaccg acggcatctt
     8401 cattaccgta taccgctgca gtgtcgatga gtcgataacc cgtgcggata gcgttcaaca
     8461 ctgactgttt gcacacgttt ttatcggtaa cctgaaaaac accaaatccc atcatcggca
     8521 ttttcagatt gttacttaat acggaaaatt ccactgtcag tccctccatt aggcatgaac
     8581 aatgagtcta cgttaaaaca taacctcaaa ttagtatgtg gattttgata tcacttatgc
     8641 aaaaaattca ttaataatgt aggactgaaa cctctctatt ttcggggaca acgaagcaga
     8701 cgctaccagt gcttttgcct tcgaccttgc tgtttttgat acacttaggg ccctgggcat
     8761 aacgaaaatg tgcgatatca cgaagaatta atggagaatg
//
LOCUS       DAZPJP010000162         8719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPJP010000162 DAZPJP010000000
VERSION     DAZPJP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.163"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(310..573)
                     /gene="rpsT"
                     /locus_tag="ACHU9J_003351"
     CDS             complement(310..573)
                     /gene="rpsT"
                     /locus_tag="ACHU9J_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1906815.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            676..894
                     /locus_tag="ACHU9J_003352"
     CDS             676..894
                     /locus_tag="ACHU9J_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1906816.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            902..1843
                     /gene="ribF"
                     /locus_tag="ACHU9J_003353"
     CDS             902..1843
                     /gene="ribF"
                     /locus_tag="ACHU9J_003353"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1906817.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1886..4702
                     /gene="ileS"
                     /locus_tag="ACHU9J_003354"
     CDS             1886..4702
                     /gene="ileS"
                     /locus_tag="ACHU9J_003354"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1906818.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4702..5196
                     /gene="lspA"
                     /locus_tag="ACHU9J_003355"
     CDS             4702..5196
                     /gene="lspA"
                     /locus_tag="ACHU9J_003355"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1906819.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5321..5770
                     /gene="fkpB"
                     /locus_tag="ACHU9J_003356"
     CDS             5321..5770
                     /gene="fkpB"
                     /locus_tag="ACHU9J_003356"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1906820.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5772..6722
                     /gene="ispH"
                     /locus_tag="ACHU9J_003357"
                     /gene_synonym="lytB"
     CDS             5772..6722
                     /gene="ispH"
                     /locus_tag="ACHU9J_003357"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1906821.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6788..7702
                     /gene="rihC"
                     /locus_tag="ACHU9J_003358"
     CDS             6788..7702
                     /gene="rihC"
                     /locus_tag="ACHU9J_003358"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1906822.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7869..8690
                     /gene="dapB"
                     /locus_tag="ACHU9J_003359"
     CDS             7869..8690
                     /gene="dapB"
                     /locus_tag="ACHU9J_003359"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1906823.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1997 a         2168 c         2472 g         2082 t
ORIGIN      
        1 tgtataggaa tttaattcaa aaatagagtg gtcttaagtt taatacctca atgtgtatca
       61 caatatccac attctttgta ggggagtctg gagattgagt agatattctt gttcagaatg
      121 tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg taacggcatg
      181 tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct ctcacgcatc
      241 ataaaagcag caggcataaa aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca
      301 aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat gacgtgcagc
      361 tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt
      421 aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata ctttcttgat
      481 gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt cagactgaat
      541 ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg tgttctatat
      601 ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat ggatttgtgc
      661 aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg attctaccag
      721 cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg tcactttctt
      781 cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt actcaatcgc
      841 cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt ttgagccaga
      901 catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag ggtgtgtgct
      961 gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca
     1021 ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac ctcaaccact
     1081 ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg aaaaactgcg
     1141 ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc
     1201 ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt
     1261 tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt
     1321 acagaaagct ggcatggaat acggcttcga tatcactagt acgcaaactt tttgcgaagg
     1381 tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca atctggctct
     1441 ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc acggtgatga
     1501 attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc
     1561 ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt tacccggcgt
     1621 ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg aagtgcattt
     1681 gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat
     1741 acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga
     1801 attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt atgtaatcaa
     1861 accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga atttgccgga
     1921 aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg
     1981 gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat
     2041 tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggttaacaa
     2101 gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact cgccgtatgt
     2161 gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag aatacggtaa
     2221 gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac
     2281 ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg actggtcgca
     2341 cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc tgggcaaaat
     2401 catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg ttgactgccg
     2461 ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt ccatcgacgt
     2521 tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa
     2581 cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg cgaaccgcgc
     2641 aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc aggccgtgat
     2701 tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat
     2761 tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc cgtttatggg
     2821 cttcgacgtt ccggcaatcc tcggcgatca cgttacccta gatgccggta ccggtgccgt
     2881 tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat acggcctgga
     2941 aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc cgacgctgga
     3001 tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc
     3061 gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc
     3121 gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc
     3181 gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc aggcgcgtat
     3241 cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt
     3301 accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta ctctcgaact
     3361 aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt gggatcttga
     3421 tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg acacattgga
     3481 tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc
     3541 cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt ggttcatgtc
     3601 ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg tactgaccca
     3661 cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca ataccgtttc
     3721 gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg tggcatcaac
     3781 cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg ccgatagcta
     3841 tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc
     3901 aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg taggttgtgc
     3961 gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc acgaagtggt
     4021 acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg acatcatcaa
     4081 agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc agactgcgct
     4141 gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct tcaccgctga
     4201 tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg gtgagtggta
     4261 cgaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct gggacgagct
     4321 gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg acaagaaagt
     4381 gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt cggcgaaact
     4441 gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta ccgttgcaga
     4501 ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc
     4561 gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc aggatgtcgg
     4621 caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg
     4681 tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct acgctggctg
     4741 tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat cctccagaac
     4801 tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac
     4861 tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg gttctttgcc
     4921 ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg
     4981 cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg caacctgttc
     5041 gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg cgactggcac
     5101 ttcgccacct tcaaccttgc cgatactgcc atctgtgtcg gtgcggcact gattgtgctg
     5161 gaaggttttt tgccttctag agcgaaaaaa caataataaa ccctgccgga tgcgatgctg
     5221 acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag gcgtttacgc
     5281 cgcatccggc aaaaatcctc aaatataaga gcaaacctgc atgtctgaat ctgtacagag
     5341 caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca ccgccgagtc
     5401 tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc tttctgaagg
     5461 gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct cgttggagcc
     5521 agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc gccgtgaatt
     5581 tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa tggatggcag
     5641 tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg atttcaacca
     5701 tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg atccggcatt
     5761 ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc ggggtagacc
     5821 gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata tatgtccgtc
     5881 acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg gctatcttta
     5941 ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca cacggtgttt
     6001 ctcaggcggt acgtaacgaa gcaaaaagcc gcgatttgac ggtgtttgat gccacctgtc
     6061 cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc gaagagtcga
     6121 ttctcatcgg ccacgccggg catccggaag tggaaggtac aatgggccag tacagtaacc
     6181 cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg acggtcaaaa
     6241 acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac acgtctgatg
     6301 tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa gatgacatct
     6361 gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag gcggaagttg
     6421 tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag ctggcccagc
     6481 gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa gagtgggtga
     6541 aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt ctggtgcaaa
     6601 atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg gaaggccgtg
     6661 aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt gaagtcgatt
     6721 aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca ttttttatgg
     6781 agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg atgccgtcgc
     6841 cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca ccgtcgcggg
     6901 taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt tctggaatgc
     6961 ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc gtgatgcagc
     7021 atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca accgaaagcc
     7081 aatcgggata ccagcgtttc tggcgattcg tgatgccctg atgcgtgcac cagagcctgt
     7141 taccctggtg gccattggcc cgttaaccaa tattgcgctg ttactttcac aatgcccgga
     7201 atgtaaaccg tatattcgtc gtctggtgat catgggcggt tcttctggtc gcggcaactg
     7261 tacaccaaat gccgagttta atatcgctgc cgatccagaa gcggctgcct gtgtcttccg
     7321 tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa tattaacccc
     7381 tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgcttc atgcactgtt
     7441 tagccactac cgtagcggca gtatgcaaag cggtctgcga atgcacgatc tctgcgccat
     7501 cgcctggctg gtgcgcccgg acttattcac cctcaagccg tgttttgtcg cggtagaaac
     7561 tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc tgggcaaacc
     7621 agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt gggtggctga
     7681 ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca ttcatcgact
     7741 catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc agcgtttttg
     7801 gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat taacaaaaga
     7861 gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg gggcgtatgg
     7921 gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc gcggcgctgg
     7981 agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt gcagggaaaa
     8041 caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat gtgtttatcg
     8101 attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag catggcaaag
     8161 ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt cgtgacgccg
     8221 ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac gtcatgctta
     8281 agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa attattgaag
     8341 cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg ggagaggcga
     8401 tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt gaaggccaca
     8461 ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt gacatcgttg
     8521 gtgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc cataaggcgt
     8581 ccagccgtat gacatttgct aacggcgcgg taagatcggc tttgtggttg agtggtaagg
     8641 aaaatggtct ttttgatatg cgagatgtac ttaatctcaa taatttgtaa ccacaaaata
     8701 tttgttatgg tgcaaaaat
//
LOCUS       DAZPJP010000163         8695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPJP010000163 DAZPJP010000000
VERSION     DAZPJP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.164"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHU9J_003360"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHU9J_003360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1906824.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHU9J_003361"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHU9J_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1906825.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHU9J_003362"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHU9J_003362"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1906826.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHU9J_003363"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHU9J_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1906827.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHU9J_003364"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHU9J_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1906828.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHU9J_003365"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHU9J_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1906829.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHU9J_003366"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHU9J_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1906830.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHU9J_003367"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHU9J_003367"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1906831.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHU9J_003368"
     CDS             6614..7768
                     /locus_tag="ACHU9J_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR1906832.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHU9J_003369"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHU9J_003369"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR1906833.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2051 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagt
//
LOCUS       DAZPJP010000164         8651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPJP010000164 DAZPJP010000000
VERSION     DAZPJP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.165"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..3322
                     /gene="evgS"
                     /locus_tag="ACHU9J_003370"
     CDS             <1..3322
                     /gene="evgS"
                     /locus_tag="ACHU9J_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1906834.1"
                     /translation="RTLNIKLTIREYADHQKAMDALEEGEVDIVLSHLVASPPLNDDI
                     AATKPLIITFPALVTTLHDSMRPLTSSKPVNIARVANYPPDEVIHQSFPKATIISFTN
                     LYQALASVSAGQNDYFIGSNIITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVV
                     LNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPDLKVLEN
                     PYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLNPGGWDILP
                     GAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEM
                     YPEVEWIKVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNAS
                     LSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTT
                     LSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVI
                     SHNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDN
                     GIEKRCINHWHTLCNLPASDHAVYICGWQDITETRDLIHALEVERNKAINATVAKSQF
                     LATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYSTGQSLLGLIGEILDVDK
                     IESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALICSSTFPDHYLVKIDPQAFKQ
                     VLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYS
                     QTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEA
                     KAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDL
                     LITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTL
                     DVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAF
                     QALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVK
                     EHIAELDQEIAVFCQKNN"
     gene            complement(3378..4523)
                     /gene="yfdE"
                     /locus_tag="ACHU9J_003371"
     CDS             complement(3378..4523)
                     /gene="yfdE"
                     /locus_tag="ACHU9J_003371"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1906835.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4597..5541)
                     /gene="yfdV"
                     /locus_tag="ACHU9J_003372"
     CDS             complement(4597..5541)
                     /gene="yfdV"
                     /locus_tag="ACHU9J_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1906836.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(5611..7305)
                     /gene="oxc"
                     /locus_tag="ACHU9J_003373"
     CDS             complement(5611..7305)
                     /gene="oxc"
                     /locus_tag="ACHU9J_003373"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1906837.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(7359..8609)
                     /gene="frc"
                     /locus_tag="ACHU9J_003374"
     CDS             complement(7359..8609)
                     /gene="frc"
                     /locus_tag="ACHU9J_003374"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1906838.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         2543 a         2015 c         1781 g         2312 t
ORIGIN      
        1 aagaacgtta aatatcaaat taacaatccg ggaatacgcc gatcatcaaa aagcaatgga
       61 cgcgctggaa gaaggtgaag tcgatatagt gttatcacat ttagtagctt cgccgcctct
      121 taatgatgac attgctgcaa ccaaaccact gataattacc tttccggcgc tggtaaccac
      181 ccttcacgat tcaatgcgac cgcttacctc atcaaaacca gtaaatattg ctcgagtagc
      241 aaattatccc cctgacgagg taattcatca atcatttcca aaagcaacaa ttatctcttt
      301 tacaaattta tatcaggcat tagcatccgt ctcagccgga cagaatgatt actttattgg
      361 tagtaacatc attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt
      421 gaaatattat aactcaccgc gtcaatataa ttttttcttg accagaaaag aatctgtcgt
      481 tcttaatgaa gtactcaatc gatttgttga tgctttgaca aatgaagttc gctatgaagt
      541 atcacaaaat tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac
      601 tgaacatgaa aaacaatgga ttaagcagca tcccgattta aaggtgctgg aaaatcctta
      661 ctcccctccc tattctatga cggatgaaaa tggctcggtt cggggcgtta tgggggatat
      721 tcttaatatt attactttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa
      781 tatccatgct ggaacacagc tgaatcccgg cggatgggat atattacccg gtgctattta
      841 tagtgaagat agagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt
      901 ttttgtcatg caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc
      961 cattccatat tattatgagc ttcattcgca attaaaagag atgtatccgg aggtagagtg
     1021 gataaaagtc gataacgcca gcgctgcatt tcacaaggtc aaggaaggcg aacttgatgc
     1081 tctggtcgcg acacagttaa attcacgtta catgatcgac cattactatc ctaatgaact
     1141 ttatcatttt cttattcccg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg
     1201 agaaccggaa cttaaggata ttattaataa agcactgaat gcaatccccc caagcgaagt
     1261 tctgcgcctg accgaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct
     1321 ctatagcgag caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct
     1381 tttatgggga ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt
     1441 agaaaaccaa atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt
     1501 tgtaaactgg caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc
     1561 ggattactac aaaaatgcaa tgttaccatt agaaaacagt gactccccct ttaaagatgt
     1621 tttttctaat gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca
     1681 ggtttttgaa attgataatg gcatcgagaa aagatgcatt aatcactggc atacattatg
     1741 caatctgcca gccagcgatc atgctgttta tatttgtggt tggcaagata ttacagagac
     1801 gcgtgattta attcatgcac tcgaagtaga gagaaataaa gcgatcaatg caactgtcgc
     1861 aaaaagtcag tttctggcaa caatgagtca cgaaataaga acaccaataa gctccattat
     1921 gggcttcctg gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat
     1981 ttcacttgcc tactccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt
     2041 cgacaaaatt gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt
     2101 agtccagaac acttgtcact ctttcggtgc gattgctgca agcaaatcga tcgcattaat
     2161 ttgcagcagt acatttcctg atcattacct ggttaagatc gaccctcagg cgtttaagca
     2221 ggtcttatca aatttactga gcaatgctct caaatttacc accgaggggg cagtaaaaat
     2281 tacgacctcc ctggttcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga
     2341 ttctggaagt ggattatcgc aggaagaaca acaacaactc tttaaacgct acagccaaac
     2401 aagtgcaggt cgccagcaaa caggatctgg cttaggctta atgatctgca aagaattaat
     2461 taaaaatatg cagggcgatt tgtcattaga aagtcatcca ggcataggaa caacatttac
     2521 gatcacaatc ccggtagaaa ttagccagca agtggcgact gtcgaggcaa aagcagaaca
     2581 ccccatcaca ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag
     2641 gctattactc aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg
     2701 tgtgcaagcg ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa
     2761 tatgccgaat atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt
     2821 acccatctgg gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg
     2881 cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag
     2941 tcagttacac caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaagccct
     3001 gaaaaataat acggcgaacg atctacaact gatgcaggag attctcatga ctttccagca
     3061 tgaaacgcat aaagatctac ccgctgcgtt tcaagcacta gaagctggcg ataacagaac
     3121 tttccatcag tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt
     3181 gattaatatt agccatcagt tagaaataac acctgtttca gatgacagta agcctgaaat
     3241 tcttcagttg ctgaactctg taaaagaaca cattgcagag ctggaccagg agattgctgt
     3301 tttctgtcag aaaaataact aaatagcggc tcccacaatg ttctaatgtg ggagctattt
     3361 accagcacat cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg
     3421 agcgttgccg ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta
     3481 cccggcatca ttattcctcc ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa
     3541 ttaatggcct cagccacact taataacggc gcgacgggca caccaacttc atgtattttg
     3601 gctaaccaaa cttcagctgc ctgcgttttt aacgttcgct caatatattg tttaagaata
     3661 gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta
     3721 agctccagtg cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc
     3781 ggcttatcct gagtatcgaa aacatcaaaa ggtgccatgt agggatggcg atttcccagg
     3841 cgttgtggtg acttccctgt cgcaatatat gccatcagac catgctccag aaaactcagc
     3901 gtggcatcaa acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa
     3961 agtgcactca ctattccgct gaataaatag acaccgccgc atagatccgc aagagaggta
     4021 ccaacacgca ctggcggagc atcagggtac cctgtttcca tcattatccc actcattgcc
     4081 tgaatgatgg tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac
     4141 gatgaagcat atatgaggcg cgggttgatt tcttgtaacc tttcccatga aaaccccagt
     4201 ttttccattg tacctgggcg aaaattctca gctaatacat cagcttgttt gagcatattt
     4261 ataaatatac ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga
     4321 ttaataaaac tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca
     4381 tcaccatgac ctggtggctc aactttaatt acccttgcgc ccatattaca aagaagttga
     4441 gttccgaaag gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc
     4501 cctttgcttt cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac
     4561 acttcggcat ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata
     4621 aatccacaag ggagccgtga cgacaaagcc cagtacgctc acagccaatg acgctgtacc
     4681 agtacgggtg tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa
     4741 tgcgcctgcc agtaccatca tctgcaaatg ttcgctgttc aaatgacatg ccataccaac
     4801 gagaagcagc gccagtggca tcagaatcaa cttcaggaag gtgttataag caatttcggc
     4861 actgaactcg aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga
     4921 gttagccttc gcaatcagat taaaggtcgg gtcccatgct gccggaattt ttaccccaac
     4981 cagcaccagg atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat
     5041 taatgcgctc agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata
     5101 cagaccaata ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc
     5161 tgttgatacg gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt
     5221 aggtgaaccc gcaattaatg cacatacagc ggcttctgca tgggtacgtt taaaaaattt
     5281 gtagcaacca aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt
     5341 cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagagc
     5401 cgcaggaaga gcgtagttta ataccagttt attaaatgcg cgggcttgat cttctgaaaa
     5461 agtttctcgt ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc
     5521 gccaataaaa aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa
     5581 tgagttggca agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag
     5641 tttggtaata tggccacttt cagttcctgc agcagggtcg atgaccacat taataatggt
     5701 cggtttgcgc gactgaatac cggtggttaa agcatgacga agttcatctg tcgtggtgac
     5761 gttatagcca acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag
     5821 atccgttggt gatggtgcac cagcgccact gagatcaaca ccgtctcctc tgtagatgcc
     5881 gccattatta aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatttc
     5941 catcccactg aaaccaaaag cactatcacc ttcaatggcg acaaccggag aaccagaagt
     6001 cacgctagca ccgatggcat agcccatacc gatgcccatg acaccccagg taccacaatc
     6061 cagacgacga cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc
     6121 accttcatta actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa
     6181 tgcattaaag taatttaatg gttgagtatc tgtacttaat ttttcatgca ttttttgtgc
     6241 attttgctgc ttgtggatat ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt
     6301 ttgtttcagt tctgccagca taccttgcat actggatgca atatcaccaa cgactggcac
     6361 agcaatgggg cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt
     6421 atctgccgcc catccttttt taccgtgtgc caataaccaa ttcagtcgtg caccaacaag
     6481 catgacaacg tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg
     6541 cgtatcttca aggatccctt tcgccataga cattggcagg aatggaatct gagcactttc
     6601 aataaattca cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc caaggataat
     6661 taatggccgt tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg
     6721 caataatgct ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc
     6781 catcgtcgcg gccaggacat ttgctggcaa atcaagataa actccgccag ggcgacccga
     6841 tacagagacc cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg
     6901 aaatgctgct ttggcatacg gttttgccgc attcatttgg tccagctctt cataatcacc
     6961 ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta atcatgatca tcggaaaacc
     7021 atttaccgtt gcgttggcca atgcggtcaa accattgagg aatcctggcg cagaaactgt
     7081 caggcagatc cccggttttt gggtaagaaa accgcttgcc gcagcggcat agcctgccga
     7141 ctgctcatga cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gtgccatatc
     7201 cgtcacagga atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc
     7261 aacgatgata tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga
     7321 aatttttata aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc
     7381 attgcagcaa tttcatcgtc gctataaccc agctcctgca atacagcagc ggtatgttca
     7441 cctaatagcg gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca
     7501 acggtcagat attttccacg caacggttgt tccacttcaa caacactgcc actttggcgc
     7561 aaagagggat caagtgaaat ttctttcata cttaaaaccg gtgcacaagg aatatcaaac
     7621 tgagtcaaat aggccaccgc ttcatgttta tcaatagtga cagtgtattt ttcgatttca
     7681 gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg tactgtatgc cgaatcggta
     7741 atccattctg gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg
     7801 aaataaatat aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc
     7861 tgaccgccac cacctgcatt accaccgcgg ggaactgcat caccaaatgt accattcgga
     7921 tactgcgggt attcttccag ataacccaat ttatcgagac gctgctggtc acgtaatttc
     7981 acgcggcaaa ggttcaatac ggcatcctgc attgacatgg tgactcgttg cccacgcccc
     8041 gttttttcgc gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg
     8101 ctgtcaccca acgctgcagc gcttaccagc ggcggaccat cccaaaaacc cgtagtggat
     8161 gccgcgccac ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa
     8221 cactcatcaa accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc
     8281 tcccaggtga agcccatgtg atcaatggcc cctggatgaa agttctcgac taagatatca
     8341 gcttcgcgga tcagcttttc cattacctct ttgccttccg ctgttttggt atttaactca
     8401 atagaacgtt tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct
     8461 cgcagttggt gacgcgttac gtcaccaacg ccgggacgtt caattttaat aacgtcagcg
     8521 ccaaaccagg ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga
     8581 actttaattc cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt
     8641 taatttagcg t
//
LOCUS       DAZPJP010000165         8632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPJP010000165 DAZPJP010000000
VERSION     DAZPJP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.166"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHU9J_003375"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHU9J_003375"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1906839.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHU9J_003376"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHU9J_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1906840.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHU9J_003377"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHU9J_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1906841.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHU9J_003378"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHU9J_003378"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1906842.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHU9J_003379"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHU9J_003379"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1906843.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHU9J_003380"
     CDS             complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHU9J_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1906844.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHU9J_003381"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHU9J_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1906845.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaat agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAZPJP010000166         8608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPJP010000166 DAZPJP010000000
VERSION     DAZPJP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.167"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(241..447)
                     /locus_tag="ACHU9J_003382"
     CDS             complement(241..447)
                     /locus_tag="ACHU9J_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1906846.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            760..849
                     /gene="kdpF"
                     /locus_tag="ACHU9J_003383"
     CDS             760..849
                     /gene="kdpF"
                     /locus_tag="ACHU9J_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1906847.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            849..2522
                     /gene="kdpA"
                     /locus_tag="ACHU9J_003384"
     CDS             849..2522
                     /gene="kdpA"
                     /locus_tag="ACHU9J_003384"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1906848.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHU9J_003385"
     CDS             2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHU9J_003385"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1906849.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHU9J_003386"
     CDS             4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHU9J_003386"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1906850.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHU9J_003387"
     CDS             5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHU9J_003387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1906851.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHU9J_003388"
     CDS             7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHU9J_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1906852.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1811 a         2225 c         2478 g         2094 t
ORIGIN      
        1 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
       61 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      121 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      181 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      241 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      301 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      361 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      421 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      481 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      541 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      601 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      661 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      721 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      781 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      841 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      901 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
      961 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1021 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1081 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1141 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1201 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1261 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1321 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1381 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1441 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1501 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1561 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1621 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1681 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1741 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1801 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1861 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1921 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     1981 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2041 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgattg
     2101 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2161 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2221 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2281 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2341 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2401 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2461 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2521 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2581 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2641 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2701 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2761 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2821 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2881 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     2941 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3001 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3061 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3121 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3181 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3241 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3301 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3361 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3421 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3481 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3541 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3601 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3661 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3721 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3781 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3841 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3901 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     3961 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4021 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4081 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4141 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4201 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4261 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4321 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4381 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4441 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4501 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4561 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4621 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4681 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4741 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4801 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4861 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4921 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     4981 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5041 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5101 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5161 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5221 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5281 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5341 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5401 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5461 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5521 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5581 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5641 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5701 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5761 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5821 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5881 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     5941 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6001 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6061 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6121 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6181 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6241 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6301 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6361 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6421 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6481 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6541 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6601 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6661 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6721 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6781 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6841 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6901 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     6961 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7021 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7081 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7141 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7201 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7261 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7321 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7381 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7441 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7501 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7561 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7621 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7681 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7741 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7801 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7861 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7921 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     7981 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8041 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8101 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8161 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8221 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8281 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8341 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8401 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8461 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8521 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8581 cgaatattat tatttggcag gtttaatt
//
LOCUS       DAZPJP010000167         8591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPJP010000167 DAZPJP010000000
VERSION     DAZPJP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.168"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHU9J_003389"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHU9J_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1906853.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHU9J_003390"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHU9J_003390"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1906854.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHU9J_003391"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHU9J_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHU9J_003392"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHU9J_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1906855.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHU9J_003393"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHU9J_003393"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1906856.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHU9J_003394"
     CDS             complement(6490..6846)
                     /locus_tag="ACHU9J_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1906857.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHU9J_003395"
     CDS             complement(6850..7266)
                     /locus_tag="ACHU9J_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1906858.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHU9J_003396"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHU9J_003396"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1906859.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2174 a         2145 c         2103 g         2169 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct t
//
LOCUS       DAZPJP010000168         8575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPJP010000168 DAZPJP010000000
VERSION     DAZPJP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.169"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(154..1818)
                     /gene="glnS"
                     /locus_tag="ACHU9J_003397"
     CDS             complement(154..1818)
                     /gene="glnS"
                     /locus_tag="ACHU9J_003397"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1906860.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2077..4023)
                     /gene="nagE"
                     /locus_tag="ACHU9J_003398"
     CDS             complement(2077..4023)
                     /gene="nagE"
                     /locus_tag="ACHU9J_003398"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1906861.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4356..5156
                     /gene="nagB"
                     /locus_tag="ACHU9J_003399"
     CDS             4356..5156
                     /gene="nagB"
                     /locus_tag="ACHU9J_003399"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1906862.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5216..6364
                     /gene="nagA"
                     /locus_tag="ACHU9J_003400"
     CDS             5216..6364
                     /gene="nagA"
                     /locus_tag="ACHU9J_003400"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1906863.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6373..7593
                     /gene="nagC"
                     /locus_tag="ACHU9J_003401"
                     /gene_synonym="nagR"
     CDS             6373..7593
                     /gene="nagC"
                     /locus_tag="ACHU9J_003401"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1906864.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7641..8393
                     /gene="nagD"
                     /locus_tag="ACHU9J_003402"
     CDS             7641..8393
                     /gene="nagD"
                     /locus_tag="ACHU9J_003402"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1906865.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8444..8575)
                     /locus_tag="ACHU9J_003403"
                     /pseudo
     CDS             complement(8444..8575)
                     /locus_tag="ACHU9J_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2097 a         2263 c         2184 g         2031 t
ORIGIN      
        1 accggcctac aaaagcacgc aaactcaata tattgcagag atcatgtagg cctgataagc
       61 gtagtgcatc aggcaattta gcgtttgaaa ctgtttgtct gttccttgcc ggatgcgacg
      121 taaacgcccc atccggcata gcgaaactta aaattactcg cctactttcg cccaggtatc
      181 acgcagccca acggtgcggt taaataccgg tttttccgcc gtagaatggc ggctatcgag
      241 gcagaagtaa ccttcacgct caaactggaa tgctttaccc gcaaccgcat ctttcagcga
      301 cggttcagca aagccctgtt tgatcaccag cgattccggg ttaatcaccg acaggaaatc
      361 atccgcagca ccagggttcg gcacgctgaa cagacggtcg tacagacgga tttcaaccgg
      421 cagcgcatgt gccgcgctca cccagtgaat aacacctttg actttacgac catctgccgg
      481 atctttgctt aaggtgtcgg cgtcataagt acagaagatg gtggtgatat tgccttcggc
      541 atctttctcc acgcgttctg ccttaatgac gtaagcatta cgcagacgca cttctttacc
      601 cagcaccaga cgtttgtact gcttgttagc ttcttcgcgg aaatcggcgc gatcgatcca
      661 aatctcaccg ctaaacggca cctggcggct acccatttcc ggtttgttcg gatggttcgg
      721 catggtaacc atttcgcctt cgccctgata gttttcgata accagtttca ccggatcgat
      781 aaccgccatt gcgcgcgggg cgttttcgtt gagatcttca cggatgcagg attccagcga
      841 cgccatctca atggtgttgt cctgcttggt cacgccgatg cgtttgcaga actcacgaat
      901 agaagcagca gtgtaaccac gacgacgcag accggaaatg gtcggcatac gcgggtcatc
      961 ccagccttca acgtgcttgt cggtcaccag caggttcagc ttacgcttgg acatcacagt
     1021 gtattccaga ttcaggcgcg agaattcgta ctggcgcggg tgaacaggaa tggtgatgtt
     1081 gtccagcacc cagtcgtaca ggcgacggtt gtcctggaac tcaagcgtac acagagagtg
     1141 cgtaatacct tccagggcat cgctgatgca gtgggtgaag tcgtacatcg ggtagatgca
     1201 ccacttgttg ccagtctggt ggtgttcagc aaatttaatg cggtacagca ccggatcgcg
     1261 catcacgata aacggtgaag ccatgtcgat tttcgcacgc aggcaggctt taccttcttc
     1321 aaaaccaccg gcacgcattt tttcgaacag cgccaggttc tcttcaacgc tgcggtcgcg
     1381 gtacgggctg tttttacccg gttgcgtcag ggtgccgcgg tattcgcgga tctgttccgg
     1441 cgtcagttca tcaacgtacg ccaggccttt attgatcagt tcgatcgcat aggcgtggag
     1501 ctgatcaaaa taatcggagg agtaacggac gttaccagac cagtgaaaac ctaaccactc
     1561 tacgtcgttt ttgatcgact caacatactc gatatcttct tttaccgggt tagtgtcgtc
     1621 gaaacgcagg ttgcactggc ctttatagtc ctgggcgatc ccgaagttca ggcagataga
     1681 tttcgcatgg ccaatatgca gatagccatt cggctccggc gggaaacggg tgtgtactgt
     1741 ggtgtgctta ccactggcca gatcttcatc gatgatctga cggataaagt tagtcgggcg
     1801 ggcttctgcc tcactcatcg tggattcctc aaagcgtaaa caacgtataa cggcgtatga
     1861 tcttataagc gggacaggct gacaactgtt agttagcgaa aatcgaaaat ctcactgaga
     1921 ttatggggat gattgatgaa aaaaaaagcg gcagagaaga tctccaccgc ttttgccagt
     1981 cccgcgtcat tgttggatgc gcgtcttatc caaccaggac tgagcttttg taggcctgat
     2041 aagacgcgtc aagcgtcgca tcaggcataa agcagattac tttttgattt catacagcgg
     2101 tgtttgaccc gccacaacat tgccctgagc tttaatgatc aagccactga aatcgtcgat
     2161 attgctgcaa accaccgggc taatcatcga acgggcgtta gcgttcaggt aatccagatc
     2221 catttccaga atcggttgcc ctgcgctcac ctgcgcgccc tcttccacca gacgtttaaa
     2281 gcctttacct tccagcgcta cggtgtcgat acccatgtgg acgacaatct ccgcgccttt
     2341 ttcggtttct aggcagaacg cgtggttggt gttgaagatt ttcacgattg tccctgcggc
     2401 tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca ccgctttgct
     2461 ggcgaatgct tcgtcagaaa cctgatccag tgccacgaca tcaccggtaa tcggcgatac
     2521 cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag gagcggcagt
     2581 tgccggagtt gcttcagctg acgcagctgc taccgggcca cgggcaacga ctttcttcat
     2641 cgcatcgcca atggattctg ctttcgcgcc aacaatcacc tgaatggtct gtttgttcag
     2701 ttttactaca ccagaagcac ccagacgttt acacatcgca tcgttgacgc gagctgagtc
     2761 agccacggta aggcgcagac gggtaataca ggcgtcaatc gctttcaggt tgtcagtacc
     2821 gccaaccgca gcaatatagt tggttgccag ttgagtcaga ccttcttcag tattgctgtt
     2881 agcctcttca gttacgatct cgtcttcttt atcttcacga cccggcgttt tcaggttgaa
     2941 catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa cacccatcac
     3001 cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg cgtagtcgat
     3061 agcccccgca gagaaagaga agccagcatg gatacccagc agcgttgcca caaacaggct
     3121 gataccggtc agcagtgcgt gcaggaggta cagcagcgga gcaaggaaca tgaacaggaa
     3181 ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc
     3241 gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg
     3301 cagaccgaac atcatgatcg ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc
     3361 agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc gccgcgttgg tgaattcacc
     3421 aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga tcagcagacg
     3481 gttgatgaaa ccaaagatac cggaacccag cgcgcctgca gaaacgatcc actcgccgcc
     3541 tgcatggata gcgtgctgta ctggcggcca gacgtaacca aaaatggccg ccagcaccag
     3601 gcagaagaag ccggtggcaa tcggcacaaa gcgtttgccg ccgaagaagc tcaggaagtc
     3661 cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac cggtaatgat
     3721 acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt tggttaacac
     3781 aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg accagctgga
     3841 tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc
     3901 aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg ccgccaccgg
     3961 cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt
     4021 catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt tttattttgc
     4081 tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgct acagagtgtg
     4141 tgaaaattta attcgtatcg caaattaaac gcgtgccttt tgtgagtttt gtcaccaaat
     4201 atcgttatca tcactccctt ttactggcta aagcagaaaa cttattttat cattcaaaaa
     4261 atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc
     4321 gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc ctaactaccg
     4381 ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac
     4441 cgactgccga tcgtccgttt gttctgggcc tgccgactgg cggcacgccg atgaccacct
     4501 ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac gttgtcacct
     4561 tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac tacagcttta
     4621 tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac cttctcaacg
     4681 gcaacgcccc ggatatcgac gccgaatgcc gccagtatga agaaaaaatc cgttcttacg
     4741 gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca tttaacgaac
     4801 cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac actcgcgtcg
     4861 cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg
     4921 gtgttggtac attgctggat gccgaagaag tgatgattct ggtgctgggt agccagaaag
     4981 cactggcact gcaggctgca gttgaaggtt gcgtgaacca catgtggacc atcagctgtc
     5041 tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc ttccaccatg gagctgaaag
     5101 ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt
     5161 tatccctgcc ctggctcctt gctcagggca atatttttta aaatcggggg gcagaatgta
     5221 tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc acgcggttgt
     5281 tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc cagagatcga
     5341 acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc agttaaacgg
     5401 ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat
     5461 catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc ttatcaccac
     5521 cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg caagacatcc
     5581 gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa aaaaaggcac
     5641 ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa
     5701 cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg aagtcatcag
     5761 caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga
     5821 agcgaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca acgcgatgcc
     5881 gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag ctgacattta
     5941 ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta acgctaaacg
     6001 ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag gtgccaacat
     6061 tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt gtgtggatga
     6121 gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta atctggtcga
     6181 acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc
     6241 gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca acctgacctc
     6301 attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg tcgtaactca
     6361 ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga tctcgtaaaa
     6421 cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat ctcgcggatt
     6481 cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt
     6541 atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg ccgccgcgct
     6601 atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac
     6661 gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga acattacccg
     6721 ctgccggaac gtacccagca aacgctggaa catgccctgt tgaatgccat tgctcagttt
     6781 attgatagct accagcgcaa actgcgcgag ctgatcgcga tttcggtgat cctgccaggg
     6841 cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt agaaaactgg
     6901 gggctggtag aagctctgga agaacgtttt aaagtgacct gtttcgttgg tcacgatatc
     6961 cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga agactccatt
     7021 ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg catttttatt
     7081 ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct gggtgaacgc
     7141 tgccactgcg gcaactttgg ctgcctggaa actatcgccg ccaacgctgc cattgaacaa
     7201 cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga cgactgcacc
     7261 atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc tggcgtcgga agtaattgag
     7321 tatgtcggtc gtcatctggg taaaaccatc gccattgcta tcaacttatt taatccgcaa
     7381 aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc tgctattgaa
     7441 agctgcatta atacccaggc gctgaaggcg tttcgtacta atctgccggt ggtacgttct
     7501 gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc catgctcaac
     7561 ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc gcttattgtt
     7621 gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc
     7681 tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg attatggata
     7741 aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa gatctggcga
     7801 accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc tctgcgatgg
     7861 cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc atatgtggtg ggcgaaggcg
     7921 cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac cctgatttcg
     7981 tgattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg gcctatttcg
     8041 tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc ggtttttatc
     8101 ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa ccgttctatg
     8161 ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa caaaatgcag gcgcattcgg
     8221 aagaaacggt gattgtcggc gataacttgc gcaccgacat tctggcgggc ttccaggcag
     8281 gtctggaaac gattctggtg ctttctggtg tttcatcgct cgacgatatc gacagtatgc
     8341 cgttccgccc aagctggatt tacccgtcag tcgctgaaat cgacgttatc tgaataccac
     8401 ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca aactgcctga tgcgctacgc
     8461 ttatcaggcc tacgcagctc ctgcaatata ttgaattttc atgcttttgt aggccggata
     8521 aggcgttcac gccgcatccg gcatgaacaa atcgcacttt atcagcaatt tggcc
//
LOCUS       DAZPJP010000169         8526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPJP010000169 DAZPJP010000000
VERSION     DAZPJP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.170"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(145..2136)
                     /gene="tkt"
                     /locus_tag="ACHU9J_003404"
     CDS             complement(145..2136)
                     /gene="tkt"
                     /locus_tag="ACHU9J_003404"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1906866.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2414..3172
                     /gene="loiP"
                     /locus_tag="ACHU9J_003405"
                     /gene_synonym="yggG"
     CDS             2414..3172
                     /gene="loiP"
                     /locus_tag="ACHU9J_003405"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1906867.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3315..4235)
                     /gene="speB"
                     /locus_tag="ACHU9J_003406"
     CDS             complement(3315..4235)
                     /gene="speB"
                     /locus_tag="ACHU9J_003406"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1906868.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4373..6349)
                     /gene="speA"
                     /locus_tag="ACHU9J_003407"
     CDS             complement(4373..6349)
                     /gene="speA"
                     /locus_tag="ACHU9J_003407"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1906869.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6358..6489)
                     /gene="yqgB"
                     /locus_tag="ACHU9J_003408"
     CDS             complement(6358..6489)
                     /gene="yqgB"
                     /locus_tag="ACHU9J_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1906870.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6583..6726)
                     /gene="yqgG"
                     /locus_tag="ACHU9J_003409"
     CDS             complement(6583..6726)
                     /gene="yqgG"
                     /locus_tag="ACHU9J_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1906871.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6837..7088)
                     /gene="yqgD"
                     /locus_tag="ACHU9J_003410"
     CDS             complement(6837..7088)
                     /gene="yqgD"
                     /locus_tag="ACHU9J_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1906872.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7144..8298
                     /gene="metK"
                     /locus_tag="ACHU9J_003411"
     CDS             7144..8298
                     /gene="metK"
                     /locus_tag="ACHU9J_003411"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1906873.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2073 a         2300 c         2187 g         1966 t
ORIGIN      
        1 tcgtgatgca tattacataa ttgatattta ttgcatataa atatttgtga tctacaacac
       61 gctttatcta ttgcttgtcc gcaaacggac atatcaaggt aataaaaaag gtcgccgaag
      121 cgaccttttt acccgaaatg ctaattacag cagttctttt gctttcgcaa cgacgttgtc
      181 gacggtgaag ccgaactctt cgaacagcaa ctctgccgga gctgactcac cgaaggtggt
      241 cataccgatg atagcgccat tcaggccaac atacttgtac cagtagtcag caatacccgc
      301 ttctaccgca acgcgtgcag taaccgcttt cggcagtacg gattcacggt aagccgcatc
      361 ctgcttgtcg aatgcatcgg tagacggcat ggaaaccacg cgcgccttca cgccttcggc
      421 agtcagtttt tcgtaggcgg caacagccag ttcaacttcc gaaccggtcg cgatgaagat
      481 cagttccggc tgaccagcac agtctttcag cacataacca ccgcgcgcga tgtttgccag
      541 ttgctcttca gtacgttcct gctgcgccag attctgacgg gagaggataa gcgcggtcgg
      601 accgtcctga cgctcaacac cgtatttcca cgcgaccgcg gattcaacct ggtcacacgg
      661 acgccatgta gacatgttcg gggtcacgcg cagagaagcg acctgctcaa ccggctggtg
      721 agtcgggccg tcttcgccca gaccgatgga gtcgtgggtg taaaccatca cctgacgctg
      781 tttcatcagc gcagccatac gtacagcgtt acgtgcgtat tcgacaaaca tcaggaaggt
      841 ggaggtgtac ggcaggaagc caccgtgcag ggagataccg ttagcaatcg cggtcatacc
      901 gaactcgcga acaccgtagt ggatgtagtt acccgcagca tcttcgttga ttgctttaga
      961 accagaccac agggtcaggt tagacggtgc caggtcagcg gagccgccga ggaattccgg
     1021 caacagcggg ccgaacgctt caatagcatt ctgagacgct ttacggctgg cgattttcgc
     1081 cggattagcc tgcagtttag cgataaactc tttcgctttg gcgtcgaagt cagacggcat
     1141 ttcgcctttc atacggcggg taaattcagc cgcttcctgc ggataagctt tcgcgtaagc
     1201 cgcaaacttc tcattccatg cagattcttt cgcctggcct gcttctttcg catcccactg
     1261 cgcatagatt tcagacggga tttcgaacgg tgcgtatttc cagcccagct gttcgcgggt
     1321 cagagcgatt tctgcatcac ccagcggcgc accgtgggag tcgtgagtac cggctttgtt
     1381 cggggaaccg aaaccgatga tggttttgca catcagcagg gacggtttgt cagtcactgc
     1441 gcgcgcttct tctactgcgc gtttgatgga tgctgcgtca tgaccgtcga tgtcgcgaat
     1501 aacgtgccag ccgtaagctt cgaaacgcat tgcggtgtcg tcggtgaacc agccttcaac
     1561 gtgaccatca atagagatac cgttgtcatc gtagaacgca atcagtttac ccagcttcag
     1621 cgtacccgcc agagagcaaa cttcgtggga gatgccttcc atcatgcagc cgtcgcccat
     1681 gaaggcgtag gtgtagtggt cgacaatgtc gtggcccgga cggttaaact gcgccgccag
     1741 cgttttttct gcaatcgcca taccgactgc gttggcaata ccctgaccca gcggaccggt
     1801 ggtggtttcc acaccagcgg tataacctac ttccgggtgg cccggagttt tagagtgcag
     1861 ctgacggaag tttttcagtt cttccatcgg cagatcgtaa ccggtgaggt gcagcaggct
     1921 gtagatcagc atggagccgt ggccgttgga cagcacgaag cggtcacggt cagcccagga
     1981 cggattctgc gggttgtgtt tcaggaaatc acgccacagg acttcggcaa tgtcagccat
     2041 acccataggg gcacccgggt gaccggattt ggctttctgt actgcgtcca tgctcagcgc
     2101 acgaatagca ttggcaagct ctttacgtga ggacattttg actccagatc ggatgatgaa
     2161 gggcacgccc ttaacgactt gacgacagcg cgttttgggc tacgccggaa aatttgccaa
     2221 caatttaccg caagccgcgc gtcatgtaca tggaacatcc ttttgccgct tcagaaatct
     2281 ctggatcatg ctcgcatgtt gcgcaatcta ctcgcccgtc cgctgcgctt ttccttatac
     2341 tgagactgag cgtcgattca cctgcaaacg gcgcattttt agaataatcc tgaccttgtg
     2401 cggaagagaa aacatgaaaa ttcgcgcctt attggtagca atgagcgtgg caacggtact
     2461 gactggttgc cagaatatgg actccaacgg actgctctca tcaggagcgg aagcttttca
     2521 ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc gatcaggcat gtcaggagat
     2581 ggacagcaag gcgacgattg cgccagccaa tagcgaatac gctaaacgtc tgacaactat
     2641 tgccaatgcg ctaggcaaca atatcaacgg tcagccggtg aattacaaag tgtatatggc
     2701 gaaggatgtg aacgcctttg caatggctaa cggctgtatc cgcgtctata gcgggctgat
     2761 ggatatgatg acagataacg aagtcgaagc ggtgatcggt cacgagatgg ggcacgtggc
     2821 gttaggccat gtgaagaaag ggatgcaggt ggcgcttggt acaaatgccg tgcgagtagc
     2881 tgcggcctct gcgggcggga ttgtcggcag tttatctcaa tcacaacttg gtgatctggg
     2941 cgagaaatta gtcaattcgc aattctccca gcgccaggaa gcagaagccg atgattattc
     3001 ttacgatctt ctgcgccaac gcggcatcag cccggcaggt cttgccacca gctttgaaaa
     3061 actggcgaaa ctggaagaag gccgccaaag ctcaatgttt gacgaccatc cggcatccgc
     3121 cgaacgcgcc cagcatattc gcgatcgcat gagcgcggat gggattaagt aaagcctggt
     3181 ggtgtcgaac gtaggtcaga taaggcgttc acgccgcatc tgacatcaat ggcacatttg
     3241 ttaccctgtg cgcattgccg gatgcgatgc tgactgcatc ttatccggcc tacgggtggc
     3301 atcgcatctg gtgcttactc gccctttttc gccgcttgga tatacagcat ctccagcgcc
     3361 agcgtcgccg cagccagagc ggtgatttcc gactgatcgt atgccggagc cacttccact
     3421 acgtccatcc caacaatgtt gagatctttc aggccgcgta ccagtttaat agcgcgatcg
     3481 gaggtcaggc cgccaatcac tggcgtaccg gtgcctggtg caaaagcagg atccaggcag
     3541 tcgatatcaa aagtcaggta aaccggcata tcacccacaa tctgtttcac ttgggcgata
     3601 acgtcatcca cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa gccgttgtct
     3661 ttatcaaact cggtacgaat accaatctgc acggaatgat tcgggtcgat cagaccttct
     3721 ttcggcgcgg tatagaacat agtgccgtgg tcaaattcac aaccgttcgc ataggtatcg
     3781 gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc
     3841 agcggcagcg taacaaagtg gtcaccaccg aaggtgagca tacgcttacc ggcagccagc
     3901 agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc aaaggcatat
     3961 accagatcgc cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt ccacgggaag
     4021 cggttgtgtt cccaggccag attcgtcgaa acctgacgga tcgctgccgg accgtggcga
     4081 ccacccgcac gaccagaagt ggccatatcg aacggcacgc cagtaatcac ccagtctgca
     4141 tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc attggaaacc
     4201 agtgagttat cgtattgatg acctaaggtg ctcatggatg gactcctatt acaaagtcga
     4261 tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa gggttggatt tgtcacaata
     4321 aattgtggcg gattatcacc gctaaattaa gcggattcaa gtaacacagg acttactcat
     4381 cttcaagata agtataaccg tacaaacctg cctcgaactc ttcaaggaac tgttgttgca
     4441 gttcagcatc aagatcggtt ttcttcactt gatcgcggaa ctgggttaac agcgttttcg
     4501 gatcgagctg tacatattgc agcatgtccg ccacggtatc gccttcgtca gacagttcta
     4561 cttctacgct accgtcaggg aacacgaaca cgtcaaccgc ttcggtatca ccgaacaggt
     4621 tgtgcatatt gccaaggatc tcctgatatg cgccgaccat aaagaaaccg agcatcggcg
     4681 gattctctgg atcgtactcc ggcattggca ttgtcgtggc aataccatca ccatcaatat
     4741 agtggtcgat agcaccgtca gagtcacagg taatatccag cagcacagcg cgacgttccg
     4801 gcacttgatc cagcccttcc agcggcagaa ccgggaacaa ctggtcgatc ccccatgcgt
     4861 ccggcattga ctggaacagc gagaaattga cgtacatttt gtccgccata cgttcctgca
     4921 gttcgtcgat tatcggacga tgcgcacggt tttgcggatc cagttgcttc tgcacttcat
     4981 ggcacatgct cagatagagt tgttctgccc atgcacgttc ttgcaggcta aaggtgccgg
     5041 aagagtagcc gatatgaatg tcgtgcagat ccatctggct gtcgtgtaac cattcacgca
     5101 aagaacggcg agttcccggt tcgtgcattt cctgccaggt ttcccacatg ctttgcagcg
     5161 cgcgcggcgc atcttctgcg ggcgcggtcg gcaccgtata ttcgttacgt tccacgccga
     5221 tgatattcga caccagcacg gtatggtgcg cagtcaccgc acggcccgat tcggtgatta
     5281 ccgtcggatg cggcagacca ttttcttcac acgcatcgcc aatcgcccag atgatgttgt
     5341 tggcgtattc attgaggcca tagtttacgg agcagtcaga ctgcgaacgg gtgccttcat
     5401 agtccacgcc cagaccgccg ccgacgtcga agcactgaat attgacgccc agcttgtgca
     5461 gttccacata gaaacgcgcg gattcacgaa cgcccgtggc gatatcgcga atattcgcca
     5521 tctgcgaacc gaggtggaag tgcagtagtt gcaggctgtc gagacgcccg gcttcacgca
     5581 gggtttcaac cagttgcagt acctgagtcg cagccaggcc gaacttcgat ttttccccgc
     5641 cggaggactg ccatttaccc gagccctgcg aagccagacg tgcacgcacg cccagacgag
     5701 gaacgacatt cagacgttct gcttcatcca gcacaatggc gatttctgac atcttctcaa
     5761 tgaccagata gaccttgtgc cccatcttct cgccaattaa tgccaggcgg atatattcgc
     5821 ggtctttata accgttgcag acgatgacgc tacgggtcat gccagcatgt gccagtactg
     5881 ccatcaactc ggctttggaa ccggcttcca gacccagcgg ttcgcccgaa tgaatcaggg
     5941 actcaatcac gcggcggtgc tggttaactt tgatcggata aacaaggaag taatcgccgt
     6001 tatagccgta ggattccctc gcacgtttga acgcggcgtt aatggaacgc aaacggtgct
     6061 gcaggatctg tgggaaacag aacagtgcag gcagacgctg accctgtgct tcacgagttt
     6121 tcactaactg cgcgagatcg acgcgagctt ccgggacgtc cgggtccggg cacacgctga
     6181 tgtggcccag ctcgttaacg tcatagtagt tattgcccca ccaggcaata ttgtaagtac
     6241 gcagcatctt gctggcttcc tgggagctca ttgcaacctc ctgcatggag cgtagtacac
     6301 cgtgttcgcc cgctgacgaa ggcaaaccca tagacatgtc gtcagacata gcgaacctca
     6361 aattatttta ttaagtgtaa aacagttaac gactatcgca gcctggaact gcgataacaa
     6421 cccataagca catggatttt ccagcagtga atgctgacgc tccaactgcg caaccggttt
     6481 ctttttcata acattattaa gcacataacc gaacgtaagt gtgacagttc ggcgaaacca
     6541 cgagaaaact cttgttttta caagagcgcc cttgttcagt cctcagtaac tgtaaccagc
     6601 tcttgaatcc tgagaagcgc cgagatgggt ataacatcgg caggtatgca aagcagagat
     6661 gcagagtgcg gggaacgaat cttcaccaga acggtgagac aggttaagca gcagacaacg
     6721 gttcattatt tcgtatcacc tccacggccg cctgttaaga cgaacccaca agccaaaact
     6781 ctgatttcaa cccggctgga agtggcaaca cgaaagaaac gtcgtgtgct ttttatttaa
     6841 gccgcgcgcc gcgttttata ccccacaatg gcagaaaatt gcaaaagata aatacgcaga
     6901 atgccggcat tgtcaggaaa aatttccagc cacgttttta acagaatgag acacgattca
     6961 aaaaaaagtg gaaatcgggt gaagaattga cctaaaatag ccatccagat gttaatccat
     7021 ccataccgat taacactcag actgccagtg tttttaacct gcagagtcgt ggtaggatcc
     7081 gctaccacag aaaatccaca caacagtttg agctaaccaa attctcttta ggtgatatta
     7141 aatatggcaa aacacctttt tacgtccgag tccgtctctg aagggcatcc tgacaaaatt
     7201 gctgaccaaa tctctgatgc cgttttagac gcgatcctcg aacaggatcc gaaagcacgc
     7261 gttgcttgcg aaacctacgt aaaaaccggc atggttttag ttggcggcga aatcaccacc
     7321 agcgcctggg tagacatcga agagatcacc cgtaacaccg ttcgcgaaat tggctatgtg
     7381 cattccgaca tgggctttga cgctaactcc tgtgcggttc tgagcgctat cggcaaacag
     7441 tctcctgaca tcaaccaggg cgttgaccgt gccgatccgc tggaacaggg cgcgggtgac
     7501 cagggtctga tgtttggcta cgcaactaat gaaaccgacg tgctgatgcc agcacctatc
     7561 acctatgcac accgtctggt acagcgtcag gctgaagtgc gtaaaaacgg cactctgccg
     7621 tggctgcgcc cggacgcgaa aagccaggtg actttccagt atgacgacgg caaaatcgtt
     7681 ggtatcgatg ctgtcgtgct ttccactcag cactctgaag agatcgacca gaaatcgctg
     7741 caagaagcgg taatggaaga gatcatcaag ccgattctgc ccgctgaatg gctgacttct
     7801 gccaccaaat tcttcatcaa cccgaccggt cgttttgtta tcggtggccc gatgggtgac
     7861 tgcggtctga ctggtcgtaa aattatcgtt gatacctacg gcggcatggc acgtcacggt
     7921 ggcggagcat tctctggtaa agatccatca aaagtggacc gttccgcagc ctacgcagca
     7981 cgttatgtcg cgaaaaacat cgttgctgct ggcctggccg atcgttgtga aattcaggtt
     8041 tcctacgcaa tcggcgtggc tgaaccgacc tccattatgg tagaaacttt cggtactgag
     8101 aaagtgcctt ctgaacaact gactctgctg gtacgtgagt tcttcgacct gcgcccatac
     8161 ggtctgattc agatgctgga tctgctgcac ccgatctaca aagaaaccgc agcatacggt
     8221 cactttggtc gtgaacattt cccgtgggaa aaaaccgaca aagcgcagct gctgcgcgat
     8281 gctgccggtc tgaagtaatc tttcttcacc tgctttcaaa ggccagcctc gcgctggcct
     8341 ttttctttta cctcaccgcg gtggataggc attcacgccg catccggcaa aaagctgccc
     8401 gcacaataac atcattcttc ctcaacacgt tttatcgcaa attatcatca ctactgaaac
     8461 cgattacacc aataacacca gccaaatatt tgtaatattt tcatattatt attcagtttt
     8521 cacagt
//
LOCUS       DAZPJP010000170         8507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPJP010000170 DAZPJP010000000
VERSION     DAZPJP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.171"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHU9J_003412"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHU9J_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1906874.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHU9J_003413"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHU9J_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1906875.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHU9J_003414"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHU9J_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1906876.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHU9J_003415"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHU9J_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1906877.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHU9J_003416"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHU9J_003416"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1906878.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHU9J_003417"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHU9J_003417"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1906879.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHU9J_003418"
     CDS             complement(6574..7338)
                     /locus_tag="ACHU9J_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1906880.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHU9J_003419"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHU9J_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1906881.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
BASE COUNT         2047 a         2387 c         2198 g         1875 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacatt
//
LOCUS       DAZPJP010000171         8478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPJP010000171 DAZPJP010000000
VERSION     DAZPJP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.172"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            32..1012
                     /locus_tag="ACHU9J_003420"
     CDS             32..1012
                     /locus_tag="ACHU9J_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1906882.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1005..1421
                     /locus_tag="ACHU9J_003421"
     CDS             1005..1421
                     /locus_tag="ACHU9J_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1906883.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(1423..2697)
                     /locus_tag="ACHU9J_003422"
     CDS             complement(1423..2697)
                     /locus_tag="ACHU9J_003422"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR1906884.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(2697..3134)
                     /gene="umuD"
                     /locus_tag="ACHU9J_003423"
     CDS             complement(2697..3134)
                     /gene="umuD"
                     /locus_tag="ACHU9J_003423"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1906885.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(3131..3379)
                     /locus_tag="ACHU9J_003424"
     CDS             complement(3131..3379)
                     /locus_tag="ACHU9J_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1906886.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            3797..4699
                     /locus_tag="ACHU9J_003425"
     CDS             3797..4699
                     /locus_tag="ACHU9J_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR1906887.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TEQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            complement(4650..4871)
                     /locus_tag="ACHU9J_003426"
     CDS             complement(4650..4871)
                     /locus_tag="ACHU9J_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFR1906888.1"
                     /translation="MPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRHPLS
                     EKKRAVSRPSPSYFIRHNAPHCRLSTQVR"
     gene            4796..5008
                     /locus_tag="ACHU9J_003427"
     CDS             4796..5008
                     /locus_tag="ACHU9J_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906889.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            5084..5767
                     /locus_tag="ACHU9J_003428"
     CDS             5084..5767
                     /locus_tag="ACHU9J_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1906890.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNHHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            5768..5989
                     /locus_tag="ACHU9J_003429"
     CDS             5768..5989
                     /locus_tag="ACHU9J_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906891.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            5970..6436
                     /locus_tag="ACHU9J_003430"
                     /pseudo
     CDS             5970..6436
                     /locus_tag="ACHU9J_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            6487..7257
                     /locus_tag="ACHU9J_003431"
     CDS             6487..7257
                     /locus_tag="ACHU9J_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906892.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSECIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            7674..8099
                     /locus_tag="ACHU9J_003432"
     CDS             7674..8099
                     /locus_tag="ACHU9J_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1906893.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGRDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            8146..>8478
                     /locus_tag="ACHU9J_003433"
     CDS             8146..>8478
                     /locus_tag="ACHU9J_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR1906894.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLAD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSE"
BASE COUNT         1879 a         2201 c         2370 g         2028 t
ORIGIN      
        1 tctatttgtt tatgagcaac ggagactggt tatgaaaatc tttattgatg atggttctac
       61 caacattaaa cttgcctggt tggaggatgg tgacgtgaaa acgttaatca gccctaacag
      121 ctttaaaccg gaatggtctt ttagtttact ggatgatgct gccccggcta attacgagat
      181 cgacggcgag aagttttcat ttgatccatt aagtgctgat gctgtcgtaa cgactgaaac
      241 acgctatcag tacagtgatg tgaatgttgt ggccattcag catgcgctac agcagaccgg
      301 cctgaaagcg cagcctgttg atgtcatcgt cacattgccg atctctgaat accttgacgc
      361 caacaatcag aaaaataagc aaaacattga aagaaagaaa aaaaatgtga tgcgtgaagt
      421 acgggtgcag gggagtgatg catttgttat tcgcagtgtt tccgttcttc ctgaaagtat
      481 tcccgctggt ttcagtgttc ttgcggggct tgaggatgat gaatcccttt tgatcgtaga
      541 tctcggtggt acaactcttg atgtgtcgca tgttcgtagc aaaatgacag ggattacaaa
      601 aacctggtgt gatccaaata tcggcgtgtc cctgattaca tccggggtta aggaacagat
      661 ggctgtacat gctaatacaa gagtgagctc attccaggct gacaatatca ttgtgcaccg
      721 taatgaacct gactaccttt cccgtcgcat ttataatgct gaacagcgtg agtctatcat
      781 taatgttatc aatgaacggc agaagttgct tattaaacga gttaatgatg tcatcagccg
      841 tttcaccgac tatacacatg taatgtgtgt tggtggtggg gctgaaattg tggccgaggc
      901 tgtgaagaac ctgactaagg ttcctgatga gcgcttttat ctgagctcat ccccgcagtt
      961 tgatttagtc atgggaatga taaaaatgaa aggtggtgtg actaatgagt gatgaaaata
     1021 aatccagacg ctgctcattt gaacttttcc cggatgaacg gacaggagat aagatagcag
     1081 atgagctcat tgcaaatgaa aaactgaagg agcggggacg tttcatgcgc gcgatgttag
     1141 ttactggtgc ggcgtttgca gctatagata aacgtcttcc tttgctgata tcggagttac
     1201 tgacagaaaa tacgactctg gatgatatta ataaggtgat ttccagcgtg ataccaggtg
     1261 ctttttcggt tgaaaagaaa ttgcttgaat tactggaaaa acaatctggt ctccatacgt
     1321 ctgtggattg cagtactcct cttacagagc agagtctgtc gagaaatgac ggtgaagacc
     1381 agaccagaag aaacgctgaa aatatgttcg gagatgactg aactaaccca gcctggccag
     1441 gggaatatct cgccatttag ttgtataaca ctgactcaac atttcccgtt tcatttgcca
     1501 ttcaggggcg gttcctcgcc ccgcaaacca cactttccct tttcccgact ggttcagttc
     1561 atcgagtgtt ttcattaact tttcactgtt tttacggggc tggatttcat caaataatcc
     1621 cggctgcgct atacccgatg gtgtgaaatc agccagcatg actcctgcct tcatatagcg
     1681 gtacccttca cgccagacat ggtttaaggc tctgcatgcg gcggcaataa tgtcccggct
     1741 gtcctgtgtg ggcaatggaa gcttttccac agcggcattg ctgtaacagg gttcttttac
     1801 tgcaaagggg gatgtccgta caaatgtcgt cacctgccgg caatactgac gctccccacg
     1861 tagtttctct gcggcccgct ctgcatactg aacaacagcc tggtgcatgg catctttgtc
     1921 tgtgattcgt tcaccaaaac tgcgactaca gacaatctgc tgttttgccg gtggtgcttc
     1981 ttccagggat atgcaggact cgccgttgag ttcgcgtacc gtacgctcaa gaatgacgct
     2041 gaagtttttc cggatgaatg ccgtgttagc ctgcgccagc tgcagtgctg tgttaatacc
     2101 cagcgcattc agcttttccg tcagtctgcg tcctactccc cagacctcac caactggctg
     2161 cagtcccagt agcttcaaga tccgattacg gttttctgcc gtcagcgcga ccacaccgga
     2221 aaactgtggc cattgctttg ttgcccactg tgcactttta gccagcgttt ttgtaggcgc
     2281 aatgcccacc cccatggtga gtcctgtcca gctctttacc tgttccctga gctgatgacc
     2341 aaaaaactcc ggagagatgc aatgatttat cccccgcaaa tcaataaaca tttcatcaat
     2401 tgagtagggc tcaactgcgg gagaaagcga ctccagaaca gccatgaccc gttggctcat
     2461 gctgtggtac agcgcataat tgctggaaaa tacatgtatt ttcttctcca ggcgcatctg
     2521 tctcacctga aaccagggct gccccattct gatgccaagg gcttttgcct ccgggctgcg
     2581 cgcgatcaca cagccatcgt tattgctgag tacgatgacc ggttcgttgc gaaggtccgg
     2641 gcggaaaact ttttcacatg aggcgtagaa actgttgata tcagccagtg caaacatcag
     2701 cgtaactccc gcgtcctgtg tatcacgtga gtgacaacac caaaaataca gatgttttcc
     2761 ggatacagtg tgcggaactc cgggctgtct gaaaccggct ccagtgccgg gcgtgggcgc
     2821 aacagcagtc gtttgacggt gaactcaccg tcgatctcag cgataacgat gtccccgtgt
     2881 tgtggttttt cggccctgtc cactaccagc agatcaccat tctgcacgcc agcctggttc
     2941 atcgattcac cgctggcgcg cagaaagaag gtggctgcag gtctgctgat gcaatagctg
     3001 ttcagatcca gttcctgctc agcataatca gtggcaggcg aaggaaaacc ggcctggcaa
     3061 cgatcggcaa acaacggtcg cacgtaactg tcatcgcctg aagggtcagc cggacggtga
     3121 taaacggtac tcatgctgca ggcagccagc tgtcgtcctg ccagatttct tccagcagag
     3181 acaggatacg ttctttatcc ttgtcgttgc gggctcctgt gacagataca gacggggcgc
     3241 tgccctggtc tatacgcagc cagatttccg ggtagtgtgg tttcagacgt tgttcgattt
     3301 cattctgtag cgcctgaagt gtgcctgatt tgaggttttt tgtgctctgg cggtcgaaaa
     3361 gaatttcaat gcggatcatg ttctcatctc ccttttctgt tgctgtatgc atatacagta
     3421 ttctaataac tgaatccaca cacagtcaaa tgtcattatt gtgactaaaa aacgctcaat
     3481 tctctgagag tgtggctgta tgcctgctgt ggactgctgt gtccagctta tccacagcat
     3541 tttgcgcacg gttctgtgaa taaaatacct ggttacccag gccgtgccgg cacgttaacc
     3601 gggctgcatc cgatgcaagt gtgtcgctgt cgccggcctc ctcacccggt cacgtttcgt
     3661 cgttcctcct ccacgcgctg cggcttcggg gccgcacctg cattcgtatg cggtcgcccg
     3721 gttacaggtg cggcacggcc tgatggaggc cgcatgtgag aggagaattc ccatgccaaa
     3781 ctggtgctcg aatcgtatgt atttttctgg tgaaccggca cagatcgctg agattaaacg
     3841 actggccagc ggcgcagtca caccgtttta tcgccgcgcc acaaatgaag gtattcagct
     3901 gtttctggcc ggaagtgccg gacttctgca gaccactgaa gatgtgcagt ttgaaccgtg
     3961 ccccggactg acggctgccg gacgtggtgt tgtatcgccg gagaatatcg cgttcacacg
     4021 ctggctgaca cacctgcaga acggtgtgct actggatgaa caaaactgcc tgatgctgca
     4081 tgaactctgg ctgcagagcg gtactgaaca gcgtcgctgg gaaggattac cggacgatgt
     4141 cagggatacc atcaccgcac ttttcaccgc aaaaagaggt gactggtgtg gcttctggag
     4201 taacgaggat gtatcggtgt ggtggaaccg tctgtgtgac aacgtactgc cggaaaaaac
     4261 catgccgttt gacctgctga cggttctgcc gactcgcctg gatgttgaag tgaatggctt
     4321 taacggtggt gttctgaacg gtgttccttc tgcatatcac tggtatacgg aacggtatgg
     4381 cgtgaagtgg cctgtggggt atgaggtgaa tatcagtagt cagggagaca acttcattca
     4441 ggttgatttc gacacgccgt ggtgtcagcc ggaaagcgac gttattgcag aattaagccg
     4501 ccgtttcagc tgcacgctgg agcactggta tgccgaacag ggctgtgatt tctgtggctg
     4561 gcagttgtat gagcgcggag agctcgttga tgtgctgtgg ggggaactgg aatggtcttc
     4621 cccgacagat gacgatgagc tgccggaagt caccggacct gcgtggatag tcgacaatgt
     4681 ggcgcattat ggcggatgaa gtatgacgga gacgggcggg aaaccgcccg tttcttttcc
     4741 gacaaaggat gtcgccgtgc tcctcttttt actgcgccgg ctgacgcggc gcggcataaa
     4801 cgctgcctgt ggtcagtgtc ccgcgtccgg ccgggcacgg gacgggcgct tttaccgctc
     4861 ccggctggca tcggtgacag tgtacgccag cccgtcgccc ttttctgatg aacgcccttc
     4921 aagccgcttt cgcggcatat tctcgccgtc aaaaagacga cggctgcgat attccacggt
     4981 cggcctgacc cgttaccgga cgcggtgaac agcccacagg cagcggggaa cgggcaccac
     5041 aggggtgccc tcccggtgcc ctctgtaaga gaaggagttt cgtatgtccc gttttgtcct
     5101 cggtaactgc atcgatgtta tggcccgtat tcctgataac gccattgatt tcatcctcac
     5161 cgacccgcca tatctcgtcg gtttccgtga ccgttccggg cgcaccatcg ccggcgataa
     5221 aaccgatgag tggctgcaac cggcctgtaa tgaaatgttc cgcgtgctga aaaaagacgc
     5281 gttaatggtg agcttctacg gctggaaccg cgtcgatcgc tttatggccg cctggaaaaa
     5341 tgcgggattc agcgttgttg gtcacctggt cttcaccaaa aactacacat cgaaggccgc
     5401 atatgtgggc tatcgccacg aatgcgccta catcctggca aaaggccgtc cacgtctgcc
     5461 acaaaacccg ctgccggacg tgctgggctg gaaatattca ggcaatcatc atcacccgac
     5521 ggaaaagcct gttaccagcc tgcaaccgct gattgagagc ttcacacacc cgaacgcaat
     5581 tgtgctggac ccgtttgcag gcagcggctc aacctgcgtt gccgccctcc agtccggacg
     5641 ccggtatatc ggtatcgagc tgcttgagca gtatcaccgt gccggacagc aacgactggc
     5701 tgccgtacaa cgggccatgc agcagggggc cgcgaatgat gactggttta tgccggaggc
     5761 tgcgtaaatg aactatgccg gacacgaaaa acttcgcgcc gaagtggcgg aggtggccaa
     5821 tgccatgtgc gacctgcgta caaccatgaa tgagatggag cggcggtaca gctttaatgc
     5881 tgacaccctg ccggaacgtc tggtgcgtca gacgttgttt cgcgcaaacc gcctcctgat
     5941 ggaggcatat accgaaattc ttgaactgga tgcgtgcttc aaagattaag gaggagacga
     6001 aatgtacgga acatgcgaaa cgctctgccg tgagctggca gcaaagtatc cgggaaacac
     6061 gccgctgatg ctgcttatct ggtccccgga agagattcag gcactggccg acggtatgga
     6121 aatttctctg accgatcatg aaatcaggac tgtcctggcg cacctggagg acatcccgga
     6181 agaccagcgg atggagtccg gtatttcttc cgccgctgcg atggagatta tcagcaacgt
     6241 gagcgaaaac cgcctggtga ccgtctctgc tgaactgctg gcgtccctga ttcagaccgc
     6301 tgaacaggca ctgtggaaac gtgaatgggc cgcccgtgat aacggccttg ccgtcccgga
     6361 atgtgttacc cgccgtcagg cagtgattaa tcaggcccgc actctgctga aaaacaacac
     6421 acacgaaaac aactgatgtt atcgccgcct gccctgcggg cggcgataca gggagaacgc
     6481 ttaatcatga acaaaacgct gaatgcactg gtttgtcgtc acgcccgtaa cctgctgctg
     6541 gcgcagggct ggccggaaga gacggatgtt gaccagcgaa atccgaacta tccgggctgg
     6601 atcagcattt acgtgcggct ggatgcgccc cggctggcga cgttacttat caaccgtcac
     6661 ggcggcgtac tgccaccgct cctggcctcc gccattcaga gactgaccgg aaccggggcg
     6721 gaactggtac tgtccggcag tcagtggcag tcgctgccgg tacttccggc agacggaacg
     6781 caggtgtctt tcccgtatgc cggagaatgg ctgaccgaag acgaaatcag ggctgttctt
     6841 gatgcggtgc acgatgcggt acgaagcatc tgttaccagg tggcagaaga tgcgcgacgt
     6901 atccgtgcgg cgctgaccac caccggtcag acgttgctga cccgccagac gcgccgcttt
     6961 cgcctggtcg tgaaggaaag cgatcacccc tgctggctcg atgaagatga cgaaaacctg
     7021 cccgtggtgc tcgatgccat cgtgaaccgg ggagcacgtt tttcgtcggt ggaaatgtat
     7081 ctggtcagtg agtgtattga gcatatcctg tccagtgggc tggcctgcga tgtgctgcgt
     7141 ataccggatg aaccgccccg ccgctggttt gaccgtggtg ttctgcggga ggtggtccgg
     7201 gaagcccgga ccgaaatccg cagcatggcg gatgccctgg caaaaatccg gaaatgatga
     7261 cactggcggg gtaaatcccc gcctttttcc tgccggtccc cggacagagc acaaaggaag
     7321 tcgccgccgg ctccgcttta cccggccatg cagagcatgg ccttgtggtt tttcagttat
     7381 gtggcctcag cgtcgtgtgc gggctgtgcc gtgcctccat cttagccggg ctggcgttga
     7441 tgcaagggta cgcttcgccg ctgcggtcac ccggtccctc cttcccgtct gccgtgattt
     7501 tccggtcgtt cacccgctca gatttcgcgg tctcaccctc caactcccgc cagccgtgtg
     7561 cgtcaggctg cggcttccct tgcgcccctg catccccgcc tgttcgcccg gcttttatgg
     7621 aggcacggca ccgcccggtg tcgcggaata tgtgaacaac ggaggaaaac atcatgcaat
     7681 atgcgaaacc tgtcactctg aatgttgaag agtgcgaccg tctgtctttt ctgccttacc
     7741 tgtttggccg ggattttctg tatgccgaag cgtctgtgta cgcgctggcg aaaaaaatga
     7801 tgccggaata tgaaggcgga ttctggcact tcattcgcct gccggacggt ggcggttaca
     7861 tgatgccgga tggcgaccgt ttccacatgg tgaacggtgc aaactggttt gaccgtaccg
     7921 tgagtgctga tgccgcaggc atcatcctga cctcccttgt gattaaccgc cagttgtggc
     7981 tgtaccacga cagcggggat gcaggactga cccagcttta ccggatgcgc gatgcgcagt
     8041 tgtggcgtca catcgaattt caccctgaat gcaacgcgat ttacgcggct ctggactgat
     8101 taacggacgg ggcggcaatg ccgcccctgt aaaggagacg agaacatgta ctgtactgtt
     8161 aaagaaatta tccgcgaagt gctggataca gacgtgccgg acagtgaatg cgtttttgcc
     8221 gtggtgctga cccgtgggga tgtgcgccac atagctcagg actggagtct ggcagacgat
     8281 gagctggaaa ccgtcatgca gcggctggac gatgcctttg agcatggtgc ggatgtcagc
     8341 gttgttcacg acgttgttcg tgaactgatg gaagaaaagc gcgccagccg tcatgtgaca
     8401 gtcccggcgg tgatgctgga aaaagtgatg gcgctggcag gcagtgaaat gaagcgcctg
     8461 tatgccgtcg ggagtgag
//
LOCUS       DAZPJP010000172         8410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPJP010000172 DAZPJP010000000
VERSION     DAZPJP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.173"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            118..378
                     /gene="repA"
                     /locus_tag="ACHU9J_003434"
     CDS             118..378
                     /gene="repA"
                     /locus_tag="ACHU9J_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR1906895.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLTQLCKDSGLTQKEMIEHWILKEKAAMTDANRK"
     gene            complement(419..550)
                     /locus_tag="ACHU9J_003435"
     CDS             complement(419..550)
                     /locus_tag="ACHU9J_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFR1906896.1"
                     /translation="MFFVAKHRKIIGVFAFLAPVNPHQNQFPATLRRGQPQNSLNDQ"
     gene            611..685
                     /gene="tap"
                     /locus_tag="ACHU9J_003436"
     CDS             611..685
                     /gene="tap"
                     /locus_tag="ACHU9J_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR1906897.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            678..1535
                     /gene="repA"
                     /locus_tag="ACHU9J_003437"
     CDS             678..1535
                     /gene="repA"
                     /locus_tag="ACHU9J_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR1906898.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSVLFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2448..2585
                     /locus_tag="ACHU9J_003438"
                     /pseudo
     CDS             2448..2585
                     /locus_tag="ACHU9J_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            2605..2868
                     /locus_tag="ACHU9J_003439"
     CDS             2605..2868
                     /locus_tag="ACHU9J_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFR1906899.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSVQLRQQLQGRYQELRNEL"
     gene            2858..3157
                     /locus_tag="ACHU9J_003440"
     CDS             2858..3157
                     /locus_tag="ACHU9J_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1906900.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDAGNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDKARRVNILAVVGQRQNPRFIL"
     gene            3515..3790
                     /locus_tag="ACHU9J_003441"
     CDS             3515..3790
                     /locus_tag="ACHU9J_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFR1906901.1"
                     /translation="MKYSKPISSPSCFGERISTELEFVRKICRAEGETEADDDKLVDE
                     FRRLAEHPGGSDLIYYPRDDREDSHEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(3868..5055)
                     /locus_tag="ACHU9J_003442"
     CDS             complement(3868..5055)
                     /locus_tag="ACHU9J_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFR1906902.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(5112..5873)
                     /locus_tag="ACHU9J_003443"
     CDS             complement(5112..5873)
                     /locus_tag="ACHU9J_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1906903.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVAYVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWRAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            complement(5873..6910)
                     /locus_tag="ACHU9J_003444"
     CDS             complement(5873..6910)
                     /locus_tag="ACHU9J_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFR1906904.1"
                     /translation="MSVAAIETRRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGAGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTSASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            complement(6910..7908)
                     /locus_tag="ACHU9J_003445"
     CDS             complement(6910..7908)
                     /locus_tag="ACHU9J_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1906905.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGMCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNVEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIITANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            complement(8058..8216)
                     /locus_tag="ACHU9J_003446"
                     /pseudo
     CDS             complement(8058..8216)
                     /locus_tag="ACHU9J_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(8259..>8410)
                     /locus_tag="ACHU9J_003447"
     CDS             complement(8259..>8410)
                     /locus_tag="ACHU9J_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1906906.1"
                     /translation="RPPVLNEEQKQKVLERIKSGASISAIAREFRTTRQTILRVRAKQ
                     QTVTV"
BASE COUNT         2028 a         2324 c         2329 g         1729 t
ORIGIN      
        1 aatccccgtt gatttgtgcg ccaacacaga tcttcgtcac aattctcaag tcgctgattt
       61 caaaaaactg tagtatcctc tgcgaaacga tccctgtttg agaattgagg aggcgagatg
      121 tcgcagatcg aaaatgcagt aacttcctca ccgaaacgca tttacagaaa aggtaatccg
      181 ttaaccggag ccgagaaaca acgcatatct gtttcccgga aaaaggggac acataaagcg
      241 attaatgttt tcatccagag tgaactcaaa gatgatttga cacagctttg taaggattcg
      301 ggcttaactc aaaaagaaat gatagagcac tggattttga aagaaaaagc tgcgatgact
      361 gatgcgaata gaaagtaact aagttacttt cttgaacatt tgaattgcgg atgctagatt
      421 actgatcgtt taaggaattt tgtggctggc cacgccgtaa ggtggcaggg aactggttct
      481 gatgtggatt tacaggagcc agaaaagcaa aaaccccgat aatctttcta tgtttggcga
      541 cgaagaagat taccggggct aacaagaaac cgtatagcca acaattcagc tatgcgggga
      601 gtatagtcat atgcccggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat
      661 tgtaagtgca ggatggtgtg actgatcttc accaaacgta ttaccgccag gtaaagaacc
      721 cgaatccggt gtttacaccc cgtaaaggtg ccggaacgct gaagttctgc gaaaaactga
      781 tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcgcatgccc
      841 gttcgcgtgg tttgcgtcgg cgcatgccac cggtgctgcg tcgacgggct attgatgcgc
      901 tgctgcaggg actctgtttt cactatgatc cgctggccaa ccgcgtccag tgctccatca
      961 ccacgctggc cattgagtgc ggactggcga cagagtccgg tgcaggaaaa ctctccatca
     1021 cccgtgccac ccgtgccctg acgttcctgt cagagctggg actgattacc taccagacgg
     1081 aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca tctgtactgt
     1141 ttgctgccct cgatgtatca gaggaggcag tggccgccgc gcgccgcagc cgtgtggaat
     1201 gggaaaacag acaacgcaaa aagcaggggc tggataccct gggtatggat gaactgatag
     1261 cgaaagcctg gcgttttgtc cgtgagcgtt tccgcagtta ccagacagag cttaagtccc
     1321 ggggaataaa gcgtgcccgt gcgcgtcgtg atgcgggcag ggaacgtcag gatatcgtca
     1381 ccctggtgaa acgacagctg acgcgtgaaa tcgcggaagg gcgcttcact gccagtcgtg
     1441 aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc
     1501 gcaattacag ccggctggcc acagcttctc cctgaaagtg acctcctcag aataatccgg
     1561 cccgcgccgg aggcattcgc acgcctgaag cccgtcagtg cacaaaaaat cagcaccaca
     1621 tacaaaaaac aacctcacca tccaccttct ggtgcatccg gttccccctg tttttgagac
     1681 aaaacacgcc tcacagacgg ggaattttgc ttatccacat taaactgcaa gggacttccc
     1741 gataaggtta caaccgttca tctcataaag cgccagccgc cagtcttaca gggtgcaatg
     1801 tatcttttaa acacctgttt atatctcctt taaactactt aattacattc atttaaaaag
     1861 aaaacttatt cagtgcctgt cctgtggaca gacagatatg cacctcccac cgcaagcggc
     1921 gggccccgac cggagccact ttagttacaa cacacaaaca caaccaccag aaaaaactcc
     1981 ggtccggcgc agaactgaaa ccacaaagcc cctccctcat aactgaaaag cgccgccgcc
     2041 ccgcccttcg ggcgggaaca acatcgcttt taattatgaa tgttgtaact atactccgtc
     2101 accgctgtca gtcttctcgc tggaagtatt gagtacacgc tcgtaagcgg ccctgacggc
     2161 ccgctaacgc ggagatacgc cccgacttcg ggtaaaccct cgtcgggacc actccgaccg
     2221 cgcacagaag ctctatcatg gctgaaaacg ggtatggctt agcagggctg gggatgggta
     2281 aggtgaaatc tatcaatcag taccggctta cgccgggctt cggcggtttt actccggtat
     2341 cacatgtaac cacagcgtgc cgccttacat gccgctggcg cggcataagc atatgttggt
     2401 ggctgtgtct gaaaggttat atactctgca taaaacctga caacacgcag attgccaggc
     2461 aaatgagcat tatgcatccg gttatcgcct gcctgtgaaa caatatcacc ggggcgaata
     2521 tcgatactct gactcgttat acccatgcgt gcggcatcaa aaaacctgca attgcccttt
     2581 attgaatgaa agggggagtc aattatgaat agcaccacac attacgaaaa cgccaatttc
     2641 ctgcgcgagc tggccgaaag cctgcctcgt atttttccag aaggcagtac agacaagtct
     2701 gcactattgc aacgcctcgc taatgaagaa ctggcacggg cggaatacga cgaacagatc
     2761 cgagcgaaag ttgccgctgc tcgcgccgat aagcggccgg gaatgagttc cgtgcaactg
     2821 cgccagcaac tacagggacg ttatcaggaa ctgcgcaatg agttatgaaa tagaatggcg
     2881 accaggtgcg attgaggata taacggcgtt gtttgaatat ctggccgaga atgcatctct
     2941 ttgggatgca gggaacgtga cagagcgtct gatcactgca accgatcgtc tggcagatta
     3001 ccccaggctg tatgaaacag atgaacgcta tggcgaaggg gttcgccgta ttagcttact
     3061 tgggcagcat gtactttatg aagtgaatga taaggcccgt cgtgtcaata tcctggcggt
     3121 tgtaggccag cgtcagaatc cccgattcat tctttgagtg cccggcttga tgcgggctta
     3181 ccaggaatca cggtttggga gccgcctgga gaaatgaaat tttcccatgc taagagtatt
     3241 gagttgaact agtagaattg acaattcaaa aactgagaac atgaagaaac cctatacgtc
     3301 tgtcatactc gctcgtaagc ggccctgaag gctcgcttac tcgacatccg ttagttataa
     3361 cgtcagaaga attatcgagc tttactcaag atcttcttcg tcaatctcca gccatccacg
     3421 ataagctgac tctgtatctt tcacatcgtt ttgagtcata tcagcattgc gtagaaaatt
     3481 aaaagctgca gcacaaaatt gatcttccag gtatttgaaa tactccaaac ctatatccag
     3541 ccccagctgc tttggcgaga gaatatcaac tgaactggag ttcgtcagaa aaatctgccg
     3601 ggctgaaggt gaaactgagg ctgatgacga taaattagtg gatgaatttc gtcgattagc
     3661 tgaacaccct ggtggttctg atcttattta ttatccaagg gatgaccgtg aggacagtca
     3721 tgaaggtatc gtaaaagaag tgaaggaatg gcgagccaaa aatggcaagc cggggtttaa
     3781 acagggctga ggatctttgc taaaggatta gctgaaaata tggatactag ctgttcactt
     3841 ccagagcggg ctgttacccg ctctgcgtca tgttgccgcc agcgggcgac agatcgcccg
     3901 cagggcgacg gcgctgcaca gcggaaacag cgccagcaga agccagggat aaaccgcctg
     3961 cggcgacgga atgagcgcct ggtcaagtaa atgaccgagc agtaaattcc cggctaacac
     4021 cgcgcaccct cctgcagtgg cgagcgctcc gtagtgggca cccagcgtcg actcctcggc
     4081 gaacagcggg atcagatcct tggctgcggg cactaacagc atctggccga gggtgagcag
     4141 cgtgacaaaa ccagccgccg gcattaaccg cagccaactt tcggcgggtg gggcggcggc
     4201 gaataacgcc acgctggcaa aggaagccga cagcagcaga aagcccaccg gcaggatgcg
     4261 cacggcgccc attcgccggg cgaagcgggc gagagggagc tggagggtga taatcaacag
     4321 cgaggccagc ataaagagcg gcgccagatc ctgctcgcgg ccgccggaac gttggatctc
     4381 caccggcagg gcgaggtaca gttggttgta gctcaacagc catgagctgt aggcgagaat
     4441 aaacgcgaca aagcgcggct ggcgcaaagg cgtccaccat gggactcgtc tgcgcgttgt
     4501 tgtcgtgtgc ccgtcggcgg gcagaccgaa aaagagcacc gccagcgcca gaagaaatat
     4561 tcccgcgccg gcgagggcga tatgccgaaa gccgatcccg ctcagcacgc ctccggccac
     4621 cgggccgatc accgctccca gttcaccgca taccgcaaac agcgcaaacc actcggcgcg
     4681 gctgcgtttc ccgttggcct ggctgtgggt accggcccgc gccagcaggg cctctatcga
     4741 cggggagaac agggcgccgc cgacgccggt caggcaggcg ccaaggataa tgggccacag
     4801 cgacgccccg cacgccagca ggagaaaacc ggcgacgcga acgacgcagc cggcgagaat
     4861 gatcgcctta gcgccatagc ggtcggccag cgtcccgccg aggataaaca tcccctgttg
     4921 ggagaaggta cgcagcccga ggatcaggcc gataagtccg cctgagagca gcatgtcgtc
     4981 gcgcaggaag agggcgagga aaggaacgac cgcgtaaaag ccaatattaa aaacgaactg
     5041 gcttcccagt aacaccggag gccagagggt tgccgcaggg cgcagggaga aaagaggcat
     5101 atccgcccaa cctaaacgat gaaatgaatg tgctttttgc cgtggtaagg ggagatctcg
     5161 attttggttt tcacccggaa aacgtcccac agcagcgctt cggacaaaat ctcctccggc
     5221 gtcccgctgg cgaggatctg cccctgctgc atcacgatca gcgaatcgca gaacatcgcg
     5281 gcatggttaa gatcgtgaat ggccacaatg ctggttaccg gcagctcgct gatcaactgc
     5341 attaactgca tctgatggtg tatatccaga tggttggtcg gctcatccag caggatttcg
     5401 ctcgggctct gcgccagcgc gcgggcgata tgcacccgct gccgctcgcc gccggagagg
     5461 cttaaccatc cctgttcgct tttctccagc atcgctaccc gctgcagcgc ggcggcaatc
     5521 gcttcgtcat cctgagctct ccagtttgag aacggagagt ggtggggaat gcgtcccagg
     5581 cgcacgacgt cgcgcacccg catattggcc tcggtcatgc cgtgttgctc gacgtaagcc
     5641 acgcggcggg cgagctgctt tttcgccatc cgggcgatat cctggccgtc gagggtgacg
     5701 cgacctgcat ccgggcggcg caggcccgcc agaacgcgca gcagcgagga tttgccgcag
     5761 ccgttgggcc ccagcagtcc gaccgtttcg ccgcgcggca cccgcagcga gacattattg
     5821 acgatgacct ttttgcctgc cttccaggtg atattttcag cgcaaatact catcacttat
     5881 tccttgagcg gtagataatc acggcaaaga aaggcacccc aaccagggcg gttactaccc
     5941 caacgggcag actttgcggg gcaatcagca ggcgcgacgc aatgtccgcc agtaccatca
     6001 atatcgcccc tgccagcgcg ctggcgatca gcaaagttcg gtgcagtggg ccaaagaaga
     6061 aacgcatcac gtgcggcacc accaggccaa caaagccgat tgagccggcc atgctgacga
     6121 tggtggcggt gatcagcgcc gcagtggtga agaggatcag gcgcacgcgc ggcacagcaa
     6181 tccccagcga ggcggcggca tcgtcgccga aggtgaacgc gtccagcgcc cgggcatacc
     6241 acaggcagac cgccagaccc gcgagcacca ccacgatgac caactggaat tcgggccagc
     6301 ggacgccgct gaagctgccc agcagccaga acatcacatc gcgcgcctgc tgtgcgctgg
     6361 cggaggtgct gatggtatag gccgtgatgg cgttaaataa ctgcgaggcg gcgacgcccg
     6421 caagaatcgt acgctcattg ccgccgcgcg cgccgttagt cagcaaggcg acaaaggcga
     6481 aggcggcgaa ggccccggca aaggcgcctg ccgagaggga aattgcgcca gcgccaaggc
     6541 ccagcaccac gatcgacacc gcgccggtgg aggccccggc ggagacgccg aggacgtagg
     6601 gttcggcgag ggcattcttc agcaagcttt gcagcacgac accgcagata gcgaggccgg
     6661 cgccgcagca ggcggcgacc agcgcgcggc tgaggcggaa atcccagata acgctctcat
     6721 agatacggtt gagcggctca gcggttaagc cggttctgtt gctgatagca taaaagacat
     6781 tctgcagcgg gatggccagc tcgccaacgc tgacgcccac agcgatcacc agcgccagca
     6841 cgatcgctgc cagtacacac cagccggtga gcagtatgct gcggcgcgtc tcaatcgcgg
     6901 cgacgctcat cagttcagcc ccatttttct cagctgctcg cccacctgct ccgcgccgta
     6961 aagcgtgcga atggtcggat tcatcgcctg accgtccatg accacgatat ggcctttttt
     7021 caccgcctcc agctggctga cggcgggatc gcttttgagg aatttgatct tttcctcggc
     7081 cttatccagc gcccagcggt tacgatccag gctggcgacc acgatcacat ccgggttggc
     7141 ggtaataatg ctctcccagc tcaccgtggg ccactcggtt tcggaggtaa tcgcattatg
     7201 tccgcccagc acgctggcga taaatccgga ggcgctgttt ttgccgccga cataggcatc
     7261 ggccgaaggt gaggcgctgg agaaccagaa gacgaaggag agatcctttt tgcttttacc
     7321 aaactcctgg cgcaggtcgg cttcacgttt tttgaaatcg gcaatcagcg cctgaccacg
     7381 atcttcgacg ttgaaaatgc gggaaaaatc gctaatttcc tgatacagcc aggtcatatc
     7441 ccacagcttt tgccggctgc cgtacatatc gccggtggcc tttttggtgg cgcacatccc
     7501 cggtgagacg tagctgttca cgccgacggt ggcgagatct tcacgcctgg cgactttgct
     7561 ttccggcccc agcagcagcg gcaactgggc gggaacgaaa tcaggatttt gcgccagcac
     7621 cgattccagg ctcggaattt ccaccgtcaa ggttttaatc ttcgcgtttt gctcagccag
     7681 ctgcggtaaa acgctggtcg gccagaaggc gctggcgacc acctgctttt gcagcccgag
     7741 cagcagcaga atttcgaccg tattttgccc cagcgcgacc acgcgctccg gcggccgggt
     7801 gaaggtctcc tgatagccgc agttttcaat cgtcaggggg taggttgttg ccagcgcgga
     7861 actgacggcg gtgaacatca agcctaacgc gcaaaggacc ttcttcatta aacgctatcc
     7921 ttgtaaacag gcgagtggat gaccagcaaa tgggagtaat gcaaatgaaa ctcattattg
     7981 gggcggagtt ttatacaatg ccatatttac agtgtcaatg tgttcttgcc cccttatttc
     8041 cagacagggg ttgtatctta agttaacgtt acccactgac gtcgatattc tcgcggagag
     8101 cgataaccca acgcactgtg cggatggttt tcattgtaat gttcgatcgc cactgcaaga
     8161 ttatgcaatg ccgttcttac attcggtttc ggcatgaacg cgatgtagtc ttccttgggg
     8221 tccgcttgga aaacggaatc ccacgctaag actattttct atacagtaac agtttgctgt
     8281 ttcgctctaa ctctcagaat tgtttgccgt gtggttcgga attccctggc aatagcactt
     8341 atacttgccc cagacttaat gcgttcgagt accttctgtt tttgttcttc atttaatacc
     8401 ggtgggcgac
//
LOCUS       DAZPJP010000173         8349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPJP010000173 DAZPJP010000000
VERSION     DAZPJP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.174"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHU9J_003448"
     CDS             complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHU9J_003448"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1906907.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHU9J_003449"
     CDS             complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHU9J_003449"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1906908.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHU9J_003450"
     CDS             complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHU9J_003450"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1906909.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4352..5467
                     /gene="malK"
                     /locus_tag="ACHU9J_003451"
     CDS             4352..5467
                     /gene="malK"
                     /locus_tag="ACHU9J_003451"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1906910.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLNIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5539..6879
                     /gene="lamB"
                     /locus_tag="ACHU9J_003452"
     CDS             5539..6879
                     /gene="lamB"
                     /locus_tag="ACHU9J_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1906911.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7031..7951
                     /gene="malM"
                     /locus_tag="ACHU9J_003453"
     CDS             7031..7951
                     /gene="malM"
                     /locus_tag="ACHU9J_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1906912.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8180..>8349
                     /locus_tag="ACHU9J_003454"
     CDS             8180..>8349
                     /locus_tag="ACHU9J_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906913.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIK"
BASE COUNT         2080 a         2152 c         2150 g         1967 t
ORIGIN      
        1 aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa ataataagct ctatgatgga
       61 aatattaacc ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc
      121 gccgaataac agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg
      181 cagaacaaca tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt
      241 gagccagcaa gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt
      301 caccccacag gtagttttgc gggttgaggt attgctgcat ccccacggcc agggtgtagc
      361 tgtttacgtc acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg
      421 acaggatgaa taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg
      481 cctgccacgg tgtcgcacca tccagcgcag ccgcttcttc cagcgaacta tcgatggttt
      541 cgaaatagcc tttgatggtc cagacatgca gcgcaatccc acccagatac gcgaaaatta
      601 cgccgccgtg agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg
      661 catacaacgc gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca
      721 gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga
      781 gcgcgacaat gccaatcgcg gaaatcccgg cgacctttac cgagttccat agccacagca
      841 gtaccgggaa tggcggtggc gtaatgcgac catcagcctg ttccacgcta aaacctaaag
      901 ccagtttcca gtgatcccag gagatttgct ccgggatcag gctgccgatc gcaaagttcc
      961 cctggcgcag tgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa
     1021 gtagcagtag caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg
     1081 ccatttttgt tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc
     1141 cagcgcaccc accagcagga agagcagcgt ggcaattgct gcagccagac cgaagtcctg
     1201 acccccgccg ccttcaaaag cgatacggta ggtgtagtta acaagcaggt cggtataacc
     1261 ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg gttaacagtt gaatcagcac
     1321 gaagttgtta aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag
     1381 cagcggcagc gtaatcttaa agaagttctg gaacggacct gcgccatcca ttgctgaggc
     1441 ttcatacaaa tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg
     1501 ataacccagc caggtattga cgataattag catcgtgcgg gcggtggtcg gatcgctgaa
     1561 ccaggcgggc ttcacgccaa acagcgcgct caacatcatg ttgatttcac cgaagctctg
     1621 gttaaacaac cctttgaaaa tcaagattga aatgaatgat ggcaccgcgt agggcagaat
     1681 cagcagaacg cgatagaccg ctttgccgcg caatgcttcc cactgcacca gacatgccag
     1741 aaccatgccg accgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac
     1801 gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt
     1861 ccagccagta gtcacggtgt aaccggggct tagcttttca tcaccccagt tgccgtcggc
     1921 ggtaatggac tggtaaaagc caatttggtt attcggacga tatttcacgc cgctctgatt
     1981 attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg
     2041 cagggagctc atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc
     2101 ctgacgattc tgggtaatca cgcgtagatt cgcgcgttcg ccttcgggct gggcggtcgt
     2161 ttctttcagt tgcagttttt gttcgccgcc aaatttaaac gcgtcggaga ggtaattttt
     2221 gccgatttcg ccgtcactga gcgccagttg ccactcatcg cccgccgggt aaagaccaaa
     2281 gttataggtt ttgcctgctt gccaggagcg atctaacaac acttcctgcg cacattcaaa
     2341 agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg
     2401 gaagaggacg aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc
     2461 tttacgattg gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa
     2521 caggtattcc ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag
     2581 acctagcact gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc
     2641 catggggttc ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact
     2701 gcctgatgcg acactgatgc gtcttatcag gcctacatgc gtttcagttt tgtaggccgg
     2761 acaaggcgtt cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc
     2821 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
     2881 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
     2941 gttttccatg gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag
     3001 cgccactgca cccagcggtt tgtctttatt gaccgcttcc agaccttcgt cagtcagcag
     3061 atagttttcg aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggc
     3121 gctcagcacg ccaacgaacg gtttagatgg ttgacccttg aaggtcggca gtaccgttac
     3181 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
     3241 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
     3301 gtgtttgttt ttaataaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
     3361 cacggccacg tctttaatat cgtacttgcc gttttcatac ttgaacgcat aacccccgtc
     3421 agcagcaatc agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt
     3481 acctttcgct ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggtgggtt
     3541 cggcagcaga tctttgttat aaatcagcga taacgcttca acagcgatcg ggtaagcaat
     3601 cagcttgccg ttgtaacgta cggcatccca ggtaaacgga tacagcttgt cctggaacgc
     3661 tttgtccggg gtgatttcag ccaacaggcc agattgagcg tagccaccaa aacggtcgtg
     3721 cgcccagaag ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc
     3781 cagtttatcc ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc
     3841 gacttcagcg agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc
     3901 ttcgattttg gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag
     3961 gatgcgtgca cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga
     4021 aaacacctaa acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc
     4081 tacgccccac cgttgctttg tgtgatctct gttacagaat tggcggtaat gtggagatgc
     4141 gcacataaaa tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg
     4201 tttagttcac agaagccgtg ttctcatcct cccgcctcct cccccataaa aaagccaggg
     4261 ggtggaggat ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt
     4321 cgatgacagg ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac
     4381 gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat ctcaatatcc atgaaggtga
     4441 attcgtggtg tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc
     4501 cgggcttgag acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac
     4561 tccgccagca gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct
     4621 gtcagtagca gaaaacatgt catttggcct gaaactggct ggcgcaaaaa aagaggtgat
     4681 taaccaacgc gtcaaccagg tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa
     4741 accgaaagcg ctctccggtg gtcagcgtca gcgtgtggcg attggccgta cgctggtggc
     4801 cgagccaagc gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt
     4861 gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt
     4921 cacccacgat caggtcgaag cgatgacgct ggccgacaaa atcgtggtgc tggacgccgg
     4981 tcgcgtggcg caggttggga aaccgctgga gctgtaccac tatccggcag accgttttgt
     5041 cgccggattt atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc
     5101 aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt cagcaagtct ggctgccagt
     5161 tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct
     5221 actgccgagt gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact
     5281 cggcaacgaa acccaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg
     5341 ccagaacgac gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga
     5401 gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg
     5461 cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc
     5521 aatgactcag gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc
     5581 gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc
     5641 ggtattggct ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa
     5701 agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa
     5761 gtgtggaaag agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg
     5821 caacagaatg actgggaagc taccgatccg gccttccgtg aagcaaacgt gcagggtaaa
     5881 aacctgatcg aatggctgcc aggttccacc atctgggcag gtaagcgctt ctaccaacgt
     5941 catgacgttc atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg
     6001 gaaaacatcg atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagct
     6061 ggtggttctt cctcttttgc cagcaacaat atttatgact ataccaacga aaccgcgaac
     6121 gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc cgggcggcac attagaactg
     6181 ggtgtcgact acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg
     6241 aaagacggct ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag
     6301 tttgttgttc agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt
     6361 tctggcgtcg cgtttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg
     6421 ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca actgggacat gatgtacgtg
     6481 ggtatgtacc aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgtcggt
     6541 attcgcccga tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac
     6601 aacgtcgaat cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa
     6661 caatggcagg ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac
     6721 gccaagtggg atgagaaatg gggttacgac tacaacggcg atagcaaggt taacccgaac
     6781 tacggcaaag ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac
     6841 gagtggacct tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata
     6901 aggcgtttac gccgcatccg gcataaaaac aggttgtcat tatctgaaag gggcgaaagc
     6961 ccctctgatt atcgggttta gcgcgctatt gcctggctac cgctgagctc cagattttga
     7021 ggtgaaaaca atgaaaatga ataaaagtct catcgccctc tgtttatcag cagggttact
     7081 ggcaagcgcg cctggaatta gccttgccga tgttaactac gtaccgcaaa acaccagcga
     7141 cgcgccagcc attccatctg ccgcgctgca acaactcacc tggacaccgg tcgatcaatc
     7201 taaaacccag accacccaac tggcgaccgg cggccaacaa ctgaacgttc ccggcatcag
     7261 tggtccggtt gctgcgtaca gcgtcccggc aaacattggc gaactgaccc tgacgctgac
     7321 cagcgaagtg aacaaacaaa ccagcgtttt tgcgccgaac gtgctgattc ttgatcagaa
     7381 catgacccca tcagccttct tccccagcag ttatttcacc taccaggaac caggcgtgat
     7441 gagtgcagat cggctggaag gcgttatgcg cctgacaccg gcgttggggc agcaaaaact
     7501 ttatgttctg gtctttacca cggaaaaaga tctccagcag acgacccaac tgctcgaccc
     7561 ggctaaagcc tatgccaaag gcgtcggtaa ctcgatcccg gatattcccg atccggttgc
     7621 tcgtcatacc agcgatggct tactgaaact gaaagtcaaa acgaactcca gctccagcgt
     7681 gttggtagga ccactatttg gttcttccgc tccagctccg gttacggtag gcaacacggc
     7741 ggcaccagct gtggctgcac ctgctccggt gccggtgaag aaaagcgagc cgatgctcaa
     7801 cgacacggaa agttatttta ataccgcgat caaaaacgct gtcgcgaaag gtgatgttga
     7861 taaggcgtta aaactgcttg atgaagctga acgcctggga tcgacatctg cccgttccac
     7921 ctttatcagc agtgtaaaag gcaaggggta attacgcccc acagtgctga ttttgcaaca
     7981 actggtgcgt ctcctggcgc accttttttt atgcttcctt cctgggatat gagcgatttt
     8041 ttatagtact tcacttcttc ttcactaaga atgtccatta tctcaatgcc ttatcagaga
     8101 ttcttttcct ttcgccggta gtgtctggac gctcaggcta cttttccagg ttattttatt
     8161 tctgtattac agaggtttta tgataggtca tatcacaaat tctggcggca ataactcttt
     8221 gatgaaacat gatgtggttc aaggaaataa tatagtagat cttgatttac tacgtaattt
     8281 caatggggtg acaggtttaa acagagataa ctttatttat atcagtgata tatttttaaa
     8341 tataaaaca
//
LOCUS       DAZPJP010000174         8298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPJP010000174 DAZPJP010000000
VERSION     DAZPJP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.175"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHU9J_003455"
     CDS             complement(42..110)
                     /gene="ykiE"
                     /locus_tag="ACHU9J_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1906914.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="ACHU9J_003456"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="ACHU9J_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1906915.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="ACHU9J_003457"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="ACHU9J_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1906916.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="ACHU9J_003458"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="ACHU9J_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1906917.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="ACHU9J_003459"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="ACHU9J_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1906918.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="ACHU9J_003460"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="ACHU9J_003460"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1906919.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="ACHU9J_003461"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="ACHU9J_003461"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1906920.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="ACHU9J_003462"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="ACHU9J_003462"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1906921.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1802 a         2379 c         2316 g         1801 t
ORIGIN      
        1 cagcattacc gaaatcccta tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagaggc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataactattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cattcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct ctgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtg ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatat gcgttttgat gcgcatcagc
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgccgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaacg ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga acccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatgtgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatggg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggatgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggtg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca gggggcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca cggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgtcgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggcgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccgttgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctgagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggct ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tgctgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcactcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgcccg caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccctataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcggccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcaattac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcaatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aatcaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccag tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtgt
//
LOCUS       DAZPJP010000175         8259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPJP010000175 DAZPJP010000000
VERSION     DAZPJP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.176"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /locus_tag="ACHU9J_003463"
     CDS             complement(<1..297)
                     /locus_tag="ACHU9J_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFR1906922.1"
                     /translation="MSTNLAFRYASVETFFKMIENQELWFTDLRNMNDPEEYSAGYEI
                     VKRIINEEFPDCIRLLEEVSPNKIDENFKILICCFCSDGDRSNMWSEYGDNGKGV"
     gene            complement(420..3122)
                     /gene="nikB"
                     /locus_tag="ACHU9J_003464"
     CDS             complement(420..3122)
                     /gene="nikB"
                     /locus_tag="ACHU9J_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFR1906923.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPKKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRNRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKLWESPSPMR"
     gene            complement(3133..3465)
                     /gene="nikA"
                     /locus_tag="ACHU9J_003465"
     CDS             complement(3133..3465)
                     /gene="nikA"
                     /locus_tag="ACHU9J_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HFR1906924.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            3699..4034
                     /locus_tag="ACHU9J_003466"
     CDS             3699..4034
                     /locus_tag="ACHU9J_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR1906925.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(4120..4968)
                     /locus_tag="ACHU9J_003467"
     CDS             complement(4120..4968)
                     /locus_tag="ACHU9J_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR1906926.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMCDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            5317..5515
                     /locus_tag="ACHU9J_003468"
                     /pseudo
     CDS             5317..5515
                     /locus_tag="ACHU9J_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161114.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5549..5800
                     /locus_tag="ACHU9J_003469"
     CDS             5549..5800
                     /locus_tag="ACHU9J_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR1906927.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(5831..6076)
                     /locus_tag="ACHU9J_003470"
     CDS             complement(5831..6076)
                     /locus_tag="ACHU9J_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906928.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     ALEIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(6076..6990)
                     /locus_tag="ACHU9J_003471"
     CDS             complement(6076..6990)
                     /locus_tag="ACHU9J_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1906929.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGALEKALAIAC
                     QLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(7055..7321)
                     /locus_tag="ACHU9J_003472"
     CDS             complement(7055..7321)
                     /locus_tag="ACHU9J_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906930.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(7415..7849)
                     /locus_tag="ACHU9J_003473"
     CDS             complement(7415..7849)
                     /locus_tag="ACHU9J_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR1906931.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT         1955 a         2223 c         2025 g         2056 t
ORIGIN      
        1 aacgcctttg ccattatcac catactcgct ccacatgttg gaacgatctc catcagaaca
       61 gaaacaacaa attaatatct tgaaattttc gtctatctta ttaggtgata cttcttctaa
      121 tagtctaatg caatctggaa attcctcatt tattattctt ttgactattt catagccagc
      181 cgagtattct tccggatcat tcatattccg aagatcggta aaccataatt cttggttttc
      241 aatcatttta aaaaaagttt caacacttgc ataacgaaaa gcaagattag tgctcataaa
      301 gcatgattcc gtcaatagag ttacttaaca ttgcctaaca tatttatttt taccaccaca
      361 tccatattta atattggtga ttttttcatt ctataacgaa actaataaag cttcatctgt
      421 catctcatcg gagaaggcga ttcccagagt ttatcctgct ccggcggagg cagtgacgca
      481 tcatcattgc tgttcgccgc gatataatcg cggtagtagc gttcacttcc ttcacggtga
      541 tgatccacgt ccggtcgcgt aatcctgtag tcttcgtgac cggtacggcc tgtcacatta
      601 tgccaggcaa ccatttcagc aaagacctgg ttaaattgtt tgtcgtttcc ttctggttca
      661 aaacccatgc gtggatcacg cccgaataat accggtgcaa tcaaatccag tttatctgcc
      721 agcgcattgc ggtcatggtg attacggaaa acaaccctgt caccataatc cgtacagaca
      781 aactctcccg tccgacggtt acggaaacga acactcccgt tcttctgcag acgggcctcg
      841 agatcaccgg tattaccgta taccggcgtt ccgcccggtt cacaaagcac gacacagcgg
      901 tctgtcgtcg ttgtgcgtga cctgtcttta cggcgatcgc gataatccca gcctctcaac
      961 tgcgatacag cagccctgtc tccctgagca gcctgaattt cgacccactg acggtaagag
     1021 ggtggatacc atttcccgtc agcaataagt ttctgtcgct catccctgat gtcttctttc
     1081 agcctgatca atgcctgcat tcgctgaact tctgcaatgt gataatgcag cttacgtaat
     1141 gccggatcat cgtactgcgc acgaatgtgt gacttccgca gacggcatgc ctgatgaatt
     1201 tcccgacaac gttccccata acgcagatct ggtttacgcc attgttcacg ccatgccaga
     1261 tagcgggcac gcaggtcagc acgggcctca gctctggctt cacgtcgctg ccggcgcagc
     1321 attgggtcac gtttactgct gaccccgtac ctgtcactga cagccagctc cgggttatag
     1381 cgactttcag gttgcaccct gtcaaacaaa tctgctgggg cactcacaaa aggtccggcc
     1441 tgtggctctg cacgccctag tgtcagatca ggatgaatgc tgctggcctt caccggcgtt
     1501 tgctcatgat taaaggcatc aacgacaaca agcccgtgat gttgtttctg tagcatcagt
     1561 ccctgttgtg caaacaactc atgacaacgc tgccaggtaa actcaccctg tgtgatcatt
     1621 tcttcaatct gagggctaca atggctgatg ctgtatcccc acagactttc tttgtcagaa
     1681 aacagttcgc gtttgatagc cccctgcggc cccgtacctg tacgcatacg gtgccattct
     1741 ttatcggtcg caggacagct ctccatacga cgaccacgga agcgttctgt tggcgtaacc
     1801 tggcggaaaa tatctgttgg tacaggctgc cagccaccat cgagttgatt cacattgtcg
     1861 agcgtcagca gtgaccatac gctgtcagca cgaacgggag tctggttatg atcataaccg
     1921 tcacagataa ccaggtgacc atgctgaaca cgcatccata atcccgcttc agccagtgtg
     1981 cggtgaatag cctggcagtt ttccagccgt ccttcccgtg ccatttccgg cagacgttct
     2041 ccaagatgac gacaggcata ctgctgcaga ctttctgttt cagcgatttt tttcgggcga
     2101 acatcagctg caatgaagtc cggattatag cgtcctggtg cttccacctg gctgaaaatg
     2161 tcttttggca ctggcgtgta gtcacccatt tttttcatga gtttttctgc acaccaggag
     2221 ggaccaaacg agtcaagctg tacaccttcc ctgttgcgat cccagccatc catcaccaga
     2281 aattttccgt ccatctgaga caggtacaga ccatcttcag caagacgacg atgcagggat
     2341 aaccagtcat gtacaggttc actgcgtaaa ccagcgaccg ctgtctgcgc aacatactcg
     2401 cgaaaagttt gtttttttcc gcgtgtccaa gcgttctggc gatcacgttc aacggcagtt
     2461 ttgcgaacga tacgatttcc cggcgcatgg acccagcaac cgttatccgg agcaaaacca
     2521 tgcttcagtt caagctcacg gcaggcacga ctgagttttt cctgactcca tgataaccgg
     2581 ttcagataac ccgtttcagg gtgtacccgg ttaacagcca catggacgtg cagattatct
     2641 gtatcagtat gcacggcaga gacatactga tggtcggcaa ggccgagtga tttcagcgta
     2701 tgacgtacac tgtcataaat ctgttccgga cgcgggcttt catgtgactg ccaggaaagg
     2761 atgtagtgaa aaacaggatc agtgtcatct tttgcatagt gtgcctgccg ggcaatgtac
     2821 tccatatctg cagcggcagt ttcaagagaa gtacagttat gaaagcaggt gacaccataa
     2881 aagttgaccc attcgcagcc gtccttcatg acatcaacca gcgccacgaa tgactcattt
     2941 cgaagacgtg tcgcataatc aacaaggcga ctgaaacggc ttcggtgcgg ttgttcagcc
     3001 tgcgaagaag aagataaatc cagttcctca tcggtcatgt catcccggac agagacgtag
     3061 gataccaggt cttcgaacga agacttgccg tcccttctct ttttcggaat gactgcattc
     3121 accggagaat cctcaacctt ccattaactg tttacgcaat gtatttgtaa gttcggttat
     3181 ggcaacaagc acttctgcgt attccttgtc accggttctt tttccctgaa caaacagatg
     3241 cttctgcatc cttcccagtc tcattaactc cttcagaaaa gcatcatctg tacgggagtt
     3301 aattcgctta ttcagtgctg catttcggat gtaagcagat acagtaagtc cggaatcttc
     3361 agcttttttc ctgatggttt catcctcaac aggagagaat ctgagtgtcc tgacaactgt
     3421 cttttgccgt acctcacttt tctttcttac cgcactatca ctcacttcag gctccttacg
     3481 gggtgtcggg gcgaagccct gaccagatgg caattgtaat agcgtcgcgt gtgacggtat
     3541 tacaattgca catcctgtcc cgtttttcag gccattatag cctgttattt ttgtgcatca
     3601 tagtcttatg cagacggcag aggcagggcg cagccctgcc aggagtcagt ttttgatgat
     3661 aaaatcaggc ttcaacatga aacggagaga aagtgccaat ggcttctaaa aaattttact
     3721 cagatgatga catccagctg gcaaaagctg ctttatcgga attgccagat ctcaccgctc
     3781 aacggaaaac attgcgtgat tttcttgatg ccattcgtga tgacattatt gtcctggtaa
     3841 ggacaaaggg ttatacactg gcagatgttc gtgacacgct caaaaatgca gggtatgaag
     3901 taggagagaa ggcacttcgt gacattattc gggaagcaga atcgaaaaaa ccatcccgcc
     3961 gctcttcctc taaaacagca cctaaaaaga cagattcagg caggaaagat ggcatcgata
     4021 tgaacaacaa ttgatgtgac aatgtcacat tgtgttctga gattatgatg attacagaaa
     4081 aaaaagcccc ggcataaata ccagggcttt tctgtttaat cagactcgtg gcggcaatac
     4141 acctggataa tgccgtgcaa taatatcatt cagtttttca acaaggtcag aacgcttaaa
     4201 gacaatgtgt acggtccctt tctggtagcc acgcaccgta aaatattccc cttctaacag
     4261 cttaccgaca ttgtcgccgt tcccgagaaa ttcaccatac tgcgccccga tgctgttcct
     4321 gaagtcagga acgttgcgcc cctccagcag ataaaacgga cgtaccaggt catccagtgc
     4381 ctcccgtccg ctgcagtcca gcgtcacacg cccgttactc cagcggtaca gcaggttttc
     4441 aataatgatt ttcttaccca gacggcaggg attattggtt ctgtaatccc atgacaattt
     4501 ccggtagaca tcaatcagtc cctgttcaaa tgtctgcatc ttactggcat tcagatggcg
     4561 gaatgttgcc agtacattgt ccagggtgat ttcaggacag gtgtcgcaca tcagctggct
     4621 gttccactca tcacgctggc atgaactcat gaaggtgtac atgcccgtat ctgtcatcag
     4681 gcggtcccag attttccggt cggcaagacg ggtgatattc cttttcgctt cttccggctt
     4741 atccatgcga cgaacggaat cagtcacgca gtcttcaaac ccgtaaagaa aaatatcacc
     4801 tgacgcatcg agcagaatcc tgcgcgctgt cgccaggcat tccatcgcct gcatgaacag
     4861 tgcaatacct tcgttacgct gcgcgatgat gcggtcgata gccacagacg gaataacatc
     4921 tgtcgtgccg gcagtattca ggtcagcagt gggtaaagtc tgattcatca cattgtctcc
     4981 tcatcgtcac gactgccggg tcagccaggt catttcccgg aggagcgaag ctccttctgc
     5041 cgtccgggtc ttagggtatt tccccgcagt ggacctttcc gacgggggac gctcaccacg
     5101 tcacggggca gttgtccggg ggcagacgca aaagtttttg acgcctgcgg caaaaacttt
     5161 tgtggctggt acatctctcc cctggacgac tgaaacggga tgtggtcgtg tatgtacccc
     5221 cggaaagtcc gtctgcggag aacaggccgc agtgcggcgg cagacaggca gagacagctg
     5281 cgccatcagg cgcagtggcc ttacacaccg taaggtgtgc agggagcgcc gcatacggcg
     5341 cgactgaaga aaaacgacgg cacaacaatg gcatgtggtg gtcactgaca caccactccc
     5401 gcacaggtga aaacagagaa aaccgcacga catttacaag caaaaaaaag tgaaatttct
     5461 tcactttttt acacttattt atcgcgctga tttggcaatt gtgatgataa aataaagtga
     5521 agttttttca ctttttcaaa ggtgttttat gaccggctgg gaactgcgaa tctggcgaaa
     5581 aagcatgctc tggtcacgcg aaaaagcggc cagagaattt ggcgtgacgc agagaacatg
     5641 gcacgcctgg gaaaatgcgg aacaggtgga cgtcaccgtc tggaggacca cccaggcact
     5701 ttcagtccgt gacctgctgc ctcacatgca ggggatgcgt aaggcagata tcatcaggcg
     5761 actggaaaat gaactggggg aaacggcagg gaatgtgtga gcgtgattca cagcctggct
     5821 gaatgccagg ctactgctca tcaggaaaca gctttttcag ttcttcaccg gaaagtcccg
     5881 tgacctcctg aatccgttcc ggcggccagc ctatatcccg aagccggcag gctatttcca
     5941 gcgccacttc aagagcccct tgtttaaaac cctcgtcgaa acctttctgt ataagacgtt
     6001 ctgcaatcgt catgctgttt gctctgctcc tgctgacaaa aaagcccggc attttgccgg
     6061 gctttgtggt tctcatcagt gagtgatttt cttcagttcg tcatcggaaa gtccggtagc
     6121 ctgcttaatc tgctccggcg tcatccccat tttctgaagc tggcacgcaa tagccagggc
     6181 tttttcaagc gctccttcca tgcgtccttc catcctgcct tcctggcgac ctttttgctc
     6241 aagacgttct gcgatagtca tcagtttatc ctcatgctgc ggcagacgct cagccagcgc
     6301 ccgcataaat tccggtgata cagagtcccc tgcgttaacg atgtaatgaa tcagaacacg
     6361 cacctgctcg tctgaaattt ccaccatgac ctgcgggagt ttgtccagca gctccatcat
     6421 gtcacgatgg cggatatgct tcatcagcag cgtcagtgcg gccatccggc gatggttcat
     6481 gatttcattg tcatcaacga cagtgatatc aaccagcgga aacggctttg tgtatatttt
     6541 agccgcaagt gccggattct caaaacagtc cagccagttc atgctgtacg gatacgggct
     6601 gcgctcacca tgatagaaca gcaccggaat caccagtggc aacgttttgt gctgctccag
     6661 atgtctctgc atcgccgcca cactgtaacg taacatgcgg aaaggcatgt acagattttc
     6721 ggtgctctgg tgctcgatga gcgtataaac atagccatct tcacccccgg tggttttcac
     6781 gctccagagg atatcgctgg cgtactgatg caggtccggc tcaacaaagg tggcgggttc
     6841 cagcttcagc gtggacaggt cgcacaactg ccggtactcc gccggaagat gcagttccag
     6901 aaaatctctg gcgacgtcgg gattcgccag gaacgaccgg aacgcggcat catgcggcgt
     6961 gggcgttgtg gtgtttttct tcttactcac ggttcctcct ggtgagatgt ctgaacgtat
     7021 cctgccgcct gcacaggcag acggcaaggg ttaatcagtg gcagacccgc tcaatttcaa
     7081 gcgcgcggag caggtggccc cattcgctga ctttcatagc ggggatacgc acccgcttct
     7141 gctcactgca gcgtctggcg atatggccgc tcatcgccag gacgtccggg ttaatctcat
     7201 cctgtgccag ctcctgacgt ctgaagcggg tatggcggtg cagaggttta ttctcccgca
     7261 ggcactcttc acgcagacgc tttacatact cggcggttgc gaagataatt gatggtctca
     7321 tggcataatc tccatagata gtgattgaga tgccggactt cctggcgggg aagcgccggc
     7381 ggagagaggc agggtttatc gccctgcctt tttttcattt cggacagcga cgcagtgcca
     7441 gcgcgagcgc tttcatcttc cagccttccg gcgtgcggta catcaccttc cagtagccgt
     7501 cgatgaacac ataatggaag cgcacctctt cagtgctttc agtggcaata gcctccatga
     7561 aagaacggta ctcacgcgcc tcactgtcca tccggaaacc actgtcacgt ccgtaataag
     7621 ccgtcacacc gtccaccggg ttctgaagcg tatgcccggc gggtttgtcg caacggggac
     7681 cgaggcaacg gatatcaccg tgttcaacca gggcttcggc ctgctgaccg gtggcatagt
     7741 gggtatgcag gatgcgaccg ttccaggtcg gctcaccgtg caggtggcag taaacggtac
     7801 ggaacacccc gtcgcggcaa aaaacggaaa tcgtgctgct gacagtcatg aaaacgtctc
     7861 cttctgctga atcaggtcgc cgggccgacc ggttgcaccc cggaggggcg aaagcccctt
     7921 ctgccgtcca cttcttcggg gtttctcccc gcagtggacc tttccgatgg ggcgtccgtc
     7981 accacgtcac ggggcagctg tccgggggcg gacgcaaaat tttttgccgg gtttatggct
     8041 aaaaattttg tggacggtat atcttcccct ggactgctga aacgggatgt ggttactcca
     8101 gcccccggaa agtccacagc gggaaacagg ccgcaggggt ggcggcggaa gggttgactg
     8161 cgccatcagg cgcagtggcc ttacacaccg tcaggtgtgc agggagcgcc gtttccggcg
     8221 cgacaggggc ggcgcgaccg gaacggtcgc tgcaagcct
//
LOCUS       DAZPJP010000176         8240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPJP010000176 DAZPJP010000000
VERSION     DAZPJP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.177"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(408..1604)
                     /gene="hemY"
                     /locus_tag="ACHU9J_003474"
     CDS             complement(408..1604)
                     /gene="hemY"
                     /locus_tag="ACHU9J_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1906932.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1607..2824)
                     /gene="hemX"
                     /locus_tag="ACHU9J_003475"
     CDS             complement(1607..2824)
                     /gene="hemX"
                     /locus_tag="ACHU9J_003475"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1906933.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="ACHU9J_003476"
     CDS             complement(2846..3586)
                     /gene="hemD"
                     /locus_tag="ACHU9J_003476"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1906934.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="ACHU9J_003477"
     CDS             complement(3583..4524)
                     /gene="hemC"
                     /locus_tag="ACHU9J_003477"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1906935.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4911..7457
                     /gene="cyaA"
                     /locus_tag="ACHU9J_003478"
     CDS             4911..7457
                     /gene="cyaA"
                     /locus_tag="ACHU9J_003478"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1906936.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="ACHU9J_003479"
     CDS             complement(7497..7817)
                     /gene="cyaY"
                     /locus_tag="ACHU9J_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1906937.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7979..8121)
                     /locus_tag="ACHU9J_003480"
                     /pseudo
     CDS             complement(7979..8121)
                     /locus_tag="ACHU9J_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1839 a         2172 c         2141 g         2088 t
ORIGIN      
        1 ttattaattg aatgaatttt atcattcata ataagtatgt gtaggatcaa gctcaggtta
       61 aatattcact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      121 gttatttccc gattctctgt ggcataataa acgggtagat gctcattcca tctcttatgt
      181 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      241 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      301 cactgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      361 atgcatcaga aaggtcccgg aggtgcttgc ctccgggtga gaaggaacta ctgtggtggg
      421 ttgttctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      481 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      541 agcgctgcgc ggaaggcgag cgatgcttcc tgccattctc cgtgcttcat cagtgactgg
      601 cccagtgtgc tccacaacag cgggcgatcg ccgacgtttt tgatctgctg gcgcagcact
      661 ttttccagct gttccggatt gttggttttc agtcgaggaa tcggcagcag taggcgatcg
      721 tcgtactggc gtttcaggcc atcgatgata atttgctggg cagtatcatg atcgtcacat
      781 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
      841 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
      901 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
      961 ttcgccattg atgggatgat atccagcaga gaactccatg cacctgtgcg gatatacgcc
     1021 tgttccgcca gacgtaatac ttccggatgg cgtggcgtaa cttccagcag cttatccacg
     1081 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1141 accggaatgg tgtcattgcc ggccagctct gctgcgcgtt ccagatgttg gttggcgcgt
     1201 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1261 tccgcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc gccttccgcc
     1321 agtttcagca gcgcctgttc ggtctgctta cgggcacggc gacgcttacg tccgacaaac
     1381 cacccacggg tgtgtgcgcc tgtgcggaag atccgccgca gtagccactc aatggcaaac
     1441 agcactacca tcgccagaat caaaataatc gccaggcccg tgacgctggt ttcgatattg
     1501 tagttgtcag tctggatcag cacgtaaccc tgatgaccgg caatcatcgg gccaaccact
     1561 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1621 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     1681 caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac
     1741 gcacgcgggt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat
     1801 ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg
     1861 catcatcagt atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat
     1921 aagtctcttc ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat
     1981 tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac
     2041 ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc
     2101 attcgctgat ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt
     2161 cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga
     2221 tgccgtcata atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc
     2281 gacgaacggt aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca
     2341 gcaacgctgc agcggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca
     2401 ccagaaaatc ggcctgggcc agcagccagg ttttagcatc gctgccggaa atggtggcga
     2461 ccttttgctg gacttcatcc agctgttttg ccagcgtttc ttgctgacga ttcgcctgtt
     2521 caagctgcgt agcctgttgc ttaataatgc cttccagttc ggctttttgg ctctcctggg
     2581 ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg
     2641 cctgttgttt accccagcca tacaaaccga cgcccgccgc cagagcaata gcgatagccg
     2701 ccgcgctgag aatcaatgcg gtattgttct tactcttttt ttctgttgcg acaggttgtg
     2761 acgtggtgtc cacggcctcc ctagtctctt caaccacggc ggaggttttt tcttgttccg
     2821 tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttggcag
     2881 cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct
     2941 cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca
     3001 gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccgtcgtc acctcgcggg
     3061 attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt
     3121 cacaaaaagt gacctcagca ccgcgcgccg tcagggtatc cccaattagc tcacgaccgc
     3181 cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta
     3241 gcaagacttc gctgatttcc cgatcctgcg ggtagagaat cttctgtccg cttacggtat
     3301 gtagtgccag tgcggtggtg cgtccaatgg cgaaataatt aggtagtcgg ggccatttac
     3361 gatcttgctg atgtagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca
     3421 acagatcgct ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgttgacccg
     3481 gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca
     3541 ctaactcttc tccagcggga gacgggcggg tgacaaggat actcatgccg gggcgtctcc
     3601 gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga
     3661 aatccccatt tgtttggcat tttgcggcgc accgcggcgt tcaccgcgaa taatctgcga
     3721 accgtccggc gcgccgacca gcgcacgcag ccagatttcg ccatcaataa gctcggcgta
     3781 gctaccaatt ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt
     3841 aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgcgtatc
     3901 atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg
     3961 tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag
     4021 aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg
     4081 cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact
     4141 ggacgtcccg acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac
     4201 aaaggcatcg cgaggatctt cacgctcaca aatagtgacc agtcccagac cttgcgggaa
     4261 ttcaaccggc acatctttca ttgaatgtac ggcgatatcg gcgcgatttt cgaggagcgc
     4321 gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag
     4381 aatcacatcg ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc
     4441 catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc
     4501 aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta
     4561 acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta
     4621 aaatgaggtc tggcagtgga tcctgacaga cgtttcacgc tgtcgtaata aggaatttac
     4681 agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag
     4741 gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc
     4801 gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt
     4861 gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct
     4921 atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc
     4981 ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact
     5041 atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt
     5101 acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt
     5161 cagtacagga tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca
     5221 cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct
     5281 ggctcgatag cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg
     5341 ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc tttcgtcata
     5401 atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg
     5461 acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc
     5521 cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc
     5581 tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact
     5641 ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga
     5701 aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag
     5761 atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca
     5821 tgatgctgga gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt
     5881 tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg
     5941 taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc
     6001 gtctggcaat gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca
     6061 acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca
     6121 ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt aagctgtatg
     6181 ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc
     6241 tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt
     6301 ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat
     6361 ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggtctg ctgacctcgc
     6421 gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg
     6481 tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct
     6541 acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat gacccgacag
     6601 cggcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg
     6661 gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg
     6721 aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc
     6781 tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc
     6841 agcatctgcg cgggttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac
     6901 tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc
     6961 aaacctgggg cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca
     7021 tcgagtttta tggcgctatt tcgcataaca aactgcacgg cctgtcagtg caggttgaaa
     7081 ccaatcacgt caaattaccg gcggtggtgg acggctttgc tagtgaaggg atcatccagt
     7141 tcttcttcga agaaacgcaa gacgagaatg gctttaatat ctacattctt gacgaaagca
     7201 accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggaactggtg cgtgacgtca
     7261 gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca
     7321 acctgccgca gttctatcag attgtgaagg ttgatggtcg tgaacaggtg attccgttcc
     7381 gcacaaaatc tatcggtaac atgccgcctg ccaatcagga tcacgatacg ccgctattac
     7441 agcaatattt ttcgtgatga acgtgccgga aagcgaggct tatccggcat gcaatcttag
     7501 cggaaactga ctgtttcacc cgcctgctgc gtcgccgcct gttccagcaa atcccagaag
     7561 gtttcgccgc tgcgatcgca aatccactca tcgcctttca gatcaaaatg gtagccgcct
     7621 tgtttggttg ccagccatac ctggtgcagc ggttcctggc ggttgataat gattttgctg
     7681 ccgttttcaa aggtaatggt cagcacgccg ccgttgattt cgcagtcgat atcgctgtcg
     7741 ccatcccagt cgtccaggcg ttcttcaatg gtcagccaga gttgatcagc caggcgatga
     7801 aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt atagcggcat
     7861 ggggtcaggg cttcaaagtt tgcacctctg cggctgcgtt ccggcacgat tcatccgtca
     7921 ccggaataat gatgtctctg tgtagcgaaa gatttgtctc ttcattaggg cgcagttaca
     7981 ccacgccttt ccctgtttct ggtaaacatt atgatcaggt ttaccgagcg agcatcctca
     8041 cgctgacgga actaaaaaag acaacaaaca aaccacattg cgatagtgca tagagccatc
     8101 ctggcgcgag gtgccgatca cgaaactacc agcaaaacat aaatccccac gagtaagcgt
     8161 tatactcgca gcatttcctc acttttcaga cttcataaag agtcgctaaa cgcttgcttt
     8221 tacgtcttct cctgcgatga
//
LOCUS       DAZPJP010000177         8213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPJP010000177 DAZPJP010000000
VERSION     DAZPJP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.178"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..99
                     /gene="mgtT"
                     /locus_tag="ACHU9J_003481"
     CDS             <1..99
                     /gene="mgtT"
                     /locus_tag="ACHU9J_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1906938.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(214..1104)
                     /gene="dgcZ"
                     /locus_tag="ACHU9J_003482"
     CDS             complement(214..1104)
                     /gene="dgcZ"
                     /locus_tag="ACHU9J_003482"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR1906939.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1359..1751)
                     /gene="ydeI"
                     /locus_tag="ACHU9J_003483"
     CDS             complement(1359..1751)
                     /gene="ydeI"
                     /locus_tag="ACHU9J_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1906940.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2027..2545
                     /locus_tag="ACHU9J_003484"
     CDS             2027..2545
                     /locus_tag="ACHU9J_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1906941.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2590..4635)
                     /gene="dcp"
                     /locus_tag="ACHU9J_003485"
     CDS             complement(2590..4635)
                     /gene="dcp"
                     /locus_tag="ACHU9J_003485"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1906942.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4772..5518
                     /gene="ydfG"
                     /locus_tag="ACHU9J_003486"
     CDS             4772..5518
                     /gene="ydfG"
                     /locus_tag="ACHU9J_003486"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1906943.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5607..6293
                     /gene="rspR"
                     /locus_tag="ACHU9J_003487"
                     /gene_synonym="ydfH"
     CDS             5607..6293
                     /gene="rspR"
                     /locus_tag="ACHU9J_003487"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1906944.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6470..6673
                     /gene="ydfZ"
                     /locus_tag="ACHU9J_003488"
     CDS             6470..6673
                     /gene="ydfZ"
                     /locus_tag="ACHU9J_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1906945.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6709..8169)
                     /locus_tag="ACHU9J_003489"
     CDS             complement(6709..8169)
                     /locus_tag="ACHU9J_003489"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1906946.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2170 a         2054 c         1871 g         2118 t
ORIGIN      
        1 ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt
       61 tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg gatagatttg
      121 tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt tgacacagta
      181 gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca cattttgttc
      241 gtcaataaac atgcagcgat ttcttccggt ttgcttaccc tcatacattg cccggtctgc
      301 tcttccaatg accacatcca gaggctcttc aggaaatgcg cgactcacac ctgctgtcac
      361 ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta actggcaaat
      421 tctgacacct gcacgacatg cttcttcatc attagccgct ttaacaataa tgataaattc
      481 ttcgcccccg tagcgataaa ccgtttcgta atcacgcgtc caactggcta agtaagttgc
      541 cagggtgcgt aatactacat cgccgattaa atgcccgtag gtatcattaa ccaatttaaa
      601 tcggtcaata tccaacaaca ttaaataaag attcagagga tcagtgttgc gtaactgatg
      661 atcaaaggat tcatcaagaa cccgacgccc cggcaatccc gtcaaaacat ccatattgct
      721 acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag aaagcaaccc
      781 ctcctgaaag gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag ccagcattaa
      841 ttcccgacca cagttatgca tatgttgatg ggcagaatcc attagccgaa cgtaaggtaa
      901 ttcatcgtta tcgagtggcc ccagatgatc aatccaccga ccaaactggc acagtccata
      961 agaatggtta tccgttattt ctggcttact ggcatctctc gcgaccacgc tgtgaaacat
     1021 actcaccagc cactggtagt gggcatcgat agccttattg agatttaaca agatggcatc
     1081 aatttccgtt gtcttcttga tcattgccac tcctttttca cagttccttg tgcgcgctat
     1141 tctaacgaga gaaaagcaaa attacgtcaa tattttcata gaaatccgaa gttatgagtc
     1201 atctctgaga taacattgtg atttaaaaca aaatcagcgg ataaaaaagt gtttaattct
     1261 gtaaatcacc tctgcattat cgtaaataaa aggatgacaa atagcataac ccaataccct
     1321 aatggcccag tagttcaggc catcaggcta atttattttt atttctgcaa atgagtgacc
     1381 cgaacgacgg ccggcgcgct tttcttatcc agactgccgc taatgttgat catctggtcc
     1441 ggctgaactt ctcgtccatc aaagacggcc gcaggaataa caacattaat ttcaccgctc
     1501 ttatcgcgaa aaacgtaacg gtcctctcct ttgtgagaaa tcaaattacc gcgtagtgaa
     1561 accgaagcgc catcgtgcat gttttttgcg aaatcaacgg tcattttttt tgcatcatcg
     1621 gttccgcgat agccatcttc tattgcatga ggcggcggtg gcgctgcatc ctgttttaaa
     1681 ccgccctggt catctgccaa cgcataaggc atgacaagaa aacttgctaa tacaatggac
     1741 tgaaatttca tactaactcc ttaattgcgt ttggtttgac ttattaagtc tggttgctat
     1801 ttttataatt gccaaataag aatactgcca attgttataa ggcatttaaa atcagcccac
     1861 tagctgtcaa atattcagag aatttgactc actaaagtta agaagattga agagtcttaa
     1921 acatgttttc agaataatcg gatttatatg tttgaaaatt attatattgg acgagcatac
     1981 agaaaaagca aatcaccttt acatataata gcgtggacaa aaaacagtga acattaataa
     2041 agataaaatt gtacaactcg cagataccga cactattgaa aacctgacat ccgcgttgag
     2101 tcaaagactt atcgcggatc aattacgctt aactaccgcc gaatcatgca ccggcggtaa
     2161 gttggctagc gccctgtgtg cagctgaaga tacacccaaa ttttacggtg caggctttgt
     2221 tactttcacc gatcaggcaa agatgaaaat cctcagcgta agccagcagt ctcttgaacg
     2281 atattctgcg gtgagtgaga aagtggcagc agaaatggca acaggtgcca tagagcgtgc
     2341 ggatgctgat gtcagtattg ccattaccgg ctacggcgga ccggagggcg gtgaagatgg
     2401 tacgccagcg ggtaccgtct ggttcgcgtg gcatattaaa ggccagaact acactgcggt
     2461 tatgcatttt gctggcgact gcgaaacggt attagcttta gcggtgaggt ttgccctcgc
     2521 ccagctgctg caattactgc tataaccagg ctggcctggc gatatcccca ggccagccat
     2581 tggtggtgtt tatatgttca agccacgatg ttgcagcatc ggcataatct gaggagcctt
     2641 accgcgccat tgtcgatgca ggcgttccag atcttcgctg ttacctctgg aaaggatcgc
     2701 ctcgcgaaaa cgctgcccat tttcacgcgt taatccgccc tgctcaacaa accactggta
     2761 accatcatcg gccaacattt gcgtccacag ataagcgtaa taacctgcag catatccgcc
     2821 accaaaaata tgggcgaaat aactgctgcg atagcgtggc ggtatagcag gaagatccat
     2881 attttccgcc accagcgccc gcaattcaaa atcatcgaca tcctgcattg cttcgttttc
     2941 ttccaggcaa tgccagcgca tatcgagaag tgcggcgcta agcagttcgc tcatctcata
     3001 ccctttgttg aacaggctgg cattacgcat tttctgttgc agttcgtcag gcattgctgc
     3061 cccgctctga taatgccggg cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc
     3121 gttgatttgc gacggaaatt cgacaaaatc acgcggcgtg ttggtgccgg aaagcgtggc
     3181 ataacgctgg cgggcaaaaa ggccgtgcag cgtatgacca aattcatgga ataaggttat
     3241 tacatcatcc cagagtaaca acgcaggctc accggcagcg ggtttctgat aattgcagac
     3301 gttataaatt accggatgcg tttcattaag cgttgattgc tcaacaaaat tgcccatcca
     3361 tgcaccgccg ctttttgaat cacgggcgaa gaaatcaccg taaaataacg ccagccccac
     3421 gccattatga tcaaaaattt cccacacccg aacgtcagga tggtagacag gaatatcaaa
     3481 acgttcgaca aacttaatac cgaagagctg attcgcggtc cagaatacac cttcattcaa
     3541 caccgtgttt aattcaaaat atggcttgag ctgcgcttca tcaagatcaa atttctcccg
     3601 ccgtacctgt tccgcataaa atgcccagtc ccacggctgc gcgctaaacc cgccctgctg
     3661 cttatcgata accgcctgta tggaggctaa ttcatcgctc gcacgttgac gcgccgctgg
     3721 aacaatttcc cgcataaagt tgagtgctgc ttctggcgtt tttgccatct gatcggcgat
     3781 tttccatgcg gcataatgag gaaaaccaag cagtttcgcc tgctgtgcgc gaatctctac
     3841 cagacgttga atgatagcgc gggtatcatt gccatcattt ttttccgctc gcgtccagcc
     3901 cgcaataaac agtttttcac gcgtcgcacg atcgcgcatt tcggcaagcg ccggttgttg
     3961 ggtggtattc agcagcggaa tcagccattt gttatccaga cctttctcgc gagccgcctc
     4021 tgccgccagc gcaatctctt gctcactcat tcctgccagc tgcgcgatat cgttcacaac
     4081 cagaccgccg gatttatttg ctgccagtaa tcgctggtta aactggctgg tcagggtcgc
     4141 agcttctgta ttcagtactt ttaattttgc tttatcagct tgcgcaagtt tggctccggc
     4201 aaggacaaaa cgttgatgaa tcacctccac caggcggatg gattcactat caagccccag
     4261 ggattcacgg cgctgccaga cagcatctac ccgcgcgaat aattcaccgt tcagatagat
     4321 atcattagcc agttccgcca gttcagcgga aaactgttca tcaagacgct gtaattcatc
     4381 attggtatgc gccgcagtca tcgcaaaaaa gacgctggta acgcgggtaa gtaattctcc
     4441 gctttgttcc agtgccagaa tagtattgtt gaaatcaggc gtttgcgggt taagcgcgat
     4501 ggcagcaatt tctgcccgct tttgctgcat tccctcatcg aatgccgggc gatagtgatg
     4561 attggcaatt tgatcaaaat ggggagccag atacggtagt gtgctttgca caaggaaagg
     4621 attcattgtt gtcattttct tctcctgaac gcgagatgtt ccatagcgta ggcttactga
     4681 taacggagtg caatcttgca aaccagtatt acccgctctt aagcatcacg tgctatgtta
     4741 ttgacacaca aaagcgttga ggaacagtga gatgatcgtt ttagtaactg gagcaacggc
     4801 aggttttggt gaatgcatta ctcgtcgttt tattcaacaa gggcataaag ttatcgccac
     4861 tggccgtcgc caggagcggt tgcaggagtt aaaagacgaa ctgggagata atctgtatat
     4921 cgcccaactg gacgttcgca accgcgccgc tattgaagag atgctggcat cgcttcctgc
     4981 cgagtggtgc aatattgata tcctggtaaa taatgccggc ctggcgttgg gcatggagcc
     5041 tgcgcataaa gccagcgttg aagactggga aacgatgatt gataccaaca acaaaggcct
     5101 ggtatatatg acgcgcgccg tcttaccggg tatggttgaa cgtaatcatg gtcatattat
     5161 taacattggc tcaacggcag gtagctggcc gtatgccggt ggtaacgttt acggtgcgac
     5221 gaaagcgttt gttcgtcagt ttagcctgaa tctgcgtacg gacctgcatg gtacggcggt
     5281 gcgcgtcacc gacatcgaac cgggtctggt gggtggcacc gagttttcca atgtccgctt
     5341 taaaggcgat gacggtaaag cggaaaaaac ctatcaaaat accgttgcat tgacgccaga
     5401 agatgtcagc gaagccgtct ggtgggtgtc aacgctgcct gctcacgtca atatcaatac
     5461 cctggaaatg atgccggtta cccaaagcta tgccggactg aatgtccacc gtcagtaatt
     5521 tttatacccg gcgtaactgc cgggttattg cttgtcacaa aaaagtggta gactcatgca
     5581 gttaactcac tcacaagcaa gaacgaatga ccgtcgaaac gcaacttaat cccacacagc
     5641 ctgtcaatca gcaaatttat cgtattcttc gtcgcgacat tgtccattgc ctgattgcac
     5701 caggcacacc gttgtcggaa aaagaagttt ctgttcgttt caatgtgtca cgccagccgg
     5761 ttcgtgaagc ctttattaaa ctggcggaaa acggcctgat tcaaattcgt ccgcagcgtg
     5821 gcagctacgt caacaaaatt tccatggccc aggtgcgcaa tggcagtttt atccgtcagg
     5881 ccattgagtg cgcggtggcg cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc
     5941 aactggaaca aaatcttcac cagcaacgca ttgccattga gcgcaagcaa ctggatgatt
     6001 tttttgaact tgatgacaac ttccatcaac tcctgacgca gattgccgac tgtcaactgg
     6061 cgtgggatac cattgagaac ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg
     6121 accacgtttc tccaccagaa atgctgttac gccagcatct tgatattttc tctgccctgc
     6181 aaaaacgtga tggcgatgcg gtagaacgtg caatgacgca acatttgcag gaaatcagcg
     6241 aatccgtgcg ccagatccgc caggaaaata gcgactggtt tagcgaagag taattcattt
     6301 cctctcatcc catccggggt gagagtcttt tcccccgcct tatggctcat gcatgtatca
     6361 aaaaagatgt gagcttgatc aaaaacaaaa aatatttcac tcgacaggag tatttatatt
     6421 gcgcccgtta cgtgggcttc gactgtaaat cagaaaggag aaaataccta tgacgaccta
     6481 cgatcgtaac cgtaacgcaa tcaccactgg cagccgtgtt atggttagcg gcaccggtca
     6541 cactggcaag atcctgtcga ttgatactga aggtctgacc gctgagcaaa tccgccgcgg
     6601 aaaaaccgta gttgttgaag gttgtgaaga gaaactggca cctctggacc tgattcgtct
     6661 cggcatgaac taagcgtgtg aatgccgccg atggcggcat tgcttttttt acttcacgga
     6721 atatttcgcc acggtcgctt tcgcgccatg cgctaataaa gacaagtacg cttccgtcac
     6781 ttttgcagta aacaaactat tgtctggcaa atcatcacca aagatcgcct taatcgccag
     6841 caatgactgg acgcgcgctt tcccttcagc actactttgt acagccttct gaataacagg
     6901 taacagtggg tcactgattt caatcggatt tccctgttca tcaacaccac cgacataacg
     6961 catccaaccc gcgacgccca gtgccagcag atcgaacttg ctgtcatgcg ccagatgcca
     7021 gcgaacagaa tccaacatcc gctgtggcaa tttctggctg ccatccatcg caatctgcca
     7081 ggttcgatga cgtaacgccg ggttgctata gcgttcaatt aatcggttag cgtaatcttg
     7141 caaatcaacg ccctgcactt tcagcgtcgg cgcttgttcc tgcaacatca agccatacgc
     7201 cgcataacga taatgttcat cttccatgca gtcattaatg tgctgatatc cagcaagata
     7261 acccaaatac gccaggaatg aatgactgcc gttgagcatg cgcaacttca tctcttcata
     7321 aggcagcaca tcactaacca gttcggctcc cgctttttcc cattctggac gtccggcaac
     7381 aaagttatct tctattaccc actggcggaa aggttcacag gcaacgcccg caggatcgcc
     7441 cacaccggta agttgttcga ttttcgccag cgtatcctct gtcactgcgg gcacaatacg
     7501 gtccaccatt gttgatggga aagtcacgtt atcttcgatc cattgtgcca gttttacatc
     7561 aacagctttc gcgtatgaag tgacaacgtc acgcataaca tgaccgtttt ctggcatgtt
     7621 gtcacatgac atgacggtaa atgcgggaag tcctgccgct ttacggcggg ccagcgcctc
     7681 aacaatcacc cctgttgctg ttttcggctg gtggggattt tgcacgtcgg cagcgaccat
     7741 cgggtgatcg agcattaact gtccggtcgc cggagagtgg aaataccctt tttcggtgat
     7801 tgtcagagag acaatcgcga tttgcggttc acacatcgca gccaacacgg tttctaagcc
     7861 atccatctgt acgtgcaagg ctttcttaac gacgccaacg acgcgagccg tccacgcatc
     7921 ggccgacatt tccgcaacgg tataaagatt atcttgctgt tgtaaatcgg caatttgctg
     7981 ttcgccgccg attaagttga cctcataata tccccagtca ctgaaatgtt ctgtagcaag
     8041 aatatcggca tacacgccct gatgcgcacg gtgaaatgca ccaaagccta aatgaataat
     8101 tcttggggcc aggttattac gatcataaac agggagtgtc gcttttgctg ataacaaatt
     8161 atttcccata acaattcctt aaatataaat atggcaagct atatgttttg tta
//
LOCUS       DAZPJP010000178         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPJP010000178 DAZPJP010000000
VERSION     DAZPJP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.179"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHU9J_003490"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHU9J_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR1906947.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHU9J_003491"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHU9J_003491"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1906948.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHU9J_003492"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHU9J_003492"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1906949.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHU9J_003493"
     CDS             6679..>8182
                     /locus_tag="ACHU9J_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR1906950.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPJP010000179         8156 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPJP010000179 DAZPJP010000000
VERSION     DAZPJP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.180"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHU9J_003494"
     CDS             <1..254
                     /locus_tag="ACHU9J_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1906951.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHU9J_003495"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHU9J_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1906952.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHU9J_003496"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHU9J_003496"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1906953.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHU9J_003497"
     CDS             complement(2905..3180)
                     /locus_tag="ACHU9J_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1906954.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHU9J_003498"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHU9J_003498"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1906955.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHU9J_003499"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHU9J_003499"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1906956.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHU9J_003500"
     CDS             5140..5466
                     /locus_tag="ACHU9J_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1906957.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHU9J_003501"
     CDS             5650..6369
                     /locus_tag="ACHU9J_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1906958.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHU9J_003502"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHU9J_003502"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1906959.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8156
                     /locus_tag="ACHU9J_003503"
     CDS             7708..>8156
                     /locus_tag="ACHU9J_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1906960.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
BASE COUNT         2087 a         1987 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca tagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caatta
//
LOCUS       DAZPJP010000180         8142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPJP010000180 DAZPJP010000000
VERSION     DAZPJP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.181"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHU9J_003504"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHU9J_003504"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1906961.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="ACHU9J_003505"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="ACHU9J_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1906962.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHU9J_003506"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHU9J_003506"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1906963.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRRQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="ACHU9J_003507"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="ACHU9J_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1906964.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="ACHU9J_003508"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="ACHU9J_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1906965.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHU9J_003509"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHU9J_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1906966.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHU9J_003510"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHU9J_003510"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1906967.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2040 a         2086 c         2083 g         1933 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct tctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccccaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgttctcg
     3061 cgaacagcag ctggccatac accacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgccga cgatcttcat caccggtcag cgccaactga
     3241 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtacagggt tccacaccag aatatcgccg ttcagacccg catgacccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc gctcagcttg
     3421 ccgccaatcc cgacaaggaa taccgcgcca agatctttcg ctatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
     3541 tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacaa
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgctga agtcactgaa gcctattaca caacaggcca ctacagcatc
     4501 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccataatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggtagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
     6001 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
     6061 gcggtggtcg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
     6181 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccatcgatcg aagacaaagt catgcgcttc gccgacagaa
     6421 atcagcatca gatcttcctt gaaccggaag ggctgacctc taacgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatccac
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggtc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgagccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagctct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatcaaaaaa at
//
LOCUS       DAZPJP010000181         8074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPJP010000181 DAZPJP010000000
VERSION     DAZPJP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.182"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHU9J_003511"
     CDS             <1..188
                     /locus_tag="ACHU9J_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1906968.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHU9J_003512"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHU9J_003512"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1906969.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHU9J_003513"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHU9J_003513"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1906970.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHU9J_003514"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHU9J_003514"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1906971.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHU9J_003515"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHU9J_003515"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1906972.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHU9J_003516"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHU9J_003516"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1906973.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHU9J_003517"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHU9J_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1906974.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHU9J_003518"
     CDS             7142..7351
                     /locus_tag="ACHU9J_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1906975.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHU9J_003519"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHU9J_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1906976.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1817 a         1978 c         2212 g         2067 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc attt
//
LOCUS       DAZPJP010000182         7991 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPJP010000182 DAZPJP010000000
VERSION     DAZPJP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.183"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHU9J_003520"
     CDS             complement(152..487)
                     /locus_tag="ACHU9J_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1906977.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHU9J_003521"
     CDS             complement(491..835)
                     /locus_tag="ACHU9J_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1906978.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHU9J_003522"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHU9J_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1906979.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHU9J_003523"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHU9J_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1906980.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHU9J_003524"
     CDS             1257..1376
                     /locus_tag="ACHU9J_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1906981.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHU9J_003525"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHU9J_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1906982.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHU9J_003526"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHU9J_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1906983.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHU9J_003527"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHU9J_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1906984.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHU9J_003528"
     CDS             4176..4544
                     /locus_tag="ACHU9J_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1906985.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHU9J_003529"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHU9J_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1906986.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHU9J_003530"
     CDS             5295..5588
                     /locus_tag="ACHU9J_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1906987.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHU9J_003531"
     CDS             5660..6319
                     /locus_tag="ACHU9J_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1906988.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHU9J_003532"
     CDS             6411..6857
                     /locus_tag="ACHU9J_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1906989.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHU9J_003533"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHU9J_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1906990.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         2054 a         1796 c         1838 g         2303 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt t
//
LOCUS       DAZPJP010000183         7951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPJP010000183 DAZPJP010000000
VERSION     DAZPJP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.184"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHU9J_003534"
     CDS             <1..365
                     /locus_tag="ACHU9J_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR1906991.1"
                     /translation="ITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERL
                     GERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFL
                     LRKDIMRRLTQDVCEEIE"
     gene            complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHU9J_003535"
     CDS             complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHU9J_003535"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1906992.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHU9J_003536"
     CDS             3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHU9J_003536"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1906993.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4123..5169
                     /gene="aroH"
                     /locus_tag="ACHU9J_003537"
     CDS             4123..5169
                     /gene="aroH"
                     /locus_tag="ACHU9J_003537"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1906994.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5301..5492
                     /gene="hemP"
                     /locus_tag="ACHU9J_003538"
     CDS             5301..5492
                     /gene="hemP"
                     /locus_tag="ACHU9J_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1906995.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHU9J_003539"
     CDS             complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHU9J_003539"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1906996.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHU9J_003540"
     CDS             complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHU9J_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1906997.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2034 a         2086 c         1888 g         1943 t
ORIGIN      
        1 aaattaccgg acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg
       61 aagtagaaga tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc
      121 ccgatgaacg tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt
      181 cattatcgct ggaagaggtg gtggcttttt ttagccgtaa acgggtcgca aaatataaat
      241 atcctgaaca tatcgtggta atcgaaaaac taccgcgtac tgcctcaggt aaaatacaaa
      301 agtttttgtt aagaaaagat attatgcggc ggttaactca ggatgtctgt gaagagattg
      361 aataagtttc atcttcgggg atcacataac cccggcgact gaacgccgcc ggggatttat
      421 tttatttctt cagttcagcc aagcttaacc aggtttgcac cacggtgtcc gggttcagag
      481 acaggctatc gatcccctct tccatcaacc atgcggcaaa gtcttcgtgg tcggacggac
      541 cctgaccgca aatcccgaca tatttgccct gtttcttcgc ggcacggata gccatcgaca
      601 gcagtgcttt caccgcatcg ttgcgctcat cgaacaattc agacaccacg ccggagtcac
      661 ggtccagacc gagcgccagc tgcgtcatat cgtttgagcc aattgagaag ccgtcgaaat
      721 attcgaggaa ctgctcggcc agcaaggcgt tggacgggat ttcacacatc atgatgattt
      781 tcagcccgtt ctcaccacgc ttcagcccct gacgcgccag ttcctcaacc accgctttcg
      841 cctgatctac ggtacgcacg aacgggatca tgatctcaac gttggtcagc cccatgtcgt
      901 tgcgcacacg tttcactgct tcgcactcca gcgcgaagca gtcgcggaag ctgtcggaaa
      961 catagcgtcc cgcgccacgg aagccgagca tcgggttctc ttcatctggc tcgtaacgct
     1021 caccaccgac caggttggca tattcgttcg atttaaaatc agagagacgg acaatgacgc
     1081 gcttcggata aaacgcggca cccagcgtcg cgatcccttc agtcagacga ccaacgtaaa
     1141 attcacgcgg agaatcaaaa cctttcatca tctcgcggat ttcattttgc aactgcggtt
     1201 cctgatcgtc aaactcaagc agtgcgcgtg ggtggacgcc aatcatacgg ttgatgataa
     1261 attccagacg cgcaaggccc acgccttcgt tcggcaggca ggcgaagtcg aaagcacggt
     1321 ccgggttacc gacgttcatc atcactttca acggcagatc cggcatcatt tctacgctgg
     1381 agcttttcac gctaaattcc agcaactccg catagacgta accggtatca ccttcggcac
     1441 aagaaacagt gacgttctca ccgtctttca tccgttctgt tgcatcacca cagcccacta
     1501 ccgccggaat gcccagttca cgagcgatga tcgccgcgtg acaggtacga ccgccacggt
     1561 tggtgacgat ggcagatgct ttcttcatga tcggttccca gtccgggtcg gtcatgtcag
     1621 tgaccagcac gtcaccaggt tcgatgcggt tcatttcgct gatctcatgg atgactttca
     1681 ccggacccgc gccgatgcga tgaccgatag cacggccttc ggcgataatc ttaccctgtg
     1741 aatgcagcgt ataacgctcc atgacctgac cgcgtgagcg cacggtttcc ggacgcgcct
     1801 gcacaatgaa cagtttgcca gtgtggccat ctttcgccca ctcaatatcc atcgggcgac
     1861 cgtagtgttt ctcaatttgt acggcctgtt ttgccagttc ctgcacttct tcgttggtca
     1921 gcgagaagat gtcacgctgt tcctgcggta cgtcttcgat tttaacctgc ttgccgtgct
     1981 cctgggtcgg cgcgtaaacc atgcggattt ttttcgaccc catggtgcga cgcacgatag
     2041 ccgggcgatt cgccgccagt gtcggtttat ggacgtaaaa ctcatccggg ttaaccgcac
     2101 cctgcacgac catttcccca aggccccatg cggaagtgat aaacaccacc tggtcaaagc
     2161 cggattcggt atcaatggag aacatcacgc cagaagatgc gaggtcggag cgcaccatcc
     2221 gctgaacacc ggcagagagc gccacgccac ggtgatcgta accctggtgc acacgataag
     2281 agatggcgcg atcgttaaac agagaagcaa atacatgctt cactgccacg agaacggcgt
     2341 caaaaccctg aacgttgagg aaggtttcct gctgaccggc aaaagaagcg tccggcatat
     2401 cttctgcggt ggcggaggag cgcaccgcaa aagaggcgtt ttcgtcatcg gcggaaagct
     2461 gtgcataggc ttcgcggatg gcgttttcca gctcaggctg gaagggagtg tcgataatcc
     2521 actggcggat ttgcgcgccc gctttcgcaa gctgagtaac atcgtcaata tccgttttat
     2581 ccagcagttc ataaatgcgc tggtttacgc cgctttggtc cagaaactgg ttaaacgcgt
     2641 cggcggttgt ggcgaaacca ttcggaacgg aaacacccat tccggaaagg ttagtaatca
     2701 tttcacccag ggaggcattt ttgcccccaa ccctgtctac atcattcatg ccgagttggt
     2761 tataccaaag caccagcggt gacgagccat tgttggacat cgaacaatcc ttttgtgata
     2821 aatgaacggt ttgagaaaca catttctgcg catttatctt tgcatattta accggatgaa
     2881 aaaaacggtg aatcgttcaa gcaaatatat ttttttactt tttaagactg atcccagcgt
     2941 tgcgcaaatc tgctctcttc gacaatttcc acataaacca tcggtataaa cgaaaccata
     3001 aaaatgaaat gctgttttca taaaaaataa aattgaaggt tcattttata aaccagacat
     3061 aacgtcctac gcttctgtgc gtttttaatt tatgctttca tagaattatg tctgcatcac
     3121 gggaagaaca aaatggataa tgctgttgat cgccacgttt tttatatttc tgatggtacg
     3181 gcaataactg cggaggtatt aggacacgca gtaatgtcac aatttcccgt cactatcagc
     3241 agcattacgc tgccgtttgt cgaaaatgag agccgtgcac gggcagtgaa ggatcagatt
     3301 gacgcaattt atcaccagac aggtgtgcgc ccgctggtct tctactccat cgtgttgccg
     3361 gagattcgcg ccatcatctt gcaaagtgaa ggcttttgcc aggatatcgt tcaggcgctg
     3421 gttgccccgc tacaacaaga gatgaaactg gatccaacgc cgattgctca tcgtacccat
     3481 ggccttaacc ctaataatct caataaatat gatgcgcgca ttgcggcgat tgattacacc
     3541 ctcgcccacg atgacggcat ttcgttgcgc aatctggatc aggctcaggt gatcctgctc
     3601 ggtgtttctc gctgtggtaa aacccccacc agtctttatc tggcaatgca atttggtatc
     3661 cgcgcggcaa actacccctt tattgccgac gatatggata atctggtgct acccgcgtcg
     3721 ctcaaaccgc ttcagcataa attgttcggc ctgactatcg acccggaacg tctggcggcg
     3781 attcgcgagg aacgtcggga gaacagtcgc tatgcctcgc ttcgtcagtg caggatggaa
     3841 gtcgcggaag tggaagcgtt gtaccgtaaa aatcagatcc cgtggattaa cagtaccaat
     3901 tattcggtag aagagattgc caccaagatc ctcgatatca tgggccttag tcgccgaatg
     3961 tactagagaa ctagtgcatt agcttatttt tttgttatga tgctaaccac ccggcgaggt
     4021 gtgacacacc tcgcacttga aatcagcagc gattggttta tcgtgatgcg catcacttcc
     4081 cggcagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     4141 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     4201 gtaacgcctg gcgccgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactc
     4261 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     4321 gctgcaatgg agtacgcccc ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     4381 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaaggacta
     4441 atctccgatc cagatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     4501 aaattacttt tacaggtaaa tgagctgggc gtcccaaccg cgactgagtt cctcgatatg
     4561 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc acgtactacc
     4621 gaaagtcaga tccaccgtga aatggcttcg gcactctcct gtccggtagg ttttaaaaat
     4681 ggtaccgatg gcaatacgcg gattgctgtg gatgctatcc gcgcagcccg cgccagccat
     4741 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     4801 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     4861 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     4921 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     4981 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgctcgaa
     5041 ggaacgcaaa aaatcgtcgg cagtcagccg ctcacttacg gtcaatccat taccgacccg
     5101 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     5161 cgcttctgaa tgcgtgccca ttcctgacgg aatgggcatt tctgcgcaac ttgttgtctt
     5221 ctcaacaaat tactgcttgc tctggcctgc cataatattg ataataagaa tcattgttat
     5281 atcaattatt attaatcttt atgcgttata cggatagcag aaaactcacg cctgaaacgg
     5341 acgccaatca caaggccgct tccccgcagc ccatccggcg aattaccagc cagacactgt
     5401 taggtccgga tggcaaactg attatcgatc atgacgggca agaatatctg ctccgtaaaa
     5461 ctcaggctgg caagctgctg ttaactaagt agcctttaac tcgagcagct gacttccagc
     5521 cgtttacccc agtcaggtgg acgactgaca tagtcatcat ctctatcgct gaaaggattt
     5581 cgcaacgccc catgcaggcg gtgcaattcc gtcatatcac ccttttctgc cgcttcaatc
     5641 gcccgttgcg ccaaccagtt gcgcaatacc agagcggggt taacgctttg catcaactgc
     5701 tgacgctcac tatcgctaac ctcgtcttgt tgcaaacgcc cccgataacg ggcaaaccag
     5761 ccatcaaatg ccgcacgatc aataaactca tcacgtagcg gtgacgccgt gctgtgctgc
     5821 tcggtcagac tcagcatgcg gaatgtgcgg gtataatcgc tgcgctctcg cgccatcaga
     5881 ctgaataatt cattcagtag cgcgttatcc tctttttgct ccgtgatgaa gcccagtttc
     5941 tgccgcatcc gttgtccata atgcgtcaac aaaacctgct gatagctgtc cagggcctca
     6001 ttcagggcat ctacggcaac aaatggtgac aatgtctgcg ccagacgctg taaattccac
     6061 aacgcgacag caggttgatt atcaaaacta taacgccctt gatgatccga gtgattacaa
     6121 ataaaaccgg gttcgtaatc atcaagaaaa ccaaacggcc cgtaatcaag cgtcagtccc
     6181 agcagcgaca tgttgtcggt attcatcacc ccatgtgcaa agccgaccgt ctgccattgg
     6241 gcaattaacg acgcggtacg tgcgacaaca tcgctaaacc agagacggta tttgtcctca
     6301 tcatcttcaa gatgtgacca gtaatgacga atagcaaagt cagctaactg acgaaccttt
     6361 tccgactcgc ggcggtagta aaaatgttcg aaatgaccaa agcgcagatg acttggtgcc
     6421 acacgcatca gcatcgcgcc tggctccgcc gtttcccgat acactggcga atcgctggtg
     6481 acgatactta acgcgcgggt cgtcggaatg cccagataat gcatcgcctc actggcgaga
     6541 ctttctcgta tcgtcgaacg taaaaccgcc cgtccatcac ccattcgcga ataaggcgtc
     6601 aggccagcac ctttcagatg ccagtccatt gtagtgccat cagcaagcag ttgttcgccg
     6661 agtaaaatgc cgcgcccatc acccagttgg cccgcccaga cgccgaactg atgaccactg
     6721 taaacctgcg ccagtggtga cataccaggc agtagggctt cgccgcccca gacacctgcg
     6781 ccatttttaa acagcgacga tggaatactc agcgtgttag ccagttcggt attatgccaa
     6841 atcagccggg cattatttaa aggcgtaggg gaaagtgctg tataggtttc tggcaattca
     6901 tcgcgccagc gggtaacaaa agacagggtc atagatcctc ctgtttgata gtgtagacgg
     6961 ttactctcgt cttaaacacc agcaaacaga agggttatcg ctgaaccaac gtcgttatct
     7021 gatttatggg aacggcaggc cacaggcatc cctggagagc atggaaatcg aagggggtaa
     7081 tctgtgccaa tatttctgcc gtatcaatgc ctcccgcaat gatgcaattg caacaagggg
     7141 aaatttgcgc ctggatcgca cgaataaacg gttcgaagga gcggtgcgtg atttgttgct
     7201 gaataaaact tttatccagc atgacgcggg tgaaaagacc atcaaaaacc gctttcattg
     7261 tgctattgcc tgctcccaaa ttacctaaca caaggggata cacttgcgat aaggataaaa
     7321 gaccgcgatt atcttttcct tcattgagat gcgggtaatt ctcattaatc aacagttcaa
     7381 taaacggata ttttaatagt tcgcctgcgt aattatccct ctctaataat aatgtagcca
     7441 cttgtggcgt taaattaagc caggcaaaga gtttatgttg aataaaaaaa tgttggcatg
     7501 attttagtaa ttccaactgc tctgaaaata attgccaatg ttgctcctcg gtaagttgcg
     7561 ctataacccg actcgtcggg atacgcaccg tgccatcttc actggagaaa tgggtaatta
     7621 actcaacgcc gacaaggact tgctggttat ctcttatcgg caggaaatag caatcagaat
     7681 gataaagatt ctccaaaaaa atcttcatgg taaccgtccc tctcgaagga tgtttttcag
     7741 tatccggcta aaagggatga ggccataaga cataacaatt acagaaggag taactttcat
     7801 ttgttccatg ttaaccactt tttcaggggt ccttttttta gattatcctg attataaacg
     7861 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     7921 ggcgatgtat catcgccgtc catttttcga c
//
LOCUS       DAZPJP010000184         7943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPJP010000184 DAZPJP010000000
VERSION     DAZPJP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.185"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            124..735
                     /gene="ruvA"
                     /locus_tag="ACHU9J_003541"
     CDS             124..735
                     /gene="ruvA"
                     /locus_tag="ACHU9J_003541"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1906998.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            744..1754
                     /gene="ruvB"
                     /locus_tag="ACHU9J_003542"
     CDS             744..1754
                     /gene="ruvB"
                     /locus_tag="ACHU9J_003542"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1906999.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1901..2686)
                     /gene="znuB"
                     /locus_tag="ACHU9J_003543"
     CDS             complement(1901..2686)
                     /gene="znuB"
                     /locus_tag="ACHU9J_003543"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1907000.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2683..3438)
                     /gene="znuC"
                     /locus_tag="ACHU9J_003544"
     CDS             complement(2683..3438)
                     /gene="znuC"
                     /locus_tag="ACHU9J_003544"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1907001.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3517..4449
                     /gene="znuA"
                     /locus_tag="ACHU9J_003545"
     CDS             3517..4449
                     /gene="znuA"
                     /locus_tag="ACHU9J_003545"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1907002.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4465..5787
                     /gene="mepM"
                     /locus_tag="ACHU9J_003546"
     CDS             4465..5787
                     /gene="mepM"
                     /locus_tag="ACHU9J_003546"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1907003.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5907..6878
                     /gene="lpxM"
                     /locus_tag="ACHU9J_003547"
                     /gene_synonym="msbB"
     CDS             5907..6878
                     /gene="lpxM"
                     /locus_tag="ACHU9J_003547"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1907004.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6966..7943)
                     /locus_tag="ACHU9J_003548"
                     /pseudo
     CDS             complement(6966..7943)
                     /locus_tag="ACHU9J_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2110 a         1909 c         2011 g         1913 t
ORIGIN      
        1 atactgtgcc atttttcagt tcatcgagac acctcgcaag ttttcttcat ccttcgctgg
       61 atatctatcc agcatttttt tatcatacag cattatcttt gattcattac gcaggagcgt
      121 catgtgatag gcagactcag aggcatcatc attgaaaaac aacccccgct ggtgttaatt
      181 gaagtgggcg gcgtaggcta tgaagtgcat atgccgatga cctgttttta tgaactccct
      241 gaagcgggtc aggaagcgat cgttttcacc cactttgtgg tgcgtgaaga cgcgcaactg
      301 ctgtacggtt ttaacaataa acaagagcgc acgttgttta aagagttaat caaaaccaac
      361 ggcgtcggcc cgaagttggc gctggcgatc ctctccggaa tgtcagcgca gcagttcgtt
      421 aatgccgttg aacgtgaaga agtgggagca ctggtgaaac tgccgggcat tggtaaaaaa
      481 accgccgagc gtttaattgt cgaaatgaaa gaccgattta aaggtttgca cggcgatctc
      541 tttacgccag ccgccgatct ggtgctcacg tcgcctgcca gcccggcaac cgacgatgct
      601 gaacaggaag cggttgccgc gctggtggcg ctgggctata aaccacaaga agcaagccgc
      661 atggtgagca aaatcgctcg cactgacgcc agcagtgaaa cattaattcg cgaagcccta
      721 cgcgccgcgt tatgaggtaa aggatgattg aagcagaccg tctgatttct gccggtacca
      781 ctttgccgga agatggggca gatcgcgcca ttcgccctaa attactggaa gagtatgttg
      841 gtcagccgca ggttcgttca cagatggaga ttttcatcaa agcagcgaaa ctgcgcggcg
      901 atgccctcga tcatctgttg atttttggtc ctccggggtt gggtaaaact acgcttgcca
      961 acattgtcgc caatgaaatg ggcgttaatt tacgcacgac ttctggtccg gtgctggaaa
     1021 aggcgggcga tctggcagcg atgctcacta accttgaacc gcatgacgtg ctgtttattg
     1081 atgagatcca ccgtctatcg ccagttgttg aagaagtgct gtacccggca atggaagact
     1141 accaactgga tatcatgatt ggtgaaggtc cggcggcacg ctccattaaa attgatttgc
     1201 cgccgtttac cctgattggt gcaaccacgc gcgcaggttc gctgacatca ccgttgcgcg
     1261 accgttttgg tattgtgcaa cgtctggagt tttatcaggt gccggatctg caatatatcg
     1321 tcagtcgcag cgcacgcttt atggggcttg agatgagtga tgacggcgcg ctggaagttg
     1381 ctcgtcgcgc tcgcggtacg ccacgtattg ccaaccgtct gctgcgtcga gtgcgtgatt
     1441 tcgccgaagt gaagcacgat ggcaccatct cggcagatat cgctgctcag gcgctggata
     1501 tgttgaatgt cgatgctgaa ggtttcgatt atatggaccg caaattgttg ctggcggtaa
     1561 tcgataagtt ctttggtggg ccggtaggtc tggataacct ggcggcagcc attggcgaag
     1621 aacgtgaaac cattgaggat gtgctggagc cttatctgat tcagcaaggc tttttgcagc
     1681 gtacaccgcg tgggcgtatg gcgacgacgc gggcgtggaa tcactttggc ataacgccgc
     1741 cagaaatgcc gtaagtcgga ttggtcgctc gcaccgcatc cgacattggc agtgtgcctg
     1801 atgcgacgct attcgcgtct tatccggcct accagagata gcgaaccgta ggtcggataa
     1861 ggcgctcgcg ccgcatccga caaatgtgtt cagcgacaga ttagctggcc tgctttttca
     1921 tcatactgat aataaataac agtgcagcac atagcactac cgacggaccc gccggcgtat
     1981 cgtaaaacgc ggaaaaggtt aaaccgccag ttactgccac catccccacc aaaacagcga
     2041 caccagccat ctgttccggc gtacgggcaa agcgacgcgc agtagctgca ggaataatca
     2101 gcaacgaagt aataatcaac gcaccgacaa atttcatcgc tacaccaatc gtcaatgccg
     2161 tcaccagcat caacaacaat ttcacgcgct gtaatttcac accatcaaca aacgccagat
     2221 ccgggctaat cgtcatcgac agcaaattgc gccattgcca gaacaaaata gccaccacga
     2281 tgaccacgcc aatcgcaata gagatgagat cttctggcgt cactgccagc aaatcaccga
     2341 acaggtaagc catcaaatca acacgaatat tagacatcag actaacgacc accaggccca
     2401 gcgacagggc actgtgcgcc ataatcccta ataacgtgtc gatcgccagc tgtggacgct
     2461 tctccagcca taccagaccg cccgccagca gcagcgtaac ggcaatcacc gcatagaatg
     2521 gattcacgtc cagcaacaaa ccaaacgcga caccaagtaa tgaggcatga gccagcgtat
     2581 caccgaaata agacatacga cgccagacta caaacgaacc cagcggaccc gcggcacagg
     2641 cgagcatgat cccggctaac caaccgggaa ataataattc aatcatgagc gatcatttcc
     2701 ccgacgcaaa acaattcgtc cctgtaaatc gtgacgatgg ttatgatgat ggcgatagat
     2761 acccagttgt tcagcaccac gaggaccaaa cattgaaata aactccggat gcagggaaac
     2821 aacttccggt gtgccggaac aacaaatgtg gtgattcagg cacagcactt catcggtttt
     2881 tgccatcacc agatgcagat cgtgagaaac cattaaaacg ccacaatcca gttcacgacg
     2941 cagttggtca ataaggtcat ataacgccac ctggccattt acatccacgc cttgagtggg
     3001 ttcatccagc actaataatt gcgggcgatt taacaatgct cgcgctaaca gtacacgctg
     3061 cgtttcgcca ccagagagct tttgcatcgg tgcgttaatc aggtgcccgg cctggacacg
     3121 tttcagtgca ggcaaaatat cttctttatg tgtaccaggg cgtaagcgta aaaaacggtt
     3181 tacggtcagt ggcaacgtgg tgtcgagata cagcttctgc ggtacatagc cgatgcgcag
     3241 ttttccgttg cgcttgataa ccccttcatc gggtgttacc agcccgagca ctacccgtac
     3301 cagtgtcgac ttacctgcgc catttggccc aagtaaagtc aaaatttttc caggtttaag
     3361 ttccagcgac acatcagaga ggacgcggcg ttggccaaaa gaaaccgaga cattttccaa
     3421 ggaaaccaga cttgtcatgt taattttagt cttgcagtag tcatgaaatg ttataatatc
     3481 acacttctca tattcattac gatgattggt cgcattatgt tacataaaaa aacgcttctt
     3541 ttcgcagcat tatccgccgc tctctgggga ggtgcaacac aggccgcaga tgccgccgtt
     3601 gtcgcttcgc ttaaacccgt tgggttcatc gcttctgcca ttgctgatgg ggtaacagaa
     3661 acagaggttt tacttcctga cggcgcttca gaacatgatt attcactgcg cccatcggat
     3721 gtaaaacgct tacagaacgc ggacttagtc gtttgggttg gcccggagat ggaagcgttt
     3781 atgcaaaaac cggtaagcaa attaccagga gcgaagcagg taacgattgc gcagcttgaa
     3841 gatgtgaaac cgctactgat gaaaagtatt cacggcgatg atgatgatca cgaccacgcg
     3901 gaaaaaagtg acgaagatca ccatcacggc gatttcaaca tgcatctttg gctttcccca
     3961 gagatagcgc gggctacagc ggttgcaatc catggaaaat tagtggaact tatgccgcaa
     4021 agtcgagcca aacttgacgc caacctgaag gattttgagg cacaattagc ctcaaccgaa
     4081 acgcaggttg gtaacgagct cgcgccgctc aaggggaaag gttatttcgt ttttcacgat
     4141 gcttacggct atttcgaaaa acagttcgga ctgacaccgc ttggtcattt taccgttaac
     4201 cctgagattc aacctggcgc gcagcgttta catgaaataa gaacacagtt ggttgagcaa
     4261 aaagcaacct gcgtttttgc tgagccacag ttcaggccag cggtcgttga gagcgtcgca
     4321 cgagggacat ccgttcgtat gggaacgttg gatcccctgg ggacgaatat caaactgggt
     4381 aaaacaagct attcagaatt cctgagtcaa ttagccaacc agtatgcgag ctgcctgaaa
     4441 ggagattaat gaggaagtga ttacgtgcaa cagatagccc gctctgtcgc cctggcgttt
     4501 aataatttac cgcgaccaca ccgcgttatg ttggggtcgc tcaccgttct tactctggcc
     4561 gtcgctgtct ggcggcccta tgtttatcac cgcgatgcca cgccaattgt caaaaccatt
     4621 gagctggaac agaacgaaat tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa
     4681 gctgcacaag aagatgaagc cattccccag gacgaactgg atgacaaaat cgccggtgaa
     4741 gcgggcgtgc atgaatatgt tgtttccact ggcgatacgc taagcagcat tctcaatcag
     4801 tatggtattg atatgggtga tatcacccaa ctggctgcgg ccgacaaaga attgcgtaac
     4861 ctgaaaatcg gtcaacaact ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc
     4921 acctgggaag tgtctcgtcg tgaaacccga acctatgacc gtactgccgc taacggtttt
     4981 aaaatgacca gcgaaatgca gcaaggagag tgggttaaca atctgctgaa aggtaccgtc
     5041 ggggggagct ttgttgccag cgccagaaac gccggtttaa ccagcgccga agtgagcgca
     5101 gtgattaaag ccatgcagtg gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt
     5161 gcggtgttaa tgtctcgaga aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc
     5221 gtacgtttgc gttcagaagg taaagattat tacgctatcc gcgctgaaga tggcaaattc
     5281 tacgatcgta acggtactgg tctggcgaaa ggattcttgc gattcccgac ggcgaaacag
     5341 ttccgtatct cgtctaactt taacccgcgt cgtactaatc cggtgaccgg tcgcgttgca
     5401 gcacacagag gtgttgattt cgccatgccg cagggtacgc cagtgctttc agtgggtgac
     5461 ggtgaagtgg tggttgccaa acgcagtggc gcagcaggtt attatgtggc tattcgtcat
     5521 ggtcgcagct acaccacgcg ttatatgcac ttgcgcaaga ttctggtgaa accgggacag
     5581 aaggtgaaac gtggcgaccg tatcgcgctt tccggtaata ccggacgttc aaccgggccg
     5641 catctgcact atgaagtatg gataaaccag caggccgtaa acccgctgac ggcaaaactg
     5701 ccgcgtaccg aagggctgac cggctccgat cgtcgcgaat tcctggcgca ggccaaagag
     5761 attgtgccgc agctacggtt tgattaatta acatccattc gcagccggta cgcagtcagt
     5821 atcggctttt tttatttggt gcggggcaag ttgcgccgct acactatcac cagattgatt
     5881 tttgccttat ccgaaactgg aaaagcatgg aaacgaaaaa aaataatagc gaatacattc
     5941 ctgagtttga taaatccttt cgccacccgc gctactgggg agcatggctg ggtgtagcag
     6001 cgatggcggg tatcgcttta acgccgccaa agttccgtga tcccattctg gcacggctgg
     6061 gacgttttgc cggacgactg ggaaaaagct cacgccgtcg tgcgttaatc aatctgtcgc
     6121 tctgctttcc agaacgtagt gaagctgaac gcgaagcgat tgttgatgag atgtttgcca
     6181 ccgcgccgca agcgatggca atgatggctg agttggcaat acgcggaccg gagaaaattc
     6241 agccgcgcgt tgactggcaa gggctggaga tcatcgaaga gatgaggcgt aataacgaga
     6301 aagttatctt tctggtgccg cacggttggg ccgtcgatat tcctgccatg ctgatggcct
     6361 cgcaagggca gaaaatggca gcgatgttcc ataatcaggg caacccggtt tttgattatg
     6421 tctggaacac ggtgcgtcgt cgctttggcg gtcgtctgca tgcgagaaat gacggtatta
     6481 aaccattcat ccagtcggta cgtcaggggt actggggata ttatttaccc gatcaggatc
     6541 atggcccaga gcacagcgaa tttgtggatt tctttgccac ctataaagcg acgttgcccg
     6601 cgattggtcg tttgatgaaa gtgtgccgtg cgcgcgttgt tccgctgttt ccgatttatg
     6661 atggcaagac gcaccgtctg acgattcagg tgcgcccacc gatggatgat ctgttagagg
     6721 cggatgatca tacgattgcg cggcggatga atgaagaagt cgagattttt gttggtccgc
     6781 gaccagaaca atacacctgg atattaaaat tgctgaaaac tcgcaaaccg ggcgaaatcc
     6841 aaccgtataa gcgtaaagat ctttatccta tcaaataaaa aaagcctctc aaattattga
     6901 gaggctgtac tcgataaaaa atctcaatga gtgttcccac tattttgagt ttttaggtta
     6961 gtacgctatt gagttaatcg tagcgtgata atttcaaaag gtttgaactc aagattgcaa
     7021 tattgattgc aataaaaact gtcatctaac ttattttctt gaagatcgac atattgaacg
     7081 tagtgataat tacatactca cgaaagcatg gctgaaattt ctaatccatg actctcatat
     7141 aatcttacga ttataccgtc gccgtcttca gctaatttta ttgtttcgat gataacgctg
     7201 ttagtatcga tagcaaaaaa tgatagtgaa gggggtaatt cacccgggtg cggtttgaca
     7261 gggacgacct gtaagggatg attcattata taggcttgat taataacacc acccacacgg
     7321 taatctccat gatgaggaaa taaggagaat atgaattttt gtttaccaat atctgcaaaa
     7381 ttattaatca ggaaattatc ataattgtct gtcttaacaa cttccccagg tttatgctgc
     7441 gaacgcagca aagttaattc aagcgtctgg tctttgacac gatagccata tttgtttttt
     7501 gctattaaag ctatgccagt ttctcgatca gaaaggtcga cccagtgatg tgctggtatt
     7561 tcatctttgg caaaagcgaa cgaatcatcg ctatgcgttg gtctgtcaat tacaccgaac
     7621 tgaatttgac attgtgcaag agatgtttgt actgacaccg ggaattttac tttgagagaa
     7681 acattaggtt cgtgccaatc aataaacgtt tggacatcaa cgcgtggact atcctgtgtc
     7741 agggtaaagg ctaaacgaag ataagaggaa ttgtagtgat aaatcagcgt catcgtagtt
     7801 ttaggcccgc tgatcacagt tgtcattttt tcagcgagta aaacatatga ttccccatca
     7861 cgataatttg aagcaaaatc ccaggccgcg aaaaaacctg catcttcgtg gtatgcacgg
     7921 atctcactgt gcatattttc agt
//
LOCUS       DAZPJP010000185         7934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPJP010000185 DAZPJP010000000
VERSION     DAZPJP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.186"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHU9J_003549"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHU9J_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1907005.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHU9J_003550"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHU9J_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1907006.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHU9J_003551"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHU9J_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1907007.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHU9J_003552"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHU9J_003552"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1907008.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHU9J_003553"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHU9J_003553"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1907009.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHU9J_003554"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHU9J_003554"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1907010.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHU9J_003555"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHU9J_003555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1907011.1"
                     /translation="MDKPFLRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHU9J_003556"
     CDS             5315..6439
                     /locus_tag="ACHU9J_003556"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1907012.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PANQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHU9J_003557"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHU9J_003557"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1907013.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>7934
                     /locus_tag="ACHU9J_003558"
     CDS             7245..>7934
                     /locus_tag="ACHU9J_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1907014.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSG"
BASE COUNT         2017 a         1836 c         2029 g         2052 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttctaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcgaca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccaa ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc aggg
//
LOCUS       DAZPJP010000186         7918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPJP010000186 DAZPJP010000000
VERSION     DAZPJP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.187"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHU9J_003559"
                     /pseudo
     CDS             complement(1..738)
                     /gene="dsbG"
                     /locus_tag="ACHU9J_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHU9J_003560"
     CDS             1110..1673
                     /gene="ahpC"
                     /locus_tag="ACHU9J_003560"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1907015.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHU9J_003561"
     CDS             1844..3409
                     /gene="ahpF"
                     /locus_tag="ACHU9J_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1907016.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHU9J_003562"
     CDS             complement(3530..3958)
                     /gene="uspG"
                     /locus_tag="ACHU9J_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1907017.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4179..5417
                     /locus_tag="ACHU9J_003563"
     CDS             4179..5417
                     /locus_tag="ACHU9J_003563"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR1907018.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHU9J_003564"
     CDS             complement(5421..5510)
                     /gene="yldA"
                     /locus_tag="ACHU9J_003564"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1907019.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHU9J_003565"
     CDS             complement(5648..6058)
                     /gene="rnk"
                     /locus_tag="ACHU9J_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1907020.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHU9J_003566"
     CDS             complement(6288..7094)
                     /gene="rna"
                     /locus_tag="ACHU9J_003566"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1907021.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7208..>7918)
                     /gene="citT"
                     /locus_tag="ACHU9J_003567"
     CDS             complement(7208..>7918)
                     /gene="citT"
                     /locus_tag="ACHU9J_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1907022.1"
                     /translation="PWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVL
                     LSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMAN
                     GLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLSVILA
                     VGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGV
                     IYISMLLLVGWPILAMWN"
BASE COUNT         2022 a         1922 c         2029 g         1945 t
ORIGIN      
        1 ataatgatat taagcgtttt ctgatcgggc aaccccacgg cctgttgcag cgtattttcc
       61 ttactcatgt aatagatagc cggcgtgaca tttgccccca gatcgtccat cagtttctca
      121 ttgtcactta acactttcat ttgctctgta cttacgtttg caggcacgtt tagcttaagc
      181 ttgccaccag aggcttcata ttgttgccag gtttttgcgg gatctttgga ggcaagaatt
      241 gccgctgctg tcgccgggct ttctggcttg ataaccccaa ccaacaatgt tcttaattgc
      301 actttgccag aatctaccca cgggcgcgcc tgctgccaga actgtttaca atatgggcag
      361 aacggatcgg cgaagacgta gacaatgacc ggcgcatctt ttttaccgtc gaggatccag
      421 tgggattgtt ccatccgttg ccacatttcg cgtccggctg gtgcgtaaat ttctttttcg
      481 ataagtgtgt tactcaggtt ttcacctttc tcgttgtaca tgtaaccaga gatagcgtgc
      541 ttaccatcgg gagtcaggta ggtagatggt gacgcccata tcctgatact ttccgagata
      601 acctttcatt cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat
      661 cgcttttact ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa
      721 aagtatcttt tttaacatta atttgtcctt ttcagtcagt gcaaaagtcg agtaaaaggc
      781 ataacctatc actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg
      841 gtgttgtaat ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc
      901 gctgaagata ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac
      961 tttttatcgc cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa
     1021 aacttatcga tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac
     1081 atctcatcga aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt
     1141 ttaaaaacca ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag
     1201 gccgctggag cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac
     1261 tgggtgacgt tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag
     1321 tatctaccga tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta
     1381 aaatcaaata tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca
     1441 tgcgtgaaga tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca
     1501 tccaggcaat cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta
     1561 aaatcaaagc agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga
     1621 aagaaggtga agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct
     1681 tcgtctttcg cgctaccaac tccggcgcga aatacttcgg aaattcacct aattcttcgg
     1741 gtgctgcggc acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt
     1801 agaggccgct tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat
     1861 gaaaactcaa ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac
     1921 gctggatgac agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact
     1981 gtcagacaaa gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct
     2041 gatcaccaac ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga
     2101 gttcacctcg ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca
     2161 gtctctgctg gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc
     2221 gctctcttgc cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa
     2281 cccgcgcatc aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg
     2341 caacgtgatg ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat
     2401 gacgttgact gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga
     2461 gctgaacaag cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc
     2521 ggcagcaatt tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg
     2581 tggtcagatc ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg
     2641 gcagaagctg gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga
     2701 cagccagagc gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga
     2761 aacagcttct ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg
     2821 gcgcaacatg aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc
     2881 gcactgcgac ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc
     2941 cggcgtggaa gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt
     3001 tgcgccagaa atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt
     3061 cgacattatt ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg
     3121 tctggaatat cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt
     3181 cgtgcagatt ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg
     3241 catgggtgag attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc
     3301 gggtgactgt acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa
     3361 agcctctttg agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag
     3421 attcacctgc aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa
     3481 aagccctgcc gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga
     3541 ccagtacagg cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg
     3601 tcgaaatcga tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca
     3661 actcattgac ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat
     3721 cgatggtgaa gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct
     3781 cttcaaaacg acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta
     3841 gtacgtgaag tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag
     3901 ctttgtcgct caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa
     3961 ccctttctcc ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga
     4021 tatttgtctg gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac
     4081 accaggaatt ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt
     4141 aaagactgac tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg
     4201 gcccacatca cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata
     4261 ttattctgcg tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca
     4321 aaatacccca ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg
     4381 aaaccggaaa ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca
     4441 ttgcttgtgg cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca
     4501 atgcgggtaa aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg
     4561 gttatagtca cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta
     4621 aagggaatgt ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc
     4681 tttctgatat tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt
     4741 caagcgttgc agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt
     4801 tgctcggtgc ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg
     4861 ctgaccgcta cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa
     4921 ttattgaaca aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg
     4981 aagcgaaagg cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg
     5041 gtaaagcgtt gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg
     5101 gcgtctacgc tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga
     5161 cgtttaaaat gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg
     5221 agaaaggatt actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg
     5281 cccggggata tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac
     5341 ccggtgcaca aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc
     5401 cggggggaac aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata
     5461 atcgccgcca taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc
     5521 acaaacgaaa cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct
     5581 gattttgcgt aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca
     5641 gatgggatta aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt
     5701 caaggtgggt tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc
     5761 ccagcagtgc ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt
     5821 ttgccggata caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa
     5881 cccggctgtt cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc
     5941 gatccaactc tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca
     6001 gcagaatatc gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt
     6061 ttactccatg tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc
     6121 accgtcaggc ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa
     6181 gtgatttgcg tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc
     6241 gtcacggttt atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact
     6301 ttatcaatca caaaggtttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt
     6361 gcagaaagcg gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac
     6421 gcagggttac cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg
     6481 gcaaaggcgg cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat
     6541 tttccggctt cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg
     6601 tccggatcaa aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa
     6661 cgtccgccag ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg
     6721 gtttccggcg atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga
     6781 gtagcgcaac cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta
     6841 ggcaatcctg gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt
     6901 tgtaggcgac attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg
     6961 gtttgccagg agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac
     7021 gctacggcgt tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc
     7081 cagaatgctt tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc
     7141 ataaaaaagc ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc
     7201 gaacgtatta gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt
     7261 aaatcacccc gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc
     7321 cgtaaataat caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag
     7381 acagcaccag caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg
     7441 ccagaataac cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg
     7501 caaagtagaa caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt
     7561 gcgtactcat ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca
     7621 tcacaaccag agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat
     7681 ctttccaagg cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag
     7741 tagcattaat gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa
     7801 tcagtgtcca ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg
     7861 ttgccacttc ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacgg
//
LOCUS       DAZPJP010000187         7833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPJP010000187 DAZPJP010000000
VERSION     DAZPJP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.188"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..644
                     /locus_tag="ACHU9J_003568"
     CDS             <1..644
                     /locus_tag="ACHU9J_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1907023.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            895..2457
                     /gene="hpaB"
                     /locus_tag="ACHU9J_003569"
     CDS             895..2457
                     /gene="hpaB"
                     /locus_tag="ACHU9J_003569"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1907024.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2475..2987
                     /locus_tag="ACHU9J_003570"
     CDS             2475..2987
                     /locus_tag="ACHU9J_003570"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1907025.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3380..5530
                     /gene="btsT"
                     /locus_tag="ACHU9J_003571"
     CDS             3380..5530
                     /gene="btsT"
                     /locus_tag="ACHU9J_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1907026.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5580..5783
                     /locus_tag="ACHU9J_003572"
     CDS             5580..5783
                     /locus_tag="ACHU9J_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1907027.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5794..6750
                     /gene="yjiA"
                     /locus_tag="ACHU9J_003573"
     CDS             5794..6750
                     /gene="yjiA"
                     /locus_tag="ACHU9J_003573"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1907028.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6828..7724)
                     /locus_tag="ACHU9J_003574"
                     /pseudo
     CDS             complement(6828..7724)
                     /locus_tag="ACHU9J_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT         1800 a         2138 c         2056 g         1839 t
ORIGIN      
        1 ctcatgcgtt tattacggag tctgacgccg acggtcatgt gttgacggta cgggaagatc
       61 tgatctggcc cctgctggaa gttctttatc caggcactcg ggaaaccttc ggcctgccgg
      121 ggatttgcct gtcactggca gataaacccg acgaactggc ggcgctggaa cactattggc
      181 aactgataga gcgggaatcg gtggaacaac tgcctggacg ggaacacacc ctgacgctac
      241 tggcacaggc agtgttcacc ctactgctgc gtaatgcaaa actcgacgac catgccgcca
      301 gcgggatgcg cggagaatta aaactgttcc agcgttttaa tatgctgatt gaaagccatt
      361 ttcatcagca ctggacagta ccggattacg ctaacgaact gcatatcacc gaatcacgcc
      421 tcacggacat ctgccgccgc tttgccaacc gtccgccaaa acggttgatt ttcgacaggc
      481 agctgcgaga agccaagcgg ctgctgctgt tttctgataa cgccgtgaac aatattgcct
      541 ggcaactcgg ttttaaggat ccagcttatt ttgcgcgctt ttttaatcgc ttagtcggtt
      601 gctcgcccag tgcttatcgt gccaaaaaag tacctgtgac gtgaaaatcc cttagcctta
      661 acgggaaacc aggcaccacc tgctattccc ctttctccgc ctggagcaag gggatttagt
      721 aacgtcaaaa atcgctaaaa gcgaccgcga tcacaaaacc gtacccgacc tgaaaagtac
      781 cagccattgt cccaaaagtg tcttcccgta atcgcccctg caagcttcaa ttgaataaca
      841 aaaacaacac acaaaattaa caatcgtatt ccgattaata ctgtagaggt cgacatgaaa
      901 ccagaagatt tccgcgccag tacccaacgt ccgttcaccg gggaagagta tctgaaaagc
      961 ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag tgaaagacgt cactactcat
     1021 ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt acgacgcgct gcacaaaccg
     1081 gagatgcagg actctctgtg ctggaacacc gacaccggca gcggcggcta tacccataaa
     1141 ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc aacgtgacgc catcgctgag
     1201 tggtcacgcc tgagctatgg ctggatgggc cgtaccccag actacaaagc tgctttcggt
     1261 tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg agcagaacgc ccgtaactgg
     1321 tacacccgta ttcaggaaac tggcctctac tttaaccacg cgattgttaa cccaccgatc
     1381 gatcgtcatt tgccgaccga taaagtgaaa gacgtttaca tcaagctgga aaaagagact
     1441 gacgccggga ttatcgttag cggtgcgaaa gtggttgcca ccaactcggc gctgactcac
     1501 tacaacatga ttggcttcgg ctcggcacaa gtaatgggcg aaaacccgga cttcgcactg
     1561 atgttcgttg cgccaatgga tgccaatggc gtcaaattaa tctcccgcgc ctcttatgag
     1621 atggtcgcgg gtgctaccgg ctcgccgtac gactacccgc tctccagccg cttcgatgag
     1681 aacgatgcga ttctggtgat ggataacgtg ctgatcccat gggaaaacgt actgatctac
     1741 cgcgatttcg atcgctgccg tcgctggaca atggaaggcg gttttgcccg tatgtatccg
     1801 ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca ttacagcact gctgaaaaaa
     1861 tcactcgaat gtaccggcac cctggagttc cgtggtgtac aggccgatct cggtgaagtg
     1921 gtggcgtggc gcaacacctt ctgggcattg agtgactcga tgtgttctga agcgacgccg
     1981 tgggtcaacg gggcttattt accggatcat gccgcactgc aaacctatcg cgtactggca
     2041 ccaatggcct acgcgaagat caaaaacatt atcgaacgca acgttaccag tggcctgatc
     2101 tatctccctt ccagtgcccg tgacctgaac aatccgcaga tcgaccagta tctggcgaag
     2161 tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca tcaagatcct caaactgatg
     2221 tgggacgcca ttggcagcga gtttggtggt cgccacgaac tgtatgaaat caactactcc
     2281 ggtagccagg atgagattcg cctacagtgt ctgcgccagg cacaaagctc cggcaatatg
     2341 gacaagatga tggcgatggt tgatcgctgc ctgtcggaat acgaccagaa cggctggact
     2401 gtgccgcact tgcacaacaa cgacgatatc aacatgctgg ataagctgct gaaataacgc
     2461 agcaggaggt taagatgcaa ttagatgaac agcgcctgcg ctttcgtgac gcgatggcca
     2521 gcctgtcggc agcggtaaat attatcacca ccgagggcga cgccggacaa tgcgggatta
     2581 cggcaacggc cgtctgctcg gtcacggata caccaccatc gctgatggtg tgcattaacg
     2641 ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa gttgtgcgtc aacgtcctca
     2701 accatgagca ggaactgatg gcacgccact tcgcgggcat gacaggcatg gcgatggaag
     2761 agcgttttag cctctcatgc tggcaaaaag gtccgctggc gcagccggtg ctgaaaggtt
     2821 cgctggccag tcttgaaggt gagatccgcg atgtgcaggc aattggcaca catctggtgt
     2881 atctggtgga gattaaaaac atcatcctca gtgcagaagg tcacggactt atctacttta
     2941 aacgccgttt ccatccggtg atgctggaaa tggaagctgc gatttaagct ccagcccctt
     3001 cacgcattcg tcgaaggggc atttttattg tgatctcaat cacatatcca cccgatgaat
     3061 aattcttgcc caaccgctca ccgccattcc tgaccgctta cgtccctaaa ataaccactc
     3121 agttatttac cttactttac gcgcgcgtaa ctctggcaac atcactgcag gatagcggtc
     3181 aatttacctc ctcaaacgca acgcaaacct agaacggctt cggcctatga ttaacaaatt
     3241 actctatgaa ttttggtttg caggaaggca gcaagtgagt gaatccccgg gagcttacaa
     3301 cagtaagtga caggggtgaa cgaacgcaac tgccgcacct gtaagccaaa agacgacgag
     3361 taaaatgcca ggttttacta tggatacgaa aaaactattc aagcacatac cctgggtgat
     3421 tctcggaatc atcggtgcat tctgcctcgc ggtagttgca ttacgtcggg gggagcacgt
     3481 cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat ctggtggcgt atcgctacta
     3541 cagtctgtac atcgcccaga aggtgatgaa actcgacccc acgcgcgcga cgcctgcggt
     3601 tattaacaac gacggtctga actacgttcc gaccaaccgt tacgtgttgt ttggtcacca
     3661 cttcgccgct atcgccggtg ctggtccgct ggtcggtccg gttctcgccg cacagatggg
     3721 ccacctgcct ggcacgctgt ggctgctggc gggggtagta ctggccggtg cggttcagga
     3781 ctttatggtg ctgtttatct cctctcgccg taacggcgca tctctcggtg agatgataaa
     3841 agaagagatg ggaccagtac cggggactat cgcgctgttt ggctgtttct taatcatgat
     3901 catcatcctt gccgtcctgg cgctgattgt ggttaaagcc ctggccgaaa gtccgtgggg
     3961 tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt atgggtatct acatgcgctt
     4021 tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt atcgtgctgc tggttgcctc
     4081 tatctacttc ggtggcgtga ttgctcacga tccgtactgg ggtccggcac tgacctttaa
     4141 agacaccacc attaccttcg cgctgattgg ctatgcgttt gtttccgcat tgctgccggt
     4201 gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc ctgaaaatcg gcgttatcgt
     4261 cggcctggcg ctgggtatcg tggtgctgaa cccggaactg aaaatgcctg cgatgaccca
     4321 gtacattgac ggtactggcc cgctgtggaa aggtgctctg ttcccgttct tgttcattac
     4381 catcgcctgt ggtgcggtat ctggcttcca cgcactgatc tcttccggta cgacgccgaa
     4441 actgctggct aatgaaaccg acgcgcgttt cattggctac ggcgcaatgc tgatggagtc
     4501 cttcgtggcg attatggcgc tggttgctgc gtccatcatc gaaccgggtc tttacttcgc
     4561 gatgaacacc ccgcctgctg gccttggcat caccatgcct aacctgcatg aaatgggtgg
     4621 cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt accgcacacg cggcagcgac
     4681 cgtcagctct tggggcttcg tgatttcgcc agagcagatc ctgcaaaccg cgaaagacat
     4741 tggtgagcct tctgtcctga accgtgcagg tggtgcgcca acgctggcgg taggtatcgc
     4801 tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc ttctggtatc acttcggtat
     4861 tctgttcgaa gccctgttca tcctgaccgc gctggatgcg ggtacccgtt ctggccgttt
     4921 tatgctgcaa gacctgctgg gtaacttcat cccgttcctg aaaaaaaccg actctctggt
     4981 tgccggtatc atcggtactg cgggctgtgt gggtctgtgg ggctacctgc tgtatcaggg
     5041 cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg ctgttcggta tctccaacca
     5101 gatgctggca gccgtagcgc tggtactggg caccgttgtg ctgattaaga tgaagcgcac
     5161 ccaatacatc tgggtaactg ttgttccggc tgtatggctg cttatctgca ccacctgggc
     5221 gctgggtctg aaactgttca gcaccaaccc gcagatggaa ggcttcttct acatggcaag
     5281 ccagtacaaa gagaagattg ctaacggtac tgacctgacg gcgcagcaga ttgccaacat
     5341 gaaccacatc gttgtgaaca actacaccaa cgcaggcctg agtattctgt tcctgattgt
     5401 ggtgtacagc atcatcttct acggtttcaa aacctggctt gcggtgcgta acagcgacaa
     5461 acgtactgac aaagaaactc cgtacgttcc aatcccggaa ggcggcgtga agatctcttc
     5521 gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt gttcttatca gagtgaacta
     5581 tgtttggtaa cttaggacag gcaaaaaaat atctcggcca ggcggcgaag atgttgattg
     5641 gcattccaga ctacgacaac tacgtcgagc atatgaagac caaccatccc gacaagccgt
     5701 acatgagcta tgaagaattc ttccgcgaac gccagaacgc gcgctacggc ggcgatggta
     5761 aaggcggtat gcgctgttgt taatggagag agcatgaacc cgattgcagt taccctactc
     5821 accggttttt taggcgcggg caaaaccacc ctgctgcgcc atattcttaa cgaacaacac
     5881 ggctacgaga ttgccgtgat tgaaaacgaa ttcggcgaag tctccgttga tgatcaactg
     5941 attggcgacc gcgccacgca gatcaaaacg ctgaccaacg gctgcatctg ctgttcgcgc
     6001 tccaacgagc tggaagacgc actactcgac ctgctggata atctcgacaa gggcaatatt
     6061 cagttcgacc gtctggtcat tgaatgcacc ggcatggccg atcccggccc gattattcag
     6121 accttcttct cccatgagat tttatgccag cgctacctgc tggacggggt gattgcgctg
     6181 gtggatgcgg tacatgccga tgagcagatg aaccagttca ccatcgccca gtcccaggtt
     6241 ggctacgccg accgtattct gctgacgaaa accgacgtcg caggcgaagc agagaaactg
     6301 cgtgaacgcc tggcgcgcat caacgcccgc gcaccggtct acaccgttac ccacggcgat
     6361 atcgacctgg gccttctgtt caacaccaac ggttttatgc tggaagaaaa cgtcgtcagc
     6421 accaaaccgc gtttccactt tatcgcggac aaacaaaacg atatttcgtc gattgtggtg
     6481 gaactggatt acccggtaga tatcagcgaa gtttcccggg tgatggaaaa cctgctgctg
     6541 gaatcggcgg ataaactgct gcgttacaaa gggatgctgt ggattgacgg cgaacctaac
     6601 cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg actgggaccg cccgtggggc
     6661 gatgaaaaac cgcatagcac gatggtgttt atcgggattc agttgccgga ggatgagatt
     6721 cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc aacatcacaa tacctgagac
     6781 tttatcaccc tcatattcct ccagaatatg agggcattcg ggcttaatta tctctgaacg
     6841 tgcaacactt cctgcgactc cttatgcgcc tccacatcct gcttcgttaa ccagagcgac
     6901 ttacgaccaa aattttcgta cattttcagc tgatcttcat agtgctgatc aactgttcca
     6961 tcgggagcaa taaacccact ctgaccgggt gcgaccacat cccaggcaag caccggacga
     7021 tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg ttccacggtt ttgatactcc
     7081 gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac caaagaaatt atttgcacgg
     7141 aacgttaagg ccattgcagg tgttttccag ttactcacat tattgccata gcgtttggaa
     7201 agagtctccc aggtatcttc cagcgcagcc aacacaacct cctgctgtgg tttcccagca
     7261 aacagatcaa ccgcctgtgg gattggtgat ttgtctccct gcaccgcctc atacaaaatt
     7321 tttgctccaa cacttatatt cagcgaacca gttgggccgt cctgggttgt ttcgtagcca
     7381 ctggcgctgt accacttatc aaatggcata ggtacggcag ccactacggt acgcttcaac
     7441 atactggtca gccaaacgtt caggatggca gagcctggct gctgccaggt tttaccatca
     7501 tcattaagca aattgatgcc atcccaacgt gttaatgtat ccaccaactg acggcgcgga
     7561 tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag gtaaaaaaag cctcaggtta
     7621 agatcctgac gactggtttg gcgaataaca tcccatgcct gatcagcagt taagcgtggc
     7681 ttttgctcaa gcagtcggtc gatctccgta acgcgatctg ccccgttact ttattcgtac
     7741 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
     7801 gatccggggg gtgatgtcac cgggtctggt ggg
//
LOCUS       DAZPJP010000188         7797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPJP010000188 DAZPJP010000000
VERSION     DAZPJP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.189"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            261..764
                     /gene="dps"
                     /locus_tag="ACHU9J_003575"
                     /gene_synonym="pexB"
     CDS             261..764
                     /gene="dps"
                     /locus_tag="ACHU9J_003575"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1907029.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1168..1914
                     /gene="glnH"
                     /locus_tag="ACHU9J_003576"
     CDS             1168..1914
                     /gene="glnH"
                     /locus_tag="ACHU9J_003576"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1907030.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2053..2712
                     /gene="glnP"
                     /locus_tag="ACHU9J_003577"
     CDS             2053..2712
                     /gene="glnP"
                     /locus_tag="ACHU9J_003577"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1907031.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2709..3431
                     /gene="glnQ"
                     /locus_tag="ACHU9J_003578"
     CDS             2709..3431
                     /gene="glnQ"
                     /locus_tag="ACHU9J_003578"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1907032.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3548..5773
                     /gene="ybiO"
                     /locus_tag="ACHU9J_003579"
     CDS             3548..5773
                     /gene="ybiO"
                     /locus_tag="ACHU9J_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR1907033.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5770..6696)
                     /gene="rlmF"
                     /locus_tag="ACHU9J_003580"
     CDS             complement(5770..6696)
                     /gene="rlmF"
                     /locus_tag="ACHU9J_003580"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1907034.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6972..7232
                     /gene="mcbA"
                     /locus_tag="ACHU9J_003581"
     CDS             6972..7232
                     /gene="mcbA"
                     /locus_tag="ACHU9J_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1907035.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7497..>7797
                     /locus_tag="ACHU9J_003582"
     CDS             7497..>7797
                     /locus_tag="ACHU9J_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1907036.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1893 a         1989 c         1948 g         1967 t
ORIGIN      
        1 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
       61 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
      121 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
      181 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
      241 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
      301 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
      361 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
      421 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
      481 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
      541 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
      601 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
      661 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
      721 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
      781 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
      841 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
      901 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
      961 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
     1021 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
     1081 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
     1141 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
     1201 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
     1261 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
     1321 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
     1381 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
     1441 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
     1501 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
     1561 aaagtggttg ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
     1621 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
     1681 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
     1741 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
     1801 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
     1861 aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc
     1921 tacacctgta aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg
     1981 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
     2041 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
     2101 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
     2161 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
     2221 gtctttattg aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc
     2281 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
     2341 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt
     2401 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
     2461 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
     2521 atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag
     2581 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
     2641 ttctatctga ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg
     2701 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc
     2761 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
     2821 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
     2881 tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg
     2941 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg
     3001 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
     3061 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
     3121 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
     3181 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
     3241 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
     3301 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
     3361 gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
     3421 acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc
     3481 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
     3541 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
     3601 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
     3661 ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa
     3721 cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc
     3781 ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa
     3841 ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta
     3901 tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct
     3961 gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg
     4021 cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag
     4081 caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact
     4141 ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac
     4201 catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc
     4261 cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga
     4321 cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg
     4381 cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc
     4441 gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa
     4501 aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct
     4561 gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct
     4621 gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt
     4681 gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct
     4741 ggcaaaaacc atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg
     4801 gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc
     4861 ggtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg
     4921 cgcggggcag aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc
     4981 ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg
     5041 ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc
     5101 ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc
     5161 gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct
     5221 ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga
     5281 tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg
     5341 cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc
     5401 taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga
     5461 tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga
     5521 aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac
     5581 cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt
     5641 tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca
     5701 gacttatcag gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga
     5761 gccaacgctt taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt
     5821 ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac
     5881 cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag
     5941 cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat
     6001 aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc
     6061 atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc
     6121 ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat
     6181 gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag
     6241 ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact
     6301 accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc
     6361 aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa
     6421 atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc
     6481 ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt
     6541 caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa
     6601 ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg
     6661 gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg
     6721 taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac
     6781 cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta
     6841 atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt
     6901 tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg
     6961 aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct
     7021 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
     7081 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
     7141 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
     7201 agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
     7261 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata
     7321 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc
     7381 aaaattatta tcacttgcac gaacactatc acgggattaa cagtggcatc gcatccgcag
     7441 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
     7501 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
     7561 gtattggcat cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg
     7621 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
     7681 cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag
     7741 tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAZPJP010000189         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPJP010000189 DAZPJP010000000
VERSION     DAZPJP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.190"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHU9J_003583"
     CDS             complement(<1..1780)
                     /locus_tag="ACHU9J_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR1907037.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHU9J_003584"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHU9J_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1907038.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHU9J_003585"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHU9J_003585"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1907039.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHU9J_003586"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHU9J_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1907040.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHU9J_003587"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHU9J_003587"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1907041.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHU9J_003588"
     CDS             complement(5968..7005)
                     /locus_tag="ACHU9J_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1907042.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHU9J_003589"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHU9J_003589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1907043.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZPJP010000190         7700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPJP010000190 DAZPJP010000000
VERSION     DAZPJP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.191"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(241..987)
                     /gene="cutC"
                     /locus_tag="ACHU9J_003590"
     CDS             complement(241..987)
                     /gene="cutC"
                     /locus_tag="ACHU9J_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1907044.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1001..1567)
                     /locus_tag="ACHU9J_003591"
     CDS             complement(1001..1567)
                     /locus_tag="ACHU9J_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1907045.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1783..3519
                     /gene="argS"
                     /locus_tag="ACHU9J_003592"
     CDS             1783..3519
                     /gene="argS"
                     /locus_tag="ACHU9J_003592"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1907046.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3561..3605)
                     /gene="yecV"
                     /locus_tag="ACHU9J_003593"
     CDS             complement(3561..3605)
                     /gene="yecV"
                     /locus_tag="ACHU9J_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1907047.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3696..4184
                     /locus_tag="ACHU9J_003594"
     CDS             3696..4184
                     /locus_tag="ACHU9J_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1907048.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4304..4696)
                     /gene="flhe"
                     /locus_tag="ACHU9J_003595"
     CDS             complement(4304..4696)
                     /gene="flhe"
                     /locus_tag="ACHU9J_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1907049.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4696..6774)
                     /gene="flhA"
                     /locus_tag="ACHU9J_003596"
     CDS             complement(4696..6774)
                     /gene="flhA"
                     /locus_tag="ACHU9J_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR1907050.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6767..>7700)
                     /gene="flhB"
                     /locus_tag="ACHU9J_003597"
     CDS             complement(6767..>7700)
                     /gene="flhB"
                     /locus_tag="ACHU9J_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR1907051.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1967 a         2079 c         1875 g         1779 t
ORIGIN      
        1 gaataaccca gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata
       61 atcaatgtga atataaaaaa tattattagc acaacgctat gttcactgtc acccgctcaa
      121 cagatcacgg ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga
      181 aattgcaggg gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa
      241 tcatttggcc tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac
      301 gatataacgc gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg
      361 ataacgcatc ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc
      421 atcgaggaag tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc
      481 atcacgatgg gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg
      541 ccctgatgtc agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg
      601 gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat
      661 tattttttcc attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag
      721 tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc
      781 gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac
      841 ccgctggcgc acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc
      901 tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct
      961 gtaacagcaa atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg
     1021 caacgatctc ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg
     1081 ccagcgtcgg attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga
     1141 tccccggtag gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt
     1201 ctggatcacc cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt
     1261 ccccgggcca cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat
     1321 gcaatttaaa cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac
     1381 actgttcaaa cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa
     1441 tgtgatcggc agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg
     1501 cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt
     1561 tcgccataat cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc
     1621 aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc
     1681 gcacgttgct gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg
     1741 tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt
     1801 ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg
     1861 caggttcgtc attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca
     1921 gttgctaaaa aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg
     1981 gatcttaacg gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc
     2041 cttgatccgg cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt
     2101 gtttctacgc cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa
     2161 gagatgcatg tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg
     2221 gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc
     2281 ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag
     2341 ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag
     2401 tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc
     2461 gagatgtggc gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt
     2521 ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg
     2581 ccaggaattg tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc
     2641 gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag
     2701 aagaaagatg gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat
     2761 gaaacactgc atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg
     2821 atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa
     2881 caccacatgt tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt
     2941 ggtacagtga aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg
     3001 gcagaaaaga acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt
     3061 attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac
     3121 tgggacaaca tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg
     3181 cgtgtattgt ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg
     3241 gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa
     3301 accctcaccg tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat
     3361 ctggctggtc tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa
     3421 gaagtgcgta acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt
     3481 ctggatacgc tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa
     3541 gcctgatcag ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg
     3601 gacatgccat tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag
     3661 ggaaacgata acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat
     3721 taggcattat ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca
     3781 tttcagccat caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga
     3841 tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg
     3901 gtgaagctta tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg
     3961 aacaacttaa cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat
     4021 gtgaattaat cctttcaaat gaggacgttc aggatttaag tgacccttat tctgaatcag
     4081 aatggctctc atgtatgatc atacagacca atacgcgaac tcgccagttg cagctatacc
     4141 gtaactctga agatttttat ccaagccctt tgacaagagg ataattcaca tctttttggc
     4201 atgttttgtt gcaagctatt cctgataaat aattgcaaca agacatcgag cctttttcac
     4261 tgagttatta aacatactcg cgagcgcgta atttttttgt ccttcagcga taattcacaa
     4321 tcacttcatt acgttgtacc tttagcggtg gaattaaccg cccaccgcct ggcacttccc
     4381 agataaatcg caacggttct gctgccgcta taccggaaaa ggccacggtg gttccgctct
     4441 gcccctctaa ttcgacacaa cgagattgcg aacacaagcg aacccgcagt cctgaaggtg
     4501 tcgggccgat aagctgataa cgccacgcta ccagcgtcat caatcctgaa gcaggttgtc
     4561 gcgtagaaag aggcgcagac gacatcgact caccgcgatg atttagcgta attcctacac
     4621 tacttgcctg ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatattg
     4681 ctaataaggt tctcattatt tgccgccaat tgtcgccgtc atgcggatat gtcggttatc
     4741 agacagttcc agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga
     4801 caataatggt cgcagcgcgt ggttcaccaa caatactggc ggcgcgccca gcatctcctg
     4861 acgggatagc gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc
     4921 tcccccgccc tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac
     4981 atggacttca tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg
     5041 cacgacggcg gttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc
     5101 gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt
     5161 atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc
     5221 ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg
     5281 ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta
     5341 cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa
     5401 tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc
     5461 cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg
     5521 atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc
     5581 catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg
     5641 ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag aatcttccag
     5701 ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt
     5761 gggttcggca ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc cgagcaatcc
     5821 ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag
     5881 cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc
     5941 gccaacatcc tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg tagaaatcac
     6001 cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc
     6061 cgcgtgtccc atgctcatgc catgttgcag cacgccgacc agcaacccgc cgacaacgtt
     6121 aatgaccatg atgaggatcc cggcgatggc atcgccgcga acaaacttac ttgccccgtc
     6181 cattgagccg taaaaatcgg cttcctgagt cacttcggag cggcgttttt tcgcctcatc
     6241 ttcaccaatc aatccagcgt taaggtcggc gtcaatcgcc atctgcttac ccggcatacc
     6301 atcgagaaca aagcgcgcac ccacttctgc gatacgcccg gcacctttgg taatgaccat
     6361 aaagttgatg atcacgagaa tgacaaacac cacgataccg atagcgaaat tgccaccaac
     6421 gaggaagtga ccgaacgctt cgaccacctt ccctgccgcc gccgcgccgg tatgcccttc
     6481 catcaaaatg atacgggttg aagccacgtt aagtgccaga cgcaacaacg tagtaaacaa
     6541 cagaatggtc ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg ccaccagcaa
     6601 caccatgatc gacaaggcaa tattgaaggt aaacaacagg tcgagtatga atgcgggcag
     6661 tggcagcacc atcatcgaca agatcaacag gatcaaaatc ggtccggcaa ggatctgcca
     6721 ttgtgtcgat ttcaggtttg cgggcaggcg cagcatcgcg gccagattac tcatgggtcg
     6781 gtttctcgtt aataaaatcc agggcttccg gcaccggaag atgagtaggt tgtacagggc
     6841 gctgtccacc agccagacgc cagcgtttca gttgccagac ccaggccagc acttccgcca
     6901 ccgcggcgta cagttgaccc gggatttgtt gaccaatctc cgcatgtcga tacagcgctc
     6961 gcgccagcgg cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc
     7021 gcagcgcgac cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt
     7081 catactgcaa cgctaccgaa tagtgggtcg ggttattgac aatgacatca gctttcggca
     7141 catcggccat catccgacgc cgtgcagcag ctcgctgcat ctgacggatc cgccctttaa
     7201 catgagggtc accttcgctt tgtttgaact catcacgaat atcctgccgc gacatacgca
     7261 gctttttcag gtggctgaag atttggaaaa agacgtcaaa tcccaccatt ggaatgacac
     7321 caagcaccac cagcagtgcg catagcccaa ccagatccat cgcattaccc atggcggtag
     7381 tcggagactc ggccattaag cgcatcatct gcggccagtg atgccagaga taaaaccccg
     7441 tcacgctgcc aaccaggatg gttttcaaaa ttgccttaag caactccgcg ccagtctgtg
     7501 ccgagaacat ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg
     7561 atttgccgct aaataccagc ccccccagca tgaccggaga aatgagcgcc accagcacca
     7621 cgccgctaat cagcggcagc agcgccagca tggcttctct gatcagcaga ataatctgcc
     7681 cgaggatcag attcgggtca
//
LOCUS       DAZPJP010000191         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPJP010000191 DAZPJP010000000
VERSION     DAZPJP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.192"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHU9J_003598"
     CDS             171..701
                     /locus_tag="ACHU9J_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1907052.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHU9J_003599"
     CDS             837..1502
                     /locus_tag="ACHU9J_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907053.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHU9J_003600"
     CDS             1824..2447
                     /locus_tag="ACHU9J_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR1907054.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHU9J_003601"
     CDS             2532..2750
                     /locus_tag="ACHU9J_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1907055.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHU9J_003602"
     CDS             2728..3798
                     /locus_tag="ACHU9J_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1907056.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHU9J_003603"
     CDS             3933..5216
                     /locus_tag="ACHU9J_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1907057.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHU9J_003604"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHU9J_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1907058.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZPJP010000192         7605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPJP010000192 DAZPJP010000000
VERSION     DAZPJP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.193"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(565..941)
                     /gene="rnpB"
                     /locus_tag="ACHU9J_003605"
     ncRNA           complement(565..941)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHU9J_003605"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(974..2119)
                     /gene="garK"
                     /locus_tag="ACHU9J_003606"
     CDS             complement(974..2119)
                     /gene="garK"
                     /locus_tag="ACHU9J_003606"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1907059.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2216..3106)
                     /gene="garR"
                     /locus_tag="ACHU9J_003607"
     CDS             complement(2216..3106)
                     /gene="garR"
                     /locus_tag="ACHU9J_003607"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1907060.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3136..3906)
                     /gene="garL"
                     /locus_tag="ACHU9J_003608"
     CDS             complement(3136..3906)
                     /gene="garL"
                     /locus_tag="ACHU9J_003608"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1907061.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3922..5256)
                     /gene="garP"
                     /locus_tag="ACHU9J_003609"
     CDS             complement(3922..5256)
                     /gene="garP"
                     /locus_tag="ACHU9J_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1907062.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5631..7202
                     /gene="garD"
                     /locus_tag="ACHU9J_003610"
     CDS             5631..7202
                     /gene="garD"
                     /locus_tag="ACHU9J_003610"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1907063.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7351..>7605
                     /gene="prlF"
                     /locus_tag="ACHU9J_003611"
     CDS             7351..>7605
                     /gene="prlF"
                     /locus_tag="ACHU9J_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR1907064.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTR"
BASE COUNT         1890 a         2067 c         1855 g         1793 t
ORIGIN      
        1 catcttaccc tgtgcaccaa aagcaaaaac tcactgacgc gggtttgaaa ctgatggcaa
       61 ccgcaaaaat gcctgatgcg ctacgcttat caggcctacg ccatctctac aatatattga
      121 atttgcgtgc ttttgtaggc aggataaggc gttcacgcag catccggcat gaataaagcg
      181 cactcaacaa tctgaaaccc gccggaacgg tttattacgt acatcaggtg aaactgaccg
      241 ataagccgct ttcttttggg tatagtgtcg tggacagtca ttcatctttc tgcccctcca
      301 aaagcaaaaa cccgccgaag cgggttttta cgtaaatcag gtgaaactga ccgataagcc
      361 gctttctttt gggtatagcg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa
      421 aaacccgccg aagcggtttt ttacgtaaac caggtgaaac tgaccgataa gccgctttct
      481 tttgggtata gcgtcgtgga cagtcattca tctttccgct cctccaaaag caaaaacccg
      541 ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt tctgtcgtgg
      601 acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt
      661 acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac
      721 ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg
      781 cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg
      841 cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc
      901 gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt
      961 ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac
     1021 gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc
     1081 tggtcaatac gctgaagacc gcatcgatgc catgctgatg tacaacgcca acatcatcgg
     1141 tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcaacgc
     1201 caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca
     1261 gcgtacaatc gtgaatatgt tcttccagat tcagcgccgt agtgacgatt tcaataccac
     1321 ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc gcagctcctg
     1381 cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacatcg gcatagtgag
     1441 agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga
     1501 tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga
     1561 cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac
     1621 taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct
     1681 gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata ataatgtttg
     1741 ttgcactgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggttacga
     1801 gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa
     1861 acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg cccagcggcc
     1921 ctgtaaccca ggcgtgacgt tcagccccct gggtggctgc aatcatcgct tccaccgttc
     1981 cttcaccacc gtcggcaacc ggaacagaaa catactgtgc atcaggaaaa atttcccgaa
     2041 atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg
     2101 gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct
     2161 tattcggcct agattccagg cccggattgc aatacaccat ccgggcacga cgtcattaac
     2221 gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagggcgcta tgatccgccg
     2281 ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct gtgagcagca
     2341 gttgtgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta atatgcagat
     2401 caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca
     2461 cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg ttaacgcccg
     2521 ctttggttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc acaatgacct
     2581 gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc acggaacccg
     2641 ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca
     2701 ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat
     2761 caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac
     2821 tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg
     2881 ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag
     2941 cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca gcaatagctt
     3001 ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg
     3061 gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca accatctctc
     3121 ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg cagagcggaa
     3181 gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc
     3241 atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcgctgg cacggttaaa
     3301 aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat ggcccaatgc
     3361 cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag cggcaatagc
     3421 atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtaa tgttcttgtt
     3481 cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac
     3541 gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg cttcctcttt
     3601 tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat
     3661 taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt tcagggccat
     3721 taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca
     3781 gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca
     3841 accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt
     3901 attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac
     3961 gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt
     4021 gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac
     4081 attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc
     4141 agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag
     4201 catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg aaaccagcaa
     4261 cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag
     4321 ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga
     4381 ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa
     4441 ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa
     4501 catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc
     4561 gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag
     4621 ctcttccgca gacatacgtg ggtgatctgt cgggttatga atcaacttga tccacagcgc
     4681 cgtcagcaca aaaccaatcc cccccataac ggtgaagacg tgctcccagc cccaagcgaa
     4741 agtcagccag ccaagcagcg gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
     4801 gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt
     4861 cgccgggaat gatggcgctt ccgagaaccc cagcataaag cgcataaaga acatggagat
     4921 ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga acagcgacca
     4981 gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca accagccgcc
     5041 ggggatttgc atcagcaagt aggcccagcc aaaagcagag aagatgtaac ccatcgaaac
     5101 cgcacttaac tgcaactctt ttgccacttc ggtaccagca atagacagcg ttgcacgatc
     5161 ggcgtagtta acggcggtaa caataaaaat aatcagtaat attaaatagc tggtatgcac
     5221 gcctttcttt tttacgtcaa cggtgtccag aatcatttta tttacctcgg gtacttatgc
     5281 tgatttttat tattatgggg aaggtgttat ttatgagttt tatttatgcc gttacgacaa
     5341 tgaactcggg aattagtata agcagcggga gaataataat cattgtgcaa atgctaattt
     5401 aatcagtact atttaaatat tattttgagc atatgcacat aaggttgcgc gctaaagcac
     5461 agatttgcgc tttaccttac cgggcggcac tgcaatccct gaaatgattg acattgatca
     5521 catttctgcg tttaaactcc tgacattctt atttcaccca atgaagtcat ttatttttaa
     5581 atgagaccag atcctcattt taataacccc tggctggaga atattgcaca atggccaaca
     5641 tcgaaatcag acaagaaacg ccaactgcgt tttatataaa agttcacgac acagataatg
     5701 tggcaattat tgttaatgat aatggcctga aagcaggaac gcgttttccg gatgggctgg
     5761 aattaattga acatattccc caggggcata aagtcgcatt gctggacatt ccagctaatg
     5821 gtgaaattat tcgttatggc gaagtgattg gttacgccgt gcgcgcaatc ccacgcggaa
     5881 gctggatcga cgaatcaatg gttgtactac cggaagcgcc gccgttacac acgctgccac
     5941 tggcaaccaa agtcccggcc cccttaccgc cgctggaagg atacaccttt gagggctatc
     6001 gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg catcaccacc agcgtccact
     6061 gtgtggcagg cgtggtggac tatgtagtaa aaatcattga acgcgatctg ctaccgaaat
     6121 acccgaacgt cgatggcgtg gtggggctga atcatttgta cggctgtggc gtggcgatta
     6181 acgcaccggc ggcagttgtg cctattcgta ccatccacaa tatttcgctg aaccctaact
     6241 ttggcggcga agtaatggtg attggtctgg gttgtgaaaa gttgcagcct gagcgcctgc
     6301 tggtcggcac ggatgatgtg caagctattc cagtagaaag cgccagcatt gtcagtttgc
     6361 aggatgaaaa gcatgtcggc tttcagtcca tggtcgagga tattttgcag gtcgccgaac
     6421 gccatctaca aaaactgaat caacggcagc gagaaacctg cccggcttca gaactggtcg
     6481 ttggtgtgca gtgcggtggc agcgatgcgt tttctggtgt aacggcaaac ccggcggttg
     6541 gctatgcgtc tgatctactg gtgcgctgcg gcgcaacggt gatgttttca gaagtaacgg
     6601 aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt gaacgaagag gtcggcaaac
     6661 ggctgctgga ggagatggag tggtacgata actatctcaa tatgggaaaa accgaccgca
     6721 gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc aaatgtggtg gagaaggcac
     6781 tcggctccat tgctaaatcg ggtaaaagtg caattgttga agtgctgtcg cccggtcaac
     6841 gcccgactaa acgcggatta atttacgccg cgacgccagc cagcgatttt gtctgtggca
     6901 cgcaacaggt ggcttcgggt atcaccgtac aagtgtttac gaccggccgt ggtacgccgt
     6961 acggcctgat ggcggtaccc gtaattaaaa tggcaacccg caccgagctg gcgaaccgct
     7021 ggtttgattt aatggatatt aacgcgggca ctatcgctac cggcgaagag agcattgaag
     7081 aggtgggctg gaagttgttc cactttattc tcgacgtcgc cagcgggaag aagaaaacct
     7141 tctcggatca atgggggctg cataaccagc tggcggtgtt taacccggca ccggtgacct
     7201 gatttctttt ggaacgtccc tcgcaaaaca tggccttagt gccatgtttt tattgtttaa
     7261 agcccccacg tccattaata atgcatttgc attaccttag ttcaagctta taatttgagc
     7321 agaaaacagg ctgtaaaagg acaatgaatc atgcccgcta atgctcgctc tcacgctgta
     7381 ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa ctatccccgc gccagtgcgt
     7441 gaggccttaa aactgaagcc aggccaggac agcattcatt acgaaattct gcctggtggg
     7501 caagtattta tgtgccgact gggagatgaa caggaggatc atactatgaa tgcatttttg
     7561 cgttttctgg atacagatat ccagaacaac ccgcaaaaaa ctcgt
//
LOCUS       DAZPJP010000193         7592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPJP010000193 DAZPJP010000000
VERSION     DAZPJP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.194"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            28..114
                     /locus_tag="ACHU9J_003612"
                     /pseudo
     CDS             28..114
                     /locus_tag="ACHU9J_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            468..710
                     /gene="yiiF"
                     /locus_tag="ACHU9J_003613"
     CDS             468..710
                     /gene="yiiF"
                     /locus_tag="ACHU9J_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1907065.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1040..1969)
                     /gene="fdhE"
                     /locus_tag="ACHU9J_003614"
     CDS             complement(1040..1969)
                     /gene="fdhE"
                     /locus_tag="ACHU9J_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1907066.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1966..2601)
                     /gene="fdoI"
                     /locus_tag="ACHU9J_003615"
     CDS             complement(1966..2601)
                     /gene="fdoI"
                     /locus_tag="ACHU9J_003615"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1907067.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2598..3500)
                     /gene="fdoH"
                     /locus_tag="ACHU9J_003616"
     CDS             complement(2598..3500)
                     /gene="fdoH"
                     /locus_tag="ACHU9J_003616"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1907068.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3513..6563)
                     /gene="fdnG"
                     /locus_tag="ACHU9J_003617"
     CDS             complement(3513..6563)
                     /gene="fdnG"
                     /locus_tag="ACHU9J_003617"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5976..5978),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1907069.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6757..7590
                     /gene="fdhD"
                     /locus_tag="ACHU9J_003618"
     CDS             6757..7590
                     /gene="fdhD"
                     /locus_tag="ACHU9J_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1907070.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1734 a         1994 c         2019 g         1845 t
ORIGIN      
        1 ctcttaaacc gtgggaagga ggctataatg gttgccaata ttaatctcat taaacaagaa
       61 agttattctg ttgttaattt agaaaaacaa ctttcccgaa ctgttaccaa cgaaattata
      121 acgatgagca agtaaagacc ggttggtggc agtactgacc ggtctgtgat gaaagattaa
      181 tttcacctta aatcctggtg ctgttaggat gctgacgttt ttcggctaca ccattcagat
      241 tatgcaaata aatatccctg aatataatat attcatagca cccataactc atcctgggcg
      301 ataactaaac catttttgag taataaatga aacaactgca acgctcttat tatttctgga
      361 aaacgcctcg caaaaataaa aaatgatgcg taaatgagct ttctttaaag attcggcaag
      421 ggtacttttc tctccataga ttcaaaaatg atattggcga gatatttatg aactctctgg
      481 caggtataga tatgggcaga attttactcg atttatcgaa tgaggtgatt aagcaacttg
      541 atgatctcga ggtgcagcgt aatcttcctc gtgcggacct attaagggaa gcggtagatc
      601 aatacctgat aaatcaatcg caaacagcaa gaaccagtgt tcctggcatc tggcaagggt
      661 gtgaggaaga tggtgtcgaa tatcagcgta agctgcgcga ggaatggtaa tgggaaaaaa
      721 tgatgtcaat cagattgctg acaacgtgcg cgttgttcat gccggatgcg gcgtgaacgc
      781 cttatccggc ctacaaagtc atgcaaattc aatatattga agtttattgg taggcctgat
      841 aagcgcagcg catcaggcaa tcatacgctt gtcatcagtg acatacccat aaccttatcg
      901 atgtgttgga gggaagaacg aggcaaacag acaattgcgg catgatcttg aatgggtaac
      961 gcggaatacg agagatggtg tcgtcacacc ctatgagctg taatccgggc gacctgcgcc
     1021 acccggaaaa cgtcagagat tactctcctt cacccggaaa caggaacggg ttaatggaac
     1081 tacgggcgta gccttcttgc tccattcgcg cgtccagtac cagagaggcg aggtcatctg
     1141 ccacggcttc aactttcggt tctttttcct gataaagaat cttcaggtaa gtgccgcagt
     1201 catcgcagct ttcggcttta atcgcggcct gttcgtcatc cagcgaccag taatgcagtt
     1261 tgccgctctg ctcgcagttg ctgcatttta cgcgcactac gtgccattcg gtttcacaca
     1321 ggttgcagtg caggtaacgc agaccctgag tggtgccaat ttgcaccatg ctggacaccg
     1381 gcatagagcc acataccggg caatattgac gttgttcgcc gtattcagcg cgggctttgc
     1441 cggggatcag attggccatc tgcgcccagt agagcgacag cgcagcccag ataaacggcg
     1501 ctttatcgct gctgacggac gagaaatcag aggcaaacag cgcgctggcc atatcttcca
     1561 gctcctgagt cgatgccttc tccagattct caatcactgc cagcgccggg ccgctcattt
     1621 caggtttcag ctcagcaatt agcgccatca gcagcttttg ccagtgctta tcacgcggca
     1681 gaacgtgaat atccagcggg ggcttgccct gtgcgctggc ttctttaatg cgcgcggtca
     1741 gatccatctc cagcggatgg tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag
     1801 caaagcgcag gtaatcaccc agcggattat tttctgccag ctcgcgcaga cgctcggcgc
     1861 ggcggttgta taaattcttg agccgaggga acaataacgg cggaatcata tccgccgtac
     1921 gtttctcgct cgaacccagc tcatcttgcg ggattatgcg aatactcatt cagctttctt
     1981 ttccgttgtc ttgcggacct cacggtacca gcgcgggtga tgtttcttcg cccaggcgct
     2041 ggtaacccat ccttccacca tggcggtaat cgtgcctttc acccaaaggg cggcgtagat
     2101 atgcaccatg ataaccacaa ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa
     2161 tcggatcacc gggattgaga aagcaggcgc aaaataagga cgccagataa tcacgccgct
     2221 caccagcagc agaaccagga aaataatcgc cgcccagaaa acgcatttct gaccgaagtt
     2281 ataacgcccg gtgtcaccta cttcctcgtt gacgacgatc ttacgaatat tcttcgccca
     2341 aaagatatca tcccgattga ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa
     2401 cgaggcaaac ataaccacgc cgacaaacgg gtgcagaatt cgcgccagct gcggtgtgcc
     2461 catgatttgc atcaaccagt tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc
     2521 caggatgaag cagaaggcgg tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac
     2581 gatggtgtca cgtcgtttca tttgcgctcc tcgtctttct cttcgtgcag attattctct
     2641 tcctcatccg cacggttcgg accgacaccc acgtagtgga agatactggc cgcaaaggta
     2701 gccgcaaagc caacagccgc gagcggtttc cagatgcctt tccagaattt cacggtttcg
     2761 ctgatttccg ggttctccgg caagccatga tacagatttg gcttgtcagc atggtgcagc
     2821 acgtacatga cgtgtgtacc accgacgcct gccggatcgt acagacccgc attgtcgtaa
     2881 ccgcgggttt tcagctcagc aacgcgctcg ctcgccagcg ttttcatcga ctctttcgta
     2941 ccgaagtgaa tcgcgcccgt tgggcaggtc ttcacgcagg ctggttcttg cccaaccacc
     3001 acgcggtcaa cgcacagcgt acatttgtag acgcggttgt cttccgggtt gaggcgcgga
     3061 atgtcgaacg gacagcccgc aatgcaataa ccgcagccaa tgcactgctc ggactggaag
     3121 tcgacgatac cgttggcata ctgaataatt gcaccttccg ccgggcacgc tttcaggcag
     3181 cctggatcgg aacagtgcat acagccgtct ttgcggatca gccattccag tttgtcgttc
     3241 tgctccactt ccgagaagcg catcaccgtc cacgatttgg cgcttaaatc attggggttg
     3301 tcgtacaccc caatgttatt gccgacggta tcgcgaatgt cgttccactc tgaacacgcc
     3361 acctgacagg ctttacagcc gatacaggtg gtgacgtcga tgagtttcgc cacttcttcc
     3421 tggaagtccc gcgcctgagg cgcgggggtc agaccgttag tcgcggaacg acgaatgata
     3481 tcttgcgatt gataagccat atgtcgtctc cgttacacct tttccacatt cacaaggaag
     3541 gacttaaact ccggcgtctg cgtgttcgca tcaccaacga atggcgttaa cgtattggca
     3601 ataaagcctt ttttagctac gccttcatag ccccagtgaa tcggaatacc gatggtatcg
     3661 atatctttgc cgttcgcttt cagcgtgcga atacgtttgg tcaccaccgc tttggctttg
     3721 atatagccac ggttggagga gactttcacg gtatcgccct gggcaatgcc aagtttattc
     3781 gccagcgact ccccgatttc cacaaactgc tctggttgca aaatcgcgtt caacagcgcg
     3841 tgtttggtcc agtagtggaa gtgctcggtc agacgatagg tggttccgac atacgggaac
     3901 ttatcggctt tacccaatgc ttcggcgtcg tctttaaaga ttcgcgcagc cgggttcgag
     3961 ataacgtttg gatgcagcgg gttagttccc agcggcgttt caaacggctc gtagtgttcc
     4021 gggaacggac cttccgccat cttatcgagg gcaaacagac gccccatgcc ttcctgctgc
     4081 atgataaacg gcccgacgcc gctgcccgga ggcgctgcgc tgtagtccgg aatatcccag
     4141 ccggtccact tagtgccgtc ccatttcagc aactgacgct tcggatccca cgggttaccc
     4201 tgcggatctg cggaggcgcg gttatacaga atgcggcggt taagcggcca tgcccatgcc
     4261 cagcccagcg tgttaccgag gccagacgga tcggcgttat cacgacgcgc catctggttg
     4321 ccttccggcg tccagctacc ggcgaaaatc cagcagccac aggaggttgt accgtcatcg
     4381 cgcaattggg cgaacgagct aagttgttgg cctttcttga cgataaccgc accggttgcc
     4441 ggatcggtaa tatcggccag cgccttaccg ttgctctcca tcgccacttc ttccgacttc
     4501 ggctcatgcg gaatggcgta gttccaggtc atgttcagca cctgatccgg gtttgcgcca
     4561 ccctgttcgg catacatctt gcgcaagcgc aggaagatac cggagaggat ctcgccatca
     4621 gtcagcgcaa tccctggggc gtccgcacct ttccagtgcc actgcaacca gcgacctgag
     4681 ttaacgattg aaccgttctc ttctgcgaag caggtcgatg gcagacggaa cacttcggtc
     4741 tggatcttcg acgagtcaac ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt
     4801 tcagtgttca gcgggtcgat ggtgacgagg aacttcagtt tcgacagaca accgatcact
     4861 ttgtttttgt tcgggaatga ggcaacaggg ttaaagccct ggcagatata gccattgacc
     4921 ttgccctctt tcatcatctc gaagtattgc aggacgtcgt agcctttatc ccacttcggc
     4981 aaccagtcaa agccccagct attttccgcc gtcgctttat caccaaagaa ggccttcatc
     5041 atagagacga agaatttcgg gtagttgccc cagtagttta cctggccttc cagcagcggt
     5101 tttggcgtgt tggcggtaag gtaggtttgc agatcggtct gcttctcgct tggcagcgtc
     5161 atgtaacctg gcaggctctg cgacagcagc cccaggtccg tcagcccctg aatattggag
     5221 tgaccgcgca gggcgttaac gccgccgcct gccatcccca tattgccgag cagcagctgg
     5281 atcatcgcca tcgtacgaat gttttgcgca ccaacggagt gttgcgtcca gccgagggca
     5341 tacaggaacg aggcagtttt atcgtgagca ctggtttctg cgatgtattc gcagactttc
     5401 aggaacgcgt cttttggcgt accacagatg ttttcaacca catctggcgt gtagcgggaa
     5461 acgtgctgtt tcagcaagtt ccacacgcag cgcggatgtt gcagcgtggt atcgcgtttg
     5521 gcgaagccgt tttcgtccag ttcgtaagtc caggaggatt tatcgtactt gcgtttttcc
     5581 gcgtcatagc cggtgaacag gccatcttca aagccgtaat cctcacgcac gatcaggctg
     5641 gcgttggtat aggcttcggt gtattcgcgg ttgaattttt cattgttcag caggtacagc
     5701 aatacgcctg acaggaaagc aatgtcagta ccggaacgaa taggggcata gtagtcagcc
     5761 accgccgccg tacgcgtaaa gcgaggatcg atcacaatca gcttcgcgcc attgtgaatt
     5821 ttggcttcca tcgcccagcg gaacccgacc gggtgagctt cagcggcgtt accgcccatc
     5881 accacgacga ggttggcgtt cttgatgtcg acccagtggt tggtcatcgc accgcgacca
     5941 aatgttggag caagacttgc taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc
     6001 gcgagcatac ccagcgcgcg ggagaatttt tgcgttaaat agccggtttc gttactcgac
     6061 gcggaagcac acagcatccc ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt
     6121 tgcgcaatgt agttagcatc gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc
     6181 tcttcccaac tgatttgctg ccatttatca gaacctggcg cacggtattc cggaaacttc
     6241 agacggcttt cggagtggat gaaatccacc aggccagcgc ctttcggaca aagtgcaccg
     6301 cggttgaccg ggtgatccgg atcgccttcg atatggaaga tagatgcttt ggcgtttttt
     6361 gctccgtcac cgaggctgta catcaacagc ccacagccta cggaacaata ggtgcaggta
     6421 ttacgggttt cgcgggtgcg cagcagttta tactgccggg tttccgcgag tgctacgctg
     6481 ggtgcaaaac ccagtgccgc tgccgtggtg cctgccatac cgccagcgca gatcttaaag
     6541 aactgccttc tgctgacctg catggattgc tccttgtttc gacattgtca cgtcccattt
     6601 acattcgctt gctgcgtgtg cagggagtgg gagttatttt tctttgcgga aggggccgca
     6661 aaggtccaga attggctcaa tttccctcca tccaggaagg gtttgtaaca gaataccata
     6721 atgttggtgt gtgtgttctt atctggttaa gagaaagtga aaaaaacaca gcgaaaagaa
     6781 atcgaaaatg tgacaaatat cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat
     6841 ttacaacacc cacggttaga tgaggtcgcg gaagaagttc ccgttgcgct ggtctacaac
     6901 ggcatttcgc atgtggtgat gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt
     6961 ttttcgcttt ccgaggggat aatagaaagt ccgcgcgata tcttcggcat ggatgtcgtt
     7021 ccttcctgta atggtcttga agtacaaatt gagctttcca gccgccgctt tatggggttg
     7081 aaggagcgcc gccgggcgct ggcgggacgt acgggctgtg gcgtatgcgg cgtggagcaa
     7141 cttaatgaca tcggaaaacc ggtgcagccg ctaccgttca cccagacgtt tgatctcaac
     7201 aaactggatg atgcattacg tcatctcaac gattttcagc cagtggggca actgactggt
     7261 tgtactcacg ccgctgcctg gatgttgcca tctggcgaac tggtcggcgg gcatgaagac
     7321 gtgggtcgcc atgtggcgct ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc
     7381 tggcagcaag gtgcggtact ggtttccagt cgtgccagtt atgaaatggt gcaaaagtcg
     7441 gcgatgtgcg gcgtagagat tttgtttgcg gtgtctgccg cgaccacgct tgctgtagaa
     7501 gtggctgagc gctgtaatct gacactggta ggtttttgta aaccgggtcg ggcaacggtt
     7561 tatacccatc cgcagcgttt gagcaattaa tt
//
LOCUS       DAZPJP010000194         7590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPJP010000194 DAZPJP010000000
VERSION     DAZPJP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.195"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            79..1368
                     /gene="hpaG"
                     /locus_tag="ACHU9J_003619"
     CDS             79..1368
                     /gene="hpaG"
                     /locus_tag="ACHU9J_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1907071.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHU9J_003620"
     CDS             1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHU9J_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1907072.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHU9J_003621"
     CDS             2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHU9J_003621"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1907073.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3694..4074
                     /locus_tag="ACHU9J_003622"
     CDS             3694..4074
                     /locus_tag="ACHU9J_003622"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1907074.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4185..4988
                     /gene="hpaH"
                     /locus_tag="ACHU9J_003623"
     CDS             4185..4988
                     /gene="hpaH"
                     /locus_tag="ACHU9J_003623"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1907075.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4999..5787
                     /gene="hpaI"
                     /locus_tag="ACHU9J_003624"
     CDS             4999..5787
                     /gene="hpaI"
                     /locus_tag="ACHU9J_003624"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1907076.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5962..7338
                     /gene="hpaX"
                     /locus_tag="ACHU9J_003625"
     CDS             5962..7338
                     /gene="hpaX"
                     /locus_tag="ACHU9J_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1907077.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7348..>7590
                     /locus_tag="ACHU9J_003626"
     CDS             7348..>7590
                     /locus_tag="ACHU9J_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1907078.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1745 a         2128 c         2152 g         1565 t
ORIGIN      
        1 ttgccaaact acgcaatcgg gaataaaagc aatcattaac atattaatga ttaagatgat
       61 cgaatccgag gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc
      121 caacttgatg catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact
      181 gcggtctggt ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt
      241 ccacagggtg aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg
      301 acgaaagtac gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac
      361 gtcagcctgc cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga
      421 ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc
      481 gagatcaacg ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg
      541 cagttgctga gcgccctgag cgaatttgcc acgctgaatc caggcgacgc cattctgctc
      601 ggcacgccac aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa
      661 ggtttcccgc cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcacaagagc
      721 ttcccaacgc agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac
      781 cacgccagcg aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca
      841 aataccctca ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac
      901 tatgaagcgg agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat
      961 gccatggatt atgtcgcggg ctacaccgtg tgtaacgact acgccattcg tgactatctg
     1021 gaaaactact accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt
     1081 tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc
     1141 gtcaacggcg agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc
     1201 ctgatcgcct acttaagtga atttatgacc ctgaatccgg gcgacatgat cgccaccggc
     1261 acacccaaag gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc
     1321 gtgggccgcc tggtgaaccg aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc
     1381 attggatcaa cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa
     1441 cgggtgaagt gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag
     1501 cggcagcgaa agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc
     1561 tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa
     1621 ccgcggacac cggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttccc
     1681 acaactttga atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg
     1741 acgacaagat gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc
     1801 cgtggaacgt accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca
     1861 ataccgcggt actgaaaatg tcggaactct ccccgctgac cgctgacagc ctgggtgagc
     1921 tggcgctgga agccgatatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa
     1981 ccgcagggga tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta
     2041 cggccaccgg gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg
     2101 gcggtaaatc gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg
     2161 ccctgttcac catcttctcg atcaacggcg aacgctgcac cgccggttcg cgcatcttta
     2221 ttcagcaaag catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc
     2281 gcgtgggcga tccgaacgat ccgaataccc aggttggcgc gcttattagc cagcagcact
     2341 gggaaaaagt ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg
     2401 gcggcccgga taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc
     2461 caaccgtgct ggcagacgtt gataaccgta tgcgagtcgc ccaggaagag attttcgggc
     2521 cggtcgcctg cctgctgccg tttaaagacg aagccgaagg cttacgtctg gcaaacgacg
     2581 tggagtacgg cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg
     2641 cgcgtggcat tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc
     2701 agccatttgg cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg
     2761 aagtgttcgc ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat
     2821 ggggagtctg atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg
     2881 tatcactctg aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat
     2941 aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc
     3001 cactggctgg tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac
     3061 accagcaacg agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg
     3121 gagttggggc agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac
     3181 aacattccca gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa
     3241 gacaagcact tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac
     3301 agccgcaagc tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg
     3361 gtccttgcca gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg
     3421 atgaacagct acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg
     3481 cgcgaaggcc agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac
     3541 ggcgaaggca atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac
     3601 gacggcaagg tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac
     3661 gctgttttcc cgcttcccgc gtaaggacgt tttatgccgc actttatcgt taaatgcagt
     3721 gacaacatac gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg
     3781 gcagccacgg gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc
     3841 tggcagatgg ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc
     3901 gcaggtcgca gcctggaaag ccgccagcag gcgggtgaaa tgctgtttga actgattaaa
     3961 acgcacttcg ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag
     4021 ctgcatccga cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca
     4081 gattgcccgg tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc
     4141 cggataaggc gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca
     4201 cccacaccct gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg
     4261 cgatctcgct ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat
     4321 gggttcgcct gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct
     4381 cgaaagcgat gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg
     4441 acatgttctt ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg
     4501 aagtggagct ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg
     4561 acgtctacaa cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc
     4621 acaacatcga tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg
     4681 ccgccaacgc cggggtgatc ctcggcggtc gtccgatcaa accagacgaa ctggatctgc
     4741 gttggatctc cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg
     4801 gcgtgctgaa tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg
     4861 acgtacaact ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg
     4921 cgcgtaaggg cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct
     4981 ttgtttaagg agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg
     5041 cagatcggat tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca
     5101 ggattcgact ggttgttgat cgacggtgag cacacgccga ataacgtgca aaccgtgctc
     5161 acccagctac aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac
     5221 gatccggtgc aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg
     5281 gtacaaaacg ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt
     5341 attcgcggtg tgggcagtgc gctggcccgc gcctcgcgct ggaatcgtat tcctgattac
     5401 ctgcaaaaag ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg
     5461 aagaacttac cgcagattct ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg
     5521 gatctgagcg ccgatatggg ttatgccggt aatccgcagc acccggaagt acaggccgcc
     5581 attgagcagg cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc
     5641 aatgagcaac tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt
     5701 gacaccaccc tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc
     5761 accgccgtga agcccggcgt gtattaatgc cggatgcgct gcgcttatcc ggcctacact
     5821 cgcaccaaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa
     5881 atcgcggacc ggataaggcg ttacgccgca tccgccataa accttgtcgt accctacaaa
     5941 aatcccatta gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat
     6001 ccggcgaatc agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt
     6061 cgccgcctga gcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac
     6121 atcggctttg ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc
     6181 ctcgctacca ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg
     6241 ctgagtattg tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc
     6301 tctactgcca ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt
     6361 ggcattaccg aagccggctt tctgcctggt attctgctgt atttaacctt ctggtttccg
     6421 gcctatttcc gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg
     6481 ttgggctcga tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc
     6541 tggcagtggc tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg
     6601 ttctggcttg atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc
     6661 ctgcaagaga tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc
     6721 caccacgcca tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg
     6781 tataccctgg cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg
     6841 cagatcctgc aaagttttaa tcagggcagc agtaatatca ccatcggcat gctggccgcc
     6901 gtaccgcaga tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc
     6961 caggaacgaa ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg
     7021 gcttcggcaa ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga
     7081 tcattcagcg caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca
     7141 cgagcgatcg gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg
     7201 tttatgatcg gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt
     7261 gccgcgctgc tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt
     7321 ccgcgagcga ccctgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc
     7381 agcaaagagt acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc
     7441 atggcggcat tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg
     7501 catttcctca atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa
     7561 gcgcccctgt ttgtcctgac gccgccgtca
//
LOCUS       DAZPJP010000195         7477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPJP010000195 DAZPJP010000000
VERSION     DAZPJP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.196"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHU9J_003627"
     CDS             38..250
                     /locus_tag="ACHU9J_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907079.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHU9J_003628"
     CDS             283..501
                     /locus_tag="ACHU9J_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1907080.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHU9J_003629"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHU9J_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHU9J_003630"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHU9J_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1907081.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHU9J_003631"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHU9J_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHU9J_003632"
     CDS             1917..2660
                     /locus_tag="ACHU9J_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1907082.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHU9J_003633"
     CDS             2690..3229
                     /locus_tag="ACHU9J_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1907083.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHU9J_003634"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHU9J_003634"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1907084.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHU9J_003635"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHU9J_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1907085.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHU9J_003636"
     CDS             4582..6102
                     /locus_tag="ACHU9J_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1907086.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..6905)
                     /locus_tag="ACHU9J_003637"
     CDS             complement(6135..6905)
                     /locus_tag="ACHU9J_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1907087.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(7079..>7477)
                     /locus_tag="ACHU9J_003638"
     CDS             complement(7079..>7477)
                     /locus_tag="ACHU9J_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1907088.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT         1980 a         1679 c         1728 g         2090 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct gaacattatc agtgatagtt tgccccgatt gcatagcctg agttgtcacc
     6781 atcatcggtg agtcatagtc catctgttca gtagctgtta aggcaagaaa agattccaca
     6841 ttatctctaa ggtgaaaata gataccatct ttacctgagt atctaatttg ctttgtgtca
     6901 aacattgttg ttattcctat tctgttaagc gtgagctacg tgaaatctaa tttttgatgt
     6961 tggtatctgt tcagaagatt tcacgtagca ttcgactatt catagttgaa tcgtcatact
     7021 agataaagat taatagattg ccatgtgtgg caataaatat tagtagctct aacattgttt
     7081 attcctctgg ataagctgag ctaatcaagg tatcttcctg catacgatta atatgcgatg
     7141 tggttctttc atcaataacc tttccatcaa gtaccgtctg agttgtgata ttgattgtct
     7201 gaggctgaat atttactgac ggtttgataa cctttaaatc ttgtgtgaat gtttccactc
     7261 gacctttcag attatttaat gcttcaggtg aaaactgtgg aacatctttt gatttgccct
     7321 gaaccatagc ccataaaaca gcggctaaag agttcatgtc accagtcaat gcttggtctg
     7381 tgggttccac ctttgacctt acaactttat caattggatt atctgactta ctcatctgac
     7441 gttcatagaa cggtttgaca atggcattaa tcacatc
//
LOCUS       DAZPJP010000196         7417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPJP010000196 DAZPJP010000000
VERSION     DAZPJP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.197"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHU9J_003639"
     CDS             complement(75..1583)
                     /gene="putP"
                     /locus_tag="ACHU9J_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1907089.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2005..5967
                     /gene="putA"
                     /locus_tag="ACHU9J_003640"
     CDS             2005..5967
                     /gene="putA"
                     /locus_tag="ACHU9J_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1907090.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6007..6645)
                     /gene="rutR"
                     /locus_tag="ACHU9J_003641"
     CDS             complement(6007..6645)
                     /gene="rutR"
                     /locus_tag="ACHU9J_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1907091.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6933..>7417
                     /locus_tag="ACHU9J_003642"
     CDS             6933..>7417
                     /locus_tag="ACHU9J_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1907092.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1771 a         2055 c         1943 g         1648 t
ORIGIN      
        1 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
       61 gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
      121 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
      181 actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag
      241 ccgaactgct tccagacgat aactgtcagc gcaccgatga tcatccccgc cagcgcaccg
      301 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
      361 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
      421 gcaatcgcca ccagcgccac caccagcacc atcacacgtc ctacccacac cagctctttc
      481 tggctggcct gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
      541 accagcagct ggcaacttaa ggttgacatt accgccgcca gaatcgccga cagcagaatc
      601 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ttcagcgttc
      661 tggtttaccg cacctgctac cgccggatgc tcgttaaagt aagcgatacc aaagaagcca
      721 acagccaccg cccctgccag gcaaaggatc atccaggtca tactgatacg acgcgcatgg
      781 acaatgctgt ggtgagaatc tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag
      841 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
      901 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
      961 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
     1021 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca
     1081 gctcccgcca acagagccgt ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg
     1141 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
     1201 ataatgcgca aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt
     1261 aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
     1321 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
     1381 accgcgccag gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
     1441 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
     1501 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
     1561 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
     1621 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
     1681 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
     1741 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
     1801 cacctttcaa aaaggttaac tgccgcagag aaaaagtctg agttattttt taatcactgt
     1861 catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc aagtcacatt
     1921 taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt aagttgcacc
     1981 tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac
     2041 gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacacc acactggtta
     2101 attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag
     2161 ctacctgcgc tgctttctgg cgcggccaat gagagcgatg aagcaccgac tccggcagag
     2221 gaaccacacc agccattcct cgactttgcc gagcaaatat tgccccagtc ggtttcccgc
     2281 gccgcgatca ccgcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa
     2341 caagcccgcc tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc
     2401 gataaactgc gtaatcaaaa aaatgccagt ggtcgtgcag gaatggtcca ggggttattg
     2461 caggagtttt cgctgtcatc gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg
     2521 ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc gcgacaaaat cagcaacggt
     2581 aactggcagt cacacattgg tcgtagcccg tcactgtttg ttaatgccgc tacctggggg
     2641 ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg
     2701 aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg
     2761 cgcctgatgg gcgagcagtt cgtcacgggt gaaaccatcg cggaagcgtt agccaatgcc
     2821 cgcaagctgg aagagaaagg tttccgttac tcttacgata tgctgggcga agctgcactg
     2881 actgccgcag acgcacaggc gtatatggtt tcctatcagc aggcgattca cgccattggt
     2941 aaagcgtcta acggtcgtgg catctatgaa ggtccgggca tttcaatcaa actgtcggcg
     3001 ctgcatccgc gctacagccg cgcccagtat gaccgggtaa tggaagagct ttacccgcgt
     3061 ctgaaatcac tcaccctgct ggcgcgtcag tatgatattg gtattaacat cgatgccgaa
     3121 gaggccgatc gtctggagat ctccctcgat ctgctggaaa atctctgttt cgagccggaa
     3181 ctggcaggct ggaacggcat cggttttgtt attcaggctt atcaaaaacg ctgcccgttg
     3241 gtgatcgatt acctggttga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg
     3301 gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc
     3361 tatccggttt atacccgcaa ggtgtatacc gacgtttctt atctcgcctg tgcgaaaaag
     3421 ctgctggcgg tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg
     3481 gcagcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc
     3541 ctgcatggta tgggcgagcc actgtacgag caggtcaccg ggaaagttgc cgacggtaaa
     3601 cttaaccgac cgtgtcgtat ttatgccccg gttggcacac atgaaacgct gctggcgtat
     3661 ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac
     3721 acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg
     3781 caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac
     3841 ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg tctggcctcg
     3901 ctctcctctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa
     3961 caaccggtag cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt
     4021 gtgggctttg tgcgtgaagc cacgccacgt gaagtagaac aggcgctgga aagtgcggtt
     4081 aataacgcgc caatctggtt tgccacgcct ccggctgaac gcgcggcgat tttgcatcgc
     4141 gctgccgtgc taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc
     4201 ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac
     4261 tacgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg
     4321 gtgtgtatca gtccgtggaa cttcccgctg gctattttca ccgggcagat cgctgccgca
     4381 ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg
     4441 caagggatcg ccattttgct ggaagcgggt gtaccccctg gcgtggtgca attgctgcca
     4501 ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg
     4561 tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac
     4621 gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc
     4681 gattcttcag cactgaccga acaagtcgtc gtggatgtgc tggcctcggc gttcgacagt
     4741 gcgggtcagc gttgttcggc actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac
     4801 acgttgaaaa tgctgcgcgg tgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg
     4861 accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt
     4921 cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat
     4981 gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt
     5041 gccgaattgc acaaagaggt ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac
     5101 cagctaccag agctgatcga gcagattaac gcttccggct atggtctgac gcttggtgtc
     5161 catacgcgta ttgatgaaac catcgcccag gtcactggct cggcccatgt tggtaacctg
     5221 tatgttaacc gtaatatggt gggcgcagtg gttggtgtgc agccgttcgg cggcgaaggg
     5281 ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat
     5341 cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat
     5401 gcacagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat
     5461 cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa
     5521 cgattgctgc cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc
     5581 gtgttgtgta ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg
     5641 gtgggcagcc aggtactgtg gccggatgac gcgctgcatc gtcagctagt aaaggcattg
     5701 ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccc
     5761 tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt
     5821 gctgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc
     5881 cttctggaac ggctgtatat cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt
     5941 aacgccagtt taatgacgat cggttaaaca ggtagccgga ggatgttaca acctcctccg
     6001 gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg
     6061 attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa
     6121 atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg
     6181 cgcgagtttg ccgcttttga cccaaccggc aatcagcgca gatttctcat caattaatgc
     6241 cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca tctccataca
     6301 gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc agcttcagac ggatgtactc
     6361 ttttatcgcc gccagcggag cgaaatcttc acgaaacgct tttaacggtg ccagccagat
     6421 atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag
     6481 cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa
     6541 accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac
     6601 tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt
     6661 gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc actctcatcg
     6721 cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt tttatcagat
     6781 attttagata acgcaatatt aacatattaa taacattaac tttttaaaaa ctggcatccg
     6841 ctttgcaaac aagccaatac gcggctgaaa aggatatgca ggatgcagcg ccccgtctga
     6901 ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc gtacctattg
     6961 gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc tttgaactga
     7021 ataaagccat tgtgcagaaa gcggagcatt accatttcga tttcgccctg tcgatgatca
     7081 aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag tcgttcacct
     7141 tgatggcggg tctggcagcc gtgacctcgc gcattcagat ttacgccact gctgccacct
     7201 taacgttacc tccggcaatc gtcgcccgta tggccgcaac catcgactcc atctctggcg
     7261 ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgtgtatgag cagatgggta
     7321 tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgaa tatgttcagg
     7381 tgctgcgcga cctgtggggc acggggaaaa gcgattt
//
LOCUS       DAZPJP010000197         7397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPJP010000197 DAZPJP010000000
VERSION     DAZPJP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.198"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHU9J_003643"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHU9J_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHU9J_003644"
     CDS             complement(4538..4999)
                     /locus_tag="ACHU9J_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR1907093.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6929)
                     /locus_tag="ACHU9J_003645"
                     /pseudo
     CDS             complement(5027..6929)
                     /locus_tag="ACHU9J_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1424 a         2231 c         1980 g         1762 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cctggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatcacaacg agtaacaaaa acgcaaagtt aaaccaatat tcataatata
     7201 tttttattca cagcaataaa taatccaatt aacattatta aaatgaatga ttgcataaga
     7261 aatatatgct accgctccac ccatgataac atgatctgca cattgcacat cttaacgttg
     7321 ttaaatactg gctcaatagt ggatatttta aattaggaat ttatcaaatt cacttgacaa
     7381 atagattgag tgaataa
//
LOCUS       DAZPJP010000198         7392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPJP010000198 DAZPJP010000000
VERSION     DAZPJP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.199"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(39..1298)
                     /gene="rho"
                     /locus_tag="ACHU9J_003646"
     CDS             complement(39..1298)
                     /gene="rho"
                     /locus_tag="ACHU9J_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1907094.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(1383..1484)
                     /gene="rhoL"
                     /locus_tag="ACHU9J_003647"
     CDS             complement(1383..1484)
                     /gene="rhoL"
                     /locus_tag="ACHU9J_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1907095.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(1625..1954)
                     /gene="trxA"
                     /locus_tag="ACHU9J_003648"
     CDS             complement(1625..1954)
                     /gene="trxA"
                     /locus_tag="ACHU9J_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1907096.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            2085..3350
                     /gene="rhlB"
                     /locus_tag="ACHU9J_003649"
     CDS             2085..3350
                     /gene="rhlB"
                     /locus_tag="ACHU9J_003649"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1907097.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            3484..4968
                     /gene="gppA"
                     /locus_tag="ACHU9J_003650"
     CDS             3484..4968
                     /gene="gppA"
                     /locus_tag="ACHU9J_003650"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1907098.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(5015..7036)
                     /gene="rep"
                     /locus_tag="ACHU9J_003651"
     CDS             complement(5015..7036)
                     /gene="rep"
                     /locus_tag="ACHU9J_003651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1907099.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            7253..>7392
                     /locus_tag="ACHU9J_003652"
     CDS             7253..>7392
                     /locus_tag="ACHU9J_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907100.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         1753 a         1892 c         1902 g         1845 t
ORIGIN      
        1 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
       61 aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc cattgcatcg
      121 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
      181 gtagtgagca gttcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
      241 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
      301 tcgtagataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc gataatagtc
      361 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgcagg
      421 gcgttggcat ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa
      481 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
      541 aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
      601 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
      661 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
      721 tgggcaatgt tctgcagcag catggtttta ccggctttcg gcggtgccac aatcagacca
      781 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcagttaa atcttcagta
      841 gaaccgttac cacgttccat acgcagacga gaatttgcgt gcagcggggt taagttctca
      901 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
      961 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
     1021 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
     1081 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
     1141 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
     1201 tcctgcttac gcatgcgagc caggttttcc agccccatat tttcgccgag agtgatcagc
     1261 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
     1321 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
     1381 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
     1441 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
     1501 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
     1561 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
     1621 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
     1681 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
     1741 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
     1801 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
     1861 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
     1921 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
     1981 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
     2041 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
     2101 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
     2161 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
     2221 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
     2281 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcaaccgc
     2341 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gacgcagaac
     2401 cgctggcgga agctactggc ctgaagctgg gtctggctta cggtggtgat ggctacgaca
     2461 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
     2521 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gttctggatg
     2581 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
     2641 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
     2701 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
     2761 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
     2821 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
     2881 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
     2941 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
     3001 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
     3061 cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
     3121 gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa gagtatgcat
     3181 tgaatttgcc tgctattgaa acctatattg gtcactcaat tccggtaagc aaatacaatc
     3241 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca cgtacaggca
     3301 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaatgtc
     3361 atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg atgagcatac
     3421 tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg aaggatgaag
     3481 agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa tagttttcat
     3541 atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa
     3601 gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc
     3661 ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt
     3721 cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt tattgccaat
     3781 gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga agcacgtctg
     3841 atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt ggtggatata
     3901 ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc gttgttcagc
     3961 ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa tctggggcag
     4021 gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa
     4081 ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca ggcgttacag
     4141 gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt gcagcaattg
     4201 aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg gctgacgctg
     4261 gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt taccgaactg
     4321 aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt ctacggcatg
     4381 ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc
     4441 tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa cttcttcgat
     4501 caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat cagcgcctgt
     4561 cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca cgctgcttat
     4621 ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt gctggcgacg
     4681 ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca aaatgccgta
     4741 ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat ttttgccagc
     4801 cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga actgttgacc
     4861 ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat tattgctcag
     4921 gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg agtaagtgcc
     4981 ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt gccgccatca
     5041 tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg gcgctgacca
     5101 ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa aagcggctcg
     5161 gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta aaggtcaatt
     5221 ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg atattatctt
     5281 catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg tagacataag
     5341 gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc agctcttctt
     5401 cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc tgggtgagcg
     5461 tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta aacagttggt
     5521 tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg tacagccagg
     5581 attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc cgctccgcca
     5641 gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa ccacgtccgc
     5701 taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta ttgcgcgtca
     5761 tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc ggcgtgttaa
     5821 cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga taagccagca
     5881 agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg tacgggatgc
     5941 ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga taaagaatag
     6001 cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc aatcagttca ccagtgacgc
     6061 gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg ccgtaaccca
     6121 gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg
     6181 ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc ttcagcgccg
     6241 gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag gagtagatcg
     6301 actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc agtttcacca
     6361 gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag cgaattttgt
     6421 tctgccagcg ctcgcggact tcttcattgc gttgcagcag caacgtcggc aataaaatca
     6481 gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc ccataacaat
     6541 gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac ggtgttttga
     6601 gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact ttgtcatctt
     6661 caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg tcaaacagcg
     6721 aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc agccccagcg
     6781 tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc tgtccgacac
     6841 gctctttcat ctcgcgcgcc gccttattgg taaaggtcac cgccgcgata tgtcgcgcct
     6901 gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga gttttaccgg
     6961 aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct tgttgttggc
     7021 cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga ttgtagcaga
     7081 atcgcatttg aatgtgaccg cgtgtaagcc gctaaccgat tggctgcgga tgtttaaatc
     7141 agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga aaatatcggg
     7201 tcgtaacgtt gtgttattac tttttcagag tttactaagg agaggtgtac tcgtggcgat
     7261 taatttcagt cctaaggttg gtgaaatact ggattgcaac tttggtaact atcctgttag
     7321 ccagaatgga cagttcagca ccacgtatta cgatggccgc attcctcctg aaatgataaa
     7381 aaacaggtta gt
//
LOCUS       DAZPJP010000199         7343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPJP010000199 DAZPJP010000000
VERSION     DAZPJP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.200"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            166..1230
                     /gene="ulaG"
                     /locus_tag="ACHU9J_003653"
     CDS             166..1230
                     /gene="ulaG"
                     /locus_tag="ACHU9J_003653"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1907101.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1338..2093
                     /gene="ulaR"
                     /locus_tag="ACHU9J_003654"
     CDS             1338..2093
                     /gene="ulaR"
                     /locus_tag="ACHU9J_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1907102.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2090..2839)
                     /gene="yjfP"
                     /locus_tag="ACHU9J_003655"
     CDS             complement(2090..2839)
                     /gene="yjfP"
                     /locus_tag="ACHU9J_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1907103.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3021..3350
                     /gene="bsmA"
                     /locus_tag="ACHU9J_003656"
     CDS             3021..3350
                     /gene="bsmA"
                     /locus_tag="ACHU9J_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1907104.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3499..3774
                     /gene="yjfN"
                     /locus_tag="ACHU9J_003657"
     CDS             3499..3774
                     /gene="yjfN"
                     /locus_tag="ACHU9J_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1907105.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3891..5516)
                     /locus_tag="ACHU9J_003658"
     CDS             complement(3891..5516)
                     /locus_tag="ACHU9J_003658"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1907106.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5600..6763)
                     /locus_tag="ACHU9J_003659"
     CDS             complement(5600..6763)
                     /locus_tag="ACHU9J_003659"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1907107.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6766..>7343)
                     /locus_tag="ACHU9J_003660"
     CDS             complement(6766..>7343)
                     /locus_tag="ACHU9J_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1907108.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1919 a         1962 c         1797 g         1665 t
ORIGIN      
        1 atttaactac acattccatt aaatctttcc acactcaaaa tcaacaacaa cttccctggc
       61 aaaaagtcaa aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc
      121 aatcacatat tgattcgaat tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa
      181 agtatcaccc gagaatcctg gatcctgagc actttcccgg agtggggtag ctggctgaat
      241 gaagaaattg aacaagagca ggtcgcccct ggcacatttg cgatgtggtg gctaggctgc
      301 accgggatct ggttgaaatc agaaggtggc accaacgttt gcgttgattt ctggtgcggc
      361 actggcaaac aaagtcacgg taacccgtta atgaaacagg gtcaccagat gcagcgcatg
      421 gctggcgtga aaaaactgca gccaaaccta cgcaccaccc cgtttgttct tgatccgttt
      481 gcgattcgcc agatcgacgc ggtactggcg actcacgatc acaacgatca tatcgacgtt
      541 aacgtcgctg ctgccgtgat gcagaactgt gctgatgacg taccattcat tggaccgaaa
      601 acctgcgttg atttgtggat tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa
      661 ccgggcgatg ttgtaaaagt gaaagacatt gaaattcatg cgcttgacgc tttcgaccgt
      721 actgcactga tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc agatggcatg
      781 gacgatcgcg cagtaaacta cctgttcaaa acccctggcg gttccctata tcacagtggc
      841 gattcccact actctaacta ttatgcgaag cacggtaacg aacatcagat cgatgtggcg
      901 ttaggatcgt atggtgagaa cccgcgcggt attaccgaca aaatgaccag cgccgatatg
      961 ctgcgtatgg gtgaagccct gaatgcgaaa gtggttatcc cgttccacca cgacatctgg
     1021 tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgt
     1081 ctgaagtatg gcttcaagcc gtttatctgg caggttggcg gcaaatttac ctggccgctg
     1141 gataaagaca acttcgagta ccactatccg cgcggtttcg atgattgctt cactattgaa
     1201 ccggatctgc cgttcaagtc attcctgtaa tctgtcgttt ataccggata gcgtggagaa
     1261 gttcgcgcta tccggcgtag tatttcctag ctatttcaaa tatcatctaa aaaaatcaaa
     1321 ttttatcgga atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg
     1381 cacaattggg ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca
     1441 ctgcgcgacg cgatatcaat aaacttgacg aacgcggcaa actgaaaaag gtgcgcaatg
     1501 gcgcagaagc tattacccaa cagcgcccgc gctggacgcc gatgaatctg catcaggcgc
     1561 agaatcacga tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg
     1621 aaagcgtagt catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca
     1681 agccagtgca aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaagaac
     1741 atgacagcgt gatcattatg ggtggacagt acaacaaaag tcagtccatc actttaagcc
     1801 cgcagggcag cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggaaaagggc
     1861 tgaccgcaga agggttgtat aaaaccgata tgctgacagc aatggcagag cagaagatgc
     1921 tgagcgtggt agggaaactg gtggtactgg ttgatagcag taagattggc gaacgcgcgg
     1981 gaatgctttt tagccgtgcc gatcaaatcg atatgcttat caccggcaaa aatgctaacc
     2041 cggaaatcct gcaacaactg gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct
     2101 ggcggaaaaa tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg
     2161 gctgccatga acaggttagc agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac
     2221 gtagtgattc gtcggcaggc acaacgtcat cgtcgaggcc atgccacagc agtagaggtc
     2281 tgtccccaag ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgataatgt
     2341 tattgaattc attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg
     2401 cgagcgatgt aaaatagccc gatcccatca tgctggcggt acatttcacc gtggggtggc
     2461 gagcggtaat tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt
     2521 catcaagcag ccagttttct tcggctattg ccgcacgtaa agtagtgaat tcctgcatac
     2581 tttgtagcaa gatttgccag aattgattta accgccgcgc ttcgtcgcca ctaaaacggc
     2641 taccgtgatc gggcgcatcc ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg
     2701 caaaatagct atacaccagg ctggatgaag taaagccgtg ataaaaaatt acgcacggta
     2761 acggggtatc tttttgcccg acaggataag catgaagaac gggaatatct gccagctcgc
     2821 gtgattctat ttcaatcatc tgtctctcct ttttgtctgg tatgacatga agaaatcctt
     2881 agcacaagtt tctgctggcg gtattagttc ataatgttga gatgtgggtt acgctttcgt
     2941 cacgttttca ttcgaaaatc gcgtaccagg taacaattcg ggaacattcc cccaaagtta
     3001 aattaataag gcactacact atggttagca ggaaacgtaa tagcgttatt taccggtttg
     3061 ccagtttatt attggtgttg atgttaagtg cctgtagcgc actgcaaggt acgccacagc
     3121 cagcaccgcc agtaacggac catccgcaag agattcgccg cgaccagacg caagggttac
     3181 agcgaatagg tagcgtaagc acgatggttc ggggttctcc ggatgacgca ttagcagaaa
     3241 tcagagcaaa agctgtcgct gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa
     3301 ccatcgtgac aggacagtgg tattcacaag ccattttgta ccgtaaataa atcagacaac
     3361 ttacatagtc agctttacat tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt
     3421 accggctgga agcctgggcg tttattaccg cgatggaatg ccctgcaaca tgtggggaaa
     3481 cgaaaaatgg agctgacgat gaaacaatta cttgcctcac cctcgctgca attagtgact
     3541 tatcctgcga gcgccacggc acagtctgca gaattcgcca gtgctgactg cgtgacgggc
     3601 ctgaatgaaa taggtcagat ctcagttagc aatatttcag gggatccgca ggacgtggaa
     3661 cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg
     3721 tacgaagacc agcagcctga taactggcgc gtacaggcga tactgtatgc atgagtgtca
     3781 tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc ttgtccggcc tacaattttc
     3841 acatatttca ttgagttaat cgaaaccagc gtcgcatcag tcgtatacgt ttacaaacac
     3901 actccccccg tcgcccgcag caataaatca ttctggatct gctctgacaa ccgtacgccg
     3961 ccgcgcgtat ctaacatcac ctgacaccac gcctgcgcca ttggcggaga agcatatttc
     4021 aacatttgcg caccgcagcc cagcaggaat agctgatgag taatctctcg ccccagttct
     4081 tcagctggct tacgcagctg ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc
     4141 tgccctttca cttccacaaa tgcttccgac aataagtcgt atacgcccgc ttgcttattg
     4201 agaacgcgca acacatccag gcacataata ttgccggaac cttcccaaat actgtttacc
     4261 ggcatctccc ggtaaagccg cggcagctcg ctctcctcgc aataaccaat gccgcccagc
     4321 acctccatcg cttcggccac aaacggcata cctcgtttgc agatcacaaa ttttgccgca
     4381 ggcgtaaata aacgcgccca cagggcttct ttggcatcgg cgcgccggtc ccacgctcgc
     4441 gcaagacgaa acagcaacgc cgtttgccct tcaagctgaa gtgccatgcg acttaagaca
     4501 tgacgcataa ggggctgttg gatcaatgga ttaccaaaaa catggcgttg atgtgcatga
     4561 taaatcgcca gcgaaaacgc acggcgcatc atggcatggc tacccagggc gcaatcaaaa
     4621 cgcgtcatcc cccccatttt caggatcaga cgaattcctt ccccttcctg ccccaataac
     4681 cagccaatgg catcctgaaa ctccacttca caactggcgt tagagcgatt acccagctta
     4741 tctttcagcc gttcgaggcg aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca
     4801 aaaaaacagg acagaccgcc cgtggtctgc gccagcacca gatgcgcatc gctttgcgga
     4861 accgagaaaa accatttatg ccccaccagc cgataagagc catcttccaa acgctctgca
     4921 cgggtggtgt tgctcatgac atcggaacca ccctgctttt ccgtcattcc catgccaatc
     4981 aacaaaccgc gtttttgccc acctggcaat aagtgagaat cgtagcgatc gctcagcagc
     5041 ggcgtggtcc agtcctgaaa cggcgtgggt aacatctgta acaacaatgg cgtggcggca
     5101 aaggtcatcg ttattggaca taacgaccca gcctcaacct gcgcatgtaa cataaaacgc
     5161 gccgcgcgcg ccacaaatgc gccggagcga gcgtcttctt cccaggcaag attgtgcacc
     5221 cgattggtac ataacacctg catcagcagg tgccatgcgg gatggaaacg aacatcgtcc
     5281 agacgccgcc cctgggcatc gtagcgcaac agttcaggcg ggtttacatt cgccagccgc
     5341 cccagttcaa gggattcagc cgttcctaac tgctgaccaa tactggctag aaaatcgcta
     5401 tcccagccag caccttcacg cgttaccgct tcgcaaagcg cgccatcaga caggtataag
     5461 ttgctgttat ttaatggtat aggttgatta aaaacggtgt gagtttgcca gtgcactgtg
     5521 tctccctcca tcaatggcag tcaccattag tatggccact gccatggatt catgacagaa
     5581 agagattgct aaaacattct tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt
     5641 tggtgatcag tgtgttatct tcacggatcc ccattccgca cgcttcatca tcgacaatcc
     5701 agctatcgat gagtgtgtag ctatcgccaa atcgcggcag aggttgaaac gcctggtaga
     5761 tcatcggttc atcggcgtaa tcaccatcag cgtggtcaac gacgttattc tgaccgtcga
     5821 aaatggtgac gtttccgcct tcgcgcgagt agatcggttt acgcacatag ctttcgccag
     5881 cggcaatctg cgatttttca ccctcaaacc aggatgcaag aaggttagga tgaccaggga
     5941 agaagcgcca aagcagcggc attagccctt tattactcaa gatacttttc cataacggct
     6001 ccacccattg ctcgcgacgc ttgcacagca gcggaccgtt atcgtcacgc atcatccact
     6061 ccagcggata cagcttaaat gcacgctgga tgacattatc atcaagatcg gtcagtacac
     6121 cgccgacgcc caaaccgaga tcttcaatgt agataaaccg cgactcctgc cctgcctgct
     6181 gggcgcagtc ctgcaagtac agcacggtag tcctgtcttc atcggtgtcc tgacagcagc
     6241 agaaataaaa cggctcccgg ctataaagct cgctaaagcg tgaaatcagg cgttcctgaa
     6301 tggcattgta ctgatcggca tcacgcggaa taatgccgct gcgccgggca tcctccagcc
     6361 acaaccactg gaaataagcc gactcataca acgaagtcgg cgtatcggcg ttgtactcca
     6421 gcagcttcac cggcgcattg ccacaccagg caaaatccat ccggccatac agcgaaggat
     6481 cgctggcgtg ccagctttcg gcgatcacat cccagtacaa cggcgggatc gccagttgcg
     6541 tcaggatctc ttcgtcttta accgcgcgat ccaccacctc aaggcacatc tgatgcagtt
     6601 ccgcagttgg tttttcgatc tgctcttcaa tctggcgcag agtaaaacgg taagcccgac
     6661 tctcgtccca atagatttca ttgtcgatga tatgaaagtc gaaaccgttg tcagcggcaa
     6721 tttggtccag atcccgccgc acaggaacgt tgtgtctcag catgattagc ctccccaatg
     6781 cccacgggcg ctggaagaac gaccatagcc gccgcgcgaa acggttgacg cttttttggt
     6841 ggtaaagccg cctgaagagt aagactcttt tttgccagaa ccggagcgcc aggagtaatc
     6901 accggaagtg ttgcgccaga cggggcggga agcaaatgag gatccgccgc tgttatagac
     6961 gaacggctca tcgcgatctt tacgaataac ccggcttaac aaaaatccag aaacgaccgg
     7021 gatccaactc tgttcaacat tgtcgtagta gcaattttcg tacttagact gacagttctg
     7081 ctgagtcata ttcttcggaa catcggcata aaatgccgtt ttggcgttgt tccagccacg
     7141 cgcgcaaatg tcggcattat taccgtcatc aatacaatcc tgcactgtcg cgtaaaacgt
     7201 tccgtcgccg tcgttatcga catcgctact atcgccacaa cctttcaata cgaaaaaagc
     7261 ggcaccgccc attagcgcca acgttaagta tttttgtgcg tagcgattgc gacgcggttc
     7321 aaaaggatta ttcggtctac caa
//
LOCUS       DAZPJP010000200         7333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPJP010000200 DAZPJP010000000
VERSION     DAZPJP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.201"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            2..262
                     /gene="dinJ"
                     /locus_tag="ACHU9J_003661"
     CDS             2..262
                     /gene="dinJ"
                     /locus_tag="ACHU9J_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR1907109.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            265..543
                     /gene="yafQ"
                     /locus_tag="ACHU9J_003662"
     CDS             265..543
                     /gene="yafQ"
                     /locus_tag="ACHU9J_003662"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR1907110.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            699..1439
                     /gene="dpaA"
                     /locus_tag="ACHU9J_003663"
     CDS             699..1439
                     /gene="dpaA"
                     /locus_tag="ACHU9J_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1907111.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1410..2177)
                     /locus_tag="ACHU9J_003664"
     CDS             complement(1410..2177)
                     /locus_tag="ACHU9J_003664"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1907112.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2383..2961)
                     /gene="lpcA"
                     /locus_tag="ACHU9J_003665"
     CDS             complement(2383..2961)
                     /gene="lpcA"
                     /locus_tag="ACHU9J_003665"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1907113.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3201..5645
                     /gene="fadE"
                     /locus_tag="ACHU9J_003666"
     CDS             3201..5645
                     /gene="fadE"
                     /locus_tag="ACHU9J_003666"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1907114.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5688..6134)
                     /gene="ivy"
                     /locus_tag="ACHU9J_003667"
     CDS             complement(5688..6134)
                     /gene="ivy"
                     /locus_tag="ACHU9J_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1907115.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6315..7085
                     /gene="yafV"
                     /locus_tag="ACHU9J_003668"
     CDS             6315..7085
                     /gene="yafV"
                     /locus_tag="ACHU9J_003668"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1907116.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(7127..>7333)
                     /locus_tag="ACHU9J_003669"
     CDS             complement(7127..>7333)
                     /locus_tag="ACHU9J_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1907117.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1732 a         1852 c         1956 g         1793 t
ORIGIN      
        1 aatggctgct aacgcgtttg ttcgcgcccg aatcgatgaa gatctgaaga atcaggcggc
       61 ggacgtactg gccgggatgg ggctgaccat ctctgacctg gttcgcataa ccctcacaaa
      121 ggtcgcgcgt gaaaaggcat tgccgtttga tttacgcgag cctaatcaat taaccattca
      181 atcaatcaaa aacagcgaag ctggcgttga tgttcataag gccaaagacg ctgatgattt
      241 atttgataaa ttaggaattt aaatatgatt caaagggata ttgaatactc gggacaattt
      301 tcaaaggatg taaaacttgc acaaaagcgt cataaggata tgaataaatt gaaatatctt
      361 atgacgcttc ttatcaataa tgctttaccg cttccagctg tttataaaga ccacccgctg
      421 caaggttcat ggaaaggtta tcgcgatgct catgtcgaac cggactggat cctgatttac
      481 aaacttaccg ataaactttt acgatttgag agaactggaa ctcacgcggc gctctttggg
      541 taaagcacgc tgccgatgaa atttgtaaac cctgcgccga caggcttgcg taaaactctt
      601 ttggtgataa tgtgagcctt ccaaatccag actattccga tttgggatct attttgccgt
      661 cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc gcattaattc
      721 ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc agtagctcga
      781 ccacgccagt gagcaaagag tataagcagc agttgatggg atcccctgtc tacatccaga
      841 tcttcaagga agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc
      901 tcgacagtta taaaatctgt aaatattccg gcggcttagg gccaaaacag cgtcagggcg
      961 atttcaaaag cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc
     1021 gttactacaa agcgattaat attggtttcc ccaatgccta tgaccgtgcg catggttatg
     1081 aagggaaata cctgatgatc cacggcgatt gtgtttccat cggctgctac gcaatgacca
     1141 atcagggtat tgatgagatc ttccagttcg ttactggtgc gctggtgttt ggtcagccga
     1201 gcgtgcaagt gagtatttac ccgttccgca tgaccgacgc caatatgaag cgccataaat
     1261 attccaactt taaggacttc tgggagcaac tgaagccggg ttacgactac tttgagcaaa
     1321 cccgtaagcc accgaccgtt tctgtggtca atggtcgtta cgtggtcagc aagccgttaa
     1381 gccacgaagt tgtgcagcca caactggcat caaactacac gctccccgag gcaaaataag
     1441 cgccattcgc ctggcataat cttttgccag gtttcattgc ccgtcagcgg ctgtgttgca
     1501 atcaccgtga ccacatcatt cggtgtggtc tgcgagctga agtcgatttc cacatcctga
     1561 tccagcagcg ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa attagtcgag
     1621 caatacgcca tcacatagcg cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc
     1681 agttcatccg ccagtgaggc gatatattta aacactgccg ccatgttgcc cggtgtgcgc
     1741 gggtaacgct gcgttaattt atgcaggagc cagcagaagg ctttttcgct atcggtttca
     1801 ccgaccgggc ggaagttgcc ggtttccagt gatttgtagc ccgtcagttg tccgttatgg
     1861 gcataagtcc agttacgccc ccataactca cgggtaaacg ggtgagtatt ttccagcgcc
     1921 acctcgcccc gattggcctg gcgaatatga gccaccaccg aacaggattt tatcgggtag
     1981 tcctggacaa gtttggcgat gggggaatta aagctgggtt gtggatcttt aaatgtgcga
     2041 cagcctttac cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc ggttccacca
     2101 ccacgctgca ccagaccggt gaaactaaag cagatatcgg tagggacgtt ggcgctcatc
     2161 ccgagcaatt cgcacatggg taacctccac aacagcggga gcgcatggcc cccggcgaag
     2221 attgatttaa gaattttcag gccggatgcg gcgtaaacgt cttattcggc ctacggttcg
     2281 cgtacagatt tgtaggcatg ataagacgca tcagcgtcgc atcaggcatc agcgcacaag
     2341 tgccggatgc ggcgtaaacg ccttatccgg cctacgccag acttacttaa ccatctcttt
     2401 ttcaattaac tggatcagga tatggatcac tttaatgtga atctcctgaa tgcggtcggc
     2461 ataaccaaag tgcggaacgc gaatttcgat atccgccgtg ccagccattt taccgccgtc
     2521 tttaccggtc agggtgatca ctttcatccc cttctcacgc gccgctgcga tcgctttgat
     2581 cacgtttgca gagttaccgg aggtggagat ccccagcagt acatcgcctt cgcgacctac
     2641 cgcttcaacg tagcgggaga aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat
     2701 atgactaacg tcagaaatag caatcgccgg gtagcccgga cggttttcac ggtagcgacc
     2761 ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag
     2821 cactttgcca ccggctttaa agctgtctgc taacaggacc gccgcgcgct gaatggcgtg
     2881 aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt
     2941 acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag acaaaatgca
     3001 gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca ggactttttg
     3061 acctgaagtg cggataaaaa cagcaacaat gtgagctttg ttgtaattat attgtaaaca
     3121 cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta
     3181 caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacggtt gtcctgctcg
     3241 gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg ctcgcctgga
     3301 cagccgccct cggcgttgct ggtctgtggt cagcgtgggt actggtgcct ctggccatta
     3361 tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc
     3421 gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg
     3481 gcaccacctg gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata
     3541 actatccgca gccgcgcctg accgccgaag agcaagcgtt tctcgacggc ccggtagaag
     3601 aagcctgccg gatggcgaat gatttccaga ttactcatga gctggcggat ctgccgccgg
     3661 agttgtgggc gtaccttaaa gagcatcgct tcttcgcgat gattatcaaa aaagagtacg
     3721 gcggactgga gttctcagct tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga
     3781 gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggcccgggc gagctgctgc
     3841 aacattacgg cactgacgag cagaaagatc actatctgcc gcgtctggcg cgtggtcagg
     3901 agatcccttg ctttgcactg acaagcccgg aagcgggttc cgatgcgggc gcgattccgg
     3961 acaccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg atgcgtctga
     4021 cctggaacaa acgctatatt acgctggcac cgattgcgac tgtgcttggg ctggcgttta
     4081 aactctccga cccagaaaag ctgctgggtg gcgcagaaga tttaggtatt acctgtgcgc
     4141 tgatcccgac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac
     4201 cgttccagaa cggaccgacg cgcggtaaag atgtcttcgt accgatcgat tacatcatcg
     4261 gtgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc
     4321 gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcggtagcg ctggcaacgg
     4381 gcgcgtatgc tcacattcgc cgtcagttca aaatctctat cggtaagatg gaagggattg
     4441 aagagccgct ggcgcgtatt gccggtaatg cctacgtgat ggatgctgcg gcatcgctga
     4501 ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc
     4561 actgtaccca ccgcgggcag cagtcgatta ttgatgcgat ggatatcact ggtggcaaag
     4621 gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcgca ccgattgcca
     4681 tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga caaggagcga
     4741 ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat gacgtcaacg
     4801 cgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct
     4861 tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat gccactaaac
     4921 gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct gatgtctcga
     4981 tggcagtgct ggacggcagc ctgaaacgtc gcgagcgcat ctcggcccgt ctgggggata
     5041 ttttaagcca gctctacctc gcctctgccg tgctgaagcg ttatgacgac gaaggccgta
     5101 atgaagccga cctgccgctg gtgcactggg gcgtacaaga tgcgctgtat caggctgaac
     5161 aggcgatgga tgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg
     5221 tggtgatctt cccgaccgga cgtcattatc tggcaccttc tgacaagctg gatcataaag
     5281 tggcgaagat tttacaagtg ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc
     5341 tgacgccgag cgagcataat ccggttggct tgctggaaga ggcgctggtg gatgtgattg
     5401 ccgccgaccc aattcatcag cggatctgta aagagctggg taaaaacctg ccgtttaccc
     5461 gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa gatgaagccg
     5521 ctattctggt gaaagctgaa gaaagccgtc tgcgcagtat taacgttgat gactttgatc
     5581 cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg
     5641 cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta
     5701 aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac cgttttacca
     5761 tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt tctcttgcga cgttttctca
     5821 tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata
     5881 cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt atcatctccc
     5941 aacgttacgg tttgtgcggg agtataagta ccgcctttca tcacccaggc aggcagctta
     6001 tgcccttgta ccatctgatt aaatgcagct ttggtggttt cgccctttgc aaggctgcta
     6061 atggttaaat catcctgcgc cattgcactg gtggcgatga ccagagcggc gactgtcgtt
     6121 attgtcttaa acatcattcc tcccgagctt atcctgccca tgttattaac cttctaaagc
     6181 ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt tgttatcaat
     6241 ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc cttcgcataa
     6301 aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca ctggtgtgga
     6361 tggatggccc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt accgggcgcg
     6421 atgtgatcgt tctaccgaag atgtttacca gcggctttgc catggaagcg gcagcttcgt
     6481 cgctagcaca agatgacgta gtggactgga tgacagctaa ggcgcagcag tgcaatgcgc
     6541 tgattgcagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc tttttgctgg
     6601 ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc atggcagatg
     6661 agcatctaca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta
     6721 ttttgccgtt ggtgtgctac gacttacgtt ttccggtgtg gtcgcgcaat ctcaacgatt
     6781 atgacctcgc catctacgtt gccaactggc ctgctccgcg ctctctgcac tggcaggcat
     6841 tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca
     6901 gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaatcca caaggcgaga
     6961 ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgcggagctg tcgatgatgg
     7021 cattgcggga atatagagaa aagtttccgg catggcagga tgcggatgag tttaggttgc
     7081 ggtgagtggg ggttgtgttt aaggctgggg gagagtcggg gtaagactac gaaagcccgc
     7141 tctccgcaag gactgacgcc agatagtttc tgtccatagc tgcttttcgc atcttacgtc
     7201 ttaaccctgc cttgaatacc ttatcattcg tcaaaatatt aatagcgatg tgccgtatcc
     7261 ctgaaaataa ttctgctgca tttcctcttc ttattttgca gtcgtcttca ttcattacca
     7321 cgtccagacg cca
//
LOCUS       DAZPJP010000201         7301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPJP010000201 DAZPJP010000000
VERSION     DAZPJP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.202"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..71)
                     /locus_tag="ACHU9J_003670"
     CDS             complement(<1..71)
                     /locus_tag="ACHU9J_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907118.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            690..1460
                     /locus_tag="ACHU9J_003671"
     CDS             690..1460
                     /locus_tag="ACHU9J_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1907119.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1451..1924
                     /locus_tag="ACHU9J_003672"
     CDS             1451..1924
                     /locus_tag="ACHU9J_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1907120.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2032..2571
                     /gene="dnaT"
                     /locus_tag="ACHU9J_003673"
     CDS             2032..2571
                     /gene="dnaT"
                     /locus_tag="ACHU9J_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1907121.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2574..3311
                     /gene="dnaC"
                     /locus_tag="ACHU9J_003674"
     CDS             2574..3311
                     /gene="dnaC"
                     /locus_tag="ACHU9J_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1907122.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3360..3854
                     /gene="yjjA"
                     /locus_tag="ACHU9J_003675"
     CDS             3360..3854
                     /gene="yjjA"
                     /locus_tag="ACHU9J_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1907123.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4108..6399
                     /gene="opgB"
                     /locus_tag="ACHU9J_003676"
     CDS             4108..6399
                     /gene="opgB"
                     /locus_tag="ACHU9J_003676"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1907124.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6538..>7301)
                     /gene="lgoD"
                     /locus_tag="ACHU9J_003677"
     CDS             complement(6538..>7301)
                     /gene="lgoD"
                     /locus_tag="ACHU9J_003677"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1907125.1"
                     /translation="PYVACQQCPACKSGRTNCCEKISVIGVHQDGGFSEYLSVPVANI
                     LPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAIAKADGAQV
                     VVADTSPARREHVATRLELPLLDPSAEDFDAQLRAQFGGSLAQKVIDATGNQHAMNNT
                     VNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRLMAEGKIT
                     ADMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         1845 a         1895 c         1793 g         1768 t
ORIGIN      
        1 tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt tttgcagcat
       61 cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt attcaggata
      121 ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta tccttagcat
      181 tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg tcagaaggtt
      241 agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct acattaagtt
      301 gttaggaata cttaatgaaa aacagaaatc acattcattt aatttataat taaatgatat
      361 cccttcccgc agaatgaaaa atcacacaac caatatttat tcaatgaaat ctgatggttt
      421 taacttgtta acattaaaaa acgtgcccgg ttatctatta tcctgattcg taaaggccaa
      481 acaataaatt cattatagtg ataatattca tgtgatagtt tgtttgcggc gagagataat
      541 tcgcttttta tcaccgagct tgtcccgata accaggatta atcagcgcaa ttttgatctt
      601 aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat ctgaacaata
      661 cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga gccgtaacac
      721 ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc gcgttggttg
      781 atgagctttc ctcacgactg ggtcgggcgt tggggatgga cagcgtcgaa agttctatct
      841 cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca tcgacacgta
      901 aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac attgtgattc
      961 tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc caaattcagc
     1021 cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgc gcctgtttct
     1081 gcaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc agtacggccg
     1141 cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccgcagatc aacttttgcc
     1201 tcaccgcttt cgccgccacc accatttccg gattgctttt gcaacttccc actttcagca
     1261 atacacccac tatcgcgatg gccgccagcg ttctgctgct ggtgccggga tttccgttga
     1321 ttaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca cgctgggcga
     1381 tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg acgatttggg
     1441 ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata tgatcctcgc
     1501 cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg cattacgctg
     1561 gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga ccagcgggct
     1621 gaatattgag tggtcaacct ttatggcctc catgctggtc ggcaccattg gtattcaatg
     1681 gtcgcgctgg tatctggcgc atccgaaagt gtttaccgtg gcggccgtta tccctatgtt
     1741 cccgggcata tcggcttata ccgcaatgat ttcggcggta aaaatcagcc agttaggtta
     1801 cagcgaaccg ttgatgatta ccctgttaac caactttctt acagcttcat cgattgttgg
     1861 tgcgttatcc atcggtcttt ccattcctgg attatggttg taccgcaagc gccctcgcgt
     1921 ataaaattgc ctcgcttaaa gcacacggat gagagacagc ctcctctcct ccgtgtgtta
     1981 ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag tatgtcttcc
     2041 agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca ccaaaccgtt
     2101 ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatgcccc ggcgttttat
     2161 gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct ggcgcgtccg
     2221 ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc tcccgttgct
     2281 gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga ttttatccgc
     2341 ctggcggcgc tatggggtgt ggcactaaga gagccggtga ccaccgaaga actggcctca
     2401 ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg gcaacaaaaa
     2461 ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa acgagatgtg
     2521 aatacggtca gcgaacctga cagtcaaatt ccaccaggat tcagagggta acgatgaaaa
     2581 acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc aaacctgcat
     2641 tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc cgctccgccg
     2701 ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc tccggtattc
     2761 ggccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa gggcagatga
     2821 atgcgttaag caaagcgcgc cagtacgtcg aagagtttga cggcaacatc gccagcttca
     2881 tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc tgcaacgagc
     2941 tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg tcggcgatga
     3001 aagatacctt caggaatagc ggtaccagtg aagaacaact gcttaacgat ctgagcaacg
     3061 ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac gaaaaagtga
     3121 tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaaccggg atgctgacca
     3181 acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac cgtatgcgcc
     3241 tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgg gtaacaggta
     3301 aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct tgagtaatga
     3361 tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca ttaatttcca
     3421 ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc gaacttggca
     3481 accaaaacag cacgacacag gaaggcggtt ggtcgctcgc gtcattaact aacttgctta
     3541 gcagcgggaa ccaggcctta agcgcagata acatgaacaa cgccgcaggc attctgcaat
     3601 actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga aaacatcaag aaccaggtgc
     3661 tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat ctggatggca
     3721 ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac atcggaacga
     3781 ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag caggggctga
     3841 acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg acgcggctct
     3901 ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga agcgaccgcg
     3961 atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt gacactagaa
     4021 ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa cgttggctaa
     4081 acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc cctttttctc
     4141 gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt tgcagccaca
     4201 ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag cgactatttt
     4261 actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct gaccggtgct
     4321 ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac agcggtgttc
     4381 ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt tggttacagc
     4441 ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt tcgtcagata
     4501 acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc ttattataaa
     4561 gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta cggcgaaagt
     4621 ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga actgggcgcg
     4681 ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac ggattacacg
     4741 attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt tgaaggcaac
     4801 gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga tatcctgaaa
     4861 aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc cggtaaagat
     4921 gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct gaaaagcgtg
     4981 gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt tctcgatgaa
     5041 gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact gtttaccctg
     5101 acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg caaaaaatat
     5161 gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca ggagaatatc
     5221 gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt catcgtcgtc
     5281 tcttctgacc atttagcgat gaacaacacg gcgtggaaat acctcaataa gcaggatcgc
     5341 aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct ggcagtgaag
     5401 cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga taactatctc
     5461 ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct caatatcaaa
     5521 gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc taaagagatg
     5581 aaagagttca ccatcgacca gcagaaaaac atgattgcct tctcgggtag ccatttccgt
     5641 ttgccgctgc tgttgcgggt ttcagacaaa cgcgtggaac cgctgccgga aagcgaatac
     5701 tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt cgtctgggtt
     5761 gaccgttgct ataagatggc acaactctgg gctccggaac tggcactctc caccgactgg
     5821 tgtgtctcgc aagggcagct tggcggtcag caaattgttc agcatgttga caaaacaaca
     5881 tggcagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta taaaggcaat
     5941 gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag tttcatcttc
     6001 aacgtcgccg gtgcgccaga agaggtgaaa cagtttagtg ggatttcccg tccagagtcg
     6061 tggggccgct ggtccaacgc gcagctgggc gatgaagtaa aaatcgagta caagcatccg
     6121 ctgccaaaga aatttgacct ggtgattacc gccaaagcat acggcaataa cgccagccgt
     6181 cctattccgg tacgcgtagg caatgaagaa caaacccttg tgctgggcaa tgaagtgacc
     6241 accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat tgtgccgccg
     6301 gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa gctcgggatc
     6361 ggcatggtgg aaattaaagt ggtggaacgt gaaggataat tcctgactga tgtgtaggtc
     6421 gcataaggtg cgacctacac accttctgcg gcctacaaaa catcattgag aaacagaaaa
     6481 aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct cgccttatca
     6541 gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct cgtaggtttc
     6601 tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcagcagtga tttttccttc
     6661 cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg gcgttgcggc tacccatcat
     6721 cgtcgtttct tttttatgga attccggatc ggagaactgc aactcacctt taaacaggcc
     6781 gacaaatacc accgtgccgc cgtgacgaat caaattcacg gtgttattca tcgcatgttg
     6841 attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg cccgtagctg
     6901 cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat tccagacgcg ttgccacatg
     6961 ttcacggcgc gccggactgg tatccgccac caccacctgt gcgccatcgg ctttagcgat
     7021 tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct cgccgggagc
     7081 aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca atgctgccgc
     7141 ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca aatactcact
     7201 aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat tggtacgccc
     7261 gcttttacac gccgggcatt gctgacaggc aacataaggg a
//
LOCUS       DAZPJP010000202         7193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPJP010000202 DAZPJP010000000
VERSION     DAZPJP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.203"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            357..1394
                     /gene="dusA"
                     /locus_tag="ACHU9J_003678"
     CDS             357..1394
                     /gene="dusA"
                     /locus_tag="ACHU9J_003678"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1907126.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1528..1770
                     /gene="pspG"
                     /locus_tag="ACHU9J_003679"
     CDS             1528..1770
                     /gene="pspG"
                     /locus_tag="ACHU9J_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1907127.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1936..2919)
                     /locus_tag="ACHU9J_003680"
     CDS             complement(1936..2919)
                     /locus_tag="ACHU9J_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1907128.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3002..4417
                     /gene="dnaB"
                     /locus_tag="ACHU9J_003681"
     CDS             3002..4417
                     /gene="dnaB"
                     /locus_tag="ACHU9J_003681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1907129.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4470..5549
                     /gene="alr"
                     /locus_tag="ACHU9J_003682"
     CDS             4470..5549
                     /gene="alr"
                     /locus_tag="ACHU9J_003682"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1907130.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5802..6995
                     /gene="tyrB"
                     /locus_tag="ACHU9J_003683"
     CDS             5802..6995
                     /gene="tyrB"
                     /locus_tag="ACHU9J_003683"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1907131.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1693 a         1766 c         1904 g         1830 t
ORIGIN      
        1 aagttcttcg atagtatgga aggcattata taaaaggacc caatatttat tgggttcttt
       61 tttctctatc aatgctatta gcagggagat atatcaccag agtttaatgt gtgatttttt
      121 atttatcgtc gaacctggat tgtttatcat tggccttaac aaagttaacg gctaataaga
      181 ttatttccat cacttcgtga gagcttcatg ccttgagagg atctcaattt tcttttgcaa
      241 tgagacaggc gcttcctgtt gttatggtat agtaccccgc tattgagcct cctgaatagt
      301 gatgctgaat aacataaccc catgatatat cgataaaata atctctacat ttgaaaatgc
      361 acggtaattc tgaaatgcaa aaaatcaacc aaaccagcgc aatgcctgaa aaaactgacg
      421 ttcactggag tggtcggttt agcgttgcac caatgctcga ctggacggac agacattgcc
      481 gctatttctt gcgtctgctt tcccgcaata cgttgctgta taccgaaatg gtgaccacag
      541 gggcgattat tcacggtaaa ggtgattacc tggcgtacag tgaagaagaa catccggtag
      601 cgttgcaact cggcggtagc gatccggcgg cgctggcaca gtgtgcgaag ctggcagaag
      661 cgcgtggata tgatgagatc aacctgaatg tcggctgccc gtctgaccgg gtgcagaacg
      721 gcatgtttgg tgcgtgtctg atgggtaatg cgcagctggt tgccgactgc gtgaaagcga
      781 tgcgcgatgt ggtgtcgatt ccagtgacgg tgaaaacgcg tattggcatc gatgaccagg
      841 acagctatga atttctctgc gatttcatca acaccgtttc cggcaaaggc gagtgtgaga
      901 tgtttatcat ccacgcacgt aaagcctggc tttcggggtt aagcccgaaa gaaaaccgtg
      961 aaatcccgcc gctcgattat ccgcgtgtgt atcaactgaa gcgtgacttt ccgcatctga
     1021 caatgtcgat taacggtggt atcaagtcgc tggaagaggc caaagcacac ctgcaacata
     1081 tggatggcgt gatggtcggg cgcgaggcgt atcagaatcc gggtattctg gcggcggtag
     1141 accgggagat ctttggttcc tcggataccg atgccgatcc ggtggcggta gtgcgcgcca
     1201 tgtatccgta cattgagcgt gaactcagcc aggggacgta tctcggccat attacccggc
     1261 atatgttggg cttgttccag ggtattcctg gcgcgcggca gtggcggcgt tatttaagtg
     1321 aaaatgccca taaagcgggt gcagacatta atgtgctgga acacgcgctc aaactggtgg
     1381 cggataagcg ttaacttttc accaaaaagt agtcaaattc accacgccct gcgcaccgtc
     1441 gcggggcgtt ttgctgttaa atcaatagat tatttttggc atgattcttg taatgccagc
     1501 aagagatttc atatttggga gagcatcatg ctggaactac tttttgtgat tggctttttt
     1561 gtcatgctga tggtcaccgg cgtttcgttg ctgggcatta tcgccgcgct ggttgtggcg
     1621 acggccatta tgttcctcgg cggtatgctg gcattgatga ttaagttgct gccgtggtta
     1681 ctactggcga ttgcggtggt gtgggttatt aaggcgatta aagcaccaaa agtgccgaaa
     1741 tatcagcgtt atgaccgctg gcgttactaa gggattgtgc ggatgatcac aacctaaggt
     1801 tttatcctta gaacaaaata ggaattgata atcaaatctg tcactattgc gcctctaaca
     1861 gattcatcgt gctgtaccct acatacagcc gaactataaa aagaaagggc ttcccaggtg
     1921 gaagccctat ttcttttatg gaatcaacag gctggaacct tgcgtcgccc ggctttccag
     1981 aatctcatgc gcacgctgcg catccttcag cggatatttc tgctgctcgg cgacatcgac
     2041 cttaatcaca ccgctggcaa tcaaagagaa cagttcatta ctggcctcgg ttaattcctc
     2101 ccgcgtggtg atatagcctt gcagggaagg gcgtgtcaca tacaacgagc ctttttgatt
     2161 gagaatgcct aagttcacac cggtaaccgc acctgatgag ttgccaaaac tgaccattaa
     2221 gccgcggcgt tgcaggcaat ccagcgaccg ttcccaggtg tctctgccca cggaatcgta
     2281 caccacgcgc actttcttac cgccggtgat ctcttttaac cgctcgacca gatcctcttc
     2341 acgatagtta ataacctgcc acgcgcccgc ttttagcgcg ctctgcgctt tttgcgcggt
     2401 tcctacggtg ccgataagtt tcgcgcccag ggcttttgcc cactggcagg caattaagcc
     2461 aacgccgcca gccgctgcgt ggaacaggaa ctgttcatcg ggtttaattt cataggtttt
     2521 gcgcagcaga taataaaccg ttaagccttt caggaaggat gccgcagctt gctcaaaaga
     2581 aattgccgca ggcagaatcg ccgctttatc cgcattaatg ttatgcacag agctgtaagc
     2641 gcctaacgcc gactgcgcat agactacacg atcgcctgcc ttaatatgct ttacaccact
     2701 gccgacttta ctcacgatgc ctgccgcttc ggtacctaat ccgctgggta gtgatggcgg
     2761 cgggtaaagg ccgctgcgga tatacgtgtc gataaaattg atgccgatgg ctttattttc
     2821 gacctggatt tcattctctg ccggatcggc aggagtgaac tctacggctt gaagtacttc
     2881 cgggccaccg tgcttgtgaa attcaattcg tgttgccatg tgtcctccag aacgtatcgt
     2941 cagggtctgc ttcatatggt aaagtttcga cccattcttt atctcggtaa ctccattcac
     3001 tatggcagga aataaaccct tcaacaaaca gcaggctgaa ccccgcgaac gcgatccaca
     3061 agttgccggg ctgaaagtgc ctccgcactc gatcgaagcg gagcagtcgg tgttgggcgg
     3121 tttaatgctg gataacgaac gctgggacga tgtagccgag cgtgtggtgg cagacgattt
     3181 ttacacccgc ccacaccgcc atatctttac tgaaatggcg cgtttgcagg aaagcggtag
     3241 tcctatcgat ctgattaccc ttgcggaatc gctggaacgc caggggcaac tcgatagcgt
     3301 cggtggtttt gcttatctgg cagagctgtc aaaaaatacg ccaagtgcgg cgaacatcag
     3361 tgcttatgct gacatcgtgc gtgaacgtgc cgttgtccgt gagatgatct cggttgcgaa
     3421 tgagattgct gaagccggtt ttgatccgca ggggcgtacc agcgaagatc tgctggacct
     3481 tgctgaatcc cgcgtcttta aaattgccga aagtcgtgca aacaaagacg aagggccgaa
     3541 gaacatcgcc gatgtgctcg acgcaaccgt ggcgcgtatt gagcagttgt ttcagcagcc
     3601 acacgatggc gttaccggag taaacaccgg ttatgacgat ctcaacaaaa aaaccgctgg
     3661 cttgcagccg tcggatttga tcatcgtcgc cgcgcgtccg tcgatgggta aaacaacatt
     3721 tgcgatgaac ctcgtcgaaa acgcggcgat gttgcaggat aaaccagtac ttatcttctc
     3781 gctggagatg ccttcagaac agattatgat gcgttctctg gcgtcgctgt cgcgcgttga
     3841 ccagactaaa atccgtaccg ggcagctcga tgatgaagac tgggcgcgca tttccggcac
     3901 catgggtatt ttgctcgaaa aacgcaatat ctatatcgat gactcctccg gcctgacgcc
     3961 aacggaagtg cgttcccgcg cacgccgtat tgcccgtgaa cacggcggca tcgggcttat
     4021 catgatcgac tacctgcaac tgatgcgcgt accggcgctt tccgataacc gtacgctgga
     4081 aattgcagaa atctctcgct cgctgaaagc actggcgaaa gaactgaacg tgccggtggt
     4141 ggcgctgtcc cagttgaacc gttctctgga acaacgtgcc gacaaacgcc cggtcaactc
     4201 cgacctgcgt gaatctggct ctatcgagca ggatgcggac ttgattatgt ttatctatcg
     4261 tgatgaggtg tatcacgaaa acagtgattt aaaaggcatc gcggaaatta ttatcggtaa
     4321 acaacgtaac ggcccaatcg ggacggtacg cctgaccttt aacggtcaat ggtcgcgctt
     4381 cgacaactat gcggggccgc agtacgacga cgaataataa ttattttatg aattaggtaa
     4441 ttaaagcaaa cacttatcaa ggaacacaaa tgcaagcggc aactgttgtg attaaccgcc
     4501 gcgctctgcg acacaacctg caacgtcttc gtgaactggc ccctgccagt aaaatggttg
     4561 cggtggtgaa agcgaacgct tatggtcacg gtcttcttga gaccgcgcga acgctccccg
     4621 atgctgacgc ctttggcgta gcccgtctcg aagaagctct gcgactgcgt gcggggggaa
     4681 tcaccaaacc tgtactgtta ctcgaaggct tttttgatgc cagagatctg ccgacgattt
     4741 ctgcgcaaca ttttcatacc gccgtgcata acgaagaaca gctggctgcg ctggaagagg
     4801 ctagcctgga cgagccggtt accgtctgga tgaaactcga taccggtatg caccgtctgg
     4861 gcgtaaggcc ggaacaggct gaggcgtttt atcatcgcct gacacagtgc aaaaacgttc
     4921 gtcagccggt gaatatcgtc agccattttg cgcgcgcgga tgaaccaaaa tgtggcgcaa
     4981 ccgagaaaca actcgctatc tttaatacct tttgcgaagg caaacctggt caacgttcca
     5041 ttgccgcgtc gggtggcatt ctgctgtggc cacaatcgca ttttgactgg gtgcgcccgg
     5101 gcatcattct ttatggcgtc tcgccgctgg aagatcgctc catcggtgcc gattttggct
     5161 gtcagccagt gatgtcacta acctccagcc tgattgccgt gcgtgagcat aaagccggag
     5221 agcctgttgg ttatggtgga acctgggtaa gcgaacgtga tacccgtctt ggcgtagtcg
     5281 cgatgggcta tggcgatggt tatccgcgcg ccgctccatc cggtacgcca gtgctggtga
     5341 acggtcgcga agtaccgatt gtcggacgcg tggcgatgga tatgatctgc gtagacttag
     5401 gtccacaggc gcaggacaaa gccggggatc cggtcatttt atggggcgaa ggtttgcccg
     5461 tagaacgtat cgctgaaatg acaaaagtga gcgcttacga acttattacg cgcctgactt
     5521 caagggtcgc gatgaaatac gtggattaat cgttctgtaa tatttgattg tctgtgccgg
     5581 atgcggcgtg aatgccttat ccggccaata aaatcctaaa aattcaataa gttgatgttc
     5641 tttcatgctc ttataaaggt cgtgcctctg gcggatgttc gtttgtcatg agtctcactc
     5701 tgttgctaat tgccgttcgc tcctgaacat ccactcgatc ttcgccttct tccggtttat
     5761 tgtgttttaa ccacctgccc gtaaacctgg agaaccatcg cgtgtttcaa aaagttgacg
     5821 cctacgctgg cgacccgatt cttacgctta tggagcgttt taaagaagac cctcgcagcg
     5881 acaaagtgaa tttaagtatc ggtctgtact acaacgaaga cggaattatt ccacaactga
     5941 aagccgtggc ggaggcggaa gcgcgcctga atgcgcagcc ccatggcgct tcgctttatt
     6001 taccgatgga agggcttaac agctatcgcc atgccattgc gccgctgctg tttggtgcgg
     6061 accatccggt actgcaacaa cagcgcgtag caaccattca aacccttggc ggctcagggg
     6121 cattgaaagt gggcgcggat ttcctgaaac gctacttccc ggaatcaggc gtctgggtca
     6181 gcgatcctac ctgggaaaac cacgtagcaa tattcgccgg ggctggattc gaagtgagta
     6241 cttacccctg gtatgacgaa gcgactaaca gcgtgcgctt taatgacctg ttggcgacgc
     6301 tgaaaacatt acctgcccgc agtattgtgt tgctgcatcc atgttgccac aacccaacgg
     6361 gtgccgatct cactaatgat cagtgggatg cggtgattga aattctcaaa gcccgcgagc
     6421 ttatcccatt cctcgatatt gcctatcaag gatttagtgc cggtatggaa gaggatgcct
     6481 acgctattcg cgccattgcc agcgctggat tacccgctct ggtgagcaat tcgttctcga
     6541 aaattttctc cctttacggc gagcgcgtcg gcggactttc tgttctgtgt gaagatgccg
     6601 aagccgctgg ccgcgtactg gggcaattga aagcaacagt tcgccgcaac tactccagcc
     6661 cgccgaattt tggtgcgcag gtggtggctg cagtgctgaa tgacgaggca ttgaaagcca
     6721 gctggctggc ggaagtagaa gagatgcgta ctcgcattct ggcaatgcgt caggaattgg
     6781 tgaaggtatt aagcacagag atgccagaac gcaatttcga ttatctgctt aatcagcgcg
     6841 gcatgttcag ttataccggt ttaagtgccg ctcaggttga ccgactacgt gaagaatttg
     6901 gtgtctatct catcgccagc ggtcgcatgt gtgtcgccgg gttaaatacg gcaaatgtac
     6961 aacgtgtggc aaaggcgttt gctgcggtga tgtaatgcag gaaagcaggc tggagttacc
     7021 cagcctgcag tgaaattaaa ctgtcgtcgc tttcactatt tctttgtaga tgattttttt
     7081 gatgccgttg ttctacgtga gagataataa aacgttgtca gttcttttat tgttaagttt
     7141 atcccaatta tctggaattc ctttccctat tttttcgtgt ggagtaataa ttt
//
LOCUS       DAZPJP010000203         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPJP010000203 DAZPJP010000000
VERSION     DAZPJP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.204"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHU9J_003684"
     CDS             <1..435
                     /locus_tag="ACHU9J_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1907132.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHU9J_003685"
     CDS             518..1420
                     /locus_tag="ACHU9J_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1907133.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHU9J_003686"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHU9J_003686"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1907134.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHU9J_003687"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHU9J_003687"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1907135.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHU9J_003688"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHU9J_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1907136.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHU9J_003689"
     CDS             complement(5330..6037)
                     /locus_tag="ACHU9J_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1907137.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHU9J_003690"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHU9J_003690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1907138.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHU9J_003691"
     CDS             complement(6895..7098)
                     /locus_tag="ACHU9J_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1907139.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1855 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZPJP010000204         7116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPJP010000204 DAZPJP010000000
VERSION     DAZPJP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.205"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            166..1599
                     /gene="cscA"
                     /locus_tag="ACHU9J_003692"
     CDS             166..1599
                     /gene="cscA"
                     /locus_tag="ACHU9J_003692"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1907140.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1607..2602)
                     /locus_tag="ACHU9J_003693"
     CDS             complement(1607..2602)
                     /locus_tag="ACHU9J_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1907141.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2833..3051
                     /locus_tag="ACHU9J_003694"
                     /pseudo
     CDS             2833..3051
                     /locus_tag="ACHU9J_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3069..4397
                     /gene="dsdA"
                     /locus_tag="ACHU9J_003695"
     CDS             3069..4397
                     /gene="dsdA"
                     /locus_tag="ACHU9J_003695"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1907142.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4505..6043)
                     /gene="emrY"
                     /locus_tag="ACHU9J_003696"
     CDS             complement(4505..6043)
                     /gene="emrY"
                     /locus_tag="ACHU9J_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1907143.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6043..>7116)
                     /gene="emrK"
                     /locus_tag="ACHU9J_003697"
     CDS             complement(6043..>7116)
                     /gene="emrK"
                     /locus_tag="ACHU9J_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1907144.1"
                     /translation="AYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTN
                     YVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQS
                     LEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQP
                     QVVEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVN
                     ANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWI
                     KIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPELASTVTSMPAYTSK
                     ALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1946 a         1708 c         1655 g         1807 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
      181 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
      241 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
      301 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
      361 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
      421 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
      481 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgattgtgc aggtaatgac
      541 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
      601 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
      661 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
      721 atcctgcttt atcgcggcag ttcattgcgt gaatggacct tcgatcgcgt actggcccac
      781 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
      841 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
      901 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
      961 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
     1021 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
     1081 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
     1141 cttctacaac gcccggttca cgaagctgag tcgttacgcc agcagcatca atctgtctct
     1201 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
     1261 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
     1321 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
     1381 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
     1441 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
     1501 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
     1561 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
     1621 ggatcaacag cggacaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
     1681 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
     1741 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
     1801 tacgtacccc cttcgccagg agaacctgat aagcgacaaa ggctgcacga tcgttaccac
     1861 atatcagaac atcaaaatct ggtttgcccg atttgaagtg ggcattgagt aaacttgcga
     1921 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
     1981 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
     2041 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
     2101 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
     2161 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
     2221 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
     2281 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
     2341 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
     2401 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
     2461 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
     2521 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gccaggcgtg
     2581 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
     2641 aacagacgtc actggcgaga tccaacgccc tatcacctga cacagcaata caataaaaaa
     2701 taacaataat tcccggacaa ttgtccccaa ttccgcctct gttctcgcca acgagtaacc
     2761 atataggcaa gtaagtaaaa catgccaact ggcatcgcca acctggagaa gaaaatataa
     2821 atatggatca ttgtgctgcc gctgtatccc gacatcagcc cggaaattat tgcgattgct
     2881 atcggttcag gtgcaattgg ctgcacgatc gttacggact cgcttttctg gctagtgaag
     2941 caatattgcg gcgctacgct caatgaaaca tttaaatact atacgacagc gacatttatc
     3001 gcttcagtcg tcgctctggc gggcacattc ctgctgtcat ttatcatcta agcgcaaaga
     3061 gacgtactat ggaaaacgct aaaatgaact cgctcatcgc ccagtatccg ttggtaaagg
     3121 atctggttgc tcttaaagaa accacctggt ttaatcctgg cacgacctca ttggctgaag
     3181 gtttacctta tgttggcctg accgaacagg atgttcagga cgcccatgcg cgcttatccc
     3241 gttttgcacc ctatctggca aaagcatttg ctgaaactgc tgccactggg gggattattg
     3301 aatcagaact ggttgccatt ccagctatgc aaaaacggct ggaaaaagaa tatcagcaac
     3361 cgatcagcgg gcaactgtta ctgaaaaaag atagccattt gcccatttcc ggctccataa
     3421 aagcacgcgg cgggatttat gaagtcctgg cacacgcaga aaaactggct ctggaagcgg
     3481 ggttgctgac gcttgatgat gactacagca aactgctttc tccggagttt aaacagttct
     3541 ttagccaata cagcattgct gtgggctcaa ccggaaatct ggggttatca atcggcatta
     3601 tgagcgcccg cattggcttt aaggtgacag ttcatatgtc tgctgatgcc cgggcatgga
     3661 aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga atatgagcaa gattatggtg
     3721 ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc gaactgtttc tttattgatg
     3781 acgaaaattc ccgcacgttg ttccttgggt attccgtcgc tggccagcgt cttaaagcgc
     3841 aatttgccca gcaaggccgt atcgtcgatg ctgataaccc tctgtttgtc tatctgccgt
     3901 gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct taaactggcg tttggcgatc
     3961 atgttcactg cttttttgcc gaaccaacgc actccccttg tatgttgtta ggcgtccata
     4021 caggattaca cgatcagatt tctgttcagg atattggtat cgacaacctt accgcagcgg
     4081 atggccttgc agttggtcgc gcatcaggct ttgtcgggcg ggcaatggag cgtctgctgg
     4141 atggcttcta tacccttagc gatcaaacca tgtatgacat gcttggctgg ctggcgcagg
     4201 aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat ggccggacct cagcgcgtat
     4261 gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc agaacaactg cgtaatacca
     4321 ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga agaagagatg aatcaatatc
     4381 tggcaaaagg ccgttaataa cgtttcaacg cagcatcgca atcctttccc tgggtgagcg
     4441 atgctgccga tggcgcagac ttaagatccc cggtcttacc cgctataacc ccctcctttt
     4501 atcatcaccc aacgcctttc gctgtaaacg gcggtttcgc aaaccaaacc aaaaccgtaa
     4561 gtaagataaa agcaatagcc gccatacgga aaatctcatt tgcagaaata gaaagtgact
     4621 gctgggttat ttcattatta atttcattaa gaactcctga aagcgaacca taatatttat
     4681 ccataatttg tgacgatgaa ttaaacacgg ggttaaattg atcgatggtt gctgtcaact
     4741 gactatggtg taacgattcg cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg
     4801 atcctgacaa ggtacgaaaa aaattactca tactcgaggc attggcaaat ttattatctg
     4861 gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct
     4921 gaaaaaactg cggcaaaatg atgcctgtaa aatcaatcgt tggcataaat gtcacagaac
     4981 gccagtaata gcaaaccgca tacatcaaaa aactaaatgt cactaacaac cgcatgtcta
     5041 ttttgttgcc ataacgtcct atcaaaggtg aaattaatag tggcatgatg ccgatgggcg
     5101 cataagcaag tccggcccat atcgcattat accccatcgt ttcctggagt aactgcggca
     5161 taaggacgat cgctccagag taaaataaat acgcgcatgt gatactcaca ataccaatgg
     5221 tgaagttacg ggacttaaac aaactgagat caagaatcgg gttctctgag gtcgactccc
     5281 aaatgactaa agagatcaga gaaataactg atactactgt taatattatt attgtactcg
     5341 agttgaacca atccagatcg cgccctttat caagcataat ttgcaagcca ccaacaccga
     5401 gcactaacag ggtcagtcct ggtagattca ttttgaccgg tgaagtctca gtttctcttc
     5461 ctttaagtaa ggttaagcat aatgtcagga cgataatccc catagggaca ttgattaaaa
     5521 atatccaacc ccagctaaag ttatcacaaa tataaccgcc caatatcggc ccacatatcg
     5581 gagcgataat cacggtcatt gaccataatg ccagagcaaa tgttcttttt tctggcggat
     5641 aattccttaa taataaactc tgtgacagtg gaattaacgg ccccgccatt aacccctgaa
     5701 cgactctaaa aaatatcagc acatcaagat tggtcgatag gctacacatt aatgaagaca
     5761 gcgaaaaaaa ggttactgaa agtaaaaata atcttaattc gcctattctt tgtgccaacc
     5821 tgccagtaac agggatcgca atggcatttg ctacaccaaa cgaggtgata acccaggtgc
     5881 cttcgtctgt tgatgctccc agaaagccag atattgtcgg tattgcgacg ttagaaatag
     5941 tggaatccaa catttgcata aatgtcgcta atgacaatgc aatagtgacg caccataacg
     6001 tcccaccggt taatggtgcc ggagttgatt tagtgattgc cattaaagtt gtccattatg
     6061 cgaaataatg ttgctaattt ctttttctat cggactggta tcgataacta aagccttact
     6121 ggtataagcc ggcatggagg tcacggttga agccagctca ggcatctcag caatgtcttc
     6181 gtccttcgta tcaatagttg cagtcatcga taaaccaatg cgcaaggggt gttccatgag
     6241 ttcttttgga tcaagagaaa cttcaaccgg tacacgctga acgattttga tccagttccc
     6301 tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg gttcccatat tgatccctgt
     6361 cacccgacca tgaaacacaa cattttcacc ataaagatcg ctgataatat tgactgattg
     6421 accaatccgt acatccgtga gttgtgtttc tttaaagttg gcattaaccc acatttgacg
     6481 tgccggtacg acagccatta acgattgtcc gggactcact gtttcgccga cctgaacact
     6541 tcgctgggca atatagccag taaccggact ccttatatcc gtacgtttaa gcgccaacca
     6601 ggcttctttg gttgcatccg ccgcttcaac tacttgtggc tgacggttta atggtgtgtt
     6661 cattactaaa gctttattcg ctttataagc ctggatagcg gcattcagtg ccgctttgct
     6721 acttattaac gtatctttgg tatgctccag cgtttctttt gaaataaccc cctgcttcgc
     6781 taaaggcaca cgacggttat aatcttctaa ggattgttga tactgaatac gtgctgaagc
     6841 gacttcggca ctgtattgtt tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt
     6901 tgccagatta tttttagctt tattgagtgc gatagtggca tcagttttat ccagtgaaac
     6961 taaaatgtca ccctgtcgaa cgtagttcgt atctttatga ttaactacag tgacactacc
     7021 tgagacttgt gcagaaattg gatctgcatt ccccgtgaca taggcgtcat ctgtactaat
     7081 catgtcttct aattccattg accaataggc ataggc
//
LOCUS       DAZPJP010000205         7059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPJP010000205 DAZPJP010000000
VERSION     DAZPJP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.206"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            485..1474
                     /gene="araF"
                     /locus_tag="ACHU9J_003698"
     CDS             485..1474
                     /gene="araF"
                     /locus_tag="ACHU9J_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR1907145.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1544..3058
                     /gene="araG"
                     /locus_tag="ACHU9J_003699"
     CDS             1544..3058
                     /gene="araG"
                     /locus_tag="ACHU9J_003699"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1907146.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3073..4059
                     /gene="araH"
                     /locus_tag="ACHU9J_003700"
     CDS             3073..4059
                     /gene="araH"
                     /locus_tag="ACHU9J_003700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1907147.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4226..5026
                     /gene="otsB"
                     /locus_tag="ACHU9J_003701"
     CDS             4226..5026
                     /gene="otsB"
                     /locus_tag="ACHU9J_003701"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1907148.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5001..6425
                     /gene="otsA"
                     /locus_tag="ACHU9J_003702"
     CDS             5001..6425
                     /gene="otsA"
                     /locus_tag="ACHU9J_003702"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR1907149.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6432..6860)
                     /gene="uspC"
                     /locus_tag="ACHU9J_003703"
     CDS             complement(6432..6860)
                     /gene="uspC"
                     /locus_tag="ACHU9J_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1907150.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1794 a         1642 c         1824 g         1799 t
ORIGIN      
        1 ttttcatttt tgcgagatgg ctctcattat acgtgttctg ttaattaatg agcacagtga
       61 taattaatgg cgaaatgaat gacgtgcatt ttccacatct ttgagttgcg gttattacac
      121 catttcaaaa actcaacgcc aggtaatgcg gcctattgac tggttaaaaa gaagacatcc
      181 cgcatgggta ccaaagacaa caaggatttc caggctaatc ttatggatta atctgctgtg
      241 cattcgacaa tttgtctgac aaattggctt tcccttatgt cttttcccgc taaatttatg
      301 cacgttctca ctgtaattct gcgatgtgat attgctctcc tatggagaat taatttctcg
      361 ctaaaactat gtcaacacgg tcacttatct tttagataaa aggtaatgct ttgttttccg
      421 attaatttaa cgaaagtcat tcgtttttgc cctacacaaa acgacactaa agctggagag
      481 aaccatgcac aaatttacta aagccctggc agccattggt ctggcagccg ttatgtcaca
      541 atccgctatg gcggagaacc tgaagctcgg ttttctggtg aagcaaccgg aagagccgtg
      601 gttccagacc gaatggaagt ttgccgataa agccgggaag gatttagggt ttgaggttat
      661 taagattgcc gtgccggatg gcgaaaaaac attgaacgcg atcgacagcc tggctgccag
      721 tggcgcaaaa ggtttcgtta tttgtactcc ggaccccaaa ctcggctctg ccatcgtcgc
      781 gaaagcgcgt ggctacgata tgaaagtcat tgccgtggat gaccagtttg ttaacgccaa
      841 aggtaagcca atggataccg ttccgctggt gatgatggcg gcgactaaaa ttggcgaacg
      901 tcagggtcag gaactgtata aagagatgca gaaacgtggc tgggatgtca aagaaagcgc
      961 ggtgatggcg attaccgcca acgaactgga tacggcacgt cgccgtacaa cgggttctat
     1021 ggatgcgctg aaagcggccg gattcccgga aaaacaaatt tatcaggtac ctaccaaatc
     1081 taacgacatc ccgggggcat ttgacgctgc caactcaatg ctggttcaac atccggaagt
     1141 taaacattgg ctgatcgtcg gtatgaacga cagcaccgtg ctgggcggcg tacgcgcgac
     1201 ggaaggtcag ggctttaaag ctgccgatat catcggcatt ggcattaacg gtgtggatgc
     1261 gttgagcgaa ctgtctaaag cacaggcaac cggcttctac ggttccctgc tgccaagccc
     1321 ggacgtacat ggctataaat ccagcgaaat gctttacaac tgggtagcaa aagacgttga
     1381 accgccaaaa tttaccgaag ttaccgacgt ggtgctgatc acgcgtgaca actttaaaga
     1441 agaactggag aaaaaaggtt taggcggtaa gtaatttgcc ggaaaaattc ccctctgcat
     1501 gatgcagagg gggtgtgaac gaccagtgat tcacggagac gttatgcaac agtctacccc
     1561 gtatctctca tttcgcggca tcggtaaaac gtttcccggc gttaaggcgc tgacggatat
     1621 tagttttgac tgctatgccg gtcaggttca tgcgttgatg ggtgaaaatg gcgcaggaaa
     1681 atcaactctc ttaaaaatcc tcagcggcaa ctatgcgcca accacgggtt ctgtagtgat
     1741 taatgggcag gaaatgtcct tttccgacac gaccgcagca cttaacgcgg gtgtggcgat
     1801 tatttaccag gaactgcatc tcgtgccgga aatgaccgtc gcggaaaaca tctatctcgg
     1861 ccagctgccg cataaaggcg gcattgtgaa tcgctcattg ctgaattatg aggcgggttt
     1921 acaacttaaa catcttggta tggatattga cccggacacg ccgctgaaat atctctccat
     1981 tggtcagtgg cagatggttg aaatcgccaa ggcgctggcg cgtaacgcta aaattatcgc
     2041 ctttgatgag ccaaccagct ccctttctgc ccgcgaaatc gacaatcttt tccgcgttat
     2101 tcgtgaactg cgaaaagagg ggcgggtgat cttatacgtt tctcaccgta tggaagaaat
     2161 atttgccctc agcgatgcca tcactgtctt taaagatgga cgttatgtca aaacctttac
     2221 cgatatgcag caggttgacc acgacgcgct ggtgcaggcg atggtcgggc gcgacattgg
     2281 cgatatctac ggctggcaac ctcgtagtta tggcgaggag cgcctgcgtc ttgatgctgt
     2341 gaaagcacca ggcgtgcgta cgccaataag tctggcggtt cgcagtggtg aaattgttgg
     2401 gctgtttggt ctggtagggg cggggcgtag cgaattaatg aaaggcttgt ttggcgggac
     2461 gcaaatcacc gccggtcagg tttatatcga ccaacagccg atcgatattc gtaaaccgag
     2521 ccacgccatt gccgcaggca tgatgctctg cccggaagat cgcaaagcgg aaggcattat
     2581 tcccgtgcac tccgttcgcg acaatatcaa catcagtgcc agacgcaaac atgtgctcgg
     2641 cggttgtgta atcaacaacg gttgggaaga aaacaatgcc gatcagcaca ttcgttcgct
     2701 caacatcaaa acgccgggcg ctgagcaact gatcatgaat ctctcaggcg gaaatcagca
     2761 aaaagccatt ctgggccgct ggttatcgga agagatgaag gtcattttgc tggatgaacc
     2821 tacgcgcagc attgatgttg gtgctaagca cgaaatatat aacgtaattt atgcgctggc
     2881 ggcgcagggt gtggcggtgc tgtttgcctc cagcgactta cctgaagtcc tcggcgttgc
     2941 cgaccggatt gtggtgatgc gggaaggtga aatcgccggt gaattgttac acgagcaggc
     3001 agatgagcgt caggcactga gccttgcgat gcctaaagtc agccaggcag ttgcctgagt
     3061 aaggagagta tgatgtcttc tgtttctaca tcgggttctg gcgcacctaa gtcgtcattc
     3121 agcttcgggc gtatttggga tcagtacggc atgctggtgg tgtttgcggt gctctttatc
     3181 gcctgtgcca tttttgtccc aaattttgcc accttcatta atatgaaagg gttgggcctg
     3241 gcaatttcca tgtcggggat ggtggcgtgt ggcatgttgt tctgccttgc ttccggtgac
     3301 tttgaccttt ctgtcgcctc cgtaattgcc tgtgcgggtg tcaccacggc ggtggttatc
     3361 aacctgactg aaagcctgtg gattggcgtg gcagcggggt tgctgcttgg cattctctgt
     3421 ggcctggtca atggctttgt tatcgccaaa ctgaaaataa atgctctgat cacaacactg
     3481 gcaacgatgc agattgttcg aggtctggcg tacatcattt cagacggtaa agcggtcggt
     3541 atcgaagatg aaagcttctt tgcccttggt tacgctaact ggttcggtct gcctgcgcca
     3601 atctggctca ccgtcgcgtg tctgattatc tttggtttgt tgctgaataa aaccaccttt
     3661 ggtcgtaaca ccctggcgat tggcgggaac gaagaggctg cgcgtctggc gggtgtaccg
     3721 gttgttcgca ccaaaattat tatctttgtt ctctctggcc tggtatctgc gatagccgga
     3781 attattctgg cttcacgtat gactagtggg cagccaatga cgtcgattgg ttatgagctt
     3841 attgttatct ccgcctgcgt tttaggtggc gtttctctga aaggtggcat cggaaaaatc
     3901 tcatatgtgg tggcgggtat cttaatttta ggcaccgtgg aaaacgccat gaacctgctt
     3961 aatatttctc ctttcgcgca gtacgtggtt cgcggcttaa tcctgctggc agcggtgatc
     4021 ttcgaccgtt acaagcaaaa agcgaaacgc actgtctgat gcttttttcc acaacaattt
     4081 aacgtttttt cccaccacag ccagccgcca caacggttgg ctgttcttca ttgcaaatgg
     4141 cgacccccgt cacactgtct atacttacat gtctgtaaag cgcgttctgc gcaaaacaat
     4201 aagaaaagag aaggaggaga gccgggtgac agaaccgtta accgaaaccc ctgaactatc
     4261 cgcgaaatat gcctggtttt ttgatcttga tggaacgctg gcggaaatca aaccgcatcc
     4321 cgatcaggtc gtcgtgcctg acaatattct gcaaggacta cagctactgg caaccgcaag
     4381 tgatggtgca ttggcattga tatcagggcg ctcaatggtg gagcttgacg cactggcaaa
     4441 accttatcgc ttcccgttag cgggcgtgca tggggcggag cgccgtgaca tcaatggtaa
     4501 aacacatatc gttcatctgc cggatgcgat tgcgcgtgat attagcgtgc aactgcatac
     4561 agtcatcgct cagtatcccg gcgcggagct ggaggcgaaa gggatggctt ttgcgctgca
     4621 ttatcgtcag gctccgcagc atgaagacgc attaatgaca ttagcgcaac gtattactca
     4681 gatctggcca caaatggcgt tacagcaggg aaagtgtgtt gtcgagatca aaccgagagg
     4741 taccagtaaa ggtgaggcaa ttgcagcttt tatgcaggaa gctcccttta tcgggcgaac
     4801 gcccgtattt ctgggcgatg atttaaccga tgaatctggc ttcgcagtcg ttaaccgact
     4861 gggcggaatg tcagtaaaaa ttggcacagg tgcaactcag gcatcatggc gactggcggg
     4921 tgtgccggat gtctggagct ggcttgaaat gataaccacc gcattacaac aaaaaagaga
     4981 aaataacagg agtgatgact atgagtcgtt tagtcgtagt atctaaccgg attgcaccac
     5041 cagacgagca cgccgccagt gccggtggcc ttgccgttgg catactgggg gcactgaaag
     5101 ccgcaggcgg actgtggttt ggctggagtg gtgaaacagg gaatgaggat cagccgctaa
     5161 aaaaggtgaa aaaaggtaac attacgtggg cctcttttaa cctcagcgaa caggaccttg
     5221 acgaatacta caaccaattc tccaatgccg ttctctggcc cgcttttcat tatcggctcg
     5281 atctggtgca atttcagcgt cctgcctggg acggctatct acgcgtaaat gcgttgctgg
     5341 cagataaatt actgccgctg ttgcaagacg atgacattat ctggatccac gattatcacc
     5401 tgttgccatt tgcgcatgaa ttacgcaaac ggggagtgaa taatcgcatt ggtttctttc
     5461 tgcatattcc tttcccgaca ccggaaatct tcaacgcgct gccgacatat gacaccttgc
     5521 ttgaacagct ttgtgattat gatttgctgg gtttccagac agaaaacgat cgtctggcgt
     5581 tcctggattg tctttctaac ctgacccgcg tcacgacacg tagcgcaaaa agccatacag
     5641 cctggggcaa agcatttcga acagaagtct acccgatcgg cattgaaccg aaagaaatag
     5701 ccaaacaggc tgccgggcca ctgccgccaa aactggcgca acttaaagcg gaactgaaaa
     5761 acgtacaaaa tatcttttct gtcgaacggc tggattattc caaaggtttg ccagagcgtt
     5821 ttctcgccta tgaagcgttg ctggaaaaat atccgcagca tcatggtaaa attcgttata
     5881 cccagattgc accaacgtcg cgtggtgatg tgcaagccta tcaggatatt cgtcatcagc
     5941 tcgaaaatga agctggacga attaatggta aatacgggca attaggctgg acgccgcttt
     6001 attatttgaa tcagcatttt gaccgtaaat tactgatgaa aatattccgc tactctgacg
     6061 tgggcttagt gacgccactg cgtgacggga tgaacctggt agcaaaagag tatgttgctg
     6121 ctcaggaccc agccaacccg ggcgttcttg ttctttcgca atttgcggga gcggcaaacg
     6181 agttaacgtc ggcgttaatt gttaacccct acgatcgtga cgaagttgca gctgcgctgg
     6241 atcgtgcatt gactatgtcg ctggcggaac gtatttcccg tcatgcagaa atgctggacg
     6301 ttatcgtgaa aaacgatatt aaccactggc aggagtgctt cattagcgac ctaaagcaga
     6361 tagttccgcg aagcgcggaa agccagcagc gcgataaagt tgctaccttt ccaaagcttg
     6421 cgtaggagcg gttaatctcc cgtaagtgga actaacagca catcgacctc acttgaggca
     6481 atcactcttt tcgccgagca ggatgctcgc gaaaagaaac tgtgattgtg attaccgcaa
     6541 atcaccaaat cgaaatgatg tttgtgacat acttccagaa tatgttcgct taactcaccg
     6601 taggcaataa acgtcttgtc aacgggatac cccgcatcct gaattaactt atcaagaaag
     6661 ctttgcgttt cttcttgcat cacgctgcgt aaatcttcaa gcatcggcgc ggctaattga
     6721 ttgtacattt ccgggtcgga agcgagagta attaaactga tgtgtccctt tactggcctg
     6781 gcgatagata ccgcttttgc cagcagttgc tgactttccg gtgtaaccgc aacagcgaca
     6841 agaatattgc tatagctcat aacctgttcc ttattctgtg aacttcaggt gacattaaag
     6901 ctttactcct aatggagaaa cgacgcaatc ccaactcggt caaacaggta aatattagtc
     6961 tgattattag taaagacaat taatcaatca ctcccggtga tcatataatt tcaaatgtaa
     7021 ttataagaaa atagtgtact gattctccgc attaaacaa
//
LOCUS       DAZPJP010000206         6851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPJP010000206 DAZPJP010000000
VERSION     DAZPJP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.207"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHU9J_003704"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHU9J_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1907151.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHU9J_003705"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHU9J_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1907152.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHU9J_003706"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHU9J_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR1907153.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRVFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHU9J_003707"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHU9J_003707"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR1907154.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHU9J_003708"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHU9J_003708"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1907155.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..>6851)
                     /gene="cas3"
                     /locus_tag="ACHU9J_003709"
     CDS             complement(4156..>6851)
                     /gene="cas3"
                     /locus_tag="ACHU9J_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR1907156.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPEL
                     KIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINT
                     LFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGS
                     EEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQE
                     APFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDS
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1660 a         1862 c         1622 g         1707 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa acgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca gtaaacttaa
     6841 aggatattta c
//
LOCUS       DAZPJP010000207         6826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPJP010000207 DAZPJP010000000
VERSION     DAZPJP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.208"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHU9J_003710"
     CDS             complement(155..667)
                     /locus_tag="ACHU9J_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1907157.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHU9J_003711"
     CDS             862..1626
                     /locus_tag="ACHU9J_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907158.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHU9J_003712"
     CDS             1577..2980
                     /locus_tag="ACHU9J_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1907159.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHU9J_003713"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHU9J_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHU9J_003714"
     CDS             4337..5080
                     /locus_tag="ACHU9J_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1907160.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHU9J_003715"
     CDS             5084..6304
                     /locus_tag="ACHU9J_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1907161.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHU9J_003716"
     CDS             6308..6814
                     /locus_tag="ACHU9J_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907162.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAZPJP010000208         6782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPJP010000208 DAZPJP010000000
VERSION     DAZPJP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.210"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(231..716)
                     /gene="sixA"
                     /locus_tag="ACHU9J_003717"
     CDS             complement(231..716)
                     /gene="sixA"
                     /locus_tag="ACHU9J_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1907163.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(919..3063)
                     /gene="fadJ"
                     /locus_tag="ACHU9J_003718"
     CDS             complement(919..3063)
                     /gene="fadJ"
                     /locus_tag="ACHU9J_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1907164.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3063..4373)
                     /gene="fadI"
                     /locus_tag="ACHU9J_003719"
     CDS             complement(3063..4373)
                     /gene="fadI"
                     /locus_tag="ACHU9J_003719"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1907165.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4553..4837)
                     /locus_tag="ACHU9J_003720"
     CDS             complement(4553..4837)
                     /locus_tag="ACHU9J_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1907166.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5209..6549
                     /gene="fadL"
                     /locus_tag="ACHU9J_003721"
     CDS             5209..6549
                     /gene="fadL"
                     /locus_tag="ACHU9J_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1907167.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1688 a         1816 c         1621 g         1657 t
ORIGIN      
        1 tatctgtatt aataaatgac aatgagtgtc aaacagacat tattcatttc aacatgtaac
       61 tttgggcaaa atcaagatac gcgcagaaga ataatttccg aatgaaagta gaaagtgttt
      121 tatttataga tggggagagt cttcgatagt agtgagattg acagatatcc gctgcctgat
      181 gagcaattat gcccaatgaa tctacctcat tgggcataat ttggaactca tcagatagct
      241 tttgccatct tcagattaca cgggctcatc tgccagttga atgtgccgtt tccgctttca
      301 tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat
      361 aactcggcaa ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca
      421 ccttcattgg tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt
      481 aactccggca gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt
      541 gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct
      601 ttcagccagt tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc
      661 gaatcactgg cggcatcaag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg
      721 caccgctttt gttaaccaga ttcgtccgtt ttctttcatc tgaaaccaga gaaagaaaac
      781 ggtggccggc attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg
      841 cgtaagtata gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt
      901 catatagctt tgaccttctt attgaaggtc agttgcagtt gttttccaaa aactttcccc
      961 acgcgcgccc atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc
     1021 cagtcgttgc attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg
     1081 tccaccgaga aatggcggaa aaccaatgcc aaataccgcg ccaatatccc cgtcacgcac
     1141 gctacggata acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca
     1201 ccgttcagca acctgcggtg cggagagtcg cccctgccct tgtgcgccaa tgagcgggta
     1261 aatggcggga tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc
     1321 ccgaccattt tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg
     1381 cgcgctaaaa cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc
     1441 gattcctacc tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc
     1501 ggcatcaatg tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta
     1561 aggcgctaag atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt
     1621 gccctgtttt ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg
     1681 aggaataatc tctaccagcg gcattttttc cacaggactg aagaaatgca ggccaataac
     1741 ttgctcaggt cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga
     1801 agcaaaaatg gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa
     1861 ttcgagattt tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc
     1921 cgtcgttccg gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg
     1981 gcgaactttg ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg
     2041 gttgatatct ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc
     2101 gccgcccatc aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc
     2161 actaccggga tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga
     2221 ttgcggcgtc atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt
     2281 gccctgcgct aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc
     2341 ttgagttttg tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg
     2401 ccccgccaga atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc
     2461 cagctcaaca gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag
     2521 ctttaatgcc tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact
     2581 gacgcctatc agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg
     2641 tacttcaggc aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc
     2701 cagcgccagc tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat
     2761 gggcaaagca tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc
     2821 ttgcgccgtt ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg
     2881 tttcgcggag ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat
     2941 ggcgcgcacc tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacatc
     3001 gatggtgatg atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc
     3061 cattattccg cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact
     3121 aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg
     3181 ccggtcgccg cgaagggatg cccgtaagca atcgaaccgc caagcacgtt aaatttgcta
     3241 tcgtccactt cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta
     3301 cccagcaact gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat
     3361 gtcagttcgc tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga
     3421 ccgagcaaca tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc
     3481 agcggcacca gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg
     3541 ccatcggtca gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc
     3601 gggcgcagct ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca
     3661 agcggttgtt tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct
     3721 gaccatgcct gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg
     3781 gtgatgccgt aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa
     3841 tattctgcta ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt
     3901 ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt
     3961 ttactgacgc caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg
     4021 atagttcccg ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa
     4081 gcgcggctga cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca
     4141 cgcgcaatgt tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc
     4201 acttcagcgg ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca
     4261 accgcgggaa tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta
     4321 acaatggcga tacgatcgcc ctggcgggta accagcggta aaacctgacc cataacactc
     4381 ccctgtaaaa aaataaataa agtggtctga cctgatcata gtcttaacta tttttttaca
     4441 tttagccaag cggagaaaaa ggaaagtggg agctatgaca cagtgaagac aggagaaaga
     4501 aaagaaaacg cccctgccag caactgacag gggcgaacgt acccgagagg aattaacgca
     4561 gacccagctg gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc
     4621 gcacaccgtc agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc
     4681 tacgggcttt ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca
     4741 cgcggctgta agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc
     4801 agcaaaccgg ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca
     4861 cccaaggtgc cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt
     4921 gttaattctg caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa
     4981 attcacccga atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta
     5041 gatcatattt gaaaaaagat ggaaacatac ttgcagcatt ccagctggtc cgacctatac
     5101 tctcgccact ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta
     5161 cagcacgtaa catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa
     5221 accctgttta caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg
     5281 tcggcaggct ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg
     5341 gaaggcgcaa ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg
     5401 tttgaccgcc cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc
     5461 ggaacgtctc cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt
     5521 ccgaacatgc actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc
     5581 tctaactatg gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt
     5641 acaactgacc ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca
     5701 tggagctttg gtcttggttt caacgccgtc tacgctcgcg cgaaaattga acgtttcgca
     5761 ggcgatctgg ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct
     5821 caagggcaag cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat
     5881 ctgaacggta accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa
     5941 aataaccgct atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac
     6001 agcagcgatc ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt
     6061 ggcgcaacgc aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt
     6121 tataaccgtg ttgatccaca gtgggcgatt cactatagcc tggcttacat cagctggagt
     6181 cagttccagc agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa
     6241 ggctttaaag atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg
     6301 accttccgta ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc
     6361 tccattccgg accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa
     6421 gatgcttcag tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa
     6481 ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac
     6541 gcgttctgat aacgcgttcg cctggataaa gtcacctgca tagcaggtga ctttaactcc
     6601 cccacttcac cgaaagtagt gtcctcattg cttacctcct gagttttgca aacagcctgt
     6661 tggcagcttg ccctttcaaa tcaataagcg gtatccacag acaaacctga agggaaaggc
     6721 atattttcag gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat
     6781 ac
//
LOCUS       DAZPJP010000209         6768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPJP010000209 DAZPJP010000000
VERSION     DAZPJP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.211"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHU9J_003722"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHU9J_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1907168.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHU9J_003723"
     CDS             complement(1360..1518)
                     /locus_tag="ACHU9J_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1907169.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHU9J_003724"
     CDS             2089..2238
                     /locus_tag="ACHU9J_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907170.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="ACHU9J_003725"
     CDS             complement(2983..3423)
                     /locus_tag="ACHU9J_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1907171.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="ACHU9J_003726"
     CDS             3641..4030
                     /locus_tag="ACHU9J_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907172.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="ACHU9J_003727"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="ACHU9J_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="ACHU9J_003728"
     CDS             complement(4585..5421)
                     /locus_tag="ACHU9J_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1907173.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..5696)
                     /locus_tag="ACHU9J_003729"
                     /pseudo
     CDS             complement(5421..5696)
                     /locus_tag="ACHU9J_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5771..6244)
                     /gene="dfrA14"
                     /locus_tag="ACHU9J_003730"
     CDS             complement(5771..6244)
                     /gene="dfrA14"
                     /locus_tag="ACHU9J_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR1907174.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6265..6768)
                     /locus_tag="ACHU9J_003731"
                     /pseudo
     CDS             complement(6265..6768)
                     /locus_tag="ACHU9J_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1578 a         1753 c         1771 g         1666 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcgtcg
     5701 ctatcaatcg cggaaaaagg cgtagtgcgc gaagcgaacg aagctttttg ccgtcgattg
     5761 catagctttg ttaacccttt ttccaaattt gatagcaata gttaatgttt gaagtaaagt
     5821 gttgctcaaa aacaacttcg aaggtatttg gaatactcgg gaagaaaaca tccccctctg
     5881 gctcgatgtc gatcgtcgat aagtggagcg tagaggccat gggtaatgtt tctcggtaaa
     5941 tttctccgcc accagacact ataacgtgac cggtgaattc agctagcctg tccatggcct
     6001 cttcgattga ctgaaataca actacattgt catcatttga tgtccaacct gagcgggtaa
     6061 cgaccgcgta tttcctattg gggagtgcgc ccatagattc aaacgtcttg cgacccacca
     6121 gaagccactg attgtaggtc aatgctttaa aaagtagctg ctcccctttc gcggaccagg
     6181 gtatgtctgg accgcaacca atcacgccgt ttttcgcttt cgcagctatc aatgatactt
     6241 tcaaggttct catcctgggt taacggtgcg ctcttggtcg agccgcaccg gtagctctcg
     6301 ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg gtaagaagtc
     6361 gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc gcgacagcct
     6421 gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt gctgccccat
     6481 tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta ctcccggaat
     6541 tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga cctcggggca
     6601 agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga gctcaccgcg
     6661 gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa aaacaaaatc
     6721 accagattct ccgcctctga caggcaacca gtcagaatgc gattcacc
//
LOCUS       DAZPJP010000210         6660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPJP010000210 DAZPJP010000000
VERSION     DAZPJP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.212"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHU9J_003732"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHU9J_003732"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR1907175.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHU9J_003733"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHU9J_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1907176.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHU9J_003734"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHU9J_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1907177.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHU9J_003735"
     CDS             2284..2730
                     /locus_tag="ACHU9J_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1907178.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHU9J_003736"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHU9J_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR1907179.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHU9J_003737"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHU9J_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1907180.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHU9J_003738"
     CDS             4841..5188
                     /locus_tag="ACHU9J_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1907181.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHU9J_003739"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHU9J_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1907182.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..>6660
                     /gene="dgcP"
                     /locus_tag="ACHU9J_003740"
     CDS             5647..>6660
                     /gene="dgcP"
                     /locus_tag="ACHU9J_003740"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1907183.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSA"
BASE COUNT         1616 a         1632 c         1615 g         1797 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
//
LOCUS       DAZPJP010000211         6653 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPJP010000211 DAZPJP010000000
VERSION     DAZPJP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.213"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            180..422
                     /gene="yjbE"
                     /locus_tag="ACHU9J_003741"
     CDS             180..422
                     /gene="yjbE"
                     /locus_tag="ACHU9J_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1907184.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            536..1174
                     /locus_tag="ACHU9J_003742"
     CDS             536..1174
                     /locus_tag="ACHU9J_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1907185.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1171..1908
                     /locus_tag="ACHU9J_003743"
     CDS             1171..1908
                     /locus_tag="ACHU9J_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1907186.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1908..4004
                     /locus_tag="ACHU9J_003744"
     CDS             1908..4004
                     /locus_tag="ACHU9J_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1907187.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4051..4329)
                     /locus_tag="ACHU9J_003745"
     CDS             complement(4051..4329)
                     /locus_tag="ACHU9J_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907188.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4330..4473
                     /locus_tag="ACHU9J_003746"
     CDS             4330..4473
                     /locus_tag="ACHU9J_003746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907189.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4599..5009
                     /gene="psiE"
                     /locus_tag="ACHU9J_003747"
     CDS             4599..5009
                     /gene="psiE"
                     /locus_tag="ACHU9J_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1907190.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHU9J_003748"
     CDS             complement(5053..6528)
                     /gene="xylE"
                     /locus_tag="ACHU9J_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1907191.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1657 a         1682 c         1772 g         1542 t
ORIGIN      
        1 atgattttta ttgttattta aatataagtt gaaacttata tttgatattc attccaatta
       61 tcctaaaaca ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt tgtgtatact
      121 cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg gaaattgtta
      181 tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact tctgtgtggg
      241 ctgcacctgt acaggtgggc gaagcggcag gggcggcagc aacgtcggtt tcggcgggga
      301 gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa
      361 ccggtggcgg tgatggttct aataccggga ccacaacaac cacaaccacc agtacccagt
      421 aataaagtat gtatccccaa aataattcga gtcattgcat ctgtggcttg aaatatgaag
      481 ggattaacca taaccacact ccggtgtggt tattctgccc ctctggagaa gagtcgtgaa
      541 gcgacctgca ctcattctta tctgcctgtt attacaggcc tgttcagcca cgactaaaga
      601 gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc tgacggacga
      661 tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg ggccgcaatt
      721 gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc aggatcaagc
      781 cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg acaacctgat
      841 tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg ttgatggtgc
      901 aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg ccaccgcacg
      961 ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg aaaccctggt
     1021 tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc gctactggat
     1081 cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt atttcccggt
     1141 gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt cgcgttgctt
     1201 ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt cagcaatggc
     1261 agcagtgagg ccaaaacgct aacgggcgca gagcatttaa tcgatctggt agggcaacct
     1321 cgactggcaa acagctggtg gcctggtgcg gtgattagcg aagagctggc aacggcggca
     1381 gcattgcgtc agcagcaggc gttgctgaca cggctggcag aacagggcgc agattccagc
     1441 accgacgatg ccgccgccat taacgcctta cgccagcaaa tccaggcatt ggaagtgaca
     1501 ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg cggtaacccg
     1561 ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt cacgttgttc
     1621 gggcttatca gccatcctgg caatcagcca ttcactccag gtcgcgacgt ggcgagctat
     1681 ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt cgtttacccg
     1741 gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt agagccgatg
     1801 cccggcagca ttatttatgt tggcctcgca gactccgtct ggagtgagac gcctgatgcc
     1861 cttaacgccg acattcttca gactctgacg cagcggatac cccaataatg aaaaaaagac
     1921 atctgcttag cttactggcg ctgggcatta gcacagcttg ctacggcgaa acatatcctg
     1981 cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg attattacaa actcccaccg
     2041 cgcgtatggc acgggaaggg gagttgagtc tgaactatcg cgataacgat cagtaccgtt
     2101 attactcagc ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgt tacaccgacg
     2161 tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg tataaagata
     2221 aagccttcga tctcaaactg cgtttgtggg aagagagtta ctggctgccg caagtggcgg
     2281 ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc ggaatatctt gttgccagca
     2341 aagcctgggg gccgttcgat tttacgctcg gcctgggctg gggatatctg ggcaccagcg
     2401 gtaatgtgaa aaatccgctc tgttcagcca gtgataaata ttgctatcgc gataacagct
     2461 acaaacaagc gggatctatc gacggcagtc agatgttcca cggcccggcc tcactgtttg
     2521 gcggcgtgga ataccagacg ccctggcaac cgctgcgcct gaaactggag tatgaaggta
     2581 ataattatca gcaggatttt gccgggaagc tggagcaaaa aagtaagttt aacgtcgggg
     2641 cgatttatcg cgttaccgat tgggccgacg ttaaccttag ctatgaacgt ggcaacacct
     2701 ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct gcgcccgtcc tacatcgata
     2761 acgcccgccc gcaatatcaa ccgcagccgc aggatgccat tttgcagcac tcggtggtgg
     2821 cgaatcagtt aacgttgctg aaatacaatg ccggacttgc cgatccgcag atccaggcga
     2881 aaggcgatac gctgtatgtt accggcgagc aggtgaaata tcgtgattcg cgcgaaggga
     2941 tcatccgtgc taatcggatc gtgatgaacg atctgccgga tgggatcaaa acgatccgca
     3001 ttacggaaaa tcgccttaac atgccgcagg tgacgacgga aaccgatgtc gccagcctga
     3061 aaaatcatct cgccggagag ccgttgggcc acgaaacgac gctggcgcaa aaacgcgtcg
     3121 agccagtggt tccgcagtcc accgagcagg gctggtatat cgacaaatca cgctttgatt
     3181 tccatatcga tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt tacatgtatc
     3241 agctgggcgt gatgggaacg gcagatttgt ggctgacgga ccatctgctg accatcggca
     3301 gcctgtttgc aaatcttgcc aacaactacg acaagtttaa ctacaccaat ccgccacagg
     3361 actcgcactt accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac gatgcctatg
     3421 tgaataacct gcaagccaac tacttccagc atctgggcaa cggcttctac ggtcaggtct
     3481 atggaggtta tctcgaaacc atgtttggcg gtgcgggggc tgaagtgttg tatcgtccgc
     3541 tggacagcaa ctgggcgttt ggtctggatg ccaactacgt taaacagcgc gactggcgta
     3601 gtgcaaaaga tatgatgaaa ttcaccgact acagcgtgaa aaccggacat ctgaccgcct
     3661 actggacgcc atctttcgcc caggacgtgt tagtgaaagc cagcgtcggg cagtatctgg
     3721 caggggataa aggcggcacg ctggagatcg ccaaacgctt tgatagcggc gtggtggtgg
     3781 gtggctatgc cacgatcacg aatgtttcaa aagaggagta cggcgaaggg gacttcacca
     3841 aaggcgtgta tgtctcggta ccgttggatc tcttctcgtc tggcccgaca cgcagccgtg
     3901 cggcgattgg ctggacgccg ctgacgcgtg acggtggtca gcaacttggg cgtaagttcc
     3961 agttgtatga tatgaccagc gaccgtagcg tcaatttccg ctaagtcatg ggaaaggtgc
     4021 cagttttcgc aatcactggc accctcattc ttaatggcct gttgagccat caggaacgca
     4081 gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct tcctgtccgt
     4141 cgatatcggt tgcaggtctg gttgcctgag cgactgggcc atcattgggc tggagacatt
     4201 gctttgcggt aggtcggctt tatcagcagt accgagcgga ccagcataag caggaagaac
     4261 agagactgat aacatcaaag cagcaaaata aggcttcatt tttaccacct ttatcaggtt
     4321 acgtttcatt tgttccagag gaacattgtc gatttttcgc gcattgctgg tggctgggaa
     4381 tcacctgaat gggtgatttt tgaattaccg gcttgggtgc ggtttgtctt gccggatgcg
     4441 ccgccaggcg cggcttatcc ggcctacggg taggtatatc cggctttggt ggaggcgcgc
     4501 tccaaatcca ggttgaacaa aacatacaca aaaaatatag atctccgtca catttttgcg
     4561 ttatacagga agctcgccac tgtgaaggag gtactgctat gacgtcactc tctcgtccgc
     4621 gcgtggagtt tatctccacc attttgcaga ccgtactcaa tcttggcctg ctgtgcctcg
     4681 gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca tctggcagat gtgctgttcg
     4741 cgccagaaca aaccagcaaa tatgagctgg tagaaggact ggtggtttac tttctctatt
     4801 tcgaatttat cgcgctgatt gtgaagtact ttcagtccgg ttttcacttc ccgctacgtt
     4861 actttgtcta tattgggatc accgccattg tgcgcttgat catcgtcgat cataaatcgc
     4921 cactggatgt gttgatctac tcggctgcga tcctgttact ggtgatcact ctgtggctgt
     4981 gtaattcgaa acggctgaag cgggagtaaa aagaaagcac gccgaactgg cgcggcgtgc
     5041 tggacaggaa gattacagcg tagcagtttg ttgtgttttc ttcgtttccg gttcccagag
     5101 ggcttccagc tcctcaaggg ttttaccttt ggtttccggg acaaatttcc acataaatag
     5161 tgctgccaga acgcccatac acccgtaaat ccagtaggag aaaccgttgt ggaaatgggc
     5221 caccagccag gagtttttgt ccatcatcgg gaaggtccag gagacgaagt agttcgccag
     5281 ccactgggcc gccaccgcga ttgccagcgc tttaccacga atagcattcg ggaatatttc
     5341 cgacagcagt acccagcata ccggacccca ggacatggca aaggcagcaa catagaacag
     5401 catcgacagt agcgccacaa tacccggtgc ctgagtgtaa aacgcggtac cgaggctaaa
     5461 cataccgatt gccattccga gtgcgccgat aatttgcagt ggcttacgac caaatttatc
     5521 caccgtcata attgccagaa cggtgaaggt gaggttgata actccgacaa taatggtctg
     5581 caacagcgcg atatccgtgc tggcccccag cgttttgaac acttccggcg cgtagtacag
     5641 caccacattg atgccgacaa attgctggaa gatggagagc ataacgccga tgacaatcac
     5701 gcccacgcca aacatcagca gacgaccacc ggttttgcgg ccatgatcca gggagtgttt
     5761 aatttcctgt actgcctgag ttgcaagcgt gttgcccata attttgcgca ggatactttc
     5821 cgcctgttct tgcttgccgc gtgacatcag ccagcgagga ctttctggca cggtatacag
     5881 cagcattaag aacagcagtg cagggataca ttccgaggca aacatataac gccagccgtc
     5941 agtattcagc cagctggcat caccggaacg ggcaataaaa tagtttacgc agtaaactaa
     6001 aagttgcccg aaaataatcg caaactggtt aaatgaaacc agttttccgc gaatatgagc
     6061 tggagccagt tccgcaatat acattggtga aagcattgag gctaaaccaa cgccaatacc
     6121 gccaataata cgataaataa caaattccgg gacataacct gccagataaa taggtactgt
     6181 gttgtccggg tttatagagg taaaaccaag ttctggccag gcagaaccta caccagaaat
     6241 aaaaaacagg acagcagcaa tcttaagtga atcacgacgg ccgaagcggt tactgcaata
     6301 accaccgagg gcaccgccga tgatgcaacc aatcagagcg ctggctacgc aaaatcctaa
     6361 cagggagttg gcagcggatt cacttaagtt ttgtggagca acaaagacgg tattgagtga
     6421 ctcaacagta ccggaaataa cggcggtgtc gtagccaaat aataaaccac ctaatgtagc
     6481 gactaaggta atcgaaaata tataactgga attatactgg gtattcattc agacctgcct
     6541 tagaccattc tgatctttta tcagtgcatt gatgttggaa ttgtcaggca gttaaggcaa
     6601 ttatcatttt ttacaatgcg tttacgtaat gtcttttctg tgatcttaat tgt
//
LOCUS       DAZPJP010000212         6651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPJP010000212 DAZPJP010000000
VERSION     DAZPJP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.214"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHU9J_003749"
     CDS             <1..541
                     /locus_tag="ACHU9J_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1907192.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..1139)
                     /locus_tag="ACHU9J_003750"
     CDS             complement(597..1139)
                     /locus_tag="ACHU9J_003750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1907193.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1123..1434)
                     /locus_tag="ACHU9J_003751"
     CDS             complement(1123..1434)
                     /locus_tag="ACHU9J_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1907194.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHU9J_003752"
     CDS             complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHU9J_003752"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1907195.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2521..2850)
                     /locus_tag="ACHU9J_003753"
     CDS             complement(2521..2850)
                     /locus_tag="ACHU9J_003753"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1907196.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2850..3464)
                     /locus_tag="ACHU9J_003754"
     CDS             complement(2850..3464)
                     /locus_tag="ACHU9J_003754"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1907197.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHU9J_003755"
     CDS             complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHU9J_003755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1907198.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHU9J_003756"
     CDS             complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHU9J_003756"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1907199.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1804 a         1688 c         1646 g         1513 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca
      961 ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca
     1021 aatttatcga taacagagtt accgcaatca aactgttttt gcccaggata agtaatatca
     1081 gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc
     1141 gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat
     1201 gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag
     1261 cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac
     1321 ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg
     1381 cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac
     1441 ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa
     1501 ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta
     1561 tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttgtgtt
     1621 accaaacagc agccagcagc gtatgcgagt ccggcaccat aaaatcaacg gacatcatca
     1681 ccgagagggc tgtgatggcg atgatagaga agccgaacag cttgcgcgcc cagatcctgt
     1741 catcagcaac tttataaccg cgcagggcca tacctaacca ccagacgctc accgccgcgg
     1801 cgaccaccag atatttatac ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
     1861 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
     1921 gcaataccgg aatgtttgcc gcctgataat ccttaaagcg gaaaatggcg atggcatagg
     1981 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
     2041 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
     2101 atgtgccgta gaccgagtgg cgtttcatgt acaggctata aacgccgaca taaaccacaa
     2161 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
     2221 cagcaaagcc tagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
     2281 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
     2341 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
     2401 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
     2461 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
     2521 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
     2581 gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
     2641 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
     2701 gattgttccc agaattacgg ccggagaggc agctcctgtc atcaccatcc agaacggaat
     2761 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
     2821 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
     2881 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
     2941 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
     3001 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
     3061 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
     3121 atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat gacctggctt
     3181 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
     3241 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
     3301 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
     3361 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
     3421 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
     3481 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
     3541 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
     3601 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
     3661 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
     3721 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
     3781 cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc
     3841 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
     3901 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
     3961 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
     4021 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
     4081 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
     4141 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
     4201 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
     4261 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
     4321 gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
     4381 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
     4441 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
     4501 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
     4561 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
     4621 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
     4681 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
     4741 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
     4801 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
     4861 ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag
     4921 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
     4981 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
     5041 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
     5101 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
     5161 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
     5221 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
     5281 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
     5341 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
     5401 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
     5461 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
     5521 actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
     5581 aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
     5641 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt
     5701 cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
     5761 catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc
     5821 gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag
     5881 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
     5941 gttcgccggg aaagcgattt cattcacggt tgcaatgccc tgttccgggt agatgaagaa
     6001 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
     6061 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
     6121 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
     6181 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
     6241 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
     6301 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
     6361 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
     6421 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac ggggaggtta
     6481 cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg ttacatatgt
     6541 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
     6601 gagtgcagaa ggagtttgga cgggcaattg gtataaccaa tctggaataa a
//
LOCUS       DAZPJP010000213         6611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPJP010000213 DAZPJP010000000
VERSION     DAZPJP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.215"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..233)
                     /locus_tag="ACHU9J_003757"
     CDS             complement(<1..233)
                     /locus_tag="ACHU9J_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase YliE domain
                     protein"
                     /protein_id="HFR1907200.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQ"
     gene            complement(333..1244)
                     /gene="gsiD"
                     /locus_tag="ACHU9J_003758"
     CDS             complement(333..1244)
                     /gene="gsiD"
                     /locus_tag="ACHU9J_003758"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1907201.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1247..2167)
                     /gene="gsiC"
                     /locus_tag="ACHU9J_003759"
     CDS             complement(1247..2167)
                     /gene="gsiC"
                     /locus_tag="ACHU9J_003759"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1907202.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2185..3723)
                     /gene="gsiB"
                     /locus_tag="ACHU9J_003760"
     CDS             complement(2185..3723)
                     /gene="gsiB"
                     /locus_tag="ACHU9J_003760"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1907203.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3743..5614)
                     /gene="gsiA"
                     /locus_tag="ACHU9J_003761"
     CDS             complement(3743..5614)
                     /gene="gsiA"
                     /locus_tag="ACHU9J_003761"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1907204.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5601..6566)
                     /gene="iaaA"
                     /locus_tag="ACHU9J_003762"
     CDS             complement(5601..6566)
                     /gene="iaaA"
                     /locus_tag="ACHU9J_003762"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1907205.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         1642 a         1924 c         1573 g         1472 t
ORIGIN      
        1 ttctgaataa gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga
       61 tagttaatga tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc
      121 agccttatct ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta
      181 tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc
      241 gattctacaa aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg
      301 tgattaccct ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca
      361 tcgcgtaaac catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc
      421 ggaaaaacag cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct
      481 ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag
      541 ataatcgagg tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc
      601 aagatatgac gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata
      661 aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg
      721 gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca
      781 gcgatcgcca gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc
      841 cgatcccacc agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca
      901 ccgataaaca cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg
      961 ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta
     1021 ttcagattgt cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa
     1081 atggccacca caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg
     1141 cgaaatcgtc gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc
     1201 atggcgttta acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga
     1261 tagccgggtt aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca
     1321 gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa
     1381 cgagtaagcg tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga
     1441 gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc
     1501 cgtgtttgag gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat
     1561 cttcgcttaa aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg
     1621 ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag
     1681 gcagccagcc cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg
     1741 gaaacgagat ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc
     1801 agacggcggc gataattccc gccgccatac caaatatcac cgcccagacc atactggtta
     1861 tggtcagcca cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg
     1921 acaccatcga caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt
     1981 gatacagcgg ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag
     2041 cttcgagccc ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca
     2101 ccagcaccga gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat
     2161 taagcattcc actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt
     2221 gtctggcatg atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac
     2281 caccagcggg atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg
     2341 ggtcttttcc gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt
     2401 attgctgtaa aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg
     2461 cgatagcgcc cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac
     2521 gccgctctct ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc
     2581 ggtcacctgg gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt
     2641 ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc
     2701 cgcctctttc agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg
     2761 cgcgtaggcg atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc
     2821 aactttcacc agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt
     2881 atcgaacggc ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc
     2941 catcaactcg atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa
     3001 agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg
     3061 acgccaggtt atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt
     3121 caccttcaca aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg
     3181 atgaaaacca atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc
     3241 cggatgggca agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt
     3301 tgtcggatcg atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg
     3361 attcgccgga tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt
     3421 gccatcctgg aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga
     3481 aacggtataa ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag
     3541 cccctggtaa aacgatttcg ctacggcctg agataacgtg tcattcgcgt catacggatc
     3601 gagcgtggtg aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga
     3661 tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc
     3721 cattttattc tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg
     3781 cgacgtaatg ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca
     3841 gatgaatatt gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg
     3901 gttcagcgac cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga
     3961 agaccgcgcg ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat
     4021 gactaatccg ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat
     4081 cacgctggag atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg
     4141 aaacggcttc gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga
     4201 tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca
     4261 ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac
     4321 cgtgtacacg cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag
     4381 cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg
     4441 acaaggtatc gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc
     4501 gcagcaacgc ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg
     4561 tttcgccagg ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc
     4621 gattcaacaa accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag
     4681 gttcgccatc caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa
     4741 gcgatatcaa cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa
     4801 cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg
     4861 tacccgtttc caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca
     4921 ccacgcccat atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt
     4981 tgattaattg caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg
     5041 caatcagcac cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc
     5101 cgccagagag ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct
     5161 gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac
     5221 gaattgattc ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct
     5281 cctgaaatat catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct
     5341 cgctaagttc aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta
     5401 aaccgcccgc ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc
     5461 cggattcgcc aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga
     5521 ccgcagctat tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca
     5581 cattaccggc atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata
     5641 ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac
     5701 ggtagcgcga cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca
     5761 gggagttttt ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc
     5821 attaacgcgg cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata
     5881 caagaaaccg ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg
     5941 ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg
     6001 ccgtctaaat ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc
     6061 gcaccgctat ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa
     6121 cgcaaaggcg tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa
     6181 ttttctgccc cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg
     6241 gcaagaaccg gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta
     6301 ccatccatca cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt
     6361 ccggcgttaa acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc
     6421 gcactttcgc ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca
     6481 atgtagcgta attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca
     6541 tgaattgcaa tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt
     6601 gctataaata t
//
LOCUS       DAZPJP010000214         6598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPJP010000214 DAZPJP010000000
VERSION     DAZPJP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.216"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..969)
                     /gene="rfaC"
                     /locus_tag="ACHU9J_003763"
     CDS             complement(<1..969)
                     /gene="rfaC"
                     /locus_tag="ACHU9J_003763"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1907206.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(973..2019)
                     /gene="rfaF"
                     /locus_tag="ACHU9J_003764"
     CDS             complement(973..2019)
                     /gene="rfaF"
                     /locus_tag="ACHU9J_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1907207.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2029..2961)
                     /gene="rfaD"
                     /locus_tag="ACHU9J_003765"
     CDS             complement(2029..2961)
                     /gene="rfaD"
                     /locus_tag="ACHU9J_003765"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1907208.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3175..4371
                     /gene="kbl"
                     /locus_tag="ACHU9J_003766"
     CDS             3175..4371
                     /gene="kbl"
                     /locus_tag="ACHU9J_003766"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1907209.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4381..5406
                     /gene="tdh"
                     /locus_tag="ACHU9J_003767"
     CDS             4381..5406
                     /gene="tdh"
                     /locus_tag="ACHU9J_003767"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1907210.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            5645..6547
                     /locus_tag="ACHU9J_003768"
     CDS             5645..6547
                     /locus_tag="ACHU9J_003768"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1907211.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         1569 a         1645 c         1706 g         1678 t
ORIGIN      
        1 aatcatagca gcgttttctt caatgaatcg cttaacagca tttgctgtca attgagacaa
       61 ctcattcccc ggagccctac aaaccatctg attcttccca tatccaccaa ttaatcccgg
      121 atcggttggt ccataaaccg tgatattggg tctatccagt gccgccgtta aatggcttaa
      181 ccccgtatcc accgacacta caaatttagc cccggccagc acgcgggcaa cgccttccag
      241 actcatcttc ggcaatactt caacataagc aaatccttcc gccagtcgtt tcgcccgctc
      301 ttcctcatgc ggcgcgcccc acggaagttt aatccgtatt cctgaatcag ccagtaaacc
      361 aatcaattct cgccagtgtt cttccggcca gtgtttatca tcacgggtcg tcgcatgaag
      421 aaatacggca tattcgccag catctgtagg caggttcgtc aggaaatgct gtgcaatagc
      481 ataatcgccc tgggtttgcg gtttgctata gcccagactt ttggcaaaca gttcgcgggt
      541 gcgttctacg gcgtgctgct gttttgcaat atgatgctta cgattgtaaa acagactggc
      601 taaaggttcg cgagcggttt gccagtccat gccatgcttt acgccatgcg ccagacgcgt
      661 caccagcgcc gcgcttttta ccagcccctg agcgtcgata actgcgtcat agttctctgc
      721 ttgtagcgct tcacgaaacg ctttgcgttc cgcttttatg ggggccgaga accaggcttt
      781 acgccagcga cgtattgcca caggaataac tcgctcaacg gcggcgtgcc aggaaggaat
      841 ctgtgcgaac ccttcttcca ccacccagtc aaacttaatc cctgggattg cctgctgggc
      901 atcagtgagt gcgggcaacg tatggagaac atcgcccatc gacgatgttt taacgatcaa
      961 aacccgcatc cgtcaggctt cctcttgtaa caatagcgcg ttgagttctt ccagtacgcg
     1021 ctggggagta atgtcgatta agctctggtg ataaccctcc gcagcgtcac ctttacgcac
     1081 tttgtgatag ccggtaatca ggcggatcac gcgcgcttta tgggatagcg gcggtgtgaa
     1141 gtccgggcta ctcggaccat acagggcaac cagcggacga ttgagcgccg ccgcaacgtg
     1201 catcaggcca gaatcgttag tgacaatggc tttacaggct gcaatcagga taaccgcttg
     1261 atcaagctgt gtttcccccg ccaggttccg acaccatgcc tgctgctcgg tattcaaagc
     1321 ggcaagaatc tcattgcccg cttcatgatc tttcgccgag ccaaacagaa ccacctgata
     1381 accttcatca atcagctgct ttgccagttc cgcatagtgg tagtgtggcc agcgttttgc
     1441 cggaccaaac tccgcacccg ggcaaaaacc aatcatcgga cgttctgatg aaagcgaaaa
     1501 ttgattacag gtatatgatt tttcaccttc gctcacctgc aattgcggcc ataacaacgg
     1561 ttttggtaga tcttgtgcgg tacgcatcac gcctttgtca taggccagcg cgacatagcg
     1621 ttccaccatt agcggccagg cttctttatc gagcacgcgt aaatcgttga gtaaaccgta
     1681 gcgcatctcg ccgcgccagc cggtgcgatg aggaataccc gcgaagaaag gcactaatgc
     1741 agatttgaag gagttgggta agacgtaggc gcggtcgtag cgcttttcac gcaggctatg
     1801 acccagtttg cggcgttcgc cgatttccag cgctccgtga ccgagaggca taggaatagc
     1861 ttcgttaact tccggcatcc gcgataataa tggacggcac catgccggtg ccatcacatc
     1921 gattatcgcc tggggatagc gcgcctggag cgtgcgatag agactttgcg acatcatcat
     1981 gtcgccaacc caagacgggc cgatcaccag tattttcatg cagagctctt atgcgtcgcg
     2041 attcagccag gccatgtatt ccgttacacc ttcagcaacg gttttgaacg gtttgtcgta
     2101 acccgccgcg cgcagatttg tcagatctgc ctgagtgaac gcctggtagc ggcctttcag
     2161 cttatccggg aacggaatgt attcgatctg gcctttcttg tgataagcca gcgtagcatc
     2221 agctacagcc tggaaggatt ccgcacgacc agtaccgagg ttgaagatgc cggaaacgcc
     2281 attttccagg aaccacagat tcacatcagc cacgtcgccc acatagacga agtcgcgttt
     2341 gaagttctcg ctaccttcaa acagcttcgg tgattcaccg ttgttaagct gggtgttgag
     2401 atggaaagcg acgctcgcca tgctgccttt atggccttca cgcggtccat aaacgttgaa
     2461 atagcggaag ccaacaatct gcgagttagc ttcaggcagg atttggcgaa catattcatc
     2521 aaacaggaat tttgagtaac cgtagacgtt caacggtttt tcgtactcgc gggattcaat
     2581 aaagtcggag gtgcgtccgc cgtaggtggc tgcggaagag gcatacagga acgggatttc
     2641 gcgctccaga cagtagtgca gcagctcttt ggagtattga tagttgttat ccatcatata
     2701 cttgccatcc cactcggtgg tggaagagca cgcgccttcg tggaaaatcg cttcgacatc
     2761 gccgaactct tcgccagcca taatctggat caggaagtct tccttatcca tatagtccgc
     2821 gatatccaga tccaccaggt tcacaaactt ggtgccgtct ttcaggttgt ccaccaccag
     2881 aatatcggtg atgcctttat cattcagggc cttaacgatg ttgctgccga taaagcccgc
     2941 gccgccggta acgatgatca taactgtaac cttcgaatta tggagtcaga gacaatctca
     3001 gacacgaata cttctatcat atcactacac ggcgttggct tcagccattc gccaaaagtg
     3061 agattgatgg acgttattta tcctgaattt tgcagaagtg ttaacgcgtc atctcgtcgc
     3121 gacctataag tttgggtaat atgtgctgga atttgccctg tctggagaat cgcaatgcgt
     3181 ggagaatttt atcagcagtt aaccaacgat ctggaaaccg cacgggcgga agggttgttt
     3241 aaagaagagc gcattattac gtctgcgcag caagcagata tcactgtggc tgatggaagc
     3301 cacgtcatta acttttgtgc caataactat ctcgggctgg cgaattatcc ggatctgatt
     3361 gcggcggcaa aggcgggaat ggattctcac ggtttcggca tggcttcggt gcgttttatt
     3421 tgcggcactc aggacagcca taaagagctt gaacaaaaac tggcggcatt cctggggatg
     3481 gaagatgcga ttctctactc ttcctgcttt gatgctaacg gtggcctgtt tgaaacgctg
     3541 ctgggcgcgg aagacgccat tatctccgac gcactgaacc acgcgtctat tattgatggt
     3601 gtgcgtctgt gtaaagctaa acgctatcgc tatgccaaca acgatatgca ggagctggaa
     3661 gcacgtctga aagaagcgcg tgaagccggt gcgcgtcatg tgctgattgc caccgatggt
     3721 gtgttctcga tggacggcgt gattgccaac ctgaagggcg tttgcgatct cgcagataaa
     3781 tatgatgccc tggtgatggt agacgactcc cacgcggtcg gttttgtcgg tgaaaatggt
     3841 cgtggttccc atgaatactg cgatgtgatg ggccgggtcg atattatcac cggcacgctc
     3901 ggtaaagcgc tgggcggggc ttctggtggt tataccgcgg cgcgcaaaga agtggttgag
     3961 tggctgcgcc agcgttctcg tccgtacctg ttttccaact cgctggcacc ggccattgtt
     4021 gccgcatcca tcaaagtact ggagatggtc gaagcgggta gcgagctgcg tgaccgtctg
     4081 tgggcgaacg cgcgtcagtt ccgtgagcaa atgtcggcgg cgggctttac cctggcggga
     4141 gccgatcacg ccattattcc ggtcatgctt ggtgatgcgg tagtggcgca gaaatttgcc
     4201 cgtgagctgc aaaaagaggg catttacgtc accggtttct tctatccggt cgttccgaaa
     4261 ggtcaggcgc gtattcgtac ccagatgtct gcggcgcata cccctgagca aattacgcgt
     4321 gcagtagaag cattcacgcg tattggtaaa caactgggcg ttattgcctg aggatgtgag
     4381 atgaaagcgt tatccaaact gaaagcggaa gagggcatct ggatgaccga cgttcctgta
     4441 ccggaactcg ggcataacga tctgctgatt aaaatccgta aaacagccat ctgcgggact
     4501 gacgttcaca tctataactg ggatgagtgg tcgcaaaaaa ccatcccggt gccgatggtc
     4561 gtgggccatg aatatgtcgg tgaagtggta ggtattggtc aggaagtgaa gggcttcaag
     4621 attggcgatc gcgtttctgg cgaaggccat atcacctgcg gtcactgtcg caactgtcgt
     4681 ggcggtcgta cccatctgtg ccgcaacacg atcggcgtcg gcgttaaccg cccgggctgc
     4741 ttcgccgaat atctggtgat tccggcgttc aacgccttca aaattcccga caatatctcc
     4801 gacgacctgg cttccatttt tgatcccttc ggtaacgccg tgcataccgc gctgtcgttc
     4861 gatctggttg gcgaggatgt gctggtctct ggtgcaggcc cgattggtat tatggcagcg
     4921 gcggtggcga aacacgttgg tgcacgcaat gtggtgatca ctgatgttaa cgaatatcgt
     4981 ctcgaactgg cgcgcaaaat gggtatcacc cgtgcggtca acgtcgctaa agaaaacctt
     5041 aatgatgtga tggcagaact gggcatgacc gaaggctttg atgtcggtct ggaaatgtcc
     5101 ggtgcgccgc cagcgtttcg taccatgctc gacaccatga accacggcgg tcgcattgcg
     5161 atgctgggta ttccaccgtc cgatatgtct atcgactgga ccaaagtaat ctttaaaggc
     5221 ttgttcatta aaggtattta cggtcgtgag atgtttgaaa cctggtacaa gatggcggcg
     5281 ctgattcagt caggtctgga tctctcgccg atcattaccc atcgtttctc tatcgatgat
     5341 ttccagaagg gctttgacgc tatgcgttcg ggccagtccg ggaaagtaat tctgagctgg
     5401 gattaacacg aacaagggct ggtattccag cccttttgcc tgaggataat ccgttaaata
     5461 tgtaaaatcc tgtcagtgta ataaagatat cgtaattgcg ctgatctctt atatagctgc
     5521 tctcattatc tctctaccct gaagtgactc tctcacctgt aaaaataata tctcacaggc
     5581 ttaatagttt cttaatacaa agcctgtaaa acgtcaggat aacttcagag gtcgtcggta
     5641 atttatgatg aacagcacca ataaacttag tgttattatt ccgttatata atgcgggcga
     5701 tgatttccgc acttgtatgg aatctttaat tacgcaaacc tggactgctc tggaaatcat
     5761 tattattaac gatggttcaa cggataattc tgttgaaata gcaaagcatt acgcagaaaa
     5821 ctatccgcac gttcgtttgt tgcatcaggc gaatgctggc gcatcggtgg cgcgtaatcg
     5881 tgggattgaa gtggcaacgg gcaaatatgt cgcttttgtc gatgctgacg atgaagtcta
     5941 tcccaccatg tacgaaacgc tgatgaccat ggcgttagag gacgacctcg acgtggcgca
     6001 gtgcaacgct gactggtgtt ttcgtgaaac gggagaaacc tggaaatcca tccccagcga
     6061 tcgccttcgc tcaaccggcg tattaaccgg cccggactgg ctgcggatgg ggctttcttc
     6121 gcgccgttgg actcacgttg tctggatggg ggtttatcgc cgcgatgtta ttgttaaaaa
     6181 taacattaaa tttattgccg gattacatca tcaggatatt gtctggacaa cagaatttat
     6241 gtttaatgcg ctgcgtgcgc gatataccga gcaatcgtta tataaatatt atctgcataa
     6301 tacgtcagtg agtcggttgc atagacaagg gaacaaaaac cttaattatc aacgtcacta
     6361 tattaagatt actcgtctgc tagagaaatt aaatcgaaat tatgccgaca aaattacgat
     6421 ttatccggaa tttcatcagc aaataactta cgaagcattg cgtgtttgcc atgcggtgcg
     6481 caaagagccg gatattatta cccgccaacg gatgattgcc gagatattta cttccggtat
     6541 gtggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactt
//
LOCUS       DAZPJP010000215         6597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPJP010000215 DAZPJP010000000
VERSION     DAZPJP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.217"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            260..607
                     /locus_tag="ACHU9J_003769"
                     /pseudo
     CDS             260..607
                     /locus_tag="ACHU9J_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            660..1055
                     /locus_tag="ACHU9J_003770"
     CDS             660..1055
                     /locus_tag="ACHU9J_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1907212.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1096..1839
                     /gene="cmoA"
                     /locus_tag="ACHU9J_003771"
     CDS             1096..1839
                     /gene="cmoA"
                     /locus_tag="ACHU9J_003771"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1907213.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1836..2807
                     /gene="cmoB"
                     /locus_tag="ACHU9J_003772"
     CDS             1836..2807
                     /gene="cmoB"
                     /locus_tag="ACHU9J_003772"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1907214.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2972..5401)
                     /gene="torZ"
                     /locus_tag="ACHU9J_003773"
     CDS             complement(2972..5401)
                     /gene="torZ"
                     /locus_tag="ACHU9J_003773"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1907215.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5426..6526)
                     /locus_tag="ACHU9J_003774"
     CDS             complement(5426..6526)
                     /locus_tag="ACHU9J_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1907216.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1516 a         1705 c         1565 g         1811 t
ORIGIN      
        1 cctgaaaaga gacttcacag attctttata tatttcaata agttatgcta aaaagagact
       61 ataccgatac tgcatagaat ttgtgtagga ttgcatagat acttggaaaa acaccgttaa
      121 attgcatagt cattcacctg atattgtcct gtttttacag gtagttgatt cggatgttct
      181 aggaatcatc ctcatagttg attcggttag ttgggcttgt accaacccct aatcaacctc
      241 atagtgtatt tagaccttga tgattttaga cagccgccct gttcatgcag cacgtccaca
      301 cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa gttccggtac atgctgtact
      361 gacggcgaca aatccgctga ttcgttttat cggtagtgat gatatgacgc aaaaccggga
      421 attatttcag gtctggttac agaaattagc gcagtggcat cagaccacta cgccttatct
      481 ttttttacat acgccagaca ttgcccaggc cccggaactg gtacataccc tgtgggaaga
      541 cttacgtaaa acgcttccag agatcggagc agttccggct attccacagc aatcttctct
      601 tttctgaatt tgccacctat catagacagg tgccatcggc cattttaaag ggagtttgta
      661 tggtaagcgc gctatatgcc gttttaagtg cgttgttatt aatgaagttc tcttttgatg
      721 tcgttcgcct gcgaatgcag taccgcgttg cctatggtga cggtggtttt agcgaactgc
      781 aaagcgctat tcgcattcat ggtaacgcgg tggaatatat tcccatcgcg attgtgctga
      841 tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt gcatatttgc ggcatcgttt
      901 tgcttgctgg tcgtctgatg cattattacg gttttcatca ccgtctgttt cgctggcgac
      961 gttccggcat gagcgccacc tggtgtgcgc tgttgctgat ggtgctggcg aatctttggt
     1021 atatgccctg ggagttggtt ttctccctgc gttagcgcac aatacgccac tttctttttc
     1081 ccggattttt acgttatgtc tcaccgcgac acgctatttt ctgcccctat cgccagactg
     1141 ggcgactgga cctttgatga acgggtagct gaagtcttcc cggatatgat ccagcgttcc
     1201 gttcccggct attccaatat tatttccatg attggtatgt tagccgagcg cttcgttcaa
     1261 cctggtacgc aggtttacga tctgggttgt tctctgggcg cggcgacgct ctcggtgcgt
     1321 cgcaacattc atcatgataa ttgcaaaatt attgccatcg acaactcccc ggcgatgatt
     1381 gaacgctgca gtcgtcatat tgacgcctat aaagccccta cgccagtaga cgtcattgaa
     1441 ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg tggtgctgaa ttttaccctg
     1501 caattcctgg aaccttccga gcgccaggca ttactggata aaatttatca aggactgaac
     1561 ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg aagatgccaa agttggtgaa
     1621 ctgttgttca acatgcacca cgactttaaa cgtgccaacg gttacagcga actggagatc
     1681 agccagaagc gcagcatgct ggaaaacgtg atgctgaccg attccgtgga aacccataaa
     1741 gcacgcctgc ataaagccgg ttttgagcat agcgagctgt ggttccagtg ctttaacttt
     1801 ggttcactgg tggcattaaa agcagaggac gctgcatgat cgactttggt aacttttatt
     1861 ctctgattgc caaaaatcat ctttcacact ggctcgaaac gctgcccgcg cagattgcta
     1921 actggcagcg cgagcagcag cacgggctgt ttaagcagtg gtccaacgcg gtggaatttc
     1981 tgcctgaaat taaaccgtat cgtctggatt tattgcatag cgtaaccgcc gaaagcgaag
     2041 agccactgag caccgggcaa attaaacgca ttgaaacgct tatgcggaac ctgatgccat
     2101 ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga taccgaatgg cgttccgact
     2161 ggaaatggga tcgtgttctg ccccatcttt ctgatttaac cggacgcacc attctggatg
     2221 tcggctgcgg cagcggttat cacatgtggc gcatgattgg cgcaggcgcg cacctcgcgg
     2281 tggggatcga ccctacgcaa ctgtttctgt gccagtttga agcggtgcgt aaactactgg
     2341 gtaacgatca gcgcgcacat ttgttaccgt taggtattga acagcttccg gcactgaaag
     2401 cctttgatac cgtcttttcg atgggcgtgc tttaccatcg ccgttcgccg ctggagcatc
     2461 tctggcagtt gaaagatcaa ctggtgaatg agggtgaact ggtgctggaa acgctggtta
     2521 ttgatggcga tgagaacacg gtgctggtac caggcgatcg ttacgcgcaa atgcgtaatg
     2581 tctatttcat cccttccgcg ctggcgctga aaaactggct gaagaagtgt ggttttgtag
     2641 atatccgcat tgtggatgtg tgcgttacca ctactgaaga gcagcgacgc actgaatgga
     2701 tggttaccga atctctgtcc gatttcctcg acccacatga tccgagtaag acagttgaag
     2761 gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa gccgtaaagg tctggtatta
     2821 ctgccggatg cggcgtgaat gccttatccg gcctacaaac ccttgctaat tcaatctatt
     2881 gcagggacta tgtaggcctg ataagcatag cgcatcaggc agttttacgt ttgcataacc
     2941 tcagcgcccg tttccgggcg ctattcactt cttacgcctg aacagcaggc ttatcaaatg
     3001 ccgttaactt cggtgcgtta cccgtatatt tttcgatata caccagcgca gagttaccgg
     3061 cacaggcatt tgccagctgc gagctgggga tatccgccgt taacacgttc gcactgccgt
     3121 ttttacacaa gccgttttcc agatctggcc atgcaccttc atgcacgcat accacgcctt
     3181 ttttgatccc gtcagtcacc accgcgcctg tcaaaatctg accacgtttg ttccagacac
     3241 gcaccagatc gccattcgca ataccaaatc gagcagcatc ttcggtgtga atagtgatcg
     3301 gttcacgatc ggcaaccgca tattttttac gcagttccgc atagttaagc tgactgtgta
     3361 aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt ctcgtcggcg gtacccttcc
     3421 actcatcagg cgcaagccag gttgggtgtg ccgggcaatc cttatagcca aatttttcca
     3481 gcgttttgga gtaaatctca attttgccac ttggcgtacc cagcgcattt ttcaccggat
     3541 cggcgcggaa atcaccataa cgaacgtact gttcgttctt ctcgctgcgg cgcatttcga
     3601 tcagtttatt ttgctgccag aaggcattaa acattggcat agtgacgcgt tgcgcacggg
     3661 cccctttctg agcggcatca tagaaaaatt tcagccacgc catttcatct ttaccttcgg
     3721 tatagatctc ttttccgcca ggtttgagta attccgcaag atcggcaaac acatcaaaat
     3781 cgtttcgcgc ttcaaattgc ggagcaacag cctgcttcat cggcacaata tgctggttgc
     3841 tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga tgtggtgatc ggtaatacga
     3901 tatctgcgtg tttagcagct gcggtccagt agcattcaga aacgacgatc atctccggtt
     3961 tctgccatgc tttaatcaga cgattagtat cctggtgatg ggtaaagtta ccgccacccg
     4021 cccaccagat cattttgata ttcggatacg tttgctcttt accgttatgc tgatatttgc
     4081 cgccaggatt ttccagcgca tcgacgatac gggcaacagg aatcgccctc attccgccat
     4141 catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga catttccggc agcacgccgc
     4201 caacacgtgt cgggttgcca ccattggagt aatgataaga gaaaccaaaa ccgccgcccg
     4261 gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag catccagtgt ttctgttcac
     4321 catattgctg gcgctgaatt ccccagcctg ccatcagcat tgtgcgattc gccgccatca
     4381 gttcagcgag tttgactatc tgcgcttccg gtacgccagt aatttccgcc gcccaggccg
     4441 cgctcttcgg cgtattatcg ctcttacctc tcagatactc ttcaaactgc ggatacccgg
     4501 tagtgtattt ttcaaggaac actttatcgt gtttgccttg tgtcatcagg gtatgcgcaa
     4561 tccctaacat cagtgccacg tcggtgccca tattcggcgc gatccaggtg gcattatcgc
     4621 caaagaagtc gatggtttcg gagcggatag gatcaatggc aatcactggt ttgccagatt
     4681 ttttcagctg atggaagtat tccagccctt gctcatcggt actgctccag gcaattttta
     4741 aggtattcag cgggttcatc ccccacagca ccacaacctg gctgttttcc agaatcagcg
     4801 gccaggaggt ctgctgttca tacacctcta cagaaccgac cacatgcggc atgatcacct
     4861 gtgccgcccc ggttgaataa tcgccgctat gcccggaata accgcccgcc aggttcatat
     4921 aacgttgcag taaggtttgc gctttatgca acacgccaga agagcgccag ccgtatgatc
     4981 cggcaaaaat agccgatggt ccgttagctt tgcgaatgcg atcgtgttgc tcatgaatca
     5041 gttttaatgc ttgttcccag ctcacctgta cataggtatc ttcgccacga cctttcgtcg
     5101 gttgcagtgg attatcgaga tagctttttc tcaccatcgg atgctgaata cgcgtcgtgg
     5161 tgtgtacctg atccgccgcc gtagactgta aggaattcgg tatggttttc gccagcgcgc
     5221 ctgttgaaga aacaatcttg ccgtccttca cttctacgtt catcgctccc caacgtcccg
     5281 cggtgaggat tttaccgtcc ttctcttctg cccatgcggg gagcggtgct gccgatgtca
     5341 ccaccagcgc tccagcggca ataccgctgt gtttaataaa ttcacgtctt gttaatgtca
     5401 taacttcctc cctgatcaac gaggatcact gtttctcggt aatatctttg gcgttgtact
     5461 ggaaataccg cgttaaaatg tccagttcgt tttcgctcat gctggttcgt gcccccattc
     5521 ctttggcaat ggacggccac gcattgacgg tgtaatggtc ggcggcaata ggggcatgac
     5581 aaccagcgca ataggtatcg tcaagttttt cagcgtattg ccatagcggt ttacggtccg
     5641 ctaatccggg atcggtaagc gcaccctgta aagacgcctg acgccattga ttgccgtatt
     5701 cgtcagcctg ccattccccg tttaccgtga gcgccttgat accttcttca cttaatgtgg
     5761 ctagcgccag ccgttgacct gccgccaggt agagcgtgtt ttcactgccc tgcatttgat
     5821 aaccctgcaa cagaacgatc ggctgtttgc cactggcatc aacgacggtg agatcggtac
     5881 caggattcac ggtagccagc tcgcctatgc gagaagtttt gaaaggataa atatgtgcgc
     5941 cattagtcac tgaagtagcg gcctgacttt ccagctcatg cgccgcgttg tcatccattt
     6001 ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc gatacaggtt tcgccgccct
     6061 tttgtgcttt gttatgcatt ttctgcgcag attcactttg cgaggcaata tccatggcat
     6121 caaaagaatg gcaactacgg cacgttgcag agtcagttgc ttttaattct ttccatactg
     6181 tttcggccat ttcctggcga tgagtttcga acttatcgtc actgtctatt ttgccgctaa
     6241 caaattcatg ataaatatct ttagatgctt ttaatttagc aaataaataa tccatccctg
     6301 actttggaat atggcaatct gcacattccg cacgtatccc tttctggttc gaaaagtgga
     6361 cagttccctg atattcctca aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg
     6421 tatccgacgt tttatgtaag gctttttgcg ccagcaataa cccgccgcca ccaaccacaa
     6481 ccgctatcag caaaaacaat aacccaatgc gttttttccc tcgcatagat tcctctaaat
     6541 aaattccaga acaccgaaat cattgtataa ttaaatacac aacgaattaa ccgcttg
//
LOCUS       DAZPJP010000216         6584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPJP010000216 DAZPJP010000000
VERSION     DAZPJP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.218"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            135..821
                     /locus_tag="ACHU9J_003775"
     CDS             135..821
                     /locus_tag="ACHU9J_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1907217.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(912..1048)
                     /locus_tag="ACHU9J_003776"
     ncRNA           complement(912..1048)
                     /ncRNA_class="other"
                     /locus_tag="ACHU9J_003776"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1093..1180)
                     /locus_tag="ACHU9J_003777"
     tRNA            complement(1093..1180)
                     /locus_tag="ACHU9J_003777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1144..1146),aa:Ser,seq:gga)
     gene            1414..2352
                     /gene="ghrA"
                     /locus_tag="ACHU9J_003778"
     CDS             1414..2352
                     /gene="ghrA"
                     /locus_tag="ACHU9J_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1907218.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2407..3144
                     /gene="ycdX"
                     /locus_tag="ACHU9J_003779"
     CDS             2407..3144
                     /gene="ycdX"
                     /locus_tag="ACHU9J_003779"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1907219.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3168..3722
                     /gene="ycdY"
                     /locus_tag="ACHU9J_003780"
     CDS             3168..3722
                     /gene="ycdY"
                     /locus_tag="ACHU9J_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1907220.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3824..4315
                     /locus_tag="ACHU9J_003781"
     CDS             3824..4315
                     /locus_tag="ACHU9J_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1907221.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4380..5213)
                     /gene="csgG"
                     /locus_tag="ACHU9J_003782"
     CDS             complement(4380..5213)
                     /gene="csgG"
                     /locus_tag="ACHU9J_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR1907222.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPQAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5240..5656)
                     /gene="csgF"
                     /locus_tag="ACHU9J_003783"
     CDS             complement(5240..5656)
                     /gene="csgF"
                     /locus_tag="ACHU9J_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1907223.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5681..6070)
                     /gene="csgE"
                     /locus_tag="ACHU9J_003784"
     CDS             complement(5681..6070)
                     /gene="csgE"
                     /locus_tag="ACHU9J_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1907224.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6075..>6584)
                     /gene="csgD"
                     /locus_tag="ACHU9J_003785"
     CDS             complement(6075..>6584)
                     /gene="csgD"
                     /locus_tag="ACHU9J_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1907225.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         1652 a         1505 c         1631 g         1796 t
ORIGIN      
        1 taaataggga tggttgtaat aatcaaaaca tgatactaac tatatattga tcgttgcatt
       61 gttggcatgt aacacgctgc agcgagtaac attggtaatg aaatataatc aacatggata
      121 ggtgaaaatt aattatgagc tttatggctt ttgtaacttg tttgacaatt gcatttatcg
      181 ccggttttgc tgttgccaga aaattgacac tccgaaaagt tgcctcatcg gtaatcatcg
      241 ctaaatcgca acccgtccct tcatctgcaa cttcccctgc cgctaaggcg tcgacaagga
      301 gctatatacc gcagaaaacc tatcgtgact ctcgttggca agagcgagat gagttatttc
      361 agggaatggt tgccgaaggt attttattgg aaagaaaaga gtggttaatc acgcgttgtg
      421 aaaaaatgta tttcgataat ctggaaaaat ggtttggaca ttattgtcat atccattgcc
      481 aggtctctct ggggcgcttg atcaactttc cagaacaacc tgagtttagc gaagaagaaa
      541 gaaagcgttt ctttgcaatt tataatggca tggcaatgga ttatgttctg gtttcaaaga
      601 agaacaataa aatagtctgt gttattgagc tggatgaccc gagtcatgaa agggcggatc
      661 gcattattcg tgaccgttgg ttggatacaa ttatgactct tgcacgggtg ccttttgtac
      721 gagcttctgt cactaaattg gaggaaattc ctccggtctg ggagactcgt gacaaaattc
      781 gcccattagt cgcggcgaca aagagtgaac cggtgcatta actaagtatc ccggtcgggg
      841 cttctcatcc ccccggtgtg tgcaatatac gaaaaaaagc ccgtaccttc gtacgagctc
      901 ttctttaaag atggcggtga gggggggatt gactcgcttc gctcgccctg cgggcagccc
      961 gctcactgcg ttcacggtct gtccaactgg ctgtcgccag ttgtcgaacc ccggtcgggg
     1021 cttctcatcc ccccggtgtg tgcaatatac gaaaaaaaag cccgtacttt cgtacgagct
     1081 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
     1141 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtcgt tcctgtcttg
     1201 ctggaacggg cgctaattta gggaaatcat gacctgaggt caacaaactt tttgaaaaaa
     1261 tcgcgcgttt attcaaactt caatcaatgt gtggttttaa taagcgaaat ctgctttttt
     1321 tgccaccgac cacggatttg ttatgctggt ggcctttgta gatcataacg ataagtgcga
     1381 ataaatttcg cacaacgctt ttcgggagtc agtatggata tcatctttta tcacccaacg
     1441 ttcgataccc aatggtggat tgaggcactg cgcaaagcta ttcctcaggc aagagtcaga
     1501 gcatggaaaa gcggagataa tgactctgct gattatgctt tagtctggca tcctcctgtt
     1561 gaaatgctgg cagggcgcga tcttaaagcg gtgttcgcac tcggggccgg tgttgattct
     1621 attttgagca agctacaggc acaccctgaa atgctgaacc cttctgttcc actttttcgc
     1681 ctggaagata ccggtatggg cgagcaaatg caggaatatg ctgtcagtca ggtgctgcat
     1741 tggtttcgac gttttgacga ttatcgcatc cagcaaaata gttcgcattg gcaaccgctg
     1801 cctgaatatc atcgggaaga ttttaccatc ggcattttgg gcgcaggcgt actgggcagt
     1861 aaagttgctc agagtctgca aacctggcgc tttccgctgc gttgctggag tcgaacccgt
     1921 aaatcgtggc ctggcgtgca aagctttgcc ggacgggaag aactgtctgc atttctgagc
     1981 caatgtcggg tattgattaa tttgttaccg aatacccctg aaaccgtcgg cattattaat
     2041 caacaattac tcgaaaaatt accggatggc gcgtatctcc tcaacctggc gcgtggtgtt
     2101 catgttgtgg aagatgacct gctcgcggcg ctggatagcg gcaaagttaa aggcgcaatg
     2161 ttggatgttt ttaatcgtga acccttaccg cctgaaagtc cgctctggca acatccacgc
     2221 gtgacgataa caccacatgt cgccgcgatt acccgtcccg ctgaagctgt ggagtacatt
     2281 tctcgcacca ttgcccagct cgaaaaaggg gagagggtct gcgggcaagt cgaccgcgca
     2341 cgcggctact aataaagcat caggattcct gctatccttg gcgggaattg aatacaggag
     2401 agagttatgt atcccgtcga ccttcatatg cataccgttg ccagcacaca tgcatatagc
     2461 acattgagtg attacattgc ccaggccaaa caaaagggca ttaaactttt tgcgatcacc
     2521 gatcatggcc cggatatgga agatgctccg catcactggc acttcattaa catgcgtatc
     2581 tggccgcgag tggttgatgg ggtagggatc ctgcgcggca tcgaagctaa cattaaaaat
     2641 gttgatggtg aaattgattg cagcggtaaa atgtttgact cgctggatct gattattgcc
     2701 ggttttcatg agccggtttt tgcaccacat gacaaagcga ccaatacaca agcgatgatc
     2761 gccactatcg ccagcggcaa tgtgcatata ataagccatc ccggaaatcc caaatatgaa
     2821 atagatgtga aagccgttgc tgaagcagcc gcgaaacatc aggtggcgct ggaaatcaat
     2881 aattcttcat ttttacactc acgtaagggc agtgaagaca actgtcgtgc ggtagccgca
     2941 gcggtacgtg atgctggagg ttgggtggca ttaggctcgg attctcacac cgcgtttacc
     3001 atgggggaat ttgaagagtg tcttaaaatc ctcgacgcgg tagattttcc gccagagcgc
     3061 attttgaatg tttctccgcg ccgcttactg aacttccttg aatctcgcgg tatggcaccg
     3121 attgcggaat ttgcagacct ttaattacta gtaatggaaa tatattaatg aacgagtttt
     3181 ctatcctctg tcgtgtgctg ggttcgctct attaccgcca accgcaagat cctttactgg
     3241 tgccgctgtt taccctgatt cgtgagggga aactggctgc gaactggcca ctggagcagg
     3301 atgagttact gacgcgttta cagaaaagct gtgatatggc gcaagtctct gccgattaca
     3361 atgcgttgtt tatcggcgat gaatgtgcag tgccgccata tcgtagcgca tgggttgagg
     3421 acgtaacgga agcggaagtg cgcgcttttc tttccgaacg agggatgcca ctggcggata
     3481 cgccagccga tcacatcggc acattgctgc ttgcggcttc ctggctggaa gatcagtcaa
     3541 cggaagatga gagcgaagca ctggaaacac tgttcagtga gtatctgtta ccctggtgtg
     3601 gtgcgttcct cggcaaagtg gaggcccatg caaccacgcc tttctggcgc accatggcac
     3661 cgctaacccg tgatgccatt agtgcaatgt gggacgagct ggaagaagat tctgaagagt
     3721 aaatgtgatc atcgtcactc ataactgcaa cagataattt gttgttgcat aaaatgtgtg
     3781 ctcgatctca ttcatggccg cgttttctgc tatcatgcgc ggcatgaaca tacttctctc
     3841 tattgcaatc acaacaggca ttctctccgg tatctgggga tgggtggctg tttccctggg
     3901 cttactaagc tgggcgggct tcttgggctg tacggcctac tttgcctgcc cgcaaggtgg
     3961 gctgaaaggg ctggcgatct ccgctgcaac cctgcttagt ggcgtggtgt gggcgatggt
     4021 cattatttac ggtagtgcac tggcaccaca tctggaaatt ctcggttatg tcataaccgg
     4081 tattgtggcg tttctgatgt gtattcaggc caaacagctg ctgctttcat ttgttccggg
     4141 gacgtttata ggcgcatgcg cgacgtttgc cgggcagggt gactggaaac tggtgttacc
     4201 ttcgctggcg cttgggctgg tatttggtta cgcaatgaaa aacagcggcc tgtggctggc
     4261 ggcgcgtagt gcaaagacgg ctcaccgtga gcaggaaatc aaaaataaag cgtgaggggc
     4321 actcacgctt tcgcttaaac agtaaaatgc cggattatta attccggcat ttttatctgt
     4381 caggattctg gtggaaccga catatggcgg tatttaacca gaatgtcatt ctgccgttct
     4441 gctttgttct gcaaatccca cagaccacgg tcgataccat cattaatcag gaaaatgacc
     4501 cctgtttcga tagccgacat cagacacagc ataacgggtt cgtttgaggt gtaacccact
     4561 tccccttcca gcaagcgctg gtagtcaata aagcggaaaa ccccggcctg aacttcatag
     4621 gaaagtatcg tcttactggt gttcaccgag gagaggatct caccggtact cacattgacg
     4681 acacgcaggt tcacggcaat ctgatcgagc tggtattgcg tgtctgcacc gatcccaaaa
     4741 tatcttgccc caacaccgcc cgatttaacg ttgctttcat aaccgataat cgaaccttca
     4801 accatgatat ttgccgccgt taaagattgc agcgggattc ggttattaat ggcaactgtg
     4861 ccgttttctt gtgccgcacg aataatcttg cgctcgttaa gcaggttttg taaactctga
     4921 cgctccagcg gtataaacca gcgagaatct ttcaatgccg tgaccagcat tgccgtggcg
     4981 ctttgcggaa cagcagtgga gaagttactt gccgggtagg gtttaaattg cccggtttcg
     5041 tcctgaatgt tgtataccga aacaaagatc ttacccgtcg gcgctggcag atgggtcaaa
     5101 tctttgtagc tctgagcttg aggcattaat gtcggtctgg cggcttcttt aggcggggcg
     5161 gttaagcatc cgctcagtaa catgacggca accaaaagaa ataagcgctg catgattatt
     5221 ttccttatga agctggggct taaaaatcgg ttgagttatt ttgtaaaccc gaaacctgga
     5281 tggtcgaggt ttgtccggtt ttacgatctg tcacgttcaa ctgcaattga ccatcgcggt
     5341 tagcaatatc aacaatataa tcgttggtca ccatgcggcc cggtttaccg gtattaatat
     5401 tcgacagtag cccacctaaa atttgtgact ggatggcctg agtaaagtta tctaacgctg
     5461 agggtgtttc aataccaaag tcatcgttat agctcggatc tttataagag ttttgggcct
     5521 gagcgctatt taataaaaaa gcgccattat ttgggttacc accaaagttt ggattacgga
     5581 actggaaagt catggttcca gcccaactta atggcgaaat aagcatgagt agaactactg
     5641 catgtttgac acgcattgca gcctccgaac aattttttat ttagaattca tcatgcgcca
     5701 aatcgcccgt acttaataac gcctgattta tctggcgacg atttagtgct tcttcagttt
     5761 gaatcagtgc aaagacgaca gttttctcga agtctctttt caacggaaat aaaaaagtct
     5821 ggaaaataac gtcctgattg accgttatag tgatccagct tccccatcgt gcactgggcc
     5881 tttcattaat cgttaagtta cccgtatagt cactttccca tttatcacta aaggctcggt
     5941 aaaaatcatg gccaatagat gaaacagtat ggtcagttag caatcccggg acttctacct
     6001 caacggcgtg aagattccct gcggcgaaca gaaattctgc cgccacaatc cagcgtaaat
     6061 aacgtttcat ggctttatcg cctgaggtta tcgtttgccc aggaaaccgc ttgtgtccgg
     6121 ttttttacgg ctatcttctt gaaaagatta taaagatgcg ttttaaccgt attttcgctg
     6181 atgaacaacg aacgagcgat ctcgttatta gacgcgccga tacgcagttt attcaggatc
     6241 tctttttccc gatgagtaag gagggctgat tccgtgctgt tataacgata gttacctgaa
     6301 tgcgtaatca agtagctggc aagcttttgc gtaaagtagc attcgccgcg caggacgcct
     6361 tgcaacccat tgacaacacg ttcttgatcc tccatggcat aaaaaacgcc gttgatatga
     6421 ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg tatttagcaa caatattttg
     6481 atattgttgt ttttcctgct caaagtatcc tgccaataat ggataagctt tttatccgct
     6541 tccatcatat ccagaagaat aatagagcct gaagatatat cgtc
//
LOCUS       DAZPJP010000217         6555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPJP010000217 DAZPJP010000000
VERSION     DAZPJP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.219"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHU9J_003786"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHU9J_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1907226.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHU9J_003787"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHU9J_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1907227.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHU9J_003788"
     CDS             2408..3157
                     /locus_tag="ACHU9J_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1907228.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHU9J_003789"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHU9J_003789"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1907229.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHU9J_003790"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHU9J_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1907230.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHU9J_003791"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHU9J_003791"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1907231.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHU9J_003792"
     CDS             6034..6507
                     /locus_tag="ACHU9J_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR1907232.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1609 a         1656 c         1658 g         1632 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg ttact
//
LOCUS       DAZPJP010000218         6514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPJP010000218 DAZPJP010000000
VERSION     DAZPJP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.220"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(300..1283)
                     /gene="zntB"
                     /locus_tag="ACHU9J_003793"
     CDS             complement(300..1283)
                     /gene="zntB"
                     /locus_tag="ACHU9J_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1907233.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(1297..1344)
                     /gene="ydaN"
                     /locus_tag="ACHU9J_003794"
     CDS             complement(1297..1344)
                     /gene="ydaN"
                     /locus_tag="ACHU9J_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1907234.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            1538..2770
                     /gene="dgcM"
                     /locus_tag="ACHU9J_003795"
     CDS             1538..2770
                     /gene="dgcM"
                     /locus_tag="ACHU9J_003795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1907235.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(2791..3354)
                     /gene="smrA"
                     /locus_tag="ACHU9J_003796"
     CDS             complement(2791..3354)
                     /gene="smrA"
                     /locus_tag="ACHU9J_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1907236.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(3684..4592)
                     /locus_tag="ACHU9J_003797"
     CDS             complement(3684..4592)
                     /locus_tag="ACHU9J_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1907237.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            4768..6078
                     /gene="abgA"
                     /locus_tag="ACHU9J_003798"
     CDS             4768..6078
                     /gene="abgA"
                     /locus_tag="ACHU9J_003798"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR1907238.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            6078..>6514
                     /gene="abgB"
                     /locus_tag="ACHU9J_003799"
     CDS             6078..>6514
                     /gene="abgB"
                     /locus_tag="ACHU9J_003799"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1907239.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPG"
BASE COUNT         1657 a         1657 c         1592 g         1608 t
ORIGIN      
        1 tttgcaaata aataagtgtc gtcatatttc cctccggtca ttgggtggac tcttaaaggg
       61 tagacgctga taaataacag gcaaaaaaaa gccgactcat caaagtcggc gtcgtacgaa
      121 tcaattgtgc tatgcagtaa ttcaaaaaag gaagtaagac aatatggagc gcaacgccca
      181 tcgcttgacg ttgcattcac ctgcaaggga gatattgccc tgaatgggta gagagtttat
      241 tgacttcgct caaactttgc ggcgtttttg tatacagaca gccggaaaaa ttgcttttgt
      301 tacaaccatt tactacggtg caaccataaa gcaacaccac caataagaac aactaacaga
      361 atacaaaaaa ttgaaaatcc gaattgccac ccgccgccag ggatcccacc aaggttgacg
      421 ccaaataacc cggtcagaaa ggtactgggt aaaaagacca ttgccatcaa cgacattgta
      481 taggtacgac gagctaaatt ttcctgcatc acctgagcga tttcatccgc catcacgcca
      541 gtccgtgcta tacaggcgtc gatttcgtca aggccgcgcc caaggcgatc ggcaatatcc
      601 tgcatccgac ggcgttggtc atcgctcatc cacggcaaac gttcactggc aagacgagca
      661 taaacatcac gttgcggtgc catatagcga cgcatcacaa ttaattgttt gcgcagcaga
      721 gccaggaatc cacgcggtgg aatttgctga tcaaggagat tatcttcaag gtcgataatt
      781 ttatcgtgca gctgctcgat aaattcgctg gaatgatcgg tcaacgcatc gcacacatcc
      841 accagccacc ctccgcaatc ggtcggaccc gtgccctctt ccagatcgct caccacatcg
      901 tccagcgcca gcactttgcg ttgtcgggtc gaaacaatta accgcccgtc catatataca
      961 cgcatggcga ccagttgatc ggggcgttca tcggtgctgc cgtttataca gcgcaatgta
     1021 atcagcgtgc cttcaccgag acggctgact cggggccgcg tgctctcgcc cgccagcgca
     1081 tcacgtacgt tattgggaag cagcggtgtt gtcgccagcc attgggcgct atcatggtgt
     1141 acataattaa ggtggagcca gcagggatgc gcttcatcaa tcacatctgt attttccagc
     1201 ggtttaacgc cgcctctacc atccagcatc caggcaaata ctgcatccgg gacattaacg
     1261 tccgatccct taatcgcttc cacagtgcct ccatcatcaa cgcattattt tgtagtctag
     1321 ccttctggcc ctgttacgca acatctcatc accccattac cctgaaatga ttaataaaat
     1381 tctgtctaaa ttgaatacaa aaagcaaaat gcttttccgt atacaaaccg tgtgaagtgt
     1441 taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt tttgcgatcg
     1501 gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca acttcaatac
     1561 cctggactta ctcaccagtc ctgtctggat cgtttcgccc tttgaggaac agttaattta
     1621 tgccaatagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc tacgaaccgg
     1681 tccctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc tgcaaaacca
     1741 acacgatatt atcgaaatcc tcactgttca gcgtaaagaa gaggaaacag cattaaactg
     1801 tcggcttgtt ttgcgaaagc tgacagaagc agaaccggtg attattttcg aaggtatcga
     1861 agcgtcggca acgctgggtt taaaagccag tcgctcggca aattatcagc gcaaaaaaca
     1921 aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga ttgacccctc
     1981 acgagatggc caaatcgtcg atgctaacct cgccgcgctc aatttctatg gttataacca
     2041 tgaaacgatg tgccagaaac atacctggga aataaatatg ctcgggcgtc gcgtcatgcc
     2101 tatcatgcat gaaatttcgc atttacccgg tggtcataaa cctctgaatt ttattcataa
     2161 gctggcggat ggttcgactc gtcatgtgca gacctacgcc gggccgattg aaatttatgg
     2221 cgacaagctc atgttatgta ttgtacatga tattactgag caaaaacggc tggaggagca
     2281 gctggaacat gctgcccacc atgacgcgat gaccggatta ctgaatcggc gacagtttta
     2341 tcacattacc gagccaggcc aaatgcagca tctcgccatc gcccaggatt acagcttgtt
     2401 gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt ctaaaggtga
     2461 tgaggtgtta tgcgccctcg cccgcaccct cgaaagttgc gctcgcaaag gcgatttggt
     2521 gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac tggataccgc
     2581 gctttcgctg gctgaaacta tccgcgtaag cgtggcaaaa gtgagtattt cgggcttacc
     2641 acgctttacc gtcagcattg gtgtggcgca tcacgaagga aatgaaagca tcgatgaact
     2701 gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct
     2761 ggcggcataa gccgcggatg cgtctcgaga tcaacgactg cgcttagcgt ggcgctccca
     2821 gttttcttgc ttcgcctgcg ccgttttacg tagtgcgacg taacacgccc cgctgccgcc
     2881 atgatgcggt agcgcagtgc aatatgcctg aacatcatca aattcggtca gccagcgcgc
     2941 cacatagctg cggacaatat tggcatgcga tttatcatcc cgtcctttgc catgaataat
     3001 cagcacgtta cgcaaaccat cagccatggc ttgttgaatg aaactgaaca tcattttgcg
     3061 gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat atttaccact
     3121 gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca gcggctgact
     3181 tagtgggatg atgtcgagaa atccggtggt gaggaaatta tcaagctgca gcgtgtcgat
     3241 acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatcggtag cacgtttcag
     3301 cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt tcatgttaca
     3361 tcctcccgca attaagagcg cgatatgata accagaccgg gtcggtccaa caacgtatta
     3421 cccaaaattc cagtaataag ttccaaatat tgccgatatt ttaagcaaaa tgcttatgca
     3481 tgattattca ttcacgatat taataatgta acttatattt tcgtgaaatc tgtcactgaa
     3541 gaaaattggc aactaaaggt taaaaccgtt ataacacagt caccggcgca gaggagacaa
     3601 tgccggattt aagacgcgga tgcactgctg tgtgtactgt agagtctggc ggatgtcgac
     3661 agactctatt tttttatgca gttttaactt tgcagatagc cgcattctcg ccggaattgc
     3721 gtgattaatg acgcggtcag tggtgtctgg cgactatcac gccgctgaat caaataatag
     3781 gccgctttcg gtaaaatttc gctaaccggc aacatcacca gcccctgtcc gtgcaaggga
     3841 tcgcagccca tttcttcagg cagtatgctg agaaaatcgc tttttgccac cagactgata
     3901 caggctgaga acgtctcgca gactacaccg acctgtggcg tttgcgcctg atcgtcaagc
     3961 aattcactca actgtttgta gtaactgccg tgtggcgtcg gcattgtcca gctgtaatcc
     4021 agtaactgtt tgatcgaacg ggcaccaatg gcggggtgtc ccgggcggca aaagatcgcg
     4081 aattgctttt ccagtaattt ctcaaaagta aattcgtggt cgtacggtcc ctgataatag
     4141 gtattgatgg tgaaatccaa ttctccctga cgcaattcat taatcatcga caccagttgc
     4201 ccttccataa tgcgtacttt tacctgcgga tgctgctgat gaaaacgaga tatgacagct
     4261 ggcatcagac tgcgggaaat actggccccc atgccgatat taatctgccc tgccagttgc
     4321 ccttgtcgtt ggcgaatatc ctcttgggct gcgcgcagct cttcaagaat aagactggcg
     4381 tgctgataaa aactttcacc ggcatcagtt aacgtcacgc ctttactacg gcgaaaaaag
     4441 agttgcgccg ctaatccttc ttccagctcc tgaatagatt tactcagtgc cggttgcgac
     4501 atattcaaca ttcggctcgc tccgcgaatg ctgccctgac gagccacttc aacaaaagcc
     4561 cgaatttgat gaatttttac ctgaaaagcc atgacgccac cgataaccgt tatttatcag
     4621 accaaagaaa ctggcatcta ctttaatgca gatgattgtg tcagggtaat ttatgaacgg
     4681 ttaaaactgt gaaaaatcag ttagtgataa gtaaaaacta tcgctacgtg aaccgggtca
     4741 cactttttac tgatgacggg aaaggttatg gagtctttga atcaatttgt taattcgctt
     4801 gccccaaaat tatcgcactg gcgacgtgat tttcatcact atgcagagtc tggctgggtg
     4861 gaattccgca ctgccaccct tgttgcggaa gaattgcagc agctcggcta ttcactggcg
     4921 ctgggccgcg aagtagttaa tgaaagtagc cggatgggac tacctgatga actcactcta
     4981 caacgcgaat tcgagcgcgc tcgtcaacag ggtgcgctag cacaatggat tgcggttttt
     5041 gaaggtggtt tcactggtat cgtcgccacc ctggataccg gtcgccccgg tccggtgatg
     5101 gctttccgtg tcgatatgga cgcgctggat ctcagtgaag agcaggatgt cagccatcgc
     5161 ccctaccgcg acggttttgc gtcatgtaac gccggaatga tgcatgcctg tggtcatgat
     5221 ggacataccg ccattgggct tgggctggcg cataccctta aacaattcga gtccggacta
     5281 catggcgtca tcaaactgat ttttcagcct gcagaggaag gtacgcgtgg cgcgcgggcg
     5341 atggtcgatg caggtgtcgt agatgatgtt gattatttta ctgccgtgca cattggcact
     5401 ggcgtacctg cgggcaccgt ggtgtgcggc agtgataatt ttatggcaac caccaaattt
     5461 gacgcgcact tcaccggtac cgccgctcac gcaggcgcaa aaccagaaga cggtcacaat
     5521 gccttgttgg cggcagcaca agccactctt gcactgcatg caatcgcccc gcacagcgaa
     5581 ggagcttcca gagtaaacgt gggcgttatg caggcaggaa gcggtcgtaa cgttgttcct
     5641 gcctcggcgt tgctgaaagt ggaaacacgc ggggccagcg acgtcattaa tcaatatgtt
     5701 tttgaccgtg cacaacaagc gattcagggc gcagcaacca tgtatggtgt cggcgttgaa
     5761 actcgtctga tgggtgcagc taccgccagt tctccttcgc cgcaatgggt cgcatggttg
     5821 caaagtcagg cggctcaggt cgcgggggtc aatcaggcca ttgaacgtgt tgaagcgcct
     5881 gcgggttccg aagatgccac attaatgatg gcccgcgtgc agcaacatca agggcaagcc
     5941 tcctacgtgg tgtttggcac acagctggcg gcaggtcatc acaacgaaaa attcgatttt
     6001 gacgagcagg ttctcgctat tgccgtcgaa acgctggcgc gcaccgcgct caattttccc
     6061 tggacgcgag gtatctgatg caggaaatct atcgttttat cgacgatgcg attgaagccg
     6121 atcgccaacg ttataccgat attgccgatc aaatctggga tcatccagaa acacgttttg
     6181 aagagttctg gtcagcggag catctggctt cggctctgga atctgcaggc ttcaccgtta
     6241 cccgcaacgt aggcaatatc ccaaatgcct ttattgcttc gtttggtcaa ggcaaaccgg
     6301 ttatcgccct gctgggagaa tatgacgccc tggcaggttt aagtcagcaa gctggttgcg
     6361 cgcaacctac atccgcgacg cccggtgaaa atggtcacgg ttgcggacac aatttgcggg
     6421 gaaccgccgc ctttgccgct gcaatagccg tcaagaaatg gctggaacaa tatgggcaag
     6481 gcggcacagt gcgcttttat ggttgtcctg gcga
//
LOCUS       DAZPJP010000219         6510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPJP010000219 DAZPJP010000000
VERSION     DAZPJP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.221"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(123..497)
                     /locus_tag="ACHU9J_003800"
     CDS             complement(123..497)
                     /locus_tag="ACHU9J_003800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907240.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(820..2754)
                     /locus_tag="ACHU9J_003801"
     CDS             complement(820..2754)
                     /locus_tag="ACHU9J_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1907241.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            2834..4768
                     /gene="yeaG"
                     /locus_tag="ACHU9J_003802"
     CDS             2834..4768
                     /gene="yeaG"
                     /locus_tag="ACHU9J_003802"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1907242.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4881..6164
                     /locus_tag="ACHU9J_003803"
     CDS             4881..6164
                     /locus_tag="ACHU9J_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR1907243.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6311..>6510
                     /locus_tag="ACHU9J_003804"
     CDS             6311..>6510
                     /locus_tag="ACHU9J_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1907244.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLT"
BASE COUNT         1819 a         1521 c         1346 g         1824 t
ORIGIN      
        1 gtacaaagcc tactatctcg taaagtaatt tagtaaacca ttccattttg aagtaccccc
       61 attcacattc aactttatag tatttagtga gggagtactt aaaacgtaac aatttggatg
      121 tgttagctaa taagttttag tataggtttc gaataaatga caagtccagt aactgaacat
      181 tttgcttctt ttatctcttc aattagttcc attgatattg gatgtttgaa agcttgagta
      241 cactcctgac agcctgcctc cgttagcgga aactcatctg ttataacttg aatttcagaa
      301 gaatacggca aaaaaatgta ctgaacttta attttgttgg aaaattcgta cttgcataag
      361 ctctcatctt gagaatcatt ccgctttgaa aaaagcctta attgattctc ttcattcaat
      421 gagatcccaa tatcataatt ctcagggaaa aattgacgga atttttcttt ttttgcttta
      481 ttttctttct tacgcatttt ggcaatcctc tctttagaat tcatctcatg gtttcatcgc
      541 tcctattcgt tagtgaatga cgctatggta ctggtttaac gttgaaaagt caaaaaagtt
      601 aattttaccc ttgactattc taacacacgc tgatataaat gatttgctgt attttgcacg
      661 gaaatcttaa acccatcggg tgcgtagtta tatccaaaat tcataatccc tcttttattg
      721 ctctatctac aatgccgtta gatttcgaca tcgtagatat attgttcaaa tattaactcg
      781 cataattcat tctacaagac cataaattct cattagcttt taatccatcc aatcaatcat
      841 atcttcatta acaatgatct caaatttttc acgcttgaga agattaagat acaatccaac
      901 atcatcaagt attttggtac ggctaatggc aagcgaccac atatcagtac cagatgtaac
      961 ggaggcaatg aaaacggttt tgccctcttc attgacagtg tagacacgct cgccgcttgt
     1021 ttcagagatt acgatctcat gtagtacttc acccccgccc cgcttgttat caatcacaag
     1081 cgtatgggat aggacatcat tgaagtattc caactgtaca aatatacgct tttcaaaaac
     1141 ccagtactta acagatttca gtactaatct tacttctttc cgtaactttg cacgtaactc
     1201 gacattcaat ggatcaagta ctttattaat atcgaaatca aatacaggga tttctttgat
     1261 gttctttgaa tttaatatct cagattctat ttcttcaatt ctatcctgta cctcagttaa
     1321 acccgtcatt aatggtaacg aggctttctt acctgctgcc ttacgttcat taattttaac
     1381 ctgataggtt tctgcctctt tctgtaaagt acttaactct tctcgcaatg atgcaagcaa
     1441 tgaatctgaa tccgacttac cttccatcat caatttattg aagtctacat ttttgacaag
     1501 ctgtagaatg ttcttttcaa ttctcatgta ctggatagat ttagcatcgc agatcttctt
     1561 ttcttccctg ttacgacaaa taacatactt agttcgtaca tgattatagt gaattgcaat
     1621 attgccccca cagtgaccgc atttcagcat cttcgggaag aggttagtaa tgtaatcatc
     1681 ctgagcaatg gtaatgtcct tacggatacg attagctata ccattccgcg ataacttctc
     1741 attagcccgt tcaaaggtga ggttatcgat aatcttcatg gtaactactt tcttatcacg
     1801 tagtaccctc agtacattga cggtactcca ccctttcggc aagcgtttta caatcgcccc
     1861 tgaaccaagg cctgtctcat aaagtttaaa aatctctcgg acaacttctg gttcaagtaa
     1921 tagatcctct gctggcttct tcttctccaa cataacgcga tccatcattc tattcgctgt
     1981 caggaacaac tcttcgtcga tgattttcgg gtaaacgttt ggtactacct caccgttgta
     2041 acgttcatgc tcgcccaaca aacggcgatc acgtatcagc ctggacacag tgaccatctg
     2101 ccactttcta ccgatacgtt taacaatctc gccagtactc aacccttccc tataccacat
     2161 gaaacattta acaatcagtt ctgcctgttc cggtataact tggtacttgt tatctacatt
     2221 tctcaaccaa ttaggcatac ggttggagat tacttccccg ttctgtactg cacgtgtgat
     2281 tttgttatcc catgccaatt ttgcacgtac cgatttcatc aatgactcat tgtgtgaacg
     2341 gtgctgtatc agttccatgt gaatttttga cataggatca tcaacccgta acacaaggtt
     2401 tgtagagata tcgtgaatct caatcccact attcacgatg tactggatag tactttcggc
     2461 ccagcttgat cgacgcgata gacgatccag cgataaaacc acaagtgcgt cacctacgcc
     2521 gtacttgcgg ttgcccacat cttgcaaaaa tatcccaagc tgagactctg gagagatatt
     2581 ggcatttttg aatgcggaaa gccccgcatc agtaatgaaa atcctttcgt cagtgaactt
     2641 atcagcattt ttatctaagt atgcagtcaa tgatgatcgt tggtcgtcca aacctttacc
     2701 catatcaact tgctgaatgc ttgatactcg ctcataacaa ataggacgga gcatattttt
     2761 tatcccgttg aagaattacg ctaaaagtgc tgattcacaa atgttcccag agtatacaaa
     2821 aggacggcat accatgaata tattcgatca ctatcgccag cgatatgaag ctgccaagga
     2881 cgaagagttc acactgcagg agtttcttac cacttgtcgg caagatcgca gtgcttatgc
     2941 caacgcggct gagcggctat tgatggctat cggtgagcct gtcatggtcg atacagccca
     3001 ggaacccaga ctttctcgac tcttttctaa ccgggtcatt gcacgttatc cggcatttga
     3061 agagttttac ggcatggaag acgcgattga acagattgtc tcttatctga aacacgcggc
     3121 tcaggggctg gaagagaaga aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa
     3181 atcatcgctt gctgagcgac tgaaatcatt aatgcagctc gtaccgattt atgtattgag
     3241 cgccaacggt gagcgtagcc cggtcaacga tcatccgttc tgtcttttca atccgcagga
     3301 agatgcgcag attctggaaa aagagtatgg cattcctcgc cgctatctcg gcaccatcat
     3361 gtcgccgtgg gcggcaaaac gcctgcatga atttggtggc gatatcacta agttccgggt
     3421 agtgaaggtc tggccgtcaa ttctgcaaca aattgctatc gccaaaacag aacccggtga
     3481 tgagaacaac caggacatct ccgcgctggt agggaaagtc gatattcgta aactcgaaca
     3541 ctacgcgcag aatgacccgg acgcctacgg ctattccggt gcgctgtgcc gcgccaatca
     3601 ggggatcatg gaatttgtcg agatgtttaa agcaccgatt aaagtgctgc atccgctgtt
     3661 aaccgctact caggaaggta actacaacgg gacggaaggt atctccgccc tgccgttcaa
     3721 cgggattatt ctcgcccact cgaacgaatc cgaatgggtc actttccgta ataacaaaaa
     3781 caacgaagcc ttcctcgacc gtgtttacat cgtgaaggtg ccgtattgct tacgcatttc
     3841 cgaagagatc aaaatctacg agaaattgct taatcacagt gaattgactc acgccccatg
     3901 cgcccctggc acgctggaaa cactgtcacg tttttccatt ctttcgcgcc tgaaagagcc
     3961 agaaaactcc agcatttatt caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac
     4021 cgatcccaaa gccaagtcgt atcaggaata tcgtgactac gccggtgtcg atgaagggat
     4081 gaaaggtctg tcgacgcgtt ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca
     4141 tgtagaagtg gcggcaaacc cggtccatct gttctacgtc ctagaacagc agatcgagcg
     4201 cgagcagttc ccgcaagagc aggcagaacg ctatctggag ttcctgaaag gttatctgat
     4261 cccgaaatat gccgagttta tcggcaaaga gatccagacg gcctaccttg aatcctattc
     4321 cgaatatggg caaaacattt tcgaccgtta tgttacctac gcggatttct ggattcagga
     4381 tcaggagtat cgcgatccgg ataccgggca gctgtttgac cgcgagtctc ttaacgccga
     4441 gctggagaaa atcgagaaac cggcggggat cagtaatcca aaagatttcc gcaacgagat
     4501 tgttaacttc gtactgcgcg ccagagcgaa taacagcgga cgcaatccga actggaccag
     4561 ctatgaaaaa ctgcgcacgg ttatcgagaa gaaaatgttc tccaataccg aggagctgtt
     4621 gccggttatc tcgtttaacg ccaaaacgtc aaccgacgag cagaagaaac acgacgactt
     4681 tgtcgaccgt atgatggaaa aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg
     4741 gtatttgcgc gtacgtaaat cgtcttaaca accctggccc ggtacacatg ctaccgggcc
     4801 tataacgaca gcgaaccgtg ggccagagaa atggcaacac aggcgtagca tacagttggc
     4861 aaatgtagta cggggggcat atgaccgggt ttattgaccg gcgtctgaac ggcaaaaaca
     4921 aaagcatggt gaatcgccag cgttttttac gccgttataa agcgcaaatt aaacagtcga
     4981 tctccgaggc cattaataag cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca
     5041 ttcccacgga agatattagc gaaccgatgt ttcatcaggg gcgtggcggc ctgcgccacc
     5101 gcgtacatcc gggcaatgac catttcgtcc agaacgaccg aattgaacgt ccccagggtg
     5161 gcggcggagg ttccggcagt ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg
     5221 aatttgtctt tcagatttcg aaagatgagt atcttgatct gctctttgaa gatttggcct
     5281 taccgaatct gaaacaaaac caacaacgcc agctgaccga atataaaacg catcgggcgg
     5341 gttataccgc taacggcgtt ccagccaata tcagcgttgt gcgttccttg cagaactcac
     5401 tggcgcgacg cacagccatg acggcgggca agcgacggga acttcatgcg ctggaagaga
     5461 atttggccat catcagcaac agtgaacctg cgcaactgct ggaagaggaa cgtctgcgca
     5521 aagaaattgc agaattacgt gccaaaattg aacgcgtccc ttttattgac accttcgatt
     5581 tacgttacaa gaactacgag aagcagcccg atccctccag ccaggcagtg atgttttgcc
     5641 tgatggacgt ttccggttca atggatcaat ccactaaaga tatggctaag cgtttttata
     5701 ttctgctgta tctgttcctc agcagaacgt ataagaacgt ggaagtcgta tacatccgcc
     5761 atcataccca ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag gaaacaggcg
     5821 gcaccattgt ttccagcgcc ctgaaactga tggatgaggt agtgaaagag cgttataacc
     5881 cggcacagtg gaatatttac gctgcacaag catcggacgg cgataactgg gccgatgact
     5941 ctccgctttg ccatgaaatc ctggcgaaaa aattattacc tgttgttcgt tattacagct
     6001 atatcgaaat tacccgtcgt gcacatcaga cattgtggcg agaatatgag catctgcaat
     6061 ctactttcga caactttgcg atgcagcaca tccgcgacca ggatgatatt tatccggtgt
     6121 tccgtgaact gtttcataaa caaaatgcaa cagctaaaga ctaaaactat cagccaggtc
     6181 attatcgcct ggctgatttt tagcttactg taaattatct catttattac atacattagc
     6241 ttacaatcgc tttaaatatg acagcataac ctttacataa tttagttcca gaaaacaatc
     6301 attcggaaaa atgattcagt caacacgtat ttccatgggg ttattcttta aatatttttt
     6361 atcgttaacg aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa taaaatccag
     6421 cactcacatt gctctccttt ttatggtttc tatgggtaca caaaaattaa aagctcaaag
     6481 cttttttatt ttcagtttat tgctgacgtt
//
LOCUS       DAZPJP010000220         6421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPJP010000220 DAZPJP010000000
VERSION     DAZPJP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.223"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHU9J_003805"
     CDS             <1..331
                     /locus_tag="ACHU9J_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1907245.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHU9J_003806"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHU9J_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1907246.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHU9J_003807"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHU9J_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1907247.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGSLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHU9J_003808"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHU9J_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1907248.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHU9J_003809"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHU9J_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1907249.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHU9J_003810"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHU9J_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1907250.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHU9J_003811"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHU9J_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1907251.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1747 a         1514 c         1549 g         1611 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaact
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac tgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     6361 ataaatatca tttgttacgc cttaagtaaa accttaatca aataattctt ctgaaatgat
     6421 a
//
LOCUS       DAZPJP010000221         6391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPJP010000221 DAZPJP010000000
VERSION     DAZPJP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.224"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..356
                     /gene="ydjO"
                     /locus_tag="ACHU9J_003812"
                     /pseudo
     CDS             1..356
                     /gene="ydjO"
                     /locus_tag="ACHU9J_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="ACHU9J_003813"
     CDS             complement(360..1751)
                     /gene="tcyP"
                     /locus_tag="ACHU9J_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1907252.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1884..2474)
                     /locus_tag="ACHU9J_003814"
     CDS             complement(1884..2474)
                     /locus_tag="ACHU9J_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1907253.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="ACHU9J_003815"
     CDS             complement(2637..3305)
                     /gene="hxpB"
                     /locus_tag="ACHU9J_003815"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1907254.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3452..3988
                     /locus_tag="ACHU9J_003816"
     CDS             3452..3988
                     /locus_tag="ACHU9J_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1907255.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4029..4889)
                     /locus_tag="ACHU9J_003817"
     CDS             complement(4029..4889)
                     /locus_tag="ACHU9J_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1907256.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="ACHU9J_003818"
     CDS             complement(4995..5285)
                     /gene="ghoS"
                     /locus_tag="ACHU9J_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1907257.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="ACHU9J_003819"
     CDS             complement(5386..6315)
                     /gene="pfkB"
                     /locus_tag="ACHU9J_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1907258.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1658 a         1596 c         1563 g         1574 t
ORIGIN      
        1 tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag tttccttcga
       61 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
      121 gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa cgtctcgggt
      181 atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt attgataagt
      241 tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg
      301 aacatttaaa aagtaaagca acggccccta tacaaaacgg accgttgcca gcataagaat
      361 taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc
      421 agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg
      481 cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc
      541 atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct
      601 gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta
      661 atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg
      721 ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc
      781 agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta
      841 aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta
      901 atgcccagca gaatgccatg cactgcaaac ataatcagca gaccgaggta ggacgcgaca
      961 acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac tttggtcatt
     1021 agagccagaa cgccgtaagg ggtcaactgc atgacaaggc gaaccagttt catcacccag
     1081 ctttgtaggg tatcgatagc ggctaagacg cgttcacctt tcggcgcatc atccttcagc
     1141 agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc
     1201 ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc
     1261 ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca
     1321 gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt ggtgaccagc
     1381 acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt cagaaaactg
     1441 attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat agaggcgaag
     1501 actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac gatgttaaac
     1561 cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg cagggcaagg
     1621 ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact ccactgttta
     1681 tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt cgcaattaat
     1741 ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag cagtcatagt
     1801 gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga attgcttatt
     1861 ccagaaaggt gatttttgcc tttttagtat tcaacctgat gcttaataag ccgatgaaac
     1921 tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt ctcgggtaat
     1981 gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg ttccagttgg
     2041 ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg ccagagcagg
     2101 ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc cagcaccatt
     2161 ccttgtagcg catcagccgg aatgaaccag ccttccggaa ccttaaggta aaaggttgcc
     2221 agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa agcgcgggcg
     2281 atcggttttg atatccattt taatcgctgc ccaagaaacg actttgggtg atcgatgtcc
     2341 ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc ctgtgccagc
     2401 acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa gagaaggtga
     2461 ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata ggatttttat
     2521 acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat taatccgtca
     2581 gttgaatgca aattcagata aagaaccagg ggcagcgtga tcactgcccc tggtcattaa
     2641 ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt tgctaataca
     2701 aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat gcgggctgct
     2761 ttagaagcga tcatgccatt taccgagtct tccagcgcca cgcaggtcag tgggtcaacg
     2821 cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta aggcagtttt
     2881 tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa cactttttcc
     2941 agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc tttgcataac
     3001 gcaacagctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag tgaaatggca
     3061 cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg ttgccgggcg
     3121 taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag ctcgttacga
     3181 cgggagatat ccacccccag gcttgccatc acatccagtt cggctcgatc ccataaaggt
     3241 tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg acgcggggtt
     3301 gacattacaa cctctccttt agagcattat gtgtaaggta ttaagccgtg cttatgatta
     3361 agcgcctatc atatcgcctt taaagaatgg gcgaaaacag atatctacag gtaaactaag
     3421 tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg gacgcattgc
     3481 tgttttgaaa aggattttgg gttgggtgat ttttatacct gcattgatct ccacattgat
     3541 ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta atgcggtcat
     3601 gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt ttttgaatct
     3661 gttctggtat aactccccga cccctaattt taatggtggc gtgaacgtta tgttctgggt
     3721 gattttcatc ctgatttttg tcggactggc gttgcaggat tccggtgccc ggatgagtcg
     3781 ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa aagccaaagg
     3841 ggaagagggg ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc accatactat
     3901 tttcctgcag tttttctcac tatatatcct gccggttatt tgtattgctg caggttatgt
     3961 gttcttttct ctgcttgggt ttatttaatc aaaaggccgc acggagcggc cattatcctc
     4021 aacccatatc atgctgctaa taatctatcc aatgactgct gagcaataac caaatgctga
     4081 ccgccaaata atcttgcacg atttaacagc gtgtagagtt ggtaaaccgg ttgacgttca
     4141 aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg tggcggctgt
     4201 tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta gcaggcaggg
     4261 tcgaaaatgt acgggccatc cggacccagc gcacagttgc cggaccataa atcgccgtgc
     4321 aacagagaag gctgcagttg atgcgaggcc agacgctgct gaatatgctc gacgatggcg
     4381 tcgatattgc cgaaagcgat ccctttctct gctgccagtt ccaactgcca gccaatccgt
     4441 tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg agttgtggag
     4501 agcgagttat caaaatcgag gccaaattgt ggttggtcac tccattgatg taaacgcgca
     4561 atttgctgac caagaataaa tgcgctatgc gcatccagcg gacgaggtgg gagataatcc
     4621 atcaccagaa aactgtagtc acggtcagcg cctactgccc aaaccttagg cacggtgacg
     4681 gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc gggaagcagt
     4741 tccctttcat cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg ccatgcggca
     4801 tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc taactgctcg
     4861 ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct gccagatcat
     4921 aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca gactgattat
     4981 tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaagtcgt gatggtgatt
     5041 tcaggatctt tgccggttgc actttgggta agccccgaaa ccagttctct aatttcttct
     5101 tcactttggg tactgataag tccaaaagtg ttagtcccca gctcatggat atttccctca
     5161 tcatccgtca tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa ctcgttaatt
     5221 tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag atcgccactg
     5281 gccatagttc acccctttgt tatcacatga cttttgagca aagtcggaga aacgcgttgc
     5341 cgacaggttg gtggtgattc ccccaatgct gggggaatgt ttttgttagc gggaaaggta
     5401 agcgtaaatt ttttgcgtat cgtcatggga gcacagacgt gttccctgat tgagtgtggc
     5461 tgcactcccc gcagctacgc caaaacggac catctcttca agagaggcat tttctgccag
     5521 tttcagtgtc atcgcgccga ccatgctgtc accagcgcca acggtactct ggcttttcac
     5581 cggtggtggc accacctgaa tgcagttttc actatcaaca cccagcgctc cttgtggacc
     5641 aagggaaacg acaacccgtt tggccttgcc gctattaacg atttcctgcg cggctttgcg
     5701 gacatcgtcc ggctgggtga gttcgcgatt caccagcgca ctgagttctt tttggttagg
     5761 cttaaccaac tcgatgttac caattgccag tgctgcactt aacgcttcgc cagaactgtc
     5821 gacgatgcag cggatccctt gtttttgcga agcggaaatc agttgggtta atttttccag
     5881 cttcacacct ggcggcaggc ttccgcttat gaccaggatg gccccggatt caatttccag
     5941 aacttgctct tcaagctggc gaaactcatc ttcatttaat gccgcgcctg gcataacaaa
     6001 acgatactgc tcaccgcttg cttccacatg tacatgtaaa ttctgccggg tccagtcttt
     6061 ggcttctaca gtagcgacgg ggacattttc atccgccaac agtgaaacca ggtgttcgcc
     6121 ggtcgcgcca cccgccggga agatcgctgt ggcactgcct ccaagatggg caatggcgcg
     6181 ggcgacgttg atgccgccgc ccccgggttc gaacaccggt gcggtacagc gcagttttcc
     6241 ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa gtgtcaacgt
     6301 atagatacgt accatcattt cctcctatag gctgatttca gtctggcacc gaatcagcgg
     6361 aaagtgaaga aattaacaat atgatttatt g
//
LOCUS       DAZPJP010000222         6347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPJP010000222 DAZPJP010000000
VERSION     DAZPJP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.225"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            193..720
                     /locus_tag="ACHU9J_003820"
     CDS             193..720
                     /locus_tag="ACHU9J_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1907259.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     QQPEPPYGFDDDAPF"
     gene            777..1010
                     /locus_tag="ACHU9J_003821"
     CDS             777..1010
                     /locus_tag="ACHU9J_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR1907260.1"
                     /translation="MNKSDEMIQELPDGAFTRECAETVVTQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGIRK"
     gene            1072..3030
                     /locus_tag="ACHU9J_003822"
     CDS             1072..3030
                     /locus_tag="ACHU9J_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1907261.1"
                     /translation="MSVVKSEPDTTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILTADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEYLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEIRAGSGVVVSWCYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSSPARIYLECGHYSLTSAAPSGKEGTAFMALMAEHFRL
                     AALLPDEWERDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKQPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            3085..3519
                     /gene="psiB"
                     /locus_tag="ACHU9J_003823"
     CDS             3085..3519
                     /gene="psiB"
                     /locus_tag="ACHU9J_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1907262.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3516..4235
                     /locus_tag="ACHU9J_003824"
     CDS             3516..4235
                     /locus_tag="ACHU9J_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR1907263.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            4232..4828
                     /locus_tag="ACHU9J_003825"
     CDS             4232..4828
                     /locus_tag="ACHU9J_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907264.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
     gene            5291..5791
                     /locus_tag="ACHU9J_003826"
     CDS             5291..5791
                     /locus_tag="ACHU9J_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFR1907265.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(6103..6171)
                     /locus_tag="ACHU9J_003827"
                     /pseudo
     CDS             complement(6103..6171)
                     /locus_tag="ACHU9J_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
BASE COUNT         1319 a         1724 c         1962 g         1342 t
ORIGIN      
        1 aaaaaatttt gcgtccgccc ccggacagct gtcccgtgag gcggtaaacg ggcccccatc
       61 ggaaaggtcc actgcgggga gaaaccccga agaagtggac ggcaggagga gctttcgctc
      121 ctctgaaggg gcacatgaac gtcccgtcgc cggtatccgg tttatctcac atctttcagt
      181 caggaggttc ttatgagtgc acgtggtatc aacaaggtca tcctcgtcgg gcgtctgggc
      241 aatgatccgg aagtccgtta catccccaac gggggcgcag tggcaaacct gcaggtggcc
      301 acatcagaaa gctggcgtga caaacagacg ggggagatgc gggagcagac ggaatggcac
      361 cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg caagggtgcg
      421 caggtctaca tcgaaggtca gcttcgcacc cgtagctggg aagataacgg tatcacccgt
      481 tacgtcactg aaattcttgt taagaccacg ggcaccgtgc agatgctggg acgtgcacca
      541 cagcagaacg ctcaggcgca accgaagcct cagcagaatg ggcagccaca gagtgctgac
      601 gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc gcagccagag
      661 cctcagcctc aacaacagcc agaacctcct tatggcttcg acgatgatgc gccattctga
      721 tctggctgat ttttctgaca gccccaccat gtgtggggca ttaccggaga acgatgatga
      781 acaaatccga tgaaatgatt caggaactac ccgatggggc attcacccgc gagtgtgctg
      841 aaaccgttgt tacacagtac cggaatgtct ttatcgagga tgatcaggga acgcattttc
      901 gcctggttgt ccgtcaggat ggcacgttga tctggcgctc ctggaatttt gaggactgtg
      961 ccgggtactg gatgaaccgg tacatcaggg atttcggcat tcgtaagtaa gagaggtgcc
     1021 ggacggggat gacgtccggc agacattaac taaaccgtaa ggggaataat catgtcagta
     1081 gtaaagtctg aaccagacac cacccgcaaa gcaagccgta aatctgcaaa aacgcaggaa
     1141 accgtcctgt ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct ggcctcgctg
     1201 attaagtcac cgctgaatgt gcgcacggtg ccgtattctg cggagtccgt cagcgaactg
     1261 gcggactcca ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc cctgccaggt
     1321 gaccgttacg gtgtcgccgc aggcggtcgc cgactggcag cactcaacat gctggcagag
     1381 cgcaacatcc ttacggctga ctggcctgta cgcgtgaagg ttattccgca ggaactggcg
     1441 acggcggcat caatgactga aaacggtcat cgtcgggata tgcaccctgc cgaacagatt
     1501 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cgcaaatcgg tgatttgctg
     1561 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg cagaccttgc gcctgtcatc
     1621 ctcgatgcgc tggcagaaga ccgcatcacc acagaacact gtcaggcgct ggcgctggag
     1681 aacgacaccg cgcgtcaggt gcaggtgttt gaagctgcct gtcagtcggg atggggcggt
     1741 aaaccggatg tgcgggttat ccgcaatctg attacggaaa gtgaagtggc ggtggcgggg
     1801 aacagtaaat tccgcttcgt gggggctgat gctttctcac ctgacgaact gcgtactgat
     1861 ttgttcagcg atgacgggga cggttatgtg gaccgtgtgg cgctcgatgc tgctctgctg
     1921 gaaaaactcc aggctgtcgc agagtacctt cgggaagccg aaggctggga gtggtgcgcc
     1981 gggcgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     2041 gagccggaag cggtgctgac ggaggcggaa gaagaacgcc tgaacgaact gatgacgcgt
     2101 tacgacgcgc tggaaaatca gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg
     2161 atgcgctgca tggcgaaggt cagggcgtgg acgccggaga tacgtgccgg aagcggtgtg
     2221 gtggtgtcct ggtgttatgg caacgtatgt gtccagcgtg gtgtgcagtt gcgcagtgag
     2281 gatgacgtgg ctgacgacgc tgaccgcacg gaacaggtgc aggagaaagc atcagtggag
     2341 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggcg
     2401 gcactcatgc agcaaccgga caaatctctg gcactgctgg catggacgct ctgcctgaat
     2461 gtgtttggca gcggggcgta cagtagtccc gccagaattt acctggaatg tgggcattac
     2521 tcactgacca gcgctgcgcc gtcagggaag gaaggtaccg cattcatggc gctgatggca
     2581 gagcactttc gccttgctgc cctgctgccg gatgaatggg aacgggacat gacgacgttc
     2641 ctgtcactca gccaggaggt tctgttatcc ctgctcagtt tctgcactgc atgcagcatc
     2701 cacggcgttc agacccgtga gtgtggtcac acgtcacgca gtccgctgga ctcgctggaa
     2761 accgccatcg gctttcacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga
     2821 cacctgaaac agccgcagat tatcgcagcc ctgaatgagg ccggactgtc cggtgccgca
     2881 cgggacgcgg agaaaatgaa gaaaggcgat gcggctgaac atgcagagtt ccatatgaaa
     2941 gacaaccgct gggtgcctgg ctggatgtgt gcaccacgtc cacagacgga tgccactgaa
     3001 cacaccgcta acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
     3061 ggcggcagca agggagatac cgtgatgaaa actgaactga ccctgaatgc cctgcagtcc
     3121 atgaacgcac aggaatatga agatatccgt gctgcgggaa gcgatatgcg ccgtaatctc
     3181 actcacgagg tgatgcgtga agtggacgca ccggctaact ggatgatgaa cggagagtat
     3241 ggcagtgagt tcgggggctt tttccccgtc caggttcgtt tcacgccagc ccacgaacgt
     3301 ttccacctgg cattatgttc gccgggagac gtctctcagc tctggatgct ggttctggtg
     3361 aattgtggtg gacagccttt cgccgtcgtt caggtgcaac atatcttcac gcctgtcgct
     3421 atcagtcaca cgctggcgct tgccgcgaca cttgatgcgc aggggtacag tgttaacgac
     3481 atcatccata tcctgatggc agaaggaggt caggcatgag cgcacgttca cgggcactga
     3541 tccccctcag cgcagagcaa caggccgcca tgcaggctgt ggctgtcacc gaacaacgtc
     3601 gtcgtcaggg acgcacactt tcagcatggc cttatgccag cgctttcttt cgctgcctga
     3661 atggcagtcg ccggatttcg ctgaccgatc tccgcttttt tgctcctgcg ctgacgaagg
     3721 aggaatttca tggcaaccgt ctcctgtggc tggctgccgt ggataaactg attgaaagtt
     3781 ttggtgaagt ctgtgttctt cccctgccat ccgatgcggg gcatcgtctg ttcccgtccg
     3841 ttccttttcg tgaaggtgag cggcgtcgtc agaaaaccac gctgacagag cagaaataca
     3901 gccgccagcg ggaacgtgag gcagaacgac gggaactgga ataccagaca tgttttgctc
     3961 aggcgcagat tgaccttgcg tttcatactc ccgccacggt cggaagctgg ttgtcccgct
     4021 ggtctggtgt tgttgaggag catgatctgg aaacgatttt ctgggggtgg tgcgggcgtt
     4081 ttccatcact gtcatcattt gaccggtttt tctggcagga ggaaccactc tggcggctga
     4141 tttttgaagc cggtgaggcc ggtcgtggtg caccggtaca ggtacgtgca cttgagcagt
     4201 ggatgatccc gaacaagctg gagaacgcaa tatgatgaaa tcagacgaac aatatcaggt
     4261 tcccgtgtgg atgcgacctc tgttgccgtt gctctgcaat accggtggta atgatccgga
     4321 ggaactgctg aatgatacag aaaccaccgc cagtgcgaat attgtccgtt atgtactgat
     4381 agttgcagtg cggtcgcaga ttgatctgct gcagctactg tacaggaaag gactgttgcg
     4441 cacagagata ccaggtggct tttcaccgga agaagcgcag gaactcctgg ataatctggt
     4501 gcgcagccat atcagcaagg cgctgtcggg tgagcgaatg gcagcccgtg acagaaatgc
     4561 cgatctgacc tggattcgcc agcaactggt cgatgccgcc tggtttgtcc gtgccacact
     4621 ggaagcgcat gggatgggcg tcggaaatga gagtccctct gctccgccgg agacaatgcc
     4681 ggacatacag acacgggaac tggttatgtt gatcaagcga ctggcatcat cgctgaaagc
     4741 ggtgaaaccc gacagtagcg tggtgcgtga agcgcaggac tggctgtgcg acagaaaact
     4801 tgtggatatc acagatattc tccggtgaac cagacatgta tattttacgc agggacacgc
     4861 cgatcatgat cgtttcatat caggggtggt ctggaggtca tgcggcgtgt cctctgcact
     4921 cgccggaata aggaagtcgc cggcggctcc gcttttaccc ggccatgcgg ggcatggcct
     4981 tgtgggtttt cagctctgtg gcctcagcgt cgtgtgcggg ctgtgccgtg cctccatctt
     5041 agccgggctg gcagggatgc aagggtacgc ttcgccgctg cggtcacccg gtccctcctt
     5101 cccgtctgcc gtgattttcc ggtcgttcac ccgctcagat ttcgcggtct caccctccaa
     5161 ctcccgccag ccgtgtgcgt caggctgcgg cttcccttgc gccctgtgca tccccgcctt
     5221 atcgcccggc ttttatggag gcacggcacc gcccggtgcc gcagaacatg tgactatgga
     5281 ggattcggga atgtctgttg ttgcacctgc tgtatacgtt ggaacctggc acaaatacaa
     5341 ctgtggaagc atcgccggac gctggtttga cctgaccacg tttgatgatg agcgcgactt
     5401 tttcgccgcc tgccgtgctc ttcaccagga tgaagccgat cctgaactga tgtttcagga
     5461 ttatgaggga ttcccgggga atatggcctc tgaatgccat atcaactggg cctgggttga
     5521 aggcttccgc caggcacggg atgaaggctg cgaagaggct tatcgtctct gggtggagga
     5581 taccggtgag acggattttg acaccttccg cgatgcctgg tggggcgagg ctgacagtga
     5641 ggaggctttt gcggttgagt tcgccagtga taccggcctg ctggctgacg tgccggagac
     5701 ggtggcgctc tattttgact atgaggcgta tgcgcgggat ttattcctgg actccttcac
     5761 ctttattgac ggtcatgtgt tccgtcggtg atttactccc ccgctccggc gggggtatgg
     5821 cctgccaggc cgccggaaaa ccctggcagg cttacgcctg cctgaaatgc aggagggccg
     5881 cgcctgcggc gctgcccttc ctgcccgtca tggtgtgagg catttttctg ttatgtcgtt
     5941 tctgttgttc tgatttccgg aggcggggac accttcggct cccggctgcc tggcggcatc
     6001 cggtcgtcag gcccgactcc gacaggcagc tgctggcgca cccgccagtc tccgccgaac
     6061 ccgctggcgc ggtttcgggc tgccgctacg gtccgatgcg tgaaaacccg ggcttgcagc
     6121 gaccgttacg gtcgcgccgc ccctgtcgcg ccgtgacctg cgctccctgc acacctgacg
     6181 gtgtgtgagg aaactgcgcc tgatggcgca gcagtcctcc ctcctgccgc cacccctgcg
     6241 gcctgtttcc cgctgtggac tttccggggg gcagacgtaa ccccctcccg ttccatctgt
     6301 ccagggtgag atgcaccggc cacaaaattt ttagccatga acccggc
//
LOCUS       DAZPJP010000223         6342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPJP010000223 DAZPJP010000000
VERSION     DAZPJP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.226"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="ACHU9J_003828"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="ACHU9J_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1907266.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="ACHU9J_003829"
     CDS             2218..2766
                     /locus_tag="ACHU9J_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1907267.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="ACHU9J_003830"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="ACHU9J_003830"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1907268.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHU9J_003831"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHU9J_003831"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1907269.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHU9J_003832"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHU9J_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1907270.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1594 a         1557 c         1609 g         1582 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa gg
//
LOCUS       DAZPJP010000224         6287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPJP010000224 DAZPJP010000000
VERSION     DAZPJP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.227"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..357
                     /gene="mcbR"
                     /locus_tag="ACHU9J_003833"
     CDS             <1..357
                     /gene="mcbR"
                     /locus_tag="ACHU9J_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1907271.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
     gene            complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHU9J_003834"
     CDS             complement(393..2495)
                     /gene="pqqU"
                     /locus_tag="ACHU9J_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1907272.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2737..3798
                     /locus_tag="ACHU9J_003835"
     CDS             2737..3798
                     /locus_tag="ACHU9J_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1907273.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHU9J_003836"
     CDS             complement(3913..5412)
                     /gene="ansP"
                     /locus_tag="ACHU9J_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1907274.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5679..>6287
                     /locus_tag="ACHU9J_003837"
     CDS             5679..>6287
                     /locus_tag="ACHU9J_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1907275.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNE"
BASE COUNT         1693 a         1593 c         1504 g         1497 t
ORIGIN      
        1 ctcaccccgc aagacctgac ggaactccag gaattgctcg agaaattaca gcaggcgcaa
       61 gaaaagggcg acatggaaca aatcattaat gtaaacaggc tatttcgctt agcgatttat
      121 catcgctcaa atatgcccat cctgtgtgag atgattgagc aactgtgggt caggatgggg
      181 cctggtttac attatctcta tgaagcgatt aatccagcgg aattacggga gcatatagaa
      241 aactatcatc tgttactcgc tgcgttaaaa gcaaaagaca aagagggatg cagacattgt
      301 cttgctgaaa ttatgcaaca aaatattgct attttatacc agcaatacaa tcgttaaagt
      361 aaatgcccgg caaataccgg gcatcaaggt aattactcaa atctccacgc aatattcatg
      421 ccgacaccat aatttcgtcc gggcgaaggt tcgtaatatc gcccgtttga ctcattgaca
      481 atgacagaac caacgtattc tttatcgaat aaattatcga cacgaccaaa taaatccaca
      541 gttaaattgt ggtaattgta tttatacccg gtgaataagc cgacgagagt ataagacggc
      601 gcttttgccg tattttcatc atctgccata atatcgccca tataacgcgc ttccgtgcct
      661 gcataccaac catcttccgg tacataacct atcgacgcaa agcccatatt acgggcgatc
      721 cctggcatcc gattaccgtt acaatcctgt tcattgcaaa cattgctgcg ataggtcgca
      781 tcaagccagg tccaggacgc gtttacgcga aaatctcctg cgaaacgttg atcccatgcc
      841 agttcagcgc cttgacgacg ggtctttccg gcatttttgt aagtcgtacg cccaccgcta
      901 ctgctatcga caacaatttc atcatcagtg tcggtctgaa acaatgcgag actaagcagc
      961 ccatcaccaa tacgcgtttt actgccgatc tcaattgtat cgttggtgga tggttttaaa
     1021 ccaaagttca taccgctttg cccatcagca cgataagaca gctcattaat cgtcggcgtt
     1081 tcaaaacctc gcccggctgc cagatagata ttccaggcat cggtcattgc atattttaac
     1141 gaaccggcag gtagccattt atgataactg gcatcaccgc tgtcatcgcc gttacccgga
     1201 gtaacgtaat ggtcgttgga atcaaaccac acggagctgt agcgcacgcc agcatccagc
     1261 gacagttttt cgctcagctg ccactgcgtc tgtaaatagg gatcgatgtt ccacatcaga
     1321 ttgcgttcgt cgcgacgcaa ctcacctttt tgcccgtact ccggcatgcc gctattcagg
     1381 cggaagttat tgtagccctt gcggttttca ctcatgtttt cgtagttcag gccggtagtg
     1441 aacgtgaccg gaacgcccag ttcaccacgg tgtgtccagc ggctgtctat cccctggtaa
     1501 tggcgttgca gggtaatcac gccgcccgca tgtgacgggt taagttgtgg tgccatgggt
     1561 attgactggt actgggtcgt ttctcgctct ccggcataca tcatcacact catatcatct
     1621 tgcgcgctca ggctacgctc atagcgcaac ccagcctgag tttgcttgat ggtttttcgc
     1681 gtgtcgtact gttctgcacg aggcgcttgt tgtggattag ccttccattc tgctttggtt
     1741 agcccacccg ggtcatctgc tttgatatcc acactattga aaatcagact taatttgctg
     1801 gcttcatcaa tgcgtacgcc cagtttggca ttggctaaat ttttctgtgc gccactatgg
     1861 tcacgatagc cgtgggtcgt aaaacgcgtg gttgagacgg tgtaatcgac atcgccaggc
     1921 tgtgtgccgt ctcccgttgc gcccgttgct ttcagcccat agcgccagct gccaaaactg
     1981 ccgtagtaac tactggcttc aatggttggt ggctgttgtc cggtctgggt ggtgacattc
     2041 attaccccac cagacgcgtt gccatacagg gcagagaagg ggccacgcag cacttccaca
     2101 ttttgcacac tgcttaaatc gatgttggat gtttgccctt gcccgtcggg catggtggcg
     2161 ggaataccgt ccacatacag gcgaataccg cgaataccgt aagtggagcg ggagccaaat
     2221 ccgcgaatcg acagctgtaa atcttgcgca tagttctgcc ggttttgtac ctgcaaacca
     2281 ggcacgtcgg tcagtgattc ggacaagtta atgcgcggtg ttgccaggcg catctcctcg
     2341 ccatccacca cgcttactgc tgctgggata tccagttctg aaaccacctg cggtgcggca
     2401 ctgacaatca tggtctgttc atcagcggca aaaacaacgg gggaaagggc aagtagtgcg
     2461 ggcaaaacgg tctgtcggac ggaaaaaatc ttcatgaaaa aagccaggtt aagaatggga
     2521 aaacgccgtt atggtaataa aattgtaaat tcatggaaaa cgaaacggca cgatacgtta
     2581 agtaattgag aaaattgtag tcgtaacggc aagaaatgct ccacatttga gaaaataatg
     2641 attaccattc ccatttataa caagagcgta acgatgatta cgcttagcga agcattgtga
     2701 agcagcaaaa atatcggttc atcaaaggga gtcgtcatgc atttacgtca tctgttttca
     2761 tcgcgcctgc gtggttcatt actgttaggt tcattgcttg ttgcttcatc attcagtacg
     2821 caggccgcag aagaaatgct gcgtaaagcg gtaggtaaag gtgcctacga aatggcttat
     2881 agccagcaag aaaacgcgct gtggctcgcc acttcgcaaa gccgcaaact ggataaaggc
     2941 ggcgtggttt atcgtcttga tccggtcact ctggaagtga cgcaggcgat ccataacgat
     3001 ctcaagccgt ttggtgtcac catcaataac acgactcaga cactgtggtt tggtaacact
     3061 gtaaacagcg cggtcacggc gatagatgcc aaaacgggcg aggtgaaagg ccgtctggtg
     3121 ctggatgatc gtaagcgtac ggaagaggtg cgcccgctgc aaccgcgtga gctggtagct
     3181 gacgatgcca cgaacaccgt ttacatcagt ggtattggta aagagagcgt gatttgggtc
     3241 gttgatggcg agaatatcaa actgaaaacc gccatccaga acaccggtaa aatgagtacc
     3301 ggtctggcgc ttgatagcaa aggcaaacgt ctttacacca ctaacgctga cggcgaattg
     3361 attaccatcg acaccgccga caataaaatc ctcagccgta aaaagctgct ggatgacggc
     3421 aaagagcact tctttatcaa cattagcctt gataccgcca ggcagcgtgc atttatcacc
     3481 gattctaaag ccgcagaagt gttagtggtc gatacccgta atggcaatat tctggcgaag
     3541 gttgcagcac cggaatcact ggctgtgctg tttaacccag cgcgtaatga agcctacgta
     3601 acgcatcgtc aggcaggtaa agtcagtgtg attgacgcga aaagctataa agtagtgaaa
     3661 acgttcgata cgccgactca tccaaacagc ctggcgctgt ctgccgatgg caaaacgctg
     3721 tatgtcagtg tgaaacaaaa atccactaaa cagcaggaag ctacccagcc agacgatgtg
     3781 attcgtattg cgctgtaata gatatggcga gggtgaaaac ggagagactt gcctgacttt
     3841 tatcacgccg catgcggcac accttcgtca gtaatctaaa tttgcccggc acgtagccgg
     3901 gcaattttgc aattagctcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3961 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     4021 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     4081 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     4141 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     4201 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     4261 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4321 gacatacaca accagtgtcg ccagaatccc ggcatacggc acatgctgac gactcatttt
     4381 cgccataaaa ctcggtgcgg aaccgcccat cgccatcgag cgcagaatac gcccggtgca
     4441 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4501 gccgatatat ggcataccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4561 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4621 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4681 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4741 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4801 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4861 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4921 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4981 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     5041 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     5101 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     5161 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     5221 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     5281 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5341 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5401 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5461 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5521 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5581 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5641 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5701 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5761 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5821 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5881 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5941 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     6001 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     6061 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     6121 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     6181 ggggggctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     6241 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaa
//
LOCUS       DAZPJP010000225         6205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPJP010000225 DAZPJP010000000
VERSION     DAZPJP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.228"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            57..1655
                     /gene="rtcR"
                     /locus_tag="ACHU9J_003838"
                     /pseudo
     CDS             57..1655
                     /gene="rtcR"
                     /locus_tag="ACHU9J_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1637..2395)
                     /locus_tag="ACHU9J_003839"
     CDS             complement(1637..2395)
                     /locus_tag="ACHU9J_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1907276.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2412..3242)
                     /gene="glpG"
                     /locus_tag="ACHU9J_003840"
     CDS             complement(2412..3242)
                     /gene="glpG"
                     /locus_tag="ACHU9J_003840"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1907277.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3287..3613)
                     /gene="glpE"
                     /locus_tag="ACHU9J_003841"
     CDS             complement(3287..3613)
                     /gene="glpE"
                     /locus_tag="ACHU9J_003841"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1907278.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3803..5308
                     /gene="glpD"
                     /locus_tag="ACHU9J_003842"
     CDS             3803..5308
                     /gene="glpD"
                     /locus_tag="ACHU9J_003842"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1907279.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5360..5965)
                     /locus_tag="ACHU9J_003843"
     CDS             complement(5360..5965)
                     /locus_tag="ACHU9J_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907280.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5968..>6205)
                     /locus_tag="ACHU9J_003844"
     CDS             complement(5968..>6205)
                     /locus_tag="ACHU9J_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907281.1"
                     /translation="CEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENPLK
                     QPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1542 a         1637 c         1603 g         1423 t
ORIGIN      
        1 ttccacaaaa ccaattcacc gataatgaaa tatcgtcttt tataaggata tctaagatgc
       61 gtaaaacagt ggcttttggc tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc
      121 agcgctggtc gaaatggcgt ccgacgcttt gtttatgcca gcaagaatcg ttagtcatcg
      181 atcggctgga attgttgcac gacactcgct cgcgctcgct attcgaaacg cttaaacgcg
      241 atatcgccag cgtttcgcca gaaacagaag tcgtgggcgt tgagattgaa ctgcataacc
      301 cgtgggattt cgaagaggtt tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc
      361 agccagaaaa agaagactat ttaattcaca tcaccaccgg cacccacgtc gcgcagattt
      421 gctggtttct gctggcggaa gcgcgttacc tgcccgcccg gctgatacaa tcttcacctc
      481 cgcgcaaaaa agaacagccg cgcggcgcag gtgaagtgac gattatcgat ctcgatttaa
      541 gccgttataa cgccatcgcc agccgctttg ccgaggaacg ccagcaaacg cttgattttc
      601 ttaagtccgg cattgccacg cgtaaccccc acttcaaccg catgattgag cagatcgaaa
      661 aagtggcgat caaatcccgc gcgccgattc tgcttaacgg tccaaccggc gcgggcaagt
      721 catttctggc gcgacgcatc ttcgagttaa aacaggcgcg gcatcagttt agcggcgcgt
      781 ttgtggaagt gaactgcgcc accctgcgcg gcgataccgc catgtcgacg ctgtttggtc
      841 atgtaaaagg cgcgtttacc ggggcgcggt aatctcgtga aggtttatta cgcagcgcca
      901 acggcggaat gttgtttctt gatgagattg gcgaactggg cgcagacgaa caggcaatgc
      961 tgctaaaagc cattgaagag aaaacctttt acccgtttgg cagcgatcgc caggtgagca
     1021 gtgattttca gcttatcgcc ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca
     1081 aatttcgcga agatctgtac gcgcggatca atctctggac cttcaccctg ccgggtctac
     1141 gccagcgcca ggaagatatt gaaccgaacc ttgattatga agtggagcgc cacgccacac
     1201 tcactggcga cagcgtgcgt tttaacaccg aagcgcggcg cgcctggttg gcttttgcga
     1261 cctctcccca ggcaacatgg cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga
     1321 tggccacctt tgccactagc ggacgcatca ctctggacgt ggttgaagat gagataaacc
     1381 gtctgcgcta taactggcag gagagccgcc cctccgcgct tacggcgttg ctgggcgcag
     1441 aggcagaaaa catcaatctc ttcgaccgta tgcaactgga acacgttatc gctatctgcc
     1501 gccaggcaaa gtcgctttct gcagccggac gacagctttt tgatgtttcg cgccagggca
     1561 aagccagcgt caatgacgcg gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt
     1621 gggaagccgt gcaggatcag cacagctcca gttgaatatg atggtccgtc agcacctgca
     1681 ttacgcttac tggcggcggg gcgtcggtgt agacggcatc taccatgctg atgctgccca
     1741 tattgaccat cgcgttacgg ccaaatttcg agtgatcgac aaccagcata acgtggcgcg
     1801 agttctcaat aatggcgcgc ttggtgcgaa cttcgtggta atcgaactcc agcagcgagc
     1861 cgtcgctatc gatgccgctt atccccagaa tgccgaaatc aaggcggaac tgggagataa
     1921 aatcgagcgt cgcttcgcca atgatcccgc catcgcggct gcgtaattcg ccaccggcga
     1981 gaataatgcg gaaatcttct tttaccatca acgtgttagc aacattgaga ttgttggtga
     2041 caatacgcaa attgctgtga ttaagcagcg cgtgcgccac cgcttccggc gtggtgccga
     2101 tatcgataaa cagtgtcgag ccgttgggga tttgctccgc cactttgcgg gcaatcctct
     2161 ctttttcttc ggtctgggtg gctttgcgat cgtgccacgg cgtgttaacc gaactggaag
     2221 gcagcgccgc accgccatgg tgacgcagga tcaggttttg ctccgccagc tcattgaggt
     2281 cgcggcgaat agtctgcggg ctgacggaga aatgctctac cagctcttca gtactgacat
     2341 aaccctgctg tttaaccagt tcgataatac cgttgtgacg ttgtgtttgt ttcatttata
     2401 aatccctgga attattttcg ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa
     2461 cccacggcta acccggcgat gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac
     2521 catccggcga caatccagat cagcgcaaag ataattaacc cacgttgcag gtaaatgcca
     2581 ctttgcggat cgcgttcacc acgtagccag acgtagccca tcagcgcata caccacgcca
     2641 gaaagcccgc caaaccacgg cccgctgaat ttttgctgca catagccgct taacagggcg
     2701 ctaatgagag taataacaat tagcttaccg ctaccgaggc gtttttccac cgcaccgccg
     2761 agataccacc accagagcag gttaaagagg atatgcatca gcgagaagtg cattaacgcg
     2821 tgggtgaagt aacgccagaa ctcaaatttc agtgctggat cgaatggcca ggccagccat
     2881 aacatcactt cctgatcgcc gagaatttgc atggcaataa acaccaccac gcaggcgatc
     2941 atcatcaccc aggtcaccgg acctgcgcgt tcacgcaagg cggcaaagaa aggataacgg
     3001 cgataatgca ggccactgcc ggtatggcct gcctgccagc tggccgccag ataacgcgga
     3061 tctgccgggt tttcgagaaa acgcgccagc tccgcccgta cgcgctcggc ctgggactca
     3121 tccgccagcc agacatcgct ttggttatgt tgttgaatcg tgaggataac accctgcgtc
     3181 gccatgtaat caacaaacgc ctgcgccacg cgggggttag caaaagaggt aatcatcaac
     3241 atcgttgctg tcgcttattc cacacaaaag gggacagtat aaagcgttac gcgccgtacg
     3301 ccacctctgc gggaaactga cgttgccagg cttcaaagcc gccgtcaatg ctatagacca
     3361 catcgtagcc ctgttgcagc agatactgcg ccgcgccttt gctgctattg ccgtgataac
     3421 acatcaccat caccggagtg tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt
     3481 tggttaaatg gaaagcctgc accgcatgcc ccatagcgaa actctgtgga tcgcgaatat
     3541 cgaccagcac cgcctctttt tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt
     3601 cgaactgatc catgcgtctc tctttcttta caaacaagtg ggcaaattta ccgcacagtt
     3661 tacgtcgaag cggcagataa acgccataat gttatacata tcactctaaa atgttttttc
     3721 aatgttacct aaagcgcgat tctttgctaa tatgttcgat aacgaacatt tatgagcttt
     3781 aacgaaagtg aatgagggca gcatggaaac caaagatctg attgtgatag gtggcggcat
     3841 caatggtgct ggtatcgcgg cagacgccgc tggacgcggt ttatccgtgc tgatgctgga
     3901 ggcgcaggat ctcgcttgcg cgacctcttc cgccagttca aaactcattc acggtggcct
     3961 gcgctacctt gagcactatg aattccgcct ggtcagcgag gcgctggctg aacgtgaagt
     4021 gctgctgaaa atggctccgc atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc
     4081 gcatctgcgc ccggcgtgga tgattcgcat tggtctgttt atgtacgatc atctgggtaa
     4141 acgcaccagc ctgccgggat caactggttt gcgttttggc gcaaattcag tgttaaaacc
     4201 ggaaattaag cgcggattcg aatattctga ctgttgggta gacgacgccc gtctggtact
     4261 cgccaacgcc cagatggtgg tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac
     4321 ctctgctcgc cgcgaaaacg gcctgtggat tgtggaagcg gaagatatcg ataccggcaa
     4381 aaaatatagc tggcaagcgc gcggcttggt taacgccacc ggcccgtggg tgaaacagtt
     4441 cttcgacgac aggatgcatc tgccttcgcc ttatggcatt cgcctgatca aaggcagcca
     4501 tattgtggtg ccgcgcgtgc atacccaaaa gcaggcctac attctgcaaa acgaagataa
     4561 acgcattgtg ttcgtgatcc cgtggatgga cgagttttcc atcatcggca ccaccgatgt
     4621 ggaatacaaa ggcgatccga aagcggtgaa gattgaagag agtgaaatca attacctgct
     4681 gaaagtgtat aacacgcact ttaaaaagca gttaagccgt gacgatatcg tctggaccta
     4741 ctccggtgtg cgtccgctgt gtgatgatga gtccgactcg ccgcaggcta ttacccgtga
     4801 ttacaccctt gatatccatg atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg
     4861 taagttgacc acctaccgca aactggcgga acatgcgctg gaaaaactga cgccgtatta
     4921 tcagggcatt ggcccggcat ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg
     4981 cgaccgcgac gattacgcag ctcgtctgcg ccgtcgctat ccattcctga ctgaatcgct
     5041 ggcgcgtcat tacgcacgca cttacggcag caacagcgag ctgctgctcg gcaatgcggg
     5101 ggcgataagc gacctcgggg aggatttcgg tcatgagttc tacgaagcgg aactgaaata
     5161 cctggtggat cacgaatggg tccgccgcac cgacgatgcc ctttggcgtc gcacaaaaca
     5221 aggcatgtgg ctgaatgcgg atcaacaatc tcgtgtgagt cagtggctgg tggaatatac
     5281 gcagcagaag ttgtcgctgg cgtcgtaagg atgatgcccg gttgaagtgc aaccgggcta
     5341 tttggattcc ttagactatt cattgtaaat aattgaataa cggcccctga gctaaaatcg
     5401 ccaaacttaa gcacgttacc caatcgcccc acttcttcct ctggtttcta ttttttctgc
     5461 accaatggca ataatatccc aatgcaaaac caagctgtgc actaatcggg ccgacaaaat
     5521 cagtccaagg ctgcggccaa aaaggggaaa aattcagggg aggccagaca aacgtcagaa
     5581 agaaatttcg gatattttcc agcaacgctg atttctctgg agaaatataa agaatgaaat
     5641 tcgagagaat taaccagaaa tagaccggcg aaaagaccag ccagcaatgc agtctgtttt
     5701 ttaattcagg aggcgttctg cgggagaatt gccaaccgat gccagccatt acactgaaaa
     5761 aaagtgcata ttcaaccatt aactcaagtt gtaattcctg aagagaaata cgcacaacca
     5821 ggagcggaaa ggagaatatt atttctgcca gaaaagttaa cgtgaatata agcacggcta
     5881 agacaataac cgtcggccat accaataatg gcacgataac aaaaccgaga attacagggg
     5941 gaatataaag ccggaattta atcattttta ttttcctggt gattcggatg atgcgtcata
     6001 catcgcgcat aaacactcat caattatcca gtcatcttcc cagacctgat attctgtcat
     6061 tggttcgggc tgttttagcg ggttctcaat cgcttgccgc gctctttctt tcagtaaagg
     6121 atgattattt ctagcccagg cattgatttg ggtggtaatg gcgttatcga tatcgtcctg
     6181 gttatctctg atataatcct cacaa
//
LOCUS       DAZPJP010000226         6149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPJP010000226 DAZPJP010000000
VERSION     DAZPJP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.229"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            79..855
                     /gene="exuR"
                     /locus_tag="ACHU9J_003845"
     CDS             79..855
                     /gene="exuR"
                     /locus_tag="ACHU9J_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1907282.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1095..1178
                     /gene="yqiM"
                     /locus_tag="ACHU9J_003846"
     CDS             1095..1178
                     /gene="yqiM"
                     /locus_tag="ACHU9J_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1907283.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1200..1862
                     /gene="yqjA"
                     /locus_tag="ACHU9J_003847"
     CDS             1200..1862
                     /gene="yqjA"
                     /locus_tag="ACHU9J_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1907284.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1866..2249
                     /gene="mzrA"
                     /locus_tag="ACHU9J_003848"
     CDS             1866..2249
                     /gene="mzrA"
                     /locus_tag="ACHU9J_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1907285.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2396..2764
                     /gene="yqjC"
                     /locus_tag="ACHU9J_003849"
     CDS             2396..2764
                     /gene="yqjC"
                     /locus_tag="ACHU9J_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1907286.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2802..3107
                     /locus_tag="ACHU9J_003850"
     CDS             2802..3107
                     /locus_tag="ACHU9J_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1907287.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3110..3514
                     /locus_tag="ACHU9J_003851"
     CDS             3110..3514
                     /locus_tag="ACHU9J_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1907288.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3504..3803
                     /locus_tag="ACHU9J_003852"
     CDS             3504..3803
                     /locus_tag="ACHU9J_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1907289.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3989..4381
                     /locus_tag="ACHU9J_003853"
     CDS             3989..4381
                     /locus_tag="ACHU9J_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1907290.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4451..5436
                     /gene="yqjG"
                     /locus_tag="ACHU9J_003854"
                     /pseudo
     CDS             4451..5436
                     /gene="yqjG"
                     /locus_tag="ACHU9J_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5729..6094
                     /locus_tag="ACHU9J_003855"
     CDS             5729..6094
                     /locus_tag="ACHU9J_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1907291.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1540 a         1514 c         1547 g         1548 t
ORIGIN      
        1 atttatatat gaccaatggc atacccatga ataaagtggt atgataactt cgtccgcatt
       61 ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca acaacttgcc
      121 gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa actgcctgcc
      181 gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga agccatcatc
      241 atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca tgtggtttcc
      301 aaccagccgc gccatcagca ggcggctgac aataatatgg aattcgccaa ttacggtccg
      361 tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt cgcggcaact
      421 caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc gcgcggcgaa
      481 caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc cctggcgacg
      541 cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccagcgtag ccataacccg
      601 tactggaaaa aactacacga acacattgat tcccgtaccg ttgataactg gtgtgatgac
      661 cacgatcaaa ttctcaaggc gttaattcgc aaagatcccc atgctgctaa gctggcaatg
      721 tggcagcatc tggaaaacac caaaatcatg ttatttaacg aaaccagcga cgatttcgag
      781 ttcaatgccg accgctatct attcgccgaa aacccggtag tgcatctcga tacggccacc
      841 agtggcagta aatgaaactc ctgtccgaca ggcgctgcga cgcgcctgtt cgcttcgtcg
      901 gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt
      961 cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg acctttgtta
     1021 caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg atttaacagg
     1081 gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac agcaataaca
     1141 ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag gaataatgaa
     1201 tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa accctggcca
     1261 atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc cttgaaaacg
     1321 gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc ggcgtgttga
     1381 ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt gccgccagcc
     1441 tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc accgtacaaa
     1501 actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt tttcataaac
     1561 acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca ctgctgccga
     1621 cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac tggatgagcg
     1681 gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa acgccgatat
     1741 ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg
     1801 tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt
     1861 aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc actgttttac
     1921 ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag tctacgctgg
     1981 cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc tggcatcacc
     2041 ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact ctgttaatta
     2101 catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga acattgcccc
     2161 atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg ctgacccggt
     2221 tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata tcactcactt
     2281 tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact accgcttcgt
     2341 tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt caagaatgaa
     2401 ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt atgccactac
     2461 cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg aaaaacacca
     2521 aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg ccaactgttc
     2581 agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag atgaggtggc
     2641 ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa
     2701 acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag cgcgcgacta
     2761 ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa gaacacacta
     2821 cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag gtgcttagct
     2881 catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg gagcaggcac
     2941 tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg
     3001 tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc gtgggaattg
     3061 gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac
     3121 tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat
     3181 catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa
     3241 agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg ctgcatttgg
     3301 tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc gcctgaatgc
     3361 gatgattgcc accaccgtgg tgttgctgct actggcactg attggcggta tctggacgtt
     3421 acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg caaacgatcg
     3481 gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc
     3541 aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt cgtgaatggc
     3601 tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg cgctcctggg
     3661 cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat atgctggtcc
     3721 gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa acgaccctca
     3781 agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc cttatccctc
     3841 aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca ccacgtttat
     3901 gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag
     3961 cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg gtgtactggt
     4021 agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa tcaccgctta
     4081 ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc tgccgctggt
     4141 gattctgctt gagttcggtg gtggtctggc aatcctgttc ggtttcctga ctcgcaccac
     4201 agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca gcaactttgc
     4261 tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg gattcctgct
     4321 gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata aaaagtggta
     4381 agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt tgctattgga
     4441 ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt acgataccaa
     4501 atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc tcactgccga
     4561 tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc gttatcatct
     4621 ctatatttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca aactcaaagg
     4681 actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa acggctggac
     4741 ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaacatg aatttctgta
     4801 tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc ccgtactgtg
     4861 ggacaaaaag aaccacacca tcgtcagcaa cgaatcaaca gaaatcatcc gcatgttcaa
     4921 taccgcgttt gatgcgctgg gcgcgaaagc gggtgattac tacccaccag ccctgcaaac
     4981 gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg tgtataaagc
     5041 tggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct
     5101 ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaacc agctaaccga
     5161 agccgatatt cgcctgtgga ctacgctggt gcgttttgat ccagtgtatg tgacccactt
     5221 caagtgtgat aagcaccgca tcagcgatta cctgaatctg tatggcttcc tgcgcgatat
     5281 ctaccagatg ccggggatcg ccgaaactgt caatttcgat cacatccgta atcattactt
     5341 ccgcagccat aagaccatca accctacggg gattatttca attggtccgt ggcaggatct
     5401 cgatgaaccg caggacgaga tgttcgcttc ggttaaaaaa aggcacctta cgaagtgcct
     5461 ctgataatta gctaagtgca tgttgttcat atcggatgcg gcgtgaacgc cttatcaaac
     5521 ctacaaaaca tttatattca atatgttgca atgaccgcgc aggccagata agcgtagcgc
     5581 atctggcgat tttgcgcttg tcatcagtct tagcgcgcct tcacagcact ttattaattc
     5641 cttgaacgaa tatttactgc catttcattc acgtctattc ttaatttgct gctttaaaaa
     5701 caagtgattg agcaaaattg aggcaaaaat ggactggtat ctgaaagtaa taaaaaatta
     5761 tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg atgtttattc tggtcaacat
     5821 catctttacg ttcgtgctgg ggctgctgga taaaatgtta ggctggcaac gcgctggtgg
     5881 cgaaggcatc ctgacgacaa tctacggtat tctggtgttt ttaccgtggt gggcagttca
     5941 gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg gcactgctat tcttaatccc
     6001 gtttatcggc tggcttatta tcatcgtctt taactgccag gcgggtacgc caggcgaaaa
     6061 ccgctttgga cctgatccaa aactggaacc ttaaaacaaa tctgctcgcc ggatgtctga
     6121 ctcatccggc ttttatcatc atgttgatg
//
LOCUS       DAZPJP010000227         6121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPJP010000227 DAZPJP010000000
VERSION     DAZPJP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.230"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHU9J_003856"
     CDS             <1..358
                     /locus_tag="ACHU9J_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1907292.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHU9J_003857"
     CDS             complement(398..1069)
                     /locus_tag="ACHU9J_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1907293.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHU9J_003858"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHU9J_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1907294.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHU9J_003859"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHU9J_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1907295.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHU9J_003860"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHU9J_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1907296.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHU9J_003861"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHU9J_003861"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1907297.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHU9J_003862"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHU9J_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1907298.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHU9J_003863"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHU9J_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1907299.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHU9J_003864"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHU9J_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1907300.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAZPJP010000228         6094 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPJP010000228 DAZPJP010000000
VERSION     DAZPJP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.231"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(193..384)
                     /locus_tag="ACHU9J_003865"
     CDS             complement(193..384)
                     /locus_tag="ACHU9J_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907301.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(381..803)
                     /locus_tag="ACHU9J_003866"
     CDS             complement(381..803)
                     /locus_tag="ACHU9J_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HFR1907302.1"
                     /translation="MYCTVKEVIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(850..1275)
                     /locus_tag="ACHU9J_003867"
     CDS             complement(850..1275)
                     /locus_tag="ACHU9J_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR1907303.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     KYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(1688..2458)
                     /locus_tag="ACHU9J_003868"
     CDS             complement(1688..2458)
                     /locus_tag="ACHU9J_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907304.1"
                     /translation="MNKTLNALVYRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSECVEHILSSGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMVDALAKIRG"
     gene            complement(2503..3022)
                     /locus_tag="ACHU9J_003869"
                     /pseudo
     CDS             complement(2503..3022)
                     /locus_tag="ACHU9J_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2952..3173)
                     /locus_tag="ACHU9J_003870"
     CDS             complement(2952..3173)
                     /locus_tag="ACHU9J_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907305.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(3174..3857)
                     /locus_tag="ACHU9J_003871"
     CDS             complement(3174..3857)
                     /locus_tag="ACHU9J_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR1907306.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(3934..4227)
                     /locus_tag="ACHU9J_003872"
     CDS             complement(3934..4227)
                     /locus_tag="ACHU9J_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907307.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASREPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
     gene            4375..5337
                     /gene="parM"
                     /locus_tag="ACHU9J_003873"
                     /gene_synonym="stbA"
     CDS             4375..5337
                     /gene="parM"
                     /locus_tag="ACHU9J_003873"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1907308.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKIILNGGDTFTIKDVKVMPESIPAGYEALQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            5340..5690
                     /locus_tag="ACHU9J_003874"
     CDS             5340..5690
                     /locus_tag="ACHU9J_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1907309.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRAPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            5949..6068
                     /locus_tag="ACHU9J_003875"
                     /pseudo
     CDS             5949..6068
                     /locus_tag="ACHU9J_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1568 a         1674 c         1513 g         1339 t
ORIGIN      
        1 agataaagga acaacaacaa catcaggaac agttccataa cataagaaag aaacactcct
       61 gagtcagaca catcacagac gggaagggta acgggcgacg cgccagcgtg gcccgcttcc
      121 cttccggcag gcgtcagcct gccaggggtt ttcgcgcggg ctgacaggcg gcctccaccg
      181 cctgcccctg tatcacctgt ttctgctgcc ctgtaagatg tagtgaccgc ccaggaaaac
      241 cgcctgctga ttcgccacaa aatcgcagat ttcatcaaca atctgcgcgg tggtgagcgg
      301 tttctgcccc atttcacgac gccgcgcatt aatcacggcc ctgtaattac gcagaatttc
      361 gcgggtttct gtgctgatat tcatgacatc gtctccccgt ccagcgcctg caacacaacg
      421 tccattgctt cgcgttcctc cctgacgaat gcgtcaccgt cgcccccgtt ctcactcccg
      481 acggcataca ggcgcttcat ttcactgcct gccagcgcca tcactttttc cagcatcacc
      541 gccgggactg tcacatgacg gctggcgcgc ttttcttcca tcagttcacg aacaacgtcg
      601 tgaacaacgc tgacatccgc accatgctca aaggcatcgt ccagtcgctg catgacggtt
      661 tccagctcat cgtctgacag gttccagtcc tgggctatgt gacgcacatc cccacgggtc
      721 agcaccacgg caaaaacgca ttcactgtcc ggcacgtctg tatccagtac atcgcggatg
      781 acttctttaa cagtacagta catgttctcg tctcctttac aggggcggca ttgccgcccc
      841 gtccgttaat cagtccagtg ccgcgtaaat cgcgttgcat tcggggtgaa attcgatgtg
      901 gctccacaac tgcgcatcgc gcatcctgta caggtgtgtc aaccctgcat ccccgctgtc
      961 gtggtacagc cacaactggc ggttaatcac aagggaggta aggatgatgc ctgcggcatc
     1021 agcactcacg gtacggtcaa accagtttgc accgttcacc atgtggaaac ggtcgccatc
     1081 cggcatcatg taaccgccac cgtccggcag gcggatgaag tgccagaatc cgccttcata
     1141 tttcggcatc atttttttcg ccagcgcgta tacagacgct tcggcataca gaaaatccag
     1201 gccaaacagg taaggcagaa aggagagacg gtcgcactct tcaacattca gagtgacagg
     1261 tttcgcatat tgcatgatgt tttcctccgt tgttcacata ttccgcgaca ccgggcggtg
     1321 ccgtgcctcc atctttgccg ggcctgcagg gttgcaaggg cgcaagggaa gccgcagcct
     1381 gacgcgcacg gctgacggga gttggagggt gagaccgcga aatctgagcg ggtgaacgac
     1441 cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggttc accttacctt
     1501 gcaacccgcc cgcagaagcc cggctacgat ggaggcacgg cacagcccgc acacgacgct
     1561 gaggccacat aactgaaaat ccacaaggcc atgccccgca tggccgggta aagcggagcc
     1621 tgcggcgaca tccttttgct ctttccgggg accggcagaa aaaaagcggg gattttcccc
     1681 gccatcatca gcccctgatt tttgccagag catccaccat gctgcggatt tcatcccgtg
     1741 cctccagaac aacctcccgc agaatatccc ggtcaaacca gcgacgggac ggttcatccg
     1801 gtatacgcag cacatcacag accagaccac tggacaggat atgctccaca cactcactga
     1861 ccagatacat ttccaccgcc gaaaaacgtg ctccccggtt caggatagca tcgagcacca
     1921 cgggcagatt ttcgtcatct tcatcaagcc agcaggggta atcgctttcc ttcacaacca
     1981 gacgaaagcg gcgcgtctgg cgggtcagca acgtctgacc ggtggtggtc agcgccgcac
     2041 ggatacgccg cgcatcttct gccacccggc aactgacact gcatactgca tcgcgcaccg
     2101 catcaagaac cgccctgatt tcttcctccg tcagccattc accggcatac gggaaagaca
     2161 cctgcgttcc gtctgccgga agtaccggca gcgactgcca ctgactgccg gacagaacaa
     2221 gttccgctcc ggttccggtc agtttctgaa tggcagaggc caggagcggc ggcagtacgc
     2281 cgccgtgacg gttgataagt aacgtcgcca gccggggcgc atccagccgc acgtaaatgc
     2341 tgatccagcc cggatagtgc ggattcagtt gttcaatatc cgtctcttcc ggccagccct
     2401 gcgccagcag caggttacgg gcgtggcgat aaaccagtgc attcagcgtt ttgttcatga
     2461 ttaatggtct ccctgaatcg ccgcccggag gcggcgataa catcagtcgt tttcgtgtgt
     2521 gttgtttttt cagcagggcg cgggcctgat taaccaccgc ctgacggcgg gtgacacatt
     2581 ccgggacggc gaggccatga tcccgggcgg cccattcacg tttccacagt gcctgttcgg
     2641 cggtctgaat caggaacgcc agcagttcag ccgggacggt cacctggcgg ttttctctca
     2701 cgttattgat gatctccatc gccacgccgg aagaaatacc ggattcagtc cgctggtctt
     2761 ccgggatgtc ctccaggcgc gccaggacag tcctgatttc atgatcggac agggaaatat
     2821 ccattccgtc agcgagggcc tgaatttctt ccggggacca gataaccagc atcagcggcg
     2881 tgtctcccgg atactgcgct gccagctccc ggcatagcgt ttcgcatgtt ccgtacattc
     2941 tcgtctcctt ctcaatcttt gaagcaggaa tccagttcca gaatttcggt atacgcctcc
     3001 atgaacaggc gattggcgcg atacagcgtc tggcgtgtca ggcgttcaac aagcacatcg
     3061 gaatcaaagc gacagcgatg ctccatatcg ttcagcctgg cccgaaggtc acacatggtg
     3121 ttggccacct ccgccacgtc ggcgcgaagt ttttcgtgtc cggcatagtt catttacgca
     3181 gcctccggtt caaaccagtt atcattcgcg gccccctgct gcatggcccg ttgcacggcg
     3241 gccagtcgtt gctgtccggc acggtgatac tgttcaagca gctcgatacc gatataccgg
     3301 cgtccggact gaagggcggc gacgcaggtt gagccgctgc ctgcaaacgg gtccagcaca
     3361 atcgcgttcg ggtgtgtgaa gctctcaatc agcggttgca ggctggtaac gggcttttca
     3421 gtcgggtgat gacgattgcc tgaatatttc cagcccagca cgtcgggcaa tggcttctga
     3481 ggcagcgccg gacgtccttt tgccaggatg taggcgcatt cgtggcgata gcccacatat
     3541 gcggccttcg atgtgtaggt tttggtaaac accaggtggc caaccacgct gaatcccgca
     3601 tttttccagg cggccataaa gcgatcgacg cggttccagc catagaagct caccattaac
     3661 gcgtcttttt tgagtacgcg gtacatttca ttacaggctg gttgcagcca ttcatcggtt
     3721 ttatcgccgg cgatggtgcg cccggaacgg tcacggaaac cgacgagata cggcgggtcg
     3781 gtgaggatga aatcgacagc attgcccgga aacgtggcca taacacgcac gcagtcaccc
     3841 aggataaaac gggacataac agcactcctt ctcttacaga gggcaccggg agggcacccc
     3901 tgtggtgccc gttccccgct gcctgtgggc tgttcaccgc gtccggtaac gggtcaggcc
     3961 gaccgtggaa taccgcagcc gtcgtctttc tgacggcaag gctatgccgc gaaagcggct
     4021 tgaagggcgt tcatcagaaa agggctcccg gctggcgtac actgtcacca acgccagccg
     4081 ggagcggtaa aagcgcccgt cccgtgcccg gccggacgcg ggacactgac cacaggcagc
     4141 gttcctgccg cgccgcgtca gccggcgcag taaaaagagg agcccggcga catcttttgt
     4201 cacccgccaa acaaaaccca aaaacaaccc acacccaacc caataaaaca ccaaaacaag
     4261 acaaataatc attgattgat ggttaaaatg gggtaaactt gacaaataaa cccacttaaa
     4321 acccaaaaca tacccaaata cacaccaaaa aaaacaccat aaggagtttt ataaatgttg
     4381 gtattcattg atgacggttc aacaaacatc aaactacagt ggcaggaaag cgacggaaca
     4441 attaaacagc acattagccc gaacagcttc aaacgcgagt gggcagtctc ttttggtgat
     4501 aaaaaggtct ttaactacac actgaacggc gaacagtatt catttgatcc aatcagcccg
     4561 gatgctgtag tcacaaccaa tatcgcatgg caatacagcg acgttaatgt cgttgcggtg
     4621 catcacgcct tactgaccag tggtctgccg gtaagcgaag tggatattgt ttgcacactt
     4681 cctctgacag agtattacga caggaataac caacccaata cggaaaatat tgagcgtaag
     4741 aaagcaaact tccggaaaaa aattatatta aatggtgggg atacattcac aataaaagat
     4801 gtaaaagtca tgcctgaatc tataccagca ggttatgaag ctctacaaga actggatgag
     4861 ttagattctt tattaattat agacctcggg ggcaccacat tagatatttc tcaggtaatg
     4921 gggaaattat cagggatcag taaaatatac ggagactcat ctcttggtgt atctctggtc
     4981 acatctgcag taaaagatgc actttctctt gcgagaacaa aaggaagtag ctatcttgct
     5041 gacgatataa ttattcacag aaaagacaac aactatctga agcaacgaat taatgatgag
     5101 aacaaaatat caatagtcac cgaagcaatg aatgaagcac ttcgtaaact tgagcaacgt
     5161 gtattaaata cgctcaatga attttctggt tatacgcatg ttatggttat aggcggtggc
     5221 gcagaattaa tatgcgatgc agtaaaaaaa cacacacaga ttcgtgatga acgttttttc
     5281 aaaaccaata actctcaata tgatttagtt aacggtatgt atctcatagg taattaataa
     5341 tggacaagcg cagaaccatt gccttcaaac taaatccaga tgtaaatcaa acagataaaa
     5401 ttgtttgtga tacactggac agtatcccgc aaggggaacg cagccgcctt aaccgggccg
     5461 cactgacggc aggtctggcc ttatacagac aagatcccag ggcccctttc cttttatgtg
     5521 aactgctgac gaaagaaacc acattttcag atatcgtgaa tatattgaga tcgctatttc
     5581 caaaagagat ggccgatttt aattcttcaa tagtcactca atcctcttca caacaagagc
     5641 aaaaaagtga tgaagagacc aaaaaaaatg cgatgaagct aataaattaa ttcaattatt
     5701 attgagttcc ctttatccac tatcaggctg gacaaaggga acttaatcaa gttatttcat
     5761 tagcaatgat tacataaaat aaatacagtg tcgttattat gaaattattt cacggcactt
     5821 ataaaaaaaa cagtaccaat aatcaaacct ggtgagttcg caatgtctgg caataacgtt
     5881 tttgatgggt tgttttccag cccggagcgc gatattgcca ccagtcacag aaacaccgag
     5941 aacaccagat ttcagcggtc gctgaatttg aacgagatct tttacttgaa cgaacttatt
     6001 cagggatagc aagagcacgg gatgcaggaa aacgttttgg tcgcccggta atgctgccaa
     6061 cttactgatt tagtgtatga tggtgatttt aagg
//
LOCUS       DAZPJP010000229         6087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPJP010000229 DAZPJP010000000
VERSION     DAZPJP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.232"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHU9J_003876"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHU9J_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1907310.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHU9J_003877"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHU9J_003877"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1907311.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHU9J_003878"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHU9J_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1907312.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHU9J_003879"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHU9J_003879"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1907313.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1616 a         1609 c         1450 g         1412 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcgg
//
LOCUS       DAZPJP010000230         6003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPJP010000230 DAZPJP010000000
VERSION     DAZPJP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.233"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(116..478)
                     /locus_tag="ACHU9J_003880"
     CDS             complement(116..478)
                     /locus_tag="ACHU9J_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1907314.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            1015..2928
                     /gene="mtlA"
                     /locus_tag="ACHU9J_003881"
     CDS             1015..2928
                     /gene="mtlA"
                     /locus_tag="ACHU9J_003881"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1907315.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3158..4306
                     /gene="mtlD"
                     /locus_tag="ACHU9J_003882"
     CDS             3158..4306
                     /gene="mtlD"
                     /locus_tag="ACHU9J_003882"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1907316.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4306..4893
                     /gene="mtlR"
                     /locus_tag="ACHU9J_003883"
     CDS             4306..4893
                     /gene="mtlR"
                     /locus_tag="ACHU9J_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1907317.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4905..5114)
                     /gene="yibT"
                     /locus_tag="ACHU9J_003884"
     CDS             complement(4905..5114)
                     /gene="yibT"
                     /locus_tag="ACHU9J_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1907318.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5399..5761
                     /locus_tag="ACHU9J_003885"
     CDS             5399..5761
                     /locus_tag="ACHU9J_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1907319.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1524 a         1477 c         1547 g         1455 t
ORIGIN      
        1 cccggttgat cgaatggatg cgaatcgctg atcgaacttc gatgccaatc cgtgatcgaa
       61 tagagcggaa tacgcaccag caccacgtaa gttaaaacaa tcaatagatc cataattaga
      121 tctccgggaa cgtggaatag tcagtctttt tctcggcggc gagttgatgc tctagccggg
      181 aaattcggtc agaaagtgcg gcgatttccg ggtcagtcgc tttctcctgc gacgcaacat
      241 gtgactgcat gccccacccg cgctccggtt gatagagcgt cgcccagatc cacaggaacg
      301 gccagataac atgcagggta aacaggctca cccagcccgc cgtatgaata gcgtcggcat
      361 ggggatggtt gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat
      421 aaaaaatcac cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat
      481 ctgcctcgga ttcacgttta tcagtgttgt ttttgggctg gcagccagaa gggagtcagg
      541 ctgatatttt gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt
      601 tagaggtgat gagttgctta gttacataac aattgtatga cgaaggcata acatgctgta
      661 gatcacatca ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac
      721 aaaccatcaa aacaaaaatg tgacactact cacatttaaa tgccattttt agcgaaaatc
      781 gccgccttgt tgctttttta cacaagcgtt ttgtgatgaa cgtcacgtta attacctctc
      841 taccccctat atttatgtga ttgatatcac acaaaaggcc gtcgactgga cagttaaccg
      901 attcagtgcc agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca
      961 aaaaacctcg ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca
     1021 tccgatatta agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca
     1081 aatatcggcg cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg
     1141 ttaccgaacg agacgctggc gaagctggtc gggccgatga tcacttatct cctgccgctg
     1201 ctgatcggtt ataccggtgg taagctggta ggcggcgaac gtggcggcgt agtcggtgcc
     1261 atcaccacca tgggcgttat cgtcggcgca gacatgccga tgttcctcgg ttctatgatt
     1321 gcaggtccgc tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc
     1381 aaatccggtt ttgagatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc
     1441 gctattctgg cattcctcgg cattggcccg attgttgaag ccctgtccaa aatgctggct
     1501 gcgggcgtta acttcatggt tgtccatgac atgctgccgc tggcgtctat ctttgttgaa
     1561 ccggcgaaaa tcctgttcct caacaacgcc attaaccacg gtatcttctc gccgctgggt
     1621 attcagcagt cccatgaact gggtaaatca atcttcttcc tgattgaagc taacccaggt
     1681 ccaggtatgg gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct
     1741 gcgggcggtg cggcaatcat ccacttcctg ggtggtatcc acgaaatcta cttcccgtat
     1801 gtgctgatga atccgcgtct gatcctcgca gtcatcctcg gcggtatgac tggcgtgttc
     1861 acgctgacta tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct
     1921 gtactggcga tgacaccaaa aggtgcttat ttcgctaaca tcgcgggtgt gtgtgcggcg
     1981 atggctgtct ccttcgttgt ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa
     2041 gatgatattg aagcagcaac tcgtcgtatg caggacatga aagctgagtc taaaggcaca
     2101 tctccgctgt ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt
     2161 gcctgtgacg ccggtatggg ttccagtgcg atgggcgcag gcgtgctgcg taagaaaatt
     2221 caggatgcag gtctgtcaca gatttcggtc actaacagcg cgatcaacaa cctgccgcca
     2281 gatgtggacc tcgtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg
     2341 caggcgcagc atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctaacc
     2401 gaacgtctgg ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg
     2461 aaagacagct ttgacgattc cagtgctaac ctgttcaaac tgggcgcgga gaacatcttc
     2521 ctcggtcgca aagcagcaac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg
     2581 aaaggcggtt acgttgagcc ggaatacgtt caggcgatgc tggatcgtga aaaactgacc
     2641 ccgacttatc tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc
     2701 gtactgaaaa cgggcgtggt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa
     2761 gaagatgaca ttgcccgtct ggtgattggt attgcagccc gtaacaacga gcacattcag
     2821 gttatcacca gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac
     2881 accaccagcg tggatgaagt gctggaactg ttggcaggtc gtaagtaatc caatcccacc
     2941 ctctccacat ggagaaggtg gggttaattg cctgatgcgc tacgcttatc aggcctacag
     3001 gatgcatcac gatttgttga atttgcacgt tcttgtaggc cggataaggc gcttacgccg
     3061 catccggcgc tgcccctctc ctcacggaga gggtttgggt gagggaaaag cctcacccca
     3121 gccctctcgg gtaaaaacat tgatgaaggt taatactatg aaagcattac attttggcgc
     3181 aggtaatatc ggtcgtggct ttatcggtaa actgctggca gacgcgggta tccaactgac
     3241 gtttgccgat gtcaatcagg tggtacttga tgccctgaat gcccgtcata gctatcaggt
     3301 tcatgtggtt ggtgaaaccg agcaggtaga taccgtttcc ggcgtcaatg ctgtcagcag
     3361 cattggtgat gatgtcgttg atctgattgc tcaggtcgat ttagtcacta ccgccgttgg
     3421 cccggttgtg ctggaacgta ttgctccggc tatcgccaaa gggctggtga aacgcaaaga
     3481 acaaggtaat gaatccccgc tgaacatcat cgcctgtgaa aacatggtac gcggtaccac
     3541 gcagctgaaa ggccatgtga tgaacgccct gccggaagac gccaaagcgt gggtagaaga
     3601 acacgttggc tttgtcgatt ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac
     3661 taacgatccg ctggaagtga cggtagaaac tttcagcgaa tggattgtcg ataaaacgca
     3721 gttcaaaggc gcactgccga acatcccagg catggagtta accgacaacc tgatggcatt
     3781 tgtcgaacgt aaactcttca ccctgaacac gggtcatgct ataaccgcgt acctcggaaa
     3841 actggccggt catcagacca ttcgtgacgc tattctcgac gagaaaatcc gcgcggtggt
     3901 aaaaggtgcg atggaagaaa gtggtgcggt actgatcaag cgctacggct ttgacgcaga
     3961 caaacatgcg gcgtacatcc agaaaatcct cggtcgtttt gagaacccgt atctgaaaga
     4021 tgatgtagag cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg accgtctgat
     4081 caagccattg ctcggtacgc tggaatacag ccttccgcac aagaatctga ttcaggggat
     4141 tgctggtgca atgcacttcc gcagtgaaga tgatccgcag gctcaggaac tggcagcact
     4201 gatcgctgac aaaggtccgc aggcggcgct ggcacagatt tccgatcttg atgccaacag
     4261 cgaggttgta tccgaggcgg taaccgctta taaagcaatg caataatggt ggaccaggcg
     4321 caggacaccc tgcgcccgaa taacagattg tcagatatgc aggcaacaat ggaacaaacc
     4381 caggcctttg aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt
     4441 ctgatcaccg ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct tcaggtattc
     4501 cgcaaagacg attacgcggt gaagtatgct gtagaaccgt tactcgacgg cgacggtccg
     4561 ctgggcgatc tttctgtgcg tttaaaactc atttacgggt tgggcgtcat taaccgccag
     4621 gaatacgaag atgcggagct gctgatggca ttgcgtgaag aactaaatca cgacggcaac
     4681 gagtacgcct ttaccgacga cgagatcctt ggaccctttg gtgaactgca ctgcgtggcg
     4741 gcgttaccac cgccgccaca gtttgaacca gcagactcca gtttgtatgc aatgcaaatt
     4801 cagcgctatc aacaggctgt gcgatcaaca atggtccttt cactgactga gctgatttcc
     4861 agaatcagct taaaaaaagc ctttcaaaag taagcaacgt ctgcttactg cccctctacc
     4921 tgcttcggcc gataaagccg acgataatac tccagacgtt gtagatataa cggcacgctt
     4981 tcatcgggta ttccggacgg aatcgcgtta cggggaggaa gttttttcag atactcccgg
     5041 aacgcctggc ttgatgccat gaaatctacg gctttatcga taagaagcgg aacgttttca
     5101 cctaatttgc ccatgatgat atctccgtat taccccgcgc cgggaatgcg cggccgccaa
     5161 ttttagttta ggttcccggt aaagatgaca ttaaggaaaa cgtgctgaat cctcaataat
     5221 catccgctgc tgtttattat cttttttcta tagatttctt aattaatcaa taaattgcgt
     5281 agtttttaag catgttaatg attacgttac agaactattg caatgtcatc agttgtgaac
     5341 aaagcacctg gtcgcgcata ctaggggcta taaatttatc tttatcagaa gccatcacat
     5401 gaaagaagtc gaaaaaaacg aaatcaaacg tctcagcgat cgtctggacg ccatccgcca
     5461 ccagcaggcc gatctgtcgc tggttgaagc cgcagacaaa tatgccgagc tggaaaaaga
     5521 gaaagccacg ctggaagcag aaattgctcg cctgcgtgaa gttcatagcc aaaaactgag
     5581 taaagaagca cagaagctga tgaagatgcc gtttcagcgc gcaattacca aaaaagagca
     5641 ggctgatatg ggcaagctga agaaaagtgt tcgcggactg gtggttgtgc acccaatgac
     5701 cgcgctgggc cgtgaaatgg gcctgcagga gatgactgga ttctctaaga ctgcgtttta
     5761 agcgcctcag acgactggtt cgatcatatc aacccgcatc tggcttgcct tagctggtca
     5821 atgcggggac tctcctgtct actcgcctaa ttaccaaaca attcactccc tttacgtcga
     5881 tttaccgctg gttgatattc tgaaaatgca tttttaccga aatagccacc tataaacaat
     5941 aaaaatatat agaagatatc atttattaat taacaagcag ttacgtctct ttttttcgga
     6001 ttt
//
LOCUS       DAZPJP010000231         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPJP010000231 DAZPJP010000000
VERSION     DAZPJP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.234"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHU9J_003886"
     tRNA            292..368
                     /locus_tag="ACHU9J_003886"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHU9J_003887"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHU9J_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1907320.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHU9J_003888"
     CDS             complement(1357..2247)
                     /locus_tag="ACHU9J_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1907321.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHU9J_003889"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHU9J_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR1907322.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHU9J_003890"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHU9J_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR1907323.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPJP010000232         5843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPJP010000232 DAZPJP010000000
VERSION     DAZPJP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.235"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            179..802
                     /gene="nfeR"
                     /locus_tag="ACHU9J_003891"
                     /gene_synonym="yqjI"
     CDS             179..802
                     /gene="nfeR"
                     /locus_tag="ACHU9J_003891"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1907324.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(909..2429)
                     /gene="aer"
                     /locus_tag="ACHU9J_003892"
     CDS             complement(909..2429)
                     /gene="aer"
                     /locus_tag="ACHU9J_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1907325.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2847..4226
                     /gene="ygjG"
                     /locus_tag="ACHU9J_003893"
     CDS             2847..4226
                     /gene="ygjG"
                     /locus_tag="ACHU9J_003893"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1907326.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4268..4600)
                     /locus_tag="ACHU9J_003894"
     CDS             complement(4268..4600)
                     /locus_tag="ACHU9J_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1907327.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4819..5802
                     /gene="ebgR"
                     /locus_tag="ACHU9J_003895"
     CDS             4819..5802
                     /gene="ebgR"
                     /locus_tag="ACHU9J_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1907328.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1425 a         1582 c         1466 g         1370 t
ORIGIN      
        1 gtctgataag acgaaccgcc tcttctcagg catcattact caacgccgga tgcggcgtga
       61 acgccttatc cggcctacgt gtgagatgag tccacattta aaaatcattt ttattcttgc
      121 aagagtgctc atatcagata taaattagat atatctaatc aagcaaaagg aggctgatat
      181 gagccatcat cacgaagggt gttgtaaaca tgaaggccag ccacgccatg agggctgctg
      241 caaaggtgag aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca
      301 atgctgcggt ggtcgccacg gtcgcggcgg cggtcgtcgg caacgtttct ttggtcacgg
      361 tgaattgcgt ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgaatt
      421 gattaaagcg attgagaatc taacccaggg gaattacacc ccaagcccgg gcgtcatcta
      481 cccaacgctg gattttctgc aggagcagtc gctgattacc atccgcgaag aggaaggtgg
      541 taagaagcag attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca
      601 ggtggagatg attgaagaac gcatcaaagc gcgttgcgtt ggtgctgcgc tgcgccagaa
      661 cccgcaaatg aagcgggcgc tggataattt taaagcggtg ctggatttac gcgtcaacca
      721 gagcgatatc actgatgcac aaataaaaaa gatcattgcg gtgatcgacc acgccgcttt
      781 tgatattacc caactggatt aatcaccgat tgccggatgc ggcgtaccct acacaccccg
      841 caggcctgat aagatgcgcc agcatcgcat caggcattgt gctccaaccg ccggatccgg
      901 cataccgatt aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg gtgtttcacc
      961 atcgccgaca cctgcgcact ctcttccacc agctcagcat ttttctgggt gatcaggtta
     1021 agctcatcca ctgcacgggt caggctggaa agcccatcgg cctgttccag cgttgaatgg
     1081 ctaatctggg cgatcaactg ggtgacgttt ttcacctgtg ccacaatatc ttccatcgtc
     1141 cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct tatcagcact ggcatcaatc
     1201 agcttgcgaa tatcgttggc agcattagcg ctgcggctgg ctaaatgacg cacttcccct
     1261 gccaccactg caaaaccttt gccctgttcg ccggcacgcg ccgcttccac cgccgcattc
     1321 agggccagaa tattggtctg aaacgcaata tcgttaatca gcgaagtaat ggtgccaatg
     1381 cgctgggtac tgtcggcgat atcgtccatt gtcttgatca ccgtggtcat cgcctcccca
     1441 ccctgcactg ccgcattact ggcggtgata gaaagtttat cggcagccga cgccgtggca
     1501 gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg ctgaacgtta
     1561 tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag cgtctcactg
     1621 ccatttctga cactggacac ctggcttgag acatcgttaa ttagccaacg gcacatcagg
     1681 ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt caggtgctca
     1741 acactattac gctctccggt cgccaccttc agtgcctgac gggcgacatt ttctatcggg
     1801 cgcacaatct gccattcaaa acaagcgctt gccaacaata ccactaacac acagaggaaa
     1861 tacgtcacca ccggggcagc aacaaaccaa agcatggccg ccagcaagat aaacatcagg
     1921 gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc cagccagcct
     1981 ttacgcacca ccaggccttt atgaatacgc ttactggtac gtccggcgtt cagcgctttg
     2041 tacagcggct ccaccgccgc aatctcttca tccgtcgccc gggtacgaat cgacatatag
     2101 ccactgattt ttccctcgcg caccatcggt accgcattgg cccgcaccca ataatggtcg
     2161 ccatttttgc ggcgattttt cacgatgccg ctccagggct cccctttttt cagggtgaac
     2221 cacatatccg caaacgccgc ttttggcata tccgggtgac gcaccatgtt gtgcggctgc
     2281 ccttgtaact cttgcaaggt aaagccgctc acctgcacaa aagtgtcatt agcatgagtg
     2341 atatagcttt gcagatcggt agtggacatc agagtggtat cgtccgccag cggggtattt
     2401 tgctgggtga catacggatg agaagacatg ttcgcgtcct gtgcaggtta tatggttgtt
     2461 aacttcttgt cagagtttat gtcggccccg ctgcggttat ctttaaccga ttaatttgat
     2521 ttagatcgca atttgcgatt aaagcacaaa tctaattctt tgatttaaaa tactttcact
     2581 ctgttactat acgaaaacgt taattatctt gcccaaaaat caggcaatta ttgccctgaa
     2641 aacgtgcatt tgcgcagcaa tcatcaaatc catacccgac aaaaaccgtg caaaataaca
     2701 acaaatgtta acagatagca ttaaatattg cacaaatgat aaccgaattt gtgtttatcc
     2761 cgattttcgc gatcgcagcc ggagtggcgc aatccctgca atacttaaat cggtatcatg
     2821 tgatacgcga gcctccggag catattttga acaggttacc ttcgagcgca tcggctttag
     2881 cgtgcagcgc ccacgccctg aatctcattg agaagcgaac gctggatcat gaggagatga
     2941 aagcacttaa ccgagaggtg attgaatact tcaaagagca tgtcaatccg gggtttttag
     3001 agtatcgcaa atctgttacc gccggcgggg attacggagc cgtagagtgg caagcgggaa
     3061 gtttaaatac gcttgtcgac acccagggac aggagtttat cgactgcctg ggaggttttg
     3121 gaattttcaa cgtggggcac cgtaatccag ttgtggtttc cgccgtacag aatcaacttg
     3181 cgaaacaacc gctgcacagc caggagctgc tcgatccgtt acgggcgatg ttggcgaaaa
     3241 cccttgctgc gctaacgccc ggtaaactga aatacagctt cttctgtaat agcggcaccg
     3301 agtccgttga agcagcgctg aagctggcga aagcttacca gtcaccgcgc ggcaagttta
     3361 cttttattgc caccagcggc gcgttccacg gtaaatcact tggcgcgctg tcggccacgg
     3421 cgaaatcgac cttccgcaaa ccgtttatgc cgttactgcc gggcttccgt catgtgccgt
     3481 ttggcaatat cgaagccatg cgcacggctc ttaacgagtg caaaaaaacc ggtgatgatg
     3541 tggctgcggt gatcctcgaa ccgattcagg gtgaaggtgg cgtaattctg ccgccgccgg
     3601 gctatctcac cgccgtacgt aagctatgcg atgagttcgg cgcactgatg atcctcgatg
     3661 aagtacaaac gggcatgggg cgcacgggca agatgttcgc ctgcgagcat gagaacgtac
     3721 agccggatat cctctgcctt gccaaagcgc tcggcggcgg cgtgatgccg attggcgcga
     3781 ccatcgccac tgaagaggtg ttttcagttc tgttcgacaa cccattcctg cataccacca
     3841 cctttggcgg caacccgctg gcctgtgcgg cggcgctggc gaccatcaat gtgttgctgg
     3901 agcagaactt accggctcag gctgatcaaa aaggcgatat gttgctggac ggtttccgtc
     3961 aactggcgcg ggaatatccc gatctggtac aggaagcgcg tggtaaaggg atgttgatgg
     4021 cgattgagtt tgttgataac gaaatcggct ataactttgc cagcgagatg ttccgccagc
     4081 gcgtactggt ggccggaacg ctcaataacg ccaaaacgat ccgcattgaa ccgccactga
     4141 cactgaccat tgaacagtgt gaactggtga tcaaagcggc gcgtaaggcg ctggcggcca
     4201 tgcgagtaag tgtcgaagaa gcgtaatatc aaatcggatg gcgatacgac gtcgccatcc
     4261 gatcttttta tacgacgcgt acgcccgcag gcatcatccg ctccggcgtt aacagcacgc
     4321 tttcactgcc gtcatcggtt tccgcacaca acagcatgca ttccgacgtt tcaccacgca
     4381 ttttcgcttt ttgcagattg cataagacca ccaccgtttt tcccatcagc tcctcttcgc
     4441 tgtagtacgg caccaggctg gtcacggttt gcagtgtttt ttcccccaca tcaacctgta
     4501 cgatgtacag cttgtcggcg ttttcatggc gtttcacttc cacaatcttt ccgacgcgca
     4561 tttccagacg tgcaaaatca gcgtaagcca cggtttccat tgcttcctcc cttcaagtaa
     4621 aattttacta aactatagca aaattttttc tcaatcctgt aggctaaaaa tggagaatgc
     4681 aggcgtgatc acattcgtaa gccgctgtgt taccgttaca gcgtcaaaga aacgcgcttt
     4741 atttactgaa aacaggtgac ccgataagca cttcctctac aatgggggcg cacatcaggg
     4801 aaagtaaaaa aggtaaacat ggcaacatta aaagacatcg caatcgaagc tggcgtatcc
     4861 ctggcgacag tatccagggt cttaaatgac gatccgacat tgaatgtgaa agaagagacg
     4921 aaacatcgca ttctcgagat cgccgaaaag ctggagtaca agaccagtag cgcccgtaaa
     4981 ctacagacag gcgcagtcaa ccaacaccat attctggcta tctacagcta ccagcaggag
     5041 ctggagatca acgatcctta ctatctggcg atccgccacg gcattgaaac tcagtgcgaa
     5101 aagctggcga tcgaactgac caactgttat gaacacaacg gattaccaga cattaaaaac
     5161 gtcaccggta ttttaattgt cggcaaaccc acgcccgccc tgcgcgccgc tgccagcgcg
     5221 ttgaccgaca atatctgttt tatcgacttt cacgaacccg gcagcggtta cgatgcggtg
     5281 gatatcgatc tggcacgcat cagtaaagaa atcatcgact tctatatcaa ccagggcgtt
     5341 aatcgtattg gttttattgg cggtgaagat gagcctggca aggcggatat tcgtgaggtc
     5401 gcctttgcgg aatatggccg actgaaacaa gtggtacgcg aagaggatat ctggcgcggc
     5461 ggtttttcca gttcgtcggg ttatgaactg gcaaaacaaa tgctggcgcg ggaagactat
     5521 ccgaaggcac tgtttgttgc ttccgattcc attgctatcg gcgtactgcg ggcaattcat
     5581 gaacgtggcc tgaacatccc acaggatatt tcgcttttca gcgttaacga tatccccacc
     5641 gcgcgattta cctttccgcc gctctccacc gtgcgcatcc attccgagat gatgggaagt
     5701 cagggcgtta acctggtgta tgaaaaagcc cgcgatggtc gcgcgctgcc gctgctagtc
     5761 ttcgttccca gcaaattaaa actgcgcggc acgacccgtt aaatcccctt acacactgtc
     5821 cggcaatcat ttttgccgga cag
//
LOCUS       DAZPJP010000233         5779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPJP010000233 DAZPJP010000000
VERSION     DAZPJP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.236"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHU9J_003896"
     CDS             complement(<1..661)
                     /locus_tag="ACHU9J_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1907329.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHU9J_003897"
     CDS             761..1351
                     /locus_tag="ACHU9J_003897"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR1907330.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHU9J_003898"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHU9J_003898"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1907331.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHU9J_003899"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHU9J_003899"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1907332.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHU9J_003900"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHU9J_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1907333.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1506 a         1349 c         1424 g         1500 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcatggtt tttatgcaga tacggagtaa atatttgatt
     5701 atccaaataa aaataaattt taaaaattaa caaatgagtt gaattttttc cgcatcctcc
     5761 gctaaaacag ttaattaaa
//
LOCUS       DAZPJP010000234         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPJP010000234 DAZPJP010000000
VERSION     DAZPJP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.237"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHU9J_003901"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHU9J_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1907334.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHU9J_003902"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHU9J_003902"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1907335.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHU9J_003903"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHU9J_003903"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1907336.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHU9J_003904"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHU9J_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1907337.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPJP010000235         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPJP010000235 DAZPJP010000000
VERSION     DAZPJP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.238"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHU9J_003905"
     CDS             complement(<1..513)
                     /locus_tag="ACHU9J_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1907338.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHU9J_003906"
     CDS             complement(759..1211)
                     /locus_tag="ACHU9J_003906"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1907339.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHU9J_003907"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHU9J_003907"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1907340.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHU9J_003908"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHU9J_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1907341.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHU9J_003909"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHU9J_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHU9J_003910"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHU9J_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1907342.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHU9J_003911"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHU9J_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1907343.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPJP010000236         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPJP010000236 DAZPJP010000000
VERSION     DAZPJP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.239"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHU9J_003912"
     CDS             complement(104..418)
                     /locus_tag="ACHU9J_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1907344.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHU9J_003913"
     CDS             complement(423..638)
                     /locus_tag="ACHU9J_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1907345.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHU9J_003914"
     CDS             complement(789..2642)
                     /locus_tag="ACHU9J_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1907346.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHU9J_003915"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHU9J_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHU9J_003916"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHU9J_003916"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHU9J_003917"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHU9J_003917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHU9J_003918"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHU9J_003918"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHU9J_003919"
     CDS             complement(3908..4966)
                     /locus_tag="ACHU9J_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1907347.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHU9J_003920"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHU9J_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHU9J_003921"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHU9J_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1907348.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1339 a         1583 c         1320 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccactaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPJP010000237         5574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPJP010000237 DAZPJP010000000
VERSION     DAZPJP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.240"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(481..4023)
                     /locus_tag="ACHU9J_003922"
                     /pseudo
     CDS             complement(481..4023)
                     /locus_tag="ACHU9J_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4091..>5574)
                     /gene="tssI"
                     /locus_tag="ACHU9J_003923"
     CDS             complement(4091..>5574)
                     /gene="tssI"
                     /locus_tag="ACHU9J_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1907349.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          986 a         1824 c         1461 g         1303 t
ORIGIN      
        1 acataaaaat atcattaaaa atacacatta caaccccagc gtcccaagct cttcaaaaaa
       61 cttcattgga gaatctttga tattctcaat cttttcacca taaacgtcta atattgatgg
      121 ttcaccaggg tcatcaaact gctcaccata aggtaaaata cccaataaat ttctccaaca
      181 cctatctcta ccattttcct gaagtgattc tgataattgt aaatttgctc tgttatatgc
      241 agctgtcgca gattttatat tccacgcccc taaagccata gaagctgcac cgactggggc
      301 agcaatgatc gttccatcgg cagcaacact tataccgaat tttgtcatgc cacttgcata
      361 cagccttgct gaattagcag tatttaaact agctgtaaac atttttgtcg ggttaaacgg
      421 aaggttatcc agtttattat ttatatcatc tataaaatga ctacaactct cacatgagaa
      481 taaccccaac ggatctatcc tgctcaccgg attcagcgga tactgataca gattccaccc
      541 acctttcagc ccaatggggt cctgggtgat gtaccgtctc tgcaacggat catagtaccg
      601 gtgacgattg tagtacagcc ccgactcctc atcatactgc tgccccggca gacggtatgg
      661 ctgatacaca tgatgcgggt tctcctcatt tagctggttg ccccactcat catacgcccc
      721 gctccacacc gtatcgccgt cttcgctgat gagcgccagc ggctggcccc gatggtcgca
      781 gtggtaaaga tgaacgtttc gcactggtgt gtattccggc tccacctgtc tggccagttg
      841 ctccaccgtc agcccgcact gcgcaagcca cacccggctt tcactgctca cgcggtctgc
      901 ccggatttct tcctccagtc tgtccagcag ccgcaccagt tcagccggaa ataccacgcc
      961 gtgcccgtcc tcgctccctt cctgctgcag cttctccgcc aggctgcggc gctgcgcttt
     1021 ctcccgctcg ccgttctctg tctcgacccg gatgagcggc gtgaagcttc ccggctcgta
     1081 taccgtctgg atacgtgtgg tgtcggtctg taccgtcgtc agcctgtcgc cgtcccagcc
     1141 gtaccatgtc tcctccggtt tacgcgacag cgacatccac cccgtcaggt cacgctcccg
     1201 ccgccatacc cgtttcgcca ttcgccgtcc cagcgggtcg tagaggtagc ggctctcgac
     1261 cagtggctcg ccatgctgta tccgcgtgtg gaacaccagg cggtgctggc tgtcgtagtg
     1321 gtgggtccgc tcgtcgtccg tccgtatcac acccgtcggg atgcggtccg tcttctccgt
     1381 cagtctgccg tattcatcat agtggtaaac atagtgcgca tcctctgcga tgcggttatc
     1441 cggccacgcc gtgagcgtgc tgtccgggtg tagctccggg tccggcaggc ggtttcccgc
     1501 cgggtcggtg gcatacggga tgcggatgtc caggtccggt gcgagggtgc gcaccgtctc
     1561 acggtgcagc ctgtcccgcg tatactcgac cagcggcgtc ccgcccagct tcatgcccgc
     1621 caggtaaccg ctgccgtagg tcagccactc caccggcggc aggctgtccg gcgtgacgcg
     1681 gtttgccagc ccctgttcgt tgtaggcgtg tttcgtctca tgctgccaca gcagttcccc
     1741 cgtctccggg ttctccaccg tctgccgttc gcccgtaagg cggcccttat cgtcgtagcc
     1801 gtagtggaca gcgacacggt ggccttcgct caggtggctg atttctctca gccagccgtg
     1861 gccgtcatac tgccactgct ctgccgggtc gccgttcacc gtccggtgcg tgatgcggtc
     1921 cgacgcatcg tagtgccaga gggtgacaag cccctcatcc tcactctgcg tgagttttcc
     1981 ggtcaggtca taatgataac gttgcgtccg cccgtcaaag ccgccctgct gtaccagccg
     2041 gtccagcgca tcgtagctga agtcgctgtg actgccgttc tcgttagtca gactgatgac
     2101 acgtccggca gcatcgtact ccatactgcg cgtcagcccg ccctgcgtgg tgctgaccgc
     2161 ctttccccac gcatcgtact gtgtctcgct ccggttgccg tccggggtga taacggcagt
     2221 caggtcgcct gcggcgttgt actcatactg cgtttcacgg ccctgtgcgt ctttcaccga
     2281 ggttaaccgg ccacggttgt catagtggcg gtaaaggctg ataccttcct cgcggtgaac
     2341 cgccgtcatc tggccgaagc ggtcgtattc atagcgggtc tggtagcccg agcagtcagt
     2401 gaacgccagc aactgcccgt agcggctcca cgtcatctgc cgggtgctgc ccgtcgcatc
     2461 cgttgtcgtc gccggtaact cactgtgcgc gtcatcgtag cggtagcgta ctgtctcccc
     2521 gctgcgcgat gtctccgata ccagcctgcc cggttcatca tattcccggc ggctctccag
     2581 cccgtccggg tacaccaccg ccgtcagctg gttcccgtcg ttatagtaaa atttcgtctc
     2641 ccgcccgtcc ggtgtggtga tgtccgtgat atcgccggac accacattca gaccgtactc
     2701 tgtccgccgt cccgccgcgt ccgtctgcgc cgtgagcctt cctgccgcgt catacccgct
     2761 gcgcgtgacg ctgccgtccg ccagttcttt tttcaccacc cgtttcagcc cggccccgcc
     2821 ttctgtatgc agcacctcac gccggttcag gctgtcggtg acggtgatgc ggtcctgctc
     2881 ataaagatag cggtagctta accctgccgg gttcagttgc tccaccaccc gccccgtatc
     2941 gtcgtagcgg tagcgcatct ccggccttcc cgcgtaacgg tgcgccacca tccggcccgg
     3001 gtgctgcgcg tcatacgtga aagcgcgcac ctgcgtattg ctgcggtcat ataccgccag
     3061 cagttcaccg gcttccgtat acgtgtaccg caccagtggc gcagcgggca ggctctccgg
     3121 gtatgccggg tcgtgcatca accacaccgc cgaaaggcgg atacccctgt cggggccgta
     3181 ttcggtacca ggcagtgtgt cggggaacgc tgaggctgag agagggcggg aactgtcaga
     3241 agaagatagc gaagaggtgc gggcctcttc cgcacgctgc gcctgcgtgg tcagcaccag
     3301 acggaactcc cgcccggcac cgtccgtcac gtcggtgatt tccccggcca ggtcaccggc
     3361 ggcctcacgc cggtacgtca gcgtccgcca tcccggtaag cacccggtac ggcggcagcg
     3421 gcgctggcag tacgtcctca gcacccggca cccgctcagc cccccccagt atccaccacg
     3481 gcccctgtgc gctgttggtc gccaggtaaa gatgcgggct taaccggata tccggcgcag
     3541 cgccccccac agccgcgcca gcgtatggcc gtccggctgt gctgccttac caccgcgcac
     3601 cagccacatt gactcactgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
     3661 aatgctccgc ccgccgttgt cgttgagtat cagtccgtca tcacgtagct gtaagcggat
     3721 atcagaaggc gctttccagc cggggccgaa aacgcccacc ggtgcaggcg tcttcgtccg
     3781 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
     3841 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
     3901 gcccggacac accgagcacg ccacgccggt gggcgcgcca attcttacac ctgccgaacc
     3961 ctggacaatg ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
     4021 catgattcaa attccttctt ctgtttattt ttaatgctgg tttgttactg aataccgaac
     4081 aggtacggga tcagtactct ctcatatccg gcgtatcctg cttttcatca ggggtcttcg
     4141 gtggggattt cgaggctgca cagttccagt taatcaacag tgagtcacca ttaacctgct
     4201 ccttaccctg caaatgaatt tttgtgccgt tgagaaaaat ttgtccgtcc ttcgtcagca
     4261 ccagccttgc cttaccacag cagagctcca gatgctcacc ggcggagtaa atcgcggtct
     4321 gcccggtatc atcctttttc gttttaccaa cggtgaaggt aacgttattt ccgactttga
     4381 cgtcataacc cagcccgacc ctcaacgatt tatgcaaacc gatctgcgag gactgcatca
     4441 gtgccaccga ggtgttcata atgccaccta ccgtcgtctg atacgccacg cccaccgtca
     4501 gcgcacgaac aagtccaatc gtttccacct gaacagaacc cactttaatt atctggttac
     4561 tgcccaccgt ctctatctgg ttaacgccca ccgtctctat ctgattgttg gtaaccgcaa
     4621 tcatctgatt gttgccaatg gtttcagcat ggttgtttat cacatccgtg gtgcggttat
     4681 tcagcacctc ggtgttcatg ttcttctgcg cgtggatgta gacctgttct ttccctgtcg
     4741 catcgtcaaa cttcagttca ttaaacccgc tgcccttata ggttttcgaa cgaatggtca
     4801 tctgcgtctt cgtccccggc aggctgccgg gtgtgcggtt ttcctggtgg taggtacgcc
     4861 ccataataat cggctggtcc gggtcgccgt tgaggaagtc cacaatcacc tcctgaccca
     4921 cacgcggtat cgccaggttg ccaaagccgg tgcctgccca cgcctgtgcc acacggatcc
     4981 agcatgaact gtcctggttt gacgggttat agcggtccca gttaaatttc acccgcacgc
     5041 ggccatgttc atcacagaag atttcctcgc ctgccggtcc cgtcacgacg gcgctctgcg
     5101 ggccatccac cagcggtttc atcaacggct gtggccgcca tgttctgtct gccgggatta
     5161 ccgcaaaatg gttctccagc gtggtgcctg aacccctgcg tcccggtacc gcctgcggct
     5221 gttcgccgtg aagatcgctc gccaccacct gccattcccg gttcaggttc gcctgcggat
     5281 gccccgtcag cacaattcgc cgtcccggcc atatctccgg cgaacggctt gttccgcgcg
     5341 ccacttctgc gttgtttcgc cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg
     5401 cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg taatcctggt
     5461 actggccttc ctgatcaaaa cgccctggcc agccgggacg tttaaaggtg tagtctttgg
     5521 tcactacgga agaaggacgg atttgtgcgc tgtagcgaaa ctggctgatg caga
//
LOCUS       DAZPJP010000238         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPJP010000238 DAZPJP010000000
VERSION     DAZPJP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.241"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHU9J_003924"
     CDS             complement(<1..1369)
                     /locus_tag="ACHU9J_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1907350.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHU9J_003925"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHU9J_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1907351.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHU9J_003926"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHU9J_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1907352.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHU9J_003927"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHU9J_003927"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1907353.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHU9J_003928"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHU9J_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1907354.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPJP010000239         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPJP010000239 DAZPJP010000000
VERSION     DAZPJP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.242"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHU9J_003929"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHU9J_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1907355.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHU9J_003930"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHU9J_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1907356.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHU9J_003931"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHU9J_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1907357.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHU9J_003932"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHU9J_003932"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1907358.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHU9J_003933"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHU9J_003933"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1907359.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHU9J_003934"
     CDS             4447..4812
                     /locus_tag="ACHU9J_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1907360.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHU9J_003935"
     CDS             4962..5480
                     /locus_tag="ACHU9J_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1907361.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPJP010000240         5523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPJP010000240 DAZPJP010000000
VERSION     DAZPJP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.243"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(333..1121)
                     /gene="phoH"
                     /locus_tag="ACHU9J_003936"
     CDS             complement(333..1121)
                     /gene="phoH"
                     /locus_tag="ACHU9J_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1907362.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1742..3013)
                     /gene="efeB"
                     /locus_tag="ACHU9J_003937"
     CDS             complement(1742..3013)
                     /gene="efeB"
                     /locus_tag="ACHU9J_003937"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1907363.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3019..4146)
                     /gene="efeO"
                     /locus_tag="ACHU9J_003938"
     CDS             complement(3019..4146)
                     /gene="efeO"
                     /locus_tag="ACHU9J_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1907364.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4204..5034)
                     /gene="efeU"
                     /locus_tag="ACHU9J_003939"
     CDS             complement(4204..5034)
                     /gene="efeU"
                     /locus_tag="ACHU9J_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1907365.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1378 a         1515 c         1309 g         1321 t
ORIGIN      
        1 tattcggtgt taaacagtca gtcaaagtat agcccattgt tgattaaacc tcataagcaa
       61 aattggcacc agatctcctg tacaggttga atggccgaaa aaagataggc agatgctcat
      121 ttttttggct tctggaagaa aaactctccg cgctggcagt attacactgg tctgatgcta
      181 tgacgaaaaa tgcctggtaa gagtatctaa accagactcc agttcagctt atcaacacgc
      241 tcaaagtcgc ccccctgaac gctaacctga agtgaaaatc tgttgaaata aaacaaagcc
      301 cggttcgccc gggctctgca ccgataacac acttaactgt aggcatgcag cgtacgttgg
      361 caaagtgccg aacgtacgca gtcctcttta ccgaaccgga cgatcccaac catttcatct
      421 tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg caaatcgcat
      481 tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt taaaaacatt
      541 ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc attttcaaag
      601 gtacgtccac gcatataggc gaacggcgca atttccacct tccctatttc cggtcgcagg
      661 cagtactgca taaaggaagc ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa
      721 tagggagcaa acttttctgc gatatctcca ggtaagaagc caagatcttc atcggcttgc
      781 agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc ctctgccgct
      841 tttgctgcgc tgatccaggt ttttccgccc ccggcttcgc ccgtggcgaa tatcagctgc
      901 ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag gatgggcgaa
      961 gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgcccatctg cacaagcgag
     1021 gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt
     1081 gcctcgcgac gagctttgat cactgctttt tgtcttccca tagagagcac cttgagttgt
     1141 ttgtattcat cacacgcgcc gttggcagcg cgattatgcg cacgaacatc agagggttgg
     1201 cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct acgcgcaccg
     1261 tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt gaaaatgcaa agtgacgaga
     1321 tgactaccgg aggagaaaac tccgcgagtg gtggcgcgtt gattatctaa aacatgtcca
     1381 gtacaggacg ttaccatccg cgatctccat agtgactgac tatcactgcc gggaacttcc
     1441 gctgctactt aataagtaca acagatctcg catttattgc aacaatatat ttacttatat
     1501 ttaactataa aacactattt cagtgacatt agtttctact ggaaagatga cagagtgatg
     1561 acagtgatga aaaaagctgt gtgctttcag caggatttgc atcaggcatc agtgcacgat
     1621 cgccggatgc ggcgtgaacg ccttatccag cctaccaaac ccgcactgat tgtaggcatg
     1681 ataagacgcg caagcgtcgc atcaggcatc tgcgcacaaa tgccttatcc gcctaaaaac
     1741 attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg
     1801 aaaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc
     1861 ttttttgtac tgtcaggaag cctttttcca gatcgtgttg gtagcagaca aacagcaacc
     1921 ccatatccag ttgtccggag ttggtgaccc ccaacgaata actgtagccg cgacgcagca
     1981 tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg
     2041 caatcacctt cccttccggg tcgctggcgt aatcaggcac atcatgctca tgctgcattc
     2101 ccagcggcgc accggtttgc ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg
     2161 ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga
     2221 ttgtccacgc aggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat
     2281 cattctggct atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct
     2341 ctttgccttt actacgcgac acgtgatcgg aaataaaccc ttcccgcttc cagcgcacac
     2401 tgagcaaatc cggcgtgtgt ttgatgatat cgcgcagcgc atggataacc gtgtcctggg
     2461 tgttggcgca aatctgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt
     2521 tggggaaacg cgtcatcttc tgcagctttt ttggcatctg tggcgcaagg ccaaagcgct
     2581 catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc
     2641 cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgctgctc
     2701 cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgc tcaagatcgg
     2761 ctttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca
     2821 ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa
     2881 gcgtgcccgg tgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg
     2941 ccagcgcacc aatccctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat
     3001 ctttatactg cataacgctt aatccagtcc cagcacaccg cgaagttgcg ccagatcttc
     3061 cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta
     3121 ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt
     3181 ggcatcgact tttgccagca gttccgggtt ggctttttgc agttgtggac gcagcaaatc
     3241 gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta
     3301 gcgatcttct tcaccgctaa ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc
     3361 gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac
     3421 atctgtataa agctgctcag cgtactgatc catccctttg gtggtgttgt cgccaaacaa
     3481 tgctttttcc agacggtgga aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc
     3541 atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat
     3601 gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc
     3661 tttaatcgcg tcggtaaagg ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac
     3721 atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc
     3781 atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca
     3841 ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc
     3901 gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt
     3961 ctggctatgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg
     4021 ttcgcactgc ttatccgtca cggtcacttt gacctgcggc acatcagcgg cgttagccat
     4081 aaaagcagaa gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat
     4141 ggtcatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat
     4201 ttgttacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag
     4261 cgccgggatg agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata
     4321 gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagca ctgcgctcat
     4381 atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc
     4441 accagcagcc agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc
     4501 accaagattg aggcgaatac cgccccagta gagcaggaag cctagcacca cggcagtagc
     4561 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
     4621 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
     4681 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgct ccagttgtac
     4741 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
     4801 gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt cgccggtggt
     4861 ttcgttaatg aagataccca agcccaggca caacgcagcg gcaagcaaca cgccaatcca
     4921 catcacgcca atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac
     4981 aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc
     5041 cacccttaaa cgtttttctg agtcaatcca tgcaaagaaa agtaaatcgc agtgatagcg
     5101 attatcatta cggcaaagaa aaatacaagc ggaatgttta taaaatgatg ttggaatgta
     5161 aacaaagaga ggtaatacca ggggatataa acgattattc caggtacaaa gatgggattg
     5221 tttacaacga gccatgtgtt cttgccagat gccgcgtaaa cgccttatcc agccgacaaa
     5281 actgtccaga ctcaatgcat cacagcttcc atgtaaaact gataagcggg tgtatcaagc
     5341 aatttcgtcc atccgcgatt atcaaatcta actttttgcc cggaacataa taacagcgcg
     5401 ggtgttggtg gcgtcaaaat cgctgaaacg ttccccgaag gccggggcag cccacatgga
     5461 tgtgggctga gggcgcgtct tacaggacgt tacctcgcgc ccgacccggt agccgaaggg
     5521 gat
//
LOCUS       DAZPJP010000241         5485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPJP010000241 DAZPJP010000000
VERSION     DAZPJP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.244"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..115)
                     /locus_tag="ACHU9J_003940"
     CDS             complement(<1..115)
                     /locus_tag="ACHU9J_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1907366.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHU9J_003941"
     CDS             complement(220..1722)
                     /gene="dtpA"
                     /locus_tag="ACHU9J_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1907367.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHU9J_003942"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="ACHU9J_003942"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1907368.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHU9J_003943"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="ACHU9J_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1907369.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHU9J_003944"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="ACHU9J_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1907370.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHU9J_003945"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="ACHU9J_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1907371.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5351..>5485)
                     /locus_tag="ACHU9J_003946"
     CDS             complement(5351..>5485)
                     /locus_tag="ACHU9J_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFR1907372.1"
                     /translation="RKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
BASE COUNT         1444 a         1390 c         1350 g         1301 t
ORIGIN      
        1 tttcgagacg ttttttcatt aaatccacac tgacgagggt aaaatccttt ccgctctcac
       61 gcagggtgat atgggaagcg agagagcagg cacccggttt gtagaacaat ttcatcggta
      121 actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaagccgcta
      181 acgaaaagtt agcggcttgt aaaagagttt ccctgaattt tacgctacag cagctttcgc
      241 cgctttgtct gcagcgtcat cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat
      301 cagtactgca ataatggcag tagcgacacc aatctgcaag aatacgcgac cataaacttc
      361 cagtgacatc agcgggtcgg taacgttatc cggcacagcc atcatacccg caacataacc
      421 accaatcagg tttgcaccgg cagtggtcag gaaccagcta cccataatga agcccatcag
      481 acgctgcgga acgagttgtg caaccattgc cagacccaga ccagagatca tcagttcccc
      541 gatgctctgc aggccatagc ttgcgaccag ccagcttaca gacacgatac cagcgtcaga
      601 cgcgaatttc gcacccagcg gcagaatcag gaacgcacca gaacacatca ccatgccgat
      661 tgcaaacttg gttggcatcg gcagggtatc gcccatcttg ttatagatag cggccagaat
      721 cggactaccg atgatgatcc agaacgggtt cagtgcctga tactgctcag gttctacggc
      781 cagacccaga atggagtgct caacgttacg aatcgcaaag aagttcagtg acgttggcat
      841 ctggctgtac agcacgaaga agataatggc ttcgagcatc aggatgaagg caacgatcat
      901 tttacgacgc gcagcacctt tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc
      961 gaaggcaaca acgcccagcg ccatacgcgc aacttcctga ttgtgcagca gccaggtggc
     1021 gatagcgatc agtgccacaa caccaataat ggtcagcagc aggttacggt agttgatagg
     1081 ctcgaagtct ggttttgaac cgtactgttt aacccagcgt tggcagaagg cgaagttaac
     1141 gatagtgatc agcaggccta caacgctcaa cgcaaacgca acactccagc cgtatttcgc
     1201 ggccagccac ggcgtagcaa tcatagagaa gaaagagccg atgttgacgg acatgtagta
     1261 catggtgaat gcaccgtcca gacgcgggtc gtttttctca tagcatgtag aaagcagaga
     1321 agatgggtta gctttaaaca ggccgttacc gaccgcaata gccgccatac ccatataaac
     1381 gataccggcg tcgtgaccag accaggcaac cagagcataa ccaatcgcca gcacaatagc
     1441 gccgagcata attacgcgtt tagtacccag taccttgtca cctaaccagc cgccgatagc
     1501 gaccagacca taaaccaggg cactaaagga agagaaaagg gtgattgaat ccgcttcaga
     1561 catacccagt tgtttaacca ggtaaacagc cataattcct tgtaggccgt aataaccaaa
     1621 acgttcccat aactcaatcg agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt
     1681 caaactgacg ctttcagttg gtttttggtt tgcagtggac acttttacct ctttttttac
     1741 atcccatatt aacggggggg ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt
     1801 atagtgggag gggaaacggc aggtaatgtt cactatcttg cactttcaga caagtccttt
     1861 gtaataatct gttacatata actgaggcgg ggaattcaac cattccggga aatgttatcc
     1921 agcgttaaat ttttcaaaaa acgtaatggc agattttttc actgcactga gagggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tggcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tagtctgttg ctgttccagc ttgcg
//
LOCUS       DAZPJP010000242         5474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPJP010000242 DAZPJP010000000
VERSION     DAZPJP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.245"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..169)
                     /locus_tag="ACHU9J_003947"
     CDS             complement(<1..169)
                     /locus_tag="ACHU9J_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907373.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQ"
     gene            complement(313..1071)
                     /gene="aroD"
                     /locus_tag="ACHU9J_003948"
     CDS             complement(313..1071)
                     /gene="aroD"
                     /locus_tag="ACHU9J_003948"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1907374.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1102..1968)
                     /gene="ydiB"
                     /locus_tag="ACHU9J_003949"
     CDS             complement(1102..1968)
                     /gene="ydiB"
                     /locus_tag="ACHU9J_003949"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1907375.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1980..3245)
                     /locus_tag="ACHU9J_003950"
     CDS             complement(1980..3245)
                     /locus_tag="ACHU9J_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907376.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3472..4686)
                     /locus_tag="ACHU9J_003951"
     CDS             complement(3472..4686)
                     /locus_tag="ACHU9J_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907377.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMGLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4786..5142)
                     /locus_tag="ACHU9J_003952"
     CDS             complement(4786..5142)
                     /locus_tag="ACHU9J_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1907378.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5278..5385)
                     /gene="rprA"
                     /locus_tag="ACHU9J_003953"
     ncRNA           complement(5278..5385)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHU9J_003953"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1594 a         1250 c         1237 g         1393 t
ORIGIN      
        1 tctgtttata tttatcagca agagctgtaa ttaacgtggt ggcttccaga ataccgccgc
       61 cagcgcctaa cacacaaagt gttgcttcgt cgggaatata attcaccgct tcctgtgccg
      121 acaggaccgg cactcgtcca ttaatacgag gtggttttac aggtttcatt gttaattcct
      181 gattcagatg tgattttccc tacgccagat ggagaattag cgcacagaga ctcacgaaat
      241 atttttatta aatacggtta aatatatttt ttaagttcgg cggggagggt gttcccgccg
      301 aaatattatt gcttatgcct ggtgtaaaat agttaatacc gtgcgtaaat catttaccga
      361 gatttgccct ggtgcagagg ctttttttac cgcaccaaaa gttgccgccg agccaaatac
      421 ttcaccagcc agacgagaaa ttacgccagt ttttgccatc gacatcgtga tgattggacg
      481 atcggcatac tgctcctgca tctccagggt cgcggtaagc aacgtcagca catcgctggt
      541 actttgcggc atcagcgcaa tcttaggaat atcggcgtcg aaggactgca ttttgcgcag
      601 acgggcaatg atttcttcgg cttccggcgt tttatggaag tcatggttgg acatgacaac
      661 tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta acctgatcat cgccggtaaa
      721 taactccaga tcgatcatat caaccaggcc gctgtcgatg gctgcacgat tgagagcaat
      781 ataagcctcg gtggaaatcg cctgctcgcc gccttctttg gcactgcgga aggtaaacag
      841 cagcggtttt tctggcatgg tttcacggag aatttttgcc gccgccatga cagactccac
      901 attggagagg tcggcaaagt ggtccacacg ccattccaga atatcaaagt ccgcttcacg
      961 ataggcgaga gcttcggatt tcacgcgggc gatatctttc gccatcagcg agacgatgat
     1021 tttaggtgcg cccgcaccaa tgacgagatc ttttacagtt acggttttca ttttttaccc
     1081 tttctgcacg cggtcagcct gtcaggcacc gaaccccatg acctgtttaa catattccag
     1141 agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc ccttgccaca acaacatgcc
     1201 gtatccatca atcgttttgc aacctgcttg ttgtgcctgc tgcaataact tcgtcatatg
     1261 cgggttatac acgcattcag tgaccagcag tcccggatgt aacagactga tatcattaac
     1321 caatgattca ttctcaaggg ggttcatacc tacttttgtg ccattggtta aaatgtcggc
     1381 ggaagccagg acttcagcaa aggcttgctg atcggcgaga tcggtaaccg tgacgacaca
     1441 atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct ttatcgaaga actcatcccg
     1501 acggttaaag agtttaattt cttttaaacc ttcaattgcc ccctgcgcgc caatcgctgt
     1561 tgaggcacct ccggccccta acagcaccat cgttttgcct ttgatatcaa aaccgctctc
     1621 tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag ccacgcagat agccatcatc
     1681 attaacgatg gtgttgatgg cacccaccag tttggctgct ggcgttaatt catcaacata
     1741 ttcacacgcc agttgtttgt tcggcatcga cacaccagtt ccgcgcattt tgagggcttt
     1801 taatccttca attgctccgg gaaagctatc gttatccact tcgaaggcca tataggtaaa
     1861 tggcaatccc gctttttcta aggctttatt ctgcatttca ggcgataaac tgtggcggat
     1921 aggataagcc atcaacccaa tcaattcgta ttttgcggta acatccataa ttaactcctt
     1981 taacctctat gcttaattgt gtttaaccgg gtgctaaatt tgcgttcgcc aaaccgcaca
     2041 tcattctcag gaataataaa aacgcggtaa tagcggctaa aaacaataat tgcggtaatc
     2101 agggccagca gcgcgaaagt aaaatcgaga acaatgatat attgcaggcc gatgttcgac
     2161 agataaccgg taatcagcgg aataacaaag ttagccagtc cacccatcat catataaata
     2221 ctggtgactt tggctttgct tttggggaaa aactctgaca tgaccgaaac accgagctgt
     2281 aaaatgccgc cagctgctga gaaaccgata acaaaggcgc cggcattaca caccagtgga
     2341 gaagggtaca ggtagataat ggcagctgtt attgttgcca gcgcagagtt aaatacatta
     2401 gcccagatgg gccggaccat ttttttcagt agtgcggcaa aaataaagac acagaccaac
     2461 gagcccatac tgtaataaga gatggttttt aatgcctcag cttctgacat accagcaaaa
     2521 gccatcgcat atttgggcat ccacaccaca atcacataaa aggtcgagaa tgcggctaca
     2581 ccgaacagta ccgatgaaac accttccagc cagactaacg gtttgctgtt catttgcggt
     2641 aattcattag ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc
     2701 agcgtgatca gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta
     2761 ttgagcaaca tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct
     2821 ttaaccagta tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag
     2881 ccacccgtat ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga
     2941 gttaaattcg ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt
     3001 aatatcaccg cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc
     3061 aatcgcccca aaccgatacc ggaaattaaa taggcaatgc ccgcgttgtc agtggaaaac
     3121 ttttctgcca gagatgtcat attttgggca agcgtaataa cactaatgcc gtgcaggaaa
     3181 tagctgaagt aaatacaaag aacagccagg ataaatggcg tgctgaaagc cttattttga
     3241 gacattttca cagctccttg ctgaaagata atttcgttac cgtacgtatt gttctccgac
     3301 ggtttacata attactatac tgaagatgat tttccctggg tacatggcat aaagtttatg
     3361 tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt agttagcgca
     3421 gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc attacccacc
     3481 cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat
     3541 aaacagtgcc agtaaaaaac cgatggcagc gatggcggta tcgaaccaca taatatcggc
     3601 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
     3661 cgcactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
     3721 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
     3781 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
     3841 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
     3901 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttgatcg acatagtgta
     3961 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
     4021 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
     4081 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
     4141 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
     4201 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
     4261 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
     4321 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
     4381 gccaaaaaca taagcgataa tgatgttatt ggtctgcagg atgccaaaaa agaaggccat
     4441 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
     4501 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
     4561 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggccca tcaaaatgac
     4621 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
     4681 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
     4741 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
     4801 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
     4861 gccatcaggg tagacttgtt gaaacgcggt taagtcgggg aaaaaacgca tggtgttgtt
     4921 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc gttgctgacg
     4981 catagccaac agttgctcga ctacacaatc aggaatggcg catttgccat tttcccactg
     5041 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
     5101 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
     5161 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
     5221 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagcgg aaaaatgtta
     5281 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
     5341 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
     5401 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
     5461 cgacaggtac tacc
//
LOCUS       DAZPJP010000243         5471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPJP010000243 DAZPJP010000000
VERSION     DAZPJP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.246"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(223..318)
                     /locus_tag="ACHU9J_003954"
                     /pseudo
     CDS             complement(223..318)
                     /locus_tag="ACHU9J_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(315..881)
                     /locus_tag="ACHU9J_003955"
     CDS             complement(315..881)
                     /locus_tag="ACHU9J_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1907379.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1191..2963
                     /gene="aspS"
                     /locus_tag="ACHU9J_003956"
     CDS             1191..2963
                     /gene="aspS"
                     /locus_tag="ACHU9J_003956"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1907380.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            3081..3533
                     /gene="nudB"
                     /locus_tag="ACHU9J_003957"
     CDS             3081..3533
                     /gene="nudB"
                     /locus_tag="ACHU9J_003957"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1907381.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3562..4302
                     /locus_tag="ACHU9J_003958"
     CDS             3562..4302
                     /locus_tag="ACHU9J_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1907382.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4337..4858
                     /gene="ruvC"
                     /locus_tag="ACHU9J_003959"
     CDS             4337..4858
                     /gene="ruvC"
                     /locus_tag="ACHU9J_003959"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1907383.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4860..5462)
                     /locus_tag="ACHU9J_003960"
     CDS             complement(4860..5462)
                     /locus_tag="ACHU9J_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1907384.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1375 a         1320 c         1486 g         1290 t
ORIGIN      
        1 taactgtatg gataaacagt gtcaagagtg agggaaagtg attttttggg gcatgcatgg
       61 ggcaaaaata tttgttgtgg ggcattttgg ggcatgattg ggacactttg ttacatatga
      121 actttgccga ttttcatatg gcatggcaat ccaacgcttt gattattcta aagaaaacac
      181 atgctcttgg gcgttcttta gtgattttta aaaatcccgt atcacgcagt taaagtggcg
      241 ggcatactct tcaaggctgg tgatccccaa ccgcacccat ttaggatgcg accattgcgg
      301 tagaccgatg taaatcataa cgcgtgaagg atctcttcca cgctacgcac acgggcgatg
      361 cgcgggtaga tatgattaat gctgttattg tgttgctcgg cgctggcggc actacaggcg
      421 tcttcggcaa tcaccagatt aaagccaagt tcccaggcat tgcgggcggt ggattcaaca
      481 ccgatattgg tcgagatccc acataacact attgtatcga tacctcggcg gcgcagttgc
      541 aactccagat ccgtaccgta aaacgcaccc cactgacgtt tgatgatttc gatatcactg
      601 tcagttgcac ctaatgcagc tggatgttgc caccagtttt cgggcaacac tttagcgggg
      661 gagggggcat caaccggctg ttttaatgct tcggcgtaat cggcagacca gccaactcgc
      721 accagaaaca cgggctgacc gctggcgcga aatttcgccg ccagcttccc ggcgcgatta
      781 actacctcat cggcagtatg tggaccaccg gcaaaaggta agatgccttc ttgtaaatcg
      841 atcaccacca acgcagtggt tttagcatta agttcaagca tgctgactcc tgaaaaaaca
      901 aaatcgtgcc tcacacctta atgcgattag ccgatggcgg aacgacaaat tttgttaatt
      961 tttatgagtt cgggcaacac ctttatagca aactggcgta gagtctgatt tcaggcccga
     1021 tataaagtgg taacgaggcg ttacagcgcg ggccaggctc ttacggtgcg gcagaatttc
     1081 cagtataata gccgcctttt ttcattcagt tgtgacatac agctaacgct gcgacttggt
     1141 cacctgcgca gcagggggca tccgccggca gctaagttaa gggatatctc atgcgtacag
     1201 aatattgtgg acagctccgt ttgtcccacg tggggcagca ggtgactctg tgtggttggg
     1261 tcaaccgtcg tcgtgatctt ggtagcctga tcttcatcga tatgcgcgac cgcgaaggta
     1321 tcgtgcaggt atttttcgat ccggatcgtg cggacgcgtt aaagctggcc tctgaactgc
     1381 gtaatgagtt ctgcattcag gttacgggca ccgtacgtgc gcgtgacgaa aaaaatatta
     1441 accgcgatat ggcgaccggc gaaattgaag tgctggcgtc ctcgctgact atcatcaacc
     1501 gcgcagatgt tctgccgctt gactctaacc acgtcaacac cgaagaagcg cgtctgaaat
     1561 accgctacct cgacctgcgt cgcccggaaa tggcgcagcg cctgaaaacc cgcgctaaaa
     1621 tcaccagcct ggtgcgccgt tttatggatg accacggctt cctcgacatc gaaactccga
     1681 tgctgaccaa agccacgccg gaaggcgcgc gtgactactt ggtgccttct cgtgtgcaca
     1741 aaggtaaatt ctacgcactg ccgcaatccc cacagttgtt caaacagctg ctgatgatgt
     1801 ccggttttga ccgttactat cagatcgtta aatgcttccg tgacgaagac ctgcgcgctg
     1861 atcgtcagcc tgaatttact cagatcgatg tggaaacttc tttcatgacc gcgccgcaag
     1921 tgcgtgaagt gatggaagcg ctggtgcgtc atttgtggct ggaagtgaag ggcgtggatc
     1981 tgggcgattt cccggtaatg acctttgcgg aagcagaacg ccgttatggt tctgataaac
     2041 cggatctgcg taacccgatg gaactgaccg acgttgccga tctgctgaaa tctgttgagt
     2101 ttgcagtatt tgcggggccg gcgaacgatc cgaaaggccg tgttgccgct ctacgtgttc
     2161 cgggtggcgc atcgctgacc cgtaagcaga tcgacgaata cggtaacttc gttaaaatct
     2221 acggcgcgaa aggtctggct tacatcaaag ttaacgaacg cgcgaaaggt ctggaaggta
     2281 tcaacagccc ggtagcgaag ttccttaatg cagaaatcat cgaagccatc ctggatcgta
     2341 ctgccgcgca agatggcgat atgattttct tcggtgccga caacaagaaa attgttgccg
     2401 acgcgatggg cgcactgcgc ctgaaagtgg gtaaagacct tggtctgacc gacgaaagca
     2461 aatgggcacc gctgtgggtt atcgacttcc cgatgtttga agacgacggt gaaggcggcc
     2521 tgacggcaat gcaccatccg ttcacctcac cgaaagacat gactgctgca gaactgaaag
     2581 ctgcaccgga aaatgcggtg gcgaacgctt acgatatggt catcaatggt tacgaagtgg
     2641 gcggtggttc agtacgtatc cataatggtg atatgcagca gacggtgttt ggtattctgg
     2701 gtatcaacga agaggaacag cgcgagaaat tcggcttcct gctcgacgcc ctgaaatacg
     2761 gtactccgcc gcacgcaggt ctggcattcg gtcttgaccg tctgaccatg ctgctgactg
     2821 gcaccgacaa tatccgtgac gttatcgcct tcccgaaaac cacggcggca gcgtgtctga
     2881 tgactgaagc accgagcttt gctaacccga ctgtactggc tgagctgagc attcaggttg
     2941 tgaagaaggc tgagaataac tgatatgact caaatacacg aaatcattcg cgttgcatca
     3001 aggcggcaac tgagtgaact cccatgagca tagataacta tgtgaatggg atgagcgaag
     3061 gcagtcaacg aagaggcagc gtgaaggata aagtgtataa gcgtcccgtt tcgatcttag
     3121 tggtcatcta tgcacaagat acgaaacggg tgctgatgtt gcagcggcgt gacgatcccg
     3181 atttctggca gtcggtaacc ggcagcgtgg aagagggtga aaccgcgccg caagctgcca
     3241 tgcgcgaagt aaaggaagag gtcaccattg atgttgtcgc tgaacaactg accttaattg
     3301 actgtcagcg cacggtagag tttgaaattt tttcacattt acgtcatcgc tatgcgccgg
     3361 gcgtgacgcg taatacggaa tcatggttct gtcttgcgct cccgcacgaa cgccaggtag
     3421 tcttcactga acatctggct tacaagtggc ttgatgcgcc tgctgccgcg gcgctcacta
     3481 agtcctggag caaccggcag gcgattgaac agtttgtaat taacgctgcc tgaacaggca
     3541 gggctatctg gagatatttt tatggcaggt catagtaaat gggccaacac cagacatcgt
     3601 aaagctgcgc aggatgctaa gcgcggtaaa atcttcacta aaatcattcg tgagctggta
     3661 accgcggcta agctgggcgg tggcgatccg gacgctaacc cgcgtctgcg tgcggcggtg
     3721 gataaagcac tgtctaacaa catgacccgt gacacactga accgcgcaat tgcacgtggt
     3781 gtgggcggtg atgatgatgc aaacatggaa accatcatct acgaaggtta cggtcctggc
     3841 ggcacggcaa tcatgattga atgtctgtct gacaaccgca accgtaccgt tgctgaagtg
     3901 cgtcatgcat ttagcaaatg tggcggtaac ctcggtactg atggttccgt tgcctatctg
     3961 ttcagcaaaa aaggcgtgat ctccttcgag aaaggcgatg aagacaccat tatggaagca
     4021 gcactggaag caggtgctga agacgttgtg acctatgatg acggcgcgat tgacgtctac
     4081 actgcatggg aagaaatggg taaagtgcgc gacgctctgg aagcggcagg tctgaaagca
     4141 gacagcgcgg aagtttccat gatcccgtcc accaaagctg atatggatgc agaaaccgca
     4201 ccgaaactga tgcgtctgat cgatatgctg gaagattgcg acgacgtgca ggaagtctac
     4261 cataacggtg aaatctctga tgaggttgca gcaactctct gatggggcct gctaaacagc
     4321 aaaacggaga cgcgtgatgg ctattattct cggcattgac ccgggttcgc gcgtgatcgg
     4381 ctatggtgtc atccgccagg taggcaggca actgtcctac ctgggtagcg gatgcatacg
     4441 caccaaagtg gatgatttac cgtctcgtct gaagctcatc tatgcgggcg tgacggaaat
     4501 catcacccag ttccagccag attatttcgc cattgaacaa gtctttatgg ccaaaaatgc
     4561 cgactcggct ctgaaactgg ggcaggcgcg cggcgtggcg attgtggcgg cggtgaatca
     4621 ggaattgccg gtatttgaat acgcggcacg tcaggtaaag caaacggtgg taggtattgg
     4681 tagtgccgaa aaaagccagg tgcagcatat ggtccgcacc ttgctgaaac tgcccgctaa
     4741 tccacaggcg gatgccgccg atgcgttagc tatcgcgatt acccattgtc acgtcagcca
     4801 gaatgcaatg cagatgagcg aatcgcggct gaacctggcg agagggcgac tgcgttaagt
     4861 tataccgcct cggtcagttc aggctgaggc gtttcaactc cctccgcgtg aatcaacacc
     4921 agacccggat gatgccacaa actggctggc gtgacggttt tacgctccca cggaatagaa
     4981 cctaagaacc agaatttcca gaaagtgagt tttgcattcg gattgctatt taacagttct
     5041 ccaaacgttt ctatttcacc caccggaatg catagcgctt ctttataaat atcaaaaaca
     5101 aattttgaac agaactggcg cgaagattcg tatttaaaac cggtgtggta aagtttgcgt
     5161 agccgggagg gaacctgttc aacaattcgt tgtttttgtt gttctgtcag cccagcgtct
     5221 aatcgcttta tagcatagcg ttgattagac gaacgtttaa taaaacgaga tagcgtggtg
     5281 atggttgaaa ggggaacccg gctttctgca accaggaagt cttcaccgtt atgaccgata
     5341 atgatcccga cgtgattact ccagcaattt gatgcagctg atatttgacc aaataaggca
     5401 gcacctatac atgtaaagac gatatctcca atttcatatt cagcagggta attaatattc
     5461 acgttgtcat t
//
LOCUS       DAZPJP010000244         5407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPJP010000244 DAZPJP010000000
VERSION     DAZPJP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.247"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHU9J_003961"
     CDS             complement(<1..1334)
                     /locus_tag="ACHU9J_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1907385.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHU9J_003962"
     CDS             complement(1380..2138)
                     /locus_tag="ACHU9J_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907386.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHU9J_003963"
     CDS             complement(2170..2883)
                     /locus_tag="ACHU9J_003963"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1907387.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHU9J_003964"
     CDS             3057..3749
                     /locus_tag="ACHU9J_003964"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1907388.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHU9J_003965"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHU9J_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1907389.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         1437 a         1414 c         1310 g         1246 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttctt
//
LOCUS       DAZPJP010000245         5351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPJP010000245 DAZPJP010000000
VERSION     DAZPJP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.248"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHU9J_003966"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHU9J_003966"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1907390.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHU9J_003967"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHU9J_003967"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1907391.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHU9J_003968"
     CDS             complement(2698..3390)
                     /locus_tag="ACHU9J_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1907392.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHU9J_003969"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHU9J_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1907393.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1382 c         1267 g         1334 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc c
//
LOCUS       DAZPJP010000246         5330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPJP010000246 DAZPJP010000000
VERSION     DAZPJP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.249"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            119..1477
                     /gene="mltD"
                     /locus_tag="ACHU9J_003970"
     CDS             119..1477
                     /gene="mltD"
                     /locus_tag="ACHU9J_003970"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1907394.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1526..2149)
                     /locus_tag="ACHU9J_003971"
     CDS             complement(1526..2149)
                     /locus_tag="ACHU9J_003971"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1907395.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2153..2953)
                     /locus_tag="ACHU9J_003972"
     CDS             complement(2153..2953)
                     /locus_tag="ACHU9J_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1907396.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3194..4108
                     /gene="yafC"
                     /locus_tag="ACHU9J_003973"
     CDS             3194..4108
                     /gene="yafC"
                     /locus_tag="ACHU9J_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1907397.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4105..4908)
                     /gene="dkgB"
                     /locus_tag="ACHU9J_003974"
     CDS             complement(4105..4908)
                     /gene="dkgB"
                     /locus_tag="ACHU9J_003974"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1907398.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5071..5147)
                     /locus_tag="ACHU9J_003975"
     tRNA            complement(5071..5147)
                     /locus_tag="ACHU9J_003975"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5111..5113),aa:Asp,seq:gtc)
BASE COUNT         1396 a         1291 c         1320 g         1323 t
ORIGIN      
        1 ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg
       61 tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat
      121 gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa
      181 cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac
      241 aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc
      301 tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa
      361 acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta
      421 tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact
      481 acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat
      541 ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga
      601 cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa
      661 caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg
      721 ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt
      781 accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct
      841 caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc
      901 gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc
      961 cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg
     1021 gccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctcgcttc
     1081 aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg
     1141 tgtttacacc gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag
     1201 caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag
     1261 tttgacgatt ggtgcaggca gtagcgcaca gcggttggca aacaacagcg atagcattac
     1321 gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat
     1381 caaagatgtg atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac
     1441 gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaaggca
     1501 ccgattcccc cggtgccttt tttatttatg ccgctttatg tgcttctacc atgatgatat
     1561 cactggtgaa agagccatca gtctgcaagg aaaaatacgt tctcacctct gtcgatgcgc
     1621 tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcacgaccc
     1681 atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt
     1741 cattgattaa cgtcaaccac gcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta
     1801 atgcttctac cgcctgtaac cagatgtcgc gcactgggtg acccggagac attacatcca
     1861 tcacaatcag cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa
     1921 catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa
     1981 atggcagact tgcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg
     2041 cagcttgtgc cacgacatcc agcatgtggg cagataagtc atacgccacc accgcgctca
     2101 cgttttgcgc agcgccaaac gttggtctgc cctgcggcag acctgacata ccttatttat
     2161 caggcttgcc gggactgaat tcaacgagta gcggattgtg atcggaagcg cgcgtaacca
     2221 gtacagaagc ttcactgacg ttcagaccac ggtagaaaac aaaatcgagc gggcgaccaa
     2281 acgcccgacg gcgctgatca tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa
     2341 agcgatataa cgcgttcatc cttctacggc tccaggcatt gaaatctccc gccataatga
     2401 ccgggccgct gtggtgagct atctgatcac caataggaag taactgctta ctatagacat
     2461 ccacgcccag actgaagttg acggcgtgta tattaaccac catcaacagg cgggtgtcag
     2521 gtaatggata gaccgtcacc agtgccgact tcgccagacg caaaatgggt tctcgttcac
     2581 gtaacgggca gcaatacact ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt
     2641 gtggcagcac gaaagcgggt acctgatcgg cggcaagata gttagcggtc gcaaactgta
     2701 ccaactctgg cgttgtttgc gcttcctgca ataacaccag atgtgcgtct ttgccgtaat
     2761 tctttaatac cgacaaccat tcagcgcgtt gctgtttgta tatgttccac accaggatcc
     2821 gaatacgctc ttcggtactt aacggttctc cgggtggtaa tgcctggccg atgctcgcaa
     2881 aagaccccgg cggtaagatc ctctccgcag gttgtccggc aacatagcgc atggcatagg
     2941 tgttttttcg cacttttgac tttgaaacct ctattacgtc aacccttccg cactcgggag
     3001 ggttctactg ttatagggat tttagctcac tctttcaacg agcaattgta tattgttatg
     3061 taagcaagtg cttacaccgg tcagaaattt gctcattatt gagaacatta aatcggatga
     3121 catatcacgc atcctgatgg tttaatgaca aataaattca aaatatctca tctattttgc
     3181 aacaggagca aaaatgaaag ccacgtcgga agaactcgcc atttttgttt cggtcgtcga
     3241 aagcggcagc tttagccggg cagcggaaca attagggcaa gcaaactcag cggtaagccg
     3301 ggcggtgaaa aagctggaaa tgaaacttgg cgttagcctg cttaatcgga ccacgcggca
     3361 acttagcctg acggaagaag gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga
     3421 gatggcagcg gcagaatcag aaattatgga gacgcgtaat acaccgcgtg gactgttacg
     3481 gatcgatgcc gcaactccag tggtgctgca ctttctgatg ccgttaatta agcctttccg
     3541 tgaacgctat ccggaagtca ctttatcgct agtctcctcc gaaacgatta ttaatttgat
     3601 cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta acggactcca gcttacgtgc
     3661 caggccgtta tttaacagtt atcgaaaaat tatcgcctcc cccgattata tttcccgcta
     3721 cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt tgcctgggat tcactgaacc
     3781 cgcttccctc aatacctggc cgatagcctg tagtgatgga caattacatg aggtgaagta
     3841 cggtttgtca tccaatagtg gggaaacact gaaacagctt tgcctgagtg gcaacgggat
     3901 tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct cgcggagaat tggtggagtt
     3961 aatggcagat aaagtgttgc cagtggaaat gccattcagt gccgtctatt acagcgaccg
     4021 tgcggtaagt acgcgcatcc gggcttttat cgatttcctt agcgagcatg taaaaacagc
     4081 tcccggagga gctgtcagag aggcttaatc ccattcagga gccagacctt ccgggctaac
     4141 caggcggtcg ttgcaatcca gtgcggcgat cgctttttta tcttcggcat caagctgtaa
     4201 attttgtgcc ttaagattac tttccaggtt tttacgttta gtagaagaag gaattactga
     4261 gtaaccttcc cccatagccc acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc
     4321 tgcaatacga gcaataacct catctttcag tgccttacca taagccagcg tcatatagga
     4381 agtgatatga ataccgtgct gtttagccca ggcaaccact ttacggtttt gcagataagg
     4441 agagagttca atctggttgg tagcgatgtt ttcagcgcca acagcagcaa tcgctttttc
     4501 catcaacggg atcgtgaagt tggaaatacc gatctcacgt gtcagacctt gttttttggc
     4561 ctccagcagc gcctgcataa actcttcaac agagacttca tcgtttggtg acggccagtg
     4621 gattagcgtc agatcaacat aatcggtacg caatttttgc aggctctctt ttagactcgg
     4681 gatcaatttg tctttgctga gattttcaat ccagatttta gtggtgatgt agagttcatg
     4741 acgtggcacg ccactttctg caatcgcctg acctactgcg gcttcgttat catagatttg
     4801 tgcagtatca attgcgcgat aaccaagttc aagcgccgtt ttcacagatg aaataacaac
     4861 gtcgtctttg agacggaaag tacctaaacc aaatgcaggg atagccataa tatgcctctt
     4921 ttaatgctat tttcgttaac tgaggtgatt atgggcttca gactgttgcg gaaaaagagc
     4981 ataaaatgca gaggattttt gcgattctgg caataataga tatacagacg aaaaaaaacc
     5041 tcgagcgtta actcgaggct tcttaataag tggcggaacg gacgggactc gaacccgcga
     5101 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc gaattctttt
     5161 acaaccaccg gttttatgac cggcttactg cttaatttga tgcctggcag tttatgaatc
     5221 acttcgtgat tcacccttcg ggccgttgct tcgcaacgtt caaaagcttt cgcttttgtc
     5281 ctactctctt atcgagtagt gaactgcctc aaaggtcagt gccattcttc
//
LOCUS       DAZPJP010000247         5322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPJP010000247 DAZPJP010000000
VERSION     DAZPJP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.250"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            227..829
                     /locus_tag="ACHU9J_003976"
     CDS             227..829
                     /locus_tag="ACHU9J_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR1907399.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGISNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRTQAQQENISGQ"
     gene            885..1379
                     /locus_tag="ACHU9J_003977"
     CDS             885..1379
                     /locus_tag="ACHU9J_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907400.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLNLMDLAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(1456..2304)
                     /locus_tag="ACHU9J_003978"
     CDS             complement(1456..2304)
                     /locus_tag="ACHU9J_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1907401.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(2344..2622)
                     /gene="yacB"
                     /locus_tag="ACHU9J_003979"
     CDS             complement(2344..2622)
                     /gene="yacB"
                     /locus_tag="ACHU9J_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFR1907402.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QAYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(2622..2891)
                     /gene="yacA"
                     /locus_tag="ACHU9J_003980"
     CDS             complement(2622..2891)
                     /gene="yacA"
                     /locus_tag="ACHU9J_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR1907403.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(3798..4829)
                     /gene="repA"
                     /locus_tag="ACHU9J_003981"
     CDS             complement(3798..4829)
                     /gene="repA"
                     /locus_tag="ACHU9J_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR1907404.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRQAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1263 a         1377 c         1303 g         1379 t
ORIGIN      
        1 tttttattta tatcatgaca acattaaagt gagagaatat atcaaccatg tttctgtcag
       61 ggttcagtag aatatcaatt cgactgtcac gccataactg taactacctc gagtataaat
      121 attcagagaa tggacttatt agccatgaaa aatcacctga tgggaaattt actaatttat
      181 ggattgtgat attattaaag agcaaaacca gccagccgag gtatttatgg gaaaaaatca
      241 ggaagaaagg aaaactcccg tcattgtggt gaaaaaacgt cgtacttttt cgccaccgtc
      301 cttatccgaa aaaacagata ttatcgcacc tgtatttact gaacagacgg cagaatcagc
      361 ccccgccgga ataaattcat ctgccgtcga aacacatatc cccgaagcgc cagccaggaa
      421 gaagaaaaag aaaagacacc gttttccacg tccctcccac tggacccgtg agtatactca
      481 cgagtgcgtg gaaaaaataa aggccctgtt tcctcatctt cgtgctgaag gtggtggatt
      541 tattcccctg aaaattggca taagcaatga tatttcggca tttctggcag aacacccgga
      601 gacagaactg accatggacg aatggctctg tgcagtgtcc tgcatcacat cccgccgcgt
      661 atatctgcag cggacagctg tcgccggcgt cccacgttat ggcctggatg gtcaccccaa
      721 agggcaggtc tcagactctg aagcacagag tgccggtcgc agactggcga cattagaaca
      781 aaagtggctc aggacgcagg cacagcagga gaatatttct gggcaataaa cactcctgat
      841 aacaggtgga cattacgtat cagaaaaaat gggaagagaa tgttatgtgt gattcagaag
      901 cggagaagat tttattcatc ctgaataaac atgaacgggc atacagacgc ctgatccccg
      961 tatatatttt tttagctata gtattatgga ttttacctat tctcctgatt gtatacctgt
     1021 atttctgtat agttgacaat gagtttctca gaatcggttt ctgtattgca tggacagcat
     1081 actactttct ttcgcttttc aagtattttg taaaatactt ctttcctctc gatcgtgaag
     1141 atttgcttaa cctgatggat ctcgccaaag attctcctga tgcgaaacag gagcttcttg
     1201 agcgtctttt gtccggaaaa agaatgaccg ggcgggatga aatggatatc aagcgcattc
     1261 taaaaacaaa ggaagcactt gataacatca ggaattttat tgataaacag cagttaacga
     1321 atagtgatac tccgaagaaa agcacggtcc caaatgaaga taaaaaaggc ataagatgat
     1381 gatcacagga aatggaataa acactgtaac tgtcaatgga aaagtgaaac tgattgcggt
     1441 atacaggcca ggcggtcaga ttttcagccg tgcctgttcc tgcagcagtt ccagatgata
     1501 tgcagcaata gcgtttacca cccctgccgt catccgtgcg tcggcgatcg ctgagtgtgc
     1561 ctgcccttcc caggtcagtc cggcctggct ggcagcacag gccagtgaaa tcgtgccata
     1621 gcggttggtg gccccataat atcctgcagc cagctccatc gcacaatata ccgtcagttc
     1681 ttccagccag tcagccggat cgctatgtgc ggcagcagtc tgtttcagaa tgcggatgtc
     1741 gaaccgggca ttaaagataa ttactggtcg gtccccgatt gcatgacgca gttgccgcgc
     1801 cacatctgtc catgatggtg cgccgcagag agcctgctcg ctgataccat gcacagcggc
     1861 ggcctgcgcc ccgatggcga ccgtgggctt cagcctggtt tcaaatacca cctgaccggc
     1921 cgcatcggtc aggccgattt ccagggcttc cgcggtatta tccagtcctg tcgtttcagt
     1981 atccagaaag agcggtgcca gactcagcca gtcatatgcc tgccgtgcca tccggccact
     2041 ggtgctgtta agtctggata aaacggaaag tcgctggcca gccagctgtt gtttactcgt
     2101 cagagggcgt ttctcacgca tcggaacaca gtctgcaatt cggtatacag cgaacctgcc
     2161 accataagtg tttttatacc acttaaccgg ctcagcaccg ggggctggtt tcattctgaa
     2221 ttcatcgcgc aaacgtcctt tcgagaaaca cctgtcttcg gcaatcgcct gcgcacgtct
     2281 ggtggccagc tcatcattat tcatggttat tgcatctcct ttccttatat ccggacagcc
     2341 cctctatggc cacttctgtg cagtatgcaa tacgcgcagg atatacactt ttccccactg
     2401 gctgtcgacc tcataaacca gaacaaaatg cgggtgaata accagttccc gggtaccttc
     2461 cacacgaccg ttgcgacctt tatacgcctg cccggccagg ctactggctg aataaccaat
     2521 ccgttcgtcc agctcaatgg cggccatcag gttctgctcc gcaatgtact cacgaatgcg
     2581 cttcctgtcc tggctggcca gcatggtcca gaatatctcc atcatttacc ggcaaccttc
     2641 ctgctcattt catcccgcca tgcagcagcg gaagattcga ccatgtcatg gggaagcaca
     2701 tcaccgcgtt cgagttgctg acgtcctgcc tcaacctggt cacggaacca ggtattatgc
     2761 tgccgttcaa cggtctggcg cataaaatcc cggattaact gagagccatt acggtccatg
     2821 ctttttgcag cggccataaa agcatccttc agttcagcgt cgattcttaa actcatatta
     2881 acctgtgcca tttatcacct cgtcattact acgtatatac acagtatata acgattcaga
     2941 tatcgtcacc aggatatgcc gcgccagcgg catggaaggc ggcactcagt tgttgcatat
     3001 gataccggag taaaaccgcc gaagcccggc gtaagccggt actgattgat agatttcgcc
     3061 tatcccattc ccaccctgct aagccatacc cgctttcagc catgatagag cctctgtgcg
     3121 cggtcggagt ggccccgacg agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg
     3181 gccgtcaggg ccgcttacga gcgtgtactc gatacgtcca gccagaagac tgacagcgat
     3241 gacaatgtag ttacaacatt cataattaaa agcgatgttg ttcccgccct tcgggcgggg
     3301 gcggcctcgc ttttcagtta tgaggggtgg gcacggtgct gcgtacgtgt ggtgtttttt
     3361 attcctgatg ttttcgcggt ggtttcagaa cggtgaggtt cggggcccgc gcgttagcgt
     3421 gggaggtgct tttagattct gagcggcgcg gccttggccg ctgcaagcga aggcctcttt
     3481 gtgtttgttc acagtggttt cagagataaa gattttccct gcggcgaagc aacgcagggg
     3541 attaaataat ggggttaaga ctatattgca ggtttgctgt ttcaggtttg cagcttctta
     3601 aagttctttg tttgcggatt tatgtggata actgaaacaa cgtggccacg ttctgatgac
     3661 tgatgcgtgg tttaatgggc aaatatgccc ctctgagggg atttaaggtg attactgcgg
     3721 attttgggct gagcggaacc agcgccgaaa gccccggagg ggctggcgat aattcagtgc
     3781 tgagggaatg gtgctgtcta gtgcggtggc ggggcagcca gcgtcagttc aagctgacgc
     3841 aactccctga tggcctgtcg cttcagatgg tcatcggaac aaaaataggc ttcatccggc
     3901 ggcagacgct tcctgagata ctctgccatc tcatgaatct gctggtcggc aggcagtttc
     3961 ttcaggcgtc tggcgcgctt actggctgct gctttcgcac gacggtgttt cagcgcatcc
     4021 tgactgcgct gcagatacca gcgttttctg gccgcatgta cggagatatc ttcatcctca
     4081 cgcagaacac cttcccgaat gagctgctgg cggatttcac tttcacgcag tcgtttctgc
     4141 tgctgttcgt gaagttttac catgtcgacg cccagcatct gccagcctgc cggtgttatc
     4201 cagacgtacc gtggcagacg ctggcgggtt tcacggtccc acagggtttc ctctgaaaca
     4261 cccagcacac caaaacgtac ctgttcggcc agcaaacggg aaaggcggga gaccgtcact
     4321 tccagttccg gaataacctt ccccttgctg tctttcggac tgatttctct ggccagacgg
     4381 gaaacactca tgcctacggt atgtgtgccc gcatcactga agctgaccag aaccggccag
     4441 acggcatcga ggaggcgttg tttttccgga cggaaagcat atttgcgacc ggcatacgtt
     4501 cgtttctgca cgtaaaaagg atgcagagac atgtgcatgc gcaggctctg ctcgccggtc
     4561 accgggtcct ttttcaccag acggttgtag gcgtgcccga gctgcccgct cagcgcctta
     4621 tagcgctcag gacgatacca ggacggattt tcgcatttgg gtttggtgga gtgctcacca
     4681 cgacgacgct tcgtgcgctt cctttccggt tcaaccagga aagtggtcgc ccttcccgct
     4741 tcatagtctt cccagaaacg gatctgctct tcgggtgcta actgactcca gtgtactgcg
     4801 ttatatggag tattcttcag acctgccaca agctgtcgct tatggcggag cgattgtgcc
     4861 gtgtatttat acactgcaca gggttgaatc tctggtacgg tttcattata ctttacccgt
     4921 attctgtgag gcccccacta tctttcgtcg ttccgccaag ttcgtaagaa agatagtggg
     4981 ggttttcctt ttatggcttc cggaacatct tcagtcttct gacttactgc ccgtacatgc
     5041 catctctcct gaatgcctta tggcgcattc tgccgtccgg ctttcgccgc tttgctccac
     5101 agagtgccac cctctgcaca gctaataaca gccgaaatat acccgcttta cgttaatttg
     5161 aaaagcatta caacgatctc ttctcttcca gtgcctcctc actgatacct ctaataagtt
     5221 aatcttgacc tgcgtcaaaa aaataaaatc tatttttaaa agcagatttt tctgctttag
     5281 atctttcttt tggattaaaa gatcgacttt tgtcctactc ac
//
LOCUS       DAZPJP010000248         5271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPJP010000248 DAZPJP010000000
VERSION     DAZPJP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.251"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..747
                     /gene="ydeO"
                     /locus_tag="ACHU9J_003982"
     CDS             <1..747
                     /gene="ydeO"
                     /locus_tag="ACHU9J_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1907405.1"
                     /translation="SVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFK
                     EIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFS
                     ELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISES
                     LLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHF
                     GNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            1148..2830
                     /locus_tag="ACHU9J_003983"
     CDS             1148..2830
                     /locus_tag="ACHU9J_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1907406.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2882..4039
                     /locus_tag="ACHU9J_003984"
     CDS             2882..4039
                     /locus_tag="ACHU9J_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1907407.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4005..4127)
                     /locus_tag="ACHU9J_003985"
     CDS             complement(4005..4127)
                     /locus_tag="ACHU9J_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907408.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            4336..>5271
                     /locus_tag="ACHU9J_003986"
     CDS             4336..>5271
                     /locus_tag="ACHU9J_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR1907409.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLM"
BASE COUNT         1594 a         1095 c         1036 g         1546 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttta aagagatcgg atttactgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat cttaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tccggaaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc acatgggtat gaatacgggc
      721 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgct
      781 gcgtaagaat taaatggggc agccattccc tgccccgcgt tgtttttagg cgatatattt
      841 attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc tgttgcagat
      901 tatttatgat ctcaattcgg cattttgtca gtaaaatgca ataatttatt aaatatcaat
      961 aaattagttg tttatcggcg agaaattact taatagaaca gaaagtaatg tcaacgcttt
     1021 atggactgtt atttcccttt ttttagctaa atctgctatc tctttatgtg actaacttca
     1081 cttacatcca cttatttctc ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg
     1141 aacatttatg aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact
     1201 ggcatctggt atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac
     1261 caaaacaaac gttgctttct cagattttac gccgactgaa tacagtacca aaggaaagcc
     1321 aaatattatc gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg
     1381 atcttttgac ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat
     1441 agataaagcc attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga
     1501 aggcgtacgt tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc
     1561 aataatgacc ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga
     1621 tggtattccg ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac
     1681 tgcagcagta ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa
     1741 acaaacgcgt gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca
     1801 aaaccgtggc tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc
     1861 cccttcactg ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt
     1921 aaccgatgag gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct
     1981 ttacctggct tataatgctc cgcacctacc aaatgataat cctgcaccgg atcaatatca
     2041 gaagcaattt aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt
     2101 tgatcagggt gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac
     2161 aattattctc tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg
     2221 ggcgcaaaaa ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat
     2281 gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt
     2341 ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg
     2401 tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gcgccacata aaaatctgac
     2461 ctggataacc tcttattctc actggtttga tgaggaaaat attccattct gggataatta
     2521 ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt
     2581 aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa
     2641 caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc
     2701 caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca
     2761 gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga
     2821 agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc
     2881 gatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt
     2941 ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa
     3001 agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta cctggcaagg
     3061 cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg
     3121 ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa
     3181 tgaatggtgt gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc
     3241 ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt
     3301 gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat
     3361 taataacgtt aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag
     3421 taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg
     3481 tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa
     3541 gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg
     3601 tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt gtattttcca
     3661 ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca
     3721 ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa
     3781 cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata
     3841 taaacctatc tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac
     3901 tgtttcttat ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa
     3961 cgccatggtt gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa
     4021 acaaatggag aataattaat aatgctctct tttaactcca ttcttagcct ccttcttatg
     4081 accttaacaa ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct
     4141 tgcgaaaatc ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa
     4201 tagcataacc ataaccataa ccactgaaaa ttaaaaattt ctatatattt catattgtta
     4261 aatgatactt aaataaattt catcagtaaa tcattgccat agataatcat tggatggcac
     4321 aatcaatttt cgctaatgat aaccattccc attacgtttt atatgttgat agcaaagtat
     4381 ctctgtctgc tgaagccctt ttggctgcgt aagaataata aaacttcagt gttgttaatc
     4441 attattatcc tcgcaatgat ccttggcgtg gttaaaattc aggtctggtt aaacgactgg
     4501 aataatgatt ttttcaatgc gctaagccag aaagaaaccg ataagctttg gcagcttgtt
     4561 ctttggttcc ctgccctgct ggggatcttt gtattaatat ccgtaaacaa aacatggtta
     4621 attaaactac tgactattcg ctggcgcgaa tggttaacag attactacct taatcgctgg
     4681 tttgcagata aaaactacta cttcacgcag atctacggcg agcataaaaa tacagataac
     4741 cctgaccaac gtatcgctga agacattctc ttactgatca gcaaaacgct cagtctttca
     4801 tttggcttca tccagtcact tagcatgctg atcaccttta ccgttattct ttggcaaagc
     4861 gcgggtacgc tctcattcac tgttggtgga acagaatgga atatccaggg gtatatggtc
     4921 tataccgtcg tgctcattgt gatcggtgga actttattta cccataaggt gggtaaacgt
     4981 attcgtccgc ttaatgtgga aaaacaacgt agcgaagcga catttcgaac taatcttgtg
     5041 cagcataata agcagacaga actgattgcg ctaagcaatg ctgagtctct acaacgccag
     5101 gagttgcgcg acaattttca taccattaaa gagaactggc atcgtttaat gaatcgtcag
     5161 cggtggcttg attactggca aaatatttat tcgcgctcgc ttagcgttct tccctacttt
     5221 ctgttattgc cgcagtttat tagcgggcag atcaatctgg gcggactgat g
//
LOCUS       DAZPJP010000249         5228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPJP010000249 DAZPJP010000000
VERSION     DAZPJP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.252"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            70..516
                     /gene="hpaR"
                     /locus_tag="ACHU9J_003987"
     CDS             70..516
                     /gene="hpaR"
                     /locus_tag="ACHU9J_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1907410.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            635..2290
                     /gene="tsr"
                     /locus_tag="ACHU9J_003988"
                     /pseudo
     CDS             635..2290
                     /gene="tsr"
                     /locus_tag="ACHU9J_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(2339..3700)
                     /locus_tag="ACHU9J_003989"
     CDS             complement(2339..3700)
                     /locus_tag="ACHU9J_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907411.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLMRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(3915..4829)
                     /locus_tag="ACHU9J_003990"
     CDS             complement(3915..4829)
                     /locus_tag="ACHU9J_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1907412.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            4968..>5228
                     /gene="lgoD"
                     /locus_tag="ACHU9J_003991"
     CDS             4968..>5228
                     /gene="lgoD"
                     /locus_tag="ACHU9J_003991"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1907413.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVI"
BASE COUNT         1377 a         1216 c         1390 g         1245 t
ORIGIN      
        1 cgcagtcccg gtaacgtgac gattaactgt ttctatatta atgattttga atttttcagg
       61 ggaaagataa tgcacgactc actaaccatt gcgttgctcc aggcgcgaga agcggcgatg
      121 agttattttc gcccaatcgt taaacggcat aatttgactg agcaacagtg gcgcatcgtg
      181 cgtattctgg cggaaagccc atcaatggat tttcacgatc tggcgtatcg cgcctgcatt
      241 ttgcgcccaa gcctgaccgg gatcctcacg cggatggaac gcgacggttt agtgttgcga
      301 ttaaagccga tcaatgatca gcggaagctc tatatctcgc taaccaaaga ggggcaggcg
      361 ctgtataacc gcgctcagac acagattgaa gaagcctatc ggcagattga agctcagttt
      421 actgccgaga aaatgcaaca gttaacccat ctgttagagg aatttattgc tctgggcaat
      481 tcccgacaag aagccatccc gggcgataat gaataacttt ttagtatctg ataaccagcg
      541 cctggttttt ccatggatgg tgggttacac cttttcataa agtttttgct ttccaggccg
      601 aaaatcttgc atcggtccac aggaaagaga aaccatgtta aaacgtatca agatcgtgac
      661 cagcttactg ctggttttgg ccgtttttgg ccttttacaa ctgacatcag gcggtctgtt
      721 ctttaatgcc ttaaagaatg acaaagaaaa tttcactgtt ttacaaacca ttcgccagca
      781 gcaatccacg ctgaatggca gctgggtcgc gttgttgcag acgcgtaaca ccctcaaccg
      841 cgcgggtatc cgctacatga tggatcagaa caatattggt agcggttcaa ccgttgctga
      901 gctgatgcag agtgccagta tttcgctgaa acaggcggaa aaaaactggg cggattacga
      961 agcgttgccg cgtgacccgc gtcagagcac cgccgcagcg gcagagatca aacgtaatta
     1021 cgatatttat cacaatgcgc tggcggagct gatccaactg ttaggtgcag gcaaaatcaa
     1081 cgagttcttt gatcagccga cccagggata tcaggacggt ttcgagaagc agtatgtggc
     1141 ttacatggag caaaacgatc ggctctatga tatcgccgtc agcgataaca atgcctccta
     1201 cagccaggcg atgtggattc tggtgggcgt gatgatcgtc gtactggcgg tcatcttcgc
     1261 cgtctggttc ggtattaaag cctcgctggt agcgccaatg aatcgcctga ttgacagcat
     1321 tcgtcatatt gcaggcggcg atctggtgaa accgattgag gtggatggct ctaatgagat
     1381 ggggcaactg gcagagagtt tgcgccatat gcagggagag ctgatgcgta ccgtcggtga
     1441 tgtgcgcaac ggggccaatg ccatctatag cggtgccagc gaaatcgcca ccggcaataa
     1501 cgatctctct tcgcgcaccg agcaacaggc cgcttcgctg gaagagacgg cagccagcat
     1561 ggagtaactg accgcaacgg tgaagcagaa cgccgaaaat gcgcgccagg ccagccatct
     1621 ggcgttaagt gcttctgaaa cggcgcaacg cggcggcaaa gtggtagata acgtggtgca
     1681 gactatgcgc gatatctcca ccagttcgca gaaaatcgcc gatattatca gcgtaattga
     1741 cggcattgcc ttccagacca atattctggc tttgaacgcg gcggttgagg ctgcgcgtgc
     1801 gggtgagcaa gggcgcggtt ttgcggtggt cgcgggagaa gtgcgtaatc tggcccagcg
     1861 cagcgcccag gcggctcgtg aaattaaaag cctgattgaa gactcggtgg ggaaagtgga
     1921 tgttggctct acgctggtcg aaagcgccgg ggaaacaatg gcggagattg tcagcgccgt
     1981 gacccgcgtg acggacatta tgggcgaaat tgcttctgct tctgatgagc agagccgtgg
     2041 tatcgatcag gttggcttag cggttgctga gatggaccgg gtaactcaac agaacgccgc
     2101 gctggtggaa gagtctgccg ctgccgccgc cgcgctggaa gagcaggcca gtcgcctgac
     2161 cgaagcagtg gcagtgttcc ggattcagca acagcagcgt gaaacatcgg ctgtggtaaa
     2221 aaccgtgacg ccagctgcgc cgcgtaaaat ggccgtggca gatagcgagg agaactggga
     2281 aacattttaa tcgccatgaa aatgcccgat aagcaaaatg ttatcgggca taaggagatt
     2341 aatctttacg tgggtcgttg atcggctgac gaaccaggaa gatgtacgcc agtgcccccg
     2401 ctgcggtgac gcaaccgcag atgattagtg ccagacggaa tgagtgggtg gtatcaacaa
     2461 taaaaccagt aatgatcggc gcaaaagagg cgcagatgaa gctggcaaag ttctggatac
     2521 tgcccaccga cgcagtcatg cgagaagcaa ctgcgacgtg gatcaagccc cagcaggatg
     2581 ttccggcaaa gtgaatacag aacagtgcca tgccaatcag cagaaccgct gtcatggatg
     2641 ttgtcgcttg cggtacgacc agcgtaaagg cggcagaaca gaacatcccg gcaataatgc
     2701 agatcttacg gcttttaatc ggagccattc cccctttgac cagccagtca gtaacgtaac
     2761 cgttgaccag catcccggca gccccaaaca ggaaagggat agccgccatc aaccctgtgc
     2821 tttttaaatc caggttatag gctgtttgca ggtaaccagg aagccaggcc agatacagcc
     2881 acgcagtgta gttgatgcca ctgaatccga gcatcattcc ccacattgta cggttacgga
     2941 acaggctgcg ccattcggca aaactgagcg gatctcggcg ggcattgacg ctacctgcat
     3001 tgagataagc ttgttcaacg gcagtcagtt ctacgtgctc gcggttgcga tagagcatat
     3061 accagccgat ggcgagaaaa atccccagta caccaatggt aataaacatc ccgcgccagc
     3121 ccatcaccag catcatcgcc gccagaatcg gtgggcttac ggcaacgcca atggtagaag
     3181 ctgcgttgaa gaagcccatc gggcgtccgc gctctttgat gttgaaccag tcgttaatga
     3241 cttttacacc gcatgggttc atcggcgctt caccaatccc cataccgata cgcatcaaca
     3301 cgaactgcgt aaagttgtgc accatgccag acattgcctg gaacagtgac cagaagaaca
     3361 tccccagtcc cagcatcagg cgtgggcctt tacgatccaa tagtgggccg caaggaagtt
     3421 gcgcaatccc gtaagcgagt gaaaacacgg agagcaaagc gccgatttcg gtggcactta
     3481 atcccaattc ttcacgaatc gttaaatttg ctaccgacag cgaactgcgg tcgagataat
     3541 tgattaccgc cgcaaaaaat aataacaaca tggcggtggt ttgaatgcgt ttaattcggg
     3601 tactgcgttg aactaatcca tctggtggca cgggaatatc tgccgacgaa gatggagtaa
     3661 atgaagaacg cggatcgagg gtgatatttt ctttttccac gccggactcc tgtatttata
     3721 attttaattg tttacgtaat tgtgaattac gcaaggttga cattaacggc aggttaaagt
     3781 tttaggactt tactaccgct cacaaaaaca tttttagtcg actgattgtg agcataggta
     3841 agcgggaatt tcccggtatt tatcttttga taaaaccgtg cacccgctaa tagtttgctg
     3901 tttttaatcc gtacttaatg agcataacgc gtgttctcat taatggatcg aatcattgat
     3961 tgtttagctg aattcaaatg gttacggagc gcaagggtgg catcaaggtc actgcggcaa
     4021 atcagagcgc tgagaatggt catatgctca tcgacggcaa tgatattgcg ctgtttaaga
     4081 tcgctttcgt cccattgata gtgaaaatga aagatgactg agatgatctc aagcgattga
     4141 tcaaaaaaga tattgtcggc agctgaaagc agtaaggagt gaaaatctct gtcaagttgc
     4201 gagaacatgc gaaagctatt gccgatgttg tcacgcagta gccgatgacg ttcgagcatg
     4261 gttttcgcct gcagccagcg gggatcatga tcaggcaggt tgagaaagtg ctgaagtgaa
     4321 tgtgtttcga gcatttcgcg tagctcaaac agctgctcgg cgtaggattg atcgaattgc
     4381 ttcatgctcc actggccgcg cttttcgctg tgaataagat tataacggcc gaattttaaa
     4441 agatattctc ttactacaac cgggctgaca cccgcggccc gagccagttg cagttcagaa
     4501 aacgtttccc ctgggcgtaa ctggcgctgg tttatcatcg tgaaaaaagc ctgctcaaac
     4561 actttgtttt gttcactcat tgaagcggta gtacaggcga aaccgtcatc atggtcaggt
     4621 ttacgcgcaa taacatagtc gtttccaacc tgcgtcaaga cgccgcattc gcgtaagtgg
     4681 ctgagaatat gacgcactgt ggtgcggctg atgttgtaca tttccgccag cgcactttgc
     4741 gatggaagtg gggaagggat atgaccccgc gccatatcat caatgacctg gttaatcaca
     4801 ttgtggcgta aattttgtga acgactcata ttttcctcct tgtgagtcca ttaaaacccg
     4861 atttaaaaca tttttatgcg tgtgttttca caaaacagat tttccgttcc gctgccattt
     4921 ttcattttca ttgatatcta aggcaacaaa aaatggagag ccgaatcatg tctacgatga
     4981 atgttttaat ttgccagcag ccgaaagaat tagtctggaa acaacgcgag atacctattc
     5041 cgggtgacaa tgaagcatta ataaaaatta agtctgtcgg gatttgcggt accgatattc
     5101 atgcctgggg tggaaatcaa ccatttttta gttatccacg tgttttaggc catgaaatat
     5161 gtggggagat tgttgggctg ggtaaaaata ttgctgatct taaaaatggt cagcaagttg
     5221 ctgtgatc
//
LOCUS       DAZPJP010000250         5198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPJP010000250 DAZPJP010000000
VERSION     DAZPJP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.253"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHU9J_003992"
     CDS             <1..69
                     /locus_tag="ACHU9J_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907414.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHU9J_003993"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHU9J_003993"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1907415.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHU9J_003994"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHU9J_003994"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1907416.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHU9J_003995"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHU9J_003995"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1907417.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHU9J_003996"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHU9J_003996"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1907418.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHU9J_003997"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHU9J_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1907419.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1114 a         1387 c         1382 g         1315 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaagcg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatc
//
LOCUS       DAZPJP010000251         5077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPJP010000251 DAZPJP010000000
VERSION     DAZPJP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.254"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHU9J_003998"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHU9J_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHU9J_003999"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHU9J_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR1907420.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHU9J_004000"
     CDS             complement(1327..3060)
                     /locus_tag="ACHU9J_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1907421.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHU9J_004001"
     CDS             complement(3057..3551)
                     /locus_tag="ACHU9J_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1907422.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHU9J_004002"
     CDS             complement(3673..4023)
                     /locus_tag="ACHU9J_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1907423.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHU9J_004003"
     CDS             complement(4066..4530)
                     /locus_tag="ACHU9J_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1907424.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHU9J_004004"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHU9J_004004"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR1907425.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT         1103 a         1483 c         1262 g         1229 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatt
//
LOCUS       DAZPJP010000252         5015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPJP010000252 DAZPJP010000000
VERSION     DAZPJP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.255"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHU9J_004005"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHU9J_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHU9J_004006"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHU9J_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHU9J_004007"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHU9J_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHU9J_004008"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHU9J_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1907426.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHU9J_004009"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHU9J_004009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHU9J_004010"
     CDS             complement(2927..3859)
                     /locus_tag="ACHU9J_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1907427.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHU9J_004011"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHU9J_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1907428.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1219 a         1165 c         1173 g         1458 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgt
//
LOCUS       DAZPJP010000253         4964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPJP010000253 DAZPJP010000000
VERSION     DAZPJP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.256"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHU9J_004012"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHU9J_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1907429.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHU9J_004013"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHU9J_004013"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1907430.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHU9J_004014"
     CDS             2475..4124
                     /locus_tag="ACHU9J_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1907431.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..>4964)
                     /locus_tag="ACHU9J_004015"
     CDS             complement(4278..>4964)
                     /locus_tag="ACHU9J_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1907432.1"
                     /translation="ILDDSNFAQIKMADGTLNACSVMHVQFYNAAMNRANIKNTFLDY
                     SNFYMAYMAEANLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINL
                     IDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRV
                     NFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMK
                     ITADIMQHAD"
BASE COUNT         1050 a         1169 c         1363 g         1382 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaat
//
LOCUS       DAZPJP010000254         4943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPJP010000254 DAZPJP010000000
VERSION     DAZPJP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.257"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            237..1181
                     /gene="leuO"
                     /locus_tag="ACHU9J_004016"
     CDS             237..1181
                     /gene="leuO"
                     /locus_tag="ACHU9J_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1907433.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSSLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1336..1380)
                     /locus_tag="ACHU9J_004017"
     CDS             complement(1336..1380)
                     /locus_tag="ACHU9J_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907434.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1499..3223
                     /gene="ilvI"
                     /locus_tag="ACHU9J_004018"
     CDS             1499..3223
                     /gene="ilvI"
                     /locus_tag="ACHU9J_004018"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1907435.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNVTVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3226..3717
                     /gene="ilvN"
                     /locus_tag="ACHU9J_004019"
     CDS             3226..3717
                     /gene="ilvN"
                     /locus_tag="ACHU9J_004019"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1907436.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3729..3815
                     /locus_tag="ACHU9J_004020"
     CDS             3729..3815
                     /locus_tag="ACHU9J_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1907437.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3897..4901
                     /gene="cra"
                     /locus_tag="ACHU9J_004021"
     CDS             3897..4901
                     /gene="cra"
                     /locus_tag="ACHU9J_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1907438.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1202 a         1118 c         1303 g         1320 t
ORIGIN      
        1 gtagttatga ttagattgtt ttcgcaacaa aaacattatg gattcttatg ctgtggtaaa
       61 tgactcattc cacggcaatg gattctgttt ttatcagaac ccgtatcttt atgttttccg
      121 aattttactc attttgcttt ttcttatttt atatgcatga taaatcatat tcttcaggat
      181 tatttctctg cattccaata agggaaaggg agttaagtgt gacagtggag ttaagtatgc
      241 cagaggtaca aacagatcat tcagagacgg cggagttaag caagccacag ctacgcatgg
      301 tcgatctcaa cttattaacc gttttcgatg ccgtgatgca ggagcaaaac attacccgtg
      361 ccgctcatgt tctgggtatg tcgcaacctg cggtcagtaa cgctgttgca cgcctgaagg
      421 tgatgtttaa tgacgagctt tttgttcgtt atggccgtgg tattcaaccg actgctcgcg
      481 catttcaact ttttggctca gttcgtcagg cattgcaact agtacaaaat gaattgcctg
      541 gttcaggttt tgaacccgcg agcagtgaac gtgtatttca tctttgtgtt tgcagctcgt
      601 tagacagcat tctgacctcg cagatttata atcacattga gcagattgcg ccaaatatac
      661 atgttatgtt caagtcttca ttaaatcaga acactgaaca tcagctgcgt tatcaggaaa
      721 cggagtttgt gattagttat gaagacttcc atcgtcctga atttaccagc gtgccattat
      781 ttaaagatga aatggtgctg gtagccagca aaaatcatcc aacaattaag ggcccgttac
      841 tgaaacatga tgtttataac gaacaacatg cggcggtttc gctcgatcgt ttcgcgtcat
      901 ttagtcaacc ttggtatgac acggtagata agcaagccag tatcgcgtat cagggcatgg
      961 caatgatgag cgtacttagc gtggtgtcgc aaacgcattt ggtcgctatt gcgccgcgtt
     1021 ggctggctga agagttcgct gaatccttag aattacaggt attaccgctg ccgttaaaac
     1081 aaaatagcag aacctgttat ctctcctggc atgaagctgc cgggcgcgat aaaggccatc
     1141 agtggatgga agagcaatta gtctcaattt gcaaacgcta actgaatgca gaataggtca
     1201 gacatgaatg tctggtttat tctgtatttt ttattgaatg tagaatttta ttctgaatgt
     1261 gtgggctctc tattttagga ttaattaaaa aaatagagaa attgctgtaa gttgtgggat
     1321 tcagccgatt tattatcaat ttaatccttc gtaatggagg attttatcgt ttcttttcac
     1381 ctttcctcct gtttattctt attaccctgc gtttatgtct ctggctgcca attgcttaag
     1441 caagatcgga cggttaatgt gttttacaca ttttttccgt caaacagtga ggcaggccat
     1501 ggagatgttg tctggagccg agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca
     1561 agtattcggt tatcccggag gcgcagtcct tgatatttac gatgcattgc ataccgtggg
     1621 tggtatcgat catgttttag ttcgtcatga gcaggcggca gtgcatatgg ccgatggtct
     1681 ggcgcgcgcg accggggaag tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa
     1741 tgcgattact ggcatcgcca ccgcttatat ggattccatt ccattagttg tcctttccgg
     1801 gcaggtagcg acctcgttga taggttacga tgcctttcag gagtgcgaca tggtggggat
     1861 ttcgcgacca gtggttaaac acagttttct ggttaagcaa acggaagaca ttccgcaggt
     1921 gctgaaaaag gctttctggc tggcggcaag tggtcgtcct ggaccagtag tcgttgattt
     1981 accgaaagat attcttaatc cggcgaacaa attaccctat gtctggccgg agtcggtcag
     2041 tatgcgttct tacaatccca ctactaccgg acataaaggg caaattaagc gcgctctgca
     2101 aacgctggta gcggcaaaaa aaccggttgt ctacgtaggc ggtggggcaa tcacggcggg
     2161 ctgccatcag cagttgaaag aaacggtgga ggcgttgaat ctgcccgttg tttcctcatt
     2221 gatggggctg ggagcgtttc cggcaacgca tcgtcaggca ctgggcatgc tgggaatgca
     2281 cggtacctac gaagccaata tgacgatgca taacgcggat gtgattttcg ccgtcggggt
     2341 acgatttgat gaccgaacga cgaacaatct ggcaaagtac tgcccaaatg tcactgttct
     2401 gcatatcgat attgatccta cttccatttc taaaaccgtg actgcggata tcccgattgt
     2461 gggggatgct cgccaggtcc tcgaacaaat gcttgaactc ttgtcgcaag aatccgccca
     2521 tcaaccactg gatgagatcc gcgactggtg gcagcaaatt gaacagtggc gcgctcgtca
     2581 gtgcctgaaa tatgacactc acagtgaaaa gattaaaccg caggcggtga tcgagactct
     2641 ttggcggttg acgaagggag acgcttacgt gacgtccgat gtcgggcagc accagatgtt
     2701 tgctgcactt tattatccat tcgacaaacc gcgtcgctgg atcaattccg gtggcctcgg
     2761 cacgatgggt tttggtttac ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac
     2821 cgtggtttgc gtcactggcg acggcagtat tcagatgaac atccaggaac tgtctaccgc
     2881 gttgcaatac gagttgcccg tactggtggt gaatctcaat aaccgctatc tggggatggt
     2941 gaagcagtgg caggacatga tctattccgg ccgtcattca caatcttata tgcaatcgct
     3001 acccgatttc gtccgtctgg cggaagccta tggtcacgtc gggatccaga tttctcatcc
     3061 gcatgagctg gaaagcaaac ttagcgaggc gctggaacag gtgcacaata atcgcctggt
     3121 gtttgttgat gttaccgtcg atggcagcga gcacgtctac ccgatgcaga ttcgcggggg
     3181 cggaatggat gaaatgtggt taagcaaaac ggagagaacc tgattatgcg ccggatatta
     3241 tcagtcttac tcgaaaatga atcaggcgcg ttatcccgcg tgattggcct tttttcccag
     3301 cgtggctaca acattgaaag cctgaccgtt gcgtcaaccg atgatccgac attatcgcgt
     3361 atgaccatcc agaccgtggg cgatgaaaaa gtacttgagc agatcgaaaa gcaattacac
     3421 aagctggtcg atgtcttgcg cgtgagtgag ttggggcagg gcgcgcatgt tgagcgggaa
     3481 atcatgctgg tgaaaattca ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg
     3541 gaaatattcc gtgggcaaat tatcgatgtc acaccctcgc tttataccgt tcaattagca
     3601 ggcaccagcg ataagcttga tgcattttta gcatcgattc gcgaggtggc gaaaattgtg
     3661 gaagttgctc gttctggtgt ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa
     3721 tgatctgaat gcgcaatttg cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag
     3781 tgggaacctg gaataaaagc agttgccgca gttaatttta tgcgcttaga tgttaatgaa
     3841 tttaacccat accagtacaa tggctatggt ttttacattt tacgcaaggg gcaattgtga
     3901 aactggatga aatcgctcgg ctggcgggag tgtcgcggac cactgcaagc tatgttatta
     3961 acggcaaagc gaagcaatac cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg
     4021 tgcgtgagca caattaccac ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac
     4081 gttctattgg tcttgtgatc cccgatctgg agaacaccag ctatacccgc atcgctaact
     4141 atcttgaacg ccaggcgcgg caacggggtt atcaactgct aattgcctgc tcagaagatc
     4201 agccagacaa cgaaatgcgc tgtattgagc accttttaca gcgtcaggtc gatgccatta
     4261 ttgtttcgac gtcgttgcct cctgagcatc ctttttatca acgctgggct aacgacccgt
     4321 tcccgattgt cgcgctggac cgcgccctcg atcgtgaaca cttcaccagc gtggttggtg
     4381 ccgatcagga tgatgccgaa atgctggcgg aagagttacg taagtttccc gccgagacgg
     4441 tgctttatct tggcgcgctg ccggagcttt ctgtcagctt cctgcgtgaa caaggtttcc
     4501 gtactgcctg gaaagatgat ccgcgcgaag tgcatttcct gtatgccaac agctatgagc
     4561 gggaggtggc tgcccagtta ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc
     4621 tgttcacaac gtcgtttgcg ttgttgcaag gggtgatgga tgtcacgcta cgtcgcgatg
     4681 gtaaactgcc ttctgacctg gcaattgcca cctttggcga taacgaactg ctcgacttct
     4741 tacagtgccc ggtgctggca gtggctcaac gtcaccgcga tgtcgcagag cgtgtgctgg
     4801 agattgtcct ggcaagcctg gacgaaccgc gtaagccaaa acctggttta acgcgcatta
     4861 aacgtaatct ctatcgccgc ggtgtgctca gccgtagcta agccgcgaac aaaaatacgc
     4921 gccaggtgaa tttccctctg gcg
//
LOCUS       DAZPJP010000255         4928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPJP010000255 DAZPJP010000000
VERSION     DAZPJP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.258"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..539
                     /locus_tag="ACHU9J_004022"
     CDS             <1..539
                     /locus_tag="ACHU9J_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1907439.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPGK
                     DATPGNVYAQADYKIAYE"
     gene            599..3103
                     /locus_tag="ACHU9J_004023"
     CDS             599..3103
                     /locus_tag="ACHU9J_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1907440.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3119..3868
                     /locus_tag="ACHU9J_004024"
     CDS             3119..3868
                     /locus_tag="ACHU9J_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1907441.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3870..>4928
                     /locus_tag="ACHU9J_004025"
     CDS             3870..>4928
                     /locus_tag="ACHU9J_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907442.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1527 a         1010 c         1017 g         1374 t
ORIGIN      
        1 aaaagtcact tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg
       61 aaggtcacgg cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac
      121 caggtgacga agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg
      181 accagaaatc tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg
      241 gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc
      301 agactgacct gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg
      361 tacgtattct ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat
      421 tcaacaatac taacagctat caggaactga acttcaaagc tcgtatggaa tccccgggca
      481 aagatgctac cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat
      541 cacattaggc cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat
      601 gtataaaaaa ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt
      661 atccgttctc atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat
      721 cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc
      781 cgtaggtaaa tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga
      841 cctgcaatgg gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac
      901 attattaggc tttactgatg agattattga agaggctcag caaaacctga tcgatggttg
      961 ttaccctatc gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt
     1021 atccatatct gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga
     1081 actttgggat catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta
     1141 tgcaccacat cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa
     1201 tcttggggcc tggcgcctgc gtactgatta ccagtacgat cagtcattta acaatggcaa
     1261 aagccaggcg aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat
     1321 taatgcaaaa ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca
     1381 tttttctggc gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta
     1441 cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa
     1501 ccgtattatt tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa
     1561 tacattacag gggcaactgg acgtcaaggt tgaagaagag gacgggcaag tcacgcaatg
     1621 gcaagttgca tctaatagta ttccttatct gacgcgtaaa gggcagattc gctacaccac
     1681 tgctatgggt aaaccgacca gcgttggtgg tgattcctta caacaaccct tcttctggac
     1741 tggtgaattc tcatggggtt ggctgaacaa tgtatccctg tatggtggtt cagttttaac
     1801 aaaccgtgat tatcaatctc tggctgccgg cgttggcttt aaccttaact cattgggttc
     1861 attatctttt gatgtcacac gatctgatgc tcagttgcat aatcaggata aagaaacggg
     1921 ttatagctac cgcgctaact attcaaaacg ttttgaatct accggtagcc agctcacttt
     1981 cgctggttac cgtttctctg ataaaaactt tgtgacaatg aatgaatata tcaatgacac
     2041 taaccattac atgaattatc agaatgaaaa agagagttat attgtcacgt ttaaccagta
     2101 tcttgaatca ttaaggttaa atacatacgt aagtttggct cgtaatactt actgggacgc
     2161 cagcagtaat gtgaattatt cattatcact tagccgcgat tttgatatcg ggccattaaa
     2221 aaacgtctcc gcttctctaa catttagccg aataaactgg gaagaagaca accaggatca
     2281 actgtaccta aatatttcga ttccctgggg aaccagtaga acattgagct atggtatgca
     2341 acgaaatcag gataacaaga tttcgcatac tgcttcgtgg tatgactctt ccgatcgaaa
     2401 taattcctgg agcgtttctg cttcaggcga caatgatgaa ttcaaagata tgaaagcgtc
     2461 actacgcgcc agttatcagc ataataccga gaacggtcgt ctctatctct ccgggacatc
     2521 acagcgagac agttattatt ctctgaatgc cagttggaat ggttcattca cggcgactcg
     2581 tcacggagcc gctttccatg actatagtgg tagtgctgac tcgcgtttta tgatcgacgc
     2641 agatggtgca gaagatattc cgttgaacaa taaacgcgcg gtaactaatc ggtatggcat
     2701 cggagttatt ccatcagtca gcagttacat aacaacatca ttaagcgttg atacccgaaa
     2761 tcttccagaa aatgtggata tcgaaaactc ggttatcacc accaccttaa ccgaaggtgc
     2821 tattggctac gccaaacttg atacccgcaa gggctatcaa atcatggggg ttattcgcct
     2881 ggcagatggt agtcacccac cactggggat tagcgtaaaa gataaaacca gccataaaga
     2941 attaggactg gttgctgatg gcggctttgt atacctcaac ggcattcagg atgacagcaa
     3001 acttacttta cgctggggtg ataaatcctg ttttattcaa ccacccaata gcagcaactt
     3061 aaccaccgga acggttattt taccgtgtat tagccaaaat taattcagga tgaaactaat
     3121 gcgctactta aacacaaaga atctcattgc agcaggagta ttactcgcct gcatgagcag
     3181 cattgcctgg ggtgccatca tcccggaccg tacccgtatc ataatgaatg agtcagataa
     3241 aggggaagca ttaaaactta ctaatcaaag caaaaatcta ccctatctgg cgcaaacatg
     3301 gatagaagat acgaaaggca ataaatcacg tgactttatt gtcacggttc cccctatggt
     3361 acgtttaaat cccagtgagc aaattcaaat aagaatgata agtcaggaaa aaattgctca
     3421 actgcctaat gacagagaaa cgctcttcta ttttaacgtg cgagaaatac cgccaaaaac
     3481 agataaaaaa aatgtgatgc aagtcactat gcagcacgca ttgaaactat tctggcgacc
     3541 aaaagctatt gaattagaag atgatggggt aatgtcttac gaaaaagttg aaatcatccg
     3601 tagaaatgat ggttcgatcc gctttaacaa taagatgcct tatcacgtta cgctgggtta
     3661 tatcggaaca aacggaatca caatgttgcc acaaacgcaa agtttaatgg taacccctta
     3721 tagccatgca gatacgcagt ttaaaaatgt tccgtcagct tttcaggttg gttatatcaa
     3781 tgattttggt ggattaagtt tttatgaaat aaactgcccg acggttaata actcttgtaa
     3841 tgtttccgta gccaaacgag ataaataata tgcgctactt attaattgta attatattta
     3901 ttataggttt tagtgcatta cctgtatggg caatggactg ctatgctgaa catgaagggg
     3961 gaaataccgt agtcattggt tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag
     4021 gtgataagat ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat aatgcattac
     4081 ctgccccatc acctggagaa gaatacccat cagcctggac taatataatg atgtttttac
     4141 caggcggtca ggacttttat aatcagaact cctatatttt tggcgtaaca tacaatggag
     4201 ttgattatga ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt gatataaaag
     4261 gagcgggtac ctttaataat cactataaaa accccgctgt ctgtagtgga ggtcctgagc
     4321 ctcaattatc agtaaccttt ccagctcgcg tacaactcta tattaaatta gcaaaaaatg
     4381 ccaatagagt taataaaaac ctggtgttac ctgatgaata tatcgctcta gagtttaaag
     4441 gtatgagtgg cgcaggagct atagaagtag acaaaaattt gaccttcagg attcgtggat
     4501 taaataatat tcatgtcctt gactgctttg ttaatgtcgc actggaacca gctgatggcg
     4561 ttgttgactt tggtaaaata aattcccgaa caattaaaaa taccagcgtt agtgagacgt
     4621 ttagtgtagt catgacaaaa gatcctggtg cggcctgtac tgagcaattc aatattttag
     4681 ggagcttttt cactacggat attttgagtg attatagcca tctggatatg ggtaatggac
     4741 tgctattgaa gatatttcat aaagatggaa cagcaacgga atttaaccgc ttctcacaat
     4801 ttgcttcttt ttcatcgtct agtgcacctt cggtcaccgc accattcagg gcagaactta
     4861 gtgcgaaccc ggcagagact gttgttgagg gaccgtttag taaagacgta atcctcaaaa
     4921 tcacctat
//
LOCUS       DAZPJP010000256         4897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPJP010000256 DAZPJP010000000
VERSION     DAZPJP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.259"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            220..1194
                     /gene="hemB"
                     /locus_tag="ACHU9J_004026"
     CDS             220..1194
                     /gene="hemB"
                     /locus_tag="ACHU9J_004026"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1907443.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1300..2151)
                     /gene="tauD"
                     /locus_tag="ACHU9J_004027"
     CDS             complement(1300..2151)
                     /gene="tauD"
                     /locus_tag="ACHU9J_004027"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1907444.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2148..2985)
                     /gene="tauC"
                     /locus_tag="ACHU9J_004028"
                     /pseudo
     CDS             complement(2148..2985)
                     /gene="tauC"
                     /locus_tag="ACHU9J_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
     gene            complement(2982..3749)
                     /gene="tauB"
                     /locus_tag="ACHU9J_004029"
     CDS             complement(2982..3749)
                     /gene="tauB"
                     /locus_tag="ACHU9J_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1907445.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3762..4724)
                     /gene="tauA"
                     /locus_tag="ACHU9J_004030"
     CDS             complement(3762..4724)
                     /gene="tauA"
                     /locus_tag="ACHU9J_004030"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1907446.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4702..>4897
                     /locus_tag="ACHU9J_004031"
     CDS             4702..>4897
                     /locus_tag="ACHU9J_004031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907447.1"
                     /translation="MLRDEIAMIVCSPVLLWEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWENYYTS"
BASE COUNT         1146 a         1412 c         1243 g         1096 t
ORIGIN      
        1 tagcagcaca accaaacgcc aaaaaaataa taaccaagag ctataaataa agattaaaaa
       61 tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct ggtctctctt
      121 tcattctctt cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc ccgcttcagg
      181 tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt aatccaacgc
      241 cctcgtcgcc tacgcaaatc tcctgcgctg cgcgctatgt ttgaagagac aacacttagc
      301 cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta caaagccgtt
      361 gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga aattgaacgc
      421 atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca taccgatgaa
      481 accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg catctgcaag
      541 cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata cacttctcac
      601 ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct ggaaaattta
      661 ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc ttccgccgcg
      721 atggacggcc aggtacaggc gattcgtcag gcgctggacg ctgcaggctt taaagatacg
      781 gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggcccgtt ccgtgaagct
      841 gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat gaaccgtcgc
      901 gaggcgattc gtgaatcact gctggatgaa gcccagggcg cagactgcct gatggttaaa
      961 cctgctggag cgtacctcga catcgtgcgt gagctgcgtg aacgtactga attgccgatt
     1021 ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct ggcgggtgct
     1081 atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc gggtgcggat
     1141 ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg ttaatttttt
     1201 tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc tcaccctaac
     1261 cctctcccca agggggcgag ggaaccgtcc gcactcgtat caccccgccc gataaaacgg
     1321 tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt aatcggcatt
     1381 ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt gccagcgcca
     1441 acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca aggcttcgct
     1501 ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa acagcgcctg
     1561 tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat ttttcgcgac
     1621 cgcctcgcgc cagcgttgat gttcctcctc ggttttgcgg tatttgtatt ccgggaacga
     1681 tttacggaaa tcatgctccg cacgcagccc gctcagcagc tggcggaagg gaacagagag
     1741 cgcctcatag gccgcaatac cgctggtcca gagcgtatca ccgccggtcg aaggtaactc
     1801 tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat cggtatgcca
     1861 gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct cgtcaacccc
     1921 ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg ccagcgcgcg
     1981 ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatagc gcagcaccgc
     2041 atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg cacccgaaat
     2101 ttgtgcgccg atatacggcc ccagcggggt aatactcaga cgttcactca ttgtacttct
     2161 ccatgccagg gcgtcagacg gcgctgtaac gcgcgcagac ccagttctaa aagaaaggcg
     2221 ataatcgcaa tcaccgcgat ccccgccagt accacgtcag tcgcgagaaa ttcacccgct
     2281 gactgaacca taaatcctaa accgcgtgtc gcggcaatca gctccgccgc taccagcgta
     2341 gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag cgcgccgggc
     2401 aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc ggcacgaatg
     2461 cgaacctgct gcgcgctttt cacccccgcc agcaccgaca tcgccaccgg tgcaaaaatc
     2521 gctaaataga tcaataagat cttcgaggtt tcaccaatac caaaccagat caccatcagc
     2581 ggcaaataag ccagcggcgg caccggacga taaagttcga ttaccggatc cagaatgccg
     2641 cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac caccgccgcg
     2701 agtagcgcca gcacaatgcg cgtcaggctg gctgccagat gctgccagat gctgccacag
     2761 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     2821 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     2881 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     2941 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     3001 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3061 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3121 agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa caccgcttct
     3181 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3241 agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc gtcgagtaat
     3301 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccaccg
     3361 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     3421 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     3481 ttgtcctgta cattgcgcca cggtagtagc ccttcattct gaaaaaccac cccgcgctct
     3541 gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     3601 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     3661 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     3721 tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     3781 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     3841 tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     3901 ggcgtcagat aggtattccc cttcaccagc cccggaacgt caccttccgg cacgccgctt
     3961 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtctgg gttagcaatg
     4021 tacggttgct gagcatcgat ggcgctttta gcgaacgctt tcacgacctc aggatgtttc
     4081 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4141 tgttcagaat cggtcaacac cttgccgtct ttttccaggg cgttaaccgc cggtgcccag
     4201 acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg cggctgcagg
     4261 ttcacaatct ccacttgccc tggtttaatc ccccagtgtt tcagcgccgc cagcaggctg
     4321 tagtgggtgg tggagataaa cggtacggca atgcgtttgc caatcaaatc ttcgggtttg
     4381 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaag
     4441 acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggaacc gaggttgccg
     4501 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     4561 cagtccacgc tcgccccgct ctctttagca aaggtgttgt cggcctgagc cactttcgcc
     4621 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     4681 aatgccagtg cggcaagaag tgtgttacgc gatgaaattg ccatgattgt ctgctcccct
     4741 gtcttgttat gggagcagta ttcaggaata aaaacattca ttaaaagaat tagtcgttat
     4801 cgcacagatg attttattct tagcaaaaaa acggcaaagg aaagattaaa tatgcaatta
     4861 ctccgttcta acaattggga aaattactac acttcat
//
LOCUS       DAZPJP010000257         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPJP010000257 DAZPJP010000000
VERSION     DAZPJP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.260"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(111..2180)
                     /gene="glyS"
                     /locus_tag="ACHU9J_004032"
     CDS             complement(111..2180)
                     /gene="glyS"
                     /locus_tag="ACHU9J_004032"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1907448.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2190..3101)
                     /gene="glyQ"
                     /locus_tag="ACHU9J_004033"
     CDS             complement(2190..3101)
                     /gene="glyQ"
                     /locus_tag="ACHU9J_004033"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1907449.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3196..3495)
                     /locus_tag="ACHU9J_004034"
     CDS             complement(3196..3495)
                     /locus_tag="ACHU9J_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1907450.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3670..4665
                     /gene="wecH"
                     /locus_tag="ACHU9J_004035"
     CDS             3670..4665
                     /gene="wecH"
                     /locus_tag="ACHU9J_004035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1907451.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4707..>4870)
                     /locus_tag="ACHU9J_004036"
     CDS             complement(4707..>4870)
                     /locus_tag="ACHU9J_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1907452.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1120 a         1270 c         1230 g         1250 t
ORIGIN      
        1 ttaatatatg agataatctt tattcaacag aagattatta aaggttgctg tattatttag
       61 cgataataaa aaaagcctgc cagatggcag gctttttaat aacggcgtta ttactgcaac
      121 agcgaaatat ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat ggtcagacgg
      181 ttgagacgca attctttgtc atcaaccatg accatcactt tatcgaagaa agcatcaacc
      241 ggctcacgca gctctgccag ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac
      301 ggctccagct tgtcacgtag caccacaacc tgcatcgcca gtttaatttc ttccggctct
      361 ttcagggtag aggcattcac gcggtcgctc agcacttcgt cagatttcgc cagaatgtta
      421 gatacacgtt tgttcgccgc cgccagtgca gcagctgcat ccagggtacg gaaatgcgat
      481 accgctttca tacgggcatc gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc
      541 tggatggtgt caacggtgta accttcgtcc tgataccagg cgcggaagcg accgagcata
      601 aagtcgataa catcatcaac tacgttggcg ttagtcagct tatcgccata cagacgcact
      661 gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct tctcaacgat aattcgcagc
      721 acgccaagtg cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg atgctgaccg
      781 ataccgaaga tacccgccag agtatccatc ttatcagcaa tcgccagcgc acaagctacc
      841 gggttggacg gcaggtcatc accggcaaag cgcggctgat actgctcatt cagcgcgacg
      901 gctacgtctt ccgcttcgcc atcgtgacgc gcgtagtgca tccccataac gccttgggtg
      961 tcggtgaact cgaagaccat gttggtcatc aggtcacact tagacagcag gcccgcacgg
     1021 gttgcgtggt taacgtcagc gccaatctgt tcagcaatcc agccagccag cgcctggatg
     1081 cggtcagttt tgtcacgcag cgtacccagc tgttgctgga acagcacggt ttgcaggcgc
     1141 ggcaggttat cttccagacg ttttttacgg tcggtattga agaagaactc ggcatccgcc
     1201 agacgtggac gaacgacttt ctcgttacca gagataattt gctgcggatc tttcgattcg
     1261 atgttggcaa cgaagatgaa gttcggcagc agtttgccat cgttcgcata caccgggaag
     1321 tatttctggt cacctttcat ggtgtaaacc agggcttcag ccggcaccgc gaggaatttc
     1381 tcttcgaatt ttgcggtcag aacgaccggc cactccacca gcgaagccac ttcttccagc
     1441 aggctttcgc ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc ggcatcggct
     1501 ttaatcttcg ctttacgttc ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt
     1561 tccggatact gatcggcgtt gtcgatggtg aattccggtt cgcccataaa gcggtggccg
     1621 cgaatcacgc gatcggactg aatgcccaga atggttgccg gaatgacttt gtcgcccagc
     1681 agcagggtca cggtgtgcac cggacgcacg aagtgcacgt cgcttgcgcc ccaacgcatc
     1741 agtttcggga tcggcagttt cgccagcgaa gtcgcaacca tattcggcag tagtgcttca
     1801 gtgctttcgc ccttcacatg ggcgcgatac agcagccatt cgcctttatc ggtagtcaga
     1861 cgctcagcct ggtcaacggt aataccgcaa ccacgcgccc aaccttctgc cgctttgctc
     1921 ggtttgcctt cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc gatttcacga
     1981 tctggttgcg cttccgccag gttagctact ttcaacgcca gacgacgcgg agcagcaaac
     2041 cattgaacgg tgccgtgtgc gaggccagcg ttatccagct ccgcagtaaa gttcgcagca
     2101 aaggactcag ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc gatttccacc
     2161 agaaaagttt tctcagacat agccgcctct tacttatctt tgttgcacat cgggaagccg
     2221 agggcttcac gggaagcgta gtatgcttct gccactgctt tggtcagggt gcgaatgcgc
     2281 agaatgtagc gctgacgctc agtgacggag atggctttac gcgcatccag caggttgaag
     2341 ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg gcagcggatt ttccagcgcc
     2401 agcagctgct gcgcttcttt ctcgtactgc tcgaagcagg tgaacaggaa atccacatcc
     2461 gcgtattcga agttgtaagt ggactgctcc acttcgttct gatggaacac gtcgccgtag
     2521 gtggttttac ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc tacgccctga
     2581 atgtacatgg ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg tttacactcc
     2641 agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt tagccacact
     2701 tcccagccca gtccccaggc acccagagtc gggttttccc agttatcttc cacgaaacgg
     2761 atgtcgtgaa tagtcgggtc catgcccagc tctttcagag aaccgaggta cagctcctga
     2821 atattgtccg gcgatggctt aatgaccacc tggaactgat agtagtgctg taaacggttg
     2881 gggttttcgc cgtagcgacc atcggtcggg cgacgagaag gctgaacata agcagccgcc
     2941 atcggttctg gccccagcgc gcgcagacag gtcattgggt gagaggttcc cgcgccgact
     3001 tccatgtcca atggttgaac aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc
     3061 aggatcaagc cctggaaggt cctggtatca aacttttgca tattatttcg tgctggatac
     3121 gtgtggattt aaaggaaggg atcagtatac ccgctggatt gaagatatac agtacgaaac
     3181 gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga
     3241 acagacaaac tgctctttac ggaaggtata accgcgcatt tcatagcttc cctggaactg
     3301 ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta taacgttttg ccgcctgatc
     3361 cttgcataac gcttccatat tcaacgaacg cagaggatca acctttaccc gttgtgcttt
     3421 ctgcacgggt gacggtgtag accaacccac tagcaccata agcgccattg ccggaaagaa
     3481 agcgttcatc atcattaaat taccgtctgg tttgcaggtg agtcttatta tttatatcgg
     3541 tatagtggct gatatcttac gaatttcctt aagcctctat aagctgtagt gataaaactc
     3601 agattattcc agtgcgccaa ccaaatgatt tatcggtcag acttttttta accagataca
     3661 gagacacaga tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta
     3721 atggtggtga tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc
     3781 gtcacatggg atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt
     3841 ttcatgattt ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg
     3901 cgtatcggct tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt
     3961 acctccatca atatggagtt agcgctgaaa aacctgctgc aaaagccagt gttttaccac
     4021 ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag
     4081 aacgtcggcg gaaaaatgtt gctggtgcta atggcggtga ttggcattat cgctaaccca
     4141 aacacagtgc cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat
     4201 ggcgatactt tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac
     4261 acacagcata aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt
     4321 atctctcgcg ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat
     4381 ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac
     4441 acgctggata cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata
     4501 tacggattcc acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat
     4561 tggccaatac tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt
     4621 tctatgctgg tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca
     4681 cattggactg accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt
     4741 tcttttggcg ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac
     4801 aagcttaaga agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc
     4861 cacagaccta
//
LOCUS       DAZPJP010000258         4860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPJP010000258 DAZPJP010000000
VERSION     DAZPJP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.261"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHU9J_004037"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHU9J_004037"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1907453.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHU9J_004038"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHU9J_004038"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1907454.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1089 a         1357 c         1249 g         1165 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa cagaagacaa
//
LOCUS       DAZPJP010000259         4834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPJP010000259 DAZPJP010000000
VERSION     DAZPJP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.262"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHU9J_004039"
     CDS             complement(108..551)
                     /locus_tag="ACHU9J_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1907455.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHU9J_004040"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHU9J_004040"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1907456.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHU9J_004041"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHU9J_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHU9J_004042"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHU9J_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1206 a         1273 c         1225 g         1130 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattccaga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aacc
//
LOCUS       DAZPJP010000260         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPJP010000260 DAZPJP010000000
VERSION     DAZPJP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.263"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHU9J_004043"
     tRNA            6..81
                     /locus_tag="ACHU9J_004043"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHU9J_004044"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHU9J_004044"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHU9J_004045"
     CDS             complement(299..1057)
                     /locus_tag="ACHU9J_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1907457.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHU9J_004046"
     CDS             complement(1065..2168)
                     /locus_tag="ACHU9J_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1907458.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHU9J_004047"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHU9J_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHU9J_004048"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHU9J_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPJP010000261         4766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPJP010000261 DAZPJP010000000
VERSION     DAZPJP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.264"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            21..542
                     /locus_tag="ACHU9J_004049"
     CDS             21..542
                     /locus_tag="ACHU9J_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR1907459.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQTQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            598..831
                     /locus_tag="ACHU9J_004050"
     CDS             598..831
                     /locus_tag="ACHU9J_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR1907460.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGAHFRLV
                     VRNNSGLVWRTWNFEEGAGYWMNHVIRNFGILK"
     gene            892..2850
                     /locus_tag="ACHU9J_004051"
     CDS             892..2850
                     /locus_tag="ACHU9J_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR1907461.1"
                     /translation="MSVAKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERGILTADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGYLKKPQIIAALNDAGLSGAARDAERMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPRPQTDTTERTDNLADAA"
     gene            2905..3339
                     /gene="psiB"
                     /locus_tag="ACHU9J_004052"
     CDS             2905..3339
                     /gene="psiB"
                     /locus_tag="ACHU9J_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR1907462.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFYLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3336..4055
                     /locus_tag="ACHU9J_004053"
     CDS             3336..4055
                     /locus_tag="ACHU9J_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR1907463.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLTGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4272..4424
                     /locus_tag="ACHU9J_004054"
                     /pseudo
     CDS             4272..4424
                     /locus_tag="ACHU9J_004054"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            4369..4491
                     /locus_tag="ACHU9J_004055"
     CDS             4369..4491
                     /locus_tag="ACHU9J_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1907464.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1079 a         1236 c         1504 g          947 t
ORIGIN      
        1 tcaacagaag gagatacatc atggcagttc gtggcattaa caaggtcatt cttgtcggtc
       61 gtctgggaaa agatccggaa gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc
      121 aggtggccac gtcagaaagc tggcgtgaca aacagacggg ggagatgcgg gagcagacag
      181 aatggcatcg cgtggtgctg ttcggcaagc tcgcggaagt ggcaggtgaa tatctgcgca
      241 agggcgcgca ggtctacatc gaggggcaac tccgaacccg tagctgggaa gataacggta
      301 ttacccgtta cgtcactgaa atccttgtga aaaccacggg caccatgcag atgctgggga
      361 gtgcaccaca gcagaacgct cagacgcaac cggagcctca gcagaatggt cagccacaga
      421 gtgctgacgc gacgaaaaaa ggtggcgcga aaacgaaagg ccgtggacgt aaggccgcgc
      481 agcccgagcc tcagcagccg tctgaacccg cgtatgactt cgatgacgac attccgttct
      541 aacgaaagac agtgacaacc gccccgtcca gtgcggggca tcaccggaga gatgaggatg
      601 agcgaatatt tcagaatatt tcaggggctg cctgacggct cctttacccg cgaacaggct
      661 gaagccgttg ccgcacagta ccggaatgtc tttatcgagg atgatcaggg agcgcatttt
      721 cgcctggttg tccgcaataa cagtggcctg gtctggcgta cctggaattt tgaggaaggt
      781 gccgggtact ggatgaacca tgtcatccgg aatttcggga tccttaagta agagaggtgc
      841 cggacgggga tcacgtccgg cagacattaa ctacacaata aggggatagt catgtcagta
      901 gcaaagtctg aaccaaaatc tacccgtaaa gcaagccgta aacctgcaaa aacgcaggaa
      961 actgtcctgt ctgccctgct ggcgcagacg gaggaagtga gcgtgccgct ggattcactg
     1021 attaaatcac cgctgaatgt gcgtacggtg ccgtattctg cggagtctgt cagcgaactg
     1081 gcggactcca ttaaaggtgt cggcctgtta cagaatctgg ttgttcatgc cctgcctggt
     1141 gaccgttacg gtgtcgccgc aggtggtcgc cgactggctg cactcaacat gctggcagag
     1201 cgtggcatcc ttacggctga ctggcctgta cgcgtgaagg tcattccgca ggagctggcg
     1261 actgccgcat cgatgaccga gaacggtcag cgtcgggata tgcaccctgc cgaacagatt
     1321 gccggattcc gcgcaatggc gcaggaaggc aaaacacctg cacaaatcgg tgatttgctg
     1381 ggctattcac cccgccacgt tcagcgaatg ctgaaactgg ctgaccttgc gcctgtcatc
     1441 ctcgatgcgc tggcagaaga ccgcatcacc accgagcact gtcaggcgct ggcgctggag
     1501 agcgacaccg cgcgtcaggt gcaggtgttt gaagctgcct gtcagtcggg atggggcgga
     1561 aaaccggatg tgcgggttat ccgcaatctg attacggaaa gtgaagtggc ggtggcgggg
     1621 aacagtaaat tccgcttcgt gggggctgat gccttctcgc cagacgaact gcgcaccgat
     1681 ttgttcagcg acgacgaggg tggctatgtg gactgcgtgg cgctcgatgc cgccctgctg
     1741 gaaaaactcc aggctgtcgc agagttcctt cgggaagccg aaggctggga atggtgcgcc
     1801 ggacgcatgg agcctgtcgg tgagtgccgt gaggatgccg gaacataccg ctgtctgccg
     1861 gagccggaag cggtgctgac cgaggcggaa gaagaacgtc tgaacgaact gatggcacgt
     1921 tacgacgcgc tggaaaacca gtgtgaggaa tctgacctgc tggaagcaga aatgaagctg
     1981 atgcgctgca tggcgaaggt cagagcgtgg acgccggaga tgcgtgccgg aggtggagtg
     2041 gtggtgtcct ggcgttatgg aaacgtatgt gtccagcgtg gtgtgcagtt acgcagtgag
     2101 gatgatgcgg ctgacgacgc tgaccgcacg gaacaggtgc aggaaaaagc gtcagtggag
     2161 gaaatcagtc tgccactgct gacgaaaatg tcttccgaac gcacgctggc agtccaggca
     2221 gcactgatgc agcaaccgga caaatccctg acactgctgg catggacgct ctgcctgaat
     2281 gtgtttggca gcggagcgta cagtaaacca gcacaaatca gcctggaatg taaacattat
     2341 tcgctgacca gcgatgcgcc atcggggaag gaaggtgccg cattcatggc gctgatggca
     2401 gaaaaagccc gtcttgctgc cctgttaccg gagggatggt cacgggacat gacgacattc
     2461 ctgtcactca gtcaggaggt gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt
     2521 aacggtgtcc agacccgtga gtgtggtcac acgtcacgca gtccgcttga cacgctggaa
     2581 accgctatcg gattccacat gcgcgactgg tggcagccga caaaagcaaa cttcttcgga
     2641 tacctgaaaa agccgcagat tatcgcagct ctgaatgatg ccggactgtc cggtgccgca
     2701 cgggatgcag agaggatgaa gaaaggtgat gcggctgaac atgcagagca ccatatgaaa
     2761 gacaaccgct gggtgcctgg ctggatgtgt gcaccacgtc cacagacgga taccactgaa
     2821 cgcaccgata acctggctga tgccgcctga tgaacaacca caccgccccg ccggagacgg
     2881 ggcggcagca agggagatac cgtgatgaaa actgaactga ccctgaatgt attacagacc
     2941 atgagtgcac aggaatatga agatatccgg gctgccggaa gtgatgaacg ccgtgagctg
     3001 acacacgccg tgatgcggga actggatgcg ccggacaact ggacgatgaa tggcgagtac
     3061 ggcagcgagt tcggcggctt tttccccgtc caggtccgtt tcacgcctgc ccacgaacgt
     3121 ttttacctgg cgttatgttc accgggcgat gtctctcagg tctgggtgct ggttctggtg
     3181 aatgccggtg gtgagccgtt tgcggtggtt caggtgcagc ggcggtttgt accggaagcc
     3241 gtcagccatt cactggcact ggcggcgtca ctggatgcgc aggggtacag cgtcagtgac
     3301 atcatccata tcctgatggc ggaaggaggt caggcatgag cgtacgttca caggcactgg
     3361 ttcccctcag cgcagagcaa caggccgcat ggcgggcggt ggcggagacg gaaaaacgcc
     3421 gccatcaggg taacacgctg gcggaatatc cgtatgcggg ggcgtttttc cgctgtctga
     3481 acggaagccg caggatatcg ctgtctgacc tgcgattttt catgccgtcc ctgaccgcag
     3541 aagaactgca cggcaaccgt cttcagtggc tgtatgccat cgatgtgctg attgaaacac
     3601 agggcgaggt ctgcctcctt ccccttccag gcgacgcagc agaacgcttg ttcccgtcgg
     3661 tgcggtttcg tgtcagggaa cgcagtcgcc ataaatccgc gctggtcatg cagaagtaca
     3721 gccgccagca ggcgagggag gcagagcaga aagcccgtgc gtaccaggcg ctggtcgctc
     3781 aggctgaaat cgaactggca ttccattcgc cggaaacagt cggaagctgg catgcacgct
     3841 ggagtgaccg ggtggcagag catgacctgg aaaccctgtt ctggcagtgg ggcgagcgtt
     3901 ttccgtcact gaccgggatg gaacgctggc agtggcagga tatgccattc tggcaggtca
     3961 tcgcagaggc tggtatggcg gcaagggagg ccggtcatgc cgtccgtgag atggagcgat
     4021 ggatggtgcc gaacaaactc cgggaggcgg cgtgatgcgg caacaatcac acagattacc
     4081 cgtaaacagc ctgaatgagc gggttatttt cagggaaagt gagtgtggtc agtgcgcagg
     4141 tatatgggct atgatgtgcc cggcgcttga ggctttctgc ctcatgacgt gaaggtggtt
     4201 tgttaccgtg tcgtgtggca gaaggacaaa agccccgtag ttaatttttc attaacccac
     4261 gaggcctctg catgtctagt ccacgtcagt atggcctctt accgtgcaaa ttgcaaggag
     4321 gagtaaacca tgaaactacc gggaaacgcc cttatctggt gcgtattaat cgtgtgctgc
     4381 acgttgttga tattcacact cctgacccgg aatcgcctgt gcgaagtccg gctgaaggac
     4441 ggatacaggg aagttacggc aacaatggct tacgaatccg gcggtaagta gcaacctgga
     4501 ggcgggcgca ggcccgcctt ttcagggatg atgctggtct gactgcactg aagcgccttt
     4561 ataaaggggc tgctggttcg ccggtggccc ctttctcctt gctggcattg tgcgggcatg
     4621 aacaaccaga ctgccaggaa ggccgtttcc ctccgggaac ggggaagaac gcgcgcctgc
     4681 ggcgcccgtt cttttccccg ccctctctgt ttatggccag ttgtcagaaa tgacattacc
     4741 tttcccgtga ttattctctt tttctt
//
LOCUS       DAZPJP010000262         4715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPJP010000262 DAZPJP010000000
VERSION     DAZPJP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.265"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..738)
                     /locus_tag="ACHU9J_004056"
     CDS             complement(<1..738)
                     /locus_tag="ACHU9J_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1907465.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHU9J_004057"
     CDS             complement(879..2912)
                     /gene="metG"
                     /locus_tag="ACHU9J_004057"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1907466.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3044..4153
                     /gene="apbC"
                     /locus_tag="ACHU9J_004058"
     CDS             3044..4153
                     /gene="apbC"
                     /locus_tag="ACHU9J_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1907467.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4398..4679
                     /locus_tag="ACHU9J_004059"
     CDS             4398..4679
                     /locus_tag="ACHU9J_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1907468.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1135 a         1173 c         1185 g         1222 t
ORIGIN      
        1 aatatcaatc tgttgaaggt gtattattac atcagtagca tattccagac cgccttcctg
       61 gacgatctga tccataacat cctcacgcac tttatatctt gtgcatttca tttcaagtgc
      121 cgtgagtaca gccattactg gtgatggtaa ctgtgttgtt ttcaggccgt cgatctgact
      181 gatcgcctgg gcgacggtgt tttgccattc taaagaacag gcgctgaagt caaatgtggc
      241 tgttttatta tcccgttgcg acacactaca ggctaagcga cgtaattggg tcaatttgtc
      301 tgcttgctga acaggaactc cgggaaagcg gcgatgactt aacgtggtgt gtacaacttc
      361 gggcggaaaa acgacagcaa tatctttcgc cagccacggt aaaacagcat ctgtattttt
      421 gtcaggcgca cgggtttctg ctacgaaaga gggttgttcc tgaataatgg tggataagtc
      481 cgctaccttg agagagcccg ttaccgtttg ggaaggttgc aaagaattat ccttcgcttg
      541 ctccacatac cccttcttca ccttctccgc aatcagctta agctccgctt ttgccgctgc
      601 cgcagcatct gaaaaacttt ttatctggct ttgccctttg gtgccaactt ttccccaact
      661 gatatgcaac tcgtttccct gttgctcaac tgcccagaat ttatgtgatt tttcgtcctg
      721 atagataaag tgtctcattc tgcatccctg taattttatc cagttgaatg cagatgctac
      781 cagtatttat gcgggttaga gagagacaaa atcgcaagaa aacaacttta ggaatttata
      841 aagaaaaggc gctgtcgatg cagcgccttg aagggggatt atttcacctg atggcctggt
      901 ttagcaccag catccgggct taacaggaag atatctttcc cgccaggacc agcagccatt
      961 accatgcctt cggagatacc aaagcgcatt ttacgcggtg ccaggttggc caccataatg
     1021 gtgtgacgcc cgatcagtgc ctgcggatcc gggtaggcag aacgaatacc ggagaagaca
     1081 ttgcgttttt caccgccgag atccagtgtc aggcgcagca gtttgtcaga accttcaaca
     1141 aactctgcgt tttcgatcag cgccacgcgc aggtcaactt ttgcgaagtc gtcaaaggtg
     1201 atggtttcct gaatcggatc atctgccagt gggccagtaa ctggcgcggc agctgctttc
     1261 acttcttctt tagacgcttc caccagtgct tcaacctgct tcatatcgat gcggttatac
     1321 agcgctttga acggattcac tttgtggccc agcagcggtt gctggatacc atcccaggtc
     1381 agttctgtat tgaggaatgc ttctgcgcgc tcggtcagtt tcggcagtac cggcttcagg
     1441 taagtcatca gcacgcggaa caggtttatg cccattgagc aaattgcctg caggtcggca
     1501 tcgcggcctt cctgttttgc caccacccac ggagcctgct cgtcgacata gcggttagcc
     1561 agatcagcca gcgccatgat ttcgcgcact gctttaccaa attcgcggct ttcccacgct
     1621 tcaccaatca cttcagcggc atcagtgaag gttttgtaca actgcgggtc agccagttcg
     1681 cttgccagca cgccgtcaaa acgcttgttg ataaagcccg cattacgaga ggccaggtta
     1741 accactttgt tcacgatatc ggcattcaca cgctgaacga aatcctccag gttgagatcg
     1801 atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt aacgcaggct gtcagcgtca
     1861 aaatgattca gccaggtgct ggctttaata aaggtgccgc gagacttgga catctttgcg
     1921 ccgttcaccg tcacatagcc atgaacaaac aggttggtcg gcttgcggaa gttgctgcct
     1981 tccagcatgg caggccagaa caggctgtgg aagtaaacaa tatctttacc gatgaagtgg
     2041 tacagctcgg cggtggagtc tttcttccag tattcatcga agcttacgct gtcgctgcgc
     2101 ttgtcgcaca gattcttgaa agaacccatg tagccaatcg gtgcgtccag ccagacgtag
     2161 aaatatttgc ccggcgcgtt cggaatttca aaaccaaagt atggcgcgtc gcgggagata
     2221 tcccactgtt gcaggccgga ttcaaaccac tcctgcattt tatttgccac ctgctcctgc
     2281 aacgcaccgc tgcgggtcca tgcctgcaac atttcgctga aagagggcag atcaaagaag
     2341 aagtgttcag aatcacgcat taccggcgta gcgccagaaa ccaccgattt cggctcgatc
     2401 aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt tatcgccgta ttgatccggg
     2461 gatttacatt tcgggcaggt gcctttcaca aaacggtccg gcaggaacat gcctttttcc
     2521 ggatcgtaca gctgagagat ggtgcggttt ttaataaaac cgttctcttt caggcgagag
     2581 tagataagtt ctgacaactg gcggttctct tcgctgtgcg tcgagtgata gttgtcatag
     2641 ctgatgttaa agcctgcgaa atcagtctga tgctcctgac tcatttcgcc aatcatctgc
     2701 tccggggtga taccaagctg ctgagctttc agcatgatcg gtgtaccgtg ggcatcgtcg
     2761 gcgcagatga agttgacctc gtggccgcgc attcgctggt aacggaccca gacatcagcc
     2821 tggatgtgct ccagcatatg gccgaggtgg attgagccgt tagcgtacgg cagtgcgcac
     2881 gtcaccagaa ttttcttcgc gacttgagtc atagtaggca ttacttctta atagtgaaaa
     2941 ggggttagat agtaccaaat gggaaaatgt taagtaagta cttctctata ttcttcaggt
     3001 aaactatcag ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca
     3061 ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca
     3121 cccaaccctg aagcataacc tcaccacgct gaacgcgttg caccatgtcg cctggatgga
     3181 cgacacgttg catgtggaac tggttatgcc attcgtctgg catagcgcgt ttgaagagtt
     3241 aaaagagcaa tgtagcgccg aactgctgcg tatcaccggg gcgaaagcta tcgactggaa
     3301 actgtcgcac aatatcgcca ccttaaaacg cgtgaagaat cagccgggta ttaatggtgt
     3361 gaagaacatc attgcggtca gttccggtaa aggcggcgtg ggtaaatcct ccacggcggt
     3421 aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc ggcattctgg acgccgatat
     3481 ctacggccca tcaattccca ccatgctggg cgcagaaaat cagcgtccaa cttcgccgga
     3541 cggtacccat atggcaccga ttatgtctca tggcctggca accaattcaa ttggttatct
     3601 ggtcactgac gacaatgcga tggtgtggcg tggaccgatg gcgagcaagg cgttaatgca
     3661 gatgttgcag gaaaccttgt ggccggatct cgactatctg gtgcttgata tgccgccagg
     3721 caccggtgac attcaattga cgctggcgca aaacattcca gtaactggtg cagtcgtggt
     3781 caccaccccg caggacatcg ctttgatcga tgcgaaaaaa ggcattgtga tgttcgagaa
     3841 agtcgaggtt ccggtgcttg gtattgtcga gaacatgagt gtgcatattt gcagtaactg
     3901 tggtcaccat gaaccgattt tcggtaccgg tggtgcagag aaactggctg aaaaatacca
     3961 cacacagctg ctggggcaga tgccgttaca catctcctta cgtgaggatc tcgataaagg
     4021 cacgccaacg gttatcagcc gtccggagag cgaatttacc gctatctacc gccagctggc
     4081 tgaccgcgtt gcagctcagc tctactggca gggtgaagtc attccaggcg agatttcctt
     4141 ccgcgcggtc taacgcctat taaccattct ggttattctt catacaccgc agagatgtta
     4201 cattgatgcg gtgtattagt ttttgccgca ttaatgaaca cttatttaat actgcgaata
     4261 ttctgcggac cttcgtccga aactctacaa agtcgattga tcatacaggc aatgcttcat
     4321 tatcatcctt cgtggctgtg tagtggatgt acagctatag tttgttatgc actcattcta
     4381 tccaggcata accttcaatg aataagtatt ggctgtcagg aattattttt cttgcctatg
     4441 gattggcttc tcccgcattc tcttcagaga ctgccacatt agcgattaat ggcaggatca
     4501 gtcccccaac atgcagtatg gctatggtta atggtcaacc tcaaccgcat tgtggtcagt
     4561 taacgtataa tgttgacacc cgtcatctgt tttcttcgcc cgtcaaaggt gtaacgacag
     4621 aagtggttgt tgcaggtagc gatagcaaac gtcgaatcgt gttaaatcgc tacgattaat
     4681 ttggcaacct gattttaatt gcatttagaa agccg
//
LOCUS       DAZPJP010000263         4650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPJP010000263 DAZPJP010000000
VERSION     DAZPJP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.266"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(19..2115)
                     /locus_tag="ACHU9J_004060"
     CDS             complement(19..2115)
                     /locus_tag="ACHU9J_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1907469.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(2115..2861)
                     /locus_tag="ACHU9J_004061"
     CDS             complement(2115..2861)
                     /locus_tag="ACHU9J_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1907470.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(2858..3502)
                     /gene="gfcB"
                     /locus_tag="ACHU9J_004062"
     CDS             complement(2858..3502)
                     /gene="gfcB"
                     /locus_tag="ACHU9J_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1907471.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(3609..3914)
                     /locus_tag="ACHU9J_004063"
     CDS             complement(3609..3914)
                     /locus_tag="ACHU9J_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907472.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTH"
     gene            complement(4361..4573)
                     /gene="cspH"
                     /locus_tag="ACHU9J_004064"
     CDS             complement(4361..4573)
                     /gene="cspH"
                     /locus_tag="ACHU9J_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1907473.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         1185 a         1148 c         1143 g         1174 t
ORIGIN      
        1 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
       61 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
      121 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa
      181 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
      241 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cccctttatc
      301 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
      361 ccagtaagcc gtcacgaaac cagttggcgt ggaataatcg gtgaagcgca tcatgttgtc
      421 ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
      481 gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac cgccatacac
      541 ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt tgttgagata
      601 aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
      661 gggcaactgc gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgcc
      721 atcaagcagc aggtggtcgg taaaccagta gcgggcactg gacatcagcc ccagctggaa
      781 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
      841 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt ctgcttcaac
      901 acgttgttgt tgaagttgct ctgattgacc aggcgctgta ccagccagct gtttgcgcag
      961 gctggctacg tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct
     1021 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
     1081 ttcgcgagaa tcacggtatt tgtattgttg accagacata tacagcgtct tatcgcgcag
     1141 ctgaatttcc ggtgcttcaa aacctgcgtt gtacttcagc gcggtaagtt ggttcgctac
     1201 tgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
     1261 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt
     1321 gttaccgcgt tcataactca ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc
     1381 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
     1441 atcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
     1501 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
     1561 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
     1621 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
     1681 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgccac
     1741 ttgcggtagc cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt
     1801 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
     1861 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta
     1921 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
     1981 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
     2041 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
     2101 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
     2161 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
     2221 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
     2281 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
     2341 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccggcccc agaaaccgca
     2401 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac
     2461 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
     2521 ccagtgatat tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg
     2581 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagt
     2641 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
     2701 agttgcggtt gtgtcaccaa ctggacaaca ttttccatgg ggccaacgga aagtgtctgt
     2761 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
     2821 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
     2881 tcatttctac gggaataacc cctgcgccta acatttgccg actttgacga acctggccag
     2941 tagccgaatc gacccagaat gtgttttgcc actgtttttc tggccgcgtg gaagtcacct
     3001 cttcctgcca gacattacac agcacattgc gacccgcaat attcagcgtc tctaatccag
     3061 caaaggaaaa agtagaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
     3121 tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc gttacgcaga gggtcctgcc
     3181 cggaatttgt cacttccagc agattattat taagtttgac ggtttttaaa agacgtccat
     3241 tgtgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
     3301 atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag
     3361 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
     3421 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
     3481 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
     3541 tctaaagccc ggtacgaata tcgcaaccgg gtatcattta actttaattt cgacgcgtta
     3601 aagcagtatt agtgtgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
     3661 ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
     3721 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagtagc agtcgtggtt
     3781 gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt
     3841 gcttcagagg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
     3901 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
     3961 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
     4021 actatatact gatactgcgg gtaggaaagg tgcgcagagg gaaatataag attattaacg
     4081 atcccctctg gaaatacgac ttgtgagcat aagataaatc ttagcaaaat acaaattaaa
     4141 gataaggaaa ttgttacgaa agctattaat tattgttggt aatatagttt caagtggtac
     4201 tataactggc tgttaaatgg catttataac tattaggtgc agagatattc gcttaaatgg
     4261 attagtttct ttgtggaaaa gtaactgatg ttataatgta tgatgttgac tattgcacta
     4321 ttccaataaa accagtcagc tttaaacaag cagcgtcata ttaagagaga taaacatttg
     4381 ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat cctggtataa
     4441 gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct ttacgaccgt
     4501 cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca attcctgtca
     4561 ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac ggggttaata
     4621 aaataaagcc agcgatattt aagaccgccg
//
LOCUS       DAZPJP010000264         4612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPJP010000264 DAZPJP010000000
VERSION     DAZPJP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.267"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHU9J_004065"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHU9J_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHU9J_004066"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHU9J_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1907474.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1168 a         1193 c         1122 g         1129 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ct
//
LOCUS       DAZPJP010000265         4593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPJP010000265 DAZPJP010000000
VERSION     DAZPJP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.268"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1380)
                     /locus_tag="ACHU9J_004067"
     CDS             complement(<1..1380)
                     /locus_tag="ACHU9J_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1907475.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNN"
     gene            complement(1437..3809)
                     /locus_tag="ACHU9J_004068"
     CDS             complement(1437..3809)
                     /locus_tag="ACHU9J_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1907476.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(3847..>4593)
                     /locus_tag="ACHU9J_004069"
     CDS             complement(3847..>4593)
                     /locus_tag="ACHU9J_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1907477.1"
                     /translation="SRQAFMLVSNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPT
                     NKPKNCQHAVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSH
                     CWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKL
                     AARIHDHDRWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVRE
                     KLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1170 a         1065 c         1037 g         1321 t
ORIGIN      
        1 attgtttacc atttgctccc aaaatgcgtc ctggttcttt cttaactgct gccatttttc
       61 cgccagactt tgcacggtaa tttgctgcca cagacgtttc gaaagttgat atgtctcttc
      121 cggcgacaag aacggcgtat tatttaatga gctggatgcc aggcgactgg tcagcatacg
      181 taaatcacgt tcagcttgct gatcaaccgc atttttcagc caggtgaggc gggtagattt
      241 gacatcatcg agttcttcag cagaaaaacc atgctgatca atggttgcca actctgccat
      301 taatgcattt gcagcatcct gcatattatc gtctcgtgca tttacacgga aaaacagcga
      361 ctgataatcg ggtgcaattt taacgctgcg cgcagtgccg ccagaaatag tcttcaactc
      421 gcccgactgt atgcgttcct gcagacgttg attgaacagc tgaactaaca tgctccattc
      481 agcttgttcg acaaagcttt gctcatcgtt cacttgtacc attggcaggc gataatagag
      541 tgcgatgccg ttcactcggt tttctttatc attaatgata ttaaagcgca ggtggttttc
      601 ggcttttgtc ggccagacgc gattttcagc tgctttgtta gccggaagct tacttaaatt
      661 atcctttatc agcgccagcg cttctttact gtcgatatcg ccgaccacga taaaggtcat
      721 attatttggt tgataccagc gttgataaaa ttggcgcaat tgtgccggtg tgaccgtggc
      781 gacagtatcc atcaggccga taggttcacg gtctaaatta cgggtatttg ccagcaggaa
      841 agggcggcgc gcctgagagg tgcgccattt cgcatcctga tgggcacgcc attcctcagt
      901 aattacgccg cgttcagcgt ctacttcgag tatttcaaag gttgcggcat tactccattc
      961 actgaagatt gccatcactt gttgcagatt ttgtttctgc gtagtcggca gactcacctg
     1021 atacagcgtt tcgtcatagc tggtataggc attaacatcg cgaccgaaac gcaggcccat
     1081 tgactcaaat gtttcgatga ctttattacc cggccatgtt tttgtgccgt taaacatcat
     1141 atgttctaca aaatgagcca cgccgcgctc attgtcttct tcctgcaatg aaccggtatg
     1201 aatttgcagc cataaattta cctgatcctt tggttgagca tgcggataaa tcatatatcg
     1261 caagccattg tccagttgcc cggtaattaa cttttcatcc tgcggtaagg cggcggcaat
     1321 cagccgcccg gggagcaaca gagttgccac taacgtcagt aagaaacaga ggtttctcat
     1381 aattatctcc atgcgaaaac cgggcgaatt tacccggtta agtaaaatcc gaactattaa
     1441 aatttcatgc tgacatcaag ccagaaagta cggccactgg cgtaggtcgc cacaccggtg
     1501 ttcgttgtat caacagcggt tttgctatcc agtacattga gaatatcggc actgatcgtc
     1561 aggttttgtt gtttcagaaa ttgtggcgtc caggacaagc gggtgtccca ggtcaggctg
     1621 ctatccagtt tttcgtcaac atactgcttg taatctgaat attcgctgat gtattgcgca
     1681 tttgtcttac caaggataat gcgagcttta cgcgcttctt gccaggccag cgtattcgcc
     1741 cacactaacc cgctcggttg atgcgtgaaa tccatgttta aggagatctt taatgggtta
     1801 ttaaaatctg ccactggaac actatcgtaa gagaccagat taccgttata aaccacctgg
     1861 ttatcaccgg tattgctctc ctcataacca ttgtttaacg acaagttgcc cttgctcttg
     1921 atatagctaa agacaatttg tgggttaata tctacttggc ggatatgcag gggttcggcc
     1981 agttcaaaac tgaggctgaa cgaatgcgtt ttggttttgc catcgttgtt atattcagta
     2041 atggtggttt tagtcgcgct gtcggtacga ctgcttttgc tgatttgatc atgcgcttca
     2101 cggtaaacat agtttgcacg cgcaataacg ttcttaccga ttttctgctg caatcccatt
     2161 gccagttcat cgttataagg cgttttcaaa tcctgataac gcgtcagagt tttattacct
     2221 gataccgatt ccgtccagct attgcggata tcacgtaatc ccatatcaag aatattcccg
     2281 ccatagtaac ggttataacc ggcggtaatc attgaggttt gatcagcaaa aatatcccat
     2341 tccgtcataa agcgcgggga gatattgtgg tttgacagat agttgtcata gtcataacgc
     2401 acaccgggca ttaacgacac attccgccag ctaatgtgct ccgccatata cagcgtgtag
     2461 ttgtcaattc ccaggctgcc tttaccttta tggtaaatgg tatggttagt tttctttcct
     2521 gcagcattaa tcacatagga ttcagactgg ttatggcgtt cagtccatgc atcggaatag
     2581 atgtattccg cgccgaagta gggttgatgc gaaacatcac ccacggcgaa tttttgccag
     2641 tccaggcgtg ttttgaaggt gtaattatct acagcctggg aaatgtgtcc taatccgcca
     2701 caggtacaac gccctgtaat atcaccatat gtacatgaaa gttcggtgta ccagatgtca
     2761 tgatcgtgac gggtataatc actaatatga tcccaaccaa cggtggtacg tagtttggcc
     2821 catgcgagtt gcgtatccat atcccatgcc agaccatagg atttattacc catttcgcga
     2881 tcagactgcg ggaaggtgct ggtattataa tcacggctgg agccggtata ttttaaggtt
     2941 aaatcgtggg taaagcggtc gctggcaaac caggtaaatt tgctcaatgc agtatcgata
     3001 acgtttttat actgtgcccg accggtgaca atgccgtcat tcgaaacata atccgcgcgg
     3061 gtgatatcag actggcggcg cgataaaccg gcggtaacgc cgaagttatc agcgagttcc
     3121 tgattaaacg acaaggtata aaagttcttt ttaaaatctg gggagtaata agtacttcct
     3181 gaagagcctt gattaaatgc gctcttgtta ttctcatcga tatgcgatgt taaccagtcc
     3241 gaacgcgtag tgcgataacc caatttcacc ttgctatcat cagcgttgaa gcgtttgatc
     3301 tttgcatcaa ttaccccgcc attgaagcga ccaaattcaa ccggcacaaa actgtcataa
     3361 agcgtcacat tgtccagtaa gctgacatca agataatacc cctgtgacat cccgctaata
     3421 ttggttgcac tactggcatc ggactcattc gctgggttca ggttattagt tgcactaata
     3481 ccgtcaatca aataggcatt ctggtagggc gacgcaccgt gaatagagat tttctcaggg
     3541 cgaatatctc cctggttcaa cgaggtactt tgcgttgaat ccatgcgtac cgcagggttg
     3601 gttctcagca gatcgctgat attgccattc ccggtcggta aacgctcgat gctttcactg
     3661 gtgtaatgcg tgttgccgtt tacgggtaca gggacaggag catagaccgt catctcttca
     3721 aaaaaatgca tgtacatgtt tttgtcatcg acggcctgcg ccacatcagc gccacataaa
     3781 atgacgccag gaataagaac tcgcttcatt tatttatatc tccgtacagc agggctttta
     3841 ttgttgttat aaaaccgtgc taatgtcaca aatatcatca gccaggttcc agacaccggg
     3901 ttgatgtgta accataatga cgccgcttgt gggtagtttt tcacgcacta aacgcagtaa
     3961 gcgtatagcc tcttgttcct caagatgaga ggtagtttcg tcaagaaata tccatttcgg
     4021 acgtcgtaaa attaatcgtg ccagggcgat acgttgtttt tcgccgctgg aaagaatatc
     4081 tccccagcga tcgtggtcat gaatacgcgc agccaatttc cccagaccaa cctgatgcag
     4141 tacttcgctc aacgatttat cgtctacggg caggggaagt gctttacaaa taatctcttt
     4201 cagtaagccg cttttgatta acggtgtttg tgacacatac caactgtcag caggagaaga
     4261 aatatcacct ttaaaccacg gccagcagtg ggataatgtt ttaagcagtg tggtttttcc
     4321 cgcaccagag tagcctttca gtaatagcca tttgcctggc gaaacatgaa agttcaagtt
     4381 ctctaatatg atcttattat caggcgtacg aatactcgca ttagccactt gtaccgcatg
     4441 ttggcaattt ttaggcttat tcgtagggcg ctgttcagtg agttgatgga actcatacaa
     4501 gcgatcgata accgcagcca gttcagcaag ttcgtcatat ttataaataa accagcttaa
     4561 attgttcgat actagcataa atgcctggcg cga
//
LOCUS       DAZPJP010000266         4490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPJP010000266 DAZPJP010000000
VERSION     DAZPJP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.269"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHU9J_004070"
     CDS             <1..461
                     /locus_tag="ACHU9J_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1907478.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHU9J_004071"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHU9J_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1907479.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..>4490)
                     /locus_tag="ACHU9J_004072"
     CDS             complement(2231..>4490)
                     /locus_tag="ACHU9J_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1907480.1"
                     /translation="NVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSV
                     GKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSG
                     FGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGI
                     NALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSI
                     GVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSAR
                     TYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQ
                     NRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFA
                     PSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDK
                     EGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETW
                     QAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDWE
                     GISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYF
                     GAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQD
                     LPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSAR
                     GRTWIFGGDIRF"
BASE COUNT         1140 a         1050 c         1046 g         1254 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc
//
LOCUS       DAZPJP010000267         4483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPJP010000267 DAZPJP010000000
VERSION     DAZPJP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.270"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHU9J_004073"
     CDS             complement(8..1069)
                     /locus_tag="ACHU9J_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1907481.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHU9J_004074"
     CDS             complement(1066..2463)
                     /locus_tag="ACHU9J_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1907482.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHU9J_004075"
     CDS             2618..3616
                     /locus_tag="ACHU9J_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1907483.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHU9J_004076"
     CDS             complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="ACHU9J_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR1907484.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gcc
//
LOCUS       DAZPJP010000268         4464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPJP010000268 DAZPJP010000000
VERSION     DAZPJP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.271"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHU9J_004077"
     CDS             complement(<1..264)
                     /locus_tag="ACHU9J_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1907485.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="ACHU9J_004078"
     CDS             493..1101
                     /locus_tag="ACHU9J_004078"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1907486.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="ACHU9J_004079"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="ACHU9J_004079"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1907487.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="ACHU9J_004080"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="ACHU9J_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1907488.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1000 a         1136 c         1322 g         1006 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaataaa ttaa
//
LOCUS       DAZPJP010000269         4411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPJP010000269 DAZPJP010000000
VERSION     DAZPJP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.272"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..107)
                     /locus_tag="ACHU9J_004081"
     CDS             complement(<1..107)
                     /locus_tag="ACHU9J_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907489.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(175..2124)
                     /locus_tag="ACHU9J_004082"
     CDS             complement(175..2124)
                     /locus_tag="ACHU9J_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1907490.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2209..2544)
                     /locus_tag="ACHU9J_004083"
     CDS             complement(2209..2544)
                     /locus_tag="ACHU9J_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1907491.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2544..2900)
                     /locus_tag="ACHU9J_004084"
     CDS             complement(2544..2900)
                     /locus_tag="ACHU9J_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1907492.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2900..4396)
                     /locus_tag="ACHU9J_004085"
     CDS             complement(2900..4396)
                     /locus_tag="ACHU9J_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1907493.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKQGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1313 c          970 g         1108 t
ORIGIN      
        1 ttaacgttac taataaatcg tgaaacaatc tcctcatttg ttagtatttt cgcctgagca
       61 cagcaagagg ttaatattgc aaccgtcatt aaaaatggct ttatcacaaa acaaactcca
      121 ttgttagcat gttacgcgat aattttgcct catctttcac cacaaaaaaa gaactcacct
      181 tttaaatgga gaatacccaa catcatgggt gattttcata aagggatcgc ctgttttcgg
      241 caaatcgata acgcgcattc caggcggcgc gttgtcaaac gtcaccttaa gttcgctgcg
      301 ggttgccggt gaagatgcaa ggcttaacag ctgattcctt tccagtgata cattcggggt
      361 gtagcgttta tcgggagata aatacccatc accacgtggc atctgccatc cggtcatgtc
      421 atagacaaaa tcatggaact gtttgcccca ctcgtcgaac ttatcattca gaccaaatcc
      481 actgttcagt gcatcagcca caaaattttt aacaagccag ggatgtgctt tttcaaactc
      541 ctgcgccatc atgcccagtt caagcaatcc accaatcaga ctgatctttc cgaggccctt
      601 taatttcagc cctgtttttc ctgccgccgt tgtccattga ttctgggcga ctgttgccgc
      661 acgaatactt gccaccgcct tcacaccaat atacagggca gaaaccgttg ctattgtttt
      721 tactgcctgt tcccatcccc ccattgctct gacaacctga tcaacctcct gccagacttt
      781 tttcacaacc ggaccgaccg tttcccagtt atcaattatg agatatgcac caccgacgag
      841 aagcgcaatt aatccctttg ccggcgtcat attcatcacg ctacccatga tttttgttat
      901 gcgtgtcaat gtgccaatcg ccacgcccat cgtcagtaat gccgcaccag ttttcgcgat
      961 ggtttttacg acctcaggat tttctctgac aaacgtgcgc acttcttcca gaaaaggctt
     1021 catctctttt atgccttcat tgagtgaagg caggaaggtt tcccccagcg tggaagaaat
     1081 cgcattgatc tggttctgca gcagcaacaa ctgattttct gtcgtcgcgg cacgggcggc
     1141 atattctttc tgcattgagc cgccatactg ctgcgcatcc gccacacgat taaagttggt
     1201 tcgcaacaaa tccagattcg tgagcagcgg cgcaatggcc cccagggatt ccttcccgaa
     1261 cagcgctttc agcacagcag cctgtttttc tttcggcact tttgccagag aatccagtac
     1321 gtgcagcatg gccccgcggg cgtctttctg catatccgcc gccagttttt tcgggctaat
     1381 gcgcaacgag cggagcactt ttttctgcga tttggtggca gaatccctcg ctgtcagcga
     1441 caacataaaa ttctttatcc ccgtcgcagc aatttctgac tccaccccca ttccggcaat
     1501 ggttgccccc attgcggcaa tctctccgga ggccacaccc gcaacactgc ctaaagggcc
     1561 aatacgggtc acaacatcag aaatcttttt cgcacttgca ggaccggtat taccaagata
     1621 gttaatctta tccgcaagcc ctgccacctc tccctgtgtc agtttaaagg cagtgcgcca
     1681 ctgcgccatc atctgaccgg attcttctgc cgtggtgtca aaggccacgc ccatcttcac
     1741 ggcatcgtca gtaaactgca tcagctcgtc acgggcgata ccagcctgac cacccgccgc
     1801 cacaatttcg gcgatacctt ccgccgacat gggcagttct gttgacaggt cgcgcacctg
     1861 ctccgtcatt gccttaaacg cttccggcgt atccagacca tccaccactt tccggacatc
     1921 cgccattttt gattcaaggg caatggcaga tttgaccggg agcgccagcg cccccagtac
     1981 tgcggttccg gcacccgctg cgcccagtaa caggctggca aactcctttt taaaaccttt
     2041 cagctggcgc tgcattccct tcagcggtgc cgatacctta tccacggcag tgatgatcgc
     2101 cttcagctga aaactgtcag ccatgcttca tctcctcgtt aatgcggacg gcctcggctt
     2161 ccagttctgt aaactgcgaa acagcccccc gtcgcagctc cagtggattc agtttccaga
     2221 accaggcaac attgtagagt cgtttccgga ggtgcttccc gtctccgact gggtaaaaaa
     2281 acgcaggatc tgcatgctgg tcttaaaaat atccagtttt gccatctgcg ccgccgatga
     2341 acgcgggatc cccgccagca acgggatata tttcagtgcc acctggctgt ccagtttaat
     2401 actgccttca ccggaaataa tgaaaggaaa acccagcgcc tcgatttcgt catacgtggg
     2461 ctcgcgcaac tccagcacat gcagcatttc gttatgcgct gtcaccggtt tttttaaaac
     2521 tatttctgta acgttcattg ccattactga taccctccct cttcgccgtg gaattcaaga
     2581 tctgccgtgc cttcttcggc attatgatta gcctctccgt gcagccatgc cgccgaaagc
     2641 acatacacca tgccgtttgc cagctcagcg gtaatcgtca tctggtctga ggtggtaatt
     2701 ttgtcgaccg gaaagttttt gggcacttta aacgtgccct taacgtaagg tgaccgccag
     2761 gtctccttgt aatccacatc ccctgccatg ccgacgatgt catcccggac attggtgttc
     2821 atcggcactt caatgccacc tgtcagcgat aactgctgac catccacttt aaaaaaacag
     2881 gttccggcga tctttgccat tatgctgact cctctgaata ctgaagacgg aactggttaa
     2941 ccaccgcaaa gacacgtaac tgattaacat aatccggcgg gaacagcgta ttcagtcggt
     3001 tcgggttatc tgcatcacgc tcaactatca gatactgttt aaacagatcg taattttcca
     3061 caataccggc acgctccatc tgacgatatg tcgccagaag ttcccctttg ataacggcag
     3121 gagtgacaat cgcctgcccc ggaccaaaac gggtaccatc atttgccagc ttgtgacgtc
     3181 cgtacttgct tgtgatgacc gatttcagtt tgcgaagaac atatgcactg gtatgcagag
     3241 tttcactgtc cagatagctg ttgtctgcca cgccatacgc attctttttg taggtggtta
     3301 cggaacgctg gatctgcaac gtgccgcctt ccacataagc cgtcgccacg ccgtgagata
     3361 aaagggtctg ctgctctgtc atgatgaatc gcttaccttt cggtgccgga agcatcccca
     3421 ccagctcccc cgtctgtgtc ggacgggcag gatcattccg gataaatacc gcttcacggg
     3481 caaggcgact ggcaaccagt tcatcgacag gcgactgggt ttctttttcg taaccggcaa
     3541 gcgtgatatg ttgctgatta tgcatatctc cggcaccaac cagctctgac agcgttccct
     3601 gttttgcggt atagacatgc ccgtataact ggcgcgcata actccagcga ccgctgctgt
     3661 cattcatttc ggtcatcatc atattgatgg aggcggcatc gttgaacggc agaccgataa
     3721 agtcgaatgc ctcatcgccc atagcagcaa cagcggcggt aagatcaggc gctccgctac
     3781 cggcagcacc gacttccgtc accacccgaa gccctgctgg cagaatctca ccaccaccag
     3841 aaccataata attcagacag accggtaact cattaccata cagcccctta tgacgggcag
     3901 tcagcgtcac cacacctgca tcagatgacg ccgtaaacgg cagggtgata accccgttta
     3961 ccgcttcctt aatcgcggtg gcaaccgcag tggcatcatc gccattcacc acaggcacct
     4021 gtacacggga gcgcccgaca tacagactca gggtgccgct ttcctctgct tctccggcaa
     4081 ccgtcaccct gaccgtcgcc gccgcccctc tggcttccgg tactgcaata acatacagtt
     4141 caccgaaagg atctgtctga cggtagactt ccaccatacg cgccagctgg ctccccgccc
     4201 cacaaatctg gcgggcataa tctgccgatg gcatcagcac caggctgtta acctcaatgg
     4261 ctgcatcgtt gctggcatgc ccgatcagta atgcaggcgc gctggtcacc gctgtatttg
     4321 ccgcagaatt gttcatctcg gcgtagaaca acggcaccag cgtattcgac ggaacagtat
     4381 taaaacttat cgtcatgatt ttttagcctt a
//
LOCUS       DAZPJP010000270         4352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPJP010000270 DAZPJP010000000
VERSION     DAZPJP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.273"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHU9J_004086"
     CDS             complement(<1..215)
                     /locus_tag="ACHU9J_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1907494.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(318..393)
                     /locus_tag="ACHU9J_004087"
     tRNA            complement(318..393)
                     /locus_tag="ACHU9J_004087"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Thr,seq:ggt)
     gene            complement(400..474)
                     /locus_tag="ACHU9J_004088"
     tRNA            complement(400..474)
                     /locus_tag="ACHU9J_004088"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Gly,seq:tcc)
     gene            complement(591..675)
                     /locus_tag="ACHU9J_004089"
     tRNA            complement(591..675)
                     /locus_tag="ACHU9J_004089"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Tyr,seq:gta)
     gene            complement(684..759)
                     /locus_tag="ACHU9J_004090"
     tRNA            complement(684..759)
                     /locus_tag="ACHU9J_004090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Thr,seq:tgt)
     gene            1121..2071
                     /gene="coaA"
                     /locus_tag="ACHU9J_004091"
     CDS             1121..2071
                     /gene="coaA"
                     /locus_tag="ACHU9J_004091"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1907495.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2100..3065)
                     /gene="birA"
                     /locus_tag="ACHU9J_004092"
     CDS             complement(2100..3065)
                     /gene="birA"
                     /locus_tag="ACHU9J_004092"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1907496.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3062..4090)
                     /gene="murB"
                     /locus_tag="ACHU9J_004093"
     CDS             complement(3062..4090)
                     /gene="murB"
                     /locus_tag="ACHU9J_004093"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1907497.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="ACHU9J_004094"
     rRNA            complement(4225..4340)
                     /gene="rrf"
                     /locus_tag="ACHU9J_004094"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1106 a         1093 c          981 g         1172 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gcggtgatat
      241 caccacatca accaggcaac atgcccgact tgttgaatgc aataaacaga aggaaaaaac
      301 agggaggaga aaagaagtgg tgctgatagg cagattcgaa ctgccgacct cacccttacc
      361 aagggtgcgc tctaccaact gagctatatc agcacatctt ggagcgggca gcgggaatcg
      421 aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc ccgcatcctg
      481 gaactcggct acctgatttt cattctgcac tgaatatcga gagaagctct ctttattcga
      541 gccggtaagc gaacttatcg tctcgggcta cgccatcgcg tggccgaaat tggtggtggg
      601 ggaaggattc gaaccttcga agtctgtgac ggcagattta cagtctgctc cctttggccg
      661 ctcgggaacc ccaccggact tgatggtgcc gactaccgga atcgaactgg tgacctactg
      721 attacaagtc agttgctcta cctactgagc taagtcggca tcaagtagcg cgcattctat
      781 ggagacatgc gagttcatgc aactaaaaaa ttgcataatt tgttttattg gtcacatttt
      841 atgcgacacg atgaagaaac agccgaacgg agggcagatt agcacacttt ttcaacatca
      901 ttgtgctcaa caatgcgctc ctgctaaacc ataattcttt ttatcagatg gaatatctgt
      961 cacattgctt ttcaacgata gcttcctggc agagattttt tcttattatt cctccccatc
     1021 tggtgttacc ctcctgccca ttaacccatt caacagaact gtgacgcgcc atggcaaata
     1081 tcgctttgcc gatagagcta tgaccgccag aaacatgctt atgagtataa aagagcaaac
     1141 gttaatgacg ccttacctac agtttgaccg caaccagtgg gcagctctgc gtgattccgt
     1201 acctatgacg ttatcggaag atgagatcgc ccgtctcaaa ggtattaatg aagatctctc
     1261 gttagaagaa gttgccgaga tctatttacc tttgtcacgt ttgctgaact tctatataag
     1321 ctcgaatctg cgccgtcagg cagttctgga acagtttctt ggtaccaacg ggcaacgcat
     1381 tccttacatt atcagtattg ctggcagtgt cgcggtgggg aaaagtacaa ccgcccgtgt
     1441 attgcaggcg ctattaagcc gttggccgga acatcgtcgt gttgaactga tcactacaga
     1501 tggcttcctt caccctaatc aggttctgaa agaacgtggt ctgatgaaga agaaaggctt
     1561 cccggaatcg tatgatatgc atcgcctggt gaagtttgtt tccgatctca agtccggcgt
     1621 gccaaacgtt acagcccctg tttactcgca tcttatttat gatgtgatcc cggatggaga
     1681 taaaactgtt gttcagcctg atattttaat tcttgaaggg ttaaatgtct tacagagcgg
     1741 gatggattat ccacacgatc cacatcatgt atttgtttct gattttgtcg atttttcgat
     1801 atatgttgat gcaccggaag acttacttca gacgtggtat atcaaccgtt ttctgaaatt
     1861 ccgcgaaggg gcttttaccg acccggattc ctattttcat aactacgcga aattaactaa
     1921 agaagaagcg attaagactg ccatgacatt gtggaaagag atcaactggc tgaacttaaa
     1981 gcaaaatatt ctacctactc gtgagcgcgc cagtttaatc ctgacgaaaa gtgctaatca
     2041 tgcggtagaa gaggtcagac tacgcaaata atttgcaggg gagcgaatac tcccctttct
     2101 tatttttctg cactacgcag ggatatttca ccgcccatcc agggttttat tattccatcc
     2161 tgctcaagta ataaagcccc ctgtttgtct attccgcgtg aaatgccaaa tatttcttta
     2221 tcaccaatga taagtttcac tgggcgatta ataaaattat ccagcttttc ccagcgcgac
     2281 agataaggtg ccaatccttc ttgttcgaag agttccaacg cagcacgtaa ttcacgtatt
     2341 agcatggccg ccaacgtatt acgatcgaga ttgatccccg cttcctgcag cgtgatccac
     2401 ccctgattaa cgacactctc ttcaacacgg cgcattgcca tgttgatccc ggctccaatg
     2461 actatttgcg ccgcatcgcc agttttgcca gtcagctcca caagaatgcc tgccagcttg
     2521 cgatcctgca gatagaggtc attaggccat ttgacacgaa ctttatctgc tcccagcttg
     2581 cgtaatactt ccgccatcac gataccgata accagactta aaccaatcgc cgccgccggg
     2641 ccttgttcca gacgccagaa catcgacaaa tataagtttg cgccaaaagg cgaaaaccat
     2701 ttccgccccc ggcgaccacg gccagcctgc tggtattctg caacacaggc atcgcccgat
     2761 ttaagctctc cgatacgatc aagaaggtac tgattcgtgg agtcaataac tggcagcacg
     2821 gctacactac cgccatccag ctgacccaat atctgttcag cattaagtaa ctggatgggc
     2881 tcaggcaggc tgtatccttt acccggaacg gtaaagacat caacgcccca gtcacgcagt
     2941 gtctgaatgt gtttattaat agccgcccgg ctcattccca gcgtttcacc caactgctcg
     3001 ccagagtgaa attcaccgtt cgctaacagg gcaatcaatt tcagtggcac ggtgttatcc
     3061 ttcatgaaat tgtctccact gcgctcactt cacctgatgc accaataaag cggacttcag
     3121 gctcaagcca gacattaaat ttttcaccca ctttttgtct tacatggtgt gccagttgca
     3181 ccacatcttc gctttttgca ttgtcttcat taatgagaac taacgcctgt tgacggtgca
     3241 ccgcagcccc acccatttgc atccctttta gctggcactg atcaataagc caacctgctg
     3301 ccagttttac tgaaccaccc gcctgggggt aatttggtgc tgttggaaat tgcgccagta
     3361 atgctttagc cgtttcggca gatacaacag ggtttttgaa gaaactaccg gcattgccat
     3421 tcacttttgg atcagggagt ttggtggtgc gcatatgaca caccgcatta aatacttgct
     3481 gtggcgttac tgttgtagga tccagacgag ttaagtcacc atacgttagt acaggttgcc
     3541 actcttttgg cagacgcaga cctacggcta caatggcgaa gcgatcctgg tattcatgtt
     3601 taaaaatact gtcgcgatag ccaaaacggc actctttggc agttaagcgc acttgcttgc
     3661 ctgtcgccag ttcaacacaa tcaacataag cgcaaactcg ctgtaattct acgccataag
     3721 caccaatatt ctggataggt gatgagccga cacaaccagg aattaatgcc agattttcca
     3781 gaccaggcat accttcctgc aaagtgtatt ttaccaggcg atgccagttt tctccggctc
     3841 ctacatgtaa ataccacgca tcaggttcat catgaatttc gatacctttg atccggttga
     3901 tgatcaccgt gccacgatag tcttccagaa aaagtacatt acttccttca cccagaataa
     3961 gaacgggttg tccttctgcg gttgcatgct gccaggcatt gagtaattgt tgttcgtctt
     4021 cggcacatac aatgtgctga gcattatgat caatgccaaa tgtgttccag ggttttaagg
     4081 agtggttcat agctgctttc ctgatgcaaa aacaaggcta gtttaccgta tctgtggggg
     4141 gatggcttgt agatatgacg acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4201 ggatggcctt ctgcttaatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc
     4261 acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc
     4321 accgcgctac ggccgccagg caaattctgt tt
//
LOCUS       DAZPJP010000271         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPJP010000271 DAZPJP010000000
VERSION     DAZPJP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.274"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHU9J_004095"
     CDS             complement(<1..583)
                     /locus_tag="ACHU9J_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1907498.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHU9J_004096"
     CDS             complement(646..1095)
                     /locus_tag="ACHU9J_004096"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1907499.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHU9J_004097"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHU9J_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZPJP010000272         4318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPJP010000272 DAZPJP010000000
VERSION     DAZPJP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.275"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            130..3987
                     /gene="sigA"
                     /locus_tag="ACHU9J_004098"
     CDS             130..3987
                     /gene="sigA"
                     /locus_tag="ACHU9J_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1907500.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            4046..>4318
                     /locus_tag="ACHU9J_004099"
     CDS             4046..>4318
                     /locus_tag="ACHU9J_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907501.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
BASE COUNT         1409 a          863 c          929 g         1117 t
ORIGIN      
        1 tctgtcatac aaatataact gacagtgatt atcattatac ccttatcagt tacgtaccat
       61 gactgatagt tccccgttgt aattaaatgc tatcccataa ccacaactca gaaatatcgg
      121 agttcacgta tgaataaaat ttattcactg aaatatagtc atattacagg tggattagtt
      181 gctgtttctg aactgacccg gaaagttagt gtcggtacat caagaaagaa agttatcctc
      241 ggtattattt tatcctcaat atatggaagt tatggcgaaa cagcatttgc agcaatgctg
      301 gatataaata atatatggac ccgcgattat cttgaccttg ctcaaaacag aggagagttc
      361 agaccgggtg caacaaatgt tcaattaatg atgaaagatg gaaagatatt tcattttcca
      421 gaactacctg tacctgattt ttctgctgtt tccaacaaag gtgcaacaac atcaattgga
      481 ggtgcgtaca gtgttactgc gactcataac ggtacacagc atcatgcaat aacaacacag
      541 tcatgggatc agacagcata taaagcaagt aacagagtat catctggcga cttttcggtt
      601 catcgtctga ataaattcgt cgtggaaaca acaggggtta cggagagtgc cgacttctca
      661 ctttctcccg aagatgcgat gaaaagatat ggcgtaaact acaacggtaa ggaacaaata
      721 attggcttca gagcaggtgc cggaacaacc tcaacgatat taaacggcaa acaatatctg
      781 tttggacaaa actataatcc cgacttgtta agcgcaagtc tttttaatct ggactggaaa
      841 aacaagagtt acatttatac caacagaacc ccttttaaaa actcaccaat ttttggcgat
      901 agtggttctg gttcttatct atatgataaa gaacaacaaa aatgggtttt ccatggtgtt
      961 accagtacag ttggttttct cagtagtacc aatatagcct ggacaaacta ctcgttattt
     1021 aataatattc tggtaaacaa tttaaaaaag aatttcacaa acactatgca gctggatggt
     1081 aaaaaacaag agttatcatc gattataaaa gataaggacc tgtctgtctc aggaggaggg
     1141 gaattaacgc tcaagcagga taccgatctt ggcattggcg ggcttatatt cgataagaac
     1201 cagacatata aagtgtacgg aaaagataag tcttataaag gtgccgggat agatattgat
     1261 aataatacca ccgttgaatg gaatgttaag ggcgttgccg gagataatct gcataaaata
     1321 ggtagtggta ctctggatgt aaaaatagca cagggaaata accttaaaat aggtaatggg
     1381 actgtcatcc ttagtgctga aaaagccttc aataaaattt acatggccgg aggtaaaggt
     1441 acggtaaaaa taaatgccaa agacgcttta agcgaaagcg gtaatggcga aatctatttt
     1501 accagaaatg gcggaacact ggatctaaac ggctatgacc agtcatttca gaaaatcgca
     1561 gcaacagatg cgggaacaac cgtaacgaac tcaaacgtga agcaatcaac attatcactt
     1621 actaatactg atgcatatat gtaccatggg aatgtatcag gtaatataag cataaatcat
     1681 attatcaata ctacccagca acataacaat aatgccaatc tgatctttga tggctcagtc
     1741 gatatcaaaa acgatatctc tgtccggaat gcacagttaa cattacaagg acatgcgaca
     1801 gaacatgcca tatttaaaga aggcaataac aactgtccaa ttcctttttt atgtcaaaaa
     1861 gactattctg ctgccataaa ggaccaggaa agcactgtaa ataaacgtta caatacggaa
     1921 tataagtcca acaatcagat agcctctttt tcccagcccg actgggaaag tcgtaaattt
     1981 aatttccgga aattaaattt agaaaacgca accctgagta taggccggga tgctaatgta
     2041 aaaggacaca tagaggctaa aaactctcaa attgttctgg gaaataaaac tgcatacatt
     2101 gacatgttct caggaagaaa cattactggc gaaggttttg gattcagaca acagcttcgc
     2161 tccggggatt cagcaggcga aagtagtttc aacggcagtc tgagtgctca aaacagcaaa
     2221 ataactgttg gtgataaatc aactgttact atgactggtg cattatcctt aattaataca
     2281 gacctgatta tcaacaaagg agctactgtt accgcccagg gaaaaatgta tgtagataaa
     2341 gctattgaac tggccggaac cctgacatta acaggcaccc ctacagaaaa taataaatac
     2401 agcccggcaa tctatatgtc agatggatat aatatgacag aagatggtgc cacgttaaag
     2461 gctcaaaatt atgcctgggt caatggtaat ataaaatcag acaaaaaagc atctattctg
     2521 tttggtgttg accagtataa agaagataac ctggacaaaa ccacacacac accgctggct
     2581 acaggtttgc tgggtggctt tgatacttct tataccggag gtattgatgc tcctgctgcc
     2641 tcagccagca tgtataacac cttatggaga gtaaacggac agtcagccct gcaatcatta
     2701 aaaacccgcg acagtctttt gttgtttagt aacatagaga attcgggttt ccatactgtg
     2761 acagtaaaca cactggatgc cactaatact gctgtgatta tgcgggctga tctgagccag
     2821 tctgtaaatc aatcggataa actcattgtt aaaaatcagt taaccggaag caataacagt
     2881 ctgtcggtcg atatacagaa agtgggaaat aataactcag gattaaacgt tgacctgata
     2941 acagccccaa aaggaagcaa taaagagata tttaaagcca gtactcaggc cataggtttc
     3001 agcaacatat ctcctgtgat cagcacgaaa gaggatcagg aacataccac gtggaccctg
     3061 accggatata aggtggctga aaatacagca tcttccggtg cagcaaaatc gtatatgtcc
     3121 ggtaattaca aagccttcct gacagaagtc aacaacctga ataaacgaat gggggatctg
     3181 cgtgacacca atggcgaggc cggtgcatgg gcccgcatca tgagcggagc aggttcagct
     3241 tctagtggat acagtgacaa ctacacccat gtgcagattg gtgtggataa aaaacatgag
     3301 ctggatggac ttgacctttt cactggtctg actatgacgt ataccgacag tcatgccagc
     3361 agtaatgcat tcagtggcaa gacgaagtcc gtcggggcag gtctgtatgc ttccgctata
     3421 tttgactctg gtgcctatat cgacctgatt agtaagtatg ttcaccatga taatgagtac
     3481 tcggcgacct ttgctggact cggaacaaaa gactacagtt ctcattcctt gtatgtgggt
     3541 gctgaagcag gctaccgcta tcatgtaaca gaagactcct ggattgagcc gcaggcagaa
     3601 ctggtttatg gggccgtatc aggtaaacgg ttcgactggc aggatcgcgg aatgagcgtg
     3661 accatgaagg ataaggactt taatccgctg attgggcgta ccggtgttga tgtgggtaaa
     3721 tccttctccg gtaaggactg gaaagtcaca gcccgcgccg gccttggcta ccagtttgac
     3781 ctgtttgcca acggtgaaac cgtactgcgt gatgcgtccg gtgagaaacg tatcaaaggt
     3841 gaaaaagacg gtcgtattct catgaatgtt ggtctcaacg ccgaaattcg cgataatctt
     3901 cgcttcggtc ttgagtttga gaaatcggca tttggtaaat acaacgtgga taacgcgatc
     3961 aacgccaact tccgttactc tttctgataa cagcccgggc cgcgtttgcg gcccttcttc
     4021 taccgtagag aatatgtatt accctgtgac agactatatc gctcttgctc tcattattag
     4081 ctttcttttt ctgacattat ttatctgcct gttatgcctt aaacatgagc gaataaaaaa
     4141 agaaactatc aggcaaaaaa atgcacatat tctggagcat ggctggaatg caactgagtt
     4201 ctcatggttc cgatacggac agtataacga aacgggtatt tacatctcga tcgagaagac
     4261 cattatcatt acaatcaccg tttctggtga gtgctttaaa aaagaataca gcattgtc
//
LOCUS       DAZPJP010000273         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPJP010000273 DAZPJP010000000
VERSION     DAZPJP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.276"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHU9J_004100"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHU9J_004100"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1907502.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHU9J_004101"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHU9J_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1907503.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHU9J_004102"
     CDS             2269..2976
                     /locus_tag="ACHU9J_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1907504.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHU9J_004103"
     tRNA            3082..3157
                     /locus_tag="ACHU9J_004103"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHU9J_004104"
     CDS             3355..>4309
                     /locus_tag="ACHU9J_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1907505.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPJP010000274         4256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPJP010000274 DAZPJP010000000
VERSION     DAZPJP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.277"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHU9J_004105"
     CDS             complement(<1..3511)
                     /locus_tag="ACHU9J_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1907506.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4256)
                     /locus_tag="ACHU9J_004106"
     CDS             complement(3963..>4256)
                     /locus_tag="ACHU9J_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1907507.1"
                     /translation="NKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1096 a         1094 c          877 g         1189 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatt
//
LOCUS       DAZPJP010000275         4251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPJP010000275 DAZPJP010000000
VERSION     DAZPJP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.278"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            125..277
                     /gene="hokA"
                     /locus_tag="ACHU9J_004107"
     CDS             125..277
                     /gene="hokA"
                     /locus_tag="ACHU9J_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1907508.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(254..325)
                     /locus_tag="ACHU9J_004108"
     CDS             complement(254..325)
                     /locus_tag="ACHU9J_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907509.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(465..677)
                     /gene="cspA"
                     /locus_tag="ACHU9J_004109"
     CDS             complement(465..677)
                     /gene="cspA"
                     /locus_tag="ACHU9J_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1907510.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(958..1248)
                     /locus_tag="ACHU9J_004110"
     CDS             complement(958..1248)
                     /locus_tag="ACHU9J_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1907511.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1682..2392
                     /locus_tag="ACHU9J_004111"
     CDS             1682..2392
                     /locus_tag="ACHU9J_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1907512.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2442..3416)
                     /gene="ghrB"
                     /locus_tag="ACHU9J_004112"
     CDS             complement(2442..3416)
                     /gene="ghrB"
                     /locus_tag="ACHU9J_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1907513.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3520..4179)
                     /locus_tag="ACHU9J_004113"
     CDS             complement(3520..4179)
                     /locus_tag="ACHU9J_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1907514.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1053 a         1026 c         1037 g         1135 t
ORIGIN      
        1 gtattcaccg aaaggcagac agagaaaagc cccacctgac tataaatcaa agtgaggcta
       61 ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac acaggaggct
      121 gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca cgcttttatt
      181 tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg agagttatga
      241 gctggcggca tttttagcct gcaatttgaa agagtaagag tctttggcgg gaagttattc
      301 ccgcctttct tacggcgttg cgcattctca ttgcacccaa atttattctt cacaaaaata
      361 atactaaatt ttattacgcg atcgattatt tatttcctga aaaacaaata aaaaaatccc
      421 cgccaaatgg cagggatctt agattctgtg cttttaagca gagattacag gctggttacg
      481 ttaccagctg ccgggccttt agcgccgctt tcgatggtga aggacacttt ctgaccttcg
      541 tccagagatt tgtaaccatc gttctggata gcagagaagt gtacgaacac atctttagag
      601 ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt tacgatacca
      661 gtcattttac cggacatagt gtattacctt taataattaa gtgtgccttt cggcgatatg
      721 gcgtgcttta cagattttga agcgttaaag gaatgtgcac tacgaggggt atcaacgata
      781 actcttgaag ggacttgcct tactacactg ctttaatggt ctgtacgtca aaccgttgat
      841 gtgcattaag ccacgcattg gcgggtgatg caacaattat ttttcatatt tatgattaat
      901 cggttggaaa acggtcctgt catcaggacc gtaaacagca ataaagtgga taaaagtcta
      961 ttccatcaac tgcttactta atgccgggtt ggcttgaatc aaacgcatca attttagttc
     1021 ggcacttgaa ggcttcacgc gtctggattc ccattccttt accatggcga ctgatacgcc
     1081 caaaacccgg gcgaaatcat cgatttttaa ccctgtccct tttcgtaact gctcaatttc
     1141 ggtacaggac gttgttctgt gcgtcagggt aattttctgc gtttcatctt taaaaacaat
     1201 ctgttccagg ctgctcaaca gctcatgcat tggatcttta tattccattg aaaactcctc
     1261 aaatcacact gcgggatcgt gaattgcatc gaagctcatt aagaatagtc gggaaaagca
     1321 gacacagcgg ggtgaagtca gggcatgctc tggctatgat tcgattcagc ctgtcattta
     1381 gcgtgactta tagccaccta acgatctgat aaatgccgat atatctctta ccactcctgt
     1441 tttgtaaaga tttcgaaaat ctaaccttct gcgaaggcgt aaatctctgg ctacaaaaaa
     1501 ggcaacggca ctatcatttt ttaacaatga aacaacaaat taaccttgtt tcattttctc
     1561 catgatttta ccaggaagat tacgtagaaa gggtatcctg caagcctgtc ctggactatc
     1621 cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa
     1681 aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt tattaatggt
     1741 agttagcctg agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt
     1801 cctgcagaat acagtgatgc gtagcggtga acgtctacca accctgactg ccgatcagaa
     1861 aaaacagttt ggtccttttg tctctgatta cgcgattctg tatggttatt ctcagcaggt
     1921 gaatcaggcg atggattctg gtctgcgccc ggtcgtagac agcgttaacg caattcgcgt
     1981 accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa atgaatggtt cgctgggtgt
     2041 gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat gctgcgcact ctgcgctaaa
     2101 acagagcgat gacctgaaac cggtatttga tcaggcattc accaaagtgg taacgacgcc
     2161 agctgatgca ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac aactggtgat
     2221 ggttggagac tacattgctc agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca
     2281 gttcccgact tcacagcagg caagtgaata taacaaactg attgcgccat taccggcaca
     2341 acatcaggcg tttaatcagg cctggactac agcagttact gcaacccaat aaagagtaaa
     2401 agcccgagcg attatcgggc tttacttcac gcagtcgcgg cttagtccgc gacgtgagga
     2461 ttcacacagt tcttctcaac ctttccttgt aacgcatcaa ttaaattatc cacggcacag
     2521 gcggccatgc cataacgcgt ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg
     2581 ttggccattg agagcaacgg cgaatctacg gacagtggct cttgttcgaa gacatcaagc
     2641 ccggcggcgt gaatttcccc tttctgtaat gctgcgatca gtgcattttc gtcaaccacc
     2701 gggccacgtc cggcattaat gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg
     2761 ccaaacagat gatgcgtctc atcagttaac ggcaggatca ggcaaacgaa atctgactct
     2821 tgtaacagtg tatccaaatc gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg
     2881 tggcggcgcg cgttatagag gatgggcatg ttgaagccaa agtgcgcacg ttgtgccagc
     2941 gccataccga tccgtcccat cccgacaatg cccagtgttt tatggtgaac gtcagtgccg
     3001 taccagtccg ggcctatgct cgcggtccat tcgcctgctt ttacccgttc tgctacctcc
     3061 acaacccgac gagcggtaga caacaccagc gccatcagcg tatcggcgac ggtttctgtt
     3121 aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa
     3181 ttgtcatagc cgacggagat cgttgatgtg gcatgcagtt tcggcatttt ttccagcaat
     3241 gcggcatcaa cattctcgtt tgaacccagt aaaccttcag cttcggcaaa aattgctgca
     3301 ttttgttcga cggtttgtgg gctgaggttt gccacctggt gaacggtgaa atgctcttgc
     3361 aggcgttgca gtaaatcatc aggtaaggct ttgtagagga taacggacgg cttcatgctt
     3421 ctctccattc actgataagg cgatcacctt agcctgaaat atgatgaagg aaaactgaag
     3481 aatgccaggt gatttctgca tcacctggca tgaaagggat tacagcgggc ttaaggtaat
     3541 ttctacacgg cggttttgcg ccttaccttc tgcggtgctg ttgctggcga ttgggttagc
     3601 cgggccaagg ccctgagtac ggatgcggct ggcgtccacg ccctgggtga tcaacgcgct
     3661 ggcaacggaa tccgcacgct gctgggagag acgcatgttc aggtcgtgac cccccgtgct
     3721 gtcggtataa ccaatcacgt taaccgccgt tttcggatac tctgtcagca ccattgccac
     3781 gccggtcagg gtgttagcgc ctgccggttt cagggtcgcg ctgctgctgt cgaaggtcac
     3841 attgttcggc atattgagga taatgttatc cccgctgcgg gttacgctaa caccagtgcc
     3901 gcgcattttg tcacgcagct tcgcttcctg cacatccatg taataaccca cgccgccgcc
     3961 cagagctgcg cctgctgctg cgccaatcag cgcgccttta ccgcgatctt tcttcgaaga
     4021 agagagcgca ccaatacccg cgcccacgag agagcccaga cctgcgccga tagcagattt
     4081 gcctgcttcg cgttcgccgg tgtaagggtt agttgtgcag ccagataccg ccagagcgcc
     4141 actcactacg gcggcaataa gataaacacg tttcttcatt gttaatcctt aataaccttt
     4201 ttattctttg ccacgggttc cgtggcggga gattatgccg cgtgaacatg a
//
LOCUS       DAZPJP010000276         4242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPJP010000276 DAZPJP010000000
VERSION     DAZPJP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.279"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHU9J_004114"
     CDS             complement(130..798)
                     /gene="artM"
                     /locus_tag="ACHU9J_004114"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1907515.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHU9J_004115"
     CDS             complement(798..1514)
                     /gene="artQ"
                     /locus_tag="ACHU9J_004115"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1907516.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHU9J_004116"
     CDS             complement(1521..2252)
                     /gene="artI"
                     /locus_tag="ACHU9J_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1907517.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHU9J_004117"
     CDS             complement(2270..2998)
                     /gene="artP"
                     /locus_tag="ACHU9J_004117"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1907518.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3216..3731)
                     /locus_tag="ACHU9J_004118"
     CDS             complement(3216..3731)
                     /locus_tag="ACHU9J_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1907519.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1086 a         1076 c         1054 g         1026 t
ORIGIN      
        1 tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag gcaccgtgct
       61 gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aacccccccg gcgaatgtac
      121 gcagctacat taattccgcc gttcgaatgc cagtgctttg cgctcgatca gacgcatcat
      181 cagcgtcagc aggccgttga cgaccaggta aataatccct gccgcaccga acaccattac
      241 atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca gcgtaatggt
      301 gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag aagagagcga
      361 gcgtttaaag gcatacggca gcaggagcgc cagcgtatct tttttgctca ttcccagggc
      421 gctacaggac tgccactgac cttctgggat cgcacgaatt gcaccgtaaa acagctgcgt
      481 ggtatacgcc gcactgttca gcgataacgc aatcagcgca cataaccacg gttctgacaa
      541 caaatgccac agtgccggat actcctgcaa cgtcggaaac tggcccggcc cgtaataaat
      601 caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac cccgcaccag
      661 ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg ccagaatcag
      721 tgccacaatc agcgaggcaa cggttagcgt caggctggtg tgtagccctt tcatcagttc
      781 gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg caggtcaatg
      841 cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc cgccacaatg
      901 taccaggtaa atggttcctg ggtacgagta gcgatgcttt ttgtttgcag cattaaatca
      961 ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca ctggttaccg
     1021 aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa gatagccgat
     1081 ttcgacagcc ccagcgcctg accagactcc cactgaccca ccggcaccgc tttcaacgcg
     1141 ccccgaagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat gacaccacaa
     1201 aggaacgggc taacgtcgaa gttctcaatg tccatctgca ctgggatctg cacgaaccca
     1261 agattgatag tgaagccatc cgaaagcgtc agcagcagct gcgaggagcc aaaatagata
     1321 aacagcacca ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc tgaacctgcc
     1381 cacgcgacag gacgccattt tgccgactcc cataccgcaa agaacatcgc cagcgccagc
     1441 ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct tgctaaagga
     1501 aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt ttcgtaagtg
     1561 ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagctc agtgttgccc
     1621 tgacgtaccg cgatgccgag gccagtgccg aagtaatctt tatcggtcac tttgtcgccc
     1681 accgccgcca gtttcgggtt atctttcagc cactcagtga ccactgcggt gtcaccgaag
     1741 acgccgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct gtcatacgga
     1801 acggtagtga tttccgggtg cttatccata atgaatttct ggtgtgtcgt cccgttctgt
     1861 acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg ctgacccaca
     1921 aacagggcag agttgtcata gtacggggtg gtaaacagca cctgcttttc acgctccgga
     1981 gtgatatcca tgcccgccat cacggcttct acgcgacgga atttcaggct tgggatcagg
     2041 ctgtcaaacg cctggttaga gaaagtgcag gttgcatcaa tctctttaca cagcgcttgt
     2101 gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc aaacggagga
     2161 taggatgctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag actaaaacct
     2221 gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct tagtgagaga
     2281 gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg ccttgttcta
     2341 cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc acttcaactt
     2401 cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg atgctgacga
     2461 tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc agtacctgcg
     2521 gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca gaaagatgca
     2581 gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt tctgcacggg
     2641 ccagcgcctg atctttactc aaccccagta cacggcaggg cgcttcaatc aggttttgct
     2701 gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg ttacgacgca
     2761 aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg cctgcaatgt
     2821 tgagcgtacc ggagcgtggc atctcaagca gattgagtac acgcagcagc gagcttttac
     2881 ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc agcgtgatat
     2941 cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga atactcattg
     3001 acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct ttgacagaat
     3061 aattatgcaa tatttctgct ttaaaagtta aaagcaaagc gcattattca ataaacatag
     3121 cacaaaataa cgggggcggt ggtcggcgag cataaatgtc ggcattcctc acgaaatgcc
     3181 ggacaattta cggggtttat tggttgatca aggcgttagc gattctcgat ggactgacgg
     3241 agcgtacccg ccgtggcatg aacgctgccg cctaagtagc gcacatcgtc gatgacccaa
     3301 cactggcctt cctgaatcat taacacttca tcctgccaac cctggtcacc ctgtttgaga
     3361 tctacgcgca atggaatgtt acgggcatca cgattaggga tagtcgatgc actggcaacg
     3421 tgggcgctat ctggcaaggt ggttcgactg gagaacggat cgctgcttaa caattcacga
     3481 tgactattat cgcggctggc atcgctaaga agggttgcca gtttgtcact caaatagggg
     3541 cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcatagaa ttgttgagcc
     3601 acgctgtcag gccccccctg gacacaggaa ccggtgcggg gaccgttatc tttataagct
     3661 ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag agatagtgaa
     3721 cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac gaccaaacgc
     3781 tatcagatta tttaatacat tgtttttaat acaattctga aacaatctga acacctgatt
     3841 gggtttgagt ccattgctgg ctatccactc aaggttgctc gtagctgact tacttaaatt
     3901 acccaaacaa tagacagtgt aaaccatttg tgaggttgag cgtgttatat ggctgaaatg
     3961 atcatttaag gtaaatatga tagttctgaa atggtggtat aaatcctcct gtagaggtta
     4021 agagcatctg caagaagcca gtttcacatt tataacgcgt gataaatgaa cgcggttatt
     4081 gtctaatcat tacaaaattt ttcacatgcg ataagagatc ctgcgattaa gatgattgcc
     4141 aaaattacgt tgtatttttg aaaatgagcg aaatttgata gtcccagctt tgactctcga
     4201 taatattatt tatgctacaa ttgtgggtaa agaatttaag ct
//
LOCUS       DAZPJP010000277         4210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPJP010000277 DAZPJP010000000
VERSION     DAZPJP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.280"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..317
                     /locus_tag="ACHU9J_004119"
     CDS             <1..317
                     /locus_tag="ACHU9J_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907520.1"
                     /translation="FTGVRAMAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQT
                     YTVSVPNMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKW
                     TA"
     gene            319..975
                     /locus_tag="ACHU9J_004120"
     CDS             319..975
                     /locus_tag="ACHU9J_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907521.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
     gene            1038..1310
                     /locus_tag="ACHU9J_004121"
     CDS             1038..1310
                     /locus_tag="ACHU9J_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR1907522.1"
                     /translation="MDIGAILALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAV
                     LESSISRIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(1349..1798)
                     /locus_tag="ACHU9J_004122"
     CDS             complement(1349..1798)
                     /locus_tag="ACHU9J_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR1907523.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIGYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            1835..2269
                     /locus_tag="ACHU9J_004123"
     CDS             1835..2269
                     /locus_tag="ACHU9J_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907524.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            2279..2779
                     /locus_tag="ACHU9J_004124"
     CDS             2279..2779
                     /locus_tag="ACHU9J_004124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907525.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            2855..3166
                     /locus_tag="ACHU9J_004125"
     CDS             2855..3166
                     /locus_tag="ACHU9J_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907526.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            3163..3456
                     /locus_tag="ACHU9J_004126"
     CDS             3163..3456
                     /locus_tag="ACHU9J_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907527.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            3517..>4210
                     /locus_tag="ACHU9J_004127"
     CDS             3517..>4210
                     /locus_tag="ACHU9J_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1907528.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQD"
BASE COUNT         1362 a          704 c          877 g         1267 t
ORIGIN      
        1 gatttacagg agtaagagct atggcacagg gattacaatg ttggaatgca gcgggtgtat
       61 tagtagtaga tctgactgat tataacatac gttttatggg tacttatact gtcaaggcta
      121 cttctgcaca gacctatact gtatcagtac caaatatgaa aactacaggc tggtttgtac
      181 actatgcccc cgccagtgat acctttaatg agtggtcagc cttttgtaat aatgggtcat
      241 ttactgcggt ttatttgcca acatatgcac cgttagctgg taactacact tttaacgtat
      301 ataagtggac ggcgtaagat ggcaggtttc gaggtatata attcagcggg tgcactgact
      361 atagatagta ctaacaagtc gataatgacg ggtgcagtaa aggccatggg aaatctaact
      421 gatacgggat actacacagg ctttacctgt gccttcggta atggtggatc gctcggtttc
      481 gttatgccat cagtagtagc taacaggaac acaacacagt actggttcca gatacagaaa
      541 gacggggcat ggtgtttccc tggtgcctat atgttccagc ctggcatggg caggttcatg
      601 acaagtagtc acacggctac ccccacatct ggttttcttg atgtgttcag tgaagaaggt
      661 actttaatct ggtctgctgc atcagcggca acaatgccac gtatcagggg atttttgaca
      721 gcacctgcgg ctaccgatct cagtactgcc attacggtaa cgtctccagt agctgatccc
      781 tggttctgct ggtcacaatg tccggggaac atatcagatg atggtacagt aattgggtac
      841 tctggtctgg ttatcaggcg taattccagt acttcattca gtctacagta cgtttcaaag
      901 aaccagaaaa cctaccgcca ggcaatgggg aataatggta tccagatagc attggcgaca
      961 tttacagggt attaatacaa aaacaataac aataacaata acaataacaa taacaataac
     1021 aacaaaggga tttcagtatg gatatcggtg ctattttagc tcttgttatt tctggatgcg
     1081 tattccttta tactatcttt agagataata caaaggatac gaatgatcta ttaactcgcg
     1141 ttgctactct tgaaactacc attgctgtac tggaaagtag tatttcccgt attgaggatg
     1201 atcaggataa gatgagagat acactaagta aactggaaac acaaatccat gaacttgatg
     1261 ttaagattga aaggattatt gcaattcttc agcagaaaca acgaccataa aaaaaggcat
     1321 agtatgtcat tcgtactatg ccttttcttt atttgttttt aagtactgca atcatggaat
     1381 tcacacggtt tggtgtctgc ctgtaccata gagaacactt agcctgttta attgcttcaa
     1441 ggtaatcatg tgctttcagg gctgctaaca tcttacggaa tttcttcacc ccgccaatac
     1501 ctaactggaa ggtcatgata ataataaaat cattccagtc atcgggaaga tttaacccta
     1561 atgtctgtac ctgcataatt gttttggcta aatcgcgaga tagtaacttg tctgcttcgc
     1621 tttcagtaat gccagtacta aaatcttctc ccgcctgaat taagtgacca tatccaatag
     1681 tactacatcc taaactatca gcatatggat agaatttccc atttctgaag tacttcaaat
     1741 ttgcctgata ctctttagta ccttcatatt cttttaatcg tgtttttaaa tcgctcattt
     1801 actaaatacc tataatgaat ttataggaat atttatgatg agtgaatgga aatactcgga
     1861 tgattggagc gaggaggaaa tgttatccgg tgaatatgct ggtttcgttt atatctttca
     1921 gtttgaggat ggttctaatt atgtcggtgc taagcaaatc tatcagcggg ttaaggatgc
     1981 gagaaagcta aagcccactt caaaggaaaa tggctggcgt gagtacctca gtagttccaa
     2041 tgtcgttaaa aacaaaattg aaaatggtga acagtactca aaaacgattc tatggtgctt
     2101 cgcaactatg cgagaggtaa tgattgtaga ggcaatactg atccttcatc agatgcttaa
     2161 accggaatgt ttgaatctgg caatgatgag taagattaga tcaccaaatg caaaggataa
     2221 aaaaagacta ctcggaatag tacaggaact attggagtac ttgaattgag gtaaatatat
     2281 ggcaagtgga ataaatggga ttggtagtat aaggaactat atacaaacgc agggagttag
     2341 attaaataat caattccaga atgaaataat taaacgttcc aagctactat cccaaagaat
     2401 gcagggtgat ttgaataata gcgttgaccg tggggcagtg ggctttactc aacgttccat
     2461 ccttttcttc tatagaaagg ttgggagaaa ttcagttaca tcgaccatca tggttaaaga
     2521 tattcaggct aagtaccttt atcaagtact ggtacagcca aaggcaattg ataagtttat
     2581 acctacttca tctacaagat taactaagca gggcaacatt tcaggactga aaaagaatct
     2641 tttaagtgaa cgatataagg tagtgaaggg taagaatggt aaagaacgat taattgatac
     2701 cgcaaagaaa gataccaaga agaaaaccaa acgtgtaatt ggcttacgtg agaagaaaca
     2761 acgtcgcctt atctattgac ttctataagg aagccaatga tggtgtaagg gtaattatga
     2821 gcggtattca gggaacattc aggataagta gacaatgaat tttgagcaac attatggtga
     2881 tttaataagc aagattaagc taaatggcat gacaccagat ggtaatagta tgccaattaa
     2941 caaatgtttt cttgataaga aatttcaaaa ggtcgttaag aaaagtaatt atcagattga
     3001 tagctatatg aacgttattt atgagaagaa gaaagatttc aaagttggtg aagtacttga
     3061 atgggaatta cagggtgaaa agatgcctgt atttctaatt gagagtaaaa agctctttgt
     3121 gaagggtaaa catttttggg tatatgccgt ggggattatt gaatgattag tatattgatt
     3181 gagtttctga agtcaggatt ggattttttc attaagaaga aaactattga acaggaagta
     3241 aagaaaacga atgtagaagg gcagattgaa acgaataaag aagagattga aaaggtaaca
     3301 ttccactggc gtaatgccct cggttttgta ctaaccctta tcattctcta taactggatc
     3361 atagtaccag tacttgatgc ttttggaatt attgtgatcc aagttccatt aggacaactt
     3421 ttgcaagtgc tattgattat ggttggtgga agctgaaaga tttttccttt aatatgtgtt
     3481 ttgagttaat aaaatagcaa tctaaggatt tgatttatga gtgatgcaat tactacagca
     3541 gcaataaaaa aattgtatgg actatccgct gggcaatgta atatttgccg aacttctctt
     3601 tttaaagagc aggttcacat cggacaaatg gctcatgtaa tagccaaaag cccaaaagga
     3661 ccaaggggcg atgacctgtt agcaaatgac aattcttatg aaaatcttat cctgctttgt
     3721 gcaaatgatc atattcttgt tgatagagat ccattcactt atacagtaga aaaattgcat
     3781 caaatcaaaa gtgaacatga ggcttatata cagggtgcaa ccgataatac attattttca
     3841 gataaaaaaa gacagtcaga tgtggacttt ttaaacgctt acttccactt tacaccgttt
     3901 gcgagggtac gttcattagt tgaaacttta cctaattctt ttcacatcaa tcttcttatt
     3961 tttggagatc agtttgatag tattttaagg gattttccag cgagctatcc tttaaatgat
     4021 aaattactcc acggtcattt ccaaagtttt attgattcgt ataaaaatat tgattttatc
     4081 atatctggac atttgccgat ttccgataat agaaacattc aggtgtttgg tggtgcgaat
     4141 cataatcttc tttgtcattt caattatgat gagctaccat ccgataaagc actgataata
     4201 gaacaagatt
//
LOCUS       DAZPJP010000278         4087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPJP010000278 DAZPJP010000000
VERSION     DAZPJP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.281"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="ACHU9J_004128"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="ACHU9J_004128"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1907529.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="ACHU9J_004129"
     CDS             930..1382
                     /locus_tag="ACHU9J_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1907530.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="ACHU9J_004130"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="ACHU9J_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHU9J_004131"
     CDS             complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHU9J_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1907531.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHU9J_004132"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHU9J_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR1907532.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          899 a          906 c         1077 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAZPJP010000279         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPJP010000279 DAZPJP010000000
VERSION     DAZPJP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.282"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHU9J_004133"
     CDS             <1..784
                     /locus_tag="ACHU9J_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1907533.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHU9J_004134"
     CDS             797..1756
                     /locus_tag="ACHU9J_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1907534.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHU9J_004135"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHU9J_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1907535.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHU9J_004136"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHU9J_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1907536.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZPJP010000280         4056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPJP010000280 DAZPJP010000000
VERSION     DAZPJP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.283"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(109..1428)
                     /locus_tag="ACHU9J_004137"
     CDS             complement(109..1428)
                     /locus_tag="ACHU9J_004137"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1907537.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1559..3094)
                     /gene="gadC"
                     /locus_tag="ACHU9J_004138"
     CDS             complement(1559..3094)
                     /gene="gadC"
                     /locus_tag="ACHU9J_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1907538.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3250..>4056)
                     /locus_tag="ACHU9J_004139"
     CDS             complement(3250..>4056)
                     /locus_tag="ACHU9J_004139"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1907539.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1039 a         1021 c         1039 g          957 t
ORIGIN      
        1 tgcgtggtta atttgactta tgtaagcatg attactagtg ggatatttca caaggtgtaa
       61 caagccggtg ggtaaagcac cggcttgtta caaagtaagc atgggagttt aactgcccca
      121 acgactttgc agatagctga ccgcttgttg agtctgcggt ttattcagat agtcctcacg
      181 gaacaagatg gtgccgctaa tttctggtac cgcatcgtta agatcgagct gctttttcag
      241 ttccggtacg ccgccgttaa tcatccagtc tggctctatc tttgaaggtt cacccacttt
      301 atagaaggcg ataccgatat acaggcgggt cctggtcggt ttaacgacat ccgcccacca
      361 ttttgccaac acgtcataac gcgcggcact ccgtgagaac ggccagtaaa tttggggagc
      421 aatgtaatcc agcaatcctt gttccaccca tcgacgggtg tcagcgtagg attcgtcata
      481 ggctgccgcg cctcgggtat cggaaccgag cggatcgtgt gatcggttac gccacacgcc
      541 tgccgggcta acgccaaatt cgactcccgg cttaatgctt ttaatggtgt gcgatacttt
      601 tgcaattaac tgctgagtat tgttgcgccg ccagtctgct tttgacgcaa atgcgcctcc
      661 gtatttgcgg tacgtttcgt tatcatttag ccgtgaaccc ggtgactcag tatagaaata
      721 gtcgtcaaac tgcacgccat ctaccggata gcgggaaacc acttctgcga ctattgatgt
      781 gatccagtcc tgaacctcag ggatgcccgg gtcgaggaca aagcgatcgc cagacgttct
      841 gatccagtca cggtgttgca catagacgct cgccggttgt tgagacagag tgctattcag
      901 ttccctgata gtaccgggct tcgtattaac cgatacgcga taggggttaa accaggcgtg
      961 tactttcatc ccacgcttgt gggcttcgtc gagcataaat tgcagcggat cgtaacccgg
     1021 attttcacca atcttaccgg tcataagatc ggaccatggc aaaattttcg atggccacag
     1081 ggcggtaccg tccggcttaa cctggaaaaa gaccgtgttt ataccgagac gttgcagatg
     1141 atccagtttg tcgatcatcg cctgttgttg tacacgggcc cggctggtgg ggttactaat
     1201 gttaaccgag gaaaccggtg gccagtcgag gcgagacacc gtggccagcc agatgccacg
     1261 catcggttgt gacgattgtt gcgtcgtggc tggtggcttt gaacctgctg gtggtgtcac
     1321 catggactct ggaggcgtgc ttttacaact acacagtaaa agtgccagtg caaccagtat
     1381 cgctggtctt ctaatcgcta atttctcgtt tcgggagcag atatccatat atgctggttt
     1441 ccggtaaaca gatgtgcgct cattctcgta cttatcccgg ttaagtcaat acgacagcga
     1501 gcacgaaaaa gggagcgatg aattatcgct cccttgtctt ataaccattc agacatggtt
     1561 agtgtttctt gtcattcatc acaatatagt gtggtgaacg tgcacgcggg tgcaggaaga
     1621 agtgaccttt tggtgcgttc tgactgttga ttggctccag agtgacgccg gtattcgctt
     1681 tgcctttacg atcatgaaca gcatagagaa taaagggcag ggcaagtacc accaggaaac
     1741 taacgaccag taattcaaca tacatatcgg tagagtcacc ctggatgtta tccggcggca
     1801 ggaaggaaac aataaacgcc ataattgaag tcagcagacc gacaatagcc acgaccagtt
     1861 tcaccccttt accaccaggg atattaaatg tgcgttttaa gtcaggatgt ttaagaacca
     1921 acacaatgta gccaataaac agcatgaaat aagcacacag ataaatcacc accgtcagcg
     1981 ccagtgcgat caggaaggac atgttgttac cgccaccggt attggtgagg atgatcaacg
     2041 cgatagaagt aatcaccagc tgtgaaatga ccagcgttac cggtacgcca tttttgttca
     2101 ttttagcgaa tgccgctggc agcaggtttt tctgcgctgt gacatacatc ccgcgagaag
     2161 gaccaacaat ccaggaggcg atttccgcca gaacacccag caacagcagt gcggagatca
     2221 cgcgaaccgt ccactcaatt tccggtgcca catgggacat cagaacggta aaggtttgca
     2281 ttacccctgc ggagaggttg atttcattac ccggaatgac catcgcaata gacaaaccac
     2341 caacagagct taagcagatt gccgctacca tcagcagtaa catagccaac ggatagtcgc
     2401 gccctgggtt gctcatttca ttgacgtggg ttgcggatgc ttctacgccc atataactca
     2461 aaatgaaggc aacaaataca accagggtgc ccactttaga gaagtcaggg aagaaggtct
     2521 ttgaatccat ttcgatagca acgggggcac cggagtgcag ataaatagcc gctaatgcga
     2581 tcaaaataaa tgcaggtaac aggataccgg cgaagaagcc aactttagca attcgtgccg
     2641 tgtatttcgt gccaccaaac tgcgttaatg ccagcgccca aagaatgatg agtgctgcaa
     2701 tagttttggt aatggggtct tcattcagcg ctggccattt caggatgtag gagagtgccc
     2761 ctaacacgaa atagagcatc ggaataaaac caatggcgat ttgcagatag ccaaatgaga
     2821 tcgctgcaaa tccccatctc ggccccagag tatttgatac ccaggcgaag acaccacctt
     2881 cttcccagcc gtcgacggtg gccatttccg cagcacaaag tcccacggga ataaaccata
     2941 aaatcccgcc taatagcagg aagaagacta atgaaaagcc cgatgttgcg aaggtagggt
     3001 attcataaac agccattacc atcgatgccg ttatggcaaa aaagccgagt aatgtgagct
     3061 gcttagcttt acctgtctgt actgatgtag ccatattatc cccctaaaac ggtattcctg
     3121 tcggaaccgc acctgttttg ttctgaagcg tattcagaac aatattttcc gttgctaatg
     3181 ccagtgaaca gactttggaa attatcccga aacgggttcg tttcgggaca ccgttaccgt
     3241 taaacgttat caggtatgtt taaagctgtt ctgctgggca ataccctgca gtttcgggtg
     3301 atcgctgaga tatttcaggg aggctttgta gtcttccagc aacagttcag caaagtccat
     3361 ttcgaagccg cgacgacaca taatgcgcat caccacgatg tcggtggctt caccgccgag
     3421 agtgaaggcc ggaacctgcc agccgcgcag acgcagacgt tcagagaggt cgtacagggt
     3481 gtatcccgga tcttcaccct ctttcagttt gaagcaaacc gccgggatgc cttcgtccgg
     3541 gcgaccggca cagatgaact catacggccc cagtttggcg atctcatccg ccagataggc
     3601 agcgacctgg taggaagcat tctgtacttt ggtatagcct tcacgaccaa ggcgcaggaa
     3661 ttcatagtac tgtgcaatca cctgacccgc cgggcgggag aagttgatgg caaaagtacc
     3721 aatctgacca ccgaggtagt caacgttgaa caccagttcc tgcggcagcg cttcttcgtc
     3781 acgccagata acccagccgc agcccagcgg agccagaccg aatttgtggc ctgaagcact
     3841 gatcgatttc acacgcggca ggcggaagtc ccagacgata tccggggcga cgaacggtgc
     3901 caggaagcca ccgctggcgg cgtcgatgtg catgtcgatg tcgataccgg tgtcggcctg
     3961 gaatttatcc agtgcatcgt gcagcggttg cgggaactca tagttaccgg tgtaggtcac
     4021 gccgaaagtc ggcaccacgc cgatggtatt ttcgtc
//
LOCUS       DAZPJP010000281         4051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPJP010000281 DAZPJP010000000
VERSION     DAZPJP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.284"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(215..862)
                     /gene="ychE"
                     /locus_tag="ACHU9J_004140"
     CDS             complement(215..862)
                     /gene="ychE"
                     /locus_tag="ACHU9J_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1907540.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1340..4015
                     /gene="adhE"
                     /locus_tag="ACHU9J_004141"
                     /gene_synonym="adhC"
     CDS             1340..4015
                     /gene="adhE"
                     /locus_tag="ACHU9J_004141"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1907541.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1123 a          998 c          941 g          989 t
ORIGIN      
        1 atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat aacatgatgg
       61 tgaggagaag cagttaagga aatccttata actaactgta atttgagaag aatattttta
      121 cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg acgaagtaaa
      181 cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg gggaaaatcc
      241 ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc cccataatac
      301 gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt gccatgcgga
      361 acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca aacagatagc
      421 taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct ggccccgcca
      481 tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt tctgattttt
      541 cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc gctatcgtca
      601 ccaccaggat gccccctgcg atacggaacg aatcaattga tataccaaaa agttgcagaa
      661 tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg ttggctgtaa
      721 ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata aagacgggaa
      781 taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag taaacgggaa
      841 aatccaaaaa ggtctgaatc acggttagct ccgaagcaaa agccggataa tgttagccat
      901 aaattaaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
      961 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
     1021 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
     1081 aaggtgtcag cttagcaaaa atttgatttg gatcacgtaa tcagtaccca gaagtgagta
     1141 atcttgctta cgccacctag aagtgacgca ttagagataa taactctaat gtttaaactc
     1201 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat aggttaagca
     1261 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
     1321 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
     1381 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
     1441 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
     1501 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
     1561 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
     1621 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
     1681 taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat
     1741 tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg atatcgttct
     1801 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
     1861 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
     1921 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
     1981 gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
     2041 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
     2101 tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg gctatctgtt
     2161 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc
     2221 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
     2281 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
     2341 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
     2401 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
     2461 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat
     2521 cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
     2581 accttccctg actctgggtt gtggttcctg ggggggtaac tccatctctg aaaacgttgg
     2641 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
     2701 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
     2761 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
     2821 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
     2881 cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
     2941 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
     3001 tatgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
     3061 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
     3121 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
     3181 cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata tggcgattgt
     3241 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
     3301 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
     3361 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
     3421 taaaaatccg gtagcgcgtg aacgtgttca cagtgcagca actatcgcgg gtatcgcatt
     3481 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
     3541 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
     3601 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
     3661 ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa
     3721 gatcgagaaa ctgctggcat ggctggaaac gctgaaagct gaactgggta ttccgaaatc
     3781 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
     3841 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
     3901 gaaacagatc ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
     3961 gaaaaaagaa gccgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
     4021 agcgctgtct ggcaatataa acggcccctt c
//
LOCUS       DAZPJP010000282         4047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPJP010000282 DAZPJP010000000
VERSION     DAZPJP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.285"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            29..757
                     /gene="mpaA"
                     /locus_tag="ACHU9J_004142"
     CDS             29..757
                     /gene="mpaA"
                     /locus_tag="ACHU9J_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1907542.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(732..1697)
                     /gene="ycjG"
                     /locus_tag="ACHU9J_004143"
     CDS             complement(732..1697)
                     /gene="ycjG"
                     /locus_tag="ACHU9J_004143"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1907543.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1816..2322
                     /gene="tpx"
                     /locus_tag="ACHU9J_004144"
     CDS             1816..2322
                     /gene="tpx"
                     /locus_tag="ACHU9J_004144"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1907544.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2366..3907)
                     /gene="tyrR"
                     /locus_tag="ACHU9J_004145"
     CDS             complement(2366..3907)
                     /gene="tyrR"
                     /locus_tag="ACHU9J_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1907545.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1141 c          972 g          964 t
ORIGIN      
        1 tgtaatactt cctgaaccag agacgatcat gaccgtaacc cgcccacgcg ccgaacgcgg
       61 cgcatttccg cccggaacag aacattacgg acgttcatta ttgggcgcgc cgttaatctg
      121 gtttcctgcc cctgcagcca gccgcgaaag tggtttgatt ctggctggca ctcacggtga
      181 tgaaaactct tcggtcgtca cgctctcctg tgctctgcgg acgttgacac cttctttgcg
      241 ccgccatcat gtggtgctgt gtgtgaatcc tgacggctgc cagctggggt tacgggccaa
      301 tgctaatggt gtggatttaa acagaaactt tccggcggcg aactggaagg aaggtgaaac
      361 ggtttatcgc tggaacagcg ccgctgaaga acgtgatgtc gtattactga caggtgacaa
      421 acctggctca gaacctgaaa cccaggcgct gtgccagctt atacaccgca ttcaacctgc
      481 ctgggtggtc tcattccacg atccactggc ctgtattgaa gatcccagac acagcgaatt
      541 aggcgaatgg ctggcccagg cgtttgaatt gcctctggta accagcgtcg gttatgaaac
      601 gcctggctct ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca ccgcagagtt
      661 tccgcccatc tcctccgacg aagccagcga aaaatatctc tttgcgatgg caaacttgct
      721 gcgctggcat cctaaagatg caattcgccc gtcgtgaact gaagcgccgg ttccacatct
      781 accgccagcc aggtcggtcc gtcaagatcg gcgaaactga cctgcggcac cagcggtaaa
      841 gcggcgctaa tggcacgaga ggtacacaac atgcagccca gcatcagact gaaaccttgt
      901 gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccgatttt atcgagctta
      961 atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgtgt atgacaactc
     1021 tcatcagcac aaatcggcaa cggatgaata aaattctcca gcgccgcatc gtcctgcgca
     1081 ggaaggggtt gttcaagcat cgcaacgcct aaatccgcca atagctggca acgcgccgcc
     1141 aacccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc gggcacagct
     1201 gtgcgaattg ccaccatccg ctcactaata agatggttat ccagcttcac tttcagtaat
     1261 ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg cgtaccgatg
     1321 actaccgtct gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa atcagccagc
     1381 gattgctgct gttttcgcgc ggccagatcc cacaatgcac aatccagcgc attacgtgct
     1441 gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca gccctttctc taattgcgac
     1501 acgacgctca taatttgcgc cattaccgag gcatcacttt ctccataacg cggatacggc
     1561 gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac cacgcgcgct
     1621 tcactgcgac ttccccgggc aatcacaaac ggggtatgta agggccaggc ttcctcaaat
     1681 accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa
     1741 tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa ctatatgtaa
     1801 acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt tacagtcgcc
     1861 aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtggc aaaagatctg
     1921 tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt
     1981 attgataccg atgtttgcgc cgcatcagta cgcaagttta accaactggc aactgagatc
     2041 gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc
     2101 ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc tgaatttctg
     2161 caagcttacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt
     2221 gtggttattg acgaaaatga caatgtgatt ttcagccagc tggtggatga aatcaccacc
     2281 gagccggatt acgaagcagc tctggctgta ctgaaagcat aagttaatat gcctgatggt
     2341 gttgcaccat caggcatatt cgcgcttact cttcgttctt cttctgactc agaccatatt
     2401 cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg
     2461 tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg ctggtgattt
     2521 cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg tcataatccg
     2581 gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga
     2641 tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt acagtattca
     2701 ggtcagcggc cagtttcgga cgctgcacgc cctgctcgtc ggcaaagcgg gcgacgaaca
     2761 gctcagttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga ttgagcgtca
     2821 acacgttcag acgataatag agatcttcac ggaacatgcc tttttgcacc agttcgacca
     2881 gattcttctg cgtaacgcaa atcacccgca catcgacatg cacctcatgg tcttcgccaa
     2941 cccgacggaa agtgccatca ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca
     3001 tttcccctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag aatcctttct
     3061 tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt atagacgcac
     3121 agttcagcgc caggtaaggt ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa
     3181 agagatcttt acctgtacct gtgtcacccg taatcagcag cggcgcgctt agcatcgcca
     3241 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacggcgaca atttgactga
     3301 aggcgctgac gtcctgggcg gcgacatttt gcaactggcg gcccatacga atcgttgatc
     3361 gcaacatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     3421 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt
     3481 gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat
     3541 ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat
     3601 ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc
     3661 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     3721 ccgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc agttcagcaa
     3781 aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca atgcctctta
     3841 gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca
     3901 gacgcatggg aaccttcacc tgaaaaaaga acaattaata tgatgatatg acactatttg
     3961 gatagcagga aggtttctgt caacaatcat tgacacaaag acggaaaaac gggcacttcg
     4021 gcgtaaagat atccggtcag cacccga
//
LOCUS       DAZPJP010000283         4022 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPJP010000283 DAZPJP010000000
VERSION     DAZPJP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.286"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..366)
                     /locus_tag="ACHU9J_004146"
     CDS             complement(<1..366)
                     /locus_tag="ACHU9J_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1907546.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSE"
     gene            complement(381..1127)
                     /locus_tag="ACHU9J_004147"
     CDS             complement(381..1127)
                     /locus_tag="ACHU9J_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1907547.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1134..1442)
                     /locus_tag="ACHU9J_004148"
                     /pseudo
     CDS             complement(1134..1442)
                     /locus_tag="ACHU9J_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1821..2333)
                     /locus_tag="ACHU9J_004149"
                     /pseudo
     CDS             complement(1821..2333)
                     /locus_tag="ACHU9J_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2346..2768)
                     /locus_tag="ACHU9J_004150"
     CDS             complement(2346..2768)
                     /locus_tag="ACHU9J_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1907548.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2765..3007)
                     /locus_tag="ACHU9J_004151"
     CDS             complement(2765..3007)
                     /locus_tag="ACHU9J_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1907549.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3104..3523
                     /locus_tag="ACHU9J_004152"
     CDS             3104..3523
                     /locus_tag="ACHU9J_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1907550.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3829..3984
                     /locus_tag="ACHU9J_004153"
     CDS             3829..3984
                     /locus_tag="ACHU9J_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1907551.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1008 a         1080 c          893 g         1041 t
ORIGIN      
        1 ttcgctcttc cggcactcgc gacagattat attcaggcgc ctgtcgtagc ggcgtatttc
       61 tccgtctggt aatgaccaga taaggtcagg atcaaccaca accggtttct tcacctttac
      121 cctcgatagc tttttgcggg cgttttgcca gtctttatga gcctgttcag agggaaatac
      181 cccgtagcca gaattatata catcaccact tgcgaccagt tccatgcata agcgaccgac
      241 agacgcacga ctgatacctg tttcatctga caactgccga atcgtgaccc gaccgtcctg
      301 atgcacgagt tccacaattc gcgtcttcag ttcttcccgc tgttcgggag taaaaggttt
      361 cgccataaat cctcctgaaa ctacttaaca accctcgaat ggctaacatt cggacgccag
      421 ctctcccagt taaaattcac ccagcgcccg ccgttcatga ccatgcgatc catcacccgc
      481 tcgccgagaa gtgtattcat cgctgcatgg ttaatatttg tcagcatccc cacaccgcgt
      541 aacgatgccg tccggcgatc aacaatctgg ttcagtacca cctgctcgtt tttcgtatcc
      601 cgctgcatgc caatttcatc caggaccagc aggtcaacac cacaaagctc ctgtaaaaat
      661 ttttcaccgg atttgccgtt gtcgtagctc tcatgcaaca cgctcatgac atcggacacg
      721 gtgacgataa tcacgctacg ccccttcgcc atcagccggt tgccaatcgc gtctgcaaga
      781 tgattttttc cggtgccggg cttaccgctg aacacaaaat tcgtacagcc tgtcatcagt
      841 tcgtcagcta tggatttcgc ctgactcagt gcatgtcgct gaccgtcgtt ctgcgcccgg
      901 taattcgcaa acgaacactt ccggtgcaac ggctggatgc cggagcggtt caggattttt
      961 tccacccgca actgacgatt caggcggttg atctcctcgc aacgtttctg gccttcagca
     1021 agttgccact cgcgccactc cgcaaccgtt ctgaatgggg cggttacatg tggtggggtc
     1081 agtctgcgga tacgctccag aacgcctcct gtcgcaatat ttttcatggt ctgttacccc
     1141 ctgaagcctg gcgggatcgc actgtccggc aacgagacgg tgttaacctg tcggagcaac
     1201 gtctcaggcc gaacaccttt cggcgcgaac agaccctggt attcattggc gatgctgtgt
     1261 cgaatcacct gctcaggtgt aaaaccctgc tgacggaatt tttccagttc ccgtatcgcc
     1321 ccgttagcgc cctgctccgt tcgaatcggt tttcgcaatg cctgcctgaa ctggacccac
     1381 tcatgccaga gtgtttctgg caaccagtcg ggcagatcaa ctgacaacgg gtcaaatttt
     1441 ttctggcgaa cagccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca
     1501 ctgcgccgca agacacatat cccggtttca acagcaccga ttttgagcag tgatttctgt
     1561 cgcggagaat ttcggtcaag ctcgctccca caccggacac cgttcctgtc agtcacgacg
     1621 atatcaatcc gccctccgca accgtcacca cgctctggca ccggatactc acgacagaca
     1681 tccaggccag cagcctgcaa cgcaaccacc gcagaattac aaaattccgc tgcagtaccg
     1741 ccagataatc tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg
     1801 aagggtttgg gatgggttag atctgttttt agatctttat ctgtatcttt attagttgcc
     1861 tttgtgttgg catcatgttc gaacatcact ccaacatctg tttgaacacc tgttaaattt
     1921 ctctcttgtt ttgtttgaac atctgcttcc tttctgcttc ttctggcctg aacagatgct
     1981 tttcctgcgg ctgatttttt ggttaatttt tctctgacag atgccagatc ttcctcaatc
     2041 cgaagatgca tccattcgtc gccgttatca caaaaaaact cccgcaagga tggttcaaca
     2101 tcaacccatc gctcgttagt cagacgggca atttttgcca acctgttttt gggtattggc
     2161 tttcctgttt gccagtaatt gaacatcagc aacaaatacg cgccgtgctc ctctgctgac
     2221 aaatgcatgg tgtcagccag gtaatcagct atgtacagtt gcatgtatgg taatgcggcc
     2281 ataattgccc cgtatgatgc tgcccggttg cttagaataa gcacaaacag catggaaact
     2341 tttgcttaat gaacaatgac agaatcgtcg gaagacccgc cgccgctgaa atgcgctttc
     2401 cggtaaacgg cctggactgc atcatcatgc gcatcaattg ccgtactcaa cgcttcctgc
     2461 gccgccagta atgcacggcg ttccagggta tcgaagatgc agagtcggtg acgcagctcg
     2521 cgcggaagaa ttgccagaac cgcagggatc agtttctgaa ttttttccct ttgcgctttc
     2581 gtttcacctt tcaaccaacg gtgatagata ttctgctgat tgttccagtc cttgcctggt
     2641 acaaggggca attcgccgcc cccctggcgc agatattctt cagtaattgc gttagcgacc
     2701 cacgcctgcc ctttttcggc tgccagggct aacaacactg attcgatgtg ctcatgcctg
     2761 attttcatga atcaaccgct cctatgctgt tttcgctatg cttaccgtct ggggggaata
     2821 catcgtcaag tccacaatga gcgccaagcc gattaagggt agaaacaatt tttctgcact
     2881 cctctagtcc aggggtacga aaatttgctt cgtaatttgc cagtcggctt tgtatccacc
     2941 ctaactgaac agcaagttgt ctttgagaca gcccaagctg ttttcgatat gttgaaattt
     3001 tgttcattga aaacctccga tgacaatttt aaacacacct tgtgttatat agccaagctg
     3061 ttttgtgttt tatgtaaatc acgattcgtg atacaaggat gcaatggaaa aagaaaacga
     3121 aaaaattgcc gctagtaggc tcaatgacaa aattgcaatg cgtcttaaag agcgcaggca
     3181 gaagcttggt ttatctcaag gaaaacttgc tgaaatctgc ggatggacgc aatcgcgtat
     3241 aggtaactat gaggcgggca gcagaaatgt tggagtacat gacgctgtcg tattgggaaa
     3301 ggcacttggc atatctcctc ctgagctcct ctttggagaa caggaatctt ctgaattgtg
     3361 gttaaatgaa tcccaacgaa aacttcttga gttgtttaac cagctaccgg gctcagaaca
     3421 acaacgaatg attgagctat ttgaagtccg gctaaaagaa atcgatgagt atgtagaaaa
     3481 atatttgaga ggcaggatta aagataatcc cccaccggaa taatgatctt gctatcacag
     3541 taatatgcca atcagcccgc tatcagcggg cttttttgta gcatcatcat atgacactca
     3601 ccataaaaca cattgcgtgt taacacaaga aaacaaattg tgtttaataa gcatatccaa
     3661 acaacgcccc accagagaac ggcaggacaa tacctcgagt tatccagtca ctgaacaggg
     3721 ctaagtagcc agcctgaggc atacgaacat gacggcagtt gttgattgat acaaagcgca
     3781 gtagataaaa cgttccgcca cccggcgtta aggggaaatg gggtcaacat gaatactatc
     3841 gatcttggca acaacgaatc tctggtatgt ggcgtgtttc ccaatcaaga cggcacattc
     3901 accgccatga cgtataccaa aagcaaaacg tttaaaaccg aagctggcgc acgtcgttgg
     3961 ttgggaagac attcaggtga gtaaaatgaa cgagacagaa ttaaaacacg ttatcgctct
     4021 ac
//
LOCUS       DAZPJP010000284         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPJP010000284 DAZPJP010000000
VERSION     DAZPJP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.287"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHU9J_004154"
     CDS             30..968
                     /locus_tag="ACHU9J_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1907552.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHU9J_004155"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHU9J_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHU9J_004156"
     CDS             2310..2603
                     /locus_tag="ACHU9J_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1907553.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHU9J_004157"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHU9J_004157"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPJP010000285         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPJP010000285 DAZPJP010000000
VERSION     DAZPJP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.288"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1364
                     /locus_tag="ACHU9J_004158"
     CDS             <1..1364
                     /locus_tag="ACHU9J_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1907554.1"
                     /translation="SLFSASSGINWRDNLYDASFRGVPFSVESDEGSFGRRVQVHEYP
                     NRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLIHPQFGEMQGC
                     IDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVFLDELIEDMFG
                     DFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDLSVILMPPSVA
                     SDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPTDSASVIRQKQ
                     QTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQSDIIHVSHPAL
                     DSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQLTSLRTELNRD
                     IQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHPGFVPVRALRV
                     PVR"
     gene            1361..2437
                     /locus_tag="ACHU9J_004159"
     CDS             1361..2437
                     /locus_tag="ACHU9J_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1907555.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            2415..2948
                     /locus_tag="ACHU9J_004160"
     CDS             2415..2948
                     /locus_tag="ACHU9J_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1907556.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            2954..3364
                     /locus_tag="ACHU9J_004161"
     CDS             2954..3364
                     /locus_tag="ACHU9J_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1907557.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            3357..>3946
                     /locus_tag="ACHU9J_004162"
     CDS             3357..>3946
                     /locus_tag="ACHU9J_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1907558.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
BASE COUNT          884 a          969 c         1182 g          911 t
ORIGIN      
        1 cttcactctt ttctgcatct tcaggtatca attggcgcga taatctttac gacgcttcat
       61 ttcgtggcgt tccgttttcg gtggagagcg acgagggttc gttcggacgc cgcgttcagg
      121 tccatgaata cccaaaccgt gacaaaccgt acacggaaga tctcgggcgt gccacgcgac
      181 ggctgacgat taatgcgtat ctcgttggtg atgattacgc agagcagcgc gacaggctga
      241 ttacagcgat tgaaaccgcc gggccgggga cgctgatcca tccgcagttc ggtgaaatgc
      301 agggctgtat tgacgggcag gtgaccgttt ctcactccgg cactgaaggc cgtatgtgcc
      361 gggtttcatt tcagtttgtt gagagcgggg aactgtcatt tccggtcgcc ggagctgcaa
      421 ccgccagaaa actggaggag tcgtccgtat tcctggatga gttgattgaa gacatgttcg
      481 gcgattttga tctcgcggga ataccggact ttattcagaa cgatgttatt gcccggacga
      541 ccgatatgct gggaaccgtt cagacagctt tcaaaatggt taattcggct gtttctgccg
      601 gagcgagact actgcagggc gatttatccg tcattctgat gccgccgtcg gttgccagtg
      661 attttgtgca tacgttgcag gatacctggc gggccggaac ccggctggtg gataacacac
      721 aggatctggt gcagtccata acgacaatgt ccggcattac gctggaccca ggactggcac
      781 cgcgtgcggt gtggcccaca gattccgcat cggttatcag gcaaaaacag cagacaaacc
      841 ttgtggctgc tgtcatccgg acgacggcaa tcagcgaagc cacaagggcg gtcacttcac
      901 tgccacaacc cggaagcctg gtgaaaaatc agcaggcggt tgtggctgtt ggtggttcca
      961 ctgaacgtca gtccgatatt attcacgttt ctcatccggc acttgacagc gtggcagcca
     1021 gcacaggaca ggatgagaga gcgcaaccac ccacgcggga aaatctcacc atcatacgcg
     1081 aatcgctgaa cgcggcgatt gaacaggagc tcaggcgtac ggcggatgac aggctgtttt
     1141 ttcagctgac gtcattacgt acagaactga accgggatat tcaggcgcgt ctggttcaga
     1201 cggaggaaac cgcagagcga acgccagcgg aagttctgcc tgcgcttgtt ctggctgcgt
     1261 catggtacga cgatgcgtcc cgtgaaactg atatcctgga tcgaaatgcc atctctcatc
     1321 cgggctttgt tccggtcagg gcattaaggg tacccgtcag atgaatgata ccgttttact
     1381 tcgggtttcc ggtcgcgaat ggggcggctg gacatccgtc cgtatcagtg cgggcattaa
     1441 ccgtattgcc cgggatttta atgttgccat taccacccgc tggcccggaa gccgggatta
     1501 tcaaccccgg ataaaaaatg gtgagctggt tgaagtgctt atcggggatg agcctgtgct
     1561 caccggatat gtggaggcac taccgcttcg ttatgacgcc agcagcgtca gcatgggaat
     1621 tgtcgggcga agcaaaacag ccgatctggt tgactgctct gctttgccac tccagcagag
     1681 cggaaaaaac ctgctcagaa tagtcagtga actggctgcg ccatttggca tcaccgttgt
     1741 tgatgctggc gtgccgcaga cagcggtgat tgatgcccag ccggaacacg gcgaaaccgt
     1801 tgccgattgt cttaaccggt tactggggca ggttcagacg ctggcttatg acgatgaatg
     1861 cgggcgactg gttctgggaa aacccggaac aggtaaagcg gcgacggcgc tggtgctggg
     1921 agagaatatt ctttcctgtg acacggaaag aagcatcaga gagcggtttt ctgaatatca
     1981 ggtcagtggg cagcgcccgg gcaacgacga tgattttggt gaggccacca ttgccgcaat
     2041 acgtcagacc attcaggaca gtggcgtgac ccgttatcgc cctttgttga ttcagcagtc
     2101 aggcacagca acgacagcaa cctgtaaggc ccgttgtgaa tttgaagcgc gccaacgggc
     2161 tgcgcttacc cgtgagacaa catatacggt tcagggctgg cggcagggca gtggcgcgtt
     2221 atggcgtccg gggttatctg tcatcgtttt cgacccgctg aataattttg ataatgatga
     2281 actggtgatc gcagaagtta cctataacca ggacgaccgg ggcacgacga ctgaattacg
     2341 ggttggcccg gcagatgctt atctccccga gcctgttacc gccaggaaga aaaaaatgtt
     2401 gaggaggatt tctgatgaac gggttttctc ttcgtaatct gatttcccgg gctgtcatca
     2461 cggcggtgga ttccgccaga aagtgtcagt ctgtagggtt gaaaatgata gccggagatc
     2521 agaaacaaca cgttgagcac cttgaacctt atggttttac atctgccgca cagaacggtg
     2581 ctgagggcgt tgctttattt ccggcgggcg atcgttctca tggtgtggtt gtggtcgtgg
     2641 ctgacagacg ttaccggctg aaaggactga aacgtgggga agtggcgctt tatgacgatc
     2701 tgggacagtc cgttgtcctg acccgttccg gtattgtggt ggacggggcc gggaaggcca
     2761 taatttttaa aaacgcgcct aaagcgcgct ttgaaatgcc agtcgaatcc accgccgata
     2821 ttactgacaa ttgcgacagt ggcggactca gcatgcagca aatgcggcag gcctacaatg
     2881 cccacaaaca taccgaaaat ggtgatggtg gcgggatcac tgacacgccg gatcaaccga
     2941 tgggctgaaa atcatgatga ttaatgttaa cgggcgaccc gtgtcgaccg gggcttcgat
     3001 cgaccttctg acgcgtgctg tgattatttc gctttttaca tggcggcgtg ccgggcgtga
     3061 tgatgcacca cggatatttg gatggtgggg ggatacctgg cctgcggttc agaatgatcg
     3121 cacggggtcg cgtttgtatc tgttgcgacg cagcaagctg acaaataaaa ccccgcagct
     3181 tgccagagat tatgtccgtg aggcgctggc gtggatggtg gaggatggtg ttgcttcccg
     3241 tcttgatatt aacgctgtcc ggaccgggac agactcgctg gcacttgcca ttaccattta
     3301 ccagcgtgac ggcaatattc acaacattat ttttgatgat atctggagtg aactgaatgg
     3361 ctgacagtca gttttatcgc cccggcctcc cgcaacttat ttctatgatc cggagcgatt
     3421 tattaacccg ctttgagcag gatacgctgc ttcgtcgtat ggatgcggaa gtgtatgccc
     3481 gtgtacaggc tgcagccgta cacacgttgt acgggtatat cgattatctt gccagaaatc
     3541 tgttaccgga cgtgtgtgat gaagactggc tgtaccggca cgccagaatc aaacgctgcc
     3601 cgcgaaaaga agcggtggca gcccggggat ttgtgcgctg ggatggcgta gaggggacgc
     3661 cggtattgcc agcgggaacg cagatccagc gtgataatca ggtgaccttt accacgacgg
     3721 cgacggtgac cgcagccgat ggtcttctcc gggtgcctgt tgtggcagac gaaccgggat
     3781 cggcggggaa tacggatgat ggtattgcca tgcaactggg aacacccgtc agtggtctgc
     3841 cgtccacagg gtacgctgac accattacag acggtgaaga tattgaaaat ctggaaatat
     3901 ggcgtgcccg cgttatggaa cgttattact acattccaca gggggg
//
LOCUS       DAZPJP010000286         3910 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPJP010000286 DAZPJP010000000
VERSION     DAZPJP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.289"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            26..592
                     /locus_tag="ACHU9J_004163"
     CDS             26..592
                     /locus_tag="ACHU9J_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1907559.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            596..1171
                     /locus_tag="ACHU9J_004164"
     CDS             596..1171
                     /locus_tag="ACHU9J_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1907560.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1213..2265)
                     /locus_tag="ACHU9J_004165"
     CDS             complement(1213..2265)
                     /locus_tag="ACHU9J_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1907561.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHU9J_004166"
     CDS             2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHU9J_004166"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1907562.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3582..3767
                     /locus_tag="ACHU9J_004167"
                     /pseudo
     CDS             3582..3767
                     /locus_tag="ACHU9J_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          988 c          952 g          999 t
ORIGIN      
        1 tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
       61 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
      121 tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca
      181 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat
      241 atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc
      301 tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc
      361 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc
      421 cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt
      481 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
      541 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
      601 aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc
      661 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc gcatccagca
      721 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
      781 cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
      841 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ccgcaaaata
      901 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
      961 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     1021 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     1081 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     1141 tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat ggcacgcatc
     1201 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     1261 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     1321 tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc
     1381 gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     1441 gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata tacgttcgcc
     1501 catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc cctgctcgcg
     1561 cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt tgaatccgtt
     1621 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acatgacgat
     1681 ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt
     1741 atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     1801 catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     1861 atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacaatgcg
     1921 atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     1981 caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac
     2041 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     2101 aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     2161 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa ccaacatttt
     2221 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     2281 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     2341 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     2401 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     2461 gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     2521 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     2581 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt
     2641 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     2701 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggactga
     2761 tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga
     2821 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     2881 tccattttct gacactggaa ctgggcgcgc ggcagtttgg tatgcaggaa ccggggattg
     2941 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     3001 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa
     3061 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg
     3121 tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     3181 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     3241 aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa actaccgccg
     3301 cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag tatatgtggt
     3361 tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa atctcccctg
     3421 ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat ttagcgataa
     3481 aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct
     3541 tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtggaaatg
     3601 acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg
     3661 gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag cttggcgtta
     3721 ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagtataata ccttcctaaa
     3781 tacactacct gagagaagag atgaaaagag ctatcagcta catgaggttt tcaactgaga
     3841 agcagataaa aggggactct atacgaagac agtctaagct ggtcactgac tggttatcaa
     3901 agaaccctga
//
LOCUS       DAZPJP010000287         3853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPJP010000287 DAZPJP010000000
VERSION     DAZPJP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.290"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHU9J_004168"
     CDS             complement(10..165)
                     /locus_tag="ACHU9J_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1907563.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHU9J_004169"
     CDS             complement(368..775)
                     /locus_tag="ACHU9J_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1907564.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHU9J_004170"
     CDS             852..1079
                     /locus_tag="ACHU9J_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1907565.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHU9J_004171"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHU9J_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2355
                     /locus_tag="ACHU9J_004172"
                     /pseudo
     CDS             1562..2355
                     /locus_tag="ACHU9J_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2362..3108
                     /locus_tag="ACHU9J_004173"
     CDS             2362..3108
                     /locus_tag="ACHU9J_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1907566.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3123..3545
                     /locus_tag="ACHU9J_004174"
     CDS             3123..3545
                     /locus_tag="ACHU9J_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1907567.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3603..>3853
                     /locus_tag="ACHU9J_004175"
     CDS             3603..>3853
                     /locus_tag="ACHU9J_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907568.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL"
BASE COUNT         1013 a          820 c         1075 g          945 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
     2041 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
     2101 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
     2161 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
     2221 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
     2281 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
     2341 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
     2401 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
     2461 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
     2521 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
     2581 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
     2641 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaact tggtaccggg
     2701 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     2761 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     2821 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     2881 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     2941 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     3001 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     3061 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     3121 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     3181 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     3241 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     3301 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     3361 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     3421 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     3481 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     3541 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     3601 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     3661 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     3721 acgcctctgt acgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcgg
     3781 ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga ctggcagatg
     3841 atgtaaggct att
//
LOCUS       DAZPJP010000288         3832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPJP010000288 DAZPJP010000000
VERSION     DAZPJP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.291"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHU9J_004176"
     CDS             complement(<1..265)
                     /locus_tag="ACHU9J_004176"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1907569.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="ACHU9J_004177"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="ACHU9J_004177"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1907570.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="ACHU9J_004178"
                     /pseudo
     CDS             complement(725..997)
                     /locus_tag="ACHU9J_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHU9J_004179"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHU9J_004179"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1907571.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3733)
                     /locus_tag="ACHU9J_004180"
     CDS             complement(2693..3733)
                     /locus_tag="ACHU9J_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1907572.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT          999 a          932 c          916 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcatta cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAZPJP010000289         3827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPJP010000289 DAZPJP010000000
VERSION     DAZPJP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.292"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHU9J_004181"
     CDS             <1..196
                     /locus_tag="ACHU9J_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907573.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHU9J_004182"
     CDS             207..374
                     /locus_tag="ACHU9J_004182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907574.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHU9J_004183"
     CDS             371..715
                     /locus_tag="ACHU9J_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907575.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHU9J_004184"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHU9J_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1907576.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHU9J_004185"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHU9J_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHU9J_004186"
     CDS             1710..1988
                     /locus_tag="ACHU9J_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907577.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHU9J_004187"
     CDS             1990..3048
                     /locus_tag="ACHU9J_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1907578.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHU9J_004188"
     CDS             3049..3429
                     /locus_tag="ACHU9J_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1907579.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>3827
                     /locus_tag="ACHU9J_004189"
     CDS             3426..>3827
                     /locus_tag="ACHU9J_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1907580.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          853 c         1046 g          894 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg cctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZPJP010000290         3736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPJP010000290 DAZPJP010000000
VERSION     DAZPJP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.293"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHU9J_004190"
     CDS             complement(<1..181)
                     /locus_tag="ACHU9J_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR1907581.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHU9J_004191"
     CDS             425..682
                     /locus_tag="ACHU9J_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR1907582.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHU9J_004192"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHU9J_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1907583.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHU9J_004193"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHU9J_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1907584.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHU9J_004194"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHU9J_004194"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1907585.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3736)
                     /locus_tag="ACHU9J_004195"
     CDS             complement(3307..>3736)
                     /locus_tag="ACHU9J_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1907586.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          887 c          893 g          954 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatttc
//
LOCUS       DAZPJP010000291         3709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPJP010000291 DAZPJP010000000
VERSION     DAZPJP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.294"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..363
                     /gene="trbI"
                     /locus_tag="ACHU9J_004196"
     CDS             <1..363
                     /gene="trbI"
                     /locus_tag="ACHU9J_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1907587.1"
                     /translation="DVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNAPMTAAFNM
                     KQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPAVVQGAP
                     DITREIQQDIARRMRAEP"
     gene            360..671
                     /locus_tag="ACHU9J_004197"
                     /pseudo
     CDS             360..671
                     /locus_tag="ACHU9J_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            666..1664
                     /locus_tag="ACHU9J_004198"
                     /pseudo
     CDS             666..1664
                     /locus_tag="ACHU9J_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            1684..2431
                     /gene="traX"
                     /locus_tag="ACHU9J_004199"
                     /pseudo
     CDS             1684..2431
                     /gene="traX"
                     /locus_tag="ACHU9J_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2486..3046
                     /locus_tag="ACHU9J_004200"
     CDS             2486..3046
                     /locus_tag="ACHU9J_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1907588.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            3176..3388
                     /locus_tag="ACHU9J_004201"
     CDS             3176..3388
                     /locus_tag="ACHU9J_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1907589.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          871 a          909 c         1199 g          730 t
ORIGIN      
        1 gatgttacag ccccccgacg cagccactgg tggtggacgg tgccgggatg cctggcaatg
       61 gtgttactga acgcagccgt cagttacggc attgtcagac tgaacgcacc gatgactgcc
      121 gccttcaaca tgaaacagac tgtggatgca ttttttgaca gcgccagtca gaaacaactg
      181 tcagaagcac agtcaaaagc cctttcagcg cgttttaaca cggcactgga agccagcctg
      241 caggcgtggc agcagaaaca ccacgcggtc attctggtgt cgcctgccgt ggtacagggg
      301 gctccggata tcacccggga aatccagcag gatattgccc ggcgaatgag agcggaacca
      361 tgagatgccg ggggctgatt gccctgctga tatgggggca gagtgtggcc gccgccgatc
      421 ttggcacctg gggtgatctg tggccggtaa aggagccgga catgctgacg gtgatcatgc
      481 agcgcctgac ggcactggag cagtccggtg agatgggccg gaaaatggat gcctttaagg
      541 agcgggtgat ccgcaacagt ctgcggcccc ctgccgtgcc cggtatcgga cgcacggaga
      601 aatacagcag ccggttgttt gacccgtccg tcagactggc tgcggatatc cgggataacg
      661 aagggcgggt ggccggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
      721 tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat gccgtacaga
      781 aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcagagc
      841 ggctgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc gttctccgtc
      901 aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga cgtaaatatc
      961 cgcaaccgta tgtggcactg ccggcgtttg accgtaacgg caagtccgcc ggtatctggc
     1021 tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggtcggg
     1081 taaaaggcag cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac ggagaaagcc
     1141 tgctggctga taatatgcag gatggcgtcc ggattgcccg tgataatcct gacagtggtg
     1201 tggtggtaag cattgccggt gaaggccgtc cgtggaatcc cggtgccatc accgatggcc
     1261 gcgtgtgggg ggatatcccg gacaacagcg tccagccggg agccggaaat ggcgaaccgg
     1321 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     1381 gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     1441 aaacagagct ggctgtcagg gaaattgccg ggcaggagcg tgaccgggct gccataactg
     1501 aacgggaagc cgcgctgccg gagagtgtgc tgcgtgaatc acaacgggag cgggaagcgg
     1561 tccgggaggt tgcccgggaa aatctgctgc aggagcgtct gcagcagatg gagcgggata
     1621 tggttcgtga cctgcagaaa gagaaaaccc cgggcggaga ctgatacagg aagacaaaca
     1681 ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat
     1741 acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg
     1801 gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg
     1861 atgtttctgg ccgggcgagg agcctttccg ctttttgccc tggtgtgggg gctgaatctg
     1921 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg gggaattatt
     1981 gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat cctgtttgcc
     2041 tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca
     2101 gcggccattc ttctgatggc cctgttgggg gcctttgtcc ggcaccagtt acggcattgc
     2161 cgggctgctg atgctggcag tcagccaccg actgtaccgg gcggaagaca gagcggaacg
     2221 tctggcgctg gtcgcctgcc tgctggctgt tattccggcg cttaaccttg ccaccagtga
     2281 tgcagcggcg gtggccggtc ttgtgatgac ggtgctgacc gttggtctgg tgtcgtgtgc
     2341 aggaaaatca ttaccccgtt tctggcccgg ggattttttc ccggtattct acgcctgtca
     2401 tctggctgtg ctgggcgttc tggcgctgtg acgggtgtgg tatctttgac cgtaacagga
     2461 tgattcgtca gaggcagaac acagcatgac agagcagaag cgaccggtac tgacactgaa
     2521 gcggaaaacg gaaggggaga cgcctgtccg cagccggaaa accatcatca atgtcaccac
     2581 gccaccaaag tggaaggtga aaaagcagaa gctggccgag aaagcagccc gggaagcaga
     2641 gctggcggca aaaaaagcgc aggccagaca ggcactgtcc atttatctga acctgcccac
     2701 gctggatgag gccgtgaata ccctgaaacc ctggtggccg ggattatttg acggtgacac
     2761 cccccggctt ctggcctgcg gtatccggga cgtgttactg gaagacgtgg cgcagcggaa
     2821 tatcccgctc tcgcataaaa aactgcgcag ggcgctgaag gccatcaccc gttcagaaag
     2881 ctatctgtgt gccatgaaag ccggtgcctg ccggtatgac acggaagggt atgtgacgga
     2941 gcatatttct caggaggagg aagcgtatgc ggcagcgcgt ctggataaaa tccgccgcca
     3001 gaaccggata aaggcagaac ttcaggccgt gcttgatgag aaataaaaaa aagcctccat
     3061 cagagaggag gcagggaaat aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc
     3121 cagtccactg aattatttat cacaataata ctgacaacta aagggggaca agaaaatgaa
     3181 tggattcaga aacagttcac ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg
     3241 ggccgttatt ctggaagtca gtggacgctg gatggaagcg gcagaagcat ggcgacgggc
     3301 tgcctgtgtt gcgcccagga cagactggca gcagtttgcc cgaaaaaggg ctgaacactg
     3361 ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag ccttcattac tccaggagtc
     3421 agaaggctga aaggagttta tgagatttgc tttttttaat atccggtcgg gcagtatgac
     3481 cggacagttc tgagtagcac agagtttttt ccgtattaag tcgtgatttt atattttgtg
     3541 attaatttca caaaataagg tgttattcag tgtgtgctgc aatattcagg atgcctgaaa
     3601 tacagatgct atttatctga tatggagaat aacatgagga aatagtaagg cagtatgact
     3661 ttccgtgaga cagatgctgg ccaacgtggg atatttaacg cgggttaac
//
LOCUS       DAZPJP010000292         3683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPJP010000292 DAZPJP010000000
VERSION     DAZPJP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.295"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            69..1010
                     /locus_tag="ACHU9J_004202"
                     /pseudo
     CDS             69..1010
                     /locus_tag="ACHU9J_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1053..1118
                     /locus_tag="ACHU9J_004203"
                     /pseudo
     CDS             1053..1118
                     /locus_tag="ACHU9J_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310208.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1118..2439
                     /gene="hipA"
                     /locus_tag="ACHU9J_004204"
                     /pseudo
     CDS             1118..2439
                     /gene="hipA"
                     /locus_tag="ACHU9J_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2623..2724
                     /locus_tag="ACHU9J_004205"
                     /pseudo
     CDS             2623..2724
                     /locus_tag="ACHU9J_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            2922..3459
                     /locus_tag="ACHU9J_004206"
                     /pseudo
     CDS             2922..3459
                     /locus_tag="ACHU9J_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3501..>3683
                     /locus_tag="ACHU9J_004207"
     CDS             3501..>3683
                     /locus_tag="ACHU9J_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907590.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
BASE COUNT         1019 a          764 c          904 g          996 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaact tttttaagat
     1021 tttacagtcg cttgaactct caatgacgct atatgacgct atgcgacgcg aaaaatgcct
     1081 cgccagaagc aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat
     1141 gaacaaccag cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta
     1201 tgcaccggag tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca
     1261 gagggggaat atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag
     1321 cccgattgta cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga
     1381 tttgttgtca gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga
     1441 aaccgtaacg cgtccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt
     1501 attaacggct tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg
     1561 catctcggtt gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg
     1621 cattccgaaa ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag
     1681 gcagcccaat gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct
     1741 gctggcgaaa gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt
     1801 gcgcgcgtta gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact
     1861 tcgcttgcca caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga
     1921 atcagatgga ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc
     1981 gctgaaagat cgctatgatt ttatgaagtt tcaagtgttc cagtggttga ttggcgcaac
     2041 ggatggccat gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac
     2101 gccattttac gacatcattt cagcatttcc ggtccttggc ggtacgggaa taacatcagc
     2161 gatctcaaac tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa
     2221 atttatccgc gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg
     2281 catgaaatcc taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact
     2341 tcattaccga cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg
     2401 ttgcatggac ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt
     2461 taatgagcaa tcatgaccgg ccaacgactg gattctccct tggtttatgt ttgtgcgcat
     2521 aatgtatatt atgttaaatc atgtctgagt gatgcgaagt gcttttctgg tagtcgttat
     2581 tcgttcagat taggtgtcct tgtgatttaa agatgttatc gtggcacgaa tatactgcca
     2641 ttccaggctc gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg
     2701 accttcaaag ttcaatatct ataaacaaca gtagttaaac ttactgttat ttaaaatatt
     2761 tactttgtag caaggttttc ctattaaaag agaaccaaca tttattacaa acgcattaag
     2821 tctggaaacc cggcgactcg ccaatgtatt agcaacatta atcgattgtc tgatgtaacg
     2881 gttttcccgc ttatcctctt aacacatgga gaatgcatca gatgcaaact acgcgtaccc
     2941 cctactctat ttcctttatg gcgacagtgt tacttttatt gctctttgct tgccatagca
     3001 ccgttgccaa cgcagctgtc gcactggaat acgctgcgga taatcaacgc tactaatcat
     3061 cagttaccag gcgaccgtga aagcctgttc tgggtgaatg ttaaagctat tcctgctatg
     3121 gaaaaagacc agaagaatga gaatacatta cagctggcaa ttattagccg cataaaaatg
     3181 ttttatcgcc ccacgaacct ggcaatggca cctgaagaag cgcctgcaat gcttcggttt
     3241 cgtcgtagcg gtagcaaact cactcttatt aatccaacgc cctatttcat taccgtaaca
     3301 aatatgaaag caggaaatag caatctccca aacaccatgg tgccgccaaa aggtgaagta
     3361 tcggtggata tcccgcatgc tgcaaccggg gatatcagtt ttcagacgat caatgattac
     3421 ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag ccagtgacct aattaataat
     3481 aaactcaatc ggaattaata atgacagcct ttcgtgccgc atttaaggca tatcgtatgc
     3541 atcaggtgtt aatactgcct cgctttgcac ggcttaccat tgcccttggc ttagcaactg
     3601 cggtatttcc cgttgacgca gagtattatt ttaatccacg gtttttaagc aatgatcttg
     3661 ctgaatctgt cgatttatcc gcc
//
LOCUS       DAZPJP010000293         3674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPJP010000293 DAZPJP010000000
VERSION     DAZPJP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.296"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHU9J_004208"
     CDS             <1..95
                     /locus_tag="ACHU9J_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1907591.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHU9J_004209"
     CDS             244..1491
                     /locus_tag="ACHU9J_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR1907592.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHU9J_004210"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHU9J_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR1907593.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHU9J_004211"
     CDS             complement(2343..2687)
                     /locus_tag="ACHU9J_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907594.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="ACHU9J_004212"
     CDS             2929..3531
                     /locus_tag="ACHU9J_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907595.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAZPJP010000294         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPJP010000294 DAZPJP010000000
VERSION     DAZPJP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.297"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHU9J_004213"
     CDS             complement(89..652)
                     /locus_tag="ACHU9J_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1907596.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHU9J_004214"
     CDS             complement(756..974)
                     /locus_tag="ACHU9J_004214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907597.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHU9J_004215"
     CDS             complement(1133..1519)
                     /locus_tag="ACHU9J_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1907598.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHU9J_004216"
     CDS             complement(1509..1880)
                     /locus_tag="ACHU9J_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1907599.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHU9J_004217"
     CDS             complement(1880..2137)
                     /locus_tag="ACHU9J_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907600.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHU9J_004218"
     CDS             complement(2134..3525)
                     /locus_tag="ACHU9J_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1907601.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHU9J_004219"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHU9J_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1907602.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZPJP010000295         3605 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPJP010000295 DAZPJP010000000
VERSION     DAZPJP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.298"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            846..1532
                     /locus_tag="ACHU9J_004220"
                     /pseudo
     CDS             846..1532
                     /locus_tag="ACHU9J_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1865..2740
                     /locus_tag="ACHU9J_004221"
     CDS             1865..2740
                     /locus_tag="ACHU9J_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1907603.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2748..3524
                     /locus_tag="ACHU9J_004222"
     CDS             2748..3524
                     /locus_tag="ACHU9J_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1907604.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          997 a          826 c          838 g          944 t
ORIGIN      
        1 ttgatatatc aaaattgttt tcctctattt ttgtgtagat agatgttaat ggcactgggt
       61 gcaatcaatt aagaaaattg acgccagact aattattaat aatccagtta tctttttatt
      121 catatcatct cctttccatt ggttaattta tcggaaattc atccccacta actaaaacga
      181 tccacacaat aaaactaaat ctaattgcag gcaatcaatt ccagtcgata tatcatctgt
      241 atggaaatca tcgctcatca ctcatcgcag cctgaaagcc cagagcaacg gggcaggtct
      301 gcatataagt ggttacacgg cagaattcca gagggttctg tcactaaatt cagtcactga
      361 caaatttgca tgatgttgaa tagacgagat tttgtgtcga aatagttgtt tcagtaactg
      421 tcactcaatc ggtggtttat gtgactatat tcgcaaaaac tggtcgcaga aaagtcctgt
      481 tttcagcttg gaaacccggt aatcagaata atggataggg gaacaaaaat ctctgtatag
      541 aacgctccat tgagggagag aaaacgtgtc attttgaccc gttcggtatt cagaacacac
      601 aaaaccgatc gcgggtcgct ttcgtgcata gttatgcaac gcgctaaaaa cgattctgac
      661 gcatttttta tgtttaccct gttttttctt gtctttttga gttttttgcg tcagagagcg
      721 tctgagaggg cacgtacaac gggagttatg gtaaatggat cgggttttcg ggaaggttcg
      781 acaggatttg ccgttgggtg tagtgtaagc gactgaaaaa caaacgcccc tgaaatcatg
      841 tccagatccg gcagaaccag cctcatgcgc agttctggct ggtttttgac gttgatcgcg
      901 agggggcagc gattgactgg agcgaccgga acgctcccgc gccgaacatc accgtaaaaa
      961 atcctgtgaa cggtcatgct cacctgctct acgcgctcaa tatcgccgtg agaaccgcgc
     1021 cggacagctc ggttaaggcg ctgaaatacg ccgctgcgat tgaacgttcg ctgtgtgaga
     1081 aactttgtgc tgatgtgaac tacagcggac tgatttgtaa aaatccgttc cacctggaat
     1141 ggcaggtgat ggagtggcga gaggaagcct ataccctcga tgaactggct gattatctcg
     1201 atttgagcgc ctcagcgcgc cgtagcatcg ataaacatta cgggatgggg cgaaactgcc
     1261 acctgttcga aatgacgcgc aaatgggctt acagggcgat tcgtcagggc tggcccgaat
     1321 tctcacagtg gcttgatgcc gtgattcagc gtgtcgaaat gtacaacgca tcgcttcccg
     1381 ttccgctttc accgcctgaa tgtcgggcta ttggcaagag cattgcgaaa tacacgcaca
     1441 ggaacttcac gccggaaact ttcgcacagt atgtggctga tacgcacaca ttaacgtacg
     1501 ttttcgttcc attggccctc accccccgat aacgacccag gtagcgcgcc gtattccccc
     1561 cgccggccgt tttcttcgac atgtacatca ggcgcggaac tcatcggggc gcgggaatga
     1621 aatcccattc agactccgga tagattcaag caagcccact tatcgtcaaa atcggtgttg
     1681 caaaaacggg agtgaccata gatccgtttt ccaagcggac cccataatat cggctggtaa
     1741 acatcactcg ctagtgcatt ctttttgttg gtaatgataa tcatttacat tcattctttg
     1801 atgttttatc ataattaatg ttttgtcaat aaatatatgg aaacatattg tttcagatgg
     1861 gaatatgaga aagttcatcc ttatttctat gataacgtta gctagtgtta gtctgggagc
     1921 ctgcagacag aatgtcacca taaagcagga tgcgccaggg cagaaagctt tcctgactga
     1981 cgggcagatt tacgatttgc acagtggcaa aattatttct agcagcgagc ttttggcgga
     2041 tcttgctacc gctcgacatc ttattattgg tgaaaaacac gataatgccg agcatcatca
     2101 gattgagctt tggcttattc aaaatttgct catccagcga ccacagggga gtgtgttgct
     2161 tgagatgttg acgtcagagc aacagccccg agttaaccaa gttaaatgtt ggctgaaaga
     2221 taatcctgtg gttcgagata gccgtgttca ggaactactt aactggcaaa aagggtggtc
     2281 ctgggagatg tatggcgaca tcgtcatgca gctattacgt gggccttacc cgctgctcaa
     2341 tgccaacatt ggccgggagc agatgttggc actgtacaaa aaaaacgaat ttcctaaagg
     2401 aaaaaaatcc actgcacctg tggtacaaga agctctgcgt gaaaccatca tatctatgca
     2461 tgaaggcaac cttgaaagcc agcaactgac gtcaatgctc tctattcagc agcagcggga
     2521 tcgctacatg gcccgccagc ttctgagtgc cccggtaccg tcgttgttga ttgcaggtgg
     2581 ttatcacgcg tcaaaaagca tgggcgtacc gttacacatg gaagatcttg ccaccggcac
     2641 ccatccagtg gtgttgatgc ttgcagaaaa aggaatgaac atcacagtag atcatgctga
     2701 ctacgtgtgg tttgtagcgc cagatacaac aaaaaggtaa accatggatg atgaatattc
     2761 tccacttctc acagtcggta aaatggagca gctggtttat ctgttctcta ctgcttcatg
     2821 gactgatttt ccttgcgttt atctggcgtt tttccgaagt tcagcccgct atgtctcctg
     2881 caccggcgat catgctgcaa tgggcagaaa ttatagaggc tccttcttct ccactttcgc
     2941 ttccggtggg aatcgctcaa caggagtccg cagtgacaga agaaaagcaa caaactgagg
     3001 acagacaaca acgtcctgtc actgaagaca gtgatgcaac cattgaaata actaggaaga
     3061 aaaagtcatc cgatggagaa aagaaaaaga cacgtccacc tcgtaagata aaggctcaaa
     3121 ccagcgacag caaccccaca gccgtttcca gtaatgctgc acctcaggct ctggtcgaat
     3181 cgtcccgtat tgctgcgcct tttaacagtg actcaacaaa gcgagataat tctgaggcat
     3241 cttgggaaag cagagtaaaa ggccacctaa accgttataa acgttaccct ggcgatgcca
     3301 gaaaacgtgc acgaaccggc acagccgtcg tgacttttac cgtaaacacg gaaggcacta
     3361 ttgtcagtag tttcttggaa atctcttcag gaaccctttc tcttgaccgc gaagctattg
     3421 ctgttttaga acgcgctcaa ccacttccca aaccaccgcc tgaaatattg gagggaggat
     3481 tatttaaagt aaaaatgccg attaccttta agttgaagga gtaaagggat aattggccgt
     3541 tcaagttcat ttaactgaaa tttaattttc atttttatgc tgaacatttt atcaaacttt
     3601 tccac
//
LOCUS       DAZPJP010000296         3583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPJP010000296 DAZPJP010000000
VERSION     DAZPJP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.299"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(75..1472)
                     /gene="ccp"
                     /locus_tag="ACHU9J_004223"
     CDS             complement(75..1472)
                     /gene="ccp"
                     /locus_tag="ACHU9J_004223"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1907605.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1877..3526
                     /locus_tag="ACHU9J_004224"
     CDS             1877..3526
                     /locus_tag="ACHU9J_004224"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1907606.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          910 a          927 c          852 g          894 t
ORIGIN      
        1 tgataatatt aaaaacagac tttattatcg taataaaaat cgcccaaaca ttcctgttcg
       61 ggcgatcaaa ttaattattg tttatcctgc atatacggcg tgtacacccc gttcagactg
      121 tgcaggaaag ctacgatatc atccacatcc tcctgcggca gctctttgcc tacctgatag
      181 cgcagcatca gtttcaccgc cccgtccagc gtcggcacgt caccgcgatg gaagtacggt
      241 gccgttaaag caacgttacg taaaccgggt actttctgac gcaatttatc acgctcttct
      301 ttagtaacat tcatacgacc aatatccgcc gccgtaattt ccccaaagtt aaagtctttt
      361 ttcagcccca acggttcaaa ggaacgtcca ccgagaataa taccaccatg acaagttgca
      421 catttattat ctttaaataa ttgatagcct tttttctgtt gcgccgtcag cgcattttca
      481 tctccacgca accatttatc aaatggggaa tccggcgtaa ttaatgtttt ctcaaattca
      541 gcaatggcat cagtaatatt ttcgccactg aaaccttgcg gatagacgcc gaggaactgc
      601 gctttaagct gcggatcttt ttccagctta gcaataattt cgtcccaaga tttcgacgcc
      661 atttcaatcg ggttcaacgg cggtccacca gcctgatcct gcaatgttgc cgcacgacca
      721 tcccagaact gctcaacgtt aaatactgag ttaaataccg tcggcgcgtt aatcggccca
      781 actgcgccac caacaccaat cgatgttttt ctgccatcga cgccccccgc attcaacgca
      841 tggcaatgag cgcatgaaat ggtgctatca gccgataaac ggggatcgtg atacagcgca
      901 aagcccaacg ccactttttg cgcatcggta ggcagttttt gcgggatggg ctgcaccggt
      961 tcattgcgat gctccggagc agtatcattg ctggcgtaat attccgcgcg ctgttttgca
     1021 atccaggcca gtatttccgc ccgctcttca tcactcacct tacccgccca gtgaagcgcg
     1081 gtataacgcg ttggcggcat agtttcatac tgcatcaccc attcaatctt attcagatcg
     1141 ctttgcgaaa cgggtttatc agccagcagt gccgcacgca cggcctcgag gttaaaagat
     1201 ttatatccaa gcttaatgtc gtaatccatc aactgtttcg cgccaggaat ataataatag
     1261 gcgggtaatt ctgccgaagg cgtgtggcaa tagtcgcatc ctttttcgcg gagaaaacct
     1321 aaaaccttat tattttcact gacagcggat gcctgaacat cggcctgttt actgcgttta
     1381 ttatcgtggt accacacata accagataac cctaaatagc aaatcgcgac gccagccagg
     1441 ccgatcgcgg taatacgtga gaccattttc attattcttc cctcacggtg gtgagttatt
     1501 gtgacccaga aaattttcca gaatgctgca aacgtctctg cgagacaaag ccatcataaa
     1561 aaacagtgag atacgctttt tgataggaat caatcaatta catgaatata aactatcaat
     1621 aagatagcct gtgcctatta cccagatcag gaatggcata tcatgattgc ggcgagagca
     1681 ggattgaatg ttctgaaaat gaaaaacaac acgaaatccg tatttttata aattccatgg
     1741 atattagtgc gttatatttt ttgacaggtt aataaacgtg atccaccgca cgctttgtcg
     1801 cccaccaggc ggagcgaatg actaccctta aagaaaagcc cgataattag cgacgaattt
     1861 cggaggttgg atccttatgc tcaatcagaa aattcaaaac cctaatccag acgaactgat
     1921 gatcgaagtc gatctctgct atgagctgga cccgtatgaa ttaaaactgg atgagatgat
     1981 cgaggcagaa ccggaacccg agatgattga agggctgccc gcctctgatg cgctgacgcc
     2041 tgccgatcgc tatctcgaac tgttcgagca tgttcagtcg gcgaaaattt tcccggacag
     2101 taaaaccttt cccgactgcg cacctaaaat ggacccgctg gatattttaa tccgctaccg
     2161 taaagtgcgc cgtcatcgtg attttgactt gcgcaagttt gttgaaaatc acttctggct
     2221 gccggaggtc tactccagcg agtatgtatc ggacccgcaa aattccctga aagagcatat
     2281 cgaccagctg tggccggtgc taacccgcga accacaggat cacattccgt ggtcttctct
     2341 actggcgctg ccgcagtcat atattgtccc aggcggccgt tttagcgaaa cctactattg
     2401 ggactcctat ttcaccatgc tggggctggc ggaaagtggt cgggaagatt tgctgaaatg
     2461 catggccgat aacttcgcct ggatgatcga aaactatggt cacatcccca acggcaaccg
     2521 cacctattat ttgagccgat cgcaaccacc ggtttttgcg ctgatggtgg agttgtttga
     2581 agaagatggt gtacgcggtg cgcgccgcta tctcgaccac cttaaaatgg aatatgcctt
     2641 ctggatggac ggtgcagaat cgttgatccc taatcaggcc tatcgccatg ttgtgcggat
     2701 gccggacgga tcgctgctca accgttactg ggacgatcgc gacacgccgc gtgacgaatc
     2761 ctggcttgag gacgttgaaa ccgcgaaaca ttctggtcgc ccgcccaacg aggtgtaccg
     2821 cgatttacgc gcgggggcgg cctccggttg ggattactct tcccgttggc tgcgtgatac
     2881 tggtcgtctg gcgagcattc gtaccaccca gttcatcccc atcgatctga atgccttcct
     2941 gtttaaactg gagagcgcca tcgccaacat ctcggcgctg aaaggcgaga aagagacaga
     3001 agcgctgttc cgccagaagg ccagtgcccg tcgcgatgcg gtaaaccgtt acctctggga
     3061 tgatgaaaac ggcatctacc gcgattacga ctggcgacgc gaacaactgg cgctgttttc
     3121 cgctgccgcc attgtgccgc tctatgtcgg catggcgaac catgaacagg ccgatcgtct
     3181 ggcaaacgcc gtacgcagcc ggttactgac acctggcggg attctggcaa gcgagtacga
     3241 aaccggtgaa cagtgggata aacccaacgg ctgggcaccg ttacaatgga tggcaattca
     3301 gggatttaaa atgtatggcg atgaccttct gggtgatgaa atcgcgcgca gctggctgaa
     3361 aacggtgaat cagttctatc tggaacagca caaaatgatc gagaaatacc atattgccga
     3421 tggtgttccc cgcgaaggcg gcggtggcga gtatccgttg caggatgggt ttggctggac
     3481 taacggtgtg gtacgccgtt taattggttt gtacggcgaa ccataatatt tttacagcca
     3541 gccgctaact tcctgctggc tgtaaaatta tcctcttcag gag
//
LOCUS       DAZPJP010000297         3546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPJP010000297 DAZPJP010000000
VERSION     DAZPJP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.300"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHU9J_004225"
     CDS             complement(<1..143)
                     /locus_tag="ACHU9J_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1907607.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHU9J_004226"
     CDS             complement(189..530)
                     /gene="yiaB"
                     /locus_tag="ACHU9J_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1907608.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHU9J_004227"
     CDS             complement(699..2153)
                     /gene="xylB"
                     /locus_tag="ACHU9J_004227"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1907609.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHU9J_004228"
     CDS             complement(2225..>3546)
                     /gene="xylA"
                     /locus_tag="ACHU9J_004228"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1907610.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          926 a          982 c          784 g          854 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaaa aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 gtacggtaat cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta
      961 cgcgccccgc caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca
     1021 tccatgccat ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg
     1081 gggccatgtt gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt
     1141 gtacgctcgc cggaaagata aggcagaaac caaactggct cgacactttc atcagcctgt
     1201 tgagctgcag cgattaaagc tgggacattg cacaggccgg ttaatttcgc ggcccaatcc
     1261 agacacgatg ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg
     1321 caaaagctgt gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag
     1381 acccccgacg tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact
     1441 gcaccagctg cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct
     1501 ttcgcaactt caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc
     1561 tggtcacgag ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca
     1621 tccagccaca tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc
     1681 agacgcaagt aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca
     1741 tgccgctgaa cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta
     1801 atcactcgtg attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg
     1861 tcgttccaca aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca
     1921 tgcatctggc cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga
     1981 gctttcattg cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt
     2041 ggatgcgggc gcgaaacggt cagcttttcc gtttgcgaat caaccacctc accctgctcg
     2101 ttgagcaaaa taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc
     2161 gttccttaaa aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta
     2221 gccgttattt gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac
     2281 tctgatgcac cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca
     2341 tttggccttt caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga
     2401 tgcgtttatc cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca
     2461 tcgtatccat cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac
     2521 gtactttggc atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat
     2581 acatcaccag cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt
     2641 gcgcatcgcc acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta
     2701 tttcatgatg gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa
     2761 tctctttttc cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat
     2821 attgatgttt agtcggttct tgcggtttcg gttcgataag caacgtgccc tggaagccga
     2881 ttttatgttt atgctcaacc accatctgca taaagcggcc cagttgttca cgctcctgac
     2941 gcaagtcggt atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt
     3001 caccgcccaa tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccagctga
     3061 agacttcagg atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt
     3121 tagcggttcc ccacagcagc ttcacgccgc tctcttcttg cttggctgcc aggacatcaa
     3181 ccatttgcgc aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca
     3241 catcgtggaa gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg
     3301 ctttacgctt cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc
     3361 ccacaccaaa catgtccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac
     3421 gcaagtgctc ttccatacgc ttacccaaca ccagttcgtc gggattgtag tgacggaatg
     3481 ctaacgggtt tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg
     3541 cttgca
//
LOCUS       DAZPJP010000298         3521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPJP010000298 DAZPJP010000000
VERSION     DAZPJP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.301"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHU9J_004229"
     CDS             complement(<1..1255)
                     /locus_tag="ACHU9J_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1907611.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHU9J_004230"
     CDS             complement(1412..1621)
                     /locus_tag="ACHU9J_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907612.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHU9J_004231"
     CDS             complement(1644..2354)
                     /locus_tag="ACHU9J_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1907613.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHU9J_004232"
     CDS             complement(2870..3193)
                     /locus_tag="ACHU9J_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907614.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
BASE COUNT         1019 a          743 c          585 g         1174 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg a
//
LOCUS       DAZPJP010000299         3468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPJP010000299 DAZPJP010000000
VERSION     DAZPJP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.302"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1347
                     /locus_tag="ACHU9J_004233"
     CDS             <1..1347
                     /locus_tag="ACHU9J_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907615.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKRPATMAQHNLA
                     NDLLRKMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQHEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYGVFMHLRY"
     gene            2232..>3468
                     /locus_tag="ACHU9J_004234"
     CDS             2232..>3468
                     /locus_tag="ACHU9J_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFR1907616.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLNTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNLSARTNEQKHANDALNALLK
                     EKENIRNQLAGINQKIAEEKRKQDELKATKDAINFTTEFLKSVSEKYGAKAEQLAREM
                     AGQAKGKKIRNVEEALKTYEKYRADINKKINAKDRAAIAAALESVKLSDISSNLNRFS
                     RGLGYAGKFTSLADWITEFGKAVRTENWRPLFVKTEAIIAGNAATALVALVFSILTGS
                     ALGIIGYGLLMAVTGALIDE"
BASE COUNT         1011 a          754 c          871 g          832 t
ORIGIN      
        1 atgccaatga tacttgtaaa gaagaatccc cggggcaaag tgattcggga gcttagcagt
       61 gaggaagaga ctgcagtaaa gacggtctgt ggactgaaaa ggccagcaac catggcacaa
      121 cataacctgg ccaatgatct tctccggaaa atgcgtgagt atgatgcatg gttacagtgt
      181 gactgtattc cgggagattc gccggccatg aattttgctg ctcttaaaaa caatacgggt
      241 actctttatc tgtccagttt taatcatgag catgcacctg aatgccccat gtatcgtcag
      301 ttatccggaa atgaagagga aacatccttc ggagcaagcc ggcatcccgt tagcacccgt
      361 ataaattaca gaaacttttt accacctgat gacagtaatt ctgttatcag actgcaggcc
      421 cgctctgctt accataatgg tgagcgctcc tccgttcgca agaaacgtcc gcgccttggc
      481 cgtttactcc tgtcgcttat tgaagatgca gggctgaata aactggattc cctggctaat
      541 ccccgtatcc ggactaatta tcgtgagtgt cttgatgcca tacggcaggt tacactacag
      601 catgagtata ttcggggaag agccttatct gaaattatcc atttccggcc aggaatgtct
      661 gaaaggtctc aggagcggtt aatggaaacg ctggagaatt cagaacgaca ctggcctgcc
      721 agaagaaagc atatgttctt tcagattttt atggcgcaac acatctgtcg tgatgctgtg
      781 gaaattcact gggcgaacgg taatattcag gtcatcagac ctgtgcgggg gatcagtatt
      841 aatggtgaag cgcagggcgg aatacgtccc ccttactggg ttattcttgc tttttgccgg
      901 agtgccgatg gcagaatcat ctgcagtgaa gggtatgctc atgctcttta tcaactgaca
      961 tgtccggtgc ctgtggacag caaactggaa cgaaacacgc tcactgctct tctgaatgtg
     1021 gccagctggc ttaaaagaaa gccaggtacg ccggaattaa gtctggaaag gcccctgttt
     1081 gatacagaag tttatgttaa tggtgaaaag aaatatgtac tgccggattt cattgtcaca
     1141 gcaagggctc ctgacggaaa gacggccaga gtggtcatcg aaacgatggg atatgaagac
     1201 agtgattact gcgcgagaaa atccaggcag cataccggca tgaagcagat tggtgttctg
     1261 cataccgatc caccgaaatg gctggataac gatcatcccc cttttaagaa acatatgtac
     1321 ggtgttttta tgcatctcag gtactgagat attttgtggc tcagttctgt aacttttccc
     1381 gtaacattgt ctgttgttac gggaaagtcc ggtttttgta ttgcaccaga gaatacccag
     1441 actgtgatgc tgccacagcg tcagctggct ttctgaacgg tgtaacatca cttcatgtta
     1501 atgataatca ctatcatcaa atcttgacat gccattttct ccttaataaa ttagtactgt
     1561 atatgtatcc atatgcgtaa gcagttaatt catttgtttt cctcagagga tgaaggagat
     1621 accgaatgtc tgaccctgta cgtattacaa atcccggtgc agaatcgctg gggtatgatt
     1681 cagatggcca tgaaattatg gccgttgata tttatgtaaa ccctccacgt gtcgatgtct
     1741 ttcatggtac cccgcctgca tggagttcct tcgggaacaa aaccatctgg ggcggaaacg
     1801 agtgggttga tgattcccca acccgaagtg atatcgaaaa aagggacaag gaaatcacag
     1861 cgtacaaaaa cacgctcagc gcgcagcaga aagagaatga gaataagcgt actgaagccg
     1921 gaaaacgcct ctctgcggcg attgctgcaa gggaaaaaga tgaaaacaca ctgaaaacac
     1981 tccgtgccgg aaacgcagat gccgctgata ttacacgaca ggagttcaga ctcctgcagg
     2041 cagagctgag agaatacgga ttccgtactg aaatcgccgg atatgatgcc ctccggctgc
     2101 atacagagag ccggatgctc tttgctgatg ctgattctct tcgtatatct ccccgggagg
     2161 ccaggtcgtt aatcgaacag gctgaaaaac ggcagaagga tgcgcagaac gcagacaaga
     2221 aggccgctga tatgcttgct gaatacgagc gcagaaaagg tattctggac acgcggttgt
     2281 cagagctgga aaaaaatggc ggggcagccc ttgccgttct tgatgcacaa caggcccgtc
     2341 tgctcgggca gcagacacgg aatgacaggg ccatttcaga ggcccggaat aaactcagtt
     2401 cagtgacgga atcgcttaac acggcccgta atgcattaac cagagctgaa caacagctga
     2461 cgcaacagaa aaacacgcct gacggcaaaa cgatagtttc ccctgaaaaa ttcccggggc
     2521 gttcatcaac aaatcattct attgttgtga gcggtgatcc gagatttgcc ggtacgataa
     2581 aaatcacaac cagcgcagtc atcgataacc gtgcaaacct gaattatctt ctgacccatt
     2641 ccggtctgga ctataaacgc aatattctga atgaccggaa tccggtggtg acagaggatg
     2701 tggaaggtga caagaaaatt tataatgctg aagttgctga atgggataag ttacggcaac
     2761 gattgcttga tgccagaaat aaaatcacct ctgctgaatc tgcggtaaat tcggcgagaa
     2821 ataacctcag tgccagaaca aatgagcaaa agcatgcaaa tgacgctctt aatgccctgt
     2881 tgaaggaaaa agagaatatc cgtaaccagc ttgccggcat caatcagaag atagcggaag
     2941 agaaaagaaa acaggatgaa ctgaaggcaa cgaaagacgc aattaatttc acaacagagt
     3001 tcctgaaatc agtttcagaa aaatatggtg caaaagctga gcagttagcc agagagatgg
     3061 ccgggcaggc taaagggaag aaaatacgta atgttgaaga ggcattaaaa acgtatgaaa
     3121 agtaccgggc tgacattaac aaaaaaatta atgcaaaaga tcgtgcagcg attgccgcag
     3181 cccttgagtc tgtgaagctg tctgatatat cgtctaatct gaacagattc agtcggggac
     3241 tgggttatgc aggaaaattt acaagtcttg ctgactggat cactgagttt ggtaaggctg
     3301 tccggacaga gaactggcgt cctctttttg ttaaaacaga agccattata gcaggcaatg
     3361 ccgcaacggc tcttgttgca ctggtcttca gtattcttac cggaagcgct ttaggcatta
     3421 tcgggtatgg tttactgatg gctgtcaccg gtgcgctgat tgatgaat
//
LOCUS       DAZPJP010000300         3443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPJP010000300 DAZPJP010000000
VERSION     DAZPJP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.303"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..305
                     /locus_tag="ACHU9J_004235"
     CDS             <1..305
                     /locus_tag="ACHU9J_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907617.1"
                     /translation="PRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYS
                     NNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            394..2262
                     /locus_tag="ACHU9J_004236"
     CDS             394..2262
                     /locus_tag="ACHU9J_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR1907618.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2299..>3443
                     /locus_tag="ACHU9J_004237"
     CDS             2299..>3443
                     /locus_tag="ACHU9J_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR1907619.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQIL"
BASE COUNT         1086 a          627 c          668 g         1062 t
ORIGIN      
        1 acccacgtgt agaactgtat taccgcttta accatgaaga agaatattac ccttctcacc
       61 gttttatgct tgttgttgat atgaagacag taatggtttt ttcacaacga gctgaatcac
      121 tggggttata ctcgaataat cgcttgctta tcaatataat tgccgaacat atccatagtg
      181 atatatatct aaccaagtat cagcaacaat atccagatgc caatgcatta ataaatactt
      241 tgcatgaatc tggcaatgat atggtgcagg atgaaaaaca tttgagtgat agcaaaaacc
      301 gttaatattt tcctttcatt gtgatcgtct gtaggcgatc acaatctatt tgtcttttat
      361 aacgtctaaa cttttttatg caaaggtgta tttatgattt tttttaacga tataaagccc
      421 actggttggt taaagaataa gatggaatgg tatatgaacg gtcatatagg agaactggat
      481 aaattagttc cccatcttat tacggagcat aaaatatatg gaagagatcg tataactcca
      541 cgcactgtat ctgctgaaga tggcgttatt aaaaaacaga ataatcccga tgataacatg
      601 atgcaatatt attggtggga tagtgaaact caatcgaatt ggaaggatgg atattgccgc
      661 agtgcctact tgttagataa taaagaatgg caggaaaaag cgagcgattt atgtttggaa
      721 cttatcgata cgcaagatga tgatggctac attggaattt atgattcatc attaaaattt
      781 aactgtaaag cagaaaacgg agagttctgg gcacaagcta ctgccttgcg ttttgtgctt
      841 ggatgttatg agtccacccg taatgagagt ttattaactt ctcttaccca agccgcacaa
      901 tgcattatgg ctggctaccc taaagaaacg tcacatcctt tcgttgtgga acagggtttt
      961 gctggacata gccacggatt aacaataaca gatgttttct accgccttta tgaaataacc
     1021 aataacattg aatatctctc atattgcttg tggctgtatg aagattattc cgcaggatgc
     1081 gtgtcggagc aagatctgca atataaaaat gtcatagacc ctggttacct tcttaaaggt
     1141 catggcgtac atacgtatga gcatattagg gcattagcca tcgcagcgag ccaacggccg
     1201 cagtatcaaa ctgcattaga tattttttta tcccagttga aatattatct tacaccatct
     1261 ggcgctccca ttggtgatga gtggattaca ggacgcacag ctaatgccac ttatacaggc
     1321 tatgaatatt gttctgttca tgaactattt agcacttatg ttatgttaat aaaactgacc
     1381 actaatattg gtctggccga tgatgctgag tggctattct ataatgctag ttttggcatg
     1441 cagcatccca tacttcacgc gataatgtac tgtaagactg ataattgtta tgaagcaaat
     1501 gaaaggaaac atcctgacag taatcagttt aataccagat ataaatactc ccctactcat
     1561 gaggaggctg ctgtctgctg cgtaccgaat acagcaagaa ccataccggc ttttgtcgaa
     1621 aatatgtttc aagaaaataa caatggtttt actgcattat tttatggccc gtgtgagttt
     1681 tatggcacat ttaataatgt tgcaattaaa attacacaat taactgaata ccctcatgat
     1741 ttaagcgtaa aatgtctcat tgagcctgag tcatctgttc gttttgctct cagtttccgt
     1801 taccccggct gggcaaaaat gatgattatt aatggtgtta cttttactaa aaatgatact
     1861 caaaactcac ttattatttt agatagaaca tggcaacatc atgatgtgat tgacattaaa
     1921 tttatagctg atatacagtt taatactgac ttatgtggtg acacttatat ttccagaggt
     1981 cccgttttat atgcaattga gcttgagagc gatattttaa tcaaaaaaaa cctgctgaac
     2041 gggaaatatt ttgactcagg atacataccc tgtagtagag ctgatgaatc tatccagttc
     2101 tcttatgctg atatggaatc tttttcatat tcaacctcac cagagggcaa acaatacatt
     2161 gaaggctgtt tttatttaaa caagactaaa atagtcagaa aacttatacc atttggtcaa
     2221 accattcttc gcaaagtaac tttctccaat atagagagtt aatattttcg agtgttataa
     2281 ttaattaaag ggatttctat gtaccttact gataatatta tcaaactgtc gaataaaata
     2341 ggtcagcgtg tttatactgt tgtctctgaa atgtcgattg aagcatggat aacaaaagaa
     2401 ccgcagcctt ttgtttatcg ccgtagcggc gcttaccaga aactcaccat tggctcagac
     2461 tggggtcaac tgtttgactg tgcctggatg cgagtttatg gtaaacgtcc agcagataaa
     2521 tttagacaca aactcgtcgc tctcgttgat atcggtggcg aaggtcttat tgtggataag
     2581 aatggttcac ccatctgcgg catcacgaat aaagcatcat cttatggcgt accgccagat
     2641 aaaccaggta aatgggtggt tgacctttct ctcgttagtg aaaatgacga ggttgagttt
     2701 tggattgatg ctgcctgcaa cgatttattt ggctatgtta ctaatggagg gattattact
     2761 gatgttcata tcgcgacacg taatcaacta ctgaaatccc tttattatga tattgaggtg
     2821 cttttcgact ggattaatga tggtcagaag tttgaatcca tacatcctaa aggtatcacc
     2881 ccagaacaat ttattgccaa aagaagtgag cgtactgacg aaatcataag tactctcgaa
     2941 tatattgata atacgttaat tacctttagc aatgaagaaa taattaaatg tcaaattgca
     3001 atccaatcca taatcaataa aaacaataat ccttcagagt tcaggattat ggctactggt
     3061 catgcccatt tagatatagc gtggatgtgg cctttaagag aaggacgcag aaaagcaata
     3121 agaacatttg ccaccgccct tgcaaacatt gaaaaatatc ctgattacat ttttggcgct
     3181 agtcagtacc aactttttca ctggataaaa aaagattatc cctatttctt cgaaaagtta
     3241 aaaaaacaaa tcgccgcagg ccgctttgag ttacaaggtt gcttctgggt cgaatgcgat
     3301 ttaaatctgg taagtggtga atcgctgata aggcaaatca tgcatgggac acgattcaca
     3361 ctgcagaatt tttcaaaaaa gataaattat gtctggcaac cggatgtctt tggattcccc
     3421 gcaacgcttc cacaaattct caa
//
LOCUS       DAZPJP010000301         3436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPJP010000301 DAZPJP010000000
VERSION     DAZPJP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.304"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(90..1592)
                     /gene="araA"
                     /locus_tag="ACHU9J_004238"
     CDS             complement(90..1592)
                     /gene="araA"
                     /locus_tag="ACHU9J_004238"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1907620.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(1603..3303)
                     /gene="araB"
                     /locus_tag="ACHU9J_004239"
     CDS             complement(1603..3303)
                     /gene="araB"
                     /locus_tag="ACHU9J_004239"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1907621.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
BASE COUNT          761 a          967 c          959 g          749 t
ORIGIN      
        1 tttgcttatc cggcctacaa aatcgcagcg tgtaggccag ataagacgcg tcagcgtcgc
       61 atcaggcgtt acatacctga tgtggctact tagcgacgaa atccgtaata cacttcgttc
      121 cagcgcagtg cgtctttaaa cgctggcagg cgggtgtcgt tatcaatcac cgtgatttca
      181 atgtcgtgca tctcggcgaa ctggcgcata tcgttgaggt tcagcgcatg gctgaagacg
      241 gtatggtgcg cgccaccagc gaggatccac gcttcggaag cagttggcag atccggttgc
      301 gctttccaca gcgcattcgc caccggcagt ttcggcaggg agtgcggtgt tttcaccgtg
      361 tcgatacagt taaccagcag acggtaacga tcgccgagat caatcaggct ggcgacaatc
      421 gctggaccgg tttgggtatt gaagatcagt cgggcaggat cgtccttacc accaataccg
      481 agatgctgaa cgtcgaggat cggtttctct tctacggcaa tcgacgggca gacttccagc
      541 atatgggagc cgagcaccag gtcattacct ttctcgaagt gataggtgta gtcctccata
      601 aaggaggtgc cgccctgcag accggttgac atcaccttca tgatgcgaag cagggcggcg
      661 gttttccagt cgccttcgcc cgcaaagccg taaccctgct gcatcagacg ctgtacggcc
      721 agacctggaa gctgtttcag accgtgcaaa tcttcaaagg tggtggtgaa cgcgtggaag
      781 ccaccttgtt ccaggaaacg cttcatcccc agctcaatac gcgccgcttc cagcacgttc
      841 tgtcgttttt cgccgtggat ttgtgtggca ggcgtcatgg tgtagcagct ttcgtactca
      901 tcgaccagcg cgttaacatc gccgtcgctg atggagttca ccacctgcac cagatcgcca
      961 accgcccagg tattgacgga gaaaccgaac ttgatctgtg cggcaacttt atcaccatcg
     1021 gtgaccgcta cttcacgcat gttatcgcca aaacggcaga ctttcagatg acgggtatcc
     1081 tgtttagaga ccgcctggcg catccaggag ccgatacgct catgggcttg tttatcctgc
     1141 cagtgaccgg taaccacggc atgttgctga cgcatacgcg cgccaatgaa gccgaactcg
     1201 cgaccgccat gtgcagtctg gttcaggttc ataaagtcca tatcgatact gtcccacggc
     1261 agcgccgcgt taaactgggt gtggaattgc agcaacggtt tgttgagcat ggtcaggccg
     1321 ttgatccaca ttttggccgg ggagaaggtg tgcagccaca ccaccagacc agcgcaacga
     1381 tcgtcgtaat tcgcgtcgcg gcaaatagcg gtgatttcat ccggcgtggt gcccagcggt
     1441 ttcaacacca gtttgcaggg cagtttcgct tccgtattca gcgcattaac aacgtgctcg
     1501 gcatgttggg tgacctgacg cagggtttcc gggccataca gatgctggct gccaatgaca
     1561 aaccacactt cataattatc aaaaatcgtc attatcgtgt ccttatagag tcgcaacggc
     1621 ctgggcagcc tgtgccgggg cggaagttgg aagatagtgt tgttcggcgc tcatcgccca
     1681 ttgctgatag cggcgataaa gctgttcaaa gcgttgtgcc tgttcgctgc gcggttgcag
     1741 ggttttctct accgcactgg ccattttttg ctgggctgat gggatgtctg cgtgcacttt
     1801 cgcggcgacg gcagcaaaaa tcgccgcacc gagcgcacag cactggtcag aggcaacaat
     1861 ttgcagcggg cgattcagca cgtcgcagca ggcctgcata atgacctggt ttttccgcgc
     1921 gatgccgccc agcgccatca cgttattgac ggcgatcccc tgatcggtaa agcactccat
     1981 gattgcgcgt gcgccaaagg cggtggcagc aatcaaaccg ccgaacagca gcggagcgtt
     2041 ggtagcgagg ttaagatcgg taatcacccc tttcagacgt tggttagcgt ttggcgtgcg
     2101 acgaccgtta aaccagtcga gcaccaccgg caggtgatcc agagacggat ttttggccca
     2161 tgcttcggtc agcgccggaa gcagttgttt ctggctggcg ttgatttgcg ctttcagttc
     2221 cggatgctgg gcggcaagct gttccagcgg ccagccgagt acgcgaccaa accaggcgta
     2281 gatatcacca aacgccgatt ggcctgcttc cagaccgata aatccaggca ccacgctgcc
     2341 atcaacctga ccgcaaatac ctttaactgc ccgctcgcca acgctctgtt tgtcggcaat
     2401 cagaatgtcg caggtggaag taccgataac ttttaccagt gcgttaggct gtgcgcctgc
     2461 gccaactgcg cccatatggc agtcaaacgc gccgccggaa atcaccacgc tttcaggcag
     2521 gccgagacgc tgcgcccatt ccgggcataa ggtgcccacc ggaatatcgg cagtccaggt
     2581 gtcagtgaac agcggggaag gcaaatggcg attgaggatc gggtccagct catcaaagaa
     2641 actggctggc ggcaagccgc cccagctttc gtgccacaga gatttatgcc cggcgctgca
     2701 acgtccgcga cgaatatcct gcgggcgggt ggtaccggaa agcagagctg gcacccagtc
     2761 gcacagctca atccacgatg cggcagattg cgccacggcg ctgtcctggc gagtcacatg
     2821 caggattttt gcccagaacc attcgctgga ataaataccg ccaatatagc gggagtagtc
     2881 aacgttgccc ggcgcgtggc acaaacgggt aatctcttcc gcttcttcaa ccgcagtgtg
     2941 gtctttccac aatacgaaca tcgcgtttgg gttttcggca aactctgggc gcagcgccag
     3001 cacgttaccg tcggcgtcaa tcggtgcggg cgtcgagccg gttgtgtcaa cgccaatccc
     3061 gaccacagct gcgcgctgtt cggcgctaag ctctgcaagc acgattttca gcgccgcttc
     3121 cattgactca atgtagtcac gcggatgatg gcggaactgg ttattcgggg catcacaaaa
     3181 ttgccctttc tgccaacggg gataccactc tacgctggtg gcgatctctt caccggtagc
     3241 gcattccacc gccaaagctc gcacagaatc actgccaaaa tcgaggccaa ttgcaatcgc
     3301 catcgtttca ctccatccaa aaaaacgggt atggagaaac agtagagagt tgcgaaaaaa
     3361 agcgtcaggt aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga
     3421 cgccgtgcaa ataatc
//
LOCUS       DAZPJP010000302         3410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPJP010000302 DAZPJP010000000
VERSION     DAZPJP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.305"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(123..323)
                     /gene="glgS"
                     /locus_tag="ACHU9J_004240"
     CDS             complement(123..323)
                     /gene="glgS"
                     /locus_tag="ACHU9J_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1907622.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            592..1221
                     /locus_tag="ACHU9J_004241"
     CDS             592..1221
                     /locus_tag="ACHU9J_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1907623.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1248..2909
                     /locus_tag="ACHU9J_004242"
     CDS             1248..2909
                     /locus_tag="ACHU9J_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1907624.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3148..3207
                     /locus_tag="ACHU9J_004243"
     CDS             3148..3207
                     /locus_tag="ACHU9J_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1907625.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT          963 a          790 c          794 g          863 t
ORIGIN      
        1 tttgatttgg cgattgatca gatcgcacaa tccgggctaa gttccctcaa agtgatctac
       61 tattccgcgc agctatttag catctaatac aaacactaaa acgggccatc aggcccgtta
      121 tatcagtgct ctaactccag ctctcttgcc tgcatcatct gttggcaata ccaggcataa
      181 cgtgcgcaaa accaggttct atgttcttca tccatgttac cagtaacggc aagaatatcg
      241 accgggcgac cttctgcgtg cattctggca aaactacgcg ccaggaaatc gaaattattt
      301 aaagaattaa gactatgatc cataagcaca tcctccggtt atcgcatacc ttttaaggcc
      361 atatgctttt tctgatttca gtattcccca atcagtgtag tgcctttaaa cataacgtgc
      421 gtaaatatat tgtccccgat cacacttttt agtgaaaaat aaaaaactat attaaaaaac
      481 tttctcttcc agaacttctc taacatgtgt tattattaac aaagataaat gtaagcaagg
      541 aaactatccc aaagtaaata ttgcgtcact aaatggacat tggagtgaca tatgatttta
      601 ttcgccgact ataatacccc ttatcttttc gctatttcct ttgtactgtt gatcggcctt
      661 ttagaaattt tagcgcttat ctgcggtcac atgctctccg gtgctcttga tgcacatctt
      721 gaccattata attccataac cacaggtcat attagccagg cacttcatta tctcaatatc
      781 ggaagattgc cagctctcgt cgttctctgc ttgctggctg gtttttttgg tcttattggc
      841 atcctgttgc agcatgcctg catcatggtc tggcaatcac cactgtcgaa cctgttcgtg
      901 gttcctgtca gtttactgtt tacgattatt gcggtgcatt ataccggtaa aatagttgcg
      961 ccctggattc cacgcgacca tagttcagct attacagaag aagaatatat tggtagcatg
     1021 gcattaatta ccggtcatca agccacttca ggtaatccct gcgaaggaaa actcaccgat
     1081 caatttggcc aaatacatta tttactactg gagcctgaag aaggaaaaat tttcactaaa
     1141 ggagtcaaag tgttaattat ttgccgactt tctgcaacgc gatatttggc ggaaaacaat
     1201 ccctggccac aaatattgta atttattaat caaaaggaaa tttaataatg gatgatattg
     1261 ttaattctgt gccctcctgg atgtttaccg cgattattgc cgtatgcatt ctgtttatta
     1321 ttggaattat tttcgccagg ctctatcgtc gcgcttcggc agagcaagct tttgttcgta
     1381 ctggtttagg tgggcaaaaa gtggtgatga gcggtggcgc aatcgtgatg ccgatctttc
     1441 atgaaataat ccccatcaat atgaatactc tgaagctgga agtcagccgc tcaaccattg
     1501 atagcctgat tacgaaagat cgtatgcgtg tcgatgtagt agtcgctttc tttgtacggg
     1561 taaaaccgtc tgtagaaggc atcgccactg cggcgcagac gttggggcaa cgcacgctat
     1621 caccagaaga cttacgtatg ttggttgaag ataaatttgt cgatgccctc cgtgcaacag
     1681 ctgcacaaat gaccatgcat gagttacagg atacccgcga gaacttcgtg cagggagtac
     1741 aaaatactgt cgcagaagac ctatcgaaaa acggtcttga gctggaaagc gtttcactta
     1801 ccaactttaa ccagacctcg aaagaacatt tcaatccgaa caatgccttt gacgccgaag
     1861 gtttaaccaa actgactcag gagacggagc gccgtcgtcg cgaacgtaac gaagttgaac
     1921 aggatgtaga agttgcggtg cgtgagaaaa accgtgatgc actttcgcgc aagttggaga
     1981 ttgaacaaca agaagcgttt atgacgcttg agcaggagca gcaggttaaa acccgtactg
     2041 ccgaacagaa tgcacgtatt gcggcttttg aagctgaacg tcgtcgtgaa gcagagcaga
     2101 cgcgaattct ggctgaacga caaattcagg aaacggagat cgagcgcgag caggccgtcc
     2161 gctcaagaaa ggttgaggct gaacgtgaag ttcgtattaa agagatcgaa cagcagcagg
     2221 tcaccgaaat cgccaaccag acgaaatcga tcgctattgc cgccaaatcg gaacaacagt
     2281 cccaggcaga agcgcgtgct aaccttgcac tcgcagaagc agtaagtgcg caacaaaacg
     2341 tagaaaccac tcgccagacc gcagaagccg atcgtgctaa acaagttgcc ctaatcgctg
     2401 ccgcgcagga tgcagaaacc aaagcggttg aactgaccgt gcgggcgaaa gcagaaaaag
     2461 aagccgcaga gatgcaggcg gcggctatcg ttgagttagc cgaagctaca cgtaaaaagg
     2521 gtctggcgga agcagaagca caacgtgcgc tgaacgatgc tatcaacgta ctttctgatg
     2581 aacaaaccag ccttaaattc aaactggcct tgttgcaggc gctacctgcg gtaatagaga
     2641 aatccgttga gccgatgaag tccattgacg gtatcaagat tattcaggtc gatggtctga
     2701 atcgtggcag cgctgcgggt gatgcaaaca cgggtaatgt ggggggcgga aacctagcgg
     2761 aacaagcatt atcagccgct ctctcttacc gcacacaggc accgctgatt gactccttgc
     2821 tcaatgaaat tggcgtttca ggcggctcac tggcggcatt gacttcaccc ttaacctcaa
     2881 caactcccgt cgccgaaaac gtagaataaa ttttcagcta tgaacgtccc tggcacaaaa
     2941 agccggggat tttttatatc tgcgttccgc taaaaactgc aaatgatcag gccgttgcaa
     3001 cgttttgcgt gaccgctcgg ggaaggcaaa actgcctctg ggaaagcgtt gcgcgaggtc
     3061 cggcgctcat caacaatcgg ggagtcagca aagggctgaa acgggaaagc ccctcccgag
     3121 gaaggggcct tacagaagga aagggttatg atgaagctcg tcatcatact gattgtgttg
     3181 ttactcgtaa gtttcgcagc ttattaacag ccaatcagag gggggagaaa tcctccctca
     3241 cccttgttcc tttactttag gttgaaaaaa aatcagcgtc aagaaggttt cattaacaca
     3301 tatccctggc ccaaaaagcc ggggattttt tatatctgcg ttccgctaaa aggtgcaaat
     3361 gctcaggacg ttgcagcgtt ttgcgtgatc gctcggggaa ggcaaaactg
//
LOCUS       DAZPJP010000303         3408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPJP010000303 DAZPJP010000000
VERSION     DAZPJP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.306"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHU9J_004244"
     CDS             complement(<1..1361)
                     /locus_tag="ACHU9J_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1907626.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHU9J_004245"
     CDS             complement(1426..1545)
                     /locus_tag="ACHU9J_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1907627.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHU9J_004246"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHU9J_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>3408)
                     /locus_tag="ACHU9J_004247"
     CDS             complement(2272..>3408)
                     /locus_tag="ACHU9J_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR1907628.1"
                     /translation="CLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKR
                     NEQTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQ
                     QVKGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDK
                     NGNCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLF
                     PEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFS
                     NYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLIT
                     GVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          938 a          772 c          617 g         1081 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaaca
//
LOCUS       DAZPJP010000304         3403 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPJP010000304 DAZPJP010000000
VERSION     DAZPJP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.307"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(30..290)
                     /gene="yfhL"
                     /locus_tag="ACHU9J_004248"
     CDS             complement(30..290)
                     /gene="yfhL"
                     /locus_tag="ACHU9J_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1907629.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            505..1695
                     /locus_tag="ACHU9J_004249"
                     /pseudo
     CDS             505..1695
                     /locus_tag="ACHU9J_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1880..3067)
                     /locus_tag="ACHU9J_004250"
     CDS             complement(1880..3067)
                     /locus_tag="ACHU9J_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1907630.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3067..>3403)
                     /locus_tag="ACHU9J_004251"
     CDS             complement(3067..>3403)
                     /locus_tag="ACHU9J_004251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907631.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          854 a          923 c          800 g          826 t
ORIGIN      
        1 aattaatact ctacttccag agtagaatat taaattttat ccgcgtggtg catcagcaca
       61 aatttatccc acaactgttc ttctgtctcg acatgcgccg gatctttcac aatagtattg
      121 gggatcgggc acaccttctg gcaggttggt gtctcgtagt gccctacgca ttcggtacac
      181 ttatcgctgt taatctcgta gatatgatct cccattgaaa tcgcctcatt cgggcattcg
      241 ggttcacaca tatcacaatt aatacagcgt ttagtaatta gtaaagacat ttcaatggat
      301 taccgttaaa tcattttaaa atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt
      361 aacttactgt atgttgatcc agtgtattta accttgataa actcagtcca gcaacacaaa
      421 accgcaacac attgcatttt gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg
      481 cgcctacgca gataaggatt gagaatgccg cgcactgtaa cacataatcc ggatagcccc
      541 aataatgacg atgttttagc cgcatctgaa aaatgggacg cctgtaaacc cccctatacc
      601 agcgcacaca tgaaaatctg tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg
      661 gctcgccgtt ccaaatacga aaaagagaac tatctccgta tcgatttcag caaagccggt
      721 aaggttacat tttacgccga gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc
      781 gagtggccgg agctcgctat ccagctggcg cgcgaaaaag cactaggtat ggctgacggt
      841 ggactgcggg cagagtccgt acatgcagcg ctggaaatgt accgggatga cctcaaagcc
      901 aaagtagccc ggcagaagct gagcccggac agtttcacaa cctacggggt gcgtatcgac
      961 cggattaaag caacgttcgg cgagcgtgag gtgttcagcg acgtaacata caatcggctg
     1021 gtggaagtgc tggacgagtg gatcgccact cgctcgaaca ataacgccct ggagttgttt
     1081 gccgagctcc gtcggttctg gaagttctgc gcacctactc tttgcaacgg ccgcaatgtt
     1141 gccgccagtc tgccagatga ttatgtttcc tcccgcgtac agaaacctac ccccacacgg
     1201 ctttttaccg atattgaatc aatcgcccga ctctggctca atgttgctgc ctgcacctct
     1261 gtacaccaga agaatgctgt tcgcttcatg atcatcactg gtgttcgtcc gattaatgtc
     1321 cataacctgc gctgggacta cgtttacgag gaggctggtg aaattgttta tccggaaggg
     1381 gttatcggca tgcgaggggc tatgaaaaca caaaaggctt tccgcctgcc gataacgcct
     1441 gagatccggc ggattatcga cgagcagaaa gcctggcgtg attcagttcc tgagtgcaac
     1501 agggattatg tatttttgca gccacgtgat ccaatgcagc cattttcaaa acgatcactg
     1561 gataagctgg tgaaaacata cagcccggac ggggctgtaa aaggaataaa acatgatggg
     1621 actgttaaag ggaaagacgg tgcatttaat acgatgtgcc gtaaattcct taagagcaat
     1681 gttattgcct tgatcgatag gaattaaaac cccaaaaaga ttaaaaaaac accacaaaac
     1741 ggatgtttct tcaacaccac ttttgctcat atgaacggaa ccgacgatta aactggatgg
     1801 ctctgattga ttcagggtat gaatggcggt tttttgctcc gtttccctca aaatggacgc
     1861 aactttccct ctgcggctct cagccgcacc accgcatccg ggccagcggc tcatgcatca
     1921 ggacctgctc tgccagacgg tagccccgct tcagccccgt aaaacgcatc tgactcccgc
     1981 acagcacgca cttcagcggg tcaaccttca gtaacctctg atacatccct ctccaggtga
     2041 tttgcatcgc cgtttttctc actgtctccg ttatgatgta caccacttct tccagtaacc
     2101 gccgtttcgc cggactcaaa aaaccgtagt acctcaccat acggaacccc ttatccgcca
     2161 catgccagga gaacctttcc atgaactcat ctccactcat caacaggtat tcttcccgtt
     2221 ttgttcggtg actgttgtaa cgcagaccga tttcatcctg accggcataa tgctccagac
     2281 gactcatcgg caccggtggc tttttcaggt aagagccaaa gtacaccgcc acatgggggg
     2341 cattatccat cacccgggat acgttgacat tccagccacg gcggtaatgc gtgtccagga
     2401 agcgattcca ttcccgttta ctgcttcctt ctgctgccag cgcatccggc atcaccaggt
     2461 cagggtattt ccgtgacagc aaccgtgtta tccggtagcg ccacatgctc atcaccttac
     2521 gggcgtaaaa atgaagattt ttccaggtgt ggcccgacgt cacaccaccg gcagttgtcg
     2581 ataaatggat atgcggatgc cactgctggt cacgccccca tgtgtggatc accgtgaata
     2641 tccccggctc cacatctgcc tgatggcaga tttccagtat cacatccgct gcaatgcggc
     2701 tcatctctgt cagtaaccac cggttgtgga acaccaggga ccagtactgg cagggaagtg
     2761 tgaacacaat atgctgccac gggcagtcgg ggaccaggct cagcagatac tgtatccact
     2821 gtgcgccagc cttcaccccg cagtgcgggc aggagcggct tttacaccgg aagcagacct
     2881 tttttgtatg gcaacagtcc ggtgatgaac agcaccactg tgtatacccc atcagtgtgg
     2941 tcccgcacgc catgattttg gtcaccgact caatcaccac cggacgtact gccctttccg
     3001 gctgcttctc cagccagtta agccagtggt ttccctgctg aaagatatcg gcaaaacggg
     3061 gaagcatcag aagggcgggg cgactccgtc cggccagtga accgtgccac actccgggca
     3121 gtacataccg ccggcgctga taccggaaag aatggtcgca aattcccgct ccgtgcagcg
     3181 ggcgatttcc ggataccctt cgtcatcaac acgtacaaac cagaagacca gctttttgtt
     3241 tcccgcatcc acaaagaacg gaatattcag gtctgcgcag cattcaacgg catcgtcaaa
     3301 actatcaaag cgcagaactt ctgcgtcttc ttcgtcaaaa aaatcatctt cgtgaagctt
     3361 cacgacatag cggggaagtt tgcttctttg agaggcgggt tta
//
LOCUS       DAZPJP010000305         3390 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPJP010000305 DAZPJP010000000
VERSION     DAZPJP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.308"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHU9J_004252"
     CDS             complement(<1..312)
                     /locus_tag="ACHU9J_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR1907632.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHU9J_004253"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHU9J_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1907633.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHU9J_004254"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHU9J_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1907634.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          920 a          880 c          737 g          853 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg
//
LOCUS       DAZPJP010000306         3380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPJP010000306 DAZPJP010000000
VERSION     DAZPJP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.309"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHU9J_004255"
     CDS             <1..185
                     /locus_tag="ACHU9J_004255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907635.1"
                     /translation="KKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACVQ
                     YLINQYMILQGGGNEE"
     gene            175..477
                     /locus_tag="ACHU9J_004256"
     CDS             175..477
                     /locus_tag="ACHU9J_004256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907636.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            532..1086
                     /locus_tag="ACHU9J_004257"
     CDS             532..1086
                     /locus_tag="ACHU9J_004257"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1907637.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            1208..1669
                     /locus_tag="ACHU9J_004258"
     CDS             1208..1669
                     /locus_tag="ACHU9J_004258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907638.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            2133..2876
                     /locus_tag="ACHU9J_004259"
     CDS             2133..2876
                     /locus_tag="ACHU9J_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1907639.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            2959..>3380
                     /locus_tag="ACHU9J_004260"
     CDS             2959..>3380
                     /locus_tag="ACHU9J_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907640.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHK"
BASE COUNT         1201 a          561 c          682 g          936 t
ORIGIN      
        1 tgaaaaaagt aactttaaag gaacgcaaac atcatgtagt aaccgcacgt cttaatgacg
       61 atcaagtact tgtacttaac cagctaatta gtagtgggaa ggtacagaaa gattcattat
      121 cagcctgtgt tcagtactta attaatcagt acatgatttt acagggcggt ggtaatgaag
      181 aatgactttg atttagtaca atattttgat gattacacaa tagtagggga tatggattat
      241 cactatttct attgtgtgaa gtatcaggaa ggttcattag aagcacatta tgaacgaatt
      301 ggtgatccag aagtattagg tactattaca tggaatggaa attacctaat cgacggatta
      361 ccaatggtaa tgtttgtact ggctgctgaa gatgaacata aggaatgttt aaaccgtttg
      421 ctaatcgtat gtgagaaggc gagggatgaa tataggctaa atgctactgg cctctaagta
      481 gtagcagacg cgatcacatg gagtgacgcg ataacaacaa ggacgtttga tatgaaccct
      541 aaaatcactt ggagattaat tccaaattca aaacattact acgtatctga aaatggcgat
      601 gtttacaatg ataaaacaaa acagtatata gctacgaaaa acccatctaa tggatacaaa
      661 aaagtttcta ttaatggcaa atcactatat gtacatgttt tggtttgtac tttattcaat
      721 ggtgctaaac ccattggagg aagatacact gtagatcata tcgaccgcga taaaatgaat
      781 aacaaagcaa aaaatttacg ttgggtaact cagaaggtta ataattccaa tagaaagcat
      841 aaaggtactc gtactcaaaa gcttactcta aagcaatggt taatgattgt tggagagtac
      901 cttaaaggac aattatctca atatgcaatc actaaatggt ttttaaatca aactggtatt
      961 aatatttgcc ctcagaatat aggtaagtta ttcaaaggta ctacctattc aaaatggtac
     1021 acaacatatc ttacgaataa gcaaagacaa agaatacaaa gcctaaccat tcaacgtaaa
     1081 ccataaaaaa tgccagtatt ccagtactgg catctattca aaactcaatg gcaatgaagc
     1141 caaacttatt aaatggcctg taatcactac gggtcacgat taattaattt atacaggatt
     1201 atttatcatg gcaaaaacct attcaacttt tgataacaga aacataaaag cactaattag
     1261 ccagtacaag ctatataaag atatcccatg cgaagacatc aagaacatca tgaaggactc
     1321 agtagctaaa cttaaagtga gagataagat tagtgtatat gatgtgtttg cagtattgag
     1381 taatgaagat gaattacgga atgatttact attcaatact atcaatgcta ttaaagtaca
     1441 acagaataag aaacaagtaa aagaacgtat gatcaggtac tatggggcag cgatcacgga
     1501 tgcagcaaaa cgaatagagc agtatatgtt ggcacaccca aacgcactgc cagcaccagt
     1561 atatggacaa agaacactaa catataaaga gtttggtaaa ctattggaac tacaaaaaca
     1621 aggtgacttt attgatgaca tgatcgagta cttaaagggc ttgcgttaat tgtaacttat
     1681 tacggcatat tacggcatgg atgtatgccg taatacggca aaaaaagccc ctgaaacccg
     1741 ctatttacca tcaatacggc aactaagtat agagatacga aatccattcc atttcattcc
     1801 atgtatttct ttctctatca gtattccaag aaactgtgac ttgcgtcacg aatactactc
     1861 gtcgtgaact cctcgtagta ttggttagta acgttgcgtt tttctcgcca gtactcgaaa
     1921 acccgcaagg cgttactact cgctttgctc gatagatgtt agtacagcaa atgaagtacc
     1981 taaagtgaat tggtaagtac gacatgcccc gccagttcca caactctcgc tctggtcatt
     2041 tccactacta tgatgctatt ggatagctac ataaagtact ggatcaagta ggcatttttt
     2101 gtgtacaaag agggaattaa gagagggata ccatgaacag aaaacaattc atccagtcgc
     2161 atggtgctac ttgtagtaac tggacttgga gttggtcatt tgtgaaccac gacaagaaaa
     2221 tggttatctt tggtgcatgg gatgtacaaa aagagcaaga aagatcagtc attctgaggg
     2281 agcggtggaa aaccaaagtt gtggagggaa aggaaagaaa aaatcttgga tatattcaag
     2341 ctattgaaca cattcaattg atagctgaag gttatgagct ttacacgttt gatatggagc
     2401 agggacgaaa cgatgagaat agagatgtag ctgttatcaa acggttcact ccaaaacttg
     2461 aaaaacgtta ccttcggaaa gaaggaacag tttggtatgc agacttttta cctaatccat
     2521 ttccagatga aattacctca cctgaaaact acgctgaggg tgcaaaaaag caagttacag
     2581 taaactccta cgagcgtgat ccaaaagcac gacaggcttg tattgaccat catggtactt
     2641 cctgcgggtg ttgtgagttt aactttgaga aagtctatgg tgaacacgcc aagggcttta
     2701 tacacgttca tcatatcaaa ccgctacaca cagtaggcga ggattacgag gtaaatccaa
     2761 ttactgatat ggttccactt tgtccaaact gtcacgcaat ggtacatcgc ggtaacgaag
     2821 tattaagtgt cgaaagttta aaagcactgt tgtttaaaga cagcaataaa atttgaattt
     2881 attttagact tgatacgaca tacttgaaaa gttatgaata caatatgctt taaattagac
     2941 atgtaagttg agagaaaaat gagcataaca acaatagcag ctacatataa agatgtgtta
     3001 actattgctt tttcggcgtt gtcatttatt attgcatgta cttccttata cttctccggt
     3061 agggcagcat ggcatgatag atcaaggcta aaaataacag ctgacctcgt ctacgagtct
     3121 tttgatgagg aaccttataa aatcgaggtg atagtgatca atgttggtag gcgtgatgca
     3181 gttttggaag ggatgctttg ccattatgaa aaaggcatta ggagccattc atctccaaaa
     3241 gagagcattg ttattaaaga aaagcaaaga aaaaaatttg aattggtaag ctcagactta
     3301 gtcacgatag ataatgaatg tgatatttgt catttagaag atataactat tttagatgtg
     3361 gaggggaatg aacataaaat
//
LOCUS       DAZPJP010000307         3364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPJP010000307 DAZPJP010000000
VERSION     DAZPJP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.310"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHU9J_004261"
     CDS             <1..784
                     /locus_tag="ACHU9J_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1907641.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHU9J_004262"
     CDS             781..1686
                     /locus_tag="ACHU9J_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1907642.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHU9J_004263"
     CDS             1683..2753
                     /locus_tag="ACHU9J_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR1907643.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHU9J_004264"
     CDS             2990..3343
                     /locus_tag="ACHU9J_004264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907644.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
BASE COUNT          888 a          774 c          930 g          772 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aaca
//
LOCUS       DAZPJP010000308         3323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPJP010000308 DAZPJP010000000
VERSION     DAZPJP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.311"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="ACHU9J_004265"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="ACHU9J_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1907645.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="ACHU9J_004266"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="ACHU9J_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR1907646.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="ACHU9J_004267"
     CDS             complement(1251..2825)
                     /locus_tag="ACHU9J_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR1907647.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3323
                     /locus_tag="ACHU9J_004268"
                     /pseudo
     CDS             3080..3323
                     /locus_tag="ACHU9J_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          851 a          790 c          714 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actgagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtgccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatgaa gac
//
LOCUS       DAZPJP010000309         3307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPJP010000309 DAZPJP010000000
VERSION     DAZPJP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.312"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHU9J_004269"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHU9J_004269"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1907648.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHU9J_004270"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHU9J_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1907649.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHU9J_004271"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHU9J_004271"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1907650.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHU9J_004272"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHU9J_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1907651.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          781 a          874 c          783 g          869 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaa
//
LOCUS       DAZPJP010000310         3272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPJP010000310 DAZPJP010000000
VERSION     DAZPJP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.313"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(271..346)
                     /locus_tag="ACHU9J_004273"
     tRNA            complement(271..346)
                     /locus_tag="ACHU9J_004273"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(311..313),aa:Asn,seq:gtt)
     gene            complement(447..1934)
                     /gene="yeeO"
                     /locus_tag="ACHU9J_004274"
     CDS             complement(447..1934)
                     /gene="yeeO"
                     /locus_tag="ACHU9J_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1907652.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2095..2170
                     /locus_tag="ACHU9J_004275"
     tRNA            2095..2170
                     /locus_tag="ACHU9J_004275"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2128..2130,aa:Asn,seq:gtt)
     gene            complement(2208..3158)
                     /gene="cbl"
                     /locus_tag="ACHU9J_004276"
     CDS             complement(2208..3158)
                     /gene="cbl"
                     /locus_tag="ACHU9J_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1907653.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          958 a          855 c          690 g          769 t
ORIGIN      
        1 tttcccgata aagacgcgat agcgacatcc cgcataaggc atttttctct ttatctttgt
       61 acggtacttc atggaacaga gtttttgacc ttgcgaatcg tgatgtctgt tggggagggg
      121 caatttgctc actgaagcgt gagactcgat taagcgcacg aaacacagaa atcaaaaaac
      181 ccggtcactt ttttacaagg taaccgggta aaaataattt ttatttttta actgttttga
      241 gactcataga gatgtctcaa aactaaaatt tggctcctct gactggactc gaaccagtga
      301 catacggatt aacagtccgc cgttctaccg actgaactac agaggaatcg tgagaacgag
      361 gcgaatatta gcgatgccca cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt
      421 tgctgaaata atctgcattt tgtcgtttat tccgacacaa ctggcttttt ttcacacttt
      481 tgcggttcgg atcgagggta tttccacagc caacgtccag taaccattcg ccagtaaaac
      541 agcacggccc gcacagccca gtcggcaaac attcccatcc agacaccaac cacaccccag
      601 ccaagcatga tgcccagcac ataaccgacg acaacccggc aaccccacat gctcaacata
      661 gaaacccaca tggcgtaacg ggcatcgcga gcacctttaa atccagcggg tagcacccat
      721 gaggcggacc aaataggcat aaataaagca tttagccaaa tcagaatcac aacgacatgt
      781 ttaacctgtg gatcctgggt gtaaaacgat gccataaccc cggcaaaggg agccgttagc
      841 caggcgatgg ccgttaatcc aagagtggaa agccagaaca catgccgcaa ctgaatctct
      901 gcttgcgcta tctgccctac ccccaacctt cggcctgtaa tgatcgtaga agcagagccg
      961 agcgcacttc cgggtaagtt gataagagcc gcaattgaaa acgcgataaa atttccggca
     1021 ataacactgg tccccatccc ggcaacgaac atttgggtta ataaccgacc actggtaaat
     1081 aacactgatt cgacactcgc gggaatacca atccccatga cttcccagat aatgctaaaa
     1141 ttcagcggtt taaaatagct ctttaacgaa atccttagcg caggattaaa accaatcgcc
     1201 agcacccaca aaattgcaac tgcgccaata taacgagaaa tggttaaacc cagccctgcc
     1261 ccgacaaatc ccagtcccgg ccaggagaaa aggccgtaaa tcaatatgcc gctaataata
     1321 atattaagaa tattcaggct accgttaatc aatagcggta ttttcgtatt ccctgcacca
     1381 cgaagtgccc cgctaccaat aagagtgatg gcagctgctg gataactgag taccgtcagc
     1441 tccagataag tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca
     1501 ataatttgtt cgccaaaatg atgaataagc gttgccaaca gtacggcaaa caacgtcatg
     1561 atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta
     1621 aatgccacaa cgacagtagt accaagatcg atagcagcaa aaaaagccat aatgaccata
     1681 ttgaagctgt ccgccaatcc cacgccggcc atcgcatctt ttcccagcca gctgaccaga
     1741 aaagtgctca gaacccccat caacaggaca caggcattct ccatgaagat aggaacagca
     1801 agcggggtta tctcgcgcca gaacaacact ttgtagctct tgcgtttagc gtgccagcga
     1861 gtgccgtgaa caacctggcg taaagcagag gagatattca aagccgacct taattgcaga
     1921 aagtgaaacc acatttcaaa taatgaggga gaatcagcaa agctgcaaag attttcgcca
     1981 acaaattgtc tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt ggggaattta
     2041 aaaatcgggt ttgacaaaag atttttcgcc gttaagatgt gcctcaacag cgattcctct
     2101 gtagttcagt cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc
     2161 agaggagcca aatttctgaa aagcccgctt ttatagcggg ctttttgcta tatctgataa
     2221 tcaatttcct cttcactgtt ttccatcacc tgccgcttga tatcctcaac tgacagtcct
     2281 gcattacaaa gttccagaaa gcgccagaca tagttacgct gaagttgtcc tcgcttcagt
     2341 cccaaccaga cagtattagc atcgaaaaga tgccgcgtat ccagacggat taaattctct
     2401 tcctcttgtt cgccgctgga ttgttcggca actaatccga tcccaagccc aagagcaaca
     2461 taggttttaa tgacatcaga atcctgcgca cttaatacaa tatctgccag caaacctttg
     2521 cgggcaaatg cgtcatcaat acgtgagcgc cccgtaatcc cctgtcggta agtgattaac
     2581 ggccacttcg ctattgattc cagcgtcaat ggtgtaattt gcgttaaggg atgatcgagt
     2641 ggaacaagca aactatggtg ccaacgaaac cacgggaagg cgacgagctg cgggtcatta
     2701 ctcaaacgct cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt
     2761 tcctgtggcg tcccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct
     2821 ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaacg
     2881 ccagacgtat cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattcaga
     2941 atacgttctg caatgaccag taatgctttg cccggttcag tcatgcccag cagtcgctta
     3001 cctcgtcgaa caaatatttc gatgccaagt tcatcctcca gttcccgaat atgacggctg
     3061 acgccggact gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt
     3121 gcagcctcgc ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga
     3181 tttcccgtta ttgttaaagt ctaatgcccg gcataacaaa taataaaaac ccgcatctta
     3241 ttccatcccg atataacgct tagctcacca at
//
LOCUS       DAZPJP010000311         3260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPJP010000311 DAZPJP010000000
VERSION     DAZPJP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.314"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            143..859
                     /gene="nanC"
                     /locus_tag="ACHU9J_004277"
     CDS             143..859
                     /gene="nanC"
                     /locus_tag="ACHU9J_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1907654.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            879..1985
                     /gene="nanM"
                     /locus_tag="ACHU9J_004278"
     CDS             879..1985
                     /gene="nanM"
                     /locus_tag="ACHU9J_004278"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1907655.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2050..3030
                     /locus_tag="ACHU9J_004279"
     CDS             2050..3030
                     /locus_tag="ACHU9J_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1907656.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3140..3260
                     /locus_tag="ACHU9J_004280"
                     /pseudo
     CDS             3140..3260
                     /locus_tag="ACHU9J_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          967 a          691 c          765 g          837 t
ORIGIN      
        1 ggagacctta tacaaaaata taaaataagc tacccgttgc caaagacact ataagcctgg
       61 caaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
      121 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
      181 gtgcttttcg tcaccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
      241 tagcggaagc catgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
      301 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
      361 cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt aaacttgatg atcaatggac
      421 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
      481 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
      541 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
      601 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
      661 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
      721 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
      781 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
      841 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
      901 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
      961 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     1021 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     1081 ctgcattccc tggtggaccg agagatcaag caacctctgc atttattgat ggcaatctat
     1141 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     1201 acaaatacaa ccccaaaacc aatagctggg ttaaattgat gtcgctcgcg ccgatgggca
     1261 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     1321 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     1381 tagataaaat caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt
     1441 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     1501 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     1561 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     1621 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     1681 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     1741 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     1801 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     1861 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     1921 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     1981 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     2041 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     2101 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     2161 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     2221 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     2281 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     2341 catattgcac ggaaattact gcccttcatt cctaataatg cagggattct tatcgttccg
     2401 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     2461 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     2521 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     2581 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     2641 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     2701 actgacgcgc catggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     2761 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     2821 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     2881 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     2941 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     3001 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     3061 gttcgttctc agagtagaca acaaaaaaca aataagatca aggtaatatc tcttcacttg
     3121 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     3181 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     3241 cttttcgatt atggctcgga
//
LOCUS       DAZPJP010000312         3229 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPJP010000312 DAZPJP010000000
VERSION     DAZPJP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.315"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHU9J_004281"
     CDS             <1..495
                     /locus_tag="ACHU9J_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1907657.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHU9J_004282"
     CDS             525..3041
                     /locus_tag="ACHU9J_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1907658.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3229
                     /locus_tag="ACHU9J_004283"
     CDS             3052..>3229
                     /locus_tag="ACHU9J_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1907659.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTRL"
BASE COUNT          941 a          773 c          734 g          781 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggagttta ctagactgg
//
LOCUS       DAZPJP010000313         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPJP010000313 DAZPJP010000000
VERSION     DAZPJP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.316"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHU9J_004284"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHU9J_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1907660.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHU9J_004285"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHU9J_004285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1907661.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHU9J_004286"
     CDS             complement(1715..1984)
                     /locus_tag="ACHU9J_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1907662.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHU9J_004287"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHU9J_004287"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1907663.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHU9J_004288"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHU9J_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1907664.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPJP010000314         3162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPJP010000314 DAZPJP010000000
VERSION     DAZPJP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.317"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(34..288)
                     /locus_tag="ACHU9J_004289"
     CDS             complement(34..288)
                     /locus_tag="ACHU9J_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR1907665.1"
                     /translation="MPEAISGRLLVLVINTHCERKLFTSKNSNDPEKIARRFCTPDNC
                     LVVVLRNNRFLFHLERAPGHASRWYKGRTSICRRLYDWLT"
     gene            complement(452..1135)
                     /locus_tag="ACHU9J_004290"
     CDS             complement(452..1135)
                     /locus_tag="ACHU9J_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR1907666.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLSEDVWGAK
                     QEQTAITLH"
     gene            complement(1389..1922)
                     /locus_tag="ACHU9J_004291"
     CDS             complement(1389..1922)
                     /locus_tag="ACHU9J_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR1907667.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIQPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            complement(2364..2651)
                     /locus_tag="ACHU9J_004292"
     CDS             complement(2364..2651)
                     /locus_tag="ACHU9J_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1907668.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            complement(2689..2847)
                     /locus_tag="ACHU9J_004293"
                     /pseudo
     CDS             complement(2689..2847)
                     /locus_tag="ACHU9J_004293"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
BASE COUNT          846 a          720 c          768 g          828 t
ORIGIN      
        1 ttcttccctt cgttggttga tacaggattt gtatcaggtc agccagtcat acagacgacg
       61 acagatggat gttcgccctt tgtaccagcg actggcgtgg ccgggagccc gttccagatg
      121 aaacagaaag cggttattgc gaaggactac caccaggcag ttgtcagggg tacagaaacg
      181 acgggcaatt ttctcgggat cattgctgtt ttttgacgta aatagtttcc gctcacaatg
      241 ggtgttgatg accagaacca atagtcgtcc agaaattgct tcaggcattt gtgactcctg
      301 tatacgtaat ggcagtggtg cagctgtacc gtcatgaccg cacgatcccg gttgcgagaa
      361 atggcacaag cgattagatg aaaagaaaaa tgaccgggaa tggcagaaat ggcagtacag
      421 actgcggatt aggatgaata ctgtacctgt ctcagtgtag ggtgatagcc gtctgctcct
      481 gtttggcacc ccagacatct tctgacaagc tgggtaaccc gataaacaga gtatgaaaga
      541 gagtttctct gcgttcgatc ccgaattgat gttcgtatcc tctcaccacc tgaaatcctt
      601 cgtcggagaa tattaactct gccgtttcca gaagctgcat agttttgcgg tgtagtggag
      661 tgatcagctg caacgtaact ggtgccctca tatcgcctgt tggtagaaaa cactcttcgg
      721 tgcgacttag ttgtagcaga tgacgtttcc cggaacaggc ccgttcaatg gattcattga
      781 acaccctctc aggccatggg cagacatatg tgcgccagcc tgctttctgt aatttaccca
      841 gtgcagcccg aaaacgaaac aacggacatt ccccgacagt ggcagagctg agaaagagcg
      901 tattcagcgt tccgatgatg ggaatattgc gatctttttc ggtcaatgct tttaacgcca
      961 tgatttcgcc agggagacta cggaactcag gatgtctgct ggcaccagaa agccattcaa
     1021 gcaggtaatg ccgtaatccg gtttcactgg ttatattgtg gttttcctgt gccgttttca
     1081 gggcattcag tgcacaccag agcaactggc gcacgggatg ttctgtcgtg gtcatgcgaa
     1141 tgtcctactg tgagatgaaa agaagaccgg tatccgttct ctccgccaca cgtgatggca
     1201 cgggccggcc tttgataaaa cgaatcaata ctctggggtg aagcctttta tttctttcgc
     1261 cattaagaaa tacacggttc catcaaaaga cagggggagt ccatggtggg gatggctccg
     1321 tgcaggcttc actccagaat attctcttga attgaaaaaa gcccggcccg aaggccggga
     1381 aatactggct atgggatgtt tacaaggcgc tccataaagc cgtgatgttc ggcatttgat
     1441 accagttcca ggagcaggga tatcctcgac tcaggctgga gagtattttc cattctgagg
     1501 agatactgat attgtgcttt tgtcagccat atgctggaag ccgcattcag ctcttccctg
     1561 gctcccggat ttagcacagg gtccgggtaa atcttcatta atccatcaac aatatcttta
     1621 ttgacgggtt ttatttcacc gccaaacttc acgaaatcaa tgaaggcgct atggcgacgt
     1681 aatgccgata ctggttgaat gtcaaaatta gccagaataa aaaagtaccc tggaaaaata
     1741 gcgaatattc tttcacggta acagcacctg gagtctgccc gtcttatctt tcttgttatt
     1801 aatggagtcc atggtacgac gttttgctca ttcagccatg agaataaaga ttctctgttt
     1861 ttgccggctg tattatattg cgcaagatac cagttccggt tattcaggtg ttctatattc
     1921 acgaagttca acgctccgcg caaaaccgat ccatgatgga cgattttgcg atgtcaggcg
     1981 aatatgctaa atcatatgtc tgaagcagta tccagcccgt atctggagaa actgcagttc
     2041 tgacaggcat ataaccagca gactctagta cgttctgtat cggtgatttg gcgaactcac
     2101 agataaagaa cggctaagca ctaatatcag caatacagtg gcacagtatt gtgatacgcg
     2161 aaataaaaca tcagcacatc agttcagagt aaggtcgcca tatatcgggc atgagccaga
     2221 tacaaataac tgggacaccg tctgaagtga tttgctttat taaagtaact tgtgcttggt
     2281 gtttatgttt atcaatttgg gatcgcctgt aaagtgcaaa atgcaatttc catgaaagtg
     2341 tcccctttaa tggggacacc tgttcacatt ttttcgtttt ctttagctgt attgctaatc
     2401 atgaaaacga aagttcgcat cagcacagca gagcgaagtg catcatatcc ttccagattt
     2461 aaattcttca ggcttcgctt caggtaggtt cgtaccgttt cgggagaaag ccgacagttc
     2521 tgggcaatgg cgtcgtaagg aacacccatt gcgtaaaaaa cgcagacttt aagctggttt
     2581 gttgatagct cgggaaacat gccttccaac agagtgatgg ccgggagttt ctgtatcatt
     2641 tgaggtgaca tagcaaaggt ctccgatgac aactaaaaac gctatcaccg agttcctacg
     2701 ctcattggtg atagcccaga cgggggtagg aataccggcg tcatcggaaa ccggccaggc
     2761 taatgcctgc cccgcctgag ctaccataga tgacgacaag aaatacaagt tcctgtcttt
     2821 atggtatggc aaaaaacgac gccaaatgaa gaaattaact tctgcgccga cgatgactac
     2881 gcgggttcct acgcccgatc acagttttgc ctgtgaccag taaagggtac ttagtataac
     2941 gtaccgagtc aactaacagc ttaaaaaaca tacaaaaaag cgtcaaaact taacgctccg
     3001 cgccgaagcg atccaggcat gatgcttctg ctttgtggcg ttcgaacatg ctggtccata
     3061 tgcacgaaac ggtgtctgac cctcgtccag acaatccgca gttctgacga acatatgaac
     3121 aaatatgctc ttgtagaggc tgttgttaca caattggtgg gt
//
LOCUS       DAZPJP010000315         3154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPJP010000315 DAZPJP010000000
VERSION     DAZPJP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.318"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..148
                     /gene="ynaL"
                     /locus_tag="ACHU9J_004294"
     CDS             <1..148
                     /gene="ynaL"
                     /locus_tag="ACHU9J_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1907669.1"
                     /translation="PVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKLSHRERRSAR
                     IRAC"
     gene            178..1551
                     /gene="dbpA"
                     /locus_tag="ACHU9J_004295"
     CDS             178..1551
                     /gene="dbpA"
                     /locus_tag="ACHU9J_004295"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1907670.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(1680..2615)
                     /gene="ttcA"
                     /locus_tag="ACHU9J_004296"
     CDS             complement(1680..2615)
                     /gene="ttcA"
                     /locus_tag="ACHU9J_004296"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1907671.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2667..>3154)
                     /locus_tag="ACHU9J_004297"
     CDS             complement(2667..>3154)
                     /locus_tag="ACHU9J_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1907672.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
BASE COUNT          728 a          794 c          797 g          835 t
ORIGIN      
        1 tcctgtccct gaaccgattc ctggcgatcc tgttccagtg cccgatccga tccctcgccc
       61 gcaacccatg cctgacccac cacccgatga agaaccgatt aaattgtcgc atcgtgagcg
      121 tagatctgcg aggatacgcg cctgctaact ttgcgtcgat gaccacgaga atagattgtg
      181 accgcttttt ctaccctgaa tgttttgcct cccgcccaac tcacgaacct taatgagttg
      241 ggttatttaa ctatgacgcc ggtgcaggcc gccgcgcttc cggcgatcct tgccggaaaa
      301 gatgttcgcg tgcaggcgaa aaccggcagc ggcaaaacgg cggcttttgg cctcggcttg
      361 ttacagcaaa ttgatgcgtc gctatttcaa acccaggctt tagtgctgtg tcctacgcgt
      421 gaactggcgg atcaggtggc aggtgaattg cgtcggctgg cgcgttttct gccaaatacc
      481 aaaattttga cgttgtgcgg tggtcaaccg ttcggtatgc agcgtgattc gttgcaacat
      541 gcgccgcata ttatcgtggc aacgccgggt cgtttgctgg atcacttgca aaaaggcacg
      601 gtatcactgg atgcgctgaa tactttggtg atggatgagg ccgaccgcat gctggatatg
      661 ggatttagcg atgccattga tgatgtcatc cgttttgcgc ctgcatctcg acagacgctt
      721 ctgttttcgg caacctggcc ggaagccatt gccgtaatca gcggacgagt gcaacgcgat
      781 cctttggcga ttgaaattga ctcaacagat gctttgccac ccattgaaca acaattttat
      841 gagacatcca gcaaaggcaa aattcctctg ttgcaacggt tattaagctt gcatcagcca
      901 tcctcttgcg tggtgttttg caataccaaa aaagattgcc aggctgtctg cgacgcgctg
      961 aatgaagtag ggcaaagtgc attgtcatta cacggcgatt tggagcaacg cgatcgcgat
     1021 cagaccctgg tacgttttgc taacggtagc gcccgtgtac tggttgcgac tgatgttgct
     1081 gcgcgtggtc tggatattaa atcgcttgag ctggtggtga actttgagct ggcgtgggac
     1141 cctgaagttc atgtacatcg catcggccgt acagctcgtg caggaaatag cggtctggcg
     1201 atcagtttct gtgctccgga agaagcacag cgggccaata tcatttctga catgttgcag
     1261 ataaaactta actggcaaac gccgccagct aatagttcca ttgtgccgct ggaagcagaa
     1321 atggcaacgt tgtgtatcga tggtgggaaa aaagccaaaa tgcgcccggg tgatgtatta
     1381 ggtgcactga caggagatat cgggcttgat ggcgcagata ttggcaaaat cgccgtgcat
     1441 ccggcgcatg tctatgtcgc ggtccgtcag gctgttgctc ataaagcatg gaaacagtta
     1501 cagggcggga agattaaagg aaaaacgagc cgggtgcggt tattaaaata atgaaatgtt
     1561 gaattgccgg gtgcaagagt aaacatctta ttcgggattg ccggatgcga cgctggccgc
     1621 gtcttatccg gcctccataa gagtagcccg atacgcttgc gcatcgggcg ccatcctggt
     1681 tatttcactt caaccacatt cagccgtaac tcatccaact gattttcatc ttcttctggc
     1741 tgccagcccg ccggttgtag tgggatctct tcgcgatcaa acgccagatc acccccgtta
     1801 accacttcag aaccgtgggt gatgcctttg aaatcgaaca ggttggtatc gcacagatgc
     1861 gacggcacca cattctgcat cgcgctgaac atcgtctcga tacgccctgg ataacgttta
     1921 tcccagtcac gcaacatgtc agcaatcacc tgacgttgca ggttaggctg tgaaccgcac
     1981 aggttgcacg gaataatcgg gaacgctttt gcatcggcaa atcgctgaat atctttctcg
     2041 cggcagtagg ccagcggacg aataacgata tgtttgccat catcgctcat cagtttcgga
     2101 ggcatacctt tcatcttgcc gccgtagaac atatttaaga acagcgtctg caggatatcg
     2161 tcacgatggt gacccagcgc gatcttcgtc gcccccagtt ccgttgcggt acgataaagg
     2221 ataccgcgac gaaggcgaga acacagtgag caagtggttt tgccctctgg aatcttctct
     2281 ttcacgatac cgtaagtgtt ctcttcgaca atcttgtact caacgcccag cttttcaaga
     2341 tactcgggca gaacgtgttc cgggaagccc ggttgctttt gatcgaggtt aacagccacc
     2401 agcgaaaaat tgattggcgc gctttgctgc aaattgcgca gaatctccag catggtatag
     2461 ctgtctttac ccccggagag gcaaaccatg atgcgatcgc cttcttcaat catattgaag
     2521 tcagcaatgg cttcgcccac gttacgacgc agacgttttt gtaatttgtt caggttgtat
     2581 tgttctttct ttgtaatttg ttgattttct tgcattattt cagttctctg gtactaaatg
     2641 gggcaaattg ggggcaaact ttgcaactac gataaccgcg cattcaacat ggctatctgt
     2701 tcgtcgttca tgtcatcaat ccacataccg taaatttcat acaccatttg tgcagtttca
     2761 tgccccatct ggctggctat aaatgccggg ttcgctcctg ccgtcaacag ccagcaggca
     2821 aaagtatgtc gcgtatggta cggattacgg cggcgaatac cagcacgttt tactgctgca
     2881 ttccatcttg cacccaaact gcttaccgag taataaggtt tctgtttttc gttacacacc
     2941 ctgggcatga aaacaaaatg cagtttttgc ttttcagttc tgccgtactc ccgatgataa
     3001 aaagtgattt cgcttttgcg atgatgcccg gtcagtttgt attgctcctt cagtgcttca
     3061 agagccggct gcagtaatgt taccgtccgg atcccggcat ttgtttttgg gggaccgaac
     3121 atatcaagta tcgtcaggtt tcttctgaca ttca
//
LOCUS       DAZPJP010000316         3131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPJP010000316 DAZPJP010000000
VERSION     DAZPJP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.319"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHU9J_004298"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHU9J_004298"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1907673.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHU9J_004299"
     CDS             complement(1496..3121)
                     /locus_tag="ACHU9J_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1907674.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          892 a          722 c          727 g          790 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc c
//
LOCUS       DAZPJP010000317         3045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPJP010000317 DAZPJP010000000
VERSION     DAZPJP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.320"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHU9J_004300"
     CDS             <1..172
                     /locus_tag="ACHU9J_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907675.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHU9J_004301"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHU9J_004301"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR1907676.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHU9J_004302"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHU9J_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHU9J_004303"
     CDS             2190..>3045
                     /locus_tag="ACHU9J_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR1907677.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZPJP010000318         3034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPJP010000318 DAZPJP010000000
VERSION     DAZPJP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.321"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            386..799
                     /locus_tag="ACHU9J_004304"
     CDS             386..799
                     /locus_tag="ACHU9J_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907678.1"
                     /translation="MFFIENEGQAVARTDYWRSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            801..1580
                     /locus_tag="ACHU9J_004305"
     CDS             801..1580
                     /locus_tag="ACHU9J_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1907679.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1761..2405
                     /locus_tag="ACHU9J_004306"
     CDS             1761..2405
                     /locus_tag="ACHU9J_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR1907680.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2492..2800
                     /locus_tag="ACHU9J_004307"
     CDS             2492..2800
                     /locus_tag="ACHU9J_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFR1907681.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
BASE COUNT          746 a          713 c          787 g          788 t
ORIGIN      
        1 cggtttccgg cggttcagga tcttcctctc cccccacaca acgccacctc ccgtcatcac
       61 aacatgtggt gccggattca gctgctgatg acactatatg ttgtgtcatc tccctgacct
      121 gtgatgcgtc gcgcaggggc ggaaaacagc gatatgatta tttcctcagc gtggtacact
      181 tccggaaagt tgtgatattc gagaaagtcg gatctgacgg aaacggctct ccggtaattt
      241 aacggcgtgg ttatatggat gcttgttatc atggtgatga tgataacggc atgatgttat
      301 cagacggcgt gacggtaagg gcagtgatga tggatgacgt tatcgcatga ccgtccctgc
      361 ccggaaaaga aaaaaggagt cacccatgtt ttttattgag aatgaaggtc aggctgtcgc
      421 cagaacggat tactggcggt ctgtacaggc gcaggccgga tatgtctacc tcagctggaa
      481 tgccggcgca gccaggctgc ttgtcccgga tgcggcaaaa catttactca gggagatgcg
      541 gggggctgag tacgtcatca tcagtaaggg aacgctgcat ggccgcgatg cgctggaact
      601 ggtatttgaa gacggcagcg atgcgccgtt tgtgatccac atgctgagtg agcagtgcga
      661 tcgcctgctc cccgaaaaca accagggcgg gggttttgtt gtcaccgtct ggacgcgtgg
      721 cggtaaccag ctccgttatc cgggaaagta ccgtgttgtg gaaaacctgc ccgacgtttc
      781 cccgtggagt gaacactgat atgcagcacc tgccggcacc gatccaccat gcccgggatg
      841 ccgctcagct tcctgttgcc atcgattatc cggcagcgct ggcactccgc cagatgtcga
      901 tggttcatga tgaactgcca aaatacctgc tggcccctga agtgagcgcc ctgctccatt
      961 acgtcccgga tctgcgccgc aagatgctgc tggccactct gtggaacacc ggcgcgcgta
     1021 ttaatgaagc actggcgctg acgcgggggg atttttcgct cacgcccccg tatccgtttg
     1081 tgcagctggc cactctgaag cagcggaccg aaaaagccgc caggacggca ggaagaatgc
     1141 ccgccggtca gcagactcac cggctggttc cgctctccga cgcctggtac gtcagccagc
     1201 tgcagacgat ggtggccaca ctgaaaatcc ccatggaacg gcgtaataaa cgaacaggca
     1261 ggacagagaa agcgcggatc tgggaagtga cggacagaac ggtcaggacc tggattgggg
     1321 aggcggttgc cgccgctgcc gctgatggtg tgacgttctc tgtcccggtc acgccacata
     1381 cgttccgcca ttcctatgcg atgcatatgc tgtatgccgg tataccgctg aaggtcctgc
     1441 agagtctgat ggggcataag tccatcagct caacggaggt ctacacgaag gtgtttgcac
     1501 tggatgtggc tgcacggcac cgggtgcagt tttcgatgcc tgagtccgat gcggtgtcta
     1561 tgctcaaaag aattccataa gctctgactt ttttaaatcc ctttcggata tctcatggat
     1621 atattatcgg tatttcatat tctgttgaca tgcaggaccg gtatagagta aaaaaacatc
     1681 tattgagtac ctcttggata tccgaaaggt gtcttatggg gggctttatg ggtattacat
     1741 gtggattctg gagatacact atgcctgtta ttgctaatac gcatcccaaa ggtggtgtgg
     1801 gtaagacaac ttcatccgta aacattgttg gtgaaatgaa gtctgataca gttgatctgg
     1861 atactcatac cgggctttct atcattctgg ggctgagacc tgaaggaaaa gaaatttccg
     1921 tgaaagtacc caaaacagtg gatgagttaa tcgaaattat gactccctac aagaacagcg
     1981 ataagacgtt acttattgac tgtgggggat ttgactcaga tcttacccgt actgcgattg
     2041 cctttgcaga ctgcgttatt gtcccttcaa aagactccct gactgaacgt attggtttga
     2101 tgcattttga tggagtactg gacgaaatca gctctatcat ggggaccgat ataactgctc
     2161 acctgtatct ctgtaaagtt aatcctaaca agaagaaatt ccctaagctt gatgcaatcc
     2221 tgccatcgtt caaacatctg aagctgatga agagccgcat ttctgctcgt gcagaatttg
     2281 aggatgtcat tgagactggg atgggaatca ctgagtctgt tcatgggcgc tattcggcag
     2341 gaggtaagga agttattgcc ctgatcgaag aaatcaatca tctgattgaa aataacaaac
     2401 agtaaggtgt gtctattggg tatccatata gtatcctttg ggcttttaat ggatatcttt
     2461 taagtatcta acaaacatcc tgggttatct catgagcaaa gcgaaattta gtgcacaggc
     2521 tgaaaaactg aaaaacctgg tggattctgc cagattacaa acttcttctt ctgccgtaac
     2581 agcaacagcc ccgtctcagc caaatgaagg tgcagaggaa gtttcaggtc gtcccgtggc
     2641 tgccaggacg aaggctaaga atcttaaggc cataccgctg tcgtattttg aagctcatgc
     2701 ggaacttaaa gcgaccagta aaacatctct ggattttagc tcatatatta ttgaggctat
     2761 aagagagaag cttgaacgtg atggtgcaat aggtcaataa actatcggat accttaaggg
     2821 tatctgatgt gtgtctttta aatgcgttat ggggattctt cacgtaccta aagagtcccc
     2881 attagacatc tttctgttta tggtgacatg attcgtcacc gctttccttt cctctcagtt
     2941 tcaccatttc attccttttc ccctaacctg aaccatgatg tccacgatgt ttttatattg
     3001 catttatgta tcgcatataa ccttcataat gttt
//
LOCUS       DAZPJP010000319         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPJP010000319 DAZPJP010000000
VERSION     DAZPJP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.322"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHU9J_004308"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHU9J_004308"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1907682.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHU9J_004309"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHU9J_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR1907683.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHU9J_004310"
     CDS             2805..>2992
                     /locus_tag="ACHU9J_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR1907684.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPJP010000320         2987 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPJP010000320 DAZPJP010000000
VERSION     DAZPJP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.323"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(126..202)
                     /locus_tag="ACHU9J_004311"
     tRNA            complement(126..202)
                     /locus_tag="ACHU9J_004311"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Asp,seq:gtc)
     gene            complement(335..1066)
                     /gene="dnaQ"
                     /locus_tag="ACHU9J_004312"
     CDS             complement(335..1066)
                     /gene="dnaQ"
                     /locus_tag="ACHU9J_004312"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1907685.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1131..1598
                     /gene="rnhA"
                     /locus_tag="ACHU9J_004313"
     CDS             1131..1598
                     /gene="rnhA"
                     /locus_tag="ACHU9J_004313"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1907686.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1595..2317)
                     /locus_tag="ACHU9J_004314"
     CDS             complement(1595..2317)
                     /locus_tag="ACHU9J_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1907687.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2351..>2987
                     /gene="gloB"
                     /locus_tag="ACHU9J_004315"
     CDS             2351..>2987
                     /gene="gloB"
                     /locus_tag="ACHU9J_004315"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1907688.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQ"
BASE COUNT          813 a          726 c          697 g          751 t
ORIGIN      
        1 atatgtgatt aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt
       61 aaatgcaaaa aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt
      121 gaatttggcg gaacggacgg gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc
      181 taaccgactg aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga
      241 ttgcctcacc cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc
      301 ccaagccgct atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc
      361 ccttcttctc caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa
      421 aaacaacgcg taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt
      481 gctgctgtgt ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca
      541 gataaacttc cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt
      601 tatctatttc gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt
      661 tcctcgccac cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc
      721 gcttaagcaa cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca
      781 ccaactccgc gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct
      841 tatcgagcaa aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc
      901 gatcgggttt gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa
      961 cggcaccaat ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg
     1021 tggtttcggt atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg
     1081 tcagacttat cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac
     1141 aggtagaaat tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg
     1201 ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca
     1261 acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg
     1321 aagtcatttt gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata
     1381 actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct
     1441 ggcaacgtct tgatgctgcc ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc
     1501 atgccggaca cccggaaaac gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc
     1561 ccacactgga agatacgggc taccaagtcg aagtttaagc ttgtggttta cgacattgcc
     1621 gggtggctcc aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta
     1681 gcgttaaagg aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa
     1741 tatgcgcatt caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg
     1801 cgtgcagcac ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca
     1861 tccggctgtt atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta
     1921 aactaatggg attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg
     1981 tttcacgcaa taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa
     2041 catcaacgga tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca
     2101 ttccttgcgc agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg
     2161 cgcttaaatt gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga
     2221 gctggcgctc cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag
     2281 gagccacgac agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactaa
     2341 gaggtaatct atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt
     2401 gaatgatgaa gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa
     2461 cgccattgcc gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga
     2521 tcacgttggc ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc
     2581 acaagagaca caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt
     2641 tttggggcat gaatttagtg taattgccac accgggtcac actttaggac atatctgtta
     2701 cttcagtaaa ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtaggtt
     2761 gtttgaaggg acagcatcac aaatgtatca atcacttaat aagttaagtg cgttacctga
     2821 cgatacattg gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag
     2881 tattcttccg cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc
     2941 aaaaaatcaa ataacactac ccgtaattct gaaaaatgag cggcaaa
//
LOCUS       DAZPJP010000321         2931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPJP010000321 DAZPJP010000000
VERSION     DAZPJP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.324"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            260..433
                     /locus_tag="ACHU9J_004316"
                     /pseudo
     CDS             260..433
                     /locus_tag="ACHU9J_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1162..2829
                     /gene="ltrA"
                     /locus_tag="ACHU9J_004317"
     CDS             1162..2829
                     /gene="ltrA"
                     /locus_tag="ACHU9J_004317"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFR1907689.1"
                     /translation="MNTHISVSTIPHPTGWHTINWKACHARVRKLQLRIAKATRQQQW
                     RQVRELQRILTRSFSGKAVAVRRVTENTGKRTPGIDGKIWHTPKEKWEGICSLNLCGY
                     RPQPLRRIYIPKSNGKTRPLGIPTMRDRAMQALWLLALEPVSETTADHNSYGFRPMRS
                     THDAIESIFLRMSQKVSPKWILEGDIKGCFDNISHDWLLSHIPLDRKLLKKWLKAGYM
                     ERGVFNHTNSGTPQGGIISPVLANMALDGLEKELMQTFRKSGYHSAKHQVNYVRYADD
                     FICSGSSRELLENEVRPLIAAFMRERGLELSEEKTAITHIDKGFDFLGQNVRKYNGKM
                     LIKPSKKNLKNFLCKVREIIKRNPTLPAWKLIGQLNPVIRGWATYHRHVVAKETFNYV
                     DTQIWRAIWRWCVRRHPRKGLRWIAGRYFSFEGRRWIFKAITPEGKILTLFRAMETPI
                     KRHIKIKGEATPYTPGMEIYFERRLDLIWKGKSKKMKTVVQLWKRQGKHCPQCGQPIT
                     NQTGWNIHHRIRKVMGGSDELTNLELLHPNCHRQLHSREAGAHRKHL"
BASE COUNT          799 a          756 c          837 g          539 t
ORIGIN      
        1 ttgagtttcg ggagccgcgg ttttgacgtt ttgcggggac ctgctctttg ttccctccgg
       61 tattcaccat cacctgtttg agaaccttcg ccgcatgggg catcactgcc acggcctgac
      121 gcacctgtcc tgggcagaac gcaaaatttt ttaccgggtt ttgggtaaaa aattttgtgg
      181 gctgttcatt taccctggac agtcagtgcg gcaggttgtg gctgtatcag cccccggcga
      241 caggggctta aacaggtgaa tggcaccgga cacaacaacg ggcagggacc cgccgcaacg
      301 acgtgaagcg cggtttgcga aacggcgttg cgccttacat cccggcggcc ttttgccggg
      361 atgccaggag cgcaggccac ggcgcgacag aggaggcgcg accggaacgg tcgctgcaag
      421 cccggggttt tcacgcatcg gaccgtagcg gcagcccgaa accgcgccag cgggttcggc
      481 ggagactggc aggtacgcca gcagctgcct gtcggagtcg ggcctgacga ccggatgccg
      541 ccaggcagcc gggagccgaa ggtgcgcgtg cgacaggaag tcgcttaacg tattgtttta
      601 cattgataaa accagctatc ccattagctg tagcctaccg ggacaggcgc tgccggcaac
      661 ggcggggtct gaagctcccg ggacaacgag agccgggcgt cagcccgggt aaagccccgt
      721 gaggggcttt accttgctgc aagcatcatg cggcaggaga gcaaggctgg tcagaagggt
      781 taacacatgt gaactgttga taaacaccgt tatcggcaac aggccaaaga tgctgacagg
      841 cctgaaccaa aaggtacggg gtccggttgc tacctcgcgc acagggagca catgggcgca
      901 gagccccccg gtgaagagac agaacccgcc ctgaccctct gtatgttaag cggaacctgg
      961 taaacccgta tcgctgcctc acaggcaggt gaaccgcaag gaacactgtt agcggtgcgg
     1021 gcagaggagg acggaaaaag cgaatgcccg gctgtaataa ccgggatacg gggcaggttc
     1081 cccgacgcga aagcgggcag acttccgtct ggtctttaat tgcatgaaaa gctgtaaagc
     1141 agtcaaaagg atatcagcga gatgaataca cacatcagtg tgtccacgat accccacccg
     1201 acaggctggc acaccataaa ctggaaagca tgccatgccc gggtgcgaaa gctacagtta
     1261 cggattgcaa aggcaacccg acagcaacaa tggcggcagg tcagagaact acagcgcatc
     1321 ctcacccgct cattctccgg caaagccgtg gctgtcaggc gtgtaactga aaataccggt
     1381 aaaaggacac ccggtattga cggaaaaata tggcacacac cgaaggagaa atgggaaggg
     1441 atatgcagcc tgaatctgtg tggttacagg ccacagccac tacggcgaat atatatccca
     1501 aaatcaaacg gcaaaacgcg accgctgggc atacccacaa tgagggaccg ggccatgcag
     1561 gcactgtggc tgctggctct ggaacccgta tccgagacca ctgctgacca caacagctat
     1621 ggtttcagac caatgcgcag cacgcatgac gccattgagt ccatttttct gcggatgagc
     1681 cagaaagtat cgcccaaatg gatactggag ggcgatatta aagggtgttt cgataacatc
     1741 agccatgact ggctgctttc ccatattccg ctggacagga aacttcttaa aaaatggttg
     1801 aaagcaggtt acatggaaag aggcgtgttt aaccatacga acagcgggac accgcaggga
     1861 ggcatcatat cccctgttct cgccaacatg gctctggacg gactggagaa ggaactgatg
     1921 caaacgttca ggaaaagtgg ttatcacagt gcaaagcacc aggtgaatta tgtgagatat
     1981 gctgatgatt tcatctgttc aggcagctcc cgtgaattgc tggaaaatga ggtcagacca
     2041 ctgattgcag ctttcatgcg cgaacgaggg ctggaactgt cggaagaaaa aaccgcgatc
     2101 acacatatag ataaaggctt cgacttttta gggcagaacg tcaggaagta caacggtaaa
     2161 atgctgatta aaccgtcgaa gaaaaacctg aagaacttcc tctgtaaagt cagagaaata
     2221 attaaacgca atccgacgct cccggcatgg aaactgatag gccagctaaa cccggtgatc
     2281 cggggatggg ctacctatca ccgacacgtc gtggcgaaag aaacatttaa ttatgtcgac
     2341 acccaaatct ggcgagctat atggcgatgg tgtgtaagaa ggcacccccg aaaaggattg
     2401 cggtggatcg ccggacgata cttctccttt gaaggcaggc gctggatctt taaagccata
     2461 accccggaag ggaaaattct gactctcttc cgggcaatgg aaacaccgat aaagcgccat
     2521 ataaaaatca aaggcgaagc aacaccatac acccccggaa tggaaatcta ctttgaacgt
     2581 cgtctggatc taatctggaa aggcaaatca aagaagatga agacagtagt tcaactctgg
     2641 aagaggcagg ggaaacactg tccgcagtgc ggacaaccca taaccaacca gaccggatgg
     2701 aatatacatc accggatcag gaaagttatg ggcggcagtg acgaactgac caatctcgag
     2761 ttgttacatc cgaactgcca ccgacagtta cacagcaggg aagccggtgc tcaccgaaag
     2821 cacttataaa ggcttgagcg gagtgcgggg aaactcgcac gctccgttct tagggggcgg
     2881 cggcgcagca atgtgtcgct gctacccgac ccggcagaca gaacaacact g
//
LOCUS       DAZPJP010000322         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPJP010000322 DAZPJP010000000
VERSION     DAZPJP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.325"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHU9J_004318"
     CDS             <1..493
                     /locus_tag="ACHU9J_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1907690.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHU9J_004319"
     CDS             493..1083
                     /locus_tag="ACHU9J_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1907691.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHU9J_004320"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHU9J_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHU9J_004321"
     CDS             2170..2658
                     /locus_tag="ACHU9J_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1907692.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHU9J_004322"
     CDS             2682..>2917
                     /locus_tag="ACHU9J_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1907693.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          759 a          636 c          831 g          691 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctaccag gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZPJP010000323         2899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPJP010000323 DAZPJP010000000
VERSION     DAZPJP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.326"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1198)
                     /locus_tag="ACHU9J_004323"
     CDS             complement(<1..1198)
                     /locus_tag="ACHU9J_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1907694.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNS"
     gene            1491..1585
                     /locus_tag="ACHU9J_004324"
     tRNA            1491..1585
                     /locus_tag="ACHU9J_004324"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1525..1527,aa:Sec,seq:tca)
     gene            1857..2899
                     /locus_tag="ACHU9J_004325"
                     /pseudo
     CDS             1857..2899
                     /locus_tag="ACHU9J_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          664 g          699 t
ORIGIN      
        1 cgctgttctg cgctttttct gccgcatcat atccgccata agccagcatc cagccgataa
       61 gagcgccgcc gaaggccaac cccagtttga gcacaaacag cgtgccagca aaactgatcc
      121 cggtcaggcg tttaccattg caccactcgc cgtagtcgac ggtatcggac atcattaccc
      181 actggatagg tgtcaccagt tgatgcaaca caccaatcac gaagatgaag acaaacatag
      241 tgatgctggc ctgcatggga acaaagaaca tcgcgaggct aatcactgcc agcagggcgt
      301 tcgtccacca gaagatagtg actttacatt tccagtcggt cagaggtttt gccagtgcgg
      361 aaccaatcag gttacccacg caataagtgg tgagaaaagc gacaaacact tccggcgtgc
      421 ccaaaatcca tgtgacgtaa tacatcatcg ccccaccgcg tacgcacacc gccaggatat
      481 tgaaaatggt tagtaaaccg acaatccgcc actggtcgtt ttgccagata tcacgtaaat
      541 cttcccgcat agacgttgtt gtaggtggtg cttcaacgcg ttctttagtg gtgaagaaac
      601 aaaatgccag catcatgaat gccaccacgg aaaggaccgc gataccgccc tggaaaccga
      661 gtggtttatt atcaccgcca attaaattaa ccagtggcat catcagaaca gtagaaagca
      721 tgcctcccgc ggtcgccagc acaaaacgcc aggattgcag cgagatacgc tgagtcgggt
      781 cattggtgat tacaccaccc aatgcgcagt aagggatatt gacgacggta taaagtaagg
      841 taagtagggt gtaagtaatt gctgcataga tcattttgcc gttcatactg agatctggcg
      901 tgctataggc cagtacacag acgatcccga atggcagtgc gccaaacagt acccacggac
      961 gaaatttacc ccagcgagag cgcgttcgat cggccaacaa ccccatgcaa ggatcggaaa
     1021 tcgcatccag tgcacgagcg accaaaaaca tggttccgac aaatccggca ggaatgccaa
     1081 aaatatcggt ataaaagaac atcatatata acattacgtt atcgaaaata atgtggctgg
     1141 cggcgtctcc catgccataa ccaattttct ctttaacgga caacacttca ctcttcatct
     1201 gcttttcctt gatgctatag gggtgctgag accgataacg cattttgtaa accatccctg
     1261 tgagagcgaa tattccgata tctggttatt aatttatgat tcttgtttta tgtgatcgtg
     1321 gtagcgttaa ttccgctcat atatcattgt aaaatatggg ttttatatga gttataatgc
     1381 tttcgtgata atacgctgcg tgtattaggc ggaaaaaact gatctggggg atgtagaaac
     1441 tcaaggaagt agctataatg cgccccgcct ccatgtagca atcgaggcgc ggaagatcgt
     1501 cgtctccggt gaggcggctg gacttcaaat ccagttgggg ccgccagcgg tcccgggcag
     1561 gttcgactcc tgtgatcttc cgccagttta cctcttctga actctcccaa tgtcaataaa
     1621 tctcttctaa tcccttgtat atcagtattt atttgtatcc tgaggtcaac ctacttcacc
     1681 ccatatcaag tttcttttgg gggtacttta gggggtgtta tttcctattg aaatacaagc
     1741 acccccataa taggaaaaat gtgatacata acaacggctt ttaacaatat ctttgttata
     1801 caaggggtgt attgggggta tcaggttagt tactggcaga aatccccctc aggaacatga
     1861 attcatcaaa agccggagga tgtcatggca ttaaccgata tcaaagtgaa aaccgcaaaa
     1921 cccaaagaca aaccctataa gcttgctgat ggcggcggta tgtatctact gattaacact
     1981 aatggttcta aatactggcg catgaagtac cgctttgctg gtaaagaaaa gatgctttct
     2041 attggcgtat accccgatgt aacgctagcc gatgctcgcg agaaacgcag tgaagccagg
     2101 aaactcctcg ctgctggagg cgatccaggg gaggcaaaaa aggaagaaaa aatcgcgcag
     2161 caaatgagtc tgaaaaatac gttcgaggct attgctcgcg aatggcacca gtcaaaagct
     2221 gatcggtggt cattacgcta tcgtgatgaa atcattgata cctttgaaaa agacattttt
     2281 ccctacattg gcaagcgccc tattgccgaa atcaaaccaa tggagttact ggaagcactg
     2341 cgtaaaatgg aaaaacgtgg agctctggag aaaatgcgca aggtccgcca gcgctgtggt
     2401 gaggtgttcc gctatgcaat tgttactggc cgggcagact ataacccagc ccccgatctt
     2461 gccagtgctt tagctacacc gaaaaaagta cattttccct ttcttactgc taatgaactt
     2521 ccccacttcc tcaacgatct ggcgggttat accggaagta taatcactaa gacagctact
     2581 cagatcatta tgttaaccgg cgtacgaaca caagaactgc gttttgcacg ctgggaagat
     2641 atcgattttg agacaaaatt gtgggagatc ccggcagaag taatgaagat gaagcgacct
     2701 catattgtgc cgctctctga gcaggtcatt atgctattta agcagcttga accaatctca
     2761 aaacatcatc ccctagtctt tatcggtaga aatgatcccc gcaaaccaat tagtaaggaa
     2821 agcattaacc aagtaattga attacttgga tataagggaa gactcacagg gcacggtttc
     2881 agacacacca tgagtacaa
//
LOCUS       DAZPJP010000324         2880 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPJP010000324 DAZPJP010000000
VERSION     DAZPJP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.327"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHU9J_004326"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHU9J_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1907695.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHU9J_004327"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHU9J_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1907696.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHU9J_004328"
     CDS             1650..2099
                     /locus_tag="ACHU9J_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1907697.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHU9J_004329"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHU9J_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1907698.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHU9J_004330"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHU9J_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1907699.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2880
                     /gene="yncL"
                     /locus_tag="ACHU9J_004331"
     CDS             2787..>2880
                     /gene="yncL"
                     /locus_tag="ACHU9J_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1907700.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          759 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttattt
//
LOCUS       DAZPJP010000325         2856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPJP010000325 DAZPJP010000000
VERSION     DAZPJP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.328"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            68..400
                     /gene="hdeA"
                     /locus_tag="ACHU9J_004332"
     CDS             68..400
                     /gene="hdeA"
                     /locus_tag="ACHU9J_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1907701.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            516..842
                     /gene="hdeB"
                     /locus_tag="ACHU9J_004333"
     CDS             516..842
                     /gene="hdeB"
                     /locus_tag="ACHU9J_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1907702.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            906..1553
                     /locus_tag="ACHU9J_004334"
     CDS             906..1553
                     /locus_tag="ACHU9J_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR1907703.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1595..2125)
                     /locus_tag="ACHU9J_004335"
     CDS             complement(1595..2125)
                     /locus_tag="ACHU9J_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1907704.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHU9J_004336"
     CDS             complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHU9J_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1907705.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          829 a          568 c          641 g          818 t
ORIGIN      
        1 aggttataac ctcagtgtcg aaattgattc gtgacggctc tttcacttta tagttgagga
       61 tattacgatg aaaaaagtat taggcgttat tcttggtggt ctgcttcttc tgccagttgt
      121 gagcaatgca gcggatgcgc aaaaagcagc tgataacaaa aaaccggtca actcctggac
      181 ctgtgaagat ttcctggctg tggacgaatc cttccagcca actgcagttg gttttgctga
      241 agcgctgaac aacaaagata aaccagaaga tgcggtttta gatgttcagg gtattgcaac
      301 cgtaacccca gctatcgttc aggcttgtac tcaggataaa caagccaact ttaaagataa
      361 agttaaaggc gaatgggaca aaattaagaa agatatgtaa ttccgggaat gcgttacatc
      421 gtacttcctt gcatattgaa caggccggaa tatcttcttt aaaagcagct attcctcctg
      481 ttcatatata atctctatat tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag
      541 cgtttatttt tatgggcgct gtagcggctt tgtcactggt gaacgcacaa tctgcgctgg
      601 cagccaatga atccgctaaa gatatgacct gccaggaatt tattgatctg aatccaaaag
      661 caatgacccc ggttgcatgg tggatgctgc atgaagaaac agtatataaa ggtggcgata
      721 ccgttacttt aaatgaaacc gatctcactc aaattcctaa agtgatcgaa tactgtaaga
      781 aaaacccgca gaaaaatttg tataccttca aaaatcaagc atctaatgac ttgccgaatt
      841 aatgaggtac aagtaaaaag gagtagcaag ttgagccatc ttgctgctcc tttttgcatt
      901 tttatatgac agcagaattt attatacgtc ttatactcgc ggcaattgcc tgtggcgcta
      961 ttggcatgga aaggcaaatg cgcggcaaag gagcagggtt acgcacacat gtattaattg
     1021 gcatgggaag cgccctgttt atgattgttt cgaaatatgg ttttgctgac gtgctgtctt
     1081 tagatcacgt cggactcgac cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt
     1141 ttatcggtgc aggtaacatt ctcgttcgta accaaaatat tgtaggtctg acgacggcag
     1201 cggatatctg ggtgaccgcc gccataggta tggttattgg cagcggtatg tacgaactgg
     1261 gtatttatgg ttcagtgatg accttgttgg tgctggaagt cttccatcaa ttaaccttcc
     1321 gcctgatgaa caaaaattac catttacagc tgacattagt gaacggtaat atggtatcaa
     1381 tgctcgactg gtttaagcag caaaaaataa aaactgattt agtttcgttg caggaaaatg
     1441 aagatcatga agtggtcgcc attgatatcc agcttcatgc cacaacgtcg atagaagacc
     1501 tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt ttcaattagc taatctgtgt
     1561 ggcagcgtag ccagactcac cgtaagcctg aaattcacac cagataatca atatgctgat
     1621 ggcgtaccag ctcattgatc cgcttaagat tgaggatcat catgatattg tgtttatggc
     1681 agtagaacgt ttttagcgat attttcagtt tctccagaat atcttcagtt gtcatttgat
     1741 tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag tgacacattc gcttcaggca
     1801 cagagcagta actgccattg atgagtgcga caaaatcaat gatagtgata gtccgcggaa
     1861 caaaggtcac cggtactttc cccagacagc gtttaatatt aaagggggag aaaacaatga
     1921 catggacagg ttttaatcgt tcaatttgag tgagaaaatt agaatgaaat acattattca
     1981 ttaatgtatc aataatgacg aaggcattct gatgcaacat tacgtcgatc tcttcttcgt
     2041 tctgtatatg aacgacatta cctttactca gaatgttttt catcgcggtg aagaacatcg
     2101 tatccctggt aattataaga aacataaccg actcctggtg cggacattaa ctttataatc
     2161 aatttgccaa aggtaattaa caggacaggg cttataataa taatagttag cttactgtat
     2221 attaatttta tgtaatacat gatgcagatg ctgagaaagc atctgcatct ttcggtggtg
     2281 ttatttgacc agctcaggtg ttacctgact caccgcattg gtgtagtaag gcgcacccca
     2341 gcccggttcc ggccagaatg caccatagcc gtaatcccac aggttatagg tggtattaac
     2401 cacttctctc aaatgccaca cctggatgcc ctgcatattc acttccagga agttatacgg
     2461 gactttattg ataaagccag gttgttcacc ctgaatggta ccgaggatgg taacaaagtg
     2521 attacgatag ttcactgggt caaggaagcc gctttgtctg gcgagcagtc ggccctgata
     2581 gttggcttca atatcaggtt tcgcatagct atccaacggt aatacagcga tttctaacaa
     2641 cgtatccgtt ttgccattga taacgttgat aaccttccca ccaaagcgcg cttgttgacc
     2701 aacatataac cccggctggt tatgaacagc aacaaaactt ttttgaatat caggttggtt
     2761 attgcctttg atattttgcg gaattgaact acatgcggca agcaaaaatg aaaggctgag
     2821 gatgagtgca ccttttgtca tgttcatgtt actatc
//
LOCUS       DAZPJP010000326         2850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPJP010000326 DAZPJP010000000
VERSION     DAZPJP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.329"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            113..2392
                     /gene="ydeP"
                     /locus_tag="ACHU9J_004337"
     CDS             113..2392
                     /gene="ydeP"
                     /locus_tag="ACHU9J_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1907706.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2640..2837
                     /gene="safA"
                     /locus_tag="ACHU9J_004338"
     CDS             2640..2837
                     /gene="safA"
                     /locus_tag="ACHU9J_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1907707.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          765 a          663 c          689 g          733 t
ORIGIN      
        1 agagagtagt cccctactct tatatatcca tgttggcgat aatccctttg tatgtactgt
       61 gcatcatcgc tattacaaat cctaataatt catttccaca caggataagt agatgaagaa
      121 aaaaattgaa tcctaccagg gtgctgcagg tggttggggt gctgttaaat ccgtagcgaa
      181 tgcagtacgt aagcagatgg atatacgcca tgatgttatt gccatgtttg acatgaataa
      241 gccagagggc tttgactgtc cgggttgtgc atggccagat cctaagcaca gtgcgtcatt
      301 cgacatttgt gaaaacggcg caaaagcaat cgcctgggaa gtcacggata agcaggtaaa
      361 tgcctctttc tttgctgaga atacggttca atcattactt acctggggag atcacgagct
      421 tgaggctgca gggcgtctca ctcagccttt gaaatatgat gccgtcagcg actgttacaa
      481 gccattaagc tggcaacaag ctttcgacga aattggcgca cgccttcaaa gctatagtga
      541 tcccaatcag gttgaattct atacttcggg ccgcacttcc aatgaagctg cctttcttta
      601 tcagcttttt gcccgtgaat acgggagcaa taactttccc gactgctcca acatgtgcca
      661 tgaaccgaca agcgtgggtt tggcagcgag tatcggtgta ggtaaaggga ccgtgttgct
      721 ggaagacttt gagaagtgcg atttagtcat ttgcattggg cataaccctg gtacaaacca
      781 ccctcgcatg ctgacttcgt tgcgcgcttt agtgaaacgg ggagcgaaaa tgatcgccat
      841 caatcctcta caggaacgtg gcctggagcg atttaccgca ccgcaaaacc cgtttgaaat
      901 gctgacgaac tctgagactc agttggccag tgcctactac aacgtgcgca ttggtggcga
      961 tatggcgttg ctcaagggga tgatgcgcct gttaattgag cgcgatgatg ctgcaagtgc
     1021 cgcaggtcgg ccctcattgc ttgatgacga atttattcaa acgcataccg tcggctttga
     1081 cgagctacgc cgtgacgttc tcaattccga gtggaaagat atcgaacgta tttctggact
     1141 aagtcagaca caaatcgccg aactggctga cgcatatgcc gctgccgatc gcaccattat
     1201 ctgttacgga atggggatca ctcagcacga acatggtacc cagaatgtac agcaactggt
     1261 caatctgctg ttgatgaaag gtaacattgg caagcctggt gcgggtatct gcccactacg
     1321 aggtcactct aatgtacagg gcgaccgaac cgtcggtatc accgagaaac cgtctgcaga
     1381 gtttctggct cgcctgggtg agcgctatgg cttcacccca cctcatgcac ctggacatgc
     1441 tgcaattgcc agcatgcaag caatatgcac ggggcaggct cgagcattga tctgcatggg
     1501 gggcaacttt gcactggcaa tgccagatcg ggaagcgagc gctgtaccgt taacgcaatt
     1561 agatttggcg gtacacgtag ccactaagct taaccgctct catctgctga ccgcacggca
     1621 tagctatatt ctgccggttc tggggcgtag cgagattgac atgcaaaaaa gcggtgcgca
     1681 ggcggtaacc gttgaggatt caatgtcgat gattcatgcc tcgcgtggcg tgttaaaacc
     1741 cgccggtgta atgctgaaat cagagtgtgc agtggtcgcg ggaatcgcgc aggcagcact
     1801 accccacagc gtggtagcct gggagtatct ggtggaagat tatgatcgca ttcgcaatga
     1861 cattgaagct gtgttaccag agttcgccga ctataaccag cgcatccgac atcctggagg
     1921 ttttcacctg ataaatgcag ctgctgaaag gcgctggatg acgccgtcag gtaaggctaa
     1981 tttcattacc agcaaagggc tgttagaaga tccctcttca gcgtttaaca gtaagctggt
     2041 catggcgaca gtacgtagcc acgatcagta caacacgacg atttatggta tggatgatcg
     2101 ctatcgaggg gtattcggtc aacgagatgt ggtctttatg agtgctaaac aagctaaaat
     2161 ttgccgtgta aaaaacggcg aaagagttaa tcttattgcg cttacgccag acggtaagcg
     2221 cagctcacgc cgcatggata gattaaaagt ggtcatttac cctatggctg accgctcact
     2281 ggtgacctat tttccagaat cgaatcacat gctaactctt gataaccacg atccattaag
     2341 tggcattcct ggttataaaa gtattccggt tgaattagaa ccatcaaatt aattcgcctc
     2401 ctcatttctt ctgctgtcat ccgcacagca gaagaatttc tcattgacta ttatcgcgca
     2461 atttgctcac attgattaaa ttaaaccaca cactataaga tataaacctc tgcctacagc
     2521 tgtaagaaac tccgctcagt actgaagtgc cagtcctatt tcctcttttc tccagcctgt
     2581 tatattaagc atactgatta acgattttta acgttatccg ctaaataaac atatttgaaa
     2641 tgtatgcgac cacagtgaaa aacaaaatca cgcaaagaga caactataaa gaaatcatgt
     2701 ctgtaattgt ggttgtctta ttactgaccc ttacgttgat agccattttt tcggcaattg
     2761 atcagctggg tatttcagaa atgggtcgca ttgcaagaga tcttacacat ttcattatca
     2821 atagtttgca agactgaaaa taaacagcaa
//
LOCUS       DAZPJP010000327         2717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPJP010000327 DAZPJP010000000
VERSION     DAZPJP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.330"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            14..568
                     /locus_tag="ACHU9J_004339"
     CDS             14..568
                     /locus_tag="ACHU9J_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1907708.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(578..2005)
                     /locus_tag="ACHU9J_004340"
     CDS             complement(578..2005)
                     /locus_tag="ACHU9J_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1907709.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2002..2709)
                     /locus_tag="ACHU9J_004341"
     CDS             complement(2002..2709)
                     /locus_tag="ACHU9J_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1907710.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          775 a          655 c          609 g          678 t
ORIGIN      
        1 gaagcattca cacatgaata aaaaatcaat gtatatattc gcaattttta tagttgcagc
       61 aattacctgt attagccaac ccaagaaaac gacgttgcgt gataaagcca tggtgaatta
      121 tgcctttgat tatttaagat caccaggcag tctaccgtta accacggcag ccacggagct
      181 ttccgcgatt catggtcact caacatcgca atatcgcctt ggcgaatttt atcttcatgg
      241 tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc aatcagcaga
      301 acaggaaaat ccgcgcgcac aaagcaaact ggggtggatc tacctgaaag gcctgggagt
      361 caaacccgat acccgtaaag caattctctg gtataaggaa gcagctgaac aagggtatgc
      421 tcatgctcaa tatactttgg gtttgatcta cagaaatggc actggtatta atgttaacca
      481 ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa atgcggaaag
      541 attacttgcc ggacttcccg cacattaacg tcgactgtca cccgaagaga tggagtattt
      601 ttcctatcaa accggcaaag ataaaaatgt agaaaataat tttgatgacg cccagacagc
      661 ccagcccgcc atcgtcaacg ggcattggct gcggttgcgg ctccggctcg acggtttcct
      721 gaccggtaaa attcgccgcc tgaattaata atcgacgaat aattaatgct tccagtggac
      781 aacgatcttc tgcatcaagg cggctcacaa tcggcattaa tgattcttct gtcggttggc
      841 tggcctgcac taattgtttg gcaactaaat ataatggcca taagggatgt tctgtatcac
      901 gcgtgagtga gatccgcggc ttacgtcctt tgtgcaaaac catctcgacc agtgaacgcc
      961 ccagcgcaga gagtttttca tcgctgagta tttcgtataa atgaaaacgc gtacgggcgc
     1021 tgtcgccggg gacataaagt agctgtttac cctgatcgtt aaccagttca cgagcttcaa
     1081 tcatcatcac cactaacggg taataatcga cccgatgctg acggcaccat tgcaatatta
     1141 aagcatccgc ctgggtggag ggatactcgc gccagtagtc acaaagttct gccagcaagc
     1201 tttcgccttc tccacaatag acgcgttgcg cgtattcata ataacgcgca tcttcattat
     1261 caggttcagc ttcccgccac gaccgggcgt aatccagaag ttcggggata tccaggcgac
     1321 ctgcgctata ccagcggagt aaatttctgt gcagttttgc atcatcgggg atgatcactg
     1381 cgcgatgttg cgctaaatac aatgtcagcc attctggatg cgatgaccaa atccgagcga
     1441 acatttggta aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc agaatggtct
     1501 ggaagttaaa aacattcccc ttcgcaatgg catataaaaa ggattccagt tcttcttcgt
     1561 ctatttcccc gacgttttcc tcctgcaacc accgcaatct ttctgctaat aacaccacgc
     1621 actcgaagga taaatgggca gtgttcatgg cgattgtgca cagcgaccag cgtaattcat
     1681 caatctcttc catcgagcaa taatttaatt gctgaatgaa tcgctgccag gcggagggca
     1741 gaaaacgttc gctatcagag gcaagtaacg cgcgaaaggc gagcagaatt tcatgttctg
     1801 ctacctcata ttcttctggt tgccaaacag atttagcagg cgactgcgca atccggagcg
     1861 cctcctcata cgcctgacga agttgtttaa aaccctgcgg atcggtttct ggatgaaagg
     1921 acggtaacag cgccagataa gcgcggcgga taatatcgac gtcagtcgtt tcctctattt
     1981 cgaggatctt ccagcaattt ttcataggtc gctcatatcc ttaagcgttt ccgggcatgt
     2041 ggggtagtca gccagtggtt gccaggacaa ggcagggtaa tacgcatcat cactggcaaa
     2101 taattgataa aacatatcat tgccggaatc gtcgtattct ttacgagcta agaattcgcg
     2161 taataaaggc agatgatcat cttcgggaga ctgccaatat tgccgaccaa aggtataacc
     2221 ggcaaagtat tgccgccagt tactataata acgtagagcc cgtagatgaa tacgcgactg
     2281 gatccataga ctttcttctt cactcaacca gttattaagt acgcccattc gacttaaaaa
     2341 acccatccgg acataatccc atgctttaat tcctccctca ccacagcatt cggctgtgct
     2401 ggcgacaaat tgcgcataag cctgaccttg ctcatttagt tcagcaagat agtcacgcca
     2461 ttcacatggc gaataacgaa accagcggtg ataaaaccct gccaaccgag cggcatgacc
     2521 gttatcgatc attcggtgaa ttgtgcgcag caggtcatca cggtcatcaa ttccccatga
     2581 ggaagaaagg tcaaaaaagc atgtacctaa atacattttg ggggatgaat gcgaaggtaa
     2641 acagcattca ttgcgtattt ccatcggagt ggaaagtgca aacaatattt tttgcgaccc
     2701 catgtccata taattta
//
LOCUS       DAZPJP010000328         2703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPJP010000328 DAZPJP010000000
VERSION     DAZPJP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.331"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHU9J_004342"
     CDS             <1..247
                     /locus_tag="ACHU9J_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR1907711.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(287..1495)
                     /locus_tag="ACHU9J_004343"
     CDS             complement(287..1495)
                     /locus_tag="ACHU9J_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1907712.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHU9J_004344"
                     /pseudo
     CDS             1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHU9J_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2022..2690)
                     /locus_tag="ACHU9J_004345"
                     /pseudo
     CDS             complement(2022..2690)
                     /locus_tag="ACHU9J_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          747 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgcg acgcggcggt
      121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg
      181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgctg acagaaaccg
      241 atattgacat cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct
      301 gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg
      361 caagaacggc gtgccccgcc gctaaaatgt tacgagcacc atttacatcg gcgttcgctg
      421 tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttctct ttcgctgtat
      481 gaccacagta cgcgcaacgc tggcttgtat acgctggcgg aacagcaagc acctgaccgc
      541 cacgccagag ctgcttgtac tcaagctggc ggtgcatttc ataccagccc tgatccagta
      601 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
      661 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
      721 tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat
      781 gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc
      841 ttaactgtcg ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct
      901 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
      961 ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc
     1021 acgtaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat
     1081 tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct
     1141 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
     1201 cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
     1261 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
     1321 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
     1381 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
     1441 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc
     1501 aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt
     1561 tcctgatgca tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg
     1621 acagaaaaac tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt
     1681 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg
     1741 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
     1801 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
     1861 agtagctgcg gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
     1921 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
     1981 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
     2041 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
     2101 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
     2161 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
     2221 tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
     2281 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     2341 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     2401 gtatagttca aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc
     2461 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     2521 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     2581 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     2641 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     2701 ctg
//
LOCUS       DAZPJP010000329         2651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPJP010000329 DAZPJP010000000
VERSION     DAZPJP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.332"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHU9J_004346"
     CDS             complement(25..315)
                     /locus_tag="ACHU9J_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1907713.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHU9J_004347"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHU9J_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1907714.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHU9J_004348"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHU9J_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1907715.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHU9J_004349"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHU9J_004349"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2651)
                     /locus_tag="ACHU9J_004350"
     CDS             complement(2171..>2651)
                     /locus_tag="ACHU9J_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1907716.1"
                     /translation="AVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAR
                     EARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGD
                     ADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          698 a          718 c          600 g          635 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
     2521 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
     2581 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
     2641 aggggactgc c
//
LOCUS       DAZPJP010000330         2589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPJP010000330 DAZPJP010000000
VERSION     DAZPJP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.333"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHU9J_004351"
     CDS             <1..187
                     /locus_tag="ACHU9J_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907717.1"
                     /translation="GAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQI
                     ERNAKARIRRATKSRTE"
     gene            280..498
                     /locus_tag="ACHU9J_004352"
     CDS             280..498
                     /locus_tag="ACHU9J_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1907718.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            500..865
                     /locus_tag="ACHU9J_004353"
     CDS             500..865
                     /locus_tag="ACHU9J_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1907719.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            862..1527
                     /locus_tag="ACHU9J_004354"
     CDS             862..1527
                     /locus_tag="ACHU9J_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907720.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1527..1892
                     /locus_tag="ACHU9J_004355"
     CDS             1527..1892
                     /locus_tag="ACHU9J_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1907721.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLNDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2347..2502
                     /locus_tag="ACHU9J_004356"
     CDS             2347..2502
                     /locus_tag="ACHU9J_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1907722.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          715 a          544 c          699 g          631 t
ORIGIN      
        1 cggtgcggtt gtggagccgt ttgactggat gctctctgtg gcaaaaagag aaaccagact
       61 ggcagatgat gtaaggctat tgcgccagga ggaacaggaa aggcggagaa atatagaaaa
      121 gctgatacag attgagcgta acgcgaaagc gcggatacgc agggcgacaa aatccagaac
      181 tgaataatta aatttagcac tgttaaaaat ttaatcctta accggaggga tttctgcacc
      241 ctcaaatcat caggagaccg ctcgaaaggg cggtaatgaa tggttacatt atttagaaaa
      301 aaatatccgc gaaagagtag aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg
      361 atgacaccga tatccccgct aatttttgtc tggataatcg gaaaaataat tgagccagtt
      421 attgaattgt ataacgacgt ggtatgggcg tcattcaaca cactgcacaa taaaattaat
      481 ccgtataagg aaagctgata tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa
      541 gttcggtggt cgctgcgctt attgcggctg cgaacttggc gaaaagtggc atgcagacca
      601 tgtaaaaccg gtcattcgtt ttgatggaaa tatgcttcac caggaacgtg acgatatatc
      661 caacatggta ccagcatgcc acccatgcaa tctgcacaag cattgcagta gcctggaaga
      721 ttatcgacga attatcagtg atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc
      781 gctggttcgt atgggattgg ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa
      841 atatcaagaa ggggctacgg catgaccact attaccaaag agcgactgca atggctggct
      901 aacatttctg gccgcgatga tattgacgat atagacggcg gtgaaattcg tgaactggcg
      961 cttatcgctc tggcatcact ggaagcagag cctgtagcgg aatgcattgt tgaagatggg
     1021 ggcatgtgta ttgacgggtt cggtgagtat gtgggtcact cgctgcctga tggaatgcat
     1081 gagctttatg ctgccccgcg aatgcgacaa cctgatggat ggaaagccgt agctgtagcg
     1141 tggaaggtga cgtttactca agttgaccag gaatctaata cgttcactgc tatatatttt
     1201 gacaaagcgg aagttgagtg ttgggtacga ctgcataaag catgtgattt tcgggcagat
     1261 ataacaccgc tttacgcagc gccggcagtg ccggttgcag taaacgacga catggcttac
     1321 gcattccatc acgcactgtc agattcatcg cttggctctg atgaaatcga agaaattaaa
     1381 accggtttgc gtgctgcctt tgccaacgtc actatccaac cagagccgat agtgccggat
     1441 gaaatcgggc caaacgatag taatacgttt gattatgttg atggctggaa cgcctgccgc
     1501 gctgccatgc ttaagggaga taaataatga ttaatcgaac caaactggag cacattctcg
     1561 aatatgccag gcagcagaaa tgtattgggc aactttgtaa aattccacca ggagatatgg
     1621 ttgaaatcgt ggaaatggcc atgcgtaagg ctggcaactc tccggtagct ccggctggct
     1681 ggataagctg tagtgatgca gttcctgctg aatattgcga tgtgattctt ctcaatgatc
     1741 tcgggaatgt attccccggt tcctgggata aggttttttg ccccactcgt ggcgggaata
     1801 agatggcttt tgtggacaaa gacggcgttg aagtagagag ctcaactcac tggatgccgc
     1861 taccggaacc accgcaggag gtgaatcaat gacctggcct gaggcattca ccacggtagg
     1921 aattgcgatg gcggtggcgc tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa
     1981 aaccccggat tgatggtccg gggtttttga aggaaacaaa acagaaacag caattgccgt
     2041 tacctgttgt taccatggca agtaaacgta tctcaggcga gcgcattgcg ccgttctgac
     2101 gcagataaac tagcctggat agatggtgct ggcaataaaa aatagcgttt tcttatcggt
     2161 gtcggtaaga ttgctgcggg tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca
     2221 gacagagaaa agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag
     2281 gcccaatcta tgtctcacaa atgtaggtta gcctcttacg tgccgaaagg caaggagaag
     2341 caggctatga agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc
     2401 attgtgacgg cactggtaac gaggaaagac ctctgcgagg tacgaatccg aatcggccag
     2461 acggaggtcg ctgttttcgt agactacgaa tctagagagt aagagtgacc aggcgggaga
     2521 gtaatctccc gccacctttg atgtgtcagg catcctcaac gcacccgcac ttaacccgtt
     2581 tcggcggtt
//
LOCUS       DAZPJP010000331         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPJP010000331 DAZPJP010000000
VERSION     DAZPJP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.334"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHU9J_004357"
     CDS             196..1149
                     /locus_tag="ACHU9J_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1907723.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHU9J_004358"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHU9J_004358"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1907724.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHU9J_004359"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHU9J_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          636 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZPJP010000332         2562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPJP010000332 DAZPJP010000000
VERSION     DAZPJP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.335"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHU9J_004360"
     CDS             <1..81
                     /locus_tag="ACHU9J_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907725.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHU9J_004361"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHU9J_004361"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1907726.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHU9J_004362"
     CDS             1027..1485
                     /locus_tag="ACHU9J_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1907727.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHU9J_004363"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHU9J_004363"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1907728.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHU9J_004364"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHU9J_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1907729.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
BASE COUNT          643 a          630 c          678 g          611 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg ga
//
LOCUS       DAZPJP010000333         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPJP010000333 DAZPJP010000000
VERSION     DAZPJP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.336"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHU9J_004365"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHU9J_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1907730.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHU9J_004366"
     CDS             complement(390..1178)
                     /locus_tag="ACHU9J_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1907731.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHU9J_004367"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHU9J_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1907732.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHU9J_004368"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHU9J_004368"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1907733.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHU9J_004369"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHU9J_004369"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1907734.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZPJP010000334         2523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPJP010000334 DAZPJP010000000
VERSION     DAZPJP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.337"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHU9J_004370"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHU9J_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHU9J_004371"
     CDS             829..1674
                     /locus_tag="ACHU9J_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1907735.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHU9J_004372"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHU9J_004372"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1907736.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHU9J_004373"
     CDS             1861..2061
                     /locus_tag="ACHU9J_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1907737.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHU9J_004374"
     CDS             2146..2421
                     /locus_tag="ACHU9J_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907738.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          735 a          549 c          596 g          643 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atg
//
LOCUS       DAZPJP010000335         2515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPJP010000335 DAZPJP010000000
VERSION     DAZPJP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.338"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(209..466)
                     /locus_tag="ACHU9J_004375"
     CDS             complement(209..466)
                     /locus_tag="ACHU9J_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907739.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(463..960)
                     /locus_tag="ACHU9J_004376"
     CDS             complement(463..960)
                     /locus_tag="ACHU9J_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1907740.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1162..1620)
                     /locus_tag="ACHU9J_004377"
     CDS             complement(1162..1620)
                     /locus_tag="ACHU9J_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1907741.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1617..2114)
                     /locus_tag="ACHU9J_004378"
     CDS             complement(1617..2114)
                     /locus_tag="ACHU9J_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1907742.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2114..2329)
                     /gene="essD"
                     /locus_tag="ACHU9J_004379"
     CDS             complement(2114..2329)
                     /gene="essD"
                     /locus_tag="ACHU9J_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1907743.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          593 a          661 c          579 g          682 t
ORIGIN      
        1 tttggctgaa attgggatgt ctttccatca gtccgccacc acaaagaatc ttttttgcca
       61 taaggcagga ggttcatctt tcagtggctg ccggtgttat ttccccactt actggcttgg
      121 gttgtttcgt ggtactgccg ttaactggtg gcccagaata aattccggtt tcattatcaa
      181 gcccacccgt aaatgggctt tgtaatggct acttcgcttt tgcttccgct cgcttacgcc
      241 ggcgctcttc tttcctctcg gcttttgcca tgtccatgaa tgcctgcatg atcgagttcc
      301 gcatcatgta gctaacaaag tgatgattga cacagccgtt gaggcgcagc tgctcgccaa
      361 actcatccac cgaggccaat gcttccatca tgcccttctc gcctttcatg aactctgaga
      421 agtcgcgccc cgctctggag gcgcattcaa taacatgatc actcatcccg gaagcccggg
      481 gatcgtaatc tgcagctggt tagccaggga gttaatctca gcgaccaaca ctggcttcgt
      541 atagcgccat gctgccagcc cttgtccgca gaagctcgcc atgtctttct tctggtcaaa
      601 ttcatgacat ttcatgttga gctgcgcact taagctgttg cgatgctgaa gttctccggt
      661 gaagtagtca tcgaggactt tataggccgc gtacttgaac ccggggttta accaagccgc
      721 ataatcgtaa gcaacaaact tcccgccata tgttccaccg tgtacaccgc gctcagtaaa
      781 aaccacagat tcgtggtttt tctccagctc ggctaagaac tctttggtct gcttgtttcg
      841 caggtagtgg taaggcgatt cagattcact tttaccactg gctttccaca tatcagtgag
      901 gcagatcatg ccatcttcac cgatacgaat tggttgattg aagagggtta atgatttcat
      961 agcgtgtacc tactctttga aatgaacctt tgccgcacag gaaaccagcc caccgaggct
     1021 cgccagcact aactggtatc ctcaaaggcc cattccaaag gggcaggttc ggtgtaaaaa
     1081 acatgcgttg cggtacgcat ttattgcaaa aagccccgca tcgcgaggct cattaaatgg
     1141 actttgtgat ttgcaaaaaa attatttcag gcattgcgtc ctgatgtatt cctgcaggta
     1201 gttaacctgc gcggttatct tgtcgattcc acttcggaga cggtaataat tgagttcagc
     1261 atctgctgta agtcctgggc tttctccatc gcccatgccg ctggctccgg tcgttgactt
     1321 tgcacaggtg gcggcgactt gcaggcgctt acgcccagca gaaacatcag cacggagact
     1381 ttcgatagtc gcgttagcat cagcaagctc ctttatgtat cttgcgtcga gttctgctac
     1441 atcacgttga cgcttccgca tgtcagcgat ggtggcgttc gccttctcca gttcactggc
     1501 cttgttatcg cgctgctctt tgtaggcgat tgcgttatca cggtaatgat taacagccaa
     1561 tgacaggcag acgatgatgc agataaccag agcggagata atcgcggtta ctctgctcat
     1621 tgtttccccc acaaacagac ttcacgctca atctcacggc gagtcatcag tcctttccat
     1681 tgattaccgc cagcgtatgt ccagcgccgt agctgatcac atgcgccttt aatatcaccc
     1741 tggtttattt tgcgaagaag cgtcgatgtt ctgaaattgc cagcacccac gttgtagacg
     1801 aacgagtaaa gagcgcagcg cgttgtttcc ggtatatcga ctttgatgta cgggttaatt
     1861 tgtctggcga cagtggcaag gtctttattc aggagggctt tgcactctgc ttcggtatac
     1921 gttttaccgg gaatgatgtc ttttcctgta tgcccgtaac atacagtcca tacaccaact
     1981 atgtctttgt aaggattatg tctcacacct tccagaccat cgttaccacc tgggccagtg
     2041 attaacacag atgctatagc aatagccccg ccaccaatag cagcagcaac agcttttcgt
     2101 aatgatggag gcattatcca cctctcgcag ccttgcgctt atcttcttta atcttgaaat
     2161 aaaggtttgt caggtacgtc agcaggccaa ataccaggct acccagcact ccaattgccg
     2221 cccactgtga gggcgtgact ttatctagca gctgtaaaaa ccagtacccg gcactacctg
     2281 ctgaggtgcc ataggcgaca cccgttgtta acttatccat ggatttcata accccacctc
     2341 gcagacaaag cgggtgtaaa ttgagggaat actacgaaac gtaacagact cggagtcagt
     2401 gaataactca ggtattgggt tatcagctaa tatcgagact caaaaaatgg aaaaacccgc
     2461 tcgacggcgg gtttaagctg tgtgacgaag taaccactct taacagcata accaa
//
LOCUS       DAZPJP010000336         2478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPJP010000336 DAZPJP010000000
VERSION     DAZPJP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.339"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHU9J_004380"
     CDS             <1..275
                     /locus_tag="ACHU9J_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907744.1"
                     /translation="ITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLR
                     LQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(633..767)
                     /locus_tag="ACHU9J_004381"
                     /pseudo
     CDS             complement(633..767)
                     /locus_tag="ACHU9J_004381"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(780..1601)
                     /locus_tag="ACHU9J_004382"
     CDS             complement(780..1601)
                     /locus_tag="ACHU9J_004382"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1907745.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1590..2478
                     /locus_tag="ACHU9J_004383"
                     /pseudo
     CDS             1590..2478
                     /locus_tag="ACHU9J_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1870..1986
                     /locus_tag="ACHU9J_004383"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          645 a          565 c          579 g          689 t
ORIGIN      
        1 ccataacaga ggccaccctg tacgaaaacg ggctgtatac gcgccacatc agactcagtc
       61 gcccagtatc tgacagcaaa taccccttgc cacccggatc tcagctcatt aaaaatatga
      121 ccttacgact gcgcctgcag gatcagcagg aagaaacatc cgtgacatta tttcagggaa
      181 aaatgagtac cgacggaaat aactattaca tcatcaaagg taaagtaagc tcagtacttc
      241 tgttattaaa aatgctgcag attaatcacg cctgatctct gtcccggtat tttagcccga
      301 ggtatattct gaaatatcat tcatcatgac cggcgtttat acaaacgccg gtcattcgct
      361 aacagcttca ccgttccttc ccccatccag gttccgcatg agcgtgttgt aatgttcata
      421 agcgtattat caggattgtc tggaaaattc ccgatacttt ggactattac tcctgaacaa
      481 ttcgaaattc agcagattaa aggtaagcgt taacggaggt aagcgttaac ggaggtaagc
      541 gtaaaccgac tgccgtatgt agccattaag ccagtattgg taacgtaggt gcccaccttt
      601 tcaaatgatc tgcgccccaa atcttggaca tcctatgaaa aatgtttaag tcgatattga
      661 accggactca tgccatcgag tttctcttta atgcgatggt gattgtagta ttcaatgtaa
      721 tcaattatat cttgctttag atgatgtaac ttattgtatt tatttaggta gtaacattct
      781 tattttaaag tactaaaaaa gctttccatt gctgcattat ctaagcagtt accacgtcgg
      841 gacatgcttt gtgttatgcc attatcactc aaccaacgtt gccaacgagg catctgatat
      901 tgccacccct gatcactatg caacaagggc ttatcgtctt tattgagttg ttctaccgca
      961 tctaatagca tgctctttac cattgatgct aatgggcgct cagtaaggtt atatgagact
     1021 atttctccat tatacaggtc catgattggt gataaataga gcttcctgcc atcgacctta
     1081 aactctgtga catctgttac ccacttttga tttgggcggt cagcataaaa ttgtctcttc
     1141 agtaaattat tacaagttcg cccctgttct ccacgatagg cacggtactt cttgcgcctg
     1201 ataagtgagc tgatacccag ttccttcatt aactttctga tggttttacc attaagtaaa
     1261 atgccctcgt tacggcaggc aagtctgatg cgtcgataac cataacgccc tttatgctga
     1321 tggtatattc tcaatattac ttgtttaaca agttgatatg gatctggcgc attaaggcgt
     1381 ttcacgtgat agtaaaatgt actacgtggc aaatccgcga taaccagtaa ttgtgataat
     1441 ggatgacatt gccttaactc attaataatg attacttttt cttttgttcc aatcgtaatt
     1501 tttcctgagc taaggctttc agctttttta gataatgaac cgccccggga atcctggaga
     1561 ctaaactccc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt cccccgaagt
     1621 ccgtcaacgg gcagtccgta tggttctgga aagtcagggc gaatatgact cacaatgggc
     1681 ggtaatttgt tccattactc caaagattgg ctgtacaccg gagactctgc gtgtctgggt
     1741 tcgccagcat gagcgggata ccaggggcgg tgatggaggg ctcaccaccg ctgaacgtca
     1801 gcgtctgaaa gagctggagc gtgagaatcg tgaactgcgc tgcagtaacg atatccttcg
     1861 ccaggcttcc gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aataatgcca
     1921 ctgctggata agctgcgtgg gcagtacggg gtcggaccgg tatgcagtga actgcatatt
     1981 gccccgtcaa cgtattacca ctgttagcaa caggacatca tcctgataaa cgcagtgccc
     2041 gtgcgcagca cgatgactgg ctgaagagag agatacagcg cgtatacgat gaaaatcatc
     2101 aggtgtacgg tgtgcgtaaa gtctggcgcc agttgttacg ggaaggaatc agggtggcca
     2161 gatgtacagt ggcgcgcctc atggcggtta tgggacttgt cggtgttctc cggggtaaaa
     2221 aggtccgcac taccgtcagc cggaaaaccg ttgccgcagg tgaccgcgta aaccgtcagt
     2281 tcgtggcaga acgtcctgac cagctgtggg tggctgattt tacttacgtc agcacatggc
     2341 agggcttcgt ctatgtggcg ttcatcattg atgtgtttgc cggatacatc gtgtggtgac
     2401 gagtctcatc gtctatggaa acgacattcg tgctggatgc actggagcag gcgttgtggg
     2461 cccgtcgtcc gtctggca
//
LOCUS       DAZPJP010000337         2448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPJP010000337 DAZPJP010000000
VERSION     DAZPJP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.340"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            41..1141
                     /gene="ompC"
                     /locus_tag="ACHU9J_004384"
     CDS             41..1141
                     /gene="ompC"
                     /locus_tag="ACHU9J_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1907746.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1305..1787
                     /locus_tag="ACHU9J_004385"
                     /pseudo
     CDS             1305..1787
                     /locus_tag="ACHU9J_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1902..>2448
                     /locus_tag="ACHU9J_004386"
     CDS             1902..>2448
                     /locus_tag="ACHU9J_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1907747.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          801 a          491 c          454 g          702 t
ORIGIN      
        1 tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa aaatagttgc
       61 ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag tctataacaa
      121 ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt ttactgatga
      181 taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag aaacgcaaat
      241 caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct ataacgatga
      301 agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa aaattagtga
      361 atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg gttcatggac
      421 cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct tcatgacata
      481 ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa ttgaagggct
      541 gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc atctttatgg
      601 tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt atgtttatga
      661 tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc aggaaagagc
      721 cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca caggtataaa
      781 gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa acatgaccac
      841 ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg cgcaatatca
      901 attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca gagatctggg
      961 ccgctacggc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt atttcttcaa
     1021 caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa gcgactttac
     1081 ccgtgccgta gatattcgga ccgataacat cgtcgctacg ggcattacct atcagttcta
     1141 acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc tgctgtatga
     1201 ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca attaatatta
     1261 ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt atgaccaata
     1321 ttaatacagc ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca
     1381 aagaaaccat ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta
     1441 ataatggtga agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca
     1501 tggcatctct tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag
     1561 ttaatacgtt aaatctggaa aacaattgtc taacttgtct tgacttagct gacaatgcca
     1621 gtctcgttaa tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaat
     1681 caaaactgga aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc
     1741 agcattcatt ggttaatctg gaagcgcaaa ataacaaact gacaaaaatt aatatttctg
     1801 atagttataa actgaaattt cttaatcttg attataacaa gctagcatcg ctggatgtct
     1861 cccggcaaga atctctgatt gagttaagtg cccatcacaa tatgatcact gaccttatat
     1921 tacacaatca tcccaggatg aaaacaatca ctttaaacga caaccatatt gcacatttaa
     1981 acgccaaaaa cactacaaaa ctggaatatt taaacttaag caataacaat ttactgccaa
     2041 ccaatgacat tgatcaacta atatcatcaa aacatctttg gcatgtatta gttaacggca
     2101 tcaacaatga tccacttgca caaatgcagt actggactgc agtaagaaat ataattgatg
     2161 acactaatga agtgaccatt gatttatcag gacttaattt aaccactcaa ccaccagggc
     2221 tgcaaaactt cacatctatc aatcttgata ataaccaact cacacatttt gatgcaacca
     2281 actacgatag actcgtaaag ctaagtctga atagtaatgc tcttgagtca ataaattttc
     2341 ctcaaggcag aaatgtaagt attacacata tatctatgaa taataatgct ctcagaaata
     2401 ttgatataga taggctttca tcagttactt attttagtgc ggcacata
//
LOCUS       DAZPJP010000338         2416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPJP010000338 DAZPJP010000000
VERSION     DAZPJP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.341"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHU9J_004387"
     CDS             complement(<1..123)
                     /locus_tag="ACHU9J_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR1907748.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHU9J_004388"
     CDS             384..719
                     /locus_tag="ACHU9J_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1907749.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHU9J_004389"
     CDS             complement(1000..1131)
                     /locus_tag="ACHU9J_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1907750.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHU9J_004390"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHU9J_004390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHU9J_004391"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHU9J_004391"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2416)
                     /locus_tag="ACHU9J_004392"
     CDS             complement(1986..>2416)
                     /locus_tag="ACHU9J_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1907751.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRASWEK
                     TVKKFYDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          750 a          626 c          428 g          612 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc atagaatttc
     2101 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2161 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2221 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2281 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2341 accacctccc tgactttttt ccattcttcc cagtaagacg gatacacacc tttcgtacac
     2401 tttgcccata ccggcg
//
LOCUS       DAZPJP010000339         2389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPJP010000339 DAZPJP010000000
VERSION     DAZPJP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.342"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHU9J_004393"
     CDS             <1..164
                     /locus_tag="ACHU9J_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1907752.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..1267
                     /locus_tag="ACHU9J_004394"
     CDS             170..1267
                     /locus_tag="ACHU9J_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1907753.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDSQTQNETQQAGYDENIPV"
     gene            1318..1506
                     /locus_tag="ACHU9J_004395"
     CDS             1318..1506
                     /locus_tag="ACHU9J_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1907754.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            1611..1835
                     /locus_tag="ACHU9J_004396"
     CDS             1611..1835
                     /locus_tag="ACHU9J_004396"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1907755.1"
                     /translation="MYLTLKEWNARQLRPRSPETVRRWVRECKIFPPPIKDGREYLFH
                     ESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            1726..>2389
                     /locus_tag="ACHU9J_004397"
     CDS             1726..>2389
                     /locus_tag="ACHU9J_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1907756.1"
                     /translation="MNIYFMSQQKKLHLKRLAGFCRESEMAGRRRNSNTRDLPPNLYN
                     RGGYYSYRDPRSGREFGIGRDKRIAVNEAIAANMELLSTQHISLLDRIKGNSVTLFCE
                     LILNFRNEIERRQLSTNTMKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSK
                     FAIANQYRALLSDIFKTAIASGLAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADV
                     QNE"
BASE COUNT          790 a          487 c          552 g          560 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgcac ttgatgaaat
     1021 tatcgagttg ctcaacaagc accatggggg caccagtaac gacgccaaac gcgcaaaagc
     1081 tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa agaatcaaag gtatggactg
     1141 gccgacaatc aaagctggtc gtaatgcact atggattgaa ctggaaggcg ttgaatatgc
     1201 attcccggat tcacaaacac aaaacgaaac gcaacaagca ggatacgatg agaatattcc
     1261 ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa tattaatgga ggtgttcatg
     1321 tataaaatta cagccactat tgaaaaggaa ggtggttctc ctgccagctg gacaagatat
     1381 tcaaaatcta aactaacgaa atcagaatgc gaaaaaatgc tctcagggaa aaaagaagca
     1441 ggcgtttcca gagagcagaa agtaaaactg ataaatttta attgcgagaa acttctgtcc
     1501 tcgtgagttg cattatatca aaattagaac ttcatagctg attattaaaa atcaaccacg
     1561 cccgccagta ttttgtatat ttactggcgg tcatattaca ggaatatgac atgtatttga
     1621 cactcaaaga atggaacgcc cgtcagttac gtccacgaag cccagaaacg gttcgccgct
     1681 gggtgcgaga atgtaagatt tttccaccac cgataaagga tgggcgtgaa tatttatttc
     1741 atgagtcagc aaaaaaaatt acacctcaaa cgactggcgg gcttttgcag agaatcagaa
     1801 atggccggac gaagaagaaa ctcaaacaca cgtgatctac cacccaatct ttacaatcga
     1861 ggtggatatt atagttatcg tgacccacgt tcaggaaggg agtttggaat cggaagagac
     1921 aaaagaattg cagttaatga ggctattgcg gcaaatatgg aattattatc cacgcaacat
     1981 ataagcttac tggatagaat aaaaggtaat agcgtaacat tattctgcga gctgatatta
     2041 aatttcagaa atgaaataga gcgtagacaa ttaagcacaa acaccatgaa aagacacaac
     2101 cagaggttga aaatcataag tgaatacttt ggtggtgttc cggttaaaaa tataggcata
     2161 agggaggtgt acagctttct tgaaatcaga gcagctggga gtaaatttgc tattgctaac
     2221 cagtacaggg cgcttttatc agacattttt aaaacagcta tagcttctgg tcttgccgaa
     2281 gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag tcgactgtta
     2341 attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaat
//
LOCUS       DAZPJP010000340         2359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPJP010000340 DAZPJP010000000
VERSION     DAZPJP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.343"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..806)
                     /locus_tag="ACHU9J_004398"
                     /pseudo
     CDS             complement(1..806)
                     /locus_tag="ACHU9J_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(859..1455)
                     /locus_tag="ACHU9J_004399"
     CDS             complement(859..1455)
                     /locus_tag="ACHU9J_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1907757.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1482..2084)
                     /gene="yadL"
                     /locus_tag="ACHU9J_004400"
     CDS             complement(1482..2084)
                     /gene="yadL"
                     /locus_tag="ACHU9J_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR1907758.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2099..>2359)
                     /locus_tag="ACHU9J_004401"
     CDS             complement(2099..>2359)
                     /locus_tag="ACHU9J_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1907759.1"
                     /translation="SSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIR
                     WSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          664 a          507 c          471 g          717 t
ORIGIN      
        1 actaagcata gcggtaaaac aagtgggatt agtcaacttg acattgagca gatcgaaatc
       61 gattttgata aaataactat gctcatttat accttggcct gcgccatagg ctttaaatga
      121 atacagataa tttgatgagc tggataattt gatctgttga gttactgttg gatcaaagtt
      181 tgtatctgta tagaattcaa ttgtaagggt ctgataaata ccacctacag cccagtattt
      241 acgggtgcta ttcgcattat tgcaactcca atataacacg tcttcgcttg gattatacca
      301 cgaaaaatat tgttctgcag agtcaccaat gtatatccca ggtgaactaa cgttagttac
      361 ggtaccgtaa gctgaccaca tgtttgaaat taataatgtg taatacaaac caggaactga
      421 tgtattaaat aatttatggg agccatacat tttacctgag tcaaccattg cattttcaat
      481 acgcattgcg ctaccacttg tgctatcact tctgttgcaa taaagaaaac caccaccaga
      541 agcggtatct aactgactcc actgtaattt tacacctgta ggtccaagct gcgtcggttt
      601 gtagaaaact aaattatttg tacttgttgc atatctgaat atgacagtag catcgccatt
      661 cggccccgtt tctctccatt tttgatctga cggaccaacg ccgtaactat tcccaacaat
      721 atatcctacg ttatcatcag tgccagcact cgcggtataa caactcaagc acagaattaa
      781 aaaaaatgtc tgaagaattt cttcattatc actccaccat ctactgctgt aacagtcgca
      841 gttatttgtt ttgatctatt attcgtagta cacatcaact aatatatgac tcattacttg
      901 gccagatatg acatccagcg cgggatcgat tttttgcatt ctggcataaa atttccatga
      961 agaattatgc agggcagaag tcattactgg atacacggaa cgagaatttc ctgctgaact
     1021 aataacattg aatatattgg tcgggtcctg aatggaaaag agaacgacac cgacattttt
     1081 agccccggtt gcgacttcat tggcaaatat ttgccttgaa cctgcagcca tgacaccgct
     1141 aagggctcta aaatcaatat gtaactgatt aagcacatcg ccagttccct gtaatgcaca
     1201 gtctgatact gtgacagtga actcttttcc acctggttga tagctttcag gagtaacatt
     1261 atctgaaaaa tatcccaccc caacgacgcc aagatcaata ttaccctggt tactgattcc
     1321 gcttttgcaa gtgctgtttt ttacattggc aacaaggtca atatcctgcc cagcgatggc
     1381 tggataggta atgggaaact gtagtgacag aaacagaatt attctcttca tcagcttacg
     1441 ttgagtggga tgcatatata gttcctggat ctgtcagtct gttagttata ggttactgta
     1501 aatgtggcag gtgcagaaaa tttacccgca gtgacattgc ttacggtttt atttttttcg
     1561 acgaccagtc gagcactcat cggataataa acaaccgcgt tggctgcatt ggagatagtg
     1621 acctcttgag atgctggtgt cacacaacta aattgtgttg cgctccctgt tgccggagtt
     1681 tcggtgctcc agacttcaac ggcgacggca cttgctttat ctgcggctgt ggatccattt
     1741 gcgagccccg caccgtcatt agcagtacct tcgcatgtgg ctcgcttggt taattttatt
     1801 tgtgcttttt tattggggat acccgcacag tctttgaatt tgagtttgaa tgttttaact
     1861 ttggtcttgg cattgatttc agaaatatat acatcgccaa agtcaacaac agaaatgtcc
     1921 tgatcagagt catttactaa tgtagcggta caagtaccta tttctacggt agtaatcacg
     1981 gtcagcccaa tgctgtctgt tgccgcataa gagggtacgc tgaacaatga ggcagccaaa
     2041 acgacactgc tggataatcc ataacgtaaa tttttaaatg tcatcttaat tatccttctt
     2101 attgatagat gaaattaaac gttgccagtg cacgaaaatt ccccggtacg ccttgccctg
     2161 catctgtttc acgtagcgca acggtcattt caagaccctt atcgggctgc atctcgtctg
     2221 tgctccagcg tatcttttcc gcactgttag gtttaaggaa agtggcatca tcagtagtcc
     2281 gttttttgaa acccataccg atattacttg tcgtcgaaga tgaatcacca gactgcggta
     2341 taataagctt aggtgagct
//
LOCUS       DAZPJP010000341         2325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPJP010000341 DAZPJP010000000
VERSION     DAZPJP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.344"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(187..1191)
                     /gene="argF"
                     /locus_tag="ACHU9J_004402"
     CDS             complement(187..1191)
                     /gene="argF"
                     /locus_tag="ACHU9J_004402"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1907760.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1353..1769
                     /gene="rraB"
                     /locus_tag="ACHU9J_004403"
     CDS             1353..1769
                     /gene="rraB"
                     /locus_tag="ACHU9J_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1907761.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1815..2318)
                     /locus_tag="ACHU9J_004404"
     CDS             complement(1815..2318)
                     /locus_tag="ACHU9J_004404"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1907762.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          603 a          566 c          562 g          594 t
ORIGIN      
        1 cctccggaat ggcaaaatca atatatatgg atatattaat gaagaacagt taggaaaatt
       61 aaatcctccg gaagatttac gtatagcaat aaaaaaattg gggtggaata attgaactat
      121 cactgcataa aataatgtga cgccgggatg tttttgtatt tcccggcatc tttatagcga
      181 tatcaattat ttactgagcg tcgcgaccat caccgctttg atggtatgca tgcggttttc
      241 cgcctgatca aatacaatgc tggcggcaga ttcgaagacc tcatcagtca cttccattcc
      301 gccatgtagg ccaaactctt ccgccatttt tttgccaagc gtcgtttggt cgtcatgaaa
      361 cgcgggcagg cagtggagga atttgacctc cgggttaccg gtcaactgca tcatcttgct
      421 gttcacttga taatcacgca gcaatgcaat gcgttcagcc catttctctt ttgcttcacc
      481 catcgacacc catacatcgg tatagataaa gtcagcacct tcaactcctt tagctacatc
      541 ttccgtcagc gtaatattcc cgccattttg ttgtgccagg gcgcggcatt ccgtaaccag
      601 cgcagcttcc ggccagcatg cctgtggcgc gaccagacgc aaatccagac cggtaagcgc
      661 tgcagcttcg agcatcgaat tgcccatgtt gttacgtgcg tcacctgcat agactagcgt
      721 catttcgttg aacgttttgc cgggcaaatg ctcctgcatg gtgaggagat ccgccagcag
      781 ctgcgtgggg tgaaactcat tggtcaggcc gttccatacc ggcacgccag cgtattccgc
      841 cagtgtttcg acaatctcct gaccatagcc gcgatactga ataccgtcat acatgcgacc
      901 aagcacgcgg gcagtgtctt taatcgactc tttatgacca atttggctgc cgcttgggcc
      961 gagataagta acacgagccc cctggtcata tgcggcaact tcgaaagagc atcgggtacg
     1021 ggtcgagtct ttttcgaaga tgagcgcgat gtttttacca gtgagtttgg cttcttcttt
     1081 accgcttttc ttatcggctt tcagcttcgc ggctaactgc agcaggctgt tgagttcagc
     1141 tggcgtgaaa tcgagtaatt tcaggaaatg cttatgataa aacccggaca taggtccctc
     1201 ctgtggctaa cgccttaatg aattaaaatt caatttatat ggatgattat tcatttgcaa
     1261 gtctaaagca taaatcttta tcacaaaggt ggaggcaatg tcagtggtgt gtgacaataa
     1321 gagtatcggc aggacattaa gaggaatgag ccatggcaaa cccggaacaa ctggaagaac
     1381 agcgtgaaga aacacgtttg attattgaag aattactgga agatggcagc gatccggacg
     1441 cgttgtacac catcgaacac catctttccg cagacgatct ggaaactctg gaaaaagcgg
     1501 cagttgaagc gtttaaactc ggttacgaag tgaccgatcc agaagagctg gaagttgaag
     1561 atggtgatat cgtgatttgc tgcgacatcc tcagcgagtg cgcgttgaat gccgatctga
     1621 tcgatgccca ggttgaacaa ctgatgacgc tggcggagaa atttgacgtt gagtacgacg
     1681 gttggggcac ttactttgaa gatcctaacg gcgaagatgg cgacgatgaa gattttgtcg
     1741 atgaagacga tgacggggtt cgccactaat taattgatta aaatgcccga tagcacatac
     1801 tagcgggcat ttttttagag ttcgcgtagc atccgcactt cacaatcgac atggcccgtg
     1861 cagccaagcg catagtcgat atgctcaaag cccaaatgct cataaagcgc aatggcttcc
     1921 tttaaaaaag cggtcgtttc cagatagcag cgtttgaagc ccatctctcg tgcctgctcc
     1981 atcgccatta aggccagttt tttcgctagc cctttaccgc gaatagccgg aagaaaatac
     2041 atcttttgca gttcgcaaat gtccgactca ctcccggtta atggcgcaat cccaccgccg
     2101 ccgaccactt caccctcgta ctcaaccacc caatatgcat ggccaggctg actatacact
     2161 tgatacagct cgtcaagatt cggatcggcg acggtgtagc ctttatcagc ggtaagaccg
     2221 tattcggcgg atacctgacg aatgacgcgg gcgatagcag gattatccag cagcgtcagg
     2281 cgacgcatta caggtgattg cggcgcaatg ttattcatag tttaa
//
LOCUS       DAZPJP010000342         2304 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPJP010000342 DAZPJP010000000
VERSION     DAZPJP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.345"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..181
                     /locus_tag="ACHU9J_004405"
     CDS             <1..181
                     /locus_tag="ACHU9J_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907763.1"
                     /translation="KIKTTIITICALSYNLTAQASPSYRPRDRVNKTMIELQTNGVER
                     QLLDNSCGISSGVFK"
     gene            complement(199..534)
                     /locus_tag="ACHU9J_004406"
     CDS             complement(199..534)
                     /locus_tag="ACHU9J_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HFR1907764.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            663..1010
                     /locus_tag="ACHU9J_004407"
     CDS             663..1010
                     /locus_tag="ACHU9J_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1907765.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1030..1620)
                     /locus_tag="ACHU9J_004408"
     CDS             complement(1030..1620)
                     /locus_tag="ACHU9J_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907766.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1617..1877)
                     /locus_tag="ACHU9J_004409"
     CDS             complement(1617..1877)
                     /locus_tag="ACHU9J_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907767.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            1975..2279
                     /locus_tag="ACHU9J_004410"
                     /pseudo
     CDS             1975..2279
                     /locus_tag="ACHU9J_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          567 a          607 c          566 g          564 t
ORIGIN      
        1 aaagataaaa acaactatca ttacaatatg tgcattatca tataatctta cagcgcaagc
       61 ttccccgtca tacagacctc gtgatcgagt taataagact atgatagaac tacaaactaa
      121 cggagtagaa cggcaacttt tagataattc ttgtggcata agttctgggg tatttaaata
      181 aaatataaga caggctgtct atcttacaga cagccttttt ataataacag aaagaatata
      241 tatcatactt aacggaagag ataatatgaa aacagtaccc gtataaacag caagcaatcc
      301 catttttcca ggtgtatcag caaaaaaacc gctgttccag aaatcaggcc gggtaaattt
      361 cagagctgta tcttcaatat accattttgc aaccggatac aaaaccattc cgcagagaga
      421 aatacaccag aacagtaatc tgtatttaaa atcataatcc catgacatat acagcatata
      481 tcctcccgtc acccatcccc accacatatt attaaaataa tattttctgt tcatctgcca
      541 gttttccttt cttttgcact tttgtcagtg tactgatgca tgacaacata accacagcat
      601 gataataata atcaataaca ataagctgtg tcacgtttac atcatggaac ggaggtgctc
      661 ctgtgacttt tgagcagaaa aaagcccggg ccattgccct gatggacagc aaaaagatgt
      721 ggcggagcaa ttatgcccct cctcttctgc gtattctctg gcggctgggt atccggcttc
      781 cgccattacc ctttatgccg ttctggcagg tcacggtgct gacggggggc ttatggggca
      841 tttcctgggg atgtgcaatg tggtttatct actgggggcc gtcaggaatg gtggcaggtg
      901 aggccattat tataagtatc acggggggtt ttttgtccgg actgctcatg gcctctttcc
      961 actggtggcg caggaaagtt aaccgactgc caccatggga tgatgtgtaa accgtgcaca
     1021 ggcgtgttct tacggagcgg cactttcatt tatctgtacg cgaacctcca ttcctgcatc
     1081 gtgacaggcc tgcagccact gcgccacttc cagcggatcg ccctcccggc gtaccaccca
     1141 gccttcttta ttccataact gcagacaggt gctgccgtcg agacgcacca caaaatcccc
     1201 acggcaggcc tgatacgtta tgccgttcag ctccaccgcc agatcagtgg cagaagtgcc
     1261 gcctgcgggc agacctgctg ccgggaccag cggcagtacg acgacttctg ccgcacgctg
     1321 ttctgcccgg atgcggtcct gttctgccag cagcagaggc tgtgccacgg cccggccggc
     1381 gtccgtcagc tccactgcca gctgcaggtt cggagcgcgc agggtacgca gccagccggc
     1441 atcctccaga cgacggcagg aggcgcgcag gtttggcccg tagaccgggg cttccccgct
     1501 ttgctccagc acccgttcta tgtcccgcgt cagcacgggt cctggccgtt tttcatcaag
     1561 ggcagccagc acaatcagca ctctccgctg cagtggcgac ggtcggcggc tctgagtcat
     1621 ttccagtcat cctccggcca gatgagcttt ctgaggcgct gatggtcggc gtcaatgatc
     1681 tcctgtctct ttatggcatt cattgaggta tagccattta tggccagttc atgccagaag
     1741 atcagcacgg ctccggcttt ggctgactcg cagagataag ttacgccgtg gctgtccaga
     1801 ttgcattccc ggatgcgttt ttccgcaaac cgggtcagtt gttcatagct gagtgaaaac
     1861 gtcatttcct gatccatcct gcacgttcca tctgataatg ttctgacagg taaaaatcat
     1921 tgaaccgccc cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact
     1981 aaaaatactc gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt
     2041 cagggcgaat atgactcaca atgggcgaca atttgttcca ttgctccaaa gattggctgt
     2101 acgccggaga ctctgcgtgt ccgggttcgc cagcatgagc gggataccgg gggcggtgat
     2161 ggagggctca ccaccgctga acgtcagcgt ctgaaagagc tggagcgtga aaatcgtgaa
     2221 ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa ggcggagttt
     2281 gaccgcctct ggaaaaaatg atgc
//
LOCUS       DAZPJP010000343         2222 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPJP010000343 DAZPJP010000000
VERSION     DAZPJP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.346"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(282..1250)
                     /locus_tag="ACHU9J_004411"
     CDS             complement(282..1250)
                     /locus_tag="ACHU9J_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1907768.1"
                     /translation="MSEDKFLSEYSPKDSVWDSHRAFTDSVGGIYRSAAEFERYAERM
                     ASCSGLLRFGWSTLMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     AQFPSSRWLFLTLTVRNCEIGILGDTLTLMNAAFKRMEKRKELAPLQGWIRATEVTQG
                     KDGSAHPHFHCLLMVPPSWFSGVAYVKQARWVELWRDCLRVNYEPNIDIRTVKMKTGE
                     VMANVAEQLQSAVAETLKYSVKPEDMANDPDWFLELTRQLHKRRFISTGGALKNILQL
                     DRETNEDLVIADEMGDGTDDGKRTAFVWDSGRRRYRRAPEKDRFDN"
BASE COUNT          570 a          602 c          511 g          539 t
ORIGIN      
        1 aaaaaatagg ctaaattcat cgtcttaaag atgggggtcg cgcgtagcgc gcgacggtgt
       61 ggccgttttt gaccttcggg gttttggggc ggcgtccagc cgaagcaaaa ccccgtgtgg
      121 cgcgaagttc gggcgttcag ccggaacgaa gcaccacggg ttttcatgcc gttaatgggg
      181 tacagaaatg gggtgcactg atggggtaca tagcgtgctc tattgctgca ccccatcgtt
      241 gtaccccatc aacacaaaca aacatcgatg ggagcatatt ctcagttatc gaatctatct
      301 ttctccgggg cgcgcctgta acggcgcctc ccggaatccc aaacaaacgc agtacgtttg
      361 ccatcatctg tgccatcccc catttcatcg gcaatgacca gatcctcatt ggtttccctg
      421 tcaagctgca aaatgttctt aagtgcccca cctgtcgaaa tgaaacgacg cttatgaagc
      481 tgccgcgtca attcaagaaa ccaatcagga tcgttagcca tatcttccgg tttaacagaa
      541 tatttcaggg tttcagcaac ggcactctgt agctgttccg caacgttggc cattacttcg
      601 cctgttttca tcttcacagt cctaatatca atgttcggtt cgtaattcac ccgtagacaa
      661 tctcgccata gctccaccca acgagcttgt ttgacataag caacaccaga gaaccatgat
      721 ggaggcacca tcagcaaaca gtgaaagtgc ggatgtgcac taccatcttt tccctgagtt
      781 acttcagtcg ctctaatcca tccttggaga ggagcaagtt ctttacgctt ctccatacgt
      841 ttaaatgcgg cattcattag tgtcagagta tcccctaaaa tccctatttc acagttcctg
      901 acagttaacg tcaggaacaa ccagcgcgaa ctggggaatt gagcaacaat ttttggaaga
      961 gcctggtaaa aacgggcttg ccacatgagc gttcttctcc actggcagac aggacaatga
     1021 cggacacggc agaattgcgc actacgaagc cttaagcgcg tctctccagt ttccatgagc
     1081 gtagaccaac caaaacgcaa taaaccgcta caggaggcca tacgctctgc ataacgctca
     1141 aattcagcag cactccggta aataccaccg acagaatcag taaacgcacg atgtgagtcc
     1201 caaaccgaat ctttcgggct gtactccgac aggaatttat cttctgacat gccacctgct
     1261 ccgatctgtg caggcggtga tttgatgtgt tatattcatc acgtacctgt gcattgcgtg
     1321 ccagttactg tcaccgtctg acggctaatc ttccggtgac actttcaaat gctgaggagt
     1381 gcgctaacac tcctcagcgc cgcttaaaaa cacttcaatt ttgcgccaaa tcctttgatc
     1441 cgtcaaacac cactctaagc cgcgtcaata ctggcttgaa gcggtatttt caatccgcca
     1501 gttatccctt aagtataaat acaaggatcg cgcgcgcgag caacatccca aaaacgctca
     1561 ttctttaatc gtttttgggt aacctaatcg cttcaacatc gacggtctac gaccacctac
     1621 caaccgagta gctgctagtc ctgtgcaatt ttggcttagc agggggctaa cgcggtccct
     1681 gacgacatga aagcccttgg ttaggggtat cggcgcggcc tggcatttgt gattctctga
     1741 aaccgttttt cgcgccttac atgccgctga cgcggcatct tcccgccatc gggcatgact
     1801 taactaacag ccaacattag agagagcgat ccgctcgccg caaccctagc gaccgaacgt
     1861 tagtgagcga gtgggtgagg aagcggaaga taactgtaac ggacataatg cactcacgca
     1921 tcacagcata agaagaataa aagtattaca gcaaatatat tcttgctgaa ttatgccgat
     1981 atttcagcgc gggttttagc ttctgaaaca tccgcccaag tggattttca gaagctgggg
     2041 ggagccgccg aaaatttccg cctcagcgag aaataggcac gccaacgtgc cggtttcggt
     2101 ggggggcttg ccccgccaca ccgtcaagcc aggtaatatt tcccgcccca gcgggggata
     2161 ttaccgatta ggcgcgaccc ccatctttaa agatgaattc ccattttttc aagtttacgc
     2221 ag
//
LOCUS       DAZPJP010000344         2177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPJP010000344 DAZPJP010000000
VERSION     DAZPJP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.347"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHU9J_004412"
     CDS             complement(<1..458)
                     /locus_tag="ACHU9J_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1907769.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHU9J_004413"
     CDS             complement(497..784)
                     /locus_tag="ACHU9J_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907770.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHU9J_004414"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHU9J_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1907771.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHU9J_004415"
     CDS             complement(1417..2082)
                     /locus_tag="ACHU9J_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1907772.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          555 a          568 c          494 g          560 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcggg
//
LOCUS       DAZPJP010000345         2138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPJP010000345 DAZPJP010000000
VERSION     DAZPJP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.348"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..800)
                     /locus_tag="ACHU9J_004416"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="ACHU9J_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(802..984)
                     /locus_tag="ACHU9J_004417"
                     /pseudo
     CDS             complement(802..984)
                     /locus_tag="ACHU9J_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            complement(1006..1317)
                     /gene="traL"
                     /locus_tag="ACHU9J_004418"
     CDS             complement(1006..1317)
                     /gene="traL"
                     /locus_tag="ACHU9J_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1907773.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(1332..1682)
                     /gene="traA"
                     /locus_tag="ACHU9J_004419"
                     /pseudo
     CDS             complement(1332..1682)
                     /gene="traA"
                     /locus_tag="ACHU9J_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(1728..>2138)
                     /locus_tag="ACHU9J_004420"
     CDS             complement(1728..>2138)
                     /locus_tag="ACHU9J_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1907774.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          529 a          596 c          503 g          510 t
ORIGIN      
        1 gcaccggtat gacgacgggc ggtacggtaa cctttctcaa taaactcacc gaccttgtga
       61 tttttgaagt ccagcagacg ccagccttca tcaatgacgt tcagtttctt gaggttacgc
      121 ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg agaacatcac cgcaaccagc
      181 agtgacggac ggtcttccag tccgcccagc tccagcacca ccatttttgc gtcatcccgc
      241 agggacggct catcagagtt aaaatactgg ccgtaagtgc cgttggcagt gtactggtca
      301 agcagcacaa tcatttcgtc cagacggctg cggatagtcg gtgacccggc atactgctcg
      361 ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa tacgtgccct gttctctttg
      421 gccagccagg aggccctgac cgcctgcagc agcagacctt catgcacttc atccaggtta
      481 ccgttggggc tggccatcac tgacaactgg tcacggacac gctccgctga ctggtcgata
      541 tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac cgtccagata tacgcccccc
      601 atgttctcac acagggactt gtagccatcc cccatgtcga acaccacggc aaagcccccg
      661 gagtccagca cgctgcggat aagtggctgt atcagcccgg ttttaccggc cccggaggtg
      721 ccacagaccg ccatgttgta gttggtgtta ttcattcccc ggaagaaaat atcgataaac
      781 gccagctggt tacggtaggt gtgacacggc aaagggcaca ttaaatccca ttggggtcac
      841 ggcagtgcgt tgctcattct gaagacggta gttattcacc ccctgaatga ggttaacaga
      901 gaggctgaga acaataagca ctgacataaa tataaaggcg atggccatta cacgactggt
      961 acttaaacgg gcaccgtgtt ccatataaca atcctggtat cagttctatt taatccactg
     1021 ccggaaacac gaatcgggaa cattatgaaa aataccgcgc agcagggctg ttggcatata
     1081 ccagtaaatc aggtcacgta accaggaact gccccgccct tttttcagtt ttttaatccc
     1141 gaaataaacc agaaccgctg caccaatacc gaacagatat ttcgatgttg tgatacccca
     1201 gccaacacag attgctgcgg ggatcagttc atccagtggc aggccaaacc agcggctctg
     1261 gttggtcagt gtttccggga aacgatattt cttaagttta ttctcgtctc ccaacatcgt
     1321 tttatttcct gtcagaatcc ggcaacagac atacctactg caataaatac agagagtatg
     1381 gcaaagccga ccaggaactt gacgtttttg gtcatcatgt acatgactgc accgaccaga
     1441 acttcagcca gaacaaccca tttaacgata ctggagtcct taccgaaggt cgccttaacc
     1501 gtttcctgac ctttagccat caggtccgtg cctttaccag cagccatagc taactcaggg
     1561 aacaacatca tcagaacagc aaccgggatc actgcacgaa ccaggcgaag catattcaga
     1621 cgagtgaatt tagaaaaaaa cgatttcttt ttgacgggcg cagaagtacc ctgaacactt
     1681 aacgctgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga
     1741 tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg agtatggccc
     1801 agcctttcca gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggccagttcc
     1861 acttctgcac ggtttttcca gctcttacgg tgtattacct ccgctttgta aagaccattg
     1921 atgctctccg ccatcgcgtt gtcatacgag tcgcctgtac ttcctgttga tgccagtaat
     1981 ccggcttcct taagccgctg tgtgtaggcc agcgatacat actgagaacc tttatcactg
     2041 tgatggaccg tgccggacgg tcgacgggcc cataacgcct gctccagtgc atccagcacg
     2101 aatgtcgtct ccatggacga tgagacccgc caccccac
//
LOCUS       DAZPJP010000346         2131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPJP010000346 DAZPJP010000000
VERSION     DAZPJP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.349"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHU9J_004421"
     CDS             complement(<1..1094)
                     /locus_tag="ACHU9J_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1907775.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..>2131)
                     /gene="abgB"
                     /locus_tag="ACHU9J_004422"
     CDS             complement(1131..>2131)
                     /gene="abgB"
                     /locus_tag="ACHU9J_004422"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1907776.1"
                     /translation="SGKTFMVREGVFDDVDAALTWHPEAFAGMFNTRTLANIQASWRF
                     KGIAAHAANSPHLGRSALDAVTLMTTGTNFLNEHIIEKARVHYAITNSGGISPNVVQA
                     QAEVLYLIRAPEMTDVQHIYDRVAKIAEGAALMTETTVECRFDKACSSYLPNRTLENA
                     MYHALSHFGTPEWNSEELAFAKQIQATLTPNDRQNSLNNIAATGGENGKAFALRHRET
                     VLANEVAPYAATDNVLAASTDVGDVSWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSI
                     AHKGMLLAAKTMAATTVNLFIDSGLLQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
BASE COUNT          539 a          536 c          546 g          510 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtgg catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag c
//
LOCUS       DAZPJP010000347         2124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPJP010000347 DAZPJP010000000
VERSION     DAZPJP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.350"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..420
                     /locus_tag="ACHU9J_004423"
     CDS             <1..420
                     /locus_tag="ACHU9J_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR1907777.1"
                     /translation="LWTLRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNE
                     IKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMV
                     INEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(474..1424)
                     /gene="corA"
                     /locus_tag="ACHU9J_004424"
     CDS             complement(474..1424)
                     /gene="corA"
                     /locus_tag="ACHU9J_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1907778.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1576..1632)
                     /gene="ysgD"
                     /locus_tag="ACHU9J_004425"
     CDS             complement(1576..1632)
                     /gene="ysgD"
                     /locus_tag="ACHU9J_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1907779.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1794..>2124
                     /locus_tag="ACHU9J_004426"
     CDS             1794..>2124
                     /locus_tag="ACHU9J_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907780.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          590 a          507 c          501 g          526 t
ORIGIN      
        1 ttatggacat tgcgtcctgt acagattttg tatacatact ctgatgctgg caattcagtc
       61 tttctggtcg ttgatcatct gccatggact gacagcgata aaataaattg gtatctgaag
      121 catcagaatg aaattaaaaa tcaacatccc ctaccagaag gtagttggca tacctggtat
      181 gtcattgata tcggtaatgg cttcactgac tataaaaaat atatcgaagg gccgtatgaa
      241 gatctctact gtttcccgac aattaagagc aatgataatt gcatcgtgaa aaattactta
      301 atggttatta atgaataccc ttatcgtaac actcatattg gtattaacga ttttactgag
      361 tatcaattaa cacaagaaaa taaaatagag cgagttttca atccgcatga ctttaaataa
      421 gacaattttt agaaataagc cggataaaaa aatagcttta tccggcactc atattacagc
      481 cagttcttgc gcttaaagta cagatacggt gccaggcccg cgaggatcat aaagataatc
      541 gcgcctgggt agccgaaact ccacttcagt tctggcataa actcaaagtt catcccatag
      601 ctggaagcaa cgagcgtcgg cggcaggaat accacggata ccaccgagaa gattttgatg
      661 atgcggttct gctcgatgtt gataaaaccc atcgccgcct gcatcaggaa gttcaccttc
      721 tggaacaggg attcgttatg cggcagcagg gattcgatat ctcgcaggat ttcacgcgcc
      781 tgctccagtt gcccacccgg taaacgcgct ttacgcacca ggaagttgag cgcgcgctgg
      841 gtatccatca gacacaggcg aactttccag ccgatatctt ccagttccgc cagagtggag
      901 agcgcctcgt cgtactcatc gccctgatgc ccttccataa tcacccggct caactgctcc
      961 aggtcgctat agatattttc aatttcatct gccaactgtt cgattttggt ttcgaacaga
     1021 tccagcagca gctcgtaggc gttaccgtct accatcgact ggctacgggc acgcatacga
     1081 tacagacgaa aagcgggcag ttcacgctca cgcagagtaa acagacgacc atcacggatg
     1141 gtaaatgcca cagtggagtt accggcgtga tcttccgcat cttcaaagaa gaagaaggag
     1201 tgaatatgca ggccgtcgtc gtcttcaaag aaacgtgccg atgcttcgat gtcttccagt
     1261 tccgggcggg ttgccaggct ctggccaagt tcagattgta cgcgcagtcg ctcgtcgtcg
     1321 tccggttcga caagatcaat ccatactgca tttacaaggg gttgtgactc ttcgacttcc
     1381 agccgggtca gtcggttatt ttccagttga aatgcgctca gcatgaccgg gactcccaat
     1441 gcttaaaata tcggacagtt cggtgggcac acagaaacaa attgggtttc agaccattaa
     1501 acagcctgac tcagcgcgac gggaaaaatt gaggtcgctg acaaccgcta aggctatcag
     1561 caaaaaggga tagccttagg agttgatcct ggatgacagg atagtgagcc agtatctact
     1621 gggtgtgtcc aaggcgaatg tcctcttaga gtgatcgtgc gcgcatgtta cgccagcaaa
     1681 attttgccgt caacacgcaa cagaacacca cagaacaata gttgaacttc gcgctttttg
     1741 gtactcgtca aggtcgctaa caggataaaa aaatccaaca tccaggacct ataatggcgc
     1801 atcggctact cattggtaaa ggaatgatca ccttgaatct caaacggatt tttctcgccc
     1861 tcaccttact tcccttgttt gctgttgccg ctgatgattg cgcactctca gatccgatgc
     1921 tgaccgtaca ggcgtatacc gttaatcctc aaacagagcg ggtgaaaatg tactggcaaa
     1981 aagccaatgg cgaagcgtgg ggaactttac atgctctgct ggtggatatg aatagtcagg
     2041 gtcaggtgca gatggcgatg aacggcggca tctatgatga aagctatgcg ccgctcggtt
     2101 tgtacatcga aaacggtcag caga
//
LOCUS       DAZPJP010000348         2122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPJP010000348 DAZPJP010000000
VERSION     DAZPJP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.351"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            92..601
                     /locus_tag="ACHU9J_004427"
     CDS             92..601
                     /locus_tag="ACHU9J_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1907781.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            598..2016
                     /gene="phrB"
                     /locus_tag="ACHU9J_004428"
     CDS             598..2016
                     /gene="phrB"
                     /locus_tag="ACHU9J_004428"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1907782.1"
                     /translation="MTTHLVWFRQALRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          509 a          531 c          570 g          512 t
ORIGIN      
        1 accaacaaaa tacttccccg caaaaactct gccccataaa attcatttac catcgtctac
       61 acttactcct ttataccgtt caggagcagt aatgaatcta caacgatttg atgacagcac
      121 cctaatccgt atctttgccc ttcatgagtt acatcgactg aaagaacatg gcttaacgcg
      181 cggggcgctt ctcgattatc acagccgcta taaactcgtc tttctggcgc attctcagcc
      241 ggagtaccgc aaacttggcc cgttcgtggc tgatattcac cagtggcaaa atctggatga
      301 ctattacaac cagtaccgcc aacgcgtagt tgttttgctt tctcaccccg ccaacccgcg
      361 cgatcacacc aatgttttga tgcacgttca gggttatttt cgcccgcata ttgattccac
      421 agaacgccag cagctggctg cgcttatcga cagttatcgc cgtggcgagc aaccacttct
      481 tgcgccgctg atgcgtatca aacactatat ggcgctttat catgacgcct ggctttcagg
      541 gcagcgttat ttcgaacttt ggccgcgtgt gattaacttg cgccattcag gagttttatg
      601 actacccatc tggtctggtt tcgccaagct ttacgtctgc acgataatct cgcactggct
      661 gccgcctgcc gcaattcgtc tgcacgcgtg ctggcgttgt atatcgctac accacgccag
      721 tgggcgacgc ataacatgtc gccgcgtcag gctgaactta tcaatgctca actgaatggg
      781 ctacaaatag cgcttgcgga aaaaggtatt cctttattgt tccgtgaagt ggatgacttt
      841 gtcgccagtg tcgaaatagt taaacaggtg tgcgcggaaa acagcgttac ccacctgttt
      901 tataactatc agtatgaagt gaatgagcgg gcgcgggatg tggaagttga aagagcgctg
      961 cgtaacgtgg tgtgtgaagg atttgatgac agcgtgatcc tgccgcctgg cgcggtgatg
     1021 accggtaatc acgagatgta caaagtcttt acgcctttta agaatgcctg gctgaaacgg
     1081 ctgcgggaag ggatgccgga gtgcgtcgct gcgccaaaag ttcgtagtag cggatcgata
     1141 gagccctcgc catccattac gctgaattat cctcgtcagt ctttcgatac tgcgcatttt
     1201 ccggtggaag aaaaagcggc gattgcgcaa ttacgccagt tttgccagaa cggtgccgga
     1261 gaatatgagc aacaacgaga ttttccggca gtggaaggca ccagccgttt gtcggccagc
     1321 ctggcaacgg gcgggttatc gcctcgccag tgcttgcatc gcttgttggc tgaacagccg
     1381 caggcgctgg acggtggggt cggtagtgtc tggcttaatg agctgatctg gcgcgagttt
     1441 taccgtcacc tgataacgta tcacccctcg ttgtgtaaac atcgtccaat tattgcctgg
     1501 acggatcgtg tacagtggca gagcaatccc gcacatttac aggcctggca ggaaggcaaa
     1561 acgggatacc cgattgttga tgccgctatg cgtcagctta acagcactgg ctggatgcat
     1621 aacaggctac ggatgattac agccagtttt ctggtgaaag atttattgat cgactggcgc
     1681 gaaggcgagc gatatttcat gtcgcagctg attgatggtg atttggcagc caataacggt
     1741 ggctggcagt gggccgcttc aaccggaacc gatgcagcgc cgtattttcg tattttcaac
     1801 ccgacaaccc agggcgagaa atttgatctc gaaggtgagt ttatccgcca gtggctaccg
     1861 gaactacgca atgtaccggg gaaatcggtg catgagccgt ggaagtgggc gcagaaagca
     1921 ggtgtgaagc tggattatcc gcaaccgata gtcgagcaca aagaggcgag agtacaaacg
     1981 ttggcagcgt atgaggcggc gcggaagggg aaataacaga cgcgtcaggc aatcgagccc
     2041 agatgccgga tgcggcgtga acgccttatc cagcctacaa atttgtgcaa attcaataaa
     2101 ttgcaggaaa tacgtaggcc tg
//
LOCUS       DAZPJP010000349         2093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPJP010000349 DAZPJP010000000
VERSION     DAZPJP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.352"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            270..725
                     /locus_tag="ACHU9J_004429"
     CDS             270..725
                     /locus_tag="ACHU9J_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR1907783.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            725..895
                     /locus_tag="ACHU9J_004430"
     CDS             725..895
                     /locus_tag="ACHU9J_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR1907784.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            888..1178
                     /locus_tag="ACHU9J_004431"
     CDS             888..1178
                     /locus_tag="ACHU9J_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1907785.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1175..1537
                     /gene="rusA"
                     /locus_tag="ACHU9J_004432"
     CDS             1175..1537
                     /gene="rusA"
                     /locus_tag="ACHU9J_004432"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR1907786.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1534..1674
                     /locus_tag="ACHU9J_004433"
     CDS             1534..1674
                     /locus_tag="ACHU9J_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR1907787.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1760..>2093
                     /gene="quuD"
                     /locus_tag="ACHU9J_004434"
     CDS             1760..>2093
                     /gene="quuD"
                     /locus_tag="ACHU9J_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR1907788.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLM"
BASE COUNT          541 a          486 c          556 g          510 t
ORIGIN      
        1 gtcattggtt agatccatag ttctggctgt gattaggtga aagtatacaa ggtttttaaa
       61 gcgaagatca ctgttttggc ttcaagatag ttttgattta tcgtaatcaa tccgccataa
      121 tcctctcatc ggagcctgaa caactccggt gacttctgcg catttaaggg gacttaaatg
      181 cgaccacaat ctgaactcat caccttgtca cagatgcaga aatgcacctg cgattttctg
      241 cattctgcgg tttccgttag ggaggccgta tgactctgcc agtagacggc atcaaactcc
      301 atcgcggtaa cttcgcggcc atcggccagc agattcagcc attgctggat gccgggcaat
      361 gcttacgcct gcaggttaag ccgtggcgcg agaagcgcag cctgtcgcag aacgcgctca
      421 gccacatgtg gtacacggag atcagcgaat atctcatcgc ccgcggcaag accttcgcta
      481 cgcctgagtg ggtcaaagac gcgatgaagc acacctatct cggctacgaa agcaaagacc
      541 gtgtagatgt cgtgtccggc gaggtcacca ccgtccaatc cctccgccat acgtcagagc
      601 tggaaaccgg cgagatgtac atcttcctgt gcaaagtcga agcctgggcg atgaatatcg
      661 gctgccacct gacaattccg cagagctgtg aataccagca gctgcgcgat aagcaggagg
      721 cgtaatggct acaccgctta ttcgtgtcat gaacgggcac atctacagag tatcaaatcg
      781 tcgtaagcgt aagcctgagc tgaagccatc cgaaatacca acactgctcg gatataccgc
      841 tagcctggtt gataaaaaat ggttgcgact ggcagcaagg aggaatcatg gctgatttga
      901 gaaaagcagc gcgtggtcgg gaatgccagg taagaatccc tggcgtatgt aatggcaatc
      961 ctgaaacgtc agtactggca catatccggc tggctggatt gtgcggtacc ggtatcaaac
     1021 cgccagacct gattgccacc attgcatgtt ctgcctgtca cgacgaaatc gaccgccgca
     1081 catattttgt cgatgctgag tatgcaaaag aatgcgcgct ggaaggtatg gcgagaacgc
     1141 aggttatctg gctgaaagag ggggttatta aggcgtgaat acctacagca tcacattacc
     1201 ctggcctccg agcaataacc gctattaccg ccataatcgc gggcgcacac acatcagcgc
     1261 agaagggcag gcataccgcg ataacgtcac ccgaatcatt aaaaacgcaa tgctggatat
     1321 cggcctggct atgccagtga aaatccgtat tgagtgccac atgccggatc gccgtcgccg
     1381 tgacctggat aatctgcaaa aagccgcttt tgacgcactc accaaagcag gtttctggct
     1441 ggatgatgct caggtcgttg attaccgcgt tgtgaagatg cctgttacca aaggtgggag
     1501 gctggaactg accatcaccg aaatggggaa tgaatgatgt ttgagtttta tatggcagaa
     1561 cttcttcgcc accgctgggg gcatctgcgc ttatatcgtt tccccggttc tgttttgacc
     1621 gattaccgaa tactgaagaa ttacgccaaa accctgacag gagcaggagt atgaagtcag
     1681 agataacaat caactaatac tgttttgttg atttttgctt gtaattggcg ttctggtctg
     1741 atttttgtgg agtaagttga tgcgtgatat tcagatggtt cttgagcgtt ggggagcgtg
     1801 ggcggctaat aatcatgaag atgtgacctg gtcgtccatt gccgccggtt ttaagggatt
     1861 aattacttca aaagtaaaat ctcgcccgca atgttgtgac gatgacgcga tgattatttg
     1921 cgggtgcatg gcccgtctga aaaagaacaa cagcgatttg cacgatttat tagtagatta
     1981 ttatgtagtc ggtatgacat tcatgtcact ggcaggtaag cattgctgct ctgatggtta
     2041 tatcgggaaa aggttacaga aggctgaggg cataattgaa gggatgttaa tgg
//
LOCUS       DAZPJP010000350         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPJP010000350 DAZPJP010000000
VERSION     DAZPJP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.353"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHU9J_004435"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHU9J_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR1907789.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHU9J_004436"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHU9J_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR1907790.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZPJP010000351         2064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPJP010000351 DAZPJP010000000
VERSION     DAZPJP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.354"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHU9J_004437"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHU9J_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..>2064
                     /gene="fimH"
                     /locus_tag="ACHU9J_004438"
     CDS             1816..>2064
                     /gene="fimH"
                     /locus_tag="ACHU9J_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HFR1907791.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYV"
BASE COUNT          516 a          476 c          531 g          541 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcctggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctggctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccacccgg cacgctggtg
     2041 aattatacat accgtagtta tgtc
//
LOCUS       DAZPJP010000352         2063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPJP010000352 DAZPJP010000000
VERSION     DAZPJP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.355"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHU9J_004439"
     CDS             <1..326
                     /locus_tag="ACHU9J_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907792.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHU9J_004440"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHU9J_004440"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1907793.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          507 a          508 c          482 g          566 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttgacattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaacacaatt ataaaaaatc ccg
//
LOCUS       DAZPJP010000353         2047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPJP010000353 DAZPJP010000000
VERSION     DAZPJP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.356"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..633)
                     /locus_tag="ACHU9J_004441"
     CDS             complement(<1..633)
                     /locus_tag="ACHU9J_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFR1907794.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYAC"
     gene            791..1900
                     /locus_tag="ACHU9J_004442"
     CDS             791..1900
                     /locus_tag="ACHU9J_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1907795.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT          534 a          559 c          532 g          422 t
ORIGIN      
        1 gcacgcatag gcaggcgcgg ggaacgtttc gtttttgcca cttttcgggt tgtaggcgat
       61 gaagtccggc cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc
      121 ctcagccatc gtgttacagt catgacatga ggcatacaca aacgcgcgca gtttacccgc
      181 aatcacgcac agggattttg tcaccgcctc cgtgtccagc tccggcgcgg ccagaatacg
      241 cggacggtat ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata
      301 gctgccgtca gattcagaac caccgataac cagttgatcc tgcgtttttc cgtcttcttc
      361 tttgtgttca gccacgcgaa cgacgatcac ctttgtgctc acctggtctg cgatggcctt
      421 aagcgcacga taaagcgtcc ccgttgttcc gcattttccc agcacgtcat tgacgcgggg
      481 cagcagtgtg ggcttgttca gcgggaacag ctccgcgtcc gcatcatccg ccgttgccac
      541 gataccgata acgctggaat caacatcgtt aatcgctgtt accaggtcgg tattttccgt
      601 aacacgggca ccatgaaaac gagtttcact catagcttca accccttgta tccgttaaat
      661 gattcggcaa caatcatcac ccaccacgcg cgtaatctca cccctgcgcc attctcccgc
      721 cacggcgaca acaaaaagca gtaaccccct ccgcacgcac atgcgaccat gccacacagg
      781 gagggagcag atgaccgaca ccaccatgca attgctcagt cagggcacag accccgtgaa
      841 aatgccggat tttgatattc tcgcggaggg taaaacgctg tcaggcgtgg cagagcgcct
      901 gatgagcctg tcactgaccg acaaccgggg atttgaggcg gaccagctca ccatcacgct
      961 ggatgatgcg gatggtcagt tgcagctacc gccacggggc gcgcgcctga cggttctcat
     1021 tggctggaag ggagaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca
     1081 cgaaggaccg ccggacaggc tgactgtttc agccagaagc gcagattttc gggctgaatt
     1141 taacgttaaa cgtgaggtgt cctggcatga tgtgaccgtt gagcgtgtgg tatccgccat
     1201 cgctcatcgg tatggtctga aaccgcaaat cagcgaaatg ctgatggata tcgaaatcga
     1261 ccacgccgac cagaccgaag aaagcgacat gtccttcctt acgcgcatgg cggaaatgct
     1321 gggcgcaatc accacggtaa aaagcggtaa tctgttattc atcatgcccg gtggtggcgt
     1381 gaacgcacag ggccagccgt tgccctcgtt cgccatcaca cgcagcagcg gcgatcgcca
     1441 tcagttccgc attgctgacc gcgaagcgta tacgggggta cgcgcttact ggcttgatct
     1501 taattacggg aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgtcaa aaccaaaaaa
     1561 ggagaaaagc agcagccggg aaggcgacta tatggaaggt gcggaaggca acgtgtttgt
     1621 gttacgcaaa acttatcaga acgagcaggc agcaagacgt gcagcggcgg caaagtggca
     1681 gcagctacaa cgcggagccg catcattctc catcacactg gcgcatggac gtgcagaact
     1741 ctaccccgaa atgcatggca cggtaacagg atttaaaagc gagattgata atcaggactg
     1801 gattattgca aaagccgagc acaccattga taacagcggc tttaccacgc agcttgagct
     1861 tgaggcaaaa atcccggaat ggatagcaga aacagagtga gcaacttaaa tgtattaact
     1921 cagaacggat caacacactc acaacataga gtcaatatag ctgctcgctt tgttccagca
     1981 aggatgccta tttctaagga aaaacagagc agaaacatat ggtgttttcc tacctgacat
     2041 caggggt
//
LOCUS       DAZPJP010000354         2031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPJP010000354 DAZPJP010000000
VERSION     DAZPJP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.357"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(153..623)
                     /gene="argR"
                     /locus_tag="ACHU9J_004443"
     CDS             complement(153..623)
                     /gene="argR"
                     /locus_tag="ACHU9J_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1907796.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1058..1996
                     /gene="mdh"
                     /locus_tag="ACHU9J_004444"
     CDS             1058..1996
                     /gene="mdh"
                     /locus_tag="ACHU9J_004444"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1907797.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          526 a          440 c          521 g          544 t
ORIGIN      
        1 tacataatgt gcttttttta ccgtatgtat tcattgtgtg aatgatatgt cgcggtaaaa
       61 cgcactattc gttaaattaa tcgcagggga aagcaggggg ttgagaggaa taagcaacat
      121 tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa cagctctaaa
      181 atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca
      241 tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag caggcgagca
      301 attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt gtagtcgata
      361 tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc agctggcagg
      421 cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt ggtcaacatc
      481 cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc ggcgacgatt
      541 tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt aactagttct
      601 tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca acattattgt
      661 gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa taatgttatg
      721 aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca cggcatgcaa
      781 attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct gaacggtagg
      841 gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt taattacgta
      901 agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt gactacacat
      961 tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg cagcggagca
     1021 acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag tcctcggcgc
     1081 tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc cttcaggttc
     1141 agaactctct ctgtatgata tcgctccagt gactcccggt gtggctgtcg atctgagcca
     1201 tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga
     1261 aggcgcagat gtcgttctta tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc
     1321 cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac
     1381 ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag ttgcgattgc
     1441 tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg gcgttaccac
     1501 gctggacatc attcgttcca acacttttgt tgcggaactg aaaggcaaac agccaggcga
     1561 agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc tgctgtcaca
     1621 ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac gcatccagaa
     1681 cgcgggtact gaagtggttg aagcgaaagc cggtggcggg tctgcaaccc tgtctatggg
     1741 ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt
     1801 tgtcgaatgt gcctacgttg aaggcgatgg tcagtacgcc cgtttcttct ctcaaccgct
     1861 gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga gcgcatttga
     1921 acagaacgcg ctggaaggta tgctggatac actcaagaaa gatatcgcct tgggcgaaga
     1981 gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga c
//
LOCUS       DAZPJP010000355         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPJP010000355 DAZPJP010000000
VERSION     DAZPJP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.358"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHU9J_004445"
     CDS             <1..389
                     /locus_tag="ACHU9J_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907798.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHU9J_004446"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHU9J_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1907799.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHU9J_004447"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHU9J_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1907800.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLAFYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHU9J_004448"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHU9J_004448"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHU9J_004449"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHU9J_004449"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          483 c          487 g          506 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gttttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPJP010000356         1971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPJP010000356 DAZPJP010000000
VERSION     DAZPJP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.359"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(219..1223)
                     /locus_tag="ACHU9J_004450"
                     /pseudo
     CDS             complement(219..1223)
                     /locus_tag="ACHU9J_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1208..1585
                     /locus_tag="ACHU9J_004451"
                     /pseudo
     CDS             1208..1585
                     /locus_tag="ACHU9J_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1624..>1971)
                     /locus_tag="ACHU9J_004452"
     CDS             complement(1624..>1971)
                     /locus_tag="ACHU9J_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1907801.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT          446 a          478 c          546 g          501 t
ORIGIN      
        1 aaaaaaagat aagcacaagt gattgattgg tcagatattg atcacttggt gtttgtattt
       61 tgaactttgc gatcaaaatt tgatctttgt tgtttccggt caatccttta caggttttct
      121 gtattatccg ggggagttaa gtctgtcgat gtccgacaga cttttgatga tggaaacaac
      181 atgcagcgat aatgtgcatg ctgttttgca ggtggaactc agaaggtgaa ctggaactgt
      241 gcgaccgcac cgtaagtgtt gtccgtgccg atcaggcctg caacatcaag gtgaaccaca
      301 tcaaacggtg agatccccac cccggccgtg aatgcactgc cgtcgttacc ggccagattc
      361 tgacggtaac cggcgcgcag ctgtgcccat ttccaggcat taaattctgc gccaatggcg
      421 gcaaactgac ggttgctgtc ggaggtgaaa ccgctggccg gggtcagatc cacatcaaat
      481 gcggtggtga acatcgcgtt gtgccaggag acaccggccg tcacctgcgg gcggatcctg
      541 aaggtttccg tgataccgtt cacttctttt gtctcaatac tacgggggat caggttctgt
      601 gcgaccagtc ccagcgtcca gttgtcatcc aggtccgtac tggcaccgat gtcggcgttg
      661 atgccatttt tcgtgttgtg gtagcggtcg cctttgaagg cgctgctgtc ataatttctg
      721 accagcacgt tgtagttgaa caggtcgacg cgctggaatt tgggggggaa gcccagagac
      781 catttctgcc cggccgtttc cagttccgta gccagagcaa tacccacatc agtgatcagt
      841 gccgcgcggg caaaggcctg tgatgtcagg cggcttttgt ccacctcctg cccggtgctg
      901 tttgccacct cttccaggta agtgaggtca gcatcagaga ctttaccgtc gacactgacg
      961 gtgccgtgtg atttaaccat cagtgcggca gagaggcgat caccgggaag ggccgccaca
     1021 gcagaaacgc ccagctgtgc ggcggcatgt gtgttacgga agtcccggag gtgttctttc
     1081 aggcgtgcgg cagcctcagg aacaccgtgg ttactttcga tggcgcggtc gaaggccttc
     1141 cactcatcac tgatccggtc ggcgttgtcg gtgatattat ccggattgga gagctgcgcg
     1201 ccgacggacg gaagcaggat gctgggctca tgcgcgacgc aaaatccacg atgtatatat
     1261 cagtactaaa agcgcgacgg cggaagaagc tctgaaacta atcggtgaac tgtacgccat
     1321 agagcacgat atacgcgggt taccggtgtc tgaacgcctg gcggtcaggc aaatgcagag
     1381 tagaccgctc ctgacttccc tgtataagct gatgcaggag aaagaacaca cgttatcgaa
     1441 gaaatgtcgt ctgggagatg cgttccggta tatcaggaag cactgggctg cgttgtgcaa
     1501 cttctgtgat gatggcctgg ctgaggtaga taataatgca gcagaaagag cgcttcgtgc
     1561 agtctgtctc gggaagaaaa ctttttcgag gatcactcat tccgtgtctg actgcttcag
     1621 ggtttaattt tgcttctgct gactgaatga tgattgggca gcaagtgcaa tatcccacat
     1681 aaatccagtt aactcacgag ccaccgccgt aatggctact tgtgattttt tcccttttgc
     1741 tgtcagagtt cggtaacgag aacacaatcg ttgttgagct ttccatgcaa tatctttgga
     1801 atattgtgtt acagagtccg gtcggtaata tataagccat gctccaactt ttgcaggtgt
     1861 acgataagac caagcggctt cgtaaagcag acgtctcagt tcgctgtttc caacttttgt
     1921 tattcctctg ggacgaatac tgtttccact ggaatattca cctggcacca g
//
LOCUS       DAZPJP010000357         1969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPJP010000357 DAZPJP010000000
VERSION     DAZPJP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.360"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            11..751
                     /locus_tag="ACHU9J_004453"
     CDS             11..751
                     /locus_tag="ACHU9J_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1907802.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            786..1969
                     /gene="htrE"
                     /locus_tag="ACHU9J_004454"
                     /pseudo
     CDS             786..1969
                     /gene="htrE"
                     /locus_tag="ACHU9J_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          557 a          424 c          436 g          552 t
ORIGIN      
        1 gggatggttt atgtttttta acaccaaaca tacaacagct ttatgcttcg taacctgtat
       61 ggcttttagt tcatcgtcta ttgcggacat tgtcatttcg ggtactcgcg taatatataa
      121 aagcgatcaa aaaagtgtca acgtacgtct ggaaaataaa gggaataacc cgttgcttgt
      181 ccagagttgg ttagatactg gcgataacaa cgctgagcct ggcagtatta cagtcccttt
      241 tactgccacg ccgccagtat cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat
      301 gtacacagcc agcacctcac tgcctaaaga cagagagagc gtgttctggt ttaacgtact
      361 ggaagttcca ccaaaaccag atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc
      421 atttcgcaca cgtataaaac ttttctatcg cccggatgga ttgaagggaa atccctctga
      481 agccccgtta gccctgaagt ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt
      541 gaccaatcca accccttact acgtctcttt tagcagtggt gatttagaag ctagcggtaa
      601 acgctatccg attgatgtga aaatgattgc accatttagt gatgaggtca tgaaagtcac
      661 tggccttaat ggcaaagcaa gttctgcaaa agtacatttt tacgccatta atgattttgg
      721 tggcgccatt gaaggtaatg ccagtctgta atcaggcaag gatataattc cgcaggaagc
      781 ataacgtgac tataaaacca actaaaaatt atcataatca tctgacccgt atcgctacgt
      841 tttgcgcgct gctgtattgc aatagtgctt tctgtgctga acttgttgaa tatgaccata
      901 ccttcctgat ggggcagaat gcctcaaata ttgatctcag ccggtatagt gaaggtaacc
      961 ctgctatacc gggtatgtac gacgtcagtg tttatgtcaa cgatcaacct attattaacc
     1021 aaagcattac atttattgaa attgaaggaa aaaaaacgca caagcctgta tcacacttaa
     1081 gaatttattg cagtttcata taaattctcc cgatataaat aacgaaaaag ccgttctgct
     1141 tgccagggat gaaacactcg gcaattgcct taatttgacg gaaattatcc ctcaggcttc
     1201 tgttcgttat gacgttaacg agcaacgtct ggatatagac gttcctcagg catgggtaat
     1261 gaaaaattac caaaactatg ttgatccatc gttatgggaa aatggcatta atgcggccat
     1321 gttgtcatac aacctcaacg gatatcatag tgaaactcct ggtcgaagaa atgacagcat
     1381 ttatgctgct tttaacggtg ggatgaattt aggtgcatgg cgactgcgtg cctcaggcaa
     1441 ctacaactgg atgaccgatt ctggcagtaa ttatgatttt aagaatcggt atatacagcg
     1501 tgatatcgcc tcgctgcgtt ctcaactcat tcttggtgag tcttatacaa cgggcgaaac
     1561 ctttgattct gtcagtatcc gcggcattcg tttatacagc gacagccgca tgttgcctcc
     1621 gacattagcg agttttgcgc ctatcattca tggcgttgct aataccaacg ccaaagtcac
     1681 tattacgcaa ggtggctata agatttatga aacgactgtg ccgccaggcg ctttcgtcat
     1741 tgatgatctg agtccgtcag gttacggcag cgatcttatt gttactgtcg aagaatccga
     1801 tggctcaaag cggacattct cgcaaccttt ttcatcggtc gttcaaatgt tacgccctgg
     1861 cgttggacgt tgggatatta gcggcggcca ggtcttaaaa gatgatattc aggatgagcc
     1921 taatttattt caggccagct actactacgg tctgaataac tatctaacg
//
LOCUS       DAZPJP010000358         1933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPJP010000358 DAZPJP010000000
VERSION     DAZPJP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.361"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            205..747
                     /locus_tag="ACHU9J_004455"
     CDS             205..747
                     /locus_tag="ACHU9J_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1907803.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            827..1501
                     /locus_tag="ACHU9J_004456"
     CDS             827..1501
                     /locus_tag="ACHU9J_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1907804.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1517..>1933
                     /locus_tag="ACHU9J_004457"
     CDS             1517..>1933
                     /locus_tag="ACHU9J_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1907805.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELES"
BASE COUNT          618 a          333 c          413 g          569 t
ORIGIN      
        1 tttacataaa tctacattaa ttgatagatg cgataaatag ttaatactct taaatatttg
       61 ttgtatttat attgcaatgg ttaatttgtt tcaaataaat aaaacaatgt gtttacattg
      121 cggtaacttt tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa
      181 aaaaatttta ttaaggaaca tattatgaaa cgttcaatta ttgctgccgc tgtcttttct
      241 tcttttttta tgagcgctgg agtatttgct gcagacgttg ataccggaac attaactatt
      301 aaggggaata ttgcagaatc tccgtgtaaa ttcgaagcgg gtggtgattc agtaagtatt
      361 aatatgccga ctgtaccaac cactgtcttt gaaggtaaag ctaaatattc tacctatgat
      421 gatgcagtcg gtgtaaccag cagcatgtta aaaattagct gcccgaaaga agttgctggt
      481 gtaaaactct cgttgattac caacgataaa ataaccggta acgataaggc gatagccagt
      541 agcaacgata ccgtgggtta ctatctctat ttaggtgata acagcgatgt cctggatgtt
      601 tctgcacctt ttaacattga gagttataaa acagcggaag gtcaatatgc tattccgttt
      661 aaagcaaaat acctgaaact gacagataac tcagtgcaat caggtgatgt gttatcttct
      721 ctggttatgc gtgtggcgca ggattaataa ataaaattgt aagtttctcc aggtaataaa
      781 catggccgct atcccatggc ggccttttaa tttaagaggc attaaaatga aaggattatt
      841 atctttactc attttttcta tggtccttcc tgcacatgcc ggaattgtta tctacgggac
      901 gcgcattatt tacccggcag aaaataaaga agtgatggtg cagttgatga accagggaaa
      961 ccgttcttcg ctgctgcagg cgtggattga tgatggcgat acgtcattac caccagaaaa
     1021 aattcaggtt cctttcatgt taacgccacc agtggcaaaa ataggggcaa attccgggca
     1081 gcaagtaaaa atcaaaatta tgccgaataa actgcccact aataaagaaa gcatttttta
     1141 tctgaatgtt ctggatattc caccaaatag tccagagcaa gaaggtaaga atgcactgaa
     1201 gtttgcgatg caaaacagaa ttaagttgtt ttaccgacca gcgggtattg ctccggtaaa
     1261 taaagcgaca tttaaaaaat tgctggtaaa tcgcagtggc aatggtttgg tgataaaaaa
     1321 tgactcagct aattgggtga cgatttcgga tgtcaaagct aataatgtca aagtcaatta
     1381 tgaaactatt atgattgccc ccttagaaag tcagagtgtt aatgtcaaaa gtaataatgc
     1441 aaataactgg catctgacca ttatcgatga ccatggcaac tatattagtg acaaaattta
     1501 attgcaggag ctgcctatgt tgagaatgac cccacttgca tcagcaatcg tagcgttatt
     1561 gctcggcatt gaagcttatg cagctgaaga aacctttgat acccatttta tgataggtgg
     1621 aatgaaagac cagcaggttg caaatattcg tcttgatgat aatcaaccct taccggggca
     1681 gtatgacatc gatatttatg tcaataagca atggcgcggg aaatatgaga ttattgttaa
     1741 agacaacccg caagaaacat gtttatcaag agaagttatc aagcggttag gcattaatag
     1801 cgataacttc gccagcggta agcaatgttt aacatttgag caacttgttc agggtgggag
     1861 ctatacctgg gatatcgggg tttttcgtct cgatttcagt gtcccgcagg cctgggtgga
     1921 agaactggaa agt
//
LOCUS       DAZPJP010000359         1919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPJP010000359 DAZPJP010000000
VERSION     DAZPJP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.362"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            62..613
                     /locus_tag="ACHU9J_004458"
     CDS             62..613
                     /locus_tag="ACHU9J_004458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907806.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            1364..1804
                     /locus_tag="ACHU9J_004459"
     CDS             1364..1804
                     /locus_tag="ACHU9J_004459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907807.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
BASE COUNT          578 a          319 c          456 g          566 t
ORIGIN      
        1 taattgccaa tgttttaaaa atgatcaatt atcaataact taaagcgtaa cgagcaaagt
       61 tatgaacatt gataattggg caataccgat aacaagtttg ggtggtcagt tgctgggggt
      121 tgagttggtc agccatgtag aacgcgaggg aatcaggcag atcggcctat gggaagaaga
      181 agtacttcat gggcaactaa tgcttattga aagcaaggcg gcatggttca gggataacgg
      241 tttgtattgt gtgattacta ctggccgtgg tgaaccatac gagtacttac cattccttcg
      301 gcagatgaca agggatatga aagtacataa ccagcagtgg cttgatgatc tcggtgtaca
      361 tggtggttct gctattcctc tggttacagg tgatttcgag gttgcccgat taaatagaag
      421 gtacacagaa gagaacattg accgtctgat ctttccagta ctcataaaaa gcattcgcca
      481 gtactgcgag aaggtgatcg tacccgtcag agataaaacg tattacccaa ttctcagaga
      541 ggccggtgta tgggcggtac agggagagtt caggccaatg cgttttgagc aatgtgaaaa
      601 attaatagga taacaataat gaaaaaatta ctaattgggt tactgattgc ccttagtact
      661 ggatgtacta ctacgcacag cttcatggat aaagcttacg ttggctatgt agatgacaag
      721 gccgtagtac tgtttgatgc aggaacacgt aatggggtga agtactaccg tcctcagttc
      781 cagagtgatt gcggtcgcat ctataacaag atctatcatg atgacaatac aagtactgga
      841 tgcctcatac gagattcccg cattgctgaa ctacatccct atagagagca tgtaagtaag
      901 gtagtaccac atcagaccga tagtgttcac gattggttag aaggggtcaa ttcaccatcc
      961 catatcctcg acaatctata gggtacgtat gggaaaggta atggtgattt tgttagtact
     1021 ggctggggta ctcttcaaca ttgctggtga ggatgcaccc cagccacagt acacggacaa
     1081 tgagaaccag aacgctatgg atttcgcaca gtcactaatc aagggtaagt acccatgcga
     1141 tatggtgact atggatagta ctgagaatgg tacaggacgt attgatgtac ggtgtgatgg
     1201 taagtaccag tatgagatca tccggcatga tggacaatgg caggtagtat cgaagtaatc
     1261 cgaaaagttt agtgcttagg ctgtatggtg agaatctaca gggctttttc tttatgcggt
     1321 gataagatcg gagtgaactt tttatgaagg aatagttagt agaatgaaaa ttacagtcga
     1381 taatatctct gaaatgtttg caggaactaa agaactacta atcggtggct taataacagg
     1441 tatcgtcgga gtacttattt taactcgtgt tagtggttat gaatacgtta aagtaccttt
     1501 aatgttagta atgatattct tactaactgt agttcctggt tttattgcta tgcttttttc
     1561 accactaacg aaagtatttg caaagatatt tgatattaaa agcagtaata tactttgccg
     1621 cattttctca tttctatatt tcatcttgta tatggctatt actttcttgg tgactaattt
     1681 tatattcact accgcaatgt cattacacgg cggggatggt gggttgagag catgttttgc
     1741 aattggctgt tttgtcgttg cagttgagca gacaagaaaa ttctatagtt tgcttatcaa
     1801 gtaaagggca agtactaaag aaatgtgtat ttgatgaagg gtaagtactt tcatcaaata
     1861 cgcaacaaat tacaaggtga gcatttcatc acaattgttt taattaacga tattttaaa
//
LOCUS       DAZPJP010000360         1881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPJP010000360 DAZPJP010000000
VERSION     DAZPJP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.363"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            197..700
                     /locus_tag="ACHU9J_004460"
     CDS             197..700
                     /locus_tag="ACHU9J_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1907808.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(779..1030)
                     /gene="yecJ"
                     /locus_tag="ACHU9J_004461"
     CDS             complement(779..1030)
                     /gene="yecJ"
                     /locus_tag="ACHU9J_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1907809.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1145..1231)
                     /gene="azuC"
                     /locus_tag="ACHU9J_004462"
     CDS             complement(1145..1231)
                     /gene="azuC"
                     /locus_tag="ACHU9J_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1907810.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1494..1817
                     /locus_tag="ACHU9J_004463"
     CDS             1494..1817
                     /locus_tag="ACHU9J_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1907811.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          493 a          439 c          413 g          536 t
ORIGIN      
        1 atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt
       61 cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga
      121 attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga
      181 tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa
      241 ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag
      301 tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat
      361 gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt
      421 tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga
      481 gcaacgttct agtacgttgg cacagttagc cgatgaagcg aaagaactga atgatgattc
      541 aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggtc tgttgctgca
      601 aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga
      661 ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg cgctaatgca
      721 ttgcgccgat gaaggttttg agaaaccgct gcctcatctg tttgaagcag cggttttttt
      781 aatgggattc accctgtggg gtaaacttga gttcaataag cgcgatggct ttttggattg
      841 cccgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagct
      901 gactggacat tttttcacga acttcaacgg gcgcgattac atcgagaaca tccagaattt
      961 gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg
     1021 gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat
     1081 cttcagcgat agtcacatcc accgtcatca ggacacaaaa aaacctgccg gagcaggttt
     1141 tttgttatcg gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga
     1201 acatactttt caggattttg cgcagtttca tcgcggcact ccgaccattt gttatacagg
     1261 tgttattgtc tttgcgctta taatatgaca accatcacaa aaatcaatct ttatgtgata
     1321 caaatcacat aaatacccct ttaatgttat aaaaatgata atcaaaaaac agccccccta
     1381 tttctgacac ctacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt
     1441 cgcagcgctg taacgcgtaa aatagtgctt tctcttactc ttctggctgg accatgagac
     1501 ttctgattct gactctttca ttaataacgc tggcggggtg tacggttact cgccaagccc
     1561 atgtgagcga agttgacgcg gcaacaggta ttgtacggtt ggtttatgac caggcttttt
     1621 tgcagcatgc ccataccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc
     1681 aggaaggcta tacccacgcg gtcccctttg gtcagccagt aggcaactgt agcctttttg
     1741 cgggttcctt atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct
     1801 tccctgtctt tctctaaata ttttaatcag cgaggggatc ttcgctgatt aaagaaatag
     1861 cggtaatgcg ttttattccc a
//
LOCUS       DAZPJP010000361         1868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPJP010000361 DAZPJP010000000
VERSION     DAZPJP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.364"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHU9J_004464"
     CDS             complement(<1..271)
                     /locus_tag="ACHU9J_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1907812.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..860)
                     /locus_tag="ACHU9J_004465"
     CDS             complement(315..860)
                     /locus_tag="ACHU9J_004465"
                     /inference="COORDINATES: protein motif:HMM:NF019114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1907813.1"
                     /translation="MDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVL
                     KPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTA
                     VRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIEDGNKQEE
                     INEDTEESTTDDTTAEDTENE"
     gene            896..1459
                     /locus_tag="ACHU9J_004466"
     CDS             896..1459
                     /locus_tag="ACHU9J_004466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907814.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1605..>1868)
                     /locus_tag="ACHU9J_004467"
     CDS             complement(1605..>1868)
                     /locus_tag="ACHU9J_004467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907815.1"
                     /translation="TSAAIPDFKCWDIHNDSFSTDMSFYVTRYLGKMPPPYGAFHYET
                     APDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
BASE COUNT          549 a          349 c          323 g          647 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccat cctctattac ttccatgtca
      421 tcgggtaact caaattccat tgagcctaat tggtataatg ttttatcaat atgcgttctt
      481 aatacatcgc gtaaatcctt tgcatctgat tgtgcaaata attcaagata agtttttgat
      541 ggtattgcac gcactgctgt tttgacctgg aatagatatt ccgttagtac ctgttctata
      601 tattcagtac tgaccaccag cccattcttt tctgctaatt ctaattctgc taactgccgt
      661 tctgctgaaa gcttcttcaa acgctcttgt tcaatttgtt cttttgtatt agtatcacgc
      721 aacggcttta gtacattatc tacaatccaa cttcttgcgt ttgaatcagt gtctgtcggc
      781 attccctttt ctacccaagt tcgaactgta gattcatcgt atccgtactg tctcgctaat
      841 gaccttaatg agatatccat atttgttcct ttttatttta tttatagggt ttacaatgtc
      901 agaccgtaac aatgattttt ctctgttagt aaaattgctg aacggatttt tgaagaaaga
      961 tgagataaaa aaaagactat attctttcgt gaaaaaagaa actaatgatt gggaaaagtg
     1021 gctgcaaata gaatttgctc attatctaac ttcaaaaagg aagtacgagc tacatcgtga
     1081 agttacgata tacctcgatc caatagatta tcctgatagt aaacttgcta aagtagattt
     1141 gatactgcgt gaaaaatcca caccctctga tgattatatt tttattgagt ttaaatgtac
     1201 taaaggtgta gtaccattag ttaggggctt aaatgctgac aaaaataaaa ttagtgctat
     1261 aagacagtgt aactacaaaa tgcgtaatta tcttggtgtc ggatttcatc ttttttgtga
     1321 accagatgat atcgagttta tggaaaagta cgtaactgaa gaacttaaag gctcttacgc
     1381 cgtgtttaaa ttatgcgagt gtccagtaag atataattgc caatgtgaat ttaatgaaat
     1441 tggtgtggta cttttttaaa tgcggtgcgg gtatgatcat tttgttcaca cacaattgat
     1501 taaacccgct gccgaaaacc ctcgatagta ccgccagtat ggagggaccc attgaatgca
     1561 atggttaata tactttgtaa gctatataca atgaatcatg agtatcaaag ccgcttacat
     1621 ttaatatcat cgtacaaagt tccttctttg tttagatatc tcgaaccgta attatatcca
     1681 tcatcaaatg cttcattggt tattaccagt tccctgtaaa atttctcatc aggtgctgtt
     1741 tcataatgaa atgcaccata aggcggcggc attttaccga gatatcttgt aacgtagaaa
     1801 ctcatgtcag tactaaatga atcgttatga atatcccagc atttaaagtc tggaatagct
     1861 gcacttgt
//
LOCUS       DAZPJP010000362         1841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPJP010000362 DAZPJP010000000
VERSION     DAZPJP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.365"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHU9J_004468"
     CDS             37..264
                     /locus_tag="ACHU9J_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1907816.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHU9J_004469"
     CDS             complement(265..639)
                     /locus_tag="ACHU9J_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR1907817.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHU9J_004470"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHU9J_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
BASE COUNT          457 a          466 c          458 g          460 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat t
//
LOCUS       DAZPJP010000363         1829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPJP010000363 DAZPJP010000000
VERSION     DAZPJP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.366"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="ACHU9J_004471"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="ACHU9J_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1907818.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHU9J_004472"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHU9J_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR1907819.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..>1829)
                     /gene="fimH"
                     /locus_tag="ACHU9J_004473"
     CDS             complement(1114..>1829)
                     /gene="fimH"
                     /locus_tag="ACHU9J_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1907820.1"
                     /translation="QLNDYLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVM
                     DSRLIFRLKVIRPFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCE
                     LNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEAS
                     AVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITG
                     QKPTEGPFSALGYLRVDYQ"
BASE COUNT          537 a          436 c          380 g          476 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgca
//
LOCUS       DAZPJP010000364         1770 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPJP010000364 DAZPJP010000000
VERSION     DAZPJP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.367"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            54..656
                     /locus_tag="ACHU9J_004474"
     CDS             54..656
                     /locus_tag="ACHU9J_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR1907821.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
     gene            complement(951..>1770)
                     /locus_tag="ACHU9J_004475"
     CDS             complement(951..>1770)
                     /locus_tag="ACHU9J_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR1907822.1"
                     /translation="RLASRFDRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERYT
                     YIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIILL
                     NSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFDK
                     VTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLWT
                     TWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          512 a          409 c          376 g          473 t
ORIGIN      
        1 ataactctct atttataaag aaaaatcagc aaaaacttgt ttttgcgtag tgtgtggtgc
       61 ttttggtggt gagaaccacc aacctgttga gcctttttgt ggagtgggtt aaattattta
      121 cggataaagt caccagaggt gggaaaatga aaaaatggat gttagccatc tgcctgatgt
      181 ttataaatga gatctgccag gccactgatt gctttgatct tgcaggccgg gattacaaaa
      241 tagatcctga tttactgaga gcaatatcat ggaaagaatc ccgttaccgg gttaatgcca
      301 tcggtattaa tccggtaacg ggatatggca gcggactgat gcaggtagat tcccagcatt
      361 ttaacgaact ggcccgctat ggaattaagc cggaacatct gacaacagat ccctgcatga
      421 acatttatac cggtgcttat tatctggcaa tagcctttaa aaaatggggc gtctcctggg
      481 aggccgttgg tgcatacaat gccggattca ggaagaccga acgccagaac cagagacgtc
      541 ttgcctacgc atcagaggtt taccggattt ataccgggat aaaaagcagt aaaggtatcc
      601 ggattccggt cacgaagaaa tcacttccgg aaattaacag cgtgcagaac aattaatctt
      661 acagacattg tcagataccg gttatgccgc aaaagcggca tgaagtccac acacaaaaat
      721 atcatatgag tgacaatgct gactggccga agccccgcga agcgggaatc catcttatat
      781 atacccggta actgcgcagc ctcctgccag accatttttc gcgggcacaa tatttacaga
      841 caaccggaaa gccggttgat ggaattgagg tatatcagac gacacagcga tgccgaagaa
      901 tgataatata agtaaagccc cgaaaaattt tcggggcttt acttatatta tcatgacttc
      961 cactttctga actgttcggc aatcacccat aacgccttgt tgattcttat atcaccatca
     1021 atacctgtaa tggctcttgt cctggtattt ttcccggagg cacttcgccc cgataatcca
     1081 ccttttatca tattctcctg aacccgctgc caggttgtcc agagatcgtt ctgtttatct
     1141 tcccgacgac ggggagtaat tatttgttca ggcgtcactg gcgttttatt ttcatcttca
     1201 tacctgacca tcagtgcagc tctgccaaat aaatgttgct cgtcactgtt aagatgaatt
     1261 tctttcatcg cctccatatt atcagtgacc ttatcaaaga caccgagcac ttcatacgct
     1321 ccctcgataa cctgcccgac aatatcacct ttatgtggaa cacggatttc gccaaaatta
     1381 tttccgcaca ccaggccatt tgtgcagaca aaacgaaaaa ttccggggat catctgataa
     1441 ctggatgaac catcatgtga attaagcagg ataatttcag gaacttcctg cccgttaata
     1501 tgcccttccc tgcgaagacg taacatatgt ttactgtatt cgcggcgtcc caaatcacga
     1561 acccgactct gacaggcaaa gaatggctgg aaaccttcat cacgtaactt attgatgata
     1621 ttgattgttg gaatatacgt ataacgttca ctccgggact catgtttatc accggaaaat
     1681 accgaaggca cgaactgcat taattcatca tccgttaaag gacgttcacg acggatggaa
     1741 ttataccgat caaaacggga agctaatcgc
//
LOCUS       DAZPJP010000365         1728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPJP010000365 DAZPJP010000000
VERSION     DAZPJP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.368"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHU9J_004476"
     CDS             complement(<1..356)
                     /locus_tag="ACHU9J_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1907823.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(356..850)
                     /locus_tag="ACHU9J_004477"
     CDS             complement(356..850)
                     /locus_tag="ACHU9J_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR1907824.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1208..1384
                     /locus_tag="ACHU9J_004478"
     CDS             1208..1384
                     /locus_tag="ACHU9J_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907825.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1526..1678)
                     /locus_tag="ACHU9J_004479"
     CDS             complement(1526..1678)
                     /locus_tag="ACHU9J_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907826.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1666..>1728)
                     /locus_tag="ACHU9J_004480"
     CDS             complement(1666..>1728)
                     /locus_tag="ACHU9J_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1907827.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          476 c          399 g          412 t
ORIGIN      
        1 ctggcgagtc gttttttgga gtgttcgcgg gctttttcct ccgtcatctg aataatcagc
       61 atatcagcag gatcgcaaat cacgttgtaa atcacccagc cgtcaatcag gccgatagtc
      121 ttgccggttc gtgccgggcc aacaaatatc actgcgtcgt attcacgcga ggccaggcag
      181 ttcatcggct caataacata cggtgccacc agcggatccc acgggactga gttccctgcc
      241 cccatgggca cccgcatata ctgagcaacg gcatcagcaa cccgcattcg tctcggtgcg
      301 cgaaggatat aacctgaatc ggttcgtgct gcctttgcgg tttcctgatt cagcattact
      361 cctcctgctg taattcctcc tcatcatccg cacctgcttc agtcacccgc agggctatct
      421 gatcgcgcag atcatcaata atggactgaa cacggctcac agcggcaggc tgcagaccgc
      481 agtcacgttc aagaatatcc ggtaatgtct ccagcacctg cacgaccgct tttgcccaga
      541 tggcaaactc ccgtctgaca tcactggccg gaatgagttg tgccgtttcc tgttcgaact
      601 taagacgctc acgttcagac tgataccagg ctttgcgctc atgcgcgtcc atttcgcctt
      661 ctgcaaccgg cggtggtaat gccagaaatg ccgacacaat atcaaccacc cgataaagct
      721 tgaggttgct ttcatgcccc cctgcaacgg gtagattttg cagccttgcc gcagcagtct
      781 ggcgatgtac acctgacagt gccgccagtt gactgatatt cagcgtcaga ttttttaact
      841 ctcgatccat acccgctcca gaatgtttta aacatgcatc ttgcgaacaa ctttaggcaa
      901 acggtgttag tgatgaacaa aaaacaatca aaatcgacac cataaaaata aaaccactgt
      961 aatatcaatc cattacagta gtggtgatga cgaatgaaat ttcaaaaact agcctttttc
     1021 cgcgccgctc ccgccccgtg gcaggccacc ccaccgggag gacccgtcag cctgacagcc
     1081 atgacgaacg tctgatacag cgccctgcat gaatgacatc gggataatcc agaaaggaat
     1141 agcatcgtac ccacaagaat cagtgtaagt gtcctgtttc ttccaccccc gcacaggact
     1201 ggcgagcatg agggacaaac ccgcgaacca taaacgcggt aaaaacccgg tgtgcatcgt
     1261 ttttgattat tcccgcacac tcgcgcagaa ggagttcccc gtcgggctac ggtcatggtt
     1321 aatacgggaa tacggcgacg atacagcgca tgatgtgtca ggcttgaata cctttatccg
     1381 ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc aaagccactc
     1441 tgtaggaagt ggcttttgta atggcaataa aaagccccgc gaatgcgagg ctaaatcctg
     1501 gtatttgtaa tgactggctc ttatctcaac gcagcccctt accgcgcgca aaatgctcaa
     1561 tatcaagcat cagcaatgag atgtttaatc tggattcact ccagaagtga gcaccaccct
     1621 gtctacagag ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg
     1681 cgtcctgatg tattcctgaa gcgttctcag tgctgtttgg tcgcggat
//
LOCUS       DAZPJP010000366         1726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPJP010000366 DAZPJP010000000
VERSION     DAZPJP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.369"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHU9J_004481"
     CDS             complement(<1..959)
                     /locus_tag="ACHU9J_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1907828.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..>1726)
                     /locus_tag="ACHU9J_004482"
     CDS             complement(1560..>1726)
                     /locus_tag="ACHU9J_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1907829.1"
                     /translation="RRISKRYDDVEVIVKATSNDGLSVTRTADKDSAKTFVQETLKDT
                     WESADEWFVH"
BASE COUNT          448 a          294 c          425 g          559 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcggg
//
LOCUS       DAZPJP010000367         1703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPJP010000367 DAZPJP010000000
VERSION     DAZPJP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.370"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            22..129
                     /locus_tag="ACHU9J_004483"
     CDS             22..129
                     /locus_tag="ACHU9J_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907830.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            335..1618
                     /gene="gltA"
                     /locus_tag="ACHU9J_004484"
     CDS             335..1618
                     /gene="gltA"
                     /locus_tag="ACHU9J_004484"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1907831.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          443 a          412 c          402 g          446 t
ORIGIN      
        1 cattgccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt
       61 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
      121 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
      181 cctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
      241 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
      301 agtcttacgc aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
      361 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
      421 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
      481 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
      541 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctggaag tttgttacat
      601 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
      661 ccgtcatacc atgatccacg agcagattac ccgtctgttc catgctttcc gtcgcgactc
      721 gcatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
      781 gctggatgtt aacaatcctc gtcaccgtga aattgccgcg ttccgcctgc tgtcgaaaat
      841 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
      901 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
      961 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
     1021 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
     1081 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
     1141 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
     1201 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
     1261 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
     1321 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
     1381 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
     1441 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
     1501 cgttggctgg atcgcccact ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc
     1561 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
     1621 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
     1681 tgcccaaaca caaaaaattg taa
//
LOCUS       DAZPJP010000368         1700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPJP010000368 DAZPJP010000000
VERSION     DAZPJP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.371"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..483
                     /gene="cas6e"
                     /locus_tag="ACHU9J_004485"
     CDS             <1..483
                     /gene="cas6e"
                     /locus_tag="ACHU9J_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1907832.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
     gene            480..1403
                     /gene="cas1e"
                     /locus_tag="ACHU9J_004486"
                     /pseudo
     CDS             480..1403
                     /gene="cas1e"
                     /locus_tag="ACHU9J_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHU9J_004487"
     CDS             1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHU9J_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1907833.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT          402 a          394 c          493 g          411 t
ORIGIN      
        1 cgcttttttg tcctgtcgca ggaacgcccg gcggaaagtg atattttcac catcgaatgc
       61 cgaccatttg cgccagagtt gcgtaccgga caatcccttt gtttcaacct gcgggcgaat
      121 ccaacaattt gtaaagcggg caaacgccac gacttgctga tggaggcgaa acggcaggtg
      181 agggggcagg tggaaggaag tgatgtctgg ttgcatcaac aacaggctgc gctggactgg
      241 ctggcagcgc agggggagag aagcggtttt acactgctgg atacttcggt tgatgcctac
      301 cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag tagcgtggat
      361 tatacgggga tgcttacggt caccgctcct gggttatttc tgcaacgtct cagtcagggg
      421 tatggcaaaa gccgggcgtt cggttgtggt ctgatgctga tcaaacccgg agcagaggcg
      481 tgacgtttgt accgctgagc ccgatcccat taaaagatcg cacttccatg atcttcctcc
      541 agtacggtca aatcgacgta ctggacggcg ctttcgtgct gatcgacaaa accgggatcc
      601 gcacgcatat tccagtggga tcggtcgcct gcattatgct cgaaccggga acaagagttt
      661 cccacgcggc agtccatctg gccgccacgg tgggaacact gctggtctgg gtcggtgaag
      721 cgggcgttcg cgtttactcc tccggacaac ccggaggggc gcgggcagat aaattactct
      781 actaggcaaa gctggcttta acggaagatc tacggttgaa ggtggtgcgc aaaatgtatg
      841 aattacgttt tcgtgaacca ccgccagctc gccgttcagt ggagcagtta cgtggaattg
      901 agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt gtgaaatgga
      961 atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat cgctgcatca
     1021 gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc gcgggatatg
     1081 cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat gacatagccg
     1141 atatcattaa atttgattcg gttgtgccaa aggcatttga aatagcagcg aggcaacccg
     1201 cagaacctga taaagaagtc agattagcct gtcgcgatat tttccgtagc actaagttaa
     1261 cgggcaaatt aataccgtta attgaggaag tccttgctgc aggtgaaatt gaaccaccac
     1321 aacccgcgcc ggatatgtta ccgcctgcca tccctgaacc tgaaacgctg ggtgatagtg
     1381 gtcaccgggg gcgcggcgga tgagcatggt cgtggttgtt acagaaaatg tccctccgcg
     1441 cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt atgttggtga
     1501 tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg ctggttgcgg
     1561 aaatgtggtg atggcctggg cgaccaatat cgagtcgggt tttgaatttc agacctgggg
     1621 agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt ttcttcctgt
     1681 tgataatcaa taagttatcc
//
LOCUS       DAZPJP010000369         1657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPJP010000369 DAZPJP010000000
VERSION     DAZPJP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.372"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            211..>1657
                     /gene="istA"
                     /locus_tag="ACHU9J_004488"
     CDS             211..>1657
                     /gene="istA"
                     /locus_tag="ACHU9J_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR1907834.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          419 a          463 c          414 g          361 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc ggacagacgc
     1621 tttatcgaca atctgctgcg tcatcatcgg gataacg
//
LOCUS       DAZPJP010000370         1645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPJP010000370 DAZPJP010000000
VERSION     DAZPJP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.373"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            46..1299
                     /locus_tag="ACHU9J_004489"
     CDS             46..1299
                     /locus_tag="ACHU9J_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907835.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1348..1467)
                     /locus_tag="ACHU9J_004490"
     CDS             complement(1348..1467)
                     /locus_tag="ACHU9J_004490"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1907836.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          337 a          417 c          406 g          485 t
ORIGIN      
        1 ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag ccgtactaca
       61 actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca gccaaatcaa
      121 tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt ctctgccgga
      181 ctggcaacct ttgcactcct ctattgtgtg cagcctatcc ttccggtgct ttcgcaggag
      241 tttggcttaa cccccgcgaa cagtagtatt tcactgtcca tttccacggc gatgttggct
      301 attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc agtgatggtc
      361 acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac cagctggcac
      421 ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc agctgttggc
      481 atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat ggggttgtat
      541 atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt cttcacggac
      601 tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc ctcggcgttg
      661 atgttctgga aaatccttcc ggaatcacgc cattttcgcc cgacttcgct gcgccctaag
      721 acgttattta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt attgttcgca
      781 gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg ctatcggttg
      841 atgctttccc cctggcatgt cagtcaggcc gtggttggct tattatcgct ggcttatttg
      901 accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg gcgcggtccg
      961 gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt attcagctcg
     1021 ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc agcccactca
     1081 gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc ctcctcgctg
     1141 tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg tgttttctgg
     1201 cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt cattgctctg
     1261 ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc ggactgcggt
     1321 aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc ctaaaataga
     1381 cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa gtacccaaat
     1441 aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc taccgctttg
     1501 taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa atttccctta
     1561 agtcacaata cgttattatc aatgctgtaa tttattcagc gtttgtacat atcgttacac
     1621 gctgaaacca accactggaa gtctg
//
LOCUS       DAZPJP010000371         1633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPJP010000371 DAZPJP010000000
VERSION     DAZPJP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.374"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..595
                     /locus_tag="ACHU9J_004491"
     CDS             <1..595
                     /locus_tag="ACHU9J_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1907837.1"
                     /translation="EKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAM
                     SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRY
                     YISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAI
                     NILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            606..1043
                     /locus_tag="ACHU9J_004492"
                     /pseudo
     CDS             606..1043
                     /locus_tag="ACHU9J_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1043..1420
                     /locus_tag="ACHU9J_004493"
     CDS             1043..1420
                     /locus_tag="ACHU9J_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907838.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          545 a          247 c          420 g          421 t
ORIGIN      
        1 agagaagata caaaaacagg gaggtgatta tttattcgcg gtaaaaggaa accaggggcg
       61 gctaaataaa gcctttgagg aaaaatttcc gctgaaagaa ttaaataatc cagagcatga
      121 cagttacgca atgagtgaaa agagtcacgg cagagaagaa atccgtcttc atattgtttg
      181 cgatgtccct gatgaactta ttgatttcac gtttgaatgg aaaggactga agaaattatg
      241 cgtggcagtc tcctttcggt caataatagc agaacaaaag aaagagccag aaatgacggt
      301 cagatattat atcagttctg ctgatttaac cgcagaaaag ttcgccacag caatccgaaa
      361 ccactggcac gtggagaata agctgcactg gcgtctggac gtggtaatga atgaagacga
      421 ctgcaaaata agaagaggaa acgccgcaga attattttca gggatacggc acatcgctat
      481 taatatttta acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc
      541 agccatggat agaaactatc tcgcgtcagt ccttgcgggg agcgggcttt cgtaatcttt
      601 ccctagggag tcagccgaat gttgggaagg atttgacgga tgaagagaag aactgcttgg
      661 ccggcggcag tacgggaagc ccgggaggat ggggaccaga agatgagaat agcgcaaggg
      721 atcgtatgca gcatgaacgg tataaagatg aattgcgaca gaaaatggga aaacccgacg
      781 ttacagattc aaatttgaaa aatattatag acgatcttta tcggccaaat gcaaaagtag
      841 gaagtggaag cacagcagat gctgttcgtt atgaattggc cacaggtgaa aaagttggtg
      901 gtagaggaca tgtattgaaa gcacaggatt atacaagggc tttacaggat tggttggata
      961 agaatccaac agcatctcca ggggatcgag cagcagcaga aaacgttctt caggatttac
     1021 agaatgcttt gggaggcaag taatgaacca atatattgct agttatatag aaaaaatggc
     1081 atcattttcc actgttttgt ctgagacagt ctcattagct aatgaatact ggcatccaga
     1141 aacgccaccg gtggttatgt tgttctctca ggttggaaaa acgattgtaa aaaattttta
     1201 tgaaatgggg gatattgaaa gagaaaaaat atttaaacat gtcgaagatg gtatggtctc
     1261 gcttgatgat gaattggcca cagttgtagc tacagggctt atagaggcaa tagtcactgc
     1321 gaccgatagc aattctttac tttggcaaga tttaagtaaa tttctgggtg acaaatcaaa
     1381 gtctcatgca ttagtgtgga aaaattttgg tcaggagtga tattattcaa gaagtattaa
     1441 gctcgctatt gagatcttaa tgcttcttga gattaaatta aagttaggac gaagtaattg
     1501 caggtggaaa taaatccata gtgacgtgtg gcaatggcta ttagatgtta tggtgtgatg
     1561 ggctttggtg atttaagagc taagttagat tgccttatga aaaagtatgg ttatatattt
     1621 aatgatgtgt atc
//
LOCUS       DAZPJP010000372         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPJP010000372 DAZPJP010000000
VERSION     DAZPJP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.375"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHU9J_004494"
     CDS             <1..94
                     /locus_tag="ACHU9J_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1907839.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            105..293
                     /gene="dicB"
                     /locus_tag="ACHU9J_004495"
     CDS             105..293
                     /gene="dicB"
                     /locus_tag="ACHU9J_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1907840.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            290..481
                     /locus_tag="ACHU9J_004496"
     CDS             290..481
                     /locus_tag="ACHU9J_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1907841.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            575..>1630
                     /locus_tag="ACHU9J_004497"
     CDS             575..>1630
                     /locus_tag="ACHU9J_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR1907842.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          362 c          391 g          379 t
ORIGIN      
        1 ccggttttat ctggaagaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
       61 gactgtagag caattactca aaattcattg ttgaggacgc gataatggaa acgttattac
      121 caaacgttaa tacgactgaa ggttgttttg aaattggtgt cactatcagt aacacggtat
      181 ttactgaaga tgccattaac aagagaaaac acgaacggga gtcattaaat aaaatatgca
      241 ttgtttcaat gctggctcgt ttgcgtctga tgcaaaaagg atgctggcga tgaatacagc
      301 atttgcactc gttctgaccg ttattcttaa tacgggcgaa ccagctgaac tggttaccgg
      361 agtatacaac tccatgaaag aatgcgtggc tgctgcagct gaacagaaaa ttcccggtaa
      421 ctgttatccg gttgataaaa tcattcacca ggataataaa gaagtcccgg caggtcttta
      481 aagcagccac gtaataaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      541 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      601 cattccggac gaaaaggcaa caaaagaaga aggcgctaaa cctgtagcca ctgccattga
      661 agccggcgac gaacgccgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      721 ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg gtataccccg
      781 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      841 tgcttccttt gtcccggttg agactgaatc cgaaccgatg aacgtcactt ttgacaagct
      901 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      961 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
     1021 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtgtccgg agattaaact
     1081 gcatgccatc gggtggatta agcataaatg taagcctggt gcaaaatggc cagaaattca
     1141 ggcagagatg cgcatctgga aaaaatgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
     1201 cacgtctgtt gttgatctcg cccgtgccag agccaatcaa cagcacactg aaaattcaac
     1261 aggaaaaatc agcccggcca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
     1321 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
     1381 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
     1441 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1501 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1561 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1621 cgcaactgat
//
LOCUS       DAZPJP010000373         1615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPJP010000373 DAZPJP010000000
VERSION     DAZPJP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.376"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            76..840
                     /gene="nfeF"
                     /locus_tag="ACHU9J_004498"
                     /gene_synonym="yqjH"
     CDS             76..840
                     /gene="nfeF"
                     /locus_tag="ACHU9J_004498"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1907843.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(894..969)
                     /locus_tag="ACHU9J_004499"
     tRNA            complement(894..969)
                     /locus_tag="ACHU9J_004499"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(934..936),aa:Ile,seq:cat)
     gene            1094..1600
                     /gene="mug"
                     /locus_tag="ACHU9J_004500"
     CDS             1094..1600
                     /gene="mug"
                     /locus_tag="ACHU9J_004500"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1907844.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          387 a          397 c          445 g          386 t
ORIGIN      
        1 tatcatgatt aacaatcatt atcatttacg aaaattattt agatatatct gattaccatc
       61 acgaaggcga taacaatgaa taactccccc cgctacccgc agcgcgttcg caatgatctg
      121 cgcttccgtg aactgactgt gttacgcgtt gagcgcatca gcgccggttt tcagcgcatt
      181 gtcctcggcg gcgaggcgct ggacggtttt acatcgcgcg gctttgacga tcacagcaaa
      241 ctcttctttc ctcaacctga cgctcacttt gtgccgccaa cggtaacgga agagggcatc
      301 gtctggccgg aaggaccacg cccaccgtcg cgtgactata cgccgctgta tgacgaacta
      361 cgccatgaac tggcgatcga tttctttatt cacgacggtg gggtcgccag cggctgggcg
      421 atgcaggcgc aaccgggcga taaacttacg gttgcaggtc cgcgcggttc gctggtggtg
      481 ccggaagatt acgcgtatca gctgtatgtc tgcgatgaat ccggaatgcc cgcactgcgc
      541 cgccgcttgg aaacgttgag caaacttgcc gttaaaccgc aagttagtgc gctggttagc
      601 gtgcgggata acgcctgtca ggattatctc gcgcaccttg atggttttaa tatcgaatgg
      661 ctggcacatg atgagcaggc ggtagatgcg cgtctggcgc agatgcaaat ccctgccgat
      721 gattacttca tctggataac cggcgaaggt aaagtcgtta agaatttaag ccgccgcttt
      781 gaagcggaac agtatgaccc acagcgggta cgtgcagcgg cttactggca cgcaaagtaa
      841 ctgatatata aaaagaaaaa ggctgacgat ttctcgtcag cctttgctat atctggtggc
      901 ccctgctgga cttgaaccag cgaccaagcg attatgagtc gcctgctcta accactgagc
      961 taaggggcca tggtagcgga ttataaagta actccgtgtc gcaatccagc cattaccgtg
     1021 cgcctgctgt ttttataaac aatgtatttt caatccgtta tacttttcct ggtgatgtta
     1081 gaaaggagta aatatggttg aggatatttt ggctccaggg ttacgggtcg tgttttgcgg
     1141 tatcaaccct gggctttcat ccgccgggac tggttttccc tttgctcatc cagcaaatcg
     1201 cttctggaag gtgatatatc aggccgggtt taccgaccgt cagttgaagc cgcaggaggc
     1261 acagcatctg ctggattatc gttgtggcgt caccaaactg gtagaccgtc caacggtgca
     1321 agccaatgaa gtttcaaagc aggagctgca cgcaggtggg cgtaagctga ttgaaaaaat
     1381 tgaagattat cagccgcagg cgttggcgat tctgggcaaa caagcatatg aacagggatt
     1441 cagccagcgc ggtgcacagt gggggaaaca aacgctcacc attggttcga cgcagatttg
     1501 ggtgctgcca aatcccagcg gtttaagtcg cgtttcacta gagaaactgg ttgaagcgta
     1561 tcgcgagctg gaccaggcgc tggtagtgcg tgggcgataa aaaacgccac cgaaa
//
LOCUS       DAZPJP010000374         1553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPJP010000374 DAZPJP010000000
VERSION     DAZPJP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.377"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            153..1196
                     /locus_tag="ACHU9J_004501"
     CDS             153..1196
                     /locus_tag="ACHU9J_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1907845.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          438 a          348 c          335 g          432 t
ORIGIN      
        1 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
       61 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
      121 actatatcac cacatgtttt acaggctcag aaatgaacga tagattatgc tttgaagtgc
      181 acgataacaa gggttatttc gtattttcgg atacctggtt tggcccattg ctgggggagt
      241 ttgaagaagt gcttgatgca tacgacgctg atgaaatcag cgagacgagt tacatcaaca
      301 aactccgacg ccttgctcaa cgggagccgg actttattga tatacacgct catctggcat
      361 atgcatttct ggaacagaat gccccgcgta aggcactgaa cgcctctctt aaaggactgg
      421 cggcaggtaa tcgtatcatc ccggaaagtt tctgtggtga aataatctgg atgcatcccg
      481 aaaaccggcc ttatctgcgc gcgctttatg cagcaattct cgcaaatgtg cacttacagc
      541 gccatcagga tgcggttatg ctcactgata aaatactggc atacaatcct gaagataatc
      601 agggggctcg ctggttgttg ggttcagagc tgttacgcac cggcgatcat gagcgagcct
      661 tcagtgtact gaaagagcat gctgatgaat tttcacctta ctggtatgaa ctcggtctcc
      721 tgcattttct gaacggggaa catgttaagg ccgccacagc ttttcgccat ggctttgcca
      781 caaacactta catcgcagaa atgctgtgcg gaaatctgca tccatttcca cttgctgtgt
      841 ggcatgactt ctccggctct ctggatacgg cagaagatta ctatgcaaca tactcaccat
      901 tatggggaca atatccggaa gcattgctgt ttgtaaactg gctctataac cattcctccg
      961 tgctgcatga acgatcagaa atcattaaat gcgccgaaat gctgattcag gaagatgatt
     1021 tcgaaatatg cgaaagtatt ttaagacagc aggagcacct gtggaaaaga attgacaaga
     1081 cactttctga ggaaattgta cagaaatgca gaaatatgaa cggtgaatat atctggccct
     1141 ggatattgcc cttttccgca gcagggatga aacattcaag catccagtat cagtagatat
     1201 tgcattagtg taactttaaa acaagtaata atttattatc caataataac actgtgttcc
     1261 ttccccaggt gattaccctg gggttatttt ctgaaagttt cctttgcaaa aggatagcgt
     1321 tgtaaattac tttaacgaat tactccatga actcattaaa gttctttcgt aatactttat
     1381 cgtcaaacac gttatagctg gcatcaaaaa ccacagattt accctgccag cgggtaatcc
     1441 cctcctctgg cgttttcggt tgaactggta tccccgtgga tttgccccta tatttccaga
     1501 catctgttat cacttaaccc attacaagcc cgctgccgca gatattcccg tgg
//
LOCUS       DAZPJP010000375         1549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPJP010000375 DAZPJP010000000
VERSION     DAZPJP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.378"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHU9J_004502"
     CDS             351..476
                     /locus_tag="ACHU9J_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR1907846.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHU9J_004503"
     CDS             complement(921..1397)
                     /locus_tag="ACHU9J_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1907847.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          358 a          405 c          386 g          400 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcgccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 aacgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZPJP010000376         1547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPJP010000376 DAZPJP010000000
VERSION     DAZPJP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.379"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            89..532
                     /gene="cmtB"
                     /locus_tag="ACHU9J_004504"
     CDS             89..532
                     /gene="cmtB"
                     /locus_tag="ACHU9J_004504"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1907848.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            560..>1547
                     /gene="cmtA"
                     /locus_tag="ACHU9J_004505"
     CDS             560..>1547
                     /gene="cmtA"
                     /locus_tag="ACHU9J_004505"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1907849.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          358 a          345 c          390 g          454 t
ORIGIN      
        1 tttccctgct caattgcttt cattaaaccg cacgagtatt ttatttttac cgccggacgg
       61 taccctaaaa ataaaataag gataatttat gcggcttagt gactattttc cagaatcatc
      121 aatctcagtt atacattcag caaaagattg gcaggaagct atcgatttct cgatggtatc
      181 attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag attccaccat
      241 taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg cgcgaccgga
      301 atgtggggcg cttaaaaccg ggatgtcttt gacattactt gaacaaggtg tttattttcc
      361 ggggaatgac gaaccaataa aattactcat cggcctttct gctgccgatg ccgattcgca
      421 cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac tcgaacaact
      481 cttaacagca gcatcagaaa aacaattagc ggacataatc agccgcggat aattactctc
      541 tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc aggcttttgg
      601 gggctttttg acggcaatgg tcatccccaa tattggtgct tttattgcct ggggttttat
      661 tactgcgtta tttattccca ccggttggct gcctaacgaa catttcgcca aaattgtcgg
      721 cccgatgatt acctatttat tgcccgtgat gattggttct accggtggtc atctggtcgg
      781 cggtaaacgc ggcgcggtca tgggcggaat aggtactatt ggtgtgatcg tcggcgcaga
      841 gatcccgatg ttccttggct caatgattat ggggccgctc ggtgggctgg tcataaaata
      901 tgttgataag gcactggaaa aacgcatacc tgccggtttt gagatggtta tcaataactt
      961 ctcattaggt atcgcgggga tgctcctttg tctgctgggt tttgaagtta tcggcccggc
     1021 ggtgttaatt gccaatactc tcgtcaaaga gtgtattgag gcgctggtac atgcgggtta
     1081 tctgcctctg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctca ataatgcgat
     1141 cgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg gtaaatccat
     1201 cttctttatg gtggcgtcca atcctgggcc tggcttaggg ctgctgctgg cgtttacctt
     1261 gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc acttcctcgg
     1321 tgggatccac gaactgtatt tcccgtatgt gctgatgaag ccgctgacca ttattgccat
     1381 gattgcgggc ggtatgtctg gcacctgggt gtttaactta ctggacggtg gtctggtggc
     1441 tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag gctcgttcct
     1501 ggcgacaatt gccggtgtta cggtagctac cctggtgtcc tttgcta
//
LOCUS       DAZPJP010000377         1537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPJP010000377 DAZPJP010000000
VERSION     DAZPJP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.380"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>1537
                     /locus_tag="ACHU9J_004506"
     CDS             <1..>1537
                     /locus_tag="ACHU9J_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1907850.1"
                     /translation="YEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDE
                     DDFKKPVRVNLPVVGDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTD
                     NTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTELHEARKNIPPK
                     NPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENKVRQTEDKQHQNSE
                     PEER"
BASE COUNT          440 a          400 c          372 g          325 t
ORIGIN      
        1 attacgaagc aaaaaatgac aaagtaagaa aacgcctcgg tattaaaggc ggtttttact
       61 gggctgaagc gaaaaaatta tccattgcca tctcccgtgg tgctgttgcg attgacgatg
      121 ctggctacga tgaagatgac tttaaaaaac ctgttcgcgt caatttgccc gttgttggtg
      181 acctcccacc agaaggcgtg tttgatacgg aattctgcaa ccgttacgaa aaaggcgggg
      241 aagatggcat cacaatggta tttatcgcat cttctccctc tgttcaggac aaaccagcca
      301 gcactgacaa taccaacgtc aacggcgaag acatgacaga aattgaggag aacatgctcc
      361 tgccagtttc cggtcaggaa ctgcccattc gctggcttgc tcaacacggc agcgaaaaac
      421 cggtaacgca cgtttcacgc gacggactcc aggcattaca cattgctcgg gctgaagaac
      481 taccggctgt tactgccctg gctgtttccc acaaaaccag cctgctcgac ccgctggaaa
      541 ttcgcgatct ccacaaactg gttcgtgaca ctgacaaagt tttccctaat cctggtaatt
      601 caagcctggg actgataact gcttttttcg aagcatacct gaacgctgac tacaccgatc
      661 gaggactgct gacaaaagag tggatgaagg gtaatcgtgt ttcacacatc actcgcacgg
      721 cttccggtgc taatgctggc ggcggaaacc tcaccgatcg cggcgaaggt ttcgtacacg
      781 atctgacgtc actggcgcgc gacgtagcca ctggcgtact ggcccgttca atggatctgg
      841 acatctataa ccttcatccg gcacacgcta aacgcattga ggaaattatc gctgaaaata
      901 aaccgccctt ttctgttttc cgcgacaaat tcatcaccat gcctggcggg ctggattatt
      961 cccgcgccat cgtggttgcg tccgtaaaag aagcaccaat tgggatcgag gtcatccccg
     1021 cgcacgtcac tgaatatctg aacaaggtac tgactgaaac cgatcatgcc aaccctgatc
     1081 cggaaatcgt ggatattgcc tgcggtcgct cctctgcccc gatgccgcag cgtgtaacag
     1141 aagaaggaaa acaggatgat gaagaaaaac cgcaaccatc ttgcgcaatg gcagatgaac
     1201 aggcaacggc tgaaacagtg gaaccggatg caactgaaca tcatcaggac acgcagccgc
     1261 tggatgctca gtcacaggta aatgctgttg atgcgaaata tcagaaactg cggacagaac
     1321 tccatgaagc ccggaaaaac attccgccca aaaatcctgt cgatgcagac aaattactgg
     1381 ctgcctctcg cggagaattt gttgaaggga ttagcgaccc gaatgattcg aaatggattc
     1441 ccggtcatca tatctcctca aatgaagtca aaaaaacgga aaataaagtg cgacagactg
     1501 aagataaaca gcaccagaac agcgaaccgg aggagcg
//
LOCUS       DAZPJP010000378         1529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPJP010000378 DAZPJP010000000
VERSION     DAZPJP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.381"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..937)
                     /locus_tag="ACHU9J_004507"
     CDS             complement(<1..937)
                     /locus_tag="ACHU9J_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1907851.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQ"
     gene            complement(963..>1529)
                     /gene="rfaY"
                     /locus_tag="ACHU9J_004508"
     CDS             complement(963..>1529)
                     /gene="rfaY"
                     /locus_tag="ACHU9J_004508"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1907852.1"
                     /translation="NDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLI
                     VETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEI
                     KASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIA
                     NEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          473 a          323 c          265 g          468 t
ORIGIN      
        1 gttgatattt cacttgatac tgcttttcat tcgtggctgg aataaacgcc acatcattcc
       61 agcaagaagc ctgataagcc tcattaaaat acttctggca gggataattc gtccagtcgt
      121 tccagggctt cgttacgcca acataatgaa tcagcttagt atcatcggta atggtctttt
      181 tataatcttc atcgctttta ttttttaact cataatcgat gccataaaag caatcataat
      241 cgccgctaat aaaaatacaa tgaccaaaga acagaatatt taaaatatcc tgatcaaggc
      301 atttatactt cgtgtgctgc gggtcacttg ccagcatagc aatggcttta ttcataaaat
      361 catttttcgc ccatgcttca agctgcagat atactacacc ggcgttgaaa taattccctg
      421 gaagtccctc tatagccaga cgcttcgctg gtttttcctg cataaaaggc acatctttaa
      481 taacagctgc aaacttatcc ccatcaaaat taatatcaag caatccagct aatgagcctt
      541 tacagatgac atcagcatcg atatataaca gattttcaat agtttcactc agatactgga
      601 acgcaactaa acggaagaat gttgcataag accactgttt caggctgggg aagatatcaa
      661 taaaggcact ggagacttta taaattctta cttcaacgtt agcatttttg ctggtaaaag
      721 cattaacacg ttgtagataa tcttcatcaa tgtagtcggt aaagagatga aacttaaccg
      781 caaggtccgg attatgcata acaactgatt gcatagatac tgccgcacca aataaaaagt
      841 tacggtcaat accgtaagcg atatttaaca cacgatcggt gttagcaatt tccccagaga
      901 cagcgacttc agtaatactc tcagctaata aatccatgtt ggttccgttt tgactgtgtg
      961 gtttacgctt tgccttttaa ttttttaata aatttgcgta gtttggttct gtagattact
     1021 gaatagtagc cataatcttt tatttcgttg gcaataccca ggtgacgttc catggccaga
     1081 cgatcacgag ctttacgctc tgccgtacag cttttaccgg ataggtcaat tatcctgaca
     1141 ccatctttac taacgataaa gttaccccgg tgtgggtcgc ccgatagcat atttaaggca
     1201 tgcaattttt ccattgaagc cttaatttct gctttaacat cttctggaat gatcggcata
     1261 tcattgagct ctacgccctc gacatactcg ataagcatga taaaaacact ggcatagttg
     1321 aaaattttac gctcagccag aaaataaaaa tcattaggaa aagtaagacc tgcacttctg
     1381 acacgatcgg tttcaacgat caaattctga tagtagtcac cttttacaaa agattttaaa
     1441 aaacgttcat tccttttcgt tttcggggca aagactttaa aaacatagcg accatatttc
     1501 gtatcaatca atgatacttt ggtatcatt
//
LOCUS       DAZPJP010000379         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPJP010000379 DAZPJP010000000
VERSION     DAZPJP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.382"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHU9J_004509"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHU9J_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHU9J_004510"
     CDS             complement(397..954)
                     /locus_tag="ACHU9J_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1907853.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHU9J_004511"
     CDS             complement(951..1226)
                     /locus_tag="ACHU9J_004511"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1907854.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHU9J_004512"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHU9J_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZPJP010000380         1521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPJP010000380 DAZPJP010000000
VERSION     DAZPJP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.383"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            57..719
                     /locus_tag="ACHU9J_004513"
     CDS             57..719
                     /locus_tag="ACHU9J_004513"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1907855.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1083..1521
                     /locus_tag="ACHU9J_004514"
                     /pseudo
     CDS             1083..1521
                     /locus_tag="ACHU9J_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          478 a          301 c          300 g          442 t
ORIGIN      
        1 tcttaaaaga gagtaaacct tgtcaagagt gattgcaata aaaattttta tttcttatgt
       61 caattgagca tattttaatc gggaagcatc atgggggttc tgattactgg agaactccat
      121 ttttgttatt taagaactta caccgtgaat ttattttcag tctagatggt gctgcaactg
      181 agcatgacac gctgttacca cgattcaccg atgatataag ccgtcaatca tgggttagtg
      241 aaaaggtctt ctgtaacccg ccgtacagcg atattccgtc gttcctctta aaagcgtctg
      301 aagcggattt agtagctttc ctgataccgc atagagccaa caccagctac tggcttaggc
      361 acatttacag caataaccac tgtcatgaaa tccgtattct tcatcgtgca gttaaatacc
      421 ttccaccagc agggcataac aggcttacta ttaggtcacc gtttccaagt gcagtcgttg
      481 tttttaaaaa agaaccacgt aagcatgaaa ttacacagat ggtttgctgt gctgatacat
      541 tgctaccgtt aaccatcatt aaccgtggtg gactaagagg aagacccacc atttacccac
      601 ctgagacgct cgacagcttc ataaaactat acaggcaagg aaaacccatt aagtacattg
      661 cagatgctct cagaatgcct ctgagcacgt catacaggat tgctcaaagg ttgagctaaa
      721 attctcataa atatggtcat ttacaagaca ggtgattcag atgttaagaa gtatccctgt
      781 gaacaataaa tgaccaatca gtaatcattc tgtgaaataa ttgttgcaat ttataatcac
      841 atctgttata gtttctatat gggctgataa aaaagttcat gtcttgaagt ggtaacactt
      901 ggggaggtgt cagatggttg gcgtatatta acctctcatt cagggtatac ctcagcgttt
      961 taatcatcgt actgtttcag gcatggactt ctttatcagt ccatcaatcc cccagatatt
     1021 ttaatcacac aggctaatcg gttttatccg gttggccttt tcacgtatga ggtaatttat
     1081 gaatgtctaa atataaccca aatcagtttt ataaatttga tgtgcgaatc tctcaacttt
     1141 cagaagaaat taacgcagca gcaagattga tttatatggt catggtccat agctgtgact
     1201 tctatgaaga gaaatacaaa tcatattcac caagtattga ccatctcgct tatatggctg
     1261 gtgtatcaaa gagaacagca atcagagcca ttgagacact tgagaaacac ggtttaattc
     1321 aggtgcaaaa gaatcagggg cgtcaaagca cttatcgtgt attcacactg gatgataagc
     1381 cggaattact tgtaagacct acatcacagg aagttgcagc ctcagtaaga caaaatgaca
     1441 ccactaccag tgacaaaatg tcatgggggt agtgacaaaa tgtcacccgt gttagagaga
     1501 taagttatta gataaaacat t
//
LOCUS       DAZPJP010000381         1508 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPJP010000381 DAZPJP010000000
VERSION     DAZPJP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.384"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..181
                     /locus_tag="ACHU9J_004515"
     CDS             <1..181
                     /locus_tag="ACHU9J_004515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907856.1"
                     /translation="KIVGDVVVSYSRLMSSGINPLHCEYVKHQKKMNQIYEKSKYTYQ
                     GRKHDITLIPLWRIF"
     gene            247..>1508
                     /gene="ipaHb"
                     /locus_tag="ACHU9J_004516"
     CDS             247..>1508
                     /gene="ipaHb"
                     /locus_tag="ACHU9J_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR1907857.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
BASE COUNT          460 a          357 c          260 g          431 t
ORIGIN      
        1 taaaatagtt ggagatgttg tagtatctta ctcaagacta atgtcttctg gtataaatcc
       61 tctgcattgt gaatatgtaa aacatcaaaa aaagatgaac caaatatacg aaaaaagcaa
      121 atatacttac cagggtagaa agcatgatat aacactcatt cccttatggc gaatattctg
      181 aatcaccctc ctcattagga gagatacatt aaatacaatt tattcacaat ctaattccat
      241 atccatatgc gagaaattaa tatgctcaga aatatttcat cctgtttatt tccacatatc
      301 agcacaatta catcccccaa ccattatttg tccgaatggg atgattggga gaaacagggg
      361 ttaccggaag aacagcgtac tgaggcggta agaagacttc gtgcatgtct tacctctaag
      421 gggcataaac tggacctgcg agccttggcg ctttcctcgt tacctgtact ccctgcttgc
      481 attaaaaagc ttgatgtgag ctgtaataaa ttaaccatcc ttactgattt acctgaaaat
      541 attaaagaac ttattgcaag agataatttc ttaacacata tatctgcatt accacattat
      601 ctaataactt tggatgtgtc cgaaaatcaa ttagagaatc tgccgttatt accagacacc
      661 atcaaatcac taagcgcaga gtataatagg ttatccacac tgccttcatt acccttgaat
      721 ttaaaaaaac ttgaggttag gaacaacgaa ctgcaaactc ttccatctct gccttctaat
      781 cttaagatac ttaaggttgc gcacaaccat cttactgaac tgcccccttt acctaggaga
      841 ctgcaacttc tttttgcata tagcaataga ttaagcaact taccaaacat ccaagaaaat
      901 attatcatga gaagattttt ttattttgaa aacaaccaaa taactacaat cccgacaaat
      961 ctttttcgtt tagatcctca tataactatt gagattgcaa ataacccctt atcagatcaa
     1021 actctgctat tcttaataca gcaaacttcg gttccaaatt ttaacgggcc tcagtttcgt
     1081 atttccctgt cagaccaaaa cagactgttt ttacgccaga tgttgccgca aaatttacat
     1141 tcgcgccata tcagagtcat cactgaaggg gggcagaact ttcagatccc ccctcttccc
     1201 gaaactgtgg cagcctggtt tcctgaagca gatcgtcggg aggtttctac acaatggact
     1261 tctttttcca ccgaggagaa ttcccgggca ttctccgcgt tccttgaccg cctttccgat
     1321 accgtctctg cacgcaatac ctccggattc cgtgaacagg tcgctgcatg gctggaaaaa
     1381 ctcagtgcct ctgcggagct tcgacagcag tctttcactg ttgctgctga tgccactgag
     1441 agctgtgagg accgtgtcgc gctcacatgg aacaatctcc ggaaaaccct cctggtccat
     1501 caggcatc
//
LOCUS       DAZPJP010000382         1499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPJP010000382 DAZPJP010000000
VERSION     DAZPJP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.385"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHU9J_004517"
     CDS             complement(<1..346)
                     /locus_tag="ACHU9J_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907858.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHU9J_004518"
     CDS             complement(349..1059)
                     /locus_tag="ACHU9J_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907859.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..1323)
                     /locus_tag="ACHU9J_004519"
                     /pseudo
     CDS             complement(1075..1323)
                     /locus_tag="ACHU9J_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            1380..>1499
                     /locus_tag="ACHU9J_004520"
     CDS             1380..>1499
                     /locus_tag="ACHU9J_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1907860.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQ"
BASE COUNT          385 a          360 c          342 g          412 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
     1321 ggtgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     1381 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
     1441 gcaaaagcac tgccggacat cagcgctatc tctgctctca ctgccgtaaa acatggcaa
//
LOCUS       DAZPJP010000383         1482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPJP010000383 DAZPJP010000000
VERSION     DAZPJP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.386"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHU9J_004521"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHU9J_004521"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1907861.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHU9J_004522"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHU9J_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          324 c          371 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZPJP010000384         1433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPJP010000384 DAZPJP010000000
VERSION     DAZPJP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.387"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1226
                     /locus_tag="ACHU9J_004523"
     CDS             <1..1226
                     /locus_tag="ACHU9J_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1907862.1"
                     /translation="IPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
BASE COUNT          385 a          303 c          281 g          464 t
ORIGIN      
        1 tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca
       61 tttttctttt catgttaatg aaatatttca ctaacaaaca aattttacca acactcattt
      121 taagccttgc atttttaagt ggccttatct atttagttga aaccattgta attatccata
      181 aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata
      241 ttttccgcca actcagtttt atttgtttaa cctcgctggc actcttttgt tatggaaaag
      301 acaacatcct tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg ctgattcctt
      361 ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt
      421 atgtcgtcga ctactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa
      481 tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
      541 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
      601 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
      661 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcagaaa ctgcaactct
      721 ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca
      781 acagcgtaga gtcattattg tcgagacctg ttgttaagga cttctgtgtc atgctggttg
      841 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
      901 tggtttcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac
      961 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
     1021 ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
     1081 ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac acgcgtaata
     1141 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
     1201 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
     1261 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     1321 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     1381 cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cgg
//
LOCUS       DAZPJP010000385         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPJP010000385 DAZPJP010000000
VERSION     DAZPJP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.388"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(112..765)
                     /gene="ribB"
                     /locus_tag="ACHU9J_004524"
     CDS             complement(112..765)
                     /gene="ribB"
                     /locus_tag="ACHU9J_004524"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1907863.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(869..1017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1139..>1428
                     /locus_tag="ACHU9J_004525"
     CDS             1139..>1428
                     /locus_tag="ACHU9J_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFR1907864.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          396 a          380 c          335 g          317 t
ORIGIN      
        1 ttctcataga aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa
       61 gccagctaca acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc
      121 tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt
      181 gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt
      241 cagctcacac agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc
      301 ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg
      361 gcccgggcga ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt
      421 aatacgatca gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt
      481 aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt
      541 acggcgatct tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc
      601 catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc
      661 ttcatcatca agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac
      721 acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca
      781 ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata
      841 acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc
      901 ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc
      961 tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga
     1021 ttcactataa cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat
     1081 gacttaccct tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat
     1141 gattgacccg aaaaaaattg agcaaatcgc tcgccaagtt cacgaatcaa tgcctaaagg
     1201 aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct
     1261 gacgcgcctc gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg
     1321 gactcgtgag aaactggcgc ggcttgaaca aagaatgagc gaactggaaa atcgcagtac
     1381 tgagattaaa aagcagccag atccagagac tctcccccca acgctgta
//
LOCUS       DAZPJP010000386         1427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPJP010000386 DAZPJP010000000
VERSION     DAZPJP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.389"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHU9J_004526"
     CDS             complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHU9J_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1907865.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHU9J_004527"
     CDS             complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHU9J_004527"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1907866.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(823..>1427)
                     /locus_tag="ACHU9J_004528"
     CDS             complement(823..>1427)
                     /locus_tag="ACHU9J_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1907867.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          311 t
ORIGIN      
        1 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
       61 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
      121 gttaaactcc ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt
      181 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
      241 attcaggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
      301 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
      361 ggcgtattcc acggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc
      421 gcggcttcgg cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat
      481 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
      541 gggcgttcga tttatgaaaa ttcggtgagc cgggtccgcc agcttcgaca tactgttcat
      601 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
      661 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
      721 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
      781 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggtcgt catcatgcca cctcctgagt
      841 cagcgcaggg agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
      901 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
      961 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaatgtcc ccatattgat
     1021 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtgtcagtac cggagcgggt
     1081 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
     1141 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
     1201 ataagagcca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg
     1261 ggcgaaagcc gtgggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
     1321 agcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
     1381 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgcctt
//
LOCUS       DAZPJP010000387         1421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPJP010000387 DAZPJP010000000
VERSION     DAZPJP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.390"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHU9J_004529"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHU9J_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR1907868.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZPJP010000388         1416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPJP010000388 DAZPJP010000000
VERSION     DAZPJP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.391"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(313..>1416)
                     /locus_tag="ACHU9J_004530"
     CDS             complement(313..>1416)
                     /locus_tag="ACHU9J_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1907869.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          370 a          382 c          324 g          340 t
ORIGIN      
        1 aaatattttt taaatgtgac tgtcatcctt cagataataa agccatcatc gcaatagatg
       61 gaaaagcact cctgctcttt tttgattaga gccgtcgcag gaggaacaac tcatatcatc
      121 attgcgatct cgacgatgta tagtctcatc ctgcggcaga acaagacgga taaaaaatcc
      181 aatgagagca cactcatcca gaacttatta acataatgaa tattaaagaa aaatcatcgc
      241 agccaatgaa gtaatatgca aaaataatta gcccttgcag ggagcaaagt tccataatct
      301 cccccttccc cctcaatgat ccaaatacaa ataatgcatc caactggtca ttcttagcag
      361 caccttccgc atcaccgaaa catggctgaa atggtcggag taaactgcta cctgggcgta
      421 aatgccgtcg ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac
      481 gccgtccgtg ccgggaacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc
      541 gcctggcacg acaggtaaaa tactagtcaa tttgccgtgg aacacctgcc caggtagcgc
      601 gttaaacacc acttccgcat catcgccagg tttcagacgt aacagcgagt tttgccgaaa
      661 ttgggcgaca atttgccgtt tttgctcggg gatgaagacc atcaccggac gcagcggcaa
      721 ggcagctgcg tatgtacctg ggcggatcag tacctgagta acgtagccat tgctcggcgc
      781 gcgaatgaca gtctgctcaa ggttatattt tgcttcagta agttgcgctc ttaagctcac
      841 aatctgcgat tgctcgccgt taaccatact gtcgagctgg ctctggatct gcgcctgctc
      901 cgccaccgag cctttcacca gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat
      961 gtcacgttcc gagaacggat tcaccgccgc ctggctgcct ttcaggtaac gttgataatt
     1021 tttaaacaga cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttcagtgag
     1081 ctgcgcacgc agcgtcttta tattatgcgt cgccgtcatc aggtcagcct gaagtctgtc
     1141 aactcgcgcc tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag
     1201 ctgattattc ttgtcagtga cttcagtaac aattcccgtc acctgtggcg tgatagggat
     1261 cgctatcact gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat
     1321 caaaccactc accagaaaca cgcctcccag cgccgccgtc gccagcgtcc actgattcac
     1381 cggaatacga aagattttaa agaccgccca cgccag
//
LOCUS       DAZPJP010000389         1415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPJP010000389 DAZPJP010000000
VERSION     DAZPJP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.392"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1407
                     /locus_tag="ACHU9J_004531"
     CDS             <1..1407
                     /locus_tag="ACHU9J_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1907870.1"
                     /translation="NELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADP
                     QAEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLI
                     ALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLI
                     NQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQI
                     VQFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKP
                     LTRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIR
                     EQASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQE
                     LNEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAV
                     KQYLSHPVNTYTGVLLPK"
BASE COUNT          422 a          328 c          325 g          340 t
ORIGIN      
        1 aatgagcttg ccgccaaaaa agcattgtct cttgcggcta tcctggcgct ggaaaaagag
       61 tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc gttaacagta
      121 gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa tctgacatca
      181 ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga agagcaaaag
      241 ctacaaatta ctgccgtatc taataaaggc gatttaagtt tccctgcgca gcaaaaatca
      301 cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg ttggtgagct ctcctcttcc
      361 agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt cagtggcatg
      421 aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt tcagttgatt
      481 aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct acaaaatgct
      541 caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc ccagcagatg
      601 tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca gattgtacag
      661 tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc agcggatatc
      721 acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg tggcgttaat tacgcgttac
      781 ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt aactcgcgcg
      841 acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca ggtttcacag
      901 tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc gctcgatgct
      961 tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc atctggagca
     1021 tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag tcatttgctg
     1081 gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttagcgaa tgaagtgatg
     1141 gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatacca gcaaaacgtt
     1201 cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac tattgtaaat
     1261 agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt gttgaaacaa
     1321 atgacggtag agaatgttaa cactgccgtt aaacaatatc tttctcatcc ggtaaatact
     1381 tatactggag tattattgcc aaaataataa cagcc
//
LOCUS       DAZPJP010000390         1401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPJP010000390 DAZPJP010000000
VERSION     DAZPJP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.393"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            79..>1401
                     /locus_tag="ACHU9J_004532"
     CDS             79..>1401
                     /locus_tag="ACHU9J_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR1907871.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNG"
BASE COUNT          466 a          262 c          277 g          396 t
ORIGIN      
        1 ggtaagaatc atgctttgat aaccaattga taactattat tatattaatc taacaccatt
       61 gacatagttg aggtttgaat gaacaaaaag ctgtatttat tcccagactc aaacattttt
      121 ctacaatgca aggacttgac gcaggtaaat ttttccgaga ttactgcttg tgatgaagta
      181 tgtatcatta ttacaaggcc cattcagcaa gagattgacc gccagaaagg gcaaggtaat
      241 tcccgcctat ccaaaaaagc aaggaaggct gctagcctat ttaatcaagt agttgattca
      301 actgacatga ctcttgttat tcgtgaacgc tcgccgcgag tttttcttac catggatcta
      361 agcctgaaac catgtgaaca gttatctgaa caattggatt atcaggaggc cgacgatcgt
      421 tttgttggta ttgcggcagg gtattgcgct gacgatccag attcggaggt ggcaatcatt
      481 accaatgata gcggtccacg tttttcagca ataaaacata atataatctg ctataaagtg
      541 ccttcttcat ggttgcttcc tgctgagcca gatgaaaagg ataaacaaat taaattgctg
      601 gaagctaagc tgaagcaatt cactcagtct atgcccgatt tcagcattaa agtagccgat
      661 gacgagcaat taaatattgt acttccttat tatacagctt tatctgaata tgaagttgat
      721 aatttgcttt gtcaactaac ggatgcattc ccattggtta caaactttga caatgaacaa
      781 actaccccac aatcactaga aaaaatcatc aagtcattta gtcaacagga gtatttccct
      841 gtatcagcag atgatatttc tctttataaa gaaaaacatt atccactttg gaaaaagcag
      901 tgtgaagaaa tactgaaaaa ctgtcatact catattaatc cgaaggcaat aaattacccg
      961 ctgtcgtttt tattacaaaa cacaggttat actatcgctg aacgtgctgt ggtaacattt
     1021 acagcaaaag gagattttcg tctgtgtgga tttaattggg agataatgga agagctgact
     1081 gaaagcaaaa ggattgacct accttcagcc cctaccgccc ccaaaggaaa atggcgtagt
     1141 gtatgtggcg taacagaaag aatgccttac gctgggggag ctttggaaac accgctatcc
     1201 agtataattt caaaagaaat tggtcctttg ttatctctta ctccaacaga aagggataaa
     1261 aacgattttt attataaaga aaagagaaat gagccagttc aagttgtatc gctggaatgc
     1321 gaagagtggc gtcatcaagt caaagaagaa atattcccgt tgttcgtata tacagccaac
     1381 aaaccaaaga aagtgaatgg t
//
LOCUS       DAZPJP010000391         1386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPJP010000391 DAZPJP010000000
VERSION     DAZPJP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.394"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHU9J_004533"
     CDS             complement(468..1019)
                     /locus_tag="ACHU9J_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1907872.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT          400 a          312 c          256 g          418 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgata
//
LOCUS       DAZPJP010000392         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPJP010000392 DAZPJP010000000
VERSION     DAZPJP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.395"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHU9J_004534"
     CDS             complement(<1..1339)
                     /locus_tag="ACHU9J_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1907873.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPJP010000393         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPJP010000393 DAZPJP010000000
VERSION     DAZPJP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.396"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHU9J_004535"
     CDS             <1..984
                     /locus_tag="ACHU9J_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1907874.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHU9J_004536"
     CDS             999..>1377
                     /locus_tag="ACHU9J_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1907875.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZPJP010000394         1364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPJP010000394 DAZPJP010000000
VERSION     DAZPJP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.397"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHU9J_004537"
     CDS             complement(<1..1259)
                     /locus_tag="ACHU9J_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1907876.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          379 a          348 c          305 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAZPJP010000395         1359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPJP010000395 DAZPJP010000000
VERSION     DAZPJP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.398"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..348)
                     /locus_tag="ACHU9J_004538"
     CDS             complement(<1..348)
                     /locus_tag="ACHU9J_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1907877.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHU9J_004539"
     CDS             complement(569..1300)
                     /gene="bluR"
                     /locus_tag="ACHU9J_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR1907878.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          350 a          311 c          304 g          394 t
ORIGIN      
        1 ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc caacggaaaa cgcccgcgcc
       61 tgccagtgtt gaacagtatt cccgaaatgt agattttccg taaatatgaa gcaatgcggc
      121 ctcttttatt ttagcagggt tcttggtcgt actaactttt aacaggttcc tggttcctct
      181 taataacaaa accgtgtcat cgtgagtaat aattctgatg ttatccgtag ccagataata
      241 aatgtaatgt gcaatacggt gatgttttaa ttctgaataa aaccaggaga agttttgctc
      301 ttttctcact tgctcaaaca tcttttgaaa aacaacgacc tgatccatca ggataataac
      361 ctcttgttag ttgtgagact gcgtagtgtg cacgatcggt tttaccactt caatctggtc
      421 tgtcctttgg ctgtgatatg tacagagtgt gatagaggga ttacctgaat tcttccggtg
      481 agcattttgc aacggaccag ctccggtaca aatgctgttg tgggttcaga ttataacatt
      541 ctgtctaagg ggcgggataa aggtgaaatt agggggcatg aaagatgact ttataacctt
      601 gctcacccca atgttgtaaa agttcgtttt gccttctcgt tggtgccatg cctgtccaga
      661 caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga ttcaatggga tcagcaagaa
      721 cagaaatgtg ccatcctgac aatgataaac gccatgcttc cagccacaat cgtgctctct
      781 cttcaacatt ccacgccatc agaatggctt ctttaccggg cttacggcgc atttcgaaaa
      841 gtgaggctgc tgcatattca attaatgcgc cgtcaaacat actgctcata atgcgggagg
      901 tgttgtgatc aagcacgaga cgctggcgaa caggaaggta aacatgatta atcaattgat
      961 ctactgggta ctctcgaccc agtgaaataa ttctcgcgcg tagtttggca ggattagcca
     1021 tacgaagaat tgacatcatc tcttcttgca ggcggttcca gtcatcttcc gtatcctggc
     1081 tggtggtttc cagtaatgct ttaactttgc ctacagggac gccattactt atccaacgct
     1141 tgatctcttc gatgcgttgt atgtcttctt catcaaagag tcggtgtccg ccttcactgc
     1201 gctgtggttt taacaaaccg tagcggcgtt gccaggcccg gagagtgaca ggattaatcc
     1261 cgcaacgttc agcaacatca ccaatgctgt aataagccac aattcctcct tgcggtcata
     1321 aatctccgcc gcctgtacac gacccaataa tactttgta
//
LOCUS       DAZPJP010000396         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPJP010000396 DAZPJP010000000
VERSION     DAZPJP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.399"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHU9J_004540"
     CDS             <1..184
                     /locus_tag="ACHU9J_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907879.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHU9J_004541"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHU9J_004541"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1907880.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPJP010000397         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPJP010000397 DAZPJP010000000
VERSION     DAZPJP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.400"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            132..1338
                     /locus_tag="ACHU9J_004542"
                     /pseudo
     CDS             132..1338
                     /locus_tag="ACHU9J_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
BASE COUNT          282 a          418 c          358 g          283 t
ORIGIN      
        1 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
       61 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      121 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      181 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      241 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      301 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      361 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      421 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      481 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      541 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      601 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      661 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      721 cttcgctggg cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct
      781 cgtctgggta aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc
      841 gttcccggca cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg
      901 ctgcgtaagc aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct
      961 ctttacccgg tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc
     1021 accgaggtcg cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt
     1081 gcgccggtaa ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt
     1141 aataaagctc tcaaacgggc gttgttcctg tcggccttcg ccgcgctcag ggatccgctc
     1201 tccagggctt actacacccg caaaatgagt cagggaaaac gacacaatca ggcgcttatc
     1261 gccctggcga gacgacgctg cgacgttctg ttcgccatga tgcgcgacgg gactttttat
     1321 accccgcagg ggtcataaca t
//
LOCUS       DAZPJP010000398         1332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPJP010000398 DAZPJP010000000
VERSION     DAZPJP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.401"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            373..828
                     /gene="csgB"
                     /locus_tag="ACHU9J_004543"
     CDS             373..828
                     /gene="csgB"
                     /locus_tag="ACHU9J_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1907881.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            869..1321
                     /gene="csgA"
                     /locus_tag="ACHU9J_004544"
     CDS             869..1321
                     /gene="csgA"
                     /locus_tag="ACHU9J_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1907882.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          397 a          263 c          318 g          354 t
ORIGIN      
        1 gttttttctc cacacctccg tggacaaatt tttactccaa aaagacgagg tttgtcacgg
       61 cttgtgcgca agacatatcg cagcaatcag cgatgggcaa gaagaatgac tgtctggtga
      121 tttttgatag cggaaacgga gatttaaaag aaaacaaaat attttttgcg tagataacag
      181 cgtatttacg tggcttttaa tactttggta tgaacaaaaa aagaaaaata caacgcgcgg
      241 gtgagttatt aaaaatattt ccgcagacat actttccatc gtaacgcagc gttaacaaaa
      301 tacaggttgc gttaacaacc aagttgaaat gatttaattt cttaaatgta cgaccaggtc
      361 cagggtgaca acatgaaaaa caaattgtta tttatgatgt taacaatact gggtgcgcct
      421 gggattgcag ccgcagcagg ttatgattta gctaattcag aatataactt cgcggtaaat
      481 gaattgagta agtcttcatt taatcaggca gccataattg gtcaagctgg gactaataat
      541 agtactcagt tacggcaggg aggctcaaaa cttttggcgg ttgttgcgca agaaggtagt
      601 agcaaccggg caaagattga ccagacagga gattataacc ttgcatatat tgatcaggcg
      661 ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg gtaatactgc gatgattatc
      721 cagaaaggtt ctggtaataa agcaaatatt acacagtatg gtactcaaaa aacggcaatt
      781 gtagtgcaga gacagtcgca aatggctatt cgcgtgacac aacgttaatt tccattcgac
      841 ttttaaatca atccgatggg ggttttacat gaaactttta aaagtagcag caattgcagc
      901 aatcgtattc tccggtagcg ctctggcagg tgttgttcct cagtacggcg gcggtaacca
      961 tggtggtggc ggtaataata gcggcccaaa ttctgagctg aacatttacc agtacggtgg
     1021 cggtaactct gcacttgctc tgcaaactga tgcccgtaac tctgacttga ctattaccca
     1081 gcatggcggc ggtaatggtg cagatgttgg tcagggctcg gatgacagct caatcgatct
     1141 gacccaacgt ggcttcggta acagcgctac tcttgatcag tggaacggca aaaattctga
     1201 aatgacggtt aaacagttcg gtggtggcaa cggtgctgca gttgaccaga ctgcatctaa
     1261 ctcctccgtc aacgtgactc aggttggctt tggtaacaac gcgaccgctc atcagtacta
     1321 atacatcatt tg
//
LOCUS       DAZPJP010000399         1299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPJP010000399 DAZPJP010000000
VERSION     DAZPJP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.402"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHU9J_004545"
     CDS             <1..1117
                     /locus_tag="ACHU9J_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1907883.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..>1299
                     /locus_tag="ACHU9J_004546"
     CDS             1095..>1299
                     /locus_tag="ACHU9J_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907884.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAZPJP010000400         1269 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPJP010000400 DAZPJP010000000
VERSION     DAZPJP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.403"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(155..793)
                     /locus_tag="ACHU9J_004547"
     CDS             complement(155..793)
                     /locus_tag="ACHU9J_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1907885.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            complement(811..>1269)
                     /locus_tag="ACHU9J_004548"
     CDS             complement(811..>1269)
                     /locus_tag="ACHU9J_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1907886.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
BASE COUNT          332 a          288 c          259 g          390 t
ORIGIN      
        1 tgacttagat gtaagtcgta atgttatata catggcgtat tttattctta ctggttctac
       61 acatgttaat cgcatcggat tgatcagttc accttaataa gtttaataaa tcgtactgca
      121 catagtacat tacgatggta actatttaat tggtttacat gaataatggc ttgtcaccat
      181 cagtcacggg ttgattgata cctatattct caccaatgtg tttctcaggt ttatcgatga
      241 tatctgtgat taatgctgcg acactcttac gtgatacgat cgtacctttg aatggttcat
      301 tgcgtgaggt aagctcataa tcaataatat cttcatcagt cagccaggca gggcgaagaa
      361 ttgtatattc cagtccagaa gcctcaattg catccgcagc cctgcggaat ggttttaggg
      421 gctcaccaat aactgcatta ttccattcgc caaattttcc tggaacttca tcataaatac
      481 cgagggaaag aacaaagatc agacgtttaa catcacaggc tttcatcgca gcgatcactg
      541 aatttgcctg aatatctaaa tcttcccccg taaggttggc atacacaacg tcctgtccct
      601 gcatggcctg cttcagtgct gcatgattca gtacatctcc cataataatc ttgctattag
      661 ttggataggg tttgtggatt tttgctggct gacgggcaaa taaagtttgt ttaatggttt
      721 gtttatctgc cagttgatta atcacatggc gggctatctg accgccagca ccaagaatca
      781 gtacattttt cataatattt ctcatgaaac ttatcccaga tgggtgttga aaaaaggaat
      841 aactttttcc agcgcctcct gaactgcttc cggtttgtca tacagttcat aatgtgagaa
      901 atcgggtaat gaaactaact gacgatcctg cgaaaccatc gcccggccat agacttccag
      961 tccatcacga taagcaccga aggcaccgac tttttcacca actactacca tcaccggctg
     1021 agtcagtaac acttcagtaa atgcgaaggc atcccaggcc agcgttttct gtgcatgtga
     1081 gaacaacatt tttgtcgcac ctcccggttg ttgcccgcgt ggtgttttat agtagtcagt
     1141 tgcttcataa acatcgcgtt cggtcagtcc atgggctttt gctgcatcca ggctggcggg
     1201 aagtaattcg ttgatctgta attctccacc acgagcctct ttggttcgtt gcgacgccat
     1261 tgcattcaa
//
LOCUS       DAZPJP010000401         1268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPJP010000401 DAZPJP010000000
VERSION     DAZPJP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.404"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            237..755
                     /locus_tag="ACHU9J_004549"
     CDS             237..755
                     /locus_tag="ACHU9J_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1907887.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            965..>1268
                     /locus_tag="ACHU9J_004550"
     CDS             965..>1268
                     /locus_tag="ACHU9J_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1907888.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          311 a          312 c          326 g          319 t
ORIGIN      
        1 cggactttga acagttgggt atggttatcc agatatatat tattaagtta ttgctgaata
       61 agagatttaa tgattttaaa aatagttttt acgacaaccg taaattctag ctcggtttgt
      121 atgtaaaaaa tactgaaaaa tgatgcggtt ccgtgaagtg agctgcatct gcctgtctgg
      181 gattaacggc aggtcattgt aagtagtaat taaccacgtt aacaaggagt ttacaaatgc
      241 caacaccgtg ttatatctcc atcactggtc agacccaggg aaatattact gcaggtgcat
      301 ttaccgctga ttctgtcggc aacatctatg tgcaggggca cgaagacgag atgctggtgc
      361 aggaattcct gcataacgtt actgtcccga ccactcctca gtccggtcag cctgcgggtc
      421 agcgtgctca caagcctttc atttttaccg tggcgctgaa caaagcggtt ccgctgatgt
      481 acaacgcact ggcctccggc gaaatgctgc caaccactga acttcactgg tggcgtacct
      541 ctgtggaggg caaacaggag cattatttca ctacccgcct gactgattcg acgattgtcg
      601 atatgaaact gcatatgccg cattgccagg acccggcgaa acgcgagttc acccagttgc
      661 tcgaggtttc actggcctac cgcaaaattg aatgggaaca cgtcaaatct ggcacctccg
      721 gtgccgacga ctggcgcgca ccactggaag cataagttaa gccaacagca tccggctatc
      781 aggccggatg ctgtttttgt attacgccgt agaaagaaag gttgtcagta aggggttacg
      841 ttcacgggag atggcggatg ccggaaactt cggcggtagc gtgcctgaac gcaagcgtaa
      901 cactgtactg tcacagccgc tttaacagcg aactgtctgt ttttccttaa ccccggaggt
      961 tgctatgtca accggattac gtttcacgct ggaagtggac ggcctgccac cggatgcttt
     1021 tgcggtggtc tcctttcatc tgaaccagtc actctcttcg ctcttttccc tcgagctctc
     1081 tctggtcagc cagcagtttc tctcccttga atttccgcag gtactggaca aaatggccta
     1141 cctgacgata tggcagggcg atgacgtaca gcgccgggtg aaaggtgtgg tgacctggtt
     1201 tgaactgggg gagaacgaca aaaaccagat gctgtacagc atgaaggtat gcccaccgct
     1261 gtggcgca
//
LOCUS       DAZPJP010000402         1253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPJP010000402 DAZPJP010000000
VERSION     DAZPJP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.405"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(223..>1253)
                     /locus_tag="ACHU9J_004551"
     CDS             complement(223..>1253)
                     /locus_tag="ACHU9J_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HFR1907889.1"
                     /translation="TIRTHAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCA
                     GAIASPQILQRSGVGNAELLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYP
                     ALQWWNQPKIGAEWLFGGTGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGS
                     NAVKEHGFQCHVGSMRSPSRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITR
                     EIMHQPTLDQYRGREISPGTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDG
                     EGRVHGLEGLRVVDASIMPQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVAN
                     GMPVRAKK"
BASE COUNT          280 a          353 c          313 g          307 t
ORIGIN      
        1 gttcactcat aatatatcca gaaaataaaa tctgccgcag ataaggttgt attaatagtc
       61 tgtatcagga atgttcgggg taaatatcag caaaaaacca gcatcatgaa tactggatat
      121 gaagcatgag agttacctca gtgtttatat aaggattcgg tccccctctc tggaacggta
      181 actctcaatc tgatcggttc ctgcgttagt tcacatcacg actcattttt tcgctctcac
      241 cggcatccca tttgccacaa aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg
      301 aatcatatcc gctattttct cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat
      361 aatctgcggc ataattgacg catccaccac gcgcaggcct tccagcccat gcacgcggcc
      421 ttcgccgtca accacggaca tctcgtcgta acccattttg caggtaccgc acggatggaa
      481 ggcggtttcg gcgtgattac gcacgaactc atcgagctgc tcatccgtct gacattccgt
      541 gccggggctg atttcgcggc cacgatactg atccagcgtc ggttgatgca tgatctcccg
      601 ggtgatgcga attgcgtcgc ggaactcctg ccagtcctgc tcgtgtgaca tgtagttaaa
      661 cagaattgcc ggatgctggt gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg
      721 cgagcgcatt gagccgacgt ggcactggaa accgtgctcc ttcaccgcat tcgagccgtt
      781 atagttaatc gctactggca ggaaatggta ctgaatattc ggccacgcaa attcctctcg
      841 gctgcgaata aatccgcctg cttcaaagtg gttgctggca ccaacgccag tgccgccaaa
      901 cagccactcc gcaccgattt tcggctggtt ccaccactgc agggcagggt agagggaaac
      961 cggttctttg cactcatatt gcagatacat ctccagatga tcctgaagat tttcgccgac
     1021 gccgggtaat tcatgcacca gcggaatatc aaactccgcc agcagttcag cgttgccgac
     1081 gccggagcgt tgcaggatct gcggtgaggc aatcgcgcct gcacataaca gcacttcttt
     1141 gttggccgtt gcgcgggttg ggatggtgct gtcgccttcc agccattcga cgcccaccgc
     1201 gcgtttgccg tcaaaaatga tgtgatcggt catagcgtga gtacgaatgg tca
//
LOCUS       DAZPJP010000403         1237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPJP010000403 DAZPJP010000000
VERSION     DAZPJP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.406"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            131..808
                     /locus_tag="ACHU9J_004552"
     CDS             131..808
                     /locus_tag="ACHU9J_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1907890.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            880..1164
                     /gene="ppnP"
                     /locus_tag="ACHU9J_004553"
     CDS             880..1164
                     /gene="ppnP"
                     /locus_tag="ACHU9J_004553"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1907891.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          309 a          273 c          313 g          342 t
ORIGIN      
        1 gaatctttac attatgccgt gcacgtctgc tgctacgctt tttgtcattt gtagcacaag
       61 taagtgtcag cagtggtgct tcacacttgc ccggtaatta acgacgaaag aaaagtaagg
      121 tggatgaaca atgagtgcgt cgttggcgat cctgaccatc ggcattgtac ctatgcagga
      181 ggttttgccg ctcctgacgg aatacattga cgaagataat atttcccatc atagcctgct
      241 ggggaagtta agtcgtgaag aagtgatggc ggagtacgcg ccagaagcag gcgaagacac
      301 cattctcaca ttattaaatg acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg
      361 agacctgcaa ggtgtggttg aagtgctcga taatcagggt tatgacgtca ttttattaat
      421 gagtacagca aacattagta gtatgactgc gcgtaatacg atctttcttg agccgtcgcg
      481 aatattgcct ccactggttt cctctattgt tgaagatcat caggtggggg ttatcgttcc
      541 ggttgaggag atgctgaccg ttcaggcgca aaaatggcaa attttgcaga aatcgccggt
      601 attttcattg ggtaacccca ttcatgattc agaacaaaaa atcattgatg ccgggaaaga
      661 attactggca aaaggggctg atgtcatcat gctggattgt ttaggatttc accaacgtca
      721 tcgcgattta ctgcaaaaac agctcgatgt tcctgtcttg ctgtctaacg tattgattgc
      781 acggctggct gcggaattac tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta
      841 tagtgaagta gatgttcaac taccagacag ggccagttta tgcttcaaag taatgagtac
      901 ttttccggca aagtgaaatc aatcggcttt tccagcagca gcactggtcg cgccagcgtg
      961 ggtgttatgg ttgaaggcga atacaccttc agcaccgctg agccggaaga gatgacggta
     1021 atcagtggcg cgctgaatgt gttactgcct gacgcgaccg actggcaggt gtatgaagcc
     1081 ggttcggtgt ttaatgttcc cggtcacagt gagtttcatc tgcaagttgc cgaacccacc
     1141 tcttatctgt gccgctatct gtaattcctc gccttcccct tgaacgggag ggcatttttc
     1201 tgaaatatcc tctctttaac ccataataat atttcct
//
LOCUS       DAZPJP010000404         1236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPJP010000404 DAZPJP010000000
VERSION     DAZPJP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.407"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(421..1044)
                     /locus_tag="ACHU9J_004554"
     CDS             complement(421..1044)
                     /locus_tag="ACHU9J_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1907892.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          329 a          262 c          252 g          393 t
ORIGIN      
        1 gcggcactcg atatttttaa attcctgtat caagtactga aaattatttc atcgccactt
       61 ccaattcctt tctgcatggt aagtagtgag gacgaaaaga agaatatgat aaatatttat
      121 tcgtggtaat cagagagagt cgcgacattt gtgatgttat tcttacaact gtaagaagcg
      181 ccggtgattt acaagtaaga gtatgattac gctttttaaa gcgtaccata aagcataaca
      241 taataaatga tttgtgatga cactggatta ttgcggttta ctttgaggaa aataaaatgc
      301 gtgtttatag aaccgcgagc gccaaataat tttatgacca caacctgtcg tattgtggtc
      361 ataaaaaatt gtcggctgga gggagttaaa tcctttacct caatatactg cctgtcagca
      421 ttagttcaca aacagtgctt cgctatcgac gatgattcca tcttccgcac ctttaagcgc
      481 caggtttgcc tcgtagtatt gctttttatc aagcagtttc tgcgcatcag caagtgcatt
      541 acgagtctgt ttaagcggca tcagatattg gttttccatt acgccaacac cggccaggcg
      601 aagctcttcc attgcgccct ttttatcccc tttcgccatt ttttcattgg caatttttat
      661 cgcggcttct ttttcgggtg ttgctacata actctcagaa attccgacag atgcattaat
      721 gacaatatat tggtcatcat tgagattggt ctttttacct ggcttagcga actttgccca
      781 ttcggtactg tcatctgaca gcaaagcgga agcttcattg gtcagttctt ttgccttttc
      841 cggatcgcca tgaaacagag ccaggcgcgc tacctgcacg tcacgcatcg catataaacc
      901 ttgttcagag atacgctctg ctgctgccgt tttctgggtt tgctgagttt gctgttgtgc
      961 cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat
     1021 cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg
     1081 ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt
     1141 tatcttacaa ttgtagttat tatcgcaaag cggcatttag tcttacagtt gtaggaatat
     1201 gcttcctgtc gcagagaact attcttatat accaca
//
LOCUS       DAZPJP010000405         1234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPJP010000405 DAZPJP010000000
VERSION     DAZPJP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.408"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
BASE COUNT          361 a          245 c          209 g          419 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaacatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgcttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgtgtaat gcctggcata gcaagccctt
      601 ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct gtttagaaat gccaaattgt
      661 tgatctaaca aataaacctg tataaacaga acaccattta atctattccc ctgtgtaacc
      721 caccacatca cacaataaaa acaaaaaact ttaattatat ttcatagcat acaaaagtca
      781 atagaagccc tttcgtgctc ctacgtaaaa aatccatcac ttttcccctg atacatcaat
      841 tcaaggcgtt atatgtgttc cttagcacat ataacattta ttatgttaag tggattttgg
      901 cctatatttt gacctgcacc cgcataaaca ctggtttcat cgcttacagc ccttgtgaga
      961 cgtggtgctg tttctagcat ctgatccgac ataaaaccga tcattctcgt gtttagtgaa
     1021 agtttagtga gagacattac agaaaggctt tctgtgtaat gatttaatag gtaaaaacga
     1081 tgttgattac gcaagaaaat tccttgctga tatttgggga ttctggattt tccagatcgg
     1141 ggggcgctga aaatcgcgtt aagcacctct cgcaatctag ccataaggga agggatatag
     1201 gtattaagat gtatttatag ctatttatgg ctat
//
LOCUS       DAZPJP010000406         1230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPJP010000406 DAZPJP010000000
VERSION     DAZPJP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.409"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..1196
                     /gene="bluF"
                     /locus_tag="ACHU9J_004555"
                     /pseudo
     CDS             1..1196
                     /gene="bluF"
                     /locus_tag="ACHU9J_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          351 a          263 c          270 g          346 t
ORIGIN      
        1 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
       61 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      121 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      181 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      241 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      301 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      361 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      421 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      481 cttgattcct gtaccctttc accaactata aacgaccact ttgcctttca tcccttatcg
      541 cggcggataa tcgcttttga agccattgtg caaaaaaatg aagcccatca gccatagcgg
      601 ttgggcagcg taaagacggg gaaatctaca cagcggatct caaaagtaag gcgcttgcat
      661 tcgcgatggc acacgcactt gagctcggtg ataaaatgat ttcaatcaat ctattaccta
      721 tgaccctggt taacgaacct gacgcagtct cttttttact tgatgaaata aaggccaatg
      781 ctctggtgcc tgaacaaatc atcgttgaat ttactgaaag tgaagttata tctcggtttg
      841 atgagtttgc agaagcgatt aaatcgctta aggctgccgg tatcagtgta gcaattgatc
      901 attttggcgc aggttttgcc ggtttgttac tcctgtcacg cttccagcct gacagaatta
      961 aaatcagtca ggaattgatt accaatgttc ataaaagcgg gccacggcag gcaattattc
     1021 aagcgatcat aaaatgctgt acatcacttg aaattcaagt cagtgctatg ggcgtggcaa
     1081 caccagaaga gtggatgtgg cttgaatccg caggaattga gatgtttcag ggagatctgt
     1141 ttgctaaagc taaattgaat ggtatccctt ccatagcgtg gccagagaaa aaataatttt
     1201 cagcacattc tttcacatga tttcagtaaa
//
LOCUS       DAZPJP010000407         1210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPJP010000407 DAZPJP010000000
VERSION     DAZPJP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.410"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHU9J_004556"
     CDS             complement(270..1013)
                     /locus_tag="ACHU9J_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1907893.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          326 a          289 c          282 g          313 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac
//
LOCUS       DAZPJP010000408         1200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPJP010000408 DAZPJP010000000
VERSION     DAZPJP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.411"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            76..759
                     /gene="tssD"
                     /locus_tag="ACHU9J_004557"
                     /pseudo
     CDS             76..759
                     /gene="tssD"
                     /locus_tag="ACHU9J_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            739..>1200
                     /locus_tag="ACHU9J_004558"
     CDS             739..>1200
                     /locus_tag="ACHU9J_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907894.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          376 a          237 c          262 g          325 t
ORIGIN      
        1 aggcgtaaat aaaggtaaaa aaaacaacaa tactatttct ctttcacaaa agaattaaca
       61 aaaggttttt caaggatgag tgatattatt tacctgacca taaccggaga acaacaagga
      121 tgtatttcct ctcgttgtgg aacgagcgca tcaataggta atcgctggca gattggccat
      181 gatgatgaaa tcttcgcgtt ctcgctctct aatagtatta cgaatacagg caaagggtca
      241 taattgcatg gccttagttt ctgtaaattg attgacaaaa gctccccgtt acttattaac
      301 gctattaaca ataacgagca gttgttcatg gagtttgact tctatcggat caataggttt
      361 ggtcgatggg agaaatatta caacattcaa ctccgcggcg cgctcttatc agcaatcaat
      421 cacctgttta ctgagaacaa tctggataca gaagccatca ctgtcagtta ttaatatata
      481 ctctccaggc atctgattgc taatactgaa tttagttatt gggcatttcc cgataactac
      541 aaccgtctgt ttataccacg gccaaaaact aaagctggta atggtttcaa aacacttaat
      601 agtaaagcgg ttggcagact gcttgctgct gtcggcatat acaatgggaa tatcgaagga
      661 ttcagggaaa cagcagacaa gttgggggga gatgcacctg ctggttatga tcaatttatg
      721 gataacaaag ggttactcat cgctggtgct tcaatagttg ctggagcagt tctggggaga
      781 atgagactcc ctgatttgga atcacttgag catttcggag ccagaggagc agtatcagga
      841 agaccattta atcccgagct tgccggtggt ccaatagaaa acctgacgac agatggcgta
      901 acaatcaaca gggaaggtat cgctattgtt gaaaaacata tagccagatt tggtcatgat
      961 cctgtaaatg aagtgatgat caatagactg aaagacattg agaaaggaaa aatcccccca
     1021 gaacaagtag atcttaactt ctatactcat gagtgtcggg aatatcagcg ttactgtaat
     1081 cttggttggg aaacaggaca acctgatggt gacgcaggct atgcactctg gaatcatact
     1141 ggatttgccc ctatatttcc agacatctgt tatcacttaa cccattacaa gcccgctgcc
//
LOCUS       DAZPJP010000409         1196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPJP010000409 DAZPJP010000000
VERSION     DAZPJP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.412"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            348..962
                     /gene="evgA"
                     /locus_tag="ACHU9J_004559"
     CDS             348..962
                     /gene="evgA"
                     /locus_tag="ACHU9J_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1907895.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            967..>1196
                     /locus_tag="ACHU9J_004560"
     CDS             967..>1196
                     /locus_tag="ACHU9J_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFR1907896.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQ"
BASE COUNT          378 a          224 c          246 g          348 t
ORIGIN      
        1 aacacagtgc atgtgttgca cattatccca ggaacgttgg aggcctaatt taaatgaggt
       61 gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa tacaattctt acgcctgtag
      121 gattagtgag aagacttata gtgctaactt gaaactataa atcatcggta taatccctga
      181 ttttattgtt gacatttcat ttatgccgac tatttatatg gtatacttgt cgaattatct
      241 taaaggaagc tcagattttc ttatttttat tgagaaaatg agatgacgcc ttatgtctgt
      301 attactacag ggagaaggga gatgcttcat tgcaaaggga ataatctatg aacgcaataa
      361 ttattgatga ccatcctctt gctatcgcag caattcgtaa tttattgatc aaaaacgata
      421 ttgaaatctt agcggagtta actgaaggcg gaagcgccgt tcagcgggtg gaaacactta
      481 agcctgatat cgtcatcatt gatgtcgata tccccggagt taacggtatc ctggtgttag
      541 aaacgctgag gaagcgccaa tatagcggaa ttattattat cgtctccgct aaaaatgacc
      601 atttttacgg gaaacattgt gctgatgctg gcgctaatgg tttcgtgagt aaaaaagaag
      661 gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa tggctactgc tatttcccct
      721 tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca gcaaaaactc gactccttat
      781 cgaaacaaga aattagtgtc atgcggtata ttcttgatgg caaggataat aatgacattg
      841 cggaaaaaat gttcatcagc aacaaaactg tcagcactta taaaagtcgc ctgatggaaa
      901 aattagaatg taaatcactg atggatcttt acacattcgc acaacgtaac aaaatcggct
      961 aaccacatga agtttttacc ctatattttt cttctctgtt gtggtctttg gtcgaccata
     1021 agtttcgcag acgaagatta catcgaatat cgtggcatca gtagtaacaa ccgtgtcata
     1081 cttgatccag tacgtctgag caacaaggaa ttacgttggt tagcgagcaa aaaaaatctt
     1141 gtgattgcag tacataagtc ccaaacggct acgttgttgc ataccgattc gcagca
//
LOCUS       DAZPJP010000410         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPJP010000410 DAZPJP010000000
VERSION     DAZPJP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.413"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHU9J_004561"
     CDS             complement(<1..755)
                     /locus_tag="ACHU9J_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1907897.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHU9J_004562"
     CDS             1071..>1187
                     /locus_tag="ACHU9J_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907898.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPJP010000411         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPJP010000411 DAZPJP010000000
VERSION     DAZPJP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.414"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..126
                     /locus_tag="ACHU9J_004563"
     CDS             <1..126
                     /locus_tag="ACHU9J_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1907899.1"
                     /translation="SKTKLTKSECEKMLSGEKEAGVSREQKVKLINFNCEKLQSS"
     gene            226..441
                     /gene="xisR"
                     /locus_tag="ACHU9J_004564"
     CDS             226..441
                     /gene="xisR"
                     /locus_tag="ACHU9J_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR1907900.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            443..>1187
                     /locus_tag="ACHU9J_004565"
     CDS             443..>1187
                     /locus_tag="ACHU9J_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1907901.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          412 a          215 c          272 g          288 t
ORIGIN      
        1 tcaaagacaa aactaacgaa atcagaatgc gaaaaaatgc tctcagggga aaaagaagca
       61 ggcgtttcca gagagcagaa agtaaaactg ataaatttta attgcgagaa acttcagtcc
      121 tcgtgaattg cattgtattc aaattaaaac ttcatagctg attattaata atcaacatcg
      181 ggcgtcaatt tcagcctaac attggcgcct gccagaggtg atgcgatggc acaagtaatc
      241 tttaatgaag agtggatggt tgaatacggc ctgatgcttc gcactggtct gggtgccaga
      301 caaattgaag cataccgcca gaactgttgg gtggaaggct tccacttcaa acgagtatct
      361 cctttaggga agccagacag taagcgaggg attatctggt acaactatcc gaagataaat
      421 cagtttatca aagactcatg atatgtctaa attaccaaca ggtgtcgaga ttcgaggtaa
      481 atacattcgc atctggttca tgtttcgagg aaaacgatgt cgggaaacat tgaaaggctg
      541 ggagattact aacagtaaca ttaaaaaggc cgggaattta agagcgttga tagttcatga
      601 aatcagttcc ggtgaatttg agtatttaag acgttttccc cagtccagca ctggggcaaa
      661 aatggtgaca acgagggtca taaaaacatt cggggagctt tgtgatatct ggacaaaaat
      721 taaagagaca gagttaacaa caaacacaat gaagaaaacg aaatcacaat taaaaacact
      781 caggataata atttgtgaga gtaccccgat atcgcatatt cgttatagcg atatcttaaa
      841 ctaccggaat gaactgctgc atggagaaac gctttaccta gataatccaa gatccaacaa
      901 aaaaggaaga accgtgcgca cagttgataa ctatatcgcc ctgctctgtt cgttgttacg
      961 ttttgcgtat cagtcgggat ttatatcaac caaaccattt gaaggagtaa aaaaattaca
     1021 gcgaaacaga ataaagcctg acccgttatc taaaacagaa ttcaatgcat taatggaaag
     1081 tgaaaaagga cagagccaaa acttgtggaa atttgccgtt tactccgggc ttcgtcacgg
     1141 ggaactggca gctctggcgt gggaggatgt ggatttcgag aagggaa
//
LOCUS       DAZPJP010000412         1184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPJP010000412 DAZPJP010000000
VERSION     DAZPJP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.415"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            8..1090
                     /locus_tag="ACHU9J_004566"
     CDS             8..1090
                     /locus_tag="ACHU9J_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1907902.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          329 a          257 c          268 g          330 t
ORIGIN      
        1 gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc
       61 gatgtctgct caggcagctg aaatttataa taaagacagt aacaagctgg atctgtacgg
      121 gaaagttaat gctaagcact acttctcctc taatgatgca gatgatggtg atactactta
      181 tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat caactgactg gtttcggtca
      241 gtgggaatat gaattcaaag gcaaccgcgc tgaatctcaa ggttcctcca aagataaaac
      301 ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc tccatcgatt acggccgtaa
      361 ctacggtgta gcatacgaca tcggtgcgtg gactgacgtc ctgccagaat tcggtggtga
      421 cacttggact caaacagatg tgttcatgac tggtcgcacc actggtgttg caacttatcg
      481 taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt atcagggtaa
      541 aaatgaccgc actgacgtaa ctgaagctaa tggtgatggt ttcggtttct ccactactta
      601 tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa tctgaccgca ctaataatca
      661 ggttatctac ggtaacaaca gcctgaatgc atctggtcaa aatgctgaag tatgggcagc
      721 tggtctgaaa tatgatgcga acaacatcta tctggctacc acctattctg aaacccagaa
      781 catgactgtt tttggtaata accatattgc caacaaagca caaaacttcg aagtagttgc
      841 acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct tacctgcaat ctaaaggaaa
      901 agacttgggt gcgtggggtg atcaggacct ggttgaatat attgatgtag gtgcaaccta
      961 ttacttcaac aaaaatatgt ccacttttgt tgattacaaa atcaacctga ttgataagag
     1021 cgatttcacg aaagcatctg gcgttgctac cgatgatatc gttgctgtag gtatggttta
     1081 ccagttctaa tttgattact aaaagatatg ttgcgggagg ctttgcctcc ccaacatata
     1141 agtggctccc tcaagccact tcctttagga gcacaacctt gctt
//
LOCUS       DAZPJP010000413         1169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPJP010000413 DAZPJP010000000
VERSION     DAZPJP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.416"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..230
                     /locus_tag="ACHU9J_004567"
     CDS             <1..230
                     /locus_tag="ACHU9J_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907903.1"
                     /translation="YEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAVLIFWHELAIN
                     GYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            230..820
                     /locus_tag="ACHU9J_004568"
     CDS             230..820
                     /locus_tag="ACHU9J_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907904.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(838..>1169)
                     /locus_tag="ACHU9J_004569"
     CDS             complement(838..>1169)
                     /locus_tag="ACHU9J_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1907905.1"
                     /translation="KARAIALMDSKKMWRGNYAPPLLRILWRLGIRLPPLPFMPFWQV
                     TLLMGSLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLMASFHWWRRKVNR
                     LPSWDDV"
BASE COUNT          266 a          342 c          351 g          210 t
ORIGIN      
        1 gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat ctggacagcc
       61 agggcgcgat ttatctctgc gagtcagcca aagccggagc cgtgctgatc ttctggcatg
      121 aactggccat aaatggctat gcctcaatga atgccataaa gagacaggag ctcattgacg
      181 ccgactttca gcgcctcaga aacctcatct ggccggagga tgacaggtaa tgacccagag
      241 ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc ttgatgaaaa
      301 acggccggga cccgtgctga cgcgggacct ggaacgggtg ctggaacgaa gcggggaggc
      361 cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg ccggctggct
      421 tcgtaccctg cgcgccccga atctgcagct ggctgtcgag ctgacggacg ccggccgggc
      481 cgtggcacag cctctgctgc tggcagaaca ggaccgcatc cgggcagaac agcgtgcggc
      541 agaagtcgtc gtactgccgc tggtcccggc cgcaggtctg cccgcagacg gcacttctgc
      601 cactgatctg gcggtggagc tgaacggcat gacgtatcag gcctgccgtg gggattttgt
      661 ggtacgtctc gacggcagca cctgtctgca gttatggaat aaagaaggca gggtggtacg
      721 cctggagggc gatccgctgg aagtggcgca gtggctgcag gcctgtcacg atgccggaat
      781 ggaggttcgt gtacaggtta atgaaagcgt caccccgtaa aaacatacca gcacggttta
      841 cacgtcatcc catgatggca gccggttaac tttcctgcgc caccagtgga aagaggccat
      901 gagcagtccg aacaaaaaac cacccgtgat acttataata atggcctcac ctgccaccat
      961 tcctgacggc ccccagtaga taaaccacat tgcacatccc caggaaatgc cccataagct
     1021 ccccatcaac agcgtgacct gccagaacgg cataaagggt aatggcggaa gccggatacc
     1081 cagccgccag agaatacgca gaagaggagg ggcataattg ccccgccaca tctttttgct
     1141 gtccatcagg gcaatggccc gggcttttt
//
LOCUS       DAZPJP010000414         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPJP010000414 DAZPJP010000000
VERSION     DAZPJP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.417"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHU9J_004570"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHU9J_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR1907906.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHU9J_004571"
     CDS             complement(510..>1162)
                     /locus_tag="ACHU9J_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1907907.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZPJP010000415         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPJP010000415 DAZPJP010000000
VERSION     DAZPJP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.418"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHU9J_004572"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHU9J_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHU9J_004573"
     CDS             572..>1157
                     /locus_tag="ACHU9J_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1907908.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZPJP010000416         1151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPJP010000416 DAZPJP010000000
VERSION     DAZPJP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.419"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            53..436
                     /gene="traM"
                     /locus_tag="ACHU9J_004574"
     CDS             53..436
                     /gene="traM"
                     /locus_tag="ACHU9J_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR1907909.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFSQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            623..>1151
                     /gene="traJ"
                     /locus_tag="ACHU9J_004575"
     CDS             623..>1151
                     /gene="traJ"
                     /locus_tag="ACHU9J_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFR1907910.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISIQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIA"
BASE COUNT          377 a          180 c          235 g          359 t
ORIGIN      
        1 ttttagtgtt tcgaaattaa ctttatttta tgttcaaaaa aggtaatctc taatggctaa
       61 ggtgaacctg tatatcagca atgatgccta tgaaaaaata aatgcgatta ttgagaagcg
      121 tcgacaggaa ggggcaaggg aaaaagatgt cagtttttca gcaacagctt caatgcttct
      181 tgaactgggg cttcgtgtac atgaggctca gatggagcgt aaagagtctg catttagtca
      241 gactgagttt aataaattgc ttcttgaatg cgttgtaaaa acacaatcat cagtagcgaa
      301 aattttgggt attgagtctc tcagtcctca tgtctccgga aatccaaagt ttgaatatgc
      361 caatatggtt gaagatatca gggagaaggt atcatctgag atggaacgat tttttccaaa
      421 aaatgatgat gaataaaaga aatttgactt cgttcaaata tcagagtttt tatgatttaa
      481 aaaggtgaca gtacgaaaga caattagtat attaattacg tggttaatgc cacgttaaaa
      541 tttgaatttg aaaatcgccg atgcagggag ttctctcctc cctgcatcga ctgtccatag
      601 aatcctttgt gaggaggttc ctatgtatcc gatggatcgt attcaacaaa aacatgctcg
      661 tcaaatagat ctgctggaaa atctgacggc agttattcag gattatccaa atccagcctg
      721 tatcagggac gaaactggaa aatttatttt ttgcaatacg ctgtttcatg agtcatttct
      781 tacacaagat caaagtgctg aaaaatggct tctgtcgcag agagattttt gtgaattgat
      841 ctctgtcaca gagatggaag catacaggaa tgagcatacg catcttaatc ttgtagagga
      901 tgtttttatt cagaacagat tctggacaat atctattcag tcatttctta atggacacag
      961 aaatatcatt ctgtggcaat tttatgatgc tgctcatgtt cgtcataaag acagttataa
     1021 tcaaaaaacg attgtcagtg atgatatcag aaatataatc agaagaatga gtgatgattc
     1081 ttctgtatca tcatatgtaa atgatgtctt ttacttatat agcaccggaa tcagtcataa
     1141 tgctatagca a
//
LOCUS       DAZPJP010000417         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPJP010000417 DAZPJP010000000
VERSION     DAZPJP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.420"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHU9J_004576"
     CDS             <1..122
                     /locus_tag="ACHU9J_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907911.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHU9J_004577"
     CDS             119..376
                     /locus_tag="ACHU9J_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907912.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHU9J_004578"
     CDS             376..750
                     /locus_tag="ACHU9J_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1907913.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHU9J_004579"
     CDS             747..>1148
                     /locus_tag="ACHU9J_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1907914.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZPJP010000418         1142 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPJP010000418 DAZPJP010000000
VERSION     DAZPJP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.421"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHU9J_004580"
     CDS             <1..184
                     /locus_tag="ACHU9J_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1907915.1"
                     /translation="EYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNY
                     RIALRKYQHYQRQTCT"
     gene            317..1117
                     /gene="tcyJ"
                     /locus_tag="ACHU9J_004581"
     CDS             317..1117
                     /gene="tcyJ"
                     /locus_tag="ACHU9J_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1907916.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          247 c          304 g          273 t
ORIGIN      
        1 tgaatatgtc gttcgtctgc gccgtttggg taatcatcta cacgagcaaa atatttccct
       61 cgatcagctg caggacggtt tccttgatga aatcctcgcc ccgtggctgc ccaccaccag
      121 caccaacaat taccgcattg cgttacggaa gtatcaacac tatcagcgtc aaacgtgtac
      181 ctgacttgtg cagaaatcct ccagtctgcc tgcttctgat atatattaaa taagaataag
      241 atgtagcgga gttgtttttg tgtttacaaa caatggctct acactgcaaa cagacataac
      301 aacattcggg gtgaatatga aattagcaca tctgggacgt caggcattga tgggtgtgat
      361 ggccgtggcg ctggttgcgg gtatgagcgt taaaagtttt gcagatgaag gtctgcttaa
      421 taaagtcaaa gagcgcggca cgctgctggt agggctggaa ggaacttatc cgccgttcag
      481 ttttcagggg gatgacggca aattaaccgg ttttgaagtg gaatttgccc aacagctggc
      541 aaaacatctt ggcgttgagg cgtcactaaa accgaccaaa tgggacggta tgctggcgtc
      601 gctggactct aaacgcattg atgtggtgat taaccaggtc accatttctg acgagcgcaa
      661 gaaaaaatac gatttttcta ccccttacac catttccggt attcaggcgc tggtgaaaaa
      721 aggtaacgaa ggcaccatta aaacagccga tgatctgaaa ggcaaaaaag tgggtgtcgg
      781 tctgggcacc aactatgaag agtggctgcg gcagaatgtt cagggtgtcg atgtgcgtac
      841 ctatgatgat gacccgacca aatatcagga tctgcgcgta ggccgtatcg atgcgatcct
      901 cgttgaccgt ctggcggcgc tggatctggt gaagaaaacc aacgatacgc tggcagtaac
      961 cggtgaagca ttttcccgtc aggagtctgg cgtggcgctg cgtaaaggaa atgaagatct
     1021 gctgaaagca gtgaatgatg caattgcgga aatgcaaaaa gatggcactc tgcaagccct
     1081 ttccgaaaaa tggtttggtg ctgatgtgac caaataatca gcataatgac aaaaaagggc
     1141 gc
//
LOCUS       DAZPJP010000419         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPJP010000419 DAZPJP010000000
VERSION     DAZPJP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.422"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHU9J_004582"
     CDS             <1..>1140
                     /locus_tag="ACHU9J_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1907917.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPJP010000420         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPJP010000420 DAZPJP010000000
VERSION     DAZPJP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.423"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            22..579
                     /locus_tag="ACHU9J_004583"
     CDS             22..579
                     /locus_tag="ACHU9J_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907918.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            580..858
                     /locus_tag="ACHU9J_004584"
     CDS             580..858
                     /locus_tag="ACHU9J_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907919.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            861..1133
                     /locus_tag="ACHU9J_004585"
     CDS             861..1133
                     /locus_tag="ACHU9J_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1907920.1"
                     /translation="MKKWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
BASE COUNT          391 a          213 c          228 g          308 t
ORIGIN      
        1 ttatgagatg tcagaacatg aatgcagtat tcagaattta gagcgatgct tcaggcatca
       61 agagaacgca acaagcacaa ctcctacgct tacacaaaca accccaccag ctacgaagtc
      121 ccaaccttta gcaaatccga acgtaagaac attgaagcgg tcattcgtag cattacgcca
      181 cgagacagat ttatgccagt acgcaaaaca acaaagaata ctattaaaaa ttatctggct
      241 aactttgaca gttacgagca gttaccaagc aagcttgatg atgtttttat tgggttctgt
      301 agaagtgaag gccacccgaa atacaacaaa aaactatttt atctgctaaa gaatcttgac
      361 gaaattaacg catccaccgt gacaaatcat cttcagcgac aggcaaccag acttagttac
      421 gaattaccaa gcgataaata ttgcgctttg cttgctgtga tgtgcgcgaa attaattggc
      481 attgttgaac atcatattgc cgttgggaat attgagccaa tggaaaacga gcaaccagat
      541 ttcgagtttg acccctattt agttacagag gatttttaaa tgaataaaca cacattaacc
      601 agagttacaa agacagcctt tgatgaactg aaccgaatca aaaacattac aggtctgcca
      661 cactcagcag tgatgaagct tgcaattaac cgtgatatta acgaacagga tttgcttaat
      721 agtcgcttag aggctgcaag acaccatatc aggattaagc aagatgacct tgatagtctg
      781 aaatccttaa atgcttttgg actggattta gcaaggttac taagtatcaa aattcacaaa
      841 ctgtctcagg aggtctgatt atgaagaaat ggcgagatga tattaaacgg ttctttgcaa
      901 cttacttcat gattaattat gagacaggaa tgcttgaatg gattgatggt ggagaggtta
      961 agacaagaga tgatgcttac ggtaatccaa tgattcgtta tggcactcgt gattattatc
     1021 tgaagtatgt catctggttt ttgcatcatg aagttcaggc aactaaaaag attatcttca
     1081 gggatggtaa taaaagaaat tgccacccaa acaatttact cctggagatt taatgttatg
//
LOCUS       DAZPJP010000421         1132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPJP010000421 DAZPJP010000000
VERSION     DAZPJP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.424"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            5..295
                     /locus_tag="ACHU9J_004586"
     CDS             5..295
                     /locus_tag="ACHU9J_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907921.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            295..714
                     /locus_tag="ACHU9J_004587"
     CDS             295..714
                     /locus_tag="ACHU9J_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907922.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            882..>1132
                     /locus_tag="ACHU9J_004588"
     CDS             882..>1132
                     /locus_tag="ACHU9J_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907923.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPE"
BASE COUNT          364 a          237 c          236 g          295 t
ORIGIN      
        1 tttaatgaga cacacagtta cacgcctttt gccagaggca tattccagac tggcagcaat
       61 caaagacaaa tacccagcca tgagccacac agcacttgtt ggtgagattc tgaaggaaca
      121 cacagccctt acaccgtttg acgctatcaa gggcgttgca gagggttcta aggccatcag
      181 aatcacaacg ggccaactgg aactactcaa agcacagtcc gcagtggcag gttgctcaat
      241 gattcaggtg ttgagtgcca aggttcatca actggcggat aagctggggg tttaatgcta
      301 tgcacatttg attatcatgc gttttatcgc catagaggtc accttgtccg tgagaataac
      361 ggaacatgga aagatttaac tcgtaatgct gaacccttca cacaccagca attaaaatcc
      421 cgcttctcca gatggaagaa tgcaccagca gatactggtg attttgagat tatccatacc
      481 ttcgattatg cctcagaaga tgaccttcac gatgcaatta agctgattga agacttcaat
      541 acctcagaag atgtggatgg tgacaaatat aaatcccgtg gtaaccgcta taaccgtgat
      601 gatatcactc aggccataaa tgctggtcaa tctcagcgat acattacaga aaaatatggt
      661 atttcacgga gacagtattt ctacgttaag tcattgatta ataaagacaa ttaaaaataa
      721 ccatctaaga aacggtagaa tgcactttac aggcagagtt gcaccttaat ttgataaatt
      781 accttaccag ccagtgcaac tccctctgta acccgcatgg ttactggcct cagaatggct
      841 attttaagag gtctgaggtg tgtttcatag gagatttaaa aatgaataat tacccaatgc
      901 aaattatggt tgataacgac actgctatga tggtgcaaag cttcattgat gcaggtatcg
      961 caattaattt tgataaatta ctaaagctga tggctgataa tgcagaaaac atctctgact
     1021 ttattcagtc ggtggagttt aacgagcctc gaatgatgct accaattaca gacagcaaca
     1081 tgaaaagatt ggttatcgag caaacgaaca tatacagtat ttccccagag ca
//
LOCUS       DAZPJP010000422         1130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPJP010000422 DAZPJP010000000
VERSION     DAZPJP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.425"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
BASE COUNT          326 a          247 c          217 g          340 t
ORIGIN      
        1 aatgtcaagt acttttaaca ttttcgtaac atctgtggta taatgggttt atggggagag
       61 taaaatatga ggcgtggcta tgtatttggt cgcatatttt gcattgcaca cagacctctt
      121 caatcgccct gtaacgagtt aaacgacctc ttcaggtcaa ctcataaggt caggtacttt
      181 ttgtgtcaga atgcgattga gagcgtctta caaggaaaac taatggtaaa tctccctcct
      241 gagctggggt cacctgatgt atggactggt ggaggtattg ggctgattgc aggcctgcta
      301 cttcgctacg tcctcgtcag agtgcttgtt gtctttaaac tgttcggtcg tacatggacg
      361 cttgacctta aggataacga ccacacagac ccccgttaaa ctccttcaaa acccctgcac
      421 gagatgacag atagaagtct atggtacagg ggtgtcttta gcattttgtg ctatctatta
      481 aaaaatagct tgacagaacc ggaaagctgt atctatcaag cattcacctc tctgacagta
      541 atttctaaaa gttcttattt ttcaataagt tgtaattagc catatataga gaactgacaa
      601 gtctcttcac agacaactct gcaaccttaa cagtaacttc taaaaatgct tatttttcaa
      661 cgagttgtaa tcacccaata tataaggctg tccagtacct tcacagataa cttcaagccc
      721 tcttaaccgc cccactaatc gggagaagag cgataacccc agagagggaa gctcctttct
      781 taaccgacct cttaaacgac ctttacagac cttgttcaag gtaatcacaa tagtttttct
      841 tccctcggtt tcctgtgttc ctcggctggc tattgagatt tgaaagacct ctgtaagcgc
      901 tctcttagcg agagaaaaat cattcatagt gaccccaagc gaagcgcccc tctaaaaaaa
      961 gattttttag ctattttgtt taaaagcata tcaaacgata cattttattg atttaggtca
     1021 aagacaaaag cattgacaaa atggtaagat tgttcacatg accttactta tttaagagac
     1081 tttgaaacag cctgtttaac aggtcactac ctctggtctt atcgttccct
//
LOCUS       DAZPJP010000423         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPJP010000423 DAZPJP010000000
VERSION     DAZPJP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.426"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHU9J_004589"
     CDS             84..1115
                     /locus_tag="ACHU9J_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1907924.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPJP010000424         1110 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPJP010000424 DAZPJP010000000
VERSION     DAZPJP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.427"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHU9J_004590"
     CDS             complement(<1..478)
                     /gene="umuC"
                     /locus_tag="ACHU9J_004590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1907925.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(478..897)
                     /locus_tag="ACHU9J_004591"
     CDS             complement(478..897)
                     /locus_tag="ACHU9J_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1907926.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          311 a          291 c          235 g          273 t
ORIGIN      
        1 tctgccgctg ccattttttt gccgcatgat tggcaagctt agccagcgtt ttggtctggg
       61 cgatccccat accaacagta agatgggtac gttgtagcac cgttgcgcga atttctctgc
      121 caaaatcagt cagatcgcga caattacgca cacctgtcag atcgcagaat gcctcatcaa
      181 tactgtaaat ctcgacgcgg ggcgatagtt cttccagcgt cgacatcacc cgattgctca
      241 tgtctgcgta aagctcataa ttgctgctaa agcaaaccac accacagcga cgaaacagat
      301 ctttttgttt gaaccaggga tcgcccattt taacacccag cgcctttgcc tcagcgtttc
      361 gggcgataac gcaaccgtca ttattcgata gcacaaccac cggtttaccc cataaatcag
      421 ggcgaaacac cgtctcacag ctggcataaa acgcgtttac atcacagaga gcaaacatca
      481 gcgcatcgcc ttaacgacgt ggatcaccac accaaagaca tccagcgtat cttcgctact
      541 gatggtaatg ggcgagtacg cactgttcat gggaataagc tgtaccgtcg ggcgtagttg
      601 caattttttc accgtaaact cgccgtcaac agcagcgatg acaatatcac catggctggc
      661 ggtaatagcg ctatcgacaa tcagtaaatc accgtcacta attccaccat caatcataga
      721 atcaccactt gctttgacga agtaagtcgc gctgggatgc tggatcaaca gttgattcag
      781 atcgatgcgc tgttcgacgt aatctgctgc cggtgaagga aagccacact gaacaagatc
      841 gctaaatagc ggaaaagtca caatttcgcg gagatccgca ggcttgataa acaacataat
      901 aatctgcctg aagttatact gtttttatat acagtaatct gttcttgcca gcagatcaat
      961 actgattcag gctatcaata tttgtcgctg catagactgc tgatttttcg ttctcttatc
     1021 ctgtgctcac gtggccttct ggcgacgacg ctcatccagc agaaatgaaa aatatcaccc
     1081 ggcaaaaaaa tagaatagaa gcattcgcca
//
LOCUS       DAZPJP010000425         1105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPJP010000425 DAZPJP010000000
VERSION     DAZPJP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.428"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..504
                     /locus_tag="ACHU9J_004592"
     CDS             <1..504
                     /locus_tag="ACHU9J_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1907927.1"
                     /translation="SAGDTYNLIKTLDVCHYDSETKVLTSHHGIIRNEENECYCLGAL
                     QVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNNTTAFR
                     PKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPG
                     LSCFQPQ"
     gene            766..>1105
                     /locus_tag="ACHU9J_004593"
     CDS             766..>1105
                     /locus_tag="ACHU9J_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907928.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTIN"
BASE COUNT          390 a          178 c          207 g          330 t
ORIGIN      
        1 tctgccggtg atacgtataa cttaattaaa acgctcgatg tttgtcatta tgactcagaa
       61 acgaaagttt taaccagtca tcacggaata ataagaaatg aagaaaatga atgttattgt
      121 ctcggtgctt tgcaggttcc atttaatcag atgaaagacc caattgatcc tgaggaatta
      181 gcaaatatat ttaataaaaa acataaacaa cacatggatg atcgtcttat ccatctaatt
      241 agaagcaatg ctgtagcatc cacacctgta tatgaaaatt attttaataa cactactgct
      301 ttcagaccaa aacctgaaga tatcttcaaa tgtggacaga cgctcaaaaa aactaaacag
      361 aaatatggcc attatggatt aggggtagat cagcatcaaa aaattgataa ttataccatg
      421 gggctaaatt cctggaagat agcacctaac gaacgtggtg ataaaaaagg tgtcccaggg
      481 cttagttgtt ttcaacctca ataaaacttc atgagtgctg tggtatctct ttttaatttt
      541 gccagtattt cataggtttt cttaatcgat tgtagaggca tgttctgatg acgcctctac
      601 aatctttagg gactttctct gctatgagat aagaaatttc atattatgct ttaaggctga
      661 atgatttgtg caaaatataa ttattttccc atcgaacatg aggtgtatgt cataagatta
      721 aatgttaact atgtattcct cgcggtaaat aatatgaggt aaaagatgaa aattggaact
      781 gtggcaggca ctaacgacag tacaacgaca atcacaacaa atgatatggt gcaagagcat
      841 gttaccaact tcactaaaga actgtttggg tatattgcaa atggtatagg tgatgatata
      901 tcaagtattg ccaggacaat gctcggtgag gttgtggaaa aaatagatga ttggcaaata
      961 gaacggttcc aacaatctat tcaagatgac aaaatttcat ttaccataca aacgaatcat
     1021 tcagaaaaat atagtatgct atcgggcatg cgcgctcata ttctacgtag gaataactgt
     1081 tatcagttta ttgtgacaat taata
//
LOCUS       DAZPJP010000426         1091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPJP010000426 DAZPJP010000000
VERSION     DAZPJP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.429"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..594)
                     /locus_tag="ACHU9J_004594"
     CDS             complement(<1..594)
                     /locus_tag="ACHU9J_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907929.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT          335 a          238 c          179 g          339 t
ORIGIN      
        1 cagcttagcc agtcttgatt tcagcaaatt aatctcattt tctcttgctt tcatcagttt
       61 ctctttggca atttcttcaa gctcagcttg tgatttatca gcaacaatta gagacagtcg
      121 atgtggtgct ctgacagctc tttctgtacg aacaactctt tgaccttctg gcaaatctgc
      181 aatcacttta ccaatctcta cagggtcata cccccaaccg tcacggtcta gtcgagagaa
      241 acaagcataa gaattggttt gaagctccat cgaaataacc atttcgtcaa tttcgtcttc
      301 aagcttggac tgaattttct cgtaaacact tctcattgct tcttgagagt ctttaatagc
      361 ttggaaatat cggcgttgta gcttcaactt caaatcaagt tcacaccgtt cgttcataga
      421 aaaatcaatt tcaactcttg gaatctcgag tggttctggt ggaatatatg gacctttatt
      481 tacttcgtgt gattcccgcc aagctctatc aaaagattca cgcaaagtta attcgtgaat
      541 atcaagtgca cttggtctgt gttcgataag catttcatgt tcatttctaa tcattcgttc
      601 accccttagt ttatttgtta tttcaattta tagaagcaca ctcagcaatc agaggtgaag
      661 agtctgactg aacaagaacc tgccaagtgt gcttttaaaa actgaaaaag ccctaaacat
      721 ttctgtgaag ggcaaaggat tcaataatga atgaacaaga gtactttttg agatagcctc
      781 tcagactaac ctgaaaggcc atgacgaaaa gcattgcacc tttccaactc agaatatcca
      841 tccatcggat tattgaaaga aaaataagag agaattgctc tcccataatt aaaattatgc
      901 catgcttaag gttatttgtc aacagctttt taacaggttg ataacatcaa ttcttattta
      961 ccgcattgtt aataatgcaa ccaacaaata agccaagcaa cgtccaagca attattaaac
     1021 ccattagtga accctcacag aatccgtcac cagaatatta tcactgtaca ggattgtcac
     1081 agcacctttc a
//
LOCUS       DAZPJP010000427         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPJP010000427 DAZPJP010000000
VERSION     DAZPJP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.430"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHU9J_004595"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHU9J_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHU9J_004596"
     CDS             294..>1087
                     /locus_tag="ACHU9J_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1907930.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPJP010000428         1086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPJP010000428 DAZPJP010000000
VERSION     DAZPJP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.431"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(506..>1086)
                     /locus_tag="ACHU9J_004597"
     CDS             complement(506..>1086)
                     /locus_tag="ACHU9J_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1907931.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          343 a          207 c          190 g          346 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacggt
//
LOCUS       DAZPJP010000429         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPJP010000429 DAZPJP010000000
VERSION     DAZPJP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.432"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHU9J_004598"
     CDS             complement(<1..643)
                     /locus_tag="ACHU9J_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1907932.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHU9J_004599"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHU9J_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1907933.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZPJP010000430         1072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPJP010000430 DAZPJP010000000
VERSION     DAZPJP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.433"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..1072)
                     /locus_tag="ACHU9J_004600"
                     /pseudo
     CDS             complement(1..1072)
                     /locus_tag="ACHU9J_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          164 a          416 c          296 g          196 t
ORIGIN      
        1 cccttctcat catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc
       61 tgccgctggc tgttatagcc ataccgtacc gtcctgccgt cctgccgtcc ggcagtatca
      121 ctgaagtcag ctttccagac gccatatgca gtcggtactc cgtccgccgt cccgccgcat
      181 ccgtctgcgc cttcagcctc cccgcctcat catactcgct gcgggtgatg ctcccgtcgg
      241 catgctcctt cttcaccacc cgcttcaggc caccctcgcc ttccgtgtac agcacctccc
      301 gccggttcag gctgtccgtg atgattaccc ggctctctcc gtactcaaag cggtagtcca
      361 gcccctccgg gttgacctgc tccgtcaccc gaccggtatc atcataccgg tagcagctct
      421 ccggcctccc cgcataatgg tgcgccacca tccgcccggc gtgctccgca tcataagtaa
      481 acccgcgcac ctgcatcccg ctgcggtcat acaccgcccg cagttcacca ccggccgtgt
      541 acgtgtagcg cgccagcggc gcagtcggct gttcatccgg gtatgccggg tcgtgtgtca
      601 gccataccgc ctccagccgg ataccgttat cggcaccgta ctctgtaccg gcgggcagcg
      661 tgtcggggaa ggaaggagct gagagagggc agggactgtc aggggaagat aacgaagcgg
      721 tgtgcggttt acgggcctct tccgcccgct gcgcctgtgt ggtcagcacc aggtgaaaac
      781 ggcggcccgc gccgtccatc acccccgtca ccgcgcccgc cacgtcaccc tccgctgccc
      841 ggtgaaaggc cagcgtccgc ccgaagccgt ccaccacgcc cgtcagcacc cggtatgcgg
      901 gcggctccgg cggcagcacc tcgtccgccc ccggcacccg ctccggccag ctgagtatcc
      961 accacggccc ctgaaggctg tttgtcgcca ggtacacatg cggactcagg cgaacatctt
     1021 ccggcagcac ctgccagagc gcgcttagcg gctgcgaact gtgctgcgcc gc
//
LOCUS       DAZPJP010000431         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPJP010000431 DAZPJP010000000
VERSION     DAZPJP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.434"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHU9J_004601"
     CDS             <1..120
                     /locus_tag="ACHU9J_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1907934.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHU9J_004602"
     CDS             120..410
                     /locus_tag="ACHU9J_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1907935.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHU9J_004603"
     CDS             407..961
                     /locus_tag="ACHU9J_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1907936.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAZPJP010000432         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPJP010000432 DAZPJP010000000
VERSION     DAZPJP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.435"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHU9J_004604"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHU9J_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHU9J_004605"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHU9J_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHU9J_004606"
     CDS             728..985
                     /locus_tag="ACHU9J_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1907937.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccct
//
LOCUS       DAZPJP010000433         1037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPJP010000433 DAZPJP010000000
VERSION     DAZPJP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.436"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHU9J_004607"
     CDS             complement(<1..906)
                     /locus_tag="ACHU9J_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR1907938.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          293 a          236 c          207 g          301 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagt
//
LOCUS       DAZPJP010000434         1037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPJP010000434 DAZPJP010000000
VERSION     DAZPJP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.437"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(197..772)
                     /locus_tag="ACHU9J_004608"
     CDS             complement(197..772)
                     /locus_tag="ACHU9J_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1907939.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          283 a          226 c          216 g          312 t
ORIGIN      
        1 tgaaatcaat aaagattctt gtgcagtctg aatagataac ttagcgttat gtctagtcgt
       61 ttccctctcc attcctaaaa agtgctgtat actggcctat acaacgctat aaagacctct
      121 acatagcgcc ttgatagcta tgtcacaggt acaaaaaagc cccctataaa ggaggctgtg
      181 tagtctatta agggtcttac agggtcttgg tccagtgtct gctaacagtc cttacagaga
      241 ggttaagaac cttagctacc tgagactgag atagtcccct ttccttgtac tctaagaccg
      301 ctctagttgt gtcactcgct ataggtcttc caagtacctt accttgaact ctagcacgct
      361 ctagaccctg tttagtgcgc tctctgatac ggtttctttc aaactctgca aaggtactaa
      421 atagctgtag aaccaactta ccctctgagc ttgaaaggtc tttaacgggt aaatctaaac
      481 agatgagctt cacaccctta tcattaagaa gggtcactgt attctgtagg tcgatgttat
      541 cccttcccag acggtcaagt ttaagaacaa caagggtgtc accttcttct agcttatggt
      601 tgaccagatt cgcaaatccg tccctatcca tagctgacac tgaaccagag acaacctcct
      661 ctataactcg tgacttagga atatcatagc cagccttctt aatggcttga atctggttct
      721 ctgtggtgga agtctctact gatacacggc agtaagcaaa ggttcgtgac atagatatcc
      781 ctctgggtag tcattaaacg gttagtttgt cttgtctata gacattaaag caaatggtag
      841 gtttgttgtc tatagctata gtgactcttc tatgtagata ttaaacggac gtttcttgtc
      901 gtgggtcaca tagatacttt tagggcttct gtcaagtgat attcgtagat tttgcaaggg
      961 ggaggtgtaa ggctgaaaca gggctactaa gaacacatac atctaaggct ggaaagtacc
     1021 tgagcagata ttcttca
//
LOCUS       DAZPJP010000435         1031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPJP010000435 DAZPJP010000000
VERSION     DAZPJP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.438"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>1031)
                     /locus_tag="ACHU9J_004609"
     CDS             complement(<1..>1031)
                     /locus_tag="ACHU9J_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR1907940.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDRQG"
BASE COUNT          172 a          384 c          284 g          191 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg c
//
LOCUS       DAZPJP010000436         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPJP010000436 DAZPJP010000000
VERSION     DAZPJP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.439"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHU9J_004610"
     CDS             complement(77..865)
                     /locus_tag="ACHU9J_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1907941.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHU9J_004611"
     CDS             complement(905..>1023)
                     /locus_tag="ACHU9J_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1907942.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPJP010000437         1020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPJP010000437 DAZPJP010000000
VERSION     DAZPJP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.440"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHU9J_004612"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHU9J_004612"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1907943.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          287 a          239 c          223 g          271 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccaa tagttcatcc agtgcttcaa tatcgcgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggtagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      601 cgactaatga cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcgttaata
      961 attaaaggga gtaacgtatt atgtcagaag aaaataaaga aaatggattt aatcatgtca
//
LOCUS       DAZPJP010000438         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPJP010000438 DAZPJP010000000
VERSION     DAZPJP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.441"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            289..>1009
                     /locus_tag="ACHU9J_004613"
     CDS             289..>1009
                     /locus_tag="ACHU9J_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907944.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGANFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLN"
BASE COUNT          390 a          191 c          148 g          280 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaata
//
LOCUS       DAZPJP010000439          978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPJP010000439 DAZPJP010000000
VERSION     DAZPJP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.442"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..823
                     /locus_tag="ACHU9J_004614"
     CDS             <1..823
                     /locus_tag="ACHU9J_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1907945.1"
                     /translation="VLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLN
                     GFKFQDLPLSRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSE
                     LGSTNLYTQVIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVR
                     DFLNDYMSENRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLA
                     VGAALALREVPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
BASE COUNT          323 a          175 c          188 g          292 t
ORIGIN      
        1 tgttctctcc gaagatcccg atttagatgg gcgaattgag attgttgatg gctcacaacg
       61 agttaggact atacacgctt ttatccaaaa tgaattagtt ttacaagacc tgaaatcttt
      121 agacgtcctt aatggtttca aattccaaga tttaccatta tcacgtcaac gtcgtttcat
      181 gagaattcct gtccgcgtta tagaattgag ttctaaatgc aatgaagaaa cccgtagaga
      241 tctttttgag cgtattaact ctggcagcga tatattgaaa gatatggaag tccgtaaagg
      301 atcagaactt ggttcaacaa atttatatac acaggttatt aagccatgct caacaatacc
      361 taaatttaaa gaattagcac ctttatctca gactaaagaa aaacgagatg aacggttaga
      421 atttacatta agattctttg cctacgtaga taagtacaag aactttgatc attccgtacg
      481 ggattttcta aatgactata tgtcagaaaa tcgagatata gacgaaacta aacaaaactc
      541 aatgaaacaa gagtttgcta atgttctatc ttttgttgaa aactatttcc cagcaggatt
      601 caagaaaact attactgcaa aatctacacc tcgagttaga ttcgaatctc tcgctgttgg
      661 cgcagcactt gcattacgtg aagtccctga ccttgttccc aagaacttag attggctaag
      721 ctcagaagaa ttcaaacgat tgaccacttc cgacggagct aatagtcgcg tgaaggtaat
      781 tgaacgaatt gaatatgttc gggataaatt attagaggat taacatggat cgttttcgtg
      841 aggattttga cgaaaggtca ggagagatct tggcctattt ggatcttttg aaatttattg
      901 agtatgccgg tgcagaactg atatcagctg atgataaaga acataagttc tccattactg
      961 ctcaatcacg aaaaactc
//
LOCUS       DAZPJP010000440          975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPJP010000440 DAZPJP010000000
VERSION     DAZPJP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.443"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHU9J_004615"
     CDS             complement(<1..502)
                     /locus_tag="ACHU9J_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1907946.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKR"
BASE COUNT          252 a          239 c          237 g          247 t
ORIGIN      
        1 ggcgtttttc gcctttggcg tcagtgtagg tgacgtcctc aaaattgagg gcgttaaatt
       61 caggtgagca ttcagtattt gcgatggcac gtaacacatt gtcgtgacgc ttgccaaaga
      121 actctgcgat cgcaatagac gtagtgacag cgcgaccatt ttcgatggtt acgtcagggt
      181 gagaaagggc agggatagta gccatgatgg catcctcgag tgataagtta attaactcac
      241 caccgaggtt ttccacgacc ataagggtgg tgagacgtac aggggtggaa ataccggtca
      301 ctcgagaacc cggccagtct tgcgactgcc ctgcacatcc caccataatt tgaatggagc
      361 tgtgcattac gcataaaaaa accgcttcag cgcggctatg cgctcgagta acgttcgggt
      421 ttccacgccc ggcacccgtt ttataaggtg caggtgcact ataattccac ccgttctggt
      481 tttcaatagc tacattcaac attttctctt tcctttcatc accgaagtga actttgttga
      541 tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc ggatacaggg
      601 aaacccataa ccacccgata cgggggaact aatcgctttt taactgtgcc gcgtgcgctg
      661 agccgcattc accgcatcac aaaattcact ttaaaaaggg cggacatcag ccagcaatga
      721 aactgatgcc gccaaaggta ccaatcaaca tggagtgttg tggcgtggtt gtcacttaag
      781 cgtatggtca acctgacaac ccggtgtcct caacggggaa ggaataactc cgccatactt
      841 accgccgcgt catttcgcgt tgtgtgcctg cttttaacca cgtcaggcga ggtggtatcc
      901 ttcttatccc gaataaccaa gaaggaaatc tatatgacta aagaagaatt tgtctcttat
      961 atttttgata aaacg
//
LOCUS       DAZPJP010000441          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPJP010000441 DAZPJP010000000
VERSION     DAZPJP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.444"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>974)
                     /locus_tag="ACHU9J_004616"
     CDS             complement(<1..>974)
                     /locus_tag="ACHU9J_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1907947.1"
                     /translation="SHYKVSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKI
                     KFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILI
                     LKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALLLIVMDSYINKRINYKLY
                     CFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPI
                     GQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAI
                     LIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCA"
BASE COUNT          343 a          164 c          178 g          289 t
ORIGIN      
        1 aagcacaaag caatattatg gtgacaaaca cagcagtcgg aaccggttta gaaaagagta
       61 ttacatcact taggcctact accagtagtg aaaaaagcaa gattaaaata taaggttctt
      121 tagcagttct taaggcatat atgagtattg ctgaaaaggc taaaattgtt aatacaactc
      181 caggaatacc acgcgttgat aacgtatcta tgagatcgtt atgcaaatga gaatctatat
      241 agggtaaagc gccactcaat ctaggctctt tttcttctag ttcatgtatt ttttccgctc
      301 gtttttccag tgattgtcct attggagaat atgtttttaa gccaacttca tacattgcca
      361 gacgggcacc gactgaagtt ctggtgttat catgcgaata attaactaaa tcgttattta
      421 agtcattcat tctcataagc aatgtatctt taaaagaaaa tacacctgct aataatgcaa
      481 tcgcaataaa acaatataac ttataattaa ttcgcttatt aatataacta tccattacga
      541 ttaatagtaa cgcaaagaat ggaaacacta atatggctgc acgcgtttca gttactatga
      601 cagcacataa gctaagcatg aagttaattg tatataatgt tgttttatgt ctaaagtcgg
      661 attttaaaat gagaattgat gccagtaaag aagggaaaag gatgatgtag cctgctgttg
      721 tagcgtggga agctgataat gcaacacggt caacgtttaa atagaaatgt tgccacatgg
      781 catgcgcaaa aaaaagtaac tgcgttgcca gggatgcgaa cagaattagt ttcccaaatt
      841 ttatttttgt tctcaactca tttttagcaa aaagaaccaa gaaaataaaa gcactacaca
      901 aggcaatttt cccagtttcc attgggccac gatatgcatt ggtataaaca gagcctgaaa
      961 ctttataatg tgaa
//
LOCUS       DAZPJP010000442          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPJP010000442 DAZPJP010000000
VERSION     DAZPJP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.445"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..950
                     /gene="tssI"
                     /locus_tag="ACHU9J_004617"
     CDS             <1..950
                     /gene="tssI"
                     /locus_tag="ACHU9J_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1907948.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNN"
BASE COUNT          250 a          260 c          298 g          166 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaactag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPJP010000443          972 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPJP010000443 DAZPJP010000000
VERSION     DAZPJP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.446"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            45..830
                     /locus_tag="ACHU9J_004618"
     CDS             45..830
                     /locus_tag="ACHU9J_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907949.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          321 a          177 c          215 g          259 t
ORIGIN      
        1 gctaaacatt accatgatta tgacaggcga gggagtgtcc gattatgaat ttaagaaggg
       61 tttgttgtat atgtgtatta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg
      121 atgaaaagaa gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc
      181 aactgacaga caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata
      241 ttcaagctgc gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg
      301 ttcaatccgg caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata
      361 ctgtttccac attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca
      421 aagataaaaa tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac
      481 tgcgttttgt ggctgcaaaa gggcatcaga aagcgattat tgttttccag gatacgttgc
      541 agacaggaaa atatgactct ggattgaaat caatagtttg gtcagattat aaaaatcaaa
      601 aattgactga cacgatgtca ctgcgctatc tggtacgatt cacgctggtg gatgtagcta
      661 caggtgagtg ggctgcctgg tcgccggtga attatgaata taaagtgctt ccaccattgc
      721 ccgacaagaa tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc
      781 aaaagaccta taaagcgatg gtaaaagatt tggttaaccg ctatcaataa taaattatat
      841 ctgccgccag gaatttctgg cggaaataat acaaaattct ttgcataaga aatttttctt
      901 gacaaagaca gtgcaagaaa ggacatggaa tccggagtta aaaactgttt gttttagaat
      961 gtttaattat tt
//
LOCUS       DAZPJP010000444          971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPJP010000444 DAZPJP010000000
VERSION     DAZPJP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.447"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHU9J_004619"
     CDS             complement(<1..228)
                     /locus_tag="ACHU9J_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR1907950.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHU9J_004620"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHU9J_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>971)
                     /locus_tag="ACHU9J_004621"
     CDS             complement(586..>971)
                     /locus_tag="ACHU9J_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907951.1"
                     /translation="FKSSIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALS
                     FILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQL
                     NVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          318 a          166 c          188 g          299 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac a
//
LOCUS       DAZPJP010000445          962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPJP010000445 DAZPJP010000000
VERSION     DAZPJP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.448"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>962
                     /gene="htrE"
                     /locus_tag="ACHU9J_004622"
     CDS             <1..>962
                     /gene="htrE"
                     /locus_tag="ACHU9J_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR1907952.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          251 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 at
//
LOCUS       DAZPJP010000446          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPJP010000446 DAZPJP010000000
VERSION     DAZPJP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.449"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHU9J_004623"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHU9J_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZPJP010000447          953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPJP010000447 DAZPJP010000000
VERSION     DAZPJP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.450"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(29..829)
                     /locus_tag="ACHU9J_004624"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="ACHU9J_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          273 a          221 c          180 g          279 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aat
//
LOCUS       DAZPJP010000448          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPJP010000448 DAZPJP010000000
VERSION     DAZPJP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.451"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(198..>936)
                     /locus_tag="ACHU9J_004625"
     CDS             complement(198..>936)
                     /locus_tag="ACHU9J_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1907953.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          259 t
ORIGIN      
        1 attgcttctc cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac
       61 ttgttgaccg agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg
      121 attatcctgt ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt
      181 ttactgctgg catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata
      241 cctaatataa ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat
      301 atcacttccg tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga
      361 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac
      421 agcggaatac cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga
      481 gcggcaatcc acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac
      541 aaaagatatg gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg
      601 ggattaatac ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct
      661 ttagccgctg gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt
      721 tttgctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
      781 cccagagaat ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
      841 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      901 aatggtgaaa caaataaaaa agtgagagtc ggacca
//
LOCUS       DAZPJP010000449          926 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPJP010000449 DAZPJP010000000
VERSION     DAZPJP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.452"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            71..382
                     /locus_tag="ACHU9J_004626"
     CDS             71..382
                     /locus_tag="ACHU9J_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907954.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            515..>926
                     /locus_tag="ACHU9J_004627"
     CDS             515..>926
                     /locus_tag="ACHU9J_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907955.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASL"
BASE COUNT          290 a          151 c          206 g          279 t
ORIGIN      
        1 gtgctggatt tttgggaaac tgactgcaat actgtacata tatacagtaa tacatagagg
       61 agggtaacaa atgagcaaag acggctatga tccatccatt acgcgagggg ttcagaagtt
      121 ctcacataac ggtcgctggg caaccgtgtg ttattgggga tctgcactaa cagaatttca
      181 ttatggggat agggtattct tccagaatca gtacagggaa tactggttag gtactataga
      241 gagagattgt tttgtactta cctgtgaaac accactaata agtgttattg atgggttagc
      301 atatctatct gctgagtacc ggatgaatca aagcagtgaa gaaggtgatt ggttctgtga
      361 tcagggggaa ctacccttct gaggatacac gtactgctca ccccaacaaa ataacaaacc
      421 caacatgtga gcagtacgtg aacgggtagt gtacttgatt gtgtttcgga tggtaaagta
      481 tttgcccctt taatgcgttc aggaactact ttcgatggat tgtagaaaat taaatatcgt
      541 tcttttatcg accgtagcag tattactggt tgttgtaatt ttagtactaa taaaatcact
      601 ctttgttggt tctgaaggtt tcgagtgggg atcggttagt gactgggtta gttctattgc
      661 caatctcatt atggctggag cagccctata tgcagcatgg aacgctaaaa attggtttca
      721 aactaaaata aaggaaaatg ctttaaatca agttactaag ttctggacta attgcgatct
      781 tttttcgttg gagatgttga aaacatatga aaatatatgc aaccttaaca atactgaacc
      841 agatatagaa ccaaaaaatt tcgcacattt agttaaaact gaacgagaag ttatcgatct
      901 attaaacatt aagttagcct cattaa
//
LOCUS       DAZPJP010000450          921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPJP010000450 DAZPJP010000000
VERSION     DAZPJP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.453"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHU9J_004628"
     CDS             complement(<1..264)
                     /locus_tag="ACHU9J_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1907956.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
     gene            321..>921
                     /locus_tag="ACHU9J_004629"
     CDS             321..>921
                     /locus_tag="ACHU9J_004629"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1907957.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSE"
BASE COUNT          263 a          208 c          187 g          263 t
ORIGIN      
        1 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
       61 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
      121 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aattcctgga tgtagaactg
      181 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
      241 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
      301 ttcacaaata aaactataac atggtgaaat taatgaaaaa aaacacagat gatggggcta
      361 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
      421 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
      481 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
      541 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
      601 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcccgcgg
      661 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
      721 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
      781 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
      841 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
      901 taacacatat actctcagag c
//
LOCUS       DAZPJP010000451          918 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPJP010000451 DAZPJP010000000
VERSION     DAZPJP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.454"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..918
                     /locus_tag="ACHU9J_004630"
                     /pseudo
     CDS             1..918
                     /locus_tag="ACHU9J_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          298 a          167 c          181 g          272 t
ORIGIN      
        1 tttaaggaat aacaacaaca cacagaatgc acagtgggct gattatgtgg gtgacattct
       61 tcggggggct cagccgataa accagctagt acctcagcac ccttatctta acgacgtccc
      121 actcattgat gagttaaggc atcagaacac tcatcatgtt attctcttga cacttgatgt
      181 tgcaaaaaaa aaaaaaaaaa aaaatactca gcccaataac atcatttgat tacattcatt
      241 ttatcactac acatccttca ggtataaaag atacactcgc ctggttagtt aacgcgagta
      301 aattaatgac tgagtttgat gacaatggga aaattatatt taacattaat gccttaaagt
      361 atacaaaagc aagttacttt gaaatacttg gagagaaata tataaaaatc acaacatcca
      421 gcccctggct tttagaaaag ctcggtaaat atattttctc ttcacgagcg cctcaagttc
      481 tggaactcgc tattggctgg cgaggagctt tatctgaatc aataaaagga gttaagttct
      541 gtatctggtt ttcagtagcc tggagaacca tagaatttat tatgagttca gagcgagatc
      601 tggttaattt tcttggcgat ttttcaatgg atgtggccaa agcagttata gccggtggtg
      661 ttgcaaccgc tattggaagc ctggcttctt ttgcctgtgt tagctttggc tttccagtaa
      721 ttcttgtcgg aggagcaatt ttactgacag ggatagtgtg tacagttgtt ttaaatgaaa
      781 tcgatgctca atgccattta tcagaaaaat taaaatatgc aattagagat ggactaaaac
      841 ggcaacagga acttgataaa tggaaaaggg aaaacatgac tccatttatg tatgttctta
      901 acactccacc cgtgatat
//
LOCUS       DAZPJP010000452          905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPJP010000452 DAZPJP010000000
VERSION     DAZPJP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.455"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..338
                     /locus_tag="ACHU9J_004631"
     CDS             <1..338
                     /locus_tag="ACHU9J_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907958.1"
                     /translation="FNSNNMTIYWNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCL
                     LDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQ
                     TMKITGTPE"
     gene            350..799
                     /locus_tag="ACHU9J_004632"
     CDS             350..799
                     /locus_tag="ACHU9J_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR1907959.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          304 a          104 c          227 g          270 t
ORIGIN      
        1 gatttaattc gaataatatg acgatatatt ggaatagtag agcatcttta ttttgttcaa
       61 ctgaattaaa ctcgaaatca caatcgcctg cattaggttt gggacatgag tttgctcatg
      121 cgcagtattg ccttcttgat aaggaaaatt tcatggctct tttaagccga acagataaga
      181 aatatgagaa taaagaagag gcaagggtta ttacaataat tgaatcgcgt gcggcgaaaa
      241 ctttaggtga gtgtaccaga ggagctcata gtggactgcc tttttatcgg gtggatggac
      301 cgttgcagac gatgaaaata acgggtacgc cagaatagtg aggtttttta tgatgaaata
      361 tttgatggtt ttattgtctc tattttctgg ttcggtattg gggatggggc gagtgaatga
      421 attgtgtgga atagatagcg tgaaaacgat agagataatt aatcttccat cgtatgttac
      481 cacgttagtt ccgcttagca aggaaggtct taatgaaata tacagatata aagtggtggt
      541 gaatgaaatt agagatcttt atgctggtaa gataattgat ttattgcaaa tgaagtattt
      601 cagaaaagaa aaatataaca atataagatg ggggggtagt atcatttcga aaggtaataa
      661 taaatgtgaa atttattttg atgcttttgg tgaatgtgga agtgtgaatg gcattaacgt
      721 gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa gaaatccctt tgctgtcgca
      781 aaaaataggt gggctttaaa tggtagggca atattcaaag acagtatagg taactatttg
      841 tatggccatg taaggtaaaa gaaaaggaat aaaccatgag cggaaaaccg gcggcgcggc
      901 agggc
//
LOCUS       DAZPJP010000453          905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPJP010000453 DAZPJP010000000
VERSION     DAZPJP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.456"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..351
                     /locus_tag="ACHU9J_004633"
     CDS             <1..351
                     /locus_tag="ACHU9J_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907960.1"
                     /translation="SMNESDLEVSSKKTSKQDREITDVSVDSDHKNTQCPATDDELDP
                     FDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSPKKKQTQDDKKQ
                     KIKPKMYFAGGGIK"
     gene            351..641
                     /locus_tag="ACHU9J_004634"
     CDS             351..641
                     /locus_tag="ACHU9J_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907961.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
BASE COUNT          328 a          157 c          187 g          233 t
ORIGIN      
        1 tctatgaatg aatctgacct tgaagtaagc tctaaaaaga cttctaaaca agacagagaa
       61 atcactgatg tgagtgttga tagtgaccat aaaaacacac agtgccccgc cactgatgat
      121 gagttggacc ccttcgatga tgaagaggat gaacgtgaaa aagccattaa gagggcagag
      181 aagaaaaaga gattcgagct tgcaagacaa aagaacaaag tatcgggttt agacccattc
      241 gatgacagtg aagatgatta ttatctttca ccgaaaaaga aacaaactca ggatgacaag
      301 aagcaaaaaa ttaaaccgaa gatgtatttt gccggtggag gaattaagta atgaaagaat
      361 atcacggtga aaagagatac aaagattacc tgttaaagcg ttacagcatt tctcgtgaag
      421 gtcatcttat gaaggatact cacggggaag tttaccggat tagaccaaag aaagaaggga
      481 ggggttattt cttttacgat ggtgtgacag atttaaagat tgacgctttg aggtttgcag
      541 tgctgtatca ttttgatatc tggaattcaa tccaccagct acgattaaaa gacggtgacc
      601 ccagcaactt aaaggccacc aatattatca caaaacgtta gtttgtttta ccatcatcaa
      661 tcgtaattgt gatttcatat ttgaaaagct taatgctgaa attgaatcgt gtacttctgg
      721 cgataatcat ttttaaaagc tgatacacaa gaagcaccag aaacacaaag caacctaagc
      781 ttgcgaatct gtgagattca gcaattgttt caagaaagcc cattaattcc ccttatttat
      841 caaagggaat gacctgtatc agccccctac aaccatatta acatgtaaat gagttgttga
      901 caata
//
LOCUS       DAZPJP010000454          889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPJP010000454 DAZPJP010000000
VERSION     DAZPJP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.457"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..569)
                     /locus_tag="ACHU9J_004635"
     CDS             complement(<1..569)
                     /locus_tag="ACHU9J_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1907962.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(569..>889)
                     /locus_tag="ACHU9J_004636"
     CDS             complement(569..>889)
                     /locus_tag="ACHU9J_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1907963.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          210 c          194 g          188 t
ORIGIN      
        1 gcaagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc tacagaaacg
       61 ccgccagctg tcagggaaaa cacaattaac acgtttgagg gcggtatcag taatccagta
      121 atacaagatg agacgtttac agcggtagaa aatgccggat cataaccttg tttggtctga
      181 atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc agaaatggaa
      241 ccaaacatca tattcgccag gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc
      301 attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat cagcgttccg
      361 gcaaagatga aaaaggggat tgccaatagg gcaaagttat ccagtccatt agccagtcgc
      421 tgcgaaacag tgattgccgc gatatcccag ggaaacatca aaagcattga agctacagtg
      481 gcaataccga ttgagaacga aatgggcaca ccaataaaga ccagcacaaa aaaacagcca
      541 aacagtgtaa tcgcgatata actatccatt aactggcctc ctgcgcaggt tgtttcagtt
      601 gttgaatgct ttgattaata aaacacaagg cataaaacat catcaccaat ccacttaatg
      661 gcagaatgat atatacgtat cccatcggta tttgcatcgc ggcagatacc tgtgatgttg
      721 ccagcgtttt atcaattaat ttcaggccac cagtcacaat gaccgaacca gcaaaaccta
      781 aaataagaac gttaatgact atactaagaa gtaattgctt gcgcttattc agggcgagag
      841 caaataaatc gatagagaga tgtctctgcg cgccgacggt atatgcagc
//
LOCUS       DAZPJP010000455          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPJP010000455 DAZPJP010000000
VERSION     DAZPJP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.458"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHU9J_004637"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHU9J_004637"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1907964.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZPJP010000456          855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPJP010000456 DAZPJP010000000
VERSION     DAZPJP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.459"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>855
                     /locus_tag="ACHU9J_004638"
     CDS             <1..>855
                     /locus_tag="ACHU9J_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1907965.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYI
                     SSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINV
                     LTNDKVFKA"
BASE COUNT          281 a          156 c          202 g          216 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactggat gcgtgactgc cattctacag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 aaaaaatttc cgctgacaga attaaataat ccagcgcatg acagttccgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtggcagt ctcctttcgc
      601 tcaataatag cagagcaaaa gaaagagccc gaaatgatgg tcagatatta tatcagttct
      661 gctgatttaa ccgctgagaa gtttgcgaca gcgattcgaa atcactggca cgtggagaat
      721 aatctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aatgcagcag aattattttc agggatacgg cacattgcta ttaatgtttt gacgaatgat
      841 aaggtattca aggca
//
LOCUS       DAZPJP010000457          842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPJP010000457 DAZPJP010000000
VERSION     DAZPJP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.460"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHU9J_004639"
     tRNA            54..130
                     /locus_tag="ACHU9J_004639"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHU9J_004640"
     tRNA            173..248
                     /locus_tag="ACHU9J_004640"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="ACHU9J_004641"
     rRNA            432..>842
                     /locus_tag="ACHU9J_004641"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAZPJP010000458          839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPJP010000458 DAZPJP010000000
VERSION     DAZPJP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.461"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..263)
                     /locus_tag="ACHU9J_004642"
     CDS             complement(<1..263)
                     /locus_tag="ACHU9J_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR1907966.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAG"
     gene            complement(280..>839)
                     /locus_tag="ACHU9J_004643"
     CDS             complement(280..>839)
                     /locus_tag="ACHU9J_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1907967.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          241 a          180 c          146 g          272 t
ORIGIN      
        1 ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc cttgttatca
       61 ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc aaaatagctt
      121 agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca taatattgct
      181 ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgtact cgcagaaata
      241 gagaaaaaaa tacaattgtt aataactttg ccatattttt tagtccgtaa ctgttaatgt
      301 gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag atgaaccagt
      361 tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccgaaagcgt ttaaacaact
      421 ttgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta ctaaacgtat
      481 accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc ataccccttg
      541 aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac attgcatatt
      601 attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt tatcatcatt
      661 ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac aggtcgctaa
      721 tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat tccatccccc
      781 cttcagtcca atcttttcgc gccggcgtgt acaccgggtc catctggttt tgcatctgc
//
LOCUS       DAZPJP010000459          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPJP010000459 DAZPJP010000000
VERSION     DAZPJP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.462"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHU9J_004644"
     CDS             complement(246..>815)
                     /locus_tag="ACHU9J_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1907968.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZPJP010000460          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPJP010000460 DAZPJP010000000
VERSION     DAZPJP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.463"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHU9J_004645"
     CDS             complement(<1..494)
                     /locus_tag="ACHU9J_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907969.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          265 a          169 c          145 g          224 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
      661 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aataagatat
      721 gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
      781 tgtaattagc ccagaacttt agc
//
LOCUS       DAZPJP010000461          802 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPJP010000461 DAZPJP010000000
VERSION     DAZPJP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.464"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            47..>802
                     /gene="gspA"
                     /locus_tag="ACHU9J_004646"
     CDS             47..>802
                     /gene="gspA"
                     /locus_tag="ACHU9J_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1907970.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          217 a          181 c          199 g          205 t
ORIGIN      
        1 ttatttttat gattaaggat aatcaataat taaaacagga agttctatgt ctacgagaag
       61 agaagttatt ctttcctggt tgcgtgagaa acgtcagacc tggcgtctgt gctatttgtt
      121 gggtgaggct ggaagtggaa aaacctggct ggcgcagcaa ctgcaaaaag ataaacatcg
      181 ccgtgtgatt actttaagcc tcgttgtttc ctggcaaggt aaggccgcat ggatcgttac
      241 cgacgataac gcggctgaac agggctgccg tgacagcgcc tggacgcgag atgagatggc
      301 ggggcaatta ctgcatgcgc ttcatcgaac tgatagtcgc tgccctctta ttattattga
      361 aaatgctcac ctgaatcacc gccgcatact tgatgatctc caacgcgcga tatctcttat
      421 tcctgacggt caatttttgc tgatcggcag acccgatcga aaagtcgaac gtgattttaa
      481 aaaacagggc attgaacttg tctcgacagg acgtctgacg gagcacgaac ttaaggcaag
      541 catccttgaa ggtcagaata ttgaccaacc cgatctcctt ttaacctcca gagttctgaa
      601 acggatagct ttattatgtc ggggcgatcg cagaaagctg gcgcttgccg gcgaaacaat
      661 tcgcttatta cagcaagccg agcagaccag cgttttcact gcaaaacaat ggcgaatgat
      721 ttaccgcata ttgggtgata accgcccccg gaaaatgcag cttgccgtgg taatgagtgg
      781 aaccattatc gctattacct gt
//
LOCUS       DAZPJP010000462          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPJP010000462 DAZPJP010000000
VERSION     DAZPJP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.465"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHU9J_004647"
     CDS             complement(<1..741)
                     /locus_tag="ACHU9J_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR1907971.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZPJP010000463          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPJP010000463 DAZPJP010000000
VERSION     DAZPJP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.466"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..789
                     /locus_tag="ACHU9J_004648"
                     /pseudo
     CDS             1..789
                     /locus_tag="ACHU9J_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          304 a          137 c          149 g          199 t
ORIGIN      
        1 gaccatatta ctattggtat cgacaaccaa gaaccaatag aattacctgc gaactataaa
       61 gacaaagaac tcgtccgtac tatcattaat gacaacatag ttgagaagac tcatgatatc
      121 aataacaagg aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt
      181 atcttcgata agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg
      241 aatagcgaac caacggactt atttacctgg tacggtaaag ataaaaaagg caactctctc
      301 gctattgtaa ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag
      361 gacgactacc acattcaaag aggaattcgt attaatggtg atagtctcac ccaatattgt
      421 agtgaaaaag ccaggaatgc ttcagcaatg cttcagcgtg gtttgaaagc agtaaagcta
      481 tcatggcaga atcatttgca acaggttccg atcatcaggt tgtaaacgag ctcaacgggg
      541 aaagactgag agaacctaac gaagttttta aacgtttagg tcgagcaata agatataatt
      601 ttcaagtgga cgatgcaaaa tttagacgcg acaatgtaaa agaaataatt tctaatttat
      661 tcgctaacaa agttgacgtt gaccatcctg aaaataaata caaagacttt aaggatctcg
      721 aagataacgt tgaaaaaaga cttcaaaatc gccaggcgaa atatcaaaat gaaattaatc
      781 aactatctg
//
LOCUS       DAZPJP010000464          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPJP010000464 DAZPJP010000000
VERSION     DAZPJP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.467"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHU9J_004649"
     CDS             complement(<1..458)
                     /locus_tag="ACHU9J_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1907972.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHU9J_004650"
     CDS             complement(530..742)
                     /locus_tag="ACHU9J_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907973.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZPJP010000465          774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPJP010000465 DAZPJP010000000
VERSION     DAZPJP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.468"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            44..760
                     /locus_tag="ACHU9J_004651"
     CDS             44..760
                     /locus_tag="ACHU9J_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1907974.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          256 a          134 c          167 g          217 t
ORIGIN      
        1 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
       61 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      121 cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat taaacacatc
      181 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      241 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac gttatgaagg
      301 ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg ttaaccgtac
      361 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      421 tgtcagctat atgtttgaca atgctggtgt gattgtattt aaaggatcag accctgacca
      481 tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg aagaagaagg
      541 taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg ctctaaaagc
      601 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
      661 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
      721 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatctttta aaga
//
LOCUS       DAZPJP010000466          774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPJP010000466 DAZPJP010000000
VERSION     DAZPJP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.469"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHU9J_004652"
     CDS             <1..343
                     /locus_tag="ACHU9J_004652"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1907975.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>774)
                     /locus_tag="ACHU9J_004653"
     CDS             complement(405..>774)
                     /locus_tag="ACHU9J_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR1907976.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          209 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctga
//
LOCUS       DAZPJP010000467          773 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPJP010000467 DAZPJP010000000
VERSION     DAZPJP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.470"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            148..732
                     /locus_tag="ACHU9J_004654"
     CDS             148..732
                     /locus_tag="ACHU9J_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1907977.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          228 a          154 c          160 g          231 t
ORIGIN      
        1 taggcgtcct aatgaacaat ggttgtaaat tttggtaaca aatatcattg ctcattaata
       61 tctagcatct gcttataaca tttgtgtcat gaatagtcat tcttcaaacc agtagtctga
      121 ctaatggaat tttattcaag gatttttatg tctaaaaaat taggttttgc cctgtctggt
      181 cttatgctgg caatggttgc aggtactgct tctgctgata tggatggtgg tcagttaaat
      241 atcagtggtc tggttgttga taacacctgt gaaacccgcg ttgacggcgg taataaagat
      301 ggtctgatcc tgctgcaaac tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac
      361 accgttggcg ctaaagccaa acctttcagc atcaccgttg actgcagcaa agcaaatccg
      421 aatccaggta gtacagctaa aatgaccttc ggttctgttt tctttggtaa tagcaaagga
      481 acgctgaaca acgacatgtc tattaacaac ccgtctgacg gcgtgaatat cgctctgcac
      541 aatattgatg gttccactat caaacaggtt caaattaaca accctggcga tgtgtatact
      601 aaagccctgg atgcaacgac aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt
      661 gccgtcgcag accaaacagc aactgctggt tatgtaaaaa ctaacactgc atacaccatt
      721 acttatcagt aagtattcac caattaaatt gattgaatga atatacaggg aat
//
LOCUS       DAZPJP010000468          771 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPJP010000468 DAZPJP010000000
VERSION     DAZPJP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.471"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..372)
                     /locus_tag="ACHU9J_004655"
     CDS             complement(<1..372)
                     /locus_tag="ACHU9J_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907978.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGP"
     gene            complement(323..>771)
                     /locus_tag="ACHU9J_004656"
     CDS             complement(323..>771)
                     /locus_tag="ACHU9J_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1907979.1"
                     /translation="YRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYR
                     IVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPT
                     GTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGAMPGLFIRTS"
BASE COUNT          169 a          211 c          191 g          200 t
ORIGIN      
        1 gggaccagca ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc
       61 tgcgcgccat gcttcaattt cctgacgcct ggcctctctg gccgcttcca gcatttctgg
      121 tgtcacagtg aatggggcta tctcacccca tttgccactt tgcagttcct gccagatttg
      181 ctgacccgtc ggtgcgacat catcagcggt ggctgtgtag gggactgcct ggtccctgtc
      241 gtcaaaaaaa acgtcacagt ctactgcgcc actttcggta taacggggat taatgatttt
      301 tttaatttcc acggtgcatt cctcacgatg tgcgaataaa aagcccgggc attgcgccag
      361 agacatgtgc atccggcacc ccggacaggg cgcaatatga ccccggtaat gaatgctctg
      421 aacatcccgt aatgaaaaat tgtggggatg ctatatacgt tccggtggga gtactgggca
      481 ctgaaatccc caccggtccc agtcgtgagc ctctgtatga ctgccccctg acaagtctga
      541 tgactttatc accgtcagct tctccctggt acgcagcaat aatcagcccg ccaatgtcag
      601 ggtctcccca tctgttgcgg acagagctcg ccacgattct gtaaataata tcttctgtgg
      661 ttatatttat tttcacccag ccagtcagtc tggatatggg ccagtagcag tagcgggtgt
      721 gataaatggg gccgttaatg ccgtaaaaag taagggattt ggctctgtac c
//
LOCUS       DAZPJP010000469          765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPJP010000469 DAZPJP010000000
VERSION     DAZPJP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.472"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(81..>765)
                     /locus_tag="ACHU9J_004657"
     CDS             complement(81..>765)
                     /locus_tag="ACHU9J_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1907980.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDYRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQLR"
BASE COUNT          177 a          193 c          188 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatagt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAZPJP010000470          760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPJP010000470 DAZPJP010000000
VERSION     DAZPJP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.473"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>760)
                     /locus_tag="ACHU9J_004658"
     CDS             complement(<1..>760)
                     /locus_tag="ACHU9J_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1907981.1"
                     /translation="ERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHP
                     LLYRHTIGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDA
                     VTNNIALMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHY
                     QDLLTRFSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPV
                     PVATMVREDASNLVNLKTLLNDGFFIKYSHFNDVA"
BASE COUNT          248 a          145 c          114 g          253 t
ORIGIN      
        1 gccacgtcat taaaatgact atatttaatg aaaaaaccat cattgagtag tgttttaaga
       61 ttgactaaat tacttgcatc ttcacgtacc atcgtggcaa ctggaacagg cagagacatg
      121 aaaacttctt cattcaccat caagtgaatc atgcattgcg ttccctttgt catatccaca
      181 catacgctac aaatatcgtg gtaatgcgaa gagcgcattt caggaaacag attaaacacg
      241 gtatcttgaa aggcactaaa tcgtgttaat aaatcctgat aatgaacagg ttttgtttca
      301 aaattatcat cagcaaatcc aagcactatt gtatgctcta catctaacgc agtttcaaat
      361 ggctcttctt ctgtaaaatc attatttaaa acctcacctg acgtattatc actgtcataa
      421 aaagtgttca tcaatgcgat attatttgtt acagcgtctt tttcgatatt ctcactaatt
      481 gcatatcgcc tgctataatc ataaaaatga ataaacgcgc cctttataaa ggatatatat
      541 tgttctttgt taaagtcaat acggctgtat aatttttcaa aaatcacatt cgcaatttta
      601 ctaccaatgg tatgcctgta taataatggg tgcgattcaa tcgtaaaatg cttgtctaat
      661 tgatcttgta atttattctc atagtccaga gggaaaagaa actgttgtct ttcaagtttc
      721 ccattcgata cattacgtat tccaatagag attctttcat
//
LOCUS       DAZPJP010000471          758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPJP010000471 DAZPJP010000000
VERSION     DAZPJP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.474"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            85..>758
                     /locus_tag="ACHU9J_004659"
     CDS             85..>758
                     /locus_tag="ACHU9J_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1907982.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYA"
BASE COUNT          252 a          131 c          160 g          215 t
ORIGIN      
        1 aattgagata ttccttatta cctaaaacta tttttcatag cttatatatg atcaaatact
       61 ccttgcataa ataaggagaa caaaatggaa cttaaaaaac tgatggaaca tatttctatt
      121 atccccgatt acagacaagc ctggaaagta gaacataaat tgtcagatat tctactgttg
      181 actatttgtg ccgtcatttc tggtgcagag ggttgggaag atatagagga ttttggtgaa
      241 acacatctcg attttttgaa gcaatatggt gattttgaaa atggtattcc tgttcacgat
      301 actattgcca gagttgtatc ctgtatcagt cctgcaaaat ttcacgagtg ctttattaac
      361 tggatgcgtg actgccattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg
      421 ctccggcact cttatgacaa gagtcgccgc aagggagcga ttcatgtcat tagtgcgttc
      481 tcaacaatgc acagtctggt tctcgggcag attaagacgg atgagaaatc caatgagatt
      541 acagccattc ctgaacttct taacatgctg gatattaaag gaaaaatcat cacaactgat
      601 gcgatgggat gccagaaaga tattgcagag aagatacaaa aacagggcgg tgattattta
      661 ttcgcggtaa aaggaaacca ggggcggctt aataaagcct ttgaggaaaa atttccgctg
      721 aaagaattaa ataatccaga gcatgacagt tacgcaat
//
LOCUS       DAZPJP010000472          757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPJP010000472 DAZPJP010000000
VERSION     DAZPJP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.475"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>757)
                     /locus_tag="ACHU9J_004660"
     CDS             complement(<1..>757)
                     /locus_tag="ACHU9J_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1907983.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSS"
BASE COUNT          141 a          255 c          174 g          187 t
ORIGIN      
        1 agagcttgat aaaaaatcta tagtttcttt catttgtgaa tcttgtttta aatactcaac
       61 agcctgatta aaatcacttg gatcaccgtt aacatggaat tttaaaccca aagaatctat
      121 aaaccgaaca gggtttaatg gatatttata cagattccat tcacctctca gccctatcgg
      181 atcctgggtg atataccttc cctgcaacgg gtcatagtac cggttgcggt tgtagtacag
      241 ccccgactcc tcatcatact gctgccccgg cagacggagt gactgctgga ggtgctgcgc
      301 gctggtttcg cccagcaggt ttccccattc gtcatactcc ccctgccacg ccgtttcccc
      361 ttccgggctg atgagcgcca gcggcagtcc ccggtggtcg cagtggtaaa gatgaagttt
      421 cctctccggg atgtattccg cctccagctg cgcggccatc tgctccgccg tcagcccgca
      481 ctgcgcaagc catgcctcac tctccgcact caccgctccc gcgcgcagtt cccgctccag
      541 cctccccagc atcaccgaca gctccgccgg tagcgtcaca cccgtgtcct cctgcaacac
      601 ctccgccagg ctccggtgcc gcgccttcgc ctgttcgcca ttctctgttt cgattctcag
      661 gagcggcgtg aagctccccg gctggtatac cgtctggata cgcgtggtgc cggtctgtat
      721 cgtcgtcagc ctgtcaccgt cccacccgta ccatgtc
//
LOCUS       DAZPJP010000473          755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPJP010000473 DAZPJP010000000
VERSION     DAZPJP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.476"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..77)
                     /locus_tag="ACHU9J_004661"
     CDS             complement(<1..77)
                     /locus_tag="ACHU9J_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1907984.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFL"
BASE COUNT          229 a          187 c          152 g          187 t
ORIGIN      
        1 tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc tgttgatatt
       61 ttttccatct gatacatatc ccgccccccg acagacctgt gctatcggaa agaaaaaagg
      121 ccatcagcaa aactctgatg gcctgaatca cctttaccaa tattgtatga aaaaacacgc
      181 acgactaatt gacaataatt ttcatttcca ttaaaaaaga tcgcgtagca ttcttaattc
      241 atgaggaact ttacccgcca gcaatctgag tagcgaaagc tgttcatccc caatgttttt
      301 gctggcgggt ccttttttct acggtcctcc tccccggagg ggggcatttc attatttttc
      361 taatcgttac tggtgagaag gcggaagaaa aagccagttc ttcgccccta aataacagca
      421 tgattccagt ccatgcaaaa tctgatttcc agctcacaca taccgagcgc tgtacaaaaa
      481 accgccagcc aaggcagtta agagtgtgtt cccggggttt gcttagaata ttttgtaagt
      541 tgtccgaaga gatatttaca acaccagaat gatgaatcat cagtcccctg ccagaggttc
      601 actacgtaac ctgaccgaca atgcaccatt ctggtgctga aaaaagagca ctggaactgc
      661 aacaaaacaa catcacagaa caggagatgg taaggagtca gacattccca cacaatattg
      721 tgtcagtgca aataacaacc ttcgtctcaa atctc
//
LOCUS       DAZPJP010000474          754 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPJP010000474 DAZPJP010000000
VERSION     DAZPJP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.477"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..722
                     /locus_tag="ACHU9J_004662"
     CDS             <1..722
                     /locus_tag="ACHU9J_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1907985.1"
                     /translation="DLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKP
                     LTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPY
                     LLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQT
                     ITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKH
                     LFAHGEYIKGITSLIKYKLKK"
BASE COUNT          238 a          145 c          147 g          224 t
ORIGIN      
        1 ctgatctgcc gacgtcacag ttttggtcgt acgcgacata cttcagggta ttgtcctttg
       61 aatatctgag tgaaagtatt tccacactgc tgtatctgga tgccgatgtc gtttgtaaag
      121 gaagtctgaa gcctctcaca gaaattatat ttaaagatga gtttgctgcg gtcattcctg
      181 acaatgttag tactcaggcg gcatgtgcaa aacgcctcaa cattcccgag atgaatggac
      241 gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa atggcatgaa gcaaatttga
      301 caccgtattt actcacgctt ttacgagggg aaactaaata tggctctctt aaatatttag
      361 atcaggatgc gttgaatatc gcatttaata tgaataatat ctacctcgcg aaggattttg
      421 atactattta taccctgaaa aacgaacttc atgatcgtag tcatcgaaag tatcagcaaa
      481 ccattaccga taaaacagta ttgattcact atacagggat aactaaacca tggcatagct
      541 gggctggtta tccgtctgca tcatacttta atatcgcgcg tgaacaatct ccctggaaga
      601 aatatcctct taaagaggcg cggactgttg cagaaatgca gaaacaatat aagcatctgt
      661 ttgcccatgg tgagtatatt aaaggcataa cttcattaat taagtacaag cttaagaaat
      721 aaattactct tatgattaca agtatacgct atcg
//
LOCUS       DAZPJP010000475          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPJP010000475 DAZPJP010000000
VERSION     DAZPJP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.478"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(55..351)
                     /locus_tag="ACHU9J_004663"
     CDS             complement(55..351)
                     /locus_tag="ACHU9J_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1907986.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          225 a          161 c          148 g          216 t
ORIGIN      
        1 ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattgaatct ctattcagaa
       61 cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta cgtacgggtc
      121 agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc
      181 agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac cagctgtcaa
      241 caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag
      301 gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac
      361 tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa
      421 gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa aatgacattt
      481 tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag
      541 acttggtttt tctactgaaa tgattatgac ttcaatgacc cttgaatgaa gtctattcgg
      601 ttttctagga gacctgttaa aaacaggaca atatcaggtg agtccctatg caatttaata
      661 gtagatttcc aagtatctat gcaattctac acagattcta tacagtctcg gtatagtccc
      721 tttttaccct aacttattga aatatataaa
//
LOCUS       DAZPJP010000476          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPJP010000476 DAZPJP010000000
VERSION     DAZPJP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.479"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHU9J_004664"
     CDS             <1..407
                     /locus_tag="ACHU9J_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1907987.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="ACHU9J_004665"
                     /pseudo
     CDS             449..649
                     /locus_tag="ACHU9J_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZPJP010000477          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPJP010000477 DAZPJP010000000
VERSION     DAZPJP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.480"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(154..705)
                     /locus_tag="ACHU9J_004666"
     CDS             complement(154..705)
                     /locus_tag="ACHU9J_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1907988.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT          201 a          196 c          121 g          228 t
ORIGIN      
        1 tcatcttccc cgaatcttcg aaaatatctg tctagttgat tcggtagaat ctacctcatg
       61 atgtatacgt gtttagtacc cgtcacacca gctatgtgac ccccttggca aaaaagccaa
      121 atatcagcca catccatgta actattcagt aacttattta gcaagtctgt aataagttgc
      181 tctagagatt cctagctctt cctgaatctg caacctcgtt ttaccttgtg aaataagcat
      241 ctgtacagcc tctgaagaag cttttacagg tcttcccata tgtacccctt cagctttagc
      301 acgttcaata ccagcctttc tacgctctgc tatcaagctt ctttcaagct gtgccaacgt
      361 tgacataatg cctaacattg cttcacccat agcgcttgat aagtcaaatc cttctctaag
      421 gctgataact gtcacacctt tagcctgtag cgctttaacc gttgataaca catcaattgt
      481 gtcacgtcca agacggtcta cagctaccac aactacggtg tcaccttctc gtgcataagc
      541 taacaaactg ctgaaaccgt ttcttaaaga agctttgaca gaaccactga cagcttcatc
      601 agcgaaccat ttagcaatgt tgtaccctgc ctcttcaatt tgcatttgct ggttagtgat
      661 ggattggtca tctgtactca ccctgcaata tccaagtatc gccataatgt tccccttaga
      721 agtctcaaaa gttagtctca taagtt
//
LOCUS       DAZPJP010000478          741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPJP010000478 DAZPJP010000000
VERSION     DAZPJP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.481"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(1..741)
                     /locus_tag="ACHU9J_004667"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="ACHU9J_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          197 a          195 c          160 g          189 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggatagg caaatggtgt attgccagtg acatcataac
      661 cagcaccgtg cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac
      721 catttaccat gattgctttc g
//
LOCUS       DAZPJP010000479          718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPJP010000479 DAZPJP010000000
VERSION     DAZPJP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.482"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            24..>718
                     /locus_tag="ACHU9J_004668"
     CDS             24..>718
                     /locus_tag="ACHU9J_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1907989.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          228 a          157 c          128 g          205 t
ORIGIN      
        1 gaatatcctt ctgcgaggtt attatgcttc ctgtaaataa tcccccccta tccactggaa
       61 acgtctcttt ttacagaact acatcaatcg acaatgttca caataattat ctctccgaat
      121 gggttgaatg gactaaaaac agcatttccg gagaaaacag ggaaactgct tttacccggc
      181 tccaattatg tctggagaac agtgaaacat cgttggactt atcttgttta ggtctcagat
      241 ctctaccacg attgcctgac aatcttgatg aaattaatgt aagcaataac caactatcaa
      301 tgctccccga gctaccaagg gcattgaaag agctgaatgc aagcagtaat caattatctg
      361 cacttcctga attaccagtg tcgctggaat atataaatgt gagtgataac catttgttcg
      421 cacttcctga attacctgcg tcactagaat atattaatgt aagtgacaat cacctgtctg
      481 tacttccgag gttaccaatg tcattggaat tacttgatgc agccagaaat gctttggaag
      541 taataccaga ttttccagaa agagatgatc atattataag aatattctgg cttaatcaga
      601 accggatcac ggcaattccg gaaagcatac ttggcctcag ttctgatagc gttgtcaatc
      661 ttagagaaaa tcaactatct cccagaataa tgcaaacttt gttacaacaa accgccca
//
LOCUS       DAZPJP010000480          714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPJP010000480 DAZPJP010000000
VERSION     DAZPJP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.483"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
BASE COUNT          197 a          140 c          148 g          229 t
ORIGIN      
        1 cttctgatgt ttaattgatt tgaatgttcg taagctatat cacttactca atccatttta
       61 cccagagtca taaaatagag tgtctgttga tctcattttc atattccttt cagactgaag
      121 tgcgttttca tcacccgatg agaaagaact gaagtgatgt ttgccataga ttgcgagatg
      181 ccctgtctca ttgactatcg cggtgcacct ttatacctgt tataccagat caaaaatcac
      241 gcaagccata caacaaaacg agatttgcaa ttcgtgtcac aaaatatgtc gatctttttc
      301 taagaggaag atgccatgtg aagccagacg aacacttgag gtggtcttca aaaactaaag
      361 gatttctttt aactgtttgt tttataagaa atttatttag ggtctaaaca agggaagctt
      421 tgcaagctaa ctcagtgagc gtggtgaaaa caagtgttta cccgccatca ggctgatcat
      481 aattctcacc atgaaatatg tttcctgatt tgtggcttgt aactggtcgc ttctgaagtc
      541 gatctagaga ggcttgttga tgttggtgtt ttcaggatga tgtttcactt agtttgtttg
      601 ccgtatcgcc cggcgaatag ctgtgattga ggaaggttta agtcgcagtg accaaagcca
      661 tatttaccca cgaatgtaga tgaaaaagcg tctcctgcgt tcccccttat ctcc
//
LOCUS       DAZPJP010000481          707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPJP010000481 DAZPJP010000000
VERSION     DAZPJP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.484"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            282..686
                     /gene="stpA"
                     /locus_tag="ACHU9J_004669"
     CDS             282..686
                     /gene="stpA"
                     /locus_tag="ACHU9J_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1907990.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          213 a          140 c          158 g          196 t
ORIGIN      
        1 tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggaatta
       61 gcgagcagag agcgccgttc tgttcaccaa taatctaaat tactcatcaa aatggctaat
      121 atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca
      181 agactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac
      241 gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa
      301 gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg
      361 aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc
      421 agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaagg
      481 ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgcgctg
      541 gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac cgatgttaac ggtgaaacta
      601 aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta
      661 aatctctcga cgatttcctg atctaattac tgaggccgga tgtcgct
//
LOCUS       DAZPJP010000482          701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPJP010000482 DAZPJP010000000
VERSION     DAZPJP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.485"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..211
                     /locus_tag="ACHU9J_004670"
     CDS             <1..211
                     /locus_tag="ACHU9J_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1907991.1"
                     /translation="TSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAWDAETSIPAL
                     LNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            316..507
                     /locus_tag="ACHU9J_004671"
     CDS             316..507
                     /locus_tag="ACHU9J_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1907992.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(508..>701)
                     /locus_tag="ACHU9J_004672"
     CDS             complement(508..>701)
                     /locus_tag="ACHU9J_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1907993.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
BASE COUNT          178 a          177 c          153 g          193 t
ORIGIN      
        1 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgttaaccat
       61 tccttgtaaa tctgaccccg atttatgtat gcaactcgat gcctgggatg ctgaaaccag
      121 tatccctgcc ctgctaaatg gcgaacactc tgtcctttac cgtacccgtt acgatcaaca
      181 atctgatgcc tggattatgc gtcttgcctg atccaaaaag aacccgtcgg gatggcgggt
      241 tatttgtcct ggttattccc ccgttgtaaa atctctccta aacttaacgg tacggcacca
      301 cacttcgggg atgaaatgtt cgcgctggta ctttttgttt gctacctgga tggcggttgt
      361 gaagatattg ttgtggatgt ctacaacacg gaacagcagt gtctttattc tatgagcgat
      421 caacggatcc gccatggcgg ttgttttccg attgaggatt ttatagatgg tttctggcga
      481 cctgcacagg agtacggtga tttttaatta ttgcaattgc acaagagtca gttcgccccc
      541 aaagacagca ccggtatcaa tataatgcag gttgccaata tccacgcgat gtcgcaacgg
      601 tgtatgacca aaccagaaat gatcagcacc tgtaattccc tgcccttttt ggcgttcacc
      661 taatcgcgag cggctccaca agacctgatg caaatcaacg t
//
LOCUS       DAZPJP010000483          700 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPJP010000483 DAZPJP010000000
VERSION     DAZPJP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.486"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            106..>700
                     /gene="ais"
                     /locus_tag="ACHU9J_004673"
     CDS             106..>700
                     /gene="ais"
                     /locus_tag="ACHU9J_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1907994.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFV"
BASE COUNT          192 a          146 c          154 g          208 t
ORIGIN      
        1 acatcgtagg atttttaata ttacaccaac tgctaattat cgccagaaat gtcgctcaat
       61 ttcacggtaa ttgtctggtt gcgcttgtct ataggtggag tttacgtgtt agctttttgc
      121 cgctcatcat tgaagtcaaa aaaatatttc atcattttac tggcgctcgc tgcaattgcc
      181 ggactgggta ctcatgccgc ctggagtagc aatggtttgc cacgtatcga caataaaaca
      241 ttggccagac tggcacagca gcacccggtt gtcgttttgt ttcgccatgc tgaacgttgc
      301 gatcgttcaa ccaatcaatg cttgtcagat aaaacaggta ttacggttaa aggtacccag
      361 gatgcccgtg aactgggcaa cgcttttagc gctgacatcc ctgatttcga tctttattcc
      421 agtaataccg tccggaccat tcagtcggcg acctggtttt ccgcgggtaa aaaactgacg
      481 gtagataaac gacttcttca gtgcggtaat gagatttata gtgcaattaa ggacttacaa
      541 agcaaagcgc ctgataaaaa tatcgttatt ttcacccata atcattgcct gacatatatt
      601 gctaaaaata agcgtgatgc gacatttaaa cctgattatc tggatggttt agtcatgcat
      661 gtggaaaaag gcaaagttta tctggatggg gaattcgtta
//
LOCUS       DAZPJP010000484          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPJP010000484 DAZPJP010000000
VERSION     DAZPJP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.487"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHU9J_004674"
     CDS             <1..>698
                     /locus_tag="ACHU9J_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907995.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZPJP010000485          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPJP010000485 DAZPJP010000000
VERSION     DAZPJP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.488"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHU9J_004675"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHU9J_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1907996.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
BASE COUNT          161 a          144 c          175 g          218 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgata
//
LOCUS       DAZPJP010000486          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPJP010000486 DAZPJP010000000
VERSION     DAZPJP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.489"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHU9J_004676"
     CDS             <1..>695
                     /locus_tag="ACHU9J_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1907997.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPJP010000487          691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPJP010000487 DAZPJP010000000
VERSION     DAZPJP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.490"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            149..573
                     /gene="yqeK"
                     /locus_tag="ACHU9J_004677"
                     /pseudo
     CDS             149..573
                     /gene="yqeK"
                     /locus_tag="ACHU9J_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            609..689
                     /locus_tag="ACHU9J_004678"
     CDS             609..689
                     /locus_tag="ACHU9J_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1907998.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          233 a           95 c          130 g          233 t
ORIGIN      
        1 ttcatttgat caaggttatc accttatgga gacgttacgt agaatgacta tatttgttat
       61 cggcacatta ggggtattaa ttttagtatt gatggaaggt gattattgaa cagtaacatc
      121 gagtcacggt aaaaatgtga ggtatacaat ggacattgag ttttcgcaga ttcatgaaat
      181 ggtctatatg catgatattg taaattcgga ctcgaaaaag aaaccaagga ttccgttaaa
      241 aaaattcctt aatgctgaga atgttctcac gcaaacaaca tcatggacat taaattcaag
      301 atatgtgaat gttaattctg tcaataaagt caacgtaaag agtaaagtta aaaacagtta
      361 tatctcacga tctgtcaatg atgagtttag tttaacagat gatgaaatca attcttttaa
      421 agaaacgtta gtcctttcct ctattgattc ctgagtaaac tggttcttaa taatcctctt
      481 tctgtattgt ttacatcgac tgtacgaagg aataataatc gagctaagat gaatgtcgag
      541 ttcgactcat ggatttgcac cagatgttgt tgatacatga tattttacat tgcaaggaga
      601 tgtctaaaat gaaagatgtt gatcaaaact ttgatgcttt agactgccat atactgcgag
      661 aatatttaat tttattgttt tatgattaac g
//
LOCUS       DAZPJP010000488          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPJP010000488 DAZPJP010000000
VERSION     DAZPJP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.491"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHU9J_004679"
     CDS             <1..560
                     /locus_tag="ACHU9J_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1907999.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHU9J_004680"
     CDS             complement(616..>684)
                     /locus_tag="ACHU9J_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908000.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPJP010000489          682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPJP010000489 DAZPJP010000000
VERSION     DAZPJP010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.492"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..573
                     /locus_tag="ACHU9J_004681"
     CDS             <1..573
                     /locus_tag="ACHU9J_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1908001.1"
                     /translation="SCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTL
                     AILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICH
                     EFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGC
                     SPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(616..>682)
                     /locus_tag="ACHU9J_004682"
     CDS             complement(616..>682)
                     /locus_tag="ACHU9J_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908002.1"
                     /translation="GTDEIMIYVAGRQILKDYQNK"
BASE COUNT          204 a          132 c          134 g          212 t
ORIGIN      
        1 tcttgcccgg taattgccgc agggcaggga aaagaagtca ttaaaacctt atttaatgag
       61 ctaagtgtga ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggatgcattt
      121 gcctatacat tagcaattct ttactacgaa aactttaaaa atgcttatcg ttcggagcag
      181 gggtatatta aaaaagatgt tctgataaaa gatattcttt tctatctgaa taataattat
      241 cgcgaaaaaa tcactttaga acagttatcg aaaaaatttc gtgccagcgt cagttatatt
      301 tgccatgaat ttaccaaaga gtatcgtatt tcccctatta actatgttat tcaacggcgt
      361 atgacggaag caaaatggtc actcactaat actgaattat cacaggcaga gatctcctgg
      421 cgtgtgggtt atgaaaatgt cgatcacttt gccaaactgt ttttgcgcca tgtcggttgt
      481 tcgcccagcg attaccgtag gcaatttaaa aattgttttg cggaacaaga aatcttatct
      541 gaatttcctc aaccggtaag tcttgccgga taaatctggc ggcgcacttt gttaaaaagc
      601 tgcgccgcct gcagattatt tgttctgata gtctttcagg atctgccgac ctgctacgta
      661 aatcataatt tcgtctgtac cg
//
LOCUS       DAZPJP010000490          670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPJP010000490 DAZPJP010000000
VERSION     DAZPJP010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.493"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHU9J_004683"
     CDS             <1..547
                     /locus_tag="ACHU9J_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908003.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          185 a          140 c          209 g          136 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag
//
LOCUS       DAZPJP010000491          651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPJP010000491 DAZPJP010000000
VERSION     DAZPJP010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.495"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>651
                     /locus_tag="ACHU9J_004684"
     CDS             <1..>651
                     /locus_tag="ACHU9J_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR1908004.1"
                     /translation="IGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLL
                     ERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWA
                     VTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEP
                     KNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          161 a          156 c          203 g          131 t
ORIGIN      
        1 ttatcggaga gctgcccacg tatggttatc gtcgggtatg ggcgctgctt cgcagacagg
       61 cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt ttaccggatc atgcgccaga
      121 atgcgctgtt gcttgagcga aaacctgctg taccgccatc gaaacgggca catacaggca
      181 gagtggccgt gaaagaaagc aatcagcgat ggtgctctga cgggttcgag ttctgctgtg
      241 ataacggaga gagactgcgt gtcacgttcg cgctggactg ctgtgatcgt gaggcactgc
      301 actgggcggt gactaccggc ggcttcaaca gtgaaacagt acaggacgtc atgctgggag
      361 cggtggaacg ccgcttcggc aacgatcttc cgtcgtctcc agtggagtgg ctgacggata
      421 atggttcatg ctaccgggct aatgaaacac gccagttcgc ccggatgttg ggacttgaac
      481 cgaagaacac ggcggtgcgg agtccggaga gtaacggaat agcagagagc ttcgtgaaaa
      541 cgataaagcg tgactacatc agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga
      601 accttgcaga ggcgttcgag cattataacg aatggcatcc gcatagtgcg c
//
LOCUS       DAZPJP010000492          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPJP010000492 DAZPJP010000000
VERSION     DAZPJP010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.496"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHU9J_004685"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHU9J_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHU9J_004686"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHU9J_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          154 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaacg
//
LOCUS       DAZPJP010000493          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPJP010000493 DAZPJP010000000
VERSION     DAZPJP010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.497"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHU9J_004687"
     CDS             complement(<1..>644)
                     /locus_tag="ACHU9J_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908005.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZPJP010000494          643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPJP010000494 DAZPJP010000000
VERSION     DAZPJP010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.498"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHU9J_004688"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHU9J_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          209 a          146 c          113 g          175 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataat
      541 ttccatggtt atttctaggc tatttaaatt aaataaatta tgagtcaatc ataaataaca
      601 acctttaata taatttaact accaaattta tattcgttca caa
//
LOCUS       DAZPJP010000495          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPJP010000495 DAZPJP010000000
VERSION     DAZPJP010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.499"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHU9J_004689"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHU9J_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1908006.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZPJP010000496          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPJP010000496 DAZPJP010000000
VERSION     DAZPJP010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.500"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            63..554
                     /locus_tag="ACHU9J_004690"
     CDS             63..554
                     /locus_tag="ACHU9J_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1908007.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKRENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          242 a           98 c          105 g          192 t
ORIGIN      
        1 aaaatattaa tgagtcaatc aataataatt tagcatcacg agacacatca cagaggaata
       61 ttatgagcac agaaacaatt gaaatattca ataatagtga tgaatgggca aatcaactaa
      121 aacacgcatt atcgaaaaga gaaaatctgg cattactaca tggtttaact cctgatatcc
      181 ttgatagaat atatgcatat gcattcgact accatgaaaa gggtaatata acggacgcag
      241 aaatttatta taaatttttg tgcatttatg cgttcgaaaa tcatgagtat ctaaaagatt
      301 ttgcatcagt atgtcagcca aaaaagaaat atcaacaagc atatgacctt tacaaactaa
      361 gttacaatta ctccccgtat gatgactatt cagttattta tcgtatgggt caatgtcaga
      421 ttggggctaa aaatatcgat aacgcaatgc aatgtttcta tcacattatt aacaattgtg
      481 aggatgatag tgttaagagt aaagcgcagg catatattga actcttaaac gataattcag
      541 aagataatgg ctaagcatta caatcatttg gttaaatttg ctggttatag tgcagttaac
      601 tactataacc agcacctctt atgttttctt aaatcag
//
LOCUS       DAZPJP010000497          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPJP010000497 DAZPJP010000000
VERSION     DAZPJP010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.501"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>637
                     /locus_tag="ACHU9J_004691"
     rRNA            <1..>637
                     /locus_tag="ACHU9J_004691"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZPJP010000498          637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPJP010000498 DAZPJP010000000
VERSION     DAZPJP010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.502"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>637)
                     /locus_tag="ACHU9J_004692"
     CDS             complement(<1..>637)
                     /locus_tag="ACHU9J_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1908008.1"
                     /translation="WVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKD
                     ATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDD
                     NSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRL
                     ADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKE"
BASE COUNT          197 a          137 c          105 g          198 t
ORIGIN      
        1 ttcctttgag gttgggtcaa acgacttcgc gaagctaatt aattgtggtg cagcatctcg
       61 cattttgctt aaaatatcag gatcgagcgt tccttcattc accagatgtg acatcttatc
      121 cagatagcca tcaaaattca tttctccccc atcagccaga cgcttcatct ctcccaggta
      181 cttcttcata tcacgttgag ataatttttc aaactgagct ttcaggtagc tctcattatt
      241 ttcattaata tacactgttt ctgataaatc tgcgtaggca ctagaatatg aaagtgaaat
      301 taacagcgaa gcaaataatt ttaagctgtt gtcatcatgt tcctggcagg cattaacaaa
      361 cgttaaaaaa ccagaaccta ttgcttgaaa atgtatattc gctaaaggtt cattatcctt
      421 agattcctca taaaatagct gaaccgtgga gggaaggcca ttcttagttt ctacatcaaa
      481 agtacattgt tcaatgggag tcgcatcttt attatcccca ggattacata tactattaat
      541 cgcgaaatga aaaatagccc ggtcaacaac tgatgcaata aagtcacgat cgtttaaatc
      601 ctcatcagtg caatcgtcaa tatacttatt gacccac
//
LOCUS       DAZPJP010000499          625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPJP010000499 DAZPJP010000000
VERSION     DAZPJP010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.503"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            52..625
                     /locus_tag="ACHU9J_004693"
                     /pseudo
     CDS             52..625
                     /locus_tag="ACHU9J_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          195 a          152 c          160 g          118 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagca
//
LOCUS       DAZPJP010000500          610 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPJP010000500 DAZPJP010000000
VERSION     DAZPJP010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.504"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>610
                     /locus_tag="ACHU9J_004694"
     CDS             <1..>610
                     /locus_tag="ACHU9J_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1908009.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRL"
BASE COUNT          125 a          176 c          171 g          138 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc
//
LOCUS       DAZPJP010000501          607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPJP010000501 DAZPJP010000000
VERSION     DAZPJP010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.505"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>607
                     /locus_tag="ACHU9J_004695"
     rRNA            <1..>607
                     /locus_tag="ACHU9J_004695"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          130 c          188 g          117 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaa
//
LOCUS       DAZPJP010000502          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPJP010000502 DAZPJP010000000
VERSION     DAZPJP010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.506"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            12..551
                     /locus_tag="ACHU9J_004696"
     CDS             12..551
                     /locus_tag="ACHU9J_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR1908010.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT          153 a          118 c          161 g          172 t
ORIGIN      
        1 aggagaatca gatgaactgg cattatgaaa aaaatggtgt acgttatgat aacgtaacag
       61 aggaagatat tatcgaacgt atccggcgtg gtgaactgaa tgcatcaaca ctggtgtggc
      121 aacagggcat gctggaatgg cagccagttt cagccacatc actggcggaa atactgaaac
      181 aaagtgaagt tcccccagcc cttccgggtc accgcattcc cggaggcgtt gtctggacgc
      241 tggcatttgc ccctttgatt gggtacatac ttgagatgtg gactgcaggg ctgaacggta
      301 tgtcatttga acgggcttat gatgcagtca gcgaaggcca gtactggttt atctctctga
      361 ttctgaatat tgctctggga tatctggatg aacgaaaact ccgtaaatct ggtgttgata
      421 ctgcaacatt tggctggctg gcatggttga tcccggttta tctgtggcgt cgggcaaaaa
      481 tcctcggaca aaaacctgcg tatttctggg tctggttggt tatgctgata ctggtgttat
      541 ttacggtctg atattcttta tcgcagagtt tactaaataa tcaggttatt agtgttctta
      601 acag
//
LOCUS       DAZPJP010000503          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPJP010000503 DAZPJP010000000
VERSION     DAZPJP010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.507"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHU9J_004697"
     CDS             <1..91
                     /locus_tag="ACHU9J_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908011.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHU9J_004698"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHU9J_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1908012.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPJP010000504          588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPJP010000504 DAZPJP010000000
VERSION     DAZPJP010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.508"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>588
                     /gene="espL4"
                     /locus_tag="ACHU9J_004699"
     CDS             <1..>588
                     /gene="espL4"
                     /locus_tag="ACHU9J_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFR1908013.1"
                     /translation="RIKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDY
                     SSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAA
                     ILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFM
                     NVDYSLLKWSNDHVINQSVAIIPALPKEQLLILKG"
BASE COUNT          195 a          109 c          115 g          169 t
ORIGIN      
        1 caagaattaa acttaatggt caatgttatt ttccggggcg accccaaaac cgtatagtat
       61 gccgacacat tgctgcacaa tatattaatg atatttatca gaatgttgat tacaaacccc
      121 atcaagatga ttattcttca gctgaaaaat ttctcactca cttcaacaaa aaatgcaaaa
      181 accagacttt ggcgttgatt tccagccgtc ctgaggggcg ttgcgttgct gcctgcggtg
      241 atttcgggct agttatgaaa gcatattttg acaagatgga atcaaatggc atcagtgtta
      301 tggcagccat attactggtg gataaccatg ctttgacggt ccggctaaga ataaagaaca
      361 caactgaagg atgtacccat tacgtggttt cggtttatga tcctaatgta actaacgaca
      421 aaataagaat catgagcgaa agcaaagagg atattaaaca ctattctctg atggatttta
      481 tgaatgtaga ttatagcctc ctgaaatggt caaatgatca tgttattaac caatctgttg
      541 caataattcc agcacttccg aaagaacagc tattgatatt aaaaggat
//
LOCUS       DAZPJP010000505          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPJP010000505 DAZPJP010000000
VERSION     DAZPJP010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.509"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            2..>575
                     /gene="istB"
                     /locus_tag="ACHU9J_004700"
     CDS             2..>575
                     /gene="istB"
                     /locus_tag="ACHU9J_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1908014.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYG"
BASE COUNT          169 a          128 c          167 g          111 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacaga tgagggaact gctgaacggc cactacataa tccacaggaa
      301 aaacctactg ataaccggtc cgacaggatg cggtaagagc tggatagcca atgcgctggg
      361 cgaacaggca tgtcgacaga aatacagcgt ccggtactgt agaaccgggc gattactgga
      421 acagttggct cagggacgtg ttgatggcag ttggctgaaa tatctgaaac agttgcaaaa
      481 aatacaggtt ctaatcctgg acgatctggg gctggaacag ttaagtaacg cccagtgtaa
      541 tgaccttctg gagatcacag aggatcgtta cggac
//
LOCUS       DAZPJP010000506          563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPJP010000506 DAZPJP010000000
VERSION     DAZPJP010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.510"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..563
                     /locus_tag="ACHU9J_004701"
                     /pseudo
     CDS             1..563
                     /locus_tag="ACHU9J_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          176 a          111 c          112 g          164 t
ORIGIN      
        1 aacaacctga tcacataatc gggtacgatt ttatccgata actataatgc tataactctg
       61 ggaaatggtt ggaatacacc gctgggtgcc atctcttttt acgctgcccg atcgagtagt
      121 aagttaaata atgataccag acacgaaggt acgagctatc aggtcgccta taataaatat
      181 ctagtacaaa cagccactcg atttagcgtg gccgcctggc gttatgcttc gcaggattac
      241 aggacattca gcgaccatct ttacgaaaat gataaacact atcatcagag tgattatgat
      301 gatttttatg atattggcag aaaaaatagc ctttctgcca atattatgca acctttatcc
      361 aataatctgg gaaatgtatc attaagtgcg ctttggcgga attactgggg gcgaagtgga
      421 aatgctaaag attaccaatt cagttactcc aataactggc aacacattag ttatactttc
      481 tctgccagcc aatcttatga tgaaaataat aaagaagagg agcgttttaa tctgtttatc
      541 tccattcctt tctactgggg gga
//
LOCUS       DAZPJP010000507          562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPJP010000507 DAZPJP010000000
VERSION     DAZPJP010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.511"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHU9J_004702"
     CDS             <1..118
                     /locus_tag="ACHU9J_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1908015.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNCEKLQSS"
     gene            225..485
                     /locus_tag="ACHU9J_004703"
     CDS             225..485
                     /locus_tag="ACHU9J_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1908016.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMGDAANLLI"
BASE COUNT          176 a          115 c          129 g          142 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaatggg tgatgctgcc aacttactga
      481 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
      541 tcctgttcag ctactgacgg gg
//
LOCUS       DAZPJP010000508          562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPJP010000508 DAZPJP010000000
VERSION     DAZPJP010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.512"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..426
                     /locus_tag="ACHU9J_004704"
     CDS             <1..426
                     /locus_tag="ACHU9J_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908017.1"
                     /translation="SNNSAIYFAANKFIVSGTDTATVGGTAPFAIVNGTTYLKTAMIQ
                     AASIGTGYIADAAITNLKLASGSVNTLNVVDGCITTAKISNTIQSTNYVANKTGWQIN
                     KAGTILINGTGGTGRMVISNNIIQIYDNNNTLRVRMGLW"
BASE COUNT          202 a          102 c          108 g          150 t
ORIGIN      
        1 agtaacaact cagcaatcta tttcgctgcg aacaagttca tagtctccgg tactgacact
       61 gctactgtag gtggtactgc accatttgcc atagtcaacg gtactacata cttgaagacg
      121 gcaatgatac aggcggcaag tatcggtact ggttacatcg ctgatgcggc aattaccaat
      181 ctaaaactgg cctccggctc agtgaataca ttaaacgtgg ttgatggttg tataactaca
      241 gccaaaatct caaatactat tcaaagtact aattacgtag caaataaaac aggctggcaa
      301 atcaataaag caggtacgat cctaatcaat ggaacgggtg gtactggtag aatggtaatc
      361 agtaataata ttattcaaat ctatgacaat aataatacgt tacgtgtacg tatgggttta
      421 tggtaataaa taattcaggg attattaact gtaacctcat ttcaaaagga tacataaatg
      481 aaatctaatg aacgtacttt caatagtacc agtactaacc cattcagtat tagcggaaaa
      541 gaaacaattg gaatgaagaa aa
//
LOCUS       DAZPJP010000509          560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPJP010000509 DAZPJP010000000
VERSION     DAZPJP010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.513"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>560)
                     /locus_tag="ACHU9J_004705"
     CDS             complement(<1..>560)
                     /locus_tag="ACHU9J_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR1908018.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          119 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact
//
LOCUS       DAZPJP010000510          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPJP010000510 DAZPJP010000000
VERSION     DAZPJP010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.514"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            256..>559
                     /locus_tag="ACHU9J_004706"
     CDS             256..>559
                     /locus_tag="ACHU9J_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1908019.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          126 a          139 c          131 g          163 t
ORIGIN      
        1 ttttttatta ctcgctgtaa tgtacgagcc tgtcgtgaat aacgtcgaga acccatccgt
       61 tgcgtttgtg attattttgt tgactaaaca gacacccgtt tctctgaagt aaaatcccag
      121 actaaatcat cacataacca tgacattttt ctgatattcc ccggtaacgc cagatgtcga
      181 ctcgcttatc tcatccgccc gacatgttgt acatgcaata tgtgcggatt attattcatt
      241 ttcacggagg ttgctatgtc aaccggatta cgtttcacac tggaagtgga cggcctgcca
      301 ccggatgctt ttgcggtggt ttcctttcat ttgaaccagt cactctcttc gctcttttcc
      361 ctcgatctct ctctggtcag ccagcagttt ctctcccttg aatttgcgca ggtgctggac
      421 aaaatggcct acctgacggt atggcagggc gatgacgtac agcgccgggt gaaaggtgtg
      481 gtgacctggt ttgaactggg ggagaacgac aaaaaccaga tgctgtacag catgaaggta
      541 tgcccaccgc tgtggcgca
//
LOCUS       DAZPJP010000511          556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPJP010000511 DAZPJP010000000
VERSION     DAZPJP010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.515"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            78..556
                     /locus_tag="ACHU9J_004707"
                     /pseudo
     CDS             78..556
                     /locus_tag="ACHU9J_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          176 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagcat
//
LOCUS       DAZPJP010000512          556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPJP010000512 DAZPJP010000000
VERSION     DAZPJP010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.516"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>556
                     /gene="feaR"
                     /locus_tag="ACHU9J_004708"
     CDS             <1..>556
                     /gene="feaR"
                     /locus_tag="ACHU9J_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR1908020.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          143 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aatata
//
LOCUS       DAZPJP010000513          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPJP010000513 DAZPJP010000000
VERSION     DAZPJP010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.517"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHU9J_004709"
     CDS             <1..507
                     /locus_tag="ACHU9J_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1908021.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
BASE COUNT          199 a           95 c          100 g          157 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg t
//
LOCUS       DAZPJP010000514          550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPJP010000514 DAZPJP010000000
VERSION     DAZPJP010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.518"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..550
                     /locus_tag="ACHU9J_004710"
                     /pseudo
     CDS             1..550
                     /locus_tag="ACHU9J_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          133 a          137 c          162 g          118 t
ORIGIN      
        1 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
       61 caactgcacg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
      121 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
      181 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
      241 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
      301 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
      361 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
      421 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
      481 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
      541 aaaacactat
//
LOCUS       DAZPJP010000515          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZPJP010000515 DAZPJP010000000
VERSION     DAZPJP010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.519"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHU9J_004711"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHU9J_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPJP010000516          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZPJP010000516 DAZPJP010000000
VERSION     DAZPJP010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.520"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHU9J_004712"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHU9J_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZPJP010000517          524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPJP010000517 DAZPJP010000000
VERSION     DAZPJP010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.521"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(261..>524)
                     /locus_tag="ACHU9J_004713"
     CDS             complement(261..>524)
                     /locus_tag="ACHU9J_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1908022.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHITINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          130 a          127 c           82 g          185 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag
      301 tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca
      361 ttcgtcaaaa tattaatagt aatgtgccgt atccctgaaa ataattctgc tgcatttcct
      421 cttcttattt tgcagtcgtc ttcattcatt accacgtcca gacgccagtg cagcttattc
      481 tccacgtgcc agtggtttcg gattgctgtg gcgaactttt ctgc
//
LOCUS       DAZPJP010000518          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPJP010000518 DAZPJP010000000
VERSION     DAZPJP010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.522"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>522)
                     /locus_tag="ACHU9J_004714"
     CDS             complement(<1..>522)
                     /locus_tag="ACHU9J_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908023.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAZPJP010000519          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZPJP010000519 DAZPJP010000000
VERSION     DAZPJP010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.523"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>522
                     /gene="espX1"
                     /locus_tag="ACHU9J_004715"
     CDS             <1..>522
                     /gene="espX1"
                     /locus_tag="ACHU9J_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR1908024.1"
                     /translation="KHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLKANFN
                     QEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIF
                     PELYNEQCNKIPSSSIDDNQGIMWQNFKTSNSAYSKLCGKLSLFKSVPSRLIEKSAYC
                     STENMATNKFNVV"
BASE COUNT          208 a           94 c           79 g          141 t
ORIGIN      
        1 taaacacaaa ggtatcacat tacttagatt ggatgttttt gatgacagaa caagcgaatg
       61 tctttctgaa gagattaaga tataccaaga atgccatgaa aaattcgtta attttttaaa
      121 ggcaaacttc aaccaagaaa tatacccaca gctatatact cctgaaatat tttatgaggc
      181 ctgcagaaat ctccagtcat tttatgatca tcaggaaacg tcacaaaacg caaaatacag
      241 tgcaatagac aagaaaaaaa gtcatcttaa ccaggaaatc aaaaagctta ttaaaaaaaa
      301 tattttccct gagctataca atgagcaatg caataaaatc ccctcctcat cgatagatga
      361 taaccaggga ataatgtggc agaactttaa aactagcaat tcggcatatt caaaactatg
      421 tggaaaatta tctttattta aaagcgtgcc cagtcggtta atagaaaaat cagcttactg
      481 ctcaactgaa aacatggcca ctaataaatt taatgtcgtc tt
//
LOCUS       DAZPJP010000520          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPJP010000520 DAZPJP010000000
VERSION     DAZPJP010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.524"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>511
                     /locus_tag="ACHU9J_004716"
     CDS             <1..>511
                     /locus_tag="ACHU9J_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908025.1"
                     /translation="EGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSE
                     QGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYEL
                     ATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTT
                     AANLDIDIP"
BASE COUNT          109 a          147 c          187 g           68 t
ORIGIN      
        1 gtgaaggcca ccgtgtggcg gtccactacg gctatgacga taaaggccgt ctgacgggcg
       61 aacggcagac ggtggagacc ccggagacgg gggagatgct gtgggagcat gagacggggc
      121 acgcgtacag cgaacagggg ctggcgaccc gtcaggagcc ggacggcctg ccgccggtgg
      181 agtggctgac gtatggcagc ggttatcttg cggggatgaa gctgggcgga acaccgctgg
      241 tcgagtacac gcgggaccgg ctgcaccgtg agacggcccg cagcttcggc ggggaggcat
      301 atgaacttgc caccgcctgg aataccagcg gccagctccg gagcaggcac ctgaaccttc
      361 cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc atcagtggcc
      421 cgcaggagag ccgggagtac cgttacagcg acacggggag gctgacgggc gtccacacca
      481 cggcagcgaa cctggatatc gatatcccgt a
//
LOCUS       DAZPJP010000521          500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPJP010000521 DAZPJP010000000
VERSION     DAZPJP010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.525"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..121
                     /locus_tag="ACHU9J_004717"
     CDS             <1..121
                     /locus_tag="ACHU9J_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1908026.1"
                     /translation="YIKGACDQLCRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            118..432
                     /locus_tag="ACHU9J_004718"
                     /pseudo
     CDS             118..432
                     /locus_tag="ACHU9J_004718"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            380..>500
                     /locus_tag="ACHU9J_004719"
     CDS             380..>500
                     /locus_tag="ACHU9J_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1908027.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET"
BASE COUNT          130 a          126 c          137 g          107 t
ORIGIN      
        1 ctatatcaaa ggcgcatgtg accagctatg tcgctggaca tatgctggcg gtaagcaatg
       61 gaaagggctg atgacccgtc gtgagattga gcgtgaagtc tgtttgtggg ggcagcaatg
      121 agcagagtca ccgcgattat ctccgctctg gttatctgca tcatcgtccg cctgtcatgg
      181 gctgttaatc attaccgtga taacgccatt acctacaaag agcagcgcga caaaaacgca
      241 agagaactga agctggcgaa cgcaaccatt actgacatgc agcagcgcca gcgtgatgct
      301 gatgcactcg atgctaaata cacgaaggag ttagctgatg cgaaagctga aaatgatgct
      361 cttcggcgca agcttgataa tggtggtcgg gtgctcgtca aaggaaaatg ccctgtgcca
      421 tcctcagccg aaacctccag cgcctccggc atgggcaatg atgccaccgt cgaactctct
      481 ccagttgctg gacgaaacgt
//
LOCUS       DAZPJP010000522          490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPJP010000522 DAZPJP010000000
VERSION     DAZPJP010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.527"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHU9J_004720"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHU9J_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1908028.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          111 a          136 c          134 g          109 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc
//
LOCUS       DAZPJP010000523          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPJP010000523 DAZPJP010000000
VERSION     DAZPJP010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.528"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU9J_004721"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHU9J_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1908029.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPJP010000524          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPJP010000524 DAZPJP010000000
VERSION     DAZPJP010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.529"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHU9J_004722"
     rRNA            <1..>476
                     /locus_tag="ACHU9J_004722"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPJP010000525          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPJP010000525 DAZPJP010000000
VERSION     DAZPJP010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.530"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHU9J_004723"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHU9J_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          109 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa accctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPJP010000526          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPJP010000526 DAZPJP010000000
VERSION     DAZPJP010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.531"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>463
                     /locus_tag="ACHU9J_004724"
     CDS             <1..>463
                     /locus_tag="ACHU9J_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1908030.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          119 a          108 c          149 g           87 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cac
//
LOCUS       DAZPJP010000527          459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZPJP010000527 DAZPJP010000000
VERSION     DAZPJP010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.532"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHU9J_004725"
     CDS             2..388
                     /locus_tag="ACHU9J_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1908031.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           96 c          123 g          105 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagcactg
//
LOCUS       DAZPJP010000528          447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPJP010000528 DAZPJP010000000
VERSION     DAZPJP010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.534"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(72..>447)
                     /locus_tag="ACHU9J_004726"
     CDS             complement(72..>447)
                     /locus_tag="ACHU9J_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908032.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT          138 a           99 c           70 g          140 t
ORIGIN      
        1 gagaaggtaa agaaggtaag tccatactgt ctccactata aaaaaaccgc caatttctgg
       61 cggcttatat attaatcatc attaatatct ttcatattca atttgcaatt ttcgtaggtc
      121 actcccgact cgccattaac attaactcca gctctgatat tcccgttttc ttttacatct
      181 acaaaaaact ggcctcttgg catcttgaac gcaaatgtaa aaccaacagt atctgaatca
      241 aaatcatcat ctttatatat tgggatcaaa aatgtcatcg tcgctggtga tgcattgttt
      301 gcaaaatcaa atacacctat acttttgccg aattcataag aagcttttct caatataatc
      361 gcaccgctgg cagaagggga tgggcacgat atcctcagtt gtctgaatcc atctgtatcc
      421 aagtcaacgt cattatctgc agatgtt
//
LOCUS       DAZPJP010000529          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPJP010000529 DAZPJP010000000
VERSION     DAZPJP010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.535"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..444
                     /locus_tag="ACHU9J_004727"
                     /pseudo
     CDS             1..444
                     /locus_tag="ACHU9J_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          131 a           88 c           96 g          129 t
ORIGIN      
        1 acaaaacaag attaataaat ttaataatta ccttttccgc tggcagggcc tggatggctc
       61 agaaattctg atgcataact ttcctgaaga cacttatgat agccgagcac gtgctcgctc
      121 tttagaatat attgaacaaa actataatga aaaagagatc tgtccctatg cgcttatggt
      181 gtatggtgta tggtgtcggt gatggcggcg ctggccccgg tgaggaacat atagaacgat
      241 taacacgtat acgcaatatt gatggcttac ctcatgttga tttcagccgt gttgataagt
      301 tcttcactta cgccgacgcc tttcgcgaaa gcttgcccat tatttcaggt gaactttatt
      361 tcgaggctca tcaagggtgc tttacatcag agtcagcaac taaagctcat aatcgaaata
      421 tggaaaataa attacatgat gctg
//
LOCUS       DAZPJP010000530          444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPJP010000530 DAZPJP010000000
VERSION     DAZPJP010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.536"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..399
                     /locus_tag="ACHU9J_004728"
     CDS             <1..399
                     /locus_tag="ACHU9J_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFR1908033.1"
                     /translation="LAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRN
                     IKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYS
                     SYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT          177 a           57 c           89 g          121 t
ORIGIN      
        1 ttggcaatgt gtaatggaga atcaattaac gagaataaag aatatggtgg actaatatgt
       61 aagaagcaag gtgaatattt ccccatgaat ccgataagtt caaatgataa tgatagtgta
      121 gacttgcgaa atataaaatg ccctgaaggt tcagagagag taggcgatta tcacactcac
      181 ggtttttact ctgacgataa aggaaataaa gtaacaaaag aaaatgatgt ttatgatagt
      241 ctaaattttt caagcaaaga tttaacgaat tcttatatga atggaatggg aaaaaaagaa
      301 tacagtagtt acttgggaac accaaataac acctatctaa aatataatcc caaagctaaa
      361 gggaatggag ttacaattat caggcaaggg agtaattaaa atgaaggcaa tacaatgttg
      421 ttatttcctt gctttagtct ccag
//
LOCUS       DAZPJP010000531          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPJP010000531 DAZPJP010000000
VERSION     DAZPJP010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.537"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHU9J_004729"
     CDS             <1..>442
                     /locus_tag="ACHU9J_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1908034.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZPJP010000532          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPJP010000532 DAZPJP010000000
VERSION     DAZPJP010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.538"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..438
                     /locus_tag="ACHU9J_004730"
                     /pseudo
     CDS             1..438
                     /locus_tag="ACHU9J_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          108 a          116 c          141 g           73 t
ORIGIN      
        1 cacacgtagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc ggacggcagc
       61 gtcacgcaga gtcagtttga cgccgtgggc aggctcagga cacagacgga tgccgcaggc
      121 cggacaacag aatacagccc ggatgtggtg acgggtctca tcacgcgcat caccacgccg
      181 gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc agccaccggg
      241 cctgacgggc tggaaatacg ccgggaatat gatgaatggg gcaacctgct gaatgaagag
      301 aacccgcatc agctgcagca gcttattcgt ttgccggggc agcagtatga tgaggagtcc
      361 ggcctgtatt ataaccgcca ccgctattat gacccgctgc aggggcggta tatcactcag
      421 gatccgattg ggctgaag
//
LOCUS       DAZPJP010000533          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPJP010000533 DAZPJP010000000
VERSION     DAZPJP010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.539"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHU9J_004731"
     CDS             <1..187
                     /locus_tag="ACHU9J_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1908035.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          132 a           99 c          107 g           96 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tgattcaccg
      361 tcggatttac tgctacgtcg ccctcaggga attaaaccta aaaagaaaat cgtcaaaatt
      421 agagattccg gttt
//
LOCUS       DAZPJP010000534          433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZPJP010000534 DAZPJP010000000
VERSION     DAZPJP010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.540"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHU9J_004732"
     CDS             complement(<1..112)
                     /locus_tag="ACHU9J_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908036.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>433)
                     /locus_tag="ACHU9J_004733"
     CDS             complement(368..>433)
                     /locus_tag="ACHU9J_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR1908037.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          126 a           89 c           86 g          132 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caa
//
LOCUS       DAZPJP010000535          429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZPJP010000535 DAZPJP010000000
VERSION     DAZPJP010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.541"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(133..>429)
                     /locus_tag="ACHU9J_004734"
     CDS             complement(133..>429)
                     /locus_tag="ACHU9J_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR1908038.1"
                     /translation="ALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLIS
                     IPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          123 a          121 c          102 g           83 t
ORIGIN      
        1 gccccctgcg cgctccttgc gccagtggca atatgtggct tagctcatga aaggagcgca
       61 acaagatgat gaatcaacat ataacaacat cttaaaaaaa ggcctgacat tacgccaggc
      121 cttctgcgtt aattaatcac gcttttccag cagggtccgg taaatcagac caccgataat
      181 gccgccgaca attggcacca cccagaagaa ccacagttgt tctaatgccc agccgccctg
      241 gaagatagca accgcggtgc tgcgcgccgg gttaacagaa gtgttagtca ccggaatact
      301 aattaagtga atcagggtta aggccagacc aatagcgatc ggcgcaaaac ctgccggcgc
      361 gaatttgtcg gttgcgccgt ggatcaccaa caggaaacct gcactcaata ccagttcaac
      421 taccagcgc
//
LOCUS       DAZPJP010000536          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZPJP010000536 DAZPJP010000000
VERSION     DAZPJP010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.542"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>427
                     /locus_tag="ACHU9J_004735"
     CDS             <1..>427
                     /locus_tag="ACHU9J_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR1908039.1"
                     /translation="IVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQF
                     QPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQK
                     RKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA"
BASE COUNT          101 a          105 c          128 g           93 t
ORIGIN      
        1 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
       61 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
      121 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
      181 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
      241 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
      301 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
      361 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
      421 gttgcat
//
LOCUS       DAZPJP010000537          413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZPJP010000537 DAZPJP010000000
VERSION     DAZPJP010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.543"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..78)
                     /locus_tag="ACHU9J_004736"
     CDS             complement(<1..78)
                     /locus_tag="ACHU9J_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908040.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKT"
     gene            96..287
                     /locus_tag="ACHU9J_004737"
     CDS             96..287
                     /locus_tag="ACHU9J_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1908041.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          100 a          105 c           80 g          128 t
ORIGIN      
        1 cgttttttta cataacgaag aattgaccat tttgtcctgt tgtgccttaa tgtaagtacc
       61 gtccacagcg tgggacatac ttcaaggaac cttttgtgag tcaggcaacc agtatgcgaa
      121 aacgacaccg atttaacagt cgcatgaccc gtatcgtact gctcatcagc tttatcttct
      181 tctttggccg ttttatctac tcgtccgtcg gtgcctggca gcaccatcag agcaaaaaag
      241 aagctcagca atccacactc tccgtcgaat caccggtaca acgttagcgg ttaccttctt
      301 cactttcaca gaacataacg gcacttcgct gtcggatgct tttgctcttt gggattatca
      361 aagcggcaga tattctttca tcttaaattt tacgtctttg tcctgactga tgt
//
LOCUS       DAZPJP010000538          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZPJP010000538 DAZPJP010000000
VERSION     DAZPJP010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.544"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>406
                     /locus_tag="ACHU9J_004738"
     rRNA            <1..>406
                     /locus_tag="ACHU9J_004738"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          109 a           91 c          119 g           87 t
ORIGIN      
        1 ggatggagct gaaatcagtc gaagatacca gctggctgca actgtttatt aaaaacacag
       61 cactgtgcaa acacgaaagt ggacgtatac ggtgtgacgc ctgcccggtg ccggaaggtt
      121 aattgatggg gtcagcgcaa gcgaagctct tgatcgaagc cccggtaaac ggcggccgta
      181 actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc tgcacgaatg
      241 gcgtaatgat ggccaggctg tctccacccg agactcagtg aaattgaact cgctgtgaag
      301 atgcagtgta cccgcggcaa gacggaaaga ccccgtgaac ctttactata gcttgacact
      361 gaacattgag ccttgatgtg taggataggt gggaggcttt gaagtg
//
LOCUS       DAZPJP010000539          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZPJP010000539 DAZPJP010000000
VERSION     DAZPJP010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.545"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>401
                     /locus_tag="ACHU9J_004739"
     CDS             <1..>401
                     /locus_tag="ACHU9J_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1908042.1"
                     /translation="GQSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRS
                     LNDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG
                     RINSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          112 g          119 t
ORIGIN      
        1 ggtcaatcta aaagtcttgg tgaatctctt ggtggcttga tgagcgatat gtcgcatggg
       61 gtcgattcac taaccgatat tagttacagg gtacgagcgt tctttgatga ggtgacatta
      121 gcttatcgtt cactaaatga cactcaaaaa gctgtagctg atggtcttgc aaatggcctg
      181 ttaagtgcat taaaggggct tgctggtata gtcgctgtac ggtctgggat tggtgctgta
      241 ggtggtatct ggaacttaat tagggccatt tccacgcttg gaaatgtggc aaatacagca
      301 gcaggaagaa tcaattcacg ttctactggt agtgttgaag gtggaaaaag taggctatct
      361 gtagctgatg caattaccag agtaatgatt gtcggtatga c
//
LOCUS       DAZPJP010000540          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZPJP010000540 DAZPJP010000000
VERSION     DAZPJP010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.546"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHU9J_004740"
     CDS             <1..>399
                     /locus_tag="ACHU9J_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1908043.1"
                     /translation="FLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIET
                     QDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQF
                     QAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          128 a           85 c           85 g          101 t
ORIGIN      
        1 tttctctata acaaatcgct caatgagtca ggcacgaata ttcagttagt gggttaccgt
       61 tattcgacca gtggatattt taatttcgct gatacaacat acagtcgaat gaatggctac
      121 aacatcgaaa cacaggatgg tgttattcag gttaagccga aattcaccga ctattacaat
      181 ctcgcttata acaaacgcgg gaaattacaa ctcaccgtta ctcagcaact cgggcgcaca
      241 tcaacactgt atttgagtgg tagccatcaa acttattggg gaacgagtaa tgtcgatgag
      301 caattccagg ctggtttaaa tactgcattc gaagatatca actggacgct cagctatagc
      361 ctgacgaaaa acgcctggca aaaaggacgg gatcagatg
//
LOCUS       DAZPJP010000541          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZPJP010000541 DAZPJP010000000
VERSION     DAZPJP010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.547"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..63
                     /locus_tag="ACHU9J_004741"
     CDS             <1..63
                     /locus_tag="ACHU9J_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908044.1"
                     /translation="GKKLVAGMDVNIDDEIGDDE"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHU9J_004742"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHU9J_004742"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1908045.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKEKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa ggaaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZPJP010000542          392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZPJP010000542 DAZPJP010000000
VERSION     DAZPJP010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.548"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
BASE COUNT          147 a           57 c           86 g          102 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tt
//
LOCUS       DAZPJP010000543          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZPJP010000543 DAZPJP010000000
VERSION     DAZPJP010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.549"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHU9J_004743"
     CDS             complement(<1..>389)
                     /locus_tag="ACHU9J_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1908046.1"
                     /translation="NEGSLLFDFFYKKVRFHIGCLFIRKQLLEENNLFFDEDLRLGED
                     LDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHYRHESFAHERIYSSVMQLY
                     KGNRKEEIHTLLSQNRAYHKTRYLWNV"
BASE COUNT          132 a           67 c           55 g          135 t
ORIGIN      
        1 gaacattcca taaataacga gttttatgat aagctctatt ttgactcaat aatgtatgaa
       61 tttcttcttt cctgttacct ttgtataact gcatcacaga agagtagatt ctttcgtgcg
      121 caaatgactc atgtcgataa tgagtgatgg ttctacatga gttcattaag gaattttctc
      181 tatagttatg cttatacata taatatggaa ccgcatacat atcgcatgta attagcagtc
      241 gatagataaa atccagatct tctcctagtc gtaaatcttc atcaaaaaaa agattattct
      301 cttccagaag ttgttttctt ataaacaagc accctatatg gaatctaacc tttttataaa
      361 aaaagtcgaa aagtaaagat ccttcatta
//
LOCUS       DAZPJP010000544          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZPJP010000544 DAZPJP010000000
VERSION     DAZPJP010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.550"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHU9J_004744"
     CDS             complement(<1..>389)
                     /locus_tag="ACHU9J_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1908047.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAZPJP010000545          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZPJP010000545 DAZPJP010000000
VERSION     DAZPJP010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.551"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /locus_tag="ACHU9J_004745"
     CDS             complement(<1..305)
                     /locus_tag="ACHU9J_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1908048.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          118 a           76 c           80 g          110 t
ORIGIN      
        1 cttgaatcct ctctcggcac ggttgcaatt agcgagtggg tagtatcacc aacagcgcga
       61 aatccgcgtc tgataatgga tatgttctga tataacgtgt tagtaggcac acgcatacct
      121 tcttcttccc agactctggt gaaaaaatct tgttgtgtaa ccacatcagg aaaagcttca
      181 agtagaagtg ataaacatcg actggctggc gttgtcagaa cgacgttaag atcggcgtta
      241 tgtaccgata ttaattttcg gtgctcaggc cagaactcga tattatcgtt aataatccag
      301 tacatacgtc aataaacact aaatcaatcg aaatggagat cacatagttt gctaagtata
      361 ttggtattta cggcataaat atat
//
LOCUS       DAZPJP010000546          373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZPJP010000546 DAZPJP010000000
VERSION     DAZPJP010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.552"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            20..307
                     /locus_tag="ACHU9J_004746"
     CDS             20..307
                     /locus_tag="ACHU9J_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908049.1"
                     /translation="MSTRNIHVNTASYTILVAGREKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
BASE COUNT          105 a           87 c          103 g           78 t
ORIGIN      
        1 agagagagga gaaagctgga tgagtacccg aaatatccac gttaacaccg cgtcgtacac
       61 gattcttgtt gccgggaggg aaaaaaacac cggggaagag tgggacgtcc tggaattcag
      121 cagcctgact gagctgaaaa aatatcgcaa aagccacccg gaaaagatgg ccttcagcta
      181 tagctacgcg ctcagccggg gtgtggatac gcagttccgc catatcaaca ttgcggaagc
      241 cgatcacttc aaacagttcc tgcgtcagat taagcgtgcc ggcctcgata tccgggcgat
      301 ctgctgaccg gatgtgatga atatccctcc tgattgttgc aatcttgtta atagagatcg
      361 ggaggaatga tat
//
LOCUS       DAZPJP010000547          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZPJP010000547 DAZPJP010000000
VERSION     DAZPJP010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.553"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>367
                     /locus_tag="ACHU9J_004747"
     CDS             <1..>367
                     /locus_tag="ACHU9J_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1908050.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAZPJP010000548          362 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZPJP010000548 DAZPJP010000000
VERSION     DAZPJP010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.554"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHU9J_004748"
     tRNA            41..116
                     /locus_tag="ACHU9J_004748"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT           97 a           68 c           93 g          104 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cg
//
LOCUS       DAZPJP010000549          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZPJP010000549 DAZPJP010000000
VERSION     DAZPJP010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.555"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHU9J_004749"
     CDS             <1..>357
                     /locus_tag="ACHU9J_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1908051.1"
                     /translation="DTISVKFYRNEKIECACNFLMDKDAQGYIDLSDFDLINCHFKGD
                     VISKVSFLSSNLQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFS
                     QNILDTVDFSQSQLGHS"
BASE COUNT          118 a           47 c           66 g          126 t
ORIGIN      
        1 gatactataa gtgtaaaatt ctacagaaat gaaaaaatag aatgcgcttg taattttctg
       61 atggataaag atgcgcaggg gtatatcgac ctgtctgatt ttgatttaat aaattgtcat
      121 tttaaaggtg acgttatttc taaggtgtct tttttatcat caaatctaca acatgtaact
      181 ttcgaatgta aagaaattga gaattgcaat tttactaagg caacagttga taatgtcata
      241 tttaaatgtc gacgtttaca caatgtgatt tttctcaaaa cgagtggcga atgtgttgat
      301 tttagccaaa atattcttga tacagttgac ttctcgcaga gtcaacttgg tcatagt
//
LOCUS       DAZPJP010000550          354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZPJP010000550 DAZPJP010000000
VERSION     DAZPJP010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.556"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>354
                     /locus_tag="ACHU9J_004750"
     CDS             <1..>354
                     /locus_tag="ACHU9J_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR1908052.1"
                     /translation="TITNNMTSILRSEFDEIWKAMLTLQFHDILPGSCISRVYHETEK
                     EYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARV
                     NSYGYAVIVPKNKIVN"
BASE COUNT          123 a           64 c           57 g          110 t
ORIGIN      
        1 actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat atggaaagcg
       61 atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag agtttatcat
      121 gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat ttctgatgcg
      181 cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca cattttgttt
      241 aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg gctcaaagca
      301 cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt aaat
//
LOCUS       DAZPJP010000551          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZPJP010000551 DAZPJP010000000
VERSION     DAZPJP010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.557"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>344
                     /locus_tag="ACHU9J_004751"
     CDS             <1..>344
                     /locus_tag="ACHU9J_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1908053.1"
                     /translation="LKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKI
                     VVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCIS
                     FTLRINGKDHDL"
BASE COUNT          100 a           64 c           81 g           99 t
ORIGIN      
        1 ttaaaaaata cagaggagat tcaacgcgcc gtgattctga aaatggaaat gatgccattt
       61 ttagactcgg tgggactggt acttgatgat aataaatatt atctcttttc gcggagggcg
      121 aatgataaaa tcgttgttta tcatcaggaa caagtaaatg gaccgcttgt cgacgagtca
      181 gggcgggtta tttttgccga ttttaaccca tcgaaacgac cgtggtcggt ggcttcagat
      241 gactctaaca acagctggaa tccggcatac aattgctttg atcgtccggg taaaaaatgt
      301 atctctttta cgctacgtat caacggcaaa gatcacgatt tgtt
//
LOCUS       DAZPJP010000552          339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZPJP010000552 DAZPJP010000000
VERSION     DAZPJP010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.558"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHU9J_004752"
     CDS             complement(<1..242)
                     /locus_tag="ACHU9J_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1908054.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          104 a           65 c           70 g          100 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatcct
//
LOCUS       DAZPJP010000553          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZPJP010000553 DAZPJP010000000
VERSION     DAZPJP010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.559"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHU9J_004753"
     CDS             <1..>338
                     /locus_tag="ACHU9J_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908055.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPE"
BASE COUNT           82 a          124 c           86 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaag
//
LOCUS       DAZPJP010000554          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZPJP010000554 DAZPJP010000000
VERSION     DAZPJP010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.560"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(<1..>333)
                     /locus_tag="ACHU9J_004754"
     CDS             complement(<1..>333)
                     /locus_tag="ACHU9J_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1908056.1"
                     /translation="NRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSS
                     DSNKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHG
                     FAPVIHGI"
BASE COUNT           93 a           76 c           61 g          103 t
ORIGIN      
        1 caataccatg aatcaccgga gcgaaaccat gttgtctatc gggcaacatc gcttctgttg
       61 aatttatttt gaggccacga aagttcacac tatcaaaaat atcgccatcg gtataactat
      121 cacctaccgt taaccgtgag cgtaagggaa taatgtcacg ttcagccgac gtattgatat
      181 gctgccattt attgctgtca gatgaattgc tactcccact gttataactc cacgttgagt
      241 tatcgcgtag tcgccaacta ccaatgttga tgccactctg taaattcaaa tatgcatagt
      301 ttgattttcc tgcattatgg ttactacgat tat
//
LOCUS       DAZPJP010000555          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZPJP010000555 DAZPJP010000000
VERSION     DAZPJP010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.561"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHU9J_004755"
                     /pseudo
     CDS             complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHU9J_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          112 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aa
//
LOCUS       DAZPJP010000556          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZPJP010000556 DAZPJP010000000
VERSION     DAZPJP010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.denovo.562"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..330
                     /locus_tag="ACHU9J_004756"
                     /pseudo
     CDS             1..330
                     /locus_tag="ACHU9J_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           82 c           98 g           65 t
ORIGIN      
        1 tatgcagcga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc
       61 atccggataa acgcagtgcc cgtgcgcagc gcgatgactg gctgaagaaa gagatacagc
      121 gcgtatacga tgaaaatcac aaggtatacg gtgtaaagtc tggcgtcagt tgttacggga
      181 aggtatcaga gtggccagat gcactgtggc acgtctcatg gcggttatgg gacttgccgg
      241 tgttctccgg ggtaaaaagg tccgtacgac catcagccgg aaagccgttg ccgcaggcga
      301 ccgcgtaaac cgtcagttcg tggcagaacg
//
LOCUS       DAZPJP010000557         9666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.guided.150, whole genome shotgun sequence.
ACCESSION   DAZPJP010000557 DAZPJP010000000
VERSION     DAZPJP010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.guided.150"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            complement(35..205)
                     /locus_tag="ACHU9J_004757"
                     /pseudo
     CDS             complement(35..205)
                     /locus_tag="ACHU9J_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHU9J_004758"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="ACHU9J_004758"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1908057.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="ACHU9J_004759"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="ACHU9J_004759"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1908058.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="ACHU9J_004760"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="ACHU9J_004760"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1908059.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="ACHU9J_004761"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="ACHU9J_004761"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1908060.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="ACHU9J_004762"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="ACHU9J_004762"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1908061.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5100..6233
                     /locus_tag="ACHU9J_004763"
     CDS             5100..6233
                     /locus_tag="ACHU9J_004763"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1908062.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="ACHU9J_004764"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="ACHU9J_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1908063.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHU9J_004765"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="ACHU9J_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1908064.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHU9J_004766"
     CDS             complement(7344..7490)
                     /gene="ecnB"
                     /locus_tag="ACHU9J_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1908065.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHU9J_004767"
     CDS             complement(7601..7726)
                     /gene="ecnA"
                     /locus_tag="ACHU9J_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1908066.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHU9J_004768"
     CDS             complement(7778..8344)
                     /gene="efp"
                     /locus_tag="ACHU9J_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1908067.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8386..9414
                     /gene="epmB"
                     /locus_tag="ACHU9J_004769"
     CDS             8386..9414
                     /gene="epmB"
                     /locus_tag="ACHU9J_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1908068.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2459 a         2479 c         2470 g         2258 t
ORIGIN      
        1 accatcatac actaaatcag taagttggca gcatcaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacccggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcacgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
     2281 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaacttcc tcaaacacac cctcgccttc cgtgatgctg atggcacgac tcgcctagag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttactgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgacagt agtgaataat
     6421 ttcgacgcta aacgctatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagtctgc gtgatgacgg cggcctgaat
     6541 gtcatcaata aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac acgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgtcattgc acttgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacagtaat aacacttggc
     7081 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt gtgactatcg caaaaaaaca aacggcacga
     7321 cataactgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcacc agaaatcgcg
     7381 ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc agttaatact
     7441 gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat aacgtcttcc
     7501 ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa catcgccaaa
     7561 aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca gcgcgggaga
     7621 tggagttgcc gagatgtttg atgtcttcgc cgaaaccgcg agcggtgtta cagcccgtga
     7681 gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct gacctgaata
     7741 agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga gagacgtatt
     7801 caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc ggaactttaa
     7861 ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta tcacctttca
     7921 ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta acggagatcg
     7981 gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat ttagcgttgt
     8041 cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc atgaagtgcc
     8101 agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca gcgccttcag
     8161 cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga cgacgcagtt
     8221 taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct tcaaccgcgt
     8281 aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta tagtacgttg
     8341 ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc gcatattgta
     8401 accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt tgtgaccgat
     8461 cctggtgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt ggccggacgc
     8521 agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg catggagaaa
     8581 ggcaatccgg acgatcctat tttgcgtcag gtacttacct cgcaagatga gtttgtcgtc
     8641 gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc tggtttgttg
     8701 cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt aaattgccgc
     8761 tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg taactggcag
     8821 actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt ctccggcggc
     8881 gatccgctga tggcgaaaga tcacgagctg gactggttgc tcacacaact ggaagccatc
     8941 ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc ggcacgtatc
     9001 accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct ggtgaatcac
     9061 atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa attgcgccgt
     9121 gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga taacgcacaa
     9181 acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta ttacctgcat
     9241 gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga agcacggcag
     9301 attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact ggcgcgagaa
     9361 attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca gtaactgatt
     9421 aaacagggaa gttaaacatt agcctgatgc gctacgctta tcaggcctac attgtgcatt
     9481 gaaattgaat ggatttgcaa tgaactgtag gccggataag gcgtttacgc cgcatccggc
     9541 aagagcaaca aacgcaagta cagcaacctg tcattaaact gggaaattta atctttgccc
     9601 gttgcataac ctttaattat tcatatacat tgctgttgtt actctttata tagccaacac
     9661 caataa
//
LOCUS       DAZPJP010000558         6808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.guided.209, whole genome shotgun sequence.
ACCESSION   DAZPJP010000558 DAZPJP010000000
VERSION     DAZPJP010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.guided.209"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            <1..837
                     /locus_tag="ACHU9J_004770"
     CDS             <1..837
                     /locus_tag="ACHU9J_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR1908069.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(944..1348)
                     /locus_tag="ACHU9J_004771"
                     /pseudo
     CDS             complement(944..1348)
                     /locus_tag="ACHU9J_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1392..1781)
                     /locus_tag="ACHU9J_004772"
     CDS             complement(1392..1781)
                     /locus_tag="ACHU9J_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908070.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1999..2439
                     /locus_tag="ACHU9J_004773"
     CDS             1999..2439
                     /locus_tag="ACHU9J_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1908071.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(3184..3333)
                     /locus_tag="ACHU9J_004774"
     CDS             complement(3184..3333)
                     /locus_tag="ACHU9J_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1908072.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            3904..4062
                     /locus_tag="ACHU9J_004775"
     CDS             3904..4062
                     /locus_tag="ACHU9J_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1908073.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            4522..5337
                     /gene="sul2"
                     /locus_tag="ACHU9J_004776"
     CDS             4522..5337
                     /gene="sul2"
                     /locus_tag="ACHU9J_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1908074.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5398..5925
                     /locus_tag="ACHU9J_004777"
                     /pseudo
     CDS             5398..5925
                     /locus_tag="ACHU9J_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            5946..6419
                     /gene="dfrA14"
                     /locus_tag="ACHU9J_004778"
     CDS             5946..6419
                     /gene="dfrA14"
                     /locus_tag="ACHU9J_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR1908075.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            6494..6769
                     /locus_tag="ACHU9J_004779"
                     /pseudo
     CDS             6494..6769
                     /locus_tag="ACHU9J_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
BASE COUNT         1670 a         1782 c         1761 g         1595 t
ORIGIN      
        1 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
       61 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
      121 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
      181 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
      241 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
      301 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
      361 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
      421 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
      481 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
      541 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
      601 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
      661 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
      721 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
      781 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
      841 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
      901 aaagccttag cgtaggattc cgccccttcc cgcaaacgac cccaaacagg aaacgcagct
      961 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     1021 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     1081 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     1141 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     1201 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     1261 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     1321 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     1381 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     1441 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     1501 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     1561 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     1621 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     1681 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     1741 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     1801 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     1861 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     1921 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     1981 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     2041 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     2101 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     2161 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     2221 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     2281 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     2341 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     2401 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     2461 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     2521 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     2581 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     2641 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     2701 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     2761 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     2821 aagtgatgta taagtgatgt aaaagtgatg tatgaactga aggagcgatg taattctgta
     2881 gtcaggaaga ataacccgtt catgcaggca tgaacgatac agtgaggaac gctttttttg
     2941 tcacgatctc cccttaacct gcgtaaatca cccgccatgg ttgattattt ttcaacctca
     3001 aaatggtcat acacctaacg cagccgcgat tttcatcgca actgcatgaa ttaaaagaaa
     3061 aaaaaggaat atttcgtttt cacgagtaat acccgggtat taaaaatcca ctgtaattac
     3121 atgaaaataa agaaataaaa ggtaataccg gagtattacc acgtacgcgg gcaagcgaaa
     3181 aagctataac gcttcccggc cgacaggcga cccaaaatcc acaggggcat gcaggttctc
     3241 ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt
     3301 cgaatccagt tgttcatcat attcagatgg catggggtga tatccagtag ctgagaggtt
     3361 gttagcgggt ttcggggcgc agccctgaac cagtcatgta gcgctagcgg agtgtatact
     3421 ggcttactat gttgccacga ttgcgaaaat cagtgaagtg cttcatgtgg caggagaaaa
     3481 aaggcagcac cggcgcgtca gcagaatatg cgatacagga gatattccgc ttcctcgctc
     3541 actgactcgc tacgctcggt cgttcggctg cggcgagcgg taccggctta cggacgggga
     3601 cggaaatttc ctggaagata ccaggaagac acttagcaaa gaagagataa ggccacggcg
     3661 aagccgtttt tccataggct ccgcccccct gacaagcatc gcaaaatctg acgctcaaat
     3721 cagtggtggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     3781 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     3841 cggtttctca ttccacgcct gacactcagt tccgtgaagg cagttcgctc caagctggac
     3901 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tgtccggtaa ctatcaactt
     3961 gagtccaacc cggaaagaca cgacaaaacg ccactggcag cagccattag taacaggagt
     4021 gcacgagaag atagattaag aggtcttgaa gtagaggcct gactgcggct acactgaaag
     4081 aacagttttg gtaatggcgg tctcgtgaac cctgttaccg ggttaagcag ttccccaact
     4141 gacttaacct tcgaacaaac cgcctcccca ggcggttttt tcgtttacag agcaagagat
     4201 tacgattcat tgaaaaggat ctcaagaaga tcctttgtag attactatca attaatcttc
     4261 cgaacatgac accagtcact gttgattacg gttaaaccgc tccgtaacag gtttccctcg
     4321 tattggtgac ttgcagcacc aaagaagcaa aaaagataaa tatatctact tattgagaga
     4381 tgatatattt atttccaaaa tgctacagat acatcatcat gaaacggatc accgcaaaca
     4441 ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgagactc atgctcgatt
     4501 attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat cgtcaacata
     4561 acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc cattgcgcag
     4621 gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc atccagcaat
     4681 cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac
     4741 gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc
     4801 tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg
     4861 ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac
     4921 gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc
     4981 tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt
     5041 gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg
     5101 cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa
     5161 tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc gacactcgct
     5221 gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc
     5281 ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg
     5341 cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga acctccattg
     5401 aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt cagaggcgga
     5461 gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct
     5521 gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct caaaggtcga
     5581 ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc atgcttggtg
     5641 ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt gctcaaagcg
     5701 tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga tcaatgtccg
     5761 tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc
     5821 gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga tcttttggct
     5881 cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgttaa cccaggatga
     5941 gaaccttgaa agtatcattg atagctgcga aagcgaaaaa cggcgtgatt ggttgcggtc
     6001 cagacatacc ctggtccgcg aaaggggagc agctactttt taaagcattg acctacaatc
     6061 agtggcttct ggtgggtcgc aagacgtttg aatctatggg cgcactcccc aataggaaat
     6121 acgcggtcgt tacccgctca ggttggacat caaatgatga caatgtagtt gtatttcagt
     6181 caatcgaaga ggccatggac aggctagctg aattcaccgg tcacgttata gtgtctggtg
     6241 gcggagaaat ttaccgagaa acattaccca tggcctctac gctccactta tcgacgatcg
     6301 acatcgagcc agagggggat gttttcttcc cgagtattcc aaataccttc gaagttgttt
     6361 ttgagcaaca ctttacttca aacattaact attgctatca aatttggaaa aagggttaac
     6421 aaagctatgc aatcgacggc aaaaagcttc gttcgcttcg cgcactacgc ctttttccgc
     6481 gattgatagc gacgatatgg ttgtttgcca tggtgatccc tgcatgccga acttcatggt
     6541 ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg gaacagcaga
     6601 tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg cagcgccaga
     6661 tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag cccccgaccg
     6721 cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat gttcatgccg
     6781 cctgtttttc ctgctcattg gcacgttt
//
LOCUS       DAZPJP010000559         6474 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2003678
            SAMN43277503-rid23489743.guided.222, whole genome shotgun sequence.
ACCESSION   DAZPJP010000559 DAZPJP010000000
VERSION     DAZPJP010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277503
            Sequence Read Archive: SRR30464568
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396919.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396919.1
            Genome Coverage       :: 81x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 10:31:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,786
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 125 of 284
            Pseudo Genes (incomplete)         :: 106 of 284
            Pseudo Genes (internal stop)      :: 84 of 284
            Pseudo Genes (multiple problems)  :: 30 of 284
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277503-rid23489743.guided.222"
                     /strain="M2003678"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-03-08"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHU9J_004780"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHU9J_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHU9J_004781"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHU9J_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1908076.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHU9J_004782"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHU9J_004782"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1908077.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHU9J_004783"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHU9J_004783"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1908078.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHU9J_004784"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHU9J_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1908079.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHU9J_004785"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHU9J_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1908080.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHU9J_004786"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHU9J_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1908081.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1581 a         1588 c         1659 g         1646 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aaga
//
